LOCUS       DAYYBH010000001       126131 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000001 DAYYBH010000000
VERSION     DAYYBH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 126131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 126131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..126131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.001"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..976)
                     /locus_tag="ACHAUG_000001"
     CDS             complement(<1..976)
                     /locus_tag="ACHAUG_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8479132.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
     gene            complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACHAUG_000002"
     CDS             complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACHAUG_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8479133.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACHAUG_000003"
     CDS             complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACHAUG_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8479134.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2753..3391)
                     /locus_tag="ACHAUG_000004"
     CDS             complement(2753..3391)
                     /locus_tag="ACHAUG_000004"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8479135.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACHAUG_000005"
     CDS             complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACHAUG_000005"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8479136.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACHAUG_000006"
     CDS             complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACHAUG_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8479137.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACHAUG_000007"
     CDS             complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACHAUG_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8479138.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7185..7925)
                     /locus_tag="ACHAUG_000008"
     CDS             complement(7185..7925)
                     /locus_tag="ACHAUG_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8479139.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACHAUG_000009"
     CDS             complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACHAUG_000009"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8479140.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACHAUG_000010"
     CDS             complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACHAUG_000010"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8479141.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACHAUG_000011"
     CDS             complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACHAUG_000011"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8479142.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACHAUG_000012"
     CDS             complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACHAUG_000012"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8479143.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACHAUG_000013"
     CDS             complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACHAUG_000013"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8479144.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACHAUG_000014"
     CDS             complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACHAUG_000014"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8479145.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14805..15089)
                     /locus_tag="ACHAUG_000015"
     CDS             complement(14805..15089)
                     /locus_tag="ACHAUG_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479146.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15107..15997)
                     /locus_tag="ACHAUG_000016"
     CDS             complement(15107..15997)
                     /locus_tag="ACHAUG_000016"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8479147.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16017..16235)
                     /locus_tag="ACHAUG_000017"
     CDS             complement(16017..16235)
                     /locus_tag="ACHAUG_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8479148.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16205..17905)
                     /locus_tag="ACHAUG_000018"
     CDS             complement(16205..17905)
                     /locus_tag="ACHAUG_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8479149.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18172..18945
                     /locus_tag="ACHAUG_000019"
     CDS             18172..18945
                     /locus_tag="ACHAUG_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479150.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACHAUG_000020"
     CDS             complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACHAUG_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8479151.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19661..19763)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19769..20740)
                     /locus_tag="ACHAUG_000021"
     CDS             complement(19769..20740)
                     /locus_tag="ACHAUG_000021"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8479152.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACHAUG_000022"
     CDS             complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACHAUG_000022"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8479153.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACHAUG_000023"
     CDS             complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACHAUG_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8479154.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23695..24519)
                     /locus_tag="ACHAUG_000024"
     CDS             complement(23695..24519)
                     /locus_tag="ACHAUG_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8479155.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24492..25172)
                     /locus_tag="ACHAUG_000025"
     CDS             complement(24492..25172)
                     /locus_tag="ACHAUG_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8479156.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25195..26133)
                     /locus_tag="ACHAUG_000026"
     CDS             complement(25195..26133)
                     /locus_tag="ACHAUG_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8479157.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACHAUG_000027"
     CDS             complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACHAUG_000027"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8479158.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACHAUG_000028"
     CDS             complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACHAUG_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8479159.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30097..30903
                     /gene="proC"
                     /locus_tag="ACHAUG_000029"
     CDS             30097..30903
                     /gene="proC"
                     /locus_tag="ACHAUG_000029"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8479160.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACHAUG_000030"
     CDS             complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACHAUG_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8479161.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACHAUG_000031"
     CDS             complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACHAUG_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8479162.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34107..35348)
                     /locus_tag="ACHAUG_000032"
     CDS             complement(34107..35348)
                     /locus_tag="ACHAUG_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8479163.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACHAUG_000033"
     CDS             complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACHAUG_000033"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8479164.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36375..37298)
                     /locus_tag="ACHAUG_000034"
     CDS             complement(36375..37298)
                     /locus_tag="ACHAUG_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8479165.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38037..39323)
                     /locus_tag="ACHAUG_000035"
     CDS             complement(38037..39323)
                     /locus_tag="ACHAUG_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8479166.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39316..40584)
                     /locus_tag="ACHAUG_000036"
     CDS             complement(39316..40584)
                     /locus_tag="ACHAUG_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8479167.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40836..41297
                     /gene="mscL"
                     /locus_tag="ACHAUG_000037"
     CDS             40836..41297
                     /gene="mscL"
                     /locus_tag="ACHAUG_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8479168.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41356..41802)
                     /locus_tag="ACHAUG_000038"
     CDS             complement(41356..41802)
                     /locus_tag="ACHAUG_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8479169.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41895..42140)
                     /locus_tag="ACHAUG_000039"
     CDS             complement(41895..42140)
                     /locus_tag="ACHAUG_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8479170.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42255..42833)
                     /locus_tag="ACHAUG_000040"
     CDS             complement(42255..42833)
                     /locus_tag="ACHAUG_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8479171.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42910..43440)
                     /locus_tag="ACHAUG_000041"
     CDS             complement(42910..43440)
                     /locus_tag="ACHAUG_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8479172.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACHAUG_000042"
     CDS             complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACHAUG_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8479173.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44297..44692)
                     /locus_tag="ACHAUG_000043"
     CDS             complement(44297..44692)
                     /locus_tag="ACHAUG_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8479174.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACHAUG_000044"
     CDS             complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACHAUG_000044"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8479175.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACHAUG_000045"
     CDS             complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACHAUG_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8479176.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(46720..46959)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACHAUG_000046"
     CDS             complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACHAUG_000046"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8479177.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48586..49743)
                     /locus_tag="ACHAUG_000047"
     CDS             complement(48586..49743)
                     /locus_tag="ACHAUG_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8479178.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACHAUG_000048"
     CDS             complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACHAUG_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8479179.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51210..51737)
                     /locus_tag="ACHAUG_000049"
     CDS             complement(51210..51737)
                     /locus_tag="ACHAUG_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8479180.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            51967..52773
                     /gene="proB"
                     /locus_tag="ACHAUG_000050"
     CDS             51967..52773
                     /gene="proB"
                     /locus_tag="ACHAUG_000050"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8479181.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            52770..54017
                     /locus_tag="ACHAUG_000051"
     CDS             52770..54017
                     /locus_tag="ACHAUG_000051"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8479182.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54093..54437
                     /locus_tag="ACHAUG_000052"
     CDS             54093..54437
                     /locus_tag="ACHAUG_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479183.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(54554..54880)
                     /locus_tag="ACHAUG_000053"
     CDS             complement(54554..54880)
                     /locus_tag="ACHAUG_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479184.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(55495..56697)
                     /locus_tag="ACHAUG_000054"
     CDS             complement(55495..56697)
                     /locus_tag="ACHAUG_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8479185.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            56814..57329
                     /locus_tag="ACHAUG_000055"
     CDS             56814..57329
                     /locus_tag="ACHAUG_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8479186.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(57528..57974)
                     /locus_tag="ACHAUG_000056"
     CDS             complement(57528..57974)
                     /locus_tag="ACHAUG_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8479187.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58021..59499)
                     /gene="msr(C)"
                     /locus_tag="ACHAUG_000057"
     CDS             complement(58021..59499)
                     /gene="msr(C)"
                     /locus_tag="ACHAUG_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8479188.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(59853..60482)
                     /locus_tag="ACHAUG_000058"
     CDS             complement(59853..60482)
                     /locus_tag="ACHAUG_000058"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8479189.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(60466..60930)
                     /locus_tag="ACHAUG_000059"
     CDS             complement(60466..60930)
                     /locus_tag="ACHAUG_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8479190.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(61417..62685)
                     /locus_tag="ACHAUG_000060"
     CDS             complement(61417..62685)
                     /locus_tag="ACHAUG_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8479191.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(62916..63653)
                     /locus_tag="ACHAUG_000061"
     CDS             complement(62916..63653)
                     /locus_tag="ACHAUG_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8479192.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(63720..64289)
                     /locus_tag="ACHAUG_000062"
     CDS             complement(63720..64289)
                     /locus_tag="ACHAUG_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8479193.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            64417..65289
                     /locus_tag="ACHAUG_000063"
     CDS             64417..65289
                     /locus_tag="ACHAUG_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8479194.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            65631..66503
                     /locus_tag="ACHAUG_000064"
     CDS             65631..66503
                     /locus_tag="ACHAUG_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8479195.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            66500..68458
                     /gene="nagE"
                     /locus_tag="ACHAUG_000065"
     CDS             66500..68458
                     /gene="nagE"
                     /locus_tag="ACHAUG_000065"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8479196.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(68790..69641)
                     /locus_tag="ACHAUG_000066"
     CDS             complement(68790..69641)
                     /locus_tag="ACHAUG_000066"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8479197.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            69764..70231
                     /locus_tag="ACHAUG_000067"
     CDS             69764..70231
                     /locus_tag="ACHAUG_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8479198.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(70494..70976)
                     /locus_tag="ACHAUG_000068"
     CDS             complement(70494..70976)
                     /locus_tag="ACHAUG_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8479199.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71076..71939)
                     /locus_tag="ACHAUG_000069"
     CDS             complement(71076..71939)
                     /locus_tag="ACHAUG_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8479200.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            72537..73412
                     /locus_tag="ACHAUG_000070"
     CDS             72537..73412
                     /locus_tag="ACHAUG_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8479201.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(73557..73709)
                     /locus_tag="ACHAUG_000071"
                     /pseudo
     CDS             complement(73557..73709)
                     /locus_tag="ACHAUG_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(73977..75308)
                     /locus_tag="ACHAUG_000072"
     CDS             complement(73977..75308)
                     /locus_tag="ACHAUG_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8479202.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(75457..76170)
                     /locus_tag="ACHAUG_000073"
     CDS             complement(75457..76170)
                     /locus_tag="ACHAUG_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8479203.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            76345..77769
                     /locus_tag="ACHAUG_000074"
     CDS             76345..77769
                     /locus_tag="ACHAUG_000074"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8479204.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            78068..78775
                     /locus_tag="ACHAUG_000075"
     CDS             78068..78775
                     /locus_tag="ACHAUG_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8479205.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            78762..79085
                     /locus_tag="ACHAUG_000076"
     CDS             78762..79085
                     /locus_tag="ACHAUG_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8479206.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(79174..80211)
                     /locus_tag="ACHAUG_000077"
     CDS             complement(79174..80211)
                     /locus_tag="ACHAUG_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8479207.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(80532..81515)
                     /locus_tag="ACHAUG_000078"
     CDS             complement(80532..81515)
                     /locus_tag="ACHAUG_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8479208.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(81930..82700)
                     /locus_tag="ACHAUG_000079"
     CDS             complement(81930..82700)
                     /locus_tag="ACHAUG_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8479209.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(82693..83628)
                     /locus_tag="ACHAUG_000080"
     CDS             complement(82693..83628)
                     /locus_tag="ACHAUG_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8479210.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(83944..85593)
                     /locus_tag="ACHAUG_000081"
     CDS             complement(83944..85593)
                     /locus_tag="ACHAUG_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8479211.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(85710..88313)
                     /locus_tag="ACHAUG_000082"
     CDS             complement(85710..88313)
                     /locus_tag="ACHAUG_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8479212.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(88325..89374)
                     /locus_tag="ACHAUG_000083"
     CDS             complement(88325..89374)
                     /locus_tag="ACHAUG_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8479213.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(89400..89714)
                     /locus_tag="ACHAUG_000084"
     CDS             complement(89400..89714)
                     /locus_tag="ACHAUG_000084"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8479214.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(89730..90176)
                     /locus_tag="ACHAUG_000085"
     CDS             complement(89730..90176)
                     /locus_tag="ACHAUG_000085"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8479215.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(90177..92099)
                     /locus_tag="ACHAUG_000086"
     CDS             complement(90177..92099)
                     /locus_tag="ACHAUG_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8479216.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(92311..93207)
                     /locus_tag="ACHAUG_000087"
     CDS             complement(92311..93207)
                     /locus_tag="ACHAUG_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8479217.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(93200..93880)
                     /locus_tag="ACHAUG_000088"
     CDS             complement(93200..93880)
                     /locus_tag="ACHAUG_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8479218.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(93881..94663)
                     /locus_tag="ACHAUG_000089"
     CDS             complement(93881..94663)
                     /locus_tag="ACHAUG_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8479219.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(94656..95573)
                     /locus_tag="ACHAUG_000090"
     CDS             complement(94656..95573)
                     /locus_tag="ACHAUG_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8479220.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(95737..95991)
                     /locus_tag="ACHAUG_000091"
     CDS             complement(95737..95991)
                     /locus_tag="ACHAUG_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479221.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            96848..100075
                     /gene="fss3"
                     /locus_tag="ACHAUG_000092"
     CDS             96848..100075
                     /gene="fss3"
                     /locus_tag="ACHAUG_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8479222.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            100188..100502
                     /locus_tag="ACHAUG_000093"
     CDS             100188..100502
                     /locus_tag="ACHAUG_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8479223.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            100515..100889
                     /locus_tag="ACHAUG_000094"
     CDS             100515..100889
                     /locus_tag="ACHAUG_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8479224.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            100899..101234
                     /locus_tag="ACHAUG_000095"
     CDS             100899..101234
                     /locus_tag="ACHAUG_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8479225.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            101317..102657
                     /locus_tag="ACHAUG_000096"
     CDS             101317..102657
                     /locus_tag="ACHAUG_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8479226.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            102735..103409
                     /locus_tag="ACHAUG_000097"
     CDS             102735..103409
                     /locus_tag="ACHAUG_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8479227.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            103402..103566
                     /locus_tag="ACHAUG_000098"
     CDS             103402..103566
                     /locus_tag="ACHAUG_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479228.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            103642..104784
                     /gene="dcm"
                     /locus_tag="ACHAUG_000099"
                     /pseudo
     CDS             103642..104784
                     /gene="dcm"
                     /locus_tag="ACHAUG_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            104774..105064
                     /locus_tag="ACHAUG_000100"
     CDS             104774..105064
                     /locus_tag="ACHAUG_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479229.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            105323..106507
                     /gene="mobT"
                     /locus_tag="ACHAUG_000101"
     CDS             105323..106507
                     /gene="mobT"
                     /locus_tag="ACHAUG_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8479230.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            106504..106641
                     /locus_tag="ACHAUG_000102"
     CDS             106504..106641
                     /locus_tag="ACHAUG_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8479231.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            107387..109297
                     /gene="ltrA"
                     /locus_tag="ACHAUG_000103"
     CDS             107387..109297
                     /gene="ltrA"
                     /locus_tag="ACHAUG_000103"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8479232.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            109401..109625
                     /locus_tag="ACHAUG_000104"
     CDS             109401..109625
                     /locus_tag="ACHAUG_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479233.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            109638..110141
                     /locus_tag="ACHAUG_000105"
     CDS             109638..110141
                     /locus_tag="ACHAUG_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8479234.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            110201..110590
                     /locus_tag="ACHAUG_000106"
     CDS             110201..110590
                     /locus_tag="ACHAUG_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8479235.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            110577..113024
                     /locus_tag="ACHAUG_000107"
     CDS             110577..113024
                     /locus_tag="ACHAUG_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8479236.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            113029..115152
                     /locus_tag="ACHAUG_000108"
     CDS             113029..115152
                     /locus_tag="ACHAUG_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8479237.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            115149..116153
                     /locus_tag="ACHAUG_000109"
     CDS             115149..116153
                     /locus_tag="ACHAUG_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8479238.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            116172..117083
                     /locus_tag="ACHAUG_000110"
     CDS             116172..117083
                     /locus_tag="ACHAUG_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8479239.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            117270..117526
                     /locus_tag="ACHAUG_000111"
                     /pseudo
     CDS             117270..117526
                     /locus_tag="ACHAUG_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(117743..117958)
                     /locus_tag="ACHAUG_000112"
     CDS             complement(117743..117958)
                     /locus_tag="ACHAUG_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479240.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(118207..118425)
                     /locus_tag="ACHAUG_000113"
                     /pseudo
     CDS             complement(118207..118425)
                     /locus_tag="ACHAUG_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(118720..120159)
                     /locus_tag="ACHAUG_000114"
     CDS             complement(118720..120159)
                     /locus_tag="ACHAUG_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8479241.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            complement(120747..121379)
                     /locus_tag="ACHAUG_000115"
     CDS             complement(120747..121379)
                     /locus_tag="ACHAUG_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8479242.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            complement(121728..123131)
                     /locus_tag="ACHAUG_000116"
     CDS             complement(121728..123131)
                     /locus_tag="ACHAUG_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8479243.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            complement(123243..124586)
                     /locus_tag="ACHAUG_000117"
     CDS             complement(123243..124586)
                     /locus_tag="ACHAUG_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8479244.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(124720..>126131)
                     /locus_tag="ACHAUG_000118"
     CDS             complement(124720..>126131)
                     /locus_tag="ACHAUG_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8479245.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
BASE COUNT        37153 a        25621 c        22874 g        40483 t
ORIGIN      
        1 ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgattcttct gttgaagatt
       61 caggtgttgt tgattcttct gttgctgaag attgtgtagc tgatgatgat tcttcagtca
      121 ttgaagattg tgtagctgat gattcttctg ttgctgagct ttgtgcagta gatgaagctt
      181 gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc tttttcagct ttttcttgtg
      241 cagcttgttg acgagcttgt tctgctagac gttgttgttc acgaatacga gcttgctctg
      301 cttcagcttc tgctttttga cggttcaagt cagctttctt gtcttcagca gttgcttgtt
      361 cagcagctaa tgaagttttc agtacgttca aatcagcttg tttagataaa agatcgcctt
      421 tttgagattc caaagcagct tggttttcag ctaattcttt tagtttagca tcgttttcag
      481 cttttttgtc ttctacagct tttttatctt gtttttgttg ttccatcaag tcgttgtttg
      541 cttttaccat cgttgtcatt gcttgaacgc gtccaatagc atctgctaat gaatcagcat
      601 tcaatacagc atcgatgtag tttgagcttg tgttgcttac ttgtgcttcg cgtgcttgtt
      661 tttggattgt atcttcacgt ttttcaatac gttcttgtaa atctgcaatg tcttttacta
      721 attgtgcaga ttcttgacgc aatgtatctt gttttgctaa taggtcttgt gcttgtgtgt
      781 tgatttcaga aacttggctt tctaaggcat cgatttgtga ttgtgcatct gcttgttgat
      841 ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg agaatcaaaa tcgtccgccg
      901 cggcagcgat cggtgatgct acggcagtca aggtcattga acataccatt actgctgata
      961 ttaaactctt tttcactctt cattcctccg actggcttaa tttaataaat atctaaatat
     1021 atttaagtct ttcttaacga tataaaaatt gtaccatagc agtgtgtcaa cgatataata
     1081 gttatgttac aaaaatgttt caaaacaact tttaaaaatg acaaaactgt tcgtttcaga
     1141 aaatgatcat gaaaaagccg cgttaagtct agcatttctt aagataacaa cgattctttt
     1201 tcgctttcga cctaaaaata aaaaaagaaa ttattcatca ctgaataatt tcttaaaggg
     1261 aaaaatgaat aatacaaata taatcatgtt caacagcagt gtaggtccta aaaaccttgt
     1321 aataaaatac gtgttattta ctactgctaa tttaaaaatc aactggacca ccatcgtaaa
     1381 caattcataa cccgtgacaa agatgatcat actgaaaaat tcagtaaaga tgttgacatg
     1441 gataacgtct ttcattacat acatcaacca tacaattaaa gggagagcta ctgcatagat
     1501 accgataacg ccaatatagt aagcgtcata gatagccccg agaacaatcg tggtaatcag
     1561 taaataacgt ttttcaaacg ccatactgca acataataat gccaatatca gaaagtgggc
     1621 atttgacata taggtgcctt tcgaccactc ccctagcatt cttgttaaat gaccatctat
     1681 caacatcaaa aagaacaaaa cgattggcag atagtacttc attgtttctt tcttcaacat
     1741 ggactaatcc tccgctagcc gttggacaat cgtcacaact gacacgtcat acatttctgc
     1801 ataaggtttc acataaactt cccgatccag cccataacta tctggtttcg ttttaataac
     1861 cgtaccgatc ggcaagttag ctggagaatt ccctccaaga ccggatgtct ggacaacatc
     1921 cccttctttg atatccgtat caccagttaa ttgggtcacc actaaggcat ggagcttttc
     1981 atcatagttt ttaagcaaac caaacgcttc gccattagcc gaagataccc gtactggaaa
     2041 atgattggag ctttcattag aggatgacag taattcgatt ttagacgaag ccgtattgac
     2101 ctcgatcact cggccaatca atcctttttg cgacatgact gccatatttg cttcgatacc
     2161 gtctttagaa cccttgtcta cgatcagcat gtcctgccaa ctgtcaggcg aacgagtaat
     2221 gacatttgcc gttacttttt cgtaactagt cagcgtctga ttcaactcaa gttctttttt
     2281 caacgcttcg atttctctct cgtaattctt gttttgctgt gaaacgtcat catagctatc
     2341 gattttttct tttagccgct cattttcggc ataagttgaa aaaagattat ggacagaatc
     2401 tactccattt cgtacccacc gtactggggc agagattgtt ttatcaacca ttgcaacact
     2461 gtcattaacg atggattgga agaaattggg ctttccttca ttcgctcgct gcgcagcggt
     2521 cacacttagt atcgtcacca cgatgataac aataaccaag gtaataataa tatttttatt
     2581 aggattgaac ttcttcacaa agacacctcg aattaattag actcttgtgt tcaattttat
     2641 cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta agcttttttt tagcaaataa
     2701 tagattctgt catttttagc ctgaaagaca aattttttta tttgtttatg gatcaatctt
     2761 tgctttgaat aactgcaatc ataccatctg aaaaagaaaa ttctgcggta tctgaaagaa
     2821 attcgttact tgcataagat gtcggatatt ctacttcttt attttccaat acatacttgc
     2881 tgccttttaa tgtaagattc gagacgggag tcaagcagca ataagctaaa tacttcatat
     2941 ctggtatctg tcttactata tgctctccag gcaaataata agacaaataa ttttgacaat
     3001 ctctgataat aagctgatgg gcaaagggtt tgaagcgagg ttccatacct aaccagagat
     3061 tggctaaaag atgatccaat cttccgcccg tagctccaat caaagtgact tccgcttgcg
     3121 ggtaacgttc aaatgttttt aagataccta actgtgtatc tgtatcgtct ttttctgcag
     3181 gggaacgggt atactctttg gcttttgcaa atacttcctt ctgctcttcg ttcgacaatg
     3241 aatcaaaatc acccactgcc agatctactg ggtagcctgc ttgaagcagg tacagcgcac
     3301 cacggtctat cccgacatag gcatcgtatt tggacagcag agactgcggc cagttttgcg
     3361 gattacctcc agcagctaat aaaattttca tttagtctaa tgcctctttt aatcgttcga
     3421 tttgtccgac tggattttcg gctccataga tatatgatcc tgcaacgaag acatccgccc
     3481 ctgcatcttt acagattttt gctgtttctg gaacgattcc gccatctact tcgattgcat
     3541 aagtccaatt gttctctcta cgtagatcag cgagttccgt aattttttct actgtttctt
     3601 caataaagct ttgtccgccg aacccaggat tgacagtcat gactagtact tgatcagcca
     3661 aaggcaaaac atgtttgatc atggagactg gtgttcctgg attgataact actccagctt
     3721 taactcctaa gctttttgtc atttgaagtg cccgatgaat atgaggagtt gcttcgacgt
     3781 ggacagtaat gatatctgct ccagcttttg caaatgctgg aatgtagttt tctggatcaa
     3841 cgatcatcag atggacgtct aacggtaatt tagtaacagg acggattgcc tgaacaatat
     3901 ttggccctaa tgtgatattt gggacaaatc ggccatccat tacatctacg tggatgtaat
     3961 ctgcgcctcc tttttccact aactcaatgt ctctttgtaa attggcaaaa tctgcactca
     4021 aaatggatgg tgctatcttc attcttgttt cctcctattt tcgtttacct tctttttttg
     4081 catacattgg ttttcttttt tcgatttctt gtaagaattg caaataattg tcgtaacgtg
     4141 tctgtgcgat accacctgtt tctactctat gttttacttc gcagccaggt tctttagcat
     4201 gcatgcactc tctaaatttg caatggacag aagcttctac aaattcagga aattgtctcg
     4261 gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct aaatcctggt gtatctgcta
     4321 ctaacccgcc atagatagga atcagttcca catgacgtgt tgtatgccgt cctcttccaa
     4381 gtgcatcaga aatctctcct gttgctaaat tcagttccgg tgatatttga ttcagtagcg
     4441 tcgattttcc tgctccagac tgacccataa agaccgatag ccgttctggg aagtaacgga
     4501 tcagttctgt cgtatcaccc gtatgattgg gtacaatcac cggatagcct attttagtat
     4561 aggtttgctt gatctcttcg attttttctg ggtcgtctga caagtcggtc tttgtcaaaa
     4621 aaataatcgg ttcgatcgac tcgtattcca aggtaactag aaagcggtca agtagattat
     4681 aagaaaatgc aggctctacg atacttgtga caatgattcc aagatcaaca tttgtgactg
     4741 gtgggcgaac taattgattt tttctcggca agatttccag aagatagccg tctgtttgat
     4801 tttcactctc aaagatcacc tgatctccga caagcggcgt gattttccga ttccggaaat
     4861 ttcctcttcc tcttgtttga taggtctctc cgtttgcgta tacatagtaa aaaccgctga
     4921 ttgcttttct gatttgtcct ttgataagtg ccacctccgt ttttgtttat tttaccatac
     4981 gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa gtatatggct tgaaaattga
     5041 tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat caatgtcgca ttcccttctg
     5101 atatatttta ttcagcactt attttgaact ttctgaggtg ctagctgatg aacttgtgga
     5161 gctggaagat tcttcttttg aagagttgct tgtgctactg gagctacttg agctgctaga
     5221 actgtctgaa gagtcttttt cagaactaga agacgctgga tctggtcctt tagagtaaat
     5281 tacgttcaca accattcctt tggtaattgt cgttcctgcc cctggtactg tacgaatcac
     5341 tttatcttta tccaccgtgt ttgaatattc ggaagtctca tgcccgatat attctgcacc
     5401 gacactagct aaataacttt gtgcttcgct ttttgtatag ccggagatat cattcaattt
     5461 aggctctgtt ccttggctta cgacaaaatt gatgtaagtt tcttctgcta caacttcacc
     5521 cgaggctgga tcctgactga tgatcgaacc tgatggtaca gtgtcgctat agtcataagt
     5581 agcactaata ttattgatat tgaatccttg tgtaacaagc caattacgaa cagtttcgta
     5641 gttttctcct acatgactac tcaaagtcac tgtttctggt cctttgctga ctgtgaaagt
     5701 aatcgtgtct gttttaggat cgacttctgt atcttctgat ggagattgcg aaatgatcat
     5761 tccgctttct acctctgaac tgaattcctc ttctttttga atcaagtcag cagaaaaccc
     5821 taattctttc aaacttgctt cggcatcttc ataactttta ccactgtaat catctaattt
     5881 gatttttttc gtgccgctac taatatacag agtgacttcg cgtttttgct tgactgtcgt
     5941 tcccgctgcc gggtctgtct tcactacatt accttcttcg attttgtcat ctgcaatttc
     6001 ttctgttttt gttgctactt ctaatttcgc ttttgcgagt gtttctctag cagaagcttc
     6061 agaaagatca gaaacatcag gaacaacaac ttctcctcgc ccgccagaag caaagaatac
     6121 agctgttccg attcctaaaa cagcaagaat agctaatagg atcagccacc attttttctg
     6181 tcctttcttt ttaggatcag gcagaacttc ttcctcctct ggtaaggttt cgttttctgg
     6241 cttgtctttt ggcagaggca tagatttgaa tgaatcaggc attggtgcat cttccgtaag
     6301 gggtgtgata acttttgttt cattatccat cacatgaggc tgccattttt cttcgtttgc
     6361 acgagctggt gataatactg tagaaagatc atcagacatt tcctcggctg acttataacg
     6421 gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt tccagtgctt gtggcacatt
     6481 tggatcaagt gcttttatag atggaagatc atcttgaaaa tgttttaaag caattgttac
     6541 agcagattca ccatcaaacg gtacgctacc cgtcaacatc tcataaagga tgatccctaa
     6601 agcataaata tctgactgct tcgttgccat acttcctctg gcctgctcag gggacagata
     6661 atgaacagat cctagcatcg tattcgtctg cgtgatcgat gtttcagata aagcgatagc
     6721 aatcccgaaa tccgtgattt ttacaacacc ttcattatcg atcagtacgt tttggggttt
     6781 caaatctcgg tggatgattt gatggttatg agctaaagaa atagcagaaa ggatctgctg
     6841 cataatattt actgctgttt cataaggaac aggataatgt gtttggatat atcgtttcaa
     6901 gtccattcct ttgacgtatt ccatcaccag atactgcatg ttgtcttctt caccaacatc
     6961 gtaaacacta acaatgtttg gatgaacgag ttctgttgct gctaaagctt ctctctgaaa
     7021 tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt aagactttaa ctgctacatc
     7081 acgatccaag attaaatcgt gcgccaaaaa tacatttgcc ataccaccgc taccgatatt
     7141 tccagtgatt tgataacggc catttaattt tttgcccaac tcgatcatac actgtcctcc
     7201 ttatattcga taagaagaac ggtgatatta tccgctccgc ctgcttcatt ggcacgatca
     7261 atcagctcag ttactttatg agacaatggg ccttcttgat tgatgatcgt tcctatttct
     7321 tcttcagaca gcatgttcgt caagccatct gaacataaca gcagtcgatc ttttagttgc
     7381 caaagatgag tagaaacatc tacttcgact gttccaggca tgccgacaga acgtgttaaa
     7441 atatttttcc ttggatggtt cactgccatt tcttcgctga tttctcctga tttgactagt
     7501 tcattgacta aagaatgatc ctccgtcaat tgcatgattt tatcttcacg aatcaaataa
     7561 gctctgctat ccccaacatt ggcaatcgtg aaccagtcat ctgacagagc ggccgctacg
     7621 attgttgttc ccataccgtt atattccggc tgttcttgtc cacgctgata aatcagcata
     7681 ttttcttctt gaatcgattg aatgaaccat tgggcgattt tttcatcgtc aacagcttct
     7741 tgtttttccc aagcttcgcc tagattcgtt accgccatct gactggcaat gtctccggca
     7801 cgatgaccac ccatcccgtc tgctaataca gcaagcttga gacctgaacg gctggtaaat
     7861 acactggcat agtcttgatt tgtgttacgt cgtcttccta catctgattg atattcaatc
     7921 tgcatttttt cacctcatta ctttttgcgc aaacaacaaa tgaaaaatcc atctgtgtaa
     7981 aactgatgcg gatacaaagt caacattttc tcttcgattg atgattgtag cataggattg
     8041 gtcaaaacat caatcctctc aaaatcaggg tgtctttcca agaaagcaga aacaacgtct
     8101 tggttttctt cttttaagat cgtacaagtg ctataagtca gaataccgga agactttaaa
     8161 gttggtgcgc agctttctaa aatttccaat tggatttttg gtaaattcgc taattcatcc
     8221 ggctgttttt tataacgaat atctggtttt cgccgaagca gcccaagacc tgaacaaggc
     8281 gcgtctacta aaatccgatc aaactgctct gcttcgaact gtttactgac ttctctagca
     8341 tccatctttt gagcgtagac agcagaagag acacctagac gtgctgcatt atcttcaatg
     8401 agtttgactt tgtgttcgtg tacatctaaa gcgaccactt ctccaccgca tgacggatct
     8461 aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg cacaggcatc taagacatga
     8521 tgttcaggct cgatttgcat agacggcgct acaagcatcg aactttcatc ttggatcgtc
     8581 atgacgccat cgtggaacaa tgagctagaa gctaaatgcc cttttttcgc tactataccg
     8641 tatggactga cttggctttt ttcagctgtg atctcttctt cttgcaaaac agtgatggcc
     8701 tgatctctgg aaagtcggcg tgtatctact cgggcactag catggctagg ctgatatagc
     8761 gataaaccga gcttttcagt ttcttcgtat ccgatttgat ccaccagtcg tttcgtcatc
     8821 catagaggca ggctgatttt gacagaaagt ctttcgatag gatcagcaat gccttgcaga
     8881 tctggtgctc cttgacgctg ataattcctc aagacaccat ttacaaattt ccctgtccca
     8941 ggatttcctt ttacttttgc gatttcgacc gcctcgttta aaatagcatg tgcagggact
     9001 ttatctaaaa attctaattg gtatacagaa agataaagaa gcatcttgac ccagtcatcc
     9061 actttttttg ctttagcgat caatggtgca acgtaaaatt ccaatagtcg ctgatggcta
     9121 atcgtaccat agatcaattc tgtaaataaa cccacatctt tttgatttag ctcgctttgt
     9181 ttgatcatct cattcaacaa aagattcgag taggcgccac ccttgttcac tcgctctaat
     9241 gtcagtaatg ccatatatcg tactgatttt ttcatttttt tagggatttt tttcattatt
     9301 tcacctgctg accaatctga acttgctgac cgcttccgtt aaggaaatca tgtacggcct
     9361 gttttccttt accagctggc tgtaattcat tgatggctaa gacggtctgt ttaccgcaag
     9421 caatccatag attttttttg tctttccgga tgatcgtacc aggtgctttg tctgtagttt
     9481 ctgccaatac ttcaaccgat aataatttcc agcgtatgcc ctcataggtc gtgaatgcaa
     9541 ttggccaagg acgcattccg cgtacttggt tgtcaatttc ttctgcagtt ttatgccaat
     9601 cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt tgcttctgac tcgtcttgag
     9661 gtactggttt ttgtccatcc aaaatattgg gcaatgtttc tagcaatagc tttcttccta
     9721 aaaggctaag cttttcaaac atcgtcccta catcatcttg tttagtgatt ggtatgcttt
     9781 cttgcgcata gattccgcct gcatccattt ttttgatcat ttccatgatc gttacaccag
     9841 tttctttctc cccattcatg atggcataat gaacaggcgc acctccccga tatttaggaa
     9901 gtaaagaagc atggacattg attgccccta attttggtac ttgcaataac ttttcaggca
     9961 agaattggcc aaatgccgca gtgacgatca catcaggcac caattcaatg atttcttcca
    10021 tttctggcga accggagatt ttctctggct gcagcacacg gataccgtgc ttcagagccg
    10081 ctttttttac cggtgtcggc gtaattactc gtttccgccc aactggacgg tcgggctgtg
    10141 ttaccactgc ctggatctcg taacctgatt ctatcagccc ttcaagaatc ggcacagaaa
    10201 aagcaggtgt gcccataaat actagcttag tcatcaagat gttcctccat atattcttca
    10261 agttcttctt ccgggatctg ctgtatcatt ttctcaatga acaagatacc gtctaagtga
    10321 tcgatttcat gctggaaggc acgggctaaa tagccaaaag ccgtcacttc tatctcttct
    10381 ccgtctcgat cataataacg cacggtcact tcatctgctc gcttgaccgt tccatagaca
    10441 tgcggaatac tgagacaacc ttctacgtca aggctttcac cttttgcttc gatgatttct
    10501 gggttaatca gttcaaattt ctcgccttca tctacttcga tcacagcaat tctcttgttt
    10561 tgtcctactt gaggagcagc gatccctatt ccgtcattcg caatcattgt ctcatataaa
    10621 ttgtctaata aagcgattgt ctcgtccgtg attacatcga ttggctgagc agttcttttt
    10681 aatttatcat tagggtggat caatatagga tagcgcataa tttggccctt tcttaaataa
    10741 agtttaatgg ttcgttgtca attgccacaa aaagaccttg tcgctgttct ttctggcttc
    10801 cattcaaaat ttcatccaaa acatgaggaa gattcggttc atgtttatat ttgatgatta
    10861 gttgataata atatcggttt ttcactctca aaatcatgct gggcgttggc cctaatagca
    10921 agctttgagg gcttaacact tcttttagct gattggcaat ctgaaagatt ttctttgccg
    10981 ctacttgttc ttcaggatgg ctggcggtta ttttcacagt aaagtaataa ggaggatagc
    11041 ctccacgatg ccgtacatgc atttcctgct gataaaactg ctcataattt tgttttttag
    11101 ccaattctat ggcgtaatgt tgaggattga atgtttggat cacgacttcg cctgcttttt
    11161 ccgctcgccc tgctcgtcct gaaacttggg taagcaattg gaatgtatgc tcactagaac
    11221 gaaaatccgg taaattcagg gaggtatcag cattcaacac cccaactaat gtcacttctg
    11281 gaaagtccaa acctttagca atcatttgtg tccctaataa tatatctgct tcttttgcac
    11341 cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg agtggtatct acatccatac
    11401 gcaaaatcct agcttgcgga taaagttctc ggagttcctc ttctactttt tgagtacctg
    11461 ttccatagta gcggatttta ttgccgccac aattaggaca gcgattggga ataggttctt
    11521 catgtccgca ataatggcag cgcattgaac gtgtatccat atgcagtgta agagagatat
    11581 cgcaattagg acatggaaga acaaatccac agtccctaca catcacaaaa gacgaatatc
    11641 ctcgacgatt gagcagtagg acagtttgtt cttttttgtc taatcgattt tgtatttttt
    11701 cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg aaactcttca cgcatatcta
    11761 cgatctcaat agctggaagc tgtgcagctg ctttggctcg gtggtttaac tgcaataact
    11821 gataaacgcc tttttgagct cgtgctcttg attctaaaga gggcgtcgcg cttcctagaa
    11881 ctattgggca atgatgatat ttactgcgcc aaattgctag atctctcgca tgataacgcg
    11941 gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc gataatgatc agcccgatat
    12001 ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac tacttgcgct tcttcccgct
    12061 caatcttgcg ccattcatca tatttttcac cttgggaaag tccgctatgt aagaccgcaa
    12121 ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg cggagtcaat gcgatttcag
    12181 gcactaacat aatagccgtt ttattttctg acaaaacatc agcgattgcc tgaagatata
    12241 cttctgtttt cccgctgcca gtgattccct ccagcaaaaa gacctgatct ttttgctgct
    12301 gacctgctgt gatgatttgt tcgacagcat ttttttgttc tgcattcagt tccaatgccg
    12361 tcgtttggtc aaatactcgg tctttataag ggtcgcgata acgttctgat tctataaact
    12421 ccagccagcc cttttctttt ccttgattca agacagcagt actgattcct ttatttttga
    12481 aataagcaat tggttcttct tggctcagtt cagataaaca caaaagaagt tctttttgtt
    12541 tcttggcatt ttgccgtaat tttgcccatt cttcttcgat ttgcttttca gttaacaaag
    12601 aacggaccaa acgaatcgtt tttactttat ttcgtgtatg gacttcatat ttgatatcta
    12661 ctaattgctc ttggcgccag cgaatcaatt gagaaaggct gccttcttct tctgcttcct
    12721 tccaggaaat cacttctcgc tgatcaaaca atacctgaac ttctggtgtg tctgccaaag
    12781 gatagatcaa tttatcatat tctgctttca tcacactcgg aagcatcgtt tgtagacaag
    12841 tgattttgaa tgcgtaagta atttttttca tataatcagc taatgaaagt aattcttcat
    12901 tgaccaccgg agcaaggtct agcaaacgaa taactgcctt caaagatgga ttagctgatt
    12961 cttctgattt ttggatagcc ataacaaatc cttggacatg acgattgcca ttaccaaaag
    13021 ggacttctac tcgcatcccg acttgcaaag ctgtttccat ttcagaggga accaaataag
    13081 tgaatggctg atcggtctgc atagtaggta cgtccacaat gacttcagct gttaatgtca
    13141 tggttcgttt cctccttttt ttgtcttttc ttatcaagcg acaaaaagtt ccttgcctat
    13201 tttactagag atcgatggat ttgtcttatc ttctgccaaa tcctaacaaa aaattaacag
    13261 cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca cagccttttc taccagacaa
    13321 taagtggcga atgtttgaaa aggaaacagc tgggtttgct tttcttttta gatgttcgct
    13381 ctatttttct ttattacatt ttttgctctt cacggatacg tgattccaat tcgtgttgtt
    13441 cacgctcttt ttgtgcttga cgttcctctt cttccatctt caaacgttca cgttttaatt
    13501 caggatgtgg atcattgact acatttcctg catcgatttc ttctaaagct tgaccgacgc
    13561 tttttactga atcaaaagaa tctagagtag gctgtgcact tgcatccaat tcatgcgcgc
    13621 gtttacttgc taaaatcact agagaatatt ttgaatttac acgatctaat agtgagtcaa
    13681 ttgaaggttt taacatcata agactacatc tcctttaaca ttttaatata tttaccgatt
    13741 acgcgatcta ctcgaaaatg ttcactggca ataatgtttt tgattcgttc aactgctagc
    13801 ggcacttcat catttacaac agcatagtcg taaagagcca tcatttcgat ttcttctttt
    13861 gcaactttca ttcgtttttc gattacttca tctgcatctg ttcctcggcc tacgatccgt
    13921 gattttaatt ccgctaaatc tggcggagtc aaaaagatga atacaccatc gggcactttt
    13981 tctttgactt gttttgctcc ttggacttca atctccaaga aaacatcttt tccttcatcc
    14041 aatgtcttgt tgacatatga caaaggagtt ccataataat tcccgacata ttctgcgtat
    14101 tccagcatct ctccagcttc gatcattgat tcaaactctt ctttgcttcg gaagaaataa
    14161 tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag tcattgagat cgaatactga
    14221 aaatcattat cctcactctc aaaaattgct tttcgtactg tgccttttcc tacaccggat
    14281 ggaccagaca acacgattaa taatccacgc tctgacatga cgacgtcctt tcaactagtc
    14341 tttttgtact tatcgtttat tttgcacttt ttttctgcaa ctttcaaggt tatctttaat
    14401 tatagctaga aattggtaaa aaagctaccc tgtaccaaga ggtcgtaaaa aagctgtcct
    14461 gcatcaagta ataagaacag ccaaacataa acagcttctc atgtttttcg tttggttggg
    14521 gttattatcg cagatgcaag cttttgaacg ccgtgtataa gaggtcgtga agaagccgat
    14581 tagtttcgag tatgaagaaa aattttgtga aaatagtttc tcacattttt gcataaattt
    14641 ggcttaatac cgagaaaatg gtttttgaac atcacttatt caaaataaaa aaagattgtg
    14701 acacgaattt tgtcacaacc tttaaatgta ttcctagagc aaagaagcta agcgaagaaa
    14761 tccaattctg tttatttatt gtatttcttt gttcagcatc cttgctaaaa gcccatattg
    14821 gacaaagtat tcactacacc catgatctca tcttttcgag aacgtgcttt ttctgtcgga
    14881 tcttctacct cttctgtgtc aattcgaaca ataaatttcc gattcatcgt cacgtaagca
    14941 ccgtcgttga tcaaaataaa cagttcgttc tcattatagt ctgcacaggc atctttccat
    15001 acgtcaaagc cgtttttttc agaagggata tcccgtttaa ccacagtttt tccacttaat
    15061 tcgtggaacg ttacgtcata atatttcatt atctctacct ctttcgtcat tcaatatttt
    15121 gtacttgttc tctgattttt tctaagatcg tcttagactg gacgacttga tttttaattt
    15181 cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg attcatttct tggatcagga
    15241 aatctaattc ccgacctaca ggacattgtg cttcgataag ttcatctagt ttttgtacat
    15301 ggatcgccag acgatccagt tcctcgtgaa tatctccgcg ttccaacaag atggctagct
    15361 ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa ttcctccagc tttttttgat
    15421 accttatttg aaattccttt tcatagattt ctacaaaact atcgagtttt tttagtactt
    15481 ctttgaattc ttcagtattc tttttcaaca ctaaagctaa agcttgacct tctttttctc
    15541 gactcgcctc aatagaatca agtgcgcttt tcaatgtatc tagagctaac tgtgccaatt
    15601 cttccatgct ctcttccggt ttttcttcta ccgttacgaa gttttcgtcc agcacgaagc
    15661 gttccgccat ccgactaatt gacacttgat tttgactacc gtatcgttgt tgaattccgt
    15721 ctgtcaattg agtgataagt gaatcaatca attcccagtt gatgacgatc tgtttttcgt
    15781 ttttgccaat ataagctaga ttcacaaata cttcgattcg accgcggctt aactgctgtt
    15841 taataagctg acgaaaatcg ttttcaacaa agttcaggat tttaggactt cgtatttgaa
    15901 catctagaaa acgctggttg acacttttta tttcaatatc taactgatac gcttttgttt
    15961 cgcggatggc tttgccaaaa ccagtcatac ttttcattcg acagggtcct tcctcattac
    16021 atattttcta ttgctttttg cagttgttca aactcttcgt tcgtcaatgt caggcctttc
    16081 cccatttttt catggtctgg cgcccaatct cgaagatcga attttggcgg gcgaccattc
    16141 cagctgacta ggttcagttc tttacgccac cccttgttgt tttctgacaa tatagcgatt
    16201 tcttctacga tttcatatga aaattcttgt gccatggttg ttcctccttg agataatata
    16261 aatataggat cagtaatgtg tcaactggtt gcttaagcaa ttcatgctgc aaacgatcct
    16321 tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt cgtgcgctca ttttgtattt
    16381 tttgctggta ttttcgatac cttcgccatt cttcagtact taattgacca atttgtgcca
    16441 ataaatcatc cggcatactt tttatatctt gttggcagat catttgaatc atcatgtcta
    16501 cttgatagat ttcattgggc agtaaaaaat gtttttttgc taaaagttct agcatcgcca
    16561 atccgcatgt ctctggtacg accctgttct tatataaatt acgcagtttt tgccaaaatt
    16621 ttatgctttt gaaaaaagat aattctaact gaatttcttt tgttaagtaa gacaaaaatc
    16681 ctcgccagta tgttcttgca ggtttaacag aatgaaatgt ataccagttc ccatagtaat
    16741 agcctaatcg ttttttcatt tccagatagc ctaattgtaa atttaattgg ttcctttggc
    16801 tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg acatttccaa tggataaaaa
    16861 tgtctggcat acgaattttt ttcgctgggc ctggcccttg cacatctacg acaaatgcct
    16921 ctgttgctcc tttgttaatc gcaatatcaa taggtatttt gttgcgatac ccaccgtctg
    16981 catatttatt ttttccgatc tttcgataag ccatagcggg ataaaatgaa gcagaggcga
    17041 gtatccaatc tggtatttct tctgtttttg tctgttggat atcagtcact acttccgcta
    17101 gttctggcag tttagtagac accgtatata aacgtatttt gggattatgt ttgagtaagg
    17161 acaaatccaa attgtctttg accatctttt ccaatggagc agaagaaacc ccgccttcaa
    17221 ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa gcgttcacga aggtcttcaa
    17281 ctgcagccat ttctggtaac gcaagcactt gtcttgtcga cagctttttc cataaattca
    17341 cagctttttt catatctcct tgtaaaatca atgcgccgtt caacgcacct accgaggtac
    17401 ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc ctgccacaca ccaatctgat
    17461 aagcaccgtg cgctcctcct ccacctaaaa caagtgctgt atgatagacc agctttttat
    17521 ttaattgtgt ggttccttga tagccgtttt tatgaaaaat acgcaaccat tcttctgata
    17581 gaggagattg aaataaaaaa caacagtttt ctttaataaa aaaacgagcg caagcttcca
    17641 tataccgaaa aaattttttc cattcaaaag tttcaccttc aatcaacaga ttattaaaaa
    17701 taacatctcc tctactttta gcaatagtaa aatagacggt atattttttc gatactgctt
    17761 taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc tctactttct tgatgacttt
    17821 gaaaaaatgg aaggggttgt tttgcttgct ggataggcga atacagcgac attttctgtg
    17881 aaaagtaaac tttttttatc tccatcgcgc tctccttttt cttcaagtat accagtacgt
    17941 cataaaaagg tcatgagtct cgtattcgac tttgtagatt ccataagaaa acaattcata
    18001 aaacctcctt taaataaaaa aatacaaaaa tcacaaaaat taattgaaaa tgagagtgtt
    18061 ttcaatgaaa acgtgatttt gtaatggaat tttaaaataa aagaaacaag cttttttaat
    18121 gcaaatgttg taaaataaaa gagattaaga ggaaataaga ggaggaacat gatgactgag
    18181 tcagtttatg tacatattga taccacaagt aatgctgttt taacaaaagg gctgactgct
    18241 actgactttg caaacagtat cgtgcatttt cctcaaaact tgctcttgct tgatccatct
    18301 gcttctgctg gagaatatga atcccataca gggctgaaag taatccgtgg tacagaaaat
    18361 attcagcgtt tcttttcctc ttctcgtaac aggcgtggat tcgatgaatt gaagtggatc
    18421 gactttactg atttaacgat gctgaaagaa ttgacacctt tagaaatttc agaattatta
    18481 tattttggtc atatgaaaac acatcttcat tcgccgtttt tttataaact acagaataat
    18541 tttgtatact tcgatcttaa tgatcagctg aatcggattt attatcgcta tttagatgaa
    18601 ttttatagga tttttgctag taaattaagt tcgatcttgt cagataagat caatgtaaaa
    18661 aaatcttttt tcaaaaaaga aacacctgtt gaaaaaatca gtgtggaatt attgaaagaa
    18721 atgcgtaatt tgatgcagga agggatcatt ttttcttttg atcaagttgg tttgatcgat
    18781 ggtaaatatc gtatcccaat ctatgtgatt gaagattata tttggaaatt aaaaggcacc
    18841 cggtatcgag aagaggaaat catcgctact ttaatctact ctgtcaccga tcgacactgg
    18901 caattggtag aagatgccga tctttcttat ctgccattga agtagatcta tttcttatca
    18961 aaaaataaaa aggaaacaag aaactaggac atgattctgt ctcaagtttc ttgcttcttt
    19021 tttatttaca gctcctgtcg tttatctggc acaaaaatcg aaggagtcgt ttgttggatc
    19081 cctaataaaa cgatgatcgt tacaatagct gtactcaacc cgctggcacc attcattacg
    19141 aaagaaaaga gccaaggatt catcccccat agtgcatacg ctccccagaa aaccactcct
    19201 gcaataaaat gccaaaaata tctggaaagt gcgccaatca atgcaccaaa cacgatgctt
    19261 tttctaattt gtttcgtatt atcgtctaca tagccttttt ggacatttga agccatgagc
    19321 cctgctgcgc ctgcaaaagt ataggcaatc gggtactcaa tcaacacttg aacgacactt
    19381 aaataataaa cttgtcccaa cggaaaatgt aggatgcccc aaatgaaacc tgccagcatc
    19441 cctgcctttg tccctcttct taacgcaaaa agagtcaatg gaatctgccc taatgaaata
    19501 gaaaaactgc tgccgatctg gatcggaatc aacgacaaca ccatcgctaa cgctgcaaca
    19561 atcgttcctt ctacccaaac tctatttttt cccataaaaa aacttcctcc tttgaataaa
    19621 aaagtcacaa aggaggaagc agctttcgaa aatagtcaaa cagctgttta ttcacaattc
    19681 ctacgctcgc ataaccgata caggtacgaa gggtttagaa atagattcaa tctcagccca
    19741 attggactcc cctttgtgag tttatttatt atttattttt tgtttcaaac aatggactaa
    19801 ctggtttgtt ttcatggata cgtttgattg catctcccat tagtccgcca acacttactt
    19861 catcgatttt gtcgatttta cgatcttctg gaagatagat cgagtctgta acaactagac
    19921 gctcgatagc tgaatcttct attctttgta aagcagggcc ggataatact ggatgtgtac
    19981 atgaagcata cacactagtt gcaccagctt ctttcaaagc attggcagcc aatgtgatcg
    20041 tacctgctgt atcgatcata tcatcgatca gtacacaagt tttgcctttg acttgtccaa
    20101 tgatattcat tacttctgca acatttgctt ttggacggcg tttgtctatg atcgcaatcg
    20161 gagatttcaa aaattccgct aatttacgtg cccgtgtgac accaccatgg tctggtgaaa
    20221 caacgacaac atcatcgcct ttgattcctt tttctaagaa ataattggca ataagtggcg
    20281 cacccatcaa atgatcgact ggaatatcaa agaaaccttg gatttggact gcatgaaggt
    20341 ccaaagtcag cattcttgtt gcaccagctt tttgaatcat gtttgctact aatttcgctg
    20401 tgattggttc ccgtgcacgt gctttacggt cttgacgcgc atagccataa taaggcatga
    20461 ctacgttgat tgtttttgca cttgctcgtt tcagtgcgtc aatcatgatc agcaactcca
    20521 tcaagttatc attgactggg ctgctcgttg attgaataac atagacatgc gatccacgaa
    20581 tactttcttc aatgtttact tgaatttctc catcactaaa ttgagtaaca gatgattttc
    20641 ctaactcgac gccgactgct gctgcaattt tctctgctaa tgggcgattg gaatttaacg
    20701 caaaaatttt taatcttggg tcaaagtaat gttttgacat ggggacctcc actttctttt
    20761 tcacaactct agaattctac ctaataaata gtagtcatat tttttaaata aatcaagtga
    20821 ttttgcaaaa gtcttagttt agataaggga gtttttttgc atatccttct ttattgactt
    20881 gtcttgctct cgcaattgcc agcgcatatt ctggaatatt gtctgtaatt gttgacccgg
    20941 cagcaatcga actgttttta gctacctcga caggtccgat gatattcgtg ctagaaccaa
    21001 taaagctgtg gtcgccgaca gtcgtatgat gtttgttctt tccatcatag ttgacgaaga
    21061 caacaccaca tccgacattg atttcttctc ccagcgtagc atcaccaaca taagtcaagt
    21121 gaccgacttt agttcctttg ccgatttgag catttttcac ttccacaaag tttccgatat
    21181 ggacgttttc gccaatttca gcttttggtc gtaaatgtgc ataaggtccc acatctgcat
    21241 gtttcttgac atggctgctt tcaatgactg agttttcaat gacgacgtga tcctcaatcc
    21301 tactgtctac gatttttgaa tgtgacccga tgacacaatc agaaccaatg actgtatttc
    21361 cttggatttg tacacctgct tcgatcaatg tgtctggacc gatttctacg cctgcatcga
    21421 tgtatgtcgt tgctgaatcg acaaagctta cgccattgac catatgcatc tggttaattc
    21481 gctttctcat gatctcattt gctttagcta acgcgatccg atcattgact cccattgatt
    21541 cttcaaaatc atccgtttga taagcagcaa ctgtatggcc ttcttctttt aagatttcga
    21601 tgatatctgt tagatagtat tcaccttgag cattgtttgt tccaactttg cttaacgcat
    21661 cgaacaaagc ttcgttgtca aaacagtaag ttcctgtatt gatttcttgt acaagagctt
    21721 cttctggtgt tgcatctttt tgttccacga ttttttctac gatcccaata tggtcacgga
    21781 tgattcgtcc atatcctgtt gggttttctg cttgtgccgt caagatcgtt gcacttgcat
    21841 tttttccttg gtgatattca aaaagattgt tcagcgtttc agtagtcaat aatggtgtat
    21901 cgccactgat aactaacgtt gttccttttt ttccttccaa gaaagaagca gcttgcaaaa
    21961 cagcatgtcc tgttcccaat tgttctgctt gtaacgcata tttactgcgt tctcctagtt
    22021 gtgctttaac ttgttctgct ccgtgtccaa cgatcgtaat cacttcatct ggtttcgttt
    22081 cactgacccg attaatgata tgttctacca taggtttacc agaaacggga tgtaaaactt
    22141 tatataattt tgacttcatg cgggtacctt tccctgccgc caaaataatt gcataacgtg
    22201 cgtccaactg cgtcactcct aaatatagtt tgctgatttc agtttatccc agtataccca
    22261 ttttttcaac aaaacaagtg attttctttt atcccagcgt tttctggata aaaaaataga
    22321 tagaccataa cgggcctatc tatttggtaa ccggtgctct cctgccaact cctcggtcac
    22381 aagtcacact actcataaaa catgaaaagc tgttttttga aatcgtttct tgtgacttag
    22441 agttaaacgg taggcttaca acctcttcat taaacgtttt tcaatcagct gaccttcggt
    22501 attagctcaa taaacggcaa aatatgagga gcaatttttc cgtttattga gctaatactc
    22561 aggacaggac gctgatttca caacctctag ataaacggtg ttcaaaagcc agtttctcgg
    22621 tattaagcca aatttatgaa aaaatgtgga aagccatttt cacaaaattt ttcttactac
    22681 tcgaaactaa gcggcttttt cacaacctct tattctgaaa aataatttcc tggcacaacc
    22741 gtaatatttt tcgtttgagt atctacatct ttgacataaa gcagtgacgt ataatcatcg
    22801 atggcgcgat gaccgttgaa ttttgattcg gcaaatacag tgatgccaac aagttctgat
    22861 tcgaattctt caatcaagct cttcattccg ttgactgttc cgccgccttt catgaaatca
    22921 tccactacaa ggacacgtga accacgtttc aaacttctct tagacagttc catcttttca
    22981 attctttcag aagatccaga cacatagttg acacttactg ttgatccttc tgttattttt
    23041 gagtcccggc gtacaataac gaatggcgca ttcagataat aggacacagc ttgggcaata
    23101 ggtacgcctt ttgtggctac agtcattaca gcatcgattt tttcaccaag gtatttagaa
    23161 gcaatgattc ttccaacttg acgcagcagt tcaggttctc ctaacaaatc agagagataa
    23221 acatatcctc ctggaagcag acggtcttgt tctgatagac gttctgctag agattcaata
    23281 tattttttcg cctcttcata tgaaatttca gggatgaaca atacaccacc tgcagcaccc
    23341 gggattgttt ccaaggttcc ataccctcgt tctttaaatg ttttcttgat gattcccaaa
    23401 tcttcactga tagaagattt agcagattca taacgttcgg caaagcttgt taaggaaatc
    23461 aattcatgcg ggtgattcaa taaatactgt gtcatgtcaa ctagacgttc actgcgacgt
    23521 actttcacct attccaactc cttttaagaa attttatcgt tttacattat ataagttttt
    23581 cttcaaaaat tcgagcattt tatgaaaaaa taagaaaata gttcggtatt tattaaaata
    23641 aaaagaagcc tataaaaaat ttttcgtttt tataaggctt cttttcttta taatttagtt
    23701 attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca ggaacaaagc
    23761 aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg agctagttag
    23821 tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga cattgaagct
    23881 tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata tcaaaagtgc
    23941 accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga acatcatcga
    24001 catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag tcagaacata
    24061 catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga ttcctaaaaa
    24121 gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt ggatacttgt
    24181 tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac caccagccat
    24241 taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat actccaatat
    24301 cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa
    24361 tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca gcgattgtct
    24421 tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag
    24481 aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc ctttctcacc
    24541 agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt gatcattaga
    24601 actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc atttctggat
    24661 tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc agtagcaaaa
    24721 atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat tcgccgattt
    24781 cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg atctttttca
    24841 gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa ttcgatcacc
    24901 gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc gatacttaat
    24961 ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg tttcagtaat
    25021 cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt taaaatgaca
    25081 aattcacccg catctacatg ataagagaca tcttctagta caggttcatc atcatagtaa
    25141 aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta aaacttaatt
    25201 gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat tttcgcctgc
    25261 tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct tcactcctgt
    25321 ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat aaatcacttt
    25381 gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg tgggttcctg
    25441 atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg ctaaataagc
    25501 aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct catcggagta
    25561 tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg taaaagtttc
    25621 ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt cttttatagc
    25681 taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat gcccttcttc
    25741 tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt tagctgcttc
    25801 gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa aaaattccat
    25861 gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt cttttgcact
    25921 tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt ttccttcatc
    25981 ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc
    26041 ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta aagcacctat
    26101 cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa tgaacaaatc
    26161 ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag
    26221 aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa aatggatgag
    26281 ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc attttaggat
    26341 tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt cgtactaaat
    26401 aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt gaataatgtt
    26461 gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct gctccgtact
    26521 tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt atatcttcta
    26581 aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg gcttcagcaa
    26641 gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac actgtacgtg
    26701 tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata gaaggtaata
    26761 cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca cagtaaggaa
    26821 catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag cccagattcc
    26881 ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg cctaatccag
    26941 ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata ttgtatttat
    27001 tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt aaaaatgctt
    27061 tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga tgatctgcca
    27121 agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt ctatataata
    27181 catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc ctctcaccca
    27241 ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta tagcttatgt
    27301 ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt ctcttcataa
    27361 tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc aaactcggaa
    27421 catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat ccgaaattac
    27481 ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt aatttctttt
    27541 cataagcaga ctggaaggaa tagaaaaaat taccataatt atatacgata tgcatgactt
    27601 tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct aagcctagat
    27661 tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac agatagtcag
    27721 ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg
    27781 tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca ataccgacac
    27841 cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac
    27901 cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc ggcagttgat
    27961 cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt tgctgactca
    28021 gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact ttcgtgaagt
    28081 gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa aacgcaagct
    28141 gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc atcattttag
    28201 gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga taaccaaaaa
    28261 cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac aagcgaagct
    28321 gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt
    28381 actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc actaaagcta
    28441 gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa ggaaacaaaa
    28501 taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg
    28561 cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct ccccaagaat
    28621 aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac agaatcaaga
    28681 catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa ataccaaaag
    28741 atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt gctctagcaa
    28801 ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa ttgaatgaga
    28861 acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt gttgatagaa
    28921 gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc gcaacctttt
    28981 ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct gtatgtgtga
    29041 tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga taatataata
    29101 gccaaactac tgctacatct tgccgaacag acaattgact cattcctaag atcatcaaaa
    29161 cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat gtagcaatca
    29221 tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg ccgatgatca
    29281 aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga ccaagcaagc
    29341 cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa aagaaaatga
    29401 ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa atactttctg
    29461 actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa ccagtcagca
    29521 taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc tgctttccat
    29581 aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga ttgttgatcg
    29641 tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt tttccttgtt
    29701 tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca cccagcagtc
    29761 cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt tgccaaatat
    29821 cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag actaacacgg
    29881 agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc agcatcaagt
    29941 atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc ataccccaat
    30001 gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc gatgatagaa
    30061 aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt tatcggcaca
    30121 gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa
    30181 gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc tgaaactggc
    30241 gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt tcttttagcc
    30301 gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag ccagaaagtc
    30361 ttattcattt ctattgcttc aggaaaatca ttaatagatt tagaaacagc gcttgaagat
    30421 cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt
    30481 tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc tacgatgatt
    30541 tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac attcattggc
    30601 cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg acggactggt
    30661 gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc aattatgggt
    30721 tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga ccaagtttct
    30781 tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg attcatccca
    30841 gcaatcatca aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa
    30901 taatgatagc aatgtttttt cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg
    30961 gctcttcttt tgtatttttt attatttagc ataatcagtt gcacgagttt cccgaataac
    31021 gattactttg atgtgtccag gataatccag ttcttcttcg attcgtttac gaatatcacg
    31081 tactaaacga acagcgtcta aatccgagat ttcttctggc ttgaccatta cccgaacttc
    31141 gcgtccagct tgaactgcgt aacttgattc tacaccggcg aagctgttcg aaatattttc
    31201 cagacttctt aaacgacgga tgtagttttc taatgattca cttcgcgcac caggtcttgc
    31261 tgctgagagc gcatctgctg cagctacaag gacagagata acagaagtag cttccacatc
    31321 tccatgatga gaagcgatcg cgttgattac tactgggttt tctttgtact tggcagctaa
    31381 ttccgcaccg atctcaacgt gagatccttc aatctcatgg tcaagtgctt tcccaatatc
    31441 atggagcaac ccggcacgtt ttgctaattg gatatcttcg ccaagctctg ctgctaagat
    31501 accagctaat tttgcaacct cgaccgaatg cttcaagacg ttttgtccat aactagttct
    31561 gaatcgcaga cgtccgatga tcttgattag atctggatga agtgtatgag caccgacttc
    31621 aaacgctgcc tcttccccgt attcgcgaat acgttcatcc atttctttac gagatttctc
    31681 gaccatttct tcgatacgcg ctggatggat tcgtccatct tgtatcagtt tttccaaggt
    31741 catccgtgcg atttctcttc ggattggatc gaatccggaa agaacaactg cttccggtgt
    31801 atcatcgatg attaaatcga taccagtcag cgtctcaagc gttcgaatat tacgaccttc
    31861 tcgtccaatg atacgacctt tcatttcatc attcggaagg gtaacgactg aaacagtcgt
    31921 ttctgaaact tgatctgctg cgcagcgttg aatcgctaat gataataagt ttttcgcttt
    31981 gcgatctgct tcttctttgg cacgctgttc agattctttt accattagag ttaactcatg
    32041 attcagctct tcttcagtcg atttcatgat gacatctcgt gcgtcctctt ttgataaacc
    32101 agcaattttt tctagttctt tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg
    32161 ctcttcaatt aattgctgtt ttgaactaag tgcttcttct ttactttcaa gtgagttctc
    32221 gcgtttttct aacgagtcat ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt
    32281 ctcttgagat tttaattcta acttactttc tctcagctca ctttcaactt cagaacggta
    32341 tttctgattt tcttccttag cttctaacaa tgcttctttt tttagagttt ctgcttcttt
    32401 ttttgcacta tcgatgattc cagcagctga tgattgtgca ccagctattt ctttctcgtg
    32461 acgtgacttt gcgaccataa accctaaacc aagaccgaca attaaaccga tgatagcgag
    32521 gagaatgttg aataccataa aatccacctc catactatct ttttcatttt tatcatctat
    32581 acagcttcta tgataaatta ttctaaactc gtcaatatgc atcttcgcat atatgtgtac
    32641 aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac
    32701 aaagaaacgg ctttctactt attcattaaa cgatggtttt taaaataaat gacactagtt
    32761 tcatataggt aaacctttaa attgagaaac ttttctatta aattattctt tgtagatgaa
    32821 cagatatttt tattttatgt acccactcgt ataattaaat aactcacgat ttctttattc
    32881 aataaaaaaa cggcaggtca aggagagtac ctactcttca gaccagccgt ttttgtttat
    32941 cataattttt attcttctac taaaggcaat tcttcttgtc ctttttcatc ttctgggaca
    33001 gcgacttctt caccaatacc ataagctgct ctaactagag cagaaacttc tgccatcatt
    33061 tcaggatgat tagccatata gattttagcg ttttcccgtc cttgaccaat ccgatcttct
    33121 ttataagaat accaagcacc gcttttatca acaatgtctt tttctactgc catgtctaga
    33181 agttctcctt cttgtgagat ccctaaacca tacatgacat ctacttcagc gattttgaaa
    33241 ggaggtgcta ctttgttttt tacgactttg atctttgtac ggttaccaac aatatctgta
    33301 ccttgtttca attgttctgc acgacgtact tccaaacgga tcgttgcgta gaatttcaat
    33361 gcgcgtcctc caggagtgat ttctggattc gcaaacatca cgcctacttt ttcacggatt
    33421 tggttgatga aaatagcgat tgtttttgtt ttgttgatcg aaccagagag tttacgcaat
    33481 gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt cacccatttc gccgtcgatt
    33541 tctgctcgtg gaactaaagc agcaactgaa tcgactacta cgatatctac agccccactt
    33601 gatactaaag catcagcgat ctctagacct tgttctcctg tgtcaggctg tgaaagaagt
    33661 agttcatcga tattcacacc taatttttgt gcatattgcg gatctaacgc atgctcagca
    33721 tcaatgaaag cggccgttcc gccatttttt tgtacttctg caatagcgtg tagtgcaact
    33781 gttgttttac ctgaactttc aggtccatat acttcgatga tacgtccacg ggggtaaccg
    33841 ccgacaccta aagcgacatc taacgcaaga gagccacttg gaattgtaga gatttgttgg
    33901 tcgatttttt cgcccaattt cataatagag cctttaccat agctcttttc aatttttttc
    33961 agtgcagcat ctaaggctgc tttacgatca tctgccaata cgataatcct ccttatattt
    34021 acaaatcaga gcaacatttc attatcctta tcatatcttt tttagtgaaa aaaagcaaga
    34081 aaaaaccgaa caaatattcg catttatcat tttcttaaaa gctgttttct caaaagatca
    34141 aatccttgca taaccgcatt atatctgata gcagcacgat ctctggcaaa gtgatatttt
    34201 tccgaaacca cgccgtcttt agatgaaata gcgatccata ctgttcctgc agattgacct
    34261 tctaacgtgt ccggtccagc cactccggtg aaagctacac tgtaatctgt tcttgctatc
    34321 tcacgtgctt gaatggccat ctgctccaca cattctttgc tgactgttcc atattctgct
    34381 aatagctccg gatcaatctc cagaaaacca gcttttgttt ggagcgagta ggtaacgaat
    34441 ccgcctggaa atacttcaga tacaccagca atgtcgccta atgcggcttg gaaggcgccg
    34501 gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt ctttcagtag ttccactacg
    34561 acttttgcta gcgaattatc atcgccgtat ccataaaaat attcccctac tcgttcttgg
    34621 attttttctt ctaatgctaa aagagcttga ttccctgcct gaacatcatt tgttttcact
    34681 gtcagtcgta atgtcacttc atttggtttg gcatatggtg cgatcgttgg attgatctgt
    34741 gtctcaatca gatcttttag ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc
    34801 aaaactttag aaattagttt ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact
    34861 tgttccacaa acattggttt caactcatta ggcggaccgg gaagaaggat atacgcatgt
    34921 gtatctgttt gataaaaagt ccctaaagca agccctgtac gatttggtag aggaaccccg
    34981 ccttcgataa tctgggactg ttgcagattg tttttggtca tttttcgatg ggttgtttca
    35041 aaatatgcca ataatttttt gtatccttcc gtattttgta tgagagactt accgaggtga
    35101 gccgcagttg cttcttttgt caggtcatct tctgtcggtc ctaatccgcc acataagagt
    35161 atgagttcac ttcttgtttc agctagagca atcaactctt ctaaacgctg ctgattatct
    35221 cctacaactg tttgataata aacttcgata cctagatcag ctagctgttc tgacaagaaa
    35281 gtggcattcg tattcacgac ttgtcctagt aaaagttctg ttccgacagc tattatttct
    35341 gctttcatta cttcccgctc ccttatatag tatacgctgt atttttatat ttttttattt
    35401 aaaaagaggg caaggagctg tgacaaaaat cttgtcacag ctccttgttc caaataaaca
    35461 gtgttctcct gccaaaacct cggtcataag tcacaatata cataaaacat gaaaagctgt
    35521 tttttgaaat tatcccttgt gactcagagt tagacggcag gctcacaacc tctccactaa
    35581 acgatactta cattgatcct ttgaacacac cgctgttttt gatgaagtaa tccacacctg
    35641 aatagatagt aaagatcaga caagcataaa gcatgatttg atctactgga ataccgatca
    35701 ggctgaatgg gatattattg ataaacagca agataatagc aaccatttgt gtagccgtct
    35761 tgacttttcc cggccaagct gcagccatga cttctccatg ttcgacaagt agtaaacgca
    35821 agcctgtgac ggctaattca cgacagacaa tgatcgctac tacccaacca ggggcctttc
    35881 cttggtcaac caacatgata aaagcagtca taactagcat tttatctgcc aacgggtccg
    35941 caaacttgcc aaaattagtg acaaggcctc gcgcacgggc aatttttcca tctaaccagt
    36001 ctgtgatact agctaaagca aaaataactg cacctactag ctgtgtgaca gccagtgtgg
    36061 tgtctcctac gttaaattgt ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg
    36121 ggatcataca aattctaagt acagttaatt tatttggtaa attcacacta tcgactcctt
    36181 tcttccctat tctacgtgac tatcagcgaa gtttccaccg atattattta cttttccaaa
    36241 tgaatttatt caaaggagta tttttattga aattgatctt tttgaattaa cgaagaagga
    36301 ccgcgacaca gatttgtcac gatccttctt ttttatacct acaagtaaac agatgcgtaa
    36361 actaatttat acgatcattc tgcgtattga agagtaaggt tcacattttt tggactctga
    36421 ttgttttccc ctggattaac tggtacggat tctccattcg ctttgatcgt tgcattgctt
    36481 gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg gtaacacggt actctgtgtt
    36541 tcatttgctg ccagtgtccc ttgataaaca taggcattgt ctactagcac acctaaccat
    36601 acacgattga cagcagtaaa ttcaagggtt acaggcttgg tagcgtctgt gatatttact
    36661 gcgatggcac tgccagaatc ttctcctgta gctattgcca ttttcttttc ttcacttgat
    36721 tttgttgttg attctactgt agaagactct ttggtctgcg tgctttgctc tgaggaagag
    36781 gactcctcga ctgatccttc tactttaagc gaagaagctg tgtctccgat tattggatca
    36841 gcttgacgat cttgccaagt catatagccc acgacaacga caatcgccaa agcaaccaat
    36901 cctaataaaa tgacaggaag acttgtccaa aagcgacttg gatgcgattc ttcctgatgc
    36961 attgcttttc tagaagtact tactgtttca ggctgcggac gttcagggac cgatcctgtc
    37021 aatttgcttt tcccatcaaa aacatcgact aaatggtctc catcttctcc tacagcttca
    37081 gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag gcaattggtc aaaatcatca
    37141 ttgtcgattg cttccaaata acgtttttga atttttgtaa tctgctgtaa ttcatctaaa
    37201 gatatttttt tattcaggcg tgcttgccgt aatttttctc cgatggtttc aaaagccact
    37261 cgttcatctt ctccagtctg tttgattctc cgaagcatct gctgttgcaa cgaaagacgt
    37321 tatgtttggg tgctgtgaca aaagtcatgt cacagctcct tttttccgaa taaacattgt
    37381 tctcctgcca actcctcggt cacaagtcac aatatccaat aaatataaga agctgttttt
    37441 tgaacttgtt ccttgtgact cggagttaaa cggcaggctc acgacctctt tcttaaacgg
    37501 tgttcaatca gctgttcctc ggtattagct caaaaaccgg caaaatataa ggagatattt
    37561 ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac
    37621 ggtgttcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac attaagaagc
    37681 tgtttttact tggctgttct tattacttga tgcagggcag ctttttcaca acctcttggt
    37741 acacggtgtt ccagaagtag cttcttcggc aataagccga aatatcacaa aaatttgaga
    37801 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctctt
    37861 tgattacttc gtttgatttt attttaatat aagttgatta aaagagagca tgatttgttt
    37921 tattttctca ctaatttttc caacacaact aagaatacca taaaagctga ttttggagga
    37981 ggttcctact acaagtctaa aacgaaattt aagacttttc ctcataaaat actggtttat
    38041 tttggtctca tgtaaaactc gctgaaggcc tcttcactta taaatgcttc cccagctgcc
    38101 agtacgtctt tcatttggat cgaatcgatg atttcaggaa gatcaaataa agttcgatcg
    38161 ccaaataaac tttgcgtaaa ttgattggca atatattcga tcgaatttaa tgattggaaa
    38221 tattgtccaa gcattttctt cttcagcaaa tcgagatttg tttctgacac ttccggatca
    38281 tcagcgaatc caagaatgat ttctttgact ttttctgctg ctttctctgg ttcgtccgta
    38341 tctccgctga agtccgcaaa gtgaaattca cgatccaagc tgaattcaaa accaaagctg
    38401 tcatcgataa ttccttgatt gtacatcgct agatagtttc gagaagtatt gcctaaaatc
    38461 atttggaaca acagattgat tgctgtttta tagcgtaaca gttctgttcc ttcttgcggc
    38521 aatgtgtcta atcctttaat accaagtaca aatttatctc gtgtaatggc tgcttccaat
    38581 gcactttgtt tgatgatttc ttttgtgttt tcagggaaat aacgtacaat ttcttgcttt
    38641 ggagggaaat tttttgcttc ttggttttcc cgaataagtt tcatcaattt ctcaggctcc
    38701 atcttaccaa ctacaaataa aaccatattg cttggctgat aaaacgtccg atagcacgta
    38761 tacaagtctt gagctgtgat tttgtcgata ctttcaaccg tgcccgcaat atcgatatgc
    38821 aatgggtgtg taggatacaa gttatttagt atgccaaaaa acatccgcca gttagggtcg
    38881 tcttcataca tttggatttc ctgaccgatg attccttttt ccttgtttac agtttcttct
    38941 gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca gattcttttc tacctgatcc
    39001 gtcgtagaaa acagatagct ggttttcgta aaacttgtga aagcattggc ggaagctcct
    39061 tgtttcccaa ataattgaaa gacatcacca tcttcttttt caaacaactt atgttctaaa
    39121 aaatgggcaa ttccatctgg aactttcacc ttttcttttt caccataggg aataaattcg
    39181 ttatcgatcg aaccataatt cgtactgaac aaaccatatg tcttgtgata gtcattttta
    39241 ggcaacaaat acacggtcaa accgtttggc agtacttcat gataaagtgt ttcattgatt
    39301 tgctcatatt ctgttttatt catttgcatt tcctcctgct aaaaagaaga ttgcctgcaa
    39361 ttcgatttgc tcagctactt gttgtatctc tttgatcgtt acgctctcca tacgtttcaa
    39421 ccattcttca tcagataatt ttgtttccgg cagccagctg tccaaaaaag aagcttcgat
    39481 tgccgcttgc ggagagtcta gagaaagcag atattggttt ctcagcattg cttttgtctg
    39541 agacagttcc aactctgtta tttcaccatt tcgcaaactt tctaattgct cgtgaatcag
    39601 atgaagtact tgatttcggt tcttttcatc gataccggtt tggacactca tgaatcctcg
    39661 gaacgtatcc acgctactag aggcataata agctagactt tctttctctc tgacattcat
    39721 gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta aagaccatta gcgcaaaacg
    39781 ttctggttca tcgtaataca catttgtctg ataagctagg ttcagctttg cttgaacgat
    39841 tggctcacgt acttgtcgtt ccttcacgat attcacttga ggttgggtat aaaacagatc
    39901 tggataaact gtttcccgcg actcaaatgg taattgccca actgcttcag ctgctttgtc
    39961 cggatcaaca tcgccgacaa caaaaatgtc gatttgatca tttgccatca tttcttgata
    40021 gtaggacgct agcgaactag cagtgatctt gtccaaatct tcgactacgc ccaaccctgg
    40081 gatcttttgt tccgggctgt tttcaaaata aagttcttgc aaggcatagg aagcaaaagt
    40141 ttgtttatct tccttcaaac tttccaaata tagacgcaga ttgttctttt ccaagtcaaa
    40201 cgtcaactga tcaaactgat gatttttaat atttggataa aataaaacct cttttaaaaa
    40261 atctaccgct tgagaaaaaa ggtttggatc attgacatat tcaccattca caaaactgat
    40321 ccctgcgttt agccaatgta aatttccttt ttttctaact gataatccaa agcttgcgcc
    40381 ataaagctct gcaagttgcg cacttaactt tgtctgatcc gaataattca aactatttgt
    40441 ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact tcttgttgca gacgcgcagt
    40501 aaaacggata aaaatccgga tcgttttgta ttgttttgtt ggtaagacat gaagattcac
    40561 gccttctgcc aattgataca ccatatttat atgctccttt tcacagttga ttgcccgctt
    40621 actcttttaa tgggtcaacc tgcctttttt acttattcct ctgcttgaaa tttataagtt
    40681 caggcaattc gccgtttttt ctaaatgatg ttacacttta tagtatagca tagatgtttt
    40741 ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt tttatttttt atttttcgat
    40801 atactaaaga caaataaaag aagggagggt cattcatgat caaagaattc aaagagttca
    40861 tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt tattggtagt gcgtttacaa
    40921 gcatcgtaaa ccaagtggtg caaggcttga tcacaccact aataggttgg tttgtcgcaa
    40981 tgatcacagg tacttctgat ttagaaggag cactttcaat tcttgactgg tcgccgacaa
    41041 aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat cattacattt ctgatcactg
    41101 gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc gaagaattta cgctccaaag
    41161 aagagcaagt ggaagtggaa gaaactccta ctgcagaaga ttatcttagt gacatacgcg
    41221 atcttcttgc caaacaagtg gcagaagaca atacacatcc agtagagcaa acaaatcttt
    41281 ccgatacttc taaataataa agaatctaaa aaaaagaaag ccgattttca gtgtcggctt
    41341 tcttttttta gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc
    41401 atcttccaaa tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag
    41461 gtattcttgt gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat
    41521 cgtttcttga acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac
    41581 acgtccaaaa tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc
    41641 atcagtatct tgaaaaaacg tatgccaagc ttctccatca atgtcatcaa cttcttgata
    41701 aggacactgt tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc
    41761 tgctgttgac aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc
    41821 ttttttattc ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca
    41881 ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga tctcaactgt ccgagtaagt
    41941 acatctgaat agctgtaaga tactcgttca aaagaatttt cttcaggatc caaatcaaca
    42001 acgaatactg aaggataggt ttctgttaaa acgccgcggc gttctgtttg acgctttctt
    42061 cctgtctgag caaccaacat gattgggctg ccgatacgac cttctaaatc tttcttgatt
    42121 gaagctaaag ttgttggcat acagttcacc tctactatca actataccac atatttttaa
    42181 aattttcaag tttaccataa aaaaaaaaga aatgcaataa aaataaataa gaagctcatt
    42241 aagatttttc taaattattc ctcattataa aatttcaagt gtttttttat tttgagtttt
    42301 actctcccgt aagcgctcat tacttgcttg tgatccattg ctaaacattc agccatgtct
    42361 acaaaatcaa cgccttgcat ataataatga aaaattttag cttctatttt tgaaaactgg
    42421 atttcgcaat tttcgattgc ttcttttaat aaaagatgct gaattgctaa aggttcttgc
    42481 aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc gattgagact
    42541 tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga atagatttga
    42601 ttgcttataa cgcttttata ataacttcca aagctaacac ctttgctttt ttcgtaagtt
    42661 ctcaatgcct gatataaagc gatccttgct tcttgttccc aatcctcaga aacataattt
    42721 cttaaataat aggtgttttt caaacgtagt aaaagtggaa aatatttttg atacagggca
    42781 gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata actcctcaga cataaaataa
    42841 ttcctccata aattcgttta tggaggaatc ttatcatcaa tgattcatcg aactgattag
    42901 aacgatatgt tatttatttt ttctcattaa atcgtccaat ttttgggaca atttttttaa
    42961 ctgctcttca ttccatggag aattacgtcg gaaatcaata aaatgcatat ctttattttc
    43021 ttgggctatt gttttttccg ttttttttat cgtcttatat aattcataag cagaagttcg
    43081 taatgctcct tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg ccactgtcac
    43141 ttgtgtcaaa cggtcattca gctgaccagc gattctctcg atataactgt cagccgtttc
    43201 gtcttctttc gtaaaaacaa catccaactg gtatttctta taattttgtt ggatgcccgg
    43261 aactagttgg gcatcgaaga caacgatgac ttccattcct tcatatttcg cataatttga
    43321 taaacgatgt aaaagagctt ctcttgcatc cgccattttg tcctgctttt taagctggac
    43381 aagttccggc cacgaaccaa tcatgttgta gccgtctaca atcaacagct gttttttcat
    43441 ggtctactcc ccctgacgtt gggaaaaagc ctgatacata agcaaaccag cagcaacact
    43501 ggcatttaag ctctggacgt gcccggtcat cgggatcgtc agcaattcat ccatttcttt
    43561 tttcaatcct ggactcattc ctttgccttc attaccaatg actaaagcaa ttgatccttt
    43621 ggcattccat ttttggaagt ttgtgccggt catatctgtg ccaaataccc aaaatcctgt
    43681 ttctttgagc tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag ggacatgttc
    43741 gactgcccca gtggaagtct ttgttacgat aggtgtgatt ccaactgctc ggtgttttgg
    43801 tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc caaaattgtg
    43861 tggatcttcg atgctatcca aaatcaaaaa gaaggggtct tcttttttct cttttgttcg
    43921 ttcaatcagt tggtcaaggg ataaatattc ataaggtgta atcgcaagaa cgactccttg
    43981 atgcacgccg tgatcgctca aagtttctaa tttttgttta ggaacccatt tgactggcac
    44041 agcttgttca cgtgcgattt ctttcagttc ttctactttt tctcctcgtg aatcttcttg
    44101 aagaaagagt ttattccctc gcccttgttg tagcgcttct actgcggcat ggtgcccaaa
    44161 gacgaaatta tcttctaatg cttcttcatt ttgatcagaa actgactgat ttcgtccagt
    44221 ttgcttttta tttgaatatc tattttgttt tttgtcttca aatccacgtt tcgattgatg
    44281 tttgtttgtt ctttctttac ccattttttt caccaacttt ctgaatacac cagcggatca
    44341 attcttccat ccgctcggtt tgttttgtca aatgcagata gcccatgagc gcttcgaacc
    44401 ctgttgcgac ccgatacgtc gtaacatcag cattttttgc gctcgtatgg cttttagcat
    44461 ttcttcctct gcgataataa agttcttctt cttctgtcaa aagatgttct tccagcattt
    44521 gctgaatcaa aaaggcttgt gcattcgcag aaacataatt cgttgccgca cgatgcagct
    44581 tattgggttt tgtttctcct tgatcaatca ggtggtcacg aacatagatt tcgtaaatcg
    44641 catctccaac ataagccaag gctaaaccgt tcaattgtgt gtaatctctc atgcttctct
    44701 tctccatcgg gttccttgcg gtgtatcttc taataagatc cctttttctt tcaacaaatc
    44761 acgaatttcg tcgcttcgga caaaatcacg attgcttctt gcttgatttc gttcttcgat
    44821 caattgttca atctcttcat ctaataactc ttctgctgta aataaaatac cgaagatcga
    44881 aagccattgc gagaataaag cttctccttg tgccaagaca gcggcagaaa ctttcggctg
    44941 gtctagataa gtgttcagcc acttagcaag ctcatagacc actgtgatcc catttgctgc
    45001 attaaaatca tcgtccattt cttcgatgaa tcgctgttct aaattttcta acaaagcgat
    45061 tttatccgca tcatctgcca aattctctga ggcagtttct tggcggaatt tcaaattgtc
    45121 aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg tttcactgta
    45181 gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt gtggatcaac
    45241 gtttttgatc atttcatgta cagtgatgaa attccctaga gatttactca ttttttcatc
    45301 gtcttcgcca atcgttacat agccattgtg catccagtaa ttagcgaatt ttttccctgt
    45361 tttcgcttca ctttgtgcga tttcattttc atgatgagga aattctaaat cttgcccgcc
    45421 accatggata tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac attcaatatg
    45481 ccaaccggga cgtcctttgc cccatggtga atcccaagaa atctcgccag gttttgcttg
    45541 tttccataaa gcgaaatcta atgggtcttc tttttgctgc tgttcttccc ctgtacgctg
    45601 acttgctccg acttccagtt catcgatgga ttggtcactt aatttcccat aattttcaaa
    45661 tttacgggta cggtaataca catcacctga agattcataa gcatagcctt tttcaaccaa
    45721 cacttgaata aatgctaaaa tatctggcat atgatccatg actcttggat gcagtgtagc
    45781 aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac gatcagcaac
    45841 ttctggtgct gtaataccta attcgtttgc tgtacggatg attttatcgt ctacatccgt
    45901 gaaattcgat acgaaatcga ctttatagcc tcgatattca aaatagcgtc gaattgtatc
    45961 gaaagcaatg gtgctacgag catttccgat atggatataa ttatacacag tcggtccgca
    46021 gacatacata ccgacatgtc ccttttgtat agggatgaat tcttcttttt ttctggtcaa
    46081 tgtattgtag atttttatca ttctgatctc ctcacttttt ctcctttgat tcgaacgact
    46141 tttgctggta ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac aacggcacct
    46201 gcaccgattt tggcgttttt tccgatcgtt accggtccta atatctgcgc attcgcagag
    46261 agcatggctc cttgtttaac agttgggtgt cttttacccg tttcttttcc tgtgccgcct
    46321 aatgttacgc catggaataa aatcacatcg tcctcgattt ctgcggtttc gccaatgacg
    46381 attcccattc catggtcgat gaacacgcca gttccaatcg ttgctcctgg atggatctca
    46441 acaccagtta ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa gagccggtgc
    46501 tgatataaaa aatgagagaa acgatgccaa aacaaggcat gaaaaccagg gtatgtcaaa
    46561 acgatctcag ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc aatcgctcgg
    46621 gtaaaccaac tcatgacttt ccactccttt cgtaagaatt ttatgtagga aatcatcttc
    46681 ttataaagaa aagaagcgcc ttttaaataa gtttccttat caaaagacgc tttccgcgtg
    46741 gttccacttt tcttcataat caatgattat ctctgacagc taacgcctgt tatacgttct
    46801 tggctacttt gtttcctata aaacagtttt tcctgttttc tgtgtttcgc caagaccact
    46861 cccagaggca tttcggcaac cattcattga taccttgcac cacccggtat ctctcttgta
    46921 atgatcagct gcttactttt ttctgttcat cgtgtttagt tcagtacttg atttaaatgt
    46981 gctaatgatt tttcacgacc aagcaattcg atcgtttcag gcaattctgg accatgcatt
    47041 tgtcctgata cagctacacg gattggcatg aaaagatttt ttccttttac gccagtttct
    47101 ttttgaacag ctttgatggc agctttgaca gttggagcat ctactacttc catttgttct
    47161 agctgtgctt tgaatgcgtg gagaacagta ggaactgttt caccagcaag tacttctttt
    47221 gctgcttcat tcaatacagg atgttcattg aaaaatagcg tagacagatc aacgatttct
    47281 gcagcatagc tcatttgagg ttgatacagg ctaacgatct tcttcaccca ttcgattgtc
    47341 ttttcatctg ggttttctgc tacacgtcca tcagcgatta gataaggcag acacatgtca
    47401 gtaaatgcat ctagatccat tgctttgatg taatggttgt ttacccattg cagttttttc
    47461 gcatcgaaag ctgcgggtga tttacttagg cgttttggat cgaacatttt gatcaattct
    47521 tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc aataaagttg
    47581 aacattgcat ctggtagata gcctaattcg cgatattgtt cgataaattg caagattgat
    47641 tcgtcacgtt tgcttagttt tttacctgtt tcactgttga tgatcaatgt catatgtccg
    47701 aaggttggtg gtgtccagcc aaatgcttca tagatcatca gttgttttgg tgtattggca
    47761 atatggtcat ctccacgtaa aacgtgagtg attttcatca aatgatcatc gaccgcaaca
    47821 gcaaaattat aagttggcat accatcgcgt ttttggatga caaagtcgcc accgatatta
    47881 tcggattcga acgaaatagc gcctttcacc atatctgtaa atgcgtattc tgtattgcga
    47941 ggcacacgga aacgtacaac agattctaga ccttgtgctt ctttttctgc ttgttcttca
    48001 ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc gcgttgtgct
    48061 tcccgttctg cttctaattc ttcttctgtg caatagcatt tgtatgctcg attgctagca
    48121 agcagctgat caatcagcgg ttgatagatt tctttacgtt cagattgacg gtaagggccg
    48181 tattctccag gattttcagg agattcatcc caattcattc ccaaccaagc aaggttttcc
    48241 aactggcttt tttcgccatc ttcgatatta cgtttttggt cagtatcctc aatacgaata
    48301 ataaagtctc catcattgtg gcgggcaaac aaataattga ataatgcagt tcttgcattt
    48361 ccaatatgca ggtgcccagt tggacttggt gcatagcgta cgcgtacttt cgtcattttc
    48421 ttcctcttct ttcaaactag actttgacta acagcaaaaa ttccgtcagt tacggcttct
    48481 caaatatcat ttatttaaag ccactcagca caaattttac cttttctaag gtaaatagta
    48541 aagccaactg gttgaatttc cttaccagtt ggctgaattt cttttttatt ttgattgttt
    48601 agtctttttc tctgtctctt ttttattgtc gattcctcgt cctgcatgtg caggtttagc
    48661 aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt acaacgactt cgatacgttc
    48721 attcatgtaa tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa ggtaggctac
    48781 tccttgttgg cgttctgtcc cggcttttac tactagaaca ttcatcgttt caccagggat
    48841 gacgactggt tttacggcat ttgccaacgc attgatatta agaacaggta cattttggaa
    48901 ttcagagact ttattcaagt tatagtcatt ggttacaatg acaccatcta aaagcttagc
    48961 caatttgatc aatttgctgt ctacttcact gatatcttca aagtctccat cgtacatttc
    49021 gacagatata ccctcttctt tttgcaaggc atttaggata tctaatccgc gacgaccacg
    49081 cacacgtttc aaactatccc ctgagtccgc gatgtattgt agttcgtaaa gaacaaaatt
    49141 agggatcatc aaggttcctt ctaagaatcc agtctttgta atatcataaa tccgaccgtc
    49201 aatgatcacg cttgtatcca agattttata tttatggaaa ttttctcctg attttcgttc
    49261 taacagttga ctctcaactt gactagctgt ttcttgctcg atttttttgc ttcgtgaacc
    49321 aaaaactttc ttccattctt cgattcgtgt cgttcccatt ctcaatccca gatagcctaa
    49381 gatgatcatc agtaaaatcg gcaatacact attgacgaat ggaatttcca tattgtaaag
    49441 aggggtcgag atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg caccgaagag
    49501 aagataagtc aagctgacct cacttagttt ttgttctact tgtttgataa tagctgagat
    49561 atgtttggcc aacagcaaag ataataaaaa gaaaataagt gcgcctaaca ggctgttggt
    49621 aaatgtatta ttcagccatc cgtttgactg ttgaccgatt gcctgccaag ctgagggtaa
    49681 aaatgaaata cccaagcttg cgccgacaat gatcatcagc aaagtaatga cacgtttttg
    49741 catcgttttt cctccgtttc gacaatcatt tattagttag cggaacacac gcttcaacgt
    49801 ttcagccaaa gttgctactc caactatctc gattcctttt ggcgcttccc agttacctaa
    49861 gttgttttta ggtaggtata cttttgtgaa gccgagtttt tggacttctt tgactcgttg
    49921 ttcaatatga ctgacacgtc tgatttctcc agtcagtcca atttcaccga tgaaacattc
    49981 cgttggctgt gttccttttt ctttatagct tgaagcaata ctgacagcga tagctaaatc
    50041 aatcgctggt tcatttaatt ttaccccacc tgcagctttt aagtaagcat cttgattctg
    50101 aagaagcaac ccagctcttt tttccagcac tgccatgatc agagataccc gattgaaatc
    50161 aagacctgtt gttgttcgtt tggcatttcc gaacatagta ggtgtgacta atgcttggat
    50221 ctctaccaaa attggacgtg tcccttccat tgccacaacg atagcagagc cagttgcacc
    50281 gtctaggcgt tcttctaaga aaatctgtga aggattagcg acttcttcca atccatgctc
    50341 atgcatttca aaaatcccaa tctcattcgt tgagccaaat cggtttttta ctgctcgcaa
    50401 aatccgaaaa ctatgatgtt gttcaccttc aaaatataac accgtatcca ccatatgttc
    50461 taacattctt gggccggcga tcgagccttc tttggtaaca tgaccgacga taaaaatggc
    50521 tatcccattt gttttagcgg ttttgagtag ttctgcagtg gtttctctta cttggctgac
    50581 acttcctgcc acacttgtga tgtctggctg cgtcatcgtt tggatggaat caatgataac
    50641 ataatcaggg gagatatgtt cgatggcacg actgatttcg ttcatatctg tttcagcgta
    50701 tagataaaat tctgtgttga tacttccgag gcgttccgcg cgtaatttta tctgctctgc
    50761 gctttcttcg ccggatacat ataagacttt tccgcctatg gctgctaatt gctgagaaac
    50821 ttgtaacagc aaagtggatt tcccgatacc tggatcgcca ccaatcaaga ccatagaccc
    50881 tggcaccaca cctccgccta acacacggtt cagttcttct aattttgttt tgatccgagg
    50941 ttctttttta gggacgacgt ctgcgatttt tgttggtttt gctttttctc ctgtcaaact
    51001 tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg tattccattt
    51061 tccgcaattt gggcagcgtc ccaagaactt aggcgacaca tacccgcaat tttggcaaac
    51121 aaattgtact tttgattttt ttgccattgt accgcctcct tttatcctaa tcatgagaca
    51181 ttaaatcaag cttaagtatc tttaacgttt tatttactgg aagaaccaaa tccgccttga
    51241 cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat cccttggccg
    51301 atacgctcgc cttttttgat cactacatcc ttgaaaccaa agttggtgaa ttggaacatg
    51361 atatggcctt catttgcctc attattgtaa taatcactgt caatcacacc tactccgttt
    51421 gtcaacacta aaaaccgctt caacggattg cttgaacggt tagcaagctg caaaaattca
    51481 tcttctccca tgtaagcttt gattccagta ggcacgaggg ttggtttcaa ttgtttttca
    51541 ttttctgata catctttttt caatccatct gcgactgttt ccaacccctt tttccagcct
    51601 gctttccaga tacttggaat cacgacctct tctgctgctt caaaatcata gccagcagca
    51661 tgatgtgtag cgcgctgcgg tagattgata ttttgatctt tatatttaga aataacttca
    51721 aatcctcttt ttctcaccgg ttttcctccc taaataaact actcctattc taacatatcc
    51781 gaggctgtaa aagactttct ttacaaattc ttcaagaaat tctgctatct taaagtaaac
    51841 cattaattcg ttctttttcc aaacgactgc aagttgtaca aaaaaactat tttttacatg
    51901 actttactcg tcaaaacaag tagaatagaa cgaaattaat gagtggatat ttgattgagg
    51961 tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt aggaacaagt
    52021 acattgattt atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt agcttttgtc
    52081 ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg agctgtaggc
    52141 gttggtatgc ataaattaca tttgtcagaa cgtccgactt tgattcctga acagcaagct
    52201 gcggcggctg ttggtcaagc cgaactgatg aatatctata atcaaagatt cagtgtatat
    52261 aatcaacaga cggcacagat cctcgtgaca agagacgtgg tagattatcc tgaaagtcgt
    52321 aaaaatgtga ggaatacatt ggaacagctt ttgattatgg acacgatccc tatcgtgaac
    52381 gaaaacgata ctgtagcagt ggatgaatta gatcatctga caaaatttgg cgataatgat
    52441 cagctgtcag ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt cctttctgat
    52501 atcgacgggt tttattcaga taatccgact acaaatcccg aggctcagat gtatcgctat
    52561 atcgataaaa tcgatgaaaa gattctggca caagcaggtg gtgctggtag cacatttgga
    52621 acaggcggta tgcagagtaa attaaaagcc gcttctagga tatttgaagc aaacagcgca
    52681 atgattttag caaatggaaa aaatccggca attatttttg atattcttgc aggaaaagaa
    52741 gtaggtactt tatttaagga ggacttgaaa tgactgactt gatgcaatta ggacaacagg
    52801 caaaagatag cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat gagctacttg
    52861 ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat cattgaagca aatcataaag
    52921 acttagaaaa agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc ttgcagctga
    52981 cacctgagcg aatcaatgcc atggctacag agatcgaaaa aattgcttcg ttagacgatc
    53041 ctattggaaa agtagatgag atgtggacga acaccgacgg attgagaatc ggcaaaaagc
    53101 gcgtgccact aggcgtgatc ggcatcattt acgaatctcg tccaaatgtc acgacagatg
    53161 cagctagtct agcattcaaa tcagggaatg ccgttatcct tcgcggtggc aaagaagcat
    53221 tcttctcgaa tcaattatta gtccaattat tgcagcaagt actggtcagt gctggcgaat
    53281 ctccttatgc catccagttt gtcgatgata cttctcatga aactgcagga cagttgatgc
    53341 gattgacaga atacttagat gtgctgattc ctcgaggcgg agcaaaatta atccaacgag
    53401 tcaaagaaca ggcaacggta cctatcattg aaacaggtac aggaaataat cacgtatata
    53461 tcgataaaga agcacagtta tctatggcag tgaacatcat cgtcaatgca aaagcttccc
    53521 gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc gcaccttttt
    53581 tcttgccagc tattgaaaaa gaactggtag aacacggggt ctctttacgt gcggatgctc
    53641 gtgcgttaga atatctagag acggctgctt tagcagaaga cacagattgg gatactgaat
    53701 atctagatta tatccttgct gtcaaagtgg tcgattcttt ggatgaagca attgcacata
    53761 tcaatcggca taatactaaa cattcagaaa cgatcgtgac agatagttat gcggctagtc
    53821 aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc actcgtttta
    53881 cagacggatc ggtatttggt tttggtgcag agattggtat ctctactcaa aagttgcatg
    53941 cccgtggtcc aatggggtta aatgaattaa cttccacaaa atatatcatt tatggcgatg
    54001 gacaaatccg cgaataatgt ttcgtgtgaa acattattcc cgccttttgc cttttttctg
    54061 taaaggttct atccaactag aaaggtgtca atatgtttga aattttaatg atttttttta
    54121 tttaccttat ttctctgaat atcgctgctt ttctgggagt cggtatttta tcccttttct
    54181 ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa atggtcgcag tattttgaca
    54241 aaatcgggca aaaagggctg gttacacgct tgcacattag ttacatggtt gcattatgct
    54301 tattagcgac aatcaattac tattcttttt tcgatcattc aatcgcttat acgattacgc
    54361 tactgattgc tgggattttt cacttatctt ataaatatca gctgaataaa aaccacttga
    54421 atcggacttt ccgctaaaaa atatccgtac gataagtaga gtgccttgaa gatcggcaaa
    54481 aaactgccgg actttttaga ggctgtgaca aagattttgt cacagcctct ttttccatat
    54541 aaacggcgtt cgcctaccag ctgcctggta aatctgtttc ctcttcgtta agaagcgaaa
    54601 atccgtcaaa tgtttcgcca tcataataac gaagcagctc atccatggac aagttcatcg
    54661 ttttttcttt ttcttcttcc gctgattcag ccagtagctg ttcccgatac acctgataca
    54721 taccagctat tttttgctgc atcgcttttc tagtttcacc ttccgcattg attattggca
    54781 attctttgat atagccacga taaatcaacc gatcatctga aaattcaatg ggtaattcaa
    54841 cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat ctattatccc cccattcttt
    54901 ttcttaaatg aattttagct tacctactga aaattttcca gtttcattat tttgagaaaa
    54961 aaagggaatg tgacaaaaat cgtgtcacat cccctttttc cgaataaatg gtgttcttct
    55021 gccaactcct caatcataag tcacaatatt caacaaacat gagaaactgt tttttgaaat
    55081 tgttccttgt gattcagagt taaacgacag tctcacaacc tcttggtaaa cggcgttcaa
    55141 tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata tgagaagcta tttttccgtt
    55201 ttgtttctct aatacttagg ataaaacgct gattttacaa cctctgttaa acggtgttca
    55261 atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt
    55321 ttgtttctct aatactcagg acaaaacgct gattttacaa cctcctgata aacggtgttc
    55381 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagaa gctatttttg
    55441 tttggctgtt cttatgactt gatgcaggac agctttttca caacctctga tttcttattc
    55501 ttcttcattt gtagcaaaat aattacggtg ataaatgata caacatacac tgataagaac
    55561 aatggcacct gcaaaataaa acgcatagct ttcaccaata tgttcaccaa tcacacctgc
    55621 aataaatggt gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg acgaagcaac
    55681 gtttcgttgc agacctggta tttccatagc aatcgtcgtc aacagtgtat tgataattcc
    55741 ttggaaaaat cctgctaaca caatgctgat cggtacaagg actggcatag aagttccagc
    55801 tcctaataac agcaaacaaa taatgaataa gacaaatgct gctaaaattg tccgataagt
    55861 cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca gaagcacacc
    55921 ccagccaaaa aatacaaaac cgacttctaa ttcagaatag ccaatcaata aaaatggcgc
    55981 atacgcaagt aaagtaaaga atccgaaatt atataacaat gcgattagtc caactgaacg
    56041 aagtttatga ttgcttaacg ctttgacacc ttcccaaagg cctacttttc tctttggttt
    56101 ctcagtttca ggtaaaagta ccgctagggc tataaaaccg caaaacatca aaaatgctac
    56161 cccaaaaaat ggataacgcc atgaaccact gcccaagacc ccaccgacta gagggccaac
    56221 agccattccg cagccaagtg ctgcttcata catcatgatt gctttttctg tgttaccaac
    56281 taaaatactg acgatcgttg ctaaagcagt agagacaaat aaagcattcc cgacacccca
    56341 gccggcacgt aacccaatca gcattccgat attcccagat aatccgccaa gaccagcgaa
    56401 tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc aaaccgatcc gactagataa
    56461 gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa ataacagggt
    56521 cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg ggtctactag
    56581 acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg aacttttccc
    56641 ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata tttccatccc
    56701 tttctctcta ttagcatcat acatgattta ttttacatgt caatatgcac atgtaaaacg
    56761 tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt tttatgaata
    56821 ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg tatgaattga
    56881 agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca acgcttgcca
    56941 agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct caaaaaaaga
    57001 tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa gaggaaactc
    57061 ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg acgctcgata
    57121 aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa atcatgcaaa
    57181 aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt ggacgttatg
    57241 ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt cagcaagtaa
    57301 gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta gaaatatcta
    57361 ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa aagcgatccg
    57421 aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca ttcaagagac
    57481 tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca ttcattactt
    57541 tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg caacggtaaa
    57601 gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg atcaggtatt
    57661 ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa aaccatcaag
    57721 catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag tttaaaggca
    57781 atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac cacgaatcca
    57841 ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca taaattatac
    57901 gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat tccttctttt
    57961 ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat cgtagcacat
    58021 ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat aaacttcatc
    58081 agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc ccggatatat
    58141 ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag tcggttcatc
    58201 taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc
    58261 tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt
    58321 cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat
    58381 ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc ttactttcgg
    58441 agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg tcttacctga
    58501 accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat caccatttac
    58561 cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg gatagcggct
    58621 gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct gcggcttggt
    58681 tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt ggatggcttt
    58741 ttgtacactg tctttttgtt tagaggaaga caaccgatcc agtctgattt gttgcttttt
    58801 ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt ggatcgattt
    58861 ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag tctttttctc
    58921 caattccttt tgagtgagat aggcactata attccccgaa aactctcgga tttctccgtc
    58981 ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat gactaacaaa
    59041 aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac tttttctatc
    59101 tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa gaagacgtat
    59161 caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt cttccagtct
    59221 taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga gataggttaa
    59281 ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg gaaaaagttc
    59341 accggctatt agtttgagca aagtcgattt accgacacca ttttctccta tgatccctat
    59401 acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt tgccaaagct
    59461 gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac tcctatttca
    59521 tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg
    59581 catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa atctgtcata
    59641 gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat gaaattgttg
    59701 tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac gtaatttcat
    59761 cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga ttcaaaaaaa
    59821 gcaatagcta ctgaatagta actgaaaacc ttttattcga acatagtgat cttttctggt
    59881 gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa aataataact
    59941 ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc ttcgaatcca
    60001 ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg catagccgct
    60061 aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact cttgtaataa
    60121 ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc tgattcgtaa
    60181 gcttggttgt agatttcttc gccatattcg taacattgca tatcgccaaa aatcatgacc
    60241 attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg caagatttct
    60301 tttgcttcct cgctttcagc gaccacaaag cgccatggtt tcatattgac agaagaaggt
    60361 gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca
    60421 tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc
    60481 attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc
    60541 taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc
    60601 tgtatcttta tggtccattt cacaatgctt gattttcttc tgggcttttt ctgttagcgc
    60661 tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc
    60721 caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact
    60781 gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact
    60841 tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata
    60901 taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc
    60961 aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta
    61021 ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg
    61081 gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat
    61141 agaagatagc tcttctgcag tagataattt tacaccgcca aaaatttccg aaatcgcctt
    61201 ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa
    61261 gttatgacaa gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa acttgcatct
    61321 gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg gctgttctta
    61381 ctacttgatg cagggcagct ttttcactac ctctttttaa taaccataaa atcctattcc
    61441 ttcatatctc cagcccatcg gtgttagcca atctctttcc ttagcactca aggtatagtg
    61501 atgggtacca gttttttcat ttggattaaa tagtcgataa attggcttgg aaccaccaga
    61561 ataaaaactt agaccttcat aacgccaacc gatttttgtt aaagagttga tctcagattg
    61621 tgccattgtg taatgatggt ctccactgta aggattgtat aaacggtaga caggttgtcc
    61681 gctgtttggt gcttgccaag caatcccttc atattcccag cctacagttg gcagataatt
    61741 tttttctccg gcattcaatg tatataaatg ttctcctgta tgacggttat acagacgata
    61801 catcggagaa gttgaagttg gtgctgtatc atactgtgtc agattatagg tttcgattac
    61861 attgttcaat gtagaaccat aattaggcgc agtggcataa cgacctgtta gccaagccgt
    61921 agcatctttg taagagttcg tattgctttt ccatgcaccg gaatagtagt aaacgcctgg
    61981 ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc aatgattctt tgtacgaagg
    62041 atattttcta aaagcggcat tcaccgtaat ccattgccca ttcacatatt ccgaagtagg
    62101 catcgtaaca gattggccat tatagcttcc tttaatccca aatagattgt aattaggggc
    62161 agaagccaaa gtactgttcc cccagccact ttcaacgatg gcttgagcaa tcatgactga
    62221 tgcatataag tcattggcac ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa
    62281 cgcttgttga gaattagcag atgagcgagc ataaaaagca tagccagtat agttttcgaa
    62341 ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc ccatctgtca attggttcgt
    62401 gatttcttct gcattctctg gattgttctg agcttctgct gatgtactgt cttcggtttg
    62461 tgtttgttct gttgtttcag ttgttggttc ttcggttgaa attgtatttt ctgagacagt
    62521 agctgactgc aaagtacttt ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt
    62581 ctgagttgcg acagtttctg tcgattcttc agctagcact gttgtgttgg caactgctag
    62641 atttcctaaa agaataacgc taaataataa attctttgct ttcatctttt ttctcctttg
    62701 aattatgaaa cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc
    62761 atatttaaat aacatttgtt tctttttaat aaatttgctt tttctgcata aaaagacgta
    62821 aatgaagaat attcttctcc attcacgcct taaattgatg gtcgtttgaa taactattta
    62881 acaagtaatt ctctctggat attatccgtt atggattata cttcctgtgt cgtcggacgg
    62941 ataatcatgt tgctgacaga catccggtct ggcgtttcta tcgcaaatgc tacagcagaa
    63001 gctatatctt cagatgttaa tccccattct ttttgcgctt catgaagttc aagagcagcc
    63061 tttttgtcgc tgatggtttg atataattca gtttgaacag ctcctggaga gatgatcgta
    63121 gatttgatgt tattttgccg ttgctcttga cgtaatcctt ccatgattgc acggacagca
    63181 aattttgttc cgcaataaac ggcggaatca ggataaacaa catgtcccgc cacagaatca
    63241 gttgaaatga tatgcccaga tttttgtttt tccatgattg gaagtacggc ggaaattcca
    63301 tttaacacac ccatgatgtt gatatcaagc atattttgcc attcatcacg gtgaccttca
    63361 actaatggag cggttggcat gattcctgca ttattgtaca atacatcgat tcgaccgaac
    63421 ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa aatctcttac atctgctgtt
    63481 tgaatatata gctcagcttc tggtaaagat tcttttattg cttttaggcg atcttctcgg
    63541 cgtgcagcta taactagttt agcccctttt tctgctaata gcttcgttgt tgcttcaccg
    63601 attccgcttg aagcgcccat gatgacaatc actttttctg ataaatcatt cattctattc
    63661 tcctcctgtt ttgaaaaatc tcttgcttat actcactatc tcttatttca tcttactgtt
    63721 caaataatcc gttcgacagg tttgacgatt ttggcaggga ttcctgcaac gatggtattg
    63781 tctgggacat ctttcgtaac agttgcattc gctgcaacaa tggcattctc gccaatcgtt
    63841 acgcctggca gaatagttgc tcctgctccg atccaagcat ttttcttgat gtggattggc
    63901 tttactgaca agcctcttcg ttcttttgga tcggttagat ggttcaccgt gatgaggttg
    63961 acacgaggac caatcaatac tgaatcttct aaacagatgc cacctaagtc aacgaacatc
    64021 acttgacgat tgataaagac atttttccct atactaatat gtttgccgaa atctgtataa
    64081 aaaggcagca gaacagtcac cgtatcatct actttgtcac taatgatatt ttctagccgt
    64141 ttgcggatat ctgctggttc atggtaccca gtattcagcg ctgctaaagg ctcttcgttt
    64201 tccgcttgga tctgatggat cacttcaaag agcggatccg ttactcgaat ctctttattg
    64261 acgatttgtt tttcgatgag gcttgtcatt ttgctccctc cttttatcct tactttataa
    64321 cggacggctt atgatgtcta atacttataa aacatactta actattcctt ttaactatac
    64381 ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg atattcgtat actgacgtat
    64441 tttttagcag tagctagaga aaaaaatatc agccgagcag caaaatctct gcatatcacg
    64501 caacctactc tctcaaaaca actgaaagat ttagaagaag aacttggtgt gacacttttc
    64561 actcgaggct cccgagagat acagttgacg gattctggtc gatatcttta ttcaaaagga
    64621 aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc
    64681 tctggagaaa tctacattgg cggaggcgaa actaaagcaa tctctttcat ttctgatagc
    64741 cttcattcgt tgatcaaggc gcatcctgat atccagtttc atttatatag cgggaatgca
    64801 gatgatatca tagacaaact ggatagcgga ttacttgatt ttggactcgt aatcgaaccg
    64861 acaaacaaac aaaaatacca gtcatttcga ctgccagccc aagatcgttg gggacttttg
    64921 atacatcgga atcatccttt agcaaagcaa acagtcatcc atccgcagga actgaaaact
    64981 gttcctttga tcatttcccg gcaaacgtca gtggacagtc aactagctga atggctaggt
    65041 ttcagcatag atcagttatc gatcgtcgga acatataact tattgtacaa tgcttctttg
    65101 atggtcgata acgggacttt tggtgcatta gctctagatg ggatcatcaa tacttatggg
    65161 acggaaaaag tattcatccc actcgatccg ccattacaag cttcactgaa tctcatttgg
    65221 aaaaaagacc agcctctttc tcctgctgct cgtaaatttt tagatgtcct aacggagaaa
    65281 tacagttaag tagagttttt ttccttcgct ttcagctttt taacaacgaa taagttaagc
    65341 aaaagatcca atgattaaat aacataagtt tcttaatttg ttccttcctt ttatttctga
    65401 taattccagt aaattactat aattataaaa aaagaattga caaaacataa ctgtctatat
    65461 aaaattgatt acgttaacaa taattcgttt catgtgacta gatcatacta ggcatgaaaa
    65521 atctctgagg aacatggttt ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa
    65581 tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca
    65641 aaaagatctt taatcaaaat gcagtattgg tagatgacgg tggacaggaa aaagtagcta
    65701 ttggaaaagg gattggattt gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg
    65761 aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga
    65821 ttgatgaacg ctttttattt gcagcagaaa cgatcattga tcatgcggaa actgttttga
    65881 tggaaaagtt gaatgaacat gtattgatcg ctttgaccga ccacattgct ttttctgcgg
    65941 agaacattaa taacggtatc gtcatccgca acaaactttt aagagaaatc gaagtgcttt
    66001 acagtgatga attcagtatt gctcaatggg cagtagagta tctgacaaaa gagcttggga
    66061 ttccatatac ttatgacgaa gcaggatata tcgcaatcca tattcacagc ggacgaagcg
    66121 gtcggaacaa taaccatcgc agtatccgtg aagtgacgat tgtatctgac atcatccatt
    66181 tagtggaatc agaattggaa acagatatcc attcagaacc attttcactg aattattcaa
    66241 gactggtgaa tcatttacga ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt
    66301 tagacacgga aatcgtagaa atggtcaaac gaaaataccc agaaagctat aagatttcta
    66361 aaaaaatccg tgtgttgctg accaaagaat accagctcgc gatcacgaaa gaagaactgg
    66421 gttacttggc catccatatc gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca
    66481 atcatgagga ggaaaaaaat tgaaagcata tctacaacga atgggacgtt cccttcaact
    66541 tccagttgcg gtcttacctg ctgctgcttt actagtaggg atcggaaact ggtgggcatc
    66601 ttatagtaac gacattgtcg ctcatttttt acaagctggc ggtaacgcag ttttaggtca
    66661 attacctttg ctgtttgccg ttgggttagc actagggatg tcagtggaca aatcaggtgc
    66721 tgctgccttg gcaggtttgg tcgcatttga agttccagta aatgttttaa aaactgattc
    66781 agtagctact ttattgaata tcaaaccaga atcagttgat ccatcttttg ctcagatcag
    66841 taacgtattt attggtatta ttgccggatt gattgcggca gctctttata atcgtttcca
    66901 cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc aagcgtctag tgccaatctt
    66961 ggctgccttt gtcatgttgc tgatcagtgc agtgctactg ctcgtatggc ctccggttta
    67021 ctctgcttta gtttcttttg ctaaattcat tttgagttta ggctggggtg gtgcaggtct
    67081 ttatggcttc ttcaaccgtt tactgatccc tacaggattg catcaagcaa tgaactccgt
    67141 gttctggttt gacgtagcag gaatcaatga tattggtaaa tttttggctt ctgaaggaac
    67201 aaaaggcgta acaggaatgt atcaagctgg atttttccca atcatgatgt tcgggcttcc
    67261 agcaggagca tatgctattt acagaaatgc acgtccagaa aacaaagcaa aaacagcttc
    67321 cttgatgtta gcagcagctt ttgcttcatt ctttactggt gtaacagaac cacttgaatt
    67381 ctcatttatg ttcgttgctt ggccattata tgtcttgcat gcaattttca ctgggatttc
    67441 tttagcagtg agtgcattct tccattggac agcaggattt gcctttagtg ccggatttgt
    67501 cgattacttc ctatctttga aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca
    67561 aggattagta acagcagtga tttactactt tggctttaac tttgctatca aaaaattcca
    67621 tctgatgact cctggccgcg aggaagcaga tctttcagat gacgatactg caacgactaa
    67681 ttcagacaat aaatatgcgg cacaagcaag caaaatctac gctgcattag gcggagccga
    67741 caatgtaact tcgatcgata actgtactac acgccttcgt ttgcaagtca aagatacagg
    67801 cctgatcgat caaaataaaa tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg
    67861 aaaaaatgcg caagtcatcg taggtacaga agtacaattc gttgcagatg agatggcaaa
    67921 actacatggt ggtgctgcaa cacgacctgc acaaacaaac accgtggtta aaacagaaac
    67981 atttgccggt ggaacgatcg aacaagatat ttacgctatc gcaaatggga aactgattcc
    68041 aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt
    68101 attaccagaa aatggagaaa tcttctctcc aatcgctggt acgatcacaa acattttccc
    68161 aacgaaacat gctgtcggaa tccaaacaga tgctggtatc gaagtcttgc ttcatatggg
    68221 catcaacact gttgacttaa aaggcgaacc attcacttta tatgtaaaag aaggtcaaaa
    68281 agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa
    68341 aaacacggat atggtcgttg tattcacaaa tggcgacaaa gtccaaagct tagaaatcga
    68401 acccgcacgt gatgtaaaag caaatgataa aatcggctct gtttccaaca aagcctgagc
    68461 catcgtcttc cccagacttt tttatcgata aacaaacagg agctgagaca aaaatcttgt
    68521 ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc aactccgcgg taataagtcc
    68581 agccaaacgt aacatatgaa gagctatttt ttatttgact cttcttatta cttgatgcag
    68641 aacagctttt ttacaacctc tgataaacgg tgttcaaaaa ccagtttatc ggtatgaagt
    68701 caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttcat actcaaaact
    68761 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    68821 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    68881 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    68941 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    69001 tttttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    69061 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    69121 tattcacgtt tcaataccca acctgttttt ccatttcctg cagcataaac gaattgacca
    69181 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    69241 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    69301 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    69361 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    69421 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    69481 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    69541 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    69601 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    69661 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    69721 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    69781 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    69841 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    69901 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    69961 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    70021 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    70081 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    70141 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    70201 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    70261 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    70321 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    70381 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    70441 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    70501 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    70561 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    70621 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgattaca
    70681 acaagatacg ttccttttgg gaatgggatg attcgggtag tgtttggaac atcctgactc
    70741 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    70801 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    70861 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    70921 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    70981 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    71041 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    71101 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    71161 gcttcgacta actcataatc tgaagaagga agccattctg catagatttt cccccaagta
    71221 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    71281 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    71341 tgcatcaaac tgcccttttc ttccaagcgg gctccatcaa atccataaga aacgttgatt
    71401 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    71461 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    71521 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    71581 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    71641 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    71701 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctaggag atcgcgatta
    71761 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    71821 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    71881 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    71941 tttatcttcc tccttgcttg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    72001 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    72061 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    72121 aataaatttt ggggaagaca cattctaagt aaacaaagcg gtaaaaatat cttttattcc
    72181 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    72241 gaacgctgct tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt
    72301 atttatttct aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc
    72361 atctttaaaa cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga
    72421 atgaattttt gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt
    72481 tcttaaacgt ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga
    72541 ataattggct tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc
    72601 acggttttgt ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca
    72661 cggaagatta taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc
    72721 tatatgccat agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag
    72781 aacgtccaat aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg
    72841 ctagttcctc tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcaattta
    72901 tcgaacgctt caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa
    72961 ctatcatcca taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt
    73021 tcatcatgga gatgcgaaag ttcatccaag aagcagttga atcgctcatt cagcaaaatg
    73081 cctatcttcc cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt
    73141 gggagcctga ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag
    73201 aattattagc agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc
    73261 aagtcgatca tactgacgcc tcttattcaa aacgctactc cataaaaatc ctgaaagaag
    73321 actttcggga aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa actaaagaaa
    73381 cggtaaagaa agacgatttc aatgaaaaat agcaagtaaa cgataacaac catcctaaat
    73441 ccacaagaaa tacagcagac cttgctttaa aaacacgaat catgcaaagg atgaagtaaa
    73501 gaagacttaa cgcgtaccca atattagtca tttgactatt aggtacgcgt tttgttttat
    73561 aagtcatgac aaatttcctt cgttgctggg tagattttcc ggcattgttg atagatttta
    73621 tgatactttt ctacattttg taagagaggt tccactgcat ctgtatattg gataaaattc
    73681 tgacaacaac tttcaggtgt gtcgaaccac tctaatcttt aaagataaat attaaaataa
    73741 cgattaataa tattaacagt ctttattttt caccaccaaa aacctatctt tgcatatact
    73801 aaatagacgt atatacacag ataggtttgt cttgtgaaaa ataatgatta tccttaccaa
    73861 aaaatgtttg tttttctaca tgttcgtact tatcaaaaaa tatgatcctt attgaaatta
    73921 ctggctattt atattccaac gacttcttgt gttaattttt gttatctcat agtaaattat
    73981 gtttcatttt cacttaattt agttgtcacg tgttcgtcaa ttcgtaaaaa cggtaaatat
    74041 ataaacacac ttgcggctat aattaatatt tgtaaaacag ctgctcgcca gtctccggct
    74101 gttgctaaaa atcccgatat aactggtggc gtagtccaag gaatttgcac tactacatga
    74161 ttaattaatc ccacagcagt tgcaaaatat gctactgtca aagaaaaaat cggtgcaagc
    74221 acaaagggaa taatcagact gatattaaat acaataggca atccaaaaat aataggttcg
    74281 cttatattga atatagcggg agttaccgat aatttagcaa agtccttata atcttttcta
    74341 cgactgaaga taaatattgc aatcaataaa gcaatcgtat ttcccgatcc acccattacc
    74401 gcaaaggtat ctttaaaggc catattgata atatgtggaa tttgctcttt gtttgcataa
    74461 gctgccatat tttcttgcat attctgaatc aaaaacggat ctaacaatga acctgatatt
    74521 actgcttgat ggataccaaa tccaaaaaat agattagcaa tagatgtaat taacaaaaat
    74581 cctggtaaac cagttgtaat tttacttaat ggctctgtaa ctatcgtggt aattagctta
    74641 tttatatcca tattaaacaa ttgaatgatt ataaatgaca ataaagcaaa cgaggtaatc
    74701 gtaataatga ttgggattaa tacattaaaa gactttaaaa cagcgggagg aatatttccg
    74761 gatagctgaa tctgtagttt cttattatta gaaagtttaa taaataattc tgtagccgct
    74821 aatccaacaa atatacctac aaacattcct gaagctcctg tataggatat aggtatcaca
    74881 cccgtcactt caacagcttt atccgcattt tcaggagtaa acatcatctg cataggtgtt
    74941 aatacaacaa atgtcgaaat agcaactaat accgctgcaa tatgatttgt ataatttctg
    75001 cttgcacaca tatgataaga tactgctgct acaactaata atgtagttat gcttaatgta
    75061 ccattggcaa ttgaagtacc aatttgttgt aacgtggtca atgtactagt atcgacaatt
    75121 tcccccatga atcctgttgg ttctaagatc actgaattaa taagtgtcac aaatcctgct
    75181 aacatcataa atggcataaa tgtagcaaac gcatctcgca aagttcttaa atggatctga
    75241 ttccccaact tagtcgcaac tataccaaat ttttcaaata caatgtcctt cctgctactt
    75301 ttgcccatat tttttctcct ttatgcttat tgtaatcgat tactttttcc acccaaataa
    75361 tacatcactt atcaaaaaaa gtaaatactt ttaattaaat atatatatct tttttagtat
    75421 aaaaaaataa gctctgaaaa aataattttc ccgagcttat ttttcaacaa aaacagcttc
    75481 aaaaacaaag tcttgatata aatgtctcga taccgaatac tcgaaaggtt taccagacga
    75541 tagagcaaca actttgtcta tctcaatcat aaattcacct ggatctattt ttaatatttt
    75601 tgcgtcaaat tcattcgcag tatctccttt gatacgtatg tgcgaagtct ttatttccag
    75661 acccagttca tttgtgatat aagcatatac agatttcttt aaatgttttg gttccaagcc
    75721 tggtatgact gctaatggca tgaatgtttg ctcaatcgag tatggttgac aatcaataaa
    75781 acggcttcgt acaatattat atacatagtc ttttacactg atacttaatt tatcagctac
    75841 ttttttcgat ggaagttcaa ttgagaaagt atggatttct gaatctacgt gttcttcacc
    75901 aaccacgcta aacaaacctt cgtttggacc aagaactcgt gagttatcaa ttggtgttct
    75961 gacgacttca gtcccatacc cacttctact tcgcaaaact ccttcggaaa caagtaagtc
    76021 tacgcctttc tttactgtta acttgcttgt atgataaata actgccaatt catctcctgt
    76081 aggaagtttc tcaccaattt tatactctcc atttacaatt tttttcttta tatctaagta
    76141 aatttccata tacttcgcaa tttttttcaa tggtgaccct ccaatcaaaa acatatcttc
    76201 atctatactt taacataaat aaaccgataa aatccacatc aaaattatac acacaataaa
    76261 aaagatatat atatttaaac aaaactattt actttaagtt gtaagcggtg ttatcattaa
    76321 gtggaaacag gaggtaaaaa aattatgaca actatgaaaa agctaacttt tccagatgat
    76381 ttttggtggg gttccgcttg gtcagctgag caagcagaag gaaaaggaga aacaggaaaa
    76441 gcagagacta tctgggatcg ctggtttaaa gaacaacctt accggtttta tcaacgaatc
    76501 gattcctcta ttacaaccga tcacatccat caatataaag aagatataga actaatgaaa
    76561 aaaactgggc ataactcctt tcgcgtttct atttcttggg cacgaatgtt tccaaatggc
    76621 ggtaccggaa aagtaaatcc caaagccatt tctttctatc gaaaattatt ttccgaaatg
    76681 aataataacg gaatcaaagt atttgccaac ttatatcatt ttgacatgcc tgctactctt
    76741 caagataaag gaggatggga atcacgtgaa gtagttgatg cttatgttca ttacgctgaa
    76801 acttgtttta aagaatttgg tgacttagtt tgtcattggt ttaccttcaa tgaaccactt
    76861 gggcctattt taggtactta tcttgaagat tttcattatc caaacctcat tgactttaaa
    76921 cgcggagcgc aagcagcatt cttcactata cttgcacacg caaaagcaat acaagtattt
    76981 aaagaactag ctcttcctag taaaataggc gttattctaa atttgagtcc tacttttcct
    77041 cgtagtcaaa atcctgctga tgtgaaagca gcagaaatcg ctgatttatt ttatacgcgg
    77101 agctttttag atcctatggt taaagggaat ttttctaaaa agcttatcct gttattacaa
    77161 gaatataatc agcttcctga agattatacg aaagaagatc ttgtatgtat ctacgaaaat
    77221 accgctcaaa ttcttggact aaactattac gagccacgtc gcgtcaaatc tcgttcttct
    77281 atggtggata catctagtcc atttttacca gagtggtttt tcgaaccaca tatcatgcct
    77341 ggacgacgaa tgaacgaata ccggggatgg gaaatttacg aaaaaggaat ttatgatttg
    77401 tgcatggata tcaagaacca ttatggaaac atcgaagcat ttatctcgga gaacggaatg
    77461 ggcgttgcgg atgaagaacg tttcatggat gatacagggc aaataattga tgattatcgg
    77521 attgagtata tcaaagatca tcttgcttac ttatggaagg caataaatga aggatgtaac
    77581 attaaggggt atcatctttg gacatttatt gattgctggt catggatcaa cgcctataaa
    77641 aaccgatatg ggctggtttc actcgacctt actactcaaa aaagaacaat taaaaaaagt
    77701 ggcgaatttt acaagcaatt aagtgataca aatggattta gatacgatgt agacttgtta
    77761 gttgaataat attctaaaat tccacttgaa gtgaacacat tagaaaacaa gaataatgag
    77821 aaataccaat aacaaatact ttttcttcat acttcattta tacttgtcag tataagagac
    77881 tgtgacaaat gtagtgatcc tcagaagtta ggtctggaaa tctaacttga gaagattttt
    77941 ttaaactacg tccagtcttt tttatttgtt taaaattgaa gttgacaagt cttcattaaa
    78001 aatttagaat attcacaagt ttgacgacaa ttatgaaaat ttatgtcgtg ctaaaggagt
    78061 tttgttattg gatgaaaagt tagatataaa aactgcgatc aaagacacgt tacctaccgt
    78121 ttttggttat atcggtattg gacttgcatt tggtatcgtt gggaaagctg ccgggtttca
    78181 tccgctggtc gtcacgttga tgtccctgct gatctatgct ggttctgccc aatttatcac
    78241 ggtcagcatg cttgctagcc atagtccact gctctccatc gttctctcga cctttctagt
    78301 caattcccga atgattctga tgagtatgac gattgctcct tatttcaaga aaaatcggct
    78361 gcttcaaaat ctgctgattg gcacattgct gacagatgaa agtttcgcac tgggaatgaa
    78421 taaattgaac tatactggac aaaagttaaa ctttcgttgg atgaatacag ctaattggat
    78481 ttcttatttg acgtgggtgg gttcttctct tgttggcgca cttttaggga attttattac
    78541 tgatccgaaa aagtttggtt tggatttcgc catcgtcgct atgtttatcg ggttacttta
    78601 tcttcaagtc atttctgata gaaacacctc taaacgatta cagttgatcc tgattggtct
    78661 cactttgatt ctggtatatg tcggactgat ttttatccca agtaacttag tgattttagt
    78721 tgtaacactg attgggtgcg gattgggggt gtggatcaaa catgccttct tttgagttta
    78781 cattgctgac tattttaggt tgtactttag caacttggct ttctaggatc ctaccatttg
    78841 tcttactgaa aaaattcgat ttgccgcaac cgttgcttga gtatttaagt tttgtcccga
    78901 tcgtcattat gtctgcttta tggttcagca gtctatttac ccaaaatatc ggacatctgc
    78961 cgcagatcaa tctcgaaaat gctttggctt cagttcctac acttttagct gctattcttt
    79021 caaaaagctt attggtgatc gtcttggtag gaattctttc cctttcgttg attcgcttat
    79081 tataaataaa aaaacaagag cttatggcaa attactgtca tgcgctcttg tttttatcaa
    79141 agtcaaataa gaaaaaaatc ggcgtattct tgcttatccg ttgaacggac cactaacaac
    79201 gacttttcct aatatatgtc ctgtttctac gtctttggtt gcttgtttca agttcttcgc
    79261 attcatccca tctgaataga ccttgctcaa tgtactgcgg ataatccctt catttgctaa
    79321 agcagcaatg tgcgctaaag cttctccttg cgtttcgatt tcatagttat agtctgtttt
    79381 agcgaacata tactcccagt cgaagctgat agattggttt ttccaaacac ctatatctaa
    79441 cggctctttg atccctacga tcgttcccac atgtccaaat ggtcggacaa ggtgtttgat
    79501 ttgatcaaag tagcgggtaa tatcgaataa tacagcgaca tactctactt gatcgattcc
    79561 taattcacga aaacttttac ttaagtcgtg atgataatcg attggatagt ctacacctgt
    79621 ttttttcagc cattcaaaat tcttcggact tgcagtcgcg tagacttcta ggccagccca
    79681 atgcgctagt tgattcaaaa tcgaaccgac gcccccagct ccgttgatca ctaagatttt
    79741 tttcccgcgg ttggcatttt cttctggagt cagaccaaat ttttcaaata acaattcata
    79801 agcggtcaat gaggtcaacg gcaaggctgc ggcttcttca tctgaaagat tttttggtgc
    79861 aagagcgaca atacgttcgt caaccagttg gtattcttga ttgcttcctg ctcttgtcgt
    79921 tgtacctgcg taaaaaacgc gatcgccgac agaaaatttt tgcacttcat cacctacagc
    79981 aacaactttc ccgactccat caaaccctaa gatacgtaaa gcatttctta tcccgttatc
    80041 ttggcgaagt ttcgtatcga caggattaac agatactgcg ttgactttta ccagaagatc
    80101 ccgtgaactg ggtacaggta tctgttcttt ttcatcaata aagctttttg gatcatcgat
    80161 cgacaaccct tcataaaaac cgactacctt cattaattcc ttttctgaca tacgaattcc
    80221 tccttagctt catcaagttg gagcatccat acagttttca tttcaatata tatagtatgc
    80281 tatttgttcc acttttattc atcataatta ttctcttact taaagtaaac tatttgcact
    80341 tttttggtta aaaaagaggt tgcgacaaga cttttgtcac aacctctttt aaacggtgtt
    80401 caatcaactg accctcggta ttcgctcaaa aaacgacaaa atatgaggag ctatttttgt
    80461 ttggctgttc ttattacttg atgcaggaca gctttttcac gacctctaat tcacggtgtt
    80521 caagcaagaa atcattgttc actgaaaagc gttggatgtt ctttttcaaa ggtgtctaag
    80581 atctctcgta cttcatcagt agatttcgta ttcatcaact gaactcgcaa gtcatttgct
    80641 cctggaaatc ctttgatata gattttaaag aaacgatgta gtcctgtgat ggaacgcggg
    80701 atgatttctg aataatgatc ttgcagatct agttgcagac gaagtagtcc gatcagttct
    80761 ttttctgtat gttccctcgg ttctttttca aatgcataag gattcttgaa aatcccccgg
    80821 ccaatcatga tgccatctac gccatatttt tcggccaatt cttctcctgt ctggcgatct
    80881 gggatatctc cgttgatcgt gatcagtgtt tgcggagcga tttggtcacg taaagcaatg
    80941 attttaggga tgacttccca atgtgcatct actttactca tctctttacg cgtacgcaaa
    81001 tggacagaaa gattggctat atcttgatga agtaggtgac tgatccattc ttccatctca
    81061 gacatttccg tatagccgat acgcgtcttg acgctgacag gcaaaccgcc agctttagcc
    81121 gcttcaatca attgtgccgc tacttctggg cgaaggatca agccactgcc ttttccacgt
    81181 tctgctacat tagggactgg gcagcccatg ttgatatcga ttccttggaa gcccatttct
    81241 gccatagcaa tgctcatttc tctaaaaaat tctggcttgt ctccccaaat atgggcaacc
    81301 atcggctgtt catcttctgt aaaggtcaat cttcctcgta cactgtcttt tccatctgga
    81361 tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga catccggtgc tgctgcatgc
    81421 tttacgacat ggcgaaatac aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa
    81481 ggctttggca gctctttcca aaaattagta gtcatttatt tgttactctc cattctatca
    81541 agcagtgaag tcgcaatctt tctcaaaaac attgtcatta tactaggaaa caaaaaaaga
    81601 agcaaagtga acagcttaaa agatgtttct gattgagcaa aaaaagactg tgacaaaaat
    81661 cttatcacaa ttccttttcc ggaataaatg gtgttcaaaa gcttgcatct gcggtaataa
    81721 gtccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
    81781 cagaacagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
    81841 tagctcaaca aacgacaaaa tatgaggaac tattttcccg tttgtttctc taatactcag
    81901 gtcaaaacgc tgatttcaca acctcttttc taacttctca atcctcttcc tttattgatc
    81961 aaataccaag taacggcgta caaaacaacg atgaatagca caagtacgat catcgatgtt
    82021 gctactggca catcgattgt tccaaggaat ccgtaacgga aaccggagat catataaacg
    82081 atcggattga ctttcgaaac agcttgccaa aatggcggca acatagagat ggcatagaaa
    82141 acgccaccta gataagttaa cggctgcaag acaaatgtag gcacgatcga tacatcgtca
    82201 taagactgag caaaaatccc gttgagcagt cctgctaacg aaaacaagat tgccgtcatc
    82261 aataatgtaa tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa
    82321 atgatcgtta cgagagagcc gacgagaata ctgcgaccca ctccgccaat cacaaagccc
    82381 caaatgatca catgcgtagg aacaggcgct accaagattt cttcgatgtt tttctggaat
    82441 ttttgagaga agaaggacga agaaacgttg gcatacgaac tagtgatagc agacatcata
    82501 atcaaaccag gcacgatgaa ttccatatat gaaaaaccac ccatgtcacc gatccgacca
    82561 ccgatcattt ttccgaaaat aacaaagtac aaggatgtcg tgatcacagg cggaaccagt
    82621 gtttggaccc agatacgcaa atagcggttc gtttccttga tagctaaact ttttaatgca
    82681 gtaaaataaa gattaaacat ttttctcctc cgtatgatta ttttcttctg tgattttcaa
    82741 aaacagttct tctaaacgat tcgacttatt cctcatagaa agaactttga ttccttgtct
    82801 tgtcagctga tcgaagattc cgttcacccc ttgatttcgt tcaacttcga cagcgattgt
    82861 ctgttcatct tcaaatgtac aagcgtatcc ttcaatcaca ggtttggtct catatggcgc
    82921 taaatcaaag ataaatgttt caaattgcag tttggataga agctctttca tactagtatt
    82981 ctcgatcagt tcacctgatt ggatgatccc gatgttgcga caaagcattt cagcttcttc
    83041 taaataatgc gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc ggagaaactc
    83101 ccacatctcg cgtctcaatt caatatcgac tcctgctgtt ggttcatcca agatcagcaa
    83161 acgtggttca tgcatcaatg ccctagcaat catcagtctt cgcttcattc ccccggaaag
    83221 catacgtgca cggacatttc tcttttccca aagattggac tgcttcaaat atttttcgct
    83281 gcgtttcatc gcttctttgc gagagacacc gtagtagcct gcttgattca cgacgatttg
    83341 ctggaccgtt tcaaagggat tgaaattgaa ctcttgcggg actaatccaa tctgttgctt
    83401 tgctcggact agatccgtat ccagatcata accgaagaca ttcacttttc cagatgtttt
    83461 attgactaaa gaagtaatga ttccgatcgt tgtagacttc ccagctccat ttggtcctaa
    83521 tagcgcatag aaatctcctt ctttcacgga aagatcgatt cctcgcaatg cttcgacccc
    83581 acttccataa acctttttca aattatttat ttcaagcgca tttgccattt atttcctatc
    83641 tccttataga catgattctt tattgtatca tggaatacag cagatgaaaa aacaacttgg
    83701 ttccagcgaa aaatcaggtt gctgggctca ggaaaatctc ctaatgaaag acatcaatcg
    83761 gacgaagtca gacaaccttt tcagaaaaaa caaatcgaag ataaaggatt actattcata
    83821 tgtgcaacaa aaggagcgtg agacaaaagt aaagaacact tttgtctcac gctctaaaca
    83881 cgtataaacg gcggaagcaa aagccaagat ttttgatcca ataaatactt tcgatcactt
    83941 cgcctatccc aatacatgcc actgccgctt ttcatcaaat aacgcggctt ccgtttttgc
    84001 ggtttctact aaagtctctg ggtaatccaa aactttcagc aaggcaatcg catttcgggt
    84061 ccttgaaggt ccttctttta atttgaaatc aaaactgatt ccttttcctt ctgttacttg
    84121 ttcttcaaag tggacattct cacaataatt ctttaaaatc tctgtcaatt cgatatcatg
    84181 tgtggcgaca aatgctaaag atggatactc cgcaagccac cgaacgactg aagcggaaga
    84241 agcaatccgt tcgatcgtat ttgtcccttt taaaatctca tctacgaagc agtagcagcg
    84301 ttctttggaa gccacttggt ccaacaaacg tttgatcgat ttgatttctg aaacaaaata
    84361 actgtcgcct tcaaataaat catcttcgat tgccatggaa gtcaacacat gtccaggttg
    84421 cagggtaaac tgttctgcaa tcgctgtttg gatcgtctgt gctagaatac agctgatggc
    84481 tatacttttg acatacgtag attttcctga tgcattggaa cccgtgacta atgtattctt
    84541 ctgccagttg accggattca ctaccgcttc ttttaacaat ggatgataga tatctttcgc
    84601 tgtcacaccg ccctcagtaa actctggtcg gcaagtgatg ggcatataag tacggtaatt
    84661 caatattgca gcggctactt ctaattctcc cagcaattcc cacagcttga tggcttcagt
    84721 tgaatgcttt tctaatttag tgagaacgaa atgataggag ataaatggca gcatgaacat
    84781 gatgttgaca tattcaaaaa tcaactctgc ttcactccca ctcttcatac gaaatgaaaa
    84841 acctaattta gggatgtctc ttaatggttt agtttgctgt ttcagctctt cttgaagtgg
    84901 tgtcggcagt tttgctagct gattggcggc tgaaatcgtt gaaaccaaat aactcatgct
    84961 attcagctct gtttctagtg tttgtttctt cactagataa tagaccgtat tgaatactaa
    85021 cccaccgatt gccaaataga ttcctagtac ttgtcctaaa agaagcaaac ccaaacccac
    85081 cagcggaagc aagccaagaa aaataaataa acctacgcgt ccaatctttt tactttttcc
    85141 atccgctaga tattgttttg caaaattatg atcttttttt cctaaacgag cgaattgata
    85201 ttggattttt tctctcagtt gaggattttc ctgataaaaa gcaatcagtt tttctaactg
    85261 ctgatcttca ccaaaatcaa agctgcgtaa acgttgatac aacgcttctg aacctacact
    85321 agaataggta ctgttgattc cttcgaatac ttcgaacata tccaggtcat accaagtaag
    85381 atcatcgatt tcgctatccc atgaacggaa cttcttctcc gtgagccatg cttccttcaa
    85441 gctttcttct ttatcaaatc tcggttgata aggaattttt tcccatttgc tacgaactgt
    85501 ttcctttaat ttcaaacgtg aaaaaatgtc taaagcaact aataaaataa cagctgcaat
    85561 aataattaaa acgataaatt ctctttgacc catatccctc tccgttcctt tatttgttag
    85621 ctttagtata gcactgttgc gctatttgaa aagagggtaa aaaaacacct ttcaaaataa
    85681 aaggtgtttt tttcttgtat attacatatt tatctctcat ttatcaaaat tgacaaataa
    85741 tcatagggag gaattgttgt taattcttgt ttattctcca gtgcattgac aactgtccca
    85801 agggaaagga tagcctcagg tactattttt tcttgttctg ttggattagc taagcgaaga
    85861 acaaatcctt gttcatgaaa ataagaagga taaatcgatg atacaattaa gtcttcaggt
    85921 aattcaatca atgaaaattt acgaggaaca tcaagttcat tttgtaatgg ccaaatcttg
    85981 ttatctaacc tgtgaataaa tacattcagt ttctgctttt gataagaaat agattgttct
    86041 aaccgttttt ctgcctgttt tgccactttg aactcattaa atcttccttc ttctacatta
    86101 aatccaaaag tgactttata ctcacctatc tcttgagcta gcggtgtttc catcatgaca
    86161 tgcccttcat ttgtcgtatc tcctgaagct cgcccaggtc gccaagcaag attaggtttt
    86221 ccaagttccc ctgttgttga aaataaggtt atgattaatt gattttctat acgttcatac
    86281 tcttttaaac catctgcata aaacgtaaaa taatgatcat tttctgccac agagacactc
    86341 ttatcgaata tttctaaatt aacaggtttc tctaccatag tttctgacca attcggagga
    86401 atgacatcag gggtattttc aataaatccg ttttgaattt gtgcaattga tttttcagat
    86461 ttatctaaaa cattaattgc taagcgcata cgatgcgagt aaacagtatt atgaattttg
    86521 agtgtgcctt caacgatttg actttctccc agactgaacg atatttcata agatatttct
    86581 tccatttcgg gtttttcaga taaacgatct tttaaatctt taggaagttg agcttttccg
    86641 taaacaacta atgtttcttg taaagaatct ttatatactt tagctgtttc taattttaga
    86701 gatagttctg tatctccttc taatggtgaa taatcatacg tatcgccatc atttgctgaa
    86761 tcgatcaaat gaacgaaatc aagatattgt ctatctccaa ctatcaaatt caattgccca
    86821 tctttaaaag aaagtttata tttattgtta cttatctcag ttttgtctat ctcgattgct
    86881 gtttctaatt tttcattatt tttctcaaat tcaacaacag tgtatcctag tgctggcaca
    86941 tttacattta tttttatgtc aagttcataa taagctggtt catcaaaata ttcgaaacct
    87001 gtagcaatca tccgataagc atttttccgt tctggaaaat aaacttcttt ttcaatgata
    87061 ttatagggac tacctttgaa gtgtattttc tttgaatcag ctactacatg cacgattttc
    87121 gatccactat aattctcagt cgttgtgttg aaaacaagga cttcattcga ttttagcttt
    87181 aatgcatcag ccatccgttt tacaataagg ttttcgattc cgtcagctat ctcattagct
    87241 tctttgattc gatggaaaat atcttccgca acattatctg aaacacagcc accaatacta
    87301 tcatgagcct ggttttgcaa aatctttttc cataaccgac gaagtaggcc tttactgata
    87361 tggataccat ttctttctgc aatcagcatg agcggctcaa ctcgttttag aattttttgt
    87421 tccaatttaa aattttctat tttcattttt gttcttactg agccaataga acgatgaaca
    87481 cgagagtaaa ccggctgacg aagttcacct ttatattcag gtaattcttt tttgtttctg
    87541 ataacttcta cataagaagg ataatctgaa ataatattgt ggaatttact gtatttattc
    87601 aacttctcta cgacttgatc aaaatttaaa atcattgact tttgatctac acccgaaggt
    87661 actaaaatat ctttgttttt tccttgcata tgaagcattt caactgcttc atccaacttt
    87721 tcttttgcaa attcaggaat atctttaatt gcgtctaagg tcatcattcc agtcatataa
    87781 ccaaatggaa aattcatggc ataaacaaat ttatctccca atcctttcca gataaaataa
    87841 ggagatggaa ccatcgtttc aaagtttaat cctcgccaac acatgaaatt atcgatttct
    87901 acttgatgca aaagtgtagg caaattcgca ttgaacccaa acgtgtcagg caaataccct
    87961 accatcattg gatctccata tttaaggatc gtatcactga ttccaatcat taagtttctt
    88021 aaaattgatt cagcatccac taataaggca tctgtttgtg cataccacgg tcccacgaat
    88081 agttgtcctg ttttcatcag tcgttggata accttaagct tttgcggatt gatctccaaa
    88141 tactcatcga cgattgaagt ttgtccatct aaacaaaagt ttactttagg gttcctttcc
    88201 aattcatcta gaacttcagt aaagacctga tcagacaata cgatggcatc ttcatttgta
    88261 aaataccatt ctctgtccca gtgcgtgtgt tgaattatat gtgcatttac catatcagat
    88321 tctcctactt tccttcttct actgaattta cttgttgttc ttttttatac ttgatggatt
    88381 ttagtgcatt gaccattaac gctgtaacca ctcccccaat aacaattgat actaaatata
    88441 aaggtatatt cgtaacaaaa ggaattgcca taaaggttga aagcattgtt tcttgagtaa
    88501 caccaaaaaa tccatttaga ccacatgcta ctgcagtgcc caccacgatg cttggtatta
    88561 ctcgtaaagg atctttcacg gcaaacggga tcgtcccttc agtaatcccc acaaggccca
    88621 taatcatagc tgtctttgca ttatctcgtt ccgattcatc ataaagtttt ttgtttaaaa
    88681 ttgttgctaa aaacattcca aatggcggaa tagcaattcc taataacatg gctgtactag
    88741 gtccaaagct tccgcttgaa aaagaagcaa ccccaaaagc atatgcgatt ttattgatag
    88801 gtcctcccat atcaaacgcc atcattcctc ctaaaatagc cgacatcaaa gcagcatttg
    88861 ctcctgacat gccttctagc caattagtca gtcctgttgt cattgcttga ataggcgtag
    88921 ctagtacata ccataatatg attccaacaa ctaatgtagt aacaaaagga atcagcataa
    88981 ttgattttag actaataact tgcatcggta cttttacttt tttcaatgca ttagctaaat
    89041 agccacaagc taatccagca accattcctc caataaatcc cataccaaga ttttgacaca
    89101 tcatcccagt gataaaagct ggtgctagac cgggacggtc agcaattgaa tatgcgatat
    89161 aggctgaaat catcggtacg ataaactgta atcccgtctg accaatcatt cgcaggactc
    89221 cccagaatgt atccgtctgg tcccaaacac ctgtgccacc aaaaatgact gcaatagaag
    89281 ttagtaaccc tgctgctacg acaagcggga tcatatagga aaccccactt tggaaatgat
    89341 ttagaatttc tcttccttgt ttcttgaatt ccattagtaa ctcccccacg atatattttt
    89401 tatttgttaa ttaatgcaat tgcttcatca atcgtctctt caccatcttt tactacctct
    89461 gaggtcccta ctgttaaaat cggtttaccg tcaaagcgac ctcgttctga aacatttgta
    89521 tctactgcaa atatgacaac atctgcctcg gaaacatccg aaattgaaag tttgttttct
    89581 atccccatag ccccctgtgt ttctacttta atatctaacc ctttcttctt cgcaaacttt
    89641 tctaaattag cttttgccat ataagtatgg gcaatccctg cgatacatgc tgttactgct
    89701 actatttttg tcatatgaaa ttcctccttt taaaatgata gagcttttac caattctttt
    89761 ttatttctcg aatcttttat tcttttaaca aactcgtcat ccatcaatct tccagataat
    89821 tctgctaaaa tacgtaaatg attagtacct ttatcagtgt cttttattgc taacaaaaag
    89881 acgagatcaa ctggttgatc atctaatgcg ccccattcaa cagaccgctt taacttggct
    89941 acagccactg ttgattcaat tactgctagg cttttgccat gaggaatggc gatatttttc
    90001 ccaattcctg tggtcatatg gttttctctg tcaaatacgt ctttttgaaa ttgtttgtgg
    90061 tcagacacaa acccattagt ctctaacaat ttcgataatt tttcgactac acttttttta
    90121 tcagatgcat ttaaatctat cactatatta tttatattga ttttttcact gatcatttat
    90181 aagccttctt tcaatatata aactaatttt tcaaacgacg aactcgtttt taaatttgct
    90241 aataactcat gattcgaagc gatttcattg aatgtttcat aaattttatt caattcaggt
    90301 tttacttttt cattaagtgc aaaaaagaaa acagaatgaa caagtgtccc atcccaatca
    90361 attccttctg gattaacgta gacacagatt actggctttt ttataaaatc tacttcgata
    90421 tgcggcatag caaaatcatt catcgctgtt gaagatagtt tttctctttt gattgctccc
    90481 tctagtatcc cttctttcgc gtaattttct tttaccaatt ttttggtaat tatatttaaa
    90541 acgttcactt gattatcctt tatttggtgg gtaaaaaagc aaaatcgttt atctaataaa
    90601 tttagaaaag catcttctgt ttgttcattt aactgtagat actgttttac ttttctgaca
    90661 tctgccccta acatcaaagg actaacaaca ataacaggaa aatttgtatt ttcaatcgga
    90721 atcgtgctta taacaagttc atcagtgact tttctatctt gtagttcatt gatgcttagt
    90781 acatcttcta tctcaaccat atctgcaaaa ccgttattta ttctttgctc taataatttt
    90841 gatgttccat atccgctact acaaactaat atcactttcg ttttgtcagg tttgtaacga
    90901 tccaaaagtg attgaatatg gagtgttacg taagcgattt catcttcatt aaaatgtata
    90961 cagaatctct cttcgatttc ctctaataaa tctacactta tcataaatga ccttttaaaa
    91021 gaatatttga ttttgtccgt ataagggtta tagatattta ctcccaattt gagtctttta
    91081 actgctgcat tgagatgtaa tactagatta ttgatcaatt cttcatcagg ataaaattca
    91141 tttaatttaa ttcttactaa ctcttgtagc tcaacaccca tatctaaata ggaaagcttc
    91201 gtttgatcta agtcgtgatt catagcattt ttttcaatcg aagtaatgtg aatgtcaaca
    91261 taagcttgtt catctattgg cacaataatt gaaaatgctt tttcaatcat tttgattaaa
    91321 ataagtgtat tctttgagat ttcttcttca gaattattac tacgtgataa gaaattgctt
    91381 gtttcaatcc tttctatcgc aatagcaagg tggatagcta aagtttgaaa ttcataatct
    91441 gtaaatttca attgtgtctt attcatgtat cgattaagca attcggctac ttttttcaat
    91501 atgtccttat ttaagaaatt attcgcttca ccaatgaaat tgatattcaa gtctaagaga
    91561 tctgttttac tgttagatgc tgtaaagcca ccccaataat agttcacaac ttctgacatc
    91621 aaccttcttt tacttttttc atcagctttg atgcgcacgc catttcgatc tgaaactaag
    91681 gacatcccat gcttgtttag tctttttcta acatctttca ttgtgctttc taccgtcgac
    91741 ctgctcacgt ataattcgtc agcaagattt tgaatcttta gagaatcatt agtattcaat
    91801 aatttagcaa acacataagt tagacggtcc tccggagagt ctgcgatatt gattttatga
    91861 ctggataata aatttcgaag tgtttctttt tttccaataa gcgaaacgcc ttttttaggt
    91921 tgaaccaaca tttgtacatc tggcattttt tggattattt ctttcaaaat taaaatatct
    91981 ttgctaattg ttttcgtaga tacatataat tgttctgata attctttgta agttgtgtgc
    92041 tcattttcaa tcaaaaagta tagtattttc atttgccgtt cgctaaattc gatgcccatt
    92101 atcttcacct cctgaataca gcttaagtgt acaattatta tctctatttt catacagaag
    92161 aaagttcttg attaatgtag aagtttctaa tattcttgat tatttcaaat caataaaaaa
    92221 gcctgcaata tttatatcac aggctttttt ctctattaaa tatatataag ataatacatt
    92281 tttctactag ttctcactaa tcttgtataa ttaaaccctc cgcaacttga cggagatgga
    92341 gaagatgccc ccgtctaatc tagcggtcat ctctcctccc attccttcca ccaatttttt
    92401 tgacaggtaa agcccgattc cgctagattc cgtatttgtg cggctggtat tttcggtata
    92461 aaaacgctgg aagacttttt caacagaggt ttgcgttcct ggtttgatag gattggccac
    92521 tgttaatcga atgaatggat cctcttttga aaatgtcacc tttgcttgtc catctgcata
    92581 acgcaataaa ttctgtatga tattttgcag cactcgcagc aacatgagcg gatctgctaa
    92641 tacagttgta tttatcgagt gttcagcgaa cgctacctcg atatttttct cagaaaattg
    92701 ttcataaaaa gagaaaaata cttcttccac ctctttagaa aaagaaagcg tatggagatc
    92761 catttctttt tgcttcgtct ctattcgagc aagatcataa aactgatccg tcatactgag
    92821 taatcttgca acagattgtt tgatgatcgt taaatacttc tcccgtttct ccgtatcctc
    92881 tacctgttcc agtaattgta cataaccgtt aatagcagtc aaaggagttc tgagatcatg
    92941 agaaaggccg acgatcgata gtctgatgtt ttcttccatt tcttgaaatg cttgtccttt
    93001 ttgttcattt tcatccaacc atgtattcag tgcatctact aggtccatta gattcttttc
    93061 tcgaaattcg actgaaagac gaccaccata ttcttgtcgt tcagaaagag tggcgatttt
    93121 cttagttaat ctcttgattt ccctttgcag gaagaaaata aaacttccca taaagataac
    93181 aacgagaagc aacagccatt taatcaaatc taaaccccac tccccacacc gtatgaatat
    93241 acacagcatg tgcctttgct agcttccctc gaagattgga aatatgcata ttcactgttt
    93301 tctcactatc aaaatatggt tcttgccaaa ctgtttcata aagatttgct cgcgaaaata
    93361 cttttttcgg atgttctaaa aaaagcttca gcagatcgaa ttctcttcct gtcaaatgga
    93421 cagccacacc attcacattt acatccctcg actctttgtc cagtaagatt tccttgtaat
    93481 agatgcgttg cgacagatct tttacttgga cagaagtctg atgacgaagt tggatgccga
    93541 ttcgtaacaa aagttcttcg atatcaaatg gtttcgtcac atagtcatcc gctcctgctg
    93601 ccaacaagga aactttatct ctttgatcgc tttttgctga aagaacgatg atcggaacat
    93661 tcgatgtctg tcggatttct gctaacaatg cttcacctga aagccctgga agcatgagat
    93721 ctagaaggat caaatcgaca ggatatgtgc ttaacaataa ttttccttcc gtacctgaat
    93781 aagcatcaag aagtttgtat ctctgttcaa gaacctcctc cagtaaggaa tgaacagctt
    93841 catcgtcttc gataatcaaa atagtttcca gcaggaccac ctacttaatg tcagattttt
    93901 ggaaaataaa cattcctaga gctgatgata ccacgattgt tactagactg accagaatca
    93961 ttttcggtgc ttgcgaataa gttgcttgtt gcgcaaagtc cgtatacatg agccacgttt
    94021 ccgtaaacca ctccgttaga aattccaagc ctttgatttt tgcgaaaaca ccgggaatga
    94081 aggccgttcc catgatataa agaaaagaaa gaagcaaact gacactggta cttctagtga
    94141 tgaatgtcaa agccactgga acagataatg tccctaatgt cagcagaagc aatacaggaa
    94201 atacttttag aacataattt ccatagtttt tgattccctc aacttcacca aatccgaaag
    94261 caagtgtgta agcaagcatg gctagtcccg tgatcaaaac cacgccaagt gttgtgatca
    94321 aaagcgtaac gagaagtttc gacacataat aagtcattcg attccgtcct aaagataacg
    94381 tattgcgaat cgtaccgttt tgaaattctt ccccaatata aaagcacgca aatgctgcga
    94441 aagccagaag aatccatgca gccatttttt ctaccattaa aatccctgtt gcaccgttga
    94501 tgactgtatc cgttgcttgg tttaattcat tgccgtattt tgtatgcata taccattcgt
    94561 tccaagaata aaaaagcgga agtacgacca tcagtcctag tgagacccac attttacggc
    94621 tcttccatac ttttattttt tcagcagtta atagattaaa catgtggatt gcctccagtt
    94681 aattgtaaga aataatcttc cagtttttgc ccaactaggt gaataccatc cacaggaact
    94741 tttgcttcta ccaatgcttg attgatggct gctacttggt caattccttc aaaaagctgg
    94801 ataacacggc tagattgtac aaaataatcc ctataaccaa gagagtcgag aaccgttact
    94861 gcttgttccg tcgccgaagt gtcaattttt ataaattgac gagattcttg tgccaattct
    94921 tctttcgata attctttgat caagtgtcct tcatgcaaaa taccatagtg agtggcgatc
    94981 tgtgacaatt catctagcaa gtggctagaa agcaagacgg tgatgccccg ttcttttacc
    95041 aaacgttgga tgatttctcg catttctgcc atccctgctg gatccatccc attcgtcggt
    95101 tcatccaaaa tcaaaaattc tggattcgtg atcagtgcat ttgcaatagc taaacgctgg
    95161 cgcataccta aagagaaatt ctttgctttc tttttccctg tattttccaa tcgcatcatt
    95221 ttcagcaggt cttcgatttc tctatccgac acaccgccgt tcgctgcttg gatacgtagg
    95281 ttatctcttg cagttaattc tggataaaga gcaggggttt caatcgattg acccatacgt
    95341 cgtcttgctg cctgcaaatc cgtcgttcca aataattcga tcgaaccttc atcaatcgag
    95401 atcaaaccca taatagttct cataaaggta gacttacccg ctccattttc cccaatcaat
    95461 ccatagatca ttccacgttt gatttcgatt gatactttat ctaatgcttt ggcagcaccg
    95521 tattttttgg ttataccagt tgctttcaag accgtttcat ttccaaaatc catacttatc
    95581 cttctttctt attgatgagt gaagtatagt tgacgggtat aaagaaagca gccagaaatg
    95641 tataaagaaa gtgtaaattt tccaattgaa tgtcaaaaaa aacaatcgga caatacgtgt
    95701 ctttcctatt atcaggcagt ttgttatacg cttgtatcag tttcctgatt ctttcttttc
    95761 gaccaaagtc gtttctctgt attcctcttc actgaaaatt tttgcttctt tcagatcaag
    95821 cttgagtaat tttaccagac cggttataag gataactata gcaagactcc ctcccagaaa
    95881 aattagactg tctcgcagac gtggttgcgt tgtcttccaa tcaaagagcg caccaaacag
    95941 tgctaatcca agtggactgg ccaccatggt aatcgttgtt atcgttgaca taagtcgtcc
    96001 ttgtatatgc gttggaactt ctcgctgaag ccagaccata taaaaagcaa aagaaaacat
    96061 taatccttaa aaacggactt atgaaaaata atacatgccg tttgggattt acaattagct
    96121 accaaataag aggttatgac aaaagacttt tcacaatccc ttcttccgaa taaacggtgt
    96181 tctcctgcca actcctcggt cacaagtcac aatatttatc aaacatgaga agcaattttt
    96241 gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcag aagcggataa
    96301 ataatattgt ttgatgatac tttatctttt tcccgtttgt ttatagatcg aatgatatta
    96361 tagaccaaga atcctaccta ttaacatagc taaaaacaca aaaaaggcac tttccaaatt
    96421 gtggaaagtg ccagaaaagt taggattaag gtaactgcgc tcagcattgc ttcgcccagt
    96481 acgattctcg taactcgtga actcgaacga taatcgcaat taacgttttg agaactgtga
    96541 agctttacgg gctttcttaa gacctggttt tttcttcgta tcattgaata agtttgaaaa
    96601 accttgattt ataaaggttt tcccgtaaat atattttatt atatttgatt aaatttgaaa
    96661 tgtttgtgtt ctaaaatgtg ttctaattta aaggcagtat atttcgtatt ttagattaaa
    96721 tttttaggct cattcattct caaatccatt ctattcagtc aataaaaatt ttaaaaagac
    96781 tttataaaca aagatgttca agcgagcatc ttattttttt gtgcttaatt tgaaaggaga
    96841 acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt tgagtttggc
    96901 tgttcaagca attgttggag caattgctcc tacgattact tttgctgatg aaattaccca
    96961 tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg aaggaacttt
    97021 tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt acaacggctt
    97081 taaacaagat attttctgta ttgaaccagg agtcccgatt tacaatgagt tcactcctgg
    97141 ctatgagaaa aacccgttac cagatatgtc cgataaagcc aaattagttt ccgttttatg
    97201 gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga tctggcaaga
    97261 agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg taaatatcgc
    97321 agccattgag gcaaaaataa atcaagccat tgacgattat cagaagaaac caaccttcca
    97381 taatagtacg gctaaaacag tattagggca atcgactact gtgacggata cgaataatct
    97441 gaatttgtca gagttcgatg aggttgtgga aaatgccgca aacattgatt atcgtgtgaa
    97501 tggtaatcag ttgataatca ccccaaatgc caattcaaaa gaaagtggtg tgctgacgct
    97561 taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc aacagacttt
    97621 aatggccggg gcgattgata aacctaatac ctacacaatc aaaattgatg tggaaacgga
    97681 gggttcttta aagatcaaaa aagtcgataa agaatcgggt gatgttgtac caggaacggt
    97741 tttccattta gactttggaa aaactttacc tgcaaaagac gtgactactg acaaagaagg
    97801 cattgctaca ttggatgaga ttcctcatgg tacaaaagta accattaccg aaaaatcagt
    97861 accggcgcct tatacgattg ataccacccc catgactacc accattaaag caggcgaaac
    97921 catttacgtg acttcaaaaa acgcacggga aaaagggcaa atcattctgg ataaaagcgg
    97981 agttgaaaca gggagtgatc tttggaatga caattattcc ttagctggta atacgtttgc
    98041 cattcgcaaa gacagtccaa ccggtgaaat cgttcaagaa atgaccacgg atgaaaacgg
    98101 tcatgcggaa acaccaaaag agattgctaa tgcgttggaa ttgggaacct attatgtgac
    98161 cgaaactaaa gctagtcatg ggttcgtgaa tacgttcaaa cctgtaaaag tcgaattgaa
    98221 gtatgccaat caaaccgtag cccctgttac cagtaacgta aaagggcaaa atcaagaagt
    98281 cactggcgaa accactttga cgaaagaaga caaagatact ggggataaag cacaaggtaa
    98341 ggcagtcttt gagggtgctg aatacacctt gtttactgct aaggatggaa aagctgttaa
    98401 atggagtgag gcttttaaac cagagttagc gaagggaacg aaaggttctg atgaaacggt
    98461 cactttgact ttagacgaaa agaaccaagt tgccgttaaa catttagcta tcaacgagta
    98521 ttattggcaa gaaacaaaag cgcccgaagg atatagcctt gatgaaacaa agtatcctgt
    98581 atccatcaaa aaggtggatg atagcgaaaa aaatgccgtg attacccgaa atgttacggc
    98641 aaaagaacaa gtgattcgct ttggctttga tttctttaaa tttgctggat cagctgcggg
    98701 cactaccgaa actggcttca atgatctgac ctttaaagtg tcgccattgg aagggaccaa
    98761 tgaaatcaca ggtgccgaag atgaagcgac cacagcttat aacgagcaat tagggtttga
    98821 tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaagc
    98881 cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta aggaaaacaa
    98941 agaagacttt gtgaagagtg agtatgtctt taccattacc gaacaagacc aaaaacaacc
    99001 aattaaaacg gtgaccgttc cttacgagaa gctgacgaac aaagcatttt ctgttagttt
    99061 gaaccgttta atgctctatg atttgcctga ggaagaagat agtttaactt ctcttgcgac
    99121 ttggaaagac ggaaacaaag aattaacttc tctggattct accgagctag ttgataaatt
    99181 gagttacaat ctacatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt
    99241 ggatgcgaca aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg tgattgcaga
    99301 aacaagcgca acgttggcga acaaagagaa aacaggaact tggaaaattc agcataaatt
    99361 aaccgccgaa caagttttaa ataaaactat tgtcttattc aactatgtgt atgaaaataa
    99421 ggaagctttt gaagcaggcg ataagccagt agcgaaggat gttagcttga ataaccaagc
    99481 ccaaactgtc agttgtacag tggaacacca tgtttctatc caaacaaagg cccacctaga
    99541 aaacggttct cagaccttta ctcatggtga tgtggtggat atgtttgatg atgtgtcgat
    99601 tactcatgat gtactggatg gctcaaaaga agctttcgaa acaattctgt atgctttatt
    99661 accggatggt acaaacaaag aaatttggaa atctggcaag attgattatg aagtaaatga
    99721 taaagaattc accaaaactg tgcttgcgaa aaaagtagat accggaaaat atccagaagg
    99781 aaccaagttt acttttgcgg aaatcaatta cgataaagat gggaatatca atggaaaaca
    99841 caatgaagat ttgaaagaaa aatctcaaac cttaacacca aaagaagttc caaccatact
    99901 aagtacgccg aaacaaccgg aaacaccaac tgttccaagt ggctctcaag aatctagtcc
    99961 cacagtgaag accttccctc aaaccgggga gaaaaattcc aatgttttac tgttcattgg
   100021 ttttactttg atttttgcga cggcgggcta ttatttctgg aatcgccgga actaaggtga
   100081 tgagatgata caagtaaatc ctaagaacca acgtgcgcga ccgcctccta aaaaagtagt
   100141 cgcgctattt ttgtatccaa aactaacaat gaaaagagga aaagaagatg gaattgaaat
   100201 ttgtcgtgcc agatatggct gaaacctttg gaaagttaag ttatgccggt gaaggagaaa
   100261 ttataacaga aggatatgga cggaatacta cggtgattgg tcgcagttat catttgtatt
   100321 ccagtaagca acgggccgat gatattgagg tggtagtagc tgcggaagcc ggtgaaaagg
   100381 attttgatca agaccaaccg ctaaaagccg tgaatcccca tttggttgcc aaaggctatg
   100441 agattgagaa tcgtgggttt actgattacg tgttgtatgt tgatgattta gtgaaagcat
   100501 aggaggaaaa gaagatgaga ttaacagaag gtattgtcgt agattcaggt ttaacatttg
   100561 ggaagttgcg tttttccgca ttacgccgag aagtacggaa gcaaaatgag gatggaacaa
   100621 ttggtaatga ggtaaaagaa cgaacctata atttaaaatc ctctgcacaa gggcgaatga
   100681 ttcaagttag tatcccagct aatgtaccct tacgcgagtt tgcttatgat gcagaggtag
   100741 agttggtcaa tccaatcgtg gataccgttg ccaattatgt ttttcgagaa ggaactaccg
   100801 ttaattggtt cattaaggcg gatgatttag tcttgaagcg gcagccgaat caaggaaatc
   100861 ctacgaacca aaatgaaaga aagaaatagg tgaagattat ggaaattaat gttgaacata
   100921 ttttagattg tctcgatcaa tatggaaaag gcgagctgac tgaggaacaa ctcgttaaag
   100981 cattaacctc tgatgaaaaa atgtttctga ttatacatca aggtttactt ggagaaaata
   101041 gcgacagtga agaagactct gatgtactag actggttagg agaagaagag tctttcttta
   101101 tagttgtcga agtaaatgaa aacttatgtc ggcaggcaga atccattttg gaagaaattg
   101161 gtgtggagat gccggatgct attgaaggct ttttaaatca attggttgaa acgaaacaac
   101221 tcccctcctc gtagcagtaa atgactagca taactttttg aaattgccta atgaaatcct
   101281 attgggcttt ttcgattgga ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc
   101341 gcaggagatc agcacttggt ttatcacttt gttcttggct ggctactcgc tctgttcatc
   101401 ggttggatga gcgtctttta ttttcaagag tttagacaat ttgatatttc taagctatca
   101461 ctttctacta ttgaaatcgt ttggtctata aaagatttag tatgtctgct tggaagtctc
   101521 gctttttcag gagctatgat attgctttat attcactttt ttcttgatca ttggcgaagc
   101581 ttgtggcatc gacaaaagct agctcggatg attctagaga accattggta tgaagtgaaa
   101641 caaactcaaa gtgaaggctt tttcaaagat ttgaatagta gccgaaccag agagactatc
   101701 agttacttcc ccaaaatctg ttatcgaatg aaagatggat tactgtctat tcgcgttcaa
   101761 atttcacttg ggaaatacca agatcagctt ttaaaattgg aaaagaagtt agaaagcgga
   101821 ctgtattgtg agttagtaga aaaggaactc aaagactcct atgtggagta tactttactg
   101881 tatgatatga tcgccaatcg aattgggata gacgaagtag tggcagaaaa tgggactctg
   101941 cgattgatga aaaatcaagt atgggcttat gattctttac cccatatgtt aatcgctggt
   102001 ggcacgggtg gagggaagac ctatttcctc ctcaccatta tcgaagcact attgaagtca
   102061 gatgcagaat tgtttatcct cgatcccaaa aatgcagatt tagctgattt aggtacggtg
   102121 atgcctcatg tttattcaca aaaggaagaa atttctgctt gtgtggaaga tttctatgaa
   102181 cgtatgatgg ctcgtagcaa ggcaatgaaa gaaatgtcta actacaaaac aggagagaat
   102241 tatgcctatc ttggacttcc acccaacttt ttaatctttg atgaatacgt ggcttatatg
   102301 gagatgttaa cgacgaaaga aagtgcggtg attttgaata agctcaaaca aatcgtgatg
   102361 ttgggtcgtc aatctggttt ctttctgatt ttggcttgcc aaaggccgga tgcgaaatat
   102421 ttaggagacg gaatccgcga tcaattcaac tttcgcgtgg ctttgggtcg tatgagtgaa
   102481 ctcggatatt ccatgatgtt tggggaggtc gaaaaaaact tctttatgaa gcacatcaaa
   102541 ggtcgaggtt atgtggatac aggtggcagt gtgattagtg agttttatac gccacttgta
   102601 ccaaaagggt atgatttctt agattcaatt agtaaattag aaaagatgaa tgattgagtg
   102661 atcttcaata ctgactttat acttcaagta acgtataata cttttatata gtaggaagaa
   102721 aggggataat taatatggaa agtaatgatt cttatttaga gtatgcaaaa gcaaataagg
   102781 ataagtttat ttcttatatt atcaaaggga aatcccccga agttggacga gacgcagttt
   102841 ttatggcagg gagtcctggg gctggaaaat cagaggttgc cattggatta gctgagaatt
   102901 atgataacca tgtgattatt gatgcggatt actttcgcac ccagtttcct gattataatg
   102961 gaaaaaactc tagtctcttt caaaaagcat cttcttggtt ggtagagcag gcatttagat
   103021 atgtcttaga tcatggctat tcttttattc tggatgcaac ttttgcaata ttaagtgctg
   103081 aaaaaaatat ctcgcgggcg ttgaaaaaac attttcgagt gacgattttc tatgtttatc
   103141 aagaccctaa aatcgcttgg aaatttacac aagaacgtga aattgcagag ggacgtcatg
   103201 tgccgaaaaa ggcatttatt aacgcttttt tcaaatcaag agagaacatt gcaaaagtaa
   103261 agagtaaaca tccagatgta ttgcttcata tcataataaa agattatacg aataatattt
   103321 ccgaagttca ttatgctacg gataatctag aattattttt accactaggg tatagtagga
   103381 aagatttgga ggttcaatta catgactgag aaagaacaag taacgaaaat tgtaaagaaa
   103441 tacaataaga gcattgcgga tttatcggaa aatgctactg caaaagagtt caaaacggtg
   103501 attaaatatg tagccgatca ggccaatgaa aaacaacgga aattagttgg attaaataaa
   103561 aaatagtagg acaaattaat aatgtatgag ccaagagcaa aatgttcttg gcttttttct
   103621 ttgcaatgaa tgaggtaatg tatgagattt ttagatttat tcgccggtat tggtggcttt
   103681 cgtttaggta tggaacaggc gagccatcat tgtatcggtt tttgtgaaat tgataaattt
   103741 gctagacgta gttataaagc aattcatgat acaagtaagg aggtggaaat gcatgacatc
   103801 acaagtgtat cagatgagtt tattcaatcc ctcggaccag tggacatcct ttgcggcgga
   103861 tttccgtgcc aagctttttc aattgcggga aagcggcaaa gattttccga tactcgaggt
   103921 actttattct ttgaaatcgc tcggttcacc actcttctcc gacctaagct tttattcctt
   103981 gagaacgtcc gaggattgct caatcacgaa ggaggggcta cgttcgagac gatcctccga
   104041 acgctggatg gattggggta tgacgtggag tggtaggtgc ttaactcaaa ggcctacatt
   104101 ccccaaagcc gggagcggat attccttatc ggacattctc gagacgcatg taccgaacaa
   104161 gtatttccta tcattggacc gtcttcaaca tctgatcaaa acatcagaaa tttggtaaat
   104221 atcaatcctt ccaatcgagg aatgggtgga caagtctatg gttcagatgg cgtagcgcca
   104281 accttaacag gtgatgaagg aatcaaaatc gcactgccag caagggatgg aatctctgta
   104341 gcaggactat taccgggaaa gtttgaacaa ggtaatcgag tttatgaagt tactggaacc
   104401 gcccccacct tgtcaacgaa acaaggtggt acaaaaatca tgattcgaaa aaatgaaaca
   104461 gaatatcaag aggtgaagcc agaagacagt gtgaatctgg cttttcctaa ttccacaagt
   104521 cgccgaggtc gtctgggaaa acagagtgtt cataccttac taacaggaga ccaacaagcg
   104581 atcgtcacgg atcagtatca aattcggaag ctaacaccta gagagtgttg gcgacttcag
   104641 ggctttcccg attgggcttt tgatcgagat tctcaagtca attcagatag tcagttgtat
   104701 aagcaagccg gaaactctgt aacagttccg gtcatttttg atattgccag aagattaaaa
   104761 gagttggatg tacatggttt atgatgagtt gattggagaa atttattggg tgattggaaa
   104821 gattcagtca gacccagaat tagaagaaca attgcgacgg ttaaaatttg atattcgcaa
   104881 gaatggtgtc aaagtcgctg gcgatccata tttaatgaat gaggagacgg atgctcgaat
   104941 tgaaataaat caagtaattg cagaatttga gcggattgcg gaccaagcaa aagaaccaga
   105001 tattcgtcaa tacttgttag aaatgaaagc agagttggaa gtaaatggta ttacagatga
   105061 gtaacctaaa ccaacacaac tgactgttag gcgctgaccg cggaggattc gcgaagcaga
   105121 aatcctccgt ggtgctgtgc ctgttcatgg acggcgttag ccgaccatga tgtctcaccc
   105181 ccgtatctaa caggggggta caaaaacaca aaaaataagt aagcaaccat taagaatgct
   105241 tgttgtatca aagatagtaa gtaatttgta taggtgttaa ctcatttgaa aaagttaaca
   105301 ccttgaattt ggaggtgaga acttggcaca acggaattta gattatcggc tattaaaaga
   105361 ccgacgcaac gaatacggcg tttcacaaaa taaattggct acggcttgtg gacttagtcg
   105421 gccgtactta aatcagatag aaaatggtgg cgtaaccgca tctataaaaa ccatgaggaa
   105481 aatttttgac caactggaaa gctttaatcc cgatttgcct ttgacgttac tgtttgacta
   105541 tgtaaggatt cgctttccca caacggatgc acgaaaaatt attcaagaaa ttctccattt
   105601 gaaatttgag tatatgctcc atgaagatta tgccttttac tcctatcaag aacagtatgt
   105661 catgggtgat attgtggtga tgttgtctca tgaagaagat aaaggtgtgc ttttagaatt
   105721 aaaggggcga ggttgccgac aatttgaaac ttttttactc gctcaaaagc gtagctggta
   105781 cgactttttc gaggattgtc taaaggccgg tggcgtgatg aaacgcttgg acttggcaat
   105841 caatgatcga gtggggctat tggatatacc tgagctaacg aaaaaatgtc agaaggaaga
   105901 atgtatctcc ttgtttcgta cctttaaaag ttatcgttcc ggggaacttt taaaggctga
   105961 tgaaaaggac ggcatgggca ataccttata tatcggcagt ctcaagagtg aggtttattt
   106021 ctgtttgtat gaaaaagact atgaacaata tatcaagtta gggattccat tagaccaaac
   106081 tgagacgaaa aatcgttttg agattcgact gaaaaatgac cgtgcttttc atgcgattca
   106141 agatttgtta aaaggtcgta gcattgagag caccacgttt tccattatca atcgttactt
   106201 gcgatttgcg gataaagtag aaggtaaaag gcgaaccaac tggcccttga atgaacaatg
   106261 ggggcgtttt attggacgga atcggaaaga gattcaatta acttccgagc caaaaccgta
   106321 taccattgaa cgaaccttga attggctagg ccgacaagtc gcccctacgt ggaaaatggc
   106381 taaggagttg gatcgattga atcggacaac ctatatccag gatatggtac agaatgcacg
   106441 cttatcagac cgacataaaa agattttgga gcaacaaagt atggcaattg aaaatttgat
   106501 tatatgaaaa gaggaatcgt catgggaatt gtaaaagata tttttttggt atttggtggc
   106561 agtatgcttg gtgttactgt gatgtgcttg atgaatgcaa gtgcagcagc ggatcgagca
   106621 atggaaaaga aaggaaagtg acaagatgaa tttcggacaa aatttatgtg cgactcgttc
   106681 cttagtgaaa agctaaggca ctaataaaag aacgaaaaag tttggttatt aggagaactg
   106741 ataatctgac aggtggaatg atgaagtaac gctctgaaac gcctgccctg agcctccgac
   106801 aagcattgta tgaggaaact cagaatgttt gaagctcggt aaagacggct gaaagatacc
   106861 gtaatagttg atgaacatat atcttctatc gaaaactatc cagaggtggg tagcgtatcc
   106921 aacaggtcgg gggtctataa aatatctatg gttagaatgt tctataagga actgacgaaa
   106981 cagcgaatgt acgggtctaa atttgaactt tgtagaaatg caaaggtacg agaaatcgta
   107041 gctggtgtgg caaagtaaga atataggtta tgaaatgcca tataacgtta caggcggtat
   107101 caagccagca ggcttatagc tagtgaccta aggatatata tgtatagata agattatcgg
   107161 aacgaggaaa ggtattgggt tctcatcaag agaataaact gacgaagaac aataagcagt
   107221 tgaactaatg ctgaaaagca gagggcgaac ctaagaaatt cttgtaatga gaatggagga
   107281 atgccctcaa gtcgtgtata tattagaaaa gtattatgtt gtttcaataa ggattacgag
   107341 taagataaaa aagctcactc aatttcaagt aaaggagtga agccttatgc ctaaaaataa
   107401 aaatgaaaca ttgtgtgtag aagatttaag acacgctgaa tattacgaaa tgcaaaacac
   107461 ctttgatgat ttatatgcca aaagtaagaa tggagatatt ttcactcatc ttatggatat
   107521 tatcctatcg agagaaaata tattactggc atatcgaaat atcaaagcaa atgcaggcag
   107581 taaaacagca ggaacagacg gtacaataat taaagatatt ggcaaattgc cagcagaaac
   107641 agttgtcaaa aaagtaagat acattgttgc aggcagtcca catgggtatc gacctaagcc
   107701 agtgaggcga aaagaaatac caaaaccaaa tggtaaaacc agacctctag gtattccttg
   107761 tatgtgggac aggcttatac aacaatgtat aaaacaagta ttagaaccaa tctgcgaagc
   107821 taaatttagt gaaaacagct atggttttag acctaatagg tcagttgaaa atgcaataaa
   107881 agcaacctat aaccgcctac aaatcagcca gctacattat gtgattgaat ttgatattaa
   107941 gggtttcttt gacaacgtga accattccaa gctgataaaa cagatatggg ctatgggaat
   108001 acgggacaag cacttgatat tcattttaaa aaggatatta aaagctccaa tcaaaatgac
   108061 taacggtaca ataacatatc cagagaaagg aactccgcaa gggggaataa tatccccact
   108121 actagcaaac attgttctta atgagttaga ccattgggta gaaagccaat ggcaggaaaa
   108181 tcctgttaca aaaaactatg tggttcatat caataaaagc ggtagcccat gtaaaagcaa
   108241 cgcttacaaa gaaatgaaga aaacaaagtt aaaggaaatg tatatggttc gttatgcaga
   108301 tgattttaga gttttctgcc gctacaagga aagtgctgaa aaagccaaaa tagcaatcac
   108361 tcaatggatt gaacaacgat taaaattgga agtatcacaa gaaaaaacaa gaattgtaaa
   108421 tgttagaaaa agatactccg actttcttgg tttcaagatt aaaatgatac cgagaagaaa
   108481 gaagttagtt gttaaatcac atataagtga taaacaattc aaaaaccaaa aagacaagct
   108541 ggtatcacag gcaaagaaaa ttgcttctcc ctcaaaaggg aaaacggaat tagaagaaac
   108601 aagactttat aattcaatgg tattaggtat gcaaaattat tatcagattg caacgaatgt
   108661 aaatctggat tgtatgaaac tgaatagagc tgtaatggct atcttcacta acagattagg
   108721 tacaaacaaa aggggcagat tgagaaaaac aggcagacaa cttacgacaa aggaggcaac
   108781 aagatatggt aaatctaaaa tgcttcggta tgttgctggc gagggcgaac ctattttccc
   108841 tgttggatat atccaacaca gaaaaccaat ggcaaaggtg tatttagcga attgctacac
   108901 tctagaggga agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca
   108961 gttaatgaaa ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct
   109021 attttctgct caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga
   109081 aatacattgt catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct
   109141 tattttggta aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca
   109201 aaaatatata gctctattga agctaaataa agtccagctc ataagactaa ataaatatag
   109261 agtattagct ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca
   109321 atataaacac gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg
   109381 aaaagtttga gatatttata atatcaggaa cttacctatc catatatcaa tggtttttaa
   109441 ccaatgccca atccttagtc ttactggcaa ttgtggtcat tggtttgttt ttaggattta
   109501 agcgggagtt ttccaaactg attggctttt tggtgattgc tttgattgcg gtcggattgg
   109561 tctttaatgc ttctggggtg aaggatgtct tactcagttt gtttaaccga attgtcggtg
   109621 cgtaaggggg aagcacgatg gaacagatgc gtgtttatat tgcgaacctc gggaaatata
   109681 acgaaggtga attggtgggg gcgtggttta ccccacctgt cgattttgat gaagtgaaag
   109741 aacgaattgg gttaaatgac gaatacgagg aatatgcaat tcatgattat gaacttccct
   109801 ttgagattga tgagtatacg ccaattgagg aaattaatcg gttgtgtggc ttagcggagg
   109861 aacttgaagg aacgccagtt ggtgaagttg cttctgagat tcaacatgct ttcttcaatt
   109921 cttttgagga gatggtagag catgtggatg atattatcta ttatccagat tgcgatgata
   109981 tgagtgatgt agcatatttc ttgattgtag aagctggtga ttttggagaa gtaccagaac
   110041 acttgaagac gtatattgat tttgatgcgt gtggtagaga cttggagatt ggaggcaatt
   110101 atttggtaac gagtcgaggt gtttttgagt atccatatta acaaaattat tgaagggtag
   110161 ttgatttcta aactatcctt ctttttaaag gaatgatttg atgaagaaaa taaaaagcta
   110221 caccagtatt tgggcagttg aaaaggtcat ttatgcaatt aatgactttc aactgccttt
   110281 tcctgtgact tttaaccaga tggcttggtt tgttttatcc ttgctgtttg tcatcgtatt
   110341 tgcccatgtg cctccactat ctatgataga gggggccttt ttaaaatact taggtatacc
   110401 ggtggcagtt acttggttca tgtcgcaaaa aacgtttgac ggtaagaaac cattgggatt
   110461 tttacgatct tttattagct atcacttacg atttaaggtc actttcgcag gaaagaaagt
   110521 caaagaacag aagaaacgat gggacgaacc cattactctg gtaaggagtg tgaactatgt
   110581 acccgattaa gtatattgaa aataatcttg tctttaatca agagggagaa tgttttgcct
   110641 actatgagtt aataccttac aattactcct ttttgtcacc tgaacagaaa tatcaagtcc
   110701 acgataactt tcgtcaattg attgcgcaaa atcgtgaagg caagattcat gccttacaaa
   110761 ttgccacaga aagtagtatt cgtgccacgc aagaacgatc aaaaaaggaa attaccgggc
   110821 gattaacaga agtagcgaaa caacgaattg atttacagac agaggcatta gtatccatga
   110881 ttggcgatag tcagattgat tatcgctttt ttattggctt caaactgatt gcgacagatg
   110941 aagaggtcaa tctaaagaat ctcaagaaat ctttcttctc cggctttcaa gaatttgtct
   111001 atggagtgaa tcatcatttg atgggcgatt ttgtttcctt atccaatgaa gaaattcgcc
   111061 gatataccaa gcttgaaaaa ctaatggaaa gtaaattggc tcgaagattt aaagtcagac
   111121 gagtcacacc gagtgatctg atgtatttga ttgaacatat ctatggcgaa aagggtacac
   111181 catttgaaga atatgaattt cagctaccaa agaaaaagct gaagtcggaa actttggtga
   111241 aacgttacga cttattacgt cccagtcgtt gtttgattga agaaaagccc cggtacttat
   111301 gcatggaaca tgagaatcat gaatcatatg tagcgtactt aacaattaat acaattgtgg
   111361 gggaaatgga gtttccctcc tcagaacttt tctattacca gcaacaacaa tttacattcc
   111421 caattgacac tagtatgaat gtggagattg tgacgaacaa aaaggcactt gctaccgttc
   111481 gcaataagaa aaaggaatta aaagacttgg acaaccatgc ctatcaatct gacaatgaaa
   111541 caaattccaa tgtcttagat gcattggatt ccgtagatga attggaaact accttggatc
   111601 agtccaaaga atccatgtac aaactgagct atgtggttcg agtaagtgcc gaatcagtag
   111661 atgaactaaa gcgccggtgt gatgaagtgc tagattttta tgatgatacc aatgtaaaac
   111721 tagtccggcc ttttggcgat atgatggggc tacatgaaga attcttacct tctagtaagc
   111781 gatacatgaa tgattatatc cagtatgtta cgtctgattt tctcgccgga cttggttttg
   111841 gcgccactca gatgttaggg gagttggaag gcatttactt tggttataac gtagatacgg
   111901 ggcgcaatgt ttacctgaaa ccggcattgg cctcacaagg ggtcaaggat tcagttacca
   111961 atgcgctagc agcggcattc ctcggttcac tcggtggcgg aaagtccttt agtaacaatc
   112021 tcttagttta ttacgccgtc ctttttggtg gacaagctgt gattgtcgat ccaaaagggg
   112081 aacggggtgg ctggaaagaa accttgccag aaattgcgga tgaaatcaat attctcaact
   112141 taactagtaa gccaaaaaat caaggattac ttgatcctta tgtgattatg gagaaaaaga
   112201 aggattctga aagtctagca attgatattc tgaccttttt aacaggaatt tctagtcgag
   112261 atggggagaa gtttccagtg ttgcgtaagg ccattcggac ggttacccaa agcaatcaac
   112321 ggggattatt acaagtaatt aatgagcttc gaaaagaagg ctcaccagtt gcggaaaata
   112381 tcgcggatca catcgagtcc tttaccgatt atgattttgc ccatttgctt ttctcagatg
   112441 gttcgattac ccagtcgatt agtttagata ggcagttaaa tatcattcaa gtagcggatt
   112501 tggttttacc agatgcagaa accacttttg aagaatatac caccatggaa ttgcttagtg
   112561 tttccatgct aatcgtcatt tccacttttg ccttggactt tatccatagt aatcgagaaa
   112621 tcttcaaaat tgtagactta gatgaggcgt ggagttttct tcaagtagcg caagggaaaa
   112681 cattatcgaa taagctcgtt cgtgctgggc gggctatgaa tgccggggtt tactttgtca
   112741 cgcaaaatag tgatgattta ctggatgaac gcttgaagaa taatatcggg ttgaagtttg
   112801 cgtttcgcag taccgatatt catgaaatca agaaaacctt ggaattcttt ggcttggatc
   112861 gagaagatga aagcaatcaa aaacgcctac gagatttgga aaatggccaa tgcttgatgc
   112921 aggatttgta tggtcgggta ggcgtggttc aggtacatcc agtctttgaa gaactcttcc
   112981 atgcctttga tacccgtcca cctgttcaag agagaatgga gtgaggggat gaaaaagaaa
   113041 ctttttcgga ttgtgggaat cgtttttatt agcatactca ctctgatagt tctattgagt
   113101 ttagttggta cggtagccga ggcgacaggg ctagttgatg atacagttga agcaggaaat
   113161 ctatattcca aatattcact gaacaactat caactagatt tctttgtgga tagctcttgg
   113221 gattggttgc cttggaattg gggtgatggc ttagggaaaa gcgtgatgta tggtctttat
   113281 gccattacta atttcatttg gacggtgagt ttgtatttgt cgaacgccac gggttatgtg
   113341 gttcaagagg cttacaaatt agatttcatt tcggatacgg ctgaaagtat tggaaagaat
   113401 atccaaacat tggctggtat tactgaaaat ggactccaga cctccggatt ttattttggg
   113461 tttctattat tgatgatttt agctctgggg gtgtatgtgg cgtataccgg attattaaaa
   113521 cgagaaacca cgaaagcagt tcgagccgtg attaattttg tgatgatttt tctactatcg
   113581 ggttcgttca ttgcttatgc gcctacctat atcacaaaaa tcaatgactt tagctcagat
   113641 gttagtgaag cggcccttac ccttggaacc gagatcgttg tacccaattc agaaagccaa
   113701 ggaaaagata gtgtggattt gattcgggat agtttgtttt cgattcaagt ccaacaacct
   113761 tggttactac tgcaatttga tgattccaac atagaagaaa ttggtgaaga ccgggtcaac
   113821 aagattctat ctgtgagtcc agatgaaaac aaaggcaaag atcgggaaga agcggtcaag
   113881 gcagagattg aagacaatga caatgcgaat ctcagtatta ccaagaccat gaaccgttta
   113941 gggaccgtgg tctttttggt attgttcaat attggcattt ccttctttgt ttttcttcta
   114001 acagggatta tgttgttctc gcaaattctc ttcattatct ttgcgatgtt tctgccaatt
   114061 agtttcttat tgtccatgtt gccgacttat gaaagtttag gaaagaaggc gattattcgt
   114121 ttattcaata cgattatgat gcgagcgggt gttactttgg tgattacgac tgcatttagc
   114181 atctccacga tgtttttcaa tatttcggca acctatccat ttttcatggt tgcttttctg
   114241 caaattgtga cctttgccgg tatctatttc aaattgggcg acattatgag tatgttcaac
   114301 ctccagagta atgatagcca gtcaatggga agacgcgtca tgcgaaaacc gcaaatgtta
   114361 atgaatcgaa aattgcggca actcaatcga aatgtgggac gagctttggc ttttggtggt
   114421 ggtgcagcag tcggaaataa gttggcaaag gaacaatcaa aatccaaatt taagccatcc
   114481 ggttcttctc tacgaaagaa ttcacgatta ccgaacgatc acgaaatact atctgactta
   114541 actaaggaga atccagtttc aaataataag aaacaatccc gaatgaattt ggctgggaga
   114601 aaaatcggca aagttttgga tacccaagct ttggtgaaag ataagactaa acaagtaaag
   114661 gatcaggtaa ggaatacgcc aacgaatttg aaatacaatc tccataaagg aatcgaaaag
   114721 tcgaaaaaag caccagaaga attcaagcgc ggactgttcc aagaaaaagc ggatcgagtg
   114781 gatttgcgag aaaaacaacg acaacgaaga aatgcgaaaa tggacgaaaa acgcaagata
   114841 ctgagtgaag caacaaatcg tcagggaaaa agaagggcga atattccaat agataaagca
   114901 gctcaacctc agaaagatat gatgctgaaa aaagaagcac caaagcggat tgtgagagca
   114961 aatctgaatc ccgaaatcaa acgaaaacta actagtcaag aaatcatttc aaaagggaaa
   115021 agccaatcag caagtaagaa aaattctttc caacaagtga aacagaggtc cactctgcca
   115081 aagcagacca atcaaaaagt acaaaaaaga atgaaccatc gtccaaaatc aaggtcaggt
   115141 gagaaaaaat gaacttgaag aaagtgggtg ttatttcatt aacttttctg accatctctt
   115201 ttatgggtat tttgatgtgt gtgggtttgt tgtttggtga agacagtgag ggaaatggta
   115261 gttctggcac ttctcctggc ggaactagtg tctcagaaga agtattgaaa caccgtgcaa
   115321 tggtggaaaa gtatgccaaa gaatcggata taacagagta tattccaatt cttttggcga
   115381 ttattcaagt ggaatctggt ggcacaatgg aagacgttat gcaaagttct gagtcagccg
   115441 gattaccacc taattcatta agtaccgagg catccatcaa acaaggctgt ctctattttg
   115501 cgtctttggt taagcgttcc aaagaactag ggtgtgatca agatagtatt attcaggcct
   115561 ataactatgg tggcgggtat ctagatcatg tcgggaaaaa cgggaagaag catagttttg
   115621 ctttagctga atctttttca aaggaaaaat caggtggtca aaaagtcgat taccccaacc
   115681 caatcgcgat taaggaaaat ggtgggtggc ggtataatta tggcaatatg ttttactgtt
   115741 tattagtgaa gcaataccta actacaactc agtttgatga caaaatagtc caagaaattt
   115801 ttgatgaggc atttaagtat gaaggaacgg cctatgtgtt tggtggctca agtccagaaa
   115861 cggggtttga ttgtagtggc ttgacccaat ggtgttatgc caaagttgac ctgaaacttc
   115921 ctcgggtggc tcaggatcaa tatgatgcca tgactcatat tgatctaaaa gatgccaaac
   115981 caggtgattt agttttcttt cattcaacct atgatgccgg tacttatgtg acccatgtgg
   116041 gcatttacgc aggggagaat cgcatgtttc atgcaggaga tccagtgggg tggacgaatc
   116101 taacggaaaa ctattggcgg cagcatctta tcggagctgg ccgatacaaa taagagaaaa
   116161 gaggaaaaag aatgaagata aaaattgaac ggaatcaaaa ggaaaagagc ccaaaaaaga
   116221 aaaaagaacg agttgtatcc gtgggtaagc atcgaaaaat ggtgcttgcc ctttggcttt
   116281 tattgtcttg tagtcttgct tttggcgttt ataaaaattt caccgccatt gatcaacata
   116341 caactcatga aaaagtggtt ataaaagaga aggtagtgaa tacttctggc gttgaaagtt
   116401 ttacagagga ttttgcgaaa gaatattttt catggaaaaa taacaaagaa gcaattgaaa
   116461 agagaatgag taatctcgaa cagtacctta cagaaaacgg tctcgcttta atccaagata
   116521 tggttcgagc agatattccc acgagttccg aagttcaatc tgtaaaaatt ctagatgttg
   116581 aaaaacactc ggaagaattt gtcgtttcat ttttagtgga gcaacatatt acagagggaa
   116641 aaaagacgca atcaatttct tctgcatatc gagtgacaat ctttgaagat gaaaacagga
   116701 atcatttagt aactagtttg ccaacaatga ttggaaagcc agacaaggca aaatatgaag
   116761 caaaacaagt agaaaacgat tcagaaattg atgccaaaac aacggaagaa atcaccgatt
   116821 ttttagaaac attttttaaa ctttatccat cagcttctga aaaagaattg gagtattatg
   116881 ttgaaaatgg tgcgatgcga ccaatcaata ttgacttaaa gttttcggag ttagagaatc
   116941 cagtttatct acaaaacgga aaaaattttc tagtgagtac aaagataaaa taccttgata
   117001 atgtatcaaa aaccactagc tactttcagt acaaattagt attacaaaaa aatggaaact
   117061 ggaaaatcgt tgatgttaaa taataatgac tcaatctaat tttattagaa gttataagta
   117121 gtgtaactta tttcaggcag aagtcgccca tcctctatag gtgggtgatg aatgccattc
   117181 ggtaactcga aaaccgacaa ttaagacata gtgccttttt attttaatcc atgtcataat
   117241 cagtctatat aggaaatggt tgatatacca tggaattaga tacaaataat cattcagttt
   117301 ttcttcttgg ttaccccctt attttagtag tgaaacattg taaacatgtc attgatgatg
   117361 taatgtcagc ttatgcaaaa actgcctttg aaagaatttc agaatcacat cacattacct
   117421 tagatgaatg aaatcgtgat aaagaatact ttcatattat gctcaaagca caacctaaaa
   117481 ccgaattaac aaaattcagc aatgcctaag ctcacgtctg ataaaatgag actttctaag
   117541 ggtcaaaaat ttcctttgaa tacgattatt gcttagataa aagtaaaagc cttagaacta
   117601 cggggatagc ttggttaata agagatatcg ttgttagtaa agacataggc ttgcaagtat
   117661 gctctattcc caagaatctc ccccttcaag caccaaggat cgttaaattc agctaagtgg
   117721 caagtaattc actagacgat aattatctcc atatttcaat ctcagctttt ccatttttca
   117781 tagcaaattc cgttattttt atagcatcta aaagattcag cttttccact gatctttcac
   117841 cacttttcag cttttttatt gtgcttaaat tgataccagt accttttgat attgaattgg
   117901 aagtaaaatc attaagggtt ttttttatta aattcatatc cgcaattcca gcgttcataa
   117961 gaatgaagat tctcctctca tagactactt tgaaaatggt actacagtgg ctgtttatat
   118021 atttcagtag gttactgtag tggtcatctt attataatta gaaaaggaac ctaaatcaaa
   118081 aatgctaaag aaaattttca tagtattaag cctctcatga ctaaagccac ggaattcttg
   118141 ataaaagagt atacttgcaa gcctaaacag cggtatctct tattaaccaa gctatccccg
   118201 ttgttcctaa ggttcttgat gttttctaag gcaatgcttg tattcatagc ccttggtcaa
   118261 aatattgata ctatcgttga cgtttcgatt atgatgtata cggcaaatag gacaagtcca
   118321 ttctcgaatt gtgagtgact tcttaccatc tgtgtgtcta cattctgaac caatttgact
   118381 agacggaaat catttatcta tcttaatgat tttttgacag aagtatcgaa aatctttaca
   118441 taatctgggt gatgtattct tatgcagatg agagcgttgg ctccgtattc ataaccagta
   118501 gcctctaaaa cgtagtccat cggtgggata cctagcttgc ggttgccagc ggctttcgca
   118561 gtcttcttgt gatcaaatca agcatctcat tgagcactag tattttgaag ataatacttg
   118621 agtttttcgg cttttagaag ttcacttcat cttccatcat ccatggaaat cccactcacc
   118681 catacgcagg cctccaaaaa ttcttcaaca gtggtttttt cagacatttt tctgaatacc
   118741 tccttgtttt ttgatacgcc agatagtcag gaaaaaggca acaaaacaca aaacaaagct
   118801 gcctaaaaag gtaatcttca ttccatataa aaagacatcc ggccgatttg cgatatagtt
   118861 agttacttgc tggcccatct tagcagacat cgcttgatat aagaccgtcg tagaaagcga
   118921 aatcccgctg accatcccca aatttcgggc aaaggaattc atcgagcctg ccagtcccaa
   118981 gttttcgata gtgaccgagc tcataaccat cgtattattg ggggactgga acaaggcatt
   119041 gccggcaccc atgatggcag tagcgacaat gtatagccac aaaggagagt ccactgacaa
   119101 aaacatatac atgacctggg ttaccgacaa taagcccaag cctactaata ccaaaatctc
   119161 cgggccaacc cggtcggtca ggtagccgct taaaggcgcc ccgacgacca tcatcaaagg
   119221 aaagaccatc atcaacagcc cagcatagct aggactcaac ccgcgagcat tttgcagata
   119281 aaaaggaatc actacattga caaagaagtt ggaagaaaaa atcaacaagg ctgtaatcaa
   119341 gctcatggta aaaatcttgt tcttaaaaat accgaaagaa atcaatggag aggctgcttt
   119401 cttttcaact tttaaaaagc tgacaaaggc aatcactgaa attacgaaca gtacaagggg
   119461 gactggatgg aaaaagccac tttcttgccc caaaaagatt gctccaaaaa agctgataat
   119521 cgtagccgca atggcagcaa agccccctcc atcgattgta tggccgctca tggtgatatc
   119581 ttttggtaag aatttttcag caatcaacat cgttacgatt cccaccggta cattgatcca
   119641 aaaaatgtac gcccaagaaa attgggacaa aatcaagccg ccaagacctg gtccagcaat
   119701 cgagcctaat gaaacaaacg cacctattgc tcccaatgcc cgaccacgtt catgtatggg
   119761 aaagacttcc gtgatgattc cggcatttgt agccatagtc atactagcac caatggcttg
   119821 taccacccgt gcgaacaata aaatacctag cgaatgattg aacccacaca acagcgatcc
   119881 caagacaaag atcagcgtac caatccgaaa tactttgatt ttcccacggc tatcgccaat
   119941 tttaccaaac agcaacaaac aagaacagac aatcatcaga taaacggaca ctacccattc
   120001 cgcttgattc attggtatgc ccatatcatc tgagatggta ggtagtgcaa tattgacaat
   120061 actgccatcc aatgtggaca tgaaggtaaa catagatata gcaatcaaaa tccaccagcg
   120121 atttttttgg acttttgcat cttcttgata actatacata ataggatttc ctttctaatg
   120181 atcgtgtagc taagaacaat ataacctatg tctactgccc aacaacttct cgcgactcta
   120241 gccataggaa gcttgagtaa tttcgatttg atatcctcat gtaaccatgt aagttggaga
   120301 tgctttattt gaacttactc tatagtaact agtgataatt cgctttactt tcttctagca
   120361 atttctaaaa gtcgacttca ttgttcttca gattaatagt gtgagctatc tcgtgagacg
   120421 taagcaaatt tccattctac tttttcccaa ttttttcata tttatggtgt tgcacctatt
   120481 tgttttccaa tttgcttttc aaatacattg ctatgatatt caattccata ggattatact
   120541 aaatatcaat agtatgaata atatattata gatgtttata tatcactgtt aattattatc
   120601 cacaataatt aatgattacc tcgatatttg tcaatcaaag gttgaaacaa tttattttca
   120661 gcatcttttc tagcttgagc tgcactctta atagaatcat acgatcctaa atagtacctc
   120721 ttttttttta aagttatata cgcaatctat ttctctttag aacgataaac tcccttaaca
   120781 ccacttgtat tatttttcgg taattttgac gttagacact ctattactgt cccttcaaca
   120841 catgtccttg tttcttgcct ttttaaacat ccacaacttt gagtatgtcc aaaaaataag
   120901 ttactataac ttatctcaca catactgcca cactcacaat gacaaaccca ttttgttttc
   120961 catgaacagt tattgtccaa aggatacttt gcagtaagta atccgaattt tttattttca
   121021 atgtgatgct tttttgattt caaacatcca cagcttttta catcaccttt tagaagtcgc
   121081 tttgccgata ctaacttttt tctcccacac agacattgac attcccaaac aacagttcct
   121141 tcacttcgca gtttagttgg cttgattgca actaacttac caaaagtttt atttgtcaaa
   121201 tttttatatt tatgcctatt tttataattt ccacatccac aacttttggt tctacctgat
   121261 agtaagtttg atcgatatac aatcgtttca ttcccacagt cacataggca acgatatttt
   121321 ttatatccat tatgagtaat ctcctcaaca acagtaaggt gaccatatct ttccttcact
   121381 gcacctaccc tcccaagaaa gaagtaccat ataatttcaa ttaactttta ataaatcaga
   121441 aaaaaatatt tccaattttt cttagtctga ttttatgact aatttacaat acaagactga
   121501 acgatacgta cacgaaatcc tcaagataga taaattgttt tcatctagtg aaacctgctc
   121561 aaaatatgag cataaagacg gcaataatac actcgatatt cgataatgga cttgttttat
   121621 ttgtcaaacc catcgtaatc gagatatcaa ttccagtctc aatatcctga tcaaaggtct
   121681 aagaatccaa ccattttctt gttactttgt cagattgatt acctttttta gtctccgaat
   121741 aataaggttt tcggatcaac taacatgctc tgagaaagac tactatcttt gcaaatttcc
   121801 aaatgaaggt gtgggccagt actattccca gtacttccta catagccaat tatttgtcct
   121861 tgcaaaacat tatctccagt ttttactagg tattgttgct ggtgagcata taacgcggtt
   121921 gatccatctt cgtgttgtat caccacgtaa tttccccaag agtagtgaaa ctctgcttta
   121981 acaaccttac cagctttaac tgcatgaatc ggctcaccct gtgtagctgc catatcaagt
   122041 cctcggtgaa attcatttcc acgatttcca aacaaactgg ttattgaata attatgaata
   122101 ggcacttggt atccagtctt actcatttgt ggacctttaa cttctttatc atagttggtt
   122161 aaatcgtagg ttttaattaa ttcatttagc ttcaaatagt attgtgtatc tgttgcatac
   122221 cgtcctgtaa ggaatttagt tgcttctcga tatgttttac tattagcttt taatgctcct
   122281 gcataaaaat ctttatttcc actaattccc ttagttaata atttggcata atcttcaaag
   122341 ctttcctcat agttttcata cactctaaat tttgattggg tcgtataaag cgttccatta
   122401 cccaaatctt cgtttgttac cataatgaca aagtttccat tataagttcc ttttatccca
   122461 aataaattgt aattcggtgc ctgagctaac tggctttgac cactggcaga ttctaagatc
   122521 gcttgtgcaa tcataacaga agcatataaa tcatatttct gaccaatttt tctcgcagac
   122581 tcaccaattt tcataataaa attttctaca gattcatttt tgtcaaaatg aatttcttca
   122641 ttaccagata gtattggttg tgattggaaa gattctgttg attctatttg ctgtggtgtc
   122701 gaaaaagttt ttgtttgtcc ttccgttttt ggcgcttcct tttttgaaga ttctatatgg
   122761 tttggttttg tcactttcga agaagttatt tcacttgtac tacttgaagt tgttgaagat
   122821 tcggtacttg aaatcgtact actttcactt gacgaagttg ttgaagactc ggcatttgaa
   122881 gtcgtactac tttcacttga tgaagttgtt gaagactcgg catttgaagt cgtactactt
   122941 tcacttgatg aagttgtcaa aagttcacta ctctctgctg ttacatcgga attactcatt
   123001 tcagtcgtct cggttctttc ttttgcaatt ttgtatgttg aattttcgct tgccaacaca
   123061 tggaaaggtg ttgctataag gagttgggac attaatataa ctgctgatgc aagttttaaa
   123121 gattttttca tttgatttac ctctcttttt tctctttaat agtgttttct attaaaaatt
   123181 atgataaata ttttcaatga attgagtaat acagtatatt tcttgatttc atttagtaac
   123241 gattatgttt caatgtttaa tcctgttact gcctttaaaa taaaattata gggttggcta
   123301 aaactaggat gaaataaata atctaacatt ttcaatttgt ctaatgtaat ttgttcttct
   123361 atagcaagag aaaacattga taatagtcca gataaatctt cttgactaca aagttgtaca
   123421 cctactattc tctttgtatt tttttcaaag ctaattctta attttacacg actatttttt
   123481 ctcaaaaaag caggtctaat gaaatcttct atttctatat aatctataga ctcacttaat
   123541 aatcgtgctg tttttaaatt caaacctgct gcgtaaaaat tgtaattaaa tatagaaaag
   123601 ccatttgtaa attgggaacc agaaggtgta aaatcttgat tgttaatatg ttgagttgca
   123661 atataagctg ttcttagagc attagaaata gaaaaatcaa cactacttct attaatggac
   123721 gtaaaaaaag ttgtagcaca atccccaata gcatatatat cttttatatt tgtttgctgg
   123781 tattcattag ttagatatgc tccactaaca tctctcaaga gttccttttc cccaattgga
   123841 ctattagata gaaatcctat agcttgtacg accatatcca tagagtattg ggtaccactt
   123901 gtagtgacta ttttttcaat cttttttctt cctaaatatt ttttaggact ttctgataga
   123961 caaagatgta ttccattttt tgatagtatt tcttcaactt tatctgaaaa actacggtcg
   124021 taataagtcg acaacactct atctgcaaca tcgaaaagat atacttcttt ccctcttttt
   124081 cttatggctt cagcaatttc aactccaata tatcctgctc caataattcc tactttttta
   124141 atagtactat catccaaagc tttaaaaatc tcttttgcat ttggaaaatt ctttatcgaa
   124201 aaaatattag ctaaattatt ccccggtata tctagcataa tttgctttga acctgtagct
   124261 aatataactt tgtcatattc ttgagagaat cttgtaccat ctttttttac acaactaatc
   124321 tttttatttt gaatatcaat tttgtcaact tctgtctcta gaaataactt ttttatctca
   124381 gaatttagtg tcattctatt ttgataaaaa agatcttcta aattacttac ttctcctttt
   124441 ataaataacg atgtcccaca ttccaagtaa ctaatttctg attttttatc gattagaata
   124501 acttcataat ttgaatcgct taagtttaga aatcgagctg cggctagccc tgaatggttt
   124561 gaaccaataa ttactatttt ttccacttaa ttacattctt ttcctttcta taaaaagtat
   124621 attcatcttt aactaacact tatttcccat cataaaaaag caaaaagagg aagaaaaaga
   124681 ttcttcctct tctcagagaa gacttaagaa attgattttc tattcattct ctttttttcg
   124741 atttttatac cctaataatc ctaacgttcc taacagtatt aaaccaccgc taatagtaaa
   124801 gatattactg tcactctctc ccgtcttagg aaaaactttt gaggactggc ttttgcctct
   124861 atttttgtct tgattgctgt tgctaccttg attgtcagtc ttactacctt tactaccgtt
   124921 ttttccactg tcttttttgt tggaagaaac aactacatta gtaggttcac tagtattacc
   124981 agattcatca gttgcagtta ctatcacttt tgtccctgcc tctaatttag gaactttaac
   125041 tgtaaaatta ccatttttat cagctgtccc atggtgttga cttccgtctg gtaatgtaac
   125101 tgtcacctct gcatttggtt cagttttacc agtaacaatt tggtcaccct ctgtaatagg
   125161 atcaacttga ggtttattcg gtgctttttt atctactaca gaaatagtaa cttctacttc
   125221 ttctttagat ccatctggat atgttactac tacagtacct tttttgtttc cagctgtact
   125281 aacatctact ttttctttcc aagtataagt agttcctttt ggtagatctt ctttgttctt
   125341 aattccctca gatgcatctg gctctttatt taagtctgta gtcacttttt gtccttcagg
   125401 attatattta tctgcatcgg aacgagtatc tgtcactgta acgtctactt caacttcatc
   125461 agatgatcca tctggatatg tcactacaat agtacctttt ttatttccag ctgtgctaac
   125521 atctactttc tctttccaag cataagtagt tccttttggt aaatcttctt tgttcttaat
   125581 tccctcagat gcatctggct ctttatttaa gtctgtagtc actttttgtc cttcaggatt
   125641 gtatttatct gcattagaac gattgtctgt cacttctact ggaactttca ccgtatcttt
   125701 cgtaccgtct ggatacgtta cttcaatgac accttcataa ttgcctggtg tgttggtatc
   125761 aattgtaccg ccgggagtaa catctgttac tgttgttcct tctggtaacg ctggcaagtt
   125821 agtaacatta tctgttaaat ctactgtgcc tccaatttct actttttcgc cttctactgt
   125881 tggttcatat ttatcggcgt cagaacgatt gtctgtcact tctactggaa ctttcaccgt
   125941 atctttcgta ccgtctggat acgttacttc aatgacacct tcgtaattac ctggtgtgtt
   126001 ggtatcaatt gtaccgccgg gagtaacatc tgttactgtt gttccttctg gtaacgctgg
   126061 caagttagta acattatctg ttagatctac tgtgccccca acttctactt tttcgccttc
   126121 tactgttggt t
//
LOCUS       DAYYBH010000002       115907 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000002 DAYYBH010000000
VERSION     DAYYBH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 115907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 115907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..115907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.002"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(606..962)
                     /locus_tag="ACHAUG_000119"
     CDS             complement(606..962)
                     /locus_tag="ACHAUG_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8479246.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="ACHAUG_000120"
     CDS             complement(976..1386)
                     /locus_tag="ACHAUG_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8479247.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="ACHAUG_000121"
     CDS             complement(1732..3918)
                     /locus_tag="ACHAUG_000121"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8479248.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="ACHAUG_000122"
     CDS             4136..4819
                     /locus_tag="ACHAUG_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8479249.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="ACHAUG_000123"
     CDS             complement(4900..5619)
                     /locus_tag="ACHAUG_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8479250.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="ACHAUG_000124"
     CDS             complement(5931..6137)
                     /locus_tag="ACHAUG_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479251.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="ACHAUG_000125"
     CDS             6382..6984
                     /locus_tag="ACHAUG_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8479252.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8405
                     /locus_tag="ACHAUG_000126"
     CDS             7125..8405
                     /locus_tag="ACHAUG_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8479253.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8418..9146
                     /locus_tag="ACHAUG_000127"
     CDS             8418..9146
                     /locus_tag="ACHAUG_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8479254.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9215..10738)
                     /locus_tag="ACHAUG_000128"
     CDS             complement(9215..10738)
                     /locus_tag="ACHAUG_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8479255.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10990..12171
                     /locus_tag="ACHAUG_000129"
     CDS             10990..12171
                     /locus_tag="ACHAUG_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8479256.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12231..13859)
                     /gene="treC"
                     /locus_tag="ACHAUG_000130"
     CDS             complement(12231..13859)
                     /gene="treC"
                     /locus_tag="ACHAUG_000130"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8479257.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14310..17021)
                     /locus_tag="ACHAUG_000131"
     CDS             complement(14310..17021)
                     /locus_tag="ACHAUG_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8479258.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17031..17732)
                     /locus_tag="ACHAUG_000132"
     CDS             complement(17031..17732)
                     /locus_tag="ACHAUG_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8479259.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17918..18517
                     /locus_tag="ACHAUG_000133"
     CDS             17918..18517
                     /locus_tag="ACHAUG_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8479260.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18598..19611
                     /locus_tag="ACHAUG_000134"
     CDS             18598..19611
                     /locus_tag="ACHAUG_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8479261.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19719..20636
                     /gene="rbsK"
                     /locus_tag="ACHAUG_000135"
     CDS             19719..20636
                     /gene="rbsK"
                     /locus_tag="ACHAUG_000135"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8479262.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20664..21059
                     /gene="rbsD"
                     /locus_tag="ACHAUG_000136"
     CDS             20664..21059
                     /gene="rbsD"
                     /locus_tag="ACHAUG_000136"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8479263.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21059..22432
                     /locus_tag="ACHAUG_000137"
     CDS             21059..22432
                     /locus_tag="ACHAUG_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8479264.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22789..24933
                     /locus_tag="ACHAUG_000138"
     CDS             22789..24933
                     /locus_tag="ACHAUG_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8479265.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24985..26025)
                     /locus_tag="ACHAUG_000139"
     CDS             complement(24985..26025)
                     /locus_tag="ACHAUG_000139"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8479266.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26302..28104)
                     /locus_tag="ACHAUG_000140"
     CDS             complement(26302..28104)
                     /locus_tag="ACHAUG_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8479267.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28118..28870)
                     /locus_tag="ACHAUG_000141"
     CDS             complement(28118..28870)
                     /locus_tag="ACHAUG_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8479268.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28879..29241)
                     /locus_tag="ACHAUG_000142"
     CDS             complement(28879..29241)
                     /locus_tag="ACHAUG_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8479269.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29417..30289)
                     /locus_tag="ACHAUG_000143"
     CDS             complement(29417..30289)
                     /locus_tag="ACHAUG_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8479270.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30340..30828)
                     /locus_tag="ACHAUG_000144"
     CDS             complement(30340..30828)
                     /locus_tag="ACHAUG_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8479271.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30966..31757)
                     /locus_tag="ACHAUG_000145"
     CDS             complement(30966..31757)
                     /locus_tag="ACHAUG_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8479272.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31812..32420)
                     /locus_tag="ACHAUG_000146"
     CDS             complement(31812..32420)
                     /locus_tag="ACHAUG_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8479273.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32413..32730)
                     /locus_tag="ACHAUG_000147"
     CDS             complement(32413..32730)
                     /locus_tag="ACHAUG_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8479274.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32886..33614)
                     /locus_tag="ACHAUG_000148"
     CDS             complement(32886..33614)
                     /locus_tag="ACHAUG_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8479275.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33773..34654
                     /locus_tag="ACHAUG_000149"
     CDS             33773..34654
                     /locus_tag="ACHAUG_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8479276.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34891..35934)
                     /locus_tag="ACHAUG_000150"
     CDS             complement(34891..35934)
                     /locus_tag="ACHAUG_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8479277.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35937..36605)
                     /locus_tag="ACHAUG_000151"
     CDS             complement(35937..36605)
                     /locus_tag="ACHAUG_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8479278.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36747..39083
                     /locus_tag="ACHAUG_000152"
     CDS             36747..39083
                     /locus_tag="ACHAUG_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8479279.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39198..39509)
                     /locus_tag="ACHAUG_000153"
     CDS             complement(39198..39509)
                     /locus_tag="ACHAUG_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479280.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39690..41165
                     /locus_tag="ACHAUG_000154"
     CDS             39690..41165
                     /locus_tag="ACHAUG_000154"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8479281.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41212..41796
                     /locus_tag="ACHAUG_000155"
     CDS             41212..41796
                     /locus_tag="ACHAUG_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8479282.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41798..42217
                     /locus_tag="ACHAUG_000156"
     CDS             41798..42217
                     /locus_tag="ACHAUG_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8479283.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42498..42782)
                     /locus_tag="ACHAUG_000157"
     CDS             complement(42498..42782)
                     /locus_tag="ACHAUG_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8479284.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43134..43844)
                     /locus_tag="ACHAUG_000158"
     CDS             complement(43134..43844)
                     /locus_tag="ACHAUG_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8479285.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43841..45313)
                     /locus_tag="ACHAUG_000159"
     CDS             complement(43841..45313)
                     /locus_tag="ACHAUG_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8479286.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45451..45924
                     /locus_tag="ACHAUG_000160"
     CDS             45451..45924
                     /locus_tag="ACHAUG_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8479287.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(46077..46628)
                     /locus_tag="ACHAUG_000161"
     CDS             complement(46077..46628)
                     /locus_tag="ACHAUG_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8479288.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46733..47263
                     /locus_tag="ACHAUG_000162"
     CDS             46733..47263
                     /locus_tag="ACHAUG_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8479289.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47746..48189)
                     /locus_tag="ACHAUG_000163"
     CDS             complement(47746..48189)
                     /locus_tag="ACHAUG_000163"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8479290.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48205..49365)
                     /locus_tag="ACHAUG_000164"
     CDS             complement(48205..49365)
                     /locus_tag="ACHAUG_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8479291.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49872..51572
                     /locus_tag="ACHAUG_000165"
     CDS             49872..51572
                     /locus_tag="ACHAUG_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479292.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51674..52507)
                     /locus_tag="ACHAUG_000166"
     CDS             complement(51674..52507)
                     /locus_tag="ACHAUG_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8479293.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52811..54976)
                     /locus_tag="ACHAUG_000167"
     CDS             complement(52811..54976)
                     /locus_tag="ACHAUG_000167"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8479294.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55236..57530
                     /locus_tag="ACHAUG_000168"
     CDS             55236..57530
                     /locus_tag="ACHAUG_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8479295.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57523..58188
                     /gene="pgmB"
                     /locus_tag="ACHAUG_000169"
     CDS             57523..58188
                     /gene="pgmB"
                     /locus_tag="ACHAUG_000169"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8479296.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58181..59188
                     /locus_tag="ACHAUG_000170"
     CDS             58181..59188
                     /locus_tag="ACHAUG_000170"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8479297.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59210..60229
                     /locus_tag="ACHAUG_000171"
     CDS             59210..60229
                     /locus_tag="ACHAUG_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8479298.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="ACHAUG_000172"
     CDS             complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="ACHAUG_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8479299.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61986..63407)
                     /gene="tyrP"
                     /locus_tag="ACHAUG_000173"
     CDS             complement(61986..63407)
                     /gene="tyrP"
                     /locus_tag="ACHAUG_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8479300.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="ACHAUG_000174"
     CDS             complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="ACHAUG_000174"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8479301.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65818..67074)
                     /gene="tyrS"
                     /locus_tag="ACHAUG_000175"
     CDS             complement(65818..67074)
                     /gene="tyrS"
                     /locus_tag="ACHAUG_000175"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8479302.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(67186..67391)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            67654..68955
                     /locus_tag="ACHAUG_000176"
     CDS             67654..68955
                     /locus_tag="ACHAUG_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8479303.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(69024..69623)
                     /locus_tag="ACHAUG_000177"
     CDS             complement(69024..69623)
                     /locus_tag="ACHAUG_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8479304.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            69877..70758
                     /locus_tag="ACHAUG_000178"
     CDS             69877..70758
                     /locus_tag="ACHAUG_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8479305.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            70771..72381
                     /locus_tag="ACHAUG_000179"
     CDS             70771..72381
                     /locus_tag="ACHAUG_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8479306.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(72458..73117)
                     /locus_tag="ACHAUG_000180"
     CDS             complement(72458..73117)
                     /locus_tag="ACHAUG_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8479307.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(73400..74065)
                     /locus_tag="ACHAUG_000181"
     CDS             complement(73400..74065)
                     /locus_tag="ACHAUG_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8479308.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(74212..74817)
                     /gene="dhaL"
                     /locus_tag="ACHAUG_000182"
     CDS             complement(74212..74817)
                     /gene="dhaL"
                     /locus_tag="ACHAUG_000182"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8479309.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(74845..75855)
                     /gene="dhaK"
                     /locus_tag="ACHAUG_000183"
     CDS             complement(74845..75855)
                     /gene="dhaK"
                     /locus_tag="ACHAUG_000183"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8479310.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(75830..76225)
                     /gene="dhaM"
                     /locus_tag="ACHAUG_000184"
     CDS             complement(75830..76225)
                     /gene="dhaM"
                     /locus_tag="ACHAUG_000184"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8479311.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            76379..76699
                     /locus_tag="ACHAUG_000185"
     CDS             76379..76699
                     /locus_tag="ACHAUG_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8479312.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(76811..77668)
                     /locus_tag="ACHAUG_000186"
     CDS             complement(76811..77668)
                     /locus_tag="ACHAUG_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8479313.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(77776..79080)
                     /locus_tag="ACHAUG_000187"
     CDS             complement(77776..79080)
                     /locus_tag="ACHAUG_000187"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8479314.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(79077..79586)
                     /locus_tag="ACHAUG_000188"
     CDS             complement(79077..79586)
                     /locus_tag="ACHAUG_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479315.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(79761..81896)
                     /locus_tag="ACHAUG_000189"
     CDS             complement(79761..81896)
                     /locus_tag="ACHAUG_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8479316.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(81979..83634)
                     /locus_tag="ACHAUG_000190"
     CDS             complement(81979..83634)
                     /locus_tag="ACHAUG_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8479317.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(83636..83950)
                     /locus_tag="ACHAUG_000191"
     CDS             complement(83636..83950)
                     /locus_tag="ACHAUG_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8479318.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            84120..84380
                     /locus_tag="ACHAUG_000192"
     CDS             84120..84380
                     /locus_tag="ACHAUG_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8479319.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            84394..84708
                     /locus_tag="ACHAUG_000193"
     CDS             84394..84708
                     /locus_tag="ACHAUG_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8479320.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(85438..85992)
                     /locus_tag="ACHAUG_000194"
     CDS             complement(85438..85992)
                     /locus_tag="ACHAUG_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8479321.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(86118..88047)
                     /locus_tag="ACHAUG_000195"
                     /pseudo
     CDS             complement(86118..88047)
                     /locus_tag="ACHAUG_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(88353..89069)
                     /locus_tag="ACHAUG_000196"
     CDS             complement(88353..89069)
                     /locus_tag="ACHAUG_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8479322.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(89070..90893)
                     /gene="glpO"
                     /locus_tag="ACHAUG_000197"
     CDS             complement(89070..90893)
                     /gene="glpO"
                     /locus_tag="ACHAUG_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8479323.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(90907..92403)
                     /gene="glpK"
                     /locus_tag="ACHAUG_000198"
     CDS             complement(90907..92403)
                     /gene="glpK"
                     /locus_tag="ACHAUG_000198"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8479324.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(92539..93999)
                     /locus_tag="ACHAUG_000199"
     CDS             complement(92539..93999)
                     /locus_tag="ACHAUG_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8479325.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(93989..95284)
                     /locus_tag="ACHAUG_000200"
     CDS             complement(93989..95284)
                     /locus_tag="ACHAUG_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8479326.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(95616..96293)
                     /locus_tag="ACHAUG_000201"
     CDS             complement(95616..96293)
                     /locus_tag="ACHAUG_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8479327.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(96323..97414)
                     /locus_tag="ACHAUG_000202"
     CDS             complement(96323..97414)
                     /locus_tag="ACHAUG_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8479328.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(97515..97949)
                     /locus_tag="ACHAUG_000203"
     CDS             complement(97515..97949)
                     /locus_tag="ACHAUG_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8479329.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(98533..99990)
                     /locus_tag="ACHAUG_000204"
     CDS             complement(98533..99990)
                     /locus_tag="ACHAUG_000204"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8479330.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(99987..101882)
                     /locus_tag="ACHAUG_000205"
     CDS             complement(99987..101882)
                     /locus_tag="ACHAUG_000205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8479331.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(101940..103715)
                     /locus_tag="ACHAUG_000206"
     CDS             complement(101940..103715)
                     /locus_tag="ACHAUG_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8479332.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(103765..105261)
                     /locus_tag="ACHAUG_000207"
     CDS             complement(103765..105261)
                     /locus_tag="ACHAUG_000207"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8479333.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(105455..107077)
                     /locus_tag="ACHAUG_000208"
     CDS             complement(105455..107077)
                     /locus_tag="ACHAUG_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8479334.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(107405..108718)
                     /locus_tag="ACHAUG_000209"
     CDS             complement(107405..108718)
                     /locus_tag="ACHAUG_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8479335.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(108963..109187)
                     /locus_tag="ACHAUG_000210"
     CDS             complement(108963..109187)
                     /locus_tag="ACHAUG_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479336.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(109207..109629)
                     /locus_tag="ACHAUG_000211"
     CDS             complement(109207..109629)
                     /locus_tag="ACHAUG_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8479337.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(109771..110481)
                     /locus_tag="ACHAUG_000212"
     CDS             complement(109771..110481)
                     /locus_tag="ACHAUG_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8479338.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(110499..110996)
                     /locus_tag="ACHAUG_000213"
     CDS             complement(110499..110996)
                     /locus_tag="ACHAUG_000213"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8479339.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(111033..111485)
                     /locus_tag="ACHAUG_000214"
     CDS             complement(111033..111485)
                     /locus_tag="ACHAUG_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8479340.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(111620..112225)
                     /locus_tag="ACHAUG_000215"
     CDS             complement(111620..112225)
                     /locus_tag="ACHAUG_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8479341.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(112675..113295)
                     /locus_tag="ACHAUG_000216"
     CDS             complement(112675..113295)
                     /locus_tag="ACHAUG_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8479342.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(113297..114166)
                     /locus_tag="ACHAUG_000217"
     CDS             complement(113297..114166)
                     /locus_tag="ACHAUG_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8479343.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(114189..114596)
                     /locus_tag="ACHAUG_000218"
     CDS             complement(114189..114596)
                     /locus_tag="ACHAUG_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8479344.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(114615..115904)
                     /locus_tag="ACHAUG_000219"
     CDS             complement(114615..115904)
                     /locus_tag="ACHAUG_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8479345.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        33904 a        23946 c        20250 g        37807 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7381 ttttaggaga tcacttaaca agcatcggcg atttttttgt tttgaaatct tcggttattg
     7441 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7501 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7561 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7621 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7681 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7741 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7801 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7861 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7921 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7981 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     8041 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     8101 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     8161 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     8221 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     8281 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8341 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8401 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8461 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8521 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8581 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8641 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8701 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8761 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8821 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8881 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8941 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     9001 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     9061 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     9121 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     9181 agccgatcca aacccttcgg ttcttttttg cctatcagtg caaaagcatt tcctgtgcaa
     9241 tttcacgtcc atccattgta tctggataaa atgttggcca attctccatt tctttcagca
     9301 atacttcttg actttcattt cctgcatata aatgaaaatg ttcggagtca gccggtgcaa
     9361 tcgtgtgatc actgaattga atatatctaa aagcgccact ttccttatct tctgcttcga
     9421 atagatacct tacgccacgg tttcctgcct cgtaggtcaa gatttctttc cctacataac
     9481 gataatttga acgtttcgtt gttccattct taaacgtaaa tgccatctct ttctcactga
     9541 tcttgattga agcaatatct gtttgatagc ccttcgtata atattcttta tattcttgtg
     9601 ctgtcttatc ctgattcaat ttttctttat attcaaaaac aggatcaaaa gtcccatctt
     9661 ctacatacgt atctactgtt tgccaagttc ctgcccaatc tgaaagagag cgatcttgta
     9721 catcctcgtc ttcaaaataa ccgttgtaga cgctcttttc ttttttgggg gtcaaagtat
     9781 cttctagcgg gttttctgtc tctgtcgttt ttttcaacgc ttcgaggttt gcttccatga
     9841 tagaaagata gttttctcca ttttctattt gttcattcgt caatccttcc aaaggattca
     9901 atacttctaa agatacacct gcttcgtttg ctaacgttct ggcaatacta tctttggcgt
     9961 tttcttcaaa ataaatatag ttgattccat gttctttaac aaagtgtttc agttcagcaa
    10021 ttctcgccgc agacggttct tcactagaag aaagaccagc aatcgatact tgattcaatc
    10081 catattctaa cgctagatag ttgaatgcag catgttgcgt gacaaaagat ctttgttttg
    10141 cttgcttaaa ggtttcttga taaagctgat ggagtgcttg aagtttctcg gtataggctg
    10201 ctgcattttt tgtccatact tcctgttttt ctgggtaagc ttcaatcagc tgatcacgta
    10261 tgtttgctac ctgcttgatt gctaaacttg gagctagcca tacatgtggg tctagctcat
    10321 gtgtatggcc ttcttccccg tgttcatggt catgatcatg tccttcttca cttcccggaa
    10381 gtaaaagcat accttctgtc gcttttatcg tatggacatt gccttcttgt aatgtttttt
    10441 ggattgcggg gacccatgtt tccatgtttt catcattata aacaaagata tctgtatctt
    10501 ggatctttgc catgtcttta gcagaaggtt cataatcgtg ggaatcagtc cctgctggaa
    10561 tcaataactc tacttctcct tcatccccga cgattgcttt tgtaaaatca tacatgggat
    10621 aaaaggtcgt catcacgctc aatttttctt tactgtcctc ggctttccca cttgccccgc
    10681 aagcagttaa catacctgct aaaaccagca tggccaatat acctatcaat cgtttcattt
    10741 atgacactcc tcttctcatc ttctttcatt tgaatgttcc gattcgcttt tcgcaaaccg
    10801 taatagttac gttttagcaa caggatttaa tttatcatat ggttaaaaga actgtcaagc
    10861 gttttcttat tttaattgat gagaaagttt atatgaatga ttgacgatat ttttgcaatc
    10921 aacctctatc attttccttt tatacccgct tttgtctata atgaatttat agataaagga
    10981 ggaaaaagag tgaaaaaaga aacagataca acgaatcgtt tgatccgctt tttaggagga
    11041 aaaacctctt actatgtttt aggaatagtt atcttacttg cgctgatcat ttattttttc
    11101 catcagatct cgtttatatt caagcctgtc ggaatcatta tcgctactat cttgccccca
    11161 ttattgtttg cactgatctt gtattatctt ttggaaccag tcatcaaacg tttgagcaag
    11221 cacctttcga ggaacttgtc agtcattatc gtttatgtga ttattttatt tttattgggg
    11281 cttggaagca tatggcttat tccttttctt gaagaacagg caaagacatt gatcagtagt
    11341 ttgccagatt tgtttcaaga ttttcaagag agtctccgac aatttttgga gaaaacacct
    11401 tttgccgatt catttaatca atttttcgct tctattgacg acgtgacgtc tgatatagct
    11461 ggatttattg gaaattattg ggaaagtggc gcacaacgga tcagcaacat tttttcaact
    11521 gttacagcta tttttattac tttatttact ggtccaattg tcgctttttt ccttttgaga
    11581 gatcctcaaa aattttatca gtcagtttta gcgattgtcc caccagcatt tcgtacagat
    11641 tttaaaaatt tgacaaagat tgctaatcaa caattgggtt catttctaaa ggggcaaatc
    11701 attgcttcat ttatccttgg agcagtctac tgggtttgtt tcttactgat cggattagaa
    11761 tttgctagtg ttttagctat ttctgctggg ttgttatgta tcattccata tattgggccg
    11821 tttatcgcct tctttccagg tttgatcatt gcattccaag actctacttt tatggctgtc
    11881 aaatttgtca ttgtctggtt tgccgtacaa cttctccacg gtgacttggt gattcctcga
    11941 gtaatgggtg atcgtttaca gatccatccg attacgatct taatcgtttt acttgtcatg
    12001 ggtgatctaa tgggaattgt gggcgttatt tttggtattc ctatctatac gttagtcaaa
    12061 ttactcgtta cttttatttt ccgtaaattc aaacaacgct acaataaatt ttatggcgat
    12121 aaaggagagt atgaaaactc ttcattttct gaagaagatt attttaaata aaacacaaaa
    12181 cgtcgatctt acgagaatag ctcgtaggaa cgacgttttg tgtttttgtt ttaatttttc
    12241 agcaaagcaa ttgcctggta tggttttagc tccagacttt gatgaagaac agtatcggaa
    12301 taattatcga taagaatatt ggcttctgtg aatccttctg ggatggcgat ccttgtatct
    12361 ttatcataga aatttgttaa tacaagcagc tgctgtcctt caaattggcg aagaaagcca
    12421 taaacttccg gatgatttgc ttcataagca tgataatctc cttgtgcaac gattggatat
    12481 gtttttctca gccgaatcaa tttcttgtaa taggacaaaa tagaatcttc agaagatagt
    12541 tctttttcta cattaatctc ttctagcttg cctccaactt ttaaccaagg tttccctgta
    12601 gtaaatcctg cttgttctgt gtctgaccat tgcatgggtg ttcgagagtt atcgcgagat
    12661 tttgctttga tccgacgaaa cgcttctttt tctgtgaatc cttgttcaag gagtaactta
    12721 taagcattca aactttctac gtcaatatag tcggaaatcg atgaatagtc tgggtcgatc
    12781 atgccgattt cttctcccat ataaatatag ggcgtcccac ggttcaaatg gatcatggtt
    12841 gctagcatct ctgctccttg ttttcgatac tgatcgatag ctacaaatcg attgagcgca
    12901 cgtggctgat catgattatt aaaaaataga gcattccagc cgttaccctc actcatcttt
    12961 tctccccatg tatgaaatag tcgctttaat tctgcaaaat caaatggagg agtagaccat
    13021 ttattcccat tttcataatc gactttcaaa tgatggaaat taaagaccat tgataattct
    13081 tctctactag gttctgtata caagatacaa ttttcgatcg tcgtagagct catttcaccg
    13141 actgtgatga tagtggaatc cgtcccaaac gttgcttggt tcaattcttt tagatacgta
    13201 tgggtgattg gtttgtccgt atattcaggt ttgccatcat tttctttatt atttttcaat
    13261 tgttcgtctt taccgataac attgataaca tcgaaacgga atccttttac ccctttttcc
    13321 atccaaaaac gaacaacttt gaatagttcc tctcttactg cttggttccg ccagttcaag
    13381 tcagcttgtg tccgatcata taagtgcaaa taatatttcc ccgtatctcc aaatggcgac
    13441 caagcacttc caccaaattt tgatacccaa tccgttggct cttctcttaa aatgaagaag
    13501 tcctgataat gctggtcccc tgctagtgct tttttgaacc attcatgttc tgtcgaaacg
    13561 tgattcagca ccatatcgag catgatttct atccggtatt tctttgcttc ggctattagc
    13621 tgttcgaatt cctccatcgt accaaagata ggatcgatgg ctgtgtaatc agagatatca
    13681 taaccgttgt ctttttgagg gcttggataa atagggttta gccaaatcat gtcgatgcct
    13741 agatcattca gatacgggag tttttctttg attcctttta aatctccaat tccatcacca
    13801 ttactgtctt tgtaagattt gggatagatt tggtaaatga ctttgtcttt aaatgtcatg
    13861 gatatttacc tccattcaac tgatttatta cctaccacaa cttatatata caagttatca
    13921 tttattatta taactgaacc gaaagcgttt gtaaaattta tctttataca ttctaagagg
    13981 tctgttgccc aataaaaaac aagagatcag acatgtattt gtctatcctc ttgtttaaaa
    14041 tagcaaaata ttcttgtttc ttctataaat ggtgttcaaa agcttttatc tacggcaatc
    14101 agtcttctca aatgtaaaac atgaggagct aattttattt gagtattctt tttgctcgat
    14161 gcaggacggc ttttttacaa cctctgattc atggtgttca atcagctgtc cttcggtatt
    14221 agctcaaaaa acggcaaaat atgaaaaact attttgccgt ttgtttctct aatactcagg
    14281 tcaaaccgct gatttcacaa cctctgtttt tattcattcg atttcaacgc atcaatcata
    14341 tcgatatgct ttaatttgaa atgggtaaca atcatcacaa taaccgtaaa tacgatagtt
    14401 aatcctgctg ataagacata agcccaccat gtgatcacta atggaaagat gacattctcc
    14461 atagacgctt gttgcaagat gaagtcagtc aatatatatc ctacaccaaa tcctccgatg
    14521 attcctaaaa gagtaaagat gatattttct cgaacaatgt acatcgtgac ttctttatca
    14581 aaaaatccaa ggacttttat tgtagataat tcgcgtacac gctcggaaat attgatatta
    14641 gttagattat acaagacgat aaaagccagc agtcctgata atacgacaaa gattaagaca
    14701 accgagtcta agttcgccat tgattcttct tgcgtttcga tttgtttgga catgaaagac
    14761 gtatttgtca cagcgtctgt ggcaagcagt ttctcggaaa gcatttcttc ttcttttgtt
    14821 gtcatcttat ctgtacgcag caaaaatgca ttatccatca tcgattgatc agtaactgaa
    14881 gcataatatt caggggacat ataaataaag ctacctagat agtttttcac gatttctttt
    14941 acttcgactt tccattcttt ttgatcttca tcataaagcg aaatatgatc tcctgtagat
    15001 aagtcaaata gttctgctac tttttgcgta atagcgattc ctgttttagg caaggagcgt
    15061 gggttctctt caagatcagt taaatggata aaacgatcca aatcagctgt tttacttggt
    15121 acgatcactg tcagactttg cgaagcatgt cctttttttc tcaattctac ggtttgagta
    15181 tgaatagcta ggatctcatt tactttttct tgcttctgca gcatatccgc tgctttttga
    15241 cgactatctt ccgtggatga atccaatgta acgattgctt gatagtcaat gatcggtcca
    15301 aattgtttgg ctgaaacaga acttagagaa tctttcaatc ccacgcctgc aaccatcagc
    15361 ccggcacatc cagcaattcc gataatcgcc ataagcattc ttgacttata tcggaagaga
    15421 tttcggtaac tgattttttg attaaagcta aggcgtgacc acagcggagt gatgtattca
    15481 agaaaaatgc gtttccctgg cttcggtgct ttcggttgaa gcaatgcagc aggccgttct
    15541 cttaaatctt tgaataaaac gatcattgca gcgccaaatg cggcaatgaa gaatgcggca
    15601 gctgcttgta aaatcggcgg ccagtcataa aaaataagcg cactcccaat atcataccgc
    15661 tcgttagaca gctcaaaaat gatccttggc aaaagattcg ttcctaatat cgttcctaaa
    15721 acaataccta tccctgaggc tagacttgca tagattgcat atttttttgc gatttcaact
    15781 ttcgtataac ctaacgcctt taatgtcccg atttccctgc ggttttcttc taccatacgt
    15841 gtcattgtcg taaaggtgat caatgcggca ataaagaaaa agaaaacagg aaaaacattg
    15901 gcaatcgcag cgatacgatc agacagatca ccaaattctt gaaaaccgac attttctgaa
    15961 cgttcattgt ataggtatga tggtttagtt aaattttcaa ttttttctgc atttcgattc
    16021 aattcttcct ctgcagaggc cagttcacgt tctttctcaa ctaattgcct ttcttgttca
    16081 gcaagttgag cttcttgttg agataatgtt tgaacagcga ttgttcgttg cgcttcagga
    16141 agctgctgga tagttgctga ctgttgcgct aactgttttt tagcatcttc taaccgtgtt
    16201 cttgcttgcg ttgtttgtgc tttgccatcc gctatttttt gctgtgccgg ttctaattct
    16261 tcgttggccg attttttcag ctcttctaag cgttcttcgg gtcgatcttg taaatacttt
    16321 tctgcctttt cttgattttg ctccatttta tctttgtaag ctttgctgta cgtattcaaa
    16381 ttctttacgt ttgtaaaatc aagatataag acagaataaa tatctgtttt aaaaacggat
    16441 tcaggtaaat agacaaaata atcgacgcta ccatttccga cgttcgcgta tcctctctcc
    16501 aaattgttga tgaacaatgg agaacggaca aagcccgtga tggtatactc tttttctttc
    16561 aattcatctg aatcaatctg ataaatatca ccgattttat acccttcatc ttccgctttt
    16621 tcatccaata caagctggtt tggtttttcc gggagttttc catcagttaa ttgtagcttg
    16681 ttctgcttgt tatttttgtt ataagaaaat atttgaatca cttcgttttt gccagtattg
    16741 gaataataga tctgttttcc tgtttctact tttgcatctg ggatcttttc tgcttgagca
    16801 atatcttttt gtgtcaagcc accagtagaa acgatttgca aatcagaaag tcctgctgta
    16861 ccaacatagt gatccatcgt tttttgcatg acaggtccag cagttttgac acctacaaat
    16921 aaaagtgttc ctagcatgac aataaggatg attgcaatga atcgtccttt agattgccga
    16981 atttcgcgaa aacctgcttt aagatatgtt cttgttttca taaaatcccc ctaccataca
    17041 atctcatcaa tggatgaagg atgatcattt aattcaacag accttacttt agcatcatta
    17101 atgtgaatga ctctatcagc gatgggcgct aaagcagaat tatgtgtaat gatcaaaacg
    17161 gtattgccgt gttgtctgct agcgttttgc aataatttca atacttgctt gcccgtttca
    17221 aaatccagcg cgcctgtcgg ttcatcacat aaaagaagtt tgggattttt agctagtgca
    17281 cgagcaattg aaaccctctg ctgttcgcct cctgataatt gggacggaaa gttattcaat
    17341 cgatgagcta ggcccacttg cctcaatacc tctacaggat caagcgcatc aggagaaact
    17401 tccgttgcca attccacatt ttcttttgct gttagattag gaacgagatt gtagaattgg
    17461 aaaacgaacc ctacatctgt tcgtcggtaa gcagtaagct gtttatctga aaattgcgca
    17521 atatctgtgt catcaataat gatctgacct tcatcaggtg tatccatccc acctaaaata
    17581 ttaagaatcg ttgactttcc tgctccactt ggaccaagta taatcactaa ttcgcctttt
    17641 tcaattgaaa aagaaatatc gtcatttgct gtaatggtcg tttctcccat tttgtatcgt
    17701 ttgtattcat tttttacttc gacatacttc actctgattc ctccattttc acgatatttc
    17761 tcgctatatc tatgcatcga atgcgttttc tcacacttat ttattttacc actgaatttc
    17821 tctagactaa agaaaggaat cctttaattt agttgacaaa taaacgattt atgatttact
    17881 tacataaaaa acattgttat ttgaaagaag gaacatcatg aatgatttaa aggaaaatat
    17941 gatttttgat ttatcaaaaa ctgcttttgt cgcgatcgac atgcaaaaag agatcgtaaa
    18001 taacggtaca ctctctccac acactgcagc atctattctg acaaacaatg atttgttagt
    18061 aagaacattg aaacgtaccg ctgccttgaa agttttagtg aacgtagaca tctccacttt
    18121 cccttatttg agtcaacaaa ctgatatggg tggaatgact gcttctgtac ctccagaatt
    18181 cactgattta cttttgaagg attctttgaa agatacagat aatatgttga ctatcacaaa
    18241 atacaatcca agtgcttttt ttggtacttc tcttgatctt caacttcgcc ggcgtggaat
    18301 cgaaactatc attttaagcg gtgtagcgac aacaaacggt gtatatgcta ctgcattaga
    18361 tgcctttcaa cacgggtacc atatcgtgct tgctgaagat gcttgcagtg atcgtgacaa
    18421 agaatcccat caattgttca taaaaaagat atttccgaaa actgcacgag tacgctcaac
    18481 gaaacaaata atcgaagcta tccaacaaag caaataagta ctggcacacg tgattttcat
    18541 ttacttcttt ttgatttcat gggaaaataa ccacaggatt tagataggag tatgaacatg
    18601 gtgaaaaaaa agatcacaat caaagaagtc gctaaacttt ccggcgtttc aatcacaact
    18661 gtctccttga tattaaatgg aaatacggag aaattcagtc cgaaaactgt tcagaaagtt
    18721 ttagctgcca aagaagaatt aggctatcag cctgactatt ttgctagaca aatgattacc
    18781 aaggaaacga aaacaattgg tgtactcgtt cctgatatca cgaatccatt cttcagtaca
    18841 ctgatgcgag gaattgagga tatcttgtat aagcagaatt tcgtcacgat attatgtaat
    18901 gctgattctg atcatcaaaa agaaatcgaa tatctagccg agctcacacg tcgaggtgtg
    18961 gatggattca ttatcgcgac ttctgctgtc tctacagatg caatcaacga aaatttgaaa
    19021 aaacaaggcc ggcccttcat tgttttggat cagaaaaaat ctgaaggatt tagtgatgcc
    19081 gtacgaacag acgattttcg cggtggttac ctagcgggca tgcacttgct tagtctagga
    19141 catcaaacga tcgcccttgt ctatcctgaa aatccaccgg aaaatgtcca cgctcggatt
    19201 gaggggttta aatctgctct tgatgtttac caaatccctc acgaccaact gatcctctta
    19261 cctacacagt tctcaaaaca aggtggctac cagatcacag cagaacttct tgattctgct
    19321 gcaactggtg tgtttgcttt aaatgatgaa ttagcttttg gattatatcg cggattagag
    19381 gaagctggaa aaagcatccc tgaagactac agcattattg gctatgataa tatagatatg
    19441 tgcgagtata tcaagccgaa actgacaacg atcgcacaac caatcttcga attgggacaa
    19501 acatctgcca agttattgtt agatcggatc caattcccag aaaaagaatg ggaagaaaaa
    19561 agattacctg tacgattaga aaaaagattt tcaactgcac ccttgaaata gctttcaagg
    19621 tgtgctatat ttttatcgaa ctattaaaac gttttactaa aacgttttaa aaaataattt
    19681 tcccatcaaa ataaatgaga tagttggagg tttgtaaaat gaacacggtt acagtaattg
    19741 gaagtattaa tatggacact acccttcgtt taaccaacat gcccaagcca ggcgaaacga
    19801 tgcatgcgca tgagattttt cacgctggcg gaggtaaagg tgccaaccaa gcagtggcag
    19861 ccaaacgttc aggcgcacgg acatctttta ttggaggcgt tggggctgac agcgaaggac
    19921 aacagttatt agatctactg acaaaagaaa acatcgatac atctgggata gctgaaatcc
    19981 aaggcgccac aaccggacaa gcaatgatta tggtagacgc agcaggagaa aatagtatct
    20041 tgatccatgc aggggcaaat aatgctttcc atgaacaaga agtcctaaaa aacaagcaac
    20101 tgattacaaa cagtgacttt attattgctc agtttgaaag tagcttagat gcgacgatcc
    20161 tagctttttc tattgcaaaa gacgcaggaa aaacaacgat cctgaatcct gcaccagctc
    20221 gtgaaacgat tcctactgaa ctacttgaaa agacagatat cattatacca aatgaaacag
    20281 aaacagaaat cattacagga atcagagtga cagatcataa cagtttggtc gcagcagccg
    20341 aaaaactcca tgaattaggt attggaacag taatcattac actaggcagt gccggcgctt
    20401 tctatcatac agaaaaagaa cacggtatcg tccctgcttt taaagtggat gccgttgaca
    20461 caacagccgc aggtgataca tttattggcg cattaagcag cacacttcag ccagacctta
    20521 gtaacttgaa agaagcgatc ctttatggaa atctagcctc ttctgttgct gtgcagtctt
    20581 atggtgcgca accttctatc ccttaccgcg aagctttaga acaatcagca gtctaaaatt
    20641 aacaatgaga ggaagaaaca aacatgaaaa aaggaaaagt aatcaattca gatatttcac
    20701 gagtaatcgc acaaatgggg cactttgata aattaagtat cggggatgca ggcatgcctg
    20761 tcccctttgg tactgaaaaa atagatttgg ctgtctcaaa cggtcttcca agttttttat
    20821 ctgttttaga aaacgtttta gaagaacttg aggtacaatc gattgaattg gcggaagaaa
    20881 taaagacaat gaacccggaa atattggaaa aaatcaaatt attgttacca gatacaccga
    20941 ttagctttat cccacatgaa gaaatgaaaa aagagttgaa taactgtcat gcatttattc
    21001 gtacaggcga aatgacacct tacgccaata ttatattagt gagtggagtg gtcttctaat
    21061 ggaaacgaca atgaatcctg aacagatggt actgtccgag cagattcata tgctgattga
    21121 acagttggga ggaaaagaca atattgattc tgtccttcca tgtgcgactc ggcttcgtat
    21181 caaattgaaa aaacagccag tagccaatgc agaaaaatta aaacagcaaa tcccttccat
    21241 taagtatttc attttactcg atagatatgc agaaataatc atggatcagg gattggctcc
    21301 tgcttacaag gaattgacgc gtcaaatcga tcattcagct ccgaaagaaa agctaaatcc
    21361 gatcgaaaaa ctatttggtt tgatatcgga aatctttacg ccgatcattc cgctgtttgc
    21421 cggttctggt atattaaaag gtctggttgt tttagcgact acgattggct ggcttagtga
    21481 aacaagcgga acttatcata ttttgtctgc tgcgtcgaat gccgtctttt attttctgcc
    21541 aatctttctt gctttttctg ctgccaaaac gtttaaagtg aatggatata ttgcggctgt
    21601 cattgctgca gcactgattg aaccgaatct gacctcttta ctttcagaca gtggcaaagc
    21661 agctactgat tttctgggga ttcctgtagt actgatgcag tatacgtcta ctgtcatgcc
    21721 ggcgatttta ggtattttct tttattcttt tgttgaaaaa ttcctcaaaa aatatattcg
    21781 cgaaaatatg caattagtat ttgtaccttt actttcatta gttatcacgg ttcctttgac
    21841 gttaatggta ttcgggcctg cgggtgttta ccttggcgaa ggtttagctg ccgcaattac
    21901 ttggatgatg gatattaacg gaccgatcag tggcgcaatc atcgctggtg gttggaatat
    21961 tttggtaata tttggtatcc aatgggctgt aaaccctgtc atgatcagta atatctctgc
    22021 ttatgggttc gaccgaatcg ttccgttgac aggagcagcc aattttggaa tggctggtgc
    22081 agctttaggt gtatttctgc gaagtaaacg ttcaaaaacc cgttcgatct ccggttctgc
    22141 ttttgcttcg attttacttg cgggagtgac cgaaccaact gtttacggaa ttgcgatccc
    22201 attgaaaaaa ccatttgttg ctgcttgcat tggtgcagct gctggtggag ctgtaatggg
    22261 atttgcccaa gtgaaagcga tcgcttttgt atttggtagt ctgaccacat tgcctgcatt
    22321 catttccggt acttttttct ggtacttagc aggactggct gttagtctag tcgtagcaat
    22381 gatcactact ctcgttagcg ggtttgatga agacctaatg tcttatgaat agttaatgaa
    22441 aaagactgaa gtatgagatg tttatcacat atttcagtct ttttcttttt aggatcatta
    22501 tttttataaa tttttgtgat actttccaaa ctttggtata atttacatat gcataaccat
    22561 ttttatcccc acttgtttat ccccaaacaa aggtggtcta tgcaataaaa tgaacgtcaa
    22621 agaaaaacga atgaatgctc gttgcttatt tacaaattgt tttatttaga atacacagac
    22681 agatttatga aaagaaaaag cttttcgaca cgattatctg ttccaaaaaa tgtctaatga
    22741 atgctgaata agacagaaaa tcaatgaaag gaaaaggact agtttattat gaaaaataaa
    22801 aaaacgatac gtttgctagc tgctacttgg ctgattgctc cgtctgtttt gtcgaccaat
    22861 accgtatttg ccgtagataa tcaaatagct gatcaaacag tcacaacaga tacgactaca
    22921 tccagctcag ctactagtga aacaacggat tcttttacag aagacccaac tggaacaact
    22981 aactcaacaa caggatcgac cgagccaact gaaattgatc cggctgatcc atcagaaaat
    23041 gaaaagccag ccgagacata ttcccttaca attgctcctt ctttgattgg taagatccaa
    23101 ctgactgctt cgacagcgga ttcccctgaa cagcaacctt tggatattac ggaaagtgga
    23161 acggtagaag gtttattagc ggaaacgaag gtaacttaca ctattacgcc agctgaaggt
    23221 gaacaaattg cttcagtcgc tgttaatggt gccaaagaag taaattatga tgcaaagacc
    23281 tttacagtag gtacgaacaa tttatctatt actgcagaat ttaaagctgg accggatact
    23341 tcaacagaga aaccaactga tcctagtgaa gaagtcccta gtacaccaga tccggaaacg
    23401 ccaaacaaac cagatgtggg taataacaac aataataatg gttcaaataa ttcaaacaat
    23461 aataacaata attcgaacag cgggaattct tcgaatcaaa caaacccatc tggatcgaat
    23521 aacaatggaa atactcagag agaaccaaac catccaaaca atggttcatc gaatggcgga
    23581 acgaacaatg cttcatctcg tccaaataca gcaaatcaaa gcggtataga gaatccatct
    23641 tctgcttctt ctgattttgt tgtaaaatca ccaattgatg ctgtattgcc tacaaataca
    23701 acgaatgtcc aacaagcaat cgttcgagaa gcattcaagc atcttggaaa accatatgta
    23761 tggggagcaa aaggtccaaa tacttttgac tgttctggtt tgacttatta cgtatatatg
    23821 aaagcaaccg gtcattatat cggtggctgg acaggcgaac agcaatatgc aggtacgcag
    23881 attccagtaa gtcaggccca acctggcgac ctcgtcttct ggggaccttc ttccggtgtt
    23941 actcaccatg taggtatcta tattgggaac ggacaattta tccatgcgcc tcaaccagga
    24001 gataaagttc gagtaacttc tatttcagat ttcacgcctg attttgctgt ccgagtaaac
    24061 ttagcaggtt tacctgctgc ttcaggttcg ctgacaggcg gctcatctat tttagatgga
    24121 ttagatggct cgttccattt cagtcaaaac caaacaactg atcagttctt gaaaaagatt
    24181 gcagatgatg cacaggaaat cgggcaaaaa gaaggaatct acgcatcagt catgatggct
    24241 caagctatcc ttgaaagtgg atctggaaac agcttattat caagcgaacc aaatcataac
    24301 ctttttggta tcaaaggatc atataaagga agcagcgtga ccttcaatac acttgaacaa
    24361 gatagttccg gacaaagcta ccaaatccgc gcacaattcc gtaaatatcc ttcttacaag
    24421 gaaagtttgg aagactatgc cgacttgatc aaaaatggac tgactggcaa tcctgatttc
    24481 tacaaaccaa cttggaagtc ggaaacaaaa gactacaaag aagcaaccaa atatttggaa
    24541 ggacgttatg ctacagatcg ccaatattca caaaaattga acgcgatcat cgaagcgtac
    24601 gacctgacaa aatatgatga accgaaaaaa gaagcagcaa caactgaaga agcaaacgct
    24661 gaaatcctca atatgtctaa gcgattcgat gtgccaatca aatggacaac agatggcgtg
    24721 ccaatcacag gattagaatt ttcaacgata aaacgcgtca acaacgcttc ttccatcttc
    24781 cgtgtcgctt caatctggga tttgtggaac aattttacgg caagacagat accagaaaca
    24841 actactcgta cagttaaagc tgcaactgtt cctgtactaa ccttgttaag tttagacaaa
    24901 tcattctggc ttgatctgcc tttaaagaaa tagaataaat taaaaagggg atgtgacact
    24961 gtcacactcc ctttttaatt tattctattt ctttatattt attttgtatt ccgttacaaa
    25021 ctcgcgtact tggttaggtt ccagagtgac atctccaaaa ctttcaagtc gctccgctcc
    25081 tggcagttct tgagtctcaa atgtgatacc gccatgattt actaatttat tcccgcgcat
    25141 ttccggtcca tcttctccaa aatttgctgt aaaaatcacg attcctgaag catctgtttt
    25201 tacttggatc gatacttttt catctgggct tgtcaattcc gcagcattta gtaaaccaga
    25261 ttgttttttc aacaaaaatg gatgatcgat gccgcccact atcattgatt ggcgaaaatc
    25321 actggcaaag acctcaccta attttttagg acgttggaag tcaaatgctg tgccacttac
    25381 tgaaactact tctcctgtag gcgttgtatc tttattgagc gtagcaaata atcggctatt
    25441 caaccaaagt atatgttcgt cgattgactg tgctggatca ccggttaaat tgaaatagac
    25501 atgatttgta ggattgaata ctgttttgcg gtcactgatc ccttggaccg tcactcgcca
    25561 tgtatcatcg ttcgtcagcg tataccgtac ttctacatct aaagttccag gaaatccgtt
    25621 ttccatatct gggctagtct gatagaaaat gaccgatgct tcgttttcac cattgatgac
    25681 tgtataatgc cactgcttgg cttcaaagct aggtgcacca ccatgtaaac aatgaccagt
    25741 gtccgggtct gtttttactt cgaacgtttc atcatctaat ttgaatgtgc cattcgtaat
    25801 ccgtccagcc atacgtccga ttgaagcacc gatataaggg tctttttcta gatattcttt
    25861 tgcttcatca aaacctaaga tcaactctcg tttttcatta tcgattattg tttgaaaatg
    25921 aacaacccgt gccccaagat cactgacaga caaagatgtc ccattttgat tcgtgatcgt
    25981 aatcaagtga tagttttcgc caaaattttg ttcattgatt tccatgttcc ttgcaccctc
    26041 ttttctttca tttgcactga ttccagtata catggaaacc gttttcttaa cagcttattc
    26101 cgtgagtaac gagaaaaaat ttactatatt ctaataaaag accgtgaaaa aaaggttgtg
    26161 acaaaaatat tgtcacaacc tctttttccg aacaaacggt gttcaaaagc ttgcatctgc
    26221 ggtaataagc ccaaccaaac gaaaaatatg aggagctatt tttgcttggc tgttcttatt
    26281 acttgatgca ggacagcttt ttcacaatct ctttttcaat acgattccag tatataacca
    26341 tgtcgtcagt acatagtaga tgaaatacaa tacgaagaat atcgtaaacg gaacccaaat
    26401 atctttgtat ggattttgca tgaatagacc aaacatttgt aatccgaata agacatggac
    26461 tacacctaag attcctggtg ctaagaataa cacgcctact tctctacgga tcgattgttt
    26521 aagcaacccg cgtcgtgttc ctatcttttc aagcatttca taacgagcaa tgtcactctt
    26581 gcttcctgac aagatcttga acatcaaaca gctagcaagc atggtcaaga aagctaaacc
    26641 taggaagaat cccataaatt caaaaccaga gaatagacca ttatacactt catacatttc
    26701 atatttctgg ttgctgtact ctactttttg caaagatgga ttgttcattt tattttgagc
    26761 aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct tttacctgaa ctagctgtaa
    26821 agtggattgt ggcaagttca gttgatcaaa ttctgattga ctgactacct tgatttcttt
    26881 ttcttgctgt tccggcaatt catagcttcg aagttcctct tgtacagtca gttcattcat
    26941 ttttttagca ggaacttttt ctttcgtgat cttcggcggt gttccatctt catgttttat
    27001 catgatcaat ggttgagccg caaattcact tgcattatag taaatcgttc ccgcatcttc
    27061 tttttgagta taagaagcag aaagtgttgg tgaaagttct ttgatttttt gctgatcttc
    27121 tgcccgagga ttattcatca ctagatcata tccagaattg ttttctgtca agtctgcaat
    27181 ttcattttta aaacctaatc ctaccgtcaa agcgcccagt gccaaggcaa acagcatagc
    27241 gaccattgag agcatttgtg tatattcacg aatacgaaag tttaactgtg acaatgtgaa
    27301 attgttcaat tttttcaatg aaatctgttc tgatttcttt agtaatgata aaaagaaaat
    27361 gatcacagaa tggaaaatga aataagttcc taatacgatc gtaactaagg ctacccctat
    27421 accaaagact tgataggtcc ccacgtgttt catcatatag taaccgcagc ctagaaaagc
    27481 aattcccagt actacttcaa ggaaaaacaa gaatttatgc tgcttgattc ttgttggtgt
    27541 ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca atcgaaaaag cattgacaac
    27601 agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg atcaacaacc cgtgaatact
    27661 aaaaggtgta aaatgcgaaa tattgagatc taactgatcc actaataatc ggtggacgat
    27721 acttgtcaat cctacaccga cagctgaacc cactaaagta gctgcgatcc ctactaagaa
    27781 tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt gctcccagca tcataaacat
    27841 tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt gcatataaaa tataaacgaa
    27901 tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg ataactgtag ccgcaacgat
    27961 cgtattactt ttcaaaaaag cttcgttgct tgccattgat tcaaacatgt aaaaaatagc
    28021 agatgccata actaatcctg agaataacac caagtagtct cttaaacggc tcttgatccc
    28081 agtaagtgat aatttccaaa gcatagtcaa tctcccccta ctgttccaag tttccaagaa
    28141 tagatagtat ttctcgatag aaggaatcac ggctttgatc tccacgtttc acttcttgat
    28201 ggataccgcc atccttgatg aataaaatac gttggcaata acttgcagaa aacggatcat
    28261 gcgtcacaag aagaatcgat acatgatctt ttgtattcaa ttcgtccatc gtatctaaaa
    28321 gatcccgggc acttttggaa tccagcgcac ctgtaggctc atcccctaat agaatcgttg
    28381 gttgtgtgat caatgcacgt gcggcggcga cccgttgttt ttgtccaccg gaaatctcag
    28441 aaggatagct ttccaaaata tgagagatgg ataaacgttc tgcaattttt tggacacgtt
    28501 gcttgatttc ttttggtctg acgccttgta aagaaagagg aacagcgata ttttcttgtg
    28561 ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa gccaatctca ttcgcacgaa
    28621 aatctgctag ttgatttcct tttaatgtgg tcacatcttt ttgattgatt cggatatgcc
    28681 catctgttgg tttatctaat gtggataaaa tattcaaaag tgtcgactta ccagaaccag
    28741 aagcgcccat gatcccaata aattcgcctt tttctacatc gaagctgatg ccgcttaatg
    28801 cttgcgtctt tttctcattg ttttttccgt atacttttgt tacattctgt acttcgacta
    28861 ttttgttcat ttctttccct accctttaat tagtttttcc agtgctgttt tcggcacgtc
    28921 ttttttttcg acttcttcat acgaagtgac acctttattt ttgttccagg tgatttttaa
    28981 atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg aagtccagtt ctttttcctt
    29041 accctctttc gtatagccag gcaggatata tctatagttg actgtttctt gtccagagtc
    29101 tgattttgaa actgtttttg taccatctgt cgtaatttgt acatagtatt cttctccacc
    29161 cataactacg taatctgcga ttttcacccc tgcaagtacc acaacgatga aaacaagtag
    29221 accgatcaga agttttttca ctttttcccc ttctctctta ctttatatac tgattataga
    29281 aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct tctttcaatt ttgtcatttt
    29341 ctttaaaaca actaaaaaaa gagttcaaga caataatccg tcctaaactc cttcttccta
    29401 tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc tgtattgata acaggttgcg
    29461 ttcctttgtc tgtaatgatt gagacaagta aattattgat caattggact ttccgatcat
    29521 cggtgaaatt gatttcttgt ccatcttcga tttgctctaa ggccatttga gtgattgtga
    29581 cagctccttc aacaatggtt tgtcgagcag acaaaatggc ttttgcttgt tgacgttgga
    29641 gcatggcact agcgatttct gttgcatagg ctaaatggtt caagcgtgtt tcgattactt
    29701 cgacacctgc tactgctaaa cgctcttgca gctctttttg caactcgtca gacactgccg
    29761 tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt atcatacggg tattggctag
    29821 cgatatgccg aatggctgtc tcactttgga tttctacaaa atcttgataa tacgctacat
    29881 cgaataatgc ctttgctgta tcaacgactt taaagactac tactgctgag atttcgatgg
    29941 ggtttccatc caaatcattg acttttaaaa cagaactatt gaaattccga actttcaacg
    30001 aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa gccattttct cgaatcgtac
    30061 ctaaatattg tccaaaaaac aaaattactt ttgcttgatt aggactgact acagttgccg
    30121 aacttaacaa caataacgtg atcaaccaaa gaaaaatact taaaaaaagt gcccaaactt
    30181 tcgcttgcca catacctaaa tagaagaaaa acaatccaac taaagctaat acgaccaatc
    30241 cgacaatccc tgcataacca ttgatataaa atgctctttt ttctctcatg ctacttcctg
    30301 cgctccttta tccttcaata aatatctaat gttacttttt taattttttt ttcgaatacg
    30361 cgccattaca ccttctagtt ctaactcttt gtcttcagtg atgtaccaac cgcttgcctg
    30421 tgtccctccg gctgcctgcg gtaaataatc caattttgtt cgaacccagt gcttttcttc
    30481 tgtaaaatag tcttctacca agacctctag atccatccct gctgaaagag gcaaatcttc
    30541 ccgaacagat ttaaagataa atcgttcctt cttcccatca tacttcaata ttcctgtatc
    30601 gataatcggt aaagaataat aatccagctc tttcttcacc gtcaataaat gtttgaaaac
    30661 ttcatggtac acttgctcta attggcgacc ttctgaattg ttcgggtaat taaccatcac
    30721 tttttctttg tctggatcat agaaggtttg gcttagttga gtgatataag tatgtaatct
    30781 ttccatttct gtttttaact tttctctttg accttcttct accttcatat actctttcac
    30841 cctgttttct ttttagatat tttctttctt attttactat agttatgtgc gttaagcgat
    30901 gaaaaacagc aaagattgga aggatctttg ctgttttttc ttaaaatatc cagttgagta
    30961 caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa tattgatcga tgtgtcttta
    31021 gcagtgatct ttagcttttt ctgtccttgt tctaacaaag tatttgttat tggctggtag
    31081 tctttttcaa tataagattc accagacgtt tggatagtat agccactgtt ttccatctca
    31141 tgcaaagata aattgccacc gtttaggatg acttttcctt ctttgctgat caaactgtct
    31201 tctagatgga agttgctgtt ttcacctgtg acgatcaagt tatccacatt ggcctcatct
    31261 aatcgaagtg agcctaattc atctttcgca gttattgttc ctcctgtaaa ttcattgaac
    31321 gatacccagc catcatgtgt cttagtattc acttttttga ttgatacttc gttgatatca
    31381 attgcactat tattcccttc gattgtcaat tcagataatt catgatctgc agggacaaca
    31441 atagtgattt gtgcacttgt gtcttctaaa ctaatgattg cacctgttga ttttttgact
    31501 gcattcacag tcaacacgcc atttttctcc tttatttctg gcttttctgt cttatcataa
    31561 gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa ctgtttgatt ttctgcatgg
    31621 atgacgattt tgtttgcatc cttgattttt tttgtctcag taaccatatc gatcaaccga
    31681 ggaccattat cccaagtcag actcttttga gcgcctaatg tataagttgc acctgctagg
    31741 aggccaccag taagcatcag accaataccg gtaatcaaaa aatgtttcaa tctcaattta
    31801 tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt ttgtaataaa acgtcgtccc
    31861 atccatttaa caaagttcat catgacataa aaaagccctt ttgttccttt cagcaacagt
    31921 ggcgaaataa atactgcgct tcctgtggcg aacagaccga caccgataaa gaataatcca
    31981 ctcagaatcg attgtccaaa cacactgatt cctgagatga tcatgaatag gccaacacct
    32041 aagatactga tacataaaac atagatacta aaaatgagcg ctagtaaagc aaccaatagt
    32101 cccccaataa ccaatataaa ccccagtgct agtggaatga ggacaggaga agcaaataaa
    32161 ccgataatga ttaaccaaat caggcgaatt tgtctttttg gatttttcac taactcgttt
    32221 tctgcttgtt cttcttctga aaagtaactg atttttaatt ttaaggcaaa ctgctttggt
    32281 gtaccatatt catcaatgat ctgatctaat gtttttccag aatctaaaaa atattcctca
    32341 taatactgga tcaaatcaaa acgatcgtct tcaggaattt cttgaagatg atccacaact
    32401 tcattcaaat attcattcat ttgtgctctc ctccaataac cggtctaaag aatctcgaaa
    32461 aacaatccat tcatcaataa tttcttctaa atgacgactt cccattggtg tcaacgagta
    32521 atatcgacgg tttctgcctt caaaaggttc atcataggtc tcaagatatc cttctttttt
    32581 caatcttctc aaaaccggat atagtgtaga ttcactgaca gagagacttt cttgtacgat
    32641 ttttgttaag gcatacccat acatatcttc cttgcttaat gctgctagca cagtaccatc
    32701 aagaagaaca gttggaatct ggatgtgcat tacgtcacct tctattctca agtgtaatat
    32761 tttatttcaa tctatattat acaacatata atatagtttc aaaatcagtt tgtcaaatac
    32821 aaaaaaactg actaaaagat ttgatccttt ttagtcagca aaaaatggct tctctttgat
    32881 ttttattata acagtggcat aatccaattt gtccacatcc agctcagtgg catgataacg
    32941 atcattgcag gtatcatgtc agctactggg aacattttca cgttgatcat tctaaaaccg
    33001 gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta agatcatgtc tggtgtagtc
    33061 aatggataaa taaattttgc caataagaac aaaatataaa aaataatgaa ttggggaatt
    33121 gctatcacag aaacgacata ccctaaatta cacgcaaaaa tagctgctgt gaaaaaatca
    33181 aggacagatt tagaaatcaa aatcgtactg tctcctgtca tcccagaatc taaacttcca
    33241 tagatccccg taccacttgc acaaaataga acaatgaccg tcactaatgt gctgataaat
    33301 tcatcgtggg tcataccaag cttttcacta gggaagattt tagaaatagg tacttgcatc
    33361 agcgcagccc ccttatttat cccattacct aaatgaacaa tcaaaccaat cgctgttcct
    33421 atcactaaag caaaaataac agccggcata tttttcatcg gcgcaatgga atagattccc
    33481 atccccatcg aacacacacc aaaaaccatc gtcagctgtg ctttaaaatg ttcacttaat
    33541 ttatttccta ctaatccgcc aattagtccg ccaaacaaaa cggaacctac attgatcaaa
    33601 actccagttg gcatcaaaaa actcctctct ttttctacga agcgaaaaaa atcagaaaaa
    33661 tttctgtaat tattgctttc gctgcaagaa agattgtaac agacagagaa gattacacca
    33721 attcattttt tgtgttaaag tattctttat tggaataagt aggaggaata tgatggatat
    33781 ccataaattc gaagtttttc ttgatttagc cgaaacaatg aatttcacca aaactgctga
    33841 tcgacaattc accacccaag gaaatatttc taaacagatt ctttcgttag aaacagaatt
    33901 agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa ttgaccgaag ctggaagctt
    33961 attgctccca tatgtaaaaa atgtcgtaga acaataccat gctatgcaag aagtgcttga
    34021 aacctatacg aaagacaaca atctttcatt aaatatctta acgataccga caatgatcaa
    34081 ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc catcctgaat ttacagtcca
    34141 gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac gccaatgaac atagtgacac
    34201 gatccacttt gctagaagtt tccaggcccc accaagttcg attgagtggt tgcctactga
    34261 aacagacgat tttgtagcag ttctacctaa aaatcatcct ttagctagta aaaagaaatt
    34321 ggatctaagc gaactgaaaa aggagaactt tttgctcctt ggccccacta cgaacttgta
    34381 tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa ccaaaaatct cttataaagg
    34441 agcaagaatc gacttgatca tcaatatgat tgcaaatgac atggggattg ctattgttat
    34501 ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg attctcccta tttctccaac
    34561 aacagagagt tatcttgcat ttactcgtaa agtcggtgag cattctttgg cttcagatac
    34621 attttgggag tatttaaatc aagaagaaaa ctaagcccta cattttctgt ccatctatcc
    34681 acccaaaaaa agagagcgac gcatgacttt cgctctcttt cttgcatcat gtccttgttt
    34741 caatgacacc tctaacattt ctcgtagttc aataagaaag cagcgatttt tatgccaaaa
    34801 gaaccgtaag aacatttccc taatcacaaa aaaacgacta aaaaccccta aaaaccttca
    34861 atagaatttt taggggtttt attctttttc ctattgttta cttaaccgta tcattgctgt
    34921 cgttcctact ccttcgatac tgctataatc aatacgtcca tcatgcagtt ctagaatttt
    34981 tttaaccagt gaaagtccta gcccattccc agaaatttga cgacttgtat ctccttgata
    35041 aaatttttca aacattcgat ctctggtctc ttcattcatt cctacacctg aatcggtcac
    35101 ttccagtaca atctctgtgg ctgtctcaaa tagtttgata tgaatctgtc cattgatttg
    35161 attgtacttt atggcgttat ccatgatatt cagccacact tggtacaaaa actcttcatt
    35221 gcctgtgtaa tttactgcag caagttcgat atccaattct agctgttctt tctcccattt
    35281 aggctgaaaa aaaacgatta cttctctgat ttgttcatct aatcgatact ctttcttttc
    35341 cagttggata cgctggtttt ccagtttgtt caattttaag atgttctctg ttaattgtga
    35401 aagctgcgtg atactttcga taatccgata taaaaatatt tggcgttctt cgtcagagag
    35461 atttggggct tgtaacaatt gaacatatcc ttggattgtt gctagtggtg ttttgaattc
    35521 atgggataca ctggacacaa agtcattcct cagtgtctcg atactattga gttcttggac
    35581 cataacgttg aaatctttat acagttgctg tacttctgct actttctgct gttcatccat
    35641 tcgaatcgaa aaatctcccg ttgctaccag actcatattt gtccctagcg cactgattgg
    35701 atgcaggata cgccgcccaa caaaaatcga tattcctgta cccacgacca tactgaaccc
    35761 cgcaaagacg aataacgggt ataacgttgg ttttccagcc gattcagcag gcgagataga
    35821 atgtcgagac aataagaaaa taaaaccgag aaaacacagc agtgtcacta agatcgaaat
    35881 gaagacaata ctagcaaaat agatccataa ttgtgaaaat agcttttttt tcatctttaa
    35941 ataaccgcct tatagcctag tccccgtact gtgatgattt gaaaatcttc cgtcttttca
    36001 aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc gttcttctgt attcgtatct
    36061 aatccccaaa tatcatccat caactgttgg cgagtgaaga tcttgttggg ataagacagt
    36121 aatttataca gaagcagaaa ttctttttga ggcagaatca acgaatgctg atcttgtaac
    36181 actgtataag catctagctc taattttgtt tgtccgatta tcaagctact gctgtaattg
    36241 atctttgaac gtcgaagcaa tgcttccaca cgtaggatca tctcattgac atcgatgggt
    36301 tttgtcatat aatcatctgc tcctaatttg aacccgcgtt tcttatcctc aaaatcggcc
    36361 tttgccgtaa tcattagaac aggtgtgtca aatctggtgc tacgtaatga gtctagcaag
    36421 tcatagccat ccattttcgg catcatgata tctgtaatga ttaattctat acgtgtcgct
    36481 tctagttttt tcagcgcatc ctctccattc aatgcggtga tgactgaaaa gcccactcgt
    36541 tccaacacag aacggtaaag caactgtaat tgcggatcat cttctactac taaaatacga
    36601 ttcattgatc tccctctgtt ttttgaaaaa tttctagatt ttttcttgta gttcatcttc
    36661 agttaacatt tatttcgtat actacgatca ttgttctgat atacttatta gtataccgaa
    36721 tatttcaata cgggagaagg gtgattatgc ttcaattaaa agatataaaa aaatattata
    36781 aagtcggcga aaccaccacc aaagcattgg atggtgtgtc tgtcgctttc agacaaaaag
    36841 aattcgtagc gattttaggt cctagtggtt ctggtaaaac gactatgctg aatgtgattg
    36901 gtggactaga taattatgat tccggtgata tggtaatcaa cggaaagtct actaaagact
    36961 ttaaggacag tgactgggat gcctaccgaa acaactctat cggtttcatt tttcaaagtt
    37021 acaacttgat tggtcatcta ggcattattg aaaacgttga acttgggatg acattaagtg
    37081 gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc attgcaccga gtcggcttga
    37141 caaatcatat gcataaaaag ccaaatcagt tatctggtgg acaaatgcag cgagtggcca
    37201 tcgctcgagc actagccaat gacccagata tccttttgtg cgatgaacca accggagcgt
    37261 tagatacaga aacaagtatc cagatcatgg aattgatcca agagttatcg aaagaaaaat
    37321 tagtgatcat ggtcacacat aatcctgaac tggcccatca atacgcagat cgaatcattg
    37381 aattttctga tggtaagatc ttaacagatt cccatcctca catcgaacga ccaaaagatg
    37441 atcaatttaa cttacgcaga acaaagatga gtttttggac agcgttaaag ctttccttca
    37501 acaatatccg aacaaaaaaa ggacgtactt tcttgacctc ctttgcttcc agtattggga
    37561 tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt ccaaaaacaa atcgataaca
    37621 cgcaagctga aacgatggcg aaatttccga ttactatttc taaggtaacg actaaccaga
    37681 ctcgtgacga tgccggattg ggtgcaagca aagcggatta tccagacagc aaaacgatta
    37741 ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa taagattgat caaacatacg
    37801 tcgattacgt aacagacatc gatcctaact taagtaataa tatcggattt acccgtacta
    37861 caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca gcctgtcagc ttctcgaatc
    37921 aaaatcctga tacggaatct ctttctttat ctagtacgat gtcagcaatg acaggtgtgg
    37981 gagtgtcaag tttccctaca cagcttgaca ctagcaaaga aaacttttta aaagacaatt
    38041 attctttact tgccggaagt tatccagcct cagcaacaga tgtcgtgctg atcgtagacg
    38101 gcaataataa tactaatatc aacgcattga agaatcttgg atttgatgtg aaagaagacg
    38161 aaaaacttga ttttgatgat atcgttggta caacattcaa attagtaaac aacaatactt
    38221 actatacaaa acttccaact gggaatttta tccctaatac agattatgat gcgatgtatc
    38281 agaatgcttc tgatgaattg aagatctcgg ggatcctacg cgtgaaatct tcttctacaa
    38341 tgaatctctt atctccagga atcgcttata gcgatcaatt aacgactcaa atcgtcaacg
    38401 aaaacaaaga atcagaaatc gtcaaagctc aaagagacag tggtgtaaat gtcttaacta
    38461 ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag ttatttaggt ggagacagct
    38521 tgccttcaag tatcatgatt tatccgaaca attttgaaga caaagaaaag atcttggatt
    38581 atctagatga ttacaataaa ggcaaatcag atgaagataa aatcatttat actgatttag
    38641 ctggaacgat gactgaactg actggcggat tgatggatgc tatcacttat gtccttattg
    38701 cttttgcagg gatctcttta gtaacaagta tgatcatgat cagtatcatt acttatacat
    38761 ctgtgattga acggacaaaa gaaatcggtg tactaaaagc tttaggtgca cgtaaaaaag
    38821 acatcactcg cgtatttgac gcagaaacgt gtattttagg tatttcttct gggatattag
    38881 gtgtgtttat tgcatggcta gctacgttcc ctatcaatag catcttatac aacatgacgg
    38941 atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat tattctagtg atcgtctcta
    39001 ctatattgac gatgcttggc ggtcatcttc ctgcacgaat ggcagcgaaa aaagacgccg
    39061 ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat ttagatttga gtcataagag
    39121 cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat atgttccttg tttcgcgctg
    39181 ttttttttaa ttatttttta taattctggt atacttttct ttcctttgta ccacaactct
    39241 tctggcgtat acctgtcttt tccatttatg aatactgttc catctgcttc aatcgttact
    39301 tcagtcccaa tccacatatt acgagaaagt gcgagaagat tttttctttt tcctcttcct
    39361 ttttttccaa attgcaaggc atcggttatc tcttctgtcc aatgagattt cacgtatttt
    39421 acttctccgt tttcaaatgt aactttcaaa atattcgcta tattttccac ttttactgct
    39481 ttaggaaatg actcctccat ttttttcact ttccttcctc caacactaag tatgcgcttt
    39541 cttttagtga taagtatatc atagtttatt ctattatcaa aaaaacgcat ccattccatt
    39601 gtagaatact ttaccgaaca agttgaattg ttttcctctt tttcaccttt tactatagat
    39661 aggtaaagac aactaaggag gaatctctca tgacagatca accgtatgat ttaagaaaag
    39721 tcatcgaaga actaaaaaca cttccaggtc aatatcacga aacagatatt gaaatcgatc
    39781 ctgaagcaga attatctggt gtttaccgtt atatcggtgc tggcggtaca gtaaaacgtc
    39841 cgactcaaga aggaccagct atgactttca acaatatcaa aggtttcccc ggcacacgtg
    39901 tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga aattttgcac catgattatc
    39961 gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc aatcaaacct gtgaaagtag
    40021 acaaagcaga cgctccagca caagaagtcg ttcatctagc taccgatgca gacttcgata
    40081 ttcgcaacat tttgcctgca cctacaaata ctcctgaaga tgctggtcca tacatcacaa
    40141 tgggtgtagt cttaggatca gatccagaaa aaacaatgag cgatgtaacg atccatcgta
    40201 tggttctaga agataaagat acgatcggta tgtacatcat gcctggcgga cgccacattg
    40261 gtcacttcca aaaacaattc gaagccttga ataaaccaat gcctatcact atcaatattg
    40321 gtttagatcc agcaattgcc attggcacga ctttcgaacc acctaccact ccacttggct
    40381 acaatgaact atggtgtgca ggagctttgc gtaacgaacc tattcaaatc gttgatggta
    40441 tagcagtcga tgaagtgggt attgctcgtg ctgaattcat cattgaagca gaaatcatgc
    40501 caaatgaaac aatgcaagaa gatatcaaca cgaactcagg aaaagcaatg ccagaatttc
    40561 ctggctatac tggggatgct aatccagctg tacaagtagt gaaagtcaaa gcagttactc
    40621 accgaaaaga caatccaatc atgcaaacaa cgattggtcc cagtgaagaa cacgtatcta
    40681 tggccggtat tcctacagaa gcaagtattc ttgccttggt cgataaagct attccaggaa
    40741 aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa attgatgacc atcatgcaaa
    40801 tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca agcagctatt cttgctttat
    40861 cagccttcaa agaattgaag acagtcattt tagtcgatga agatgttgat attttcgaca
    40921 tgaacgatgt agtttggact ttcaacactc gtttccaagg agataaagat atcatcgtga
    40981 tcccaggaat gaggaaccat ccattggacc catcagaatt accagaatat aatcctggct
    41041 ctattcgttt ccgtgggata agtgcaaaaa ctatctttga cggtacagtt cctttcgata
    41101 tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaga agattgggaa aaatatttga
    41161 aataatgaat gatctgcaac tgttttgaca tcataaaaag gagtgcggaa aatggaacaa
    41221 ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg gcgcttcagg caccatttat
    41281 gccatcaacc tagtgaagaa actaaaagaa tatccgatga tagaaacaca cggaatcatc
    41341 agtccatggg caaaacagaa cttaaagctg gaatccgact tatcactaac tgaattcgaa
    41401 agcctgtttg attaccatta ttcaaacaaa gatttaggtg ctgctgtcgc cagcggatca
    41461 tttttgaccg atggaatgat catcgtaccg gcaagtatga aaacagtcgc aggtatctct
    41521 gttggtttag gcgacaacct gattgcacgt gctgctgatg taacaatgaa ggagcaacga
    41581 aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca ttcacttgga aaacttgact
    41641 aaattagcac gactaggtgt gcaaatcata ccaccaattc ctgcttttta taatcagcct
    41701 caaacgatcc aagatcttgt taatcaccat acaatgaaac ttttggatca atttgaaatt
    41761 gtaaatgagc attcaaaacg ttggcagggt gattaatatg gaaaaagaaa cgatagttga
    41821 agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa gtaatcggta aagacttatt
    41881 gatcacttta acaggcggag atacaccaca tatcggaaca gtcactacat tttctcgtga
    41941 tacagagatg caaactgtac gtttccctag ccacgatggt cggttccata aagacgatgt
    42001 cttagccgaa aaaatagctg tgatcatcaa aactgccgtt ccgggaagtt gtacgatcac
    42061 ttccggtatc catgttgacc atatctccaa tgcacaaatc aacgcctctt ttccgatgtc
    42121 agaagagtta ggttacaaga ttttagattg gttggaaaaa atagatttcc atatcaaaga
    42181 accagtttat tataaagatg gtgaaaaacc attatgaaag aagaggttgt gaaatcagcg
    42241 ttatgacctg agtatgagag aaaaaaatgg aaaaatagct tctcatattt tgccgttttt
    42301 gagctaatac cgagggacag ctgattgaac gccgtttatc aagaggttgt gataaagccg
    42361 cttcgtttcg agtagtaaga aaaattttgt aaacatgggt ttccacattt ttgcataaat
    42421 ttgacttact accgagaaac tggcttttga acaccgttta tcaagaggtt gtgacacgtc
    42481 ttttgtcaca acctctttta tttttgcttc actgtaactg ctacaatatc accgatttct
    42541 tttccgatgt ttttacgaat atcttttcgt agtccaagga tgtagcagac tgaaccatct
    42601 ggatttttca cgcccatatt cacgatactg ccttcatacg gcacattgtc aaaagttgct
    42661 tggactttaa ctcttccagc accgaattct tttcttatat catacggaaa aataatatat
    42721 gccccgccct tgccgacttc ggaagcatag atttttgatt caaaagaata aatttccctc
    42781 attttatttc tcctattttt tacatctagt atagcatagc aaaaaaaaga aggaactgtg
    42841 acaaaagtct tgtcgcagtt ccttttttcg aataaacggt gttctcctgc caactcctcg
    42901 gtcacaagtc acaatagtca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    42961 ctcagagtta aacggcaggc tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    43021 tacggcacta agcccaatca gatgaaaaat atggagtgct atttttatct gattgttctt
    43081 actgcttgat gcagagcagc tttttcacaa cctcctacat ggtgttcaaa atattattcg
    43141 ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt ggaattcagc agctaaggct
    43201 ttcgtacgtt gttcttgctc tgctttgctc atttttggat tttcagtcat tgcttttacc
    43261 ataccgtctt gaacataagc ttttgcttca ctttccatcg tactttgctg tttcttcact
    43321 aattcagcta acttggctgc ttggtccgta cttaattcta cccatgtctc tgggattttg
    43381 aatgtattca ctcggctgtc atattcgtga ttgttatcag caatattgaa ccagaattta
    43441 tacatgtcgt ttttgtattc ccaataagta gttttcgtca ctttttcagc atcgccgtct
    43501 gtcttttcta ctttatttgt ttcgttatcc gtaccagttg cttttggttt ttcttgtttt
    43561 tcatttgttc ggtaaagata cactttttct tttccattcc ctaaagcttt atacaaaagc
    43621 atatctgctc cttgggaatc agcactggaa acaatttttt ctgtttttgt ttccgttact
    43681 ttttccattc caaagtgatt ggacaaatta gcgacgatca aacttagtga agcgacgaag
    43741 attagtccga aaatcaaaga taaaaaggtt tgccatgttt ttttagcgaa cacgttcgtt
    43801 aaagcaaaag caagcaagct gacaatcaaa actccaataa tcatgcatta tcccccgctt
    43861 tcattagaac aggttctttt ttcttcaata aaaaggcaat aataaagcca agaatcccaa
    43921 agatgatcgc tacgataaac gcggcatggt aacctgacag tgtggcattg attgcctgat
    43981 ctttataagc taatggcgta ttatccaata cagatttgct tgggatattt gcttttgtca
    44041 cattcgtcaa cacactgatc aaaatagctg taccaatcga gcttgcgatt tgacggaatg
    44101 tattatttac cgctgtccca tgactgatca agttatttgg caatgcattc ataccagaag
    44161 tcgttaccgg catcatgacc atggagatcc caaacatacg gatcgcataa aggatcacga
    44221 tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg tgcagttgcc gctgtcaaaa
    44281 tgaacatccc tgtgatggct aaacgtttcg cgccgtattt atcgaaaatc gcacccgtca
    44341 caggcatcat agcccccatg atcaatgcac ccggcaacaa catcaagcca gaatggaatg
    44401 ctgattctcc acgaatattt tgaatataca atggtacgac catttctgcc cctaccatcg
    44461 ccatatttgt tacaccagct aaaacagctg caatcgtgaa tgtagatgat ttgaatacac
    44521 gaagttccaa gaatggatgt tccaaatgta attgacgcca tgagaacaag gcgatgaaaa
    44581 tcacaccaac aactaagaaa ccaattactt tcaagcttcc ccagccgtca tctcctacac
    44641 tagaaaatcc gtaaagtaaa ctaccgaatc caatcgttga taaaatagcg gataaaatat
    44701 caatgctagg atttgacaat ggaataacac ttttcattag gaagaatgct aaaaccaaga
    44761 caagaacaac gattggcagt accataccaa acaagtcacg ccaatggtag ttatcaatga
    44821 tataaccaga taatgtaggg cctaaagcag gagcaagtcc aataacgatc cctgacatcc
    44881 ccattgctga tcctcttttt tctggcggga aaatagatag catgatattt tgcaataatg
    44941 gcatcgatac tcccacaccg caagcttgga tcaaacgacc gattagtaat gtactaaaat
    45001 taggtgctgt aaagcaagta atcgttccga tcaaaaagat cgtcatggca gaaagatata
    45061 aatttcttga actgaattta ttgatcagcc atgctgttat tgggatcatg atcccgttga
    45121 ctagcaagaa accagtcgtt aaccattgca catcagacgc tgaaatatcg aaagctttca
    45181 tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa aacggtacaa aatgtaccaa
    45241 ttaataagac aacaactaaa agtgtccgat tatatggttt tccgtaaata tctacgggtt
    45301 ggttgttact catatttctc cttctttctg aattttttct gaaactacta gaatacttta
    45361 gtcctaaatt cgctatttgt caacctactt gacacataag ttaatacgga tataattaag
    45421 ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg cttcccttaa acgtttgttg
    45481 gaaaatgatc aattggttgt tggcatcagt gaattaagtg atatcgtagg tgtgtctcca
    45541 cgtcaattaa ggtattggga acaaaaggga ttcattcgtt cgatcgaagc tgatgcgaat
    45601 tgtcctagaa aatatcgttt acctacagtc ataaaagtag aactgatcaa aaaatattta
    45661 gatgacggat ttactttgtc aaaagctgca gaaaaggcag aaatccgcca aaagaaaatg
    45721 caccatgtcc gtaaagtttt ttcaaaagca atcaaagata tcgaattgat cgacgaacgc
    45781 tatacgattt tacaaatcgg taacttcaaa gaagagaatg ctaagatgta catcattcat
    45841 gacaacgaga cagacgaatt aacttacaag ctccttcctg tcgatgaaaa agctgactac
    45901 gaacagttgt taaaagaact ctaaacgcca ctaatctcct aagtaccaag ctttttaata
    45961 agtagatgct acataaatac ttgaaaaaag gcctgtttga acataagaat atccgaacgg
    46021 tgatctgcgt ttctttcata tagaaaggaa ttctccatca ctgttcggat atttttttat
    46081 ttttttcggt tactttcttc taatacatct gttagccaat ttgcatattc tgtttcccga
    46141 ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt catgtttttc tttttcagta
    46201 ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt tttcttcatg aaggtcacgc
    46261 tgctgctgaa taatttctac taatgccgga tcattcacgt ctttgacgaa atacaatttc
    46321 agaacaaatt cgtctcgatt aaccggtagt tcattggtgg gtgtatgaat ccactctgcc
    46381 aattgttcct tcccttcttc tgttaattca taaactttct tttccaattt ttcacctgtg
    46441 atctctactt gatgctgaat gattccttgc tcttccattt ttgcaagttc aggataaatc
    46501 tgactatgtt ttgcttgcca aaattctcct atttcatttt tgaacgattg attcaattca
    46561 tatcctgtca tcttcttctt acttaataac cctaacaaaa tatatcgaag tgtatttttt
    46621 cttgccatca gttttccctc cttgacaaat ctttaaatcc attctatcat aaatgagtaa
    46681 tcgaaaacat gtagtttatt acatgtttta atttataagg agatgcttaa acatgaaaga
    46741 atttaaagaa ttggaagatt ttattggaac tcactttatc tatacgtatg acaatggttg
    46801 ggaatatgaa tggtatgcca aaaatgacca tactgttgat taccgtatcc atggcggaat
    46861 ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc gttaaattga cggacggcgt
    46921 attcaaaatc acttggacag agccaacagg gacagacgtg gcattggact ttatgccaaa
    46981 tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg gttgaagaac atccagaaat
    47041 cactgttact ttccaaaacg aacatatcgc agaaatggaa gcagctcgtg aaaaatatga
    47101 aacttatcca aaattacttg ttcctgaatt tgctacgatc acttatatgg gtgatgcagg
    47161 tatagacaat gaagatgtta ttagcgaaga accatacgat ggaatgccag atgatatccg
    47221 tagcggaaaa tacttcgatg aaaattataa acgagtaaaa taaggttgtg aaagagaggt
    47281 tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa cggaaaaata gttcctcatg
    47341 ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcaagagg
    47401 ttgtgaaatc agcgttatgc tttgagtaat aagaacgcta gttagaaaat agcttttcat
    47461 attttctagg ctagtgggct tattaccgag aagcagccgt ttgaacaccg tttatcaaga
    47521 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaatggaaaa atagttcctc
    47581 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaaa
    47641 gattgtaaca actgcatatt cacacagtta aattagtgac ttcaaaatga aataatgctc
    47701 ccagcaaaaa attaggctgg gagcattatt tcgttattct gttcatcaaa aatcagcgtt
    47761 ttgattccat ttcatcacaa gttcctcctc atttactgct tcatcttttt gctgtgtttg
    47821 aattgtaaaa ccttgtgaaa gatagaaatg aatagctgat tgattttttt gatagactgt
    47881 caaagtcagt gttttattgg tttcttttat ttttttcatt agttgcgtac cgattctttg
    47941 ttgccggtat tcccttttca caaagattcc tgcgataaac tgatcaatca ccccagcaaa
    48001 accaataatt tcttgatcta tttctgctaa gtaaatcact gcatctggta aagctttcct
    48061 aacagattct acttgattct cccaataagt tgatggaatg aattcatgag aatccaagtt
    48121 agcatgcagc caaatcgata gaacagattc cagttctttg gcagacaatt ttgttgttgt
    48181 gcggatcatt ttttctctcc ttacttattc attgatttta aaataaaaac catttttttg
    48241 aagctctttt acaagttttc gcccctcaaa aaagtatatc ttctcttgtt ttacttttct
    48301 aataccaaaa gaaacagcaa ctttaggatc atcagctgaa acgattcgat aagttccacc
    48361 aggcaattga tccagcttga ctttcccatg atggtcgctt tgtactgaaa acggcacacc
    48421 atttctagta agtgtttttt tcttatgttt caaaatgaat gaactaccat ttaccggttt
    48481 ccctgacgaa tcactaataa taaatgaaaa atcaagcact tttttactta atctatactg
    48541 atagattagt cgtccgataa acactaacag tatgactaca gctattatcg ttcctgcaag
    48601 aataaaccat tgccgcatgg ttctttcctg atttcctttt ccttcttctt tttttaccga
    48661 ttcgttataa ggaatccgat gtcctgtcac tagcaaacga tgcgtgttga tcatatacgg
    48721 cgtacatgta agcaaggtaa ctaagtcttc tccttctgaa atcgttagaa cagacgtgtc
    48781 atctggcagt accacttgta tatttttaac ttgataagcc aatttctccc ccaaaacagt
    48841 caaaataaac gtgtctccgt tttctaaacg atctaattcg gtaaataacc gtcgattagg
    48901 taaaccgctg tgagcagaga tcactgaatg cgtcgaattt ccaccaagcg ggaaagaggt
    48961 tccttgcaag acgcctgctc cattttcaag gatcctacca ttgagccgat cataaagtga
    49021 aatatttaat tggatcttag gtatattgat acttccgatc agccattcct ctaattggct
    49081 ggatgttgta ctttcatttc tgttcttttc attgaaagga tcagtaccag gattgaatcc
    49141 ttggtcagtt aatcgttggt ttttctcctc cattttcttt cgctgcactt caatttcagc
    49201 tgcggtccgt ttttgtactt gttcgatacg tttttgatca ataaaactat ttaaagaatc
    49261 cacataaaat ggatataaag cgatcagtaa ccctacgacg atacatatta ataaaaacaa
    49321 acgaaacaaa agtcgacgag actgtttttg cttactctgc ttcacatgtg tcctcccttg
    49381 caagtttttt tgttctttct tttattatat gggttcagga aaaaaataga aaatgaaagc
    49441 cgatgaggtt gtgaaaaagc tgtcctgcat caagtaataa gtacagccca aaaaaatagc
    49501 tactcatatt ttttgttggg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    49561 taaggaagag gttgtgaaaa ctgcgttatg ctttgagtaa taagagcgct agtcagaaaa
    49621 tagcttctca tattttctag gctagtgagc ttattaccga gaagcagctg attgaacacc
    49681 gtttaccgag aggtcatgaa aaagctactt tgtttcgagt attaagaaaa attctgtttc
    49741 aaaacaagga gatgcaacaa aacttatgtc gcatcctctc tccttagaac tttcacttat
    49801 gtttttcttg taaaattcat aaatattttc atgaattttc taatttcttg cctacctctc
    49861 gcataggata tatgatgaat ttatctaaag tttgcaaagg agttcgacaa ttgaaaaaat
    49921 atggcccatt tttagcaatt tttttcattg ctttggcatt tctcatgcct catttattca
    49981 cacataaact aattttgggt gctgatgccc cattccatta caatcggttc tatgatgccg
    50041 cgcaacaaat taaatatggg gatttccatt attttccttc cctttatgga ttccagcaaa
    50101 gtggcaggat cgtgaatgcc gtttacggcc catttttcgc ttatttacaa ggattgatcg
    50161 tgttgcttgc aggtagctgg tttcattatc agattctttc aaatttcatt ctttatctta
    50221 tttcaggatt ttctatgctg attttactga gatatgtcaa tgttcgaaat tgggtaagtg
    50281 taccagtagc cgttttattc atgagtacgt attctattca atactggtct atcaaccaag
    50341 gattcactag ctgggggact tgtttttatc ctttatgtat gatccctatt gctgatttcg
    50401 tccttcataa aaagttccct gttatcaaag tcgctgtctc agtgggactt atgacacaga
    50461 tccatttact gtcttcgatc atgttagctt tgatgtatat ccctttttat ctttattact
    50521 tttttaacca gactgaaaaa ggaaaagcac tgcttcaact gattaagtcg gtcttgcttt
    50581 attttgttct gacagcaaat atttgggcag gattagctat tgtttacagt gggaataaga
    50641 ttcttcctcc ctttgttaac agagaaatga gtcaaaaggc gatagatctg gatggcagtt
    50701 attggctcca ttaccctaag gcctttccta tattgatatt atcaggtttt ttgcttttta
    50761 ttttctttca acaaaagaac accttattcc aaaaaatcgt gttgtacaca agtcttgttt
    50821 tcttttttct ttctactagc ttgattccat ggacgacatt gatcgaaaaa aatgtgcctt
    50881 ttattagttt gatccagttt ccatttcgat ttttcgtgcc atttacgcta ttgcttctac
    50941 tttaccttgc cctttcactg aaccagtggt ctgaacaaaa gtggttccgt cttttaagta
    51001 tatttggcat tcttattttt tcagcacaga cgatccagaa cttataccag catctggaaa
    51061 agtgggaaaa tgaaacattt gtcagtcgac atacttatct attcgatacg ccggaagaag
    51121 caaggaaaac attcttcagc aaagatatgt ctaccagctt gttcgttttc caaaaaacca
    51181 cacctgacta tctgccaatc tataaggaga caaaagacaa taaatacgac cggtataacg
    51241 aagaaatact agaaaacgaa acaactttta taaaaacaca tccaggcggt cttttgacaa
    51301 tcaagtggaa aaacactggg gacaaaactg ttcatatccc tgttatcgtc tacgatcgga
    51361 ctgtgctaca gcaaaatgga aaaacattga ctgattatga agtaacagat atcgggaccc
    51421 caatcgtgaa gcagcaaaaa ggaatcaacg agctcacgct tcattatcaa acgccaatct
    51481 atttctattt cattctttct cttactttga ttggctggtt taccttgcta tgtttattta
    51541 tttatcatag atacaagctt ttacgtgctt aggcaaaagg gataaataca tccgatcaaa
    51601 aaactctcca aacggtataa tcgtttggag agttttattt taatcagttt ttttatgtta
    51661 tatgttatat tttttattcg ttcacatcta attcgatttc caccccgtag tgatcactga
    51721 tgattggact atcgatccca tcaaaaatcc gttgatattt tttaacctgc attccttcag
    51781 ggacgaaaat aaaatctata cgcaattttt ctgtattttg ttcccaacca tctattttct
    51841 tttccacagt agctgctttt cctttttcat tcgcgacaac atacgaatct tgaagaagaa
    51901 ggcaactttc tcttacagta taataacctt gagagtctac tggattattg aaatcaccta
    51961 ggaaaaaaag tggtacgcga gtttccaaaa agtagttttc caagtttttc cactcaaaat
    52021 aaaagccact ttttttatct gtccaccaag aaaagtggca gctcgagaca aaaatgtcct
    52081 gatcatctat ctttgttttc ccaatcaaga tcttgcgtgt ctgataatct tctttctttt
    52141 gaaattggga gacggtaagg acctgacttt ccaatgtttt tttcgtaagc agtgcatttc
    52201 cttcttcata tttatcataa ccgatgtggc tcatttccca actccaaaaa tactcttttc
    52261 cgtgttcctt taaaagctga actaaacggt aggcaaaatt atctttacgg ataggtactt
    52321 gattctttac cggacagaac ttcattagct tcgcaggttc tacttcttca gattccagta
    52381 attggttgat ttcttgtaaa gcaatcactt cataatcttc taacaggatc ttatctgcta
    52441 attcttgcaa ttttttctct ggttctggct caagccagct atgcgtgttg agtgtcatga
    52501 atttcattgg ttttctcctt gtcttcattt cgaaacaaaa gggttgtgac aaaatttttg
    52561 tcacaacctc tccagcaaat agtgttctcc tgctaactcc tcagtaataa gctcaatgga
    52621 ccaggagcta ttttttgttt ggctgttttt atcacttggt gcagggcagc tttttcacga
    52681 cctctccact aaacagtatt caaactgctg cttctcggta ataagcccaa ccaaacgaaa
    52741 aataggagga gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    52801 caacctctcc ttacatttta atggagccga tcactttacc agctgtcact gtacctaatt
    52861 gcttgatcac tacttcttct actttatcgt ttgtaaatac gatcatgatc gttggatctt
    52921 tttcttcgtt ttggatcaca tcaagatcca tctcagctaa tttcgttcct gttcctactt
    52981 cttggccttc tttgacagca atctcaaaag ctggctgatt catttggact gtatcgattc
    53041 ccatatggat caaaacttcg ataccatctt tcgtttccag cccaattgca tgtttgctag
    53101 ggaaaatact tgtcactttt ccagcgatcg gcgcaacaac ttctcttgat gttggaataa
    53161 cagcaaagcc gtcacccatc attttttgtg agaataccgg atcttttact tcagataaag
    53221 gaatcacttc tccagaagct gcggaaacta gttcttgttc tatacctaag tattgcttac
    53281 tacctgcttg tccatttcct gtagaaacca tttctggctc tggaatctct gctcctgatt
    53341 gcagcagatc ttcgatatct gatttcaaga catctgcttt tggtccataa actgcttgga
    53401 ctccattgtc tttgacgatc aatcccatcg cccctgctcg tttccaggct tcttcagtcc
    53461 ctactttttc tcgatcattt acacttacgc gtaaccgagt catacaagca tcgacatctt
    53521 tgatattttc ttttcctccc agcaaatgaa tgatctggac cacttgctga tctgctgttc
    53581 ctgttccgct gttagataca acggtttctt cactgtcatt gtcgtaattt ccgttacgtc
    53641 ccggtgtagc aaagttgaat tttttgatca agaaatttgc taagaaatac gtaacaacac
    53701 caaaagcgat cacacacaac acgaagttga taagatctcc acctagaccc gctttgatag
    53761 ctaacggtgt ccgagtcagc aattcgatat tcccaaatga atggacccgc aatggcaaga
    53821 tatctgccat cgcaaatgca gccccttgga tcactgaata aatgacatat aatggcacag
    53881 cagcaaacat aaacatgaac tccaatggtt ctgttacacc tgttagaaag acagccaacg
    53941 ctgctgaaaa atacattgat ttatatcttg ctttcttatc aggatcgaca ttacgataca
    54001 tcgcaaaagc cattcccatc aagatacctg aagaaccaat catttgtcct actttgaaac
    54061 gagccggcgt ccagttatgc aacacaaact catattgcga catatcccct gcacctttaa
    54121 gattcactaa atcagtggcc caagccaacc ataacggatc ttgcccaaat acttgtgtcc
    54181 ccgcctgtgc gcctgataaa atctcatacg atccgccaag ctgtgtatag ttgataggaa
    54241 tcgtcagcat atgatgcaat ccaaatggca atagtaaacg ttctaatgtt ccaaataaaa
    54301 acggtgctag aatcggtgcg ctttcttgtg attctgcaat ccataaacca aaattattga
    54361 ttccagcctg tacgtttggc cacaccacag caagaatcaa tgccacgatt gttgaccata
    54421 agataacaac aaacggaaca aagcgctttc cattgaaaaa tgacaaagca tccggcaatt
    54481 ttctataatt atagtactta ttataagcca ttgccccgac aaatccagcg atgatcccta
    54541 cgaatactcc catattcaaa gctggtgctt ccaatacact tgtaaaaaat cctttaacca
    54601 tgatcttcgt accgaacaga gtgtgggtaa atgcatcttc attcgcaagc atgtcacttg
    54661 tgacaccaaa gatagcccca gtgatccgat tgattaaaat aaatgagatc ccagctgcaa
    54721 atgctccgcc tgctctttct tttgcccaac ttcctcctat tgctaaagca aataaaatat
    54781 gaagattacc aataattgcc caaccaatat tttcaattac tccgcccgtt gtgacaagcg
    54841 gcgtccaatc aggattgatc aagggaattg atttccctat actgatcatc aacccagctg
    54901 ccggcataac tgctaccaca accattaaag cctttccaaa tttttgccaa aactcaaaac
    54961 taaatatctt tttcattccg tttagctcct ttacgcaatc ggtttcgtta aacgtattat
    55021 agcgtgcgtt ttcttttttg taaatgcttt attttttatt tttctgtcta taccattcta
    55081 taaaacctat aatacgtgtg ttcccttcta taattttctc ctaaaaattt atttttttaa
    55141 gcttttttct atgaaatatt ttcttttgtc tattgacttg tgcaatcggt tgcgtttatt
    55201 atgaacgtat aaaacagctc attaggagga gaaaaatgaa acagatcaaa cgactatttg
    55261 ctattgatcc atggaaaatc agcacgacac aattagataa aaaaaatctt cgactgcaag
    55321 aatctttaac aagtattgga aacggttata tggggatgcg cgggaacttt gaagaaactt
    55381 actcagggga ccaccatcaa ggtacttatt tggcgggtgt ctggtatccc gataagacac
    55441 gtgtcggctg gtggaaaaat ggttatccag aatactttgg aaaagtgatc aacgccttga
    55501 actttatcgc gatagatatt tcgatcaacg atcagctgat cgacttggct tctttggaac
    55561 cagaagactt ttactgggaa ttagatatga aaaatggtgt attatctcgt acattcacaa
    55621 tcactacttc tactaataaa gtacgcattt ccttcgaacg ttttctgagc attgtcaaaa
    55681 aagaatcagc ttatatctat ttaactatag aaatgcttga aggtagcgga acagtcaaac
    55741 tcgtatcaaa attggatgga aatgttcaaa acgaagacag caactatgat gaacattttt
    55801 ggaatgagat tgatcgtgga actatagaag aaatcggcta tttgacgact aaaactattc
    55861 caaacaactt tgggattgaa caatttactg ttactgcagc aatgcgccat ttcgttaatg
    55921 gaaaatcagt catccctgac tatcacgaag atactttagc attacatggt tgcttgtctg
    55981 cagaattatc tcaaggtgag acatggtcag tggaaaaaga aattatcgtt cttactagta
    56041 gagatattcc agaagcgcag caagcagaaa aagctgtctc agaattaacg aaccagacaa
    56101 acaattatat ccaagcaaaa gaagagcaaa cagcggcttg ggcagaacga tggaagcttg
    56161 ccgatgtggt catcgaagga gacgaagaag cacaacaagg tattcgattc aatttgttcc
    56221 aactattctc tacttactac ggcgaagatg aacgattgaa tatcggcccc aaaggattca
    56281 caggagaaaa atacggcgga gccacttact gggatacaga agcgtatgct gtccctcttt
    56341 atcttgcttt agccaaacct gaagtaacta agaatctgct gaaatatcgg cataatcaac
    56401 tgccgcaagc aatccacaac gctcagcaac aaggattaaa aggcgcgctt tatcctatgg
    56461 tcaccttcac tggtgtcgaa tgccataacg agtgggaaat cacattcgag gagattcacc
    56521 gaaatggcgc gattgcctat gcaatctaca attatacgaa ttacaccggc gatacggaat
    56581 atttgaaaaa tgaaggactg gaagttttag cagaaatcgc acgcttctgg gcagaccgtg
    56641 tccatttttc aaaacgagca aataaataca tgatccacgg tgtgaccggc ccaaacgaat
    56701 atgagaacaa tatcaataac aattggtata caaatacgat cgcagcttgg gtcttgcgtt
    56761 acacgcgaga aaattattta acttatcaaa aagaagctgc tatcactatc agcgaagaag
    56821 aactagccaa atggaaagat atcgcagaga acatgtactt cccagttgac gaagaattag
    56881 gaatctttgt ccagcatgac acattcttag ataaagatct catgcctgta tccgatattc
    56941 ccacaagcga tcttcctctg aatcagcatt ggtcttggga caaaatcctt agatcttgct
    57001 tcatcaagca ggcagatgta ctacagggga tttatttctt caatgatact tttacaaaag
    57061 aagaaaagca aaaaaacttt gatttctatg aaccaatgac tgtccatgag tcttcccttt
    57121 cgccaagtat ccattcgatt ttagcagcag aattaggaat ggaaagcaaa gctgtagaaa
    57181 tgtaccagcg aacagctcgc ttggatttgg ataattacaa taacgacaca gaagacggct
    57241 tgcacatcac ttccatgact ggtagctggt tgtcgattgt ccaaggattt gctcaaatga
    57301 aaactgccca tgatcagttg agattcgcac catttctgcc aaaaacttgg aaagcttatt
    57361 ctttccatat caattatcgc ggacgtttat tgttcatctc agtcacagat gaacaagtta
    57421 ctttcgagtt attagaaggt tctcctctag aaatttacgt ctatgaacag tcttattctt
    57481 tagcggatcg tctagaaata acgctaagaa aggaggaagc aaatgtttaa aggggtattg
    57541 tttgatttag acggtgtcat taccgatact gctgaattcc attatcgtgc ttggcgaaag
    57601 cttggtcagg aaatcggcat tacgatcgac cacacattca acgaacagct aaaaggcgtc
    57661 agccgagaag actcactctc tttgctttta gcttatggcg gaaaagaaca ctctttttca
    57721 aaggaagaat ttgcagaatt agcgaaaaga aaaaacgatt attatttaga gatgatccag
    57781 acgatcgaac caaaagatgt cttccccggg gtcgtgcctt tactggattc tttaaaagaa
    57841 gaaaaaatca agattgctct tgcttctgca agtaaaaacg ggccattcct acttgaaaaa
    57901 atgggtctta caccttactt tgatgctatt gccgacccgg cagaagcaac aaacggaaaa
    57961 cctgcgccag atatcttcct ccttgcagca aaagctgtcg gactaaaagc agaagaatgt
    58021 atcgggatcg aagacgcgca agccggtatc caagccatcc tctcaagtgg cgctcaacca
    58081 attggcgtgg gacgtgctga agaccttgga gaggagatcc cgcttgtccc tgatacgact
    58141 tttcttacga tcgattactt gaaagaaaag tggcatgacc atggataatc cagagatctc
    58201 cgtcgtacca gttccagaga ctgaaataaa gaaaatcaca ttgaagaacg actacttaga
    58261 agtcgttctt ctcaattacg gtgcccgcct gcaccaaatc ttcgcaccag acaaagaagg
    58321 aaaatcagaa aatgttttat tgtcttacga tcgtttcgaa gatgttctaa cagacaaaag
    58381 cttttttgga gcaactgtcg gacctgtagc cggaaggatc cgggatgctt cttggggtga
    58441 gcatagattg gagaaaaact gcggatcaca ccatatccac ggtggcacga aaggctggtc
    58501 tttccaattt tgggaagtag aagttttcaa aactccatca tcaataggtg tggtctttta
    58561 cttaaaagat gaattttcta cctatccagg accaatcact gctacgatca cttaccgttt
    58621 aacaaaaaac gaattagaga tgacaacctc tgctagtagc cttgttgaaa ccatctgcaa
    58681 tccgaccaac catgcctact tcaacctttc aggaaatgga acaagagaca tctatgaaca
    58741 tcagttaacg gtctttttag atggtgtgct tgaattagat caagaaaaac tgcctactgg
    58801 aaattggaca aaaaaagaaa aactgccaat cgactttaga aaatccccaa cactgcaaga
    58861 gatccttgca tgttatccag atggactaga tgatgtattt cttttgcatc atcctaggct
    58921 ttccaaaact tccttacaat tattcgagaa gcattctggt cgccaaatga ctatagcaac
    58981 ttctaataaa agtatggtat tattttctac gactggattc gaagctgatt tctctgtcaa
    59041 tggaaaacag atgcattcaa actacggatt agcaatcgaa ccgcaagaaa tccctgatat
    59101 cgttcatttc cccaaatttg gatcgatcaa cctccatcct ggtcaagaac ggatcagtca
    59161 gacgatttat cggttctctg cacaataaca agagaaaggt gtttttatca tggctattac
    59221 cgtaaaagat gtggcgaaaa aagccggtgt tgccacctcg accgtctctc gtgtcatcaa
    59281 cgatcatcct agtatttccg agacaacaaa gaagaaagtc cgaaaaatta tggatgaact
    59341 aggttatgtt cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg
    59401 cgtaatctta ccccctcttg attcaaaaga gcggatcggt aacccatttt atcttgagat
    59461 catggaagca atcaacgaag aagcgcggaa ttatgatatg actacagcaa tcgctactgc
    59521 taaaaatttt gaattgctgt tagagaacgt ccagcgtatg catttacaaa aacaagtaga
    59581 tggttttatt ctagtgtatt cagatagtca agatactgtc attgattatt tataccaaaa
    59641 ccacatccct ttcactttga tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt
    59701 agataatgat aaccagcttc taggtaaaca agcaacagaa tttttgatcc agaatggaca
    59761 taagaatatc ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg aacgatattt
    59821 cggttatcaa aaagcaatga tgatggctgg tcttcctgcg ttatccagtg tagtactaga
    59881 gcatccggag gattatctgt cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt
    59941 ggtggtcatt gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa tgtatggcta
    60001 tgtcattcca gagacactct ccgtcattag cttcaacaac tcgatcttct caacattaac
    60061 acacccttac cttacaagta tcgatatcga tatctccgaa ttaggacgtg tcgctatgca
    60121 aaagctgcgc gaattgatcc atgaagaatt gagcagtggc gtacgtttgg tcatccctca
    60181 tcgcttgatc aaaagagaga cagtcgttcc tttagaaaac ccatactaaa tagctgactc
    60241 actaaatacg ctgaccattt atcctatggt cagcttttta tgtaaaagaa gatactactt
    60301 ctaacacatt gtttatctgt aaaaaagagc gtgggacaaa agtaaagaac acttttgtct
    60361 cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa
    60421 attcacaaaa atttaaaaag caattttctt gagtttcttc ttatttcttg gagttaaacg
    60481 cttctgtccc agcctctttt ttccttcctt caatttttta cacgtcgacg attcaataaa
    60541 aaaccgccta atattgtaaa gacaggtgca agaatcgaga aaaaggcgaa tggcaagtaa
    60601 tgcaacgcgc taactttcat cgttcccata atgaatgtgc cgctgactcc ccacggtacg
    60661 agagaattga cggtcgcacc tgaatccgct agtgttcgtg tcaaataggt ttttgataag
    60721 ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa gaatgatgga cagatactgt
    60781 tctccaacga tcagattgat tccgatacag ctcaaggcag taaacccgac taaacgagaa
    60841 ggtcggtcca tcttttcttt taattcttga acgatggtat caacgatcag atatttgatc
    60901 agtaacccac ctaatgccaa tgcaagtaaa atcaaactag cagaacctaa catactcatg
    60961 atcccgcctc gagaaagcag ttcatctagc tttggatcag gtgtatttgc gacataacca
    61021 ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt tctcatggat aaaagaaaga
    61081 acgagagcag ctgtcgatcc cgctagtaat gtagggatag ccggaatttt tttgattgcc
    61141 cccccaaata aaataactag tggaatcaat gtccaaggag agatccaaaa gctcgctttc
    61201 atgttttgga tcatttcatt gatactagct agatcggctg aatgactgtt atgtcctaat
    61261 atcgtataaa aaatcaacga aaggacgaat gctgggaaat ccgtataacg cataccagcg
    61321 atatgttcga atagattaac tcctccaata cccgctgcca agttggtcgt atctgataaa
    61381 ggcgagcagt tattccctaa aaaggcaccg gaaacgactg cgcctgtagt cattgcatca
    61441 ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc caatcgtcga aatcgttgta
    61501 aatgaactgc cgacagtcac tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag
    61561 aaacgcacag aaacaagttt aaatccataa accataatcg tagggatggt tccagataaa
    61621 atccatgaag caactaagac accaatcaaa agaaaaatca ggattggaat gatccctgga
    61681 gtgattcctt tgatgatccc gtcatggatc tcatcccatg tgaacttttt aaatctgcca
    61741 taaaacaaca aacacatgaa agctgcaagg atcgggatat gtggcgagag ctggaatccg
    61801 atgatcagta ctcctaggag tgccaacaac acgatcaata cagcgatact ttcttttaaa
    61861 gaaatatttt tattcatcgt cgatctccta tttcaaatga aaaggccctg ccatgaaaaa
    61921 actattcact tggcaggacc tcctactaaa tcagcgacaa aacgtcggct gaaagactta
    61981 tttttttatt tttcttctaa acttttttct acctcaatcc cccaagaatc tttcttgaag
    62041 tgattgatga tcaatggaat aacgaacatg accactgtga tagctgctac taataatgca
    62101 tcactcaatg tcgctgtacc taatgtagca aatacgacaa cgatgataga gaagatcagc
    62161 atgcagctga ctacccaagc taatccgttt ccgtttttac caattcggta aggacgttcg
    62221 aattctggat gtttcttacg cattttaagc aatgaaacag caatcaaaat gtacacgata
    62281 caataaataa cagttgtagc atttgtaagt gtcaagaata cagcacttac gtcatccatt
    62341 acaccataaa gcaaagcaaa taatgaaata acgattgatt gtgttagaac gacatttcta
    62401 gatacgccgt atttgttttc tttatggaat ccgaattttg gtggaaggaa tccttcacga
    62461 gctacttgga tgattgtttt tgatggtcca gtaacccaag ctgataattg taaaagcaca
    62521 ccaataaata ccatgaagct gaagatattt ccgatcactt ctggtaagcc taggatttga
    62581 cagtcaatca agataggctg cgtgatattt gctaattcca tttttccatc tggtacgaca
    62641 tttgctacaa gcatcgcatt gatgacattc aataaaacta atccgatcaa agcgataaat
    62701 acgccttttg tataattttt tgttgcgtct ttcaaacgag gcatgtaaac agaactcatt
    62761 tcgatcccaa caaaaataaa tgtgatacca gctaagtatt tcaatgaatc aatatgttcc
    62821 aagtcaggga aagcttttga ccatgaaaag tcacctagat agccgtttga gactagtccg
    62881 actttgaagg ctgccaaaac acctaaaacg aacatgatga ctactgggat atatacccct
    62941 agccatacac cgatgttccc gccgactttt gccatatcga atttcaagtt caagatcgtg
    63001 atgatccagt agataactaa aatacaacct aaaacgaacc agtgattatt gccaagttct
    63061 acattaccaa atgtgtttcc taacaatggt cctaaagtag aagcgaccat taccatacct
    63121 gggaacattt gcacccataa tagccatgca acgacaaatc cccatttgct gcccagtgct
    63181 gtttttaccc ataactgagg accgccttct tgaggaagca tcgtggaaag ctcaccggaa
    63241 accattgata tcggaccggc aaaaaacaaa actgcaaaaa ttgaataaaa aatcaatgtc
    63301 caaccgactg cggctaacgt aggaatactc cgaaccgttg cacacagtgc cattgtcaga
    63361 ccgataaagg tcccaagacc cattttactt acttcagatt tactcaccgt tgacactctc
    63421 cttgattttt tggataaaat aacggtgtct ttgaagtagt ttcagcaata aacgaaacaa
    63481 ttcagcgaat atttgagggg attctttgag tttattgctt gaagggtttc ttcaaagaca
    63541 acctcaaaac aaagggatcg aagagcttag gtctcccctt gctcttcaga tccaaacatg
    63601 cgtcagaaac agttagtatt caagctgttt tctaacatga ttaccttgct tattttgctt
    63661 cgcttgccaa taattggtct gcgtagattt tttccaattt ttcttgaaga gctgctttga
    63721 ttttttcagc atattcttca aagtgttctt ctttgttcat gtatggtgtc attacgctgg
    63781 cacgcaatac agttactttt ccggcacggt tccattcttc atctgagaat cctagttggt
    63841 tcacaaattg taatgggctg ttgccatagt ctgggatagc aaagtcagta tgtgaagtta
    63901 agaactcatt accgtaaatg cttcctttca cgtatgaaga ataatcatat acgtcatggt
    63961 tcaatttatt cattgcaact aagtcatcgt tgcctttttc tttgaataca tagtctacca
    64021 tattgaaatc tggataagtt aatggatgaa cttcgatttc tttgtctcct actttaaatg
    64081 acaagtcatt caagaagttg tagaaacgat gtgcaccttc gattgatgca cccattaatt
    64141 taccatatcc tgtgacattc aaaggtaaga catggtgtgc tgcccaaacg ctggctgctg
    64201 ttgcccctgc ttttgaacct tccaagatat aagccccaag taatgctggg atatctgcgc
    64261 ctttttcaaa tacatatgtc gcaaagtaag agataacgtc tctcatgcgg atatctttga
    64321 tgacgatacc accggcagaa tatggaacat agcccatttt atgtgggtca atcgttacag
    64381 attctgcttc ttcgattgct ttatatgcac tgtgtacttc ttctaagata taatctttgt
    64441 tttctgtaaa gacattgtat ttgtaatgaa catcttttaa atcttcaaat ggaatgaagt
    64501 tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc ataagcagca tctacgtgta
    64561 agtagaagta aataccgtct ttttctaata cgcgacgcaa tgcaacgatt ttatcgatac
    64621 cgtcgattgc tccttcttct gttgatccta caacaccgac tacacctagg attggtgttt
    64681 tttcagctgc tagaccacga acgatttttt caagttcgtt gatgtccatg cgatagttgt
    64741 ggtcaactgg cactggaatc acttggtcta aaccgatacc gatgatgtcg gcagctttta
    64801 gccatgaata gtgtttagtt tgtggaacca accattttcc taatttttct aaatgttttc
    64861 cgctacgtgc tgagtgagct ttgatttcgt cgattttttc tgggacgctg tctaacagat
    64921 tcatgatttc ttctgttgac aagttcatta attcccaatc gctttttcca gcaactagtt
    64981 caggtgtcac ttctttcatt gcaagcggca atgatttgat gttgcgggca taccaaagtc
    65041 cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc ccagccatct ttatagctca
    65101 ttaattttgc aaattccatt cctacttctt cttccatttg gcttgttgca ggagaagatt
    65161 cataggcaac gttattacca ttccatagca tagcaaaatt gtacgctagt aaagatggca
    65221 tcaatgtttc agagttcata tgaccccagt aacgtccggc attatgccaa ggaacggaat
    65281 gggtacgcat ccgcgctgaa atttctgata gaacatcctt tgttttattc acagtgtgtt
    65341 caaaactagc actgcttttt tcttctggcg taataatagg catgtcttga ggcatgtagt
    65401 tttgacgcca gcctaaatgt tcatccacca attcattcaa caaagcttta tagatttggc
    65461 cgttctcagc tttgtcccca atgaataggg catttaggtt tagatctttc gacaatgatt
    65521 cactcatagt gaaaccctcc taaaaaaata tttaaatttt gtcgagccct acattacacc
    65581 tgttcttcta ttctgtaaac taagttttgg cctgattttt aatcatattt taatatgtat
    65641 caattatccg aattgttcac acttttgaaa taattaaaaa aaacgaggga atttacaaat
    65701 gtaaactccc tcgcaaagat tgtattaacg acaaccagcg ttctatttta ggatgggttt
    65761 caaaattgga atgttgtaat agtatttccc tttttcaagg taaaaactta ctaaattcta
    65821 atcaattact ttggccaaaa agtaattttt cttacctttt cgaataacca caaacttacc
    65881 atcgaattca tcggatggat ttatgacaaa atttaaatcg gtaacgcggt caccgttaat
    65941 acttatagct ccgtttgaaa catcttcacg tgcttgacgt ttagatggtt caatttttgt
    66001 ggaaacaagc agttccacga tattttcagg tgtgcttgaa atttcaacgt tcggcatttt
    66061 accgaatcct tgcgcgattt cttccgcatt tagttcttta atatttccag agaacaatgc
    66121 ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta tgcacaaatc ttgtaacttc
    66181 ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct gtctctactt ttttcgctaa
    66241 gtcttcgatt tcttcttgag acaagaaagt aaagaatttc aagtatttca ctacatcgcg
    66301 atcatcttga ttcaaccaga attggtagaa ctcaaatggt gaagttttct tcggatcaag
    66361 ccaaacggca ccgcctgcag tttttccgaa tttcgttccg tcagccttca gcatcaatgg
    66421 gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat tttcggatta ggtctaatcc
    66481 agctgtgata tttccccatt ggtctgctcc accgatttgc aattgcacat cgtatgtttt
    66541 atgcagatgc aaaaagtcga tagattgcag gatttggtaa gtaaactctg taaatgagat
    66601 tcctacttcc aaacggctgg caacgatatc ttttgctaac atcgtgttga tgttgaagtt
    66661 tttaccgtag tctctcaaaa attcaagtaa agagattttt gacaaccagt cgtagttgtt
    66721 cacaaatgtg atatttgcat ctttaccaaa caattttctc atttgattgg aaagtgcgtc
    66781 cacgttatgt tgcacttgtt ccatcgtttg caacacacgt tctgttttgc ggccgcttgg
    66841 gtcgccgatc gttcctgttc cgccgccaat cagaatatat ggatgatggc ctgccaattg
    66901 gaatcttttc atcatcataa atggaatcaa atgtccgata tgcatcgaat caccagttgg
    66961 atcaacgccg caataaagtg aaatgctctt ttcttcaacg agctcgcgaa gtctttcttc
    67021 gtttgtttgc tgattaatgg cgtcgcgcca agttaattca tcaataatat tcattttact
    67081 gacctccgtg tagttttttt atgaaagcag gaacctgcta gcatatgtaa ttaattattt
    67141 cttcaggata ggataggaaa taaaaaaagt cccttggcat ataagccaag ggacgaaatc
    67201 tcgcggtacc acccaatttg tatgtctaga tatccaacat acctctcgaa catttaacgc
    67261 tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa ttcgctttaa agcatgtact
    67321 ggtttgcagc gaccaccagc tttctgaaac attggcttta acaactactt cgtttcgatc
    67381 ttcgtcttgt tattattaat atgtgtctat catagagcgc aactttttct ttgtcaacag
    67441 aaaaaggata ttttttttaa attcctcatt ttttcttttt tgaatccgct ctaattccga
    67501 acattttatt ttcaattatt gttaaaagaa ataacttttt ctgtattttt tctgcaattt
    67561 atcgagaagt cactttactt tgcctcttat tctttcttcg ttacaataag gaatgggaca
    67621 agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa aaaagcaaaa aattatttta
    67681 ggagccactt ctgctttaac tatcgttatt ttaattctaa gtttgttttt tgtcatccga
    67741 tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa taacagaaaa agcaaaagaa
    67801 ccgacagatg ataagcaaca actagttact caagcaaaaa atctggctca aagctacgat
    67861 tatgaccaag cgattgcgtt attggaaaaa gatagtaaga acggatctac tgaaacacaa
    67921 cagcttcttc aaacactaaa aaaggagaaa gaacaactga tcgactggtc tgatcctact
    67981 cagatctctc atgtcttttt ccacagcttg atcgtggacc cgcaaaaagc cttccactct
    68041 caacaggcac aaggatacaa agattatatg gtgactgttg aagaattcaa ccgttcgatc
    68101 gaacaacttt atcaaaatga ttttgtttta gttaatttga atgatttgat ccaaaaagac
    68161 gaacaaggaa atctttcctt taacgggctt gcgctacctg aaggaaaaaa accattggtt
    68221 ctttcacaag atgatgtcag ctattatgaa tatatggata attccggttt tcctagccga
    68281 ttggtcgtat caaataacaa tgaaatcaaa aatgtctata aagagaaaga taaagaagaa
    68341 atcggtgact ttgacatggt tcctctgatc gatgcctttg tcaagaaaca tcctgatttt
    68401 tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt acaacggagt tttaggttat
    68461 cgaacatcaa aatctcaata tggagacaat gaaaaaacaa aaaaagagat taaagaagct
    68521 aaaaaagtgg cggaacagtt gaagaaagac ggctggagct ttgcttccca tacatggggt
    68581 cacttgaata tgactcaagc ttcactagaa gatgtaaaaa aagacaatga attgtggcag
    68641 caagaagttg cacctatcct aggaaaaaca aatattctga tttatccatt tggtgcagac
    68701 atcagcgact ggcagcctta ttcagcagaa aatcaaaaat ttgcttactt gaagagtcaa
    68761 ggtttcgata ttttctgtaa tgtcgatgct tctacaccag cttggggaca attaggtacg
    68821 gatttctatc gaaatgctcg gatcaatatc gatggcattc gttttcaatc tgatttagac
    68881 ggaaaaaatc ctattcttga tccattcatc aatgtccatg atgtctatga caaagaagca
    68941 cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa agccgaactg acattttatc
    69001 gtcagtttcg gcttttttat gctttatttc ttcttcggtg cgattccctt gatcaggact
    69061 tctgtccatt ctgctgtata tggaagcagt tttgcgtata tctccggctt agaaattcct
    69121 tctaccattg cttgaaaata gaggatcaag aactcatccg aatatttcaa atccactttt
    69181 ccttctttcc gccctcgatg gaaaagatca agcatgatac tgaaactttc ttgcgtatac
    69241 gcatccatcg cgtcagaaaa ttcttttttt tctttttttg tatagtatgt catcaaatcg
    69301 atataaaatt gtttgttcac tcgattgaga taacggattt tgttttcact catggcaatc
    69361 agtgtttctt caaatggaag attttccgca atcaacgctt tcgcttcttg ccctaaatca
    69421 ttcataaatg caaaaaatac ttcataaatc agcccttcct tgctgccaaa atatttaaaa
    69481 atcgtcgctt tacttacctg agcgttctca gctaccttat ccattgtcac tccagcggtt
    69541 cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa tctctttctt tttctcttgt
    69601 gctctctttt caaaaccgtt catttgtaga gcctcccgta attttaaact tttataatat
    69661 atatagttca aatttattct gaggtttatt ataactatct attgagaaag tatcaagcta
    69721 gcaattcctt gtttatgaat tcattgacag aaatttataa aaagaatatt atgtactaaa
    69781 ataataaaaa aagttttata aaaaggtata aaaccttttt attaaactat ttaatcttgt
    69841 ttagtatata tttttagaaa aaggagtggg gtttttatgg cagaaatcgt caaagtcaca
    69901 catttgaaaa agagctttgg tcgtttcgaa gctttaaaag atgtctcttt caccgtacat
    69961 tcaggtgaag tcgttggatt tatcggacca aatggttctg gaaaatccac gacgattcgg
    70021 acacttttag ggattataaa aaaagacggc ggaacggcaa cgatttttgg gcaagatgta
    70081 tgggaagata gtttatctat ccatgaacgt ctttcttatg ttccaggcga tatctctttg
    70141 tggggaaatt tgactggagg agcgatcatc gatctcttca tgaagcttca tggaaaaggg
    70201 agtgctcaaa aaaaagatga actgattcaa cgtttctctc ttgatcctaa gaaaaaagca
    70261 aaaaactatt caaaaggaaa tcgccaaaaa gtgggcttga ttgctgcttt ggctgttgat
    70321 tcggatctct atttatttga tgagcctact tctggtttag atcctttgat ggaaacgatt
    70381 ttccaagaag aagttgaaaa attgaaagaa cagggcaaat ccatcctact ttcttctcat
    70441 attctcagtg aagtcgagcg actagcggat aaagtcgtga tcatacgcga aggaacactc
    70501 gatgaagaag gaacactcga tgaattgcgt catttgactc gttcgaccat cactatagaa
    70561 acagaagcag agaatagtca cttagcacaa ttgaacggtg tccatgactt tgtgcaaaaa
    70621 ggtcacctcg ctcaatttgc tgctgataac gaagctttcg atgtgatttt agctgaagcg
    70681 tccaaattag gtgtgaaaaa atttgaatct attccaccga ctcttgagga tttattcatg
    70741 agacattatg aaggatgagg aggaagcgaa atgactgata aattttcacg ctggtccacc
    70801 cttttttctc aaaaccttag ccgtgactgg aaaaagatcc tgatatggct agtcggtgtc
    70861 actgcttttt ctggtggttt tgtccctgcg tttgaagaat tgacttccgg acaaggatta
    70921 gctggattat acgaaacctt gcaaaacccg gcaatgattg caatggtcgg accaactcca
    70981 gcttcgaatg ctgctgagta tactttagga gcgatgtatg caaatgaaat gttgcttttt
    71041 tgcagtttgt ttgctgctgt gatctctgtt ttacacgtgg tgagccaaac gagaaaaaaa
    71101 gaagaacttg gtctgattga acttattcgt tcctttcgag tcggacgtca agcaaactct
    71161 ttagctgtca tcgctgaaag tattctgatc aatttgcttt tagcaatatc gatcagctgt
    71221 acgatgattt ttttccaagc ggattcgatc actgcagaag gtgcttggct ttttgggcta
    71281 tctgtaggta gtatggggat tttggggagt gccgttgcac tgtttgctgc acaactgatg
    71341 ccaagttcag ccagttcgaa tggtctgtct ttagcgtttt taggtggact ttaccttcta
    71401 cgagcaataa cagatgtagc tgattcctcc ctttcgatgt ggaatccttt aggctggtct
    71461 tatttgacct atccatttac aactaacaac tggattcctt tgctttattt gtttttgttc
    71521 agtctaatct tgataatcag ttcatttgtt ttagaggaac atcgagattt gaacaccggc
    71581 tatatccccg aacgtgaagg aaaaccgtat gctaaacgtt ctcttttatc cttgcgtggt
    71641 ttattattct atctaaataa agggatgaca atcagttggc ttatcacttt tggtttgatg
    71701 ggggcggcat acggctctat ctacgggaat atgcaacagt ttttagaaag taacgactta
    71761 atgaaacaga tgtttatcca atctggcgtc tcaattgaaa aaaactttac aagcacgatc
    71821 atggtagtct tgatttgtct agctgctatt ttaccaattg ctagcatcaa tcgtttattt
    71881 acagaagaaa cccacggacg tatgaatcag ctgttcgcaa caaaaatttc taggaaccaa
    71941 ctttattgga ccaatatgtt actagctatt ctatttgcta tattggcaat cctgattagt
    72001 tctggtgcac tagggctttc tgctttggct tcgatgaagg atgcgaaaga catggatcta
    72061 ttgatgtttc ttaaagccgg gttcaatttc ctgcctgccg ttttagtgtt tatcggaatt
    72121 agtggctttc tattcggatg gcttcctcga atcggtaaac ttgcttatat ctacttagct
    72181 tactcctttg cactcaatta ctttggcgga attttggatt taccagaatg gttttccaaa
    72241 acagctgtcc ctagctgggt tccacggatg ccaaacgatt cttttgatct ttccgtgttc
    72301 cttacgttat catgtatcag ccttgcactg ttcgtccttg gttctattgg ttataggcga
    72361 agagacttac tagaaggata ataaaaaagc atgtacaatt tcatgctagc ctccaaatct
    72421 gggtcaaaaa tccaacattt ggaggtcatt tttttattta caagtcttct aactgctctt
    72481 tatcaatgat ccagacttct cgattctttc tttttacata accattctcc tctaataatt
    72541 taaattttct agataaagtt tcgggggtcg taccgataaa tgcagcaagt tctttcattt
    72601 tcattggcag gaaaaagtga tccttccctg tcgctttagc taagtcaagc agatagacag
    72661 cgactcgttc ttctactttt tccatcatca aaaaagttgt ctgctgctgt acttttgtta
    72721 atttctcaac gttgattgct aaaagtttca gactaagggc gggataatgc agtaagagtt
    72781 ccgagaaatc tttttgtctc aatatgcaaa tttccgtttt ttctagtgct tcgccaaata
    72841 aagactcatt tggaacgcct agcaactggc tctctccctc atatccgccc ggctctatta
    72901 ctcgtaaaag ctgttccttt ccgctagcag ataattgata cactttcata ctgccggagg
    72961 ccacgatcac taattgaggt tctccattcg gcgcaagaat ctgttctcct ttttccacat
    73021 agtgatgttt gacaagtgcg tgtacttttt cttgatcttg ctgtactaag tgattgaaca
    73081 aaggaacaaa tgaaacacat aaatgatcat gactcataat ctctcctcct tctaaacagc
    73141 tatttctaat aaagaaacat aacttccctt attccaactg ataattttct tttatcatag
    73201 cataaacgtc aagcgaacca gataccagca aatagagtgg atttcttttt gtcccttgct
    73261 ttattttcct tcttttgaga ccttaagtaa ttgattccta tcaatttttt aaatatttac
    73321 tttttgaata atcaaaaaca aaccttcact cgcaagccga gagtaaggtt tgtttttcgc
    73381 attgcttatt tcgtgttgtc tattgaaatt tttccgcacc tagcttttga tatagtggtg
    73441 ctagttcttg taaggtttca gcggttattt gctcaacgtg ttcgatttct tgcggataga
    73501 agaatagttt acccatttga aaggttcggt ctctcatcgc ttttttcaac tcttcttgtt
    73561 tctttgtatc ggacaaatag tcagacaaag ctaagtggtc gtcaagttca tgttctggtt
    73621 gcggccatat gtaataatcc ttgatatcga ctgttttgct ccactctttc agtatcgcaa
    73681 gcaactggtt atacgctgca atgctaccat atcgttcttc gcttttataa tgttggtaca
    73741 tcaaatagat ttggtattgt ttttgattca acaaaactgc tagacaggca ttctcgtctt
    73801 gacgagattc gcttcggtac gcgctccaaa aatgactgcg taaattccag ccattcgtcc
    73861 agctttcgat tttaggtttg ctcatgccgt acatatcagg caaaagtgca gctgtctgga
    73921 gctgaatctc tttccatttt tgccagtgtt ctttatattc tttcttgatt tgttcaatct
    73981 tatctgttgg atggctctct ttgagctgtt tgaatgcgta gattggtcga tcaaacaact
    74041 gataatctga ttttccaagt tccattgttt cgcctctttc cgttatcttt tgtattctac
    74101 ctgaattttg cgatatggac aaatagaggt tgtgaaaaag ctgttctgca tcaagtcata
    74161 agaacagcca aacaaaaaac tgcgatgagc atcaccgcag ttttattgaa cttaaggttg
    74221 attgatcaca tctaaaagcg ttgaaaataa aatggcacta gaagcagctc ctgggtcgat
    74281 atgtccgatt gcacgctctc ctagatagga agctcgtcct tttttagcta ccaagacttt
    74341 ggtgtgttct tttgcctggt caatgatttc ttttgttaaa ttccctgcct tcaatgcctc
    74401 tgtgactggc agccaaacat ctaacatcgt tttatcgtca gcttctgcct ttcctcggct
    74461 ttgaataccc gctgttccct gctcgatcac ttcgcctaat ttttcttgag aatcaataga
    74521 atcgacctct tttgtcgctt tcatcatgtt catgaatgca gatccgtata acggaccaga
    74581 agccccaccg actttcgata tcatcgccat agagaatact ttgaatgtat cgctgattgt
    74641 ttcaggatga tcggcttgaa aagcttcttc ataagctgaa acccctcttc ccatgttatt
    74701 tccatggtct ccatcgccaa tcggggtatc tagatcactg agatattgtt tgttctgatt
    74761 gattgcctct gcaaattgcg tgagccattc ttggatttct tgaacggtta gtttcatatt
    74821 atcacccttt tatcttgaga ttttttacca tgaaatcgtc tgtacattgc tttctaaaga
    74881 atctttccat tgtgtctccg tcaagtcgat cagtgttaga gaaagacctt gcatatctag
    74941 tgaggtcata aaattaccga cttttcggaa aactacattg acgcctcttt cttccaacag
    75001 agctaaaaca tcattcatga acacgaactg ttccatcaaa ggcgtcccgc ccattccgtt
    75061 gactagtaca cctgcttctt ttggtaaatt cgagtaatca tctaaaatct tagtaacaag
    75121 ttcctttgcc agtactttag aaggctgcat cttttctcga cgatagcctg gctcaccgtg
    75181 gataccaaca ccatactcga tctcatcttc tcctagttcg aatcctggtt tccctacttc
    75241 tggtacagtt gctgctcgaa gcgctagacc gatcgtctta gttgcctgta cgaccttttc
    75301 tcccagttct ttcaactcag ctagggaagc accgtttctt gctgcatcgc ccacgatttt
    75361 gtgtacaagg acagtacctg caactccgcg ttttccagca gtatacgtac tattttcaac
    75421 ggcgatatca tcatctacga tcacactttc tacttcaata tcgtccatag cagctaattc
    75481 ctttgccata tcgaagttca atgcatcccc tgtataattc ttaacgatca gcaaaactcc
    75541 ttttccacta tcggcttcct taatagccgt ctgaatctga tctggagttg gagaagtaaa
    75601 cacatcacca agcaccgctg cacttaacat cccttcccct acaaacccgg catgggatgg
    75661 ttcatgtccg cttcctccac cagaaaccaa accaacttgt tccgctttct gattcttggc
    75721 tacaacacgg gaagattcta cacgatgaac aagttctgga tagcttttta ctaatccttc
    75781 cagcatttct tcgacaatat tgcttggatc attcatgatt tttttcaatt tattcccatc
    75841 ctttctttcc ggcattttgt gcttctgtta agatttgctg agtatcatct gttataccgg
    75901 cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc atccaccaga tgataacgtt
    75961 gctgctgctc ttcttccaac atgtcttttg ccagttccga gttcaatacc gcgctaccga
    76021 tatccgcaaa aacaaaataa ctagctgcat cgcttgattc attgactgct tctacgattt
    76081 tcatcgggtc tgatccgatc tcgccattta ttccgccgag agaaaaaatt tgcacctctt
    76141 cggatttttg catctgctca atcatttctt taataccatc tgttactttt tgactatgag
    76201 aaataagcaa aatacttttt ttcacaagaa tcttcctttc ttttacatag ttgctaacga
    76261 ttggtttcgc tttcattaat gaacaattca agtatataat aaatttctaa ttttgggaaa
    76321 atatgtgtag tgcttcttga agtttgttat actttaatta aaagagagga gtgatcgaat
    76381 gattacaaca acaacgaaca gtgtagaggg aaaagccgta gtagcttata aagggatcgt
    76441 ttttggtgag gtcattactg ggatccatgc atttaaagac ttggaggcgg ggttgcgaaa
    76501 tatctttggc ggccgctcaa aaagctatga aaacgaacta atgggtgcaa gagaagaagc
    76561 acttgccgaa atggccgaac gtgcaaaagc attaggagct gatgcgatca ttggcgtaaa
    76621 aatggactat gaagtattag gcagcgacaa tggcatgctg atggtcacat gtagtggaac
    76681 agctgtctcc acgagataag taggggacat ctgttttaat aactaaagag agatgggaca
    76741 tgtagtggca ctcaagaatt agatttttca gtctaacttc gaggtactct accttcccat
    76801 cctctctttt ttatttcttt ttgcggatga ttccgtaagc tgctgcgact aacacacccg
    76861 cccctgctaa ggaataactg acagaagctg ttttttcatt tgtttttggc aaagtctgac
    76921 ttgttgattt ggatggctct gtcgtttgct gaactgtttc tgtcgtgctt ccagaggtca
    76981 ctgatttttc tcctgccgaa tgcaaaatat cttttattcg ttggaaacta gctgctaagt
    77041 cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc ttcgctgatc tttccgtgga
    77101 ttgcttgact ggccgctgtg atatccgctt gagtttgact tagctttcca gaagctgttt
    77161 gagcaacctg atttaattgc tgattgaatt cttttgcttg ttggatcttc ccgtcaagat
    77221 ctgtttggac agcttccgct tttgctttcg cttcggcaag ctttttctca agttgtgcac
    77281 ggtcatttcc cactttattt ttaagatcag ctaaatcttg atcgattgct gcttggtctt
    77341 ctttcaattt gttttttgca gcttctaatg cttcttcgag ttcttttctt ttattttcca
    77401 agtcttgcaa atgatcgatt tggtcagaag aggtagaact atttattgat gtatcttctg
    77461 aagatgaaat cgtttctcct gaattatctg tcggtacatt ggaagtcatt tctgtttccg
    77521 aggcaccgtt ttcagcacca gaagagctta tggttgttga aggttctgtt tgagcaggtt
    77581 cagaagtttc cgcaaaagct gtctgtccca ataagaaaat actggaaaca gcaattggcc
    77641 agattaacgg cttcattctt tttttcatca cttttcccat ccttactact ttttaactaa
    77701 tttcttatta taagcaaaaa tgtttggaag aaccataaac acaacaggct gtttgtctaa
    77761 aaaacggtgc atcttttatt tttttgtacg ttccgggctt ttccatgtcc cgcttccata
    77821 acctaaaatc gttttgactg ctggaatgaa agtcgtcacg atcgttatcg cgttcactat
    77881 ccagaacaaa agcatatata gtggtgcgaa aagaaggtat ttcaattttc ttcgtcgatc
    77941 atctacaatc agcgaagcta gcaactggaa aaatcctgcg accatttcaa agcagatgaa
    78001 cacaaaactg aatgtaaaag taatataaat ctgttcgtag tttccttgat atacataatg
    78061 cccgattaat ccagcaaaaa gaactacgga aatacaaaag aatattgacc agacaatact
    78121 caatgtttga tcaatgaaga tgatcgttct tccaatatgt ttaaaaggat ggagcatcac
    78181 ttttttgaaa ttcgtcagcc agacttctgt tccacctttt gcccaacgtt ttctgtgtcg
    78241 atatagcatc ttcaacgtca caggtacttc catgaaaaag ataatctcag gagcaaaaac
    78301 agataaccag tcatttaact gatgatccca agcaatactg atgtcttctg tcgctcgatc
    78361 ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga tacatcgtat ttgctccact
    78421 gtacgcataa agaccaccga aaacagctga ctgtgttcgt ttaatgatgc ccacgatact
    78481 tgagaattca accgtttgag attttgcaat caatttggta cgattttgga catccatatt
    78541 tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg aaataattca catacctaat
    78601 cagtgcatct ggctccggta ccgtgtctgc atcattgctt aaaatcaatt tcccttttgc
    78661 aaaggcaagt ccgatattaa acgcgtgggc ttttccttta tttttttcga tccgaaccac
    78721 acgaagatta gcgtattttt tcatcagtct agctaatatt tctggtgttt ggtccgttga
    78781 accatcgtct gtcactaata cctcatagtt gtgataattc aatttcgtca tcaaatactc
    78841 gattgtatat tcgatcacaa tttcttcatt atgggctggc accatgatcg tgatgaacgg
    78901 ttcgacactc aagggaacat ctacccattc tttttgctta tgtttgtata aaaacacata
    78961 acaccagaca ccaataaacc atacaaatcc gcctaaaatc ggataagaaa cgagaaaaaa
    79021 atacataata gacaaacaag tatctaagaa taaattacct gtatgggtga ttgagatcat
    79081 ttcagttcca ccccattttc tttatacaaa tctgcaatga atgtcgtatc tagatttttc
    79141 tcttcggaga cagaataaaa acggaccgtt tctctctctt cttttgttcc aaaacgttcc
    79201 gtatacactt cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc atattcaaaa
    79261 gatttattta cttttttgta tagatttcga ttgttccacc tagtcaatac aaatgaggta
    79321 atcaggaaac tgaaaaaaat caatacaaaa aagccgatta agaaatacag cattttcttt
    79381 tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa
    79441 aagacccata taaaaggaag aacaacgatc atccatccta aaagagccac taacgtttgc
    79501 caaatcttta accagtaatt accttttgaa aaatacttat ctttcaccaa aattgatgca
    79561 acttcttctc ttgttacttt tttcatatcc tcacctcacg tttttttgtt acgacgtagg
    79621 agtttaccat acatttcatt tcttttcaca taaagtgttc ctaaattttt cctaagaaaa
    79681 agaacaaaac atttctcatc agcaaatgaa aaatattttg ttctttttat cagttgttct
    79741 ttttattttt tatctcttaa ttatgttgct ctttccaata cgacaatgct tttcattcct
    79801 ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct catctccatc gttttcaaat
    79861 ggatataaga ttacatgatc aaattccagt ccttttgcta gactcaaagg aatgatttgg
    79921 cagttttcta atccatatga atttttttta atcgattctt gtagcgtttg ggtttcctct
    79981 tctgtttggg tgataactgc cgctttttct ccttttagag aaaccaaaat ctcagctagt
    80041 attttcaaca attcttcaga ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt
    80101 cggatcgata cgatgtcgat cttctcttga tccacagcat aggttttgaa aagctctgtg
    80161 attgctccgc tagaacggta actatttcta agatcatacc gatggatcgg cagatggtag
    80221 tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag aattaaagat tgcctgattc
    80281 tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag acctaattgt
    80341 gccagggtat aatcttgtac ttcatcaatc aatagatagc gaaatgctgg tacatttacc
    80401 ttttcaacaa gtaaatgacg aatagctaat ataatgactg cttcatcaac agtacgtgtg
    80461 ggttgccaag cataagctga gccatatcgt ttttcataaa ttctttcaaa caactgatgc
    80521 tcatccaccc atgccatttc ttcgatttgt ctggttattt ttcgatattt tttttgtaaa
    80581 agtttccttg cataagctac aatactttgt tccgaatcat cagaaatcaa tttaccgaat
    80641 aattccaact gcctatcttc tgtcaacgag agtacctgat tctgcacagc aggacttttt
    80701 gcttgtttca aaagatgatt cttccattcg ctcatcagtg cctttttcgt tgcttgaagc
    80761 ctatcgatca atttaggatg agcaggaact ttctcatata ttttttcgat cgttttttta
    80821 gaaaggactg tttttccatt tttagtaagt ccacggataa aattcggatg atctaaaaac
    80881 aaaggatctg attgttttag attatcgata aactttttgc tacgcaaacg ttctgtctgc
    80941 tccgatacat tttctcccgt gatacgtttg aaataagcct cttcaccttc tatttcctct
    81001 gctgaaagct gtgaaaccag ctgtatgatg gttagattca gtggatttct ttcacctaat
    81061 gatggtagga cattcgaaat atagtcgata aaccgatgat taggtgatag aatcagcaag
    81121 tcatcggatg tcatcgtatc tcgatataaa tagagcagat acgcaatacg ctgcatgata
    81181 gcagaggttt taccacttcc tgctacgcca tttaccaaga tattttgact tttcacatcc
    81241 cgaatgattg tgttttgttc cgattggatc gtagcagtga tagccttcat ttcattggtc
    81301 tcatcttggc ctaagacttc taataatacg tcatcttgaa tagctacaga cgtatcaaaa
    81361 aacttgacca aacgatctct ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg
    81421 attttatttt gacgaaccgt ataagaagat ggaccaatca tgttattgta aaaaagttcg
    81481 gcaattggtg aacgccaatc ataaacaaga ttttcatctg catcatctgt gaaatttgct
    81541 gtaccgatat aaaaggattc ttttggctca ttatccagaa agtctacaac gacttttcca
    81601 aagtagggac ttttcaataa acgttcgatc tttgctagct gattttctgc gtttttctgt
    81661 ttgatattca tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa agtatccagg
    81721 ttatctaagt aactgtcaaa attcaatcga agctcgccag ccatttcttt caacgactgg
    81781 tttccttcta tttttgccgt atccatcgat tcccgcagtt cgtttttttt atcaattagc
    81841 ttttgataca cgttcttcag atatgcttct tctaactgtt tttgtgtgtt gaccatacac
    81901 attctcctct ttttttaaag tggtggaacc gattagttta cctcttttaa gagtcgaatg
    81961 caagcttttt aacgagtatc atttgttagg atatatcagt tcttcaggtc ggacagtttg
    82021 atataaagaa aatgcaccat caaggttttt tacgtggtag ccagactgct tcaagattct
    82081 ttccgctaga tagctacgta atccgctatg acagctgacg atatattctt gttggctgtc
    82141 taattcattt aatcggtccc gaagttcatt caaagggata tgtttggctt ccttgaattt
    82201 gcccttttcc aattcttctg ctgttcgcac atctaataaa atttttcctt ttgctagttc
    82261 tgtcggcaat tcatgccatt gtacggtttc actaagccct tctgcaatgt tcattgctgc
    82321 atatcctagc atgtttactg gatctttggc tgagccaaat ggcggtgcat acgtcaattc
    82381 taattccggt aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat
    82441 ccgtttatcc acaccttttg ccccaacacc ttgtgcacca taaatctcgc cagtctctgg
    82501 atggaagatc aacttcagta aaatatctgt tgcttctgga taatagcttg catgatcttt
    82561 tcctgataca tgaatgactt caaaagacag ttcgttttct ttggctgttc tttcacttaa
    82621 accggtggat gcggcagtca ttccaaacgt tcgtataatt gctgttccaa taccgccttg
    82681 atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg
    82741 tgaagcaaga gaaataagtg catcttttcc agtgatttgc tgttttacaa cgatagcatc
    82801 ccccaccgca taaatatcag gaatattcgt ttgataacgt tcgtctacta ggataccccc
    82861 gcgtatgcct aattcgattc cagcggtcac tgctagctgg ttatcaggac gaacaccaat
    82921 ggataaaata actaaatcag cagggatggt acgcccatca tccagtcgca atcgtttccc
    82981 gtggtcttca aatccagcga cagcatgaga agtaatcact tctactcctt gatgagacaa
    83041 ttctttttct ataaaagctg ccatttcttc atccagtgtt ggaagaatat gtggtgcttg
    83101 ttcgatcacc atgacttcta aacctcttct ttttaggttt tctgccattt ccaatccgat
    83161 gaatcctgca ccaacgatca cggcacgctt tggctgtttt tctaatgcat gcatcacttt
    83221 atcgatatct ggtacatttc taatagaaaa aacattgtcg gcttctgtta tccctggaat
    83281 cgatggaaca actggttttg caccaggaga taaaaccaat gcatcgtact gttcaatgtg
    83341 tttttgaccc tcatgtacga cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc
    83401 atgatttgga cgcacatcta aacaaaaacg agcggataat gattctggcg tttggacaag
    83461 aagattttcc cgttcactga tttctcccga aagataataa ggcaagccgc aatttgcaaa
    83521 agaaacatac ggtccttttt caaaaacaac gatttctgca tcttccatta atcggcgaag
    83581 ccgtgtagca gcagacattc cacctgctac acctccgaca ataacgattt tcatcttatt
    83641 tccctcctac tgttcgatca aaccattgat tcatgccacc acgtacattc actacatcat
    83701 aaccagattt ctttaattct ttagctgctt gtttactcct catccctgat tgacaaatca
    83761 catagacggt cttttctttg tctccatcat atctgtttat actttgtaat ggaacatttt
    83821 tagctccttt tatatgcccg ccccggtatt ctgcaggtgt tctaacatca agtaacgctg
    83881 gattttccaa aagcttttcc tttaattctg ggactgttat cgattttatt gattgaaata
    83941 gcaaattcat tttctgcacc tcatttacat ttttataaat acctcatggg gtatattaaa
    84001 caggagcttt attaatatgt caaccagaat gactcttcat ttttttcact actttttata
    84061 tttgcataaa ataccgataa ggggtattat ctaattataa aaatagagga gaatgtgtta
    84121 tggagattga taataaaaat atcctgaacc gtttgaaacg tacagaggga caaattcgtg
    84181 gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact caattaagcg
    84241 ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt
    84301 gctttgagaa acctgatagt aatccagaag aacaagcggc taaactagaa caagcgatca
    84361 agatgattat taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca tattgatgaa
    84421 ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa
    84481 acgacgattg tcgtatctgt aaatggcatc gcgattaccg gttatctaga ctctgccaat
    84541 gcagaattct tagatttgca aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct
    84601 aatcatattt cagaaagttc tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt
    84661 gatttagttg aactgcaatc ccaaggttat gcttatatca agccttagtc agtagaggtt
    84721 gcgacagaag tgatcagctt caagaaattt cggcttattt ttcgaagaag ctacttctgg
    84781 aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa
    84841 acaaaaatag cttctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    84901 tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa
    84961 aaacgggaaa atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg
    85021 attgaacacc gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa
    85081 aattttgtga aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg
    85141 gcttttgaat accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga
    85201 gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc
    85261 agctgattga acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa
    85321 gaaataattt caaaaacaac ttctcctgtt tttggttcgg ttggcttatt atcgagaagt
    85381 agctactggg agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta
    85441 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
    85501 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
    85561 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
    85621 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
    85681 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
    85741 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
    85801 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
    85861 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
    85921 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
    85981 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
    86041 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagaca ctgatattat
    86101 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
    86161 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
    86221 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
    86281 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
    86341 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
    86401 tattcattga caaatatccg atcacctttt gcaaagagct gacgttcttt tttattctcc
    86461 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
    86521 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
    86581 atccacaaag cttggctttc tcctgtttct tccaacccgc aactgatatt taggacgaag
    86641 tgcttcccga tcgaaagcca tagcagccgg cacaaaatct tcatgatctc cggcgaattt
    86701 cttgatttct ccataatggg tcgtggcaat gatcaatgcc ccttgctcat acatagattc
    86761 catgattgcg atcgctagtg cagccccttc atttggctct gttccactgc caatctcatc
    86821 taaaagtacc aacgtattcc tttttatctt tttcaaaata gcagcgatgt tttgcatatg
    86881 accagaaaat gtactcaacg cattttctaa attttggtgg tcaccgacat cgacgaaaat
    86941 ctcatcaaag acagcaagct ctgtcccttc ttttgccgga acttgcatac cgaacatcgc
    87001 catcaaagtc aataaaccga ctgtcttcaa cacgactgtt ttgcctccgg catttgctcc
    87061 ggtgataatc aagcctcgat agtctttacc gatttcaaca tccaatggta ccgcatgctc
    87121 aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt tccgatttat tgattctagg
    87181 tgtgcttcca ttgatttctc ggctaaattt tgcccgagca aagatgatat ctaacaccgt
    87241 gatcgtttcg atcaataaat cgatcacttc ttcgttttct gcgatagcac cagtcaatgc
    87301 cgccaggact tgatattctt cactgatttc ttcagctttt aacaactgat agtgttcatt
    87361 caatttagaa atcacagttg gttcaataaa aaccgtcgtt cttttatttg attcatcgat
    87421 aatcgttcca tctattttat ttttatagct ggctttgata ggaatcgtat agtattcgcc
    87481 tttttgaaca atcattgctt cttggatcat ttctttgttt ttcggatgac gcaagaattt
    87541 cagtaatttt gactggattt ctttttcgat tagttgtaat tgtttgcgta ctttacgcaa
    87601 attacgtgac gcgtcgtcac tgactttatt attttttatt ttctggtcaa tcaactcttc
    87661 tacatttatc aaatcaggaa gatgtttgct ataagcaaac aacaagggtg tctggtattg
    87721 attcttatca aaaaactttg tgatcatccg actgcttcta agaaaatccg catattcgat
    87781 caaatcggtt ggctgcagaa tcaacccttt tttgacttgt tctgtcagtg catcgatcct
    87841 cggcaacccc ataaatggaa catgctgact gctttctaag atcaagcgtg cttctcttgt
    87901 ttcttcttga cgatccaaga cagtttgtag gtttgtcgag accgtcatat cgctgattcg
    87961 ttttttgcta tagtttccaa ttgctcttgc ttggatttcc tttttgatcc gatcaaattg
    88021 taattgacta atcatttcgt tgtccatatt ctctcctttt cttgaagaga tacctgtccc
    88081 aaaataaata agggcctaga gataaataaa aggatagaac acagcttaaa aaagtcatgt
    88141 cctatcctta ttagaataca aagatattgt tttcctgaca ggtaccgttt cataagcaca
    88201 ccaaataaac tttgtttatt ggctattcga cttactgatt agataccagc tacactcaca
    88261 aaacataccc cattcttcta ggtcttattt attttccact taactatagc aagacacatt
    88321 ttggttgtca aactttagta tgttttatag atttacacca ttaatgaata gacacctacc
    88381 gcgataattg ctccgatcat tggacctgcg attggtaccc atgaatatgc ccaatctgaa
    88441 gttcctttat tagctattgg caataccgca tgtgctaatc gaggaccaaa gtcccgagca
    88501 gggttgattg catatcctgt tggccctcct aatgaaagac cgatggctaa gatcaatcca
    88561 cctacagcga atacgttcgt tccaggtgca aattcgtttt gtccaaaagc tagcaaccca
    88621 agaactaata caaatgtacc aattaattct gtgatcacat ttgctggata attccgtata
    88681 gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat ctttcgttgc attccaatgt
    88741 ggcaaataag aaagccaaac aagaatggcc cctgcaaaag ctcctaaaac ttgcgcaatg
    88801 atgaaaggaa cgacaagatt ccaactaatt gcccctgtca tcgccatagc tagagagact
    88861 gcaggattca aatgagctgg gcccatgaat ccagaaatat agacagccat tgttacagcg
    88921 agtccccaac caaatgcgat gacaatccag ccagaagcct gtgctttact tttgtttaaa
    88981 ttcacagccg cacacacacc atctccaagc aaaactagga tcagtgtccc aataaactcc
    89041 cccatgattt gagtcatatc actgttcatt tatttctccc cctttagttc ccgcagatct
    89101 gattctgcaa tcaatgcttc taaacgttta gtctgttgcg ccttctcctc ttctgtccat
    89161 tcaaaatatc cagcaattgc atcgatcaca ggttgcttga tctcatcaag ctgatctcgt
    89221 tcaaacaaca gatgatttgt acgacgaatg agataatcgc ctggggccaa aaccatttct
    89281 tcttctaaac cataacgtaa ccgagctgac tcagcgagac tcaaaccagg ataaggtgcc
    89341 atttcttttg ctaattcaaa gatacgtcgg gcattagtcc cgtaaaaatc agcgatataa
    89401 gtagcatctt cttcctctaa accggctgct actccttctt ttgctaattc tgtcactgtt
    89461 tcttctaatt tagttggatc gaagtttcca cctgaaatct gatatttttt agagtcgatt
    89521 tctttcgtct ctattccgta ttcttctttt aataattggc gtatcaatcg caaagctcct
    89581 tctgccatct tacggtaatc ggtgattttc ccaccagata aagtcagcaa accatctggt
    89641 tctctttcta aagaactacc tctggaaatc gtagaaggtg ctttttcatc acggctgttt
    89701 tccaaatggt tcaacacgtc ttctacttca gcacgagaaa ctttgttttc cttatattca
    89761 cttactgtat cgacaacttt attgaagctc ttgtctgaaa tcgatccatt atctccacca
    89821 ttgtaatcag aaccagaatt accaatcaac agtggacgaa gtcctgccca gcttgcttcg
    89881 atatcattca atgtgatatt tgcttctgga tagcgatgat tgatcacatc caatagataa
    89941 tctacatctt cttgtgtgac tttcggatcc gtaaaatctc cttggtaatc cgtatctgtc
    90001 gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa ccatccgccc atcttgcttt
    90061 cctgtatcga agtatgtggg ttgcggtaca ggcagttttt tcgcatctac aactaaatgg
    90121 atccctttag ttggacgcat tttaggagag actggacgcg taaaattcaa gtttcgtact
    90181 ttatctaccc aaggaccact cgtattgatc actaattttg ctttgatttc gatcacttca
    90241 tctgtcagca ggtcacgagc tttgacgcca acgatttgat cgccatcata taaaaagcca
    90301 accgctttca ttttacttac tagataagcc ccatcttcgg cagccttttt gatattatca
    90361 atcactaaac gggcatcatt gttgcggaaa tctagataga cacccgcgcc ttttagccct
    90421 tcttttttca aaaacggttc tctttccaat acttcttctg gtgtcagcgt ataattctca
    90481 tatttggttc ctgtcacatt tgccagtttg tcataaaggt ccattgctac tttgaccgag
    90541 aacatattga aggttgttgc tccttcgccc tcatagattg gcaaaagcat tggatctggt
    90601 tttgggatgt gtggggcaat actttgtacg actgcacgtt caccaactgt gtcagctact
    90661 acttccacat caaatgtctt cagataacga ataccgccat gaacaagttt ggtcgagcga
    90721 gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc ctgttttgat tcctgatgct
    90781 gctgactgga ctgccacacc ggcaccagtg attccgccac cgatgatcaa cagatccaat
    90841 tcttcttgct gcattttttg aatgctatct tgtcttgtct tgtttgaaaa catatggatt
    90901 tcctccttat tcaaatgctt gggtagcttt tactgctttt ttccaaccgt tgtaaagttt
    90961 ttctcggcgc tcttcctcca tttgaacttc aaatagcttg ccagcttcat aaaattcgcg
    91021 gatctcgtct gtatctttcc aataaccgac agctaatcct gctaaaaatg ctgcccctag
    91081 cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca ttcaagatat ctgtttggaa
    91141 ttgcatcaga tattcattgt ttgctgcacc gccatctact ttcaatacag gaatcttgat
    91201 tccagtgtca tcttgcatcg tatcgatgat atctctcact tgataagcaa tcgactgtaa
    91261 agtagcttta atgatatctt cttttgtcgt accacgtgtc aagccaaaca tagcccctcg
    91321 tgctgcctga tcccaataag gagcacctag cccaacgaat gcaggaacaa cataaacctc
    91381 atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg ctagcttttt gcaacatttg
    91441 aagtccatct ctcagccatt gaatcgaaga accagccaca aaaatacttc cttctaaagc
    91501 atagtaaacc tttccattta tgccgtaacc aattgtcgtc aataaattat ttttggataa
    91561 ttgcggtttt tcaccggtat tcatgacaat aaatgaacct gtaccataag tattcttgac
    91621 catgcctggt tcgaatgcca tttgtccgaa taatgcggct tgctggtctc ctgccatccc
    91681 tgaaattggt gcttcgcttc cataaaaatg ataaccttgc gtataaccat aaacttctga
    91741 gttagaagtg acttttggta acatcactcg aggaatattt agtaaatcta aaatctcctg
    91801 atcccaatct agctgatgga tattgaacaa catcgtccga ctggcattcg agtaatcagt
    91861 aacatgtgct tgtccatcgg tcagtttcca tactaaccaa gaatcgatcg ttccaaacat
    91921 caattcgcct ttttcagctc gttcttgcgc gccttctaca tgatcaagaa tccagcgtat
    91981 cttcgttgct gagaaatagg catcgatcac taaccctgtt ttttcgtgga aaaattcttg
    92041 atagccttca ttttttaagt tatctgcaat tccagctgac tgtctggatt gccaaacaac
    92101 tgcatgatag attggacgtc ctgtatcttt ttcccatatt actgttgttt cacgctggtt
    92161 cgttatgcca atcccagcaa tctgatttgg tttgatccct gattcgatga acgctccggc
    92221 aataactgac tgtacagaat tccagatttc atttgcatca tgttccaccc aactttcttg
    92281 cgggaaatat tgcgtgaact ctttttgaga actgccgatg tgtcttcctt tcttgtcaaa
    92341 gataattgct cgtgagctag ttgtaccttg gtcaatcgcc atgatatacg ttgattctgt
    92401 cattgtgatt ccctccatta tatgcaaacg ctttctttaa ctactcttat catactactg
    92461 ttttaaaaaa agaaactgtc aaaattttat tctttttttc tttttttgac agttttttat
    92521 tatgaagcta ttttttaatc aattcttttc ttttttcagc gtctctattc ttcttttcaa
    92581 tcgaataatg tcgtaaggtg acgcaaattc tgacagataa tattcacact gttttttgtt
    92641 tctctggcaa aatgtgatca ccaaatcata ttctttacct tcttcataac ttccaatctc
    92701 aataccgccg attcccctca gttctctgat cagatattgt tgaaaagcaa tacgataaat
    92761 cggcagactt tctaaatcga ttccaatcac tactggtttt gcaatataaa aatcgatcat
    92821 caaaagaaca ttggcatact ggatcatcag gtaactaagc tcactatctt cagctctttt
    92881 cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc agtaactgtg ttaaggaatg
    92941 cccgagcatt ttctgctgct gttccaaaag ccgatcacgc tcatagactt cgatctttcc
    93001 aacaaagaaa aaccattcat tgttgatctg ggaaatttgg tagcctactg ccttttcttc
    93061 tttgattttc aaacgacgag gacgataatg ccataacatc gtttcccgaa tgtaagtatc
    93121 taaaacagcg gttggtaact ttttgctccg tgtcaaatca tactgataaa atatttcttc
    93181 gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc attgtttcgt atacattcag
    93241 ttctgctgct gttcgactaa gatgcatcac gatgattgga tcgattgctt gataaagccg
    93301 gtccgactga tagagtaatt tcttttcttt caaagttgcg tacgtacttt tttcattcaa
    93361 tagtcttttt ttcgtaatcc cgagccagca agcaatctgt gcttctgctg atgccgtaaa
    93421 cgttgtttct aatacacggt ccaatcctag aatgaacggt cttttttctg gtgtattttg
    93481 caaaaaaagc ggtcgctgat cttccgggat atattggaat agttcataat aaaaatatcg
    93541 tatctgtagc tcttctccgt ataattgacc gtttttgatt tgtaatccaa attcctctaa
    93601 gagctggttc aattctttga tctttcgaaa aacagaagac tcactgatat taaacgctgt
    93661 cgctaatcga acgatggata cttgctgatg ttctaataaa tagacgagca tcttaaactt
    93721 caagctttcc tgcaataaaa acaatagaat tttttccaaa cttacatctt cgtccaacac
    93781 aagcagcgcc tgatactcat ttcgctgtac ttctactttt ttttccatca ttcggccaat
    93841 caattcaata tcttccaaat attgatcaaa cgaggctttt gacagttgga catgctctct
    93901 caaaccattg atagaggctg ttcccccttg cagaacaagg tttcgcaaaa tcaggatctg
    93961 taactgctct tttctttcta ataatgcttc aatcttcata ggcagtattc gccacctttg
    94021 accacaccat atctggcaca cggtattctg gctggaaaaa tagctcatgt tcttctaact
    94081 gttcaaacgt ccatccttct tctactgcaa ttgtcagttg gtcaagaagg tggaacatct
    94141 cttttttgct ttggagctgt gctccaagaa tctttttttc agaatcaaat agaaatttga
    94201 gtttaacttt gctttgattt attgctgtaa gcttgtagtc tacgtatcct gtttcaattt
    94261 tttggggata aaaaatgctc tcttcttctg tcaaacctac gcttcctaag aaatagccaa
    94321 accactgatt gccgactgga cgataggttc cctgatcctt tgtctgtgag ccactaattg
    94381 tcttgctgac atttcgtgct gtacggacag cgttatttac tagagaaaca tacatttttt
    94441 catttgttgg acgaaaagtt acttggatcg catcgccaat cgcataaacg tctttttgag
    94501 acgtttgcaa gtagtcatct acccagatcg ttccatcatc attcaatgtt aattgttctt
    94561 cccacatctg attgtcggga cgagaatgat tagccagcaa aacaagatca tagttctcct
    94621 ttcttttttc cgtctctaca atagcggttt cattttcggt caaagacaca acttgttctt
    94681 ctaaaaaaag tgaaagccct tgatttttca tttcctttac taacggttct gtcatttcag
    94741 gatctagata acgaaaaaga attgactttc ggctttcgta aagatggatt tgtttttttc
    94801 ttgatgccaa cccttcagct atttcgattc ctgcttgtcc tgcaccaata attgcaattt
    94861 tctttgcatt catgatcttt tgctgaagag ccgttacgtt ttgtatcttt ttaactgggt
    94921 gaataagcgt tgattctgca gaaatatttc gaaacttttg atttgaacca gtcgctagaa
    94981 tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac gacttttttt tctttcaatc
    95041 ctattactgt cttttctgtg taaacatgga ttcgatagcg gtcagtcagt tcttctttcg
    95101 tgatccaaga aagttgtttt tcactggtaa cctttccttt taatatcaaa ttgaacccgc
    95161 tgggaatcca tcctaacttc gattgctttt caatcaaata tatttcactt gttggcatca
    95221 attggcgaag ataaatagcc gcatgaatcc ctgcaaacga cccgccgaca attacaatgt
    95281 tcatctgcat tcctctttct tataaggtgt tctactgcta acttctcaat cagaaaacac
    95341 agtattcata actcgctttc aaccttatga tacatagcga tgcagcagaa tcaaccctct
    95401 ccatcatacc gtgttctagg tctgcttctc aataatgtac tagtggacct aattgaagag
    95461 tatcatttct gaagagatgt ttcaaaaaat gtactcttat aaaagaaaaa aactcgcctt
    95521 ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa aagacgagtt ttagcgtgtg
    95581 taatgtaata gttagaatag acacatcatt tatttttaat tctttaatgc ttcaacaact
    95641 gcttcgccta attgatgagc agattgagga ttttgccctg taagaagccg cccatcaatt
    95701 tgaaccactt ctgaaaaagc tgctccagac tgatagtttg ctcctttttc tttcaaagca
    95761 tcttctgcta agaatggtac ttcattcgtt gtaccatttg cttcctcttc ttcattactg
    95821 aacccagtca ctgtacgtcc agctaaaata gattttccag actcatctgt aactggcaac
    95881 aatcctacaa caccgtgaca aacagctgag accagccctt catttcgata gatttgtgaa
    95941 gcgactttcc caagatctat tgcttctgga aaatcccaca ttgttccgtg gccaccagca
    96001 taataaaccg ctgcataatc atttgcatcc acatcatcag gacgtaaggt atttcccaag
    96061 gcccgatttc tgaaatcttc atcttcataa taggcccaat cgatttcatc catattcaaa
    96121 acactttgcg gttctaaagg tacatatccg cctttaggac tgacaaaatc tgcatcgatc
    96181 ccatttttta ctaatacatc ataaaaatga gtcagttcac ttaaccacaa acctgtagga
    96241 cgctcgatct tttcgtactt ttctacattt gttaatacga tcaatacttt cattttccca
    96301 tcctctttct ataaaaattg cgttattgat aacgtgcttt caagaaatta tataatgggc
    96361 ttggcaaata aagctcttta tatgcttttt caaaaggttt cgttacaaat tcatcctcac
    96421 ggttttccaa taatttctct gcccagtcag gatcgacgat catttgctgt ccaattgctg
    96481 caagttctgc atatttcaat acctgctcta catcttcgcg attgcggatc ccaccgacac
    96541 caactaaagg gatctttcct tgaatcgtgt tatggaccac ttcaagaatc gatttatcgt
    96601 tgtatttatc agaacgagct attcgatcat acatatttaa tgaaacatgc agataatcta
    96661 gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc ttcaaaacgg atacctggtg
    96721 tttcatattc ttctggtgag aaacgatacc ctacgatgaa tggtttttta gcatattgtt
    96781 caaccgctga gaaaaccgtt gttaataatt cttcaataaa acgataacga ttttccaagc
    96841 taccgcccca ttgatcctct cgtctattcg aatgcggaga gaaaaattgt tggatcaaat
    96901 acgtatttgc tccatgcaac tcgattccat caaatcctga ttcaatcgct ctacgtgtgg
    96961 ctttgccaaa tgcttggatt gtttcaacga tttcttcttc actcattgct cgtggtgttt
    97021 ccgcatcagg acgttttgct gcaactgcac ttgcagatac aggctgttct ccagataaag
    97081 ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat aatcgctttt gcgccttctc
    97141 cttggattgc tttcgctagc tcacttaatc gaggaatcat ttcatcatga gcgatgctga
    97201 gttcacctgg ccagcctttt ccgatatctt ggacatttgc agcaccagta ataacagcac
    97261 caagaccttt agaacgttgt ttataataag tgatctcatc ttcggtgatc actccattga
    97321 aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg gttcttcaat tgaactcctg
    97381 aaggtaaagt aattgattga tcaaattgtg acattgtcat tcctcccata attcgctctg
    97441 attaacctat cgagaatcat ctatcattaa accactcgat tagttgtaca tgcaactaat
    97501 ttgcttaaaa aattttattt tttttgtaac ttatctgttg catcattcat tgtctgggct
    97561 aattctttac ttgcttcatt tcccaaaact gctttgtatg actgatgaag ttcttcccag
    97621 ctaccatcga cttccttagc aaattggcgt ccttgttctg taagataaac taatgacaag
    97681 cgaccttcgc taactgtttt gacgtatcct ttatattcta atttttcaac aaaacgagca
    97741 atcgttgatg gggcggaata gagcatttgt gcaatatctt tttgtggtat tccatctttt
    97801 tcttttaata tcaataaaat aaaaccataa gttggtgcca gacctgtcct tctaaacgct
    97861 tcatcagcta atttattcaa catgcgatct agctttttca cgctaaagaa aagacattca
    97921 tctaacgctt tttcaattgg cttttccatt tcatcccctc cattggcgta catatcatag
    97981 tatgattttt attttttgtc aaaaaacacg gaataatgtc cgcctcctct gtctagattt
    98041 gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt tttcgacaaa actccttctg
    98101 gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt ttgagtatga agaaaaattt
    98161 tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaaa aactggcttt
    98221 tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct gcatcaagta ataagaacag
    98281 ccaaacaaaa atagttcctc atattttgtc gttttttgag ctaatgccga tggtcagctg
    98341 attgtacacc gtttattgag aggtcgtgag cctgccgttg aactccgagt cacaaggagc
    98401 aatttcaaaa aacagcttct catgttttat gactatggtg acttgtgatc gaggagttgg
    98461 caggagaata ctgtttattc gtaaagaagg ggctgtgaca agacttttgt cacagtccct
    98521 tatttccttt tattatagga tttcgccatt tgattggatg acttgtctgt accaatcaaa
    98581 tgattttttc tttaaccgtt tctttgtgcc attcccttcg tcatcttgat caacatagat
    98641 atatccataa cgttttgaca tttgtgaagt agaagcgctg acgatatcaa tgcagcccca
    98701 cgacgtataa ccaaataaat ctactccatc tttgatagct tccttcatct gctcaatatg
    98761 ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt ccatcttctt ctactttgtc
    98821 tatagctccg agtccgtttt cagctatgaa cagtggtttt tgatacctat catataattg
    98881 attcaaagca attctcaaac ccttaggatc aatctgccag ccccattcac ttgatggaag
    98941 atacggattt ttaattcctc cagttattaa gtttccttgg accccttccc aattttctgg
    99001 atgagcactg actgcactag acatgtagta gctaaaagaa acaaaatcaa cagggtaggc
    99061 tgctaagatt tcttcatcac ctacttctat ttttaagttg acaccttttt cttcaaacat
    99121 tcgttgcgta tacgctggat aatatcctct tgcttgaaca tcactaaaga acaaagaagt
    99181 tctttgctgc tcaattgttt tttgaatatc atccggatca caagaatacg gaacaagaag
    99241 ctgatagctg atcatacaac cgacttgtga acctttgata atttcatgac aagcttttgt
    99301 agctaaagca cttgctataa actgatggtg tgcaccttgg tatttcgctt gttccaaatg
    99361 gggatggttt tcttcaatta ctcctacgct aacaaaaggg tgatgtttta cacagttgat
    99421 ctcattgaaa gtcatccaat attttacttt atttttatag cgtttgaata ctgtttcagc
    99481 gaaacgaacg taaaactgaa taagctttcg attaggccat ccaccatatt ctgtaaccaa
    99541 atataacggc atttcataat gggaaagaga aacgactggc tcaatcccat attttagaca
    99601 ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca ttgggtgttt cttcatcacc
    99661 atgaggaaaa attcttgtcc atgcgataga catccgatag catttaaaac ccatttcagc
    99721 aaataaacgg atgtcttctt taaagtgatg atagaaatca tttccgtgtc ttttaggata
    99781 ataatacttg ctgttgggat cctgcgccgc ttttacttgc tgatgcgtca tcatatgcca
    99841 ttgatctgtc catttttctt taggtaaatc gctgtcgaat gtatacacat cagacacaga
    99901 taatcctttt tcatctacat cccatgcacc ttcacattga ttggccgctg tcgctccgcc
    99961 ccataaaaat ccttctggaa attttttcat atcgatcccc ctaaacttgc tagttttctt
   100021 gtttgattat ttttcttatg actagtaatg atatttttga atctactact atttgtaaca
   100081 ataaccattg tggtcggatc atatccctct ttgattaatt cttgcaaatc tacttccatc
   100141 aataaatcgc ctttacgtac tttttttcca tcagatactg cactttgaaa gtatttgcca
   100201 tttaaattca ctgtatcaat accgatatgg atcagtatct ctgctccttc ttcactgact
   100261 accccaattg catgtttcgt tggaaaaact gttgaaactt ccccgtttac agaactgtag
   100321 atttttccgt ccgttggaat gatcgcaaac ccttctccta aagatccact agcaaatgtt
   100381 ggatcgttta cttcagataa atcaattatt tcacctgctg caggagtact taacactata
   100441 tcctttgagg attcttcttc gatttcatcc ttgtagccga aaagaaaagt tagcatacag
   100501 gaactgatca tcgctaagaa ataactagca actagccaaa caaatccttc accaatataa
   100561 atcgggaaag acaaaatact tcttgtagca actgccaaag caccggctcc tgcggcacca
   100621 gctactccac caaatatagc tccacatata caagcaatgt aaaatggtcg tttatattta
   100681 agagtcacac cataaacagc aggttcagtg attccaaata atgctgtgat aaatgcagat
   100741 aaagataaac tttttacttt cttatttttc gttttcaaaa ataccccaag tactgctcct
   100801 gcttgcgcag ctacggcagt catacatgct ggacctagcg tatcacgacc gtagaaagaa
   100861 agattgttgg tcattactgg tacaagtccc caatgtaacc caaaaatcac taacacttgc
   100921 caaaatccgc cgataacagc accagataaa acagggctaa agttataaac agctaaatat
   100981 ccagctgcaa tcagctgact tgcataatct gcaattggac ctacaactaa taaaataatt
   101041 gggaccatca cgatgataca aatgaatggt gtcaataaat ttcgaatact ttcgtgcaag
   101101 cttttgcgaa tcttaggctc aatataagaa agaacccata cagctaaaat tgctggaata
   101161 accgtacttg tataccttgc tagaatcacg ggcatcccta gaaatcgcaa ggttatgcta
   101221 tcactatatg cactgacaat cgtaggataa acgagagctg ctgcaataac catagcgatg
   101281 aaacgatctg tattgaactt ttttgcagca gtatatgcca aaaatatagg caagaaatag
   101341 aaaaatgcat cagctgctgc attcaaaatg atataagttc ctgattcagt agttaataaa
   101401 ttcaaactcg tacataaggc gagtactcct ttgatcaaac cacttcctgc caagacgcct
   101461 aaagtcgggg caaaaacagc ggcgatcatg tctataaaag agtttaaaat gccttttttc
   101521 tcagttcctc tctttccact agatgcatca tcttctagtt tcatttgttt ggataatacg
   101581 tcatacactt catttactgc atttcctact acgacttgaa acattcctcc agcttcgact
   101641 acagtgatca caccttctag ttgctctacc ttctccttat ctgccttctt attatctttt
   101701 agggtaaagc gtaaccttgt tgcacaatgg taaacattgc tgacattttc ttcgcctccg
   101761 acaaatcgga caatatcttt cgctaattgt gaatagtcca tttttacttc ccccttgtta
   101821 attaatgccg tagcaatcct tcttacaaga taagtatagt tcttagaaaa cggtttctcc
   101881 attcaattag taccccttct aatagcgaac gaaactgtac aatagtgtgt atggtttact
   101941 tagccgagca aatcactcac tgcttgaaag aaaacaagta tatcgtttct cttatagtca
   102001 aaggagacaa aaaaaacacg cttgatcttc ctttgctgat gtataagtgg tatctcaagc
   102061 tgatactctg tgatttttgt ttctacttgg cttttgataa gacagcacaa caaagtctcg
   102121 gcatttaacg gaagcataac taggtcagaa ctaggagaga ttagttgctg aatggaatct
   102181 atctttttat ggatttttat ttgattttct tctttgtagt aatgatcaag cagttttgtt
   102241 ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa tctgataaat ctcccaagat
   102301 tcaaactcat ctagaacact aggtagataa atcaaatcat cccactcaaa aataagctct
   102361 tgctcagtag tcaaaattgc atcatattcc gttattttgt ttgattgatt tttgaaaaga
   102421 tacactggtt ctatccggca ttctttgatc cacatgttca tactttgttg aatctgtttt
   102481 tttagtgtat tcaaaatact aattggctgg ttactaacaa tcagtatctc cccttgtttt
   102541 cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat aagcgctttc agctaaattg
   102601 ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta aataaatttc taaaggataa
   102661 cgggagatga tcttttgtac aaaatgattc atggtattat gtccggcatc catcctcgtt
   102721 ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat ggaacaataa cgaaacagga
   102781 agatctaaaa aacgaataat cgtttgctca aatgctctta gattcttctc tatttctttg
   102841 tttttttcat cattaatcat caaataattt aactctaata gtcgattttc aattgcttgt
   102901 ttttcttgat tggaaaaaga atagggtagt tctgatgata acttttttgt gagggtctct
   102961 accatcggaa taaataatgt atacctgttt gttttttcta aaagttcttc tcttcgaaga
   103021 atggtcaaaa cgagataccg agaaaatcgt atgaagtcct ctcctgaaac aatataatta
   103081 tatgatataa gaacctcttt taatgtcttt tggacagtcg gcatgatatt ggaaaaaatt
   103141 gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg tttctgttcc aacaagagat
   103201 aagaaaattc tttttgaatt ttcttcagca atgattttta gtccttttgt cgacgaaact
   103261 tccagttcta atttaggatt gcgctcgatc caacgcaaaa tcgtttttat ttgtaatgat
   103321 acggctgttt ttgaaagaaa gaaaatgtct gctatctttt gcatactgac ataccctttt
   103381 tgaaacaata agtacgtcac aatagttgaa gctcggaaat ccatgctgga atagatttta
   103441 tgtttcgtca cctcttgtat gatttgctgt ttagtttcag attcaattga ttctaaaaaa
   103501 taacctgaac gtttatttga ctggataatg attttcttct cgtagatagc aagcatattt
   103561 ttgatttcag tttggatcgt tttcttgttg gtacctaaag cttcagctaa ttcttcgctt
   103621 gttatccaat gattcttatt tcttaatact acaagaattt ggagttgtcg tttatttaag
   103681 tccatttgct tacacctcct ctttgttata aacatacagg aaaaacctct caaatgacaa
   103741 tagtcacttg cagaggttta aaaatcattt gaagaaagat ctatattcaa ggtttttacc
   103801 attagactgg ataatttgtt tataatattc aaaactatct tttttcaatc tcctaaagct
   103861 tccgctccca tcattttgtc tatccacata aataaagcca tatcgcttcc tcatttcccc
   103921 tgttcctgcc gatacgatat cgattggtcc ccaccataag taaccaatga tttggcaacc
   103981 atcttttatt gcctcagcaa tttcctgaag atgtattttt agatactcac aacgttttgg
   104041 atcgtggatt cgattcaatt catctggtgc ttcatctagt ccaatgccat tttcaactat
   104101 gaataatggt aattcataac ggtctgtcaa ttcattcatg acataacgaa accctagagg
   104161 atcaattggc caactccaag gttcgggaga tttttccttc aaaaagggat tatcgaaacc
   104221 atttgctccg ccagtatcta cgcgcatttc tgcttctttt ttatagacag cgctacgata
   104281 ataactaaat gcaatataat ctgccgtgtt agcttgcaag cactgttcgt ctccatctaa
   104341 cattactggt gcacagtctt cttccttcca tgtcctgtaa atataagctg gataatgccc
   104401 tcggcacatg acatcggaga aaaaccatga attgcgttta aattgcagtg aaccaaatac
   104461 attctctgga tcacagtcat atgcataagt cgctaatgca gaaaagctac acataatacc
   104521 catcttagca tttggatcga ttttctttaa taattgagta gcacgggcgt tcgctagaaa
   104581 catatgatga caggcttgat aaatcacttt ttttgttagt gtagcaatgg gttcaagttt
   104641 atctctagca gggaatgtaa ccaatccagc agctgcataa ggcatacgaa aagcattatt
   104701 gatctcatta aaggtcatcc aatatttcac tttcccttgg tagcgttcaa agacggtctg
   104761 cgcataaaat tcaaaaaagt cgattaattt tcgattttgc caccccccat actctcctac
   104821 caatgctaag ggggtttcat aatgactaag agtaattact ggctcgatat catatcgatt
   104881 taatgtattg attagtctgt cataaaatac gagaccagcc tcatttggct tctgttcgtc
   104941 tcctcttgga aatattcgtg cccatgagat gcttgttcga aaagctttta aacctaactc
   105001 cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa tcaatcgctt catgagaaag
   105061 ataatggagt gattcgtcta aagcaatctc ccaaatcttt ttttcttcat tccactgaat
   105121 actaggatgc tggttcatga tcccgataag cgtatctgtc acattaggta atttgccgtc
   105181 ttccaaccat gcaccttcac attgattagc agctactgct ccgccccata aaaaattttc
   105241 tggaaatatt tcctgatcca taactatctc tcctcgctat caacaatttc ttacttgaag
   105301 tatagctcga ttcatcaaaa caagatattc taactatttc ggcagaagag aagaaaaaaa
   105361 ttgaatgcta ctcttcattt ttaatattta tatataacaa atacataaga cgatccccga
   105421 aagaaaacac tcgttaacca tctaagcaaa cgatttattt tacttccaac acaaatgctt
   105481 cataaggtcg taaatcaact ttgccgtctg gtaactgttt cagaggataa tttgacacaa
   105541 taacccttaa agcttcacgt ggaatagtga acgtctgctt ttcactggac atattacaaa
   105601 taacgagcca ttgctggtct tcatatatgc gagaataggc aaagacatgc tcaggcgtct
   105661 tttccactaa ttcatagtct ccccagacga tgatgtcatg ttttctacgc aaatcgatca
   105721 atttcttgta catataaaaa acagaatctt gatctgctaa cgcttcttct gcattgattt
   105781 ctttataatt tggattgact cgaagccatg gaattccagt agtaaaccca gcatgttcac
   105841 gggcgttcca ttgcataggc gtcctagcat tgtctcttcc tttcgccttg attgattcca
   105901 agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt atacatattg attgtctcaa
   105961 tatcttttgc ttccgcgata tcatcgattg ggcaattcgt catgcctaac tcttctcctt
   106021 gatagatata aggggtgcct ttcatcatat ggagcaagat agcaaacatc ttggcacttt
   106081 cttttcgata cacatcgtca tttccccaac gagaaacaat tcgcggaaga tcgtgattat
   106141 tccaaaataa actattccat ccttcatcac ctaaagatgt ttgccatttg gataatactt
   106201 ttttcagttc agagatactc aagggtttca aatcccattt gtctttgcct tcttgctgat
   106261 ctaatcctat gtgttcgaat tggaagacca tggataattc atttctttta ggattggaat
   106321 aaagtttagc gatttcggta gttgctcccc atgtttcgcc aacagtcatc agatttttgt
   106381 caccaaaagt tgcttgattc atttcctgaa gatactcatg cagtttaggt ccattacttg
   106441 tgatttcttt atccggttct ttgccaatca tatcgatcac gtccatacga aagcccccaa
   106501 tacctttatc gatccagaaa ttcatcattt gataaatctc ttggcgaacc tgtttatttt
   106561 tccaattcaa atcaggttga cgcttgctat acaagtgcag aaaatattgc cccgtttttt
   106621 catccaactg ccaagcagaa ccactgaata ttgagcgcaa tctattaggc tcttttccat
   106681 tgaccggatc acgccaaata taatagtccc gataaggatt ttctttattt ttacgggcct
   106741 caatgaacca cggatgttca tcagaagtat gattcaccac cagatccatg atgatacgga
   106801 tatttctttt ctttgcttct tcaatcagtt cttccatttc ttccatcgta ccgaattctg
   106861 ccatgattgc ttgataatta ctgatatcat acccattgtc atcattcgga gactcgtata
   106921 ctggagaaag ccaaattgca tcaataccta aatacgccag ataatccaac ctggaaataa
   106981 tcccccgcaa atctccaata ccatcttgat ttgaatcttg aaaacttctt ggatagattt
   107041 gataaacaac tgaatggtgc caccattttt gttccatgat tttcctccta taaatggatg
   107101 tttctatttt ttgcttaatc atttatttta gtctctctca ctaaaaattg taacgctttc
   107161 aaatagctat tgcaccttgt tatcggtaac agaataaaac aaaaaaacag ctgaggaaat
   107221 tccctcaact gttttttgaa aggattgatt tatgcaggga agaatgctga tttcacaact
   107281 tctttataaa cggtgttcac tcagctgtcc cttggtatta gctcaaaaaa cggcaaaata
   107341 taagaaacta ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac
   107401 ctctttattt cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca
   107461 tgattggaga tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa
   107521 aacctaatgc aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga
   107581 atgaagggat cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca
   107641 atactcctac cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg
   107701 gagaaaagaa caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac
   107761 gtcctccaac ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa
   107821 ataaggaacc cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca
   107881 tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat
   107941 caaagaaagt cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt
   108001 cccctacatg ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa
   108061 tttcagaagg cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgccatcc
   108121 cgatgaagat gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa
   108181 aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat
   108241 tcgaaacgat gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc
   108301 cagaaatcgc atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga
   108361 agattgtgat caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc
   108421 atggaatccc cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag
   108481 gtgccgttcc aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg
   108541 ctaatgcagt cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg
   108601 gatttacaaa taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac
   108661 gttttaccgt ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct
   108721 tccatccccc taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata
   108781 aaacaacgtg ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcggatttt
   108841 aagcaaaatc cgaacgttcc atctgattaa atccctttat ctcaaagttc atgaacattc
   108901 actttggatt acaagaattt ctttattgtg ttaataaagt ttcttcattc tagtctcaaa
   108961 aatcaatagt gtgatttgat tttaccattt cgtttctgtt cttctttgat gaattctttt
   109021 gtatcgacag gattcagatt atttagtccg ccaaaacctg tatcgaccat ttttccccag
   109081 ccttttgtct tcttttttcc tgatttgaga ttctttgctc gaagcttctc actatgtttt
   109141 gctcgccgat acttcaaata accttctaat cttcccatta gatccactgt ttttcctcct
   109201 ttttattcaa tcagacgact cagcttttcc tataacaaaa taattgcctt cagggtcaga
   109261 aaaattgaaa gtcaagatgc cattgttatc tgtgatttct cctggttctt ccatgttctc
   109321 gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc
   109381 tggtgaaaac ttctgcacaa atacttttgg gaatacgcct aattctaatt cttttgagat
   109441 gctgagtaca atactcttaa attcttctgg caattcgatt gtttcagaaa tttctgcacc
   109501 aattttttct acaaagaatc ggcaaatcat ctctacgtca ttgacataaa gcatcacacg
   109561 tgttttttgt atcatcattt gacccttctt tctatttgat tttctttcct gtctataaac
   109621 tgattccatt atatcacgaa agcgcaaaca ccttgtgttt gatactattc aagaaaaata
   109681 aagcaatagt ttctaggatt gctttcctcc acaaactttt tttacaaaaa aaaaacggca
   109741 atgaatgccg ctcttcttat ttcgattctt ttataattct gcttctgatt catctttggt
   109801 tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg attttttcct tcaacgcttc
   109861 gattgcctgt gcgccacgtg ccgcttgttc agcattgatg gattcaatga ccagcaccgc
   109921 tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca cgccagttga gacagcgaca
   109981 gatcgctcct tgttcatcat agtagatgat ttcttccgga agtgccggag catcggaatc
   110041 tgcacctaaa ggcaggaatg attcgccacc ttgggctttt cctaaatgca gatttcctgc
   110101 gatcttttcg atatcttctc cgccgcaagg tacgccaaaa gacaaggaaa tactattata
   110161 gatatctaca agcggattga ttgggaaaaa ttcccgttct tgatttgcac gtttcagtaa
   110221 agcttcaata gaagaacgtg cacctttttt agtcttgaat gtggtaaatg cctgacgcca
   110281 ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta aggtaatttc ttgcttcttt
   110341 tgctgcctca ttcaataatt gatgatacgt ttcttcactt tcttttgtta cgtggttatc
   110401 gataccattt acggataaga tttcgatggt tgcttcagga aatacttccc agaaattggt
   110461 atccactatt acttttttca tctgttcaca cctcttgatt agaataattg cttgatgaat
   110521 agatagtctg tttggggatc atctcccata taaaaaacat gttcgctgta tttgataaat
   110581 ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt cccaaacacc gagccaaatg
   110641 gctgtttttt gcttttcttt tgctttttca atagctgtat ctagtaaaaa tgtaccgata
   110701 ccttgatgtt taaatttttt tcgtacatag attcgctcga tttctaaaaa atgatcagcc
   110761 atttcctcag attgactcgt tcctgtattt agtttcaggt aacccgcagg ctcttgatcg
   110821 atactcacta ggaaaaaatc ggtttcttta tcttttagct cttctgtaag tttttctagt
   110881 gcataagctt gtgtcaagta atcagctaaa tgctccggtg aattatcttt tccaaatgta
   110941 tctgtaaatg tttcgatact aatttgttgt agtaagtcta catctgaagg cgtcattttt
   111001 cgaatttcgt atttattcac ggcttcttcc tttcaataga tccttttatt gccttttttg
   111061 acaaaattcc aatcttcgct aacattcttg catactttat ttaaaagata ttccaattgt
   111121 tttgcttctt cgtcagaaag accatttaat gcgactgcat tggagtagtt gttttctcga
   111181 atgatgaaag gatagatttc tgctcctttt ttagtcggat agagtttctt gattttttta
   111241 ttttctttat cttccaaacg ttcgatcatc ccattggatt ctaatttctt gattgctcgg
   111301 gcagtcgttg tacgatctac tttgatcatt tctgctagtt tttcttggat gattccagga
   111361 ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg ttaaatcgac ttctttgaat
   111421 tcgatattgc taatagaatc tagcgctcgg gcaatcattc cgatgtctcg aaggatttca
   111481 gccatttcat cccaccttta ttttgtttgt aagactagtt cacctctttt tgttgcaaat
   111541 gcaactattt tcaataaaaa aatcaagttc ttttttaata actagtaaag tatattcttt
   111601 tcttctttta cgaaaaagct caatcacaat agatctggtt tttgttttct aaaataagta
   111661 actgtttctt cgttgttcgc ttttctgata gctttggcaa ctcttctata ctaaaaaatc
   111721 ccatgttgga agtttcatta ttctcaataa acttggcctc ttcaccgtgg atcgcacaag
   111781 caaaaacaag tttataatac tgaaaaagct gtggaatatc tttttgttta ttcgtatcaa
   111841 aaactgctcg caactgagta gtttctactg ttaatcctgt ttcttcatag acttctttac
   111901 gcacattttc ttcaggagac aatcctattt cagcaaatcc accaggaagt gaccattctt
   111961 tcgtccgttt atcttcgatt agtaggacct tgccatcttt tttgatatag gcacgaacat
   112021 ctacttttgg tgttggatgt ccttctccaa tcgttgtgag cgcttttatt tcttcgaaag
   112081 tttcttttcc tatttcactg atcagttcca atgaaatcgt ccggatctcc tcaaaacgct
   112141 ctttatcaaa tacatctttt ccataatata gaccagcttg agaaagtgcg agtagtcttt
   112201 tgtattgagc caacttcttg ttcattctct tcccttcttt tttagcaaat gtatgactat
   112261 gtaatcaagt atagaagact ggtttaatta cggcaaataa atataatgaa atctgcaata
   112321 aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc gacttctctg gggctgtgaa
   112381 taaatggcgt tcaaaagctt gcatctgcgg taataagccc caccaaacga aaaatatgac
   112441 gagctattct tgtctggctg ttcttattaa ttgaggcagg acagcttttt cacaacctcc
   112501 ccactaaacg gtgttctcct accaactcct cggtcacaag tcacaatatt catcaaacat
   112561 gagaagctgt ttttttgaaa ttgctccttg tgactcggag ctgaacgaca ggctcacgac
   112621 ctctcggtaa acagcgttca aaagccaatt tttcgatatt aagccatttc tttgttactt
   112681 attgtcattg atttcttttt ctaatttatc caatagtgga gtgtaatcca cttgagtata
   112741 gtctgttaca tcaaattcat atagtgttcc taactggaat ttgtcatatt ttcggtcggc
   112801 gatgatcttt ttgaatgctt ctttcgtaat atgattggtc cctttcgttc gatcttccaa
   112861 tacgtcacca tagtgataat gatccatgat atagctcaaa tgacgatctg attcacgatc
   112921 acgtttgtac cgacgttgcc ataacacttc aaagtctgct gttaggcgaa tcgtgatcac
   112981 ctgatatccg tatctatctg ctaattcttg gagtcgtgct ttttgcttat cactaaaagg
   113041 atactcggac acaatcactt tctttcctgc ttccatatac aaatcaagtg ctttgtaata
   113101 gaaaggataa actttcgtcc tttcttgttc cgcccgttct tccaaattgt taaacccgat
   113161 ggattctgca tagtattcct tgatttcatc gagcgtgatg gcatacatat ctggaaatcg
   113221 ttcccgtagt ttattcataa gatatgtttt tcctgtacca ggactaccag ccagaagaat
   113281 gaaatacttt ttcatctcat ttatttccta agttaattgg actttccaag ttcataaaca
   113341 gtccaatctt atgcttgaca atttctttta ctgcttcatt tgctggaatc acattatggc
   113401 gaagagactt gttgatttct gtttcactaa aggtttcttt agctactttc gtccagttga
   113461 ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt attgatcaat tcaggataat
   113521 cttcttcttt cacacctgtt cctccatgga taactagggg gacatcaact actgcatcga
   113581 tttctttcag caagggaatg tttacttctg tttttgattt gaattgtcca tgattcgtac
   113641 cgatagcaat agctaatgca tccacgcctg tagcttctac aaatttcact gcgtcttccg
   113701 gtcttgtata taccttgtca ttttcagcga catggattcc ttcttctgta ccgccgattg
   113761 ttccaagttc tccttctacg gaaacaccgt gcgcatgggc atattctact actgcttttg
   113821 tcttcaggat attttctttg aaaggcagat gggacccatc atacatgaca gaagtatatc
   113881 cgctgtcgat tgcttctttg atatcatcaa agtctcgggc gtggtctaag tgcaagactg
   113941 catcaaccat ttcttcttct gcaatagctg tcattacatt gactaacaca tgcatgccga
   114001 tatatttagc ggtatcgaca cttgtttgaa taatgatcgg cgaacccatt tcttttgctg
   114061 cgcctagcat agcaggaagc atttctaggt tatgcgtatt aaaagctcca actgtaaaat
   114121 tcaactcttc tgctgttttt gttacttctt tcaatgttgt atacatcttt tattgccacc
   114181 ttatcttttt atttgtttcc tttgatcgtt acttgttttg caatttgtcg catctcatcc
   114241 attttgccca tgtagtaatc gatcccttga tcatcaccag cacgagcagc atctgcacgc
   114301 ttctggttat agaggctcat cagtttttta tacccatctc cgatcgtctg tttattttcc
   114361 aaactctttt ctaaatattg gatggcttga tcgttcacat acaattcaga aaatttaagt
   114421 ccaacttgtt cataaagttt cgctaattta tgcttatctt gggtagtgga aatctcttgt
   114481 ttcaattgtg taatctctga ttgaatcttt tcggtcgctt ctggcgatag ggctttttct
   114541 tcttttgcca gaactttttt cggttctttt tcttttttct taaaaaatcc aaacatcatt
   114601 ttccctactt tcaattaaaa tgctttcaaa actgggctta agatccaggc atataagagt
   114661 gctgcggcta ctgtatctac atcggttgct gtagcattgt tgaatcccat cgaattaaag
   114721 atcgttacta gcaaagcggg caataatgta ataaagaaac catgagcaat accgccaatg
   114781 atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc ctgctgtccc tccggcaaag
   114841 aaattggtta gcataccagg taaaatcatc gctaagccga acatgggcaa agtaaacatg
   114901 gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa tgacagcatt tgggctataa
   114961 ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag gaactaattt catagctatc
   115021 cctcgaaatg ccggcacgat ttcacctaac agtagacgta caccagctag caatacatac
   115081 acacccacta cgaattggat ggcttgtaaa aaggcaaaca tgatatagtt ttgatctcct
   115141 gataattcag aagcgaatgg ttcgcctgca aataaaacag tgatgatata tagcgggacc
   115201 atcacgacca tgacagacaa ataagtatct tgcagaaatt cgaaatgtgt cggcaacttg
   115261 atatcttcga tcgacttttt attttcgcct ttctcaccaa aaagcttagc aacaccggct
   115321 tcaaataagt acccgatcgt acaaaaatga cctaaagcaa tatcatttga tccagtgatt
   115381 ttacgaatga tcggttgcgc tactgccggc atagcaatgg cgaaacaagc tccgataaaa
   115441 ccaccgacca agatcaatac gatccctcgc agaccggcaa aatagccaaa gactgtcgtc
   115501 atcgttgcca tccataaaat tgcttgacca gttaaaaaaa tgtacttcca ttttgtgaaa
   115561 cgagccaaaa taatattgaa tacaaaaatg gctaagaacg tcagtgcaat atctctacct
   115621 aaacccaaat cattcattgc ttgaccattg atagattcaa tagaagggat gatcccttgc
   115681 atatgaaatc cttcagtaaa gattttcccg aaataagtta aacttccaac gatgatgctc
   115741 gaaccggcac tcaacacttg aaatcctagg agtgtcttta acgtgccaga gatgacttgt
   115801 cctgctgatt tcttctgcag tagtagaccc aacatagcaa tcaatgcaat ggtgatcgat
   115861 gcctgagtca aaatattttc gatgataaaa ttgataacgc ccatgtt
//
LOCUS       DAYYBH010000003        83777 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000003 DAYYBH010000000
VERSION     DAYYBH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.003"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(202..942)
                     /locus_tag="ACHAUG_000220"
     CDS             complement(202..942)
                     /locus_tag="ACHAUG_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479346.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1072..2019)
                     /locus_tag="ACHAUG_000221"
     CDS             complement(1072..2019)
                     /locus_tag="ACHAUG_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8479347.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2148..2912)
                     /locus_tag="ACHAUG_000222"
     CDS             complement(2148..2912)
                     /locus_tag="ACHAUG_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8479348.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2916..3305)
                     /locus_tag="ACHAUG_000223"
     CDS             complement(2916..3305)
                     /locus_tag="ACHAUG_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479349.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3440..3982
                     /locus_tag="ACHAUG_000224"
     CDS             3440..3982
                     /locus_tag="ACHAUG_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8479350.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4130..4762
                     /locus_tag="ACHAUG_000225"
     CDS             4130..4762
                     /locus_tag="ACHAUG_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479351.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4896..5021)
                     /locus_tag="ACHAUG_000226"
     CDS             complement(4896..5021)
                     /locus_tag="ACHAUG_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8479352.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5155..6048)
                     /locus_tag="ACHAUG_000227"
     CDS             complement(5155..6048)
                     /locus_tag="ACHAUG_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8479353.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6230..7156)
                     /locus_tag="ACHAUG_000228"
     CDS             complement(6230..7156)
                     /locus_tag="ACHAUG_000228"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8479354.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7241..8002)
                     /gene="pflA"
                     /locus_tag="ACHAUG_000229"
     CDS             complement(7241..8002)
                     /gene="pflA"
                     /locus_tag="ACHAUG_000229"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8479355.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8246..10477)
                     /gene="pflB"
                     /locus_tag="ACHAUG_000230"
     CDS             complement(8246..10477)
                     /gene="pflB"
                     /locus_tag="ACHAUG_000230"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8479356.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10754..13204)
                     /gene="parC"
                     /locus_tag="ACHAUG_000231"
     CDS             complement(10754..13204)
                     /gene="parC"
                     /locus_tag="ACHAUG_000231"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8479357.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13216..15261)
                     /gene="parE"
                     /locus_tag="ACHAUG_000232"
     CDS             complement(13216..15261)
                     /gene="parE"
                     /locus_tag="ACHAUG_000232"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8479358.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15427..15861)
                     /locus_tag="ACHAUG_000233"
     CDS             complement(15427..15861)
                     /locus_tag="ACHAUG_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8479359.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15988..16626
                     /gene="plsY"
                     /locus_tag="ACHAUG_000234"
     CDS             15988..16626
                     /gene="plsY"
                     /locus_tag="ACHAUG_000234"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8479360.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16758..17633)
                     /locus_tag="ACHAUG_000235"
     CDS             complement(16758..17633)
                     /locus_tag="ACHAUG_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8479361.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17715..18506)
                     /gene="codY"
                     /locus_tag="ACHAUG_000236"
     CDS             complement(17715..18506)
                     /gene="codY"
                     /locus_tag="ACHAUG_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8479362.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18524..19924)
                     /gene="hslU"
                     /locus_tag="ACHAUG_000237"
     CDS             complement(18524..19924)
                     /gene="hslU"
                     /locus_tag="ACHAUG_000237"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8479363.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19938..20486)
                     /gene="hslV"
                     /locus_tag="ACHAUG_000238"
     CDS             complement(19938..20486)
                     /gene="hslV"
                     /locus_tag="ACHAUG_000238"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8479364.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20523..21428)
                     /gene="xerC"
                     /locus_tag="ACHAUG_000239"
     CDS             complement(20523..21428)
                     /gene="xerC"
                     /locus_tag="ACHAUG_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8479365.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21626..23002)
                     /gene="trmFO"
                     /locus_tag="ACHAUG_000240"
     CDS             complement(21626..23002)
                     /gene="trmFO"
                     /locus_tag="ACHAUG_000240"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8479366.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22971..25049)
                     /gene="topA"
                     /locus_tag="ACHAUG_000241"
     CDS             complement(22971..25049)
                     /gene="topA"
                     /locus_tag="ACHAUG_000241"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8479367.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25171..26028)
                     /gene="dprA"
                     /locus_tag="ACHAUG_000242"
     CDS             complement(25171..26028)
                     /gene="dprA"
                     /locus_tag="ACHAUG_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8479368.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26084..26851)
                     /locus_tag="ACHAUG_000243"
     CDS             complement(26084..26851)
                     /locus_tag="ACHAUG_000243"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8479369.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26844..27704)
                     /gene="ylqF"
                     /locus_tag="ACHAUG_000244"
     CDS             complement(26844..27704)
                     /gene="ylqF"
                     /locus_tag="ACHAUG_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8479370.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27964..28362
                     /locus_tag="ACHAUG_000245"
     CDS             27964..28362
                     /locus_tag="ACHAUG_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8479371.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28599..29141
                     /locus_tag="ACHAUG_000246"
     CDS             28599..29141
                     /locus_tag="ACHAUG_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8479372.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29224..29775)
                     /gene="lepB"
                     /locus_tag="ACHAUG_000247"
     CDS             complement(29224..29775)
                     /gene="lepB"
                     /locus_tag="ACHAUG_000247"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8479373.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29927..30154)
                     /locus_tag="ACHAUG_000248"
     CDS             complement(29927..30154)
                     /locus_tag="ACHAUG_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8479374.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30151..30669)
                     /gene="msrA"
                     /locus_tag="ACHAUG_000249"
     CDS             complement(30151..30669)
                     /gene="msrA"
                     /locus_tag="ACHAUG_000249"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8479375.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30689..31402)
                     /locus_tag="ACHAUG_000250"
     CDS             complement(30689..31402)
                     /locus_tag="ACHAUG_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8479376.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31405..32301)
                     /locus_tag="ACHAUG_000251"
     CDS             complement(31405..32301)
                     /locus_tag="ACHAUG_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8479377.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32459..33301)
                     /locus_tag="ACHAUG_000252"
     CDS             complement(32459..33301)
                     /locus_tag="ACHAUG_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8479378.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33433..34086
                     /locus_tag="ACHAUG_000253"
     CDS             33433..34086
                     /locus_tag="ACHAUG_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8479379.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34149..34676)
                     /locus_tag="ACHAUG_000254"
     CDS             complement(34149..34676)
                     /locus_tag="ACHAUG_000254"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8479380.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34696..35643)
                     /locus_tag="ACHAUG_000255"
     CDS             complement(34696..35643)
                     /locus_tag="ACHAUG_000255"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8479381.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35655..37538)
                     /locus_tag="ACHAUG_000256"
     CDS             complement(35655..37538)
                     /locus_tag="ACHAUG_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8479382.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(37701..39779)
                     /locus_tag="ACHAUG_000257"
     CDS             complement(37701..39779)
                     /locus_tag="ACHAUG_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8479383.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39792..40067)
                     /locus_tag="ACHAUG_000258"
     CDS             complement(39792..40067)
                     /locus_tag="ACHAUG_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479384.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40429..40884)
                     /locus_tag="ACHAUG_000259"
     CDS             complement(40429..40884)
                     /locus_tag="ACHAUG_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479385.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(41038..42246)
                     /locus_tag="ACHAUG_000260"
     CDS             complement(41038..42246)
                     /locus_tag="ACHAUG_000260"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8479386.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42358..42690)
                     /locus_tag="ACHAUG_000261"
     CDS             complement(42358..42690)
                     /locus_tag="ACHAUG_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8479387.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42838..43713
                     /locus_tag="ACHAUG_000262"
     CDS             42838..43713
                     /locus_tag="ACHAUG_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8479388.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43795..44376)
                     /locus_tag="ACHAUG_000263"
     CDS             complement(43795..44376)
                     /locus_tag="ACHAUG_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8479389.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44382..45641)
                     /locus_tag="ACHAUG_000264"
     CDS             complement(44382..45641)
                     /locus_tag="ACHAUG_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8479390.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45643..46689)
                     /locus_tag="ACHAUG_000265"
     CDS             complement(45643..46689)
                     /locus_tag="ACHAUG_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479391.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46924..47199)
                     /locus_tag="ACHAUG_000266"
     CDS             complement(46924..47199)
                     /locus_tag="ACHAUG_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8479392.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47442..48752)
                     /gene="der"
                     /locus_tag="ACHAUG_000267"
     CDS             complement(47442..48752)
                     /gene="der"
                     /locus_tag="ACHAUG_000267"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8479393.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48934..50163)
                     /gene="rpsA"
                     /locus_tag="ACHAUG_000268"
     CDS             complement(48934..50163)
                     /gene="rpsA"
                     /locus_tag="ACHAUG_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8479394.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50298..50978)
                     /gene="cmk"
                     /locus_tag="ACHAUG_000269"
     CDS             complement(50298..50978)
                     /gene="cmk"
                     /locus_tag="ACHAUG_000269"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8479395.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(51027..51662)
                     /locus_tag="ACHAUG_000270"
     CDS             complement(51027..51662)
                     /locus_tag="ACHAUG_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8479396.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51728..53131)
                     /locus_tag="ACHAUG_000271"
     CDS             complement(51728..53131)
                     /locus_tag="ACHAUG_000271"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8479397.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(53118..54149)
                     /locus_tag="ACHAUG_000272"
     CDS             complement(53118..54149)
                     /locus_tag="ACHAUG_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8479398.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54180..54401
                     /locus_tag="ACHAUG_000273"
     CDS             54180..54401
                     /locus_tag="ACHAUG_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8479399.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54566..55303)
                     /locus_tag="ACHAUG_000274"
     CDS             complement(54566..55303)
                     /locus_tag="ACHAUG_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8479400.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55456..56796)
                     /locus_tag="ACHAUG_000275"
     CDS             complement(55456..56796)
                     /locus_tag="ACHAUG_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8479401.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(57033..58556)
                     /locus_tag="ACHAUG_000276"
     CDS             complement(57033..58556)
                     /locus_tag="ACHAUG_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8479402.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(58934..59545)
                     /locus_tag="ACHAUG_000277"
     CDS             complement(58934..59545)
                     /locus_tag="ACHAUG_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8479403.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(59668..59793)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(59875..60591)
                     /locus_tag="ACHAUG_000278"
     CDS             complement(59875..60591)
                     /locus_tag="ACHAUG_000278"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8479404.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(60597..61166)
                     /gene="scpB"
                     /locus_tag="ACHAUG_000279"
     CDS             complement(60597..61166)
                     /gene="scpB"
                     /locus_tag="ACHAUG_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8479405.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(61150..61920)
                     /locus_tag="ACHAUG_000280"
     CDS             complement(61150..61920)
                     /locus_tag="ACHAUG_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8479406.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(61917..62288)
                     /locus_tag="ACHAUG_000281"
     CDS             complement(61917..62288)
                     /locus_tag="ACHAUG_000281"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8479407.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(62307..63194)
                     /gene="xerD"
                     /locus_tag="ACHAUG_000282"
     CDS             complement(62307..63194)
                     /gene="xerD"
                     /locus_tag="ACHAUG_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8479408.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(63290..63763)
                     /locus_tag="ACHAUG_000283"
     CDS             complement(63290..63763)
                     /locus_tag="ACHAUG_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8479409.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(63868..64731)
                     /locus_tag="ACHAUG_000284"
     CDS             complement(63868..64731)
                     /locus_tag="ACHAUG_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8479410.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(64927..67026)
                     /locus_tag="ACHAUG_000285"
     CDS             complement(64927..67026)
                     /locus_tag="ACHAUG_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8479411.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(67246..68355)
                     /gene="rpoD"
                     /locus_tag="ACHAUG_000286"
     CDS             complement(67246..68355)
                     /gene="rpoD"
                     /locus_tag="ACHAUG_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8479412.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(68379..70220)
                     /gene="dnaG"
                     /locus_tag="ACHAUG_000287"
     CDS             complement(68379..70220)
                     /gene="dnaG"
                     /locus_tag="ACHAUG_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8479413.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(70402..72717)
                     /locus_tag="ACHAUG_000288"
     CDS             complement(70402..72717)
                     /locus_tag="ACHAUG_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8479414.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(72902..73762)
                     /locus_tag="ACHAUG_000289"
     CDS             complement(72902..73762)
                     /locus_tag="ACHAUG_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8479415.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(73821..74453)
                     /locus_tag="ACHAUG_000290"
                     /pseudo
     CDS             complement(73821..74453)
                     /locus_tag="ACHAUG_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            74620..75381
                     /locus_tag="ACHAUG_000291"
     CDS             74620..75381
                     /locus_tag="ACHAUG_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8479416.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            75407..77998
                     /gene="mprF"
                     /locus_tag="ACHAUG_000292"
     CDS             75407..77998
                     /gene="mprF"
                     /locus_tag="ACHAUG_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8479417.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(78186..79373)
                     /gene="tuf"
                     /locus_tag="ACHAUG_000293"
     CDS             complement(78186..79373)
                     /gene="tuf"
                     /locus_tag="ACHAUG_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8479418.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            79741..80097
                     /locus_tag="ACHAUG_000294"
     CDS             79741..80097
                     /locus_tag="ACHAUG_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8479419.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(80338..81342)
                     /locus_tag="ACHAUG_000295"
     CDS             complement(80338..81342)
                     /locus_tag="ACHAUG_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8479420.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            81508..82359
                     /locus_tag="ACHAUG_000296"
     CDS             81508..82359
                     /locus_tag="ACHAUG_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8479421.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            82376..82621
                     /locus_tag="ACHAUG_000297"
     CDS             82376..82621
                     /locus_tag="ACHAUG_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479422.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            82808..83512
                     /locus_tag="ACHAUG_000298"
     CDS             82808..83512
                     /locus_tag="ACHAUG_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479423.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        23153 a        17550 c        14372 g        28702 t
ORIGIN      
        1 aataaactat ttttgcaaac gatctgcgaa aatagtttgt ttttttattt tgccattatt
       61 ttcttactta ttgggagaaa aaattcgctt aaactcaaaa aaatttgagg atacgatata
      121 aactttcgga tagagtataa aataaggaaa cgccgcagcg tttcctttcc aactgtagcc
      181 gaatataaat ttgtatatca cttatttaaa atagctattt tctcccaaag agtttccttg
      241 atcatcgatc aatccttgta atgagcgttc taagatacca tggctttttt tcgatttttt
      301 ttcttctgac tgctttgttt cctctaacgt ttttgttcga ttgaatgttc gtacagacgg
      361 ctcaccattt cttttttctt gatacggcgt cggttctgta tcgaacaaca gataagagtc
      421 atccgttttt ttcatcgatt cgattaaatg atcctcagaa attttgcttt ctctagggtc
      481 tgggataatc gatgcgggta catatttagg aacaaaataa gaacgctccc gcaccttcgt
      541 gtcaacagat gcgtttcgtg tagtatgctt ggactctgtc cttgtttcac tttgtctgac
      601 agaacgagag gaatcaccaa aaagtttttt tcgtcctgtc gatgtgattt ctgattggta
      661 atggccggta taatccggta attttccttt atgcttttcc aaattttcct tacgcggagc
      721 tgacttcaat ccatttgttg cacgacgctt gcgacggatt ttctcggtgt tcgtgttcgt
      781 gaaatgaaat tctttcatct gttcaaacgg ggactgattc tgttcagtca aaagatcttc
      841 ttgtccgtca aataatactc gcttcttgtc gttggttaat tttacgccta actcatcatc
      901 gtacgcagga aaacggaaat gttttcgtct gatttctttc attcaaacca tccttctcaa
      961 aaatggaact tttctcaata atagcacaga ttcattgttt tcagtagcaa tgcgctgatt
     1021 attttaagaa aatagcgatg aatttcataa atatatatat agtaataaga cttaattcgt
     1081 tgtctctctg ataactagtt caggaaaaaa acgacgataa ccttgactcg cccctttttc
     1141 ttctattttt tttaacaaca tttctccaca tacttcaccc atacgtataa tatcttgctt
     1201 gatcgttgtc aatttaggtg aactgatttg gtctaaaaat accccatcaa atccaatcac
     1261 accaaattct tcgggcaagt gtcctccagc atttataatc cctcttactg ctcctagtgc
     1321 tagccgatct gaactacata caaaacaagt tttatctgta aaatctgccc agttttctgt
     1381 catatatctt tctacttctg tcgaatgatt tggaaaccgc aagattttac taggaagatc
     1441 aacttccttc atcacagata aataaccagt ttcccttgaa atttcaaaag gctcgtccac
     1501 ttcgataccg atataaataa tcgtttgata atctctttct aaggcatatt gcgttgcttt
     1561 tgctgtacca tattcattat cgctgtctac ataatcatat ccttcagaat tttccccaaa
     1621 caagacaact ggtttttcca aacggtcgat ccaatcgaag tctttttttc tgacacctgt
     1681 gatgatgtat ccatcacaag aaccgacgtc gaaggcatca tttgtaacta attggagaga
     1741 ataatgcgct ttaccgatcc gtttagcgat tcccatcagc aaattcatgt aatagggctc
     1801 agttgtgtcg atttcttcca gtataaataa tttgatgatc tgcgaccgat tcgtgacaag
     1861 tgcttttgca gcaatatttg gacggtaatc cagttcagcc attgctgaga aaactaactc
     1921 tttcaattcg tcagtcacct gttccggatg attgatgact cgagagaccg tcatttttga
     1981 aacattggca cgttttgcaa catctgctaa agtgggcatg ctctcttcct ttctaacatg
     2041 ttgtcattct tactatcaat tataccaaat tttcctaaca gagtttatta tagcccaaaa
     2101 taaaaaacgg aaaatcctct atcgtcatga gtgattttcc gtttcaatta tttatacttt
     2161 tttatcaaat tttttgctaa aaaagtggaa gcttcttcca ttagtggtat ttgtaccata
     2221 tgtcgctgtg tttttgagga acgaaaaaca atttcttttc cgtaagactt atgccgattt
     2281 ttttctacaa atcgttccgt ttgtatataa gggatttttt cgtcagcttg tccatgccaa
     2341 aagaacagag gacgtccagc taacttttcc ggctgcaatg acaaatcata gctcttaacc
     2401 catgaaatca aatcaaaata gtcttttggt acgacataat tatattccga tgcgcgcctt
     2461 acgatttctt ttccatattc caaaggtgca ggggaaccca tcaaacacac gcccgctact
     2521 atatgaggat gtttagccaa caaggcgcat gttgtcattc cccccatcga aaccccgcca
     2581 actgcaagct gatcatgtag aagacctctt ttttctaaat gcttgataag tgtgtcaaat
     2641 tcaaacagat ttccgtagat actatcccaa aatgtatagg atggaataga agaaaccggc
     2701 tgtttgcgct ccccatgatt catcgcatct ggcaggatca cacgaatgcc ctgctgcgca
     2761 atttttcttc cttgcgtcaa aactaactcc ttacttgtct gccatccgtg ataatagatg
     2821 acaacgggaa tacgttcctc tcttaattct ttcagaacaa cttctaacac tggaatatga
     2881 tggacagaac gtttcaatat aattggattc aattgttact cacctatttt cattaaattt
     2941 cctgatcgcc atagcaacca tgctccaaca gttgtctgga acaggttcag tgtaccaaat
     3001 aatataaatg caggactgaa tggctcggga aataagcaca gagaacaaag tcccaccact
     3061 cctaatgcta gcgaaaggag tggataaata gaaaaattca aaaaactggc aacgctaccg
     3121 aaaatattac caataaacag tatcaaaaag aaaaaaacgc ttaaaattaa accatctgtc
     3181 agcgacatct gaaaaacaat gttctctacc tcaattggct gttgaatcga atagacacct
     3241 aagtatatac tgagaaggag acttagcacg tggctggcac aaaacacacg attgaccgtt
     3301 ttcattttaa tcacctaacc tatctttata attctattat aatcatttta tcattttttt
     3361 gtatgtataa agtttcaatt tttcttcact tacgattttt tttgatattg ttgttatata
     3421 aatgaaagga gttttacaaa tgattgatcc aaaaatgttg gggatgatct tcctcattaa
     3481 ttttgcttat atcactctga acacgttacg tttcatgttg acaatgaaag gctatcgact
     3541 gattgctcca cttgtcagta tggtcgagat cacgatttat atcgtcggct tatcccttgt
     3601 tttagatcgt ttagataatc cactgaattt atttgtctat gcgttaggtt acgcagtagg
     3661 tatcagtgtc ggaattttga ttgaagacaa attggcatta ggatacatca tggtcacaac
     3721 gattttgcca gcaaatagcg cagaagataa aagtttgccg ggtatactga gagaacacgg
     3781 ttatggtgtg acacagactt ctgcaatggg gctggaaggt gagcggctag ttctcgaaat
     3841 actttctcct agaaaaagcg aacgtgaact gtatcgcctg atcaaagaaa tcgaagagcg
     3901 tgcttttatc atttcttatg agccaaaata tatttcaggt ggtttctgga ctaaaaaagt
     3961 gagaaaacga caaggaaaat aactcacaaa cttaaaaact gtctaatatc taaattaaat
     4021 tttaaatgtt agacagtttt tatttattat tttgattaaa aaacatcgac atatactcat
     4081 caatttgtta ttatacttta tatacttatc aatttaattt ggagatatta tggaaattca
     4141 acgtagaacc actaattttt tacatttagg ctcttttttt gcaactttga cgatcttatt
     4201 ttttctatgt tattcatacc ttaagacctc acatgcgcct tctagtgcat taggctggtt
     4261 gcttttggat cattctgata tcacagaagc cgaagaaaat ccgttttttt tgattttgtc
     4321 ttctctttgc ggcttactgt tttctgttta tttcggtgcc tatttttttc atcgacatcc
     4381 tggtacgtta aacgaagctt caagcgtatt gattgcattt aaaacgtttt cttttttagc
     4441 aattcttttt tattcgttta atcacaatac gcttttcttc tttatcatga atggtttagt
     4501 cgtgatttta tccattttta cttattttat tttttcactc atttttagcc aattgaaatg
     4561 tccgctcttc tttcgattag ttcctgtcac ctgttttatt tacagcttgt tttccttttt
     4621 tgtccttgct ttaatgccac tgaccaaccc ttcgacgatc caagtcgcag gaaatctgtt
     4681 ggatatgctc ttcatcgtca gtctaggtgt atttatgtgg caaagaaaaa acaaacaaaa
     4741 taaaaagcat tttgaagcct gactattacg acttcataaa tgctttttta gttttaaaca
     4801 cttcaatcta aaagaataac ccaagattgg ataaattatt aatgactcgc cattcgtttc
     4861 ttagtgattc tggattatca aatggacttc ctaatctaat cagctagttc ttcccataaa
     4921 atgccatact tgactgaatc ataatattga ttatttaaaa atctaacttt tcttatcact
     4981 acttcctttg tcattccaca tttctcacca tttttttgca ttcctttgtt tccagaccat
     5041 gtagtgaatc taataggtgg tagataggca aaatactcaa ataattcttt taaccataaa
     5101 ggaagagaaa ggctcctcct gcaagagtca gtataatttt tgcttactgc tctcttaggt
     5161 tttactcact aaaactgttc catattcttt gatataagaa aatgcatgcg gtataaattt
     5221 cttgctcagc tcagaaaaga tttgtcgttt aagaaaggct atttcctcca ttgtcaatga
     5281 aggatccaat gttggtaatt caatctcaaa attgtttttt tcgacaatag gtaattctaa
     5341 tgtgaatgat tcttcttcct ttatagtatt cgtcacaagt agagactgat ggaaaatact
     5401 aggccggcga tttttatatt tattcttacg tatcctgtca acgataactt ctatttgatc
     5461 aaaaaagtac gattcattta cgtctccaat aagttcggct aattctttga atgccttcgt
     5521 tacaggaagc tgattcttct gtacaaaaaa gtaattagcc aaagtaagcg ggaagtgttc
     5581 tatagaggaa agtgtgataa aacggtactt ctcatttcct gcagttgtcc tcagcaattc
     5641 tgtctctcgg ctgtttctta ctccataaaa atacgcttct ttccgttcat cttcgaaaca
     5701 atacgcccaa ataacttctc ccatcgctaa tttttgttca tcaacagaga gtctcttcaa
     5761 ttgttcagct atggtttcag cagccgaagt ggcttgttgc aagtaatctt taggatcaaa
     5821 tattggaaag ttcaaatgat atcttcgttc ttccctcttg attaatcctg catcgatcaa
     5881 taaatcaaga aaatgggaaa atttattttg cgaaaattct tttttcaatt ctcttagaat
     5941 cgtttcttgt tctcgatgtt catatagata atcggtcaat gaagaaaaaa gcggatcatc
     6001 ggtcagtttt tgataaaagt tttccttatt ttttgaataa tagttcatat atgttctctt
     6061 ttctatccgt tttattttac ttaaaaggct tttacgtaaa aaggaggaag aacaagagtc
     6121 ataattgact gttattctcc tccatttctt cggcctttag tatgttctat gtttcttttt
     6181 atatcaatcg cttatccatt cattttgaat gcttcatact accatttgat tagctaaata
     6241 cagccgtcaa ttgaggtacg acttgttttt tacgtgaaac gacaccttta agcaaagcac
     6301 ggttgttggt caaagttgtt tggaaagctt cttctacttt ttcttttcct ttaccagcaa
     6361 caagtaattc tgaatcactg tctaagatgt ttgtgacaac taatacgaac agatcgtagc
     6421 catttgcttt catttcatct tccattgctg cttctagctc tgcttgtcga tccatcactt
     6481 cattaaggtc cactgtattg acttgagcga tacgaacgtt ggaactgccc attgggaaac
     6541 tttttgcatc caaatcaaga agaaccgcag ctgatttatc acttagattt gttcctgctt
     6601 tcaacatttc aagtccataa gtgtttagat cgacttcagc gattgctgca agttcttttg
     6661 cagctgcttc atcttcaggc gtacatgttg gtgatttgaa caacaatgta tctgaaatga
     6721 tagctgagag catgattcct gcgatttctt taggaattgt tacttggttt tctttatata
     6781 gttttaaaat aatcgtactt gtacatccaa ctggttctgc acgatagtac aatggattag
     6841 ctgtttcaaa gtttgcaata cgatggtggt ctactacagc taaaatattt acttcgctga
     6901 tatctgaaat actttgttgg aattcattat gatccacaag catgacatct gcattttctg
     6961 ctttagctga ctctactact cgtggtgcag atagctgaaa gtggtctaat gcgtattgtg
     7021 tttcttcatt aggtgtacct agagcaactg cttctgtttc tttacctaat tcattttgca
     7081 agtgtgaaaa cgcaatggct gccccgattg catctgtatc tggattttgg tgaccaaaaa
     7141 ctaaaatttt tgacatgtca ttcgctcctt tgtgatttct cctatttaat aatagcaaaa
     7201 aaacggaaag aagcaatact tatcttcttt ccgatgcgac ctatcgcgtt aaatattttg
     7261 tatactcttc tgtatgaagc aatcgtttag cattttcgac acgttctttt gtaggtggtt
     7321 cgatcccttc taatggatat ttcaaaccaa gtgtttccca cttgtatttt cccatcgtat
     7381 gataaggaag aatctctact ctgtctacat ttgacaaggt ttgaataaag ttgtctagtc
     7441 ggatcaaata ttcatcataa tcacttcgct caggaactag tacatggcga atccaaaccg
     7501 gtttgttgat ttttgacaaa tattgagcca tttctaaaat attttcattg tcccaatgcg
     7561 ttagtttttt atgctcttca ttatcgatgt gtttgatgtc aaataacagt aaatctgtat
     7621 acttcatcaa ttcttgaaaa cgtgaaaaga aaggttcttc atacgtaaaa ggttttccgc
     7681 atgtatccaa tgtagtatgc atcttccgct cttttgcttt tttaaatagt tcgatcaaaa
     7741 aatcgatctg caatagaggt tctccaccac taacagtaat accaccttta tctccccagt
     7801 aaggtctgaa gcgttctgct ttatctaaaa gttcatctgc tgtgtattct ttccctccgc
     7861 caatattcca agtatctgga ttgtgacaaa attggcaacg catgcggcat ccttgcataa
     7921 acacgacaaa gcgcagtcca ggtccgtcta ctgaaccgaa agtttcgata gaatgaacat
     7981 agccgattgt tttttcttcc atttaagttc acatcttttc tttgatttca ttttaagtac
     8041 aagttagctg tcgaagcgaa gctttctgaa caaaattttt cgcaattgcc gcttcgttat
     8101 gtgtaaaatt tacagggacg gaacatacaa gtcctgcccc tgtaaatcgt ttctttcaaa
     8161 taagcatact gattattcga ttttgatgcg atggctattc aacgtccttt cgacgctaag
     8221 tcgaaaaact tttttcacga actttttaca ttctgtcgtg tgaagttctc atcaagacat
     8281 ctaattgttg ttctcttgtc aagtcacgga atttaactgc ataaccagat acccggatcg
     8341 tcaagttcgg atatttcatt ggattttcca ttgcatcacg caataaatca ttactgaata
     8401 cattgacatt caaatgataa gctccttttg agaagtatcc atctaagaca ttacgcaagt
     8461 tttcgattct tgtatcttgt tcttttccaa gacctgaagg attgattgtt tgggtatttg
     8521 aaattccatc caatgcacag ccgtaattca ttttagctgt tgagttcagg ctagctaata
     8581 acccactttg ttccgcattg taacttggat ttgctcctgg cgcaagtggt tttccagctt
     8641 cacggccatc aggagtattt cctgtatttt taccgtaaac aacatttgaa gtaatcgtca
     8701 ataatgaagt tgttggtttt gagttgcggt aagttttatg acgtttgatt tgtcccatga
     8761 agtattccaa gatccatgaa actaattcat ctgcacggtc atcatcattt ccgtatttag
     8821 ggaagtcgcc atccacttca aaatcaactg ctattccttt ttcattacgg attgctttta
     8881 cttttgcata tttgatcgct gacaagctgt ctgccgcatg agagatccca gcaatacctg
     8941 tagcaaatgt gcgttgcaag tcagaatcca tcaaagccag ctgagctgct tcataagaat
     9001 atttatcatg catataatga atgatgttca acgtattgac atatagttct gctaaccaat
     9061 ccatcatatt catgaagtta tgtttcactt cttcaaagtc tagtatttct gatgtgattg
     9121 gacgatattc tggtccaact tgggcgccag tcacttcatc gactccgcca ttgatggcat
     9181 ataatagtgt tttagccaag ttagcacgag cgccaaagaa ttgcatgtct tttccgacaa
     9241 ctgttcctga tacacagcaa gcaatcgcac aatcatcaga accccattgt tcacgaagct
     9301 gattatcatt ttcgaattga atcgaagaac tttcgatagc gattctgcta gcatatgttc
     9361 tgaatccttc tggaaggtgt tcagaataca aaacagtcaa gtttggttct ggtgaaggtc
     9421 ccatatttgt taatgtatga agcagacgga aatcattctt tgtaactaat gaacgaccat
     9481 ctaatcccat acctgcaatg gataaagtag cccagatagg gaatcctgag aataattggt
     9541 tatattcagg tgtacgcgcg aatttgacca tacgtaattt cattactaag tggtcgatca
     9601 attcttgtgc ttctttttct gtgattactc cattatccaa atcacgttgg atatagatat
     9661 caaggaatgc tgagatacgt ccgattgaca tcgcagcacc attttgtgat ttgattgctg
     9721 ctaaatatcc gaagtatagc cattggacag cttcttttgc atttgcagct ggtttagaaa
     9781 tatcatagcc atatttcgca gccatttctt tcatttgcgc taatgcacga tattgttcag
     9841 tgatttcttc acgtaaacga atcacttctt cactcattgt atgataacca gtcatatcat
     9901 gatctttttt cttttgttcc atcaaatagt cgattccata caatgccaca cgacgatagt
     9961 caccgatgat acgtccgcga ccgtaagcgt ctggaagccc tgtaatgatt ttgtttttac
    10021 gagcagcacg catttcagca gtataagcgt caaatacacc ttgattatgt gttttgcgcc
    10081 agtcagtgaa aatatgtgtc aattcatcgt ttggtgtata gccattgctt tttagcgcat
    10141 tgttagccat attgattccg ccgaacggca tgaatgcttg tttcaaaggt ttgtctgtct
    10201 gtagaccgac gattttttcc aaatctttat ccaaatagcc tgcatcgtgt gaagtaatag
    10261 ttgatacgat ttctgtgtcc atatcataga caccgttacg ctcgaattgg atatcattta
    10321 acttttgcaa ttcatcccat aatttttctg ttgcttctgt tgggccttcc aaaaagctgt
    10381 catcaccgcg atattcagtg tagttatttt gaataaagtc tctagtgtct acgccttctt
    10441 tccatttggt acctttaaat ccattccatt gctccattgt atggacctcc taaatttctg
    10501 ttttatgtaa cagtttttac gtgattagta taacacagca ccctatcttt gcaagggttt
    10561 tcttgttttt tatttaacaa tttttatgtt ttatttacat atatttaaat caacgttgac
    10621 ataccaacat ttaagcattg tgaacacttt catctttttt tgcaaacgta taaggattta
    10681 ttaaaaatga atgaaaaaga gacgccacct gtattaatac agatggcgtc tcttcattaa
    10741 aatactacaa cagttacgac tcgatgaaaa tctcaggagc atcgataact tcgatgactt
    10801 ctccaccttt tttctcatcc atcgcaaatg atccattcga tgttcgatca cctataggga
    10861 aatcagttgg gtcaatcgtt aactgctgcc ctttttgatt catcaaggtt atttcttttg
    10921 atcttccttc cgacatatag ataatacggt gcggattttt tttcagctca cgcatgaccg
    10981 tcactccccg cttcgcacgt gaagtttggc tgatttcttg agcaagcatg cgtttaacag
    11041 ctcctcgctg agtaacgatt acgatcggcg tatcaccttc tgctaacaca agcagcccgt
    11101 tgataatcct atctccatct ttcaaattca tagacttcac tccagctgcc tttgctccaa
    11161 caacgggaac atctgttaaa ggatagcgca aacccattcc ttgatgcgtt actaagaaaa
    11221 catctaagcc tggctgatct tctttcagat acacagcaat cagttcatct tcttccgcct
    11281 ttagcttcat acagctgagc ggacggctct tataggtgcg ccatggttcg aactctgtca
    11341 ttcgtgtttg cttgatcaat ccattcttgc tgatgaatac aaatgttttc tctgcttgaa
    11401 ttttttgata tgggaaaaca gctaaaatcg attcgtctac agacagattg gagattgttt
    11461 gagaaatgtg ctcaccagca tctttccatt tcaaatcggg tagttcatgg acagggcggt
    11521 agatcacatt tgctttattt gtgatcagaa gaacatggtc caatgtactc aattcacctg
    11581 agtatagcaa gtagtcgcct tctcgcatcc cgatttcttc tggtttggaa gcactataag
    11641 aacgaaggct tgttcgcttc acatagcctt ctcgcgtcac actgacaatt acgtcttctt
    11701 gtgccactaa gacttgcgta tcaattttga tttcttcgat ttcgtcttcg attgtcgtta
    11761 gacgagcgga agaataattt tttttcactt ctcgcaattc ttttttcata acagagaaaa
    11821 gctctttgtc atttgaaagg attttattca actctgtgat ttgcgcaatc aagctttcgg
    11881 attccttttg aagctctgtg atatccgtgt tcgtcaaacg gtataattgc aatgtgacaa
    11941 ttgcttctgc ttgttcttct gtaaattgaa agacgtcgac taaattcttt ttggcgtctt
    12001 ttttgtcttt actttcacga atcgttgcga ttacttcatc cagaatcgat aaagctttca
    12061 tcaatccttc tacgatatgc tgtctttttt gagctttggc taaatcgaat tgactacgtt
    12121 ttgttatgac ttgctttcga tgctcgatat aacttcttaa aatgtctttc agtccaactt
    12181 gatgcggagt catatggtcg atagcgacca tattgaaatt gtaattgatc tgtagctcag
    12241 tgtttttgaa caaataattc aaaattcctt gagcattggc gtcttttttc aattcgacga
    12301 caatttgcag acctgttcta tcactttcat ctcgtacttc agctattcca tcgattttct
    12361 tattcaaacg gatctcatcc atttttttca ccaaggtcgc tttatttact tcatatggga
    12421 tttctgtgat cacgatttgc tgtttattac ccttcattgc ctctatcttt gttttcgaac
    12481 gaagaataac ttttcctcgt cctgtttcat aagctttttt gatttcatct tttccttgca
    12541 agatccctcc tgttggaaag tcaggtccag gaatatattc catcaatttt tcaaggctcg
    12601 cttgtggatg atcgatcata tagattgtgc catcgatcac ttcagctaag ttatgagtcg
    12661 ggatttctgt tgcatatcca gcagatatac ctgtcgatcc attgacgaga aggtttgggt
    12721 atcgagctgg aaggactgtt ggttcttttt ctgtatcatc aaaattccac acgagatcga
    12781 ctgtttcttt ctcgatatct gctagcatct ctccacttaa cttggataat ctggcttcag
    12841 tataacgcat cgcagcaggt ggatcaccgt ccatactccc gttattcccg tgcatttcga
    12901 tcagtacttc ccgcaacttc cagtcttggc tcatgcggac catcgcttca taaatactga
    12961 tgtcaccatg aggatgataa ttccccatga tatttccgac agattttgct gatttacgga
    13021 atcctttgtc atacgtattg ccatctttgt tcatggcaaa taatatccgg cgttgcactg
    13081 gcttcaatcc atcacggata tcaggcagtg cacgttcttg gataatatat ttggaataac
    13141 gtccgaagcg gtcacccatc acttcttcta aagttaattc ttgtacttct tgtcgatttt
    13201 ccatcaggtg acctcctatt ctacttcaaa taactgattg ttttcatttt tatctgctcg
    13261 ttcttcatct aataaatcat tagaaactga aggagagatt tcagtatcct cttttttatc
    13321 taagatactg ccatcttctt cgagcgtaaa ttgcacgtga ttttcgatcc attttcttcg
    13381 aggttctact ttgtcaccca tcagtgtggt tactcgacgt tcagcttgcg cagcatcatc
    13441 gatacggacg cggatcaacg tacgggaagt cggatccatc gttgtttccc acaattgttc
    13501 cgcattcatc tctccaagtc ctttatatcg ctgcagcata tatcctttgc cgactttttt
    13561 aatcattgct gccaattcgt catctgtcca cgcatattca atgacttgct tctttccttg
    13621 tcctttggat actttatata aaggcggtaa cgcgatatat actttcccag cttcaatcag
    13681 cggtttcata taacgataaa agaaagtaag cagtaatact tgaatatggg cgccgtctgt
    13741 atccgcatcg gtcatgatga ttactttgtc gtaattacag tcttcgatag aaaactcagg
    13801 accgactcct gcaccaatcg tatagatcat cgtattgatt tcttcatttt tcaagatatc
    13861 ttgcatttta gctttttcgg tattgatgac ttttcctcgt aaaggtagaa ttgcttggaa
    13921 ctttctgtct cttccttgtt tagcagatcc gccagcagaa tccccttcga ctaaatacaa
    13981 ttcatttttc ttagggtttc gggattgtgc gggtgtcaat tttcctgaaa gaagagattc
    14041 gcctttttta cgcttcttcc catttcggct ttcttctctg gctttacgtg ctgcttctct
    14101 cgcttctctg gctttgattg ctttacggac caacatctgg ctcatttcgc tattttcctg
    14161 caaataaaag cccatttgtt cactgatgac gttatctact acggtacgag caactggcgt
    14221 accaagtttt tcttttgttt gcccttcaaa ttgcagaaga ttttcaggta cacggataga
    14281 aagtacagca gctaaccctt ctctgaaatc actaccttcc agattcttgt cccgttcttt
    14341 cagcaaacca acttttctcg catattcatt ataagatttc gtcatcgccg ctttcatacc
    14401 agcttcatgg gttcccccat cttttgtccg aacgttattg acaaaagata aaacattttc
    14461 tgaataaccg tcattgtatt gataagcgac ctctacttcg ataccttctt tttctcctga
    14521 aaaataaaca actggtgtca atgtatcttt ttcttcattc agataatcaa caaattcttt
    14581 gattccttct tcgtaatgaa aaatttcctt gactggttct tctccacgaa gatcagaaag
    14641 agagatttta acgcctttta gaagaaaggc agattctctt aaccgttcgg ctaaaatttc
    14701 atatgaaaat ttcgttgtac tgaatattgt atcatctggt aaaaaatgga cagtcgtccc
    14761 gtttgctttt tttgtttttc ctatcttttt caaagtaccg tcaggtttac cgccgttttt
    14821 aaatttctgc tgatactcta cgccatctct aacgatcgtt acaattagcc atttcgataa
    14881 ggcatttacc acactggctc ctacaccgtg cagtccacca gaagttttgt accctccttg
    14941 accaaacttt ccgccagcat gcagcacagt aaaaatcact tcaactgttg gtattccgga
    15001 agcatgcatt cctacaggca tcccacgtcc gctatctgca acagttatac tattgtcttc
    15061 atggatggtc acatcaatct cactgccata acctgataat gcttcgtcaa ctgcattatc
    15121 gacgatttca tagactaaat gatgcaagcc tcggctgtca gtggatccaa tgtacatccc
    15181 tggtcttttt ctaactgcct ctaatccttc caatacttgg atggacgagt cattgtattc
    15241 gttgtttact ttttttgcca aggcaaacgc tccttcaata atactttgcg gcaaaaccgc
    15301 accacgatta tcatactaga aaaggtcgtg aaaaaaaaga gggtagacga gaaatcaatt
    15361 ttaattatct cctaaccgct ttttcgacat tcccctctat cgatcatctc ttctttgtgg
    15421 tcatttttat ttttggtttt ctttcaactt accaagttca atcttaatac aacgatccat
    15481 tacgatatct gtacgtcctg cttttcttag catctcagct gcttctttgt tttcaatccc
    15541 taattgtgcc cagaacactt tagcatcagt tttcaaaaaa tcacctgcta tttcaggtaa
    15601 aaattcactc cgtctaaata catctacgat atcgattgga tcatcgattt ctgtcaaatt
    15661 cgcatagact ttctcgccta gaatttcttc accagccaat aatggattta ctggtatgat
    15721 tttatatcca ttgtcttgca atacctgtgc aaccatgtaa cttgttcgtt ctggtttatt
    15781 gctcaagcct acgacggcaa tattttttgc ttcttccaaa tagttgaaaa tcgtttcttg
    15841 tgttggattt tttacagaca tttctgccac cccttgcttt tctttttatt ccatctatta
    15901 tacttgtatc tttcattttt tcaattgttc ctgttaccta ttcatgctaa aataaaggat
    15961 agttttcaac ttgaaagagg gtaaaaaatg aaattgatct tattacttat tttagcatat
    16021 cttttaggtt ctattccttc cggtgtctgg attggcaaat tgtttttcaa aaaagatatc
    16081 cgacaacacg ggagcggtaa tactggaacg accaatacat ttcgtgtatt aggcaaaaca
    16141 gccgggattg ttgtcctgct tatggatatt ttaaaaggta cattggctac ttgtttgccg
    16201 atgatcttcc acctttctgg gattaaccct ctttggtttg gggtatgcgc cattcttggc
    16261 catacatttc cgatttttgc tgggttcaaa ggaggaaaag ctgttgcgac aagtgcagga
    16321 atgcttttag gatttaatcc tgtatttttt gtttattctt gtatcatctt tatcgttagt
    16381 ttattttgta caagtatggt gagtttgagc agtatgatca gtgcagtatt gatcacttta
    16441 tctacgatcg tccttcctta tgtagctcct gtgattttgg ctaagcccaa ttggttactg
    16501 acgatcattg cctttttagt ttcttccttt atcttttatc ggcataaaga caatatcaag
    16561 cgtatcagaa accatactga aagtcgaatc ccgtttggtt tgaatagcag caagaaaaaa
    16621 tcctgatact ttttcattcc aaagagataa aacgggagtg tgacaaatcg atgtcacact
    16681 cccttattac ttacaggata gcttttttac acaccttggt aaacggtgtt ccctgctaac
    16741 ttctcggtca caacctctta cttcactgtg atggaatatt ttgtcttgaa gatttcatgg
    16801 gcacctagtt gattgatccc tactttttcc atcaagtttc cactactgtt caccgtgtca
    16861 gcgattccgc accacggttc gatacaaacg aaaggcgctt cttttggata aggagaccag
    16921 atccctacat aaggcatatc tttatagctt aaagttacgc tatgggaaga tttttcactt
    16981 cgaatggcgt acgcatttag accccttgtt tcaaaaacca aggcatcttg ctcaaaaagt
    17041 tcacgattca aagccagatt cgtatttgtc tgacctaacg tcttttgatc catatcgatg
    17101 aatgggcctt tcaatggtag gcgtaatctt gatttcaatg gagaaaatgc cagatagaaa
    17161 tcttcaaatg acaatccttc ttcaagaggt acattgaaag caggatgtcc accgatagaa
    17221 aagaacattt ctttttcacc ggtatttgcc acttgataat gaaccgtgat gccgtctcct
    17281 cctaattcat agctgatcac taattcaaaa tcaaatggat acttttctaa tgtctccggc
    17341 gtactcctta agatgaaact agccatttct tgtccatgtt caatgacttc aaaatccata
    17401 tcccgagcaa atccgtgttg ccccatttga tatgttttcc cttgataaac atactgatca
    17461 tcttttaaac gtccaacaaa agggaataaa accggtgcat gtctgcccca atacgctgga
    17521 tcaccttgcc agatatactc taaaccatgt tcttttgatt tcagactgat caattccgca
    17581 ccgtgttcag cgatttcggc gatcagctgg tcattttgaa taataacagt cattgccatt
    17641 cctccaactt cgacttactt tttaaaatgg gaaggctgga acaaacattt ctatctgccc
    17701 cagtcccctt acttctaata actgtgtttt gctaactcgt ctttaaaacg ggaattcaat
    17761 acttttaaat aagttccctt catgcctaac gaacgagatt caatgattcc agctgattcc
    17821 aattttcgta aagcattgac gatcacagaa cgagtgatgc cgatactatc ggcaatatta
    17881 gaagcagtca gacgaccttc atctccatcc aatgcttcaa agatagcttg tacagccttc
    17941 aattcgctat aagataatgt gttgattgcc atttggacag ccgttgcact ccgtacatct
    18001 gcttcgatgc ttcgagactt ctgataaagg atctgcatac cgacaaccgt agctccatat
    18061 tctgctaaga ctagatcgtc atctgcaaac gattgttcca tacgagataa gataatcgta
    18121 cccaaacgtt cgccagctcc aaagatcgga acgacagtcg tcagaccaaa tggatatttc
    18181 tctcttgact ctaccggaaa tgcagtaata tcactagtaa ttgggatatt agcttcagtt
    18241 ttcatcaact gtgccactgt atcagtataa gattcaggaa attgtttctc aacaaacatt
    18301 gttttgattc gttcattgtt cacatcgtga cgttcattat aacctagtaa tactccgtca
    18361 cttcctatga tataggtgtt gctgtcaaga ataccaccta gtgtgacagc cattttatcg
    18421 taaggcaaat ctgcttgcat gtcaaaagta ttcttttgct gcaaaagtgt gtttatcttg
    18481 cgggttttcg ttagcaagtc tgtcattgtc gtcctccgat tttttataaa atatagcgac
    18541 ttaaatcttc atttgccgca attgagccaa gtttgtcatc cacatatgct tctgtgatcg
    18601 ttatttctcc catttgcata tctggtgctt caaataatag atcttctagt agtttttcca
    18661 aaatggtatg cagtctgcgt gcgccgatat tatctgtgtc acggttgaca tcgaaagcga
    18721 tttgagcgat ccgttcaatt gcttcttttg taaacgtaac tgacacattt tctgtaccga
    18781 tcaacgcgac gtattgtttt actaaagcat tatttggttc ggtcagaatc ttcacaaaat
    18841 cttcagctgt caaatcatct aattctaccc gtatcgggaa gcgtccttga agttctggaa
    18901 ttagatcact tggtttagac aaatggaaag ctcctgaagc aatgaatagg acgtgatctg
    18961 tttggatgat cccgtatttt gtgtttactt gtgaaccttc gacaattggc aatatgtctc
    19021 tttggacgcc ttctctggat acttctccag attgctgtga tttagaagtg atcttatcga
    19081 tttcatcgat aaaaataatt ccactttctt ccgctaattt gattgcctca ctatggatat
    19141 cagcatcctt gactaatttt gctgattctt ccttgatcaa taactctaaa gcttctttga
    19201 ctgttaccgt ccgttccact tttttcttcg gtgtcaaagc acctagagtt tcatttaggt
    19261 cgatccccat ttgctccaat ccattgttca tcattggtgc aggtttcttt tggtcttcaa
    19321 cttcaatggt tacttcacga ctatccagta aacccttttg tagttgttcc aagactgtac
    19381 ggcgattgat tcgaatatct tctgtcactt cttcttgcgg ttcagcggta ttttgctgac
    19441 tagcattaaa aagattcatc atttgttcaa attgattagt ggattgtttc tgttcttttt
    19501 tgataccagg taccaagatt tttaccaaac gtttttctgc atttttttta gcacttgtgt
    19561 atacacggct gtactgctct ttttctacga tttggatcgc attttctact aaatcccgaa
    19621 ccattgattc tacatctctt ccgacataac cgacttcagt aaattttgtt gcttcgactt
    19681 tgacaaatgg tgcattgacg atttttgcca aacgtcgtgc gatttctgtt ttaccgaccc
    19741 ctgtaggtcc gatcatcagc atattttttg gtgtaatatc ttgttgcatt ttttcatcta
    19801 actgcaaacg gcgatagcgg ttacgcaaag caacggcaac agattttttt gctgcgtgct
    19861 gtcctacgat atattcatct aattcactta cgatttgtct tggtgtttgt tttaattcat
    19921 acatatctac tcacctctta cagttcttct acgataatat tatgattagt aaagatacag
    19981 atatctgcgg caatatttaa cgcattttct gcaatttctt tcgcactcat gtcgttttga
    20041 gcatagtttt tcattgctcg ggctgcagcc aacgcaaaat tccctcctga tccgatagct
    20101 aagataccat catctggtgt gataacttct cctgtacctg aaacaagaag catttcttta
    20161 tcattcatta cgatcagcat cgcttcaagt ttttgcatag attgctgtgt ccgccattct
    20221 tgagctaatt cgacagctgc gcgttgtaga ttcccgttat attcgtttag tttgccttca
    20281 aatttttctt ctaaagtaaa cgcatctgca acacttccgg cgaaacctac aacaacttct
    20341 ccattgtaga tacgtcgtac tttctttgca gtgcctttca tgacaacaga ttcacccatt
    20401 gttacttggc catctccagc catagcaaat ttcccgtctt tttcgacggc acagatagtg
    20461 gttgaatgaa atggtgattc gaccatgttt gtttcctcct aaatttgttc ttttccttat
    20521 ttttacgctc ttggatggaa agaacgatag tttttttgta aactttcttt tgttacatgg
    20581 gcatagatct gggtagtcga taaattggca tgtcctagca attcttggac tgtccgcaaa
    20641 tctgccccat tatttaataa atgagtagca aatgtatggc gcagcatgtg aggatggata
    20701 tcactatcca aactgctttt tttgatcatt tgattcaaga catattcaat tccagtaggc
    20761 gtgattggtt ttccatgatg attcacaaaa acgatatcat gctcttcgtg atattttgtc
    20821 atcaatacct ggcggccttc cctgaaatat tcttgcagag catcttgagc aaaagaacct
    20881 agaggagcat atctctcttt gttcccttta ccatggatca acatgacctc tgaatcaaaa
    20941 tcaatgtctg ataccgacaa actgctacac tcacttaaac ggatgcctga cccgtaaagg
    21001 acttccagta aagcgcggtt cctcaaatcc aaaggactat ctcctgctac gctgtcaaaa
    21061 agtgcttgca tttcattttc ataaaaaaaa cgtggcagtc tcaaattttt ctttttgaga
    21121 tgaacgtaag aaaatggatt ttcttttatc acttcttctt tgagaagata ctgataaaac
    21181 gaacgtaggc tggctatctt gcgactgata gaatttctgc tatagctagg acgatgatca
    21241 gctaatgcac tcaaatagac acgaaccgta aaatattccg ccttcagcag atcagcgtct
    21301 ccagattcct ccaaaaaatg tttgaaatgg agaatgtctt cttcatatgc ttctttcgtt
    21361 ttttctgaat atcctctttc gacaatcaaa taacgcaaaa atttttgttc ccacgtggct
    21421 tctgtcattt tctcaccttc cttttgtccc agagaataat ttagcacagc gtttataaaa
    21481 agacaaataa attgtcagaa tttaaccaat atttctaagt ttgttcacaa ttggcgcaat
    21541 tcctcacatt ttgtttgaga aattcttaag aaaaacgcaa gaaaaagagc gagactatcc
    21601 gtctcaccct cttcatggag tttccttact catttgggat tgcagttttt tctaattctg
    21661 tttttattgc ctcattagct tttaaagctc gttgtgcaat tgcttcatat cttgcttttt
    21721 tgtctcgaat gcgctctggc agttcaggaa acagaccata gttggcattc atcggttgga
    21781 aatgttttcc ttccgcatga gtaatgtagt aagccatact tccaatagct gtttcttgtg
    21841 gaaactcgac tggttccatt tcttttgcta agcgtgcggc gttgattcca gcaagtagtc
    21901 cgctcgctgc actttcaaca tagccttcta caccagtcat ctgacctgca aagaacagat
    21961 cttcgcgttt cgttgattgg taagtcggtt tcagcaattc tggcgaattc ataaagctgt
    22021 ttcggtgcat cacaccatag cgaacgaatt cagcattttc taacccagga atcatccgga
    22081 aaacacgttt ttgctcgccc catttcaagt gagtctgaaa gcccacgatg ttgtataaag
    22141 aagctgcagc attgtcttga cgcagctgga taacagcata aggacgtttg ccagttttag
    22201 gatcttccaa tccaactggt ttcataggtc cgaaaagcat cgttttgaat cctcgctgtg
    22261 ccattacttc aattggcata catccttcaa aatatttttc tttctcaaaa gatttaagtt
    22321 ctgccacttc ggcattgatt agtgcctcat ggaaagcttt gaattcctct tctgtcatcg
    22381 gacagttcaa gtaagctgct tctcctttgt catagcggga cttcaaatat actttatcca
    22441 tatcgatcgt agctttgtct acgattggtg cagctgcatc atagaaataa aaaccatctg
    22501 aaccattgaa tgctttgatg ctttctgcta atggatgaga agtcaaaggt cctgttgcta
    22561 ctacggtgat tccttccggt atttcagtaa tttcttcgtt tatcactgtt actaatggat
    22621 gctctttgac ttttcgggta attgtttcag aaaaagaatc gcggtcaaca gctaaggcac
    22681 caccagctgg cactgctgtt tcatctgccg aagtgattac taccgaattc aatcggcgca
    22741 tttcttcttt caataaccct actgcattag ccaaactgtt tcctcttaat gaattggaac
    22801 atactagttc agcaaaatta cttgtgtgat gcgcctcagt cgatttgact ggacgcattt
    22861 catataaacg gacagggact cctgcttgtg cgatctgcca agcagcttca cttccggcaa
    22921 gccctgctcc aacaacattt accattttca atgaaagaac ctcttttcta ttatttttgg
    22981 atgtcttctt cgtaatctcc atttacacag actacttgtt taccgccttt gactttcttc
    23041 tcgaccagat atccgtcaca ttttggacaa tttctgccca ctggtttatc ccaagaagtg
    23101 aattcacatt ctgggaaacg ggaacaacca tagaatagtc tgtttttctt agattttcgt
    23161 tcaatgactt gtccttcatg acatactggg caagtcacac cgatttcttt tacgattggt
    23221 ttcgtattcc gacaatcagg aaaattactg caagcataaa acttcccata tttccctaat
    23281 tttatcacca tcggatgtcc acagacatca caatcgaatc ctgctggttc atctttgatt
    23341 tgaatttttt cgattttttc ttccgcattc gtcagttctt tttcaaacgg tttatagaaa
    23401 cgatcaacca ctttgaccca cgcttccgtg ccttcttcaa ttttatccaa atctccttcc
    23461 atttctgctg tgaaatggat atcgacgatt tgagggaaga attcacagat taatgaattg
    23521 acaatttctc ctagttcagt cggctcaaat cttttttgtg tcaattttac atagtagcgt
    23581 ctttggatcg tatcaagtgt tggtgcataa gtggaaggtc gccctacccc gttttcttct
    23641 agtgcacgaa tcaacgtagc ctcactaaaa cgtgctggtg gttgagtaaa atgttgtttt
    23701 ggttcaatat caacagattg gactacatcg ccttcttcaa gttctggaag aatattttct
    23761 ttctcttctt tgccgtcatc tctaccttcc acatacactt gcatgaatcc tttgaatttg
    23821 atttttgaac cgttcgcaat gaagatgaca ccattttgtt ctaatgtcac tttcattgta
    23881 tctaaaatag ctggtgtcat ctgactagcc acaaaacgtg accagatcaa tgtataaagt
    23941 ttcaattgat ctttatctaa atattgtttg agttcatctg gtgtacgaag cacactcgtt
    24001 ggacgcacag cttcatgggc atcttgtgcg ccttgagcat ttttgatctt tcggtgatca
    24061 tgtctagaaa attcttttcc atacgtatct tcaataaatg ctgcggcctc tgcttttgct
    24121 gtatcggcaa tccgggtaga atcagtacgc atgtatgtaa tcaagccgac tgttccttgt
    24181 ttacccaaag tgattccttc atataattgc tgtgctacca tcatggtttt tcttgttcga
    24241 aagtttaact tacgggcagc ttcttgttgc aggctacttg tagtgaaagg attcgcaggg
    24301 tttcttttgc gctctttctt ttctactttt ttaacttcga agtctgttcc atcaattcta
    24361 gcagtgactt cttttacact ttgtgcatct ggtaatttct ttttcttacc gtcgattccc
    24421 cagaaatttg ctttgaattt cttgcgagct tttttaaaat taccgtcgat gctccaatat
    24481 tcttctggaa caaattctcg gatctctttt tcacggtcaa taataatttt taaagcgaca
    24541 gattggacac gtcctgcact taaccctttc ttgaccttac gccagagaat aggactgatc
    24601 gaataaccaa cgattctgtc taaggtacga cgagcttgct gtgcatctac taaatctaca
    24661 tcgatcgttc ttggttcctt aaaagctgct ttgactgcgt cttttgtgat ttcattgaag
    24721 accacccgat ttttttcttt cagatccaac ccaagtaggt acgctaaatg ccaagcaatc
    24781 gcttctcctt ctctatccgg gtcacttgcc aagtaaactt tttgagcttt ttttgctgcg
    24841 gctttcaggc ttttgatgac atcgcctttt ccgcgtatag aaatataatg gggttcataa
    24901 ttgttttcaa aatcgatacc cattttactt ttcggtaaat ctcttatatg gccaacactg
    24961 gccattactt tatagtttcg tccaagatat ttctcaattg tcttggcttt tgcaggtgat
    25021 tctacaatca ctaaatattt atacgccatt actgaatgga ctcctttcca ttgtcgtgtt
    25081 cctactatta aaagaggatt catacaaatc gtagctaatc atatccattc agaaaacgtt
    25141 tgtcaagaaa agcaaatgaa aagtatggtg ttattcttta aattctgcca agatatcttc
    25201 acccgaaacc gtgcaaactg caccgtcttg gatcaaatga tgacatccgg tagaatgttc
    25261 tgttaaaata tttcctggaa cagaaaaaac tgttcttcca agatccaatg cttgctgtgc
    25321 tgttatcaga gagccgcttt ttttcttggc ttcaaagaca catgttcctt tagacaaagc
    25381 tgcgataatc cgatttctca ttggaaaaca atgctttttc gggccggatt gaggaggata
    25441 ttcactcaaa agaagatgat catttgcaat cacctcttgc aagtcacgat tttcagcagg
    25501 atagacttgc tctatccctg ttccaatgac tgcgattgtc cgtccaccat gagagattgc
    25561 tgcttggtga ccgtatgtat ctatcccttt cgcgcagcca ctgacgatca catatccagc
    25621 ttctatcact ggttttagta aataataaac tgtttttttg gctaaagcag aagtgtgtct
    25681 tccaccaaca acgcttaaca tgttacaaga aagtaaagat agatcaccat tataaaacag
    25741 tacaattggc ggataggtca agtgaagcag ctgaggggga tatttgggat caaagcaagt
    25801 gataaacgac tgtttcgatt ggattttgcc ccagttagcg tgaatttcct gccaactttt
    25861 gcgaaacagc tcccggtgtt tggttatttt acctatctta atgatgtctt caaatccgat
    25921 ttctttataa ggatactgca tcatccagtt agcaagtgcc cattttcctt gactactgat
    25981 tccttttgta taagcaagtt tcaataaatt ttcttctatt tgatacatca taaaaccgcc
    26041 tttcgtctct ctttctaata agatacgtaa aagcggctgt tttttattgt aaatattttt
    26101 ttacaggtgc aaaactttta cgatggattg gcgtaattcc ttgtcgttca agtccttcta
    26161 agtgttcctt tgttccataa cctgcattat gcgagaaacc atatccagga taaagcacgt
    26221 catattcttt catcaattgg tctcgatata ctttagcaat gatacttgca gcagcaatcg
    26281 acacagatcg tgcatcccct ttgattattt tttcctgctg aataggcaaa tccaaggtca
    26341 tcgcatcgat caacaaatag cttggtacaa tggacaattt ttctactgct tggatcatcg
    26401 ctaatttact tgcttcataa atattcacat catcgattgt ctgctgatca acgatcccga
    26461 tcccgattgc cagggctttt tcttgaactt gttgataaag aagttctcgc tttttctctg
    26521 ataattgttt ggagtcattc agccctaata tgatctcatt tttcggcaag ataactgcag
    26581 ctgccacgac aggtccagct aatggtcctc ttcctacttc atcaatgccg gcaatccatt
    26641 ggtgaccttg tgcgtaccct tgcttttcaa agcaaaacat ttcttcatat ttttccttaa
    26701 ttaatgcttc ctttttcaat cgtcgttcaa aagaagccag tgcttgctgc acgcctttgc
    26761 gtggatcatt tcttaatgat tctagtgtag gatcatctat tctctgtatg cttttcaatc
    26821 gttctttgat actttggatg gattcagtca tcactttttt gctccatttc ttcgcaacga
    26881 tccagtgtgt atcgtccaag ttttccttga cgaatatcat gcacgactaa ttcgctgcca
    26941 cgatcatagt cttctttata cccacgtttt gctgtaatat ccatcagtaa ttccggtgcc
    27001 aatttttctt cttgctcgat tgataaacca taacgatcgc tgattcttcc tgaataatat
    27061 cgggcaaaaa agtctaatcc gtaaatcgct aaatcatcta aatggacaag ctggtctttg
    27121 atcgcgcctg tcagcgctag ttttttgccg atttcttcat cttcaaattt gggccataaa
    27181 atccctggtg tgtctaacaa ttccaactca ttacctaaac gaagccactg ctgacctttt
    27241 gttacgccag gcttatttcc tgtctgtgca attttcttgc ctactagtcg attcatcaac
    27301 gtagatttcc caacatttgg aatacctaag accattgccc gaatagcgcg aggcttgatc
    27361 ccccgagcag catctcttgc ccgtttttct ttcaatgctt ctttagccgc aggtactaga
    27421 cctttaattc ctttactttc ttgtgccgtg atagctaatg caagatggcc ttgtttacga
    27481 aaatattctt gccacagctt tgtctgttgt ggatctgcca gatccgcttt attcaataaa
    27541 acaaggcgag gtttttgctg aatgatctga tctagcattg gattacgaga agataaaggt
    27601 aatcgtgcgt ctaccaattc aaatacgatg tccacatatt tcattttttc agaaacttct
    27661 cgtcgggctt ttgccatatg tcccggaaac cattggatgg tcattgttct ctccattcct
    27721 ttcataccgg aatattcccc ggctttactt gtttttttct ttttcaatat aaattcactt
    27781 ttttattata aagaaattcc atctattagt taagtgaaag tgaaccagtc ctcattctat
    27841 cacatcttca cttttttgat gatgcaaatt tttattttgg tttctatata tgaatctaaa
    27901 tttttgacag cgtttcctaa ataagagatg atagacgaac aaaacagaaa ggagacgaaa
    27961 caaatgaata gtatggtttt tgttaatttt cctgttgctg atgtcaatcg ttccgttgct
    28021 ttttatgaaa agctgggttt taaaaaaaat gaagaatttt ctactgaaga agctggttca
    28081 atggtatggg atgacacttt ttggataatg cttctctctc atgattttta taaaaaattt
    28141 ataaaagata aagaaatagc tgataataaa aaaacaagtg gtgctttgat ttcgtttagt
    28201 gttgaaaatg cggatgaagt aaaaaaattt gctcaaattg caaaagaaaa tggtggtact
    28261 ttccacgaag tagatatggg aatgcctgaa gaagaaatgt tttcattaga agtccaagat
    28321 ctagacggaa atacattaga acccgtatgg atgaagatgt aagcagataa ctaatctgac
    28381 gttttttata acaaaaaaac aaaggcggat gatacgataa gtattctccg cctctatttt
    28441 ttttattcgt acaatctctt ttttacagtt tataaaagca gtcttctcac ccttttttta
    28501 attgttagat gccagtgtat gtaaatcacc aatgtgtcat gacacattgg tgattttatg
    28561 gtatcctttt attattaact gacaaaggag agaatatttt gaacactttg attgcaaaag
    28621 acgtttttag attgcccgaa atgaatgcaa gattgttgtc gacctctgct cttttattag
    28681 cccttgatgt catcttatac aagatagcga ttggtcctgc ttattttcaa atcacattcg
    28741 gatttttaag tatggcgctt atgggttatc tatatggacc gatctgggct ggtttgcttg
    28801 aaatgttgtc gaacacttta aatttcacga tatttggttc aggaacgttt caagctgctt
    28861 ttcttatcac ggctttcctt ggtggattta ttaatggctt atttttatat cataaaaaga
    28921 taaacctgcc ttccgtcatc gctgcccaat tattgattat gatcccagtc agccttttta
    28981 tcaattcttg gctgatgtct gttttgtttg gtgcagattt caaagtaatt tttctaggta
    29041 gattcatcag gaatatgatc caaatcccta ttcaaataat ggttgtttat tcatttatca
    29101 aaactttaga ggctagaaaa gtgtttaaac gagctgtttg attttacgta ctaaggtagt
    29161 aaactgattt tggattaaac aataaagcgt gcagtctcaa cagactgcac gctttattgt
    29221 ttctcactgg atccatttta tttcatcaag tggataatag acaaatcttg ctttacctag
    29281 aatatcagtt ttattgatcg tgccaaacga acgactatct tttgaaactc ttcgattatc
    29341 tccaagtaca aaataattat cttccggaag cttggattgt cccgttagct cttgtagtgt
    29401 aaagtccgtc gtataggatg catttgcatg atctgatttc aggtttttag ttagataagg
    29461 ttccttaact actttcccat tgatcttcaa ttgatcattt tggtagctga cattttctcc
    29521 tggaagaccg attactcgtt tgatataaat cgtgccatct gctaattgaa aaacaacaat
    29581 atcaaatcgt ctgatctccg aaaatttttc catcacaacc atatcacctt gcttcaacac
    29641 gttttccatt gaattgcctt ccactggtac cgggatgaat aaaaatcccc gcaacatgaa
    29701 cgcaaggaat acagaaacaa gtaagtattt gaatacaagc cagaagcgat taatatagcg
    29761 ttgttttttt gtcaatctct atcaatccat ccctcttttt ttcattcttt ctttattata
    29821 agaaaaatat caaaaaaatg cgactagata gctgaaataa aaataattct ttcaattagg
    29881 ctattggaca aaaaactttt ttgatcataa agtacaaaac tatctcttat atgtcatgat
    29941 tattttctaa atatttttcc catgcttcgt caaagatatc catggacggc aaatagtcga
    30001 cattcagttc cagataatta gaaatttctt cgtagctaga gctgtgctta ggaaattgga
    30061 tgtctttacc ggcttctgta gcgaaaacag ataaagcatc atgtgtgcca ccgcctcgca
    30121 gtgtcattaa ataatgaaaa aagcttcgat tcacccttcg ttcctccaat tttcttcgat
    30181 aaaggcagcc cggcgtgcat ggtttctttt aaaattttca ggatgctttt tataatactc
    30241 ttgatggtct tcttctgcta gataaaatgg ttgggctggt tcgatttttg taacgattgg
    30301 ctgttcgaaa cgtccacttt cagctaactg tttcttactt ttttcagcaa tgatccgctg
    30361 ttcttctgtc aaataaaaga tcaccgggcg ataattgtca ccgcgatcct cgaattgtcc
    30421 gaaagcgtca gttggatcag tttgctgcca gtaaagttct actaattgtt catatgaaat
    30481 caatgttgga tcatagataa tctccactgc ttctgtatgt ccagtcgttt ggctttttac
    30541 ttgttcataa gtcggatctg gtacatgccc tccagtatat cctgacatca ctgtatggat
    30601 accaggaagc gtgtcgaaag gctggatcat acaccagaag cagcctcctg caaatattgc
    30661 tcgttccatt atgattcccc ctctaatact actcatccga tgatttttta ttttcttttg
    30721 tcttgctttc agtactgctg gtgctagttt tgttggtatt tgagttgttt gaagcatcag
    30781 aggtatcttt tggcaaatat agactaaacc gaatgtcatc atttactaag tcgattttat
    30841 ctgctttaat atacatcccg ttttgcattc ggaattgatc caacctaagc atgatagtca
    30901 gatcatctgt attgacttcg atccaatctg gtaatttata gtttctcttg atcatattca
    30961 tcacatcttt gattggcaaa ctcaatgttc cgatagaaag acttctagct tttagttgga
    31021 catttccgtt ttccatcaca tacggatcaa aatataaata gaaatcgata ggaaaaccta
    31081 gtacttcgaa agtaccattg agtagtgctt cattttccaa ataaaattta tattcaatat
    31141 cactatcttt ttgatactca ctaaggaaaa agctgatgat ttgattgact ttttctttat
    31201 ttgaattgat cgttaagaca ggatctcctt gtctttccgt aagagctggt atttctggga
    31261 gttctggttc ccgattagca aaaatacggc tgcctaaaaa tacggtcgaa ccaagtacta
    31321 ccccaagaag gataaggaag gcgaatttcc agtaatttgt tttctttgtt ttgtttgaag
    31381 atacgtcaga tcttgttttc atgtttattg atcatcctcc ttctctaacc attggttttg
    31441 tgtctcgatc agctgatctc tcactgcatt tgccatcaat tgataaccta aattatttgg
    31501 atggaaatga tcttcttcat agagcacatt atttttgatg ccatttagat tttctgtcgt
    31561 gctagttgtt tgatctgttc cttcttctgt gacacctact tcctgattca atccttgata
    31621 aagcaaatca ttgattggga taaagtagca gttcggcatt ttttttgtca ccgtttctgt
    31681 tcctttgttc caattgttaa caattgtctg catgtcagtg atctcaggaa agttcaaata
    31741 aaatggattg tagatgccta aaatgtaaat aggtgcggtt ttattgaggg atcgaaattc
    31801 ttccaatagt tctgtgacat tttcctgata gtttttcagt ggctttttaa aggaattgac
    31861 cgttaacccg aataaattat tttggataac tttcatcaaa tcattaccgc caacagtcag
    31921 tgtgataaaa tcagctgttt tgatattgtt tcgaatttct tcttttgttt ttactcgctt
    31981 taagatctgg tcacttcgtt caccatttac accatagttt tctgtttcaa ccgcggtcaa
    32041 attataacgg tcttttagat catttgcgac aaggggaacg aatcctcctt gcttggtttg
    32101 atctcctact ccttcagtca gcgaatctcc gatagctatg tagtgaagtt tttctttttt
    32161 atttggatct tctctcacag tagttactgg tttcattagt gggccagctt tcggtataga
    32221 agcagctaaa cctgcataga tgatgatcgc aattaataat gggaacaaaa cgaggatcaa
    32281 atgtcgtttc gttaatttca ttttttcacc tcttgtattc gattggttct aatgttatca
    32341 aaaattttat caaaaaggta gtctttatga aaaaagaaaa caattatttt cctaatttta
    32401 taaagaaaaa gagaataagc tgcaagcctt tcaggctgag ggcttatcct cttttcgact
    32461 aatctacata atacatgatt gcaaacgcgt ctttaccagt atgcgtagcg ataaccggat
    32521 tcgtatgcag tacagggata tgcatgtccg ggaataattc ttgcaaaccc tctttgaatt
    32581 cttgcgccaa ttcttttgca tccgcatgtg aaataccgat ttgtcgcaca tttggcaatt
    32641 ctttcagttc atttttcaaa ttattaaacc atttatcgaa cgttttcttt cctcgcccct
    32701 tagttacagg gattaattca gaatgggcga aattcatcac cacgcgtatg ttcaaaacac
    32761 tagacagcaa accagttgtt cggctgattc ttccaccttt taccaagtta tctagtgtgg
    32821 atattccgat gtataatttt gtattgtctt gtactttttt gacttcttca aggattttct
    32881 ctactgtttt accttctttt gctaattcag ctgctcgaat tacctgaaaa gataatcctt
    32941 ggtcagtggt atcactgtcg ataaccgtta ctttcgtttt tgttagatta ctggcttgtc
    33001 ttgctgcttc tactgttcca ctcaagcctt ttgtcatatg gatcgaaatc acttcactgc
    33061 cgtcttctcc tagctcatca tacatcgaga tgaattctcc aattggtggt tggcttgttt
    33121 tgggcagatt ttttgctttc gccatcattt ccataaatct ttcgccgtct agttggtcat
    33181 catcgggata tactgttccg tcgatcatga tggatagcgg catgacacga atgcctaaag
    33241 catcgcgtac atttttttcc atcgtacatg aagaatctgt tacgattttt acatttgtca
    33301 ttattttctc tctctctttc tttaacaaga tgccttattc atgttaaact acatgtaaga
    33361 caatttctat ttttagtata acagacaaat aatgattttt cattaattac acctagtaaa
    33421 ggagccgttt tcatggaaaa aacgaaattt aatcgaacgt atgaaatatt aaatgaagtg
    33481 tttaatgcag tcacccatgg aattgggact ggattgagta tcgcgggact agtggttctt
    33541 ttagtaaaag gtgcccactt gcattcctcc ctccatattg tctcttatgc tatctatgga
    33601 tcgatgatga ttcttttatt tttaacgtct actttgtttc attcattgat ttttacaaaa
    33661 gcaaaaaagg tgttccaagt attcgatcat agttcgattt ttcttttgat tgcaggaagt
    33721 tatactcctt tttgtttgct gagtatccga ggatggctag gttggacttt atttgtatta
    33781 atctggttgt ttgcgattct tggcattata tataaagcgt tgacgttgca taaacgggag
    33841 agagtatcaa aagtatctac gatcatctat atttttatgg ggtggctctg tgtcgtggct
    33901 gctgttcctc tttatcactc tcttggaatg actggtgtta ttttgatggt agcaggcggg
    33961 ctttcttaca cgataggcgc attcttttat agcttgaatt ctatccgcta tatgcatgtt
    34021 gtttggcatt tgtttgttat gttaggtgca ggttttatgt acttctctgt tttattcttt
    34081 acttaataaa aaaaataaag ctatggatgg gacagctcta aataacagct agtctatcca
    34141 tagctttttt atatcgtcaa ttcgcctgtc ccgcttttta caggattctc attacgctga
    34201 tacgtttcaa attgatgcgg ataacgattt ttttcatcca tttctccttg actagtattg
    34261 atcagtgacc aatcagacca gtcaacaggt ggaaaataag catctccatc gaatacatga
    34321 tggatgactg tacggtataa tatcgtacaa aatggcagaa attcttgata aacagctgac
    34381 cctccagcga tgaaaaagat accgtctgtt tctttttcca gagcaagcac gtcttccaca
    34441 ctatgcatga catgcacgcc ttctgcttta taggaagaat ctctcgtcaa aacaatcgtc
    34501 tgacgattcg gcaacggacg gctacccatc ccttcgaatg tttttcgtcc catcactagt
    34561 gtatttgctt ctgtcatttg tttgaagaat ttcagatcat ttggcaaacg ccaaggaaga
    34621 cggtcttctt ttccaatcaa tccgttttca tcctgtgccc aaattgcagc taacattttc
    34681 tcatcctttc tgattctaaa cagcgatagg agctttgatc gccggatgtg ggtcatatcc
    34741 ttctaattgg atatcttcca tctcaaaatc aaatactgac tgcttttcct gattcaaaac
    34801 taaagtcgga aatgcgcgta tttctcggct caattgttct ttcatttgtt cgatatgatt
    34861 gatataaaga tgagcatctc ctaatgtgtg aacaaattcg ccaaccgcta aacctgtttc
    34921 atgtgcaatt aggtgagtca gcaacgcata acttgcaatg ttgaacggaa cgcctagaaa
    34981 cacatcccca ctgcgttgat acaattggca gcttagccgt ccctcttgaa cataaaattg
    35041 aaacatcgta tgacaaggag gcaaagccat gctaggaaca tcttccggat tccatgcaga
    35101 aacgatcaat cttctagaat caggagtctt tttgattgct tcgatcactt gtttgatttg
    35161 atcgataaag ccaccgtctt ttgtttccca gtgacgccat tgatagccgt agatgtgtcc
    35221 aagatcgccg tatgtttcag caaaggaatc atcggttagg atcttctcgc aaaaaagctt
    35281 gctctgcttt ttatattctt cattgaattc aggatctgtc aaacatctat ggccaaaatc
    35341 agtcatatct ggtccattat agtctggact tttgatgtag cgttcaaatg cccattcatc
    35401 ccaaatatgg tttttatttt gtaatagata gcggatattc gtatctcctt ttaaaaacca
    35461 aagcaattcg cttttgatca atccaaaagg tactcgtttc gttgttaaca aaggaaaccc
    35521 tttttgcaga tcaaaccgca tttggtatcc gaataagctt aatgtgcctg tgcctgttct
    35581 gtcttcttta cgatgccctt cttccaacaa ttttcttcct aagtcaagat acgcttgttc
    35641 catccttttt cctcctaatc ttctacccat tcactcaagt attcccaccg tgccattttt
    35701 tcttcaagtt gtgcttctgt ttcagatacg tcgttttgca attcttgtaa tcgagtgaaa
    35761 ttatctcctt gatgattcat ctcttcttgg agcaatgaga ttttttcttc aagttctgca
    35821 atctcatctt caatcgtttc ccattctttt tgttccatgt aagataattt cttcttttca
    35881 gcaggttcct tgctctgtat ttttggtttc gctttttcag caggctgctg ggtctctttt
    35941 tgcttcagta aataactgga catgtttccg taataaagtt cttgttttcc gtttccttgg
    36001 aatactaata acttatctgc taccttgtcc aagaaataac ggtcatggga aacagtgatc
    36061 acagcacctg gaaaacttct aaaataatct tccaaaatcg tcaatgttgc tatgtccaaa
    36121 tcattggtcg gttcatccaa taataaaaca ttaggttgct gaatcagcag tttcaacaaa
    36181 aataaccggc gtttttctcc gcctgaaagt ttgcctatca gtgtcccatg cataaatctt
    36241 ggaaataaaa atcgttcaag taattctgct actccgattt gactgccatc tgctgttttt
    36301 gcttcttcag cagcttcttg taagtaagca atcatccgtt tatctggtgc catttcttca
    36361 ttttcttgtg tataatacgc aagatgaacc gtttcaccga tagacattgt accgctatcc
    36421 aggggaattc tgccagctag aatattaagc aatgtcgatt ttcccgcacc atttttacca
    36481 gtaataccta gtctttcccg agattgaatc aataaatcta aatgttctag cagtgtttgg
    36541 tcatttatac ggtaactgcc atctttgatt tccagtactt ttttccctaa acgttgtgtc
    36601 gctaggttca gctctaaatc atcttcttgg tttacttggt acaagttctc ctttagatct
    36661 tcgaaacggt tgatccgtgc ttgctgcttg gtgccacgtg cctgtacgcc agccctcatc
    36721 caagctaatt cctgtttata aagccgcttg cgcttttctt cttgttctac ttctacacga
    36781 tcacgttctg ctttttctaa gacgtaagct tcataatttc ctttatactc gtataatttt
    36841 ccaaatgaaa gttcgaagat tcggtttgct actctatcta gaaaataacg atcgtgggta
    36901 accatcaata gtgcgccacg gtattgtttc aaatagtttt ccaaccactc gatcgcttca
    36961 taatctaaat ggtttgttgg ttcgtctagt aatagtaaat cgggttcatc aatcaacact
    37021 tgcgccagac taactcgttt tttctgacca cctgataaag tactgatttt tttatctaat
    37081 tcactgattc ctaatttttg caaaataatc tttgcgttgg tatctgtagt ccaagcatct
    37141 tctttattca tttgttcttc tgctttagca taacgttttt gcacatcagg gtcttctccg
    37201 ttttccccta gttcgatcaa tgcttcctca tatgctcgaa cagtttggat caaggggctg
    37261 tttccttgaa agactgcttg gagaacagtt aattcatcgg aaaattcagc tgcttgagaa
    37321 agataaccaa tacggtaatc agttggataa aacactgttt gccgatcacc gtcgctgcta
    37381 tcgataccgg caataatatt taagagactt gttttacccg tcccgttcgt cccaatcaac
    37441 ccgatacggt ctttttcgtg gaccaaaaaa gaaatctggt caaacaaatc tttttcacca
    37501 taagtttttt tgaggtcagt tacttttaat tctttcaaca tgttccctcc ttatcttgga
    37561 tattgtatca taaaaatagt gaacttcagg aaaatttttt tggtatttgt tctttttgtg
    37621 ttctcaaaaa aagaacagga ccgattcgat cctgttctga tatgttttgt tttttataaa
    37681 acttgaaaat ttttaccttt ttattttatt ggactcgttt tttttgactt tgctcgaaac
    37741 gcaaatcgga aagaaaagtt atcagatggt ttgttcatcg tataagattc taacaaattg
    37801 atattcctgc gatagctgtt cgcagaaaac gtcgtgattt tttcagcaac ttcgtattca
    37861 tcaatgattt ttctttcaag ataataacct cttagcattt ttttcgtaaa cagactatct
    37921 tgctggatca tccacgaaga aatcaaattc cccgagttca tttttattcc ttcaagtttg
    37981 cgttgctgga taaaaaattg aaccatcgtt tcatcataaa tatcagtcaa gttatctaaa
    38041 ctttcaataa tcaactgagt atttttttga tatgtctctc tgatttcttg gatatcttca
    38101 tcactaataa atgtctgacg atcagcatat cttctttgcc aaaacatttt cggatggaga
    38161 acgattcgca ttaacctacg gacaacaatg aaccaaaagc cgataaaaga caaaatctgg
    38221 cttgtgatag aatgttcgaa tcgagaaaga aaacgggagt aaaaacgata accattagaa
    38281 gaaagcttgc ccgaacgata actttcatcc aatccatccc gttcgatcga caaaatcagt
    38341 gcttgtattt cttgtacttc ttgcttttca tcatcggtta aatcttccaa gtacagatca
    38401 cgtatccgtt cttgataatt ttctatcact gcttcagtgg ctaaaaattc ttttgtatta
    38461 cgatcaattt cctttatttc ttttcgcaaa acttcaacga cctcttctaa aatcatcaaa
    38521 gcattcaaat ccgtgctttt cgcttcctct gtttccgtca gcataggcaa gacaatgatc
    38581 cctatgacca aagtcactaa gatgacaccc gccgtcagaa acaaaagcaa agaccgctgg
    38641 taaaacatca tattattgat tgaaaatggc aaaataaaaa tcgtagccaa actgactgtt
    38701 ccctttacac cgccaaatgt taagatcaag atctcgttca actgtttttt gaacttttta
    38761 ctgccatctt tgaatacata aaaaagactg atagaaatga atctgatcac aaaaagcatg
    38821 acagagatta atacgataat cgctaataaa agaccgtttg gatataaacc gtctccccat
    38881 actggcgaga acacttgcgt caattcgatt cctaaaaata taaatactaa tgcattcagt
    38941 gtaaaagcaa tagttgtcca tgtactgtgg gacaagctcg acaattctgc atcaaacaca
    39001 gagattttcc taaaaccaga agcttggaga atacctgctg ccactgcagc aatgattcca
    39061 gatacatcaa atgcctctgc caatacatag gctagaaaag gaagcaaaag ttcgatcagc
    39121 agataccctg tgacatcttg tgctgatgct ttttcgatca aatgaattat tttttgtttg
    39181 aaccagacaa gcaaaaagcc gatcaaagct cccccaatac tagaaactac tagactcatc
    39241 cccgcgttta ctgctgaaaa agatccggtg atcagcgcac caagagcaaa ctggaaggca
    39301 gtaacaccag atgcatcatt caaaagacct tctccttcca aaatatgcat cgccttttta
    39361 gggatattca ctcgtccaga aagagaactg actgctaccg catctgtcgg acctaacgct
    39421 gctccaaaag caaagcaggc agcaagtggg acacttggca acaggaatga taacgtcgca
    39481 cctaccccga caagggtcaa aatgactcct ccaaatgcca agaacaaaat tgtcccgaaa
    39541 tttttgagaa tcgaagaaat atctgctttt tctccttctc taaataacaa tggtgcaata
    39601 atcataacta agaacaattc aggttcaaaa tcgattgatt gtccccactc tgtcagacct
    39661 aataccactc caaggaaaat ttggatcagt ggagctggaa tcattggcaa aatgcggcta
    39721 gctatgttcg ataaggtgat cgttactgca aatacaataa ctaactcagc aaattccatc
    39781 gtccatcccc tttatttttt attttgtgta agcattttta aaaattgttc ttttttcttt
    39841 aaaattttgg tttcgactac ttcaacctgt gtttggttcc cctctaattc acactgtaca
    39901 cgttttgact ccaaacgaag cagatcatgt ttcagacgtt ccaactgacg atacatcaat
    39961 atatttcttt tacgcaactt acaaagtatt tgacggcggt catccatcga aagggcgcca
    40021 tagcgatcat gcaacagttt tttttgttgt tcaaaactct ctctcatttt ccagatctcc
    40081 tatacacaaa cttccatcat tatagcaaag ttctttttgg atgaaaaata tttcattgtt
    40141 tcatcgttta ttgctagtta gaaatttcca taaatttttc aacgcctcat tctattccaa
    40201 atagtatcag atatcaagta aaacttaaga aagataaaag tttctgacaa aaggatgttt
    40261 actttcccat tcctgccttt cttgttcttt tatcacaaca aaaaaatggg gctgcgacaa
    40321 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
    40381 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
    40441 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
    40501 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
    40561 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
    40621 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
    40681 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
    40741 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
    40801 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
    40861 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
    40921 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
    40981 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
    41041 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
    41101 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
    41161 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
    41221 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
    41281 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
    41341 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
    41401 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
    41461 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
    41521 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
    41581 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
    41641 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
    41701 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
    41761 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
    41821 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
    41881 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
    41941 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
    42001 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
    42061 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
    42121 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
    42181 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
    42241 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
    42301 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
    42361 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
    42421 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
    42481 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
    42541 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
    42601 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
    42661 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
    42721 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
    42781 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
    42841 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
    42901 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
    42961 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
    43021 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
    43081 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
    43141 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
    43201 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
    43261 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
    43321 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
    43381 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
    43441 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
    43501 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
    43561 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
    43621 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
    43681 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
    43741 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
    43801 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
    43861 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
    43921 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
    43981 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
    44041 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
    44101 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
    44161 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
    44221 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
    44281 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
    44341 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
    44401 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
    44461 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
    44521 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
    44581 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
    44641 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
    44701 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
    44761 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
    44821 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
    44881 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
    44941 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
    45001 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
    45061 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
    45121 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
    45181 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
    45241 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
    45301 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
    45361 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
    45421 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
    45481 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
    45541 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
    45601 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
    45661 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
    45721 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
    45781 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
    45841 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
    45901 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
    45961 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
    46021 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
    46081 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
    46141 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
    46201 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
    46261 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
    46321 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
    46381 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
    46441 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
    46501 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
    46561 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
    46621 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
    46681 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
    46741 gaaaagataa gagcaaagct cacctcataa cttgcaaata aataaaaatt aaataaaata
    46801 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
    46861 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
    46921 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
    46981 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
    47041 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
    47101 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
    47161 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
    47221 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
    47281 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
    47341 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
    47401 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
    47461 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
    47521 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
    47581 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
    47641 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
    47701 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
    47761 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
    47821 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
    47881 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
    47941 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
    48001 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
    48061 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
    48121 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
    48181 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
    48241 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
    48301 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
    48361 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
    48421 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
    48481 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
    48541 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
    48601 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
    48661 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
    48721 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
    48781 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
    48841 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
    48901 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
    48961 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
    49021 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
    49081 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
    49141 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
    49201 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
    49261 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
    49321 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
    49381 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
    49441 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
    49501 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
    49561 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
    49621 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
    49681 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
    49741 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
    49801 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
    49861 acggcgtttt gaaagtagat agctgccgtt ttctttatct ttgccgatcg tactgataac
    49921 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
    49981 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
    50041 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
    50101 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
    50161 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
    50221 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
    50281 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    50341 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    50401 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    50461 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    50521 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    50581 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    50641 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    50701 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    50761 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    50821 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    50881 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    50941 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    51001 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    51061 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    51121 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    51181 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    51241 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    51301 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    51361 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    51421 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    51481 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    51541 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    51601 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    51661 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    51721 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt ccccaatgaa
    51781 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    51841 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    51901 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    51961 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    52021 gctccatcca ttttttttgc agctcatcaa agggagcttg ctctgtggca tattccaaat
    52081 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    52141 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    52201 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    52261 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    52321 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    52381 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    52441 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    52501 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    52561 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    52621 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    52681 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    52741 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    52801 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    52861 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    52921 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    52981 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    53041 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    53101 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    53161 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    53221 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    53281 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    53341 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    53401 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    53461 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    53521 ctttgttgca gataaaccgc tgactcatca ttgttttttg ccagctgata aggaagcagc
    53581 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    53641 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    53701 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    53761 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    53821 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    53881 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    53941 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    54001 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    54061 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    54121 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    54181 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    54241 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    54301 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    54361 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    54421 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    54481 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    54541 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    54601 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    54661 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    54721 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    54781 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    54841 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    54901 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    54961 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    55021 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    55081 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    55141 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    55201 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    55261 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    55321 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    55381 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    55441 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    55501 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    55561 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    55621 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    55681 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    55741 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    55801 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    55861 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    55921 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    55981 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    56041 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    56101 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    56161 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    56221 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    56281 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    56341 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    56401 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    56461 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    56521 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    56581 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    56641 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    56701 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    56761 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    56821 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    56881 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    56941 atggagctat tgacttatcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    57001 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    57061 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    57121 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    57181 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    57241 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    57301 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    57361 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    57421 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    57481 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    57541 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    57601 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    57661 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    57721 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    57781 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    57841 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    57901 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    57961 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    58021 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    58081 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    58141 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    58201 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    58261 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    58321 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    58381 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    58441 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    58501 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    58561 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    58621 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    58681 tttccctatt ccctcctctt ttttcaggtt tttaaagctt tcttcttttt cattgtagcg
    58741 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    58801 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    58861 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    58921 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    58981 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    59041 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    59101 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    59161 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    59221 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    59281 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    59341 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    59401 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    59461 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    59521 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    59581 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    59641 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    59701 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    59761 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    59821 ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa gtgttcattt
    59881 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    59941 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    60001 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    60061 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    60121 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    60181 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    60241 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    60301 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    60361 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    60421 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    60481 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    60541 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    60601 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    60661 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    60721 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    60781 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    60841 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    60901 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    60961 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    61021 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    61081 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    61141 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    61201 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    61261 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    61321 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    61381 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    61441 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    61501 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    61561 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    61621 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtga cgaattcttg
    61681 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    61741 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    61801 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    61861 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    61921 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    61981 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    62041 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    62101 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    62161 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    62221 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    62281 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    62341 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    62401 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    62461 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    62521 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    62581 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    62641 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    62701 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    62761 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    62821 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    62881 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    62941 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    63001 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    63061 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    63121 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    63181 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    63241 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    63301 ggtttctttt ttctttttca tagcattcct gacagattcc atggaacgtt agccgatgat
    63361 cttttactaa aaaatgataa cgttcttcta ctacttgctc cacttcaagt aaaagatcct
    63421 cttctacttc ctcgatcgtg ccgcagttca agcatagtaa atgatgatgg aaatgtttgg
    63481 cgccttcttt tctcaaatca taacgggcta atccatcatt aaaacttact ttatcgacaa
    63541 ttttcaattc tgtcaagatt tcaagggttc gataaacagt agcaagtcca atttccggac
    63601 tctttttttt cactaaaaag aagatttctt ctgcagataa atgctctttc tcattttcca
    63661 aaagcacaag cagagtagct tctctttgcg gtgtcagctt aaaccccgct tcatgtagct
    63721 gctgttttgt ttttttcatc gccgaaatag cattcatgac catttactca accccaacaa
    63781 tttataataa ttacaaataa aaaaagatat aacactatgt taattaaaat tattataagc
    63841 tagttaagaa aaacatgcaa gggattctca attagatttt ttgactagtt ttgtcacacc
    63901 gttttcttgt gtaatcaatc cttgtttcaa aagattacct aatgcgcgct taaatgctcc
    63961 tttgctgata ccaaaagctt tcatgatttc ttctggatcc gatttatctg taaagttgat
    64021 ttgctgatct gcagaacgtt ctaaaatggt taaaatcatt tgtgcatcat ctgatatagc
    64081 ttcatatgct cttggcttca atgaaagatt cagtacaccg tctggtcgga caccaatcac
    64141 tcgacctttt atctgctcgc ctagacgagg ttcattatat cgttcagaag gatggataaa
    64201 cccaagatag aaatcttccg ttaaaacgta cgtacctgtt aatttcaagc ggtatgctgt
    64261 tcctatcaca tctttgtttt tcatttcttc tgttccatat ttgcttaaag atttgaatat
    64321 tttttcatct gctaaactgc cccaaatacg gtcttttgca tcaactctaa gggaaatcat
    64381 caagcgatcc ccttttttag gccatagctc tgtcattgtc ggcaattcat caagagatac
    64441 gacaaagtct ttatctggaa gaccgatatc gacaaacgca cctaaatctt ttcgtgtagc
    64501 agtaacgatt ccaaaagcgt aatgaccgat acggctttta ggaatttgtg tagtgatccg
    64561 attttcatgt ttttgatttt ggtaaccgaa tccttcaact gcttcaccga ttttgtgttc
    64621 accttctgat ttgcttaagc caaaggtttg tccgtttttt tggacaaagt agtttttttc
    64681 attttcatca ataataatac ctgtaaatat agttgctaat aaatcgttca tttttttctc
    64741 cctctatagt gaaaggtttt cttaatacat ttctttgcta tcataacata atgttatcta
    64801 cttgaatagc tgagccttgg tttggaaagg tgaaaaaagg agtatgacaa cagttgtgtc
    64861 atactcccct ttttcgaata aacggtgtta aacggcaggc tcacagcctc tttcatttgc
    64921 ctaaaattaa ttgctcccat ttcctccgaa ccatccagtt ttacagttga tggttacgat
    64981 gtaaaatggc aaagcacttt ctccaatgcc tttcatcaat gcagattttg ttgaagtggc
    65041 atatagatta taatagcctt ccccattttc gattctggct ttttctaatc tgtaacctcc
    65101 ttcaacgata tatccacgtt ggatacatgt agcaatcact ttgctttgga cacctggtgc
    65161 ccacgtccat gctggattag atgcatcggc aatatcactt gctctggcag atttgattgg
    65221 ctgattcgct gagtttgaag aggaaggggt cgaggatgcc gcttcttgct gtgctgcttg
    65281 tgcgaccgca gcttgttgag cggcttgagc tgcagctgct gcttgagcgg cttgttttgc
    65341 ttgttcttca gctgcttttt tcgcttcttc tgcttttttc ttttcctctt cttctttttt
    65401 ctctttatca gtgagcatct tatctacttg tttcaattgt tcattcaact gagcagatag
    65461 atctgtattt ttcacttttt ctactgcttc ttttgccgtc ttgtattggt cgcgtgttgc
    65521 tgaatccact acttttccgt ctttaaagac tatagctgtt ttttctttag ctgtttgcaa
    65581 ttcagcatat tgatttttag cttctgtttg tgcttgttga atcaattttc caaaatctgt
    65641 gttatccgtt tcgattgctt ggatctcttt atctgctttc aacttgatgt catctgctag
    65701 atgatcatcg acgatggcag gtgctatgaa ccactcattc actttttgga tatctcggat
    65761 cttagattgg atatcttggt aagttgtttc taactctgaa tatccttttt cattcttaag
    65821 actggataaa tcatttttta gttttgataa atcttgatga atcatatctg cacgaataaa
    65881 ttgatggtga tcatctgtat aaaaggcagc aagtttctca tgaagttctt ccatttgttg
    65941 ctgtcttttt gcttctgctg ctacttcggc ttctactttt tgatgatggt cataataagc
    66001 ccagcttcct ccaccgagta gaagtactgc tgctaaaacg agataaggta ctttttttga
    66061 ttttttcttt tgcgctttat ctggatctgt tttttctaag tttgattctt gatgtgtttt
    66121 ttcagtatct ggcacgattg ttattgattt cgcttctgct tcaaacagct taggctggtt
    66181 ctcttctttt tctatttttg cttcaagatc agtaccctct ttgcttacag attgattttc
    66241 agcatcggat tcactattta tttcatcggg ctctgttgac tttttgtctg tgttttcgag
    66301 tatttctttt ttctctgctt ccggcagttt attcgttttt tccttttcta attgtttttc
    66361 agtaaatact tcagtttttt ctgtgatatc cgtttttacg tcttttattt ttactgtttc
    66421 tttacctgtg gcgttttctc tatcagagtc tttttcagtt tcttgttcct tttctggctc
    66481 tactttgttt tcctgtgcaa ttttttcact gatcattttg agcagctctt tcgcgtgctc
    66541 ttcttttgta tcttcacgat gtgctttgat ataagcggat agtatcgaat tttcttcctc
    66601 attatcgacg atttctttca gttcagctac tcttcttttt tgttttgatt caagcgtttc
    66661 ttcttgattc tttcctgatt cttccgaagt ctcatgaaca gttgctttct tctgttcatt
    66721 tttctctata ttagttactt gatcgtgagc aggtggttct tcgtcttctg gttctgcttt
    66781 ttcgaataat tccattacag attctagagg taaatctttc aattccgacc attcgattgg
    66841 atcgtttaat tctgtatcag gaaagactgc atcttcagtt gatttatcct gcatagctgc
    66901 atctgtattt gtttcttcaa ttgattcttt tatttgctga tcttctgcag ttatttgatg
    66961 accgcagtct ggacagatag tattctgctc catttttttc gatccgcaat ttggacattt
    67021 tttcatcact tagcgatcgg ctcctttact aggtttacgc taccatttaa tctatcatat
    67081 tctatacgtc acgccaatag cttattttta ggatttactt aaaaataatg aatgaatttc
    67141 cttcctatag aaaaaaaagc ttgagactgc gtagtctcaa gctttttttc ttttaaaata
    67201 aaccaaacag ttttactgat ttctcttctg gtcaatagtc cacgcttatt ctaagaaatc
    67261 ttttaattgt tttgaacgag aaggatggcg caattttctt aatgctttag cctcgatctg
    67321 acgaatacgt tcccgagtaa caccaaatac tttccctact tcttccaatg ttctcgtccg
    67381 gccatcatct aaaccaaaac gtaagcgaag gacattctct tcccgatcag tcaatgtatc
    67441 tagtacatct tccaattgtt cttttagtaa ctcataagct gcgtgttctg ctggacttgt
    67501 tgcttcctgg tcttctatga aatcacctaa gtgagagtca tcttcttcac cgattggcgt
    67561 ttcaagggaa acaggttctt gcgcgatttt caagatttct cggacttttt ctgttggaag
    67621 atccatttcc gcaccgattt cttctggcgt aggttctctt ccaaggtctt gcagcaattg
    67681 acgttggata cgtatcaatt tattaattgt ttcaaccata tgaactggaa tacggatcgt
    67741 acgtgcttgg tcagcgattg ctcgagtgat ggcttgacga atccaccatg tagcataggt
    67801 tgaaaactta aatcctttgc ggtagtcaaa tttttcaact gccttcatca gtcccatgtt
    67861 tccttcttgg atcaagtcaa ggaactgcat gcctcgtcct acataacgtt tagcgatact
    67921 tactactaaa cgaaggtttg cttctgccag acgctgtttc gcttcttggt cgccttcttc
    67981 gattttcaat gctaacgcta cttcttcttc cgcagttaaa agggagacac ggccaatttc
    68041 tttcagatac atacgaaccg ggtcattgat ttttacaccg gttggcgcag aaagatcttc
    68101 tgtttttgct tgttcagctt ttttctcagc actcttcaag ctgtgaattg atggatcacc
    68161 attttcatcc accacactga tacctgcgtc ttcaatcttt tggatcaatt tgtccatttc
    68221 atctgcattc aaagtgaatg gtgtagcaag ctgattggat aaatcatcat atacaacttg
    68281 tcgtttgggt ttgttttctt tgatgaaagc tgctactgct gcttcatacg tttttttatc
    68341 tgttccattt gccataaaag aaggcccccc ttgagtagct atttggcttg ttttaactgt
    68401 ttcgttaaat tgatgatttc aatcgtcaat tcaagctcaa gctgtttgtt tccagttcgt
    68461 ctggcttctt gctgcatttg tttttttaat gttatttcat cagctaagcc agatttgctg
    68521 atcacatcta gcaagtcttg gatttctcgt ggcgaacttt cttctgccac cggtaaatat
    68581 tcgatttcca ccaccaaccg ccttaaattt tcatcttgca aaaagtctaa aaactctgca
    68641 aggttgaatt cataatgttg tgagacatat gcatcgaata aaaaataaac ttctgcatat
    68701 gcatcatgaa taaactgaaa ttctcgttct tttaaaatat tacggacaga aacttcttta
    68761 aatgcacgat ataatagaat ctgctctgcc ttttctactt gcgtcaaagg tctctttttc
    68821 cgtgcagttt cttgtctcgt aacctgagtg tgttgacttt cctgaggttc ttgacgattt
    68881 tgctgatttg tccttcgaag atcgcgcagt tgtttttgta tcgtctctct gccgatctga
    68941 aactctgttg acagttgaga tagatacata tcttgttcga ttggtgaaac aactcggcta
    69001 agttcaacta gtaattcttc gaggtattcg atcttgtctt tttcattatc taaatttttc
    69061 ccttgtttta aataacgtga tttaaaagta aagactgttt cgcgcccatg agccactaat
    69121 tcttggaaag atgcttctcc atacttacgg agatattcat caggatcaag cttttctgga
    69181 acgactacga cagaaagtgc taatcgactg ttttgccgaa gcaattcaat ccctcgattg
    69241 gttgcttcaa aacctgcgtt gtctccgtca tagcaaaaga ccaattcttt cgtcagcttt
    69301 tccatttgtt ggatctgttg agctgttaga cttgtcccca tagaagctaa tccagttttg
    69361 ataccagcct gccatgcagc aatcacatcc ataaaccctt cgaacaacaa aatttctccg
    69421 tttttccgga tatcgcttct tgctttatca agattaaata aaacttgtct tttattgaac
    69481 agttctgttt ctggactatt caagtatttg ggctgatctt tttcctgccc tttttcaggt
    69541 gcaagccagc gtcctgaaaa accgatcgtc tggccttgag aattacgaag tggaaacatc
    69601 actctttgat aaaaacgatc agacatacgg ccatcatcat gcgtgacaaa caatccagag
    69661 cgttctaaca attcatttga aattgattca ttcttgaata cttgctgaag aaaagaacgc
    69721 tcattaggtg caaaacctat gttgaattct tcaattaatt ctaaagacaa cccacgactt
    69781 tgcaaatatt ctaaagctgg ctgtccagct tttgtatgca atagcatgtg atgatagatt
    69841 tccgctgctt tttcatgagc agcaatcagt ttccccgtag cagattcagg atttcggaca
    69901 gctatagtac gctgacgcca ttgatcttct aacggtatct gttcgaagtc tgccactttt
    69961 accactgctt ctggaaaact tagaccttct agttcttgaa gaaaagtaaa cacgtttccg
    70021 cctttaccac aaccaaagca atgaaaaatc tgcttgtctt ctgcaacaga aaaagacggt
    70081 gtacgttctt catgaaaagg acacagcccc aagtaatttt taccagactt tttcaactgg
    70141 acatattgtc caatcacctc aacgatattc gtttgactgc gtactgtttc gataacctct
    70201 tgaggaatcc gttgagccat tttttcaccc cctgctctat tgatatcttt tgggtttaac
    70261 agaagatatt tcaggagaat aaccggtaaa aaatcgcact aactgaaatc agtgcgattt
    70321 taaaggacac aattatacat gtttgattgt atcatatttc attgcttttt caagtataac
    70381 acatttctat ctcattaagc atcaatttgc tactcttttc agtaactgat tttgaatatc
    70441 agccttccag tacaatactc gaagaacaat atttacgagc atgaatacaa agaagtatcc
    70501 tacgctctct cccaacccta agtttacttc caaaattcca tggaacagca ttgcagctac
    70561 atatatccct acgatcgtcg ttactgctag gaagatgcgc aatgggttga agggataaca
    70621 agcttttaca actgccatgc aactgatccc aatcagcaag taatacatca aagtagtagt
    70681 ttcagcattt ccccatgcga atgtttttcc aagtagatac accgcaatga tattcactac
    70741 tacaagcaag gcatttggta aagcattaat aagtgctgta ggcaaataac ggtaagtgat
    70801 ttttctttta tcttgttcaa aagataaaaa gaaggaagga tacccttcaa tcgctaaatc
    70861 aatcagcgtg atttggatag ggataaacgg aaatccaatt gctgtaacgg cacatataat
    70921 cgataaaata aatgaataaa tcgttttgat aaaaaagatg cctgagactt tcgtcacatt
    70981 gttgaccact cggcgtcctt caaacaggac ttctggcaat gtagtaaaat cagagtccaa
    71041 caataccaga ttcgcaatct gacgtgttgc tccatcacct tctgccatag caatgctgca
    71101 atctgcttct cttaacgcca aaacatcatt cacaccgtcg cctgtcattg caaccgttct
    71161 gccgctttct ttcaattcat ttaccagcaa tttcttttgc tgcggactca ctcgtccaaa
    71221 aaccgtatag cgatgagcag cttctcgcac ttccatttct tcagtaattt gagaaagatc
    71281 gacatacgca tcataattcg gtaatcctgc acgtctagcg atattggaga cagtaaaagg
    71341 attgtctcca gaaatcactt tcagatccac accttcctca tgaagataag ctagtgtttc
    71401 ttgagcgttt tttcggattg gatcatcgat ttcaaacaga gcaaccggct caacaaatgc
    71461 tggtttttct tcgttcaatt gctgatttgg agcgatcccc aacataagta cgcgatagcc
    71521 attcgcttgg gctgttttta gactgtccgg aagccgatct tcttccacca aacgttcagg
    71581 cgctcctacg tacacgactc ccagttctgg aaattccatc gcgccccatt tgcgatcaga
    71641 agaaaatgcg actttgtttg tcacatcata ccgattagaa agctcaaaat aatctctcat
    71701 agcccgcata gtgatgttgt tatcactgct ttctgccaga tacgtcccca taatttctgg
    71761 gatggctttc tcgtacagcg gatgtagtgc ttctacccgc tggatactca ttttcccttc
    71821 agtaatggtc cctgttttat ccaagcataa tgtatcgaca tgggccagcg tttcaattga
    71881 atacatgtct tgaacaagga ttcgtttttt tgccagtttg gttacccctg tcgttaatgc
    71941 aatacttatt agcaatacca aaccctttgg aagcatgcct aacaaagctg ctgcagatgc
    72001 aataacagaa attttgatag aagcatcgcg gatgaacagt gcttccaaaa atagtatgat
    72061 ccccaaagga ataatcacga aactagtgaa tttggatact ttgcgaatcg aatctacaag
    72121 ttcagaacga atagctttgt gagtctttgc ctcgctagtc aagcggacag cataattatc
    72181 tgcacctact cggataactt cagccatgac agaaccgctt gtcaaaaagc ttccagaaag
    72241 caattcagct ccagtttctt tgacaatcaa atcagattcg cctgtcaaaa gagcttcatt
    72301 tgcttctgcc cgtccagata taacctgtaa atcggctggt atctgttctc ctgctccgat
    72361 ttttaccaaa tctcctaata ccagctcttt agtagaaatc gattgttctt taccgtcccg
    72421 gatcactagt acttctgatt gggaaacaat cgataatttc cgtacaagat ttcttgcatg
    72481 tatttcttga taaattccaa taacgatatt gacgacaata attgctaaat acgccatgtt
    72541 ggaatacgca cctacaaata acagacaaat cccaatacca aagttcagaa aattgaataa
    72601 cgtcaatata ttatcgctga atatttgttt tgtacttttt gatgcatctt cctggtagtc
    72661 attttgctgg ccagaagcca ttttctgaac tacctcttgc tgtgtaagtc cattcatttt
    72721 ctttttctcc attttccgcc aactttcaaa aataatcacg tgtttaagtc tagcgtgttc
    72781 caagattaaa aacaacttgt ttttataaaa tttatagaaa aacacacgaa tgattttaca
    72841 aaaaagatgc caaccttttt gtttgaatca cccgtgtgct gtttttatta tcaatctatc
    72901 tttacttata ttgttcctcc aaacggctca tccaccaacc tagtgctgta ccggctataa
    72961 aaagaacagg acaagctagt accccagcaa ctcccaatcc cgcgtaatcg gtaggaatga
    73021 tcataatcgt tgaagcaaaa actacaccta aaatgaaatg gaatagcttg ctatatgcct
    73081 ttgagaaaat gtaatccatg atttttgaca atccaagaac acaaagaatc ccaccaattc
    73141 cgatcggcac aatcacttca agtcgtactt ctttaaatcc gtcagccatc gctttataca
    73201 atcccatata caccaaaaaa ttagaagggc tcaatcctgg gacgatcata cccaaaccaa
    73261 tcaatccgcc ggcaatcagc catgttccaa aattctgatc gacttgcccg tcaaataatt
    73321 gagcgccgta taataaaaac aaataagcca gaacaaaagt aacaaccatg atgatcacat
    73381 cagaagtatc tcttcctttt ttccctgctt ctttccataa cgcaggaaca gttccagcga
    73441 tacatccgac aaaaaaccat aatatcgttg tttcatacgc acctaataaa aaactgacta
    73501 aaaaagaaag tagaaagatg cctgtcactc cgcctagacc aacaggcaga aaaaacaaaa
    73561 cattttcttt gaaatttttt gtaaggtggg ctaaaaaaga aatcagccgc tcatacatgc
    73621 caaagacagc tgctaatgca ccaccgctga ctcctggcaa gataaatcct gaaccgataa
    73681 acattccctt cacaaaccgt aacatccagt ctttggttgt gtaccccact tcttgatttt
    73741 ccattttttg ccttttttcc actatttgcg tactccttct ttcatttaat tttattcatg
    73801 attgaacatt tcctgctatt ttacttaaag actttttcat aggtctctat tattttttca
    73861 ttcattttga caatatcagg ataaaacatg ttacctccat tatgatagag atagccgcca
    73921 ttgtaaagca aggactggat ccgccaatat cggtattgtt tcccttcctc atttcctaaa
    73981 agtgggctca gcacatcttt agaatacgct tctgctaatt ctactgtgtt ttctccgcca
    74041 tgatttttta cataggcaat ataatctttc ccaaaattat atgcttggac agctgtccat
    74101 aaatcgcatc cttgtttctt tgcttctact atcatttccg ccaaatattt gacaccttga
    74161 tcaatactgt cagctggatt atcaatgatc tcagcttctc catgcagact ttccgagctc
    74221 tgcatgagat ctgtcccttt tccttttgat tcggtcaata ttatcgcctt gatcaaattc
    74281 tcgtagtttt ccatatgata attttttgca gctgttgtta tttctatttg atagctttcc
    74341 actcgtttta ccgaacgata agttcttcct cataaaaaca acaccgcacc aataaccgtc
    74401 agaatgatca tgtaaaaaag aatacggata atagataatc gttttcgctt catttcatca
    74461 cctagttatt ttgacatctt tttttatctt actatttctt tatcttcttg gctatttctt
    74521 ccttgatttc tgtgtacgcc ttaagaaaaa atgaattttc ctgtattttt tgacatctta
    74581 cgaaaatgat cgttgttttt cagtaaaaac aattgcccaa tgactgatat aatcgttata
    74641 ctttacacag taggtgatta tgtgaaaaaa gaaaagaaat atgcaaaaat gccagatggg
    74701 agcgaaattt actatgaaaa gagcggtcaa ggcttccctc tctttttact gcatggcaat
    74761 gacggcagcg gccgcttttt ttcagaacag gtccctgtac tcgaacgcta ctataccgtg
    74821 tatctagtag atagccgagg acatggtcgt tcgacaaacg aagcatctat gttgaatttt
    74881 cagttaatgg ccgaggatct gaatacgatc atgttgctgg aaaaaatcga tcaggcagat
    74941 tttcttggat ttagtgatgg agcaaatcta gcactggtat ttgccagcag cttccctaaa
    75001 aaagttcatc gattgatttt aaattctgga aacacgcttg ttaaaggcgt tcgtttttct
    75061 gccagagtca tcagtaatat tcattatgca tgggtatggt tactctcttt atttcgccca
    75121 agtcttcgca aaaatctttt agttatcaaa cttttgctgc atgacatcgg gcttacagaa
    75181 aatgatttaa aaaaaatcaa ctcccccact ttgatcatcg tgggaaaaaa agatgtaatc
    75241 aagctaaaac attctttgta tatagctaaa actataccaa aagcttcctt tgtgcttgtg
    75301 aaagaacaag gacatgaatt agctagaaaa gatcctgaac gcttcaatcg agaagttctt
    75361 caatttcttt ctgaaacata actattagga atagaaggtg atttatttgt taaaaaatac
    75421 catacaatgg ctgaaaaacc acctaggact gttcaaaaca atctttttga tttctgtcat
    75481 tgtcattatt gtcggtgaat tgatgtccat cggtaaaact ctatctatcc agcaattagg
    75541 agaaacgttt gccactatac ctgtatggaa aagcggacta atgctattga ttggtcttat
    75601 ctctgtgtta cctatggttg gttatgatat tattttgaat cggatgcttg aacaaaagca
    75661 gaatcctcgt tacctgtttg agacaagctg gctgatcaat acgatcaaca atattgccgg
    75721 tttcggcggg tttgttagtg tggggcttcg ctctgaacta tatggacaaa agaaagagag
    75781 cagaaacgtg atccaagcat tatcgaaagt tttcttgttt ttgatggctg gactttctat
    75841 ttacagtctg ctttcttttt tacttgtagt cttcacgcca gttgctcctt ttttgaagca
    75901 atattggata tggttgatcg gcggcggtct atattttcct gtcgtctttt tatttactac
    75961 tttcaaaaag aaaggattcc ttggtggtac ctcacttaaa actcaaggac agctattgct
    76021 agtttcattg ttggaatgga ccggtgtcct gtttagtttc ctttctgttg gttatttaat
    76081 ggaaatccgg attgatctgt ggcaaacaat tccattattc attgctgctt cagtgatagg
    76141 aatcgtgtct atgattccag gagaaattgg gagtttcgat gtaatgatga ttatcgggtt
    76201 gtccgctatt ggtgtgccaa gagaaatagt cgttgtgtgg atcttgctct atcgcctgtt
    76261 ctactacatc attccgtttt tgattggaat cgtctttttc ttcaaaaata tcggttccac
    76321 ttttgatcag cgttattctg gtatccctaa acaattagca acagaaattg cgcataaaat
    76381 cgtggttgtc ttattatact tttctggaat catgctagta ctttctgcca ctatcccgca
    76441 agcttttaca gagtttcgct ggcttcatag tttgaaccca ttgaaatttc attttattat
    76501 ccaatttcct agtatactat tgggatttct tttaatcatt atgggcagag ggatcgcagc
    76561 acgtgtgaaa cgagcatatt tgcctacaat cttcttgatt gttctagctt tactttatgt
    76621 tttattaagt gattttagtt tcacaccggt ggttttcttg tctattctgc ttctgatcat
    76681 tcttgcttct aaaaacgaat tatttcggga acaattgatc tattcatggg agtggcgtac
    76741 gatcgatgga atcattattg gtgcactgtc cttgctttac atcgttattg gggtatacaa
    76801 tctgccggat ttccctcatc ggcgtcacca ttttatttcc tttttcttat ttccttctga
    76861 aaagatttgg ttttcgggat tgttggccat catcgctgtt agtttcatga tcgttctatt
    76921 tgttcatttc cttcaaggag aaaagaaaca gattggcgaa gcctttaatg aagaaaaggc
    76981 cctgaagatc cttacaactt atggaggaaa ctccgatagt caattgatct tcttaaaaga
    77041 taagcggatg tttgcctatg aaaaagacgg tgaaccaact gttcttctcc aattcgcttg
    77101 cttcaataat aaatgtattg taatgggtga tccttctggt aagaaagaag attttccaga
    77161 agcaatcgaa gcttttatcg aagaaacaga tcgactatgc tatttaccag ttttctacga
    77221 aacaagcgaa gagattgtta tgattttgca tgaattcggc tacgatttca ttaagatggg
    77281 ggaagaagct tatgtagacc tgaatagttt tacaacttca gggaaaaaaa tgaagggcac
    77341 tcgtgctgtc ttgaatcgga tcgaacgaga aggatttaca tttgatgtct tacaaccacc
    77401 attttctact gagcaaatga gtactttcaa aaatatctct gataattggt tgggttcacg
    77461 aaaggaaaaa gggttttctt taggcttttt ctctgaagat tatctacaaa gagcgcctat
    77521 tgcagttgtc aaaaataccc aagaagaaat tgtcgctttt gcaacgatca tgcctactta
    77581 cacgaacaat aaagtaggaa cgattgatct gatgcgatat gattctaaaa aagcaccttc
    77641 tggcagtatg gatttcttgt tcttgcattt gtttgcctat atgaaagaag aacatatcca
    77701 atggttcaat ttagggatgg cccctctttc aaatgtcgga acttccagaa agagtttttt
    77761 acaggaaagg attgcatact tggtttacga gtttggttct catttttatt ctttccatgg
    77821 attaagagaa tataaaagca agtacgctac aaactgggtc tcacgttata ctctatattc
    77881 tagagatagt tggattgcat atgttatgat tgctttgctg attattgata atgcgccagt
    77941 tgagcagaca caaggtagaa taagtggttt gaaaaaatgg atacgaagaa agtattaaaa
    78001 actgcatacc gatagatgaa cactctcatg atgaataaaa gagactgaag taaaacgaaa
    78061 gttcatgctt ctacaatctc ttcttataca attgaagaac aaagagcggt ccaaacagaa
    78121 atcctgtttg gaccgctctt ttattttaaa catttttcat taccaaaata ttcgagtcgc
    78181 gtttcttatg cttcgatctc tgtaacgata cctgaaccta ctgtccgtcc gccttcacga
    78241 attgagaatg tcgttccgtt ttcaacagcg attggatgga tcaagtcaac atcaatcgtc
    78301 acgttgtcac caggcatgac catttctgta tcttctggca atgtgattgt tcctgtaaca
    78361 tcagttgtgc ggaagtagaa ctgtggacga tagttgttga agaatggagt atgacgtcca
    78421 ccttcttctt ttgtcaacac gtacacttct gctttgaatt ttgtatgtgg agtaatggaa
    78481 cctggtttag caagtacttg tccacgttcg atatcgtcac gttggatccc ccgtaacaac
    78541 acgcctacgt tatccccagc ttccccgtaa tctaacgttt tacggaacat ttctacacct
    78601 gttacaactg ctttttgtgt ttcaggtttg atccctacga tctctacctc atcaccgaca
    78661 cgaacagcac cacgatcaat acgacctgag gcaacagttc ctcgacctgt aattgagaag
    78721 acgtcttcca ctggcaatag taatggttta tctgtatcac gttctggtgt tgggatatat
    78781 tcatctactg tatccatcaa ttccataata gcagcttcag catctggatc gccttgcaat
    78841 gcttttaatg cggaaccttt gatcacagga gtgtcatcgc ctgggaaacc atattcgctc
    78901 aataactcgc gaacttccat ttctaccaaa tcgatcaatt cttcatcatc gactaaatca
    78961 actttgttca agaaaacaat caggtatttt acaccaactt ggcgtgacaa taaaatgtgt
    79021 tcacgtgttt gaggcatcgg accgtcagtt gcagaaacaa ctaagattgc accatccatt
    79081 tgtgctgccc ctgtaatcat gtttttcaca tagtccgcgt gtcctggtgc atcaatatgc
    79141 gcgtagtggc gtttttctgt ttcatattct acgtgtgcgg tattgatcgt aatcccacgt
    79201 tcacgttctt ctggagctgc atcaatacta gcatagtctt gaggatttgc taggcctttt
    79261 ttgcctaata ctgtagtgat cgcagcagtt aaagtagttt tcccatggtc aacgtgtcca
    79321 atcgtaccaa tgttaacgtg tggtttactt ctgtcataat gttcttttgc cataatttac
    79381 aaacctccta aataatatag atcactgaaa aatttcagct ctcacctttt tattatagtc
    79441 tttttttgac taaatcaaaa agaatacgtc cgacttactt tttgtaagat tcgaactaag
    79501 cttattatac gtcaatattg gtcaaagtca actaagagca ctcgtctgtc ttatacgcga
    79561 aaaaagtacc ttttataaat aaaaaaatgt gttttttgag aaaaacttca agatagtcct
    79621 taactttctt ttgaagcaat aagaacaaaa tgaggaaaag tttaaaattc agctgttgcc
    79681 aacaactttc aatagacgaa atcttctatt tcgttaataa taaggaaaga ggtgaagtac
    79741 atgaaaaaat taattggcgt acttgtcgtt ttaggcatat ttctcggcgg aagttttgca
    79801 gcttatcatt atttttacgg cggcgaagct tattatacaa aaatcacgac gaatggcgaa
    79861 atgtcagcta cccaagcaga taacggagag aaattcacga cttataccta caagcaagcg
    79921 gcttataata aagatggtga aaaaaaacag gtgacgcttc gcgaagaaag agaacatcca
    79981 ttaaagatga atgcgtatct taaactcaaa gtcaatcctc gtaaaggtgt attaagctgg
    80041 gaagaaataa aggcttctga agtgcctaaa aatgcggcag aaaaaatcga taattagtaa
    80101 atggtcttaa aattttatta gataaaaaga ggctgggaca gaagcgttta actccaagaa
    80161 ataagaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt
    80221 gttctttact tttgtctcac gctctttttt tgttatattc gtcttttccg atccttctct
    80281 tgacacaatt catttaactg atcactggta cttgaacacc taatattcta gtcattttta
    80341 taaacgattc aatacttcat ccacattttt gatcatcacg gatatcgtac catccggatt
    80401 attcacaaat tcaatcaaat ctgggttgcg atatacatct actggtacga tcagttcgat
    80461 cccgtttgat agcttgaatt tttgttttcc aaattttttc tcagaaattt cttgtacttc
    80521 ttttacagga ggtgcttctg ggacaaatcc ttgctcttgt acttcttctt taaaagccat
    80581 ttttgccgtg atgttttcct tgaagacttt ttccgcaatc atctcatgat ccaaccgacc
    80641 tttttcttct atcgtatcat agacagcttc tttcaaatca gccatgatat caaattcttc
    80701 tgtctcaaat ttttttccaa gatgcttggc tactttttgg atcactttta cattttcttc
    80761 taaagaaggt gcaggaacac tttcgatcac tcttccagaa aaataaagct cttttttgcc
    80821 agaaaactca tatttttttt caattaattc gtaggtccaa tcagtcaaat tcacagcgaa
    80881 tgcttcatct gctttttgtg ttttcgcccc tagaattgct cgattcaaaa tcaagcggtt
    80941 gtcgatccct gcttcgtctg cttcaacaaa gtgagtgtaa gcctcacgat aattgacttt
    81001 caataaagat aagtacatca ctgtatctaa ttcatacaag acaacaaata catctgcact
    81061 aggtgcttct tcactttctt ggtagatatc gaaccaccgc tgaacgattg cttcacttgc
    81121 ttccacaaaa ttttcttgag cgattttaag tgattcaacg atcatgcttt cttctgctag
    81181 ttgtccagtt tttgtttgag cagaagagat tttttggatc ttcttttgaa gaaaatcacg
    81241 aatatattct tttgtcagat ccagctcttt ttgtgaaaag actggagaac cagtttctct
    81301 gtcaataata tgcagaattg ctttttttaa atagatatcc attttctcat tcccttctgt
    81361 cttagtttac ggaaaaaaaa tcaaaaatac cagaatcaaa atcattttct tttaaacaaa
    81421 aatttcaaga gtagaaacaa aatggattct ttttttcgaa aaatgcgcta cacttatagt
    81481 tgaacgaata aaaaaggagc tgaaagtatg aattcattaa caagcactta tcgtctatca
    81541 aatggttacg aaatcccggt agtaggattc ggcacatggc aaacacctga tggggatgtc
    81601 gctgtttcct ctgttaaaga agcgctggct gcaggttatc gccatatcga tactgcccaa
    81661 ggatacaaga atgaagaaag cgtcggccaa gcgattaagg aaagtggaat tcctagagaa
    81721 gagatattct tgacaacaaa attatggaat gcaaaccaca gctatgaact tgtgatgtct
    81781 tcttttgaag aatcattgaa gaagctccaa acagattatt tagatctgtt tttgatccac
    81841 tggccaaacc ctgttgcttt tcgtgataat tgggaacaag caaatgcaga tacatggcga
    81901 gcatttgaag aactgtatga agcaggtaaa atcaaagcaa tcggtgtgag caatttctta
    81961 ccccaccatc tagatacact agcaaaaaca gcaaaaatta tgccgatggt caatcaagtt
    82021 ttccttgcac caggcgaatt acagacaact gtagttgagt atgcaaaaaa acatgatatg
    82081 atcctcgaag catatagtcc attaggcact ggtaaaatct tcgatgtgcc tgaaatgaaa
    82141 caaatcgctg aagcacatga caagacgatt gcacaagttg ctttgcgttg gtcgctgcaa
    82201 cacgaattcc ttcctcttcc taagtcagtg acaccaagca gaatcaaaga aaatactgaa
    82261 ttatttgatt ttgaattaac agaagaagaa atgaaacaaa tcgatcagtt agatggtgta
    82321 gtcggcaaag caaaagaccc agatactacc caattttaag tcgaaaggtg attttatgga
    82381 tccaatcgaa cgcttgaatt ctctctcgga agaagttacc cagacttttc attccgattt
    82441 tgtctttttg atcgatgcag agaaaatcca acattttcct gcaagaaact ggacacatga
    82501 tcaaatcatc gaagaactga aaaaacggtt cgatcattcg ctaatggtca aaccctggca
    82561 cgaacatgaa gtcatttatt cacctgaact acctgttttt gccttgatac cgaaaaaata
    82621 gaaagaatgg aacggattat gtatatatac ataatccgtt ccatttttta tttttctttc
    82681 aaaaaacggg aaaatacgca aatttttctg ttgtaatttg cgtatacgca agtttttttg
    82741 cattactcct tttcatttgg ctgttttttt cttataatcg aaagggtatg agcgaaggag
    82801 agaacatatg aagaaaaaac agaaacaaaa attattaaat caattccgtc cttcattgga
    82861 tggtgtaaga ggacaattat tccgttctct agaagaagag agcttgcgac gttatggttt
    82921 gccgttacaa gtcatgcttg atcccaaaaa ccgtcaaatc ttaacgatcg gattagctgg
    82981 gaaaacaaat gaagatacac ggatcaacgt gccaatcgat gacaacttca atactgtatt
    83041 aaagagaatt cgttcaggag aaaacgggat tttcgaacga ttccgtgaca atcttcttat
    83101 tgaaatcgtg tcttactgga atgatcaacg attgaaaaac gatgaaccaa ctgcgcaagc
    83161 tgttgcgaca ccagttacag aagaaacagt cagtgtgcca gaagaaaaaa cggaaaaacc
    83221 aaaagcggac acaactgaaa atacacgctc aactgatacg aatgccgctt taagttttgc
    83281 tgatttttca aaagccgttg ctgagtatcc taaattttac acagaacaaa atgaacatga
    83341 agtagttatt aatgaaaaaa atgctgatga acctcgtaaa ttagcaacta tttctatgac
    83401 tactgaaaat gagttcgtta tcgaaaaagc actcgaacgg aaatacaaag tcaaattgac
    83461 tttgattcct ttgatcgaac aatttgcagc gacagcgatt actgatcgat aaaaattcta
    83521 tttgtcataa aaggctggga tttaaaatcc cagccttttt agtcatataa taatttctac
    83581 agcaagtaaa aagaaattcc ttctaacatc aggctcaaat atcagcttga cctgtccaac
    83641 aaatcaccta tgatcctaca atgttaggcg acttctcttc tacctgaaga tatcttggag
    83701 ttgcctgatt tcttaagatg cctaacctta actataaaaa attctaataa taaacatatt
    83761 ttctgataga gaaaaaa
//
LOCUS       DAYYBH010000004        78947 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000004 DAYYBH010000000
VERSION     DAYYBH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.004"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(388..2023)
                     /locus_tag="ACHAUG_000299"
                     /pseudo
     CDS             complement(388..2023)
                     /locus_tag="ACHAUG_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2346..2825)
                     /gene="rlmH"
                     /locus_tag="ACHAUG_000300"
     CDS             complement(2346..2825)
                     /gene="rlmH"
                     /locus_tag="ACHAUG_000300"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8479424.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3253..4542
                     /locus_tag="ACHAUG_000301"
     CDS             3253..4542
                     /locus_tag="ACHAUG_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8479425.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4581..5186)
                     /gene="ytpR"
                     /locus_tag="ACHAUG_000302"
     CDS             complement(4581..5186)
                     /gene="ytpR"
                     /locus_tag="ACHAUG_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8479426.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5391..5855)
                     /locus_tag="ACHAUG_000303"
     CDS             complement(5391..5855)
                     /locus_tag="ACHAUG_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8479427.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5877..6197)
                     /locus_tag="ACHAUG_000304"
     CDS             complement(5877..6197)
                     /locus_tag="ACHAUG_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8479428.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6341..7420)
                     /gene="pepA"
                     /locus_tag="ACHAUG_000305"
     CDS             complement(6341..7420)
                     /gene="pepA"
                     /locus_tag="ACHAUG_000305"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8479429.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7620..7970
                     /locus_tag="ACHAUG_000306"
     CDS             7620..7970
                     /locus_tag="ACHAUG_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8479430.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8193..9140
                     /locus_tag="ACHAUG_000307"
     CDS             8193..9140
                     /locus_tag="ACHAUG_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8479431.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9197..10615)
                     /gene="pepV"
                     /locus_tag="ACHAUG_000308"
     CDS             complement(9197..10615)
                     /gene="pepV"
                     /locus_tag="ACHAUG_000308"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8479432.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10764..11609
                     /locus_tag="ACHAUG_000309"
     CDS             10764..11609
                     /locus_tag="ACHAUG_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8479433.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11613..12053
                     /locus_tag="ACHAUG_000310"
     CDS             11613..12053
                     /locus_tag="ACHAUG_000310"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8479434.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12103..12297
                     /locus_tag="ACHAUG_000311"
     CDS             12103..12297
                     /locus_tag="ACHAUG_000311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479435.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12433..13119
                     /locus_tag="ACHAUG_000312"
     CDS             12433..13119
                     /locus_tag="ACHAUG_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8479436.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13527..14216)
                     /locus_tag="ACHAUG_000313"
     CDS             complement(13527..14216)
                     /locus_tag="ACHAUG_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479437.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14191..16320)
                     /locus_tag="ACHAUG_000314"
     CDS             complement(14191..16320)
                     /locus_tag="ACHAUG_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8479438.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16368..17705)
                     /locus_tag="ACHAUG_000315"
     CDS             complement(16368..17705)
                     /locus_tag="ACHAUG_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8479439.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17957..18316)
                     /gene="rplT"
                     /locus_tag="ACHAUG_000316"
     CDS             complement(17957..18316)
                     /gene="rplT"
                     /locus_tag="ACHAUG_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8479440.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18369..18569)
                     /gene="rpmI"
                     /locus_tag="ACHAUG_000317"
     CDS             complement(18369..18569)
                     /gene="rpmI"
                     /locus_tag="ACHAUG_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8479441.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18641..19165)
                     /gene="infC"
                     /locus_tag="ACHAUG_000318"
     CDS             complement(18641..19165)
                     /gene="infC"
                     /locus_tag="ACHAUG_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8479442.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(19209..19334)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19485..20117
                     /locus_tag="ACHAUG_000319"
     CDS             19485..20117
                     /locus_tag="ACHAUG_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8479443.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20119..21024)
                     /locus_tag="ACHAUG_000320"
     CDS             complement(20119..21024)
                     /locus_tag="ACHAUG_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8479444.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21109..21768)
                     /locus_tag="ACHAUG_000321"
     CDS             complement(21109..21768)
                     /locus_tag="ACHAUG_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8479445.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22084..23028
                     /gene="mvk"
                     /locus_tag="ACHAUG_000322"
     CDS             22084..23028
                     /gene="mvk"
                     /locus_tag="ACHAUG_000322"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8479446.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23021..23998
                     /gene="mvaD"
                     /locus_tag="ACHAUG_000323"
     CDS             23021..23998
                     /gene="mvaD"
                     /locus_tag="ACHAUG_000323"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8479447.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24011..25096
                     /locus_tag="ACHAUG_000324"
     CDS             24011..25096
                     /locus_tag="ACHAUG_000324"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8479448.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25093..26157
                     /gene="fni"
                     /locus_tag="ACHAUG_000325"
     CDS             25093..26157
                     /gene="fni"
                     /locus_tag="ACHAUG_000325"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8479449.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26406..27209)
                     /locus_tag="ACHAUG_000326"
     CDS             complement(26406..27209)
                     /locus_tag="ACHAUG_000326"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8479450.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27229..28071)
                     /gene="kduI"
                     /locus_tag="ACHAUG_000327"
     CDS             complement(27229..28071)
                     /gene="kduI"
                     /locus_tag="ACHAUG_000327"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8479451.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28087..29094)
                     /locus_tag="ACHAUG_000328"
     CDS             complement(28087..29094)
                     /locus_tag="ACHAUG_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8479452.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29101..29745)
                     /locus_tag="ACHAUG_000329"
     CDS             complement(29101..29745)
                     /locus_tag="ACHAUG_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8479453.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29845..30603)
                     /locus_tag="ACHAUG_000330"
     CDS             complement(29845..30603)
                     /locus_tag="ACHAUG_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8479454.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30730..31881)
                     /gene="fucO"
                     /locus_tag="ACHAUG_000331"
     CDS             complement(30730..31881)
                     /gene="fucO"
                     /locus_tag="ACHAUG_000331"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8479455.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31963..32274)
                     /gene="rhaM"
                     /locus_tag="ACHAUG_000332"
     CDS             complement(31963..32274)
                     /gene="rhaM"
                     /locus_tag="ACHAUG_000332"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8479456.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32276..33121)
                     /gene="rhaD"
                     /locus_tag="ACHAUG_000333"
     CDS             complement(32276..33121)
                     /gene="rhaD"
                     /locus_tag="ACHAUG_000333"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8479457.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(33135..34391)
                     /gene="rhaA"
                     /locus_tag="ACHAUG_000334"
     CDS             complement(33135..34391)
                     /gene="rhaA"
                     /locus_tag="ACHAUG_000334"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8479458.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34388..35848)
                     /gene="rhaB"
                     /locus_tag="ACHAUG_000335"
     CDS             complement(34388..35848)
                     /gene="rhaB"
                     /locus_tag="ACHAUG_000335"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8479459.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            36001..36870
                     /locus_tag="ACHAUG_000336"
     CDS             36001..36870
                     /locus_tag="ACHAUG_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8479460.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36996..37895)
                     /locus_tag="ACHAUG_000337"
     CDS             complement(36996..37895)
                     /locus_tag="ACHAUG_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8479461.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38060..38884)
                     /locus_tag="ACHAUG_000338"
     CDS             complement(38060..38884)
                     /locus_tag="ACHAUG_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8479462.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38895..40190)
                     /locus_tag="ACHAUG_000339"
     CDS             complement(38895..40190)
                     /locus_tag="ACHAUG_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8479463.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(40206..40685)
                     /locus_tag="ACHAUG_000340"
     CDS             complement(40206..40685)
                     /locus_tag="ACHAUG_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8479464.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40691..41719)
                     /locus_tag="ACHAUG_000341"
     CDS             complement(40691..41719)
                     /locus_tag="ACHAUG_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8479465.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41736..42842)
                     /locus_tag="ACHAUG_000342"
     CDS             complement(41736..42842)
                     /locus_tag="ACHAUG_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8479466.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43071..45668)
                     /gene="adhE"
                     /locus_tag="ACHAUG_000343"
                     /gene_synonym="adhC"
     CDS             complement(43071..45668)
                     /gene="adhE"
                     /locus_tag="ACHAUG_000343"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8479467.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45973..46353)
                     /gene="yajC"
                     /locus_tag="ACHAUG_000344"
     CDS             complement(45973..46353)
                     /gene="yajC"
                     /locus_tag="ACHAUG_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8479468.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46450..47595)
                     /gene="tgt"
                     /locus_tag="ACHAUG_000345"
     CDS             complement(46450..47595)
                     /gene="tgt"
                     /locus_tag="ACHAUG_000345"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8479469.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47813..48511
                     /locus_tag="ACHAUG_000346"
     CDS             47813..48511
                     /locus_tag="ACHAUG_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8479470.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48679..51318)
                     /locus_tag="ACHAUG_000347"
     CDS             complement(48679..51318)
                     /locus_tag="ACHAUG_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8479471.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51937..52968)
                     /gene="queA"
                     /locus_tag="ACHAUG_000348"
     CDS             complement(51937..52968)
                     /gene="queA"
                     /locus_tag="ACHAUG_000348"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8479472.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            53161..54335
                     /locus_tag="ACHAUG_000349"
                     /pseudo
     CDS             53161..54335
                     /locus_tag="ACHAUG_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            54339..54980
                     /locus_tag="ACHAUG_000350"
     CDS             54339..54980
                     /locus_tag="ACHAUG_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8479473.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54977..55894
                     /locus_tag="ACHAUG_000351"
     CDS             54977..55894
                     /locus_tag="ACHAUG_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8479474.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55891..56562
                     /locus_tag="ACHAUG_000352"
     CDS             55891..56562
                     /locus_tag="ACHAUG_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8479475.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(57103..57591)
                     /locus_tag="ACHAUG_000353"
     CDS             complement(57103..57591)
                     /locus_tag="ACHAUG_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8479476.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57792..58058)
                     /locus_tag="ACHAUG_000354"
     CDS             complement(57792..58058)
                     /locus_tag="ACHAUG_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8479477.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(58058..58321)
                     /locus_tag="ACHAUG_000355"
     CDS             complement(58058..58321)
                     /locus_tag="ACHAUG_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8479478.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58842..58982
                     /locus_tag="ACHAUG_000356"
                     /pseudo
     CDS             58842..58982
                     /locus_tag="ACHAUG_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(59124..60947)
                     /locus_tag="ACHAUG_000357"
     CDS             complement(59124..60947)
                     /locus_tag="ACHAUG_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8479479.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61545..64250)
                     /locus_tag="ACHAUG_000358"
     CDS             complement(61545..64250)
                     /locus_tag="ACHAUG_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8479480.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64556..65503)
                     /locus_tag="ACHAUG_000359"
     CDS             complement(64556..65503)
                     /locus_tag="ACHAUG_000359"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8479481.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65693..65962
                     /gene="rpsN"
                     /locus_tag="ACHAUG_000360"
     CDS             65693..65962
                     /gene="rpsN"
                     /locus_tag="ACHAUG_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8479482.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(66138..66500)
                     /locus_tag="ACHAUG_000361"
     CDS             complement(66138..66500)
                     /locus_tag="ACHAUG_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8479483.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66511..67632)
                     /gene="alr"
                     /locus_tag="ACHAUG_000362"
     CDS             complement(66511..67632)
                     /gene="alr"
                     /locus_tag="ACHAUG_000362"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8479484.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67648..67998)
                     /gene="acpS"
                     /locus_tag="ACHAUG_000363"
     CDS             complement(67648..67998)
                     /gene="acpS"
                     /locus_tag="ACHAUG_000363"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8479485.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(68128..69639)
                     /locus_tag="ACHAUG_000364"
     CDS             complement(68128..69639)
                     /locus_tag="ACHAUG_000364"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8479486.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69961..71313)
                     /gene="murF"
                     /locus_tag="ACHAUG_000365"
     CDS             complement(69961..71313)
                     /gene="murF"
                     /locus_tag="ACHAUG_000365"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8479487.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71466..72542)
                     /locus_tag="ACHAUG_000366"
     CDS             complement(71466..72542)
                     /locus_tag="ACHAUG_000366"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8479488.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72738..74483
                     /locus_tag="ACHAUG_000367"
     CDS             72738..74483
                     /locus_tag="ACHAUG_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8479489.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74763..75200)
                     /locus_tag="ACHAUG_000368"
     CDS             complement(74763..75200)
                     /locus_tag="ACHAUG_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8479490.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(75184..76557)
                     /locus_tag="ACHAUG_000369"
     CDS             complement(75184..76557)
                     /locus_tag="ACHAUG_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479491.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76879..77367)
                     /locus_tag="ACHAUG_000370"
     CDS             complement(76879..77367)
                     /locus_tag="ACHAUG_000370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8479492.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(77389..77991)
                     /locus_tag="ACHAUG_000371"
     CDS             complement(77389..77991)
                     /locus_tag="ACHAUG_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8479493.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(78060..>78947)
                     /locus_tag="ACHAUG_000372"
     CDS             complement(78060..>78947)
                     /locus_tag="ACHAUG_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8479494.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT        22740 a        16762 c        14357 g        25088 t
ORIGIN      
        1 tgcagattag aagcatgtaa ttttttctca gaaacaaacc actcattgga aaagacatga
       61 atcaaatcat cacttacttg tgtaaaatag cgacgtttaa tgacaaagat atcttcattg
      121 ctatctagtg taccttcatt tctttgtgct taaattcaaa gataattttc tcaataaaga
      181 tttttgattc cagtaagatt cctttactcc ttttacaaca tagcgttgtc ctttgttttg
      241 aaacatcttc ttcccctctt tgccattaaa aatagcccaa ttctctataa agaagattaa
      301 gcttcattat tataatataa agtttaaatt tggtttatta cttacttttt cttattctct
      361 agtaagctaa tattgaaaaa aatgtaatca gatcattatt tttatatgtt gtaacttcct
      421 gataagaaaa ctaattaaaa gaaagaggta actacataaa ctaattaaaa aagctaactt
      481 catagttgga ggataaatgg aagaaacttc aatgattgtt tctccttgtg ttccttcaat
      541 cgacactagt cctattatca atctctcttt tttgtccatt tacttttgcg actgttcccc
      601 aataatcaaa tacaggaagc gttatttttt gtcctttaga ttcatgtact gtatagacta
      661 ttttcgaatc catcacttga taaggcatag tatgccattc cttgccaatt cttacttcat
      721 gattctgcaa atgttctttg tattgtaatg cttccgtggg tgcatagtca taaagtcctc
      781 cgggaaggag tgtcgtgtaa cgaataagat tttcttgatt cattaccaag tgatctctct
      841 gagtgacaac tttatgttca tagtttgctt ttgttgcagc agatacagcc aacagtaata
      901 aaacaattgc cgttatatta actacttttc tttgcttagc tgatgtgctc ttcatgaaaa
      961 cgaacgataa aatcaacgag ccaaacagta cttgaaagcc taagattcgc caaggaaatt
     1021 gaatcatttg aatgggtgta ttttgtaata aagaccatgg aaaaaatttg gtggataaga
     1081 gccaagagac taccgcacta aggtaaatca ctttttcttt gcgattaaat cctttaaaaa
     1141 agaagagtgg acagatcaaa gaaataatac caatgattcc taccgtaaag gttgtcaggt
     1201 cattattcat cgcaccaatc aaactatccg agacatttaa tgcttggtgt tgtaaggtcg
     1261 ttttatctgg ctcattaatc gattgaacca tgatttgtcg gaacatcgga taccaaaagt
     1321 atgctgttaa aagaatcgtc attcctactg cctttgacaa ctccacaatc aaccggatgc
     1381 tttctttttt tcttgtttca cttactacaa agccgcctaa gactagtaag ccaacggcta
     1441 ttacactcat aaaagtggaa agaacatgtg tataaatgag taagctcata ctgactgcta
     1501 atggaattgc acatggcttt ttctcataaa tcagtgcgta agcataatac ataactatcg
     1561 gtataaccgt aagtgcaatc gcttcagcaa tagcagaacg atgataaata tcaatcgtac
     1621 ggtacccata aaatgtatat aaacagctaa atataaaagc cgcatcgatt ctttttaaaa
     1681 attttagtcc ataatgaaac atcagcatga tagtacaaac attcaatagc caaacatata
     1741 agatataact aacgattaaa ttattactta tccaataaaa aatgactgcc ggaaaaaagg
     1801 tcaagtaggg ataaaaataa ttcactccgc tcccataatg attaaacgtc gtaaaattga
     1861 ttggaccaga aaaaattgaa gataaccctt tgactctagc aaaatggaag gcaatatccc
     1921 ccgatgtaga aatagtatga acatttctaa aataattgat tgaataaagt ccagcaataa
     1981 ttgctaggag aatggacaat attatttttt tctttacttg cattacttaa ttatatactc
     2041 ctttcatgga ttgctttatc tagtataact taaattttaa ataaatcgtt atttattttt
     2101 actctcactt aaaaaagcac taaaattata agagagggag gtaatggtct gaacaccaag
     2161 aattgttcca acatcgaatt gcttaactaa tgagttcaaa gcttaattat gtggctctta
     2221 tatttaccga gcgtggattg cgatctcttt agcgaaacaa acatctttca taaaaaaaac
     2281 agccatatcc gctttcttgg atatgactat ttactactac tttactatct ttcattcttc
     2341 ttgtctcact tatgataagg cgcccccgca ttgatccgga aggcacggta gatttgctcg
     2401 acaaggatca gacgcatcaa ttgatgtgga tacgtcattt ttccaaaaga gatctgagca
     2461 ttgcttcttt tcatgacggc ttcgctgagt cctaaagagc cgccgataac aaaaacaagg
     2521 tggctttttc cttggatacc taattggtcg atttgtttag caaaagattc acttgtagga
     2581 tttttccctt caattgctaa ggcatagacg tattcttgtt ctttgatttt cgctaagatc
     2641 cgttcccctt ctttttcttt tacttgtctc atttgcgctt cgcttaaatt ctccggggct
     2701 ttttcgtcag gaacttcgat caattcgatt tttgcatagg cattcagccg tttgacatac
     2761 tcattgatac cttggatcag gtatttttct tttaattttc ctactgagat aattttgata
     2821 ttcatgtttt ctctcctttg gtcttcatgc tcactttgtt tattataatc aattttttcg
     2881 tctatatgtt tattttggct atttttgtta aaaagatttc cccaacagtt atgcacaaca
     2941 tccacaggtt tatccacaga aaacagtgtt attttacctt atttcatcac attcattagg
     3001 caaacatacg ttttataaga atttatccac tttatacaca ggctgtggat atgttttatc
     3061 ccttattttt ccaatcctat ccgcgtagtt ttacacagta tctgtggaaa aagaaaatca
     3121 aatattttca tctgaaaaca atgatttttc ctaaagtttt tcataaaaag ttcacagtct
     3181 caaagtagac taaagtcaac aaataagact atactaacag tagataaatg aatgtaattg
     3241 gaggaggaac acatggatag aaaaaatgtg acaccaaaga tgaaaaaaag taaaaataat
     3301 agcctctggc gtaagttagg tctggggctt gttggaggga tcgttggagg actactgaca
     3361 gctggcattt tttatgcagc gatgggcact ggcaacaata gttctactgc tacaagcggc
     3421 aatcagaatt ctgccggtga aacagttgtg gaaaatgtca aagtcaatgt agattctgat
     3481 atcaccaaag cggtagataa agttcaagac gctgtcgttt ctgttatcaa cttacaaagt
     3541 caaaaccaat caagcggttt tggcggacta ttcgggcagc aagaagaaag tagcagctca
     3601 gatgatagta atttggaagc ttatagtgaa ggaagcggcg tgatctacaa aaaagacggg
     3661 aatactgctt atgtagtcac gaataatcac gtagtagatg gccagcaagg tcttgaagtc
     3721 ttgatgaaag acggtacgaa ggtaaaagct gaattagttg gaacagatgc ttattcagat
     3781 ttagcggtgt tgaagatcaa ttcagacaaa gtcgaaacag ttgcctcttt tggtgactca
     3841 agtgctctga aggttggtga gcctgcgatt gcgattggtt ccccactagg atctgaatat
     3901 gcaaattctg tcacttcagg aatcatttct tccttgaacc gacaagtgac aagtacgaat
     3961 gaatcgaacg aaacagtcaa tatcaatgcg atccaaacag atgcagcaat caaccctgga
     4021 aattctggtg gtcctttagt taatatcgaa ggacaagtta tcggtatcaa ctcaagtaag
     4081 atcgcaagta cttctgaatc tacttctaat gtaagcgtag aaggaatggg atttgctatt
     4141 ccaagtaacg atgtagtgaa tatcatcaat caactggaaa aagacggaaa agtgacacgt
     4201 ccagctctag gtatcacaat ggtcgactta tctgccgttt ctactcagca acaagaacaa
     4261 atcttgaaga ttccttcatc tgtaactaac ggtgtcatcg tgacaaatgt ccaaacagct
     4321 acaccggctg aaaaagctgg attgaaacaa tacgacgtca ttactaagat cgatgacaaa
     4381 gaagtttctt ctggtgtaga acttcaatct gctttgtata agaaaaaagt gggagattct
     4441 gtcaaaatca cttactatga aggctcgaaa gaaaaaacaa ctactgttga attgacgatc
     4501 gatcaatcgg cattaaaaca aaatcaaaat agtgaaaatt aataaaaaag aggttgtgac
     4561 aaagacttgt cacaacctct ttattttgga aatgcttctc cgactacggc atcctcaggc
     4621 aatactaaga ttcctttttt tgctggagca tttggcaaat gcaattcacg agcggaacag
     4681 atcattccat agctatctac ccctcgcaaa gttcctggcc agatcatcat tccatctggc
     4741 atcatagctc ctggttttgc aacgacaact ttttgtccag cttcgatatt gggtgcaccg
     4801 caaacgattt gcaatactgt accattgtct acttctgtct cagtaatcga taaatggtca
     4861 gaatcaggat gtgggacgca tgtttttacg tacccgacaa cgatcttcgg ttcatgatca
     4921 gcttcaagct gttcttcgaa tcctgcttct ttgatcaatc gattcaattt cgctaattcc
     4981 tcatctgtta agcttacttg gccgttccct tcgattgtta gatgatcgga gatatggaaa
     5041 aagttccaag caacaacttg accttgttca tctttgatcc gagcaacatt ccatttgcgt
     5101 tcaacagtgt tttctgttcc tttatcgtcg gctataataa ccatcaaggt gtctccgacg
     5161 tgtgctttgt tataagcaaa aatcatgttc tttcctccat tatcctttgc tgagatttat
     5221 cttaacaaaa aattgcattc actgcatgaa atttttttca tgctttattt ccgaagaagc
     5281 ctcttctgga acaccattta ttcgtaacaa gggactgtga caagactttt gtcacagtcc
     5341 ctttactgtt ttagttaatt gattaaaaag gtcgtacaag ttccacttgc ttaattactt
     5401 ggctgttctt cttcgtcaga aacgatcaag acatcacaag gtgaatgacg tatcacgtag
     5461 ctggctacac ttcctgtcat aaagcgttcg actgcattta gacctgactg cccaaccatg
     5521 attagatcga tctcatattt ttttggcaat ttgactgcca tggattcacg aacagaacca
     5581 tatgcaataa ttccttctat atttgtgtaa tcaacagatt tcccatattc tttacattct
     5641 tcgatcattt ccttggctgt ttcagtttgc tgatcgacta gattttgatc aataggtgca
     5701 tagcccataa agttataaaa ttgctgatcg attacgtttg ctacatatac tttcccatta
     5761 ttacggcgag ccacttctac cgcttttttg aaagcagcaa gtgcttgttc actaccgtcg
     5821 atacctacta aaatattctt atattcttga ttcatcttga cccctcctaa cagatgtcag
     5881 ccttttgtta cactttctag gaacgattcg atttcctgtt ttgttttacg atctttattc
     5941 actagacgac ctaattcttt tccgtcttgg atcacgacaa aacttgggat accaaaaatg
     6001 ttccattcag cggctacatc tatgtacttg tcacggtcta cttcaataaa ttgccaatca
     6061 gaaaagtcat tttcaatttc aggcatttgc ggtttgataa aacggcagtc tccgcaccag
     6121 ccggcagtaa agaaaaatac actctttcca gtttctacgt agcctgctaa ttcttctaaa
     6181 ctatttggga taatcattct ttgtcctcta ctttctgttt atgtattttt caattaaatt
     6241 atacaccttt ctttcgaact aacaaacatt gatcctcttt ttataaaaaa aatctggcaa
     6301 aaagcaatct tttgccagat tttatagttt cactatcgca ttattttccg tatacgatcg
     6361 tatttactgt tgatttatcc aaacctttca gtgtttgaat caacatttct cgtgcagctt
     6421 caaaatcgcg aatactgaac atcgtttgat gcgtatggat ataacgtccc actacgccaa
     6481 tcactgtact tgggattcct tcgttttgtg tatgagcagc acctgcatct gtaccgcctt
     6541 ttgaaacaaa gtattgataa gggatattgt ttgtttcagc gatatccagc aagtactcgc
     6601 gcaaacgagg aagcatgatc aaacctggat cttggatacg catcaatgta ccttctccta
     6661 aatgtccgaa tgtgccattt ttcgtcactg tatcatctgc tgcagagcag tctactgcaa
     6721 agaacagatc tggcttgaat ttattgacag aagctttaga accacgcaag cctacttctt
     6781 cctggacatt cgcgcctgca atcaatgtat gacctaatgt ttcattttgc aacgcttcta
     6841 aagcttctaa taccattgta cagccatagc ggttgtccca tgatttactg ataatattct
     6901 ttccgttggc tgttttgatc gtttcagcgt aaggaacgat tgtgtctcct ggaaggacac
     6961 cgtaactcat tgcctcttct cttgattcaa acccagcatc gaacaaaata tcggttactt
     7021 ctactgattt ttgtccgctt gttcctcgca ataaatgtgg gggaacggaa gaagaaatac
     7081 atggataatt cttaccagta gaggttttaa gtgtaaaacg ctgagcagaa acaacgtaag
     7141 gattccagcc gcctagaggt acgactttaa aaagcccgtt ttcttggatt tgtgtcagca
     7201 tgaatccgac ttcatccata tgtgctgcga ccattacacg gggtgcatcc gcttcttcat
     7261 gatggcgcag accaaaaatg ccgcccaaac catctaactg taactcatct accagcggtt
     7321 ccatatgact ttccatataa gcgcgaacat cttgttcaaa accgcttgtt ccttgtaatt
     7381 ctgttaactc tttgattcgt tgaaatgttt tttcttccat atgctattcg tctaaatttc
     7441 agactaaact cctctcagac ttatattgat ctgcggataa aatatgattt tttctgcaga
     7501 ccgatcgtat ttattatagc ttattttttc tggtagcggt tatttatttc aaagaaaata
     7561 tgagagtgtg gtaaaataag cacaaggagt aacaagtgag aagaaggagg tatgtccaca
     7621 tgtatgaaga aagcgaacta agctatttta aaggcgggtt agcagttgga atcggtctag
     7681 gattattgag cggggtagct tcaacgattt ggtatcatcg caaaaaagta ttgtcagcag
     7741 accaagtact tgaaaatatc aagtcatctt ttctaaaaga aggtgcaatc gaaggttctt
     7801 ggatctcttt tgaaaaggaa cctattagga aattcgccgt tcggtcgagt gcttatcggg
     7861 gaggtctttc tcgaatagaa gatggcgaaa ctgtttatta tgaattttta gcagatgctc
     7921 atacaggaac cgtattagaa atttcaagaa aaaaaggtgc aacagattaa tacgtcaaaa
     7981 aagtgagaga acgaatacgc acccactatt caaaagagag gtatcatgtc cttcttttgt
     8041 agtgggtggt tttttagata gagctttcca aacatatatt ccagtatttt ctttttaaat
     8101 aaatgatatt tagcgggaaa aactagcatt tcctttgatt atgaggtatt tttaatttag
     8161 cttttgtttt ctatagttaa ggagagtgaa aaatggaaca acaagaaaaa attgctgttc
     8221 tgattccttg ttacaatgag gaagcaacaa tttcaacagt cattgcagac tttaaacgag
     8281 aattaccaga agcagatatt tatgtctatg ataataattc gaccgatcga acatacgaat
     8341 tggccgttca aggtggagca atcgtaaaaa aagaacctcg tcaaggaaaa ggaaatgtga
     8401 tccgccaaat gttctttgat atcgatgccg actattattt aatggtagat ggcgatgata
     8461 cttatcctgc agaagctgta catggtttgc tcgagaaatt acgttctggt gaagcagata
     8521 tggtcattgg tgatcgccta tcaaatggaa cctactttga tgagaataag agacctttcc
     8581 acgattttgg aaataacctt gttagaaata caattactag aatgtataaa acaaaaattc
     8641 ttgatgtcat gaccggctat cgaggattca atcgtatctt tgtcaaaagt tttcctatta
     8701 tgagttctgg ttttcaaatc gaaacggaat tgacgatcca tgctttagat aaaaaattca
     8761 agttcgttga aatcccaatc gattatcgtg atcgtcctga aggcagcgaa tcaaaattga
     8821 atacattctc tgacggattc aaagtgatca tgatgattat taaaatgttt aaagattata
     8881 agcctttgct tttctttggg atttggacca tcttcttctt tttatttgga ttggtcacgg
     8941 ggattcctgt gattcgcgaa tatatgctga caagctttat tacacgcatc ccttctgcga
     9001 ttttaagtac tggattgatg actttagcac ttctttcatt agtaacaggg ttgattttgg
     9061 atacagtcgt cacgaacgct aagaaagaat atgaattgaa tctatatcat gtttatcatg
     9121 aataccacag tagaaagtaa aaaaaggatc aagactgcaa aaataatttg caattcttga
     9181 tcctcttttt aattaattat ttcgccaaac gatagatggc atctgcatag attgctgttg
     9241 ctctgaacaa gtcatcaaga ctcataaatt cgtttgcttg atgcattgta tcagtatatc
     9301 ctgggaacat cgctccataa gcaacgccac gtttcaacaa acgtccgtaa gttccgccac
     9361 cgatgatttg ttcgtagcct ttttctccag tgtggtcttc atagacttgc aataatgtag
     9421 ccaccaatgg atcatccatt ggtacgtaat gtggttccat tacgcgagca cctcgagtaa
     9481 ctgttgcgcc ttcttgcccc actgtttgtt cgatgtcact ttgcaatccg tctactgtta
     9541 caccttttgg aaaacggaaa ttcatattga tgaagttcgc ttcctctgta ccattttctt
     9601 caaaagcaaa tagtccagca ttcattgtca atttgcccat tttttcatct tcataagcga
     9661 ctcctagttt ttctccataa aaatcttcat ggacataagc agctgctaca tggataaatt
     9721 gtttagcaga tcctaggaat tcatagttat ctaagaacaa tgccaagaaa gaacctgcat
     9781 tgataccaga ttgtgggctt gcaccatgtg cgcctttacc aactaattcg atttttacat
     9841 acgtattgtc cgcttcaatc gttccgctta ctggatttgc ttcgatgaat tggtaaaatg
     9901 cttcttccat cgcgatagct gcatctgcac ttggtacttg aagtgctgca gttgctgttc
     9961 ctggcaccat attttcgcga agtccggaaa caaaagattt caagacataa tctcctgcat
    10021 tgtcgccttt gaagcgtaaa gctaaagaaa catttccttt ttcaccattg atgattggga
    10081 attcagcatc tggtgagaaa ccaaaatctg gttcttcttc atgttcgaaa taataagcca
    10141 tatcgcccca gccgctttct tcatcgctgc cgactacgaa gcgaactttt tttgatagtt
    10201 tcaagtcaag ttctttaatg atcttcatcg catagtaagc tgccatactt ggacctttgt
    10261 catcgcttga tccgcgtgca aagatctttc catccttgat tactggttca taaggatctg
    10321 tgtcccagcc atctcctgca ggaacgacat ccatgtgtcc aaatatccct agcgtttcgt
    10381 ctccttcgcc aagatcaata tgccctgcat agttatcgac attttttact ttaaatccat
    10441 cacgttcgcc ataagctaac atatgtttca atgcgtcgcg aggtccaggt ccaaatggtg
    10501 catctggtgt tacttttgag tcatcgcgct cgctgttgac acgtaaaaga tttttcaagt
    10561 cttcaagcaa gtcttcttta cgtgcttcta cttctttttg ccaatcaatt gtcataaagt
    10621 taatcctcca cttatttaag tcttcgtgtt catgatacca caaattacag aaaaaaatag
    10681 ttcaaaaaat ctgacaattc cttttcttat ctgtttttct ctaaaaaaaa tatacgctat
    10741 aatgagtaaa aggaggtttt tagatgctga taaacgaaca actagtgaaa agagtcatca
    10801 cacctcgtcc aaaaatttca cataaaggaa catttggacg tatcgttttg attggcggca
    10861 atgaacaata tggcggagca atcattatga gtgctttagc tgccgtacac agcggagcgg
    10921 gtctcacgac tgtagtaact gatgaaaaaa atcggagtgc tcttcacagt catctgccag
    10981 aagcaatgat tgtagtttgg acggatactg accagtttct ttccgtaata aagcaagcag
    11041 atgtactctt gattggtccc ggattgggta cttcttctaa gagtttggaa cgtttcaaac
    11101 aagtactaga aaaccaatca aaagaacaat ggctggtgat tgatggctct gctttaagtt
    11161 tacttgctgc taatcatctt ttgctaccct atcccgaaca aactgtactt acacctcatc
    11221 aaatggagtg gcaacggttg agttctctac cgatacaaga acaacgggat aaagaaaaca
    11281 aaaagaagca acaggaaatc ggtgctgtgc tcgttttgaa aagtcatgag acaaaaattt
    11341 atggtcacca gcacttctac accaatcctt taggttctgc tgctatggca acgggcggca
    11401 tgggtgacac tttagctggg atgatcacag gatttttagc ccagtttcct caaaatgacc
    11461 aaacgattgc agcagctatt tatcttcata gtttcattgg agaccagctg gcaaaagaac
    11521 aatatattgt tttgccgaca caaatcagcg aagcacttcc gaaatggatg aattattttt
    11581 gtaaaaaatc aatggaaaac gaggcatgag tcatggagaa atttacaggt tatgtgcgag
    11641 aaccttttta ttatgaaaca gatcaaatgg ggattattca tcacagcaat tatatccgct
    11701 ggtttgaaga ggctcgggta gccttattgg tacatctagg gtttccctat aaaaaaatcg
    11761 aagaatctgg gatcatcatc cccgttctgg aagtaaattg cagctataaa gaaatgatcc
    11821 attacggaga aaaagtgagc atccatccaa ctatccaaaa gtatacgggg acacggttgg
    11881 attttagcta cgaaattgtg ggaactgaag acaaaaaatt aaaaactact ggcagtagta
    11941 aacactgttt tttactttct gattcgcaac gattagtgaa gctgagcaaa gtcaatccgg
    12001 agttagatca gttattccaa gcgtacgctt ctttggatga tgtaaattct taataaagac
    12061 cgtctaagca tttttcttgt attgtgtcga gtagttcgta taatgaaaag aaaaaagcag
    12121 gtgatcccca tgtatgtcgt taaagtaatg cacggttaca tcgataaaac tggctgtcgc
    12181 actcgggaaa aaaacttaga caatctgctc attttcaaag acaaaaaaga atccgaagct
    12241 tttgctaaaa gaatcggcgg tcgtgtcaaa ccgatccaag aggtacgacc agattgattc
    12301 aatatttttc ttttcgaggc tctaataggc atgcctatca gggctttttt tgcagactgt
    12361 gtttttatgc ccacacgatt tgtaagcgat ttatgttata atagattata tctgcataga
    12421 aaggaaggtt aaatggttat caataatttg caacagcaag cttatgaatc tatacgtaaa
    12481 caaatcattt attccgagtt agcacctgga agtaaaatct ccgataaagt gttagaagaa
    12541 caactagcta tcgggcgtac accgattcaa gaagcactga tccaactgcg caatcaagag
    12601 cttgtaaaaa ccattccaca gtccggcact tatgtttcaa aaatcgatat ccgatctgct
    12661 tcattagccc gttatacgcg ggaaaagttg gaaaaaccga tcctgcaaga atgcagtgcg
    12721 aaaatgacca agcaagatca agctaaacta gaaaaaatac tagaacaaac cgatcaggca
    12781 gtaatcgctc aaaataaaaa agcttttttt gatttggata ctgattttca ccgaacttgt
    12841 tatgaaattg ctggaaaacg agaaatttgg gattggctag aaagttatag tacgcacttg
    12901 aaccgtttca gatggttacg tttgacgatt tcagaattag actggggtcg agtattagat
    12961 gaacatcaga caatgcttca atcaatgatt gatcataact ttgatgaagt cggatttctt
    13021 tgcacgatgc atttacatat gattatcgag gagcaagaat acgtgattca taattacccc
    13081 gattattttg aagatattca ggacagaccc atcaaataag aggttttgga atcagcgttt
    13141 tgacctgagt attagagaaa gaaacggcaa aatagttcct catattttgc cgtttttttt
    13201 gagctaatac cgagggtcag ctgattgcac accgtttatt gagaggttgt gaaaaagctg
    13261 ccctgcatca agtaataaga acagccaagt aaaaatagct tcttatgttt ttaacttggc
    13321 tggacttatt accgcagatg caagcttttg aacaccgttt tccaagaggt cgtgagcctg
    13381 ccgttcatct ccgagtcaca aggaacaatt tcaaaaaaca gcttctcatg tttgttgaat
    13441 attttgactt gtgaccgagg agttgacagg agaacatcgt ttattcggaa aaaaaggact
    13501 gtgacacgac ttttgtcaca gtctctttat ttgtaatcta aatcatactc cgtacatctt
    13561 ttttctattt tttttatttc ttctaagatc ccttggctac gaatttcatt atatggccaa
    13621 agtaagactg cctctaatac ataaggcgaa aaatcgcttt gattcatctc ttttgaaaaa
    13681 ctcatcgctg ctatctcgct caatgcacga atgtttgatt gatgtgtata aaaaggcaac
    13741 cgccacagca caattttttc gctttgcgta taaattccct taacagaagc ttcgatgtgg
    13801 tgaaatagtt catgtccaat aatcaaagct tgcgctgttt ctttagagat cgaagctagt
    13861 cctgaacatt ttaatttttc taacggctcc gtcatcaatc gaatcccttc atcttctgca
    13921 taagttgcaa aagtgattcg ttcgtttatt tgctctgatt cacaggaaac gattggaaca
    13981 gccatttgtt cacaaatttc tcctacatcg gcaaaagggt actttttttt gatgctacat
    14041 gccgtttcga taccacaatc gattgattgt tgtataagaa ttttctgctt ggacaaagga
    14101 atctttttcc aaaataaatc tcgccgaaac tggtagaatc cccaaatttc ttcggggatc
    14161 tgttgtaatt cgtttagtaa ctcttctaac ttatgtttgt ttgatatttc cggcaacgac
    14221 gatcacttct tcctccggtt ccatagtaga tacttccaga tccatcaaaa gctgtagttg
    14281 ttccagatcc gtcgctgtaa aatcagagac tcttgctcct aagcagagat aaaattctgg
    14341 tcgggcaatc aattcagttt gataaactgc catatcttcc caaagttgtt cgacagctgt
    14401 catataattg gctgcagttg ttttcataca tgaagcatgt ccacgttgga ccttgataaa
    14461 tcctttttga tcgatgatcc gaagacttcc agcatttttt gaacgattct gttcgccgca
    14521 tacataaaag aaaggtgtat tttccaataa gcaaacttca tttttattta atcgcatatc
    14581 ctctgcagca agctctagtg cttcttctgt tgtgatctcc ttggttagat cggttgcttt
    14641 cacttccgta gaaccagtag cgatagccgt tacttttgaa gtctgtggat cgatttctac
    14701 gtggacttcg atcgattctg gtgtagcgcc tgattcaatc gctttattca tcgcttcatt
    14761 tttcaacgat cgtatatctt ctttacttgg cgaagggatg atacgttcta caacatctcg
    14821 gaccattgca agtgctacac caatagaaga aatcacttcg gcgttttcag gaatcgaata
    14881 tttcacaccc attttattac taaagtaagt aatcaatgag gcagcgccgc cccctacacc
    14941 tacaagagaa atctgatctt tctccaagtg atacttgtct gccaattcta aaatcaccgg
    15001 ttcaatctta gcataggatt tttccatgat ctgttctgcg atgtcttcaa ccgtcgtatg
    15061 acaataatca gctaatgctt ggatggcttt tcgtgcagat gggacatttc cataagaaaa
    15121 atgttcttct tgcactaatc caaggacatt ggctgcacat gtatttgtaa tagtcacttc
    15181 ttctccgtct tccatcacta ccttgacgta atcagcaggg tctccttctt taggagagaa
    15241 aaattcaacc ttaggacctt tgattttttc tgtctccgta aatactgcat aatctagacc
    15301 ggcaatatgt gcagaacgtg gaccgacatc gatgattcct gactgattag cccgtaccat
    15361 cgaaccgcct gccacaccta gaacccgaac atctaatgaa gagatatatg ttgggtggcc
    15421 acctacaatt gaataatcga ttgctggacg tccattttta atgaccccga tattggttgt
    15481 tgttccgcct acttcaaaat aaacaccatt agacgctctc aagtacatca aagagcccat
    15541 gactgatgct gctggaccgg aaagcatggt taaaacaggt cgttttttca tttcattgat
    15601 ttccattact ccgccgtctc cgcgcataat catcaaagaa acattgaccc ctgcttctcg
    15661 tacggaatct tctgtagagg ttgccgtatc tagcattttt ggtaaaatag atgcattgat
    15721 tgctgctgtc cgagttcttc ttgtcaatcc atataatttt gtgatgtcag aagccatcgt
    15781 tgtagggatc gattttaccg atgctgcttc ataaacaact cgttctggtt ccccattatc
    15841 tactccaaat gccatagaag aaaccaagac ctccgctctt tctctttcta aagaagaaat
    15901 cgtttctgaa acacggtcta catttaagtg tttgacaggt aagaatgcat tgacaatctc
    15961 tatttttttc ttattcccta aatcgatatc gttcaatcta gtttgtcgtt ttgctaaaaa
    16021 accttctaat ccaccttttg ccatcccgat tacgccgact tttgctacgt ctccttcaat
    16081 cagtgcatta gtcgcttgtg tggtagaatg tgcaacaaac acaacatctt ctggactgat
    16141 attattttcc agcaaacaat tttgaaaaga ctgaacgaca cctgcggcta ctccacggac
    16201 atcatcgtgg gttgttttca ctgaagattt tccgatgatt tcgtgcgtcg cattatcgat
    16261 tgcgactgct ttcgtatgtg taccgccaac atcaattccc attcgcacaa aacgtttcat
    16321 tttcttttcc tccattcatc taatcgtttt tatcgacgtt ttgtttacta ggcgttcccg
    16381 tacaataaga acgtgatcag gcataagatc gctccgatga tccaaccagt tgggatggat
    16441 ttcttcatat attctcgaga agttactttt gtataaccta atccccaagc aacccaagat
    16501 tgtgtaatat ctaaatgctg aggagcaatc gttgtgattg caaataacgg gaacaaaaag
    16561 gctggcgaca tcgttggatt aacagctaat acgacagcta aaatagctga gcctgaaccg
    16621 actagattca ttggaccgcg gaaaaatcct agtggtgtca atatagcaaa tacgattgcc
    16681 aagactaatg gtgattgcgg tatccaatct ccaaaaatag ctgaaaaata aggagaagca
    16741 tacgcagctg aattattgaa catcgctaag gtcatcaaga aaccaaccat tggcgcaaca
    16801 tctactgcgc catctgtaaa taatttcgca aactgacgac agatttcttt ataacctccg
    16861 cgcaattttc cagtaaacag caatgcccag atacctgcta gaataaaacc taagatgatt
    16921 ggaagatcta aaagaacgac ccctaaaact ggtaataata cggctaacca tgagatcatt
    16981 ggtgcgtcgc caccttcagc tggtacgttt gctgccattg catagcgttt ctttttgttc
    17041 attgatatat tagcgaccgt tagtaccaca actaagctta caatcatccc aatcatacca
    17101 atttggaaat agtcattata agtatagctt tcagccgctg gattgaaacc agcataaatc
    17161 gtttgatatt gtttgaaatt gacgatatta gcaaagattc cagccataat cgagcccata
    17221 aatgagaaca gtgcaaccgg tactgaaata cctaatgaca tcaagattgg caacacaata
    17281 actgcaatag aaatcactgg tccgattcct gtcatcgaca tgaatataac agcggttaca
    17341 atacatagta agctcatcgt gattcttggc ttgtcccctc caagttcaac tacctttcga
    17401 atcagggtag ctgcgattcc gctatctact agaacacgcc cgaaaaaggc accaaagaag
    17461 acatttacca agatcgattt ggcgtactca gctggccccg tcgtatagat atgcgtcaac
    17521 acatcaatta ctccttgccc ttccatcgcc gggtttggtg aaaacgtgtt tccgactagc
    17581 gctaatccca tccatacagt agccatgatg gcaaagccga ccatcaagtt gtagcctttt
    17641 acacagtacc agaccatgaa aaagaatgag ataactaaca aaataccaat aataatatca
    17701 ctcatgttat tcctccgtcc attttgtttt tctgataagc aaactaatat atcagtcaaa
    17761 ctcattctaa cttatgtagt tagcgctttc attaaagggt gaccataaat gttcagtttt
    17821 caattaaaaa accttaatta tgctatttta gacttatacg ttattgtcct gtttcatttg
    17881 atggaggatt gtaaataaaa aaagaggaaa gaacataact tttcgttatc ttctttcctc
    17941 tttataattg tttagcttat ttagccaaag cttcttttgc ttggtcagcc aatgcagtaa
    18001 atgctgctgc atcgttaaca gccaagtcag ctaacatttt acggttgatg tcgatttcag
    18061 caactttcaa accgtgcatt aattttgagt agctcaagcc attcatacga gctgctgcgt
    18121 tgatacgagc aatccacaat ttacggaagt cgcgtttctt ttgacgacga tcgcgatatg
    18181 cgtagttgta tgaattcatt acttgttctt ttgctgtttt aaataaagtg tgttttgaac
    18241 cgtagtaacc ttttgctaat tttagcactc ttttacgacg tttacgagtt actgttccac
    18301 ctttaacacg tgccatgtat aattcctcct aataaaaata aatcttcaga ttctgttatt
    18361 tgtacttctt atttcatttt tgctagttgt tggcgaatac gtttgaagtc gccactagaa
    18421 accatgcttg ctttacgcaa ttgacggcgt tgtttttttg ttttgccgtg gaaacggtga
    18481 cttgtaaacg cgcggaagcg tttcaatccg cctttaccag tacgtttgaa acgttttgct
    18541 gatccgcggt gtgttttttg ttttggcatg actatatttc ctcctcaaaa tctttcgtta
    18601 tccgactatc ggctgtccaa cagccagtta gtcattcaaa ttacttactg tcgtttttcg
    18661 gtgccagcgt caagaacatg ctgcgtccgt ccattttcgc tttttgttcc actgttgcaa
    18721 tatccgcagt ttcttcagct aagcgattca agactttttg accaatttct ttatgggtaa
    18781 tggcacggcc tttgaaacgg atagaagctt tcactttatc gcccttttcc aagaacttac
    18841 gtgcattacg aagttttgta ttgaagtcat tcaagtcgat cgtaggactt aagcgaactt
    18901 cttttacatt gatcactttt tgttttttac gcgcttcgcg gtctttcttt tgttgttcga
    18961 aacggtattt accgtagtcc atgattcgcg caactggtgg tttcgcacca ggggcaacta
    19021 acacaagatc caagtttgct tgttctgcaa tattcaatgc ttctgctttt gtcttaactc
    19081 ctaattgctc accatctgaa ccgatcaatc gtaactctcg tgcacgaatg ccgtcgttaa
    19141 ccatcatatc ctttgctatg gtcattcacc tccaaatatt atgagagaaa gaaaagctta
    19201 ccgaataaac ggcaataaaa aaacgagttc tcttacagaa cccgcgcgta cttcacctaa
    19261 tagaaccagt gaaaactatc tagcccagtg acattatcaa ctaaggcgag aagcgggtgc
    19321 ttctgctttt atttctcaac catcatagta tagcaaaaac taatcttttt gtcaatgttt
    19381 ttcatacagt ttgtcttcct tttctgaaaa tgattagatt agcagacttt cttgcagtga
    19441 ttcccgcttc tttgctatac taaagaaaaa cgaggtgaac ccacatgaaa aaaatcgaac
    19501 ctattccacc ttctcttcct cttcttacaa agggagagtt ttatttagaa gatgaggaga
    19561 tcttctctgg tcttctgatc aacgggggtg accaaagcta tctcgatgcc aaaaatatgg
    19621 tattaagaga gagtcatatc caaaaactta cgatgcaaaa aacagtttta cgtcattttg
    19681 aatgcagtaa tgtcattttt gaaaaatgtg atttttccaa tctggagtgg attggtggca
    19741 gtttccacca agtcgtgttt catcaatgta aattaacagg tacgaatttt gccgaaagtt
    19801 atcttcgaga ctgtactttc actgattgta ttgcttcgat ggcttccttt agtagtacaa
    19861 acttgaaagc tgtctccttc gatcattgtc aattagaaga cagcgaattt tatgaggtca
    19921 cttggaaaaa cctttttttg tctgacaatc agttgaccgg ttcgaactgg tttcgaactt
    19981 ctctgaaggg attagacttt acgactaatt tcttcacagc aatcgcttta tctcaagatt
    20041 ctttggcggg actaatcgtc aaccaagagc aagcacttgt gatcgcccaa gcccttggtt
    20101 tgcagatcaa agactagatc aacgaatccg ccgccacagt gcaataagca tccaaataga
    20161 taagaccaaa ctgatgatca aggcgattcc ccatccgaaa acggaatggg taaagggtaa
    20221 ctggagattc attccgaaga atcctgtcac aatagttgga acggtcaaga tcagcgacca
    20281 gaccgtcaaa aatttcatcg tgtcattcaa gttgttattc agcaaattat tataagtgcc
    20341 agagagctgg tctaaaatct gcgaagctaa gttcgtcatt tcaactgctt gtcgtgcttc
    20401 gatcagcgca tcgtccaact cttctttttc cttttctgac aacttgcgat aaatagccaa
    20461 tgccttgatt tgttctaaaa gtactacatt ttgttttgtt cctgtaacta agtaaaccaa
    20521 accgatctct aaatctgaaa gttgcagcag attcttgttc gttgtttttt ctcttaattt
    20581 cagattaagc cgctgacgtt cactattcac ttcctcgatt aaaggaaaaa aggaatcaga
    20641 aatcaaaaac aatgtacgaa acaataaact gtatacactc atgtctggca ttcgttccaa
    20701 aagcgatcga attagtcgaa tcacgtactc ggtattcggc gtggtaaatg taaacaaacc
    20761 gtcttctttt aaaataaaag cgataggact agtttcataa tgattatcga ctttttgctg
    20821 gtgcggaaca tttgatacaa gcagtaacgc ttcatcaaaa gaatcgtact ctacgcgcgc
    20881 ccgttcattt ttatctagag aataagtcag catctcgtca ctaatgccat attcttcttt
    20941 taagttttta acagctagca catcgttttg ctgtacattg atccaaaaat tacggtcttg
    21001 attaaaatag tgtttttccc gcatactcta tcctcctgac atttgtttcc agtataaaga
    21061 aaaaagtcgc tcaaataaag gtttaccctt tatttgagca actgtaaatc aaatggctac
    21121 tttggtttgg cgaatctctg ctaatttttc ttcgatcatt ttcaagactt cttcttttgc
    21181 ttgaggatct tccacaaaat tatagcggtc tccatcgatt tggatttttg gactttcttt
    21241 gtattcttca taccattgat cgtaacgtct gttcaattct ttgtaataat cgtatagact
    21301 tggatcaaag gacaattgtt catacgaacg accacgtttt tcgatacgct ccaacatcgt
    21361 atcaaaagat acacgaatat gaactaataa atccggatgt tttttatggg cagcataggg
    21421 aagttcttcc atcatgtttt ccagcagttc atcatacaca cgaacttctg tttctgttgc
    21481 tcttcctaga tctgcattta gatggaaaag cagtgaatct tcataaatcg aacggtccaa
    21541 tacattattg tcatctttca ttgcttgttt gatgcttgca aaacgtttat tcaaaaaata
    21601 aatttgcagc aaaaaagcat actgttcagg gtttgcataa aataagggca agacttcatt
    21661 atcatcaatc gattcataaa atgcctgact atcaaagtgt tcagccatca tttctgttaa
    21721 acttgatttt ccggcaccaa ttgtacctgc taaaacgatc acgctcattt acctccagct
    21781 cacttcatca ctaaatattg tgtttaactc caaccttttt aaatataccc ccaatatcta
    21841 gtagtgtcaa ttgtattttt ttctagtcaa agaagaaaaa aagtcaaaaa attcttaaga
    21901 aaaaagtgtt ttttctttag aaaacgtatt cttagtttta cttctgagat ggttttagta
    21961 aaataaagga ggaatgtttt ttgagaccct tagtggacaa aaaaattcgt gaagcagcgc
    22021 aactcgtttt gggcgcttta ttttttgctt aaaaatcact tttacgtgag aaggagttag
    22081 attatggcaa actatggcca aggagaatca agcggaaaga tcatattgat gggcgagcac
    22141 gcggttgttt atggagaacc agcgattgcc tttcctttct atgcaacaaa agtcaccgca
    22201 ttccttgaag agctggatgc aatggacgat caactggttt cttcctacta ttcaggaaat
    22261 ttagccgaag ctcctcatgc attaaaaaat atcaaaaaat tattcattca cttaaaaaaa
    22321 cagcatgaca tccaaaaaaa cttgcaactg accattgaaa gcacgattcc tgctgaacgt
    22381 ggaatgggat caagcgctgc agtcgccaca gcagtcactc gtgcttttta tgattactta
    22441 gcatttcctt tgtctcgtga aatactatta gaaaatgtcc agctttcgga aaaaatcgcc
    22501 catggtaatc ctagtggaat cgatgcagcc gctactagca gcttgcagcc gatttatttt
    22561 acaaaagggc atcctttcga ctacttttct ttgaacatcg atgctttttt gattgtcgct
    22621 gatacaggaa tcaaaggaca aacaagagaa gccgtcaaag atgtcgctca cctctttgaa
    22681 acccagcctc atgaaactgg acaaatgatc caaaaattag gatacttgac gaagcaagca
    22741 aaacaagcaa tcattgaaaa ttcaccagaa acgttggcac agacaatgga tgaatcacaa
    22801 tcacttctgg aaaagctgac aatcagcaat gattttctta atctattgat ccaaacagca
    22861 aaagataccg gggcattggg tgccaaatta actggcggtg gacgcggtgg ctgtatgatt
    22921 gcattagcac aaacaaaaac aaaagcccaa gaaatcagtc aagcacttga agatgcgggt
    22981 gctgctgaaa cttggataca aggattagga gtacatacct atgtttaaag gcaaagcacg
    23041 cgcatatacc aacattgctc taatcaaata ttggggtaag aaaaatgaag aactcatcct
    23101 tccaatgaac aacagccttt cgttaacatt ggatgcattt tatacagaga cagaagtcat
    23161 cttttctgat agctatatgg tagatgaatt ttatctagat ggcaccttgc aagacgaaaa
    23221 agcaacaaaa aaagtcagtc agtttcttga cctttttcgt aaagaagccg gcctctcact
    23281 aaaagcttca gtaatcagtc agaatttcgt tcctaccgca gctggattag cctcctctgc
    23341 cagcgggcta gctgctttag caggcgcttg caatactgct cttaagcttg gattagatga
    23401 tctctctctt tcaagatttg ctcgacgcgg gtctggttca gcttgccgaa gtattttcgg
    23461 tggtttcgtc gaatgggaaa aaggccatga cgacttaagt tcttacgcta agccagtccc
    23521 ttccgattct ttcgaagacg atttagcaat ggttttcgtt ttgatcaacg accagaaaaa
    23581 agaagtgtcc agcagaaatg ggatgcgtcg gacagtcgaa acatccagtt tttatcaagg
    23641 ctggttagat tccgttgaag gggatctata tcaattgaaa caagcaatca aaacaaaaga
    23701 tttccaactt ctcggagaaa cgatggaaag aaacggacta aaaatgcatg gaacgacttt
    23761 agctgcccag cctccattca cttactggtc tcctgacagc ttaaaagcga tggatgcggt
    23821 gcgacaattg agaaagcaag gcattccatg ctactttacg atggatgctg gccctaatgt
    23881 caaagtttta gtggaaaaca gccatttatc tgaggtacaa gaaacattta ccaaattatt
    23941 tagcaaagaa caagtaatta ctgcacatgc tggtccagga attgcgatta ttgaataaag
    24001 ggagattttt atgattgaag tatctgcacc aggcaaactc tatattgccg gagaatatgc
    24061 agttgttgaa acaggccatc cagcagttat cgctgcagtc gatcaattcg tgacagtcac
    24121 tgtagaatct gcacgaaaag tcggaagtat ccaatctgca caatatagtg ggatgcctgt
    24181 acgttggaca agacgcaacg gagaattagt tttagatatt cgggaaaatc cttttcatta
    24241 tatccttgct gctattcgct tgactgaaaa gtatgcacaa gaaaaaaaca ttcttttatc
    24301 cttttatgat ctgaaagtaa caagtgagtt agacagttca aatggccgga aatatggatt
    24361 gggttctagc ggtgccgtga ccgttgcaac ggtcaaagcc ttgaatgttt tttacgcgtt
    24421 gaatttatct cagttggaga ttttcaagat tgcagcacta gccaatttag cagttcaaga
    24481 taatggttcc tgcggcgaca tcgctgctag ctgttatggt ggctggatcg ctttctcaac
    24541 cttcgaccat ccttggctcc aagaacaaga aactcagcat tctatcagtg agttacttgc
    24601 cctggattgg ccaggtctat ccattgagcc attgattgct cctgaagatt tacgtttatt
    24661 gattggttgg acgggtagcc ctgcctctac ttctgatttg gtcgatcaag ttcaccgttc
    24721 gagagaagat aaaatggtgg cttatcagct tttcttaaaa aatagtacag aatgtgtcaa
    24781 cgaaatgatc aaagggttta aagaaaataa tgtaacgttg atccaacaga tgattcgaaa
    24841 aaaccgacaa ttactgcatg atttatctgc aatcactggg gtcgtcatcg aaacgcctgc
    24901 tttgaacaaa ttgtgtaatt tagctgaaca gtatgaagga gccgcaaaat cttctggtgc
    24961 aggtgggggc gattgcggaa tcgtaattgt tgaccagaaa tctggcattc ttcctttaat
    25021 gagtgcatgg gaaaaagcag aaatcactcc actgccgtta catgtctata gcgatcaaag
    25081 aaaggaaaac cgatgaatcg aaaagacgaa catgtttcac tagctaaagc ctttcatgat
    25141 aaacaaaaaa acgaatttga cttcgtacgg atcatccata accctttgcc gcaaatagca
    25201 gtttctgatg tcgatctaag tacacaggca gttgggttta cactaagcag cccattttat
    25261 atcaatgcga tgactggcgg aagcgaaaaa acaaaaaaaa tcaaccaaga tcttgcaatc
    25321 gtcgcaagag aagcagattt gatgattgcc actggttcag tcagcgctgc cttgaaagtc
    25381 ccttctttag ccgatactta tacgattatg cgacaagaat atcctcacgg gaaaatcatt
    25441 gccaacatcg gtgcagggac ttctgtcgaa agagccaaag aagctatccg attgtttcac
    25501 gcagatgcct tacagattca tttgaatgca cctcaagagt tggttatgcc tgagggagac
    25561 cgtgatttta ccaattggaa agtacttatt caagaaactc aaacagccat tgacgtcccc
    25621 ctcatcgtaa aagaagtcgg ttttggcatg acaagagaaa cgctcaatga tctagctgct
    25681 ttaggagttc atacagttga tatcagcggt cgaagcggaa caagcttcac ccagatcgaa
    25741 aacgcccgtc gctccaagcg ggaactgagt tatttagctg actgggggca atcgacagtc
    25801 tcttcattgc ttgaagcaaa tgaagcagac acttctatgg aaatcctagc ctccggaggt
    25861 atccgcaatg cctatgacat atttaaagcc ctttgtctcg gcgcaaatgc cgtgggtact
    25921 tcaggaaccg tactgacgca tctgatgaat cacggcgtag aagaaactat tatactgatg
    25981 aagcaatggc aagaagagct tcgtctgctt tatactatgg tcggcgcaac gaataccgcg
    26041 gccctacacc aacaatctct gatcttttcc ggaccagtga aggattggtg cgaagcaaga
    26101 gggatcgact tagtgaagta tggaagacgc acagaaaaga gcgtgggaca aaagtaaaga
    26161 acacttttgt ctcacgctct aaacacgtac aaacggcgga agtaaaagca actccttcgg
    26221 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaattcct tcttatttct
    26281 tggagttaaa tgcttctgtc ccagcctctt ttttcgaata aatgatgttt aatcagctgt
    26341 ccttcggtat tcgttcaatc cgctgatttc acaacctctc cactaaacag tgtactaaag
    26401 ctcttttaag gttgttttcc gatataggca agtatgcctc cgtccacata caagacatgg
    26461 ccgttaacga aatcagatgc gctagaagcc aagaagacag atggtccagc caaatcgatt
    26521 ggctcgcccc agcgtgctgc tggcgtacgt ccaacgataa attgatcgaa tggatgacgt
    26581 tcaccgtttt cttgtgtttc gcgtaatgga gcagtttgcg gtgtagcaat ataacctggt
    26641 ccaattccat tacattggat attgtattga ccgtattcag aagcgatgtt tttcgtcagc
    26701 atttttaatc cgccttttgc cgcagcataa gcactgaccg tttcgcgtcc taattcactc
    26761 atcatggagc agatattgat gattttaccg ccgccttttt cgatcatatc cggaataact
    26821 gcttttgaaa cgatgaatgg ggcatttaaa tccacgtcga ttacttggcg gaagtctgcc
    26881 gcatccattt cgatcatcgg tgtccgtttg atgatccctg cattgttgac tagaatatca
    26941 actgaaccta catcttcttt gatctgttga accatcgctt gaaccgcttc ttcatcggtc
    27001 acatcgcaga cataaccttt tgcatcgatg cctgcttctt tataatgcgc tataccttca
    27061 tcaactgatt cttgattcaa gttattaaat acgatttttg ctcctgctgt agctaaagag
    27121 cgtgcaatct caaatccaat accatagaca gcaccggtga ctaatgctac ttttccatct
    27181 aaacgaaaca tatccatact aaattccatc attttctctc ccttatgctt attttaattg
    27241 atccatttgc accatatcca tgtctgtata ggtgatattt tctccgcaca ttgcccagat
    27301 gaatgagtaa ttgcttgtac ctacacctga atggatcgac cagcttggcg aaataatggc
    27361 ttgttcattg tctacaacta aatgttttgt ttcatctggt ttgcccatca tgtggaacac
    27421 acgtgtatct tcattggcat aatcaaagta gacataagct tccatacgac gttcatgtgt
    27481 atgacaaggc atcgtattcc aagcgcttcc tggttctaaa atcgtataac ccatttgtaa
    27541 ttgacagctt tcgcatacat ttggatggat gtattggtag attttacgtt cattcagtgt
    27601 caggccttca ccagtctcca ttggttttac ttggtcgata ctgattttta catttggata
    27661 tttatgatga gctggaacag aactaatata gaatttagct gggttttcag catcttgaga
    27721 agaaaataca acatgtcgtg tttcttttcc gatatagtat ccgtcttgtt ttttcatttc
    27781 ttctttttgc ccatcgatct cgatgaatcc tggtccaccg atattgatca cacctagttc
    27841 acgacgctct aagaagtaat caacacctag ttctttgtct aatgtgattt ctaattcttt
    27901 ttcagtaggt gtcactccac caaagatcat tcgatcgttg tgcgtataag tcaaactgat
    27961 tttccctggt tcaaatacct tttctactaa aaattctttt ctcaattgtt ccgttgaata
    28021 atgacggata tcttctggac tgtgtgtata acgtgtttcc atttcttgca ttttcttcta
    28081 ctccttctat cgaatgattt ttccagagcc gcatgctaaa aattgtgcga cttcttcttg
    28141 actaaattgg ttgcagtccc catggatcgt atgcttcaag gcagaagctg ccgtagcaaa
    28201 atcaatgatt tcttgcggag acatattgga taaaatccca tggagcacgc cacctgcaaa
    28261 agcatctccg ccacctactc gatccacgat tggggtgatt ctatggactt gggattccac
    28321 atattcatgg ttcatccaca atgtcccctt caattcattt tcacttgctg aatgaacggt
    28381 acgcttggtc gaataaaaca cctgaatatt cgggaacttt tcctgcattt tttgataata
    28441 ataaattaga ggttgttctt ctgcttctgg cgctttctcg atacctaaca aataaagagc
    28501 atccattttt ccagctgagc agatatctac taaaggtaaa agctgatgga ttacttcgct
    28561 ggcttcttgc tgtgtccata attttccacg atagttgata tcgaagctga tcaggcagcc
    28621 agcttttttt gcagcttcta tcacctgttt cgtcattttt tgccattttt tagacaaagc
    28681 tggggtgatt cctgaaacat gaaatatatc tattccttgg aataactcct ctagcgacca
    28741 ttccaaatga tccatttcag caaaacttga tccggcacga tcatagatca ctgaggcggc
    28801 gcgttcgcca ctaccagttt ccatataata agtacctagt cttgatccac cagataatac
    28861 ctgtttcgta tctactccat aacattgcaa gtgctttttg actgcttttc ccaatgcgtt
    28921 atcaggtact tttgtagcaa aaatcgcttc atgtccgtaa ttagctaaag aaattgctac
    28981 gtttgcttcc cctccgccat aatgggcagc gaatcgttca cttgaatcga tacgggttcc
    29041 ctgctctgtg gacaatcgca acatgatttc tccgagtgtc actacttttc ccataagcag
    29101 ctacctcttg atttctttca tttttgctgc atactgttgc gcgacttccg ttactttgtc
    29161 aaaatctcct gtagctgcgg gtgctaaaag gttcccacct actccgactg ttacgacacc
    29221 agcatcgaac cactctttca tattgtccag actcacaccg ccagttggca tgatattgac
    29281 ttgaggcatc ggcgccttga aggcagaaat gatacttggt ccataagcgc ttccaggaaa
    29341 tagtttgacg atatctaccc cgcttttcag cgctgttttg atctcggtaa tagtcataca
    29401 tcctggtaag taaggaattt gatacagatt gcacatctct gccgtttctt gatcaaagga
    29461 agggctaaca atatactctg cgccagctaa gatagccaag cgagctgtga cagcgtctaa
    29521 tactgtgcct gcgccaatca ctatttctgg ttggtctttg tagaatgaca aaagttcttt
    29581 gattacttga tctgcttgag ggactgtaaa agtcaactca ataccagtta gaccaccttt
    29641 gatgattgcg tggcaagcat tcaaagcttc ttctttgctt tttccgcgta ccaccgcaat
    29701 cactccggcg tttttcaatc gagataaaat atcaattttt ttcatcttct ccacctattt
    29761 cctatttaga aattttattt tataacttgg aaatctatac ccatattata cggcagaaaa
    29821 gaaaaattgc aaacgctttt ttcattattt ttgaattctt tgctcgattt cgctttttgt
    29881 ttgcttgagg gcactgatta tttgttcttt tgtctcatct gttaaacgat atttcggcat
    29941 acttacacta aatgcaccaa tgatttgttc gtttttcacg attgaggctc ctatacaaaa
    30001 aatatcacgt tcgatttctt catcgtcaaa tgcgatgtgg gtttctttta ccgttttgat
    30061 ttcctttttc aaccgtaatg cattggtgat cgtatattct gtgtaaggga tcaacggatg
    30121 cttttcaaga taatcagtgt actgctcttc actgaattct gctaatacgg ctttgcccat
    30181 cgcagaattg tataatggtc gggtgattcc gatttttgaa gacatgcgga tcgtttgatg
    30241 ttttggatct agtttgttca cgtacaagat ctcattgttg tccaataccc ctaagtggat
    30301 agtctcatcg atcttttgct gcagctgttc taaaaacggt aaagtcaact ctaccaaatc
    30361 gatttgttca atgtttttat tcgcgtagcg aataagcttt gcacctaaac gatagttttt
    30421 ctctgaattt ttatttacgt agccgatcat caacaaagta tctagtattt tcaagactgt
    30481 agaagaggtc attcccacac cctttgcgat cgtctgcaaa gagacatctt ggttttccgc
    30541 taaatagtct agtattttag aggctttgat caatacagtt ccataaggct ttttattttc
    30601 catataatat tctcctattt ctttataaga aaaattatag cggaaatacc atcaatttaa
    30661 aagagatatc cagatataaa ctaccatttc tctttacagg caatttaatg ttgaagatcc
    30721 gcttcgtttt tacatcaaac tttgatataa agcaatgatt tcttctactg tcgtttctct
    30781 tggatttcct ggtgtgcata catctcgcaa agcatcttct gcaattgctg gaatatctgc
    30841 ttcttttaca cctaattcag aaatggtttc tgggattcca accgctttgc tcaaccgatc
    30901 gatctcacca caagccgctt ctcttacatc ttctaaagac atttcagctg ctcctttaat
    30961 ccctagaaca agagcgatct cacggtactt ttctccagta tattctttgt tatatttcat
    31021 gacagttggc aaaagtgctg cacacgctac gccatgagga atgttgtacc atgcacttaa
    31081 cgggtgagcc atcccgtgta ccagtcctaa accgacattt gagaagccca ttcccgcaat
    31141 atactgtcct aatgccattt tttctcgccc attttgatcg ccatctacag aagcttccaa
    31201 agaatggccg atgatttcga ttgcttttaa atgcagcata tcagtcattt cccaagcgcc
    31261 ttttgtgata aacccttcaa tagcatgcgt catagcatcc atacctgttg atgcagccaa
    31321 ttttttcggc attcccatca tcatatcgct gtcgatgaac gcgacgattg ggatatcatg
    31381 aggatcaaca caaacaaatt ttcggtgatt tacttcatct gtgatgacgt agttgatcgt
    31441 tacttctgct gctgttccag aagtcgttgg tatagctaat ataggtaaac atggattttt
    31501 cgtatcagca acaccttcta aactaatgac gtctgaaaac tctggattcg tcaaaataat
    31561 cccaatggct tttgccgtat cgattggcga acctccaccg attgcgatca aacagtctgc
    31621 ctctgcctct ttaacaaaag ccactccatc ttttacattt tggatcgttg gattaggtac
    31681 gatcccgtcg tataaagcat aagcgattgc tgctttatct agaagttctg ttacttttgt
    31741 tgctataccg tgctcgatca atcctttgtc agtaatgaca gctgctttgc cgaatcctcg
    31801 ctgctgaaat tcagggacga tcgactcaat cgctcctttt ccaaaatacg atgtttcatt
    31861 taaaatcatt cgattactca ttttctattc ctcctaattt gtattatttt tcacaataaa
    31921 aacgaaagaa ccccaagcaa ccggttgccc agttgcttgt tgctacagtt caaagactgg
    31981 catcaattcc caactgactg gagaggcatc tggatttgtc tccatgatat cttccatgta
    32041 cttccaccat ttttgattga tagatgtatt tgccattttt tcccataact tttcatcttc
    32101 gatcactaag taaccaaata acaaacttgt actcggatca agatagatcg aatagtcgat
    32161 gaccccgtgt tcacgaagtg ctgtacgcat ttctggccaa agttcatcat gtcttttttt
    32221 gtattcttct tgtttttctg ggtaaacttt catgcaaaaa gcttttttca ccatgtcaga
    32281 cacctgcttt cgtatgaact tcctccaaat aacctgcttt tggagtgaca ccaaacgcat
    32341 ctgctaaatg ccacaggttt tcgtccgtga ttgtttgcaa gattggtcct tgcgctttga
    32401 cgtacgtata gacttctgct gccttttctg ctgtctcgat caaaccaaag acttcatcca
    32461 tgtcttttcc tgacccgtag attccatgtt gtggccataa aaccaatcga gtttctttca
    32521 ttttttcagc tgttgcttcc ccaatctcat ttgttcccgg aaccatccac ggaatgatgg
    32581 aaattccttc tggaaaaaca actaagcatt ccgtacacat ttgccatagc gtacgactaa
    32641 aggaacgttc gtctaattca tgcgtaaaac tcatcgctaa tagatgacta gcatggcaat
    32701 gcataacgat ccgattatct gggtctactt ctaaacgggc gatatgactc atgaaatgac
    32761 ttggcaactc gcttgtagga aggcattcgt tttccagccc ccacaaaagt tccagtgttt
    32821 tgccatcttc agcaactcga acgattccaa gattctcggc aggttcgctc gctacatttt
    32881 taaaatatct tcctgaacca gttacaataa aatagcatcc tgctaaacgg ctggcatcaa
    32941 aaatcatcga gatgcggcgg atcacttttt caggatccaa gaacggtgtg atctcttctt
    33001 cttttagtaa ataagaaata ttcccgccat ttctttcatc ccatcccaag cgataaagat
    33061 tcgacgtcgt ttcgatcatt tctttcacat aaggcgcttg taaaatatct atttttttca
    33121 atatagccac actcctattt tcttaattcc aacacgtctt tttgatattc ctggacttct
    33181 tccagccaat ccaaaccaac tggaacttga tttttgtaac agaaataatt ccatacatcg
    33241 gcatacggga aatctttcaa ttcttccgta taagccattc ttgaagtaaa gtcaaaagcc
    33301 agctccattt ctttcaatcg ctcaactggt tccagcatag ctttcagcag tgctttttgt
    33361 gtatttctcg taccgatgac ccaagcagcg atacggttga tcgttgcatc aaagaaatcc
    33421 agaccgatat ttgttttttc caacaattcg ttacggacta attctttagc gatttcctgc
    33481 aattcatcat ccatcaaaac cacatggtct gaatcccaac gcactggacg ggaaacatgc
    33541 aacatgattc ctttgctgaa taaagctaat gaagataatt tattggaaat cacttctgtt
    33601 ggatgaaaat gtccagcatc tagacaaaca agtttgtcac gagtcaaccc gtaacccata
    33661 taaaattcat gggaaccgac tgtataagct tcagcgccta aaccaaatag tttgctttct
    33721 actgcatctt gtacataccg ttcatctaac tcttctgtaa aaatttcatc gagtgattcc
    33781 attaaacgtt cccgtggggt aaaacgatca atcggattat ctttcatccc atcaggtacc
    33841 caaaagttat tgatacttgt ttgcccagtt tctttcccaa agtactccgc gattttgcgg
    33901 cttcttttcc cgtgttcgat ccagtattca cgaacttctt tatctggatg agccaatgta
    33961 aagccgtctt tcatcattgg atgcgaaaag aaagtcggat tgaagtcaag accgatttct
    34021 tctttttttg cccactctac ccatttttca aaatgtttcg gctcaatttc atttagatcg
    34081 acagattctt ctgtatccaa atagatagca tgaaggttca atttgtgttt gcccggtatc
    34141 aacgaataag ccttctccag atcttgtcta agctcttcag gtgttcgtgc ggctcctgga
    34201 aaattccctg tcgccatgat tccaccggtc aattcgccat ccggtgtcaa gaaacctttg
    34261 acatcatctc cttgccagca atgcatagaa attttgatgt cctgtatctt ttctagtaca
    34321 gcatctgtat ctacaccgat agttgcatat ttttcttttg cttgttcgta atttctttca
    34381 atcgtgttca tacgattact cctttgattt tattcacgta ccattccatc gtgttttgct
    34441 tattttcatc tgggtaaaaa gtttcacatg gaaacgactg tttgattaca tttcttgctt
    34501 gttctatatt cgaaaactga cctgtactga gcatttgcat ggcaacattc ccgattgctg
    34561 tggcttcact aggtcctgct tctacaatga ttccggcata atctgctgtc aattgattca
    34621 gtagacgatt attcgatccg ccccctacga tgtgcagctt ttccaatttc gttccagtta
    34681 agtgttctac tttgatcagc tcttgtgcgt aacaaagagc aagattatca tacacacaac
    34741 gagcgatagc tcccgatgag acagggactg gctgctctgt ttcaaaacag tattgctgaa
    34801 tctcttctat catatttggg ggattcaaaa atcgagggtc attgatttcc atcacttgct
    34861 gcagcggttt ttcttttgca gcaagctctg ccaattccgc ataactgtac tcgtactctt
    34921 gcattcgggc aacttcctgg atcaaccaca tccccatgat gtttttcaaa aaacgcaccg
    34981 tgttatgggc accccattca ttcgtataat tttcagaaaa agcattgcga gaaacattgg
    35041 cgatctttgt ttcaatccct aaaagcgacc atgtcccact gctgatataa ccccaacgtt
    35101 ctccttgacc tggcgttcct aatacagctg acgccgtatc atgacttgct actgtgataa
    35161 aggtcacatc tggcaaatca tattttgaaa attgttcttt ttgcagatct cctagtatcg
    35221 ttcccggctc tgtcaatggt ggaaataatt ccttcgagac accgattttt tcaagaagtt
    35281 ctatttccca ttcttttgtc cctgcattca gcagctgcat agtagatgca ttagttttct
    35341 ccgtcaccat gttaccagta aagacatacc ctaaataatc aggtatcagt aacaatttat
    35401 gtgcttttgc caaattttct ttttcttcag caaacaattg gaacaaggta ttgaaaggct
    35461 ggatctgaat ccctgtctgc tcatacaact tatctagaga atagatttca gaaaactgtt
    35521 ctactgctcc attggtacgc ttgtcccgat aagcaatcgg atcatttatc cgatcccctt
    35581 gttcatcaat cagacagtaa tcgactgccc atgtatcgat ccccgcaaaa aggcgatcaa
    35641 tccctgtctg ctttaatttt tcaaatccaa tcaaaatctc tttgataaga taatcgatct
    35701 tccagcgatc gtgaccgtct ttgttccgaa agccgttttt gaaccggtgt acttcttcca
    35761 agatcaaaga accttttgca tccctcgtac ttttcattag tcgtccgctt gatgcaccga
    35821 tatcgatggc aacaaatgtt ggattcattt ctccacctct tttactttat tgttgattag
    35881 cactttatta ataaagcgtt atcatttatt gacaatgcta tcataataca gaatcttata
    35941 ttttgcttca cactattttg gtatataatt gcattatctt gacaagttgg gagtgtttac
    36001 atggattact gggaaaaagc caaccactca tggtcggaag actcaaaaag agtgatcttg
    36061 acgcctacac aaagaagcag gaatttattt ttttatatcc aagaaatcgg ttacttcaaa
    36121 gcaccaatcc cttactatac tgaacgagca catttacctt cttacttgat aaagtttact
    36181 ttgtctggca aaggttcgct tacatataat gataatcagc aacagctaaa agctggagac
    36241 ttatttttta ttgactgcca agaatatcag cgttatcaaa cgatcagcga agaaccatgg
    36301 gaaatggatt ggatccattt gaatggtcag aacgtccaat cattttatca ggagttcaca
    36361 aaagacggcc ataacacctt ccattcaaca ggagatccat cagaaaacaa gatccaccat
    36421 ttgatcacag aattgatcca catgcaacaa ggcacgaatg cccgtaccga tttccagtcc
    36481 tctgtagcta tccacgagtt gctgaatgaa ctgattttgc aaaagtatca gctagatttc
    36541 gaagaagagg atattcccgc ctacgtttac aaactgaagc aatacttaga cgaacatttc
    36601 aaagaaacga ttacacttga tcaattagaa caacaattct tattgaataa atatcagctg
    36661 agcaaagatt tttccaaatt tatcggttct ccaccaatcg attatttgat cagcaaaaaa
    36721 ctttcttacg ctaaagacct tcttcggtat acagaattta cggttcgaat gatttccgaa
    36781 gaaataggga ttgagaattt tgcttacttt agccgcctgt tcaaagcaaa aacaggacta
    36841 tcgccgagag actttcgtaa attcagctga ttcatataat atgtacgtca ttttttcaaa
    36901 atattttgaa atatatagat aaaaataaaa taaccgcagt ttctgcttct atgtaaacaa
    36961 cagcagctac tgcggttatt ttttagtgat cattctcatt gttcatcttc aaaatgttca
    37021 taatgaacca actgtttctg tgtatcacgt ttcaaaaatt ctttaggcgt tatgccgaac
    37081 tctctcttga atgctttcag aaaatgcgga taagaagaaa aaccactttt actatataca
    37141 gcactagagc tgcggtattc tcgcaacaag tgcttggcat agatcagctt tttcttcatt
    37201 acatactgat gaaacgtaaa tccagtgtac tttttaaatt ctcgtgtgat atggtatttg
    37261 ctgacaaaaa actgcttttc catttcctct aaagaaagcg gatgagataa attttttgag
    37321 atatattgta acatttgttc cacacgctca ttaggaacta cagcattctg ttcttgcgca
    37381 ctctcttctt tttgtacaac ttgattgaga aaaatcaagt attctaccag agcttgttca
    37441 tattttatgt cttcaccgta aattttttta tcaggaggat ggtcgataaa tgataatttt
    37501 tgcttcaata cttcctgttc tatttgcaat acacgactcc tcttctctcc cattggttga
    37561 aaacaagccg atagattaga ccatggcgtg gaacgacttt ctagaaaaga aggggtgaca
    37621 aagatataga cacgctcgaa tacatctgaa gattggcgga cgatacgatg caaatcattg
    37681 gaatggatga gcagcacact tcctgctttg atcgtaaatt gtcgtccgtt caaataaaaa
    37741 gtcgcttctc cctttaatgt agcatggatc tcatagaaat catgatgatg atagatcgtt
    37801 ttattttggc tgacgacatc tttgacatga tagatttcat actgatcaga aaccatcgta
    37861 ttgacttgtt caccgctgat tttcttttgg agcatgtgcc gcacctcctt ttgttcactt
    37921 atgcacaatt ttattatacc acgttttttg taatcaaaaa gagctgcttt tatatactct
    37981 tataaaaaag aagagacgtg gcatcttcta gccccgcccc ctccattcta aatattagtt
    38041 aaccaacgtc ttctttacat tattcaagaa gacttcttcc ataacgaatc ccatcatctt
    38101 ttgtttcttc aagaaccaca tacaggtacg gacgattctt catgatgatt tctttgattt
    38161 ttgtgtactc gatcacaccg tctcccaaat ttaccggcac gattttttcc tgttcgacac
    38221 gaaaatcttt caaatgaacc gcacatatct tttcaccaaa acgttcaaaa gcttcttcta
    38281 ccaattggac ttgttcttga taatcactac ttgtgatcaa attggtcgga tctaaaatga
    38341 ttcctagata gtccgaatct actgcttgga tcagctgctc tacccttgct aaagaataca
    38401 ccggatgatt caaacctggt tctatcccca ccatcatttg gtatttttct ccagctttta
    38461 ccaagcgacg gataacggac acagcttcag caaatgcttc atctgtgaaa ttttcttccg
    38521 tatattggat ttccggcaaa acacatccag tttccgaagc aaccatcgac gcaccaaagg
    38581 aagaagcata gcgaagatac tgctcgaact ttgtcagcaa ctcttgtcgt atcgttaaat
    38641 ctggatgaat catattgata tagcaactta aaattcctac ggtcacttct tgcttttcta
    38701 aaactcgttt gacataattt cccatcccag aattgatctc atttgcgcca gaaggtattt
    38761 caggaaacga aagacctaag gcgagctgca gtgtatgaat cccttgttct tttgtttttt
    38821 ctgcaagatc ttctatcgtt tggacttcgg aaagatcatg tcctctaatt gtaatgttca
    38881 acataactat tctcctaact cagcatattg ggtagccaca tgacgatttg cgggaagtaa
    38941 gtaatcaaaa acaagacgac taagataacc ccataaaaag gaagaagcgg tttcagcatt
    39001 gcctctgctt ttacgcctcc tacacttgca ccgacatata gtcctgtacc tactggcggc
    39061 gtgatggagc ctacacacag attcatgatt gaaaacaacc cgtaatgtac aggatcaacc
    39121 cctacacttg tgacgattgg taaaaatatt ggtgtaaaaa tcaagatagc aggcgccaca
    39181 tccataaaca tacccaccag taataacaca agattgacta ataacagaat aataaattta
    39241 ttatccgtta cgcccataat caacgaacta attgcctgag gaataccggt aaacgccatg
    39301 gcgaaagaca tcatggaaga agtagccaat aagaacatga tcgtccctga cattgctact
    39361 gattgaaaca acatattagg tatatctttt agttttaccg ttttatagta aaacatagca
    39421 atcaacaggg agtaaatgac agctatcgct gaagcttcta ctgctgtaaa tatccccgtc
    39481 aagataccac cgataatgat gacaatcaat agaaggctag gaatcgcctc ttttattatt
    39541 tttccagcat gagccatctg tcctttttcg acaacagggt atttattctt tttagcaacg
    39601 atgaatgcta ctagcatgat tcctagcgcc cacaaagcgc ctacgagata acctcccata
    39661 aacaaagcag agatcgaagc accaccagca acaagtgaat acatgataaa agcagtactt
    39721 ggaggaatga ccatacctgt cggagctgac gcgatattga ccgctgtcgc aaacttccgg
    39781 tcatatcctt cttccacttg ctgagggatc aacactccgc caatcgcggt agaagcggca
    39841 atggccgagc tagagatgga accaaataaa gcattcccta acacattcgt atgagccaaa
    39901 gcccccggca cgcgcccgac gaacaacatc gcaaaatcca ctagtttctt agctatccct
    39961 ccattattca tgataatgcc cgagagaacg aaaaacggaa tagcgatcag cgaaaaacta
    40021 ttgatacttc ccaccatttt ttgagctgaa ctaaaagcag aaacatcaaa cggtataact
    40081 aacaataaag tcatcatcga tgagaatgca atacttatag caatcggcat ccctaataca
    40141 agcaacacaa caaatacaaa aaataacact aatccggctt ctattgccat tttattcctc
    40201 catttttata ttttttcttg aattgctggc ttgttgctta atgtatcgat tagattgatc
    40261 aggctataga taatgatgag tacccccgat acaggtactg acagataaac atatcccata
    40321 ggaatactta aagaaggcga gatctgcccc attgtcaatg tgacagcatt ccatccgcca
    40381 tacaccatga tcatcgcagc aaataccaga aataaacttt gaacaacaac ttctaaaatt
    40441 ttactttttt tgccagttaa tttttcactt aaaaccgtta cggctaaatg tgctttttgt
    40501 cccacaacat acgcggaaga caacatggca aaccagacaa gaccgaatcg aacaagctct
    40561 tccgtgaccg tatttggatt tcctaaaacc gttctggaaa atacttgcca taaaacaaca
    40621 gcgaccatcc caacaagcaa aacagctccc aggatctcta ataccttgtt taaaatcatt
    40681 ttcatcacag ctacgctcct tgctcttgga tcttttgata aaggtctttg taggtaggat
    40741 cgtttttgaa tatatcatga agtggggcaa ctgcctctcg aaacggtgta ttatctacat
    40801 cattgaattg gactccatgt tcttcttgtg ctaaagcttt ttcttcttca attgcttttg
    40861 caaaagcttc aatttcaaaa accgttgatt ctttcgctgc ttcttcaatg gctttttcct
    40921 gctcatcagt tagctgttct ttgatggcat cattcatgat gatgatatct ggcacacgtg
    40981 tatgctcatc ataggaatag tatttagcca caccaccatg tccagctgta tacaagacaa
    41041 attcattgtt ttctgatccg ttgatcaaat tggattgaag agacgtgtac acttcatcac
    41101 ttcccatagg tacgggcgaa ccgcctaaaa gctctaccat tttgatagcg gtttcgcttt
    41161 gcatcacacg gatcttcttt cccttcaaat cttccggcgt atggatcgga ccgtctacca
    41221 tataaaagct gcgctgacca gaatcataat aggtcaatcc ggtaaatcct aaatttttgg
    41281 tcgattggta gattggctcc attatttctt cattatccat caccttaaag aaatgttctt
    41341 catcattgaa tagataagga actgaaaaaa tcgaataatc tttagagaaa ctttccaacg
    41401 ctgaggcact tacttttgtg aaatctactg ctcctgactg tgtcaattcg atcagttcac
    41461 gttctccacc cagttgtcca tctgggaaat actggatctg tacggaacca tctgtttttt
    41521 cttttactaa acgcccaaat tcattcattg catcaattac tggctggctg ttgcttgcat
    41581 aagcaaagcg caaggtaact atctcattta catctgcatc tgcgacagtt gccacactag
    41641 aattatttcc gcagccgctt agacctagca gacctaggct aagcgaactt gctgcaaaca
    41701 gcagtgatgt ttttttcata tgggtttcct ttcttttaac aataagagac gagcaattct
    41761 gtcaaacaaa gcaccgttaa cgactgtcca taaggcattg ctgtgattcc gatattccga
    41821 taaaaatcta aactgtcccc cattcctgtc ccgattgaaa cgttttgaac ttctcctttt
    41881 tcatcgattt gttcaagcaa tccttggata gcacgataag cgacttcttt atattcttgt
    41941 ggtaaataac gtttatggac cgctttcaaa ataccgtaag caaatcctgc agttgcagaa
    42001 gattctacgt aagaagaagg atcatccaac aacgtatgcc ataaaccgct ttcagactga
    42061 taagttttta atgcacgcac ttgtgcttca agtgtttcga tcaataattt gcgcaatgca
    42121 tcttctttcg ataattctaa gatttcaatg atctctggaa tagcaatcgt caaccagcag
    42181 tttccacgag cccaaaatgc ttcggcatag ttatgatttc cttcaaacgt ccagccgtga
    42241 taccacaatc ctgttcgttt atccatcaag tatttgatat ggatcataaa ctggtaacga
    42301 gcttcttcta aatattctgg gcgattcaac agtttcccga tttttgccaa aggaagcact
    42361 gtcatcatca atgtatcgtc ccacaactga ttcttatttt ccggtccata tgtggcatgc
    42421 tgtaacccgt cttcatttgt ccgaggcatg tcattcatga cccattctgc ccattgttct
    42481 aaataaggca aatagcgtga atctttcgtg tcttcataca agtaagccat agtcaatagc
    42541 ggtgccattg tattgacgtt tttgggcgga gcgccttctt ccatacgatc ctcaaaccat
    42601 tcattgacca cttgataagc gcgaggattt tttgtcatct ggtaattttt ataaatacca
    42661 tatagaccga caccttgtgg ccaattccac actgtccagc ttttgtcatc tactttcagt
    42721 ccatcaaagt ccaacaaaaa ttcaccatta tcgtctttga tcgttgtcag gttttcaacg
    42781 agcaggtcaa ttttttcttt cactaattct ttaggtacaa aaatctcttc tttaataatc
    42841 atctttacgc caccttttta aacgctttca ttttatatcc ttaatatagc agtaataccg
    42901 tccactataa atatcgcttt ttgaacaaaa actgtctaaa attgacctaa atgttaaata
    42961 acaaaagtaa ctttcagtta cttggaaatt aaaaaaagaa cttggagaaa caacgttttc
    43021 caagttcttt ttcatctatt ttcttatctt ttttctacgc tttcttttgc ttatccgttg
    43081 taagcatcaa tgatgatctg tttcaattca gagatcaatg gttctttcgg attagctgtc
    43141 gtacattggt cttcgtaagc taattcagcc atacggtcta cggttgtatc tagtgtttct
    43201 tgtgtgactc cttgagcttt tagactcatg ttgatgccga cagattttcc taattcataa
    43261 acagccgttg ctaaagcttc gactaattcc tctgttgttt cgcctttcag acctaagaat
    43321 tttgcgatat ctgcgtaatc tgtatctgca cggaaatagt catatttagg gaacatcgca
    43381 tgtttttgtg gatcttttgc attatagcgg ataatatgcg gtaaaagaat cgcatttgtc
    43441 cgtccatgag ggatcccgta ttccccgccg attttatgtg cgactgagtg acaaatacct
    43501 aggaatgcgt tagcaaacgc cataccagcc attgtagaag cgttgtgcat tttttcacgt
    43561 gattccatat caggtgtttt tactgatttt tctaaatagt caaagactaa cttgattgct
    43621 tgtaggctca atccacgtgt ataatctgaa gccatgactg aaacatagga ttcgattgca
    43681 tgtgttaaca catccatccc ggtatctgct gtgacagatg ctggaactga catgacgaat
    43741 tgaggatcga cgattgcgac atctggcgtc aatgcataat ctgctaacgg atatttcaca
    43801 tgtgtttcac tgtcagtgat gaccgcaaat ggcgttactt cagaaccagt ccctgatgtt
    43861 gtcgggatac atacgaattg tgttttttct ggtttgtcga tcttatatgt ccgtttacgg
    43921 atatccaaga atttttgttt tgcaccaaag aaagatgtgt ctgggtgttc atagaacatc
    43981 cacattccct tagctgcgtc cattgcagaa ccgcctccaa gagcgattac tgtatctggc
    44041 ttgaagtctg ccatcagttt cgttcctgcg tatacggtat ttgtcgatgg gttcggttca
    44101 acatctgaga agacttcgat cttcacatca tttttacgtc gtgccaatac ttcacgaact
    44161 gtatcgcagt aaccgaattg gaccatacca gggtcacaaa cgatcatgac acgttcaacg
    44221 ttttccattt tctctaagta caatagagag ttcttttcaa agaagatttt tggcggtaat
    44281 ttgaaccatt gcatgttatt tctccgtttc gctacagttt tgacattgat taagtttaca
    44341 gcagagacgt tacgagaaac agagttctta ccataagaac cgcatcccaa agtcaatgac
    44401 ggaatcattt cgttatagat atcaccgatg ccgccttctg cagatggtgt attcactaaa
    44461 atacgacaag ctttcattcg aagaccgaat ttgacatgca actcttcgtt ttctgaatgg
    44521 ataactgctg tatgtcctaa tcctccaaga ttcaacatac cttcacacaa atcaaacgca
    44581 tggtccgtgt tgttagcttt gatcatggca agaactggag aaagtttttc tcttgatagc
    44641 ggataatctg ggcctacgcc ttccaattca gcaatcagca ttttcgttcc ttttggtaca
    44701 ctgattcctg ccaattttgc gatttccatc gcagaatgtc caacgattga tggattgacc
    44761 gcatattttc cttcgttcat cactgcatct tctaattttt gcaattcatc tggttttacg
    44821 atataaactt ggtgtgcttg gaattctgct tttactgctg catagatttc tttatccacg
    44881 atcacagctt gttcagaagc acaaatcatt ccattatcaa atgttttcga aacgatcaaa
    44941 tcgttcactg cacgtttaat ttttgctgtt ttttcaatat aagctggtac gttacctgga
    45001 cctacaccta aagctggttt tcctgtagag taagcagact tgaccattcc agctccacct
    45061 gtcgctaaga cgatcgcgat acctggatgg ttcattagca tagaagtcgc ttcgatcgaa
    45121 ggatgttcga tccattggat acaattttca ggagctccgg cagcgatagc agcatcacgt
    45181 accactcgtg ctgcttctgc agaagatttt tgggcacttg gatggaaagc aaaaacgatt
    45241 ggatttcttg ttttcaacgc gatcattgat ttgaaaatcg ttgtggaagt cggattcgtt
    45301 gttggagtta ctccgcaaac cacaccgact ggttcagcaa tttcaatcaa ccctttttgg
    45361 acatcttcat tgatcacacc aactgtttta tcatgtttga tattatgcca aatatattcg
    45421 gacgcataca tatttttgat tgctttatct tcgtaaacac cgcggcctgt ttcctctaca
    45481 gccatcttcg ctaatggcat atgttgatcc aaagctgcca ttgccatttc atgcacgata
    45541 tggtctactt tttcttgatc aaagctttcc atttctttca atgctgcatt tgctttttct
    45601 gccaaatcat caatcattgc ttgtacgtca attgtcttcg ttttttcttt ttctattact
    45661 ttagccatca ttatcctcct aatgggtcat ggttatttgt taaataattc acatgtatat
    45721 catacaatat ttatttttct atgtaaacac ttttttgtga tttttttcac agttattttt
    45781 aaatataaaa ttgctttatt tacaggtgtt tgtatagaaa aatatataaa gttatttatt
    45841 attatttata tgacacaccc gattattcaa tattgagcaa tatatcccat gctcttatct
    45901 ctttcttttt tgaataaaaa atgaggtagg acatctttct gccctacctc gtctttaaat
    45961 aagctttgat acttattttt cttccttctc ttctgtctct tccagcgttt ctgttgtttc
    46021 agtttttact tcaactgtct cagctggttc ttctgttgtt tcaacagtag tcgttgcatc
    46081 atttgttacg acagtccctg gtttgaccgt tttgattgct gcacgatcgt actcaaggaa
    46141 gatcccttcg cagtctaaaa caactgtccg tttgtcattg tcgatttctg aaacaactcc
    46201 atgtaatccg ccgatcgtta cgactgaatc acctggtttc atgctatcta acaagttttg
    46261 acgttcttgc tgttgtttct tttgcgaacg tgacataaaa aaccacattg ctaatagcgc
    46321 gataaacatc acaagcattg gtaatgttcc catttttttc acctcactaa ttctgtatct
    46381 acaaaaaaca ctttagcaga aaaatgctga taacactagt tttttactta atttttaaaa
    46441 gattttcctt tagaagcttt ttgcgttttc tttattgaac ccgtattctt caaaaaaggc
    46501 ttctctaaat tcaagcaaat tatcatccat gattgcttga cgtacttgtt tcattagatt
    46561 caacaagaag tataaattgt ggtaagaagt caaacgaatc ccaaatgttt catcacattt
    46621 gatcaaatgg cggatatagg cacgtgtata gtttttacat gtataacaat cacatttctc
    46681 atcaattgga cggaaatcat gggcatattt cgcatttttc actactagac ggccttgaga
    46741 agtcatacat gtgccatttc gagcgattcg agtaggcaag acacagtcaa acatgtctac
    46801 tccacggata acaccatcga tcaatgaatc agcagcaccg acacccatca agtaacgtgg
    46861 tttgttttcg gggatcaacg gtgtagtgaa ttctaataca cgattcattt ctgatttagg
    46921 ttccccgaca gataacccgc cgattgagta accagggaaa tccatcccga ctagatcttt
    46981 cgcgctttga cgacgaagat cttcaaaccc tgctccttgg atgattccaa ataatccttg
    47041 acggtctgga ttagcatgag ctttcaatcc tcgttctgcc catcgagacg tacgttctac
    47101 tgattttttc acgtagtcgt aactttcatc gaatggtgga cattcgtcaa aactcatcat
    47161 gatatcagag cccaatttat tttggatgtt gattgctttt tctggtgata aaaacatacg
    47221 cgaaccgttc aagtgatttt tgaaatgcac gccttcttct tcgatattcc gcatatcact
    47281 taatgaaaaa acttgaaagc cgcctgaatc tgttaaaatc ggttgatccc agttcataaa
    47341 tttatgcagt ccgccagctt cttctactaa gtcttcacca gggcgtagcc ataaatgata
    47401 tgtattactt aaaataacgc cagcacccat ttctttcaac tcttctgggg acattgtttt
    47461 tactgtagct aatgttccta ctggcatgaa cattggtgtg gggaaagttc cgtgtggcgt
    47521 gatcaattcc cctaaacgcg cgccagtatg tttttctttc ttgattaaac gatagcgaat
    47581 ggcaggttgc gtcattcatt tccctacttt ctatataaaa tatcttgaca actcccacca
    47641 tcataatcgt ttcaagtgct ttttgcaagt tccctcggct tccttctact ttatttaata
    47701 ttcttttttt tatccttttt ttatgaataa caaaaaaaag aaaaacatta tgacagaaat
    47761 attttttgat acatggtata ttggtgttat taaagaaaag gagggaaaaa tcatgcaatc
    47821 ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa ggacgttggg gacaagctgt
    47881 tttactaaat ctaatcccta cattgattgg tattgctact attcttctta ttatgattcc
    47941 tattatcttg tttgccacca ccatatatga tggatcagcg agcgtctcag aagcgacaac
    48001 ttcaggaggc ggtagttcag gaggcggtgg cggaattatt tccagcatca ttagcgcttt
    48061 gttcatgagc ggtatttctt ggacttatct tgatatttta cgtggacaaa aagatcagat
    48121 cgaaccattc aaagatgcat ttcgtggatt caaaggtttg tttatcggcg gagtgatcag
    48181 cttagctgtg ctgatctcga tctttacaac attatggggt ttgctattag ttattcctgg
    48241 aatcattaaa agctattctt attctcaaag ctattttatt tactacgata tcgtccaaga
    48301 aacaggacaa acacctaaag tactggatac gatcactgca agtcgaaaat tgatggatgg
    48361 ttataaagga caacttttct ggttagatct ttcatttatc ggctggcata tcttagcatt
    48421 agcaactgct ggtattggtt atctatggct cagcccatac atttctgcaa caaaagcagc
    48481 attttatgaa caattgccaa aagatattta aaagagagtg tgacttgtga ccgagggggt
    48541 ggcaggagaa caccgtttat cgagaggcag taaaagaaac attcagccca ttagcaaaaa
    48601 ggaggtgtga caacattcat gtcacacctc ctttttgtat agttccagta aaaagaaaat
    48661 caacttagaa acagttaatc attcctcttc ttctgcttcc ggtaatcgat ggaacttgat
    48721 cagttcattc accgcgattg gtacgataga taaaatccct accaagaacc agtgattcaa
    48781 actgatgtgt actaggccga atagttcttg cgtaaatggc agcaaagcaa cagccgttgt
    48841 aatagctaat gccagtaacg ccatttctac caaagcttta ttggtcgcta aattgacttt
    48901 gaagatcgat tggctgctgc gtacattgaa cacatgcaga atcgatgaat aagctaagat
    48961 caagaatgca acagtttgac cgacatgctg acttgcgtct acccatgggc tgatagtatc
    49021 gacataacta cctaaataat aaccaaacaa ggttacgatg gtgaagaccc ctgcatttat
    49081 ggcaattttt tgcaataaac ctcgagcaaa gatcccttca tttttcggaa tgggtggctg
    49141 atccatgatg ccgtgttccg ctggctctct tcctaaagca aatcctggaa gtccatcagc
    49201 aactacattg acaaacagta gttgcacagc tgtaaatggt gctccccatc ctaacagcat
    49261 cgcaatcaaa acaataaaga tctccgaaat attacagctt agcaaaaagt tgatagcttt
    49321 acgaatattc tgataaaccg aacgtccttg actgacagcg tctacgatcg tcgcaaagtt
    49381 gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca tctgttcctg tgatccccat
    49441 ggcacaccca acgttacttg ctttcaacgc tggtgcatca ttcacaccat ccccagtcat
    49501 cgctacgact gcacccgtac gttgccaaga tttgacgatc cggatcttgt cttctggcgt
    49561 tacccgagca tagacagaga gagctttgac acgcgcatct aattcttcgt ctgataattg
    49621 atgcagttct gatcctgaaa gtgcttctga cggatcattc aagataccta attctttcgc
    49681 tatggcactg gcagtcacca catgatctcc agtgatcatg actgttttga tgccagcctt
    49741 tttcgctcgc ttgattgctc ctttactttc tggacgtggt ggatcgatca ttccgatcag
    49801 accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa atatcttgcg gttcttcgtc
    49861 ataagtagca taaccaacag caatcactct cagtgcccgt ttaccaaagc gatcattaac
    49921 gatcgccgct tgttctacat cgcctgaact aaagcgcgga gcaagaacat cgaatgctcc
    49981 tttggtgatt gagatgtatt tttcgcccat ttggtgaact gttgtcatca tttttcgttc
    50041 agaatcaaaa ggaagctcgt ttactcgtgg atatttttct tctaattctg cctttgtatg
    50101 ataattctct tcaacagccc ttacgatggc tacttctgtt ggattacctt tgataacaag
    50161 gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt gcagccattt tcaatatttc
    50221 catcgcgctg ttcgtcattg aatcttcgat attcgttact tcatcttctt tgctccatac
    50281 ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt ttgtctgagc agataacatt
    50341 agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg atcgcatgtt tcctcgccat
    50401 cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca gtcaaggttt ccggtacagc
    50461 tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg agtggttctc cttgcagttc
    50521 accaatcaca aatactaaag ccgcagccgc taaagcgatc atactgattc gtttgcctag
    50581 ctgattcaat cggacctgta aaggtgtttt ttgcatagta ttttcgttca aaagatccgc
    50641 gatcttaccc atttctgtag ccatgccaat ggcagtgacg atcgctttcc cacgacctgc
    50701 agcaaccgta catcctttaa ataccatatt aagttggtca cctaatgact catcttcttc
    50761 agcaataaat gctgcatctt tttcaactgc ttcactttct ccagtcaacg ccgattcttc
    50821 tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca gaaatagcat caccggactc
    50881 taaaacaaga atatcaccca ctaccagttg ctctgactca acagtggtca ctttgccgtc
    50941 tcgtatgaca gttgccgttt gtttattcat atcttgcaag gattccagtg ctttttctgc
    51001 gtttccttct tggacaatcg ccaataccga gttgatcaca acgatagcga tgatcagcag
    51061 tccttcaaat aaatccccat gttctgtagc aaatgcggta taaaaggaaa tcaccgctgc
    51121 tacaagaagg atgatcgttg taaaatcgga caagcttcga aagaattttt tgatcatcga
    51181 ttctttcggc gcctcatcga atttattcaa tccctgcttt tttcgccgtt catccacttc
    51241 ttgtgaagac aacccagtat ctggatctgc ttctgttacc ttctttactt cttctattgt
    51301 ctgcttgtat tcttccatcc tattcctcct ttaaaatctt cgtacaaaaa aaggtactta
    51361 ttttactata acatcctttt tttatttact tcaaataaag ggcttatcga ttatttgtta
    51421 agaaagcgta aaatttaaag tgcttatatt tgcaaacgct accaaaggat gctattatat
    51481 agatgtaagg aaggaggata ccatcaaata gacagaagaa tttctcgata gaacagtgta
    51541 agcaaacaaa gttgttagaa gtacaggttt cacccagaca tgcgtgtaga gtacaaacct
    51601 ataaatagtg aatgcgtatg gatgtttgga tcacgaagta tccgaaacaa gaagtaatgc
    51661 gatacgtaac aattgaataa cgtaaacgtc tgaaaattga ataggaacaa gagatgtttc
    51721 aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac atgaagaaat tcaacaccgt
    51781 gctttgcatg tatgatggaa agttacaatc atttctgttt atttgaatcc ttccgaggta
    51841 caagaaagcc gagcttatcg agagctcggc tttttctttg tcataaaatc tttgtaaagc
    51901 ttttttgtta ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc
    51961 aaaactaaag aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc
    52021 tcttcctgca aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt
    52081 tgagaaagct tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc
    52141 gctgtctgct ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga
    52201 agttgttccg acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc
    52261 cgcttcttcc gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac
    52321 gctgacaggt cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc
    52381 ctttgcttcg atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc
    52441 agcagaacca ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc
    52501 tttgatatat ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc
    52561 ttcatactca aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctactgcttg
    52621 caatctgcca tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggtttgat
    52681 caatgtctcc cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc
    52741 agtatctggt ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag
    52801 cgcatcacca ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc
    52861 agttttccga tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc
    52921 gattaattct tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac
    52981 tttctatcat gtgcttcatc attctatcat gtttttcgtt ctgttggcaa aatcagcact
    53041 ttttcacttc tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat
    53101 tgttttaggc atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc
    53161 atgattgaat ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac
    53221 gtcaatcttt catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga
    53281 aaaacgacga cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg
    53341 attgatggaa aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat
    53401 gttatccaaa gtattggatt gatgcctcac atgacgatcc gtgaaaatat cactcttgtc
    53461 caaaaattgt tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat
    53521 cttgtggaat tgccccgtga gatgttagat cgctacccac atgaattatc tggtggtcag
    53581 cagcagcgta ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac
    53641 gagccattcg gtgcgcttga tccaatcact cgtgattcct tgcaagacct tgtcaaagat
    53701 ttacaagagc gtttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt
    53761 aaactagcaa gccgtatcgc catcatgagt gaaggccgtg tgatcaattt gatacaccag
    53821 aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga gttgattgga gaagatcgcc
    53881 tactacaagc caagccaaat gcgacaagag tcggcgaagt tatgctaaat acagcaatca
    53941 cgattacacc ggaaaaatca ttacaagaag caatcaaact gatgagggaa aaacgtgtag
    54001 atacgttgtt agttaccgat aattcgaatg tattaaaagg atttatcgat gtagaaacgt
    54061 tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat tttgaataaa catgtttttt
    54121 acgtaaaaga atccgcctat cttcgtgata ccttgcaacg cattttaaaa cgcgggctga
    54181 aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg tatcctgact cgtgcatcat
    54241 tagtggatat cgtctacgat gtgatttggg gagaagatcc cgcaactact gatgttgcag
    54301 agtcagctac tcctgaagaa tctaaggagg tctgacctat gcagcagttt tttcatcagc
    54361 acggttcaga aattctctca aaaggttttg aacatcttta tatttcagga cttgctttga
    54421 tcattggtat catcattgct gtaccgcttg gtgtcttact gacacgtttt ccaaaagctg
    54481 ccacgattgt gattggctta accagtgcct tgcagacaat cccttctctt gctttattgg
    54541 cattgatgat cccgctattt ggcgtaggta aattacctgc gattatcgca ctctttattt
    54601 attcattatt gccgatttta cgtaatacgt acattgggat gaaaaatgtc gattggaatt
    54661 atcgggatgt ggcaaaaggc atggggatga ctgagttcca atccattttt tccgttgaat
    54721 tgcccattgc tatgccaacg atcatggctg gtattcgttt agctgccgtt tacgtgattg
    54781 catgggctac acttgcctct tatatcggcg ctggtggttt aggcgatttg atttttagtg
    54841 gactgaataa ttaccaacca gatctcattt tggcaggtac gatccctgta acaattttag
    54901 cactattggc tgattggctt ctaggtctct tggaacaccg gctgacaccg atcgcactta
    54961 gagaggagaa tgaagaatga aacgtatact aaaaatgacc atggttcttt tctctcttct
    55021 tatacttgcg agttgttcct tccctggtct tgccagcaat acagatgaag acaccatttc
    55081 gatcacaggt ggaatcactt cagaagcaca gatcttagca agtctagttg ccggaatggt
    55141 tgaacattat acagataaaa ataccgcaat catcaacaat ctggctacta caactatcaa
    55201 ccatcaagcg atgatgaacg gagatgcttc aatttcagcg gctcgctaca caggaacaga
    55261 tttaacgact acattgaacc ttcctcctga aaaagatccg aaaaaagcgt ttgctaccgt
    55321 gaaagatgaa tttgagaaaa gatacggtca aacgtggttc ccgtcctata gttttgaaaa
    55381 tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa taccatctat ctaaagtcag
    55441 cgatctgaaa aatgtagcgg atgaattagt agctggtgta gatacttctt ggatcaatcg
    55501 taaaggagac ggttatgacg gcttccaaga aacttatggc ttctccttca attcgatatt
    55561 acctatgcag attggacttg tttatgacgc agtcgaagca ggaaaaatgg atattgttct
    55621 tggctactct acggatggac ggatcgccag ttatgatcta gtcatgttgg aagatgatcg
    55681 tcaattcttc ccaccatatg atgctgctcc agttgtagac aacaagatat tagctgatac
    55741 gcctcattta aaagaagcct taaataagtt aggtcatacc atcagtacgg aaaaaatgca
    55801 ggaattgaac tacgaagcag ataataatct agtggaacca tcagtagtag cacaacgttt
    55861 cttacaagaa aaccactatt ttgaagggaa gtgaagcaat tggataaaat gaatgtcttt
    55921 caacaatttt tctattattt ccaggaaaat ggctcctata tctttgcaca atttatccgt
    55981 cacttcctga tttctatcta tggtgtcctc tttgcagcag ttgtcgggat tccggttggg
    56041 atcatgattt ctaggagacg taaattagca aactgggtga ttcgtttggc gaatatcatc
    56101 cagacgatcc cttctctagc catgatctcc attttaatca ttggattagg cctgggagtc
    56161 aatgtcgtga ttgttactgt atttttgtat tccctcttac cgatcatcaa gaatacgtat
    56221 accgggatga tccaagtaga caaaaacatc cttgatgtcg gcaaaggcat ggggatgact
    56281 gctagacaac gactattcat ggtagagtta ccactctctg tttctgttat catggcaggc
    56341 ataagaaacg cgttagttgt tgccatcggg ataaccgcga tcggtgcatt cgtcggtgct
    56401 ggtggtcttg gagatatcat tatccgagga accaatgcaa cagagggcac atcgattatt
    56461 ttagcaggtg cattgccaac tgcattaatg gcgatcatca cggactggct tttaggtatt
    56521 ttggaaagac ggctagatcc agccagccgt acatcacgct gatgttattg taaaaaacaa
    56581 aagcttctgc gaccatttgt tggtcgcaaa ggctttttta gttctgtcat ttttaaggca
    56641 ataatccact ctgattcttc cctccttatg ggcaaagagt tgcttttctt agctatttat
    56701 ccattccgta tactccaact tcatactgcc aacgggttca tatctgcatc caccgactaa
    56761 gtttttttct tatccgctaa ggcattttgc caatccattt tcccatcact cgtcatggaa
    56821 ttcataccac atgatctcta aatactcgtc tgcttgttct ttgaattggg catcaattct
    56881 tacatccttt ggcgtggctt tcatttaagc cttttccatc agtcctcgat tgttcgatgc
    56941 cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt aacttcgatc atcttttata
    57001 caatcgggaa cttgccatcc tcatccttgg ctaaaaacat atctagccat tccagcttct
    57061 cgccatggga ggtttgcggt tcctaaaacc ggtacacgat tcttatggac tgcatccgtc
    57121 gcaactagac ttggcgatac gatgatcctt caccagaaga gcacccccgt aaaccaattg
    57181 atcgatatat tgccaataag aaaagacatt ggcgtaaaag atatcgatcc ctcgtgatgc
    57241 attgccgttg atactgctat tcatggccga aagcgccacg actttaactt cttccgtttg
    57301 agtgacattc atgttcggca atgtcgcttt ggatacccgc tctgctaaag gaacgacact
    57361 ggtattgtat ttcgtatctg tatcttttcc aaaagaccat gccaacgaat cctctgggaa
    57421 ccaataagac gactccggtt gattgtccat tgcttttttc aacatacttt tggctgtttc
    57481 tcgataaacg acatttccaa ttgtcttcgc ctcactttgg cgataatcga tggtcgttgc
    57541 cactgccaac actaacaacg atgctccaat tttactgatt ttccttgtca cacttcttcc
    57601 tccttgtttt acataaaccc gtcaacattc cattcctaaa ctcaaacaat gacccttcaa
    57661 aggtggagat ataggaacaa attaaagaaa tcatgcaata aatccaagaa ggttggtttt
    57721 agaacaaaaa aagagtctcc tttttgggga gactctttgt taactatcta aaaaggttgg
    57781 ctcttctcac ttcactcata atgtgtccag gcagaataaa tttctacaac tttattttcc
    57841 tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc tgcgtgagta tctgccttca
    57901 tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat atggatcttc cttcaatgtc
    57961 tgaatcactt cttcaaattg tttctttaaa ttcgtttgct tgatttttct taaatccact
    58021 ttcgcagagt ttttgattgc gactgtatag ttactcatca aagagcttcc caatcaatat
    58081 catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc tcgtacttta tctaaagtac
    58141 ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca gtcttccagc ccaatcaata
    58201 cagcattgtt tccactacga tcactaataa tctcgatttc agtatgattt tcatttacct
    58261 ctttcaataa ctgataaaaa tttttacgag cagtgctagg attcatcgtt gtattagcca
    58321 ttgtctctcc tcctttttct gatataatta tagcgtactt gattaagtac gtcaaaaaaa
    58381 tacaataaag cacttaaatt cagcaaactt ttcctctttt ttacatagag aaaaagaccc
    58441 ctttttaggg aggatctttg tccacgctca cttcttactc ctttacgact agcggtagcc
    58501 aaacgtaaag taattggtaa caacgtatca tctttagcaa aggtcgaatc caaagcctgt
    58561 gagtctatgc aacaacacca atacacgaag gttcctcgtt gcagtgtgac ttgattgata
    58621 tcttgaataa cctgaggata cgcacgaaag atttgtaacg gttattcaaa actgattttg
    58681 gccgtcaagt cttctactgc ctcaaaaatg attgcctctt tcgtctcctg tcattcatgt
    58741 tcactatcga tttgatactt gcttagaccg ttgggtttgt gaatcaccaa ttaaaaatgg
    58801 cactgagtta tggtaacaaa aaatggtcac ttgacgaggc tgagattcct acttatgaag
    58861 acacatggca cttaagagtc gaggtaaagg aagaagctga aaatatagga ggctgtacat
    58921 tatttggcaa aggattcgga aattatgatc aagacatcaa aatcaaaatg ttttacagct
    58981 aaataggaag aataataaca tttcatacgc tgttcccatc tttaatggct tatagagggc
    59041 acctttagct gagaataaga caaaacagac cctctagctt caattaagag ctagagggtc
    59101 tgtttttgat tttaaaactt ggtttatttc ttcaaatggt agctataggt gctgacaacg
    59161 atattttcac gccaagacaa tcgcaatctt aaccgcaaac ttgtttggtt atggagaata
    59221 ttctgccagc gtctgtttgg tacaaattga ggaatcaaca cagtagttgt atagttttgt
    59281 ttcgctgcat ttttacttac gatatctaca taacggataa ttggattttc aatcgagcga
    59341 taagaagagt ggacgattga aaaacggaca tctgggaaat gctttttaaa ttctgcttcg
    59401 atttcttttt ctttttcgac atcttcgtct agcgaaacat gcatcgcaac gacataatca
    59461 ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc gtgtcacatt cccgactaat
    59521 acaagaacgg tattcccgtc ataatcatgt agttttgcat cattttctaa acgaagctgt
    59581 tctgctactt ttttataatg atcatggatc ttataaaaaa tgaacataac gattggcata
    59641 atgatgaaga aaggccaaat gtcccctaac cgatagacaa acaaaatcgc aatgattgca
    59701 tatgagatga acgcacccgt aatattggca atcgatttgc ttaaccatct ttttccttct
    59761 tttttccatt tgacgaccat ccctgtttga gataaagcaa atggaatgaa cacgccgata
    59821 gagtataacg gaatcaagcg ttctgttgag ccatggaaaa taaacagcaa tacgatcgaa
    59881 cctagcgcta gtgtgataat cccattggaa tagcctagtc gatccccacg atcttgatac
    59941 atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa gaactggaaa agcagaaaaa
    60001 ccggtgttag cagcaactgc taaaatcagc gctgtagcaa attgaaggac gtaatacaaa
    60061 ataccatgtc caaagacagc tttaccaatt tgagacaaaa cagtgacttc tttttcagga
    60121 acgatcccat accaatagtt gataaatgtg atccccacga agaagaaacc taagatcaat
    60181 gccatcatcg tcaatgtagc agcggcattt tttgctcttg gcttcttaaa gaatggtaca
    60241 gcattactaa tcgcctccac cccagttaaa gaggaagaac cagaagaaaa tgctcgtaaa
    60301 atcaaagcga tagaaacacc cggcacagcc gctcctggaa gtgcagtagc ttgcaatggc
    60361 acagcacctg tcacgatatt atataaacca acaacgatca gtaacgagat cacaaggata
    60421 aatgtgtaca ccggaaataa taggaacgat gcagattccc tcagcccacg aagattcagc
    60481 atcatcagta gcaaaacgat tgtcacagaa atagcgactt gatgaccgta gagtgctggc
    60541 accgctgatg tgatcgcttc agcacctgct gagacagata ctgctactgt caacatgtag
    60601 tcgatcaata aagagccacc ggaaatcaag ccagcattct ttccaagatt ctcactgctg
    60661 acgacatatg ctcccccgcc atgaggataa gcatggatga tttgacgata tgatagcgtt
    60721 aatgaaatca ataaaataat aacaaaagcg gcaatgggta gcgaatacca gatggctgca
    60781 gcagacaaag cgactaaaac gacaacgatt tgttccgtac catatgcaat agatgataac
    60841 gcatcagacg acaataatgc caaagcagca aatcgggtta atttgtgctc atcattttca
    60901 gcagatttta aaggcttacc tactaacaac ctttttaagt aatccaattt ctaaactcct
    60961 ttacacacaa ttttacttca agttttatag tcgaaaattt atagtaaaca agatgttata
    61021 gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa ataattggaa aaaaacagaa
    61081 aaatatcaag cgctttttca aacgctttaa tacagcaaaa atctaacatt tttttatggg
    61141 ctttttcttt gaattctata ataaaaggat agaacttgac ttagaaaatc aagttctatc
    61201 ctttttgata tcttaacttt tggaagagag atgtaaggct gctttctacc catcattctt
    61261 actactcgaa gcatagcgca gttgtcacaa cctcttgata aacggtgttt aaaagcttgc
    61321 atctgcggta ataagcccaa ccaaattaaa aacataagaa gctgttttta tttggtcgtt
    61381 cttattactt gatgcagagc agctttttta caacctcttg gtacacggtg ttcaatcagc
    61441 tgaccctcgg tattagctca aaaaaacggc aaaatatgag gaactatttt tccatttttt
    61501 tctctaatac tcaggtcaaa acactgattt cacaacctct ttttttaatg aaaaatatgg
    61561 ctctcatgcg ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc
    61621 gttcctacga ttccaaagac aacaaacaaa ccgatcatga tccagattgc tttcgtagca
    61681 tttggccaat aaattcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc
    61741 aagaacggat tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct
    61801 ccgccagaga tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat
    61861 aatgcagcca agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac
    61921 acgctgtaga ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg
    61981 atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct
    62041 cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt
    62101 gaaagaagaa cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa
    62161 ccgttatttg cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag
    62221 tcactttctt ttgttgcgtc tagtttcaat aatttgatga cttcgcctag atcgacttct
    62281 tgttcgcctt ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag
    62341 tcattgttga tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca
    62401 tttactgttg tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata
    62461 aaatcagcgg ccaatcctaa tttttgctga atgatcggta attcattgtt gtataggtct
    62521 gccccttcat tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca
    62581 tcatggattt cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact
    62641 gcattcgtta cagttgcttt cgttgagctg agaagcgaat caaagtcgat ctggtccgct
    62701 ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga cttggtctag tagagaagtg
    62761 acagtggaac tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca
    62821 acggcatcaa tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct
    62881 gtgttcaaag cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc
    62941 tgatcaataa ttggctgtaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac
    63001 tcgaccagtt tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat
    63061 acattgctga tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt
    63121 acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc caattgactt caatgtaaca
    63181 tctacactgt ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt
    63241 tggtttgcta gatcacttgc ctgattttct aaatttttca catctggcaa gatatcttgt
    63301 acttgttgga tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct
    63361 tggatcgaag caaaatcgcc gtcgatctct gctagttgct taccggcatt ttggatctct
    63421 gggatttttt cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg
    63481 tcattcaatg caacgacttt ttcacccatc tcatctactt tagggatgta atccacgaat
    63541 tcatttgctt tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact
    63601 tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatcttta
    63661 actttattga tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc
    63721 aataaagtcg aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt
    63781 gctcctttat cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg
    63841 atttttggtt tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt
    63901 ggcaagtaaa tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt
    63961 gaatcaacga attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc
    64021 ccaatggcca cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt
    64081 ggcagctctc cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat
    64141 agcgaaggga tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc
    64201 cgtttccagt caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc
    64261 cttcttcttt tttcagctga attactttac catgtatttc atactttttt caatcggctt
    64321 ttttcaaaaa agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac
    64381 atattgagaa ataattaagg attacagcct ttactttatc atgaaataaa agggaaagat
    64441 aattttatgc caaagttttt atctattata cgtatacgca aaaaagctaa aacatttttt
    64501 gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt atttcttatt
    64561 tttcgaatga caggatgatt ttcccagcag gaggatgtcc ttcaactagc tgatgtgctt
    64621 ggatgacatg cttaagtgaa gcagacagat tcattgaaat aaaaacatga aaggctcctt
    64681 tttggtaggc tttgattatg ccagcaaagg cttctgcatc tagatattcc tttcttggaa
    64741 caaacgattc ataaaagcct tccttttttt gccgcaaatc tagatctggg aggtcattta
    64801 atgcaacata cctgccgcct ggtttcatga tctggatgcc ggtttctcct ttgatacttc
    64861 ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa ttgcagacct ggatttgttt
    64921 tatcataagc agcaaatgct gaagctccta atttttttac cttttcctca tttttactgg
    64981 atgcactggt taagattcgg attccctttt catggagaag ctggatcaga cttgacccga
    65041 ccgcacctga tgctcctagt atcattacag tatctgtcgg ctggatttcc gtcaaatggt
    65101 tgattagatt ataagccgta attcctggcg tgaccattcc ggctgcttct tcccaactca
    65161 ttttatctgg aatttttgct acttttgctg atggcaacac gacttcttct ccgtaagtcc
    65221 cgccgactgc atggacagct actcgatcac ctacatgaac agttgtgaca tctgaaccaa
    65281 tctctgtcac aataccagcg ccatcattac ctggtacata agggaatttc aatgttcgta
    65341 tctctttcat ttctcctaaa cgcaaggcaa catcataagg attcacactg aatgctttta
    65401 tttctacacg aacatgtcca gaaccgactt ctctgggatg agcatcgatt tcttcaaaaa
    65461 catcttgtgc ttctccgtaa ccgttaattg caaatcttcg catggtcacc atctcccttt
    65521 ttctttaagt ttacgtttat gcctttattt tgacaaaaaa aatgtctttt cgttttggcg
    65581 catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg tgttaaatta tttatctaaa
    65641 agcgtaaaga ttacgattta cgctaataaa aaataattgg aggaatttgt tgatggctaa
    65701 aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg attgaacgct atgctgcttt
    65761 acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg cgtaaacttc ctttagatgc
    65821 tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga cgccctcgcg gatacatgcg
    65881 taaattcggg atgtcccgta taaaattccg tgaattagca cataaaggtt tgattccagg
    65941 agtaaaaaaa gccagctggt aatccaagtc tggctgtaat gtaaaggagg agggcgcatg
    66001 gaccactcct tggatcatca aaaaagatac gttcgcttct tctagcagaa atgctggtac
    66061 tagaagcaga cgtatctttt tttacgcaca gctggtcctt ttttatatgt aaaggctgca
    66121 tggttacttc gctatattta tttagtaaca ggagtcagtg cttcgatacc cacactaatt
    66181 ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca aatggcagac cttttctttc
    66241 aatcttgatt tatcgatcgt tctgatctgc tccaacagga taacggagtc tctttctatt
    66301 cctgttccag tagctttgat cccaatgtga gtcggaagct taggtttagc catttttgcc
    66361 gttatggccg caacaatgat cgtcgggcta aaatggttac ctagattatt ttgaataacc
    66421 aaaactgggc gtgttccgcc ttgctccgat cccacgactg gagaaagatc agcaaaataa
    66481 atatctcctc tttttaccat aggatcctcc ttattcttga tattctcttg gtatccgctg
    66541 tcctaatccg caagccactt catagtggat cgtacctaat tgatctgcta tatcctgcat
    66601 agtgatttct ttgtctgcat ttttgccgat aagcgtcact tttgttccga caggaaattc
    66661 ttgaggcaat cggatcatca gttgatccat acatacacgc ccaatcgttt cacagtagtt
    66721 cccttctacc aatagagaaa aaccctgcat ctttcgtggc cagccatctg cataaccgat
    66781 cggtatcgtt ccgatccact ctgcttcagg agtgatatat gtttctccat agccgattcc
    66841 ttcacctgct gggagttttt tgacttgaat caattcagaa accagctcaa gtgctggctg
    66901 caatggataa acttccggta gtgcgtgccc agagggatta agtccgtaca ttgctactcc
    66961 ataacgaatc atattgccga ttgtttcatc atgccataaa gccgttgcac tattgctgac
    67021 atgtacatat cgaggaagtt cgggaagttt tttcagcact tctataaagc gttctttttg
    67081 cagattccaa tatgtatcat ctgcctgatc tgctgtagaa aaatgggtaa ataaaccttc
    67141 ccattctaat acacgacttt ctttgatgaa tgcagctgcc tcttttactt cttcaggtgt
    67201 acaaaatccg atacgaccca tcccagtgtc tgcttttaga tgaatcgaaa gaggcgtctc
    67261 tagcttactt ttctctaaaa cagcagctgc tgcttgcaac cattcccgtg tacctgcagt
    67321 tactgataaa tcatgagcaa cagctacagt cagtgcgtcg taaggaacca tattcaagat
    67381 aaggatgggc tgagtaaatc cagcttctct caactcgact ccttcatcta aatttgaaac
    67441 acaaaagccc gaagctccac ctgcaactgc tgcctcagct gtttcaatag ctccatgacc
    67501 gtatccgttc gcttttacta ctgcaaacaa ttcttttcct tgcggaagtc ttgcaacttc
    67561 atttgctaca ttttcagtta tagctttttt atgtatcact gctttcgttg ggcggtgcca
    67621 agcgacaacc atcgtttttc cttctttcta gtcttccaaa ataatttgtg caaaagctgt
    67681 ttcatttgta tgagtaatac tgacccatac attgccttca tgaggtgaac gcgtcacaac
    67741 aggctgacca ttttcatttt ttaaaatctc gacatcttga aaggtaactt ttccgatccc
    67801 agttccccaa gctttagaga aagcctcttt gcaagcgtaa cgcccgccta aaaattctac
    67861 ttggcgatga aaaggcaagg actggaatag tttcatttca tcagaggtca agattcgtgc
    67921 aatgaatttt ggtttttctt cgatcagccg agtgatccta ggaagttcga ctgcatcgat
    67981 cccaattcct tttatcatat gcttttctct tttcaataaa tttagaacac tctcattcta
    68041 ccaaattata accattttgc cacaaataag tccacaaata acaaaaatga aagtttctct
    68101 taaaaaagag aaactttcat tttatgttta atcgttattt gaacggatta caaatccgcg
    68161 ttttttatca ttgtgacgtt taccgccatc tttttggctg ttattatttt tgctataacc
    68221 gccttttgat ttgtttccac gataattacc gccgtcacga cgattacggt tgtttccgcc
    68281 accacgtttg ccatttttat tgtatccttt tttctgcatt ggcaatgggc gttctggtgt
    68341 gatcttcact ggcactgcat cagctggatc ttttgctgtc gtttttagca aaagtgcaac
    68401 taaatcttgt gcagaatatt cttctaataa tttatctgct gtttgcaggt atttatctag
    68461 accattttct gcaagttttg tttcgatttg ttcaactgca gcacctaatt gacctttgaa
    68521 tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt tttttcgtca agttttcaat
    68581 cacatgcaag tagtccattt catttggtgt aacgaaagtc acagacatac cgccttttcc
    68641 ggcacggcct gtacgtccga tacggtgaac gtagctttct ggatcttgag ggatatcata
    68701 gttgtacaca tgagtgacac ctgaaatatc taatccacgt gcagcaacgt ctgtcgctac
    68761 aaggatatct aaatgaccgc tcttaaatga acgcaatacg ctcattcgtt tttgttgaga
    68821 caaatcgcca tggattcctt ctgcacggta accgcgtgct tctaatccgc gtgccaattc
    68881 gtctacacgg cgttttgtac gtccgaagac gattgttaat tcaggtgttt gaacatcaaa
    68941 caagcgagtc atgatatcga atttttcgta ttcttttgcg cggacatagt attgatcgat
    69001 caagtcagct gtcatttctt tcgctttgat cttcacatga tgtggatttt tcatgaattt
    69061 aacgccaata ttcttgatag ctggcggcat cgttgctgag aaaagtaatg tttgtctttg
    69121 atcaggaact tgtgaaatga ttttttcgat atcttccaag aagcccatat tcaacatttc
    69181 atctgcttca tccaaaacta atgtttgaac ggttccaagt ttcaatgtgt ggcggttaat
    69241 gtggtcaagc atacgtcctg gtgtacccac aacgatatgt ggacgatctt tcaagccacg
    69301 gatctgacgg ccaatatctg ctccgccgta aacggcttgt acacgtactt ttttgtcgcg
    69361 ccctaaacga tataattctt cttgtgtttg gatagctaat tcacgtgttg gtgcgataac
    69421 taaaccttgt aattcgtgac gatctggatc gattttttct aacattggta atccaaatgc
    69481 agctgttttc cctgtacctg tctgtgcttg tccaataacg tcttttccag ctaatgctag
    69541 tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa ccagcgcgtt cgactgattt
    69601 caacaattct ggtgataatt ctagttcttt aaatttcaaa tgaatcctcc tatatggttg
    69661 ttaactgagg atgtagcttt tttgctaccc cgtcgatttg ttcggtcaaa gtgtattgtt
    69721 tctttttgac agtggccacg cacgttttta tccattctta gcccctgtgg caccaccgaa
    69781 aaatcacttt gcgaagttta ggcattagaa aagccaaacg ggtcgcgttc agctttatca
    69841 ttattatgac atctggcttc aacaagttgt ttctatggca aaaccagtcc aatttctctt
    69901 catctttcgg caataggtta ttctaacata gtttttcgtt ttctgcaagt ttacagattt
    69961 ttatttcatt tgctgaagtt gttctaccac ttcaaataag cccatgctgt tgctgccttt
    70021 tagcataata ctatctgttg cttcaagatt ttgtttgata gcatcaatca atttattctt
    70081 ttctttttca aaatagaaca caggtagttg ggggtatttt tggttcaatt cttcttttag
    70141 tgcatgcatt tgttccccat aaaggaaaat catggcgaat ttctcaggat cgatatgttc
    70201 actcatttgc gcatgcatat ttaaagactc tttccctaat tccagcatat ctgctaacac
    70261 agcgattctg cgtccttttg ttggcagatt tttaaaagta tctaatacca gtcccatggc
    70321 tgttggattt gcattgtaga catcgctcaa aatagctgca ccatttcctg ccgttagcca
    70381 ttcggttctg ttttgagtga tagaaacatg ggcgagccct tggcgtatct caggtatgga
    70441 cagtccaaag aagcgcccga tcgtatacgc aatcaaagca ttcgttacat tataacttcc
    70501 aggcagcggg atcgtaaatg tttctccttc tactacaaaa tcggtttgtg cttttccttt
    70561 tgctgtcact tccgcatgaa catcgccatt tccaataccg aatgttgtga ctgtttgtac
    70621 taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg ggcacaacga gtaatccgtc
    70681 tgatacaagt ccgtcagtga tttccatttt ggctttagcg attccttctc gcgacccaag
    70741 attttctatg tgtgcttcac cgatcattgt gattgctgca gcttttggtt gagccataag
    70801 tgaaagttcc gtgatttcat tggcgtgatc catgcccatt tccaaaacga tcttttctgt
    70861 atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata ttattgttgt aatttccttg
    70921 cgtcttatat gttttgtatt tttcagacaa gacagacgcg gttagatctt tcgtggtggt
    70981 tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct actttttcca agtaataggt
    71041 tgccaattgc tgcatggctt tcaaaggatc tgctacagga ataactggaa aattttttgg
    71101 agcttgttca acaggcaagc tccaaaatga agcagatgct ccgtttgtga tcgcttgctg
    71161 gataaattca tgtccatctc gcgtaccagc aagaggaacg aataaacttc cttcggttat
    71221 cttccggctg tcaaattcga cattggtaat ctttgtcagt gcagagcatt cgctgcccac
    71281 ggcttgtgca atttcttgta ttgttagatt cattgagatc tctcctaaaa aaggttgtga
    71341 aaaaagcgtt tggcctaaac ggcgggttcg catgactctc acttccaagt aaatttctct
    71401 attcgtattt agaagctaga atgaacgcgg ttacgcaatc actccagcct cttctttatt
    71461 tctctttatt catttttttc aaaaaaagat tgacgctgat ggtatcgatt cattcctaac
    71521 tggatcagtt cttcaatcaa atcaccgtat ttcaagccca tattttccca taagagtggg
    71581 tacatactga actccgtaaa tcctggcata gagtttaatt cattcaggaa taattcattt
    71641 ttatttgtca aaaagaaatc gcaccggctc aatccgcttc cacctaacat cgtgtaagct
    71701 aacttcgcgt actcttgcgc tttttgataa acttcttctg gcacttcggc tggaatctgc
    71761 atttcgattt tattattgat atattttgct tcataatcat agaatgctac gtcttttacg
    71821 acttcaccag gcaaagtcgt ccgaacgtct tcatttccta atacagcaac ttcgatttcg
    71881 cgcgcttcaa ttccttgttc aacgattgct cgagaatcat actgataggc tgttgctaaa
    71941 gcattttgca gctcttctcg gttttctgct tttgtaatgc cgacactaga acccatattc
    72001 gccggtttga caaacatcgg ataaagcaaa gaaccttcac attgatcaaa tactttttta
    72061 ggattttctt tccattgatt cttaagtact ggtacataag gaacttgcgg cacaccagca
    72121 gcttgtaaaa tatacttggt catgatttta tccatggcac atgcactggt caatacgcct
    72181 gcgccgacat aaggcatatt caatgtctct aagaagcctt ggatcgttcc atcttccccg
    72241 tttggcccat gtaaaactgg aaaaacgatg gctccttctt ctttgatttc gcccggattg
    72301 atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg ggtcccatga aagatgcaag
    72361 acatctttgc tggcaggttt ttctgttagt aatggacctt tgacccattg tccttctttt
    72421 gtaataaata cgagttgaac ttggtaataa ttataataaa tggcatttaa aactgaaaat
    72481 gcggaaagaa tggacacttc atgctctgcg ctgcgtccgc catatagtaa agtaatcttc
    72541 aaaagattgt cctcctgtat tctaaatcgt tcagtgattt actctcatat tttagcacaa
    72601 aacaaaaata aacgaataac ttatttataa aaaaacgagc cagcttctaa aagaacgagc
    72661 gtcatttatt ttttgtcagc tagattattt gtcccgtttt ttataaaaag ttatgataaa
    72721 gaaaaggagg gatgatgatg gcgcaaacaa atccacgcag ccgcttggca gaaatcattc
    72781 gagtgttcat caattacaat gtagtgccaa actttgtaca gcaaaaaaat cccgaacagg
    72841 taaaaaaagc ctttgaagaa ctaggcccca ccttcatcaa gataggacag atgctttctg
    72901 ttcgagaaga tttgctttct tctgccttca ctcaaacatt caaaacatta caagatagtg
    72961 tacctagcga cacattttca acggtcaaaa agacgatcga aacagaactt tctctttcat
    73021 tatcagatat attcgatgat ttttcaaaat cgccttttgc ctctgcttct atggggcagg
    73081 ctcatcgtgc caagctgaag aacggtgacg cggtagtcgt taagatccaa caccctaaca
    73141 tcgccgaaga gatccgactt gatcttcaac tatttgaacg agcgatccct ttgatcaaat
    73201 acattcctga aactagcgta gttgatctaa aaggggtact ccaagaagta aaacgttcct
    73261 tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg cgaacaattc tatcaaaaga
    73321 acaatggctg gaaagagatc cgcagcccca agatttatga cgctttttgt tcgaaaaaag
    73381 tgatcgtgat ggaagaaatg tctggtaaaa acttgaatca tttaatgaac gccgaaaaca
    73441 aaactgaaac ttttatcact ggaattcaaa acaagcagct gaaacaagaa gttgcaaagc
    73501 ttttagttga gaactttatg aaacaggtgt ttgatgatgg ttttttccat gctgatcctc
    73561 atccagggaa tctgctattc catgtactaa caaaagaaga acagacacaa gctagccgaa
    73621 aaactgaaac agtccatgaa aaagagttcg gttcctttgc ttttcgagcc tccacttctg
    73681 cagaagaccc tgttgcgcca tataccatca actacatcga ttttggtatg atggggcatc
    73741 tttctgcagg acttaggcaa aaactcacac aagccgtttt agcgctgtat acgaaagatg
    73801 cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca agaaggctcg tttgatgaaa
    73861 gtcggttcca ccaagaactg acaagttttc ttgaacagta ttatgattct cctattgatg
    73921 aaatcaactt gcaagaagta ttcacacatg tcgtgacgat ttgtcatcaa aataatctgc
    73981 agttcgatcg tgatatcacg cttttgctga aagcatttgg gacattagaa ggggtgatcc
    74041 gcgtcctcga tcctgaagtt tccctcatgg aagtcgcttc gccttttgct caacattatt
    74101 ttctcactca tctggatgta gaagatacat tgaaacaaag cggattagac ttattggaag
    74161 ggatgaaggc tgcgcctaag atccctcaac agctccacca tctattagaa atgtggactt
    74221 cgggacaagg aaaagtcaat ttggaattga aaaagcaaga caaattattg agcaggatcg
    74281 aaagtatgat caatcggctt gttttcggta tgattttagc tgcattgatc gtagggtcct
    74341 ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt cgtttcctta ttagggatat
    74401 ttacgtatgc tattgccgca ttcgtcatta tttttttggc aatcgatgca ttgatccaaa
    74461 tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga aagaatcagc ttcaagaaat
    74521 aagaaggggt tttacataaa ttgtttctca aatttttgtg aaaattcggc ttatttttcg
    74581 aagaggctac ttctgaaaca ccgagaataa aaaatgtgaa aaagctgttc tgcacccagt
    74641 aataagaaca atcaaaaagg gaacatggaa cacatttatc ctgtttccct gttccctttt
    74701 tgatgtattc gagcataaat aaaatacctt cgttacctac tatcttctgt acttggtaaa
    74761 ccttagtcaa ttgccttttt taacgcttga cgaaaagaca tatgatcttt ctccgtaaag
    74821 ggttttggac cctttgtcct tatgccagct tttcttaact ttccactatg ataacgttgt
    74881 tctagaagcg tgccgatatt cgcttcgtca aattcttttc cagagtgatg gaacactcgc
    74941 acacccttcg gcaatacaag tgaagctaaa ccataatctt gcgtgatcaa aaagtcattc
    75001 ttcttaacca taccgacgat tttataatcc gcactatcag ctccgcggtc tacgtagacg
    75061 atttggacat gattagcgta ttctttggtc gtataatggt ccacactggt cacgataacc
    75121 acgtcaagac cataggattc tcctaatttt atgacatctt cttttactgg agaaccatct
    75181 ccatcaatga atagcctcat tctttttcgc cttcttccat aaatgataaa cagttactaa
    75241 tccgctcagt aaaagaccaa cattgatcca ttttgccaag ataagcagtt gtacactttc
    75301 tatcggcact gattcatggt tcagcgtgat caataataga tggattggct ctcccatttc
    75361 ttttgctaaa acaaacaaaa gcgtcatcaa taaaaactgg tggatcacac tccctttatt
    75421 ttgagtagta tacttgacaa cgatccggcg atccattgga atagctgtat cagtttgata
    75481 atattcctta tttagccagc tgcctgttac tacgtgccag aaactaagca gaaagacacc
    75541 tatcgcaaaa ccatttggaa tcaagaggac caataaagaa gaactcagtc caagcaattg
    75601 catagccaat ggagaccatg ttaaacgttt caaaatccat ggtccgacta tttttgctgc
    75661 aatcaagcct gctgcataag gcaaatacat aaaaatcgaa agtttttcta atccgtacag
    75721 tatgccaaca taaaaactgc taaataaaaa aaggaaattc cctagcattc catttagaaa
    75781 agtaaacaga tttagccagc ttggcaactt ccattccttc ttctggtgtc ctaaaacaaa
    75841 cacgaacaag atgaacagca gcaagaaacc ataaatagca agatcaaaaa gcctttcatc
    75901 gaataacaat cgggcatttc tcaaaagaaa caataagcca aagaatatca aaaataaaac
    75961 aagctctcgc gtagctacca gatttctttt gatgtctttg aaatccagtt cgtaacgcgg
    76021 ataatgacta accgtatggt aggccatgac gtaaaaaagt gtataaatag taaagcttgc
    76081 tgcaatctga gtcggtgcag gcaactcaat cgcttgatac aggagaaata gccagatagc
    76141 tgcaaataag tactttttcc catccatatt gataaaacct tgcgtctttt cgtgaaaatt
    76201 gaccgtggta ttagcaggta caagccatgc tgcactcagc cctagcaaaa cacttgaaaa
    76261 ataaaataaa ggaaaataaa atacgcctaa tattccaagc aatgaaccag caccaccaat
    76321 cagcagagcg atcatcagca acgtgtaact atctagacca aatcgtagac tgttgactaa
    76381 aaatagtcca gtcatccgaa aggcataaaa cagcgtaaat ggcaaaatcg tcagccaatt
    76441 atgcgagtct gttagtccca aaaataaaag ataaggaata aaaataaccg tgttacttaa
    76501 gataaagggc gttgtttgtg taaaattacg cccaaatcgt ttgagttttt cgaacatgac
    76561 aatggctcct taaacagtct tataactaat ctgctcttcg attttagcat taaaggggaa
    76621 ataaaccaat tttacagaat atattcttgt aaaaaaaaat acagaaagaa gcataagctt
    76681 ctttctgcat ttttacggta cttgattact caagcagaca agcccttctc cacaaaagtc
    76741 ttgcccgaca cccctgcatc cggcaggtga taaagataga aatcaattat cctgtgcctc
    76801 caaaatcaat taggagctct gtaaaccaga gtccttctcg acccgtcgca taaatttact
    76861 ttaacacagt tcctatgttt atgcgagagc ttttttcatt ttataatcga ccatttgaaa
    76921 tccttgaccg atatctgaaa taagttcttc tgtaatctca aatccttcat gcttataaac
    76981 ctcgattgct tgatgattcc cctgattcac tcgcaaaaag atctctcttt cctttgtttg
    77041 cgcagcaagt tcttcatacc agcgaaaaac tgaagaagtc aatccttgac cacgtacttt
    77101 atttagcaaa tataatttac ttaaataaat ttttccattt atctcttcat aagcagtgta
    77161 accgacagtc ttttcttcgc tttttaataa aaagtaggac acaccttctg tcagttcttt
    77221 ttgaatctgt ttttttgact gataagtttt cagcatgtaa tccacttgtt tttgaccaat
    77281 gatcggcaca aaaacttccg gccatacttc ttgtatgact gccatcagct catccagttc
    77341 attttctgtt acttttattt tttccataca cccacgtcct tttttacatt ataaatgacc
    77401 ttgcgcttga tggaataaaa agagcatgat catgctatac acacccaccg accacggttt
    77461 caagaaaatc acaatctgca taaattccct aaaagataat ttcgtcaaac cagctactag
    77521 acagatcaag tcgtctggcg caaagggaag cagcaaggta acaactagta cttttttaat
    77581 atctttcgtt tttcttgtca gcaattcatc aaatttctga tacttttttg gcgataaaat
    77641 aagctggaca aaggctacgc catagtaacg gacaagcaaa aatccgattg cttccccaat
    77701 cactaggcca atacaactat ataaaagccc aaatccattg ccaaacatca gcatgcccgc
    77761 tactgaggag atccctcccg gcaaaatagg aatgatcact tggatcactt gcaacagaac
    77821 aaatatcatc actgcatact tgccaaattg ctggataaac gcttgcaaag acgtgctcga
    77881 tcgaaaaatc ccatgatgat aagcataaag aatcaacaca aagaaaaaaa acagaccaat
    77941 aatgggcatg atctgaagac atttttgtaa aactgatcct tttttagaca tctatattcc
    78001 ccacatcttt tttcatttat taaaactgcc tccttatgaa ctgaacccca aaagttgaac
    78061 tatttaatgg actgtttccg atattctact ggagatagtc catttaactt tagttttatc
    78121 cttttgttat tataccacct aatatactca tttaatttac tttgaaatat ctcaattgat
    78181 cggaattttt ctcgataaaa aaactctgat ttaagcacac caaaaaaatt ttctattaca
    78241 gaattatcta agcaatttcc ttttctagac atgctttgaa taatgttatt ctcttttaat
    78301 tttttttgat attgtggcat ctgatactgc catccttgat ctgaatgtag aatcagtgaa
    78361 ccctcagttc cctttttttt aattgcttgt tgaagcattt cttcaatcag tttatatgtt
    78421 ggactagttg atatactata actaattatt tctccgttaa ataaatcaag tataggagat
    78481 agatagattt ttcttccttt tatcttgaat tcagtgacgt ctgtcaccca ttttttattg
    78541 ggtgtatcta ctgaaaaatt tcgttttaac acattcttgg caatttttcc tactgttcct
    78601 ttataagatt tatatcgctt tattcggatt tgacaggtaa gtcccatttg ggacattaat
    78661 tttctaactg ttttatgatt gattgtatat cctttcatct ttaacgctaa agtgactcta
    78721 cgataaccat aagagtttct tgattctttt acaatcgctg taatttcttg ctttatcttg
    78781 ctatatttat ctggcttatc taattttttt acccagtaat aataagttga cttcgctaat
    78841 tgtgcaattg aaagtaatag attcaattta aattcttttt tgagctgaag gattgtttta
    78901 accttgattt cttcttgctc aaatcttgtt cttgaatcaa ggtttct
//
LOCUS       DAYYBH010000005        76959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000005 DAYYBH010000000
VERSION     DAYYBH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.005"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..331)
                     /locus_tag="ACHAUG_000373"
     CDS             complement(<1..331)
                     /locus_tag="ACHAUG_000373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479495.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(345..602)
                     /locus_tag="ACHAUG_000374"
     CDS             complement(345..602)
                     /locus_tag="ACHAUG_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479496.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(797..1339)
                     /locus_tag="ACHAUG_000375"
     CDS             complement(797..1339)
                     /locus_tag="ACHAUG_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8479497.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1360..2451)
                     /locus_tag="ACHAUG_000376"
     CDS             complement(1360..2451)
                     /locus_tag="ACHAUG_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8479498.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2459..4669)
                     /locus_tag="ACHAUG_000377"
     CDS             complement(2459..4669)
                     /locus_tag="ACHAUG_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8479499.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4685..7213)
                     /locus_tag="ACHAUG_000378"
     CDS             complement(4685..7213)
                     /locus_tag="ACHAUG_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8479500.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7265..7675)
                     /locus_tag="ACHAUG_000379"
     CDS             complement(7265..7675)
                     /locus_tag="ACHAUG_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8479501.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7688..7906)
                     /locus_tag="ACHAUG_000380"
     CDS             complement(7688..7906)
                     /locus_tag="ACHAUG_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8479502.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7903..8895)
                     /locus_tag="ACHAUG_000381"
     CDS             complement(7903..8895)
                     /locus_tag="ACHAUG_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8479503.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8974..9885)
                     /locus_tag="ACHAUG_000382"
     CDS             complement(8974..9885)
                     /locus_tag="ACHAUG_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8479504.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9904..10248)
                     /locus_tag="ACHAUG_000383"
     CDS             complement(9904..10248)
                     /locus_tag="ACHAUG_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479505.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10268..10546)
                     /locus_tag="ACHAUG_000384"
     CDS             complement(10268..10546)
                     /locus_tag="ACHAUG_000384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479506.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10905..11405)
                     /locus_tag="ACHAUG_000385"
     CDS             complement(10905..11405)
                     /locus_tag="ACHAUG_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479507.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11406..11951)
                     /locus_tag="ACHAUG_000386"
     CDS             complement(11406..11951)
                     /locus_tag="ACHAUG_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479508.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11948..13204)
                     /locus_tag="ACHAUG_000387"
     CDS             complement(11948..13204)
                     /locus_tag="ACHAUG_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8479509.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13565..13876)
                     /locus_tag="ACHAUG_000388"
     CDS             complement(13565..13876)
                     /locus_tag="ACHAUG_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479510.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13899..15374)
                     /locus_tag="ACHAUG_000389"
     CDS             complement(13899..15374)
                     /locus_tag="ACHAUG_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8479511.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15823..16275)
                     /locus_tag="ACHAUG_000390"
     CDS             complement(15823..16275)
                     /locus_tag="ACHAUG_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479512.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16277..16642)
                     /locus_tag="ACHAUG_000391"
     CDS             complement(16277..16642)
                     /locus_tag="ACHAUG_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479513.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16741..21024)
                     /locus_tag="ACHAUG_000392"
     CDS             complement(16741..21024)
                     /locus_tag="ACHAUG_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8479514.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21171..21410)
                     /locus_tag="ACHAUG_000393"
     CDS             complement(21171..21410)
                     /locus_tag="ACHAUG_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479515.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21500..21742)
                     /locus_tag="ACHAUG_000394"
                     /pseudo
     CDS             complement(21500..21742)
                     /locus_tag="ACHAUG_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21855..22106)
                     /locus_tag="ACHAUG_000395"
     CDS             complement(21855..22106)
                     /locus_tag="ACHAUG_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479516.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22269..23228
                     /locus_tag="ACHAUG_000396"
     CDS             22269..23228
                     /locus_tag="ACHAUG_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8479517.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            23398..24078
                     /locus_tag="ACHAUG_000397"
     CDS             23398..24078
                     /locus_tag="ACHAUG_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8479518.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            24168..25202
                     /locus_tag="ACHAUG_000398"
     CDS             24168..25202
                     /locus_tag="ACHAUG_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8479519.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            25216..25551
                     /locus_tag="ACHAUG_000399"
     CDS             25216..25551
                     /locus_tag="ACHAUG_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479520.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            25535..28015
                     /locus_tag="ACHAUG_000400"
     CDS             25535..28015
                     /locus_tag="ACHAUG_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479521.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            28071..29630
                     /locus_tag="ACHAUG_000401"
     CDS             28071..29630
                     /locus_tag="ACHAUG_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8479522.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            29627..30991
                     /locus_tag="ACHAUG_000402"
     CDS             29627..30991
                     /locus_tag="ACHAUG_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8479523.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            31162..32232
                     /locus_tag="ACHAUG_000403"
     CDS             31162..32232
                     /locus_tag="ACHAUG_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8479524.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            32247..32915
                     /locus_tag="ACHAUG_000404"
     CDS             32247..32915
                     /locus_tag="ACHAUG_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8479525.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            32935..33513
                     /locus_tag="ACHAUG_000405"
     CDS             32935..33513
                     /locus_tag="ACHAUG_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8479526.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            33914..34825
                     /locus_tag="ACHAUG_000406"
     CDS             33914..34825
                     /locus_tag="ACHAUG_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8479527.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(35203..36120)
                     /gene="rnhC"
                     /locus_tag="ACHAUG_000407"
     CDS             complement(35203..36120)
                     /gene="rnhC"
                     /locus_tag="ACHAUG_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8479528.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            36322..36759
                     /gene="zapA"
                     /locus_tag="ACHAUG_000408"
     CDS             36322..36759
                     /gene="zapA"
                     /locus_tag="ACHAUG_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8479529.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            36817..37365
                     /locus_tag="ACHAUG_000409"
     CDS             36817..37365
                     /locus_tag="ACHAUG_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8479530.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            37480..39840
                     /locus_tag="ACHAUG_000410"
     CDS             37480..39840
                     /locus_tag="ACHAUG_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8479531.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            39927..40241
                     /gene="trxA"
                     /locus_tag="ACHAUG_000411"
     CDS             39927..40241
                     /gene="trxA"
                     /locus_tag="ACHAUG_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8479532.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            40423..42201
                     /gene="uvrC"
                     /locus_tag="ACHAUG_000412"
     CDS             40423..42201
                     /gene="uvrC"
                     /locus_tag="ACHAUG_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8479533.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            42346..43245
                     /locus_tag="ACHAUG_000413"
     CDS             42346..43245
                     /locus_tag="ACHAUG_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8479534.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            43238..44500
                     /locus_tag="ACHAUG_000414"
     CDS             43238..44500
                     /locus_tag="ACHAUG_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8479535.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            44661..46559
                     /locus_tag="ACHAUG_000415"
     CDS             44661..46559
                     /locus_tag="ACHAUG_000415"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8479536.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(46889..47608)
                     /locus_tag="ACHAUG_000416"
     CDS             complement(46889..47608)
                     /locus_tag="ACHAUG_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8479537.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    47798..47845
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            47945..48277
                     /locus_tag="ACHAUG_000417"
     CDS             47945..48277
                     /locus_tag="ACHAUG_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479538.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            48264..50267
                     /locus_tag="ACHAUG_000418"
     CDS             48264..50267
                     /locus_tag="ACHAUG_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8479539.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            50286..50756
                     /locus_tag="ACHAUG_000419"
     CDS             50286..50756
                     /locus_tag="ACHAUG_000419"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8479540.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            50777..51361
                     /locus_tag="ACHAUG_000420"
     CDS             50777..51361
                     /locus_tag="ACHAUG_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8479541.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            51376..52362
                     /locus_tag="ACHAUG_000421"
     CDS             51376..52362
                     /locus_tag="ACHAUG_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8479542.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            52352..52663
                     /locus_tag="ACHAUG_000422"
     CDS             52352..52663
                     /locus_tag="ACHAUG_000422"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8479543.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            52685..54466
                     /locus_tag="ACHAUG_000423"
     CDS             52685..54466
                     /locus_tag="ACHAUG_000423"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8479544.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            54459..55835
                     /locus_tag="ACHAUG_000424"
     CDS             54459..55835
                     /locus_tag="ACHAUG_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8479545.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            55842..56477
                     /locus_tag="ACHAUG_000425"
     CDS             55842..56477
                     /locus_tag="ACHAUG_000425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8479546.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            56507..57862
                     /locus_tag="ACHAUG_000426"
     CDS             56507..57862
                     /locus_tag="ACHAUG_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8479547.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(58054..59448)
                     /locus_tag="ACHAUG_000427"
     CDS             complement(58054..59448)
                     /locus_tag="ACHAUG_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8479548.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            59540..60511
                     /locus_tag="ACHAUG_000428"
     CDS             59540..60511
                     /locus_tag="ACHAUG_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8479549.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            60508..61290
                     /locus_tag="ACHAUG_000429"
     CDS             60508..61290
                     /locus_tag="ACHAUG_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8479550.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(61459..61830)
                     /locus_tag="ACHAUG_000430"
     CDS             complement(61459..61830)
                     /locus_tag="ACHAUG_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8479551.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            61964..62407
                     /locus_tag="ACHAUG_000431"
     CDS             61964..62407
                     /locus_tag="ACHAUG_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8479552.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(62450..63259)
                     /gene="yidA"
                     /locus_tag="ACHAUG_000432"
     CDS             complement(62450..63259)
                     /gene="yidA"
                     /locus_tag="ACHAUG_000432"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8479553.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(63278..64645)
                     /locus_tag="ACHAUG_000433"
     CDS             complement(63278..64645)
                     /locus_tag="ACHAUG_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8479554.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(64629..65459)
                     /locus_tag="ACHAUG_000434"
     CDS             complement(64629..65459)
                     /locus_tag="ACHAUG_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8479555.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            65622..66059
                     /locus_tag="ACHAUG_000435"
     CDS             65622..66059
                     /locus_tag="ACHAUG_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8479556.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            66135..66755
                     /gene="rpoE"
                     /locus_tag="ACHAUG_000436"
     CDS             66135..66755
                     /gene="rpoE"
                     /locus_tag="ACHAUG_000436"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8479557.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            66944..67450
                     /locus_tag="ACHAUG_000437"
     CDS             66944..67450
                     /locus_tag="ACHAUG_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8479558.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            67467..68366
                     /locus_tag="ACHAUG_000438"
     CDS             67467..68366
                     /locus_tag="ACHAUG_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8479559.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            68453..69337
                     /locus_tag="ACHAUG_000439"
     CDS             68453..69337
                     /locus_tag="ACHAUG_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8479560.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(69529..69600)
                     /locus_tag="ACHAUG_000440"
     tRNA            complement(69529..69600)
                     /locus_tag="ACHAUG_000440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69565..69567),aa:Arg,seq:ccg)
     gene            69660..70994
                     /gene="rpoN"
                     /locus_tag="ACHAUG_000441"
     CDS             69660..70994
                     /gene="rpoN"
                     /locus_tag="ACHAUG_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8479561.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            71143..72234
                     /locus_tag="ACHAUG_000442"
     CDS             71143..72234
                     /locus_tag="ACHAUG_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8479562.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            72275..73276
                     /gene="gap"
                     /locus_tag="ACHAUG_000443"
     CDS             72275..73276
                     /gene="gap"
                     /locus_tag="ACHAUG_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8479563.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            73386..74576
                     /gene="pgk"
                     /locus_tag="ACHAUG_000444"
     CDS             73386..74576
                     /gene="pgk"
                     /locus_tag="ACHAUG_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8479564.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            74680..75435
                     /gene="tpiA"
                     /locus_tag="ACHAUG_000445"
     CDS             74680..75435
                     /gene="tpiA"
                     /locus_tag="ACHAUG_000445"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8479565.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            75551..76849
                     /gene="eno"
                     /locus_tag="ACHAUG_000446"
     CDS             75551..76849
                     /gene="eno"
                     /locus_tag="ACHAUG_000446"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8479566.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        24693 a        14351 c        14858 g        23057 t
ORIGIN      
        1 atgaattcat tccccaaagg ccttttaaga ttgatacagc agtccgttca cttcttttag
       61 acttgtcata caagtctttt aaatgatccc atatcagact agtttgtcta tctagcttca
      121 atccattaaa cgctaatgaa cggttcattt tttctattac tttagataaa gctacagaaa
      181 tttttatacc ttcacaacgc gcatcttgga tttcaataat agtatcatca attagttttt
      241 gaatttcttg cctatcaaca tattcaatct tagaaagaga atcttttaaa gctactaatg
      301 attcaacgat ttcttttttc tcttcagaca ttttcttcct ccttctagtg acccgtaaag
      361 ccatgtagag cagagtccat tgaaccagta attacactat ctacaacgcc attccaattt
      421 actctacatt ttccattttt cagatttgtc ggtccatacg gatagccttt ttgacattca
      481 atgttcggat tataccagat aggattatac cattcagccg cagaagcaga ttcagtcgat
      541 acaaacaatc ccacaccaac aaatccaata cacatcacac tatagccaat tagttttttc
      601 atctcgtatc catctcctaa aaaatagaat agttcttata gttattcata agacatattt
      661 tttccgtttt tgttttatcc tttctgtcct ataacaactt ttttcaagta aagaatgaca
      721 tagtttttct taaaaaaaca gtctgttttt ttctatttct tcggtgtata ataaatatga
      781 gtgttcattt tttattttaa tacgcactcc gtacataata aatcttgatc gcatcaatct
      841 tttgagcttc ttgatctact ttcaattgaa gtaatacaaa ttgagtaacc ttattcattg
      901 tttcttctcc aaataacaaa tattgatccg gattctctat atcttgtgtc acctggtaaa
      961 gtttcccagt tgttttcatt tgtacatgcg ggtctacatc aatactattg tctttaatac
     1021 aacgatctgt catatagtcc ctcacagctt gattgcgttt ataaacatct gtgaaattac
     1081 tccattttct agcaaaatct tttaagaaat tttttgtcat ttcatctatt tgcatttgtt
     1141 gcgctgtctc gtcttctaat gaaccaattt gactgttact ttcagtactt tgaagagttc
     1201 cacttgtttc ttttactgct tcgattgttt cttctgtcgt tctcaattgt gttttttgtt
     1261 tttcagaaga aaaccaatga caaccagtaa gacctatcaa acataaccaa ctaaatacta
     1321 aaattatccc ttttttcacg tttctagtca ctgcctttct tatttcacct tacgaaccag
     1381 actacgaatt tttgtttgat tgtacgtgcg ttgataccga gccataatcc gtccttgctc
     1441 ggcattctgt tccacactag agaaatgttg tccgccatat gaaacatctg ttataattcc
     1501 ggtgtgacca tagattccag gagacaatgc accacctgct tgccaattca ccacatctcc
     1561 tgcttgtaaa tcacttggtt ttgggttcat gattacttgc caaccatagg ttgtccagtt
     1621 ataatcttcc cctattaaca tcgcaaacat gtgtccagaa cccattaact gtgggccacc
     1681 taattgagat acataccatg ccgtaccacc ataacattcc ccattattta cggatcgtcc
     1741 aatgatccga tccaaaacct tcgcattgcc tttacttttt cctgcaataa gcgaagtttt
     1801 ttttgttggt aggctattcc aatgcttgat cgcaaaagta tcatattccg ttaagttata
     1861 ggacgttatg atactcattg ctcctcgata atacggactc ccttcggtaa tacctgctgc
     1921 cgcatagcct ccacgaccta gaatcaataa acaacttcca ggatctttat tgcccacaca
     1981 ttgattaaag ttgctttggc tcgccaccat aaagccataa tagtcataac aagcttttaa
     2041 gttcggaaac ttcatataga aaccaccttc agaaccacca attccacgtg cagaaccttg
     2101 tgggtaggga caaccaggaa accaggtaat cccaaaaaag ttcgtatcgt ttcttccaga
     2161 ttgtgactgt ccataagctg attcatagcc taattgagaa agaatcacac tcggtaataa
     2221 ctgatacttt acagctgcac caactgctgc ttgcgcatgt tcatctgtga ttggaccacg
     2281 tcctttaatc tctgaaaaaa tcggtaatcc ttcggtatat tccccattaa aagcgacagc
     2341 tagacctgta gcttctcctc catgctcaga aagttctcct gaaaaaaaga atggcaatag
     2401 aaaaaaggga agaataaaga aaagaagaca acctagacac cctttcttca tttcttcctc
     2461 atcctttctt cgataatggt ttttggactg ttcgtccttt tggtaaagta aaatcagttc
     2521 ctggttttgc gtccaaaatc ggcagtgttt taggatcctt ttctattttt tccgattgct
     2581 ctgttggctt atctaaagaa gaaaatgcag gttgtaacga ttttggtgcg gaaatcgacg
     2641 gtttactttc agacgaacgt tgccccggtt gttgcacaga acgatttcct gatagagtgg
     2701 ctaccgacga attaggaaca gagtattggt tcaataactg ctgcattgct ggataactcc
     2761 catttttcgc ctgatttttc acttggcgaa tctgttgtga aagagaacca gcagctgtta
     2821 attgtggcac gggtactttt ccagaaggca acggaatcga ttgaccaaca aaagcgatac
     2881 ctttatttaa caatggtttt ctttgattat tcccttgttg cttactcaat tgtgccaatg
     2941 attgtacctg ttgttttggt cttactgaat tcatggacga atttggaatc aaaccatttg
     3001 aaagaccaga attccactct gttttcgatt tggtagaaga catttcaggc tctaccccat
     3061 gataacgaac atgacgacca ctataaggca aggcttgatt tgaacgccca cctagttcaa
     3121 agggattaac cacttgcctc ccttttaccg attcttgtgg tgtacgtttt tgttgaaact
     3181 tttgaaatcc atgtatcgct gcagaagcga gtggattcgt tgctcgatac acattccctg
     3241 ctaatcctgc acctttgatt gccaaatttt gtccagcagt tgatagttga ccaggcagtt
     3301 gtttcatttg agacaaagaa tgacttgcta tgtttttcgt gttgtttata gtttgatctg
     3361 cagctcctaa ccctagtttc tttaataaat ctttccgttt tacaatgagt aaaattgata
     3421 ggagtgcttt gacaaacata tttcctaaaa aaccaaaaac agtattcatt ggaattaaat
     3481 cgtctactat gttatagatc aaaaatgcca ccaaaataat cacactgtaa aaagattttt
     3541 gaaacatcaa gcccaaaaag gactttaagg tattcatgag catataatca aatactggaa
     3601 caaaagaagc gagtaatgaa aaaggaatca caagtaataa acacaacacc aacatttgaa
     3661 agataaaacg aatcatgcca atcactacaa gtggtaatcc aatacttaga ttaagcaatg
     3721 gcaccataga accaacgact gctttataaa cagctttagt tggttgaata tagacacgat
     3781 aagccttctt tttccctcta tcttctcctg cagcagtggt cacttttgtc tcaatatcag
     3841 ataattcttc acttgacgct tccttggcta gaaattcttg tggttcaatc tctgcttgat
     3901 acaattcttc taaatttgtt gtcccataat ttaacaatat atacggatca acaacagccg
     3961 ctttataata aagttggcgc actttttgaa tggcttcctt ttgtgtcgtt ggcactaaaa
     4021 caccattgac atcttttgtc gccaattcat ctagactatt tgtagcagaa aaaataaagc
     4081 cttctacctc cacggacaaa tcatttaacc ctttggtaaa agcttctcct ttactaggta
     4141 cgttactacc atagccaaac caaaccattg aaaaaatgac aacgaagaga aatcgaataa
     4201 acaccatttt tgcgtaatgt ttccctctaa atatacgaag agtaaataga tataacacat
     4261 acaagacgac aatccccgca ccaataggat cgtatatttt tgaataaata gtatccgaaa
     4321 aagtgaaaaa ctggtgaatt aactcgttca ttgcagctcc ttcatagagc ttctcaatga
     4381 caaaatcaag gccttgccaa atcaatttac ttaaactaaa gaacatattc gtcatcattt
     4441 ttagaaaatc tgtgccattt gccccaaata acattttgcc taatgaaaat tcactatcag
     4501 ataaataaga agaaaaggta tcaatatgaa atttcccaat ctcatagatc gattgatcca
     4561 cctgcttttc tgccgcatac accaatccac caacaaaaag cagtccgaat ccaatcgtta
     4621 aaaaaaacaa aacgcaccat ttcttttcct tatgctttat ataagccatt gtttcacctc
     4681 atttctattg catgattaac gtatcctgtt cttctacttg agagtgactt gttttttccg
     4741 ttgtttgtaa tgcagcggtc atttcttcaa acaaactgtg tacattgagt tttcctacac
     4801 gtccataggg atctaacatc caacactgtc ctttaatcat gtggctcatt tcagctaagt
     4861 tcgcttcatt tacttccaaa cgtaaatgat tcaaaatctt ttctcgctca ctgtcttcat
     4921 caaaagcaaa gacgacacca aaattccctt tttctttatc tgaacctaag tcaccaataa
     4981 attgtgtatc taacactaat tgattcatct gggaacgccc cacacgtgcc attgctgcaa
     5041 taatactacg tcctgcgttc gaattattaa atacccatgc ttctgtaaaa taaatcgctg
     5101 ttttcttact actatctcgt tttccaaaca tttcacaaaa acgacctaaa ctaatcatca
     5161 aacacaaaga ctttcgatcc gcatctgtat atagttctgg tcggacattg tcatttggta
     5221 atttcaaatc ttggatctct aaaatcgttg ttttggcatt aaagtctaat cccgggtttt
     5281 ctccataaga aaatcctaaa cgtaaaatcg aatcttctac cgtcaagcgt aaaaactcac
     5341 catattctcg aacctctttt tgttccattt gttctaaata gtcgaccaca tgcatcaagc
     5401 caacttgttg tccggcttct ttttgaacta aaacttcttg gattcccttt aataccgccc
     5461 cttttaaaag gtgattggta cctaacggac ttaactcatc aatcatatcc tgggctactt
     5521 gtttcgcatc tactcgggat aaacaaacaa ttggatctaa tacaccataa ttacggggat
     5581 ttgtagcatc taacgtgaca taactcattt tttgcaaatg ttctacaaac aaagggtaat
     5641 gtttctgata atacgggtct tgaatgagtt tcgtaaacca tttgcgcttt tctcctttgg
     5701 gatccacaaa taaactttgt acattcataa acgaactata caaaaagagc aagccaacta
     5761 aaaaggtctt ccctttaccg gtttttccag ttactgctac atgaggcgca tcaaacaacg
     5821 cacctttgat tccttccgca atggctagaa gattcaaaaa gacaaaacga tttgacgcat
     5881 aaattaattc ttctaatggt gttttttctc ctcgagaacc accatcccct aatgtatctt
     5941 gccgaccaat ataccagccg gattccatac ctaattctga tactgctgaa aacaaccatt
     6001 cgccaactgc ttctacagtt gttcggtgct tccaatgtaa ctgccgtccc aatggttgcc
     6061 ctaataaaca tttatagaac aattctcgtt gatcattttt tgcacggtgg atatggataa
     6121 tcgtatgaag ttgttgaata accgttgcac tattttgacg aacttcttca accgtttgac
     6181 cgtatacaat aaaacatgcg atccaatcga cgacaggttg cccattatct actcgttgct
     6241 ttaaatgttt tactaatcct cgaacagtta ataagcgttc actaggttct tcatcatttt
     6301 gaaccaattc cttatgctct gttttgaacc gttccgatag ccaattcagt tttaaaccaa
     6361 tacctccctg tcctttcaag cgtggatagt gaattttcat tcgcaattct gctgggaaag
     6421 aaagatcctg agcaaattga aatagatcac aatttctcat attttcaggt gtttccccaa
     6481 taggcaacaa tgctaaataa ctactcccta actcatcttc taattgaaga aggcctttat
     6541 gttccttcgg tatacgtaca tacgttcctt gattgtcttt cagtacttcc caattatttt
     6601 gttcttctac tttgtgagcc attcctcgaa tatattgcag acgattgaga tacaacagct
     6661 ctttttttac taagggacga atagaaaagc tataaagtaa ttgtgtcact tcttttacat
     6721 aaggatcact ttcagaaaac aaagtttcca aatcatctac ttctaatcca aataagcgtg
     6781 ctaaacttcc tgctaaatct aaccagatta gatttattac atcatgaaaa ctctccaccg
     6841 ttgctttttc tttgactttg attcctaaaa taaatgaagg acgatagact tgaccttctc
     6901 gttccaacaa tgtcactgtt tcttttgcat aatacgcacc aattgcttga ctatcgggag
     6961 aaaatccctt tcctaatgtg tcaaacttcg tccgtaaatt cattgttcgt ggatacatca
     7021 ccaactccac atcttcataa tctgctaaaa gcaacagtaa acgagccatc atttctttta
     7081 tttcctgtgt cttttttggt gtgttttttg aaattgttcg tggaaaaaca tgatagtagg
     7141 cccagataca atcctctcca tttaacaaac aatttttctt gaatgctttt actggttctt
     7201 ttacaagcac caatgaattt cacctcccaa taaaaaagga acaaccagtt gattgtccct
     7261 ttttctaatc ctctaaataa acatacttat tcacaaaaag taattcttcc aatgcattgt
     7321 ttgatacctt gtcccagtta taatacacac aatcaccatt catcatacga atcatataac
     7381 aaaaccaatc ccataaataa aaatgcactt tttttccatc aggttttagt tttgccagtg
     7441 ctaacacaca taaatagggg agtcctatat aaaaaacaac tcctgctggt ggaaaaaaag
     7501 aaatgaccca accaataaat gaacgtaaaa ataaatattc aaagattacg accaacaaaa
     7561 aagttgctgc attgactaat gcaactggaa atggaagacg ccaattgctt gtaatctgtt
     7621 gtacgcgata aggttccgca taaacaatcg aataatcata aggttcccaa tccacttcac
     7681 tatcctccta atccataaat aatttttcaa tgattttacc aaacgcatca aatacacgtg
     7741 ttggatcatt taagaagaaa taaactgcgg cacctgctgc gatagatatt aatgcatttc
     7801 ctacttttga tttcataaaa tttcgcaatc ccagataaac aaccaccatt attaaaaaat
     7861 agccaccttg cgttaatata aaatctacaa ttcccttcag tgtcatttcc gttcttctcc
     7921 tttcctatcc aaccaattat gtgttaattt ttcaacataa tactgatttg tttttttcgt
     7981 aatgacaatc gtcatttgtt ctttttgtaa aagtgacgta tcaccatctc gaaagacgac
     8041 cgttgctttt acaataaaag gttttttcgt ttctttataa cgcaatactt tcatctctac
     8101 ttgatcaact ttagctgttt gattgagtcc ttcaggttct ttcatcatgt atgacatatc
     8161 ttttgcattg tttttcgcat acttatctaa gaaatcttcc acaaatcctt caacatcttt
     8221 cgcttccgac gcatcaaccg tttcgtataa tgtgtcatca taagtaatca ttcgttgttt
     8281 ttctgtattc tttggtagtg ccgtaaaata aggataagca ataatacgac agcccttgtt
     8341 tgaaatagaa aagggaagat tgagtacttg tgtgattgtc acacttgttt ctttcttact
     8401 ctctggattc atcgagcgaa tatcataagt tactttataa ctagcagttg gaatggtttc
     8461 aatttctttt aatccaataa acgaagcagc agttaatttt cgaacacctg aaacacttcc
     8521 tggttcttga ataaagtttt gtgcataaaa ctcttttttt aatgtttctt gacgttgttg
     8581 ttcttgttca tgattatcga atgtattcat atacaaagca acaaaacgat tcataaagga
     8641 ttcaatttca ggtgtgtacg ttaattctga ccgactttgt acttctttct tttgtaattg
     8701 ttggatttca tctttcaaaa acgacacttg aaccttcgtt ttcatggatt gaaaaaatgc
     8761 caagccccct gaaaaacaaa gaaataaaac caaacaccaa atccatttca ctgctttttt
     8821 tgaatggatt ttcttttgct tcggtacttt ctctgtataa actttttctt tccgccgtag
     8881 ttgtcgtttt ttcataagct actccttcta gtatgttttt actagtacgt acgattgaca
     8941 acgataagct tgtttttctg aaaaatagtc cattcaatta ccctcatttt ctattccttt
     9001 tctctttctt ttatatccaa caaatccagc aatacttgtc aacactcctc ctagaacaga
     9061 aagtgcaaca tgtatttctt caccagtctt tggtaagact tttgtttttc catctgctgt
     9121 tttgatttca atattgccac ttggaagttt ctttacagga atcgacaaat cctttaccaa
     9181 attcccttca ctatcttgga ttaatggaat accatctttt acaccaacaa ttgtttgatc
     9241 acctgctact tttaatgggg tggttggtgt tggaatatgg gtcaattcct ctacattact
     9301 agtgacaatc ggtacactca ttccttgaga agtattcgtc gtaatttcac cgcttggggt
     9361 taccgatact gtgacttctt ttgctggaac aataggttta ttttcttttg gaattgcttc
     9421 tgatttttca acaggttgtt tcggttcttc tttttgttct tcactggtag acgacatcgt
     9481 tggctgttcc gtacttgacg aatcagtatt cttttcagaa gaactggttg ttcctgattg
     9541 ttccggagtt gtgactatac ttgaatccgt tgttgttgaa ctatgcacac cttcttcttc
     9601 gatcgaatca gtcggtaaag ttgattcagg aaactctggt tctggtgtca ctggctcttt
     9661 ttcaggtttc tctgactgca attgttcagg ttctgctggt ttttccgttg ttcctccatt
     9721 tagctctgtc tcaacatctg gtaatccttc ccctggcaat gtttctacaa tttctgactt
     9781 aggtgtttct acagtttcat ctgcataaac caatgaactc cctaattgaa acgaaaataa
     9841 tgaaattcct agtgttgtta cgactgctaa tgttgttttt ttcatgatta ttcctccaat
     9901 tttttatgca attcgtgttt tgttcataga cgccgcacta atattatctg aacgataaaa
     9961 gaactcccgt ccgttttctt ctaacccaac atatggatta tttttgaata attgaatcaa
    10021 tagttcactg gtgttgtatt ttggatagtt tgcaaaaaaa taatactgtg cttgtcgccc
    10081 atccgaaaat tcaacaagta attcataacg ataccgcttc aatccttttc gttgttttaa
    10141 aggatctcta taccaacttg tccatcgttc cattcgtgga tagagataga cacgatacac
    10201 atagaacaat gccataccac ataccaccaa aaataataat cgccacataa aaattccccc
    10261 ttttcttcta cctttcttga atcatcgaac attcataccc tctatagaga tttgaaaaat
    10321 ccatcatcaa ttgttcatat aaacgttgtt gcaaatagcg attgtttcga atacataatt
    10381 cttcatagcc atcaatcact tgatcttctt tttcatagca tcttgtagct ctatgacgct
    10441 cagcttgcat gacataacca aactctgcac catatcgttc tactgttaat aactgttgac
    10501 tacgcaataa tgcaatataa caatcacaaa aatgttcaac cgacatttga tttttctcac
    10561 gtttgtgaaa taggtattct ttagaaagca cattattcgt taaacaatct gataaatagc
    10621 gataatctgg cggtcggtta ttttcatctg tgaatttttc ccataataaa ctgattttta
    10681 ttcgttcagc ggctagatag tcttgttcgc gtggataaaa ttcatcaatc gcacaaagtt
    10741 ttttatcacc tgaataaaca aatccatata atgcgaaacg aactcttcct tttcaattgg
    10801 ctctcctaaa aattccagtg acataccatc ttctccttcc taaaaaaaat aaaaaagcag
    10861 aaaccgattt ggcttctact tttctttcct tttatagctc ttttttacgg aagaatccgc
    10921 aaatgacatt ttttcaaaaa atcaatcggc acaagttcac gaaaacttgg agcaattttg
    10981 gatagaatca cttccccatc ttgtaaaata caatcataaa aatcatcagt tacttgatac
    11041 ttacgcttct tacaataatt gataaaatag tagcgatcat tttctatttc cattcgttgt
    11101 tctactaagc gcatacgaac atcatcatag aaataagcca caaatcgccc ttcaagttca
    11161 tgtttttcaa tgggatactc ttctggtaac aatctatttt ttcgtcgttt atacattgct
    11221 ggatacctta gaagaagttc atctaaacgt ctgtcagtat atggtaactg acgaatcaac
    11281 gattctaatc gttctttata cagatagaat aattggcgca agcaatagtg taccgcctct
    11341 ggattttcta atactgttgg atcacgtgac caatacgcac caacataaat cataattttt
    11401 ttcatttaga caatcacacc agcaatacta atgaccgatc cacaataggt agaacaagtg
    11461 gcatagcgac caatttcctc taattctatc tcaatctctt ctaaagaaag atatgtctgt
    11521 tcttctgttg gctggcgact catttcccaa gtgacagtca ccaatgaaat ttcttccata
    11581 agaggttttt cgctgctaag tgctgccaca cagttttcat atatcttcat tttatcgcag
    11641 ccttttttaa ccgctaaaat cgcaggtatt ggcaatgaaa atcgataaaa gcgaatgaat
    11701 tctagccttc ctttccttcg gctaaattcc aactcttcga gttcttgttc gtatccctta
    11761 cgaacttcta ataagcgtgc aatttcttgc cagttctctt tttcctgata acgtccagct
    11821 tttaccgctt gttcaatata aggaactcga aaacgaaaat aagacaaatc aaccatcttt
    11881 tcatttaatg cttcgattgc agcctttaaa tccaacaggc gcagccattc tgttgtcgta
    11941 tatgcattca tgcaattttc cctcctgacg ttcccatcca ttctagatag ttttgttgct
    12001 cgtcatttag ttcggcatta tcaatccctt cttgtacaaa gtctcttcct aaaatacgat
    12061 ctgcttcttg taacaacttt aatgtcggag ctacttgttt caaaaaccag cgttctgaac
    12121 gatctaatga gaatttttct ggctttactg taaaatccaa ttcttccatc gaacgaaata
    12181 attttgccca tcgtttatca acaatccgtg catcactttc cagtctatcc caaacaataa
    12241 gttttgcatt aattaaacca acgccaatct ctcctaaacg tttcggtgaa tcacctaaaa
    12301 taaatgcttc tactgcttgt gttgctttct cattggctaa ccgtaactca tatcgattat
    12361 aaataccata tttcgttaat gcttctaatt ctgaaatttt ttctcggttc gcaatttcaa
    12421 aacgtttctc ataaaaatta aaaaagagag gactctttcg cgatccaaaa tacaaagata
    12481 atccttcact tcgaaaatag ccatcatcta aaaaacaacc gccaatattc tggaatctct
    12541 taaagtccat ttctaataaa cctgcttttt gtttttctag taattcaaag agatcgaaat
    12601 gttcttccgt ttccaatccc caaagttcat ctaacgcaat atcgaatcgg ctacattgca
    12661 cacgtgtata accagcttcc aactttcctt gatttttcca aatggaatat agccatgttc
    12721 gccaatctgt ttgttgttca cgcatgactt cctcatattc ccggcaacct tgtcctgaaa
    12781 attgcacaag tacattttct gttacagttc gttttgaatg ccagaacacc cagatattcc
    12841 cacgaacaga aagacagtca aaaagataca agcccgttgg acgtgtttca aattctgtca
    12901 gatccattcg taatacacgt tgaatcactg ttttgtagtc tgtcccaaaa aaggaaagac
    12961 gcaaataatc aatcattgct cgaagatcta ttccgctttt catatacaat cgaaagcgtt
    13021 ccttgattcg ttgttcaaaa tcctctggga tttttctttt cccttgttcc atctttctaa
    13081 tgtaatcttt tgagcagccc atcaattgcg caaatcttgc ttgtgatagc ggctttttat
    13141 ctccacttat attatgaatt gcacttaact tctttctgta gctttttagc tcaatcggtg
    13201 tcattttctc gctcctttta taaaataaca aaatcgattt tttttaaccc tttagaaaca
    13261 ccgatatatc aactagtccc cctcgttttt tgggaaaagt agtacaactg ctgaccttag
    13321 ataatcactt gatacaaaag ggtttataaa acttttttct atgtgttatg ttatttctgc
    13381 cccccatatt atatatcggg gggctttaca caaagaggcc gtgcgcaagc gcacgacctc
    13441 tttgtgagct gcacacgctg ctagcgagtg cgcattatag gcccatgctt cgcagcctta
    13501 atgcgcaccg ccaggcagag tgcggctacc agactacgtc tagagccctc ccgatagata
    13561 ataactacaa aattaaatga tctaattttg ttatttcact atatttttca ggagaaaaaa
    13621 cgcgataaat tgacaaagtt aaatcctctt ctaaatctac tcgataatga tacaatcgag
    13681 tcaatcgttc tatatctttt ttcgttagca atacttgatt aataactttt cctgtaaaaa
    13741 cacgttcttt gcgcaaaaag cggtaatcag ttacttgttc aatacgattc aaccaatcat
    13801 aaaacgttct ggaagaaaga gaaggattaa tcattcgtat tgctgtagct actctataat
    13861 aacaactctc tgccatttaa cttcacctcc ttagttgatt acaaaatatg cgcatcagaa
    13921 aaacttgttc cttttggatc ttttgctaaa tacatttggc gctctagttg ttcttgtaac
    13981 aactgattat catctcccat cgtttgatct tgactatcct cttttactgt aaaatcatag
    14041 acaatcattt cagtaaattt ctcaaattct ttgataaagt gataatcttt gtaaacaaat
    14101 ggcgcaaaaa actctacagg tacatcaaat cctggaaaag catatccacg accaggttga
    14161 tctttgttgt aaaatggctt atttttaaaa tcttctccaa aagtcattaa ataaccaatg
    14221 ggagacaatc gaccaacaga aatacggaat gttaaattat cccgaacacc acttggaaaa
    14281 tattctgcat ctggtcgttg cattgctaaa ataatgtaca tccctgcttg acgacccttt
    14341 aatactaatg gctgaatcaa tttatcgact ctatttctcg cttccatatc caacgaacca
    14401 taaaaagctg cccattcatc aaaaaagata aaatgcggtg gtaacccata ataagcataa
    14461 tttttccctg gttcatattt aggatctttc atggttttca tataggcata ccgttgattc
    14521 atttctttta atgcatttcg taaacaaata atcatttctt catttatttt gtaaaacaca
    14581 tgtcctttga atacatcgac gtcacctaat gctgctaaat ctgccgcttt tccatcgcaa
    14641 atatctactg tcccaacact taaacaagct tgaattaaag aatacaataa ataggttttc
    14701 ccaccaccga ttgcaccagc aatcaacata tttggtgatt gatcatatac ccaatccaaa
    14761 gttttcatta aatgaatacg tccattttct gctatacatt gatcaatagg aatccgacca
    14821 gaatctgaaa ggaatacata attgattcgg gctttttctc gttcttcacg aattaaatca
    14881 cgcaaaaata cttcagataa caaatgaccc atttttaaaa atcgttcgtg aaaacgccca
    14941 ccatctaaag gaaaagaaac cactaattct tgtttctttt gataaaaata aacttttggt
    15001 aatctcattt tttcccttgt ttgtttctca ctaacttgtt tctttgtata aacaaaccga
    15061 ttgtcaatta acattcgaac aagtaactga cgccaataaa cagaggcaaa atatccttct
    15121 tttagtcgcg accaatgcat caaccctaaa attaacccac taaccagcaa caaatacaac
    15181 acaatactta ttccaggaat aagatacatt tgccaattcg aaaacgtact taaacgtgga
    15241 taaaatactt tccaactaaa ccaacctaac agaatcaaaa atggaaacaa aaagaaacca
    15301 taatagcaaa accataaata acgattgaaa tagcgaattc gccgaccacg atacttgaaa
    15361 aaatgagctt tcaattgtca cattcctcct ttctgttttt acttttcctg tgctaaaaac
    15421 ttttgacaaa aagcaactaa tacaggaata tcacatttag taaaaaaaca taatcgaaaa
    15481 agatttaatc gatttttata agggtgtgca cacatattac atattcgtac tatacgaatc
    15541 caaatcactt cctgatgatc ttgtttccac tggtacggta atgaaaaacg aacaagatca
    15601 cgtaaaaaca tacactgccc aataaccgca caaatatatg catttgtttg tacaaaaatc
    15661 gttggattgt ctccaaaata ctcaatcaat tgttggatat tttgataaca agcacaatct
    15721 ctcaaagtat ttgattggtt ttatctgaat tcaaaatctc cccttccttc tatcttagat
    15781 aaaaagaata ggaagactcc ttcctattct ttcaaaaaaa ccttattttt tctctttttc
    15841 cttagaatca acacttggtt gtttttgatc taaacctttt tcttgtgatt gtttcatatt
    15901 aggattattt gttggagctt ctactttctc taatcctttc gcataaatca ctaaatctga
    15961 atattcacga ttgccatctt gtgctggaat atataccaca tgatattctg gatcaattaa
    16021 tttcacagga tcacgtcgtt taaaaccagt caaatctaca tctgctggaa atttaactcg
    16081 tcgtgcaatc tttggttcat ctggatttcc aaaaactaat tgtgctcgct gtgatcgaat
    16141 ttctcccgta taatcgcctg tttcattatc aaatacacga tcagactctt ctaatgcgcc
    16201 aaagcgtacg gctcctaaac aagtttcatc taatgcttct gtaatgtcat aattatttgg
    16261 aaattttaat cccatcttac aaaacctccc caatttttac accagttaaa aagacaccaa
    16321 catcttgata gccagttgtt cgacgctctg tttgtcccat atcatttgtt tcacgactat
    16381 tcactcgttc tttacgaaaa acaaaataag cttcttcaaa tgaaatacgc acctcttttt
    16441 ctggtaatcg taatctttta tcctcttcag ctgtgagata aacaaaaaaa gcaggctgtt
    16501 cttctcgcga acactctgct tgtaattgaa catatcgccc agacacacca ttaatttcct
    16561 ttggtggatt aggttcccgt gaacccaata aaaatacttg acctaaaaac tctttcttca
    16621 tacgcaatgc tttttctgcc ataaagtttt tcctcctctt aaaaaccaaa ataaattcta
    16681 aatatttcta cattacaatc aaacaagcgg ttaaaaatcc taaccgcttg ttcctcatga
    16741 ctaatctttg gctttacgta ctttataaag tgttatacct aaagcaatga ctaatactac
    16801 taatccaaga accataagta tactacttgt ttgttcacct gtttttggca acattggtac
    16861 aacagtagct ggtttaaccc gttgttctgg tttcactggt tgcggtggtt ttggcgcttc
    16921 cacaatttga accgtttgat ctttgtttgt tagatctaca ttgtgtttcg ccacttcttc
    16981 aaacgtttct ttctcatctt ccgttttcgc ttggagccat tccgcaaaac tcaagccttt
    17041 tcctgcaaga ttgcgaccgt ccactaagaa ttcaaataag aattccatca tttctttatc
    17101 ggccgcaaag actttttccg catgagtgcc ttcaaatggt gtgccgtctg gtgttaagta
    17161 gccttgaaca attaagcgat agatttgacc aatcgcaaga ccgtctactt tgacccattc
    17221 aagcacagtt tgatttggca aggccaagat ctcttttccg ccatttttat tcgtcgcttt
    17281 cgtcgtaatg gttggattac tgattttgat ggtttggtca ttgtcggtaa tatctgcgtg
    17341 agaagccaat aatacgcctt ctctaaacag atcttcaaag accacgactt ctttgcctcg
    17401 caacgcacgt gcgtctagaa taaagtctag attgactgta ccatccgttg tttctgccac
    17461 aaacgttaag gtacttgtga cttcttttcc atctactagc actggttttt ccgttacttt
    17521 gtccattaga acaccgttga ctgtgtatgt ttttccaacg accaaatcgt ggtatttcac
    17581 agcgtctgta atggtgactt tcgttaatgg attaacctct ttcaagccag tgggtttatg
    17641 tgtagccgtt gtacgtacac ttgggttagt tactcgtacc gtttgccctt tgtcgttgat
    17701 gtctttatgg tttgctagtt cttcatcatt tctaaaaaga ctttcaaaca cgacgatttc
    17761 acgaccattt atacctgtca aatcaaaggt aaatggtacg atcaccgttt cattttctgc
    17821 tttggcaata aagccaacac tcgtttctac cgtatttcca tgcacaagca agggttcacc
    17881 ggttgcttta tccattaaga tccctttgac caagtatgct tttcctggaa gaatctcggt
    17941 aatttcaacg gtatcgttta aggtaaacaa tcctgttggg tctgcgactg tcaatccgtc
    18001 ttcaaagaca gccgtggtat gaacggttgg attattcact ttcacacttt gggcttcatc
    18061 gttgatatcc gcatgtgctg caacttcaat gccatcacgg aaaagttttt caaaaacaac
    18121 gatcgtttga cctttcagaa cactagcatt aaccacaaat tcaaggtctt ttgtgccatt
    18181 gactttgtct gcaataaagg ccaaggtact tgtgatttct ttgccatctt gtaaaattgg
    18241 cgcattcgtt gccttgttca tcaatactcc tgaaatggtg tattcttttc cgacaaacaa
    18301 gttggtataa gaaacactgt ctttaatgac gatcgtttct aatggatcaa ccactttttc
    18361 accatcggct ttatttgacg cttgtgtttc tagttttggt ggaatcaagt aattacgtac
    18421 agaaaccact aacgtttccc catcatctgg cgcattttgt ttgaatgcaa ccgtgaatgc
    18481 ttgtttatct gtatttggtt ggaaactttc taatggggca ttttccaaga agtaatagtc
    18541 atcggcaact agacctttca ctaaaatttc cccgttttga tcagtggtat attcaccaat
    18601 tgcttgattt ttaccgttat acaaggtaaa gttcacacct gcgagcggcg ttttcttctt
    18661 gttccattct tcatacttga ataattttac acttttccgt gcttgacggt tcgcaaaatg
    18721 gatcactttg atcggttctg tatcattggt aatcgagaac actaatgggt tttgattcaa
    18781 aacgtaattt acgggggctt tgatttcttc caaacgatag cgatcttttc cataagtaag
    18841 tgtaccgtta gttaggaaaa agcctttttc attggtttta aagatatccg ttgtttctgt
    18901 atcatttgga atctccattg tgacataacg attggctaaa gtatcaaaaa tcttgaattc
    18961 tgtattagca agtggaatca cttttcctgt ttcttgatcc actttcacca ctttaagacg
    19021 ttgttcaatt actttatctt ctaaaacata atggaaagtt tgcccatttt cagaaattgt
    19081 gacttcaagt ggttcaaata gtttataccc ttcttttcct ttggtttcag aaagaatgta
    19141 cgtatcaaag acaacggtat ttttaccaaa agtagcataa ccatcattgt ctgtcacaac
    19201 ggttttcacc actttgccag ttgtttttga cgtcaaagtg atttcaatgc cagctaattt
    19261 tggtttgtta tctggattcg ttgaatcgac taaatcttta ttagcaactt tcactaaatc
    19321 aatggttcct ttgatcactt gatcttttgc ggtcgcattg acagtttttc caggttcaac
    19381 aacaatttca attgcttcat cagaaagcac ataacctttt ggcgctttga tttcttttaa
    19441 aacatatttg ccaggatcaa gatctttaat ttctgcctgt actttgccgt taattttttt
    19501 caaggtaatt tctttaatct ttttcccatc tgcttgatac aaaccataga ccgcattttc
    19561 taaagaagct gcaccttgtg ctttcacacc agtttccgca tcttctttaa acacacgaat
    19621 gtttccttgt tttagaattt gtaaattaat aacagacgca ttaggttctg ttggatgtaa
    19681 cacaccaatt gtttgagctc ctggacgacg ataggctaat ggcgcacctt gaactttaat
    19741 tttattcaat tgaatttttg ctttatccgg tgcctctttt gtgactgtaa tccatacttc
    19801 attgccgtga atagatacat tgacaccttt tggtttttga atgtctttca catatttcaa
    19861 aaaatcattt ttattcttta ctttcaacgt ttcgccaatt tttaccgtat gtgtttgatt
    19921 attaaaagtt gctttttcac caaagttatt aatcaaacgt tgaatttctg cttttcgacg
    19981 atcataatca acacggatat ttttgattgg gatttttgtg cccaaagctt cccaaatcat
    20041 aaattgtgcc gcaatgtatt gttcatcgga tttgtcttta tttgtgacat atcctaaata
    20101 agagatcaag cttgtttttt tccgtacatt tgaatttttc aaatagtttt tcaaagcagt
    20161 agctgtataa acttgacctt ctgcggctga gacaccaggc tcaatacaaa acgccacttg
    20221 tccattaatc tcaaaacgaa ccatataatt taaagtgaag tttagattgt tcggactaga
    20281 agtaacacca atattttcgc cccaatcaat tttccgacca cctaaaacag ctgcattggt
    20341 tgcacggcga acacgaaccg aacgattggt tggtactgtt actaacagtg taggtgtacc
    20401 tgtttttgga atgttctgct cttttttctt ttgattttca atactagccg tagattcttt
    20461 gtttggtttc gttggttgat tttgactagc tgcggttgaa gagattgttt tttcaacgtt
    20521 ttcctcattt tttccttctt tgtcagcagt agccgctggt tgttccactg tttcttctat
    20581 tcgtttcttt tcttccgctt gtttctcaga agactctggc tgcacagaag gcgttgcttc
    20641 ggcagctttt tctccttcta gagtggacgt ttctgaagca ggggttgtaa ttgaggcagt
    20701 ttcttcaact ggttgtgaag taccaggact attccctggg tgttgtgttt ccccaatcgt
    20761 ctgatcgaga gtcgttttgg aattgaccgt tggcgcttct gtcacagggg cctgttcaac
    20821 agtggcttca ccagctggga ccccggcttc tgctggttgg acttctgtca cttcagaagc
    20881 gacttgggca atggcttcca tcgtaggttg tatagccgtc gtttcttctg catgtgcttg
    20941 agcagttccc accaagaaac agaattgaga aaaagccacc aatgcgagtg ctttcaacca
    21001 attcattttc gtttttgcat acacaagaat tcctcctttt cctgagaatt ttatataata
    21061 aaacaaaacg ccgttaccaa aagtgataac gacgtgaact gtctcttcta tttttatctg
    21121 gcgctcttat ctgaacacat ttcatcaatg attttaccct cccactataa ttacagaaca
    21181 aaaaaagaac ctgcttcgtg gaaatcaact gtccatttcc caattggaat accagctttt
    21241 ttggcaactt caccatcacc aaatctcatg ccataccaat tactaccttc atccttaatt
    21301 tctttccaac catatgacat agcttcatct aatgaatcaa aaatcatgac actattgcca
    21361 attgttacag gatctggctt aagatcttct ggaagagaaa tatcaatcat accataatga
    21421 tccgattcct cagtattatt gtcattacta gctactggtt tggttgttct actggcttat
    21481 tttgatacaa caagtactct tattcttctt ttacttctcc gttttcaatt tcccattcaa
    21541 accactccgc aaagtcttgc ttaaaaccat ctactcgata cctattttgt aaataggcat
    21601 cttctgacgt cgtaggatct aaataggtag gtttattctc tggacgagca tagacaccta
    21661 cccatgtttc gtaatctcct tcaaaataac tatctaccaa atagcctttt tttctaagaa
    21721 tctcccgaac ttgattttta attattgata cttgttcaaa accatcactc attctatcac
    21781 ctcaaataac tctagattct cataaatatt cccgacaatt tcaatcctat caatgatctc
    21841 taacaaatcg ctacttatgc ttccccattc gtataagaat ttcccttccg caaaataaat
    21901 tttccataaa cttcttcatg atcgtcccaa ccaatatcac tctcgaaaaa ttccacgttg
    21961 ttcttatctt ttagctcagt aaatgccata agttcaacat gttttccaac ctcgaaacaa
    22021 tcacaccacc catcaaccgt tacacgtgtg ccttcaatac ctaagaaact ttgtcctcgt
    22081 gtattaaaca ctaaaacttt ccgcattttt tgctcattaa ttaatcacgc gcgaaatcta
    22141 ggaatcacaa cttattcgct tcttctttat aaatcgccga ttgtaaaatt aagctagaca
    22201 actaaaaaaa gtcatttgtg ctacactcaa aatagttccg acgaaagaat tataaggagt
    22261 gagtacaaat gacctataac catcttacac caactgaact cgtaatgata gaagcttatt
    22321 tcaatcaatc acaacctgtt tcaaaagtag ccaatctgtt acaacgttct cgacaaacta
    22381 tttataaggt ctatcgtttt cttaaatctg gtggaagcgc aatgagctac tataaacaat
    22441 ataagaaaaa caaacgtaac tgcggaagac atccgattgt tttacctgat gagcaacagg
    22501 aatacattca aaaaaaggtt gttcaagggt gggctcctga tgtccttatt ggtcgagcag
    22561 agtttcctat tgcttgttcg gtacgcacac tgtatcgaat gtttaaagat aaaacctttg
    22621 acactcacga tttaccaatg aagggaaaaa gaaaaccaaa cggtcataaa gaaaaaagag
    22681 gaaaacaagg gtttcgccgg tcaatccgag acagaaagac agactatacc cagtttgatc
    22741 atgaatttgg tcacctggaa ggtgacacta tcgtgggtgg aaaacataaa agcgcggtca
    22801 tcacattagt tgaacggcta tctaaagtca tcattacctt gaaacccgaa ggcagacagg
    22861 ctaaagacat tgaaaatagg ctgaatcaat ggtttcgttg tgtcccaaaa aacttattta
    22921 aatccatcac atttgattgt ggaaaagaat tctcaaattg gaaaaagatt agtaatacca
    22981 acgatatttc aatttatttt gcggatccag gaacaccttc tcaacgagga ttgaatgaac
    23041 attcaaatgg cttattacgt aaagatggtt tgtataaagc tatggatttt aatggtgtat
    23101 ctgaaacctt tatccaatcg gtggcatcga aacgaaatca tattcctaga aaatcattac
    23161 actatcggac accaatggaa gtatttttga gttacgtcac aaaagatgat ttgtctaact
    23221 taatttgaca aacgaaactt aataaaaaag gaagaatatt catgtaaacg attttattgt
    23281 ttttttctct tttatacaaa aagggtcata atttcaagta agcggatgaa ttcatgagga
    23341 ttttttgcat actgtctcta ctatacttag gtcactaaga aaaaggagtg atttattatg
    23401 aaaaagaaca tgattgggac attattactt tcaacacttc ttctaggcgg cttggcaaca
    23461 ccagcttttg ctgagggtca agcgacttca aaaggagata ttacctttac tgaaccaaca
    23521 aataccgtgg agcctcttaa ccctactgat ccttcaaaac cagtagagcc agcagatccg
    23581 gaaaatccag caactggcca gacaggtcct ttaacactgg atgtagttcc cgaattgcca
    23641 tttggtacac acgagattga aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac
    23701 gatacacctt atctccaagt atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa
    23761 gggtggaacg tgacagtttc ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc
    23821 gctgaattag attttggaac atccactgtt aaaagtacgt cagataacga atccactgga
    23881 ccaactagtc aaactgtaac tgggttgagt aaagcaagtg cagcgacacc aatatttaca
    23941 gcagcaaaag atcaaggtct gggaacatgg ctttctgttt atgatccagc aaatatcaca
    24001 ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg atttgacttg gaacctcgtg
    24061 gctggcccag tagcatagag tatgaaataa attcaacaaa ctgcggtgaa aatactatag
    24121 gtgaaggatt ttttaccgca gttgttgttg aaaggatgtg gtggacaatg aatcaaagta
    24181 aacgactgtt tgtttcgttt ttttctattt taagtctatt tttcattttt ccgtcaatca
    24241 gccaagcaaa ggattcagca ggagactttg gaatcaaacc ggtatttcca gagaaccaaa
    24301 tagataaagc aatcggttat tttgatttat tagtggcacc ggaacaaaat cagacactag
    24361 aagtaattat cagcaattct tcagatgaag aacgaacatt tgaggtctct gtcaatccgg
    24421 cggtaacgag tgatggtgga acgatcgatt atagtcaaaa aaacccaacg ttagatgaga
    24481 cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag
    24541 cacatgcaga aacaactgta cctatagaag tgaaaatccc agcaaaaagc tttaagggac
    24601 gtgtattagc gggtattcat gtttcaccta aagaagaagc tgaaacagaa aatgctacag
    24661 aaggagcaca aatcaaaaat cgtattgctt ataatctcgc agtcgtttta caagaaagtc
    24721 aagagacgat agaaccggat ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa
    24781 agccaactgt tcagcttcgt ttccaaaatc ctcagcctag gattatcagc aatttgatat
    24841 ttacgagcaa gatcttttat gaaaatcagc tttatattga aaatacgtct aatgcatttt
    24901 tagttgcacc aaattcgaat tttcacttga atcttgattt agctggtgat aaagcaaaag
    24961 ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga cagtaatgaa tggcgattta
    25021 cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg
    25081 cagtacaaga acaatctttc ccatggcttt atgcaggatt ggttgctgca gttctagtgt
    25141 tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat
    25201 gatgaggtga tttcgttgaa aacatcgatc ttattgggag ggattattgc aagtttgctg
    25261 ttcttcagct ggcaagagcc agtccaagca gataccaaaa gttcaggtac gattacattt
    25321 tatgatctaa gtccaattcc gcatccaagt ggttcagtca atggcgcatc aaaacagcag
    25381 acacagctac cgatttataa agaatctaat caacatggcg tattcccgga gacgaatgaa
    25441 caaaagatcc gttttcttta tttgatcatt ggtatagaat tatggcttct tgcgtggttg
    25501 ttgttatgga gaagaaagga aaaggaggat gaacatgttg ctacaaaata aattttcatt
    25561 ttttcgctcc ttctttttta cttcgctttt tgttttaagt atatttatct tttcaccttc
    25621 ggcagaagct gttggagcaa cgcctcctgc catttcttat gatccataca cgtatgaaaa
    25681 tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc gggaagcttg tccaactaac
    25741 tggggctaac caattgccta aattgactgc tacttatttg aacgatggtt caaatgatcc
    25801 ttggaaaggt ggcacggtcc gttttcattg ggaaagccat gtgaaactaa caaatgtact
    25861 tgatttggtg aatatcactg cacaaggatt tgcatttgtg gcgatcatca atttgccgga
    25921 acatgtatca agcagtgatg tattaaaagc aattgactgg aacaacgcct acttgtatat
    25981 tgccggtgcc cccatcaagg ttactagcgg aagtttatta tcagcaggaa gccatacctt
    26041 gcgttttggg atggggtcgt gggatacaaa tactctattg acagatatta ttgctattat
    26101 tacgcgaggc gaattcaacg tggacgatat cccgcttcaa tttgatgtta cagctaatgt
    26161 cgcgaagatg acagaaaacg gagataaata tgatacgagt ccaaacaaaa ttttgaccaa
    26221 gggtaaatta caaccttcta tgtctaaaaa ggccgttttt tctgttgatt tttatgattc
    26281 gaataatatt atccaagatc gtatgttcaa ttcgatttta gctccagctg caggtagatt
    26341 ttatcctaaa ttaactggcg gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc
    26401 agagataaat acgtggaata gttacatctc accaagtgat tcaacaggaa aatataatac
    26461 atttgctgat accacagaat acgtggatgg aaataaccag aaccttccag gctctaccaa
    26521 tagtcggaca ttgacgatgg ggctgactga aggacgtttt gatcagttgc catttagtcg
    26581 ctttgatcgc gtagtcaact attttacagg aaaaaacgtt acacaaggag caatactgac
    26641 tcatactcca gcaagcacaa gtggattgaa tcagacagcc aatatcgtgt acagtgggaa
    26701 agatgcgcaa ggcggattgc tttctcctgt tgctttaaag gtattggagc agtacaagct
    26761 agacggaacg gttttaccaa caaacttaac agccgatagt cagtggggaa agctcctttc
    26821 ttctgcatcg taccaagtca cggccaattg gtcagcaact gaactaaaaa agggttcgat
    26881 ccattacagg ctttattcga aaggaaccca aaaattagcg acaggatgga cagatcaact
    26941 tttccaaacg atccaatcaa ataacggggg tgtaaataca gctacttcta cactacctgc
    27001 attggcttca gggcaatatt attttgatta tcgcttggtg gacgatgtac ttactaacat
    27061 gtatccgtct tttgcgtaca aatggcagtc tgaacaagct ggaatcacca ctcttcctca
    27121 aataacagtg gctaattttc catctgtgag tgcagatagt cagctaaaaa accttagcag
    27181 acaacctgag acaggagatc ccctgtctgc tctatcagga gatacgattc aagaaaatgt
    27241 gacattcacg ttaacaaaat ctggggatgc gataaccgac aaaaaaatca cgatctcact
    27301 tcctgaaaat actacttata tcaatggttc cttaaaatta aatggtacaa ctatcgcaga
    27361 taccggcatt caacaaggag tttctgtccc agcagatcta ttgagcaaaa taggagatac
    27421 cattcatctt acttacaact atcaattaaa tactgtagat acttctttac aatccgtcag
    27481 catactgaca aaggcagctg tactttccag caatattaca ttagcagatg gtgctaagct
    27541 tcctaaccca gttgtccaaa catctgctaa aacaattctt gtccccaaac aagaattaac
    27601 attagttaat gtaccagatg attttacatt tggtaacgat ctccccaaac cgttgaagac
    27661 aagttattat gaatcaaaag gcgatttttc ttttgatgtg cgggatactc ggcttccaag
    27721 cacgtctcct tggcaactga caggtacatt gacttcttta tttaagaatg atcaaggtca
    27781 agaattaagc ggaacaaaat tatacttcaa tcattctgga tcaaagcaat tgattcagca
    27841 aggtcaaaac acgttgattt atgagagtga tggaacagct aaaggagagg ttctcgttga
    27901 ctttcccgat accgatggtt tactcttaga ggtaaatagc agtacgaatg ctcaacctgg
    27961 cgcaacgtat caaggcatgg tcacatggga actgacagct gggccaacca gctaagtgat
    28021 tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg atgagaagga
    28081 aacttctgct gttaaaatca gaaatgcttg aaggagtgga cttggaactt ttcagtttgc
    28141 ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag gaaaaaggag
    28201 aggtttccgt taataacgta cttacccgct tatcagttga tcgggctacc ttgaaagaag
    28261 atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt gttatccaag
    28321 aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt tattatcatt
    28381 gttacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa aatggctcat
    28441 ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg tctcgcagag
    28501 tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc gggcggctga
    28561 ccggtccaga aagtcaaatt agttattttt atttccaatt attttggttt ggacgtccct
    28621 atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta gatctgctgc
    28681 aaaaagagtt tagttttgct attacagaag aaggacgatt gaaatttgaa atatggcttt
    28741 ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat agcaatcttc
    28801 ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt ttgacccgtt
    28861 acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt tatcttttca
    28921 tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt ttgataagcg
    28981 aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag
    29041 aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg ttgacagtga
    29101 tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt ggccaagaag
    29161 gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta tgtgaaagat
    29221 tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa aatgcccgct
    29281 ttgaattatt tgctcgctat gcatctattt tacggacgct gatcaagcag ttagaattac
    29341 gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc gtgaaagaaa
    29401 tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac gagaaagaaa
    29461 agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag aagtccgcta
    29521 aagtttttgt attcactagc aacgaatata attatgattt cccctatttg aaccaatttt
    29581 taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga aaatcgtgat
    29641 cattggtgct tcccatgaag gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa
    29701 agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg tttcaaatgg
    29761 aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc ataacatgcg
    29821 gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg aacttgatcc
    29881 agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca taacctatga
    29941 taaattaatc gtagcgacag gttcctctcc tgtcaccttg tataaacaaa ttgaagctga
    30001 aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg ctttaaaaga
    30061 agcaaaagaa gtagtgatct tcggtgctgg ttacattgga ttggaattag cagatgcttt
    30121 gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt tatcaagata
    30181 tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc aaataaattt
    30241 ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag tatccgtaca
    30301 acttctgtct caaacaataa aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa
    30361 tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga tagtcaatga
    30421 atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc cagtcagttt
    30481 tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac atatggcaag
    30541 agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac aaaagataac
    30601 agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag atgaagcgcc
    30661 attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc cacaatacga
    30721 cgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat tagaaataat
    30781 tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc ttgcggatat
    30841 cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat ttctgtctga
    30901 atacacctct cgttttcact acctcaatga gttagctttt aaagtactag ctaagagagc
    30961 aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta ttgtgtgggg
    31021 atataataaa tattaatcaa tcgagaataa aaaattcata taatgaaata gcggaagaat
    31081 tttttgagca taatattcgc caaaaattta tcaagtgata aaattgttgt ataaaatgat
    31141 tatttcagga ggaggagtta aatgttttta gcattaaatg aaattatgca ttcaaagtta
    31201 cgttatgcgt tagttgcagg ggtgatgttt ttgatcgcct acctggtatt ttttttgact
    31261 gggttagctt atggcttagc tcaagataat cgaacagcag tcgacaagtg ggaggcagat
    31321 tcgattgtgc tttcgaaaga tgcaaattca aatctaggga tgtccatgat tactaagaag
    31381 atagcagaag aagtcgaagg tgggaaagtg gcgtatcttg cccaaacacc tggagtcgtg
    31441 actagtaaag acagtacaga agaaggaaaa atcaatgtta gcttcttcgg tatcgataaa
    31501 aatcaattca tcatgcccaa tcttgttgaa ggaaaagctt ttgataacga tgatgaggca
    31561 gtaggcgata ttagtttgaa agaagaatat ggtctagcca taggagatac agttaagtta
    31621 tctggtagtg acaagacgtt taaactaact ggctttacgg atcatgccaa attcaatgtc
    31681 tcaccagttc tctatacaac catcaatgct taccaagaaa tccgcttcga aaaagaagat
    31741 acaagtgaaa atgcacggat taacgcaata gttgtcagag gtaaaatcag taatttacct
    31801 gaagatttag aacagatcaa aatttctaaa ttcatcaatg aattacctgg atataatgcg
    31861 caagtattga cttttggatt tatgatcgga tttttgatcg ttattgcggc tattgtgatc
    31921 gggattttca tttatgtact gacgatgcaa aagattaata tctttggtgt aatgaaagca
    31981 caagggatca caggaggatt tattgcgcgt tctgtagtgg cacaaacatt cattttatca
    32041 tttgttggta ttcttcttgg tctattagga acagtgggaa catcccttgt tctacctgat
    32101 gcggttccat tccaaagcaa ttggctgttt tttggcgtca ttagcttgtt gatgttagta
    32161 gtagctgtgt taggcgcact attttctgtt cggacaatcg taaaaatcga tccgttgaag
    32221 gcaattggat aagaaggagg aagaacatga aagcaataga atttaaagaa gtagaaaaaa
    32281 gtttccaaga tggcgaccaa gtagttaaag cattgaaaga aacaaccatg agtatcgata
    32341 aaggggaatt tgttgcaatc atcggtcctt ctggttcagg taaaagtact tttttgactt
    32401 tggcaggcgg attgcagacg cctagcagtg gctctgtatt tatcaatgat caagaattca
    32461 gtaatcaaaa agaaagcgaa cgcgttaaac tacgttttaa agaaattggc tttattttgc
    32521 aagcttccaa tctggttcct tttctgacag taaaaaagca gttattatta gtagataaga
    32581 ttaaacatga acatcgtcaa aaagaagcaa acgaattatt tgagcagctt ggcgtggata
    32641 agttgatcaa caagtatcca gaagaactat ctggtggaga acggcaaagg gtcgcaatcg
    32701 ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga tgaaccaact gctagtttgg
    32761 attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa agaatcaaaa gacaaaaata
    32821 aagcaacgat catggttact cacgatacac ggctgattga caaatgcgat agaatattta
    32881 tgatggaaga tggtgtattg aaagaaaaag aataaccaaa aagcaggtgg aaaaatggtc
    32941 catcgataca acaaagatac acagcaaaaa attttgaaag aagcgactga cttgtttatg
    33001 tccaagggat atctcggcac ttcaacaaga gaaatcgcac aaaaagttgg gatcacccag
    33061 cccaatctct atcattattt cggtgataaa gaaaaactat atacggcagt tttagaaaac
    33121 cacttaaaag atgttggaaa agctttgaga gaaatcgttc aaacaagcga aacgggattt
    33181 caagtaacct tgacaaaaat ggcgcagttt ttgattgaga cacatctggt cgatttgttt
    33241 ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa caagagatca tcttttcttt
    33301 ctatggaaga aaaattatcg tgaacctttt gaagaaatct tttcagcaaa ccaatctgtg
    33361 ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt ttttcttagt gttagcacct
    33421 tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa atgtggcgca attgatcgat
    33481 ttatatttga atggtgtatt aggagaagcg taaagctgtt atatagggag aaaatagtta
    33541 ggtgaagaag caagatatga cgaagatcgt tatatcttgc ttctttatat aggtagtttt
    33601 tcataaatac aaatttgtta ttctctcatg attatcaggg tatattttta tttattatgt
    33661 cggtgttcca ggaagtgttt taatttaata acgaagaaat taattgtttg tagaatgaag
    33721 gaaactattt attgggtaga tgaatcacca cgaatagtca aaaggagatt tcaggatatg
    33781 ccaaaaatga aaaaaatcat tttagcagta atgttatttt ctacggttat atcttttaca
    33841 gccccttttt tgcttactta agctgatttt aagttttggc ccttagtctg gatctacgag
    33901 gtgagagtca agaatgaaaa aaataattgg agcttttgta tttgcaatca ttatcattat
    33961 tatctgtttt caaatagatg tttttggtca aaaaaagata gttggacaaa cggtcagcag
    34021 tacgaacagt acggttacaa agacaaagga atcatttgat gatacagaac aagagacatt
    34081 aatcaaaatc ggaaaaaatg accgtacact atatacagat gactcgctaa ctaccccact
    34141 tgcaactata aacggcggag agctggcgga gtttctttct gaaacaaaaa atgcttatca
    34201 gatcaagaca aatgatggct atactggata tttagccctc gaagatggaa ccaaagtcac
    34261 aaagaatata caaacaaaac cggaagagct atctgatgca gtgattgtat tagatcctgg
    34321 gcatggcgga aatgatactg gggcattaag caatgatgaa caaacagaag aaaaagatat
    34381 aacattaagt acagccataa aagtaaaaaa ggcgctagag gatgctggag caacggttta
    34441 tttgacccat actacagatg aattagtaca gcttggagat atatgcgact acagtgaaga
    34501 aaaaaaggca gatgtcttta tcagtttaca cgcagactct acggaatatg cgaatgaagc
    34561 aactggaatc acgacatatt attactatgg acaagaggaa acattagcac agacaattgc
    34621 tgactccttc actgatctgc ctctggattc aagaggaatc tccactggga actatcaagt
    34681 tcttcgagaa aatcttcaac cttctatttt aatcgagatg ggttatatga ataatgatag
    34741 tgatttaaag gaattagtaa cagacagtta tcagcagcag attgctgcaa gtttgacaca
    34801 agcattgacc acttattttc agtaataaat aagcccagcc aaacaaaaat agctcctcat
    34861 atttttcgtt tggccgggct tattatcgca gatgcaagct tttaaacacc gtttaaggaa
    34921 gaggtcgtga gcctgccgtt caactctgag tcacaaggag caatttcaaa aaacagcttc
    34981 tcatgtttga tgactattgt gacttgtgac cgaggagttg gcatgagaac accgtttaag
    35041 aaaaaggttg tgaaatcaac gttttgttct gagtattaga gaaacaaacg gaaaaatagt
    35101 tcctcatatt ttgccgtttg ttgagctaat accgaggatc agatgattga acaccgttta
    35161 ttcggaacaa gggactgtga catgactttt gtcacagttc cattattttt ttagtagctt
    35221 caaagctttt tctgtattgg caaaatgaag tttggcatat ttttcaagca taggcatgcc
    35281 gcctttctcc aatagttttg ccgctacttg gtcagatttc gttccagctc ctgacggaag
    35341 ggtgattcca gcttcagctg atgctttgtc taactcttcc aaaaagctgg cacgactgat
    35401 gatagaagca gcagcaacag ctaaatgata ttgttctcct ttagtaacga agtacagttt
    35461 ttcttgtaat tgattttttt ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa
    35521 ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt ttttttagca gcaaatggat
    35581 cgcttgattg tgcaaagcga ctttcatccg aacggcattg tattttggtt ggatctcatt
    35641 gtatttttct ggagataaga caagcagttt ataggggatg gtttttttta agatgtcagc
    35701 aagctggatg atttggctat cttttaactc ttttgaatct ctaacaccaa gtttacgtaa
    35761 ttctgcaagc tgatcacgtt tcacataagc agcacatact gttagcggac cgaaatagct
    35821 gccatttccg acctcatcac tacctaaaac ggataattga ctgatatttt ttggcaaaga
    35881 agaggctgtt tttgctggct tggcttgttt atcctgtgcc tccccccatt tatcagcttc
    35941 tattgctgca ttcgtacctt gaaacatcac ttttccggat gtataagcgg taatggtcgt
    36001 cccgtctttt tttgcagcaa agctagtata aggaatctgt ttatttaaca aataagagcg
    36061 atagtatgct ttcatttgac taagttggtc gtttgttgct ttgataatga cgttgctcat
    36121 aaaattctcc tcgtttttat acataaaact ataccgcaac tcacgacttt tcgttttcta
    36181 agagattgcg agacatagca tttttttaaa gtttatcata taatagttaa gtatgtgaaa
    36241 aatatttgtg gagaacggtg aaagaaccaa cttcatggta cactaaataa aactttcatc
    36301 aaataggagg aaatgattcg tatggcgcac gaaaaaacac gctacaaagc ggtgattgcc
    36361 aaccaaacat atactattat cggacgggaa accaagcatc atatggatat cgtaacgaag
    36421 ctgatcaatg aacagttagc agaattgaaa caattatcac cacaaatgga taatgaacaa
    36481 gcagcaatct tgatggctgt caatgcgctt tcggatcaat tgaagaaaca agaaagaata
    36541 ttggagctgg aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt tacagagctt
    36601 gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa
    36661 aatggtcaag cagatcatac cattaaaaat catgtggaag cacaacagat tttaaacgag
    36721 aaacgaaaag gacaaattaa acaaaaatct tctaattaag atccttttat caaaataaga
    36781 aaaatttaaa aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt
    36841 ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc
    36901 gcgattggat atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt
    36961 catttggaat tatacattcc atacccagct gtcaccccaa catcaaagtt agcatttttt
    37021 gatcaggtat ttgcctttca cctagatgaa gctttttatg caggaactgc ttttctgatt
    37081 attttgctaa ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg
    37141 tatgtgccga tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg
    37201 gcttacgtta ctatattttt gattttgcag ttacttgcct ttgttccatt agatgtggtc
    37261 caaaatcaat tcggcgagca tagtttcgct cgttttatcg ttgagagaac gccatttctt
    37321 accaacaaga tgtatgagct ttgggtaacg aatattttga attaaggaaa atagtgctgg
    37381 aaaatatttc gttttttgta cagagtagtt tatttaagaa ggctgcgtag cgtatgtccg
    37441 gcacagctct tttttcatag tgaaagataa ggtgataaaa tgaataaccg aatccttgaa
    37501 acattggaat ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac tgcacaagga
    37561 aaagaagaac tggcagaatt ggtaccagtg agtgagaaac aaacgatcac aaactggctt
    37621 caagaaactg aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa
    37681 attgaaaata tccgtcccca tatgaaacgg attgagatcg gagcagactt gaatggagtg
    37741 gagcttgcac aagtttccag agtattgtct acgacaagcg aattgaatcg tttcattgat
    37801 gatctttctg acagtgaaat cgaatttgca cgtttgtata tgtgggcaga tcagttagtc
    37861 acgatccctg ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact
    37921 gatgacgctt ctcctgagtt aaagagcata agacagaaca ttcggcgaag tgaacaagct
    37981 gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg
    38041 atcatcacca tgcgaaatga ccgctacgtt atcccagtca aacaagaata ccgcggtgta
    38101 tttggcggtg tagtccacga tcaaagtgct tcaggtcaga ctttatttat tgaaccaaaa
    38161 caagtggtgg atttgaataa tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt
    38221 caacgtattt taagtgaact ttctgctgaa cttgttcccc atcgtcaaga aatcatccac
    38281 aatacttatg tgattggtaa aatggatctg atgaatgcaa aagcacgatt tggtaaagaa
    38341 gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga
    38401 catccactga tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg aaaagactat
    38461 caagccatcg tcattacagg acctaacaca ggtgggaaaa cgatcacgtt gaagacttta
    38521 ggtctgttac aactgatggg gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa
    38581 atcggtattt ttgaggaagt atttgcggat ataggagacg aacagtcgat tgagcaaagt
    38641 ctatcgacct tttcttctca tatgacgaac actgtagata ttttatcaaa agtcaatgaa
    38701 aaaagtctgg tattatttga cgagctcggt gcaggaacag atccgcaaga aggtgctgct
    38761 ctagcaatcg caatcttaga tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg
    38821 cattatcctg aattgaaagt ttatggatat aatcgggcaa atacgattaa tgccagcatg
    38881 gagtttgatg tggatacatt aagtccaacg tatcgtttgc tgattggcgt tcctggacga
    38941 agcaatgcgt ttgaaatctc tagccgatta ggtcttgata cagaagttat tgatgaagcg
    39001 aagcagttga tgaatgatga gagccaagat ttaaacgaaa tgatcacaga tttagagaat
    39061 cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa
    39121 gaacttcacg atgatttgaa agaagcttac agctatttct ttgaagaacg tgaaaaagaa
    39181 atggaaaaag caaagaaaaa agcaaatgaa gtcgtgtcag aggcggaaga aaaagcagaa
    39241 aaaatcattg ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag
    39301 catcagttga tcgatgcaaa gacccaatta gcaaatctac atcaagaaga aactttgaag
    39361 aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg
    39421 ttggttacga cttacggcca aagagggacg cttttacgta aaaacgggaa tcagtggcaa
    39481 gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg agctgacgcc tgttgctcct
    39541 caaaaagagc cgacacaacg agttatccat gctgtacgat ctgaatctag tagtcacgtg
    39601 ccgaatcaat tggaccttcg aggaaaacga tacgaagaag cgctaagtga ggtagatcaa
    39661 tatttggatt ctgccattct agctggttat cctcaagtga ctattgtcca cggaaaagga
    39721 acaggcgcac ttcgcaaggg aattactgat tatcttaaaa accatcggag cgtcaaaagc
    39781 tttgagtttg caccggcaaa ccaaggcgga aatggtgcga caattgttaa atttaagtaa
    39841 agcttactaa aggtaagcag atggttcgct atccaagtat agaaatgcta tactagtcat
    39901 agattttaaa aatggaggtc tttgatatgt cacaagtaat tacagataag acatttaatg
    39961 aagaaacaga taaaggactt gtattgattg atttttgggc aacttggtgt ggtccatgtc
    40021 gtatgcaagc accgattttg gatcagttgg aacaagaata tgatgaagaa gaattcagaa
    40081 ttgctaaaat ggatgtggat gaaaatccag aaactcctca acaattcggt atcatgagca
    40141 ttccaacatt aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt
    40201 caaaagaaca actacgtcaa atgatcgatc aatatttata aaaacttaca gcaaaaataa
    40261 gctgagggtg ggactgaatc cagttccgtc ctcggttttt ttgcggttga gttaaggtta
    40321 ttcaaattaa tattttttga gtataattac gttgtcaggc tggattgttt ttttgacaga
    40381 catgctataa taactttaat tgaatatgaa ggggagtacc agatgaacga acgaatcaaa
    40441 aacaaattag ctttacttcc tgatcagccc ggctgttact tgatgaaaga taaaaatgga
    40501 acgatcattt atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc atattttact
    40561 ggtagccata atacgaaaac ggaacggctt gttagtgaaa tcgaagactt tgaatacatc
    40621 gttacagaat cgaatatcga agcattgctc ttagagatca atctgataaa gaaaaatgat
    40681 cctaaataca acattatgtt aaaggatgat aaaacctatc ctttccttaa gataacgaat
    40741 gaaaaatatc cccgtttggt gattactcgt aaggtactaa aagacaaagc actgtatttt
    40801 ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga tcgattattt
    40861 cctctgagaa aatgcaaacc tagtcagacg aaggagcctt gtctttacta tcatttggga
    40921 caatgcctgt gtccttatta tttcgacgta gatccgaaag tctatacgaa aatcgttgat
    40981 gaagtgaaaa actttttaaa cggaggacac gaaaaaatcg agtcagagat ccatcagaaa
    41041 atggaaaaag ctgctgaaaa catggagttt gaaaaagctg cagaataccg agaccagatc
    41101 cgtgccattg agacaatcat gacttgccaa aaaatgacaa atactgattt actggatcga
    41161 gatgtatttg gttatgcgat tgataaagga tggatgtgtg tccaagtgtt tttcgtaaga
    41221 caaggaaagc taattgaacg agatgtttca atgtttcctt tctataacga agcagaagaa
    41281 gatttcttga cttacctcgg tcagttttat caggaaaatg agcatttcat tcctaaagaa
    41341 gtcttgattc ctgatacgat cgacaagccc agtgtagaag ctttgttagc tacgaaagtg
    41401 ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat tggcgagtaa gaatgcctca
    41461 gttgctttga atgaacggtt tgatctgatt gccagaaaac aggaacggac gatcggagca
    41521 gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa tacgtatcga gtcttttgat
    41581 aactctaata tcatggggac gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa
    41641 ccggcaagaa aagaatatcg gaaatataaa ataaaaacag tcaacggacc ggatgattat
    41701 gcatcaatga gagaagtcat ctatcgcaga tattcgcgag tgattcgcga agggttgcct
    41761 ttacctgatt tgatcttgat cgatggagga aaaggacaag tagatgtggc aaaagatgtc
    41821 ttagaaaatc aattaggaat cgatattcct attgctggca tggtaaaaaa cgataaacat
    41881 aaaacaaatg agttgctatt cggtacagat ttggctgtag taccgcttga gcgaaattca
    41941 ccagagtttt tcttactcca gcgaatccaa gatgaagtcc atcgattcgc aatcactttc
    42001 catcggcagc tacgcagtaa aaacagtttt gcttcccgat tagatgaaat tgaagggtta
    42061 ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca
    42121 gccacgacag aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt
    42181 gataaattac atcaggaatg aggaaagggc actttacaaa gaaagtaaag tgtcctatca
    42241 ttttatgtac aacaaaaaga gcgtttacgt tttgttggta aataataaat cgcttacgtc
    42301 tggtgtctaa ggatatatgt gatttcttga agaaggagtg aagaaatgtt agaagtcaag
    42361 gatcttgtta aaacttttgg gaactacaca gctgtcgatc atgtctcttt ccaaatcccc
    42421 gatggaaaaa tcatggggct aattggccaa aacggggctg ggaagacaac gacttttcgt
    42481 ttgatcttgg attttttgac acaagaccaa ggagaagtgt tatggaatgg ccatcatctt
    42541 ggggagaaag attatgatgt catcggttat cttccggagg aacggggact ttatccgaaa
    42601 gtaacgattc aagaccaact gctttatttt gcagaactta gagggaaatc aagaaaagag
    42661 attgaaccaa aaatcgactt ttggatggaa aaatttcagg taaaagggaa aaagacggat
    42721 aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc cacgttaatc
    42781 catgaaccta aattgatcat tttggatgag ccattcagcg ggttagatcc ggtaaatgct
    42841 gaattgctaa aagatggaat attggaattg aaaaaacatg gttcatgtgt cattttttct
    42901 agccacaata tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga
    42961 aaaatggttt tgaatggaaa agtacatgag attcgtgaat catttggccg tacgaagtta
    43021 ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg
    43081 atccttcatg aagacaatac gttagagatt accttagaag atcctgaagc tgggaaagaa
    43141 atctttactc gtgcgactca gttcggctac attcctatgt ttaaccagca accgccaact
    43201 ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt ggattattac
    43261 caaagatgtc tatttaaaaa atgttaagtc gatttctttc ttgatcatga tattagtccc
    43321 ttttatcatg ctgggtatca tttatgtagc aggaaacttt gctcaacaaa atagtgatgt
    43381 caataaaatc ggcgtattgg ctgataatga gcaaatcagt cagcaaatga gccaagtcaa
    43441 aacagatgat tttgagttca aagcagttga ttctgaagaa aaagcacaaa aagagctttc
    43501 ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag aatgataaga taaatgggat
    43561 gctttatagc gaaaattcat taggacaaag tacacaacta ctgatacaac aacaactgac
    43621 tggcttgcaa tcgatgcttc gagcgagtca attaggcatt tcaccagaag aagtagctag
    43681 cttgagccaa caagcaagtt tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat
    43741 gacagtcgga gaagataact cgacagtcca atacgtcgta agttatgtag caacgattgt
    43801 cttgttcatt attattttga cgtatgcgca gatcattgca caagagatcg cttctgaaaa
    43861 aggcacgcgg atcatggaag tgatcctgtc cagtacgact gcacaaaagc atttttacgg
    43921 aaaattaaca ggtgttttac ttgttgcatt aacgcaaatg gcattatacg gtattatttt
    43981 tggtatagga tacagtcaat tcaaagatat ggaaatggtc aaaaatatat tagacggtat
    44041 ctcattggat aacttgattg gaccattctt gtggtattct cttctattca tgatctttgg
    44101 cattttgatt ttttctgttt tagcagcttt atgtggctct ttggtcaaca aagcagaaga
    44161 tacagcaaaa gcaattttgc cagtgactta tctatctcta ggcggataca tgcttggatt
    44221 gatactagga gcttcagatc caaacaatat cgttattcgg atcacttcgt atattccatt
    44281 cttatcttca tatattatgc cgattcgctt agcaaatgag acagtcggta tgggtggagc
    44341 attgatttcc ttgttggtac ttgtagccgt aacattctta ttgatgatct tgtctgcaaa
    44401 catgtacaaa tcgaatgtac tcgtttatag cgaaggcgga ttatggagtt cattgaaaca
    44461 atcgatttca attatgagga atgaacgaaa aaaagcataa aacaatccta aaatacagaa
    44521 ggaagctgga catctattgt tcccagcttc tttttgtata gacctatttt atttatttta
    44581 ctatattgga tatatattca ttgaaaatag tgtataacaa ctttataata ttgagtatac
    44641 caatttttgg aggcgtttta atggaccgtt atttagattt actttcagaa aaattcccct
    44701 caacggagga tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag
    44761 gaacagagtt atttttgagt gatatacatg gcgaatttcc tgcttttgat catattctgc
    44821 gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttttctgaa caaatgacgg
    44881 aaacggatat cagccaattc actattttta cggcttatcc agaatatgca ttaacgactg
    44941 attggtacaa agaacaagat aaatcgcagc tgatccatca tttgatcgat cttttacggt
    45001 ttacgacagt caaatatacc cggtcaaaag ttcgaaaagg attgccgaaa gaatatagct
    45061 atatcattga ggaactcttg tatatcgatg accgtatcaa tggaaaaaaa gactatgtaa
    45121 aaaaaatcat tgaacaactt gttttgatga aggaagaaga aaaattttta acgaaacttg
    45181 cacagacgat ccaaaaatct gtcatcgatc atttgcatat cgttggagat atttttgacc
    45241 gaggaactca agcagccaaa gtgatggatc gaatcatgga tttttcttct gtagatatcc
    45301 aatggggaaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttac
    45361 tgacactttt acggattgca gcacgctata attatttatt tgaattagag aaagaatatg
    45421 ggttgaattt acgtccattg ttttcttttg ctcatcaaac ttatcagaag aatccagtat
    45481 ttgcgcctaa aaacagtaaa aatctatccg aaagagagca agaagaatta gaaaaaatcc
    45541 atcaggcact ttctatcctt caattcaaat cagaacatca gttattggat cgtcgacctg
    45601 aatttaaaat ggacgatcgt aaattgctgg aaaaaatcga ctatgaagca tcaacgattg
    45661 atttagaagg tcagctctat gagttgaaag gaacttgttt tcaaacgatc cagccagatg
    45721 atccaaaaaa attcttggaa gaggaacaaa aagtgattga tagtttgatg tattcattcc
    45781 agcactcttt gagaatgcaa aaacatatga cgtttttgat cgaaaaaggg ggaatgtatc
    45841 tcacgaacaa tcagcatgtg ctgtttcatg gctgtatacc tgtagacgag aagggacagc
    45901 tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctgggc ttttttgaaa
    45961 aacagataac tgaaagcgca aaagatctga ctgcaaaaga agatttagca accgatttga
    46021 tttggtatgc gtggagtggg ccattttcgc ctttattcgg caagagtaaa atggcgactt
    46081 ttgaacgtta ttttctcaca gagcctcata cgcacgtcga gaaaagcaat ccttattatc
    46141 acttgcgaga tgaagagtgg ttcagtgaaa aaattttagc agaattcgac acaaaagaag
    46201 aagggtcagc aatcattaat gggcacacgc cagtaaaagt aaaaaaaggg gagtcaccga
    46261 tcaaagcaaa tggaaaagta tttgtcattg acggaggttt atccaaagct tatcaaaaaa
    46321 cgacaggaat tgccggttat tcactattat gcaattctta tggtttccaa atcgtgacac
    46381 attatccgtt tttgcctatt gaacaacaat ttgcaaaaaa atccgaccaa acaaatgtga
    46441 aaaaagtaat cgaacagcaa ctaccacgca aactcaatcg tgatacaaca aaaggtatgg
    46501 aactgcagca gcagattacc cagctcaaaa ggctgttgga ctatcgaaag gacgattgag
    46561 aagaattcca gtgaacattg ttccatagac tggagactga gacaaaagtc gtgtcgcagt
    46621 ctctttttcc ggataaacgg tgttcttctg ccaactcctc ggtcacaagt cacactattc
    46681 aacaaacatg aaaagctgtt ttttgaaatt gctccttgtg actcagagtt gaacggcagg
    46741 ctcacgacct cttggtataa acggtgttca aaagccagtt tctcggtatt aagccaaatt
    46801 tatgcaaaaa tgtgaaaagc cattttcaca aaatttttct taatactcga aactaaacag
    46861 ctttttcaca accccctcag aaagtctatt aaaattcatt cacaaagaaa tcgaataagc
    46921 gctgttcaga tggatgtaca cggtgggtaa gttccggatg ccattgaaca ccgaggattt
    46981 tatgcatatc atctttgctt tgaaccgctt caacgagtcc atcgttagaa aaagcgatgg
    47041 cttttaataa aggtgaaaga tctttgactg cctgatgatg gtaagaattc acaaaatatg
    47101 gatcggggag aaggttgctt aacaaactgt ctgaaacaat cttgacttcg tgagtcgtaa
    47161 attgcggggc agtgggctgt tgttcatgtt ttactgacca ctttggatat aatgaaagat
    47221 cttgataaag tgtaccttcc aaaacgacat tcaatagttg gagtccccgg cagactgcaa
    47281 aaattggttt tctttgtttc aatgcttcca agatcagtgc ctgttcaaat tggtctcgtt
    47341 gaatatttgt ttccgttagc tttggatgag gatcttccat gtaaagttgt ggactgacat
    47401 cttgaccacc tgcaagaata agtttgtcga tttgtccaat cagttgagga gctgaatctg
    47461 gagacatgac tggcagaata agaggcaatc cttgagcatc taaaactgca tccacgaatc
    47521 cttgcggtgt atagctcact tgatttcctt ggaaagtatc tgttgctcga atcagttgat
    47581 ttccggcaat gccgatgatt ggtttcattt gtattccccc taaagtagac ttattgtaac
    47641 aaggatttaa aaaaaattaa actatgtaag acttgcccct atacttatct gttcttgctg
    47701 tgttactatc tgtaaagaga aatttcgtgt gaaattcatg acgatagttg aaaatattgc
    47761 ttaatttgtt ataataaaaa gtaagcagaa ataaaaaggt tgggcgtaag cttcaaggat
    47821 ctagttcttc aaggggtgag aaggacattt tattgaaggg gcctgcctat actaagttat
    47881 ctttagaggt acatactttc aatgaaaaaa gaaagcatgt acctcttttt taaatcgaaa
    47941 ggaagtgaag caagtgttag aagctttgga acaaatccga aaagcagaag aagaaaatga
    48001 aaagcgtgct caaacactgc ggcagcagct gaagatctat gcaacagaaa aagagcagga
    48061 attggaaacg atccaaacaa actatcgaca acaactaaaa cagttggtgg aagaaaaaga
    48121 acaatttgaa atacaagaat tagaaaaaga aaaaatgaaa ttgaaagcag aagcagacaa
    48181 ggtgcaagca actttgaaag atcaatatca aaagcatcag agtcaagcga tcggtgcgat
    48241 cattgaaagg gtgaaagaaa cttatggccg tcactaagat ggaaaaagtg accctcattt
    48301 cagataaaag aaatcaggag aggattttac aagcagtaca aggaatccaa aacgttgaaa
    48361 tccgggatct gtatcaaaat agaacaaaca accagtgggt ggagaagtat tttccagata
    48421 ccacgattat tgataaagaa gcaaaattaa gcgctttagg taatcggttg aaagacatcc
    48481 aagaagctat ccaattcata caacaccacg gcgatagccg tcaaaagaaa ctccatttga
    48541 aacgtcaaga actttcctta caagatttgg aagccaatta ttccgaagaa aatttcataa
    48601 ataaattgaa agaagtcctt gatttaaaag aaaagtggga acaactaact gaagaacgtg
    48661 atcgtttaga agaagaggaa gaatggcttc ttcattggca acagctggat attgctccaa
    48721 gtatgtatgc aagtaagacg accatttttg agatggggac tgtgagtgca gcaaattttg
    48781 aaagtttcaa ggaagaactg agtaagcttg atgacgttta tatggaagaa atagattata
    48841 cgccaaaaca agtttatctt tcgtatgttg ctttaaaaaa atcagttgct gttatagaag
    48901 aaattgctgg gaaatacggg ttcaataaag aaacgtatcc ttatgaacaa aatcctaaag
    48961 aaaaactgca agagaccaag cagcaattat ccattgttca cgagcagcaa aagaagataa
    49021 cgacagcgtt aggtcagtgc agtggctata tccaagaatt tgaatgggta gaagaagtca
    49081 cacttgccgt tgcagaacga gaaaaaataa aagaacggtt tatccatgcg acttatctga
    49141 tcgttttgca aggttgggta gatgcagaag aaaaacagaa tttgctgcat gtgttaaacg
    49201 aaaccgtatc agaagaagat atatacgtca gtttcgaaac accaaactca gaagagattc
    49261 agcaggaagt ccctactaaa ttgaaaaacc atccgcttgt cgaacctttt gaattgctga
    49321 ctgaaatgta cagtctgcca aaatatgaag aagtagatcc aacaccttgg atgacaccat
    49381 tttatcttgt cttcttcggg atgatggtcg cagatgttgg ttatggtttg ctgatgctca
    49441 ttgggacaat actagctcaa aaactacttg ttctaccacg cgggatgaaa cgtttcgtaa
    49501 aattctttga gattctctcg ataccatcga tcatctgggg attgatctac agttcatttt
    49561 tcggacagtc attaccaaaa gaactgtttg gcatccgctt gcctttccca atcctatcta
    49621 cgacggatga cgtcaatacg attttgattt tatccgtgat tttcggtctg atacagattt
    49681 tggttggatt atttgtggca gcaaaggaaa atatcaggag aaaagattac ttgagtgctg
    49741 tcagtgatgg atttgcctgg caagggatcc taattgggat cgttattgcg ttggttggtg
    49801 ccatgttgat aaaaaataca gcatttgttt accttggcgc tgggctatcg atcattgctg
    49861 ccttatgtat cttgatcgtt ccgattatcc aaacgccgtc taaagtcaaa ggtgctgcca
    49921 aaggtgcgta taatctttac ggactgaccg gctatatcgg tgatttagtc agttatacac
    49981 gtttgatggc attgggtatt tcaggcggaa gtattggagc ggcattcaat atgctagttg
    50041 ctttcatgcc gccagctgct cgattcagtg tagggatcct tttgatcatc gctttacacg
    50101 cactgaatat gtttttgaca ctgctaagtg cctatgtcca tggcgcacga ttgcagtatg
    50161 tcgaattttt tgggaagttt tatacaggag gcggacgtgc ctttgatccg ctgaaaacgg
    50221 cggaaaaata cgtcaacatc aatcacaaga aaaaaaacaa taaataattg gaggattttt
    50281 atataatgat ggattactta attagtcaaa atggtggaat ggtatttgcg gtattagcga
    50341 tggcaacagc gacgattttt tcagggatcg gttcagctaa aggtgttgga atgacaggtg
    50401 aggcagcagc agcattgaca acaagtcaac ccgaaaaatt cgggcaggct ctgattctgc
    50461 aattgcttcc aggcacgcaa ggtttgtatg gattcgttat tgcctttttg atttttatca
    50521 acttaggaaa tgacatgtca gttgtacaag gactgaactt tctaggtgct tctctgccga
    50581 ttgcttttac aggcttgttt tcaggtattg cccaaggaaa agtcgcagcg gcaggtattc
    50641 aaattttagc gaaaaaacct gaacatgcta caaaaggaat catttttgct gcgatggttg
    50701 aaacatacgc gattttaggt ttcgttattt ccttcctatt agtgttgaat gcctaagcat
    50761 aaaggaggaa aagcagatga acgccattga aaaaatcatc tctcagatga acgaagcagc
    50821 agagcaggaa agagcggcgt tagaacaaga agagcgaatg aagatcgatc aaaactttga
    50881 acaaaaacgt acgcaagttg aaacagaaca tcaaaaacaa aaagaaaaac aaatcgaatt
    50941 actcgaaaaa aaataccgtc agctaaggaa tcgccagcaa gtagaagtac gacaagaaaa
    51001 tttgaatgcg aagcaggaat ttttaagacg attgtttgca gatgcggtaa ctgaaatgga
    51061 aaattgggat gaatctgaac aaatccagtt tatcaaaaat gcattgtatt cattaccttt
    51121 gacaggaaaa gtagcattca ttgctggaga aaaatcagca gcctatcttt ctcagacgtt
    51181 gttggatgaa tggaacaatg aacttccctt tatgatggtt ttaagtgatg aaactgttgc
    51241 tgatcaagcg ggttttctga taaacgatca aggagttcag tataattttc ttttcagcag
    51301 ccttgtacaa gatatccaag gaacgatgag ttttgagatt gccaatcaat tattcgaata
    51361 aggagggatc agtgtatgga ataccacgaa ctaaatccct tgattcgcgg aagagagctt
    51421 gaacttttgt ccaatgagac atttgaacgg atgattcaaa caaattctgt cgaagcactt
    51481 ggtgaaatat tgaaatccac gatttacagt ccatatattt atgacggata tgaatatgac
    51541 ttcgaacaga atcttttaaa ggagcagagc cgtctatttc gctggttaaa ggaatcagca
    51601 ccggagcctg aaatcgtttg gatctatacg atgcgttata ctttccataa tctgaaggtt
    51661 ttgacaaaag ctgagatgac aaatcaaaat ctggaccatt tgtatatcga cgatggattt
    51721 tattcattag aaaccttgaa ggatgccatt catacgcaag tttcaaatga acttcctgaa
    51781 agcattatgg acagtatacg agaagtacgt gattattttg aagagtctac cattcttcag
    51841 ggaattgacg tgatttatga tcgacagttt ttgacagagc agcgacggct tgccgataag
    51901 ctaggatatc aagaattgct gacagaagtc attgctttta ttgacttgac caatatcaca
    51961 acgatggctc gaggaatatt gcagaaaaga acatatgggt tcatgacgac ggtactttcc
    52021 agctcgggaa gtatcgaaaa agaagtattt ttgaactttg tagataaaga tatggaagat
    52081 tacacgaatt ttctattaga gacagattat gctgattttc tgcgccctgt catccgtgag
    52141 ggaaatattg atctcgtaaa gcttgaacag ttgaaagatg attatctaag ttcattgtat
    52201 caagaagcac aaacccaggc tttcggacca ctgccactac tagccttttt gaacgcaaaa
    52261 gaggtagaaa gcaaaaacct tcgtttactg gttatcggca aaagaagcca tttcgaatca
    52321 gaaacaatca gaaagcgggt gagacaagtc tatgacgcat aagatcggtg tagttggcga
    52381 caaagattct gttttgccat tcaaattgtt tggctttgat gttcgttatg gtacaaccaa
    52441 gcaaaccgtt cgtcaggcaa ttgaagaaat ggcaaagaat gagtatggcg tgatttatgt
    52501 cacagagcaa tgtgcagcat tgagcccaga aacgatcgaa cgttacaagg aacaaatgat
    52561 acctgctatc gtattgattc ccaatcatca aggatcatta gggatagggc tggaagaaat
    52621 tcaaaaaaac gtagaaaaag cagtaggtca aaatatctta tagtatgaaa aggagataga
    52681 aattttgcaa attggaaaaa tcataaaagt ctccggacct cttgtaatgg ctgagaacat
    52741 gtctgatgcc agtatccaag acatgtgtct agtgggagat ctaggagtta tcggagaaat
    52801 tatcgagatg cgtggcgatg tggcgtctat tcaagtatat gaagagactt ctggaattgg
    52861 tccaggcgaa ccggttcgct ctactggtga agctttatct gttgaattag gaccagggat
    52921 catttcacaa atgtttgacg gaatccaacg gccattagat acctttatgg aaatcacaca
    52981 aagtaatttc ttagggcgcg gagttcaatt gcccgcattg gaccatgaga aaaaatggtg
    53041 gttcgaacca acagtagaag ccggagaaac ggtgagtgct ggagatgtta tcgggatcgt
    53101 agaagaaaca aaagttataa aacataaaat catggtgcca aatgggataa aaggaacgat
    53161 caaaacaatc gaaaaaggtt cattcactat tgatgaaaca gtatgtgtca ttgaaaccga
    53221 aaatggtgac aaaaatttga cgatgcttca aaaatggcca gtaagacgta gtcggccaat
    53281 caaagaaaaa ttaaacccgg atgcacccat gatcactgga cagcgagtga ttgatacatt
    53341 cttcccagtg acaaaaggag gagcagcagc tgtacctggt ccttttggtg cagggaaaac
    53401 agtcgttcag catcaaattg caaaatgggc cgatgtggac atggttgtct atgtgggatg
    53461 tggcgagcgt ggaaatgaga tgacggatgt tttaaatgaa ttccctgaat tgattgaccc
    53521 taatacagga gaatcgttga tggaacgtac ggtattgatt gctaatacat caaacatgcc
    53581 agtagcagct cgagaagctt ctatctatac gggaatcaca attgcggaat atttccgtga
    53641 catgggttat gacgttgcta tcatggcaga ctccacttca cgatgggcag aagcacttcg
    53701 tgagatgagc ggacgtttgg aagaaatgcc tggtgatgaa ggttatccag cttatcttgg
    53761 ttcacggctt gcggaatact atgaacgatc tggaagagtc attgcattag gatcagaagg
    53821 tcgcgaagga agtataaccg caatcagtgc tgtatccccg tctggagggg atatttcgga
    53881 accagtcaca caaaatacat tgcgggtcgt aaaagtattc tggggtcttg attccagttt
    53941 agcccaaaaa agacattttc cttcgatcga ttggatacaa agctattctc tttattcaac
    54001 agaagtaggg aagtatatgg atcatatcct tcaagctgac tgggcatcca tggtcaccga
    54061 gggcatgcgt attttgcagg aagaacagca gctgaatgaa atcgtgcgtt tggtagggat
    54121 tgattccctt tcagataatg accgtctgac attagaagtg gcaaaatcca ttcgtgaaga
    54181 ttatttgcag caaaatgctt ttgatgatgt cgatacattc acttcaagag agaaacaatt
    54241 caaaatgttg aagatgatct tgacctttgg tgaagaagga cgccgtgcgt tgtcgttagg
    54301 tgcttattta agtgagatca tggacggtac cgtatcgatt cgtgaaagga tcggacgaag
    54361 caaatatatc ccggaaacag aattagaaaa actagatcaa attattggtg aaatcaagca
    54421 aacgatgcaa acgatcgttt cagaaggagg catgagcaat gattaaagaa tatcgtacga
    54481 tcggtgaagt cgtcggtcca ttgatggcgg ttgaaaaagt tgctggtgtc aaatatgaag
    54541 aattaatcga agtccgtatg caaaatggcg agattcgtcg tggacaagta ttggaagtcc
    54601 aagaagataa agcaatggtc caaatattcg aaggaacaag tggtatcaat ttacgtgact
    54661 cttctgtacg atttttaggc catcctttag aactaggtgt atcggaagac atgatcggtc
    54721 gagtattcga tggtttggga cgaccgaaag ataacggacc agagatcctg cctgaaaagc
    54781 atttggatat taacggagaa gtaatcaatc cagtagctcg ggattatcca gatgagttta
    54841 tccaaacagg tatctctgca atcgatcatt tgaatacatt agttcgtggg caaaaattac
    54901 cagtattttc tggctctggt cttcctcata aagaattagc tgctcagatc gcgcgacaag
    54961 caacggtact cgattcatca gatgatttcg ccgtagtctt tgctgctatt ggaatcactt
    55021 ttgaagaagc tgaatttttc atggaagatt ttcgtcaaac gggcgcaatc gaccgttctg
    55081 tcatgtttat gaatttagca aacgaccctg caatcgaacg tatcgctact cctcgtatgg
    55141 ctttgactgc tgcagaatat cttgcttatg aaaagggaat gcatgttttg gttatcatga
    55201 cagacatgac caactatgca gaggcattgc gagagatttc tgcagcacgg cgagaagttc
    55261 caggaagaag aggatatcct gggtatcttt acacgaactt agctacttta ttcgaacgag
    55321 caggaagaat ccgcggacta aaaggatcgg ttactcaaat tccgattcta acaatgccag
    55381 aagacgataa aactcatcca atccctgact tgactggtta tattacagaa ggacagatca
    55441 tcttgactag agagctttac aagagtggga ttcagccacc aattgatgta ctcccgtctc
    55501 tttcgcggtt aaaagataag ggaactggac ccggaaagac aagagaagac catgcagcta
    55561 cgatgaatca gctttttgca gcctatgcac aaggaaaaca ggcaaaagaa ttagccgtag
    55621 ttttaggtga atctgcttta tctgatgtag acaagatcta tgcgaagttt gctgagcggt
    55681 ttgaaaaaga atatgtcaat caaggattct acacgaaccg ttcgattact gaaacacttg
    55741 atctagggtg ggaactgcta tcgatgttgc caagaaccga attaaaacga atcaaagacg
    55801 acatgctgga caaatatctg ccagaaggga agtgagttct gatggcacga ttgaatgtga
    55861 atccgacccg catggaattg actcgattga aaaaacagtt gacaactgcc acaagagggc
    55921 ataaattatt aaaagataag caagatgaac tgatgcgtca attcatcctg ttgattcgaa
    55981 agaacaacca gctgcgtcaa gaaatggaag agtctatgac agaagcaatg tctgattttg
    56041 tattggcaaa tgcttcgatc acggaacctt tcatcgaaga attatttgtt ttgccggcgg
    56101 aaaacgttga tttgtctatc gtagacaaaa atatcatgag tgtaaaagta cctgtgatga
    56161 attttgaata tgatgaacag ctgacagaca cacctttgga atacgggtat ttgaattcca
    56221 gtgccgaact ggatcggtca atcgatcggt ttactaccct gcttccaaaa cttttggaac
    56281 tgacagaaat cgaaaaaact tgtcaactga tggcgagtga aatcgaaaag acacgtcggc
    56341 gagtcaatgc attagaatat atgacgatcc ctcaattaga agaaacgatc tattatattc
    56401 gtatgaagct ggaagaaaac gagcgcgcag aagtaacaag gttgatcaaa gtaaaaaata
    56461 tgggtaccac tgactaagtg gtatcttttt ttaaaagagg aaaacgatga ttaaacaaag
    56521 caaacaaata aagaaacgtt tgccggctgt tcagcttctc gcattaggat tttttctcct
    56581 gattttagtt ggcggcggcc tacttaccct accatttttt agtaatagtg ggcatgggac
    56641 aaattttatc gatgcgcttt tcactgcaac ttctgctgtc tgtgtaacgg gattgacaac
    56701 attgaataca gcagagcatt ggaacagtgc tggtcaattt ttgatcatgc ttttgattga
    56761 gatcggcggt cttggtttta tgatggttcc gattctatgc tttgcattga taaagaagaa
    56821 agtcagtctc agcacacgta ttatattaaa agaagcgctg aatctagaag aagtatcagg
    56881 tgtgatgaag ctcatgcttt acatcctaaa attggccttt gtgatccaat tgattggtac
    56941 ggttgcttta tcttttgtat ttgtgcctga atttggctgg ttgaaaggta tctggtacag
    57001 tttgttccat gcaatctcta gtttttgtaa tgcagggttt gatttgttag gagacagttt
    57061 agccaatcac cagcaaaatg tttatttgat catggtggta tctgctttga tcatttctgg
    57121 tgggttaggc ttcatcgtat ggcgtgattt actggaatat catcagaaaa agaagatgac
    57181 gattcattca aaaatcgcat taaccgttac aggtaccctt ttattaggcg gattcgttgt
    57241 gttctttttc acagagcgca atggggcaac gctagtggaa gggaatatgt ttcaacgatt
    57301 ggcaaacacc ttttttatga gtgtgacacc tagaacggct ggatattatt cagttgatta
    57361 cctccatatg agccatgcag gtctgatttt aacgatgttt ttgatgtaca tcggcggaac
    57421 atctgggtcg acggccggcg gattgaaaac aactacgtta ggtgtgttgc tgatccagat
    57481 gcgttcgatg ttgaaaggac gtacgagagc ggaagcattt ggccgtacca tccgacaagg
    57541 cgcagtgttg cgggcactta ctttattttt tgttacgttg acgttgtgtg tagtcgccat
    57601 catgatttta tcggtgactg aaacgatacc taaaacttct ggcatcgaat atattgcttt
    57661 tgaggtcttc tctgcgtttg gtacagtagg tcttacgatg ggattgacgc cagacttgac
    57721 tgcaataggg aaattgatca ttatagcgct tatgtacatt ggacgagttg gtgtattgac
    57781 tgtggtcttc tctctgatga caaaagcaca aagaacacaa tcaaaataca aatatccaga
    57841 agaaagtgtc atgattggat aatttcatct tctgacagaa acctagtgat ttagtaaaat
    57901 aagaaaataa aataattttt cttgcttctt ggatacttta agtgcaaaat gttcgtttat
    57961 tttttgaggc actacccgtt gttgaacctc gggtagtgcc tcttttttat tgaaaggaat
    58021 caataagatt tgattccttt atttatcaat aggttataat tcatattttt ttattttttc
    58081 ttttacgata cttagtccag aagctaaaat ttcaaggtta ttagaggata gggaaattcg
    58141 aacgaatgca tctggttgcg cacgtgtagt aaagcgtctg gaatgataaa gtcggatacc
    58201 gttagcaaga aagtcattct caattatctt agggtcaatc tcaggatcaa ctggaagaat
    58261 tcgatagtaa gggacacgat tcgttggttt ttctactttg aaaagagaat caaaaagctg
    58321 attggctctt tttgtaagac ttaatttagt ttctaagatc ctatttgctt catttgaatg
    58381 gaggatttga gtgatgactt ccacatctaa tccagaagtt ttcacattta tattaaaaat
    58441 tgcttgttct atcttgcttt ggacatgtac tggaactaca agataagcaa ctcgcaatcc
    58501 agaacaaata aattttgtca ttccagctaa gtagattgtt tggttaggta ataaattttg
    58561 gaaaggagga atgatcgtat gttgatagta agttgttaaa aatgaatgga tatcatcttc
    58621 aataacccat agaccttctt tttcaatcac ttcggctaat tgctctcgtc ttttgtgaga
    58681 gatttgaaac cctataggat tattacaaga aggcataaga aagacaccat gaatcttctt
    58741 ttttctacat tcctgaatca atatatcagg tcgcattcct tgctcgtcaa aatcaattgg
    58801 aacgatctct aaatgatata actgagccaa ttcaatgaaa ttagcgtatg tatatcggtc
    58861 tgttgctatt ctatcacctg gagagaaaag tgcagaaagt gtgatagcca atccattttg
    58921 aacaccggaa acaatagata gagattctaa ggacggaatg actccttgag tttttaacca
    58981 atttgctgca gtagtcaatt gaaaatttgt gcctttggga tcttggtaat taagcaaacc
    59041 aacaagattt gggttattac tagttgattg aataaacggg acaacgaatt tattacattc
    59101 ttcaaaagaa ctaaccagac caaaatcaat gatatgttcg gttacttcat tggtagagat
    59161 ggtgatggac tcaaagacat tgggagaaat aaatgtgcca cttccaatat tcgtatacag
    59221 caaacctttc tcaattccat atttataagc cttgccaacg gtagtaaaat ttaaatctaa
    59281 ataatcagct agctctcttt gggagggtaa ctgagtgttt ttctgtaatt tattctttaa
    59341 aatatcttgt tctaattgtt gaatcaacga tcgataataa ggacgcttca atttttgttt
    59401 gtctggtcgc caagttaacg ggtaattgtc ataagaatta ataggcattt tctccactcc
    59461 ttaaaattgt ataccataca attttaccat tgattgtatt gattttagaa gttaatcttt
    59521 tgtgaggagg gaattgtcta tgaaaattat taattttgag gaagtagtag cttcttttag
    59581 ttttgaagaa gcgattcaag taatgaaaaa atgttttttt gattatgaaa gtggtaaaat
    59641 cagccaaaat cctcgattgg tggaaatttt acctgacgga aaaggcaaaa atatgtttgc
    59701 gatgatgcct gcttatttgg gggagaacag atattttggc acaaaagtca ttaccgcttt
    59761 tccggataat catcagtatc atttaccttc tcatttagga gagattttct tgtttgatgc
    59821 tcaaaacgga ctacccaaag cattgataaa cgcaaatgca gtcacctgga ttcgaacagc
    59881 tgctgtatct gcgctggcaa cagattactt agcaaatccg catgcagaaa cacttgcttt
    59941 aattggagct ggtcaacaag cattgtcgca tttgactgcg atttctacca ttcgtcccat
    60001 cactacagtg tttgtttacg atattttaga agaaagacga aatactttta tcgctaaagc
    60061 taaaaaacag tatcctaaaa taacgtttat aaatgcaata tcagtagaag atgcagtacg
    60121 tccagcggaa atcatttgta cgttgacctc tagtaaagag gcatttcttc aaaaagagtg
    60181 ggtaagggaa gacgcacata tcaatgctgt gggcacattt actcctgata caagagagat
    60241 aagtacatcg ttgatggaaa cttctcaaat ctatgtagat gattactcag ctgccttaac
    60301 agaaagcggg gacctgctta tagccatggc agaaggagat ttagaggaat cttgtattgt
    60361 tggtacatta ggcgaattag tttctgagaa aaaaacattt tataaaggag aatcgaggcc
    60421 gacgattttc gacgcagttg gtctagctat tgaagatctt tgttgtgcag aatatattta
    60481 taacaaatta gagaaagggg ctttaaaatg aacttagatt attacgtagt tgatgcattt
    60541 gcagatgagg tgttcaaagg aaatccagca gcggtttatg tgctagaaga atggctgcca
    60601 gaaggtacga tgcaaaaaat tgccattgaa aacaatttat ctgaaacagc atttacagtg
    60661 aagaaaaacc aagaatttga gcttagatgg tttacgccag atcgagaaat cgatctttgt
    60721 ggtcacgcga cattagcaac agcatttgtt ttatttaact attacaaaat acctgatgaa
    60781 acgattaaat tttctactca aagcggtaat ctatttgtga caagaattaa agattattat
    60841 tacatggatt ttcctagtat tatgccgaaa aaagtgccga tattgtctga atatgaagaa
    60901 gcgattgggg ctaagattaa agaagcctac ttagcacgtg atttattttt cgtattagaa
    60961 gatgaaaaaa cggtggctaa attgcagcca gattttacag ctatcaaaaa ttttgaatta
    61021 ggaataggag tcatcgtgac agcagaaagt gtgcaaaaag actttgtttc tagaaccttc
    61081 tttccaaaat taacaatcaa tgaagatcca gtatgtggat cagcacattc taatcttatt
    61141 ccttattggg cagaaagaat gaataaaaca aaattgtcag cttatcaaat gtcatcaagg
    61201 ggcggggatc tagaatgtga attaaaagaa gatcgtgtga taataggagg aaaagcagtt
    61261 ttgttttcta aaggtacctc gtttatttga tttctttcaa atgaattaaa aataaattgg
    61321 tggagagtgg caaccttata gctacaatgg ttgttaaaaa aagacgcaaa ctttttcaat
    61381 ttaatgaaaa gtttgcgtct cattttaatt attggtgttt gttttttatt caatcaagag
    61441 taaaatgccc catactaact atcctcggat gacttcgatt ttataaccat cagggtcaat
    61501 gacaaagtag taagaaggcg cagtgccagg taatcctttt aagtcagtaa tctcaagacc
    61561 agcagcttta tgttgctcgt gtaatttttc tagatcatca gctgctatag cgatatgacc
    61621 atagccgtct cctagatcat acgcaccatg atcatagttg taagtcaact ctaattcata
    61681 gtcatcccca ggaagtgtca aataaacaag tgtaaatttg ttttctggaa aatcacggcg
    61741 gcggctttct tcaaaaccaa atgccttttg ataaaattcg attgatgctt caagatcttt
    61801 cactcgaaca catgtgtgag ccattttcat agattcatct ctccttttcc ttttactcaa
    61861 tcataacaaa aagaagtaag gaaatagttg atttgtgctt gttcatgaga aatttctcat
    61921 gtaaaatgga tagaaaataa aactgttaag gtggaaacta ttcatggata aaccaatttt
    61981 tggtgaaaaa aagcaatttg ctgattatca aacccgttat gcagcatata ttgtcataga
    62041 aagagatcag cagattgcag taatcgaggc tccaaatggt gctttttttc ttcctggcgg
    62101 ggaaattgaa ggagaagaga cgaaagaaga agcgattgtc cgtgagctga tagaagaaat
    62161 gggaattgtg gcagaaattt cttcttatct gggagaagct gatgagtatt tttactcgaa
    62221 ttatcgtgca acttattatt ataatccagg atatttcttt gttgctgatt cttggaagcg
    62281 tataggagaa ccaactgaaa agaccaataa aatttggtgg gtgagccctc aagaagcgct
    62341 tgtgaaattg aaacgtggta gccaccgatg ggcagtcgag agatggatcg aaaaaaataa
    62401 aaactgaaag aaagcgagta gatcagcttt ctctcagttt ttttatttat tacagtacat
    62461 attttttgat tgcttcagct acaccgtgct cgtcgtttga tgaagtgatg acatttgctg
    62521 cttctttgac aagagaaacg gcatttccca ttgctactcc tagaccagca tattcaatca
    62581 tggataagtc gttctcgtta tctccgatag ccatgatttc atcttgatca ataccaaggt
    62641 gctgtgcgag attcgcaact gccgcacctt tacttgcttc tttatttagt acttcgaagt
    62701 aatagggcgc acttttgacc gttgtgtatt tatcacggaa agtttgcggt aaacgcgcaa
    62761 ttgctgcatc taaaatttct ggttcgtcga tcatcatcat tttgacgatg ttcatgtctg
    62821 gtgtcatttc ttcaggtgtt cgatatttta gcggcatctt gactaaactt gcttcgtgga
    62881 tcgtataagc gctgatatct cgattaggcg tgtagatcgc tttgtcatcg atcgaatgga
    62941 gatgactgcc gacacggcgt gccattactt cgatctctag gaaatcttca tgtgtcagtc
    63001 cgtgacgggc gatgatttta cctgatttcg tttgttgtac gagtgccccg ttataagtaa
    63061 taacgtaatc attatcttga aaaaggttca actcgtttaa ctgttcctgg acacctggta
    63121 agggacggcc agtgcataaa acgatcgaaa caccttttgc aacagcagcg gcaatcgctt
    63181 cttttacttc ttttgttact tctcttttct catttaataa agtaccatcg atgtcgatag
    63241 cgactagttt gatagacata atttcctcca atatatttta atttatttca atcaatgcgc
    63301 cattacggat atggctggca aattcttgat aagtttcgtc aaataaatca taatgatcac
    63361 gcaaggacgg atcaaccatt tcttttggaa agaaaaatcg ctcatctcct tgttcttgtc
    63421 ccgctaatgc agcaacaagc tgactaacct gtgataattc tatcaaagtt ccgtcatttc
    63481 gcaaaatctc gatttgtgtg cgatgtctgc cttgttctgg gcgataaaaa tcataaggta
    63541 gatcgtagct agaattcaca gcagtgtaat aaacaggatt atatccgacc ttttctacta
    63601 acaaagtgag ttcttggact aattttgaat cccggttgcc agaaaaagta gcagatttca
    63661 gtggtttacg attcaaaaaa cgtttagcta aatcatttaa aataggatca gcagattcag
    63721 accattgagt aaagtaagtg cccaagaccc catcatcaag tttgaggtat tcttctaaag
    63781 taaagcttcc gtcaagaaat ggcagaagta attgagaatg gagttcaaaa ctgctggcat
    63841 cttcttgata caactcgtgt gcgcgatgaa gcagatgctc caaaattact tccataccac
    63901 gggaaacagg atggaagtac acttgcacat acatttgata tcggctaacg atataatctt
    63961 ccactgcatg catgccactc atggaaaaag cgatcccgcc ttcataagga cgaatcacac
    64021 gtaatattcg ggtaagatca aatgttccat attctgttcc agtgaaataa gcatcccgaa
    64081 gcaaatagtc cattcgatct gcatcgatct gactagaaat catttggaca acttgtgggt
    64141 tggggtaagt tttttggatc acactagcta ccttttcagg aaaaccttct tctactcggt
    64201 taagaatctg atacacttct gtttctggag aagtgatgat ctctacagtg atggcttcat
    64261 gattcgtatg gaagatatgt tcaaatgtat gagagtaagg accatgaccg acatcatgca
    64321 acagtgccgc acataaagtc accagccgtt cctgatcatc ccagcctccg ttgcctattt
    64381 tttcttttga aaaatttcga gaaaagatat cgcagattcg tcttgaaatc tcataaacgc
    64441 ctaatgaatg agtaaaacgg ctatgttctg ctccgtgaaa agtgaaagaa gaagtgccta
    64501 attgtttaat gcgtcgtaga cgttggactt ctttcgaatt gatcaaatct aagattactt
    64561 gatgctggac atggatatag ttatggactg gatcacgaaa aactttttcc ataggcagca
    64621 tttgatattt ataaggggtt gtcataatgt agctccttta tgttattacg ttgttccatt
    64681 ctctggattt ggttgttcca ttgctccagc tggatttctt tttttagcca gtcggtagaa
    64741 tcctgtaaat cacttgcaga atacagcttt tcaaaagctt ttgcaaatgc ttctttgact
    64801 tcttttacaa aaaggggttg gtcaagcagt tcttcaagtg ttgccattgt agcaggatcc
    64861 actgcaggat agccatttgt accaaagtct tcttttaaag cagattgata aaatcgcttc
    64921 atgacttgcc cacgtttttc ctgattaccg ttgacactta agtacatcat aacagaaatc
    64981 ccatttttga tccgacgctg agcgataccg gcaaattttt tgccatggat gcttaaatca
    65041 taagttccag gacaataaga atgggtgatt tcgaatgctt gtatcggtgc ctttggaaaa
    65101 gcgtatttag tcaataggag catttgctcg taagcgtcat ctatggttat tttttttgtg
    65161 tcactttggg ggaaaatcaa agaaatattc aaaattcctt catctgatac gatccctaat
    65221 ccacctgaat ttctgatcac tggagcgtaa cctgcatctt ccagtgtttg gatcccgtca
    65281 cttagaaaag gagttcgaac atccttcata cctaagatga ctgtgcagtc atattgccag
    65341 aaatgtaaaa agatttgctg gtgttcttta gaatattccg ttaatatatc tgttaatgca
    65401 aaaggggaaa agtcgttttt ttgataaatg tgctgatcta gtcgaatcgc agggttcatt
    65461 caaccggctc ctttgtataa attcatattt tctacaatat tatactctaa aatcaaagag
    65521 ttgttttgga aaaaaggttt tctgtaaaaa tggacaagtt ggttgacaaa acaaaagaaa
    65581 ctaggaacaa tgtagctatg agaagtaaag gagaatgaat aatggaatta tcacaaggaa
    65641 caccgatttc aatcaaattg cgtacaaaag taaaacagca aggggaagtg caagattttt
    65701 attttgattt gaaagggcaa atggtgacaa ttggcgatac gctgtacata cgttataaag
    65761 agattcagga ggagacgggc gatgagattc ctgtaacgat caaattagtt ccagatggtc
    65821 atgtacagct gatccgtgca ggagaaatgc ggatgcgttt gaaatttggc tataaagaac
    65881 ggttagaaac atcctatcga acaccatacg gtatgatcca gattgctact tttacgaaag
    65941 aactgcatgt tagtttgaaa gaccgtccca cagcagggaa ggttcgaatc gattatgatc
    66001 tgttcatggg accggaaaaa atcggcgaat attatttgac cttggatttt acagcgtaaa
    66061 tataaaagaa aagtttgatt ttttgtgtct gattttgtat gataaggagt agactgtgaa
    66121 aggacgtgtc ctgattggaa attagtgtat ttgaaggtgc aaacaaaagc gaattatcaa
    66181 tgatcgaagt agcacacgcg atcttagaac aacgtggcga tgtcatggat ttttctgatt
    66241 tagctaacca gatccaaaac tatcttggga aatcagacag tgaaattcgt gattcactcg
    66301 cacaatttta tactgacttg aatattgacg gcagctttat ctcattaggg gataatcgtt
    66361 ggggcttgcg ttcatggtat gctatcgatt cgatcgatga agaagtaaac catggaatgg
    66421 acgaagagga tgaagatact ccacgtcgcc gtaaacgcaa gaaagtcaac gcctttatca
    66481 atgatgatga agacgcgatc gattacaatg acgatgaccc agaagataca gatctgacag
    66541 aagaagatga cgatgatctg tttgatgacg atgatgatga agatgaagaa atcgctgctt
    66601 ataattctga cttgcaagaa atcggagcag atgacccgac agatgatgaa gaagacttgc
    66661 caggaaatat cgaagaagat ttaagtatca tagacgaaga cgatgatgat gaagattatg
    66721 aagaggaaga attttctgac tctgaaaaag aataaacatt attttcttac gagaggttgt
    66781 gacagagacg atcagcctat cagaataagg aggtgtgaca cgatttttgt cacaccttct
    66841 tttttgtata taaaaatccc aagtaaatac ataaaaaata aaaaaattga aacttttagc
    66901 gcaccttgta cgtcatatta agtgaggcag aaaggagtgg agaatgattt ttactggata
    66961 taagaaaaaa cggcagatcg aaaaataccg tcacctgctg aatggactca tcgaaactga
    67021 ttatcaaaaa atgttccgta tcacgatgaa ttacgtacat gataaagaag aggccttaga
    67081 tgtgatgcaa gatagttttc ataaagccct gaactatttt gaaaaagaaa aagagatcga
    67141 gcattttagt gcatggtttt atcggatatt gatccgcacg gcattagata gttggcgcag
    67201 acaaaaaagg acgcctgtgg acttatttga aattcaagat atcccccaac agttaccttt
    67261 agaaacttcc gttgcagagc tccatatgat tttagataaa atagacagtc cggaaaaaga
    67321 gattctgatc ctctactttt ttgaaggatt tcgtttgaaa gaaattgcga ctatcttgga
    67381 tatgaatgaa aacacagtta aaacgaaaat gtaccgctca ttgaatgctt tacgtcaaat
    67441 cctagaatga aggagagaag agtgccatgt attcccaaaa agaaaattta aaaaaactaa
    67501 agaaaactta taaagaacaa gcaattccca aagggcttaa agaggagatc cgccagcgtt
    67561 ttattatcga agagcaatca tttataaaag caaagcggag aaagagacgt ctccaagggt
    67621 tcggagtatc tttggctatc gtaatgatta caacaagtag ccttcttttt aatagtcaag
    67681 ttcgaagctt tgcagaagat ttacccatct taggatcagt cattgaattg atcttaggcg
    67741 aacgctttac tgatcgatct gaaaaaatcg atattcaggt accgaagatc aatacacaaa
    67801 atgagaaaga aaataaaaca attcatggat taaatcaaaa atactttcgc gaaggacaag
    67861 cagattttga aaaagctgaa aaagaatatg gaaattttga aacggatcat taccaagttt
    67921 tgggagatta tcagaaaatc gtagacgata accggtttct cgtgattgaa aggcagatca
    67981 cccaaacagc agcagatagc catgttgaaa aaagatacga cacaatcgac aagaaaaaca
    68041 gtgtccagct ctcacttcca cttttattca aagatgacac ctatctatct gttttgacaa
    68101 aagaagtcaa acggcagatg gccgaacaag tcaaggaaga tccatctaag tactattgga
    68161 ctgagcaaga tattcaagaa gggacgatag aaaaaccaac acttgttacg cctacacgaa
    68221 gtttttattt gaacaaagag catcaattag ttttgacttt ttctcaatac gaaattgctc
    68281 ctggttatat gggtacacca gaatttgtca ttcctaagtc cgtgacaaaa acgattttgg
    68341 catcggaaga ttatttggat cattagacga gaagtagttg cgactagcag tttcaatttt
    68401 caagtaagta caagtaaata ataagaagag aaaaagagaa agaagagata tagtgaaaag
    68461 aaattattta gttatcagta ttattgcttt atccgtgctt ttgtttttcg aaattacttc
    68521 ggtggcctcc ttgatcaccg aagtaaaagg acaaacacac tcttcggagc attcgtcaca
    68581 atctttcaaa caatcagaac aatcaaccat taaaagccaa tcaactgaaa cagctaacgt
    68641 caaagaagcc attcaagaat cagcggaaaa ggtacaaagt cagtcatcac aatcaacaca
    68701 agacagtaca aaaacgagcc gagatccggc tgtcaaaagc gtcgcaatca gctttgatga
    68761 tggaccagga gcgaccacaa caccgcagct tctgcgaatc ttaaaagaaa aaaacgtgca
    68821 tgccactttt ttcgtcttag gagaaaatac ggcgcagcat cctgaaatcg tgaagcagac
    68881 agctgaagca gggcacgaga ttggcaatca cacatacgat catcagaatc tggccactct
    68941 ttctgcccag tcgatgacgg aggaagtgac gaaagcagac actgagatca aaaaagtcac
    69001 tggcaaaacc cccacttttg ttcgtccgcc ctatggttct gtcacgaatg taggagcaac
    69061 gatcatccag cgaccgatca tcgaatggtc tgttgattca gaagattgga agacaagaaa
    69121 tcctgatttg attttgcaga agatccaagc aacagtatac gatggggcaa ttattttatt
    69181 ccatgatatc tatcctgaaa cgattcgtgc cgttcctcaa gtgatcgatt atctgaaaga
    69241 gcaagggtat cgcatcacga cagttgggga tttattaggg catccaacgg ctgtcgagaa
    69301 ctattatggc agaaacgatc atcggccagt tcagtaagtg agacaagtat ctttaataaa
    69361 taacacattt taataagtag ttgaacacat aactacctta cttgcaaagg cttaacaata
    69421 tctatacagg tacaacaaaa aactgtccac caattttctt tggcggacag ttttcactta
    69481 aaaataaaga agcaactatt cgtaaattat gctaaaatag cagtagaacg cgtcctgagg
    69541 gaatcgaacc cccgtctcaa gaaccggaat cttacgtgat atccactaca ctaaggacgc
    69601 aagcactggg ataattgtaa ctaaacagat aaaaaattac aaggggattg gacaaaaaaa
    69661 tgaaatttga gcaaaatttt tcacaaaaac agcagcagac acagaaacta gccatgacgc
    69721 aacagttgca acagtccatc caagttttac aatataacag tgaggagctg ttggcattcg
    69781 ttgagaatca agcaatggaa aatccattgg tggaagttgt tgagccggaa tggcagccag
    69841 attacataaa agcttcttca tcttcttatg aaggagaaga gacaaattac ctaaatcaga
    69901 ttcctgatac aaaagggtct ttatttgatt cattgatcga acaagtccat ttgaattacc
    69961 gagatacttt tttgaggaaa atcgttcttt atcttgtaga atacatcgat ttgaacggat
    70021 ttttgacgat tagtttagaa gaagccggaa aagagattgg agctacgcct atccaaatgc
    70081 tcgatgcgtt gactctgatt cagcaattag aaccagctgg agtaggtgca cgtagtttgc
    70141 aagaatgtct catgcttcaa acagaacgag atgattatgc gccggaatta gcttatatcg
    70201 tgttggaaga atgttttgag gaactggtgg agcgaaaatg gaaagaaatc gcacaacgtt
    70261 tttctgttga tctacatgct gttcagcaga tattcgacta tttacaaacg ttgagtccaa
    70321 gccctggccg gattttcgac cgtagcagcg aattgtacat tattcctgat gtccgggtat
    70381 tagtggacga cgataaaaat gtacaagtta tctcaaaccg aaaaaatcag ccaaatatac
    70441 gctttcaaga gagttacttc aaacaaatgt cacaacaagc agataaagaa actgaaaatt
    70501 atttaaaaga acggaaacag gaattcgaat ggttgaagaa aacgatccta caacgtgggg
    70561 atacaatttt gcgggttgct caagtcattg tttcccggca aaaagctttc tttatcgaca
    70621 aggaacgtcc aatcaagcct cttacgttaa aggaagttgc aactgagata gatgtgcatg
    70681 aatccacggt cagtcgtgca gtcaacggga aatatttgga aacagacttc ggtgtatttg
    70741 aattaaaaaa atttttcact acacgaattc ctacgaatag tacagaacag acagaagacc
    70801 tttcagcaga tacagcgaag aaaaagctgc aggaattagt cgatcaagaa gacaaaaaca
    70861 agccgttgtc tgaccagaaa ttggttgaat tgctgaaaaa agatgagata gcgatctcaa
    70921 gacgaacagt agcaaaatat cgtgatttat taggaatccc gagttcatca aaaagaaaac
    70981 gttatgataa ctaatcagtc aaaagactag aaaaagtatt tcactttttc tagtcttttt
    71041 agttgaaaaa cgagagcaaa actgttagac ttacagtcgt gaggttgaga tttcccttta
    71101 ttgatgtgaa attcgaatag ttaatttctt tttttatcgt atatgggtcg ttttaagtct
    71161 acattggacg taaagtgtcc aactaaggag atcgctatgc tggatgaaat aaaaatgatt
    71221 gaatctgttg caccagatat tatcgaagtg ttgcaagaac gctataaaat tttacgaaat
    71281 atctattgga tgcagccgat tggacgtcga agtttatcgg aaagtatggg gatcacagaa
    71341 cgagtattgc gaacagaaac agatcttttg aaaaatctga atttgatcga cacctcaaaa
    71401 agcggtatga cgctgacaac taagggcgaa gaagtttatc aaagccttga aaacttcatg
    71461 gatcagcttt taggtacgca tcaaaccgaa caacagttgg cagaatattt tggtaaccaa
    71521 cgctgtatcg ttgtttcagg aaacagcgaa gagcagacca aagtggctga tgcattcggc
    71581 gaagcgctga gcgaagccct agaccgacta cttccagaag gcgagaatat catcgctgtg
    71641 atgggaggaa ctactatggc tgtggtagcc gaacaattgt ctaatctgga aaataagaaa
    71701 cggcataacc tgtttgtccc tgcaagaggc gggattggtg aagcgataac tgtacaggca
    71761 aattctgtca gtgcaagaat ggctgctaaa gcaaacggta accaccgtgc actttatgtt
    71821 ccggaacaat taagtctggc aacttacaat tctttactca atgagccatc tatccaagaa
    71881 gtcttaaact tgattagtga agcgaattgt gttattcata gtattgggcg tgcattacac
    71941 atggcagcac gtcgtaagat gactgaaaaa gaattagtca tgctgaaaca agcaaatgct
    72001 gttgcggaat cttttggcta ctttttcaat gaaaaaggtg aagtcgttta taaggtccct
    72061 agaatcggca tagaattgca agatttggaa aaagtcccga tcatcatggc aatcgcgggc
    72121 ggaaaatcaa aagctaaagc aattcgagct tatatgaaaa atgcaccaaa acaaacgtgg
    72181 ctcatcactg atgaagctgc tgcaaatgag attttaaaag gggtaaccct ttaaaataaa
    72241 tatttttttg attttcataa ggaggaaatc ttaaatgaca gttaaagtag gtattaatgg
    72301 ttttggacgt attggacgtc tagcattccg tcgtatccaa gatgtagatg gaatcgaagt
    72361 agtagcaatc aacgacttga cagatgctaa aatgttggca cacttgttaa aatacgacac
    72421 aactcaagga cgtttcaacg gaacagttga agttcatgaa ggttcattca acgtaaacgg
    72481 aaaagaagtt aaagtattag ctaaccgcaa cccagaagaa ttaccatggg gcgaactagg
    72541 cgtagatatc gttctagaat gtactggttt cttcacttct aaatcagcag ctgaaaaaca
    72601 tttaacagct ggtgctaaac gcgttgttat ctctgctcct ggcggaaacg atgttccaac
    72661 aatcgtttat aacacaaacc acgaaacatt aactggtaaa gaaacagtta tctcaggtgc
    72721 ttcatgtact actaactgtt tagctcctat ggctaaaaca ttgaacgaca aatttggtgt
    72781 tgttgaagga ttaatgacaa ctatccacgc ttacacaggt gaccaaatga ctctagacgg
    72841 acctcatcct aaaggtgact tccgccgtgc acgcgctgct gctgcaaaca tcgttcctaa
    72901 ctcaacaggt gctgctaaag ctatcggttt agtaattcct gaattgaacg gtaaattaga
    72961 cggagctgct caacgtgttc ctgtaccaac aggttcatta acagaattag taacagttct
    73021 tgaaaaagaa gtaactgttg acgaaatcaa tgcagcaatg aaagaagctt caaacgaatc
    73081 ttacggatac aacacagacg aaatcgtttc ttcagatatc gttggtatga cttacggttc
    73141 attatttgat gctacacaaa ctaaagtaat gacagttggc gacaaacaat tagttaaaac
    73201 tgttgcttgg tacgacaacg aaatgtcata cactgcacaa ttagtacgta ctttagaata
    73261 cttcgctaac ttataagata gacaaacgta caagtgtaaa ttatagacat gtacatttaa
    73321 aagcggggaa gcaatcgcgc ttcttccgct ttttttgata ataagtttat tcaggaggaa
    73381 ataaaatggc taagaaaaca gtgaaagata tcgatttaaa agacaaaaaa gttcttgtcc
    73441 gtgttgactt caacgttcct ttgaaagacg gcgtgatcac tgacgacact cgtatcaaag
    73501 cagcattgcc aacaatcaac tacgttctag aacaaggcgg aaaagcaatc cttttctctc
    73561 accttggacg tgtaaaaaca gaagaagata aagaaggaaa atctttagct ccagttgcaa
    73621 aacgtctagg cgaattatta ggtaaagaag taacattcgt acctgaaact cgcggagaac
    73681 aattagaaga agcaatccgc aacatgaatg acggcgacgt tgtcgtattc gaaaatacac
    73741 gatttgaaga tattgacggt aaaaaagaaa gcaaaaatga tacagaacta ggtaaatact
    73801 gggcttcatt aggcgatgta tttgttaacg atgctttcgg tacagctcac cgtgcacatg
    73861 cttctaacgt aggtatcgct tcaactggta tcccaacagt tgctggattc ttgatggaaa
    73921 aagaaatcaa gttcatcggt gaagcagtag aagaaccaaa acgaccaatg attgcaatcc
    73981 ttggtggcgc aaaagtttct gataaaatcg gcgtaatcga aaacttgatt cctaaagcag
    74041 ataaaatctt gatcggtggc ggaatgactt acacattcta cgaagcaaaa ggaatcaaga
    74101 tcggtaactc tctagtagaa gctgataaag tggaactagc aaaagaatta atcgaaaaag
    74161 caggcgacaa acttgtcttg ccaatcgaca atgtctgtgc accagaattt tcaaatgatg
    74221 ttgaaacaca agtaatcgaa ggagacattc ctgatggttt gatggcttta gacatcggcc
    74281 cagcatcagt aaaattattt gctgatacat tacaaggtgc aaaaacagtt gtttggaacg
    74341 gacctatggg tgtatttgaa atgagtaact ttgctaaagg tacaatcggt gtttgtgaag
    74401 caatcgctaa cttagaaggc gcaacaacaa tcatcggtgg cggagactca gctgcagcag
    74461 cagaacaatt aggatttgct gacaaattca cacacatctc aacaggtggt ggcgcaagct
    74521 tggaattgct tgaaggtaaa gaattaccag gactagcagc aatcaacgat aaataaacga
    74581 ccaagtttgc tcaattctga cgagtaatcg atcaaaaggt gaagggagtt ttcatgactc
    74641 cctagccatt tttcaataaa ttaaggagtg tgttcctcaa tgcgtaaacc tattatcgca
    74701 ggtaactgga aaatgaacaa aactgcatca gaagcaaaag aatttgctga agcagtaaaa
    74761 aacaaaatcc cttcaaatga caaagttgat tcagtcatcg gatctcctgc attattctta
    74821 caagaattaa tgacatcagc tgaaggtaca gaactaaaaa tctcagcaca aaactgctac
    74881 tgggaaaact caggtgcgtt cactggtgaa acttcaccag cagctcttgc tgatctaggt
    74941 gttcattatg tgatcatcgg acattcagaa cgtcgtgaat atttccatga aacagacgaa
    75001 gacatcaata aaaaagcaaa agctatcttt gcaaatggta tgacaccaat cctatgctgc
    75061 ggcgaatctt tagaaaccta tgaagcaggc aaaactgctg aatggatcga aggacaaatt
    75121 acagctggtt tagctgatct ttcagctgaa caagtgtcaa acatggttat tgcttatgaa
    75181 ccaatctggg caatcggtac tggtaaatca gctgacgcac aaatcgctga cgaaatttgt
    75241 ggagttgtac gtcaaacagt tgaaaaactt tatggaaaag aagtatcaga agcagttcgt
    75301 atccaatacg gcggttctgt aaaaccagaa aatattgctg aatacatggc aaaagaaaat
    75361 gttgacggtg ctttagtcgg tggcgcaagc ttgcaagcag attcattttt gtcattgtta
    75421 gacgctgtaa aataattgtt gaaaatttgc gaaaaatgca agaaatgttt gcactaatga
    75481 gcaaaattaa ctaaaatcgt atatgaggga aatcccttag gaaaaaacaa actcaaagga
    75541 gagacaaaac atgtcaatta ttactgatgt ttacgctcgc gagatcttag actcacgtgg
    75601 taacccaaca atcgaagtag aagtatacac agaaagcgga gcttttggcc gcggaatggt
    75661 tccttcaggt gcttcaactg gtgaatacga agcagttgaa ttacgtgacg gagacaaagc
    75721 tcgttatggc ggtaaaggtg taactaaagc tgtcgacaac gtaaacaaca tcattgctga
    75781 agcaatcatt ggctacgatg ttcgtgacca aatggctatc gacaaagcta tgatcgcttt
    75841 agacggaact cctaacaaag gtaaattagg tgcgaacgct attttaggtg tttctatcgc
    75901 tgttgcacgt gcagctgctg actacctaga agtaccttta taccactacc taggcggatt
    75961 caacacaaaa gtattgccaa caccaatgat gaacatcatc aacggcggat cacatgctga
    76021 taactcaatc gacttccaag aattcatgat catgcctgtt ggcgctccaa cattcaaaga
    76081 agctctacgt atgggtgctg aagtattcca cgcattagct tcaatcttga aagctcgcgg
    76141 attagcaact tctgttggtg acgaaggtgg atttgctcca aaccttggtt caaacgaaga
    76201 aggttttgaa gtaatcatcg aagcaatcga aaaagctggc tatgtacctg gtaaagacgt
    76261 tgttcttgct atggatgctg cttcttcaga attctacgac aaagaaaaag gtgtttacgt
    76321 actagctgac tcaggcgaag gcgaaaaaac aactgacgaa atgatcaaat tctacgaaga
    76381 attagtttct aaatatccaa tcatctcaat cgaagatgga ttagacgaaa acgactggga
    76441 cggattcaag aaattaactg acgtattagg cgacaaagtt caattagttg gtgacgactt
    76501 gttcgtaaca aacactcaaa aattatctga aggtatcgaa aaaggaattg ctaactcaat
    76561 ccttatcaaa gtgaaccaaa tcggtacatt aacagaaact ttcgaagcta tcgaaatggc
    76621 taaagaagct ggctacacag cagttgtatc tcaccgttct ggtgaaacag aagattcaac
    76681 aatctctgat atcgctgttg caacaaatgc tggccaaatc aaaactggtt ctctatcacg
    76741 tactgaccgt attgctaaat acaaccaatt actacgtatc gaagaccaac ttggtgaagt
    76801 tgcagaatac aaaggtttga aatctttcta caacttaaaa aacaaataat tcttttctaa
    76861 tgaagataca tgatcgacta ttgaaagata gtcgagtatg atcacttagt taagtaatcg
    76921 aactctctac ttgctgaaat atcagcaggt agagagttt
//
LOCUS       DAYYBH010000006        69321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000006 DAYYBH010000000
VERSION     DAYYBH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.006"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..310
                     /locus_tag="ACHAUG_000447"
     CDS             161..310
                     /locus_tag="ACHAUG_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8479567.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            321..1835
                     /gene="dltA"
                     /locus_tag="ACHAUG_000448"
     CDS             321..1835
                     /gene="dltA"
                     /locus_tag="ACHAUG_000448"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8479568.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1832..3037
                     /gene="dltB"
                     /locus_tag="ACHAUG_000449"
     CDS             1832..3037
                     /gene="dltB"
                     /locus_tag="ACHAUG_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8479569.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3082..3315
                     /gene="dltC"
                     /locus_tag="ACHAUG_000450"
     CDS             3082..3315
                     /gene="dltC"
                     /locus_tag="ACHAUG_000450"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8479570.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3318..4586
                     /gene="dltD"
                     /locus_tag="ACHAUG_000451"
     CDS             3318..4586
                     /gene="dltD"
                     /locus_tag="ACHAUG_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8479571.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4727..5809
                     /locus_tag="ACHAUG_000452"
     CDS             4727..5809
                     /locus_tag="ACHAUG_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8479572.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5809..6612
                     /locus_tag="ACHAUG_000453"
     CDS             5809..6612
                     /locus_tag="ACHAUG_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8479573.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6682..7422
                     /locus_tag="ACHAUG_000454"
     CDS             6682..7422
                     /locus_tag="ACHAUG_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8479574.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7433..8437
                     /locus_tag="ACHAUG_000455"
     CDS             7433..8437
                     /locus_tag="ACHAUG_000455"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8479575.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8482..10326)
                     /locus_tag="ACHAUG_000456"
     CDS             complement(8482..10326)
                     /locus_tag="ACHAUG_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479576.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            10537..11055
                     /locus_tag="ACHAUG_000457"
     CDS             10537..11055
                     /locus_tag="ACHAUG_000457"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8479577.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            11183..11866
                     /locus_tag="ACHAUG_000458"
     CDS             11183..11866
                     /locus_tag="ACHAUG_000458"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8479578.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            12175..12248
                     /locus_tag="ACHAUG_000459"
     tRNA            12175..12248
                     /locus_tag="ACHAUG_000459"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12209..12211,aa:Arg,seq:tct)
     gene            12407..12766
                     /locus_tag="ACHAUG_000460"
     CDS             12407..12766
                     /locus_tag="ACHAUG_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8479579.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            12889..14520
                     /locus_tag="ACHAUG_000461"
     CDS             12889..14520
                     /locus_tag="ACHAUG_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8479580.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(14582..15223)
                     /locus_tag="ACHAUG_000462"
     CDS             complement(14582..15223)
                     /locus_tag="ACHAUG_000462"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8479581.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    15409..15485
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            15505..15813
                     /gene="rplU"
                     /locus_tag="ACHAUG_000463"
     CDS             15505..15813
                     /gene="rplU"
                     /locus_tag="ACHAUG_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8479582.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            15840..16184
                     /locus_tag="ACHAUG_000464"
     CDS             15840..16184
                     /locus_tag="ACHAUG_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8479583.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            16197..16490
                     /gene="rpmA"
                     /locus_tag="ACHAUG_000465"
     CDS             16197..16490
                     /gene="rpmA"
                     /locus_tag="ACHAUG_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8479584.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            16924..19731
                     /locus_tag="ACHAUG_000466"
     CDS             16924..19731
                     /locus_tag="ACHAUG_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8479585.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            19922..20413
                     /locus_tag="ACHAUG_000467"
     CDS             19922..20413
                     /locus_tag="ACHAUG_000467"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8479586.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            20516..21505
                     /locus_tag="ACHAUG_000468"
     CDS             20516..21505
                     /locus_tag="ACHAUG_000468"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8479587.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            21538..22341
                     /locus_tag="ACHAUG_000469"
     CDS             21538..22341
                     /locus_tag="ACHAUG_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8479588.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            22362..23276
                     /locus_tag="ACHAUG_000470"
     CDS             22362..23276
                     /locus_tag="ACHAUG_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8479589.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            23359..23733
                     /locus_tag="ACHAUG_000471"
     CDS             23359..23733
                     /locus_tag="ACHAUG_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8479590.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            23872..24117
                     /locus_tag="ACHAUG_000472"
     CDS             23872..24117
                     /locus_tag="ACHAUG_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8479591.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            24289..25353
                     /locus_tag="ACHAUG_000473"
     CDS             24289..25353
                     /locus_tag="ACHAUG_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8479592.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            25599..26024
                     /locus_tag="ACHAUG_000474"
     CDS             25599..26024
                     /locus_tag="ACHAUG_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8479593.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            26017..26466
                     /gene="nusB"
                     /locus_tag="ACHAUG_000475"
     CDS             26017..26466
                     /gene="nusB"
                     /locus_tag="ACHAUG_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8479594.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            26563..27417
                     /locus_tag="ACHAUG_000476"
     CDS             26563..27417
                     /locus_tag="ACHAUG_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8479595.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            27418..28755
                     /gene="xseA"
                     /locus_tag="ACHAUG_000477"
     CDS             27418..28755
                     /gene="xseA"
                     /locus_tag="ACHAUG_000477"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8479596.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            28760..28984
                     /locus_tag="ACHAUG_000478"
     CDS             28760..28984
                     /locus_tag="ACHAUG_000478"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8479597.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            28986..29876
                     /locus_tag="ACHAUG_000479"
     CDS             28986..29876
                     /locus_tag="ACHAUG_000479"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8479598.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            29885..30703
                     /locus_tag="ACHAUG_000480"
     CDS             29885..30703
                     /locus_tag="ACHAUG_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8479599.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            30810..31259
                     /locus_tag="ACHAUG_000481"
     CDS             30810..31259
                     /locus_tag="ACHAUG_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8479600.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            31277..32959
                     /gene="recN"
                     /locus_tag="ACHAUG_000482"
     CDS             31277..32959
                     /gene="recN"
                     /locus_tag="ACHAUG_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8479601.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(33014..33130)
                     /locus_tag="ACHAUG_000483"
     CDS             complement(33014..33130)
                     /locus_tag="ACHAUG_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8479602.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(33203..34159)
                     /locus_tag="ACHAUG_000484"
     CDS             complement(33203..34159)
                     /locus_tag="ACHAUG_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8479603.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            34330..34698
                     /locus_tag="ACHAUG_000485"
     CDS             34330..34698
                     /locus_tag="ACHAUG_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8479604.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            34868..35299
                     /gene="mraZ"
                     /locus_tag="ACHAUG_000486"
     CDS             34868..35299
                     /gene="mraZ"
                     /locus_tag="ACHAUG_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8479605.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            35320..36279
                     /gene="rsmH"
                     /locus_tag="ACHAUG_000487"
     CDS             35320..36279
                     /gene="rsmH"
                     /locus_tag="ACHAUG_000487"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8479606.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            36284..36685
                     /gene="ftsL"
                     /locus_tag="ACHAUG_000488"
     CDS             36284..36685
                     /gene="ftsL"
                     /locus_tag="ACHAUG_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8479607.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            36686..38878
                     /locus_tag="ACHAUG_000489"
     CDS             36686..38878
                     /locus_tag="ACHAUG_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8479608.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            38904..39866
                     /gene="mraY"
                     /locus_tag="ACHAUG_000490"
     CDS             38904..39866
                     /gene="mraY"
                     /locus_tag="ACHAUG_000490"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8479609.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            39880..41241
                     /gene="murD"
                     /locus_tag="ACHAUG_000491"
     CDS             39880..41241
                     /gene="murD"
                     /locus_tag="ACHAUG_000491"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8479610.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            41267..42355
                     /gene="murG"
                     /locus_tag="ACHAUG_000492"
     CDS             41267..42355
                     /gene="murG"
                     /locus_tag="ACHAUG_000492"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8479611.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            42370..43590
                     /locus_tag="ACHAUG_000493"
     CDS             42370..43590
                     /locus_tag="ACHAUG_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8479612.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            43746..45071
                     /gene="ftsA"
                     /locus_tag="ACHAUG_000494"
     CDS             43746..45071
                     /gene="ftsA"
                     /locus_tag="ACHAUG_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8479613.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            45094..46335
                     /gene="ftsZ"
                     /locus_tag="ACHAUG_000495"
     CDS             45094..46335
                     /gene="ftsZ"
                     /locus_tag="ACHAUG_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8479614.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            46352..47029
                     /locus_tag="ACHAUG_000496"
     CDS             46352..47029
                     /locus_tag="ACHAUG_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8479615.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            47043..47642
                     /locus_tag="ACHAUG_000497"
     CDS             47043..47642
                     /locus_tag="ACHAUG_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8479616.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            47723..47944
                     /locus_tag="ACHAUG_000498"
     CDS             47723..47944
                     /locus_tag="ACHAUG_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8479617.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            47993..48775
                     /locus_tag="ACHAUG_000499"
     CDS             47993..48775
                     /locus_tag="ACHAUG_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8479618.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            48796..49503
                     /locus_tag="ACHAUG_000500"
     CDS             48796..49503
                     /locus_tag="ACHAUG_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8479619.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            49765..52563
                     /gene="ileS"
                     /locus_tag="ACHAUG_000501"
     CDS             49765..52563
                     /gene="ileS"
                     /locus_tag="ACHAUG_000501"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8479620.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(52612..54138)
                     /gene="zwf"
                     /locus_tag="ACHAUG_000502"
     CDS             complement(52612..54138)
                     /gene="zwf"
                     /locus_tag="ACHAUG_000502"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8479621.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(54153..54800)
                     /locus_tag="ACHAUG_000503"
     CDS             complement(54153..54800)
                     /locus_tag="ACHAUG_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8479622.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(54984..55313)
                     /locus_tag="ACHAUG_000504"
     CDS             complement(54984..55313)
                     /locus_tag="ACHAUG_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8479623.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            55490..56218
                     /gene="sfsA"
                     /locus_tag="ACHAUG_000505"
     CDS             55490..56218
                     /gene="sfsA"
                     /locus_tag="ACHAUG_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8479624.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            56234..57247
                     /locus_tag="ACHAUG_000506"
     CDS             56234..57247
                     /locus_tag="ACHAUG_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8479625.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            57247..58524
                     /locus_tag="ACHAUG_000507"
     CDS             57247..58524
                     /locus_tag="ACHAUG_000507"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8479626.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            58587..61289
                     /locus_tag="ACHAUG_000508"
     CDS             58587..61289
                     /locus_tag="ACHAUG_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8479627.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            61441..61758
                     /locus_tag="ACHAUG_000509"
     CDS             61441..61758
                     /locus_tag="ACHAUG_000509"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8479628.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            61788..62108
                     /locus_tag="ACHAUG_000510"
     CDS             61788..62108
                     /locus_tag="ACHAUG_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8479629.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            62216..63676
                     /locus_tag="ACHAUG_000511"
     CDS             62216..63676
                     /locus_tag="ACHAUG_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8479630.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            63744..63965
                     /locus_tag="ACHAUG_000512"
     CDS             63744..63965
                     /locus_tag="ACHAUG_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479631.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            63996..64178
                     /locus_tag="ACHAUG_000513"
     CDS             63996..64178
                     /locus_tag="ACHAUG_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8479632.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            64178..64591
                     /locus_tag="ACHAUG_000514"
     CDS             64178..64591
                     /locus_tag="ACHAUG_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8479633.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            64714..65895
                     /locus_tag="ACHAUG_000515"
     CDS             64714..65895
                     /locus_tag="ACHAUG_000515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8479634.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(65966..66130)
                     /locus_tag="ACHAUG_000516"
     CDS             complement(65966..66130)
                     /locus_tag="ACHAUG_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479635.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            66284..66357
                     /locus_tag="ACHAUG_000517"
     tRNA            66284..66357
                     /locus_tag="ACHAUG_000517"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:66318..66320,aa:Arg,seq:cct)
     gene            complement(66426..67565)
                     /locus_tag="ACHAUG_000518"
     CDS             complement(66426..67565)
                     /locus_tag="ACHAUG_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8479636.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            67737..67871
                     /locus_tag="ACHAUG_000519"
     CDS             67737..67871
                     /locus_tag="ACHAUG_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479637.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(67864..68499)
                     /locus_tag="ACHAUG_000520"
     CDS             complement(67864..68499)
                     /locus_tag="ACHAUG_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479638.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(68612..69247)
                     /locus_tag="ACHAUG_000521"
     CDS             complement(68612..69247)
                     /locus_tag="ACHAUG_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8479639.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT        22722 a        11648 c        14605 g        20346 t
ORIGIN      
        1 gagaaattat aaagaaaaaa gcagtgattt gatgtgctat ttttataaaa gctatgtgac
       61 aaaactgtta gtaaaaaaac ggtttctgta agctagacac atgtttcttt ttgtcaaaat
      121 tagttacact ttgtgtgagg ttatatgagg agggaatcca atgaacaaat ggcgttctaa
      181 tccaaaattg aattattggg gaaaattcat cattcgaaca attttttata gtgcagtact
      241 gctgttcctg atttatctgt atcactataa aaatattcaa ggaggaactt ttatctataa
      301 cgaattttag gaggaagagt atggaaatca aaacgattat tgaagcaatc gatgattggg
      361 gaataaatga acctgatcga gttgcttatc aggcggatga gacgcatact tttggtgaat
      421 tgaaacaagc atcagacgca cttgcttatt atttagaaaa aaaggtagaa ggcgatggac
      481 caattgtcgt ttttggcaat ttggagtttg aaatgattgt ctcatttcta ggggttgtca
      541 aatctggcca tgcctatttg ccgattgaag agcatacacc caaggagcgt atcctatcga
      601 tctttcgggt ggctaaacca tctatggtca tcagtatcgg ggactggata gaagatattc
      661 cttcggttcc tgtcataaca aaaaaagaat ttcaagaaat caagaagata ccagtaggat
      721 tttatcccag aaatagcgta aaaggtacag aaaattttta tattattttt acttctggta
      781 ctactggaga accaaaaggc gtacaaatca gtcatgataa tctagtcagc tttgttcaat
      841 ggacgatgga agactttggg atcaagcctg gtatgcattt cttagcccaa gcgccatttt
      901 cttttgattt atcggtattt tctatttatc ctgcattagt ttctggcgga atgctgaagc
      961 cgttaaataa agcagttgtt caagactttc gtcaattatt cgcgacattg ccagccttga
     1021 agttgaatgt gtgggtttct actccatcat ttatggatat ttgcttgatg gaacctacat
     1081 tcaatggaga aaatgtgccg gaattggaca tgtttttatt ttgcggagaa gaattgacga
     1141 aaaaaactgc agaaagtcta cttgatcgat tcccaaatgc acggatatat aatacttacg
     1201 gtccaacaga agcaactgta gctatttcaa gtattcagat tgaccagcat gtactagata
     1261 gttatgaccg actaccgatt ggctacgtaa aaaaagacac gaaagtttct attgtccaag
     1321 aaggtaaacc tgtttcaaaa ggagaaacgg gtgaaatcat tattgcagga ccaagcgtat
     1381 caaaaggtta cctgaacaat cccgaaaaga cagctgctgc gttcttttgt caaaatgact
     1441 tagcaagtta ccatacaggg gatgcaggta gactagatga atatgggctt ttattttatg
     1501 aaggacgtat ggatcagcag gtcaaattac atggataccg gattgaactc ggggatatcg
     1561 aacattatct gctccaagac aaccgaatca aacaagctgt cgtcgtacca aaataccaag
     1621 gaacaaaagt acagcagctg gttgcttttg tcgtattaga aaacaaaacg aaacagccgg
     1681 attttcagtt gactaaatct atcaaagaac aattactaga agttgtaatg gattatatga
     1741 ttcctcagaa gttccattat gtcgatctgc tgcctcagac agttaatgga aaaatcgatc
     1801 gaaaaaaatt aacagctgag gtgaatcctg aatgatgtta cctcatatga tcccttatgc
     1861 agagccgttt tatttttttc ttttagccat tgcgcttgta ccgatcgttc tatctttatt
     1921 gatatgggaa aaaagattac cagtttatca atcagcagtg acacttttct ttttatttgt
     1981 cagctttgga ggtaattatt ggcaacaggg agcggcgctg attgggtatg ttttgtggca
     2041 gacgatactt gtatggggct attttgttta tcggcaaagg aaaaatcaga caggatggtt
     2101 ttttgccgcc gttattttag cgataattcc actgttttta acgaaagtga ctcctttttt
     2161 tgaacatgga aaagcatcgt tacttggttt tttaggaatc tcttatttaa cctttaaatc
     2221 tgtacaaatg atcatggaaa taagagatgg catgatcaaa acatataagc catttcgtta
     2281 tatccagttt ctgctgttct ttccaacaat ctcttctgga ccaatcgatc gctataggag
     2341 atttgaaaaa gatcagctcc atccgcctgt tcgtgataaa tatgttgatc ttttgggaaa
     2401 aggcgtccaa aatatttttg tcggtttttt atataaattc attatcggtt attattttgg
     2461 tcaagtactg cttcctgtag tgggacgcat tgccttacag catggtggta tttcatgggc
     2521 attagtaggt tatatgtacg tgtacagtat gtatttgttt tttgattttg ctggctatag
     2581 cttatttgca gtcggtacaa gctacatgat gggatatcag acaccaatca atttcaataa
     2641 acctttttta tcatggaata tcaaagaatt ctggaatcgt tggcatatga cgctgtcctt
     2701 ttggttccgt gattatgtat atatgcgatt gatgttcttc atgatgaaga aaaaattttt
     2761 taaaagcaag atcgtaatgt caaatatcgg ttattttgct ttgtttcttc taatgggtat
     2821 atggcatgga ttgacgtggt actacatcgt atatggactg tatcatgctt gtctgatctg
     2881 tctgacagat gcatggatcc gatttaagaa gaagcataaa gagcagttgc cttctaacaa
     2941 atttacgcat ggcttagccg tatttttgac atttcaagca gtatgtttta gcttcctgat
     3001 tttttcagga tttttagata aacttttttt tagctaacga atagtgacat attaatttat
     3061 ataacaaagg agaaaaggaa aatggaagaa caaattttat caatcttaga agaaataaca
     3121 ggaacagatg aagtaagaaa aaatcgtgat attgatctat ttgatgaagg gttgcttgat
     3181 tctttagcat ctgttcaatt attagtcgag ctagacggag agctggatat ccaagtccct
     3241 gtatctgaat tcgagcgaga ggattggtgc acaccgaata aaatcattca acaagcaact
     3301 gcattgaagg gttgagtatg aaaaagaaac tttttgctat tttcggcccg gttgttttag
     3361 ctgttggctt actgtttgtg ttttttacct cctcctatca agtagatatc tctaatccag
     3421 aaacgattag acgtgcctct tcttccatgt cagtgaacgt gttgaaagga gatgcgataa
     3481 aaaattatgc attatctgaa aaacaatata ttcctttttt tggctcaagt gaattaagca
     3541 gaatcagtcc atttcatccg tctgttctgg cagaaaaata ccagcgaaac tatcgtccat
     3601 ttttgctggg agcacccggg acacaatcgt taagccaata tatgatgatg cgttctgcag
     3661 gagatgctat gaaaaacaag aaagtcgtct ttattatttc tcctcagtgg ttcgttaaaa
     3721 atggtgtaaa aactgattac tttaatacgt attattctga acttcaaaca tacgactggt
     3781 tgttttcaat gaagaaagta acgcctgctg atcgctattt agctagaaga ttattaactt
     3841 tctcaaaagt aaaagagaat gatacgttga cggcgattct gcaaacgatc aaaaaaggga
     3901 agctaccttt acctgaatct ttgaatcagc tgcgcagcca atggaacatg ctgaagagag
     3961 aggatgaagt attcagcaat attgggttga aagatcgaca gcaaaaaatc gatcatgagt
     4021 caaaacgatt accaaaacaa taccaagaaa cagaattatc cattttagca aatcaaattg
     4081 gagaacgaga aacgacgaat aatccattcg ggttgaaaaa cgatttttat acgcatcgta
     4141 tccgagccca cgagccagag ctcaaacagt cacaaaaaaa ttgggattat cgtttttcgc
     4201 cagaattttc tgattttcag ctggtcttgg atcaattagc aaaaaaccat aacgaagtat
     4261 tgttcatcat cccaccggtt aacgagaaat ggagcgatta taccggacta tctcaagaaa
     4321 tgttacaagg atttgccaaa aagatcaaat ttcaattaaa tagccaagga ttcaatcgaa
     4381 tagctgattt tgtcaatcaa gcgggtacca actattttat ggaagacacg atccatctag
     4441 gctggaaagg ctggttggct gcagatcaac agatcagacc gtttttagaa gaaaatcata
     4501 ttactgctag taagtatcat cttgacgatg ctttttttag caaatcttgg caacatcaaa
     4561 taccagataa actacaatta aaataaagaa aacaggcgag tgttgctcgc ttgttttctt
     4621 tatttttgaa tattctgaat aaaagataaa aataccaagt ttgcctgaac aaaaatcata
     4681 agcaatggta taatcacaat atgaatatag aaatggggga aaacatttgg atacaaaaga
     4741 agaacagcta cttattgatt taacttctgc aaaaggagta cctggaaatg aagaagaggt
     4801 aagagaagta ttcagagaat atgccaaacc attcgctgat gatatttttt atgacggttt
     4861 aggcagtgtg attgccaaac atggtgctgg cggaggcccc aaggtcttta tttctggaca
     4921 tatggacgaa gttggtttta tggtaacaaa aataaccgaa aaaggattct tggaattcca
     4981 aactcttgga ggttggtggg ggcaagtcat gcttgctcag caagttgaaa tcaaaacacg
     5041 tgaagggaaa atcgtccacg gtgtgattgg ttccaaacca ccacatgttt taacacctca
     5101 agtaagaaat aaaccttatg aaataaagga tatgttcatt gatattggcg catcaagcca
     5161 agaagaagcc aaagagtggg gcatacgtcc tggagatatg gtcactccat atattgaata
     5221 caaacgaatg aatggcagta aatatttact ggcaaaagca tgggataatc gtatcgggac
     5281 agctgtttct ctgcgagtat tagagaatct atctaaagaa gcacacccaa atgttttatt
     5341 cgcaggaagc gatgtgcaag aagaagtagg cttgcgaggt gctcgaacaa gtacacacct
     5401 tgtaaatccc gatattgctt ttgctttaga tactggtaca gcaggagaca ctccaggaat
     5461 gacaccaaaa gaagcagatt cgattttagg aaaaggccca cagattttga tttttgatgc
     5521 atctatgatc ccgcataaaa aattattgaa ttttgttatt gatgtagcag aggaaatgga
     5581 tattccattc caatatacag tgatttctgg cggaggaact gatgcaggac aaatgcattt
     5641 gacaagagat ggtgttccgt ctgtagccat taccgttcct gttcgttatc tgcattccca
     5701 tacatcagtg attcatgaag atgattattt gaatacagtg aaactagtca cagaattagt
     5761 gaaacggctt gatagtgaaa cagttgaaaa aattcggagt tatgaataat gaataataga
     5821 atacctattt ctgaatatcg gaaaaaagca cgagtttctt tggaaaatca atggggaatc
     5881 aatacttggc ttgttttctt atctataata tttacgagcg tcatccagaa cttttttgac
     5941 ggtttcagtc cttttccgga agaaagcatg cagcagcact tattgagttt tctagtcgat
     6001 aatttacttt tattcgcatt caactatgca ttgttttaca ttgcgctagt agttgtacgg
     6061 ggtggcagac caaaaagtaa ccttttattt tcagttttcc aaagaaagta ttttggttcg
     6121 atacttggca tcaatttgat caattcagtc atcaactggc tactcaacag tttaatcttt
     6181 ctgcccatgt ttttaatcag cggagtgagt acttatatgc agctgctttt cagttcaaat
     6241 gcggtaccta cgggtgtcat gccagaagat tttcttgata tcagctttgt tttatcgatg
     6301 atagggatga tttttgtttc agcactcatc atgagtattg ttggcggttt attccaattt
     6361 gctgcatgga caaagttcga ctatcctgat ttgtcggtta ttcaatgttt gaaatatggc
     6421 tggtttttat tgaaagatag aattggtacg tatattttgt tgcagctgtc ttttatcggc
     6481 tggtatttgc taggagcaat cgctttgttc atcggtcttt tgtgggtcat tgtttatgtg
     6541 aatgtgtcag ttgcagaatt ttatgaacaa gcacggattg aaaaaggagg tccagacgcc
     6601 tattttactt agcatgtaag cagtttattc atcaaaaaaa taacttttgt ggtaggataa
     6661 atctttgagg gggaatgtac gatgaaatta actgttttag gatgcttagg agcgtatcct
     6721 tataaaaatc aagggacaac aggttatttg cttcaatctg acgggttcaa tttgttgatc
     6781 gatgcaggta gtgctacgct gatcaaatta caagaacacc ttgatccact tgatctagat
     6841 gctgtgatcc ttagccatta tcatcatgat catattgctg accttggtgt attacagtat
     6901 tactggcaac ttcatcctaa gagaaatccg gagacattgc tttcaattta tggacacaca
     6961 aaagattcca ttcactttga agaattgacg ttggaaaatg tctcaaaagg acatccttat
     7021 tttgaagctg aagaattaaa aattggtcct tttctgatta cttttatgga aacaatccat
     7081 ccagtagtat gttacgccat gcgtatcgta gaagaaacaa cggggaaagt attcgtattt
     7141 acaggtgatt caggttattt ggaaagtttc gctgattttg cgaaagatgc tgatttgttt
     7201 ctagcagata cctatctttt tgaaggaaat gaacatcatc atgcccattt cacttcaaaa
     7261 gaagctggag aacttgcgaa atcagctaac gtaaagaaac ttgtacttac acacctacct
     7321 cagtttggtg atttagaaca attgaaaaat gaagcgcaaa aggcagcagg caaagggatt
     7381 gatatagaac ttgctgcagt tgacaaagtg tttaacattt aggaggaaga acatgatttt
     7441 tgtaccaaat gaaaaccacg acccaagaat caatttagca attgaacaat atttgctgca
     7501 agaaatgcct ttagatgaac ctattttgct cttttatatc aatgaacctt caatcatcat
     7561 cggcagaaac caaaatacgc ttgaagaaat caatcgagat tatgtggaag aaaatggcat
     7621 tcatgttgtc cgtcgcttaa gtggaggcgg agctgtctac catgattttg gaaacctgaa
     7681 ctttagtttt atcatgcctg atgatgggga ttcattccgt gactttgcga aagtaacaca
     7741 gccaatcatc caagcacttc atgaattggg tgttgctggg gcggaattga aaggccgaaa
     7801 tgatctagtc attgatggca tgaaattttc tggcaacgcg atgtatgcta ccaacggtcg
     7861 gatgtttgct catggaacaa ttatgttcga ctctgatatc aatgaagtag tcaatgcatt
     7921 aaaagtaaaa aaagacaaaa tcgaatcaaa aggaatcaaa tcgatccgtt ctcgtgtaac
     7981 gaatatcaaa ccatttcttc cagaagaaaa gcaaaaaatg acaacagaag aatttcgtca
     8041 agaaatcttg ttgaaaattt tcggcgtaga gtcagtagaa gaagtaaaaa cgtacgaatt
     8101 gactgatgaa gattggaaaa aaatcaatcg aatctctgag gaatattatc gaaattggga
     8161 ttggaattac gggaagtctc cagatttcaa tttctctcgc caaaaacgtt tttctatcgg
     8221 ttcgattgaa gtccatttga atgtctcaga aggaatgatc aaagaagcaa aaatctttgg
     8281 cgatttcttc ggactaggag atatctctga cgtggaaaaa aaattggttg gacagaaata
     8341 tgataaagca tctctatcaa aaatagtcag ccaaattgat gtgaagaaat attttggtgc
     8401 gatagaacca gaagaattgc ttaccttact ttattgaaaa taaaaaatag cggaatccac
     8461 atatatggat tccgctattt tttatacagg attcgggtaa tttcgagaga gttgaacagt
     8521 gatccgatca atgaaagtat ctttttcctg cgttagtgtt cctgtgtaac gatccgctac
     8581 gtctttcatc atcattaagt caaaacgttc ctgatcatct aattcttctg tgatgcctcg
     8641 actaaatagg tttgtatcgt aatgtgcact atgttcgcaa caaatgacga aatgttcctc
     8701 ttgttcgtgt atcgacaaat aaatttttcg atacagtgga ttttcgatgg catgagtttc
     8761 tctaaaggaa tgctctaaag cgtggatcaa aagttgagcc agatcgtcat cgttgatagg
     8821 aagttcttcg ggaagatctg cttggaaggt tatctgaata ttccgtttgg cagctaactt
     8881 ctggtaacgc gctaagataa gatttgtcac ctggtggttg caaaaagaac acagcatttc
     8941 gaaatttttt gcttcttcta atatcttttc cacatagttt ttagcttttg tttggtccgt
     9001 atgcattaaa tcttgcagat tctccatctc ttggacaaat tccaggcgaa gtgtatttac
     9061 ttcttggata tgctgtcgaa gctgctcata atgttgctcc aacaaatcat ttttgatttt
     9121 taagaaatct acttgtctcc gatctttgtc ggtatttaag atgtgatcga ttagattgta
     9181 gctgataata acgattagaa tgataatata taaaatcgta gaccaattga tcgttgtttg
     9241 atagatacct aatgccgttt ggatgtaaat caagcaatgg ataatggcta ccaagacgat
     9301 ccaaggtgta caagctacat aaaaacggtt attcttatat gcttcgccaa tgcatgcaag
     9361 tgaagtgact aaagtgctta taacactagc aatattagca atcggcatta attcaggaag
     9421 ttctgcagga gtaaaagttg cataaagcag aacgccgata aaaatcaaaa gttggaacgt
     9481 acaaaaaatt ccgtaccatt ttttatagta aaacattcgg ctccaatagt agcagatgat
     9541 gaaagctgac gttaagataa taaataaatg agataaaaaa gaatgaacga atggttcaaa
     9601 aagaatacca cttaaaatat cttctgatac agcttcaaat cctaatgcca aagcaaagca
     9661 acttaagaga ataagtgagt attcaagacg tttttgttta tacatcaagt agatcgtata
     9721 gaaaaaggtc cctatagcga taaaaacagc gattatcatc gtcaatactt gcggcactga
     9781 ttgtgaataa ataaaactaa tcaatggtcc agttgttcct agataaactt tcggtggcag
     9841 tccagcatag ttctgataag gagatgagac tgtaagggtc aatttttttc cgatataatt
     9901 tgctggtaaa tctatcacag ccaatgagag tccgggattg ctttcttcct gataatcttt
     9961 ccgttggtat aaaatctttt cgcctagata tacagtaatc cattgatggt ttcctttgat
    10021 tagtaaattt gcttcgtcca acggtttttc aagtgtcgtt tccataatga atgtttcgtc
    10081 tttatccacg gtaggaatcg tacgagtgta cttgctgtca aacttaaaat caggatcatc
    10141 ttttgtatag aaaacccacg aatcttttat ttctttcatt tgattgctgt ccgcaggaga
    10201 aaaaataaaa taaaagaaat aagcaattcc tgttaaaaat aacaatgata atataatgat
    10261 tatgctccac tttatacgta aataaaaaga ttctttttta ttatatatgt tattaaaaaa
    10321 agacataaag gtattccttt ctaaaacttg taatgttagg gtaaacgttt tttaaactaa
    10381 cgtcaattat ttataataaa aaatgagata aagatgagga aatagaatta gaaacatata
    10441 attattaaag ggaaacattt ttacagctat aatttgaaaa aatttatcgt tattatatga
    10501 ttaagaagga ttgaataaag aaagaagttg aaaacgatac ctgattacaa aaaaataact
    10561 tttataagtt ctataacgtt gattatattt ttaattctta ttcttctcgt cgtcttggtt
    10621 ccccgaattt ttggtttgac tccttatatt attgaggaca aagaaatgag tccatattat
    10681 gcacaaggaa atctgatata tgtacgaccg aaaaatacaa atgagatttt agtcggagat
    10741 attattacat attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt
    10801 gaaaacaacc acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga
    10861 gtagtaaaaa agagaaatat tatcggtagt cccatctttc agattccata tattggacta
    10921 gtcctttcaa aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc
    10981 ctaacaggaa tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca
    11041 cagaaatttt tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc
    11101 tgaacatgaa acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata
    11161 atgtgtgaag aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga
    11221 agaactattg gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact
    11281 ggaagaagaa gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt
    11341 gaaattttta cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg
    11401 tttttcatca agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga
    11461 tcgtttcgat gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga
    11521 taaagtgact ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta
    11581 tgactttatt tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt
    11641 acgtttgtta agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat
    11701 cttgctccca gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga
    11761 atttcttcgt gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga
    11821 tggtgtgatc ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc
    11881 agcctgtaga taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat
    11941 atttgtaagt caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa
    12001 tgtagctcct gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa
    12061 aagattgatt tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc
    12121 gtattgacag aagcaattta attatctata attattgatg ttccgctgat tcttgtcata
    12181 gtagctcagc tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc
    12241 ctgtgacgct aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt
    12301 gccaatattg tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat
    12361 tataaggagc aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc
    12421 tagaatttta gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt
    12481 gagaactgat tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag
    12541 aaaatctgtt caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg
    12601 actactttat ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat
    12661 atctattttt tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa
    12721 acaaggtggg ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata
    12781 agaatttatc taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat
    12841 tctaatgaat ggtattttag aaaatttcat gaaaaaggag ttagataggt gttcaaaaat
    12901 tatgtttcct cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat
    12961 cttttggctg ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta
    13021 tcaagcggag ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt
    13081 ggcggtttat caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc
    13141 ttgatgtcta ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg
    13201 ggtctatttt tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca
    13261 gcacccgtaa ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc
    13321 gataatttat ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat
    13381 caaacgatag gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta
    13441 gggatgttta tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc
    13501 attttggcaa cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt
    13561 ccacaaacat tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg
    13621 caacaagtgg cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta
    13681 tgtggtgctt cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg
    13741 gcgcaaggaa ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc
    13801 atcgcccgta caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata
    13861 catgcagttg tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca
    13921 atgccagcac ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg
    13981 atcaaggagc tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg
    14041 atttgtacag ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt
    14101 gtattttttg ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa
    14161 agaatggggc tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt
    14221 ccactgtttt ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac
    14281 gatggtgtca ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt
    14341 tttgatgaat ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg
    14401 cagccagaag tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat
    14461 caaacagttg ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa
    14521 aacaatgaaa aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt
    14581 tttaatattg ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa
    14641 gggagcgatt gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt
    14701 ctgaccattc gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt
    14761 gaccatgatt tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc
    14821 caagtgtcgc aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat
    14881 gcgcaaatac ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg
    14941 tccgtttatt gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat
    15001 acacctcttc tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt
    15061 gaccgctaaa aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca
    15121 tcagtttagg atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca
    15181 ttcttaaaga agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt
    15241 cttttattat aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg
    15301 atagaaaagc agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg
    15361 actaacggtt gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa
    15421 ccgctcagaa caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta
    15481 tatcagaagg aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta
    15541 aaagttgaag tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa
    15601 gttgtttttg acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact
    15661 gttgcaggtg caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt
    15721 actttcaaat acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat
    15781 acaaaagtgg ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata
    15841 tgatcaaagg aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg
    15901 ggcatgctga tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag
    15961 cgatcagcac tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga
    16021 atgaagaaga aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa
    16081 acaatattgc tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg
    16141 aaaactcaga atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt
    16201 tgttatctat gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg
    16261 gccgtgactc agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag
    16321 gtggatcaat tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta
    16381 tcggtggaga cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag
    16441 gccgtaacaa aaaacaagtg tctgtttatc cagtagctca agaagcttaa ttgaatctta
    16501 tgattaaaga ggttgtgaca agacttttgt cacagccttt tttgtttata aaaataaaag
    16561 ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt tttgccaatt
    16621 tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata ttgtttgcta
    16681 tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta taaaaatcgt
    16741 atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt tatcaacaaa
    16801 acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat gttttgaagc
    16861 ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat tgaccgaatt
    16921 tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga tcaaggagta
    16981 acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag taaagatctg
    17041 aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt acgttacata
    17101 tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa
    17161 acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat ctttgcaaaa
    17221 atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc tgcgattttg
    17281 tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg aaagacttat
    17341 tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa
    17401 ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat gagtcattta
    17461 tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg gattatcgat
    17521 caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc tgaaggtcaa
    17581 gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga aacaacgaaa
    17641 tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg ttcttctcta
    17701 ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt
    17761 cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc aaaccgcacg
    17821 cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag tgtgacctat
    17881 ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg atttctaaat
    17941 catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg gattcgtagc
    18001 ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa aatattggag
    18061 ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga ctcctatgag
    18121 ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa gtcggatggt
    18181 ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca tctgaagtct
    18241 ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga atttgtagat
    18301 ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc tttaccatat
    18361 gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt tttgaaaaaa
    18421 atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc ttttgatttt
    18481 ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg
    18541 gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt acgggcaaat
    18601 caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt
    18661 atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt agacatgcct
    18721 ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca agaagtagat
    18781 gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt taattcgcaa
    18841 attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac ttctctagtt
    18901 ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt gcatgaatca
    18961 ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca ggaagcaaaa
    19021 cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg tacagcacaa
    19081 agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga attagaagtt
    19141 ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa agaatatcag
    19201 atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat tcctttagaa
    19261 cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga atcagaattg
    19321 gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg tgttgagttt
    19381 atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat gtggcaattt
    19441 tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt caaaatcaac
    19501 ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc attgactgtg
    19561 gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt ggcagcctca
    19621 gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat gtattatctg
    19681 ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg aatacacaaa
    19741 aacgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct ttaaattcaa
    19801 ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg
    19861 gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag gagttttgca
    19921 gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag tcgcaacagt
    19981 ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga
    20041 taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag caaatgtaat
    20101 aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag caaaagtgat
    20161 gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg tacagttgaa
    20221 gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta ccaattttgt
    20281 ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag aagtggagtt
    20341 agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac tagcgaaaat
    20401 caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt gtaatggtta
    20461 gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta taaatatggt
    20521 agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat ccggttcaat
    20581 gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga
    20641 tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag acgaagtgtt
    20701 attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat tattcgaaga
    20761 gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga ttgaagccta
    20821 cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca ttgaaacagc
    20881 aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac cgaaagcagc
    20941 agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg gagacggaaa
    21001 aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag ttgcgacagc
    21061 ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag tatcaaagga
    21121 tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat
    21181 tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata ccaaagcatt
    21241 gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg tagacatcaa
    21301 agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt
    21361 gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt
    21421 cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt tgaaaaaagc
    21481 aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag gcttatcatg
    21541 tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat ggaaggtatt
    21601 cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg tttagttacc
    21661 ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc tttaggttgg
    21721 gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc atcagcaatt
    21781 attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt
    21841 cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc tgttccaatc
    21901 gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac
    21961 atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc gttgctattt
    22021 atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt aacagatggt
    22081 atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat caacatgatg
    22141 gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc aatttctcaa
    22201 ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta cttgaacctt
    22261 tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga ccctttaggc
    22321 gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa gaaaaaatca
    22381 aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt caaggttcat
    22441 ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca gcaatcaaaa
    22501 aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg gaattcttta
    22561 atacacaccc atacattgct tctccaattt taggggttac attggctctg gaagaagaac
    22621 gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta ggtatgatgg
    22681 gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca atgctaggtg
    22741 cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc ttcttcttag
    22801 gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc tacaaagctg
    22861 gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca aaaggtgctt
    22921 caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca atcaaattct
    22981 taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg gataaattgc
    23041 cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc ttagcccttt
    23101 ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct ggtttagctg
    23161 cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta
    23221 tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt ttataatcca
    23281 taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg ttcataaaca
    23341 aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt ggtaattgat
    23401 gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtaa taatggtttt
    23461 gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga agaagtggat
    23521 tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc cattcaaacg
    23581 aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc
    23641 agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga tgtagtgaaa
    23701 cgtggtattc gtagtatgtt taaacgcaag taaaaaataa agaaaacttc taagaggagg
    23761 ttttctttat ttttttactt tgcagaagaa aatatgaaat agcaaaacca attatttgtc
    23821 ccctttaaat cccatatgct aaaattgatt agcaaagaag gaggttatat tatgagttgg
    23881 ttatggacat tgattgttgg agccgtgatc ggagcaattg caggtgcgat caccagtaaa
    23941 ggaaattcga tgggctgtgt aacgaatatc attgctggac tagtcggttc aagtattgga
    24001 caagcgattt tcggcgaatg gggaccaaaa gctgcaggta tggcacttat tccatctatt
    24061 ttaggcgcag tgattttggt agcagttgtc tcatttttct ttggaaaacg atcataagtt
    24121 tttacaaaat agtaaactaa aaggagttgc agcgagactg tgactccttt ttggctttat
    24181 ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg tacgaagaaa
    24241 agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat gatgctacgt
    24301 gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt ggttaccagt
    24361 ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc tgttattact
    24421 ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc agcgcaggct
    24481 caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc aaacttagta
    24541 cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt tttagaatat
    24601 tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat gatcgaagaa
    24661 ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg tcatattgcg
    24721 gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga gattgaagtc
    24781 gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc ttttgatacg
    24841 atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc
    24901 gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta cgtttccgat
    24961 atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat ttatcaaata
    25021 gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac tggtgtccaa
    25081 ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc atttggacat
    25141 tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc ttctcgtgca
    25201 gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta cttaccagga
    25261 atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg
    25321 actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa gtaagcgtaa
    25381 aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata cactatagaa
    25441 gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca tagcagtttg
    25501 cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat agagaattcg
    25561 gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa aaaaatttga
    25621 ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa
    25681 ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga acttttgcca
    25741 gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg
    25801 aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt tcagttccta
    25861 aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg acggatatcg
    25921 atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa
    25981 caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt atcaagacac
    26041 gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc agacctaaca
    26101 aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat caacgaagat
    26161 gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa
    26221 gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat caatcggatc
    26281 tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta tgtgacagat
    26341 gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt tagtgatgac
    26401 cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt agaagctggg
    26461 gcgtaagtcc tagctttttt ctatgaatca tcataaacct gttataaatc gggaaaattc
    26521 tatggtagaa tgaaaacaca aaaaatatct gggagtggtt agatggcaga attgattaac
    26581 ggaaaagaat tagctgaaaa aatgcaggca gagattgctg aaaaaatcca aaaattgaaa
    26641 gataatggta tccatcccgg attagtcgtt ttattggtag gggaaaatcc agccagccaa
    26701 atctatgtaa gaaataaaga acgtgtagca aaagaaatcg gaatccattc attggtagag
    26761 cgttatcctg agacgatttc tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa
    26821 gatcctagtt ttcatggtat attggtccaa ttaccattgc caaagcatat agatgaagaa
    26881 aagatcctgt tagctatccg ccctgaaaaa gacgtagacg gttttcatcc gatgaattta
    26941 ggcagactcc ttgcgggtaa accggacaag attccttgta ctccatacgg catcatgaaa
    27001 atgtttgaag cttattctat cgatccggcg ggtaaacgag cagtagttat cggacgaagc
    27061 aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac tgttactgtt
    27121 gctcattcta aaaccgaaaa tctggctgaa ctggcacaag aagcagatat tttagtcgta
    27181 gcaataggac gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata
    27241 gatgtaggga tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa
    27301 gtggagccta ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc aatgactatc
    27361 acgatgctga tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg tgaatagatg
    27421 gcacaagaat atttaactgt cactacatta acgaagtatt tgaaaagaaa atttgatgcc
    27481 gatccttatt tagaacgcgt atatttaaca ggcgaaatat cgaatttccg tttacgtgcc
    27541 aatgcccatc aatatttcag cttaaaagat gaccatgcaa aaatatctgc tattatgttc
    27601 aaaggagcat tccaaaaatt acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt
    27661 gggcgtattt cattatatga agcgggaggt tcttatcaaa tctatgtaga gcacatggag
    27721 cctgatggaa taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag
    27781 gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc
    27841 cttaccagtc caagcggagc ggttatccgc gatatcatca cgacggtgaa aagaagatat
    27901 ccaattgtcc aattggtttt gttcccaaca gttgtacaag gaaaccaagc agcggacaat
    27961 gttgtcagga atatccagag agtagaagag atgggcaatt tcgatacaat gatcatcggt
    28021 cgtgggggtg gttcgattga ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg
    28081 atttatcaag ctaatacgcc ggtgatttca tctgtaggac atgaaacaga taccacgatc
    28141 gctgatttag tagcagatgt tcgggctgcc actccaacag cagctgcaga attagctgtt
    28201 cctgttttgt cagaagagtt gatgcgcatt caagaacggc aagctagatt gcagcaggcg
    28261 tttgtacgcc agatccagcg caaacaagaa cggttcgagc gtgctcgacg ttcctatgta
    28321 tttcggcagc ctgaaagatt gtatgaaggt cagaccatca aattagacca gttgaaacag
    28381 cgattatatc aagctacaca gcaaatttat catgcgaaag aaaaacaaac gatcgcatta
    28441 agccaccaat tggaacaagt ggctccgctt tatcgagtta aagcagctcg ccaagaaact
    28501 gcttatctag aaaaacgctt agaagagaaa atccaacaat atatgaaaca acagcagcag
    28561 cgttttgctc aagctgttca atctcttgat ttgttgagtc cattgaaaat catgggaaga
    28621 ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa
    28681 ggggatttgc tgacgatcca ttatcaagat ggaatcgtta aaggacaagt acaagagata
    28741 gacaaaggag aataaaaata tggaaaatcc aacttttgaa gagtcactgc aagaattaga
    28801 aaaaattgtc atgcagttag aacaaggaga cgttccatta gaatcagctc tagattcgtt
    28861 taaacgagga atggaattaa gcaaacattg ccaagataca ttagctaaag cagaagagac
    28921 cttgacgaaa atgatgacag aatcaaatga agaagttgat tttgacggaa acgaggaaag
    28981 ctgagatgag attaactgac ttttcagcta ttcatctacc tgctgtagaa aaagaaatct
    29041 tgtctttttt agatgagcat acaacggata gaggactcta tgatgcgatg agttattcag
    29101 tgaaagcagg cgggaaaaga atacgtccct tattactttt aacggccgtt gcttcctttg
    29161 atgagcctat tgatgttccc gtctatcaag tagcagctgc tttagaaatg gtgcatacgt
    29221 attccttgat ccacgatgat ttgcctgcga tggacaatga tgatcttcgt agaggaaaac
    29281 ctaccaatca taaagtattc ggcgaagcat tagctatatt ggccggagat ggtttgctta
    29341 caggagcatt ccagctaatc agtatggcac acttaggtaa ttctcctaag cttctgctgc
    29401 ttcagcaatt agctgtttgt gcgggatcac aaggtatggt tgctgggcaa gctgcagata
    29461 tcgagggcga atcgaaaaag ctttctttag aagaattagc atttattcat gaaagaaaga
    29521 cagggcattt gattcgttat gccttgctgg ctggtggcat attagcgaag caacctgaag
    29581 aaatcttatt gctacttcaa cgattagccg aacatttagg tttagctttt cagataagag
    29641 atgatctact cgatgtgatt ggtacaacga aaacattagg gaaaacagct ggtaaagatg
    29701 aacgtatgga aaaaaatact tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt
    29761 tggagattga gctgttatcc gccaacaaga tcatagacaa attagaagag gacgtttttt
    29821 cttttgatgg cgggctgatg cgccagatga tcaagcaatt tgatgtggag aaatgaggga
    29881 aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa caaggattat ttgaaacaag
    29941 agaacaagcc aaacgttcag tgatggctgg tttgatttac aacgaaaaaa acgagcggtt
    30001 tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag gtaaaaaact
    30061 tccttatgtt tctcgagggg gattaaaatt agaaaaagca ctcaaagcat ttgatctatc
    30121 tgttgaagga aaaacattgt tagatattgg tgcatccact ggcggattta ctgatgcagc
    30181 tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc agctagcttg
    30241 gaaaataaga caagatccta gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa
    30301 acccgaagac tttaccttag gaattccaga agttgctgtt atcgatgttt cttttatttc
    30361 actaaaactg atgctgcctc cattacatgc tattttaaaa gaagagggag aagtgattgc
    30421 tttaatcaaa ccccaattcg aagcaggcag agaagctgta gggaaaaacg gaattgttcg
    30481 tgaccctcag acccatcaaa aagttttaga agacattttg tcatttgcgg cacaaggtgg
    30541 atatgatgtg ctggaattaa gttattctcc gatcacgggg ggagaaggaa atatcgagtt
    30601 cttggctcac ttgaggaaag tacccgaaag cggaacaatc aattcagcta ttaatatggc
    30661 agaagtggtt tcaaatgcac acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat
    30721 caagtgagaa gtatgcggat tcttctttat tataccaagg aaataagcta tcattgatta
    30781 agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta
    30841 ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga atttgttgaa atcctgaaaa
    30901 gcaaaggaat atctgttact caagctacga tttccagaga tataaaggaa ctgaaactga
    30961 tcaaggtacc ggcttcagat ggcggctacc gttatagcat accagctgaa acaaacgaag
    31021 atgtaaatac taaattggaa aaactattaa aagacgcttt cgtggcagtt gaccaaatgg
    31081 aaaaatttgt tattttgaaa acattgccgg gaaatgcttc ggcagcagca aatttgattg
    31141 ataaacgtta caagaaagaa ttattttcga ttatcaatga tgacgataat gttttgatga
    31201 ttacacgaac tgaagaccag gcagttttat tgaagaaaga cttcctgcat tacctttaag
    31261 ttgaggagcg aatgtgatgc tacaagaaat cagtattact aactttgcga ttattccgga
    31321 attgcggctt tcttttcacg aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa
    31381 atccattatt atcgatgctt taggtctgtt agcaggagga agaggttcaa gcgattatat
    31441 acgtcaagga gcagaaaaat gtgtgcttga agggttgttt gaactaccaa aacaagaagg
    31501 attctctgaa ctgatggttg aattagggat cgaaacagat gaggacaatc tgattgttcg
    31561 acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt
    31621 agctaatctg agaagaatcg gcagttattt agtagatatc caaggacaaa acgaacatca
    31681 ggaactgctc cagcctgaat cacatcttgc tttattggat cgttttggag atgtagcttt
    31741 ccaacagaag aaaaagagtt atcagcaaga atatatctct tatcgtgagc tggaaaaaag
    31801 ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag agaatagata tgctccattt
    31861 ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga gaagaagaga aattaaaaga
    31921 agaaagagaa aaacttagca attatcaaaa aattgctgat ggattagctg caggttacgg
    31981 agcactgacg aataatgaac aaaacagtgt agatggtgtt ggcttggccg tttcagaact
    32041 gcaagggatc gcccatctag atgtggagta tgaagcaatt tatgaaaata tccaaagtgc
    32101 ttactatttg ctgcaagatg caatcggcga tatgagtcgt cagattgatt tattggagtt
    32161 agatgagagt cgtttagaag aagttacgca acgtttagaa ttaatccgac agttgaaacg
    32221 taaatacggc gaatccattg agtcaatatt ggcttactat gacgaaatca ccgaagagct
    32281 agcttcttct gatttttcag aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa
    32341 agagcttcta cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa
    32401 agaattggaa aaatccattc tgcatgaatt gaaaagtctg tatatggaaa acactgagtt
    32461 tgaggtacgt ttcttaaaag aagaaaatag acaattgaat catgatggat ttgaccaaat
    32521 cgaattttac attacgacca atccaggaga gccactgaag cctttggtga aagtcgcttc
    32581 tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact attttttctt ctgagcaagg
    32641 tgtgacaagt attatttttg atgaagtgga tacaggtgtg agcggaagag ttgcacaggc
    32701 catcgcagat aaaatttctc aaatatcgaa atactctcaa gttttgtgca tcactcatct
    32761 gcctcaagtt gctgcagtcg ccgactatca atattatatt gtgaaagcag tgattgatgg
    32821 acgtacgcaa acttctgttt cagagttgaa aacaaaagaa agagaagaag aaattgcacg
    32881 tatgctggct ggaagtgaga ttacaaaact cacactcgaa catgcaaaag aactattgaa
    32941 actcgcaaaa aggacctgag ccacctcgca gtggttcagg tccttttcag cagctgatag
    33001 ctatatttta tttttacatg acactcatta cagcagtcaa tacaacagat ccaatcgtta
    33061 aaatcagcat tgtccaaatg acaaattttg ttactttagc aaaagtactt gtttgttttt
    33121 tttcacgcat tgttttcaac ctcgtcatta attagttgat tcaagtgtac aatctttttt
    33181 ccatttccac aagagggctt ttttataaaa acttcccttt ttttaaataa taggtagtta
    33241 cgatacaaat aaataatgtt aatagaacta gccatataaa ggcatcatcc ctttcagcaa
    33301 taggtaactt aatgttcatt ccatagatac tgccgacgat tgtcggtata gttaaaataa
    33361 tcgttaaaga agttaaaatt ttcataatat tattcagatt attcgatacg attgctgaaa
    33421 aagtctgatt catttgttct actagtttta attgaatgcg agaagtagtc attgcctgct
    33481 ctgtttccac caatacatca cgtaaatgat ttttcaaagc atgattttct tggaaaagtt
    33541 tattttgcgc tagttctttc aatgcatcta aattagcaga agtcgcttct ctgaaataaa
    33601 cgaggctttt ttggatatcc atgatttgat aaagttgttt gttttcagtg gatacttgaa
    33661 gtttggcttc caaagtatcg cattgagatt gaagcgtagc taaataactg ttgtaagaga
    33721 gtgtgacctg ccaaatgaat tgaaagaaga tattgagatt aggcgaaata tcactttttg
    33781 gaaaggtctg gctaaagact ttctttaaaa acagtggttc atgattcgta attgtgataa
    33841 gcttcttttt gggcgtcaaa ataatagaga gcgggtatgt atccacttga aaataaccgc
    33901 taggacttgt tttagcaaaa ggatattgta acaataataa cgctggcttt gtcaactttg
    33961 attgattgaa ttcttctgta cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat
    34021 ctgacggaat ttcaaattga tgtgtcagct gatgaatctc ttcttgatta ggacgttcta
    34081 cacaatacca gatcgtttgg tcagtttcct ttccggtatt acgcagtttc tcattatcta
    34141 atgtaaaata tttgatcatg attttcctcc tgttgcctgt ttcttttatt ataacggata
    34201 gggaattgga atacatggaa tgtgtttgta agatatagaa tataactgca taaatttaaa
    34261 taaaatgaaa cagttgtgta aagtatatac agtatacttg aagcaacgat aaaaaaggag
    34321 tttacgttca tgggcacatt cacaccactt atcttgtttt ggtatattta cccggtaatc
    34381 gttttgtttg gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata
    34441 aaagcccctg atttggcgat tccttttttg tggggcggta tgcatgcatt gtctagaaat
    34501 acagggacaa tttctatctt accgtttttc tttatttctg ttttgctaat gggaatttgt
    34561 attgctgttt tccaagcata ttattatgaa gaaattattt atccgcgtta tttcaagatg
    34621 acttggcgtt tggtttttct gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac
    34681 atactttctt acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt
    34741 gcagcttgat ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg atttcccaga
    34801 aaaggtggta gaaagtgggg gattgtggta gactaatttt agttagtgga ggaatggggg
    34861 gctttcaatg ttgatgggtg aattccaaca taatattgat gcaaaaggac gtttaatcgt
    34921 gccctcaaaa cttcgtgaag aacttggcga aaagtttgtt ctgacacgcg gattggatgg
    34981 ctgtcttttc ggctatccga tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat
    35041 gccgttggct aaaaaagacg cacgtacttt tgtgcgattc ttctattcgg ctgccactga
    35101 atgcgaactt gataaacaag gacgaatcaa tatacccagc acgttaagaa attatgcagc
    35161 tttgacaaaa ggttgcgtag tcatcggtgt atctaaccga attgaaattt gggatgagac
    35221 acgatggcaa gatttttctg cagcagcaga agagaacttt gacgaaattg ctgaaagtat
    35281 gattgatttc ggactataag aataggaaga ggctgactaa tgactgaaac atttcaacac
    35341 tatacagtca tgttgaaaga aacggtagat ggattgaaca tcaaaccgga tggcgtttat
    35401 gtagactgta cactcggcgg tgccggacat agtgaatatt tattaagcca actaagttct
    35461 aaagggcatc tgtatgcgtt tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt
    35521 ttagcttctt atgtcgaaca aggtatggtc acatttatca aagcgaactt cagagaatta
    35581 gaagaacgct tgagtgagca tgtagataag gtagacggca ttttgtatga tctaggggtt
    35641 tcctctcctc agctagatga agcagaacgt ggatttagtt atcatcaaga tgcaccgttg
    35701 gatatgcgca tggatcagtc agctccttta tccgcttatc acgtaattaa tgaatattcc
    35761 taccatgaat tagtaaagat tttcttccgc tatggcgagg agaaattttc caaacaaatt
    35821 gctcgtgaaa tcgaacgagt acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc
    35881 gaaatcatta aacaagtcat cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa
    35941 agaatttttc aagctgtgcg gattgcagta aatgacgaac tcggtgccga agaggcctct
    36001 ttagaacagg caatccggtt attgaaagtc aatggaagaa tcagcgtaat cacattccat
    36061 tcattggaag acagaattgt caaaagtatg tttaaagaat atagtacggt acaagatcta
    36121 ccaccgggac ttcctgtcat acctgacgaa ttccaaccag aactgaaagt cattactcgt
    36181 aaaccaatcc ttccttcaga ggaagagcta acagaaaaca atcgatcacg cagtgcaaag
    36241 ttacgtattg cagaaaaaat aagaatgaaa gaggagtaga atgatggcag aattaaagaa
    36301 aatgcaagat tacgaatatg atttacctga attggtggat cagccggaac aacaaatgcc
    36361 tgattcttct tcgaatcaag aaatcattct tccacaatca ccggcaaaac gattaaagca
    36421 gatttctgct ttggaaaaaa ttgctgtggc aagcgtggcg attgctattg ttgctttatg
    36481 tgttttaaca gttatgctgc gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat
    36541 acaaacagaa atcaatgata aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga
    36601 actttctcgt actgaacgga ttaaacaaat cgcagaagaa aaaggattgt ctattaatga
    36661 cgacaattta aggaaagtga agtaaatgag tcttaaaaat aaaattcggc ggtttatgga
    36721 aaagaaaaat ctaaatccga tgaacaatcg caaaaaagta ggtatcattt tatttgctac
    36781 gagtattgga ttgttctttt tatttgccgt ccgtttctcg tatatcgtta ttggaggtca
    36841 tgttgctggt acctcattag ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt
    36901 aaaagcaaaa agaggaacga tttatgatcg taacggtgtg gcgctcgcag aagatgcctc
    36961 ctcttattca attaaagcga tactctcaaa aacatatacc tctggggata aaaaactgta
    37021 tgttgaagaa aagaattttg ataaaatagc agaaatcctg cataagaatc tttctataga
    37081 caaaaaagat gcactgaata ttttagagga cggcgcaaaa aaagagctgt atcaagttga
    37141 atttggtagc tatgggaaaa atatctccca agaaaccaag caaaacatcg aagcggacat
    37201 gaaaaaagaa ggtgtcgctg gtctttattt tgtcgaccat caggctcgga tgtatccaaa
    37261 tggcgtcttt tcttctcatt ttattggata cgcagttcca gacaaagatg agaatggctt
    37321 agttggtaaa ctaggtttgg agtctgctta caatgatatc ttgagcggta aagatggtaa
    37381 gattatttat caaaaagata attttcaaaa tcctctgcca ggtacagtgg cagaagaaga
    37441 aaaagctgtc gacggacaag atatctacac gactttagac agcagactac aaagttattt
    37501 agaaacactg atggatcaag taaatgaaga atatcagcca gaagaattga cagcagtcct
    37561 gatgaaagca aaaaccggag aaatcttggc aatgggacaa cgacctactt tcaatcctga
    37621 aacaatggaa ggattgactg gaaaagatgc tatctggcgt aatttcttgg tacaagatag
    37681 ttatgaacca ggatcaacca tgaaagtttt cactactgca gctgctatcg aagaaggaga
    37741 attcaacgag aatgaaacct tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa
    37801 tgaccatgac tttggtgaaa aaggtgtgct gaccatgcgt caagctcttt catggtcaag
    37861 taacgtcggg atggtcatac tggaacagcg attaggcgga cgatggtata actatttaca
    37921 aaaactggga tttggacaaa gtactcattc tggattagat gatgaagtaa acggtgcttt
    37981 acctacatta aatattgtcg accgagcaat gagtgcttat ggacaagcgg taggtgtgac
    38041 gaatttccaa atgatgaaag ctttcacttc tattgcgaat aacggtacaa tgattcaacc
    38101 tcgatatatc agcaaagtgg tggatcccca aactggagaa gagcgaacaa ctcaaacaga
    38161 agttctaggg caaccttttt caaaagaaac aacagaaaaa gtccgtgaat atatgagaga
    38221 tgttgtcgaa agcgaaaact acggtagtgc ttatggtgta tatagtgtac ctggatacaa
    38281 tgtatcggca aaaaacggaa cagcccaaat cgcttctgat acagggggat atcaaacagg
    38341 agatacagcc tacctgtatt cgattgtgga gatggttcct tctgaagatc cagactatgt
    38401 cttgtatcta acaatgaaac accctaagac atatgatcga atggcattgg ctaaaatcgc
    38461 aaatccattg atgaaacggg caatggattt taaagaaact gaagaagatt cagatacaga
    38521 aacaaaaaca gaaaaagtat ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc
    38581 agctgatgct caaaaaagtg gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca
    38641 aaaacaatct actgccaacg gtgatcagtt gatctctgga gaaaaactga tcctatatac
    38701 tggtggtgat aagctgatgc ctgacgtaac aggatggtca aaagcagata tcatgaagct
    38761 ggggaaaatc ctcggtattg aagttagttt tgatggagat ggttattgtg tcaaacaaga
    38821 acttgctcca tatgaaaaaa tcactgaaga caaacttagt tttactttag aagaataaga
    38881 agagaaatag gagagaaaag agcatggaat ggacacaagc attgattccc attgtcagca
    38941 gttgtgcaat gacaattgcc gcaatgccgc tatttattgg ttattttcaa atgaaaaagc
    39001 aaggtcaggc gattcgtgag gagggaccaa agtggcacaa tagcaaagcc ggaactccta
    39061 caatgggcgg cttagtgttt ctgatcggct ctattttgac aggaatttgg gttggagctt
    39121 ggcaaaaaca gctaactcct actcttttca tcttgctttt tgtattagcg ttatacggag
    39181 taatcggctt tcttgatgat tttataaaaa tattcaagaa aagaaacatg ggactgaact
    39241 cgaagcagaa attgattgga cagattctag ggggaatcat cttttatctt gtttaccgtt
    39301 ctgaaggtta tccaggtgta ttgaatttct tcggactaga tctaccttta ggaatcattt
    39361 atggtatttt cgctctcttc tggcttgttg gattttcaaa tgcagtaaat ctgacagatg
    39421 gcatcgatgg attagttgct ggcttaggaa gtatctcttt tgcagcttac ggtctaattg
    39481 cttggcatca gcatcagtat gatgtattaa tcatttgctt aagtgtttta ggaggtttac
    39541 tgggtttctt cgtctacaac agaaaacctg ccaagatttt tatgggagat gtcggttcat
    39601 tagcgttggg cggactttta gcggcaattt ctataatgct aaaccaagaa tggacattgc
    39661 ttttgattgg tttgatctat gttatggaaa cagcaagtgt gatgcttcaa gttacttcct
    39721 ttaagcttac aggaaaacga atttttaaaa tgtctccaat tcatcatcac tttgaaatgt
    39781 gcggatggtc ggagtggaag attgatacag ttttttggtt gatcagcatt gtgacttcgt
    39841 tgatcacatt atggatcgtt tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat
    39901 gaaaataaga aagtgcttgt cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta
    39961 ttacatgaat tgggcgcact tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg
    40021 gaagcacaag agctgctttc tttagggata aaagtaataa caggtagtca tccaatcgaa
    40081 ttgttggatg aagaattttc attgatggtc aaaaatccag ggattcccta cagtcatcct
    40141 ttagtagcaa aagcccaaga aatgggtatt ccggtaatta cggaagttga gctagcttac
    40201 gaagtagcgg aatgcccaat tattgggatt acaggaacga acggaaaaac aacgacaaca
    40261 acgatgacag gactactact gaatgcagga gcagatcaag gaattgcacg tttagcgggt
    40321 aatattgggt atccagcaag tggtgtagcg caagaagcaa aatcagaaga taaaattgtg
    40381 atggaattgt ctagcttcca actcatggga atcacagatt tccgccctca tatagcggtg
    40441 attaccaata tctatgaagc acatattgat tatcatggta caagaaaaga atatgtcaaa
    40501 gctaaatgga accttcaaaa aaacatgaca gaaaaagatt atctgatcct taactggaat
    40561 cagtcagaac tacaagaatt agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca
    40621 aaagaagtgt tggaagacgg tgtgtatgct gacgattact caatatacta taaaaaagaa
    40681 aaaataatgg aaatatctga acttggcgta ccaggtaagc ataatgtaga aaatgcacta
    40741 gctgctattt ctgtagcaaa attgtatggt atatcaaatg aagctatacg agaaacgttg
    40801 cattttttcc atggcgtacc acatcgtacg caatatgtag gagaaatcca aggcagaaaa
    40861 ttttataatg attcaaaagc aaccaatatt ttagcgacta aaatggctct aagtggattt
    40921 gaaacgtcta aggtcgtgtt attggctggc ggattagaca gaggaaatac atttgatgaa
    40981 ttaattcctt cattaaaagg aatcaaagca atggtcgtat ttggccaaac aaaagaaaag
    41041 ttgatggatg ctggaaaaaa agcaggcatc gagacaatag taacagccga ttctgtagaa
    41101 caagcagttc ctttagcatt agaaaacagt acagatggtg atgtagtttt attatcacca
    41161 gcgaatgcca gctgggatca atacccaaat tttgaaacta gaggaaacag attcatggaa
    41221 gctgtgaacc gattaaaata gaaaaagtag aagcgagcga taaaaaatga aaattttggt
    41281 tacaggcgga ggaacagggg gacatattta tccagctctt gcctttgtca attatgtaaa
    41341 gactcaagaa ccgaatgctg aattcatgta tgtgggagca aaacgaggtc tagaaaacaa
    41401 aatcgtccct gatacaggaa tgcctttcca taccttggag attcaaggat ttaaacgaaa
    41461 aatttccatg cataacatca agacggtccg attgtttctt aagagtatcc gtcaagcaaa
    41521 aaaaatcctc agtgattttc aaccagatat cgttatcggt actggaggat atgtttccgg
    41581 agcggtagtg tacgctgcag ccaaaatggg tattccaaca gttatccatg aacaaaacag
    41641 tgtaccaggt gttaccaata aatttttaag ccgatatgtg gatcggatcg ctctttcatt
    41701 taaagatgcg gcttcctttt ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc
    41761 acaagaagta gcagatacta aaaaatcaga tattttgaaa acatttgatt tggatccaga
    41821 aaagaaaaca gtcttgattt ttggcggtag tcaaggagca cttaaaatca atcaagcagt
    41881 atcagttttt ttaagtgaat atattccttc ttcttaccaa gttttgtatg cttcaggaga
    41941 tagatattat gaagagatcc taaataaaat aaatcctgaa atacaaaata ttagtgtcca
    42001 gccttatatc aaaaatatgg cagaggtcat ggcttccagt gatctgctgg tggggcgtgc
    42061 tggtgcaact tcgatcgcag aattcacagc tctggggtta ccggcagtgt tggttcccag
    42121 cccatatgtt acgaatgatc atcagactaa aaatgcaatg agtcttgttc acgcaggagc
    42181 agcaaaaatg attgcagata atgaactaac aggtgaatca ctaagtcaga cagtcaacga
    42241 aatcatgggt gatgaagaat tgcaaaagca aatgtgccga gcttctaaag aacaaggaat
    42301 ccctgatgct tcaaaacgtc tttatgactt agtaaaacaa ataatcaaaa aataaaggcg
    42361 gtggtcttag tgagtaagaa aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa
    42421 aacaatttga ctccttggca aaaggctaac cgaaaatatc tggcagaaca agagatgaaa
    42481 aaccaagagc caaaaaaaga agacataaca ttagatacac ccctttcaga ttctgaagaa
    42541 gaaaaaacgg ttcaagagga acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt
    42601 tttgaagaga tcaagcaaaa gaaaaacggt ggaccatata acggttcttt tttgaaccgg
    42661 ctgcccaatt taaaaagtca acggcacaaa gtactatttc gtcggttagc tttgatcatc
    42721 actgtattgg gtataccgct gatttttttg atctattatg tttctcctta tagcaaatta
    42781 caagcagttg cagtatcagg caataagaca gtcaacagcc aagaaatcat ctctgataca
    42841 aaattgtcat taggagaaaa tgtttgggga cagtattttc atcgatctac ttatatcgat
    42901 cgccttaaaa aagcacagcc aagaatcgaa acagcaaata tacatttcaa agggatgaat
    42961 gaattcgatt tggatgtaac agaatataaa gagattgctt tgatcgcaaa aaacaatcaa
    43021 tactacccag tgattgaaaa tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa
    43081 aatctcccta tcttggaaga gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa
    43141 tacaatcagt tatcatcaga attacaaaaa gcaatttctg aaatcaagta cacgccaaga
    43201 gcaagcaata aaaatctgat ccaattgaat atgaatgacg gcaatcaagt tattgtaaat
    43261 atcaataatc ttgcgaacca aatgaaatat tattcgcaag tagccaaaga tatggatgaa
    43321 aaaggtgtga tcgacatgga agttggtatc ttctcgcatc cttacggtag cactcaagaa
    43381 acggaagaat cgggccaatc ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca
    43441 aacgaatcaa gtgaagaggc agaaacgcaa aaagcacaaa cacctggatc gtctagcaca
    43501 aacggatcat ctgattcaaa tgattcaagt gaaacaagtc aaatagaaga taatccaagt
    43561 acaactccgt caagtttaaa tgaaaattga taaaacaact ttaaaaagta agaatgtact
    43621 ttctaaaaac cagtaattta tggtagaatt ggaagcagtg aatatatcta ttaataagta
    43681 tattttaaaa cggacgctag ttgttcgctt gtatatggaa tagtaagtag gagggaatcc
    43741 cattcatggc aaaaacagga atgtatgtag gccttgatat tggaacgaca tctgttaaag
    43801 ttgtcgtggc tgaatatatc gacagccaaa tgaatattat tggagtagga aacgcaaaat
    43861 cagaagggat taaccgaggc attatcgttg atatcgataa aacggtccaa gcgatacaac
    43921 gagcagtacg acaagcagaa gaaaaagcag gtatccagat caaaggcgta agtgtcggtt
    43981 tgccagcaaa catgcttgaa gtagaaaatt gccagggaat gattgcagtt aacggagatt
    44041 caaaggaaat caccgatgaa gatgtcagaa atgtggcttc agctgccctt gttcgttcaa
    44101 tacctcctga acgtcaaatc gtgtcaatat tgccacaaga ttttactgtc gatgggttcg
    44161 agggaatcaa agatcctcgt ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt
    44221 ttacaggacc taaaacgatc atccataaca tacgtaaatg cgtggaaaat gccggattaa
    44281 tcgtcaacga aatggtgatt actcctttag cgctgactga atcaattctt tctgacggag
    44341 aaaaagattt tggtacgatc gtcattgata tgggtggcgg ccaaacaaca acggctgtta
    44401 tgcatgataa gcaattgaag tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta
    44461 aagatatctc tattgtatta aatacgtcat ttaacaatgc agaagcgttg aaaataaact
    44521 acggggatgc atacccagaa cggacatcac cagatgaaga atttccagta gatgtgattg
    44581 gtcagtcaga accagtgaaa gtcgatgaac gctacttgtc agaaattatt tcagctcgaa
    44641 tggaacagat tttcaacaaa gcaaaagatg cattggacca aatcgaagca ttagagttgc
    44701 caggtggcgt cgtattgaca ggcggagctg ctagtcttcc tggtgttgtg gacttggcac
    44761 aagaaatctt cggcgtaaat gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc
    44821 cggtatttac aaatgtaata agtatcgtgg attattcagc aaacttgagt gaagtgtatc
    44881 aattggcaaa aattgctgtt acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac
    44941 aagaaacaac aaacacatat gagagctatg aagcacctga agaaacaatc tacgatgagc
    45001 cggaaccaaa agagtccgga gaaaatgtaa aaaataaaat caagggtttc tttacaaata
    45061 tttttgatta attcatcctg ggaggaataa gatatggaat tctcaattga taataatatc
    45121 aatgacggcg cagtaatcaa agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac
    45181 cgaatgattg aagaaaacgt taaaggtgtt gaattcatta cagccaacac agacgtgcaa
    45241 gcattaaaaa attcaaaagc tgaaacagtt atccaattag gccccaaata tactcgtggt
    45301 ttaggtgctg gttctcagcc agaagttgga caaaaagcgg cagaagaaag tgaacaatcc
    45361 ttacgcgaag ctttagacgg tgcagatatg attttcatca ctgcaggaat gggtggagga
    45421 accggtacag gtgctgcacc aatcgttgca ggtatcgcga aagaattggg cgcattgact
    45481 gttggtgtcg tgacacgtcc attcactttt gaaggaccga aacgtggccg ttttgctgca
    45541 gaaggtatcg ctcgtttgaa agaaaatgtg gatacattat taattatctc aaataaccgt
    45601 ttattagaag ttgtagataa aaagacaccg atgcttgaag ctttccgtga agcagataat
    45661 gttttacgac aaggtgtaca aggtatctct gatttgatca cagcgccagg ttacgttaac
    45721 cttgactttg ctgacgtgaa aacagtgatg gaaaaccaag gtacagcatt gatggggatc
    45781 ggtgtggcta gtggagaaga tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca
    45841 ttgcttgaaa catctattga tggagctgaa caagtacttc ttaacatcac aggcggtttg
    45901 gatatgacat tgttcgaagc acaagatgct tcagatatcg tagcaaatgc agcaactggc
    45961 gatgtaaata tcattctagg tacatctatc aatgaagaaa tgggcgatga aatccgtgtg
    46021 acggtcatcg caacaggtat cgatgaatca aaaaaggaac gcaagtctag ccgaccagct
    46081 agacaagcac aaatgcaatc acctgctcaa aagactgtat tagatatgga tcaagctaaa
    46141 ccaacttctt cagaagaaga aaacagtttt ggcgattggg atatcagacg tgaacaaaat
    46201 gttcgtccta gagttgatga ttccaatttt gacaatatcg aaaaaaaaga atttgataca
    46261 ttcaaccgcg aagaaacaaa atctcatggc gatgatgaat taaatacgcc accgtttttc
    46321 cgtcgtaaaa aataaaggag aggaagagca catgattgct gacaacttgc agaaaatcaa
    46381 gcaagaaatt caggattcgt gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt
    46441 agctgtaaca aaatctgttg aatccgctca agctgaagaa ttggttagag ctggcgttat
    46501 gaattgtgca gaaaaccgag tagataaatt attagaaaag aaacaagatt tatttgattt
    46561 tcctgaaata aagtggcatt taattggtaa tttacagcgc agaaaggtaa aattgattgt
    46621 aaatgaaatt gattactttc atgcgttgga cagtttaaga ttagcagaag aaatccagaa
    46681 gcggttagag aaagaattat cctgttttat tgaagtaaat gtttctggtg aggaatcaaa
    46741 acatggaatt tcaccagatg agcttttacc gtttgtggaa tctttagctg cctgcacaaa
    46801 aatcaaggtt gttgggttga tgactatggc gcctaaagaa gcttctgatg agcaaatcca
    46861 taaagtgttt ggtacgctga aagaattgcg tgatcttgtt caagcaaaag gttatgccca
    46921 tgcaccatgt agtgaattaa gtatggggat gagtcaagac tttcaaattg cgattgaaga
    46981 aggtgcaaca tttatccgtg taggaacagc gctattcaaa gacgaataaa ggaggaacaa
    47041 aaatgccgct tttaaacaga ggagcaattg ctaatttttt cggattagct gatgaggaag
    47101 agtacgaata ccaagattat cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa
    47161 atacaacaca gaatcaatca actcattctg tatctgctgc gaatactcat gaaaaacaaa
    47221 aaacggcgac ttcacgtcct gtaacgcatg aaagtattca gccggctgaa aagaaaaaag
    47281 taattgaact acatcaatca cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa
    47341 cctctacaca acctaaagca aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt
    47401 attctgaagc tatggcgatt gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt
    47461 ttcatttagt agaagaaaaa caagcgagaa gaatcgttga ttttctaaca ggaacagttt
    47521 atgcactaga tggagatatc aaacgtgttg gagatgagat ttttctttgt acaccgactg
    47581 ggatggagat tgacagttca accgctcaat cttttgcaga tgcgaatttg tttgatctgt
    47641 aagaggagga actacgattt atactattgc actattactt catttacttc aacgggcagc
    47701 ctatttctat agcattatct tgatggtata tgcattgctc tcttggtttc caggcgggta
    47761 tcaatcaggg ctaggacgat tattagctaa gatctgcgaa ccgtatatca gtttgttcga
    47821 tcgtctgcct ttgaggattg gacccttgga tttcacaatt ttaggggcaa tcctgatctt
    47881 gaacttagcc gtacaaggtt tagcacagat cgttatatgg ataactgtcc atttagttta
    47941 ttgattgaag caacaaaatc tttattctca gaatagatag gaggggaaaa ttttggatgc
    48001 aaatgtatac cagcactttc gggcagatga acgtacattt atcgattcgg taggcgattg
    48061 gattgaacag gttcaaggac aatatgcacc ctatctgacc gaatttcttg acccaagaca
    48121 atcctatatt ttagaaacac tgattcgaca aagcagtgat ttgcgtttca tgttttatgg
    48181 cggatatgaa caggcagaaa gaaaaagatg tttgattttc cccgattatt acgaacctgt
    48241 agaggaagat tttgaaacag cattgtatga aatccattat cctagcaaat ttgccagcct
    48301 aagtcatggg aaaatccttg gaactttagt gggaacgggg atcaaacgct cttactttgg
    48361 cgatatcatc tcggacggtg atcgctggca agtatttatt gcaaatgagg tggatcattt
    48421 tgttgctgca caagtaacaa agattggaaa ggttgctgta cgcttggaac caatcaaata
    48481 cacagaaatg attatgccga aagatagctg gacacaagaa agaggaacag ttagttccat
    48541 gcgactagac agtgtgatct ctgagattta taatatttcc agacagcggt ccaagcagct
    48601 gatcgaagct ggtaaagtaa aagtgaactg gacagaaaat acaagacctg attttgtact
    48661 tgaactatta gatattattt cagtcagagg ttatggcaga gtccagatcc aagaacttga
    48721 ggggaagacg aaaaaagaaa agtacagggt actgttcggt gtattacgta aataaaataa
    48781 aaaggtggtt tactcatggc tttaactcca ttagatatcc aaaataagac attcccgaca
    48841 aaaatgcggg gatataatca agacgaagta gatgatttct tagatttagt tgttcgtgac
    48901 tatgaggaat tgactcaaag aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa
    48961 aaacttgagt acttcaatga attaaaagat gctttgaacc aatcaatcat cgtagctcaa
    49021 gatacggctg ataaagtaaa aacaagtgca agcaaggaat ccgaagtgat cgttacttct
    49081 gcacaaaaca aagcagatga gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca
    49141 actgatgcag aagaaaaagc aagaaaaatc ttgacagatg caacagaaaa agcaagacag
    49201 cttgcgacag aaactgaaga tcttaagaaa aaaacacgag tattccatca acgtatcagc
    49261 ttgatgcttg agtctcaatt agaacaagtg aaaagtccgg aatgggatga aattttacaa
    49321 ccattctcca gctacgtgac tgacagccat gaagtgatca aagaagtttt agcaaaacag
    49381 cttgacaatg aaaatgatac tgatgtaaac tcagacacag gagtcataga agcacctatg
    49441 acggcatttg atacacaggc tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag
    49501 tagaacagaa aagcactttg ttattcttaa agcgagccga tgagagtgga agttcggtaa
    49561 gacactgcat ggtgaaagat cctctacttg agacctgctt ttccgaaatt agagtaagaa
    49621 gcagcggctg acctcgttat tggtcacaga gagtttgcac tagatagatg caactaaatt
    49681 tgggtggtac cacggcatgt tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa
    49741 acaaataaag gagtgtatgg ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca
    49801 aaattcccta tgcgtggtaa tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa
    49861 gaaaatcgaa tctaccaaaa aagacaagag ttgaacgaag gaaaacctag ctttgttctt
    49921 catgatggac ctccatatgc caatggtaat atccatttag gacattcatt gaataaaatc
    49981 agtaaagaca ttatcattcg ttcaaaatca atgtctggct atcgtgcacc atatgttcca
    50041 ggatgggata cacatggatt gccgattgaa caagtattgg ctaataaagg cgtgaaacgt
    50101 aaagaaatgt ccatggcaga atacttggaa aaatgtcgtg aatatgcact ttcccaagta
    50161 gataagcaac gagcagattt caaacgccta ggagttgcag gagattggga acatccttat
    50221 gttacattag atccttctta tgaagctgca caagtacgcg tatttggtaa aatggctgaa
    50281 aaaggctata tttacaaagg attgaaacca atttactggt ctccgtcaag tgagtcttct
    50341 ttagcggaag ctgaaattga atataaagat gtaaaatctc catctatttt tgtagctttc
    50401 aatgtcaaag acggcaaaga tattcttgat gaagatacag cttttgtcat ttggacgaca
    50461 acaccatgga cattgccagc aaaccaaggt attgctgtaa atccaaaatt tacgtatgta
    50521 ttggtggaag ccgatggccg taagtttgtc gtagcgaaag atttgctaga aacagtccaa
    50581 tcagaaatcg gttgggaaaa tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa
    50641 tatatgactg caagtcatcc attctatgaa cgtgaatcac ttgtgatttt aggcgatcat
    50701 gttactttag acgctggtac tggattagtc catactgctc caggacatgg ggaagatgac
    50761 tatatcgctg gtagcaaata taaactatct gtagtgtcac ctgtagactc aaaaggattg
    50821 tttacagaag aagcacctgg atttgaaggt atcttttatg acaaagcaaa tcctatgatc
    50881 actgatctat taaaagaaaa aggagctttg ctgaagctag atttctttac tcatagttat
    50941 cctcacgatt ggcggacgaa aaagcctgtg atctatcggg caactccaca atggtttgca
    51001 tctatcgata aattccgtca gaatattttg gatgaagtag aaaaagtaga gtgggttatc
    51061 ccatggggta aaacacgttt gtataatatg atccgtgacc gcggcgattg ggtaatttcc
    51121 cgtcagcgtg catggggagt accacttccg attttctatg ctgaaaatgg agaagcaatc
    51181 atcacaccag aaacaatcga acatgtggca aaattatttg ctgaacatgg atcaattatc
    51241 tggtttgaaa aagaagcgaa agaacttctt cctgaaggat ttacccaccc aggttctcct
    51301 aatggcgaat ttacaaaaga aaaagatatc atggatgtct ggtttgattc tggttcttct
    51361 catgaagctg ttttacgtca gcgaccagaa ttatcattcc cagcagatat gtatttagaa
    51421 ggatcagacc aatatcgtgg ctggttcaac tcaagtatca cgactagtgt ggctatcaat
    51481 ggtgtagcac catacaaatc agttatttct caaggatttg ctttagatgg tgaaggccgt
    51541 aagatgagta aatctttagg aaacatcatt gcaccagaaa aagtgatcaa acaatttggt
    51601 gcagatatcc ttcgcttatg ggtatctagt gtagattatg aagcagatgt tcgtgtttct
    51661 atggatatct taagtcaagt ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc
    51721 ttattagcga atacagaaga tttcgatcct aaaaaagata ctgtttcgta caatgattta
    51781 cgttctgtag acaaatatat gaccgttcga ttgaaccaag taatcaaaga aatccgagaa
    51841 gaagggtacg acaaatataa cttcatgcat atttaccgta cagttatgaa cttcttgacg
    51901 gttgatctat cgtcattcta cctggatttt gctaaagatg tagtttatat tgaagcagaa
    51961 gatgattatc aacgtcgttg catgcaaact gttttctatc aaacagctgt agctttgaca
    52021 aaattattga ctcctatcat tccgcataca gcagaagaaa tctggtcatt cctgaaagaa
    52081 gaagaggagt acgttcagtt aagcgagttt cctggttatg aagagtttgc aaaccaagaa
    52141 gaattgatgg atacctggac tgctttcatg gacttccgtg ataatgtctt aaaagcattg
    52201 gaagaagcaa gaaacagcaa attgattggt aaatctttgg aagcaaaact aactgtttat
    52261 ccaaaacaac aagttcgtga aatgttaaaa gctttggatg cagatattgc acaattattg
    52321 atcgtttcac cagactactt tgaagttatg gcagcagatg aacaagtgcc agataacgct
    52381 atggtatttg atgatgttgc tattacagtg gaaaaagcag acggggaaac ttgcgatcgc
    52441 tgtcgccaag ttcgtaaaga tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt
    52501 tgtgcaaaaa tcgttgaaga cttctatcct gaagctgtag ctgaaggatt cgaagaaaag
    52561 taaaagggaa aagggggtgt gacatgttct ttgtcacacc cccttttgtt atcaatcgtt
    52621 taattttcca cgttcacgat accagttatc tggttgccag atccattcga atccgtcttt
    52681 ttcaagtagt tcgaaagctg ctttaggtcc cattgatcca gctttgtagt tgggaaaatc
    52741 cggttgtgtt tggtcccaag cttttcgaat gacatccacg atatgccaag attgagcgac
    52801 ttcttcccaa tgagagaagt tcgttccatc accattcaac gcatctaata gtaatttttc
    52861 gtaagcttct gggctatttt caaccatttc tgcactattt ctaaattcca atttgattgg
    52921 ttccgtttca aatccttgcc ctacttcttt cccattcaag gataaagaaa atccttcagt
    52981 tggctgaata tagatcgtta gaacattagg tggaagtgtc gtatcgtcac atggttcatc
    53041 gacagatgtt ttgaatacat taacaggaac ttgtttgaat acgatgttga tccgagtccc
    53101 tttttcagtc aaacgtttgc ctgtacgtac atagaaagga acacctgacc aacggaagtt
    53161 atcgatagtg aatttgcctg cgacgaatgt ttcagtctct gattgagcat ccactttgtc
    53221 ttcttcgcga tagcccttga attgtacacc atccaattca ccttgagcat attgcccgcg
    53281 aacgaagttt tctaacactt cttcttcgtt ataaatgcgg atagctttca atgctttgat
    53341 cttttctgta cggatttctt tttcggaaaa tgctactggc ggttccattg caagtaatgc
    53401 tacaacctgt aaaatatgat tttgcaccat gtctttaagt gcgccactat gatcgtagta
    53461 gcccccgcga tcttctacac cgaggttttc gccaaaagta atttggatat tgtcgatata
    53521 gcgattgttc cattgagcct caaaaatatt attggcaaaa cggatagctg agatgttttg
    53581 gatcatttct tttcctaaat aatgatcaat cctgaaaata tcttgttctg ggaatacttc
    53641 tctgatttgc tggttcaatt caaaagcaga ctggtaatca gaaccgaacg gtttttctat
    53701 gatcaaacga ttgaagccat tttttgtcat aatattttgt gatttcaaat gagaaacgat
    53761 cgttccaaaa aattgaggtg ccatagccaa ataatagaca cggttaccgt ttaattgata
    53821 gtgtccatct aatcggtcag acaattcttt taatgtctga taatgcgttg aatcatttac
    53881 atcatgggat tgatagtaaa aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt
    53941 caatgcacgg attgtttctt tgacaacttc acggtagtgc tcatcgctcc agggacggcg
    54001 agcggttccg attacagcaa aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa
    54061 agacgggtac aatttacgct gggctaggtc acctgtaccg ccgaatatcg taaacaatac
    54121 atttttttca ttagacatgt ggatacactc ctttatattt tatcgacaaa aatcgtgctg
    54181 gccgccttaa agctgacggg gattttcttt ctatcatttt ggatagtgat cggtccttca
    54241 taagctgcga tgtcaacgat ctcatattct tcgttgatat tcaggtcaat agtgactaag
    54301 taatccaaca attctttttc atcaagcact cgtgcgatac gcacttttgt accgacagga
    54361 taggactcca atgtttgtcg gcgttcttca tggacaactt cctctttttt aggaatcgat
    54421 cctccatgag gacaatgttc aggatgattc aaatagtttt ctaaatgatc tgcaagatgt
    54481 tccgaagtca catgttccaa tacttcagca tcatcatgga cttcattcca gctatagtct
    54541 aaatgttcaa ctaaaaaaac ttcccataag cgatgtttgc gaatcagact gctagctttt
    54601 tgcagtccgc ttgcagtcaa ttgtacacct tgataagggg aatgttctac aagctcttct
    54661 ttcacaagtt tagaaatcat ttcactgaca gatgcagctg agacatctag actagatacg
    54721 atttgtttat tgttgacttt cgttgtatct ccacctagtt ctaaaatgat tttcaaataa
    54781 tcttctcgat ttggtgtcat attcgatcat cctttcatgg taaaaaacca tcattagtaa
    54841 gtgtaacaaa aaaaacgtta attgactatt tttctaaata aaaaacaaag gatttttaga
    54901 cgtttttaca acaaaaaacg aaagatgata gagaagactc attctttcgt ttataaagga
    54961 tatatatata cattgggagg gatctatttt tcagataaca acttatatga agtgtctgca
    55021 gattcgtcat aaagagtttg ttcgggataa gaaaagataa gataaaaacc atactgatca
    55081 cgtacgacta aacgatattc atctatttct accacagatg tgccaagaga ctgaccgttg
    55141 atcaaaaacg tgtaatcaga tgtaatctgt aattgttgtt tttgctggct atcactaaag
    55201 aaccatgtac cttgatactt tgagagtcct tctgactttt ccgcttgttt tttctttttc
    55261 aagtaaataa aacttgagag aacggtaatg agtgaaccaa gaaaatagat catttttttt
    55321 atcaccaaat cactccttta tgagaaaata atcgcataga catattcttt cttctttatt
    55381 tcattgtacc gaataaaagc taaaatttct ctaagacttt cgactggttt ttttactgtt
    55441 tttctacaaa ttatgatagg atacatagta ggaagaaagg tgacataatt tgaagtatca
    55501 gcaagtggaa atagcttttt ttattgaccg cccgaatcgg tttatagcac attgtatgaa
    55561 aaaagacgga gaaataatta ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct
    55621 gataccaggc gctgaagtgg ctattgtatt tgctcctgga cctaaacgga aaaccgccta
    55681 tgatctgatt gctgtaaaaa agaagggtga ctggtttaat atcgatagtc aattgcctaa
    55741 tcggctggca attgatggta ttttagacgg cacgatcctt ttaccaggat taactggtcc
    55801 atttacggta tacaagcgtg aagtaactta tcttcactcc aaatttgata tttatttaga
    55861 gacggtggtc ggacaaaaag catttgttga agtcaaaggt atgacacttg aaaataaagc
    55921 gattggtgct tttcctgatg cgcccacact cagaggattg aaacatatag gagaattaac
    55981 gtatgccgca caagatggtt atgccgctta tgtcttgttt attgctcagt tcgaacatct
    56041 tcatctggca acgatccatg aagaaatgca accagcacta gctgatatgg ttcgacatgc
    56101 gcagcaaagc ggagttcaaa tcctcgccta taattgtcaa gtatcggcag atgaggtaac
    56161 tgtcaagcag gccattccat ttgatgtaga tatccctttt gaagatccta tacaatagaa
    56221 agaaggtaaa ataatgatca acttaggtat tatcggaaca aattggatta ctcatcaatt
    56281 tgtccaagca gctttaagca caaaaaaata tgaattgaca gcggtatatt ctagacattt
    56341 ggaaaaagca caggaatttg gttctcatta tgaaggggat atcgaattcg caacagattt
    56401 gaagactttt ttcaatatta ctcatatgga caccatttat attgcttctc cgaatagtct
    56461 tcactttagt caagctaagc aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc
    56521 agctttttcg acgccaaaag aaatggatga aatcatccat ttggcaaatg aacacaatgt
    56581 gtattttttt gaagcagcaa gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta
    56641 cttaccatta aaagacgaga taatcggtgc aaatttcagc tatatgaaat attcttcgcg
    56701 ctatgaccaa gtattagatg gagaagaacc gaatatcttc tcgactcatt tctcaggtgg
    56761 tgcattgatg gatttaggtg tttaccttgt atatgcagct gttgcttggt ttggaatgcc
    56821 aaaagaagtc cattatttcc caagaaagat ttctactggt gtagatggtc ttggatgggg
    56881 gatcttacgt tatgagaatt ttgatgtatc gatccagcca ggtaaaatcg gtgattctta
    56941 tttaccgtca gagatttatt ttgactcagg aacgctagtg atgaatggag tcaatgcaat
    57001 tgaacaagca caatttcatg atcgcgcgca taaagaaatc attgatttgg cggtaacagc
    57061 tgcagaaaat ccaatggcag atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa
    57121 agatccagct tggggattgt tgtatgaaga gtggatcgag ctagctagaa atgtgaacca
    57181 agtgatttat gatttaagaa aaaatggcgg catcaaattc gatgcagata atgaggaaga
    57241 tttttaatga acgaatttgt acaagtattt cccgaaaatt ggcagaaaaa atatgaggat
    57301 aaagggttta tctcaccttc tcttattcaa cgagcagttt ttcagccact aagtaataaa
    57361 caaagtatag tagcggtttc tcctacaggt tcaggtaaaa cactggctta tctttggcct
    57421 ctactactaa acgtggaacc aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa
    57481 gaactagcta ttcaagtagc agatgttgca cgtgaatggg gaaaagacaa agagttgaaa
    57541 gtccaatctt tggtgggcgg agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa
    57601 ccagaaatct tgattggtac accaggaaga atattggaat tgatgaaagc aaaaaaaata
    57661 aaagcacatc aagtgaaaac aatggtattt gatgaagcag accagctatt tgacgctggg
    57721 aacagccaaa tcattgatca aattcttcat caagctccaa cagagtatca gctagctttt
    57781 ttttcagcga cagcagatcg ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt
    57841 gagacaattg acgtgacagc agaggacgat tctcgaaaag gactgagaca ttacttttta
    57901 cgtgtaccga tacgaaagaa agatgactac ttgagacgtc tcacgcatat agaagatttt
    57961 catggtctga ttttctttaa tcaactgact gaattaggcg taatggaaga aaaattgctg
    58021 tatagaggtg taccggtagg aagtctggcc tctgatcaga acaagctact ccgaaaagca
    58081 gcgctagaac aattcaaaaa gaaaatggtc agagcacttt tgaccacaga tgtggcggct
    58141 agaggtttag atattacagg tcttccttat gtagtgaatg cagaagttcc gttaagtgaa
    58201 gaagcttatt tgcatcgttc gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt
    58261 acactcgtac aagataataa tctaagagat ttaaaacggc ttttacgtcc atcaaatata
    58321 acacttgaag agattttttt atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa
    58381 gatgcagctc ctgcagtaaa taaaaagtat gcgaaaactt tttctggaaa ggagcagaac
    58441 ccagaatata acaaagaaaa aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga
    58501 aataaaggag caaggcgcaa ataagcgcct ttttatgcta tgattatgaa aacgggaaca
    58561 aaattggttg ccagaggagg ggttatgtga aagaacgtca agaagagatc ttgcagctat
    58621 tgacagaaaa ccaacaagga ttgacagcaa gtgatgtagc tgagcgctta actatcgatc
    58681 gaagcaatgc aagccgatat ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg
    58741 ctggccgacc agtcgtttac tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg
    58801 tcgactcttc tacgcaagtg acttttgaaa cactcgttgg agaaaatgat tctttaaaag
    58861 tcagcataca gcaggcaaaa gctgcgattc tttatccacc gcgggggttg catacgatta
    58921 tttttggaga aactggtaca gggaagtcga tgtttgctga atgtatgtat cattttgcaa
    58981 tcgattcaga gatgctttct gcagatgcgc catttgtttc tttcaattgt gcggattatg
    59041 atcaaaatcc gcaactgctc tttggacaca tctttgggat caaaaaaggg gcttacacag
    59101 gagcagctca agatagtccc ggtctaatag ccaaagcaga cggcggtata ttgtttttag
    59161 atgagatcca ccgtttaccg ccggaagggc aggaaatgct gtttactttc attgataaag
    59221 gtatttaccg tccgcttgga gaaagtgcac aagtacatga agcatctgtt cagatcattg
    59281 gggcgacgac tgaatcttca gaaagtttct tatctacgtt caatcgacgg attcctatgg
    59341 caatcacctt gccgaatctt gcttcacgtt cactggatga gcgttatgaa atcatctctc
    59401 tttttatcaa acaagaagcg aatcgtttga atcaacgtat cgatatcgaa aaagaagcga
    59461 ttcttgcctt catgctttat gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga
    59521 aactagtctg tgcaaaatct tttttacatt accgtactca tcatgagaaa aagctaatca
    59581 ttcgaaaaga agaactttcc cttcaagtac aaaaaggatt actgaaaatc aaggaaatac
    59641 cggaacgtct cgaccgattc atggacagca aaagtcagta tttgacattt gaaccaggat
    59701 ttgctgatgt tgtctggtca caagatccag aacgaaacat gcaggtctat aacgatattg
    59761 aggaaaaagt cacttctctt tccgaaaccg gtgtggagaa cattgatcta gagaccctca
    59821 tttcaaaaga tgtggatgct tatttccaga catatgtcaa agaattgact aaaagttcga
    59881 ttcccaaaga tcttttgcca gatgaaatct ggcggttaac caatcaattg tatgatgtgg
    59941 ctgaaaaacg gttagaccgt atttataatg aaaaagctcg ttttgctttt gctttgcatc
    60001 tgcaaagtac attggatcgt gtgagagaag gtcatatgat cgtccaccca gatttgaata
    60061 acgtacggaa aaattttaag agggaattcc aggtagcaat cgatctttca acgatcattg
    60121 aagaagaaga acaagttgag attccttttg atgaaatcgg ctttattagt atgtttcttt
    60181 ctatcaatct aggagaaagt gagcctgtta aagaaaacaa agtagatgtt ctcatattga
    60241 tgcacggcag acagactgct tcaagcatgt tggagatggc acaagaacta ttaggaacaa
    60301 aagtagggaa agcttttaat atgccactgg ttatggaagt tcaaacaatg tatgaagaag
    60361 tgttacaata tgtcaaaaac tatcaagagc atttgacgaa tggcctactc ttgttaacag
    60421 atatgggatc attaaacacc ttcagtgatt tgatttatga agagactggc attcggacga
    60481 aagctatcac gatgacaagt accatgattg tgatagaagc gatacggatg gcaagtgttg
    60541 gaagaagctt agaagatatt tatcaaaata tccagctttc ctttgaatcg atcgtacgtg
    60601 atcagttcaa agcggaaaag tctgaaactt tacagcgtaa aaaagccgta atcgtgacat
    60661 gctttactgg tgaaggagta gctgccaaat tatatcagcg tattatgcca gtgattaatc
    60721 aaaataaggt agaactgatc cagatgcagt ttttagaacg tgaatcattc aaaaaacaca
    60781 tcgatgcctt attggaagaa tatgagatca aagcaattgc gggaacagtt gatgttgatt
    60841 atcaaaatat cccatttttt tcagcctatg acgtttttga tgatgaaaaa ctaaatattt
    60901 taaagcgtat agctgatgat gagatcccga cagctaaaat tgtcgattca cttgctggat
    60961 cattaagcga tgtcttttct attgatgaac tgatccgttc attgcaaaag acagttcagc
    61021 agatccagac cgatttgtcg attatcgtgg aacctggagt agatactgga atcgttatcc
    61081 atttagcctt catgttagac ggattgatca aacaagaaag ccatcggcaa tttgagaaac
    61141 tgtctatttt ctcaaaaatt tatcgtttgg aaatggatat cgttcgggca aacttgatgg
    61201 cactagaacg aaaatatcag gtaaaaattc cagaagatga tatcgcattt cttacacaaa
    61261 tgtttcttga aaataaaata aaaaaatgaa cacactaatc atactgttta cacactaaac
    61321 atctgtgtgc gcactataaa aaagagctaa aaagcatgaa aacgctattt cagctctttt
    61381 ttttattggc atgcttcttg cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa
    61441 atggcaaaaa aaacaatcat gttagtttgt tcagcaggga tgagtacgag tttattggtc
    61501 acaaaaatgc aaaaagcagc agaagaaaaa gggatggaag ccgatatttt tgcggtctct
    61561 gcctcagatg cagataataa tttagaatct aagaatgtcg atgtattatt attaggtcct
    61621 caagtacgtt tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg tattccatta
    61681 gatgtcatca atatgcaaga ctatggcatg atgaacggag aaaaagttct tgcacaagca
    61741 gaaaatctaa tgaaataaat caagtatgca gttgggggta taacaacgtg gaagatcaac
    61801 aaaatctaga agccatcatg gggttaatca tgtttggcgg taatgcaaag agcgatgcaa
    61861 tggaagcgat tgcggctgct aaaatcggtg attttgagtt agcggatagg aaaatcgctg
    61921 atgcagaaga atcacttata caagcgcatc atgcacaaac agacatgttg acacaagaag
    61981 cacaaggaaa tcatatgcaa gtgacattat tgactgttca cagtcaagat cacttaatga
    62041 catctattgc gtttactgat ttagcaaaag aaatcatcga actttatcgt cgcgtataca
    62101 gcgaataaat acaacttctt ttttgaacgg aagaagaaaa atttttgaac ggatttttta
    62161 ttttgttata aggagaactc tctttatata aaaaacttat aggaggaagt aaaaaatgaa
    62221 cggactatca agttggatgg agaaatacat cctgccggtt gccgcaaaaa tcggagcgca
    62281 aaaacattta gttgctttgc gtgatgcttt tatcggcata ctgcccgcaa caatggcggg
    62341 atcagtcgct gtaatgatca atgcaattat tcgtgattta ccgcaacagt ttaacagtac
    62401 ctatgctgac agtttagcag cagacaaagg aatctttgcc gtattgaatg tcatcatcgg
    62461 aatcaatggt tttgtctgga acggtacact agcaatcgcc ggattgatct ttgccttttc
    62521 ttgggggtat aatcttgccc gagcttatgg agtaaatgat ctagctgggg ggatcgttgg
    62581 tttagctacc ttgattcaag gtatttcctt tggtccagca ttatcagcag cattagatgt
    62641 gaatgttcct tcaaatgtcg cagatgcaat caatagttct gagattggtg caacgattgc
    62701 agatggcaca ctgtcagttg gattatgggg ttggctaaaa ctagatcatt tgaatggaaa
    62761 cgcttatttt acagttatga tcatgggagc tatttcagtc attatttttt gtaaattgat
    62821 gcttgccaac attacgatca aaatgcctga ttcagtacca ccagcagtat ctaaagcatt
    62881 tgcagccatt ttaccagcga cgatcgcttt atacgtcatt gctattatca attatatcgt
    62941 aggtcgtgta gctgatgggc aactgatcat tgatttggtg caaaaatata ttgcagaacc
    63001 attcctagga ttatctcaag ggttgggagc agtattgatc gtcacagtat ttgttcaagt
    63061 attctggttc tttggtattc atggatcaaa tgtattggca ccagtgctag agggaatctg
    63121 gggacaagca caactaatca atattgatat tttccaaaaa ggttataacg gaaaaactgg
    63181 tacaccagcc gtgttagatg caatcgaaga tggaaaagct tatatgtggg tccgcggatc
    63241 gttcgatgct tttgcatggt ttggcggatc aggcggaacg atcgtcttac tgatagctat
    63301 cttgattttc tctaaacgtg cagattatct aacagtagct aaattatcca ttgggcctgg
    63361 tatcttcaat atcaacgaac cagtgatgtt tggtctgcca atcgtattga atgcaatcat
    63421 gtttatccca ttcttgattg ctccagtagt agcaacagca attggatgga cagctactta
    63481 tcttggccta gtcgctccgg tatcgcagca agtcacatgg gtagtaccgc cgttcttatt
    63541 gtcattctta gcaacaggag cggattggcg ggcaccgatc gtaacattag tatgtatggt
    63601 cgtgactttc ttgatttggg caccatttgt catctctgca aacaaaatgg accctgccgc
    63661 aggcgaacaa caataagatg aacgtgagga gcgggacgct ttaagtttcg ttcctcataa
    63721 tgaattttcg aggaggaact gaaatgatta caattgccag tgcaatgcaa caagatgaaa
    63781 tcaaagaatt gttagaagga tacaatgaag aaggaatgac ttttacattt aaagaaaaac
    63841 aaggaatcaa gctgttcttt gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag
    63901 caaaggaact aatcaaagca cagccttggg gaacagtcct ttattttcaa tcaacagctg
    63961 tttagaaaat ggtttaaaaa gataggatgg ttgagatgat aaaaaacggt ttatttttat
    64021 gtagtattgg ttctttgatc gtgttgtata gtttgaatcc acattcgcaa aggtatgatt
    64081 tgtatagtat ggtcacaggt ctttttctaa caggattagg aatctatttc ttcatgaagg
    64141 ggaaaaaaag agaagaagca aaggagaaaa ataactaatg gaaatcacga aaaaattaaa
    64201 tattccagca tcgttttttt acgataaagt tatggactct gtccttttcg atgtacgtaa
    64261 agcgaccggc aaatcagtaa ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt
    64321 ctcgcaaaat aaccgggctc gcataaaaat cgaagaagtc attgaaaacc agtcttataa
    64381 gtttcgtaca tcgacaacga agaatgaatt cgtcgttcat tatcaaatca aaccattgga
    64441 cgattcgacc tgtgaggttc attacacaga aaaaatggag tcttatggca tgcttcaaaa
    64501 gttgaatgat atgcttttag gaacaatctt gatgtatttt aaacggagac gtttcaaaaa
    64561 aatgctagat atgatcgaag caacgtatta aatcaatgaa acatggtact atttagttat
    64621 tgaaaaatga agtaagctag aaggaatttt tcataggaag atagatcttt cctgcgaatc
    64681 ctcactagct tttttgtagg aaggggcagc aaaatgacaa acaaagtaag agagctaggt
    64741 gtatcttcga ttgaggaaat gtttgattta gccacttatg catttaatgg ggcagataca
    64801 cctgaaaggc gagaacgttt tcgtacactt gcggagaatt cttggaatta cggatttttt
    64861 gatgaggagg atcggctaac aagtcaagtc atggcgaccc cttttccagt gaatttttac
    64921 ggacaagagt acctaatggc cggcattggc tatgttgctt cttatccaga agcaagggga
    64981 caaggcggga tcaatcagat catggaaaag atccttgaag attgccgtga tcgtaaagta
    65041 agcttatctt atctggcgcc attctcctat cccttctatc ggcgctatgg ctatgaacaa
    65101 tcatttgata aaatcagtta tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa
    65161 tctggaaaaa taaagcgtat ggattttgaa gatgcaaaag ctgcaatcag aagaatttat
    65221 gaacagatgc cggaaaacca acgagctggt ctgaagcgcg aagactggtg gtatacgtat
    65281 aaattcaagc aaaaaggcaa ttaccagttt gctttgcatt ttgatgataa tgaggaagca
    65341 acgggatata tcgtttatca gcttgcaacg tcttccttta ttattgcgga gtggggattt
    65401 ttggatcatg atgctttttg cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt
    65461 gaaacatttg aatattcatg cggctacggc ggtaatgatc aaaattatct attagaaaat
    65521 ccatttgctt ctgtaatgat cacaccttat atgatggcga ggatcgtaga tatttctttg
    65581 tttttggaaa attatccatt taaaaagaga caagctgcat ttgccctaca aatagaagaa
    65641 gatcattatg cgaaatggaa tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat
    65701 catctctcaa aagtagaaca aacaaaactc ccaatcgttc gaggaagtat ccaacatatc
    65761 acgcagctat tattagggta tcgtaaagtg gaagagttgg tattccaaga aagaatacag
    65821 gtgacaaaag aatatgaaga aacgttaagc tcaatcttgc cacagcaaca gccaattctt
    65881 aatgactatt tttgataaaa aacgggtgat gacaatattt ttgtcaccac ccttttgttt
    65941 ttcatttggc atttgaagga aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt
    66001 ttttgttttc ttttgtttta gggttttctt ttttgtggtc ttggattgct tctttcgtaa
    66061 tcttttcccg atttttttta gatgaatcta agcgttctgc gtatactttt tcttttggct
    66121 tatcattcat tggaaaggac ctccctacat tatttcattc tacctctagt ttatcatctg
    66181 ttaaggacta gtggaaacgt tttgcctaac ttataagctg atttttttag cagataagct
    66241 tgcaagtaaa aaacaaacaa gctacaattg atttattgaa attgttcctg tagctcagta
    66301 ggatagagca atcgcctcct aagcgatagg tcgctggttc aattccagtc aggaacgtat
    66361 aaataacccc tgtatccttt gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg
    66421 ccaaactagt tatcattttt atataaacta tcgaacacag tattgatttt ttctttatcc
    66481 tgatcttcaa gctctcgaac aatatgaaga taagtagaca ttgtagtttc taaactgcta
    66541 tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa aatagatgca
    66601 tgtgtatgcc ttagtccatg caaagtaaat tgcttttcaa tccctaattc ttttaatttt
    66661 ttgcgcaaat atttgctgac ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa
    66721 acaaaattat gagggttttt gatttcaaaa ttctcgaata attctttttg attgatttta
    66781 aactttttta agagatcaat cgtatgttta tcaatggaaa ttttcctatt tgaagtttcg
    66841 ttttttgttt ttccccattc atttaattta taattccatg ttttattaat agtgatgatt
    66901 tgatcttcga aatcgatatc ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca
    66961 gtggcaccag caactaacac gagcatagga gaagagtact gcgcagaaag gttttcttct
    67021 acgagtttca ttaaagattt aaattgatca taatctaaat atttatcttc ttttggcttc
    67081 aatgaatctt ttcctttgat tactgctttt ctagttggat caaacggaat taatccttct
    67141 tctacggcat ctttcaacga cgctctaata tggttattaa acttaataac ggtagatttt
    67201 acatggtctt tagcatactt atttaaaaag cgttgatatc ccactctatc taaatcagaa
    67261 atcaataccg ctggcatata tttctttatg ttcattaacg tatcttcgta ttttctataa
    67321 gtgataggag atactgttcc ttctttataa agttgcatcc aatcctcaaa gtaatctgat
    67381 aataatagat ttttgcgatc ccctttaagg cctttgttca agttatattc taattcgttg
    67441 gcggcatctt ttgcttctcc ttttgtctta aatcctgatt ttcttatctt cgtgtatttt
    67501 ccgtcatctt ttttgtatga aatttcgtat tgccaactat ttccacgttt gactaatctt
    67561 gccataattg aatcagctct ctttctctga tacaatagga actataaaga agcccattgt
    67621 ttgggtttta ttattttttg cacatccttt ttgcttgccg gcgcaaggat gtgctatttg
    67681 tcgttttaat ggtataattg gatgtgtatt agatcatcca atcagaggtg tttattatga
    67741 ttaaagtgat ttttaaaaga ataactgaac ttccagagtt tagattattt gttgattatt
    67801 ggtatgtctg gttttttatt gttgcattgc tgataggtct agatgtcata ttagaaagaa
    67861 aacttaagta agagttgagt taataaattg aattcctaaa tgagatattt tgtagcccat
    67921 attttgtttg ccgacatcac ctaatactat ggagctaatc gttttttcta acagaccaaa
    67981 atcagataat ctatttaagc taccatctat tagctcttgt gaatagccag acatgctgat
    68041 atttccagtg aaattttcgt ttccacgact cattaagcat gctaacaatt gaatctcaat
    68101 cgatgttaat tgattcagta caagaagata atactcctcg ttatcccatg aagcgttgtt
    68161 aattcgaagt aaacaattct tataaagatt tttatacata tctattttat tttgtgcctt
    68221 tgatttttct acctcgtcat ttatcccttc gaatattcca atgagttggt ccttgttctc
    68281 taagttgaaa acatcttctg gaatttgatc gcgaatgttt tctaatgatt cgtttaaatt
    68341 tttgtagaat ttctcgatgc gtttgaaacg cttctcgttt ttagacccaa aataaagtga
    68401 ctgcacaacg ccaccaatat aaggaattgc tccaataccg ccttcaacta ttaattcaag
    68461 atattctttt ttggttagtt tttcattgct attatccata gttacctcct attttgtcat
    68521 ttcctttaca agcattgtga ctcctgatgg cactcttttg tataggtttt attattgtgc
    68581 acaatctaac tcttagtggg gcgtggttca tttatttttt acttacttta ctattaatca
    68641 tatcccattg agcataggat ttttctatgt tcgatcctct tgaactaatt tctcctgtaa
    68701 aagttacttt atcgcccttt gtaaaggtat cagcattgtt taagtccttc gcaaaaatta
    68761 catatgaaac tttaggtgtt gcagaaatat cattccaact tttatcatcg tactctttgt
    68821 ctgctataat ggctattcta gtagtcattg attcgataac agttccttca aaagtatatg
    68881 ttttaccact aatatattta gtaaaagctg tattttgctc agtagctgga gtaatagaat
    68941 aataactatc taagaaagca ccccagtcat gattatttgt ctctaaaaat tcttcaaatt
    69001 gagttaggct tttattgctg ctgttagatg tagtagtttg agtatcagat gatgagcttt
    69061 cctgtttgtt attgtcattt gcctcgtttg atgaggaaag cgagaaacct acggtagtaa
    69121 aaataatccc gataataagg agaatcatag gtgttttctt atttgttttt ttgataaatc
    69181 ttacaattaa catgattaaa cctataataa ataaaacaat accagtaaaa ccaaatatac
    69241 cgtccattaa tagatcctct ttctcttttg atattgaata ttataaacca agcaattctt
    69301 ttttcttaaa atcgaattct t
//
LOCUS       DAYYBH010000007        69157 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000007 DAYYBH010000000
VERSION     DAYYBH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.007"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..536
                     /locus_tag="ACHAUG_000522"
     CDS             <1..536
                     /locus_tag="ACHAUG_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8479640.1"
                     /translation="TTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPT
                     PEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVT
                     GRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGES
                     VKVGSVQWFAPDFAVSM"
     gene            complement(604..2403)
                     /locus_tag="ACHAUG_000523"
     CDS             complement(604..2403)
                     /locus_tag="ACHAUG_000523"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8479641.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            2526..3032
                     /locus_tag="ACHAUG_000524"
     CDS             2526..3032
                     /locus_tag="ACHAUG_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8479642.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            3111..3833
                     /locus_tag="ACHAUG_000525"
     CDS             3111..3833
                     /locus_tag="ACHAUG_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8479643.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3911..5311)
                     /locus_tag="ACHAUG_000526"
     CDS             complement(3911..5311)
                     /locus_tag="ACHAUG_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8479644.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(5481..6455)
                     /locus_tag="ACHAUG_000527"
     CDS             complement(5481..6455)
                     /locus_tag="ACHAUG_000527"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8479645.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            6808..7368
                     /gene="pth"
                     /locus_tag="ACHAUG_000528"
     CDS             6808..7368
                     /gene="pth"
                     /locus_tag="ACHAUG_000528"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8479646.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            7385..10906
                     /gene="mfd"
                     /locus_tag="ACHAUG_000529"
     CDS             7385..10906
                     /gene="mfd"
                     /locus_tag="ACHAUG_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8479647.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            10921..12516
                     /locus_tag="ACHAUG_000530"
     CDS             10921..12516
                     /locus_tag="ACHAUG_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8479648.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            12527..12796
                     /locus_tag="ACHAUG_000531"
     CDS             12527..12796
                     /locus_tag="ACHAUG_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8479649.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            12863..13288
                     /locus_tag="ACHAUG_000532"
     CDS             12863..13288
                     /locus_tag="ACHAUG_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8479650.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            13334..13804
                     /locus_tag="ACHAUG_000533"
     CDS             13334..13804
                     /locus_tag="ACHAUG_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8479651.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            13924..15369
                     /gene="tilS"
                     /locus_tag="ACHAUG_000534"
     CDS             13924..15369
                     /gene="tilS"
                     /locus_tag="ACHAUG_000534"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8479652.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            15369..15914
                     /gene="hpt"
                     /locus_tag="ACHAUG_000535"
     CDS             15369..15914
                     /gene="hpt"
                     /locus_tag="ACHAUG_000535"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8479653.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            16004..18115
                     /gene="ftsH"
                     /locus_tag="ACHAUG_000536"
     CDS             16004..18115
                     /gene="ftsH"
                     /locus_tag="ACHAUG_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8479654.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            18332..19219
                     /gene="hslO"
                     /locus_tag="ACHAUG_000537"
     CDS             18332..19219
                     /gene="hslO"
                     /locus_tag="ACHAUG_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8479655.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            19220..20224
                     /gene="dusB"
                     /locus_tag="ACHAUG_000538"
     CDS             19220..20224
                     /gene="dusB"
                     /locus_tag="ACHAUG_000538"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8479656.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            20335..21831
                     /gene="lysS"
                     /locus_tag="ACHAUG_000539"
     CDS             20335..21831
                     /gene="lysS"
                     /locus_tag="ACHAUG_000539"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8479657.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(21946..23220)
                     /locus_tag="ACHAUG_000540"
     CDS             complement(21946..23220)
                     /locus_tag="ACHAUG_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM8479658.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(23405..24415)
                     /locus_tag="ACHAUG_000541"
     CDS             complement(23405..24415)
                     /locus_tag="ACHAUG_000541"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8479659.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(24595..24939)
                     /locus_tag="ACHAUG_000542"
     CDS             complement(24595..24939)
                     /locus_tag="ACHAUG_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8479660.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(24932..25366)
                     /locus_tag="ACHAUG_000543"
     CDS             complement(24932..25366)
                     /locus_tag="ACHAUG_000543"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8479661.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            25504..26241
                     /locus_tag="ACHAUG_000544"
     CDS             25504..26241
                     /locus_tag="ACHAUG_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8479662.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            26234..27445
                     /locus_tag="ACHAUG_000545"
     CDS             26234..27445
                     /locus_tag="ACHAUG_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8479663.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            27649..28431
                     /locus_tag="ACHAUG_000546"
     CDS             27649..28431
                     /locus_tag="ACHAUG_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8479664.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            28501..29163
                     /gene="trmB"
                     /locus_tag="ACHAUG_000547"
     CDS             28501..29163
                     /gene="trmB"
                     /locus_tag="ACHAUG_000547"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8479665.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(29261..31015)
                     /gene="ade"
                     /locus_tag="ACHAUG_000548"
     CDS             complement(29261..31015)
                     /gene="ade"
                     /locus_tag="ACHAUG_000548"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8479666.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            31147..32076
                     /gene="comGA"
                     /locus_tag="ACHAUG_000549"
     CDS             31147..32076
                     /gene="comGA"
                     /locus_tag="ACHAUG_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8479667.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            32033..33082
                     /gene="comGB"
                     /locus_tag="ACHAUG_000550"
     CDS             32033..33082
                     /gene="comGB"
                     /locus_tag="ACHAUG_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8479668.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            33079..33357
                     /gene="comGC"
                     /locus_tag="ACHAUG_000551"
     CDS             33079..33357
                     /gene="comGC"
                     /locus_tag="ACHAUG_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8479669.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            33332..33802
                     /gene="comGD"
                     /locus_tag="ACHAUG_000552"
     CDS             33332..33802
                     /gene="comGD"
                     /locus_tag="ACHAUG_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8479670.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            33774..34121
                     /locus_tag="ACHAUG_000553"
     CDS             33774..34121
                     /locus_tag="ACHAUG_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479671.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            34084..34509
                     /gene="comGF"
                     /locus_tag="ACHAUG_000554"
     CDS             34084..34509
                     /gene="comGF"
                     /locus_tag="ACHAUG_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8479672.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            34481..34840
                     /gene="comGG"
                     /locus_tag="ACHAUG_000555"
     CDS             34481..34840
                     /gene="comGG"
                     /locus_tag="ACHAUG_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8479673.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            35012..36019
                     /locus_tag="ACHAUG_000556"
     CDS             35012..36019
                     /locus_tag="ACHAUG_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8479674.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            36044..37228
                     /locus_tag="ACHAUG_000557"
     CDS             36044..37228
                     /locus_tag="ACHAUG_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8479675.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(37665..39248)
                     /gene="abc-f"
                     /locus_tag="ACHAUG_000558"
     CDS             complement(37665..39248)
                     /gene="abc-f"
                     /locus_tag="ACHAUG_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8479676.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(39353..39481)
                     /locus_tag="ACHAUG_000559"
     CDS             complement(39353..39481)
                     /locus_tag="ACHAUG_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479677.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(39826..39969)
                     /locus_tag="ACHAUG_000560"
     CDS             complement(39826..39969)
                     /locus_tag="ACHAUG_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8479678.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(40192..40671)
                     /locus_tag="ACHAUG_000561"
     CDS             complement(40192..40671)
                     /locus_tag="ACHAUG_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8479679.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(40744..41028)
                     /locus_tag="ACHAUG_000562"
     CDS             complement(40744..41028)
                     /locus_tag="ACHAUG_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479680.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(41106..41300)
                     /gene="ssrS"
                     /locus_tag="ACHAUG_000563"
     ncRNA           complement(41106..41300)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHAUG_000563"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(41372..42658)
                     /locus_tag="ACHAUG_000564"
     CDS             complement(41372..42658)
                     /locus_tag="ACHAUG_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8479681.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            42796..43467
                     /locus_tag="ACHAUG_000565"
     CDS             42796..43467
                     /locus_tag="ACHAUG_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8479682.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            43433..43921
                     /locus_tag="ACHAUG_000566"
     CDS             43433..43921
                     /locus_tag="ACHAUG_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8479683.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            43934..44881
                     /gene="prmA"
                     /locus_tag="ACHAUG_000567"
     CDS             43934..44881
                     /gene="prmA"
                     /locus_tag="ACHAUG_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8479684.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            44882..45646
                     /locus_tag="ACHAUG_000568"
     CDS             44882..45646
                     /locus_tag="ACHAUG_000568"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8479685.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            45879..48092
                     /locus_tag="ACHAUG_000569"
     CDS             45879..48092
                     /locus_tag="ACHAUG_000569"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8479686.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            48103..48549
                     /gene="dtd"
                     /locus_tag="ACHAUG_000570"
     CDS             48103..48549
                     /gene="dtd"
                     /locus_tag="ACHAUG_000570"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8479687.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            48720..49979
                     /locus_tag="ACHAUG_000571"
     CDS             48720..49979
                     /locus_tag="ACHAUG_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8479688.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(50068..51015)
                     /locus_tag="ACHAUG_000572"
     CDS             complement(50068..51015)
                     /locus_tag="ACHAUG_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8479689.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            51409..52701
                     /gene="hisS"
                     /locus_tag="ACHAUG_000573"
     CDS             51409..52701
                     /gene="hisS"
                     /locus_tag="ACHAUG_000573"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8479690.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            52716..54485
                     /gene="aspS"
                     /locus_tag="ACHAUG_000574"
     CDS             52716..54485
                     /gene="aspS"
                     /locus_tag="ACHAUG_000574"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8479691.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            54594..55469
                     /locus_tag="ACHAUG_000575"
     CDS             54594..55469
                     /locus_tag="ACHAUG_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8479692.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            55735..57618
                     /locus_tag="ACHAUG_000576"
     CDS             55735..57618
                     /locus_tag="ACHAUG_000576"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8479693.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(57693..57977)
                     /locus_tag="ACHAUG_000577"
     CDS             complement(57693..57977)
                     /locus_tag="ACHAUG_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8479694.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(57967..58701)
                     /locus_tag="ACHAUG_000578"
     CDS             complement(57967..58701)
                     /locus_tag="ACHAUG_000578"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8479695.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            58830..59453
                     /locus_tag="ACHAUG_000579"
     CDS             58830..59453
                     /locus_tag="ACHAUG_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8479696.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            59645..60766
                     /locus_tag="ACHAUG_000580"
     CDS             59645..60766
                     /locus_tag="ACHAUG_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8479697.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            60773..63901
                     /locus_tag="ACHAUG_000581"
     CDS             60773..63901
                     /locus_tag="ACHAUG_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8479698.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            63920..67114
                     /locus_tag="ACHAUG_000582"
     CDS             63920..67114
                     /locus_tag="ACHAUG_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8479699.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(67260..67967)
                     /locus_tag="ACHAUG_000583"
     CDS             complement(67260..67967)
                     /locus_tag="ACHAUG_000583"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8479700.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            68732..69130
                     /gene="spxA"
                     /locus_tag="ACHAUG_000584"
     CDS             68732..69130
                     /gene="spxA"
                     /locus_tag="ACHAUG_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8479701.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        22212 a        12348 c        14671 g        19926 t
ORIGIN      
        1 caacaacagt gcctgaatct tcaacaacag aagaatcaac aacacctgcg cctacaacac
       61 catcaacaga tcaaagtgtt gatacaggaa acggcacagg aagtagtact ccggctccaa
      121 cgccaacacc aacacctgaa caaccaaaac ctgtaacacc tgctccagct ccttctggaa
      181 gtgtgaatgg tgcagcaatc gtagcagaag cttacaaata tattggtact ccatatgttt
      241 ggggcggtaa agatccaagt ggctttgact gctcagggtt cacacgctat gtttacatgc
      301 aagtaactgg tcgtgacatc ggtggttgga cagttcctca agaatcagct ggaacaaaaa
      361 tttcagtttc tcaagcaaaa gctggagact tgttattctg ggggtcacaa ggcggaactt
      421 accacgtagc gattgcttta ggcggaggac aatatatcca tgctcctcaa ccaggcgaaa
      481 gcgtaaaagt tggttcagtt caatggtttg cccctgactt tgctgtcagc atgtaagaat
      541 gattaatcaa aaagagttag cccattctgg actaactctt tttttattgt gataaaatag
      601 aaatcagata aattcttctg tcaattccat gaacgtttgg atatcttcag tcaccatgtc
      661 gatacctgct tgccaaaaat ctggttgggt aagatcgaca ttcaaatgtt tcttcgcaag
      721 atcttccgtc gtcattgagg cagtatcgcg tagtaaatgg atatattctt cttcaaaatt
      781 tgctcccttt tggttggcgt aagcatagat gcccaaacta aagaggtagc caaatgtgta
      841 tgggaagtta taaaacggta cgtcatcaat aaagaaatgc aattttgcag cccagaaata
      901 tggatggtaa gtagctaaag ctccttgata gccttctttt tgcgcctcaa ccatcatctc
      961 tgtgatctta tcttctgaga ctaatccttc ttgccgtgct tcatagaatc gattttcaaa
     1021 aatgaatcgg gaatggatat tcatgaacat agctaaagca ttttgcagtt ttgtatctag
     1081 caaattgatt ttttcttctt ttgtttttgc gacttttaat gtagcatcgg ctacgatcag
     1141 ttctgcgaat gtacttgctg tttctgcgac attcatggca tattcgcgat ttaaagaagg
     1201 taaatcccac atcgtgctgc tatggaaagc atgtcctagt tcatgagcaa gtgtcgcaac
     1261 ctcattaaca gaattactgt aggtcataaa gatccttgat tcttttgttt ctggtaactc
     1321 agtacaataa ccaccaggac gtttcccagg acgatcttct gcttcgatcc agctcttttc
     1381 aaaagcagat tgggcaaatt tagccatttt tggactgaat ttgttaaagt tttcgataat
     1441 aaatgctgct gcttcgtcaa aactaaaggt tttttctttc aaatcgccta aaataatagg
     1501 tgcatcttga tcttgccatt ccattttttc tttaccgaaa agattagctt ttctagtcaa
     1561 gtaattaaca aggggttgtt tgtttttctg gattgtatcc cacatgacag acaatgtttc
     1621 tttcttcaac cgattgtact ctaatggttt ttgtagaaaa tctgtgacac catgaagttc
     1681 atagtcagaa agtctaaaac catctaaatg attcaaagta tccgtgaaga ggggtgcctt
     1741 ttctttccag gcttcttccc atgctgcgaa aagtgtttct ctgacttttg gatctggatc
     1801 gcccatcatc ttattgaagg cttgtccagc agataattcg actacttgtc catcttgttc
     1861 gaagggaatc gaaatgctgg ctacaatcgt atcataatga ctgctccaag catttagacc
     1921 atctaaagaa agcgtattta taatgttttc ttcagcttct gataataatt gtgagccatc
     1981 acgacgaatc tcgtttaaac gaaaagcaat tgtttcaaac gaagattgag aaagcatctg
     2041 attccaatca ttgtcagata tttctgcaaa tttctttgac aatacagttt cggctgattg
     2101 cagacgaggc agcattgcgt aaagcttgcc tgataagacc tttgcatctg aatcattgac
     2161 attggcagaa agcaatgcag taatatagct gctgcattgg gtaaaaccat tcgtaatcgt
     2221 ttcttgcaat tgaagaatag cgtttaactg ctctgcattg tcagaactag gtgaccattt
     2281 tgttacgcgg tgataatact cgttcatttg gtcttccaat tgcttcatcc gttggttcaa
     2341 agcatcagaa tgactgcctc cggaaaaaat agaatctaga ttccaattta atgaataact
     2401 catgaaaaaa ctcccttcat attgtaatac aaaattaagt atagcacttt tttatggaag
     2461 aagtctctat gaaaatgatt gttgtttctt caaaaaaagt ctaagataaa atacagaggt
     2521 gacttatgaa ttcttcaaaa aaacaaatga aaaacggaaa tatttattta gtaatcgcac
     2581 tgatagtcgt tataatctta ttttacagtt cttctcaaac ttacggacag caatcacaag
     2641 tttcgcgatt agaaagctgg ctgcctaatc agccatttaa agacacatta gcgggtatac
     2701 gttttaatta tggtggaagt atggtgagta tcaaccatct gggctacgcc aaatttttag
     2761 agttcttttt aaggaagggc gcacatttcg gtacctattt tattttggga ggcagcttgt
     2821 ttttaggtgt tttccccaag ctaaaaattt ggtggctgac agcgatcctt gcttggcttt
     2881 cagcaacagg gtacgcaggt ttggacgaat tccatcagat gatgaccggt ggacgaacac
     2941 ctatgttcca agatgtcatg ttggattcga tcggtgctct gactgctgtg atattatgtg
     3001 tgctttttgc ggcattcaag aagcgtttat agattggctg tggctttgtt tttttgtgaa
     3061 atagtactag aatagaagtg tattctcaaa agaacaaggg aggaaaaatc atgtcaggac
     3121 atagtaaatg gcataatatc caaggaagaa aaaatgccca agatgcaaaa cgagggaaag
     3181 tctttcagaa actttctcgt gaaatttata tggcagctaa agcaggcggt cctgatccat
     3241 ctatgaatcc agctttgcgg cttgttgtgg acaaagcaaa agctgcaaat atgcctaacg
     3301 acaatgtgga acgggcgatt aaaaaggcta cgagtgccgt agatgaaacg aattacgatg
     3361 aaatcacgta tgaaggatat ggtccgggtg gagtaggaat cttagtccat gcattgacag
     3421 ataaccgtaa tcggacagcg acaaatgtcc gagtagcttt tactcgaaac ggtggatcac
     3481 tgggagaaac gggttcagtc agttacttgt ttgaccgcaa aggatatatc gctattgaac
     3541 gcgatgggtt gactgttgat gaagagacga tgtttgagga tgtattagag gcgggggcag
     3601 aagatctgca aacttctcct gaagtattcg agatttatac tgcaccagaa gattttgctg
     3661 ctgtacgaga tgagttggaa aaagagttca ctttggcgca agcagaattg acgatgattc
     3721 cacaaacgac gattgattta aatgaggaac aaaaagagca attggaacgt ctagtagata
     3781 agctagaaga cgatgatgat gtttcagaag tctttactgc tgctgaaatg taagtagcac
     3841 attgttagat aataaaaaga aagcacctgt accaatcgta cgggtgcttt ctttttatat
     3901 attatttttg ttagaagacc aatacaggcg ttcctttttc gaccatcccg aataattcct
     3961 tcattacact tggtggggtg ttgatgcagc cgtgtgaacc gcgggttttc cataagtctc
     4021 caccgtattc aggttgccag tctgaatcgt ggataccaac acctgtccag tcaatcggca
     4081 tccagtagtt taccggactc tcgtaaggtg ttccatcgtc atttgttcct tttaaagttg
     4141 catcttcttc tttattccaa acatagaaga ctccagcagg tgtaggtgta gttggctttc
     4201 cggaaacgat atctgtttca agggccactt ttccgtcttt gtaataccac atatgctggt
     4261 tctccagatc aacttcgata tacgtatctt cgatcaatgg atgatcagcg gttgtgccac
     4321 cttgcacaat gggtgaacga gtaaaatctt ggcctgccag aatcgctttt ttcaatgctt
     4381 ctgtctcact gtctgtttgg atcgtccaac tgtaagtgcc aactgggacc gtaacttctc
     4441 ctctttttgt acttttaaat tttgtgtcat tcgtacttgt gttatacttt gtgcctaaat
     4501 ctgttacata ttgacgtact tgttctgtat ctaaatcgac ttttccatca ttgtaggtca
     4561 gccaactcat aatatcactt gatgggatct ggaaagtctc accgtttata ctgtaagtag
     4621 cttttacatt tgcgatagca ttcatggatg cgagttgttc tttcagtgag ctgtcttcag
     4681 atgttacttt tggtttttct ttgaattcag tcaattcaat tgtatcttta ccagagttta
     4741 ccgcactttt taattgtttg accgcagcat cgacgtcgat cgtatttcca ttgacttcag
     4801 gcttgataac gaaagagtca cctgcttttt cgatcgttgc atcagtagta ggtgtccggt
     4861 ctttgttcaa atcagtcagc ttagttgtca atgtctgaac ggtagtgtct agtttttggt
     4921 ggttttgact gacaggatct atttcttgtt tttctgccgc tgagacataa gtcattcccc
     4981 aagtccatcc gttttgttca tttaggatcg gagaaagctc cgaagtgaaa tcatctttgt
     5041 agccgagctc ggattttggt acttcctgcc agacttttcc atcttcttta atagccagtt
     5101 ttttatcaga ataagcttct tttaattttt catttgcttt ttcgactgtt agattgctga
     5161 catttattcc atttatttct gtattcggca aaaaatgctt tgtgtagtat gtactgcgat
     5221 aggtataaaa gctgatcagc actaagagaa caccaagaac tgaaagcaaa gcaacatgtt
     5281 tcgatttttt tcgatgcgaa gatcttgtca tatcgacaac tccttaagta aaagtttagg
     5341 gtggaacggt ctcatgatag cataatttag aggaaaagac gaaggttatc tctactttat
     5401 gaaaaaattt agaaaccata agggtgataa aataaaaaaa cggaaaacac cgaatagatg
     5461 ttttccgtca tgttcaaaga ttattcagcg tctagtttag caaaagcttc atctagaatt
     5521 tcttttaatt gcttagcaga agcagccata cggtcttgct cgctgtcact taaaggaatt
     5581 tcgattactt tttggatacc ttgacggttg ataacagcag gtgcaccaat gtagatgtcg
     5641 ttcaatccat attcgccatt catgaatact gataaaggaa gaacagcatt ttcatcatct
     5701 aagatggcac gagtgatacg tgctaatgct actgcgatac catagaaagt agcgcctttt
     5761 ttctcgataa ttgtgtaagc agcgtcacgt acgccaaaga ataggttaac catagcttct
     5821 tcatcgattt ctgggttgtt ttttacccat tcatagattt gcaagccggc aacatttgca
     5881 tgtgaccaaa ctgggaattc tgaatctccg tgttcaccta agatgtaagc atggacgtta
     5941 cgtgcatcaa cgtcaactaa ttccgcaagt gcttgacgga aacgagctga gtcaagagag
     6001 gtacctgaac cgattacgcg ttctttaggg aaacctgaga atttccaagt tgagtaagtc
     6061 aaaatatcta ctgggttagc ggcaactaaa aagataccgt taaatcctga ttcaacgatt
     6121 tgtgtgacaa catcgcggtt gatttttagg tttttgttta ctaagtctaa acgagtttca
     6181 cctggttttt gtggtgcgcc agctgtaata acaactaagt cagcatcatg tgcgtctgcg
     6241 taagtagcag aatagatttt tttaggtgaa gtaaatgcca aagcgtgaga caagtcaatt
     6301 gcgtctcctt cagttttagc agtattgata tccacaatac ctacttcttg agcgatattt
     6361 tgagtaacta gagcaaaggc atagctagaa cctacggcgc cgtctccaac aagaataact
     6421 ttttgatgat cttttttttc tgcagctgca gtcatttagt atcaatcctt ccattgattt
     6481 gttttttccc atgcttatgc taacatttct caagccccat gtcacgctat ttggctataa
     6541 aatataacag ataacaaggt tgtattatat gtaaaagctt acatgtgcgt tatttcacta
     6601 tttttatata tcaattattt cattaaactt tgaagaaatt tttaatttct ttatcattta
     6661 ttaacgattc tcttttagcc gaaacgcctt gttttgtgtt atactatgca ttgacaagtg
     6721 taaaaaagaa aaaagctcga tggactggac cgctaattag cagtccagcc ttttgtgctg
     6781 ctttttagaa ttaggtggaa aacaaaaatg aaaatgatcg tcgggttagg aaacccaggt
     6841 accaaatacc aatatacaaa acacaatatc ggctttatgg tcgtggataa aattgcacgc
     6901 gagcatcaag ctactttcaa aaaaaatccg ttcgaagcag aagttgccga attttttcat
     6961 aatggagaaa agatcctttt agtcaaacca caaacattca tgaatgaatc tggtcgtgca
     7021 gtcggcccac taatgactta ttttgggatt tatccagagg aattagtcgt aatctacgat
     7081 gatttggatc tggcagtggg aaaaatccgg ctccgtcaaa agggaagtgc cggcgggcat
     7141 aacggtatca aaagtattat ttcccatttg aatacgaatg tctttgatcg aatcaaggtg
     7201 ggaatcggca ggccagaagg gaaaaaaaca gtagtccagc atgtgcttag tcctttcagc
     7261 aaagaaaatc agcctttgat agaagaaagc atgtgtcaat ctgttaaagc agtcgaatat
     7321 ctcatagaag ggcattcgtt cgtggatgcg atgaatcgat tcaattgagg aaggaggaaa
     7381 aggaatggat attattcaat tagtcggaaa agatccgcaa gtaaaagaat gggtcagcaa
     7441 tttagaagaa aaaatgccta gacaactaat cacaggatta gcgggctcag cgaaaacact
     7501 tctatttgct cgagcgttta agaaaacaaa taaaaacatg attattctga tgccaaattt
     7561 gtattatgcg aatcagttag cagaagatct gcaacatgtg attccgcaag atcagatcca
     7621 tttatttcca gtggatgaag tgctttctgc tgaaatggcc ttttcttctc cagaagcgcg
     7681 aggcgagaga gttgcaacgc ttaacgcact tcaagaaaaa aaagcaggta tctatttgtt
     7741 gccagtcgca tctttgcaca aacgactgcc gaataagaaa acttggctac aggcgcaatt
     7801 gaattggcat attggtgatg agatagacgt ggaaaatctt cctcgccaat tgactttgat
     7861 gggctatgta cgagaagaga tggtcgcaaa accgggagaa ttcagtattc gaggaagcat
     7921 tatcgatatt tacccgttaa cgacaaccta tcctgttcgt gtagaactat ttgatgtaga
     7981 gatagattct atgcggtatt ttgaagcaga aacacaacgc tcaatcgaaa aaacggaaga
     8041 aatctgggtt tccccaacga gtgaacaagt ctacggacca gaggatctag aacatggaat
     8101 agagcaaatg caaaaaatgc tagaaaaacg gcttgctgcg accacagaag aagcagaccg
     8161 cgagtttttg caagattact tcggtcagct gacttcaagc tggcaagcag gtgtcccgac
     8221 agaagaagca aaattctact cagatcttct ttaccaagaa tctgtgacga tcttggatta
     8281 tttcccttca aatagtttac tagtcgttga tgattatcag cgaatcatgg aaacaaatcg
     8341 ggaaatcgaa agggaagcgg cagaatggca gactcaaaaa atcagcgaat tgcgggtgtt
     8401 ttctgagcag acattttcag cagatataca aaaaatcgtg cagaaggaaa agttcaccac
     8461 aacctttttc tcactcttcc aaaaagggat gggaaatctt cgtttccaag caatccataa
     8521 ttttcaatat cggacgatgc aacagttctt cggacaaatg ccattgctga aaacagaaat
     8581 ggaccgttgg cgcaagcaaa aacagactgt tgtgatcttt attccgacaa aggacagaat
     8641 acgcaaagca gaacaaatgc ttcgagatga agacatatta gtagtagaaa cagaaaaaaa
     8701 tgcattgatc agaggacagg tacaattagt tgaaggagct ttgcaggctg gttttgaact
     8761 tcctcaagaa aaaatagtag ctatcacaga aaaagaaatt ttccaaaaaa cgacgaaaaa
     8821 acaaacgaga cgtcaaacag taacaaacgc agagcgattg aaaagctata atgagttaaa
     8881 agcaggagat tacgttgtcc atgccaatca tggtatcggg aaatacattg ggatggaaac
     8941 gctagaagta gatggcgttc accaagacta tatgactatt ttgtatcaaa acgacgacaa
     9001 actgtttatt cctgttactc aactgaatct gatccaaaaa tatgtcgcct ctgaagccaa
     9061 agcgcctcgc atcaataagc taggcggaag tgaatggacg aaaaccaagc gaaaagtctc
     9121 ttcaaaaatc gaggatatcg ctgacgatct aatcaaatta tatgccgcaa gagagtcaga
     9181 aaaaggatat gcttttggac ctgatgacgc ctaccaaaaa gaattcgaaa atgccttccc
     9241 ctactctgaa actgatgacc agttgagaag tgcagcggag atcaaacggg atatggaaaa
     9301 agaaaaacca atggatcgac tactcgtagg tgatgttggt tatgggaaaa cagaagtagc
     9361 gcttcgagcg gcctttaaag cgataaagga aagcaaacaa gtagcctttt tagttccaac
     9421 aacgatcttg gcacagcagc actatgaaac gatgctggaa agatttgaag gtttccccgt
     9481 aaatatcggg ctattaagtc gtttcagaac aagaaagcaa caaaaagaaa ccatcgaaca
     9541 actgcgtaca ggtcaagttg atattgttgt aggaacccac cgaattttgt caaaagatat
     9601 tgaatttagc gatttgggtc tactgatcat tgacgaagaa caacgttttg gtgtaaaaca
     9661 taaggaacga ttgaaacagt tacgtgcaca agtggatgtt ttgactctga ctgcaacacc
     9721 gattccgaga actttgcata tgtcgatgtt aggcgtgcgg gatctttcag taatcgaaac
     9781 accgcctgcg aatcgttatc cgatccaaac gtatgttatg gaaaaaaatc ctggggctat
     9841 tcgcgaagca atccatcgag aaatgggacg aggtggacaa gtattctatt tatataatcg
     9901 ggtagaaacg atcgaacaaa aagtagaaga aatccaagaa ctcgttccgg aagcacggat
     9961 cggttatgct catgggcaaa tgacagaggc gcaattagaa aatacgcttt ttgaatttat
    10021 tgaaggtcaa tatgatgttc ttgtcactac aaccatcatt gaaacaggag tagatatccc
    10081 gaacgcaaat acgttgttcg ttgagaacgc tgattatatg ggattatcca cgttatacca
    10141 attgcgaggg cgggtaggaa gaagcagccg tgtcgcatat gcttacttta tgtatgagca
    10201 gcagaagatc cttaacgaag tcagtgaaaa acgtcttcag gcaatcaaag attttacaga
    10261 actgggatct ggattcaaga ttgcgatgcg ggatctttcc attcgcggtg caggtaatct
    10321 cttaggtgct cagcagcatg gatttattga tgcagtcggt ttcgatatgt attctcaaat
    10381 gctaagcgaa gctgtttctc gtaaacaagg aaagaatagt caagtggaga agaccactgt
    10441 tgagatcgac ctcggtgtcg atgcgtatct acctgaaaca tacgtggcag atcaacggca
    10501 gaaaatcgag atctataaac gaattcgtga acttgattcg caagaaatgc tagatgaact
    10561 agaagatgat ctgctcgacc gttttggaga atatccagaa gaagtagcac atctcttgac
    10621 aattgggcag atcaaaatgg atggcgatcg agcgctccta gaaacgataa ggaaacagga
    10681 ggcgcatata gtctttactc taagtaaagt tggaacaaaa cgctatagtg tggaacagtt
    10741 attcgaagca ctgacagcga caaaactaaa agccagtcta ggcgttgaaa aagaacagat
    10801 ggtgatacgc ttgacgatcc ccaaccaaat ggaagaagca gtatggcttc aggaaatcca
    10861 aaagtttgtc aaagcgttgc gtgagcaaaa atatctgtca gcgtaggaag gagcaatgat
    10921 ttggatcata aagaaatgcg gcgcgtcatg caaggagcat ttgtattgac gattgcttct
    10981 ttcatagcaa aagtattaag tgccctttac cgaattcccc tccaaaactt agtcggcgac
    11041 gaaggttttt atgtttatca gcaagtttat ccaatttatg ggatagcaat gactttagct
    11101 ttatccggtc ttccgcagtt catatcaaag tacgttgcag aaagaaagaa tcctttcgag
    11161 cgaaagcaag ctttgcaaaa tctttatccc ttagtatttt ggacaggtgt ctttttgtgg
    11221 ttgtttgtct ttgcatttag cagctggatt gcttggatga tgggagacat acaattgaaa
    11281 ccgctgatcc aagtagtttc ctttaccttt ttgctgatgc ctggtctatc tttttatcga
    11341 gggaatttcc aaggaagatt cttgatggaa ccaactgctg tttctcaagt agttgagcag
    11401 ttcgttcgtg tttttgtgat tgtctgtagt gcgtttgctt ttagaaagct tggatggacg
    11461 gtgtaccaaa cagggacggc agcaatgagt ggagctgttt ttggcggttt atgcgcatat
    11521 gggatccttc actactatga acaaaagatt catggaggag cgttaagctt ccgacggttt
    11581 ccattgttca aacagccgtc aaagcccttg atccggcgtt ttctggtaga aggcggactg
    11641 gtttcgattt atagtggatt gcttatcttt ttccagctga tcgactcttt ctttgtaaaa
    11701 aatgctttgg aagtttacgg actgtcagaa tacggtgcga agatagcaaa aggggtctat
    11761 gatcgaggtc agcctttagt acaattaggt ttagtgattg ccactgcatt aagtgcgaca
    11821 ttcctgcctg cgttgactcg tcatttgacc aaccggatct atcgtcagtt tttacagaca
    11881 gccaaaatct atcttagatt gacgaccgct ttagcattag ctgcgtcttt gggattagcc
    11941 ttgctgctcc cttatatcaa ttatgcgttg tttaaagatt atgcgggaaa tgcagcgctt
    12001 gtattatttg ttttttccat tgctttcact gcagtcatcc aagcttatca aagtattgct
    12061 caaagcaaga actcgtttcg cccttcttta aaaggcgcag gatggggact attggtaaaa
    12121 ggattgacta cttcttttct gaccggttta ttaggaacag caggtgcaag cctgtctacc
    12181 cttttaggat taggtgtgac gctttggtat tttgttcgaa ttgaaacaaa agagctaaat
    12241 gctttttgga aagaacggca ttttggacgg aagttgatgt tcagccttct ttgtatgaca
    12301 gtagtactgc tgctgtatta tggtgtattg aatatagtat ttggtcctgt aactcatcgt
    12361 tccactgctt tttttgtcag cgtgatagga gttgggatcg gggcttctgt gttcatttat
    12421 gcagtgattc gtcttcgtct gtttacagta cgagaatggc taatgttgcc ttttggtaag
    12481 aaaatcctgc gattaaataa aaaaattaaa aagtgaggaa taagatatgc gattagataa
    12541 atttttaaaa atttcccgaa tcatcaaaag acgatcagta gcaaaagaag tagctgataa
    12601 aggaagaatt caaatcaacg gtaaattggc gaaatcttcc agtgatgtca aagtaggcga
    12661 tattttgaaa gtccagtttg gcaacaaaac gttggaagtg aaaattacgg aattacatga
    12721 atcaaccaaa aaagaagatg cacaaaaaat gtacgaaatt atttctgaaa atcgcacaga
    12781 aacactagag aaatagatta gacaagcttt cacgatattg gtataatgaa aaagacaatc
    12841 aaaaagattg gagcgaatcc aagtgacgaa aaaaagcaat aaaatcgctg cgctagatac
    12901 ggaatatgca aagaaaaagt atgctgaatt tcagcaacaa caacgtcaac tgatttttcg
    12961 aagacgcaga ctggctgcaa tttttgttgg tgctttggtc attttcgcga taataggtgt
    13021 gcagattttc catgacatgc aaaggatgca tcaattgaac gaattgagag tcgaagcaaa
    13081 tactgaaatg aaagaagtca acgcagacgt tgatcagctg caccaagatg tttcattgct
    13141 gaaagatgat gagtatgtag ctaaacttgc cagaagcaaa ttcttttact caaaagacgg
    13201 tgaaaaagtt tatcctatat tagaatcaaa tacagacagc tcgacaaatg aaacagatca
    13261 atcaactagt tctacaactg caaaataagc aggaatataa aaaagggaaa taattaggag
    13321 gaacattttt tttatgtcaa tcgaggtagg agcaaaactg caaggaaaag tatcaggaat
    13381 cacaaatttt ggtgcgttta ttgatctagg tgctggaaaa acgggtctag ttcatattag
    13441 cgaagtgtca aatggctatg taaaagacat ccatgatgtt ttaagtgtag gagatgaagt
    13501 aacagttaaa gttatttcag taaatgatga tggaaaaatc ggactatcta tccggaaagc
    13561 gcaggaacaa cctgctgaat ctggagaaga aagccgtcct aaaaaatatc aacaaaaaga
    13621 tccccgtgac aaccgaaatc gtccagcaca gaaaaaatct ttttctcgca gtcaaccttc
    13681 agctcaaaaa gaagacttcg attcattgat gagttcattt ttgaaagata gcgatgaccg
    13741 cttatcttca ttgaagcgaa acacagaagg aaagcgcggt ggccgtggcg gacgtcgcag
    13801 ctaaaaagcc gcaaataatg atagtgcgac tatttgggtc atagtatcaa cagagggcag
    13861 gttaattgtc gttttagacg ttttcccgct ctcttttttt taggaagaaa gggtgtggaa
    13921 aggatgaacg ttgaaaaagg cgggaaaaaa gcaccgctat tacagcagtc atttattcgg
    13981 cacgggaatg aacaaggatt ctggcaacaa ggatcgagaa ttttagcagc ggtatcaggt
    14041 ggtgttgatt cgatggtttt gctccattta cttttacaag tgcaacgcca aatcggtttt
    14101 tctttaggta tcgtccacat caatcaccaa ttaagagaag aatcagcaga agaagctgcc
    14161 tatcttcgct catattgtga agagaagaat ctccctttat atgtaagtat ttgggaagat
    14221 ccagccaaat ccgccataga aacagaagct cgtaactttc gttatcaaga attcactcgc
    14281 gtgatggaag aaggacaata tgacacgttg atgactgctc atcacggaga tgatcaaatc
    14341 gagacggtgt taatgaaaat cgttcgtggg ggacagttaa atactttttc tggaatcaaa
    14401 gaagtacaac catttgctac gggcagactg attcgtccgt tgctgagttt tagcaaagaa
    14461 gacctatata cttatgcagc ggaatcgcag cttgtttact ttgaggatca gacaaaccaa
    14521 ttgttagata tccagagaaa tcggcttcgt catttggtag ttcctcaatt gaaacaagaa
    14581 aatacacagg cgttgagaca tttccagcaa ttttcgcaac agatccaatg ggcagaccaa
    14641 gtcattcaaa aatatatggg acagttgatc gaaaaggaag tcgaacagtg ggaggaacaa
    14701 tttcgatttt ctgcagaaat gattgaagaa atgacagatg ctgaacgata ctattttttt
    14761 tactcctttt tcgacaaagc attctcaaaa acaggaatta ttttgaaaga acaacaaatg
    14821 gagtcgattt tagaccagtg gcaacagaaa aaaagccaat ggcaactcag tattggcaat
    14881 aattggatac tgaaaagaga gtatcatttt gttgtactct ctaaaaaaga aaaacaagca
    14941 actagccaga cagaagacca gcgcttgttg ctgattccag aacaaggaat atatttgaat
    15001 gaaacagaat ggattggtct ggtcaaacct gaaaatgcag gggacatcaa agcaacagat
    15061 tcatggtctc tattttctca tgacatttgg attcctgctg atgtgcaact gtatgtcgaa
    15121 aaaagaaaag caggagaccg cattaaatta acagagaatc tcacaaaaaa agtatctaga
    15181 tattttatcg atcagaaagt acctaattca caaaggcagc atgcttggat cattacagat
    15241 gccaaaagaa atatatatag cttgatacct tttgcctttt catatttgag tattcgtgaa
    15301 gaaactgata aaatacacta catacttctt tacaaatatc aaaaagaagc aattggaagg
    15361 agaacctgat gttggaaaag gatattgaga gaattttgat ctcacaagaa gagatacaag
    15421 ttcgttgcaa agaactaggc aaagagttaa cagaaattta ccaaaacacc aatcccctag
    15481 tagtaggtgt cttaaaagga gcggtaccat tcatggcaga tatcgttcgt tctatcgata
    15541 cgtatttgga attggatttc atggatgttt caagctacgg gaatgcaaca gtctcgtcag
    15601 gggaagtaaa aatcgtcaaa gacttagata cgaatgtaga aggtcgcgat ctgttgatcg
    15661 ttgaagatat cattgacagc ggaagaacac ttgcttattt agtggatctt ttcaaatacc
    15721 gcaaagcaaa atcagtcaaa attgttacac tacttgacaa acctgaagga agagtagtaa
    15781 atatcgaagc agattatgtt ggcttcaacg taccaaatga atttgtggtc ggttatggct
    15841 tggattatgc agaagcatat cgtaaccttc cgtacatcgg tgtattgaaa cctagtgttt
    15901 atcaatcaaa ttaatgctgt atctcaatta aattatggta caatagaatg gtcaggattg
    15961 ataaaaaact gttaaattaa attgacgctt agggagggca actatgaaca aaaagaacaa
    16021 cggcatgatg aaaaatgcct tgtattatgt cctcgtaatc ttagcgatgg taatggtcgt
    16081 ttatttcatt tttggtaata acggccagcg atcgtcagat atcgagtact caaaattcac
    16141 agaacaacta caagcaggca agatcaaaga atttgaagtg cagcctgcaa atggtgtata
    16201 taagatcact ggtgaataca aagaacagca gaccgtcgct aacagcggtg ggttagctat
    16261 cttaggttca acagatacga ctactaccca tttcacaaca atcattttgc caaatgatac
    16321 aacattgagc caagtaacag aactggcaaa agaaaacaaa gttgctacaa cagtaaaaga
    16381 agaatcaagc agcggtatat gggtttccct attgcttagc ttcttgccaa tcgtaatcat
    16441 catctttttc ttttacatga tgatgggaca acaaggcggc ggcggtggcg gtggcggccg
    16501 tgtcatgaac tttggtaaat caaaagccaa agaagcagac aaaaaagcta atcgcgtacg
    16561 tttttctgat gtagcgggtg cggaagaaga aaaacaagaa ctagttgaag ttgttgaatt
    16621 tctgaaagat cctcgccgat ttgttgaatt aggtgcccgt atcccggcag gtgtactttt
    16681 agaaggacct ccaggaacag gtaaaacctt attagcaaaa gctgttgccg gagaagctgg
    16741 agtaccgttt tactcgatct cgggttctga tttcgtagaa atgtttgtcg gtgttggagc
    16801 aagtcgtgtt cgtgatttgt ttgaaacagc gaagaaaaac gcaccagcta tcattttcat
    16861 tgatgaaatt gatgcggtag gtcgtcaacg tggtgccggt atgggcggcg gacatgatga
    16921 acgcgaacaa acattgaacc aattgcttgt agaaatggat ggcttcgacg gaaatgaagg
    16981 agtaatcgtc attgctgcaa ccaaccgttc tgatgtacta gacccagcgt tactacgtcc
    17041 aggccgtttt gaccgccaga ttttagttgg acgtccagat gtaaaaggtc gtgaagcaat
    17101 cttgcgagtc catgcacgga ataaaccgat ggcagatgac gttgacttga aagttgtggc
    17161 tcaacagaca ccaggttttg ccggagcaga tttagaaaat gtcttgaatg aagcagcgtt
    17221 agtagctgct agaagaaata aaaagaaaat cgatgcttct gatatcgatg aagcggaaga
    17281 tcgcgtgatt gcaggtccag ctaaaaaaga tcgcgtaatc agcaaaaaag aacgcgaaat
    17341 ggttgcttat cacgaagctg gtcatacgat tgtcggatta gtattgagtc gcgctcgggt
    17401 cgtccacaaa gtaacgatca tccctcgcgg acgcgccggc ggatatatga tcgctttacc
    17461 aaaagaagat cagttcttga tgacgaaaga agatatgttt gaacaaatcg taggcttact
    17521 tggtggacgt acagctgaag aaatcatctt caatgtccaa tcaactggtg cttccaatga
    17581 ctttgagcaa gcgacagctc tagcacgaag catggtaaca gaatatggaa tgagtgatcg
    17641 tttaggtcct gttcaatatg aaggaaacca tcaagtgttt gttggccgcg actatggtca
    17701 aacaaaagct tactcggaac aagtcgcttt cgagattgat caagaagtac gaaaaatctt
    17761 gatggaagct catgataaag ctcgagaaat catcgaagct catcgtgcac agcataaact
    17821 gattgctgaa aaacttttgg aattcgaaac attggatgcc aaagcaatca agtcattgtt
    17881 tgaacacggt gtgatgcctg aaggtactga gtcaaatgat tatccaagcg aaaaagaagc
    17941 gaaaactttt gaagaagcaa aacgtgcttt ggaagaaaaa gacgctgaaa aacaagcgga
    18001 agaaaaacgt gattttgaag aagcaaaaca tgaactgaat gaggaaaata aagcagaaca
    18061 tgattcagat tctcaacaat cagaagataa tcattcagac gatcaataca aataaaaaaa
    18121 tataggtcaa ttgatctgat atgactcccg aaaaccggaa caaaaaccgg ttttcgggag
    18181 atttctttat ttaagttgta aatagggtga tgtcctgttt tctagatgat tctgaattat
    18241 ttttaaaaga agcattccaa agttgaaaaa agcagtagtt gctagtatga ttcactatga
    18301 gtttaaaaac ggaaaaagga gtacataaca aatgaaagat tatttggtta aggcacttgc
    18361 tcacgacgga ttcgtccgag catacgccgt tcaagcaaca aataccgtag cggaggcaca
    18421 aagacgtcat gatacttggc acacctcctc agctgctttg ggacggacat tggtcggtag
    18481 tctgctactt ggtgcgacgc tcaaaggaga agacaaacta actgttcgaa tccaaggcga
    18541 tggtccagca acaggaattg tagtagatgc aaatggaaag ggagatgtga aaggttacat
    18601 caaaaatccg catctcagtc ttcctctaaa tagtgaaggt aaaatcgatg tgcggggagc
    18661 agttggaaca caaggaatgt ttactgtcac aaaagactta ggattaaaag aacctttttc
    18721 tgggcaaacg ccgattgttt ctggagaaat cgctgaagat tttacttact atcttgctat
    18781 ctcagaacaa atcccttcag ctgttggact gagtgtattg gtcaatactg acgacagcat
    18841 caagacggcg ggaggattca tgatccaaat catgccaggt gccgatgaag aaacaatcag
    18901 caagatcgaa gaacaattaa aaacgacacc aagaatctct accctcttag atgaaggaca
    18961 gacaccagaa gaaatattga aaggattgct tggtacagaa gaattgaaca ttttggaaaa
    19021 aatgcctgtt caattcaaat gtgactgttc aaaagaaaaa tttgcagaag caattatcgc
    19081 gttaggtgca gaagaaatcc aagcaatgat agacgaagat cacggtgcag aagcggtttg
    19141 ttctttctgt aataacaaat acaagtatag cgaagaagaa ttagatggat tgaagaaaga
    19201 agccttagga gggatttaag tggaacacac atggaaaatt ggcaatgtgg aaatccctaa
    19261 tcgtgtcgtt gttgcaccga tggctggaat aacaaattca gcatttcgag tcactgtcaa
    19321 agaatttggt gcgggcttag tcgtatgtga aatgatcagt gaccaaggta ttcatttcag
    19381 aaacaaaaaa actttagaga tgctccacat tgaagaaaca gaacatccat taagtttaca
    19441 gatatttggc ggaaataaag atagtctagt tgaagctgct caatttgtcg aagaaaacac
    19501 agctgccgat ataatagata tcaatatggg ctgtccagta aataaagtaa tcaaagcaga
    19561 agcaggagca aaatggcttt tagatcctga gaaagtatac gaaatggtcc aagctgtttc
    19621 ttcagcagta aatatcccag taacaatcaa gatgcgtatt ggttgggatg aagagcacgt
    19681 ctttgctgta gaaaatgcat tagcagcaca atcagctgga gcaagtgcca tcgctatgca
    19741 tggacgtaca cgggttcaga tgtatgaagg aaaagcggac tgggaagtat tgaaagaagt
    19801 gaaaaaacat ttgacgattc catttatggg gaacggggat gtcaaaacgc cagaagatgc
    19861 aaaatggatg cttgactatg tcggtgcaga cggtgtgatg attggtcgtg cagcgctggg
    19921 caatccatgg atgattcacc gcacacagca ttatctcgaa acaggggaac tgattcctga
    19981 accaacacca gccgaaaaaa ttgctacagc taaacttcat ttacaacgct tagcagatct
    20041 caaaggagaa aaagtagctt gtagagaatt cagaacacat gcggcttatt atttaaaagg
    20101 gattcctcga gcagcgaaag tgaaagtagc aatcaatcag acagaaaaac agcaagaaat
    20161 caatgacctg ctggatcaat ttgtagaaaa agcggaaaaa gtactattga aacaaacgtt
    20221 gtgatttcta agtttttcgt gaattttcaa gccgaaattc caagttcggc ttgctttttt
    20281 tgtgtttcat tggcattata ggaaagagat taacatgaga atggaggaac ttttgtgaca
    20341 caggaacaac aacatcaagt agaagattta aatgatcaaa tgcttgtacg tcgtcaaaaa
    20401 atggaacaat tgcgtgaaga tggaatcgat ccatttggaa aacgatttga acgcacgcac
    20461 aactcagaag aattgcatga actatttgat ccaagaacaa aagaagaact agcagaaatg
    20521 ggattgaccg ctagtgtagc tggtcgtatg atgactcgcc gaggaaaagg gaaagctgga
    20581 tttgcgcatc tacaagatcg tgaaggccaa attcaaattt atgtaagaaa agaccaagta
    20641 ggtgatgagg cctatgaagt attcaaacat gcagaccttg gagatttctt tggtgtaact
    20701 ggacaaatca tgaaaacaga cacaggcgaa gtttctatca aagcttcaga aatcacgatt
    20761 ttgtcaaaag cacttcgtcc actgccagat aaataccatg gcttaacgaa tattgaacaa
    20821 cgctacagac aacgctactt ggatttaatc agtaatcgcg aaagctttga ccgatttatg
    20881 aaacgaagcc aaattatcag cgaaatccgt cgctatttag atggcaatgg ctatattgaa
    20941 gtagaaacac cagtattgca caatgaggca ggtggagctg ccgcacgccc atttatcacg
    21001 catcataatg cactggacat tgatttatat ttgcgtattg cgctagaact tcatttaaaa
    21061 cgactaattg ttggcggaat ggaaaaagtt tatgaaatcg gccgcgtttt ccgtaatgaa
    21121 ggaattgata ctacacataa tccagaattt acgatgttag aagcatacac agcatatact
    21181 gatttcaatg atgtaatgaa tctgacagaa ggtattatcc gtaacgcagc tgaaaaagta
    21241 ctaggaacag caaaaatcac atatgatgga caagctgtcg acttagaaag tgactttaaa
    21301 cgcatccata tggtagatgc aatcaaagaa caaactggtg tagacttctg gcaagaaatg
    21361 acattggaag aagcattagc tttagccgaa aaacacaatg tagaagtaac cgaagctatg
    21421 ggtgtcggac atgttatcaa tgaattcttt gaaacatttg ttgaagaaac attgacacaa
    21481 ccgacgtttg tatacggaca tccagtcgaa gtttcgcctt tagcgaagaa aaatccagaa
    21541 gatcctcgat tcacagatcg attcgaacta ttcatagtgg ggcgcgaatt tgctaatgcc
    21601 tttacagaac tgaacgatcc aattgatcaa agagaaagat tcgaagcgca agaaaaagaa
    21661 cgtgaactag gaaatgacga agctcacggt gtagacgaag acttcttgga agccttagaa
    21721 tacggtatgc cgccaactgg tggtctaggc atcgggatcg acagattagt aatgttgcta
    21781 actgatgcac aatcaattcg ggatgttttg ctattcccaa caatgagata atcgaataaa
    21841 agctgtctaa ggaattcctg gctcttcgtc aaattgtgtt ggcgaagaca aatttgaata
    21901 aaacttagtc cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
    21961 cagaccttgt tgtaaataga tccgtgcccg aaagttataa aagttgcgat aaccaaacgc
    22021 aattcgttta atcagcttga tcttgttatt cagcccctca gtgacgccgt ttgagtgggg
    22081 caccttaaag gcatttgaaa tacccgattg gtagcgatgg aagatcgtga atttcttttt
    22141 aaacgactga ggtaacgtag atgaaattgt tttaagggtt tcttgaaagc cttccgtatc
    22201 tcgtttaacg aaataatagc gtaataattg acaaaaatga tacgcagttc gcaattcttc
    22261 gttgtagctt aacagctcat caacgatttc tgtttgacta attggtcgtt taaacagtcg
    22321 atgataatgc cgtttgagtg gctccaatgt cgtggaatct ttgagtaaga gtttccaata
    22381 tcgcttgagc cgacggtact tcttctgatt ttcagagtgt gaggttcgaa aacgattcat
    22441 gatctggaca cgtaattgat tgaagctgcg attgatgtgt tggacaatat gaaagcggtc
    22501 agttacgatc tccgcatttg gaaagactgt tttaagcaac tgacaatagc tggcattcat
    22561 gtccatcact aggtacttga cctttaaacg ggcctttcta ggaaagcgca ggaagtattg
    22621 agttagtttg taaagtctgc gatcttcaag gatcgtaaat agctgattgg ttgtcccatc
    22681 aacacaaata aaactcatgg aaccagaaac agacttcatt gatttgaact catccataca
    22741 caacaccgtc ggaaggaagt tcatacgtgg ctggtaatgc ttcgttgctt cacgcaagat
    22801 ccgtgtcacc gtcccatccg agacgaagta cttcttagcg attagtttac gtgattgatt
    22861 ttcgtacagt tccatgagaa tcgcctgctt cagttccttt gttagttgat gatgttcatc
    22921 aactaagtcc gttttcgcag aaaaggtccg tccacaatca tggcagagaa aacgggtacg
    22981 ttttaattct aaaatagtca acttttcttt gactttcaac atctgagtgc gtgtcgtata
    23041 ggaaccgttg cggataatac gaatcgatcc acagtgttca caatgggaag gtgagtcatc
    23101 caatctccct ttaataaact gcgcttgaac gcctttatat tctttttcaa tcagccattc
    23161 ttcatcaaaa aataaatgtt tatctgttaa tccgagtaat tttctagtat gattatccat
    23221 tgagagactc ctttggtagg gtgacgtttt ggtcgactaa attttaccaa aagtgagtct
    23281 ctttttgagt tgaaacgaag aaaaaagtgt tagtagaaaa tcgctttcga ttttccacca
    23341 acacaaaata ttatagaacc aacaaaaaaa gcgagacata agtcttcgct ttttttgttt
    23401 tcttttattt tgatgaatca gttgtttctt cagttgaaga ttcttttgtt gaatcagttg
    23461 attttgtgtc agaactcttt gttgaagctg tagtttcact tgagtctttc gtacttgatg
    23521 aagttgttgt gaatgcactc aacacatttt caaaggcatc atctttgatt tttacatttg
    23581 catcttttaa ttcttcaccg attactttag ttgtgaatgc attgtcactt agtttagttt
    23641 cagtagcgat atcttttagt tgatctttgt atttatccat gtcattgcct ttattttggt
    23701 ttttcaccat tttgacaacg tagtattctg tcgcataaga agtaggattt gttgtagtaa
    23761 taacatcgga gatctcaccg tctttcaatt tgaaagctgc ttcttttact tctgcaggaa
    23821 tagttgttgt cgttgagtcg aatttaactt taccgccatc ttcttttgtc tctgtatcag
    23881 ttgatttgtc tttggctaat ttagagaaat catctccatc atccgctgat tttttcacat
    23941 cttttgcttc atcttcactt gataatttga tgatttgtgc ttctacttct ggatggaaag
    24001 atttccaagc agttttcaaa tcgtcatctg taatatctac gtgagctttc aagccagctt
    24061 caaaagctaa attgttacga atatattctt tataagtatc tttcgtatat ccagcagctt
    24121 ctaattgtga ttcaaaagta tcacctaatg atttcgcttg tttgtcgtat tctgcatcca
    24181 cttgcttatc ggttacttta tcgccatatt tgttattgaa tactttataa atgatcatcc
    24241 gttgtactaa ggattggttc gatgattcaa gttttgcttc atcatagaaa tcagatacag
    24301 tgatcgttcc gcctttcatc gttgcgatat ctttattagt atcgcctgaa caagctgcta
    24361 gagttaaaac agccaacgca ctagtagctg ccaaaataat tgattttttt ttcattaaaa
    24421 tttgcactcc tgtaattagt tattattttt atatattaac tataccatat cccatttcct
    24481 ttaaaaacgt tgtcgtggta gtttcaaaca aaattcaaaa tttcatcaca ttcctaggaa
    24541 atacgcaaac ttttctcact tatatagtta aatctgttga gaaaataatc attattatat
    24601 ttgttcagga tactctggca gatcagcttt tagtttttct acttgttcat tgatttgtgt
    24661 gatacgtggt tcggcttgga atttaaaatc ttcgatatct tttttgattg cttcttggaa
    24721 agcaggaatc acagattcag ctgtttcttt tgtttcgatc aatgcgcttt taaaatgatt
    24781 caggctgtta tttaaatcat ttgtcagagt tgttgcttct tccaattctt cgatcagttt
    24841 ctttcttgtt tcattcccgc tcctgggcgc caataacaga cctccgacag tgccagcagc
    24901 agctccaaat aacagtcctt tgaaaaagtt tttaatcatc gcaggacacc tgctttacga
    24961 ttgtttcttg aatagctttt agttcttccg atccataact atcttgatga ttgacaaagt
    25021 gaatagaaaa atcatcctct ttgctgtagc gagggactaa atggatatgt gaatgaaaaa
    25081 cagattgata ggctagttca cggttgttat tcaagatatt aagacctttc atttctggaa
    25141 aagctttttc caatgcttga gcaatcttag gcacacgcga aaaaacctct cctgcaagtt
    25201 cgggatcata ttcaaaaata tctgctacat gacgtttagg cacaactaat gtgtgtcctt
    25261 tcgttgcttg agagatgtct aaaaaagcat agaccttgtc gtcttcatat attttatagc
    25321 ttgggatttc ttgattgatg attttgcaaa aaatacagtt ttccatataa aacatccttt
    25381 ctataaagaa taaatacttc agcttaactt taccataagt taaagctatt gtaaggaaaa
    25441 aaacaaaata ccgtcatttt cttcgtggta taataaataa aaataaatgg cggaggaaaa
    25501 agaatgagtt taattatcga tcatgtcaca ggggggtacg gacatctccc tgtgttaaaa
    25561 gatatcagtt tcgatgtaca gcctggagag atagtagggt tgattggact gaacggagca
    25621 gggaaaagta cgaccatcaa gcacattatc ggtctgctgg aagcttcaaa aggaacgata
    25681 gaaatcaatc atctatcctt aaagaaaaat acggaagaat atcgcaaaca aattgggtat
    25741 attccagaga ctccttcttt atatgaagaa ttgacattga gagaacatat cgaagtcact
    25801 gcgatggcct acgatatacc tatggatata gcaatgaaaa gagcagaaaa gttgttggag
    25861 acttttcggt tatctaatcg tcttgactgg ttcccagcta atttttcaaa gggaatgaaa
    25921 cagaaagtga tgatcttatg cgcctttttg atcgagcctt ctttatatat catcgacgaa
    25981 ccatttttag gcttggatcc actagctatc aatgcattgc ttgaattgat ggtagaaatg
    26041 aaaaaagaag gagccggtat tttgatgtct acgcatattt tagctactgc agaaaaatat
    26101 tgtgatcgtt ttgtggtctt acataacgga gagatacgag cagacggttc tcttgagatg
    26161 ttgagaaaag aatttcagct gccggattct tctttggacg aaatctatat tgctttaaca
    26221 aaggagcagg cacatgagta gtttttttca aaaacgttta gcaagacatc aaaagaaaat
    26281 gatgaaatat atgcgttatg tattgaacga tcattttgtt ttagtttgtc ttttcttgat
    26341 tggcggtgtg ggattatatt attctaattg gttgaaaacc ctgcagccac cgtttgcaat
    26401 tggagggctc ataatctctg tattttggat gctctgttta ttcgtgggta agatggctac
    26461 ttttgcagag gcagcagata ttgttttttt acttcctaaa gaaaaagaaa tgcaaaccta
    26521 tttggaacga gcttttcgct attcttgcat ttttccgttg atctttttga tattggcatg
    26581 cggattggcc atgccattag tggtggttag tacggagcaa tccttttcga tgtttttcgt
    26641 ctatctgatc atattatgga gtttgaaatt cagtcatctg caaatcaaga gagcagaatt
    26701 gtttcgacta gatcaacaag ttattcgtta ttggaaatta ggctggtttt tttcttctct
    26761 gatctttttg tttctatcat tttattggct aatatgggca ggaatagcag gtgcattagt
    26821 acaggcaagt ctatattact ggtttttgtg gaagcaacaa gaaaaaggat tggattggga
    26881 gaaaatggtg catgtagaag aacataggtt gcatcagatc tatcaattta tcaatttatt
    26941 cacagatgtt cctgaaatta cggcaaagat caaaagaaga cgctatttgg atcctatatt
    27001 gactcatatt aaaagagaat caaaaaacac ttatctttac ttgtttgcaa gaagattggc
    27061 aagaggttct gattttagtg gtctttattt ccgattagta attataggtg ggttattgat
    27121 tgccggagta gtcgactttt attttagtct gattatcggt ttcttatttt tatatttgat
    27181 cggtttccaa ctgttacctt tagcgaacca gttccgctat atggttctcg ttcagcttta
    27241 tccaatccat aagaatcaga agaagcaggc gattcagtat ctactcacat gggtattaat
    27301 tatcgcgtcg gttatttttg gtttgattgc tgcagtcgta ttagatgggc agcagcgatg
    27361 ggttccgcta gcggcttatt tgattgttac tatgctcttt gttaaactat atgtaccaac
    27421 tcgtttgaag aagatgtcag actgaaaaca aagctgtgta gggtaaaaat gagaatgaaa
    27481 aatagagcgt tgacagtaag aatgctgttc gttctatttt ttttgagaaa agctggtttt
    27541 ttgaatggca attaaatgaa aaagaacgta tatgtgcttg acgtgtagtg ttcaggcgac
    27601 tagaataata agtactgcaa aacaaagagt aagaggacgg gagaatgcat gatggagttt
    27661 cagttagacc aagagtggcg gttacgtccc atcaaaggcg acacaggaaa aacatacata
    27721 ggcatgcgtg atgaggacaa agtctttatt aaacgaaaca cgactccgat gttggctgct
    27781 ctttcaaggg aaggaattac accaaaactt gtttggacaa aacgaacggg gaatggcgat
    27841 actttgacag cacaagagtg gctggaaggt cgcttgctga ctcctaatga gattggtcaa
    27901 agaaacgacg tgattgatgt tttgtaccat ttacatcatt caaattctct taaaaccatg
    27961 ctgacccgta tcggtggtcg tgtcatgacg ccggagaaga tgctgcaaaa atatgaaaaa
    28021 gaattaccgg aagaactcca gaaaaatcaa tttctgcaaa ttgtccatcg ttttcttcgt
    28081 gagcagatcc cgtcaataga taaggaaaac ttatcagtag tacacggaga tgtaaaccat
    28141 cgtaattggt tagtatgtaa aaattattta tatctagtcg attgggattc aatcatgttt
    28201 gcagaccctg cggtggatat tgcaacgatt ttaggaaatt atgtgccact gtctagctgg
    28261 aatcagtggc tgatcagtta tggcatacgc ccgactgacg aaatgcttga aaaatttcat
    28321 tggtatgcgg ccatgaattt gctccaagaa atcaccagat attgcaaacg tgaagatcat
    28381 cgacggatga acgaagaaat tttacaatta aaacgaattt tcagtggctg aggtttggcc
    28441 caaactctgc ggagatttgg ccagaccttt ttaattagag gaaaggaatg gagataaata
    28501 atgcgtgtcc gaaatcgtcc gggagcagcg gaaatgttag cggctcaccc gaattttgta
    28561 attagtgatc caacattgtg gaagggaaaa tggaatgaat tgtttgaaaa cgatcatcca
    28621 atccacattg agattggtat ggggaaaggc cagttcatca ctggaatggc aaaagcccat
    28681 cctgaaatca attatattgg agtagaaatg caggtcagtg ttgtttcgat tgcactagac
    28741 aagttgatcg aacaaccgct tcctaacctg aaacttctac atgtggatgg atcagcgttg
    28801 acagagtatt tcgctgacag cgaagtagat caaatctatc tgaatttttc cgatccatgg
    28861 ccaaaaaaac gtcatgaaaa acggcgattg acatataaga cttttcttgc tgtagatgaa
    28921 cagattcttc gtccaaatgg agaaatccat ttcaaaacag ataatcaagg attgtttgag
    28981 tattctctgg caagtttttc tcaatatggc atgatcctca aacaggtatg gttagattta
    29041 catcaaagtc aatttgaagg caatattatg acggagtatg aagaaaaatt ttcttcaaaa
    29101 ggtcaacgta tttatcgagt agaagctcaa tttcaagata agaccaaaaa gaaaagtctg
    29161 tgaaactaac tttctgcttt cataaaaaca ttaatatcca taaataaatc ccagaaaatg
    29221 aacattacag atgtaatgta ttttctggga tttatttgat ttaaaatgta tttatttcat
    29281 cggtttttcc acaaggtgga atcaattgga agcgagaaaa tgaaaataat cctttgtcgg
    29341 tgatcttcaa ttcgggtatg acagataatg ttaagaaaga aagtgtcaaa aaaggatcaa
    29401 aggttttagc tgctcctagt tgatgtgctt tagttattaa ttcattcaac tgttcataga
    29461 cgatcgaaaa aggttcctgt gtcatgattc cgcctatagg aagagaaaca caagctattt
    29521 cctgctgttt gttgacaacg gtcatcccgc cacctttttg aatcaaaaga ttagcagcat
    29581 acagcatatc ctcgtcgttt gtcccaacga caactagatt atgcgagtca tgggcaatcg
    29641 ttgtcgctat cgctccttgt ttcagctgaa atcctttcac tatgcctaat ccaatgtttc
    29701 ccgtttgatg gtgacgctcg atcaccgcaa tctttaattg gtctttttcg attgaaggaa
    29761 tgaatcgttc gccttgtcgg tccacttttt ctaccaaatc tttagtcact agactattcg
    29821 ggataatttc gatcacatgg gcttgtgaag aagaaagaag cagctctaac gaatgtggat
    29881 ttaatggttg agcgtttagt tttggcagac aacacgtata tgaatttgaa tgatcagcaa
    29941 ataaatggtc ttggagttgc ccagattgaa caactaatgt tcccttatag aaaacttcat
    30001 gaattggcag cttggtaaga tcgtctgtta agaagaaatc agcttgatac ccaggggcaa
    30061 tcgcacctag tgttttcaaa tcaaaacatt ctgctgcatt caatgttgcc atctgaatgg
    30121 cttgtaatgg atcgattcca agtgcaatag cttgacggac gatatgatcg attgatcctt
    30181 cttttactaa atcattgatc agtttatcat cggtgacaaa gaagcatctt cttgaatttt
    30241 caggtgtgat cgccggtagt agcgatgcca gatcttttgc aacggtccct tctcgaacca
    30301 ttaaatacat accaagatcc aaacgttctt tagcttcttt tgcagttgta gcttcatgat
    30361 ccgtccgaat acctgctgca ggataaacat ttaactcttc catgccgata ccagctgcat
    30421 gcccgtcaat ttttttctta tgctggtgca tcaattggat cttttctagg atgtctgttt
    30481 cattatttgc tactgcttga tagttcatga cttcagctaa gcctaaaact tcgggttttt
    30541 gtaaaaaagg ggctaatttt tttgcattta aatttgcacc attggtctca aaaggggtta
    30601 caggtacaca agaaggtagc ataaaaaaga tattcatgag tgtttgtctt gcatcttcta
    30661 tcataaactc aagacctgta ctcccagcaa tgtttcctat ttcatgggga tctgtcacta
    30721 cagtagtaac tccatgaagc aaggatactt ttgaaaattc gcttggtgat aaaagtgagc
    30781 tttcgatatg cacatggcca tcgatgaatc ctggcactag atattgccca ttagcattat
    30841 gtacgctttt tccttcgtat tcaccgattc ctacgatcat tccttcgcag attgctacat
    30901 ctttttccat gatttctttc gtaaagacgt tgacgatctt agcatgcttg atcactaggt
    30961 cagcaggctt ttttttactt gctacttcga tatatttcct taatgttgtt tccaatggag
    31021 tgcctcccaa gataaatttt tataagtata acataaattc ggtttttgcc aatatttttt
    31081 tgtaaattca ttttatttga aacaaaaaga tcaaaaagac gtatgttttt tgtggagggg
    31141 agaataatgg aaataaaacg attatcggat caaatgataa gtgaaagttt tgccaaacat
    31201 gcccaagacg tatatatatt gccagcttca gaagtatttt acatctattt tcgctatgga
    31261 aaaaaacgaa cattctggaa gaaagtctct tcggcagatg gacagcaact gatctcaaga
    31321 tttaagtatt tgggacagat ggatatcgga gaaaaaagaa aagctcagtt aggtgcattt
    31381 acttattctt tagaaaaaca ggaagttcgt ctgcggttgt cttctgtagg agattacctt
    31441 caacgagaga gtttagtgat tcggctatta tacggtactt ttgaaacacc ccatcgttgc
    31501 tttagttctc gagattttgc aactatttta cagaaaaccg tcagacgtgg attatacctt
    31561 ttcagcggac cagtcggatc aggcaagact tctctaatgt atcggttggc tttggaagaa
    31621 aaattgcaag tgattactat cgaagatcct gtagagatag aagaaccgtt ttttctgcag
    31681 ctacagatca atccgaaaat agcacagact tatgatacac ttttgagact cgcattacgg
    31741 catcgtccag acttgctaat cattggcgag ataagagatg aatcaactgc acatgctgcg
    31801 gtaagagcag cactaacagg gcaccgggtt tttgcaaccg tacatgcacg caatcttgaa
    31861 ggtacattgg tcagaatcca tgaactgaca aagaaaaaag aagagctatc tgaatgttta
    31921 gctggtgtca tttatcaaga acttttggaa gatgccaatc accatccttc tgttttatgg
    31981 agttatcaat ttatggagga agaatcagta aaaaaatcat gggaggaaag ttatgaagaa
    32041 gctcaggaaa atatttggca gtcagaacta ttctaaagga ttaacaagaa gccagcaaaa
    32101 ccagtttgca caaaccatgg gagaactgct tttatctggt tttagtctgc aagaatccct
    32161 ttatttatta ctcacgatcc gttcgtttcc acctgttatt ttggcaaata tccaacatca
    32221 gctaaaggaa gggcaagcgt ttagtgaggt actttcgcaa ataggatatg gagcagagca
    32281 actggtacag atcgagttag ctgaacgtca tgggaatctg ccgacaactt tgttggaaat
    32341 tgccaaacag tttcggcttg ttgaaggatt tcgcaatgat ttgaaaaagc tgcttgcata
    32401 tccgatgcta ttgctcacct ttttgctagg tatcttgttt agtatgcggt tgttcgtttt
    32461 gcctcagctt attggttcag gaatggtcat gcaagagcat tggggtattc agctgataca
    32521 agtctttcat tggtatgttc tactattcat cctagcggtt actctattgg ctttactagt
    32581 aaggtatcgt ctgatgaagt tgtctcctgt tgatcagagt gaatggataa gcagacaatt
    32641 attgattgga ccgatatatt ccttgtatca atcggcttat ttgtcactag agattggaaa
    32701 gctttttcat gaaggtttcg aattaaggca aatcatagtc tgcttaaagg aaacgaaaca
    32761 agaaagtctg attcagcatc ttgcctctaa gttgattcaa ggattggagg caggtatgtc
    32821 attggctgag cagttccaga attatcgttt tctatcagaa gaattcagta aaattgtttt
    32881 acagggagag gcaaagggaa gtttaggtaa agaactgatt ttttacagcg aatggacaag
    32941 aaaacagctt ttcaaacgtc ttcatcactg gatgcatttt attcagccaa tcattttttt
    33001 gggcgtagcg gctttgattt tattagttta tgccgctgtt ttattacctg tttacggaag
    33061 catagaggag gttttgccat gaaaaagaga aaaatatctt attatagcgg ttttacattg
    33121 atcgaaatgc taatcgtctt actgatcatc agtgtattag tgttgttgtt cgtccctaat
    33181 ttgtcgcgtt ataggaatca tgtagaccaa gaaagtcgtg aagcgatcat ccagttggta
    33241 gacacacaaa aagagctcta tgcgcttcaa aataatggaa gagttcctac tgtggaggaa
    33301 ttgttgaatg aaggctatat caaacgagaa catgcagaaa tctatcagcg gccttgaagg
    33361 taaggtttta gcatcaggat atacgttgtt tgaatcactc ttactgattt tattacttac
    33421 aacgatcctt tcttttccag ttctcgcatt ttctgcatgg aagaatgaat tggcagtcta
    33481 tcaatttttc ggccaatttg aaaaacgaat ctattctacg caaaaaatcg ctattgtgaa
    33541 tcagatccaa acaggctttt attggaatag tgaagagaat caaatcattt ttcatgttcc
    33601 gaatccagat aaaataaatt gggaagtatt agatattcct gatgagatta ccttgaaacg
    33661 ccatgcttct attacttttg ctgctagaac cgggaatgaa agttcactaa aagcttatca
    33721 attttattgg gaagaaaaag agcggacgat tacgtatcaa ttccagatag ggagtggtcg
    33781 ctatacaaaa agaattgaat aaaacaggat ttgttttgtt ggagtccctt acagcgctta
    33841 tgatcgcttc tgtctgtgtc tacttagtga gctttggcca gctgtttttg cttcaggcag
    33901 aaaagaaaag tggggaggaa ctgctattta ttcgaatgat ttatgaagat atcaagacgc
    33961 agcggatgta tcacaaaaga ccagagcgaa gggaagaaat gatcccatac gatatccaaa
    34021 ttgtcgataa taatggagta atcgaaaaag tgtttgtcaa tatggagaaa gaagcgatca
    34081 gtattgaaaa aacaagtaat tccagcattt acgctgattg aatgtctaat tggactgatg
    34141 atattgagca gtctattgct cacattcaat cttttgattc agcaaaccat ccaagtaagt
    34201 gaatttttgc agcgtaaaga tcaaaaagag tggctaatat ttttagcaca attagaagaa
    34261 gaactcaaga gcagtcacaa tgtctctgtg aaaaatcagc aactgcacta cacgatcgag
    34321 gataaacagt atatcatcga aagatatcag gcaatgatac gtaaaagaag aacttccggc
    34381 ggacatcaac ctatgttaac aagtatcact gagcttcaat tattggaaaa agaacacaca
    34441 atttcttttt atgtacactt tgaaaatgga gaagatggat atgccatatg gacaaaaaac
    34501 gatcagtaga aaatgttata agggctctat tttattttct gtcttgcttt tatttctttt
    34561 atgtacgttt ttattgatgg cagcattaga aagctaccgt ttgtcaatgg atcttaatat
    34621 acgaatcaaa aattattata tagcaaaaat aatgaagcag atggctttat cggaacagtc
    34681 tacgcttgcc gaaagttcgg aaggagtttt ttactacaca actggatctg taacatatca
    34741 gtgggtacaa cagtccctat tcttagaggt ggaagtctca ccgtttatct ttcggttcac
    34801 agaagaagta aaaaatgata cagatactag tacagaatga tggaatgttc gtgaaaacgc
    34861 cctcttttct gttataacac tgtttttaat aaaaaaatgt ttgccgtttc acaaataaaa
    34921 aacagtgaaa acgattgaaa ttttgtcaat tgttcgatag aattaaacag gtgaaaaaat
    34981 tgttataata tatctagaaa tgaggtgcac catgttacca gaaaaaatgg aacaagggtt
    35041 taatcaaaac ctggaagcta ttcagctttt acagaatgca ttgggaactt cctttttaga
    35101 agcctatgtc gaaaatgcag aaaatttaat agatgattat caagtacgtg tggtggatgg
    35161 agtgccgaca aaagagacaa cgcaacggat cacagctctt tatgaagagt tgaagaaact
    35221 ttctttcgag ccagaagaat ggcgcagact gtctcaatta cttttattga agggcagcca
    35281 gacagaacat ttgcaggcga accatcaact cactcctgat agtatcggtt ttttatttgt
    35341 ttttcttatt gaacaactat acacaaataa aaaagcaccg gttaagattc tggatattgc
    35401 ggcaggaatg ggcaacttgt tattaacagt tcttttaaac ctatcgaatg cggggtacca
    35461 aacagaagga attggtgtgg atattgatga tacattgttg gctgtcgcag cttctacaag
    35521 tgatttgaca caagcaaatg ttcaatactt tcatcaagac gggttgcagg aattattaat
    35581 agatcctgtc gattttgcaa tcagcgattt gccaatcggt tattatccga atgacgaaaa
    35641 agcaaaagag tttttaacaa gtaccgaaga aggccatagt tatgcacatc atttattgct
    35701 agagcaatca atgaaatatg tgaagccgga tggttttggt ttgtttttga tgccgagtgg
    35761 atttttagaa acggatcaaa gtgaagaaat caaaaaatgg tttaaagaag aaggttatct
    35821 tcaaggaatg attcaattgc cagatgaatt gttccgtaat aaacaatcgc aaaaaagtat
    35881 tttgatctta cagaaaaaag gaccacaagc acaacaagta aaagaagtct tgcttgtgaa
    35941 acttgcttca ttaaaagaac cagagaaggt gacggaattt ttcaacgagt ttaaaaactg
    36001 gaaatcatct gctctgtaat cattcaacga gaaggagagt catatgtcta aaacaattgc
    36061 aatcaatgct gggagttcaa gtttgaaatg gcaattatat caaatgccag aagaaaccgt
    36121 agttgcgaaa gggatcgtcg aacgtatcgg tttacaagat tctattttta ccatcaagta
    36181 cggcgataat caaaaattcg aacaaatcat ggatatcgaa aaccacgaag tagcagtaaa
    36241 aatgctgtta gatcagttga ttgaactgca aattttaagc tcttatgatg aaatcaccgg
    36301 agtagggcac cgtgttgttc acggaggaga aacttatggg gattcagtag tgatcgatga
    36361 gaaagtaatg gaccgcattg ctgaattagc agaatttgct cctttgcata atcctgcaaa
    36421 cttaatgggg atcaaagcgt ttaaaaagat tttacctgac attttgagtg tggctgtatt
    36481 cgatacatct ttccactcaa caatgccaaa gcaaaattat ttatatagct taccggtgtc
    36541 ttattatgaa gatttcaaag taagaaaata tggcttccat ggcacaagtc atcgttatgt
    36601 gtcggaacgt gcggcaaaat tattgaacaa accgatcgaa gaactaaaaa tcatcacttg
    36661 ccacttagga aatggtgtat cgatcacagc agtcgatgga ggtaaatcca tggatacttc
    36721 tatgggattc acaccattag ctggggtgac aatgggtaca cgttcaggtg atatcgatcc
    36781 agctgttctc ccatatttaa tggaaaaatt agagattgat attgatgaaa tgatcaatgt
    36841 tttgaacaag aaatctggtc tgctaggcct aacaggattg tctagtgata tgcgtgatct
    36901 tgaaaagaac tatgaaaaag aacatattcg tttagcttat gacattttcg tggaccgcat
    36961 ccgaaaatac atcggcggtt atgtgacaac gatgaatggt gttgatgcaa ttgtctttac
    37021 tgctggtatc ggcgagaatg acggacatgt acgtagtgaa atcatcaaag gtatgtcttg
    37081 gtttggttgt gaaatcgatc cagcgttgaa tgaattgcgt ggtgaagaag tagatatctc
    37141 tacaaaagaa tcaaaagtaa gagtattagt gatcccaaca gatgaggaac taatgattgc
    37201 ccgtgatgtg gagcgtctgc gcggctaagc caaagcattc gttaggataa aagatgaaac
    37261 gcatgcaaga gggaattctt gcatgtgttt tttttgcgga gaagaccgca aaatggtaat
    37321 ttaacttcaa tttcccttct tttttacagg atcttcttag ggaattttgc ttggaaaagt
    37381 ggtcaaaagg gaaaagactg gcattttatt tattgatttt caggaaaatc ccaatatttt
    37441 attttcattc atctttataa gggacattac atatgtaaaa agaaaaaaca tttttagatc
    37501 agcctcgtgg gctgtcgact ttattcttcg ctttatgttt agctagaaaa ccgatccgaa
    37561 acattcattg atcgaataat aaaaacttga ttttccacct gtactttgcc tgaatcgtga
    37621 aagcacaggt atgaaaatca agttttttgt tgcgaaaaaa cgtcctagtc aatcagttgg
    37681 ccgagcggct ggcggctgat ctgctcctga tcaataacga gtgcttcttg gaagtgttgc
    37741 tgcaaaaagt agcggtcatg gctgactgct aggagcgttc cttgatagtc ttgcagcatt
    37801 tcttcaattt cttctcgagc atagctgtcg aggtgattgg ttggttcatc caaaatcagg
    37861 acgttgattt tttctaaaaa gagtttcagc aacgccaaac ggattttttc accaccggat
    37921 aaatctttga tccgtttacc cacatcgctt tgataaaagc cacttttggc taattgccgt
    37981 cgagcgactt cttcgtttcc agtcaatgtg atggcgtaag ccagcaaacg gctatcggga
    38041 tcggcaaact gcagctgctg cggcaaataa ccgatttgca agctactacc taatttgatg
    38101 gtgccaccgt aaggcttttc ctcaccgagt aataatttga gtaaggttga ttttcctgca
    38161 ccattgtcgc cgatgatggc taaatgatct ccacgataga ctgcaaaact gctgtctttg
    38221 aatagtactt gatcagcgta ctgctggtgc aagttttgaa tgatgaaaac ttctttgcca
    38281 gattttccac cattagacaa gctttgcagc cgatttttcg ggggaggtgg cggtttgacg
    38341 agtgtcagtt tcgccaggcg gcgttccagt tctttggctt ttttgaagaa ggactcattg
    38401 tctccttcat gtgcccactg acgaaaacga cgaatcatga ctttcaaccg ctggacttct
    38461 ttttgctgca gttcgtaatt ttttgtcagc tcagccaagc gagcttcttt caagactcga
    38521 aaacggctgt agtttccggg atactcaatc aatgcacctt cttccagttc ccatgttttt
    38581 gtggtgactt gatcgagaaa ttgccgatca tgagaaacga ccagaaccga tttttttgta
    38641 ttctgtaaat agttttccaa ccagcgaata ctgttaagat ctaaatgatt ggtcggttca
    38701 tcgagtaaaa gtaaatcggc atcggctgct aaaattttgg ctaaagcgac tttaactcgt
    38761 tcgcctccgc taagttctgt taaccgtgct tcctgacggt gtggaatccc taagccttgc
    38821 aagatcgctg caatccggtc ttctaatacg taaccgccta gttcttcaaa gcgatgctgc
    38881 agctcaccgt aacgggccag acacttctct aagtttgtat ctggttctgt catcaatgct
    38941 tcgtagcgac tcatttgctg tttgaggtcc tgtatctcag cggcagaatg gagcaagtag
    39001 tggttgacgg tcgcttcgga aactgtaagt tcttgttcga ccgtcccgat ctttaagcct
    39061 tttttgcgac tgataacacc gctgtcgaca ccttctctgc cggtcaagat ttgtaaaaag
    39121 gttgatttgc cagttccgtt gataccgact aaggcaattt tttcaccagc ttcaatttga
    39181 caggataaat cctcaaataa tggattcccg ccgattgttt ttttaataga aattgcttgt
    39241 atgatcatga tctctctcct tgagaccact caaaaaggcc atggttatcc atggcctgtg
    39301 ttatttaaac tcagattatc atgagaaaaa taccttgaga tggccattga ttttacgtga
    39361 attcaactgt tttggacaaa cttgtcccgt caacagcaac tgtaaaacca attgcatggc
    39421 taaatgcgat atggtatcgt tggtaatttt cattacttgt tattttagtc atgcgcatca
    39481 tagtattttc cctccttcgt gtagttgtct tttccagtgt acctgaaagt gtcagaaagt
    39541 tcaactagcg ctgacattta cttaatattt atcgatgtgt aattctgtca gagagctttt
    39601 tttatctgac tgagggtaat gtctcgtgga ccacatcatt gtattctgag ctaaaccagc
    39661 gttcaggcga aatcatatgg tgtttgataa aaaactttgt ctacatgact tccttttgcc
    39721 atttgcttaa taccgttgca ttaaacagct cttctctttg gaaatctcac caacatcgcg
    39781 ttcaggagta tcgttgtggt tgcaatggca agcggtagcc tttcatcaat tcttgaatga
    39841 tttaccgaat gtagcacttg ccttctcgcg gtacccgaac ccgaaataaa attttagttt
    39901 ggcgataaaa tcggtatccg ccatcgatac ttcatatttt ttcgtatcag attcatcgga
    39961 aacttccaca ttgacgaacc aataagcttt ggcatgctta ggtcgtttta aaccacacca
    40021 gggcgactaa tcgccctggt gttcactttt ttttatttta cttgaactca ttgactgttt
    40081 tccctttatt ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt tgtttttatc
    40141 acggtttttt gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt gttaagcttt
    40201 ggtgtggtct ttagctggca attggttttc taaatcagtt cggacaacaa gcacatcgca
    40261 agctgcgttt ctaataacat attcagaaac agaaccaata aataaacgtt ctacagcatt
    40321 caatcctgtg gctcctaaca taatcaaatc tacttgatta tcttctggga tttctctggc
    40381 aataatttgt tttggtgagc catattctac gacagaggtt acattattca agccagcttt
    40441 cttcgcattg ctttcataat ctgccaatgt ttgctttgcc atttcagtag cttgttctgc
    40501 aagcattcca tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga catgtgctaa
    40561 tagaagttct ccgttatttc ggatagctac attgaccgcc tttttaaaag ctaattcagc
    40621 ttcatcagaa ccatctacgg caaccatgat ttttttgtat tgttgtaaca taagcattca
    40681 ctcctcaagt caatcgtatt gtactctttt attgtagaac actttaggga aaaagagaat
    40741 tatttactgc tttggttcag caattttttt tgcaaatcgg atactgaagg tggcagaaac
    40801 aataattacg atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa aagctgttaa
    40861 aatacccatt acgcctaaaa cagcatatgc agctccgtaa aattgtagga atccttgatt
    40921 tttatcattt ttgttgatca atacaaaaaa gatatcctgt ttattaaata aataatagct
    40981 aacgaataat aatactaaag tggcgataat cattaaaatc ctaatcatga acaaatcctc
    41041 caaaataagc aacaaataga aaaagcaatt tgccaagagc aaattgcttt attctttaaa
    41101 agatggtaat ctccgttaat gccgttgttt gtcctgtagt attgatcccg cgaattcaag
    41161 caggtgggtc ccatggtcgt agcttcgaac taccaaatga taaggcatgt taccagcagt
    41221 gacacaagac gggtcccaag atatcaataa aaatgttcgg tcaacacaca aagaggacaa
    41281 ttcgaacatc acagatttcc cacctctata gtagcatatg aatttaaaaa gagcaatgag
    41341 agagctcgca gaaacagaac cgttttcttg tctagttttt tggaggatgc tttttccact
    41401 cttttatacg gtaatactgt tgacccagag cctgttcata tttccctgta tcttttggct
    41461 gataatactg agcattcttc agtttatctg gtaaatattg ctgattgacc caagcctgat
    41521 caaaatgatg aggatactga taacccacgc cacggttgag tgatttcgca cctttgtaat
    41581 ggctatctct caaatgatca ggaacatctc cagctttgcc ctctcgaata tcagcaagtg
    41641 ctgcatctaa tgccatgtag gcagaattgg attttggcga caagcaaaga tcgacgacga
    41701 cgtctgctag tgggatacgt gcctcaggca atcccaattt ttcagcagct aatacagcgt
    41761 tcactgtacg agccgcagca gcaggattac ctaaacctat atcttcataa ccgataacca
    41821 tcagacgccg gcaaatactt gccagatcac ctgcctcaac taaacgggcg agataatgaa
    41881 gtgcagcatc cacatcactt ccacgaatcg atttttgaaa agccgaaata acgtcataat
    41941 gagcatctcc atttttgtca tgggtaagag cttttctttg gacacattct tcaatgatcg
    42001 acagtgtaag atgaatcctt ccttctttat ctgaaggagt cgatagtgta gccagttcca
    42061 gcccattcaa tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga agaagagcat
    42121 cttcgtctaa ctggattgct tgttgtccaa ggccgcgttc tttatccctt aaagcatgtt
    42181 cgactgccaa ctggatgtct tgttctgtta atggtttgac ttcaaagatc tgtgtacggc
    42241 tgcggatcgc tggattgatg gtaatataag gattctcagt agtcgctccg atcaagatga
    42301 ttcggccact ttctaaatga ggaagcagaa aatcctgttt tgttttatct aatcggtgta
    42361 cttcatccaa taagaggatc actgtaccac tcatttttgc ttcttccgct acaacttgta
    42421 gatccttttt tgaatctgta gcggcgttta gcatgcgaaa tgcataatta gtagagccgg
    42481 cgatcgcact tgcgatactc gtttttccag taccaggagg tccatataga atcatggaag
    42541 agagcatgcg agcatcgacc atgcggcgta tgatttttcc ttccccgact agatgctgct
    42601 gaccaacgac ttcatcgatc gttcgaggtc tcatgcgata agccaatggc tgttgcatag
    42661 gattttcctc cttcttgttt ttacgtccct taattatatc atatttagca agatgcctag
    42721 aaagaacagc cttctgttgt tttgttttca tagcttatcc aactaatatt gtgtatgata
    42781 aagtaggtga gaaaaatgac ggataaacaa aagatagatg aatttttttg gaataaaacg
    42841 acgccagaag tggcacggga tttattgggg atgtatttgg agcatgacac accagaagga
    42901 agattagctg gatatattgt agacgcggaa gcatacctag gtccagaaga cgaggccgcc
    42961 catagctatg gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa accaggtacg
    43021 atctaccttt acacgatgca tactcatcgg attttgaaca tcattactca gccagaaggg
    43081 attcctcaag gagtaatgat acgtgcaatc gaacccgccg caatgattga tcaaatgagt
    43141 aaaaatcgtg gcggaaaaac tggtccagat atcagcaacg gtccgggaaa attagtggaa
    43201 gcattagcaa ttcctcaaga actttatggt cagtctatag cagatagttc gttacgctta
    43261 gtttttgaga aaaaaaagac accaaaaaag atcaatgctt tgcctagaat cggtatccca
    43321 aacaaaggtg tatggacaga aaagccgttg cgttttgttg tgtcgggaaa tccttatatc
    43381 tctttacaaa gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga aaatgaaaaa
    43441 gaaggatcta ttaacatatt tagatgaaat aattgaaaaa caagtgacag actatgatgt
    43501 agctttagat tgggatacaa aaaatcatac gatcgagatc gttgttcgct tgtttgcaga
    43561 aaataaagca caatttgaga ttgatgatgc agaagggatc gtttcgcaag aaccaattat
    43621 cgaatttgaa gacggcgtac ttctttttaa tcctcagaaa tctgtttttg atgaacgcaa
    43681 ttatctagca gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag tagctgattc
    43741 tttagtggaa tatctaaatg aagtattagc tcaagggcaa agcgatttgc ttgatttttt
    43801 ggatgaagac gatgacgaag ctgtattcga gcttcactgg gataatcagc aactagcaga
    43861 attagtagaa gtaaaacagc aaaatgaagc agatacttat ttaccgtacc caagttatta
    43921 aggagtgaca gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg aagcagtcga
    43981 agcagttgct aatattttga tggaggcagg ggcgagtggt gtcgcgattg aagattcttt
    44041 agatgttgaa cattttcagt cggatcaata tggtgagatt ttagataaaa atgattttac
    44101 atcgctgaaa gaaggcgcac tagtcatggc ttattttccg gaaacgactt tcttaccgga
    44161 aatcttgcct tttatcaaag atcgtatcac acaattacct gagtttggtc tagctattgg
    44221 aaaaaataaa gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag cctggaagaa
    44281 atactatcat ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct gggaaaacta
    44341 ccagacagaa gatcctctag aaaaaattat cacactagat cctggtatgg catttggcac
    44401 aggcactcat cccacgactt ccttgacatt gacagcacta gaaacagtct tgcgtggtgg
    44461 tgaaacattg ctagatgtgg gaactgggtc gggcgtgcta agtattgcag caaaatatct
    44521 tggtgcaaaa gatgtctatg cctttgattt agacgaagta gccgttcgtt cggcgaaaga
    44581 aaatatggat atgaatgaaa tagccaaaga tgtccatgta tcagctaacg atcttttgac
    44641 aggaatcgag attgaaagtg atgtaatcgt tgcgaacatt ttagcagata tcatcttatt
    44701 gatgatccca gatgcttggc gtttactgaa gcaaacaggc acgctgatcg tttcaggaat
    44761 cattgaagag aagaagcaaa tggttctgga tgccatgtta gaacaagggt ttatcgtgga
    44821 tcaaattttc cagcaaaaag actggtatgc aattattttg aaaaaaccag aggtggaata
    44881 gatgcagcgg tattttttaa aagaatcata tgaatcaaaa gactattttg ttttagaagg
    44941 ggaaaactat caccacattg tacgggttat gaggatggaa cccgaggatg aagttttttt
    45001 ggcattcaat gatcaaatag ccattgttgc agaaatcacg gagattactg aagaggctgt
    45061 ttatctgaaa gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt cgattaccat
    45121 agcgagtggc tatcctaaag gggaaaagct ggattggatc gttcagaaat ccactgaatt
    45181 aggagcgcaa gcttttcttg gtttcccggc atcagcctct gtcgttaaat gggatgcaaa
    45241 aaaacgtatg aaacgacaac aacggcttga aaagattgct caagaggcag cggaacaatc
    45301 ccatcggcag atcgtgccat cagtagagtt attggaaacg aaacaagatt tatttgagcg
    45361 gctagatgat tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc aaggcgaaaa
    45421 atcaaatgca gccaaaattc ttagtgaaac aaaaccagat agtcgaatct tagctgtctt
    45481 tggaccagaa ggtggattat cccgcgatga agtcgattat tttgtagaac acaaagggat
    45541 actttgtgga ttaggaccaa gaatccttcg tacagaaaca gcacctcttt atttattaag
    45601 tgctgctagc tatcaatggg aattaatgaa tccttctttc agctaacgtt cttcattaat
    45661 aaaagagtat cgacagaaag aattgaaaaa atagtcaaca atttgtataa tagaaacaat
    45721 tatttataca atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg aacatttccg
    45781 taaaaaaacg tataactgca cggattttgg gacaattatc aaacagatat ttgtcccgat
    45841 tcccgtttta ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa gaaattttaa
    45901 cgggacccgg tgtcataaag ctcgttagtc aatacatggg tcccgaacat gtagcatttg
    45961 ttgagaaagc atgcgaatac gcgactgctg cacatgacgg acagtttaga aaatcaggcg
    46021 aaccctacat tattcatcct atccaagtcg caggtatatt agcagattta aaaatggatc
    46081 cccatacagt ggctacaggc ttcttacatg atgttgttga agatacagaa atcactttag
    46141 aagatctgag agaagaattt ggcgatgacg ttgctatgtt agtggacggc gtaaccaaat
    46201 tagggaaaat aaaatataaa tcccacgaag agcagctagc agaaaaccac cgaaagatgc
    46261 tgctagcaat ggctcaagat ttacgagtta tcatggtcaa attagctgat agactacata
    46321 acatgcgtac gttgaagcac ctgcgagaag ataagcaaag aaggatcgct caggaaactt
    46381 tagaaatcta tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa tgggaattag
    46441 aagatacagc acttcgttat ctaaatccaa aacagtatta ccgtatcgtc cacttgatgc
    46501 agacgaagag agaagaacgt gaaaaatacg taagtggtac tgttgaagat attcgaatag
    46561 cgacagaaga gctggggatt tttgcagaaa tctatggacg gccaaaacac atttattcaa
    46621 tctatcgtaa aatgaaagat cagaaaaagc agttcaacga aatttatgac ttgctagcta
    46681 ttcgagtgat cgtagattca atcaaggatt gttatgctgt attaggagca atccacacaa
    46741 aatggaaacc aatgcccggc agatttaagg attatattgc catgccaaaa gctaatatgt
    46801 accaatcttt gcatactacc gtgatcggtc cggcaggaaa tccggtagaa attcaaatcc
    46861 gaacacagga aatgcatgaa atcgctgaat tcggggttgc tgcacactgg gcctataaag
    46921 aaggaaaaaa tgaaaaagta gaaccagatg gtatgacgaa acaattaagc tggttccatg
    46981 agatactcga acttcaagac gaaagctatg atgcttctga atttatggaa ggcgtaaaag
    47041 gagatatctt tagtgataaa gtctacgtct tcacaccaaa aggagacgtt actgagttac
    47101 caaaaggatc cggaccattg gactttgcat acagtatcca tacagatatc ggtaacaaaa
    47161 ccactggtgc aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg aaaaacggag
    47221 atatcattga gatcatgact tctccaaatt catttggccc aagtcgcgac tggttgaaat
    47281 tagttgctac tagcaaagca agaaataaga tcaaacgttt cttcaaagcc caagatcgag
    47341 aagaaaatgt gatcaaaggc cacgaatccg tggtcaaatg tattacagat ctaggattta
    47401 cgcctaaaga tattttgacg aagaacaaac tgcaagaagc actcgatcgt tttaattatc
    47461 aaacagaaga tgatctctat gcagctgtag ggtatggaga agttagcccc ttgacgatgg
    47521 ccaatcgtct gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa aagcaagaag
    47581 cagaagaaat catgaatcag ccgaaaaaag aacctgacaa aatgaaagta cgtcatgaag
    47641 gtggcgttgt cattcaaggg gtagaaaact tactaattcg tatcagtcgc tgctgtaatc
    47701 cgattcctgg tgatgatatc gttggttata tcactaaagg cagagggata tccattcatc
    47761 gtcgagattg tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt ttgattgaag
    47821 tcgaatggga agatacatcg aatacacgaa aagagtatga tgcagatttg gaaatttacg
    47881 gctataatcg ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg ctaacgaaaa
    47941 atctcaacag cgttgaagca cggacgaata aagataaaat ggcgacgatc catttgacgg
    48001 ttggtatcca gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa gcagtacctg
    48061 atgtctacag tgtacgccgg acgaatggat aggaggacag atatgagagc agttgttcaa
    48121 cgggtaagta aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat tggctacggc
    48181 ctacttgttt tattaggcat tcatgaaacc gatacgcaaa aagatgtgga ctatctggta
    48241 aaaaaaattg cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa tattagtgta
    48301 gaagatgtgg gaggacaaat tttaagtatc tctcaattca cactgtttgc ggatacgaaa
    48361 aaaggaaatc gaccaagctt tgtggaagcc gcacgaccag aaacagctat tcctttatac
    48421 gaatcattca atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg acaattcgga
    48481 gcagatatgt cggtttctct cgtcaatgat ggacctgtaa cgatcattat tgattctaaa
    48541 aacaaataaa gtaatctggt tttcactagt aaaaactatg aaacacgtcg cttaagttag
    48601 actgataaac tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct tttgtcagca
    48661 gtcggaaaaa aagttaagct taaattatac aagttatttt ttataggagg ttgctgaaga
    48721 tgaaagaagt accagtagta gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag
    48781 gatacaacat gttggaggag ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga
    48841 ttttcaataa agaagctatc acgatctacg gatcatcagc agcaaaagta ttttatgatc
    48901 caaggaattt caaacgaaaa ggtgctatgc caaaattagt attgaagaca ctttttggtc
    48961 aaggtggagt acagactttg gatggagctg ctcatcacca tcggaaaaat atttttatgg
    49021 atctaatgac accggaaaga atggaagatt atcatcgtat cttagataag aatctgacac
    49081 aagcacttga agcacagcat ggtcaatttg aactatttga tttaagtaag atggttttct
    49141 ttacatctat ttgcgagtgg gcaggtatca atttatcagc gatttctaaa gatgaggtcg
    49201 aaaaattagc agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc
    49261 acataaaagg tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag
    49321 aagctcgaca aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg
    49381 ccacagattt agatggtcag cttttacctc tggaagtggc agctgttgaa ttattgaaca
    49441 tcatccgtcc aacggttgct ctaactgtgt gggcggcatt gatgggacat gcacttttca
    49501 gtagaccaga tttgtatcag caattaaaaa atgacttttc gacgttacaa gatccgttta
    49561 ttcaagaaat gagacgctat tatcctttct tcccaatgct acctgcgatt tcgctgaaag
    49621 aagtagaagt agatggttat cgtataccag aaggcagttg ggttatttta gatttatatg
    49681 gaacagacca tgatgaacga acagttgaag cgccagattc ctttatgatc aaacggtatg
    49741 tagggaaagc aaaagatatt tcctataaag aagaatatga gatgattgca caaggcgggg
    49801 gaaattttcg acaaatgcat cgctgtgcag gagaatggat cactcttcat agtctacgtg
    49861 tgttttccga tcagctagta aataagtttg agttctctgt acctgaacaa gattggacga
    49921 ttccattcaa tcagtttccg acttatccga atagccgagc gctactttac aagaattaga
    49981 ttagcataaa cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata
    50041 ttactataaa ttgataaggt caattgatca aaatccacga aatgcttcat caatggtttg
    50101 ataatcttca ttggataaat gcaaatctaa agcttggaca ttgtcagata cttgctctgg
    50161 acgcttagct ccaggtataa ctacagacac tcgtggattc ttcatgtacc aagctaaaac
    50221 aagttgcgca ggagtggctt ggtatctttt agcaatgggg cgaagtacat cgacagcggt
    50281 taaaatttct ctgaaacgtt ctccttggaa atctggattt ctttttcgag gatctccttc
    50341 accaaattgt ttttcctcac caagttcata ttttcctgtc agcaaaccgg aagcaagagg
    50401 aaaataggga acaaaactga ttttgttttt ttcaagataa gggaataatt ctttttcagc
    50461 ctgccgatgg atcaaactgt atttatcttc tactacatcg acatatccat ctgcattcgc
    50521 ttctttcaat tgttctaacg tgaagtttga cacaccgact gctcggattt ttcctgcttc
    50581 ttttaattcg tgcaatgtag caactgattc attttttggt gtgctttcat ccggaaagtg
    50641 gatataaaaa atgtcgatgt aatcagtttg cagacgtttt agtgcatctt ccaccgattg
    50701 tttgagaaac tcaggagaat tatcaaatgt tcgcccttta tttggtacat gagcagcttt
    50761 tgtagcgatg acgacacgtg agcgatcata ttctttttcc ttcagtacct cgccaatcag
    50821 ttcttcggaa cggccgtttc cgtaagcata agcggtatcg atcaaattga tcccgctatt
    50881 caaagctgta cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg
    50941 tcctccaacg gcatttgctc ctaatccaag ctgttctgcg tagacttgtg aatgtccgat
    51001 tcttatttct gccatagctg attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa
    51061 gctgaactaa aacgtttttg tgaggggaaa tagtcagata tctcttgact ttgagactgg
    51121 tttttagtag gttataagta atatcgaatg tcaatgaaag agcgagtaag tttttgaaac
    51181 tttacaagag aggatcatcg aggctgaaaa tgatctaaag tttcattagc ctaaagacac
    51241 tctggagatg gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga
    51301 gaaatctttc aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcatgatga
    51361 acaagggtga tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa
    51421 agaccaaaag gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa
    51481 gaaacagcac gtttattatt cagagattat cagtataatg aaatgcgtac accgattttt
    51541 gaacactatg aagtgatatc ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag
    51601 atgtatgatt tttatgacaa aggggaacgc catgtgacgc ttcgaccaga aggtacagca
    51661 ccaatcgttc gttcttttgt tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc
    51721 aaaacttttt atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt
    51781 caattccatc aaatcggagt agaagcattt ggatcagaaa accctgcaac tgatgtggag
    51841 agcatggtga tggcacttga tttcttcaaa cagttgggga tcacgcaaat ccggctagtc
    51901 atcaattcat taggtgataa agaaacgcgt caagcctatc gtcaagcatt gatcgactat
    51961 cttttacctt ttgaatcaga gctgagtgaa gactctaaac gccgccttca tgaaaatcca
    52021 ttacgcgtat tggacagtaa agacaaacga gatcaaaaat ttgtggcaga agctccatct
    52081 atcttagatt atctaagcga gcctgctcaa aaacattttg ataccgttgt ttccatgctg
    52141 gatgcattgg ctgttcctta tgaaatcgac agtaatatgg ttcgcggact agattattac
    52201 acacatacga tttttgaaat catgagtgac gcaccaaaaa tgggggcaca agcaacgatt
    52261 tgcgcaggcg gacgttatga caatctagtg gaagaactag gtggtccttc aacacctggc
    52321 ttcggatttg caatgggtat cgagcgatta ctcttgacta tggaggcaga ggaagtagtt
    52381 atccctgcat ttaatgagtt ggatgcttac gtggtcgcac tcggagacga aacaaatatc
    52441 gaagcattga aagtggtcca agcgattcga aactttggtt tttcagctga ccgcgacttc
    52501 atgaaccgta aagcaaaagc gcaatttaag acagcagata aagcgaatgc caaattagta
    52561 ttggtgatcg gtgaagatga gcttgctagt caaacgatta atgtcaaagc aatggcaagc
    52621 cgaaaagaaa aaagttttcc attagaagag atatatgaac attttgatga agtctatgat
    52681 gaaatgactt tattcgagta ggaggaaaaa aataaatggc acaaagaacg atctattgcg
    52741 gattagtttc cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa
    52801 gacgagacct tgggggagta atctttatcg acctacgtga tcgtgaagga atcgttcaaa
    52861 tcgtctttaa tcctgaaatc aacaaagaag catgggaaat cgcggataaa tgtcgcagtg
    52921 aatacgtgat cgaagtgact ggggaagtta gaagacgcgg agaaaatgca gtaaatccta
    52981 aaatggccac tggtgaattc gaagtgattg caagtgaaat cgtgattttg aatcaagcaa
    53041 aaaccccgcc atttcaaatt gaagatgatc aagcaatcag tgacgaaata cgtttgaaat
    53101 atcgttattt ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga
    53161 caacaaaagc gattcgtcat tatttagatg ataatgactt tctcgatatc gaaacacctt
    53221 acctagggaa atctacacca gaaggcgcaa gagactacct agttccttca cgtgtccatg
    53281 ctggacattt ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg
    53341 ctggattcga ccgttattat caaatcgttc gttgtttccg cgatgaagat ttacgtggag
    53401 accgtcagcc tgaatttacg caagtagata ttgaaacaac gttcttaaca gcagaagaaa
    53461 tccaaactta cacagaggga ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag
    53521 tcacattgcc atttcctcgt atgacctatg atgaagcaat ggctcgctac ggcagcgaca
    53581 aaccggatac acgttttgat atggaattga tcgatctaag cgatacagtc aaagaagtag
    53641 aattcaaagt cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta
    53701 aaggcgctgc agatcgctac tcacgaaaag acatggatca gttaggtcaa tatgtaggac
    53761 aattcggagc taaggggctt gcatggctga aagtagaaga agacggattg aaaggtccaa
    53821 ttgctaaatt catgggagaa gcaacagaag caatcattaa agcaacagat gcaaaaccag
    53881 gtgacttgct gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta
    53941 tccggacacg tttaggaaaa gaattaggac taattgatga atcgaaattc aatttcttat
    54001 gggtaactga ttggccacaa tttgaatttt cagaagaaga aggtcgatat gtctctgctc
    54061 accatccatt cacgatgcca aaagaagaag atatcccgtt attggctgaa gaccctgcaa
    54121 aagtctatgc agaagcttat gatatcgtct tgaacggtta tgaattaggc ggaggttcta
    54181 tccgtattca tactcgtgag cttcaagaaa aagtactaga aactctaggc tttacaaaag
    54241 aacaaatgga agaacaattt ggtttcttgt taacagcttt agattacgga ttccctccac
    54301 atggcggtat tgcattagga ttggaccgtt ttgcaatgtt attagccgga gaagataata
    54361 ttcgtgaagt cattgccttt cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac
    54421 caagtatggt ttcgccttta caattgttcg aattgaatat tgatgtaacg aatatagaag
    54481 aatagccgat aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac aaccttgatt
    54541 taattgaaaa acactttata atgaagccat aacatataaa gcggggcttt tccatgaaaa
    54601 aaacattcga aaattgggct tatcatgatt acacgaccaa gctttcggta tctatcgttt
    54661 atgcgttgat cgcatcagtc gcattgaatt tcttttatca gccaggacat atttattcaa
    54721 gtggtattac tggtctagct cagattttga cgactttgtc aaaatcactg ataggattcg
    54781 aagtaccggt atcaacaact ttgtatttac tgaatatacc tttgtttgta ttggcgtggt
    54841 taaaaatcgg gaagaagttt acaattttca cttttatcac agtcactttg acctcccttt
    54901 gtatccatat tttaccggaa acagtcctat ctaccgatcc aatcatctgt gcgttgtttg
    54961 ggggggctgt gatgggacta ggtattgggc tagcgttgag aaacggccta tcatcgggcg
    55021 gtctagatat cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt ggttctattt
    55081 cgatctactt caatgcattg atcgtttttg ttgccggaat tctatttggc tggcaatata
    55141 tgttttacag tacgttgtct attttcgtca gtggaaaagt aactgatgtc gtttatacta
    55201 aacaaaagaa gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta gtaaatgaaa
    55261 tccaaaactg tatgcgtaga gggatcacga tcatcaatga agcagaaggt gcttataagc
    55321 atgataagca gacagtattg atgacgatcg ttacacgata cgaactgcct ctacttagag
    55381 aagcaatgaa agcttctgat ccacaagctt ttgtaagtat tgcagaaaac gtccagatac
    55441 ttggtcgttt ctatgaggat ggcatttaga tcgagtttga caaaaattca tgaaatactg
    55501 ctgtagacag acgttcgttt gtctgcagtt tttttctacc atagtagaat cagcaaagaa
    55561 aaactgcttt tttatttgaa attttcttaa agttgaaaaa aagcatgttt atttttttaa
    55621 atttatggga tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca
    55681 cttaataccc gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa
    55741 aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta
    55801 gctgtggtgg gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg
    55861 ggcgttccga tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa
    55921 tgggaacaaa atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc
    55981 tatccggcac ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga
    56041 gggctgttaa acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg
    56101 tggcaaatca ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg
    56161 catatctggt ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta
    56221 ttgttgatgg tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc
    56281 ttcctctccg ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt
    56341 tattacggta ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt
    56401 tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc
    56461 gctggaggaa tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac
    56521 aatttcatct attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca
    56581 gtgacagcac accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg
    56641 attggcaaac gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa
    56701 gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg
    56761 attctaatca tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat
    56821 tatggtcgta cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc
    56881 aaaccatggg ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg
    56941 gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt
    57001 aaaaaattag cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa
    57061 gcgatccttg ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa
    57121 ctaacggcat ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat
    57181 ccaaaagtag tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt
    57241 gaaaaattaa aggaacaaga tttgttgaaa gacactgttc tgattggact aggaacgaat
    57301 ggctcattct cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta
    57361 tactgggtca atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt
    57421 gaacagatga cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat
    57481 gaccatagtg actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat
    57541 tacattcact tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag
    57601 acaaacgtga cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg
    57661 aagttttttt atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt
    57721 gagtgtgtct gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct
    57781 gcttttgttg cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt
    57841 cttttttgaa gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta
    57901 gaaagatcgg ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa
    57961 taatgttcat ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt
    58021 gtaagtgatc aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc
    58081 aactaacaaa atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg
    58141 tgcaattcga tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac
    58201 catagtaaag cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa
    58261 cgtcgtatga atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt
    58321 tggcagccct ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac
    58381 gacagataag ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt
    58441 aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat
    58501 agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc
    58561 aacaatcttc ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga
    58621 aaaagaaaac actttggaac tttggataat cttgatatct tctgcgtata attggtcgat
    58681 tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta
    58741 tactattttt attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact
    58801 atgaccataa gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc
    58861 gagtactatt agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc
    58921 aagatgagaa ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg
    58981 cggtagctgc agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc
    59041 cagttctgcg atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag
    59101 gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca
    59161 tgtttcccag tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca
    59221 gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc
    59281 tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca
    59341 agaaaacgga taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca
    59401 aattggacaa agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata
    59461 agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg
    59521 aaaacgaggg agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag
    59581 acaggtgtca tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga
    59641 ttgaatgcgt tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga
    59701 cttgattgaa gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa
    59761 agtagaggca atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc
    59821 cgtagaatta tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt
    59881 tgctatatca ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag
    59941 ttattcgaac tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa
    60001 aaacgtacaa tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga
    60061 agatgacaat atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga
    60121 gctatttgat tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt
    60181 aaaatctgat tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact
    60241 acttacaccg tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca
    60301 ggataatgct cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa
    60361 aaaaggcgta tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga
    60421 acccctgcat gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt
    60481 ttatcagtcc gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat
    60541 cccgaatatg atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc
    60601 aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca
    60661 aaaattagca gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga
    60721 aaaatggctc ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc
    60781 gaaaaaatta caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg
    60841 tttgacagaa gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat
    60901 ttttgatggg atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa
    60961 agagatgcgt tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga
    61021 acatcagcag atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa
    61081 aggaaacgga acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg
    61141 aaaagaaata aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat
    61201 gaatctcgat gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa
    61261 ttttttggtt tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca
    61321 atttttccaa cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt
    61381 ggatcattta gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga
    61441 ggaaccagca gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact
    61501 cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca
    61561 actagaaaat tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat
    61621 attaataaac aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg
    61681 atggattggg tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga
    61741 aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc
    61801 gatgactacg cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca
    61861 aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt
    61921 cgaatcctta cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga
    61981 agaattgaat gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat
    62041 tcttcaagaa aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca
    62101 gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg
    62161 gtcgctcgca cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag
    62221 tcagctattg aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca
    62281 gcaaattgct cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc
    62341 aacggaacac cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa
    62401 agtagaacaa caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc
    62461 caaaagtgaa gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat
    62521 catggcaacg aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa
    62581 ggcatttcct gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc
    62641 tttagataga cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca
    62701 acaagcagaa aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa
    62761 taatcagctc cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac
    62821 ggatagtaac tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc
    62881 gaatcaacta gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct
    62941 tcggacacag cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa
    63001 ggaacaagaa agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga
    63061 aatctgtgca tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc
    63121 ttatcatgaa gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct
    63181 aactgagaca ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat
    63241 gatcgaaata aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa
    63301 aatctacgat gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac
    63361 gcaattacag cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga
    63421 acggtatgtg cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca
    63481 acgtttaacg aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg
    63541 gaaaaccgga ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca
    63601 taccctgtct ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga
    63661 tgtgatccag acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt
    63721 tgggtcatta gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag
    63781 tgaaggaaga atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca
    63841 acaaataaga attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata
    63901 aaaagggaga ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg
    63961 tcaatacttg caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg
    64021 gcatgcagaa gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga
    64081 gatagattac tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc
    64141 tgtggaactt tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat
    64201 gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct
    64261 aaaaacacaa aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac
    64321 gattgagact aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg
    64381 ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc
    64441 ttacacagaa aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg
    64501 ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca
    64561 tcaattatta ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct
    64621 tctcccagta gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca
    64681 tctgcagata aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt
    64741 atcattcaaa gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga
    64801 ttttttcttt atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa
    64861 atatcaacaa acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga
    64921 agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc
    64981 agagatcggc gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt
    65041 cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt
    65101 ctatggtgaa gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt
    65161 tcaggtgtta cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg
    65221 ctaccagaaa aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc
    65281 ctttttccgc acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa
    65341 attgcgagag ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac
    65401 cggttcatgg ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca
    65461 tgtcctgcaa agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct
    65521 ttcttttgat tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc
    65581 gatacgtaca gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa
    65641 tcaattggaa aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct
    65701 cattcatcca gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga
    65761 ttatcaaaag caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat
    65821 tttagccgat gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga
    65881 aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa
    65941 ctggcaggca gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa
    66001 taaaaaagaa cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc
    66061 cagcttaaga caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga
    66121 tgaggcacaa atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc
    66181 tgtacctcag cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg
    66241 gtcattgttt gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact
    66301 tgccgcagaa gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac
    66361 tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga
    66421 acaaaaaact gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga
    66481 ttctgaagca ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca
    66541 aatttgctgc gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga
    66601 acaagtaaaa gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc
    66661 tcaatttaca gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac
    66721 attttattta agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa
    66781 tgctggcgaa aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct
    66841 cacgggagca gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca
    66901 agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat
    66961 ctctgaagga acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt
    67021 ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg
    67081 catgatttta agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg
    67141 ggtaagaaag cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc
    67201 tgggattcca ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt
    67261 tatacaaaga ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg
    67321 caaattggag tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta
    67381 gcactttttg cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct
    67441 ggaagcatat gaaattcttt ctcagccagc tgaaaaatct ctttttctgg tttttggaaa
    67501 cctgttgctt gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc
    67561 ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc
    67621 ttttccttca agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt
    67681 tcttcctcgt aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct
    67741 aaatctttta gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct
    67801 gccattactt ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc
    67861 atatctgcat ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc
    67921 tgatcataca tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc
    67981 tttcggtttt cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta
    68041 aaaaagctga taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt
    68101 ttttatattt cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca
    68161 aattaatcat tatggtagaa gtcgattgat ttattttaat gcaatctctg tggaattttc
    68221 gaaagattta tttttttaat acagtctcaa tataactatt caaacataaa ataacgttat
    68281 ttgatttatt tgtttatagt aaattttatt tagtaatcat tgctttaaaa tactacgctt
    68341 atataacaga gaaaataagc gttataataa agaacagtat tatttaaaat gacagaaaac
    68401 attactatta aagaaataaa aaacataaaa aatcttaaga aatcggtttc atttagcttt
    68461 tgagataaaa taatagtaca gttttatacg ctgtttcata tgtttagaaa atttgaatta
    68521 gatttgaaaa ctctaatttt taaatgataa aaaaagcgag aatgctggtt ttactgtata
    68581 ttaaccttag ataactttca tttttttaaa aaaattatga aaaaaaggta gctatttaaa
    68641 agttgattat gtataataga aacagatggg agattattat gatcttctgt tacaaaaaag
    68701 tgattcttat cacgaaaagg agtggtgcac gttgttaaca ttatatactt ccccaagttg
    68761 tacttcctgc agaaaggctc gtgcgtggtt gcaagaacat cagattccat ttgttgaacg
    68821 taatattttt tcagaaccat tgaacagttc tgaattaaaa gcgattttac aaatgacaga
    68881 agacggaaca gaagaaatca tctcgacacg ttccaaagta ttccaaaaac tgaacatgga
    68941 tttagatgat ctgccgttac aagaattatt ggaacttgtg caaaacaatc ctggattgct
    69001 tcgccgtcca atcatgattg atgataaacg tctgcaagtt ggttttaatg aagatgaaat
    69061 tcgtcggttc ctaccgcgcg atgtacgaca attagaatta cgtcaagcac aattgatggc
    69121 tggattatag aaggataata cgaaacttgg aaaagac
//
LOCUS       DAYYBH010000008        61712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000008 DAYYBH010000000
VERSION     DAYYBH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.008"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHAUG_000585"
     CDS             complement(132..509)
                     /locus_tag="ACHAUG_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479702.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHAUG_000586"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHAUG_000586"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8479703.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHAUG_000587"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHAUG_000587"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8479704.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHAUG_000588"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHAUG_000588"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8479705.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHAUG_000589"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHAUG_000589"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8479706.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHAUG_000590"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHAUG_000590"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8479707.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHAUG_000591"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHAUG_000591"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8479708.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHAUG_000592"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHAUG_000592"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8479709.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHAUG_000593"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHAUG_000593"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8479710.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHAUG_000594"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHAUG_000594"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8479711.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHAUG_000595"
     CDS             10661..11575
                     /locus_tag="ACHAUG_000595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8479712.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHAUG_000596"
     CDS             complement(11708..13273)
                     /locus_tag="ACHAUG_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8479713.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHAUG_000597"
     CDS             complement(13266..14717)
                     /locus_tag="ACHAUG_000597"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8479714.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHAUG_000598"
     CDS             complement(14731..15747)
                     /locus_tag="ACHAUG_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8479715.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHAUG_000599"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHAUG_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8479716.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHAUG_000600"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHAUG_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8479717.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHAUG_000601"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHAUG_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8479718.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHAUG_000602"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHAUG_000602"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8479719.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACHAUG_000603"
     CDS             21340..22764
                     /locus_tag="ACHAUG_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8479720.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACHAUG_000604"
     CDS             complement(22806..23063)
                     /locus_tag="ACHAUG_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479721.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHAUG_000605"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHAUG_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8479722.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACHAUG_000606"
     CDS             24337..25314
                     /locus_tag="ACHAUG_000606"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8479723.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="ACHAUG_000607"
     CDS             25339..26982
                     /locus_tag="ACHAUG_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8479724.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHAUG_000608"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHAUG_000608"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8479725.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="ACHAUG_000609"
     CDS             28558..28833
                     /locus_tag="ACHAUG_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8479726.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="ACHAUG_000610"
     CDS             28839..29606
                     /locus_tag="ACHAUG_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8479727.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="ACHAUG_000611"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="ACHAUG_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8479728.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31864..33636
                     /gene="recQ"
                     /locus_tag="ACHAUG_000612"
     CDS             31864..33636
                     /gene="recQ"
                     /locus_tag="ACHAUG_000612"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8479729.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33750..34052
                     /locus_tag="ACHAUG_000613"
     CDS             33750..34052
                     /locus_tag="ACHAUG_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8479730.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34055..35218
                     /locus_tag="ACHAUG_000614"
     CDS             34055..35218
                     /locus_tag="ACHAUG_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8479731.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35244..38672
                     /locus_tag="ACHAUG_000615"
     CDS             35244..38672
                     /locus_tag="ACHAUG_000615"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8479732.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38693..39817
                     /locus_tag="ACHAUG_000616"
     CDS             38693..39817
                     /locus_tag="ACHAUG_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8479733.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39798..40244
                     /locus_tag="ACHAUG_000617"
     CDS             39798..40244
                     /locus_tag="ACHAUG_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8479734.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40228..40566
                     /locus_tag="ACHAUG_000618"
     CDS             40228..40566
                     /locus_tag="ACHAUG_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8479735.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40685..41245
                     /gene="rsmD"
                     /locus_tag="ACHAUG_000619"
     CDS             40685..41245
                     /gene="rsmD"
                     /locus_tag="ACHAUG_000619"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8479736.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41242..41733
                     /gene="coaD"
                     /locus_tag="ACHAUG_000620"
     CDS             41242..41733
                     /gene="coaD"
                     /locus_tag="ACHAUG_000620"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8479737.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41723..42766
                     /locus_tag="ACHAUG_000621"
     CDS             41723..42766
                     /locus_tag="ACHAUG_000621"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8479738.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42925..43653
                     /locus_tag="ACHAUG_000622"
     CDS             42925..43653
                     /locus_tag="ACHAUG_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8479739.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43769..44014
                     /locus_tag="ACHAUG_000623"
     CDS             43769..44014
                     /locus_tag="ACHAUG_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8479740.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44011..44229
                     /locus_tag="ACHAUG_000624"
     CDS             44011..44229
                     /locus_tag="ACHAUG_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8479741.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44365..44904
                     /locus_tag="ACHAUG_000625"
     CDS             44365..44904
                     /locus_tag="ACHAUG_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8479742.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44897..47170
                     /locus_tag="ACHAUG_000626"
     CDS             44897..47170
                     /locus_tag="ACHAUG_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8479743.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47208..48254
                     /gene="holA"
                     /locus_tag="ACHAUG_000627"
     CDS             47208..48254
                     /gene="holA"
                     /locus_tag="ACHAUG_000627"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8479744.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACHAUG_000628"
     CDS             complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACHAUG_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8479745.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48997..49266)
                     /locus_tag="ACHAUG_000629"
     CDS             complement(48997..49266)
                     /locus_tag="ACHAUG_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479746.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49528..49656)
                     /locus_tag="ACHAUG_000630"
     CDS             complement(49528..49656)
                     /locus_tag="ACHAUG_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479747.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49736..50191)
                     /locus_tag="ACHAUG_000631"
     CDS             complement(49736..50191)
                     /locus_tag="ACHAUG_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8479748.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50329..52257)
                     /locus_tag="ACHAUG_000632"
     CDS             complement(50329..52257)
                     /locus_tag="ACHAUG_000632"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8479749.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52454..53287)
                     /locus_tag="ACHAUG_000633"
     CDS             complement(52454..53287)
                     /locus_tag="ACHAUG_000633"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8479750.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53479..54183)
                     /locus_tag="ACHAUG_000634"
     CDS             complement(53479..54183)
                     /locus_tag="ACHAUG_000634"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8479751.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54206..54832)
                     /locus_tag="ACHAUG_000635"
     CDS             complement(54206..54832)
                     /locus_tag="ACHAUG_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8479752.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55158..57533
                     /locus_tag="ACHAUG_000636"
     CDS             55158..57533
                     /locus_tag="ACHAUG_000636"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8479753.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57557..58048)
                     /locus_tag="ACHAUG_000637"
     CDS             complement(57557..58048)
                     /locus_tag="ACHAUG_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8479754.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58235..59155
                     /locus_tag="ACHAUG_000638"
     CDS             58235..59155
                     /locus_tag="ACHAUG_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8479755.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59309..59653
                     /locus_tag="ACHAUG_000639"
     CDS             59309..59653
                     /locus_tag="ACHAUG_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8479756.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59843..60343)
                     /locus_tag="ACHAUG_000640"
     CDS             complement(59843..60343)
                     /locus_tag="ACHAUG_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8479757.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60871..60954
                     /locus_tag="ACHAUG_000641"
     tRNA            60871..60954
                     /locus_tag="ACHAUG_000641"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60905..60907,aa:Leu,seq:cag)
     gene            complement(61278..>61712)
                     /locus_tag="ACHAUG_000642"
     CDS             complement(61278..>61712)
                     /locus_tag="ACHAUG_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8479758.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20240 a        10663 c        13217 g        17592 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcgccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31801 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31861 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31921 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31981 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    32041 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32101 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32161 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32221 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32281 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32341 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32401 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32461 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32521 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32581 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32641 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32701 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32761 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32821 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt
    32881 tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa
    32941 gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt
    33001 ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta
    33061 gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat
    33121 ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta
    33181 ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg
    33241 cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct
    33301 gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga
    33361 aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg
    33421 ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt
    33481 tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt
    33541 cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg
    33601 ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa
    33661 taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat
    33721 taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc
    33781 aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc
    33841 tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt
    33901 cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga
    33961 tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt
    34021 atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca
    34081 tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat
    34141 ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt
    34201 cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat
    34261 gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat
    34321 tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca
    34381 aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct
    34441 gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa
    34501 acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg
    34561 taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta
    34621 tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct
    34681 gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt
    34741 tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg
    34801 ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa
    34861 aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact
    34921 gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt
    34981 ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc
    35041 aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac
    35101 agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg
    35161 tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata
    35221 aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg
    35281 ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag
    35341 aagaagatga atattccgta caccgtttca aagcggatga agcatattta gtaggacaag
    35401 gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat
    35461 gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc
    35521 aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt
    35581 ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag
    35641 gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt
    35701 atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg
    35761 atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg
    35821 gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga
    35881 ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc
    35941 gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc
    36001 aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta
    36061 cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc
    36121 aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt
    36181 tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta
    36241 aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt
    36301 taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg
    36361 atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa
    36421 aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga
    36481 aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac
    36541 atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat
    36601 ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta
    36661 ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta
    36721 tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga
    36781 agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata
    36841 cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca
    36901 agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg
    36961 ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga
    37021 gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg
    37081 ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag
    37141 cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa
    37201 aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg
    37261 gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa
    37321 aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta
    37381 aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc
    37441 atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag
    37501 caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta
    37561 gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg
    37621 aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg
    37681 aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt
    37741 attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca
    37801 aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat
    37861 ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata
    37921 tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag
    37981 aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca
    38041 gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa
    38101 aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt
    38161 tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta
    38221 cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac
    38281 aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg
    38341 aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag
    38401 ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac
    38461 aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg
    38521 ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag
    38581 cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg
    38641 gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag
    38701 actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt
    38761 cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat
    38821 gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc
    38881 gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg
    38941 ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca
    39001 agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt
    39061 ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac
    39121 ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg
    39181 tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa
    39241 cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca
    39301 agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat
    39361 caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga
    39421 acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga
    39481 ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc
    39541 gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt
    39601 aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc
    39661 tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc
    39721 gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga
    39781 agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg
    39841 atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg
    39901 aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt
    39961 ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg
    40021 aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag
    40081 ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg
    40141 ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa
    40201 aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca
    40261 aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag
    40321 ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa
    40381 gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa
    40441 cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta
    40501 ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat
    40561 ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt
    40621 ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga
    40681 gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc
    40741 gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata
    40801 ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc
    40861 agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat
    40921 catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga
    40981 tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct
    41041 tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg
    41101 acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga
    41161 aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat
    41221 ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt
    41281 acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt
    41341 ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg
    41401 atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg
    41461 acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc
    41521 aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg
    41581 gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa
    41641 gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct
    41701 ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat
    41761 catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc
    41821 accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg
    41881 ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt
    41941 aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc
    42001 gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc
    42061 aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata
    42121 tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt
    42181 aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag
    42241 ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc
    42301 ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac
    42361 tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg
    42421 gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt
    42481 gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg
    42541 aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt
    42601 tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa
    42661 ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt
    42721 aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa
    42781 ataggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat
    42841 aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag
    42901 agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc
    42961 tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg
    43021 atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca
    43081 aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt
    43141 agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca
    43201 aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct
    43261 tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat
    43321 agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct
    43381 gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct
    43441 acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag
    43501 cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac
    43561 ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt
    43621 gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc
    43681 aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag
    43741 gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc
    43801 aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt
    43861 cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac
    43921 agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc
    43981 taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt
    44041 tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg
    44101 aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat
    44161 tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata
    44221 atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca
    44281 ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact
    44341 tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat
    44401 aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg
    44461 gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca
    44521 ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc
    44581 cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca
    44641 gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc
    44701 tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa
    44761 gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa
    44821 ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat
    44881 gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt
    44941 tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg
    45001 tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta
    45061 cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt
    45121 tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa
    45181 tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc
    45241 ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat
    45301 agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt
    45361 tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat
    45421 tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt
    45481 tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt
    45541 atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt
    45601 gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta
    45661 tttgtttcga cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac
    45721 tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt
    45781 tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc
    45841 agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca
    45901 gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa
    45961 aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt
    46021 ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt
    46081 tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt
    46141 acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg
    46201 gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt
    46261 actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct
    46321 tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat
    46381 cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt
    46441 gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg
    46501 tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga
    46561 taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca
    46621 gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca
    46681 aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga
    46741 caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag
    46801 aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg
    46861 agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga
    46921 tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca
    46981 tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca
    47041 cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca
    47101 aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat
    47161 gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt
    47221 tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga
    47281 ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc
    47341 aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag
    47401 atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg
    47461 tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg
    47521 atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc
    47581 catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta
    47641 tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag
    47701 agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact
    47761 tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac
    47821 agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt
    47881 ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag
    47941 atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac
    48001 gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg
    48061 aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg
    48121 atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa
    48181 cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt
    48241 taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt
    48301 cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt
    48361 gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc
    48421 catatcaata gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc
    48481 tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt
    48541 cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag
    48601 tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt
    48661 atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct
    48721 tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac
    48781 gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa
    48841 agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag
    48901 ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca
    48961 gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt
    49021 aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt
    49081 ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc
    49141 caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc
    49201 agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt
    49261 ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat
    49321 tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata
    49381 acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca
    49441 ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc
    49501 atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa
    49561 atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc
    49621 gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat
    49681 cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt
    49741 ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg
    49801 cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga
    49861 tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac
    49921 cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac
    49981 cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat
    50041 tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa
    50101 tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta
    50161 aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca
    50221 atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat
    50281 aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga
    50341 agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc
    50401 aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg
    50461 tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag
    50521 aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag
    50581 ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc
    50641 catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg
    50701 cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt
    50761 gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg
    50821 ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt
    50881 ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc
    50941 taaaacagca attaactgct gatctttaga aagataaatc acggttttgc cttgattttc
    51001 caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc
    51061 gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat
    51121 ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat
    51181 cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa
    51241 atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc
    51301 tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt
    51361 gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga
    51421 tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc
    51481 atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa
    51541 aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt
    51601 attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt
    51661 tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc
    51721 taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg
    51781 tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt
    51841 atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg
    51901 ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt
    51961 gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta
    52021 attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt
    52081 ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat
    52141 ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa
    52201 gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt
    52261 tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac
    52321 agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta
    52381 tttataatga ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt
    52441 gttctggttt tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa
    52501 tacgatacca gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga
    52561 tagctaagac ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt
    52621 ccattttgaa tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa
    52681 acatgacagg aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa
    52741 tcaagataga tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga
    52801 cttggaataa cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac
    52861 gaggtgtttt gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca
    52921 aaacaatggc aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca
    52981 aaccaatgat gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat
    53041 cttttttttc taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa
    53101 cgacgacagc caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg
    53161 aactggcatc cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg
    53221 gcagccattc cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa
    53281 aaaacataaa ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg
    53341 agaaacccaa tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca
    53401 cgaagagaaa aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta
    53461 aaaattaaaa tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat
    53521 ataagacgta tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt
    53581 tacgctgcca tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa
    53641 aaaggcgatc gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca
    53701 gtgatgggcg atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc
    53761 cgcccaccca gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc
    53821 tgtggtaaac atttcatcat atgttttaaa attcaataca cgagggatca caccccacat
    53881 ttccatgtca tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat
    53941 aaaataagga atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat
    54001 tgcacgacca ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc
    54061 taaataatga atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac
    54121 tcgttcaatt gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt
    54181 cattttattc tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct
    54241 gttcaatcgt cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa
    54301 acagctgggt ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc
    54361 taccgatact tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc
    54421 tgacgcttcc tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag
    54481 tataagtttt catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat
    54541 catgtctacc gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg
    54601 ggctgacata aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat
    54661 cgtagtgagt atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt
    54721 gtttgattcg ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga
    54781 ccaaataaca gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc
    54841 ctccttgata gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca
    54901 aaaacaggta cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg
    54961 gatattctaa agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt
    55021 agattcatcc aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt
    55081 ttttatgttt ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag
    55141 agaaagaggt ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat
    55201 ttattttgcg acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag
    55261 gagcgagaat ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct
    55321 ttttaaaaac aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg
    55381 gtattttcaa aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat
    55441 ttgaagattt agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct
    55501 atatctatag tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga
    55561 cggcagaaga agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt
    55621 tttttaaaaa gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca
    55681 ctgtacgcaa tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc
    55741 agcgtgaact agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag
    55801 aagcacctac cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag
    55861 gggaagaaga gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag
    55921 ctgaagatgc tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga
    55981 cggcgaaaga caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat
    56041 atgccaatgg ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga
    56101 atcagatcac tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg
    56161 aactatcatt agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat
    56221 ttgatccaac tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt
    56281 ttttgattga cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac
    56341 tatcttatga aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc
    56401 atcgccgttt caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag
    56461 atcattggga ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc
    56521 aattaagcga aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac
    56581 aattgcttcc ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg
    56641 atcactacga aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta
    56701 ttgatccaag cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt
    56761 ctgctagttt ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg
    56821 cttacaaact gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc
    56881 tagagacgac ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg
    56941 cttttgctga tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag
    57001 atcttgcggt ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa
    57061 cggacatgaa tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg
    57121 aaacattgat tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag
    57181 gggaccgatt gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc
    57241 aggaactgat caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc
    57301 agcttcctgg aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg
    57361 atcaaggaat cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat
    57421 tcccacaaca ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa
    57481 tacagcgttt ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt
    57541 cagcctcgtt ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt
    57601 tacgtaataa agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct
    57661 gatcttcttg gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt
    57721 tcttcgcgtt ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac
    57781 ctagagcgtg ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca
    57841 gcttaccaaa taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa
    57901 ctagattgct gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg
    57961 acaattcttt tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata
    58021 caatgtcgaa gctagcatct tttgccatag gaaccctcct gatatacata taaatattta
    58081 ccatacatct atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc
    58141 tcatttgagt tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata
    58201 ccaaagtagg ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag
    58261 attaaagaaa atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta
    58321 ttttttgatt ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc
    58381 ctcgctttta tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt
    58441 gaatatccct gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag
    58501 ttttgtgaat gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac
    58561 tgataatatt ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt
    58621 atctttaaaa atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca
    58681 aacaactgga aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat
    58741 cgcaggcttc ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat
    58801 gactcaagta attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac
    58861 cgtcaagcca gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt
    58921 actgccgtct gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac
    58981 ccgctacgaa atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat
    59041 caatatttta ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat
    59101 gtatcgaaca actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat
    59161 caggtttatt ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt
    59221 aaacgagaat cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa
    59281 taaaaatcgt gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca
    59341 agaaatagaa ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga
    59401 attaggaatc gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa
    59461 tggagaaaca gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt
    59521 aacagaatct atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa
    59581 actagtctgg tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt
    59641 ggggatacga tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa
    59701 gccttttatc ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc
    59761 tgctatatta taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct
    59821 ttccttttgg aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat
    59881 cttcacgata aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt
    59941 tatttacaga caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg
    60001 ctaatccttg tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat
    60061 ggccagcgcc gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat
    60121 actcatgttc atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat
    60181 cgattcctaa agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat
    60241 agccataagc attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat
    60301 aagctttctt taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa
    60361 gtaaccttat tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt
    60421 ttatttattg tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata
    60481 aaaaaattat ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg
    60541 aattacttca gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat
    60601 ccaaatgaaa gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat
    60661 aaaatagtta ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa
    60721 cagatttttt caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag
    60781 atggcataag ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa
    60841 cacatgtaat cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta
    60901 gcttcaggcg ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt
    60961 gtttttttat aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta
    61021 aaagaatata ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt
    61081 gtgtgaaggt gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt
    61141 ctgatacaag aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa
    61201 aagaggtcat gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa
    61261 cgttcaaata atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc
    61321 caagaaccat aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag
    61381 tagtcaccat ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt
    61441 gctgtgtaag aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt
    61501 gctggagtag cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct
    61561 gcttgtgttt ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca
    61621 gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct
    61681 tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DAYYBH010000009        61088 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000009 DAYYBH010000000
VERSION     DAYYBH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.009"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..1423)
                     /gene="sufB"
                     /locus_tag="ACHAUG_000643"
     CDS             complement(32..1423)
                     /gene="sufB"
                     /locus_tag="ACHAUG_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8479759.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(1428..1898)
                     /gene="sufU"
                     /locus_tag="ACHAUG_000644"
     CDS             complement(1428..1898)
                     /gene="sufU"
                     /locus_tag="ACHAUG_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8479760.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(1885..3120)
                     /locus_tag="ACHAUG_000645"
     CDS             complement(1885..3120)
                     /locus_tag="ACHAUG_000645"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8479761.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="ACHAUG_000646"
     CDS             complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="ACHAUG_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8479762.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="ACHAUG_000647"
     CDS             complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="ACHAUG_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8479763.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(5386..6234)
                     /locus_tag="ACHAUG_000648"
     CDS             complement(5386..6234)
                     /locus_tag="ACHAUG_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8479764.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(6258..6944)
                     /locus_tag="ACHAUG_000649"
     CDS             complement(6258..6944)
                     /locus_tag="ACHAUG_000649"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8479765.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(6937..7971)
                     /locus_tag="ACHAUG_000650"
     CDS             complement(6937..7971)
                     /locus_tag="ACHAUG_000650"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8479766.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(8034..8290)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8366..8704)
                     /locus_tag="ACHAUG_000651"
     CDS             complement(8366..8704)
                     /locus_tag="ACHAUG_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8479767.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(8706..9062)
                     /locus_tag="ACHAUG_000652"
     CDS             complement(8706..9062)
                     /locus_tag="ACHAUG_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8479768.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(9068..10255)
                     /locus_tag="ACHAUG_000653"
     CDS             complement(9068..10255)
                     /locus_tag="ACHAUG_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8479769.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(10446..11291)
                     /locus_tag="ACHAUG_000654"
     CDS             complement(10446..11291)
                     /locus_tag="ACHAUG_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8479770.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            11525..12490
                     /locus_tag="ACHAUG_000655"
     CDS             11525..12490
                     /locus_tag="ACHAUG_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8479771.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            12867..13910
                     /locus_tag="ACHAUG_000656"
     CDS             12867..13910
                     /locus_tag="ACHAUG_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8479772.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(13972..14436)
                     /locus_tag="ACHAUG_000657"
     CDS             complement(13972..14436)
                     /locus_tag="ACHAUG_000657"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8479773.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            14682..15629
                     /locus_tag="ACHAUG_000658"
     CDS             14682..15629
                     /locus_tag="ACHAUG_000658"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8479774.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(15840..17912)
                     /locus_tag="ACHAUG_000659"
     CDS             complement(15840..17912)
                     /locus_tag="ACHAUG_000659"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8479775.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17845..18222)
                     /locus_tag="ACHAUG_000660"
     CDS             complement(17845..18222)
                     /locus_tag="ACHAUG_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8479776.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(18351..18911)
                     /locus_tag="ACHAUG_000661"
     CDS             complement(18351..18911)
                     /locus_tag="ACHAUG_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8479777.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            19061..20353
                     /locus_tag="ACHAUG_000662"
     CDS             19061..20353
                     /locus_tag="ACHAUG_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8479778.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(20413..21531)
                     /locus_tag="ACHAUG_000663"
     CDS             complement(20413..21531)
                     /locus_tag="ACHAUG_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8479779.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="ACHAUG_000664"
     CDS             complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="ACHAUG_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8479780.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="ACHAUG_000665"
     CDS             complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="ACHAUG_000665"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8479781.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            24737..25336
                     /locus_tag="ACHAUG_000666"
     CDS             24737..25336
                     /locus_tag="ACHAUG_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8479782.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="ACHAUG_000667"
     CDS             complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="ACHAUG_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8479783.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(27031..27375)
                     /locus_tag="ACHAUG_000668"
     CDS             complement(27031..27375)
                     /locus_tag="ACHAUG_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8479784.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(27512..28657)
                     /locus_tag="ACHAUG_000669"
     CDS             complement(27512..28657)
                     /locus_tag="ACHAUG_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8479785.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(28837..29811)
                     /locus_tag="ACHAUG_000670"
     CDS             complement(28837..29811)
                     /locus_tag="ACHAUG_000670"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8479786.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(29891..30766)
                     /locus_tag="ACHAUG_000671"
     CDS             complement(29891..30766)
                     /locus_tag="ACHAUG_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8479787.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            30896..32803
                     /locus_tag="ACHAUG_000672"
     CDS             30896..32803
                     /locus_tag="ACHAUG_000672"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8479788.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(33344..36622)
                     /locus_tag="ACHAUG_000673"
     CDS             complement(33344..36622)
                     /locus_tag="ACHAUG_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8479789.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(36893..37438)
                     /locus_tag="ACHAUG_000674"
     CDS             complement(36893..37438)
                     /locus_tag="ACHAUG_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8479790.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(37450..38142)
                     /locus_tag="ACHAUG_000675"
     CDS             complement(37450..38142)
                     /locus_tag="ACHAUG_000675"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8479791.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(38161..38445)
                     /gene="macP"
                     /locus_tag="ACHAUG_000676"
     CDS             complement(38161..38445)
                     /gene="macP"
                     /locus_tag="ACHAUG_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8479792.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(38458..39021)
                     /locus_tag="ACHAUG_000677"
     CDS             complement(38458..39021)
                     /locus_tag="ACHAUG_000677"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8479793.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            39249..39911
                     /locus_tag="ACHAUG_000678"
     CDS             39249..39911
                     /locus_tag="ACHAUG_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8479794.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            40035..41231
                     /locus_tag="ACHAUG_000679"
     CDS             40035..41231
                     /locus_tag="ACHAUG_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8479795.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(41332..42708)
                     /locus_tag="ACHAUG_000680"
     CDS             complement(41332..42708)
                     /locus_tag="ACHAUG_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8479796.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(42745..43611)
                     /locus_tag="ACHAUG_000681"
     CDS             complement(42745..43611)
                     /locus_tag="ACHAUG_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8479797.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(43700..44725)
                     /locus_tag="ACHAUG_000682"
     CDS             complement(43700..44725)
                     /locus_tag="ACHAUG_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8479798.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(44743..45417)
                     /locus_tag="ACHAUG_000683"
     CDS             complement(44743..45417)
                     /locus_tag="ACHAUG_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8479799.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(45601..46494)
                     /locus_tag="ACHAUG_000684"
     CDS             complement(45601..46494)
                     /locus_tag="ACHAUG_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8479800.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(46568..48265)
                     /locus_tag="ACHAUG_000685"
     CDS             complement(46568..48265)
                     /locus_tag="ACHAUG_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8479801.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(48258..49571)
                     /locus_tag="ACHAUG_000686"
     CDS             complement(48258..49571)
                     /locus_tag="ACHAUG_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8479802.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(49571..53512)
                     /locus_tag="ACHAUG_000687"
     CDS             complement(49571..53512)
                     /locus_tag="ACHAUG_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8479803.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(53621..54079)
                     /locus_tag="ACHAUG_000688"
     CDS             complement(53621..54079)
                     /locus_tag="ACHAUG_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8479804.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(54377..54895)
                     /locus_tag="ACHAUG_000689"
     CDS             complement(54377..54895)
                     /locus_tag="ACHAUG_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8479805.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(54897..55513)
                     /locus_tag="ACHAUG_000690"
                     /pseudo
     CDS             complement(54897..55513)
                     /locus_tag="ACHAUG_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(55510..56298)
                     /locus_tag="ACHAUG_000691"
     CDS             complement(55510..56298)
                     /locus_tag="ACHAUG_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8479806.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(56360..57271)
                     /locus_tag="ACHAUG_000692"
     CDS             complement(56360..57271)
                     /locus_tag="ACHAUG_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8479807.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(57312..58646)
                     /locus_tag="ACHAUG_000693"
     CDS             complement(57312..58646)
                     /locus_tag="ACHAUG_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8479808.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            58854..59684
                     /locus_tag="ACHAUG_000694"
     CDS             58854..59684
                     /locus_tag="ACHAUG_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8479809.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(59928..60177)
                     /locus_tag="ACHAUG_000695"
                     /pseudo
     CDS             complement(59928..60177)
                     /locus_tag="ACHAUG_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(60368..60562)
                     /locus_tag="ACHAUG_000696"
     CDS             complement(60368..60562)
                     /locus_tag="ACHAUG_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479810.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        17240 a        13150 c        11000 g        19698 t
ORIGIN      
        1 tatatcaagg gttctagctc ctataacagg tttacccaac actgccttcc atctcataac
       61 tgatcaatcg attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa
      121 caaagcccat gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat
      181 agtagagttg ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc
      241 tgttgtggat ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg
      301 tgtcacattc gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc
      361 gatagtttac ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat
      421 ttggtgcatt gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg
      481 cgatggagag catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca
      541 tcgttgtttt tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt
      601 ttgctcgttt ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac
      661 ggcaatacgc atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg
      721 aataagtagg tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga
      781 tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg
      841 gaacatcgac tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg
      901 aattcagcgc tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga
      961 ataattcagg gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt
     1021 cgaattcatc tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg
     1081 ctagataagc tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt
     1141 ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg
     1201 catcgaaatc gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg
     1261 tttctagaga tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca
     1321 tctcacggat cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat
     1381 cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc
     1441 ttgtggtaat gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc
     1501 caggcaagtg tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca
     1561 gcatcgccta attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct
     1621 acagttaaat gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag
     1681 gcacttgccg tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg
     1741 tcttttacat cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc
     1801 ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc
     1861 acttgacggt ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc
     1921 catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc
     1981 gcgagcagtc gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc
     2041 tctgacggct accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc
     2101 caaattgaat gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa
     2161 tccatcaatt gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg
     2221 gatattgtcc ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc
     2281 ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta
     2341 taattcgaca aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca
     2401 ttttcgttta ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa
     2461 agcaaagaaa tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc
     2521 accatctact acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg
     2581 attgaagaca cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt
     2641 aatctttttc gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt
     2701 caagacagca cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc
     2761 catgtaagag agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc
     2821 tacccaattc aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc
     2881 atggatgaac gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc
     2941 taacgtatgg acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc
     3001 ctggagtact tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc
     3061 atcattgacg tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat
     3121 ccattcaact tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt
     3181 gctgttaaga cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc
     3241 aatcctctac tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca
     3301 tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct
     3361 ttatcagaaa gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc
     3421 aagatgtggc cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc
     3481 aaaatatgcc cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt
     3541 tgtctgcccg agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa
     3601 tctgagtcaa agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca
     3661 gaagcatctc gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat
     3721 tgatctacgg ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata
     3781 ttacctgatg attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat
     3841 tcactatgtt catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca
     3901 tcttgaatga ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg
     3961 aaaataccgc tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt
     4021 actgcttttt tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac
     4081 aagtcggtaa aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc
     4141 catgaacctt gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg
     4201 cctgtttcac tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc
     4261 acccgatcga tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag
     4321 gttctcatcc aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa
     4381 tcgagccagt ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg
     4441 attcctaatt ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca
     4501 gcattccctg tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata
     4561 tagttcaata atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt
     4621 tcatttactc ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg
     4681 tctaaaattg caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt
     4741 tctccgccag aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt
     4801 aaagccattt tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca
     4861 tcacgtttcg cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca
     4921 cttggatatt gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct
     4981 aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc
     5041 atgatagcgg cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt
     5101 tctccggttt tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca
     5161 tgtagatttt ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc
     5221 actcatttta gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc
     5281 ttttatttat tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga
     5341 aataagccat tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc
     5401 ctttactggt ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg
     5461 gtgaagcact tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc
     5521 aataatattc acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt
     5581 caaccctgcg ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg
     5641 gtacgtagaa gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt
     5701 ttcttcaata tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa
     5761 gccagcatct tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat
     5821 gattgttgca ccatctttta attcggaaac atctttgact tttttagagt acaagcccat
     5881 tggttcgatg tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt
     5941 gttcaaataa ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg
     6001 gttcggtaat acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa
     6061 taatggtttc acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa
     6121 tgtcgtgtcg tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc
     6181 taatcctaca gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta
     6241 catcccccta tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct
     6301 tgaacgataa atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta
     6361 ccacgctggt agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg
     6421 gccatcgcag taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg
     6481 ggaatacttt caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca
     6541 tctgctgctt ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca
     6601 taaaatggtg ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg
     6661 cctacgattg ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac
     6721 cggaaaatat tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt
     6781 ttgcgtccaa tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc
     6841 attgagatta atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg
     6901 atttgtttaa aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat
     6961 tcctactcct tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt
     7021 catcaagaca gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac
     7081 aacgttgaca tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt
     7141 tctttcgcta aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc
     7201 taatagttct gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac
     7261 ttcttttttc ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa
     7321 ctccatcaca gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa
     7381 gacgatggtc aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt
     7441 tgtttgcggg tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc
     7501 caatgctcgt gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc
     7561 ttgtccgcgt ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt
     7621 gctctttggt acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga
     7681 ccataacagg ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc
     7741 tttgtttttt aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag
     7801 ctcaagtcca ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat
     7861 tccataaata tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc
     7921 gtttttccca ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac
     7981 atctttctct tataattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg
     8041 acgaaagctt gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct
     8101 cttccagtac ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg
     8161 tagtaagttt gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt
     8221 tacccatttt cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca
     8281 aagctttctc gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca
     8341 acttttttca agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt
     8401 tgaaacttaa gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac
     8461 cgattttatc attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg
     8521 ctttttccgc ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg
     8581 gcagattggc aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac
     8641 agtattcttt tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg
     8701 ccattttata tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg
     8761 tcctctcgta agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc
     8821 gcttttgaag ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc
     8881 ccgcttgtgt tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc
     8941 gttgaggcct tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg
     9001 tctaaccacg ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac
     9061 aattttctta cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata
     9121 tcaacccgat accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca
     9181 atgggatccc tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga
     9241 acagcagcat catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga
     9301 aacacacccg aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac
     9361 cgataaagcc gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca
     9421 catagatatt gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa
     9481 gtccttgggc cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat
     9541 caatacgtgc aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt
     9601 cagtcgtaac caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg
     9661 cgatgatgcg ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga
     9721 caagcatcgt tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga
     9781 tcaagagctt cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat
     9841 tatgtttagt tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg
     9901 gctggaaagt aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat
     9961 tctgatcata gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag
    10021 gagtcagttt ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc
    10081 caactaaata ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat
    10141 catggctaag tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta
    10201 aaatcacgcc ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc
    10261 atcccttttc ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa
    10321 gtctttccat agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat
    10381 gcagtacaaa agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca
    10441 attatctacc agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga
    10501 aggatctctt cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa
    10561 taaaaatatc gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta
    10621 gacaaaatcg agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc
    10681 acgatcatct tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa
    10741 acaccagagt ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact
    10801 agctggtctt ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata
    10861 aaaccaaaat cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt
    10921 tcgatctcaa aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa
    10981 agatagacag caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca
    11041 ttgagcaatt tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat
    11101 aataagtcgg cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg
    11161 atattcaatt cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac
    11221 agtacttctg ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat
    11281 aacttaaaca tggcaccact tccataactt tttatgataa ttactataaa aactatttga
    11341 tttaaatata caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa
    11401 ctagtaactt agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc
    11461 attttacaat gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt
    11521 atatatgaag aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt
    11581 agcttccctt ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg
    11641 cggtatctct atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca
    11701 cacgaaacaa acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc
    11761 actcatggtt atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt
    11821 catttggctg tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca
    11881 tgttatcaaa gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt
    11941 tggatttggg gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt
    12001 cttttggatt tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt
    12061 tttcgtaaga aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg
    12121 ttctctttgc ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt
    12181 agcattgttc gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt
    12241 gttcaagctt cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc
    12301 aacagctata tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt
    12361 ggatttctta aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc
    12421 tcattacgta cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa
    12481 aatcgcttaa taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga
    12541 acggccaaac aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg
    12601 caagcttttg aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata
    12661 agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag
    12721 aagctacttc tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga
    12781 cctcttgttc ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt
    12841 atttattgac gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa
    12901 aggattcagc ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct
    12961 tcagcaggct tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat
    13021 tgggtatttt attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat
    13081 ttgctgaaag caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc
    13141 aaacgattgc tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca
    13201 ctatcatcgg tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga
    13261 tgatggctgg aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca
    13321 tccatgcgaa agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg
    13381 tgatgatgtt gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa
    13441 aaagtgcact tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct
    13501 tacttgacaa ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc
    13561 tcgttgttgt tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg
    13621 aaacgccaac tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt
    13681 ttgtgatctt ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg
    13741 cacattttat cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag
    13801 actttgcaaa attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc
    13861 ttctccaaac agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg
    13921 aaaaaacagt tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg
    13981 cagatatcct tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc
    14041 catatatcct gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg
    14101 caatggattg gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc
    14161 aacggctact tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca
    14221 gacattttgc tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct
    14281 ttttcccgca aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc
    14341 tgattcgctt tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc
    14401 taaccataaa aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac
    14461 tggaacttcc tcctttttct ctatccaaaa ctacttgcca actaataaaa attgttctta
    14521 aaagtttgat aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac
    14581 ctgttctttt acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa
    14641 tgtaaaccat atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg
    14701 tcccatgttt taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca
    14761 aaggtgccca tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag
    14821 cagcaatcaa gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa
    14881 attttgggaa agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga
    14941 ccgtgatgga tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga
    15001 tcactacttc tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg
    15061 cgatacgcag tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag
    15121 aaatggtcga tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc
    15181 tagaattaac agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa
    15241 cagaatatat ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct
    15301 ggaaattatt caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga
    15361 ttgcttcatt tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta
    15421 ttttgcgggc attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct
    15481 tcctgttcat tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga
    15541 aaatcttctt agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga
    15601 agccaaatga attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa
    15661 atgaaaaagg actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc
    15721 cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg
    15781 taaattcctt cttatttctt gaagctgacc acttctatca caacctcttt catatcttat
    15841 tacttcttca ttgctgcttt tagtgctgct gctaatgggc tttcttctgg ttcttctgct
    15901 tgtgcatatt ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt cttctgctct
    15961 tttttatcaa gcaatttttc tgtcgttcca tcgtatttac aacggaaata agcaccgttt
    16021 ttaccttcga tgatttccat tttccggtga cattgcgggc atcgatgatt agagactttt
    16081 ggatcttttc ttctgcggta gttgcaatct gaattggtgc agacatagat tttgccgtca
    16141 cgtgtgtttt tctcgcgcag attggcccca cattctgggc atttcttttg ggtaatcgag
    16201 aaatcttgat attcttgttt gcttgatttg atctcattca ccaagcgttt tgtttctttt
    16261 tcgattccat tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct
    16321 ttttcccagc tttctgtcaa atctggtgtt actaaagagg gatttaccaa tactaataat
    16381 tgcttacctt taggagatac ttgcagtcct tgaggggtac gttccatcag ttcagatttg
    16441 attagtttct cgataatctc tgcacgcgta gctggggttc ccagactgta tttttccatt
    16501 tgaccaagca agcttgcttc tgacaatggt ttaggcggtg cagatagttc ttttttgatg
    16561 gcgaaatcgg gattgattct cattcctttt tcccatttga ctgtttccgt ctcagtttca
    16621 gtttcgcctt tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta
    16681 ccaaattcaa ccgtaacttt tgtttggctg actttatggg gttgagcaaa cagtcctaaa
    16741 aaacgagaga caatcatttg atagattttt cgctcttcat tacttaattt ttcataacgt
    16801 gctctgtttt cagttggcaa aagtgcatgg tgatcggtca ctttactgtc ttggaatact
    16861 tttgtctgtt ggacgatggc tccgtttttc aaatactgct tgacctcagg agcaaagtcg
    16921 ctgactgctt gcaatcgttc tttcatcgta cttttcatgt catttgtcag atatttgctg
    16981 tctgtacgcg gataagtaac gatcttatgg gtctcataca aactttgtac aagacttaat
    17041 gttttctttg cagaatagcc atagcgttga ttggcttctc tttggatctc tgttaaatca
    17101 taaggcaatg gcgcagattc cgtttttaat ttttcttgga cttctgtgac agttcctttg
    17161 cttgtactga tcaattgaac aagtttttgt gcttcttctt gtgtttttaa agcaaattga
    17221 tttttttgaa gcattttggc ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt
    17281 tgtggacgga actgctcgat tttcttttct tggtctctca ctaatgctag tgtcggtgtc
    17341 tgcacacggc ctgctgataa attatccgcg tacttcacag ttaaggctct ggtcacattc
    17401 aatcctacca gccagtccgc ttttgcccgg gcaagagcag attcatacaa tgcatcatat
    17461 tctttcgcag gtcttagttg cttgaatcca gttttgattg ctttatctgt ttgagaagag
    17521 atccataacc gcttcactgg tttattgaaa tgcacatact ctaagatcca gcgagcaaca
    17581 agttcccctt ctcttccggc atctgtcgca atgactgctt ctgagacatc ttttcgctga
    17641 gccagctgct tgattgcttt taattgatgt cctgttttag gaagtggttt gattcctagt
    17701 tttttaggga tcattggcaa cgtgtccatt tgccagctct gccattcctt attcagatct
    17761 tccggcattt tcaatcctaa taaatgacca agggcccaag taacgatcac cttgggtcct
    17821 tcataataat ttttgtgctt ttgattagca ccaagcacct tgcttaggtc tttcgcaacg
    17881 cttggttttt ccgctagaat tagttgtttc ataaaattcc tttctgcttt ctactgtgat
    17941 gggctgatga tttttcaaca acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc
    18001 ttcacaccag cgctcatctc cgtcattcca aaaggcgctg agaaagttac tttttgcatt
    18061 gatatattca taattttgat ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc
    18121 ttcgaaagac tcgaatgttt cttcttcttc aatatcttct tcccagtctt caaaaaacca
    18181 ccaaggctca ttgtccccga acatggtaat tacttgatac atactcgtgc tccttctttc
    18241 agcttttcta gactcatctt agtcactttt caagaaagtt gcaagaattt tctcttataa
    18301 aagggtcatt taatgaacat cttaaaattg ttacaaatga atctgattgt ttaaggcaaa
    18361 gtagcgataa agttattgat catccaaaaa tagctgtctt ctggatcgat tgttggttct
    18421 tttgtaaagt atcctaaaaa acgtaaggaa agataaccgt ggatcatact acgaagttcc
    18481 ctgcttcgat gaactttttc ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg
    18541 atctgattcg tttcatggat tccatctaat aaaacagctt cattggtatg cggtacactg
    18601 atcagtaact catacactgc ttgatttgaa aaagcaaagg atcgataagt ttctgcatag
    18661 atctgcagtg cttcctgacc actttttccc acaagagccc gtcgcagctg gtcattaagt
    18721 tcctgcagaa gtttcgctgt tagcgctgtt ttgacatcac gaatattttt gaaatggtta
    18781 tacagcgagg gagccttgat atccaagcgt ttggctaaat gctgaaaggt tatttgctga
    18841 acatccatct cttctgctag ttctcgaaag cagtcgatga ttttctcttg agacagattt
    18901 ctttttttca tttccatgtt tcctctcata aaataaatcc ttcactcttt cagtttacca
    18961 cgaaaaagaa agagaaaaaa gagactttca ttgataaact acatattgta gtttataata
    19021 aagagctgta gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa gaataaagga
    19081 aaaattattt aggaaaagag aaacttggga acaaaacttg tttgtcttat ggtttggaac
    19141 atttatggct ggtatcggat ttagtttagt catgcctttt atgtcacttt acatagatac
    19201 gttaggtaat tatagtacgt cacaattgaa cttttggagc ggattgactt tttcatctac
    19261 tttcttggtg actacgctta tctctccttg gtggggaaaa ttagccgacc aaaaaggaag
    19321 aaaattgatg ctgttgcgtg cttcattagg aatggcaatc gtcatttctt tgatgggttg
    19381 cgtgactagt gtgtatcaat tagtcgcatt gcgtctgctt caagggattt tttctggtta
    19441 tatcagtaat gccactgccc ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt
    19501 tcttggaacc ttagcaactg gttctgttac aggaacatta cttggaccgc ttcttggcgg
    19561 gatctctgct tcagcgtttg gctatcgccc tacctttttc attacaggga tcatcttatt
    19621 tttcgtcttc ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga
    19681 aaacatggtt tctgccaaac agattttcaa agacttgaaa tatccacacg tcgtcatcgg
    19741 tatgttcgtc acaacgatga tcatccaagc atccaacaat tccattagtc caatcatcag
    19801 tctgtacatt cgccaattgc tgcatggaca tgggaatgtt acgttgatca gcggaatcat
    19861 tgcttcgata cctgggattg ccactttgat agccgctcca agatttggtc gcttaggtga
    19921 caagattggg agtgaaagaa tacttgcgat tggtttgggt tttgcgattc tggtttatat
    19981 cccaatggca ttcgtcacga atgtttggca attagctgcc ttacgttttt tgattggtat
    20041 ctctgatgct tgtctgctcc ctgctgtcca agcattgatc acaaaatatt cgcctcatca
    20101 agctgctgga cggatcttca gttataacca atctttccaa gcaactggta acgtagtagg
    20161 gcctatgatc ggctccagtg tttccagtgc atttggttat cgcggcgtct ttctttcgac
    20221 ttctcttctt gttttggtca acttcttgct tgtacgacac aatacacaag agatccatca
    20281 gcaagaaact gcagaagaaa aagcttcagg agtctccata gcttcagggc actctccttc
    20341 tcatagacaa taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag actgtccttg
    20401 cagtgttttt gtttaggcct gttttttcaa gaactcagat aagaggaata atcccttttc
    20461 caaggtttct tttttacaac agtaacctag tcgggcatgc ttcggtaaat caaaggcact
    20521 tcctggtact aatagtactc ctgtttcttc taataaattg atacaaaatg tctggtcatc
    20581 ttcttcgatg gctaatttaa tgaaagaagt agagacgtaa ttgggagcga caagttcaac
    20641 tttcggttca ttggctaccc attgttttaa aatcacaagg ttctctgtaa tgatcttttt
    20701 gtttcgcgcc aatatttttt ctttattctt caatgcatgt acagcaagat catctgacag
    20761 gactccgccg caaatcatcg tgtagtctcg atactttcga aaaacttctg ccaactcagg
    20821 tccagtagct gtccagccaa tacgaatacc tggaatggag taggttttgg atagggaatt
    20881 ggttgctata cctttttcat agacatctac gatcgacaag aattcacctt tatccgttaa
    20941 aggcgcatat acttcatcta tcaatacata tgcatctact gtacgagcaa tttctgcgat
    21001 tttttctaaa gttttccgat ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca
    21061 gatgattttt gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa actgccaatc
    21121 ttcttcttcc ttcaaatgaa caaagtcaac agcggcacct aaagatttgg ggatgtcata
    21181 taattgctga taagtaggaa gcatcgaaac aacatggtct cctgcttcca ctaacgcgta
    21241 tagagctaaa agattagcac cagtcgctcc attcgtttgt aagatgtttt ccggatcgac
    21301 ggtttgataa agagaagcga cttcttgttt gaaaacatct gatccttcga tccagccata
    21361 gtccaatggt tcgtttttgc gtttttcgaa aaatttactt acgctagtcc catctaagcc
    21421 aacgagttcg tctaatgtca atgcttcgat cgagctttgt gcaatatcat ataccgcttt
    21481 cttttcccat ttatttagcc attcttctac accgaaattt gcgattttca tgtttctttc
    21541 ccccttatat cttgatccag cctacagcaa gcaacccgat tgaaagcagt gcggccacac
    21601 aaatcagccc aatcattact ttttcttttc ccgtaatttg atgtttggtt tcacgacaag
    21661 cgatccagta gaagcaaaaa ccaggaagat aggcaatact acataaaagg acataagaaa
    21721 atcctgctaa aatgatgccc atcaattcaa aaactacagc aatcccaccg atcactactt
    21781 gttttttgat tccttctttt aaagaaaact ggacttgata gatcccgaca agtagataac
    21841 aaatcaaaat tgccgctgta cataaagaat aggcaaattg ataggcataa tcagtaacta
    21901 agaaagtcaa taagaataga ttagtcaaga ttgcagtaac aactaacgaa taagttgggg
    21961 catttttttt attcgttttt ccccaacctt tcggcaacag accgtctcga gccatcaaca
    22021 tcgttgtttc tgctgggagc atcgtccaag acaaccaaga accaatgatc gaaataatca
    22081 agccgatatt gatcagcgct gctccccaat ttcccacgat tccttttaaa atatttgcca
    22141 tagcaggttg tgaaatagtt gctaactctt cttgagataa gacgccgtat ggcaagatcg
    22201 aagcaaggat atagataaac aataaacaaa gcagaccaat gatcgatgct tgctgggctt
    22261 ctgaacgttt cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc
    22321 acatcataac catcaaacaa cctttaatct gcaaccagat cgttccttgc tgccctgttc
    22381 cattcactag attatcagat acgttacccc aaaaatcagc agtgaatata ttcactttaa
    22441 atgcgataaa tacaaatatt aaaaatagga ataatggcac taatttacag atcgtcacga
    22501 ttgtattgat gacgctcgct ccttcaactc cattgtttac taataaagtc agaccccaaa
    22561 taaaaatact tgcaaataaa atagaaggaa cattttgtcc acctttgaat atcgggaaaa
    22621 attctcccat ggcgctcatc agcatcgttg caaaagcaac atttcccagc cacgcagaca
    22681 accagtaacc ccaaccactg atgaagccac ctaatttacc aaatgaagct tccgcgtaac
    22741 cgaaaatacc accatctaga tctggacgct tttctcctaa atgattcagc gataatacca
    22801 aagctaaaat accaatccca acgattatcc atgaaaggat cgctggccct ggagctgcag
    22861 aactagccag atcacttgtg atcccaaaca caccgcttcc gattgacgaa ctgacaacca
    22921 gagcaaccag tccccatttg ctaatacccg tcgatttctt ttccatttcc atcaccctca
    22981 cttataaaat tgaacaaatc acttgcaaaa gattcccttc tattttgcaa ctgctcttgc
    23041 ttaacaaaaa gcaactttat tcatattatt ggatattatg aactttttac acaagtattt
    23101 caagtataaa atgaataaaa attaatttta ttgcaaaaaa acagctatgc tatccaaaag
    23161 aaaaggtgtt tcttttgtta gccatagctg ttttgattca tttaatatcg cttaaacgta
    23221 ttgtaaaact tttgtttctt gatatgcgtg gtcgatcaaa ccaccaccca gacattccat
    23281 tccatcataa aatacaactg cttgtccagg agtgattgca cgtacaggtt cgtcgaatac
    23341 gacttctgca cgattatcat ccaataagcg aactgtcaca ggaacgtctt gctgacgata
    23401 acggaatttc gctgtacact tgaattcttt tggcattggt tcattcgtcg taaagtggat
    23461 ttcacttgca tccaagcttg tggcatacaa tgcaggatga tggaaccctt gccccacata
    23521 taatgtgttt gtcgctagat cttttcctac aacaaaccag ggttcttgcg aatctcctcc
    23581 gccaccgatc cctagtcctt gacgttgacc aatcgtataa tacatcaagc cagcatgttg
    23641 tcctttgact tcaccatcaa gagtgaccat atttcctttt tttgctggca gatagttgct
    23701 caaaaattgt ttgaagttct tttcaccaat aaagcagatt cccgttgaat cttttttctt
    23761 agcagtcgca aggcctgcgc gttcagcaat tgcccgtact tctgattttt ccatgccgcc
    23821 tagagggaac atcgtttttg aaagctgttc ttgtgataat tggctaagga agtaagtttg
    23881 atctttatta ttgtctactc cacgaagcat gtggaccacg ccgttttcat cgcgagtgac
    23941 ttgcgcataa tgtccagtag ctacataatc ggcgccaaga tccattgcat aatccaagaa
    24001 tgctttgaat ttgatttctt tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg
    24061 gtattcggct aggaaatatt caaatacacg atcccaatat tctttttcaa aattcacaga
    24121 gtaataagga atcccgattt gagcagctac tttcgctaca tccttataat cttccgtggc
    24181 cgtgcataca ccgttttcgt ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc
    24241 gtatccttgt tcctttaaca aaagtgctgt tacagatgag tcgacaccgc cgctcatccc
    24301 tacgacaacg cgtgttttgc tgttgtctgt catgatatca ccatcatttc aattagattc
    24361 tgaaacatct acgagttgaa ggtcgcaact tttcagtatt ttacattata cgtcatattt
    24421 ctgaaaattt catcccttaa ttatcaagtc gtaaaaatac tgttctactg gacctattca
    24481 gtacacaaac atgatagtca accaaaaaat cactcatctt ttgagcattc gatataaaaa
    24541 attggcatac tcataaaaag ggccagcctt tcggcaataa ggaataattt tataaattgt
    24601 gaaaaactat ttttcaaaat tgcttcttat cgcttaaagc tgcacgtctc atttacaacc
    24661 ttttcttttt agatgttatg agttgctcag ataagtgatt ttgtttactc taagaaaaaa
    24721 gggaggcaaa agatacatga attactttat cagtgatatg cactttttcc atgaacgctt
    24781 attaggaaaa aacgactttg ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca
    24841 gttgatcact agctggaatg cagtaataaa agaaaccgac catgtttatc atctaggcga
    24901 ccttgctatg catccgcaat atgaaaaagc aaatgcagaa attttggaac tggtcactca
    24961 actgcaagga acaatccact tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta
    25021 tttagaaaaa aatgatccta ggcttactga cggaagtccc aaattttatt tttatgatgt
    25081 aggtgccatc gttaaattcg accatcaaca atattacttg actcattatc cgatgttgct
    25141 cgggcctaac gacactatcc gcaatcttca tggacatatc catcattaca gcgtacctat
    25201 cgagaacgat gtcaatgtag gcgtagacgc accggaaaga gaactcttaa aggaaaaact
    25261 accatttggc actccgttat ctgagcaaca aatcaaagaa atctatgaag caaagaggaa
    25321 gtacgatagg tcgtgaaaaa actgccctat atcaagtatt aaacaaataa agataattac
    25381 tcatattttt catttgagta ggcttattgc cacaggaaca ttaggatcag aaatggagtt
    25441 gtaacaagac ttctgtcaca actccattcc atactattcg tctttttgct catggtgttc
    25501 ttttaccagt tttttctgta attctttatc ataatttcgg gcagctgacg ttaaaaataa
    25561 aacgattccg cataataaaa tcccgattcc ggaaaacaag aacatttttt ctacccctaa
    25621 acgatcagct aatggtcctg caaataataa tccaattgga cctgccaggt tcatcaaaga
    25681 gttgaagttc cctaagacac gacctaaaac attcgattca taactctgct ggatcattgc
    25741 catcagcagt gtattgaaat atggcgttgc acaacctgct cctgcattca agataagaaa
    25801 gtacaagaac ccttgactat cgtttggaac caatccgctt gcaccgatcg tcaggcccat
    25861 aatgatgtag gctataataa taggtttcat tcggtctttc catgttccta taaaactgat
    25921 cagtgcaccg cctaagagca ttccgactgc gtagactact tccactgctc ccgcctgtcc
    25981 aacggaacca ccaaagtaat ctagcgtcat caatggatac agactgattg ctggcatgaa
    26041 caacagcatc acaaatgcac cattgatcgt tatgtaccat aaccctttat tatccatcaa
    26101 ttgctgcagc ccaaattttg catctttaaa aatagtaagc ggaacttcca atatttcagg
    26161 agaaactttt gggatcttca caaaaatcaa taagccgaca ccaaaaactg cccccaatac
    26221 atctaataaa ataaggtaat tgacaggaac gactgaaaac aaagccgcac ctagagctgg
    26281 cgcaatgatg aaattcgctg attggaccat tcctaactgt ccattcgtcc ttgttatgtg
    26341 agaagatgga acgattgtcg gcataatcga ctggatcgtt ggcatttgga atgtttgtgc
    26401 gaccgaacgc ataaacaaag acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt
    26461 tccgactact gctaaaataa gcgcgaaaat cgctacaata atatccgtga caatcaaaag
    26521 agctttttta tcccagcgat ccactagcgg tcctacaaat gggcttagta aaaccatggg
    26581 aatcattcct agtaaagtag caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa
    26641 ataccagatg atcgcatatt ggacgaccat actagtgatt cctgacaaaa actgtcccga
    26701 taaaaagaac aaataatttc tcttccaatt cgttaaaacg accgactgtt cattttccat
    26761 aaacatccct cttttctttt tcattttgta tagcgtttgc acaacaatca ttatacggga
    26821 ttcttttttc aaaaaatacc tttttcccta aataacaatc gtttcaattt aaatttcttt
    26881 acttagaaac aataaatcag caaaatatta aaaataacaa catgtgttaa atttaaaaat
    26941 aaaaaagaca actaatttgc gtcgtcctta caaagttagt catctaacaa taggccgttt
    27001 caaaactagt tgtcttttta tatccacaga tcattcgact ttttcgaata acccacggct
    27061 aatcatccca tccatcagaa aataaaattt atcttggctc attttctgtt ttggatcttt
    27121 gaaaatattg actgctttca atccatttgc tgtagtgatc gacattttca atgtttgaat
    27181 atcttttgat acagtgattt ttaatttgaa tccttctttg ctttgcggcg tggcatctaa
    27241 tggtcggatc aattgataat tgcctccgtt tgtttctgta aatccattat cgcgcaatgt
    27301 atatcttttt gcatcaggat gcaaacgata gctaattggt gcgcccagta ccgttgctgt
    27361 tgtttcaaat gccatgattt tcacctcgat aaaattagtt tctctttatt ataaaagttt
    27421 atcctgattt ttactaggtt tttcagttaa gttccctaaa aaatgaaggc gttctctcag
    27481 acatctgttc atataagaat gacttcacta gttatttttt gacagcacga actaattgat
    27541 caaggaaata ctggatgtcg tcttcggtat ttcccaaacc aaagctgata cggatggatt
    27601 cttttagtat cggtgtatct tctccataca ttgcttctaa tacgtgtgaa ggctcgatat
    27661 ttcctgctgt acaagctgaa ccagtagaaa tagcgacccc attaagatca agcttcatca
    27721 gtagcaaatc atttgcttcg ccaattaagc gaagattcaa cacatgaggg agtttattcg
    27781 ttaaatcacc atttacttga taagggattt cgtttgcttc taatgtatca agaatcaatt
    27841 gctggaatcc tttatatttg ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa
    27901 ctgcttttgc cataccccag atccctgcta aattttctgt acctgcacgc cgcttttctt
    27961 cctgctcccc gcctaaaagc aatgcaggga tatttactcc gtctcgttta tataagaaac
    28021 cgacgccttt tggcccattg attttatgag cagagatact taataaatcg attcctaatt
    28081 cttctggatg gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg aaataagctg
    28141 gatgatccgc taaaagttca ccgatttctt tgattggcat caaatttccg atttcattgt
    28201 tcccgtacat cacagagacc aagatcgtat cttctcgtaa cgcttttttg acctcttcga
    28261 tcgacagatt ccctttttca tctacaggca gatatgtaac gtcaaacccc tttgtctcta
    28321 aatatttcat ggtattaaga acagcaggat gttcgatagc tgtagtgatt agatgtctgc
    28381 cttctttttg acgcgagaac gctgtttcta agatagctgt attatcccct tctgttcccc
    28441 cactgttaaa gatgatctcg tgaggttttg cttgcagact gtctgcaatg atttgtcttg
    28501 ctgtttccaa tttttcatgc gcgtggcgac caaatccatg tatgcttgaa ggatttccaa
    28561 acgtgtcttg catgattgcc gtcatcgctt caattacttg tggatgcatg ggggtagttg
    28621 ccgcatgatc cagataaata gttttcactt cattcatccc ttccaaatta ttgctaggca
    28681 cattctagca taaaatagcg aacaaaccaa agaagagctc atttttgtct agaggacgtt
    28741 tcatgggcat agaaaaaagg agctgtgaca aaagtcttgt cgcagctcct tgaagctgct
    28801 cacttctgtc acaacttctt tccctctttt ttttgtttat tcgaattctt tttcttccag
    28861 acggaataac ggactcatcg gtgtattttc atggatgcgt ttgaccgctt cacccattaa
    28921 ttctgaacat gtaacgattg ttaagttttc tggatgacga ttttcttctg tgaaaacaga
    28981 atcagtgata caaatatctt tgattggtgc attgtctaaa atacttttcg catgatcgga
    29041 caataatcca tgtgacgcac aggcatatac ttcttttgcg ccgttttctt ttaatacatt
    29101 tgctgctcgt gcaagcgttg ctcctgtgtt caagatatca tctaccatga tacatgtctt
    29161 tccttgaaca tcaccgatca cataaccacc ctcagttgaa gcatcatctg catgatctac
    29221 gatggctaat gtgctgttca aatattctga aagactacgt gcacgctgaa caccgctgtt
    29281 ttttggcgaa acaactacga tgtcatctcc cgtcagacct aattgacggt aataatgagc
    29341 aaacaacggc atcgtaaata aattatctac aggaatatca aagaatcctt gtacttgaac
    29401 cgtgtgcaga tctaaagtca gtactcgagt tgcccctgct tcttcgatca agttagcgat
    29461 caatttcgca gtgatcggtt catgaggttt cgccgtgcgg tcttgcctag catacccata
    29521 gtaaggcaat acgacattga tcgttttcgc acttgctcgc ttcattgcat cgatcatgat
    29581 cagtagttcc atatagtaat cattgacagg tgcattcgtt gattgaacga tatacacatg
    29641 atcccctcga atactttctt cgatattgat ggaaatctcg ccatcactaa attgcttcac
    29701 gacgctttta cctaattctg ttccgaacac tttcgcgatt ttttcagcta atggacggtt
    29761 gccattcaaa ctaaaaatct tcaacgtgtc atcctgatac ttattactca tgtagtcctc
    29821 cccaaaactt ctctattctt cgatagttta acatattttt ccaagatttt ctgttaattt
    29881 cagcaaattc ttaaggaagt gaaactgttc cgttactcca actggcagga atgactttat
    29941 gataatcgcc ccagttgtcg tgtactttgt aataacgttc tcctgtttca gtttcaaaat
    30001 aagcgtacgc tgtcacccaa tgattgccat atgtactgcc aagtactttc aaaataccaa
    30061 tcatcacagg tttgccttca cgtatacgtt ttgtagctcg ctgccatgaa cctagaagag
    30121 ttgctcgtgc acgctcatga ttgccattct tctttaaaaa actagaaatc cctgtactaa
    30181 cctgaaaagg aatcgtagga agtcctagtg gttggatctg gctacgcaat gccttcgtca
    30241 aaaccaacga atcagccgta tttttcttgc gaatctcatt tggaagcatc caccccttac
    30301 gatagtcttg ataataggca aggagaactg ctgccgcgta agtaccacaa agatagcctg
    30361 agggggtagc ccatttctta tacctcaaaa aacgttcttc tggcaatccc acccagcaat
    30421 ttgcgagttc ctttcctgac cttttttctt cgatcaaaac gggaatatct tcttttctgt
    30481 tgatccatct agataactcg attggctctt cattgatcag cttccatttt ttacaagaaa
    30541 gaccctcttg ataagtatat tttgctggag aatattctac cggatgtcct gattgatcga
    30601 taatcacata actttgatct tcaaaaacca gagctaaaaa aagcaattta ctttttcttg
    30661 actgcagctg ctgagtgctg gctaattttt taattttttt atctactttg aaatataaag
    30721 gtttatccac tacttgattc agttgggatc gcctcctttt tttcattata agtgaaggat
    30781 tgaaaaaaac aaatcagttt gattgttcga attttcaatt ttttatgttc tatttgttaa
    30841 ttccttcatt ttgtaagata atgggcacac gaaacaaaaa aggagcgatc gatgaatgaa
    30901 caaagaacga ttgaaacaag atttctatga agcagtcaat gaagaatggc aaaaaacagc
    30961 aaaaatccct gcagataaac cagctacagg cggtttccaa gatttagttg acgggatcga
    31021 ccaacttttg atgggagaaa cagacgatct gcttgcccat ccagaaaaag tgtcttcaga
    31081 aaaaatgaaa catttccttg catattatca actggcaaac gattatgacc gaagaaatga
    31141 gttgcatgct caaccgcttc aacctataat cgaaaaagtt gaatcattgc agtctttcga
    31201 cgaattgaat aagcaatttc cagaatggct gttggataca ttaccattgc catttaatct
    31261 ggatgtagat gcagatatga agaatgcgca aacgaacgcc ttctttgctt atccgccttc
    31321 tttattcctt ccagataaga cgtattatgc atcagaccat cccaacggtg cacaactaat
    31381 aggcgttttc tttgatatgg ctgttcagtt attagagctt gccggcaaag aaaaaagtca
    31441 ggcagaagca atcacggaac aagcagtgca atttgataaa ttgattgctc ctcatgtaaa
    31501 aagtgcagaa gaaaacgcag attacagcaa aatgtataat ccgcaatctt ttgatacatt
    31561 tattaaatac acagataaac tggacttgaa aaaacttgtc acgggtttga ttgggacaac
    31621 tccagataaa gtgatcgtaa ctgatccagt gtacttcgat catttgaatg acatcctgac
    31681 agaagctcat tttccgttat tcaaaagctg gatgatcatt aaaacagtac gttctcttag
    31741 tggttatcta tcagaagaat tccgccaagt cagcggaatc tattctcgcg tattgtctgg
    31801 aacagacgaa gcaatgcctc agaaaaaagc agcgtattac cttgcctctg gacaattcga
    31861 tcaagtagtt ggcgattact acggaaaaaa atattttggc gaagctgcta aaaaagacgt
    31921 agaacaaatg gtgaaaaaaa tgatcgccgt ttataaaaaa cgtctagaag aaaatgactg
    31981 gcttggggat gcaacaaaag aaaaagccgt cataaaatta aataacttag gtatccaagt
    32041 aggctacccc gataagatcc cagcattatt cgaccagttc aaaacagtac ctgcagatca
    32101 gggtggtact ttgctttcaa atactcttgt tttcagcaaa ctcacattaa aagatcgttt
    32161 cagcaaatgg aacaaaccag tggatcgttc agaatgggaa atgagtgctg acacggtcaa
    32221 tgcttattat catccatttc ggaatgtgat cgtctttccg gcagcgatcc ttcaagcacc
    32281 attttacagt ctagaacaat caagctctgc taattatggt ggaatcggag ctgttatcgc
    32341 tcatgagatt tcccatgcat ttgataataa cggcgcttta tttgacgaat atggcaactt
    32401 gaacaactgg tggacagaag aagatcttac acaatttcaa aacaaagcac aagcaatgat
    32461 cgaagaattc gatggtctgc cttttgctga cggaaaagta aacggaaaat taaccgtttc
    32521 ggaaaatatt gctgatgcag gcggtctaag ctgtgcattg gaagcagcaa aaacggaatc
    32581 agatctttct atcgaggaat tctttgtcaa ttgggcaacg atttggcgta caaaagcaaa
    32641 aaaagaatac caacaacttt tactgcaaat cgatgtccat gcccctgcta aactccgagc
    32701 aaacgtgcaa ccaaaaaacc ttgacgaatt ctatgaaaca tttggtatca cggaacaaga
    32761 cgctatgtat ctagcgccag aaaaaagagt gcgaatctgg taagaggttg tgccagaagt
    32821 ggtcagcttc aagaaataag aaggaatttg ggaaaatcgc ttctcaaatt tttgtgacat
    32881 ttcggcttat ttttcgaaga agttacttct ggaataccat gtattagagg ttgtgaaaaa
    32941 gctgcttagt ttcaagtagt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
    33001 aaatttgact tactaccgag aaactggctt ttgaacaccg tttatcgaga ggttgtgaaa
    33061 tcagcgtcct gacctgagta ttagagaaaa aaacggaaaa atagttcctc atattttgcc
    33121 gtttgttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gagattgtga
    33181 aaaagctgtc ctgcatcaag taataagaac agccaatcaa aagcagcaag gagaacctca
    33241 tctataaaaa gatagaagga agtgagacat ctattggtct catttccttc tatttttact
    33301 ttcctcagtc aattgtttta catcataaaa ggagaaattc cacttatttt tttcgctgtt
    33361 tcatgacata aacaactaca ccgattaaca aaattcctcc agctaatgct agcaacgggt
    33421 tattaaattc tcccgttttt gggagtcctt gagtgccatt tttttcactt tcttttgctg
    33481 tcctatccgg atgagaaggt tttccatttg gttttccatt tggtgattca tttcctggat
    33541 tttctctttc tggtttgtca tttcccgggg gcacatcggc agtttctcgc acagtgatcg
    33601 ctttttgctg ggtaacagat tctgtatgag cagaccattt ttccccatca aatatttccg
    33661 aacgataagt gacttctagc gtatattctc cttttgaagg tgctgaaaaa atagcggtat
    33721 gattagaaaa ttgagcactt gactgtgttt caacgatttg gatcttttct gggaaataac
    33781 gcatatcttc aaagacaggc gttccttgat ttggtgtgaa agttgacaca aaaactgtaa
    33841 aaggcttcag ttcaatgata tttccttctg cattctccgc cacaccattt gcttcttgta
    33901 taataatcgg attattttgt tcatttggtt ttgtctgatt tggttcttgt acgattgcat
    33961 actctggata ttccgaagac ggtgaggttt ctccgtcaaa aatagcacct acagataatg
    34021 gaagaccatc cactctttcc ttttctgttt gacgattccc cggtttaatc tgcatcagtc
    34081 catgaacatg gatacttcca ttttcatagc gcgtaacagg ctgctgacct tctttcgaac
    34141 atgtatttag agactcgatc caatcttttg atatggtatg tccaagagta ttttctgatt
    34201 gtttctctcg ttcattccaa tagtagttca acgagtggta gatttgatct tcgttttgat
    34261 tcaatgggcg gatctgctct ctcatctcaa gattctctgt tcggaaagat aagataccag
    34321 aagcatggta gtctgtgttt cctgctgaag aggtataaag agccacatta tatgaaccgt
    34381 tattaaaact tgtactgcta ttgacgataa tatctgttcc gctattggaa tcgattcctt
    34441 tcgcaagatt ttcgaaagac aaactattga tcaattcatg agggcccgtt aaagaagaac
    34501 cgcccatttt gaatccatta ccgttacctt ctccctcaat tcctggtacc cagccgtttc
    34561 gataagcaac tgagtttttg atgattactt ttccgatcga acctgtttca tttttagcaa
    34621 aaaggtccca accgtcatct gcattatgat acgcgataca accgtcaaat acatttcctt
    34681 ctcctattgt cagtttggcc gcaaatccat cggcatcttc aaatcctgga tcggcatttt
    34741 caaaagatgt acagttcttg actaagttat gcgcgggcca tgcttcgaaa ggatcatttg
    34801 agctaccgct gatttggatt cctgtatttc cattccgata agcatgaatc tcttcgatca
    34861 aattatgatt tccagaaatc tgtaatcctt tttgcatagc tgcactattt gtaatgtcaa
    34921 accctcgtaa accccagtag tgactgaaca gtgtcatccc atttccagtt cctttaaaat
    34981 caagtacagg acgcttgttt tctggatttt tctctgcaat caactgaatc gggttttctc
    35041 ttgtcccgtt aacgcctttt ggaatcgtca atcctctttc gaaggaataa gttccaggcg
    35101 ccatgacgat tgtctgccca gcatatgcgt atctcaatgc ttgtgtcaga gataatggat
    35161 tttgtctgct tccgtttcct gcatctgacc cttctggtgt tacataaact gtcgaatatt
    35221 taggtttacg gtagtccact gtatgaagaa tctcttttgt ttcataacta gtcatctcta
    35281 cgtattcttc tggcggatag tttttatctg gcgtaaaact gaaagtaaat tcgttcgttc
    35341 cttccatcag ttttgtctga taatcaaatt cccgattcgc agtgatagcc aagttttttg
    35401 cgttcttaag cacctctcct gttgctttat ttttaattgt caataatccg tcagcatttg
    35461 ctctaaagga catagtatga tcttggtttc ctgaatgatt actagaggtc acagtgtaaa
    35521 tcggatcaat caagcgagta ggtctttctt gcccaggagg atctgtcgca ggatctgagt
    35581 aagcaacttc gattccttct accttgatcg tcatattcct tgctgcagca aatcctgcat
    35641 aaataaccga ttcatctgaa gcgtataatt tttcccagtc atacatgatg tcagaagcca
    35701 tttcttctcc tgtttcagga tcgtaataat atgcctctaa accagtattc gtttttttca
    35761 tctctacgtc cagtttagta atcgagttcg tggccggaaa cagattccca tgaccgtttc
    35821 cgaatatatt ataagaacct gtttcttttt ccaagaaccc tgctgaagtc tctaaaggtg
    35881 ttgtttccac tgagacactc ccaggagctg gcgcagcttg cggatcttca gatgatattc
    35941 ctgtgattgc ccgtgtcccc aatccaagtc gcatcgtata tttgctgccg ctcgtgttga
    36001 ccacttcttg tctatccgag tcccagacat attcgatccg actgcctagt atggcaaatg
    36061 aatttgaata aaatgcctgc ccaaacaaat gattttttgg tactgcgtct cttaccatta
    36121 aagcaaatcc ttcttgggca ttcgtatagg tccaagattc tatttcgatc gtcgcccgca
    36181 tcctaaagtt gatgtctttg gggattgctt gataataata ggtcattcca tctgatccgc
    36241 ttgactggaa ttttccgccg ttattcgtgg aagagagccg cacgccgtct tctaatggtt
    36301 caatggcata cctactagca cttgtgcttt ctccgaaaac aacccctttc cactcttcat
    36361 ttgcttcttc agattgcttt tttgtctttt cattttctga ttctttcatc ataggatcat
    36421 ttccttcttc cgtatccata tcttcactag ttcttggtat ttcctccatt gttgacatcc
    36481 cttcaatgtt cacgtgtgca ttttcttcgt tcacagactg ttgttcgtta gcagcaactt
    36541 caacgacaga aattaaaacc ggggtactga gcataaggag attcatcatt agcgcactgt
    36601 attttatacg tttttttaac aagtgtattc ctcccgtaac aaattagaaa gcgtttactt
    36661 aaagtattac ctaatttatt tttactgtca ataaaaaaca aaattataat aagtgtattt
    36721 tataaaaaga aaaaatataa cttaagttgt tgataataaa gagatttatt aacggaaaaa
    36781 gaataagttt ttttaaaata acgtataaaa aagagcataa aacaaagtcg tttgttttat
    36841 gctcttcttt tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa
    36901 tttctgcgcc tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga
    36961 aacttgccac ggaatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg
    37021 ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc
    37081 gttctcgcaa acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta
    37141 tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca
    37201 attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa
    37261 aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa
    37321 acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca
    37381 gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct
    37441 tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc
    37501 tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat
    37561 tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc
    37621 ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg
    37681 cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc
    37741 ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat
    37801 tcctggtaat tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac
    37861 tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc
    37921 tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc
    37981 taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga
    38041 aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat
    38101 ttttacttct tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt
    38161 ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga
    38221 tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt
    38281 gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg
    38341 ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg
    38401 tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta
    38461 ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat
    38521 caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac
    38581 ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat
    38641 atgaaggact tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga
    38701 cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg
    38761 tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt
    38821 tcgaaattgt ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc
    38881 gccaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc
    38941 tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc
    39001 aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt
    39061 ttcatttttc aatttttctc aaaaactcct tattagttta ccatagataa accttagatt
    39121 ttctctaaat atagaaaatc cttctttagt ccgatactct ttctagtagt ccgtatgcta
    39181 aaataagtat gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag
    39241 ggtttattat gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct
    39301 ttgtccgaaa caatagtttt tcttttgttc taggactttt attgataggt gtagttttag
    39361 ttttgtgggg actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac
    39421 tgactgacga acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat
    39481 tttttcgaca aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc
    39541 agcagacagc caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag
    39601 ctttattcaa agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt
    39661 atacgcatct gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg
    39721 agatcaaaaa caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc
    39781 tgtcagcttt aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg
    39841 acgtagagat atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac
    39901 aaaaggatta actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct
    39961 gtttcgagta ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca
    40021 aggaggaatt ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg
    40081 atacacttga agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt
    40141 cacaacaagc ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca
    40201 aactaccggc ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg
    40261 atgcacagtc tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac
    40321 aatcgatgtt gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg
    40381 aactgatgta tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca
    40441 tcttccagcg tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc
    40501 aaaagattgg tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc
    40561 tgctaaaaga taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg
    40621 cactgaatat ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca
    40681 ttccagaagc gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca
    40741 atgattacac gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta
    40801 gacagatcac gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct
    40861 tggctgaaaa gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg
    40921 tgatcgcatt gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg
    40981 aaacaacaaa cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa
    41041 cagcgaaaga aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg
    41101 atttgatcga aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc
    41161 gtcatgcaga agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt
    41221 taacccaata gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac
    41281 aactgttgat gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc
    41341 attcatcttg ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac
    41401 gataccgaac acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa
    41461 gatggagacg attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt
    41521 gccaaacatt tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc
    41581 catgatattc gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc
    41641 tgccacacca gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt
    41701 cataaactta ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata
    41761 catcatcgag atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc
    41821 cactgttcca tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc
    41881 agtatacgga acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat
    41941 cagtatcgaa ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc
    42001 tgccattgca acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag
    42061 cacaccggaa aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat
    42121 tcctacaatg ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa
    42181 acgtaatgct ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac
    42241 cgcaatcaag tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg
    42301 aagttcggcc cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac
    42361 agaggaaacc gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa
    42421 ttcaccaaat acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc
    42481 aaattctgcg aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag
    42541 tgacagtgct ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt
    42601 accgatgatc gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat
    42661 ctcactcgta tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc
    42721 ccattttagg ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc
    42781 taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc
    42841 gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa
    42901 gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca
    42961 taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc
    43021 aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc
    43081 caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa
    43141 tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc
    43201 atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta
    43261 tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa
    43321 aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc
    43381 atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa
    43441 aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc
    43501 accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa
    43561 accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct
    43621 ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt
    43681 tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata
    43741 aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt
    43801 cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac
    43861 catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga
    43921 tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga
    43981 tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg aagataaaag
    44041 cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat
    44101 ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc
    44161 catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt
    44221 gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt
    44281 caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt
    44341 catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca
    44401 ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt
    44461 ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt
    44521 ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc
    44581 cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc
    44641 acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat
    44701 ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc
    44761 ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa
    44821 ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc
    44881 tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag
    44941 acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc
    45001 aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta
    45061 agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt
    45121 cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct
    45181 ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc
    45241 atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc
    45301 tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc
    45361 ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa
    45421 aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt
    45481 ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta
    45541 atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta
    45601 ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc
    45661 gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat aaagatggta
    45721 tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt
    45781 cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc
    45841 ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac
    45901 gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat
    45961 ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga
    46021 atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc
    46081 taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata
    46141 gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac
    46201 cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag
    46261 cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg
    46321 ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc
    46381 tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa
    46441 ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt tcattttctt
    46501 caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt
    46561 ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca
    46621 tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg
    46681 acccgcgttt catattcggt actctttctg gccctggatt ttctactcct acgatctgaa
    46741 tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg
    46801 gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc
    46861 cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg
    46921 cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa
    46981 atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt
    47041 cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg
    47101 gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt
    47161 tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta
    47221 aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt
    47281 cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc
    47341 cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac
    47401 caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga
    47461 cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt
    47521 ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc
    47581 taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat
    47641 acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca
    47701 ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat
    47761 gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt
    47821 ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa
    47881 tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa
    47941 tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca
    48001 aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt
    48061 caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac
    48121 cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga
    48181 ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc
    48241 ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca
    48301 gcacctaaca ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc
    48361 gggaatacgg accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt
    48421 ttgtccgttg tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca
    48481 gagactggaa ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat
    48541 tgtggttggc cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt
    48601 gcttcatcca tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa
    48661 ggcaagaaac gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca
    48721 tttttgtcat tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta
    48781 gcaggagcca tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca
    48841 gcaccttcta atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag
    48901 tatggatctc ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg
    48961 gttgattctg tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg
    49021 atatcaacat ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca
    49081 tcccgatctt ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca
    49141 accgtatctt tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt
    49201 agctgacgga tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc
    49261 atgttttcag cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc
    49321 gctccattta gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag
    49381 atagccaagc catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc
    49441 atccgtttat gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca
    49501 tgtggaagaa ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac
    49561 agctttttca ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg
    49621 ctcctgcaag cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc
    49681 ctgtagcagg caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat
    49741 ttgtctccaa aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt
    49801 ttggataata cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat
    49861 cattttctat gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt
    49921 ttctatagct gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg
    49981 aagcattgaa tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat
    50041 actctcctgc actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat
    50101 tcatactgtc aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta
    50161 cttgtagtac accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga
    50221 tgaccgtatc ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat
    50281 cagatagccg atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca
    50341 gatgcagagg ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat
    50401 agaagtatcc gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt
    50461 atgctcgata cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat
    50521 ctaatgtatg gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat
    50581 tgtaagcagt gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt
    50641 gccaattttc ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc
    50701 gcaagatcca tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa
    50761 tgattttgta ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg
    50821 gctctccgat tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat
    50881 ctactgaata gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc
    50941 ctttcgctac tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact
    51001 ctgcgccatt gattggcata ttactgcctt caattagttt attgatattc atgtggattg
    51061 gcttgaattc atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta
    51121 tgttaggctc ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat
    51181 ctttaccttc cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt
    51241 gacctaacat ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat
    51301 ctactaactg ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg
    51361 ctccagtttc atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg
    51421 tagttttact tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg
    51481 caaattcgac tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac
    51541 ggcctttatg ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat
    51601 cattctcata atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc
    51661 cgtcttgagc tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac
    51721 ctaaagaaat cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag
    51781 tcgccactac ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt
    51841 ggtacttcat gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc
    51901 cagctccaaa tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg
    51961 ttctcttgaa ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg
    52021 cattgtaata tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat
    52081 ttcgtccaat agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa
    52141 tggagaagat acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa
    52201 ctgtgttgat cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag
    52261 aagaaccatc accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac
    52321 gatagtgtac atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat
    52381 gagtaaaccc tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt
    52441 cattatcttc agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc
    52501 tcgtacgata acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa
    52561 cgattaggat tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat
    52621 agccattttt aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg
    52681 tattctcact tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga
    52741 tttgctcatg gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa
    52801 tatccagact cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg
    52861 cagcattttt agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat
    52921 ctagaacaaa aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta
    52981 gatagacatt gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg
    53041 gcagaacagg ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt
    53101 caaaattccg ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt
    53161 cgcctgcagt tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt
    53221 ttgtataatc actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt
    53281 gatagcgttc attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa
    53341 acttcgggta gcgatgctgt tcgttttcaa cttctccgct aggatagata tattcatagg
    53401 ggtcttgcgt agctgttggc ccaagacgct ccaaaatacc gttgaataac tggcgttgaa
    53461 tgcgctggag tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc atctcatttt
    53521 cttcgctacg ttgaatttca gcggaatctt ctgtatcagt ttcattttct tgtaattctt
    53581 ggttatctat tctttcatca accaccactt gaagaggtag ttacccttcg ttcacatcgg
    53641 ggatcttttc tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa taaacttttc
    53701 cgacatacca ttcttgacca tcgatcgtct cgacttttag gtcttctcca tcttcatcat
    53761 aggaggatag acctgttcca cgaacgtttt ttacggtacc aatcccggcg gctgccgcat
    53821 cgattgctaa acgtattcta ccttcattgg cactttcctt tttttcaaaa gccagatgcc
    53881 attcaagggt ttcttctttt tcaatatact tcccttcaat ttttgcatag tcctcatcta
    53941 agaatacctc atcctccttt gtttctgttg actcagcagc tacatccagg gtcgtcatat
    54001 gatagactga ctggaaatat ggtccaaata gcaaactgac agttactaac tgcaaaagct
    54061 tcaattttaa ccctttcaca tccttatact cctttcaaaa atatttgtct tctttcacaa
    54121 caatcaacaa aacaatgccc atctaattat aatttaattt aattttaatt ttaaatcaaa
    54181 tgatatcctt tttagttaaa aaacaccaat tatacaacat ttattattaa aattaaatta
    54241 taaaatttta taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca
    54301 actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt
    54361 tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa
    54421 tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga
    54481 tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag
    54541 ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac
    54601 ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt
    54661 tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat
    54721 attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac
    54781 cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt
    54841 gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat
    54901 tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa
    54961 aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca
    55021 agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag
    55081 aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta
    55141 aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt
    55201 cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt
    55261 gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt
    55321 gaaccgagct ttttctcttc atttttttgt aatgataaat catatacaga actgttcgac
    55381 ttgcagttcc taatgtgaaa acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga
    55441 taagacgtgg aatccattca atgatttcta agaaataatc attggctttt ttaccaatcg
    55501 atcgattcat catccttttg aacctcctgt caaatccatc ccttcaatga aataacgttg
    55561 tgcaaaaaag aacagcaaaa tcgtcgggat caagacaagt gttgctcctg ccatcagata
    55621 attccattgt gtcgttgctt ggttctgaaa aacttgcaag ccaatctgta acgtgtattt
    55681 ttcttgatct tggatatata acagaggacc taagaaatca ttccaagctg cgttgaagga
    55741 attgatcgca atggtaatca aggcaggttt cgtcaatgga agcatcaaat gactccaaat
    55801 atacaaatga ttcgctccgt cgatttcagc agcttcgatc aattcattat tgatcgataa
    55861 gtaaaattga cgcatcagaa atatattgaa cgcattgccg aagaacgatg gaacgatcag
    55921 gggcaaataa gtacctaccc agccgatctt ggagaataat acatattgag ggatcatcgt
    55981 tacaaacccc gggatcatca tcgtagaaag aaccaaagca aacatcagct ttttcccagg
    56041 aaaatcaagc ttcgcaaatc cataagcaat aaaagaattt gaaaggacat taccgatcac
    56101 tactagcaca gtgatgaata atgtatttct tgcatagcgc caaaaaggaa aggcggcaat
    56161 cgtatccaga tagttttccc atacgatttt ttctgggaaa aaggtcggag gataacgaac
    56221 gatttcttcc attggtttca atgaagtaaa caccatccaa agcaatggaa tgatcaaaat
    56281 gattcctatt actgtcatga ggacataact tagtattttt ttgtttgccg attcacactg
    56341 gtctttttga aataatccat catttgccgt cacctccaga ataatagacc cacttaggtg
    56401 caagcttcaa attgacaaac gtcagaacca acacgatcag aaacaggatc attgacatag
    56461 ccatcgcata acccatatca aaagctacga acgccttgtt ccacatatgc agattataaa
    56521 aaagcaaaga atttgcaggt ccgcctgttc catttttcgt catgacataa gcttcttgaa
    56581 aaatttggaa agaaccgatc gtagaggtga tcacatcgta aaaaataata ggagtgatta
    56641 atggcaaagt aatacttttg aactttcccc aaacgcctgc tccatcgatt tctgctgatt
    56701 catataaaga tctaggaaca ttttgtaatg tagctaagta aagtagcatt gctcctccag
    56761 aactccatat tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca aacagccaag
    56821 ctggtccagg gatatggaac cagcccagaa atgtgttgat caatcctgca gatggagaaa
    56881 gcagctgcat ccataagatg tagactgcta ctcctgataa gactgccggt aaatagaaag
    56941 ctgtacggaa tacactgatt cctttgattt tttgattgag tactgtggct aaaaacacac
    57001 tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata aacggtgttg tataacgatt
    57061 tccaaaataa atcatcgtga acgaacattc ttttgaaatt tgctagacca atgaaattca
    57121 tcttggatgt caaattataa tcggtgaaac taccaataac tgaaaaaagc ataggcccta
    57181 gcataaatac caagaacccg ataataaaag gcgagataaa taaataggcc catcttgctt
    57241 cttttttctt tcggatagat aaacgcttca aattttcttg cctcctctgt acctagacag
    57301 tcaaaaatgg attattgatt ttgcttgact aagttttcta ctgctttttg tgcatctgcc
    57361 aatccttctt ttggtgattt tgttcctaaa agaatctggt caatctgttc attcactaat
    57421 gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca ccgtattttt aaagttttcg
    57481 tccgccattt gatagatcat ttttccttta tccgttaggt tgtcattatt cactaaattc
    57541 tcattggctg tcttgctcgc catgatatca aaactttttt cgccgaattt ttcttgtact
    57601 tctggagaag aaagatattt gatgaattca taagaggcct caggatcttt tgctccgtat
    57661 ggtacttcca agacgaatcc gccaccccac gaccaatggc ctgaaccttc ttctttttct
    57721 gggagctgca tcacgccaaa atcaaaatca tccggtgcat tttccatcaa actagagtaa
    57781 taattgatat tttgggcacg catggctact aatccggaaa taaatggatc agatacacca
    57841 gacccaaatt cggcttctaa tttattgatc gtatttttcc cataataatc ttgccagtct
    57901 tggatccact ccaaggcttc cactttattt ttagtatcaa tcttgacttt ttcatctttg
    57961 tcaaaccaac tagtcccgtt atctgcgttt agcgcccata catcagcccc taaattccat
    58021 aatggataaa aaccgatgcg tgtaaattct tttccttctt ttttatctag tttacgtgct
    58081 gtttcttcta attcatccca agttgtcggt agatcttgtt cactgattcc agcttcttta
    58141 aatatctttt tattataaaa aatgacttgt gtatctgtat tgaatgggat cgcataagga
    58201 tcaccttcat attctactgt atcccaaagc tgcggataaa aatccttaga agtgtctttt
    58261 tctacatatt tggagagatt agttgcttgc tgtgcttctg ctctttgggc aacggaattt
    58321 atgtcctgta caataacatc gggtgcattt ccagctgtta ttgcagataa agatttagtc
    58381 cagatgtcac cccatggctg atacttataa tttacatgga tctcgttttg tgattgattg
    58441 aaatcctcaa tgatttcttc gatagcttct tttcgtgcac cggatcccca aaaggaccag
    58501 aaatcaatat tttttcttcc atcacttgtt ttttcagaag aatttgctgc acttgaactg
    58561 gcatcgcttg cattacttga tccgcatcct cctaataaaa acaatgctga acttgccagc
    58621 aaccctgtaa acaataattt tttcatattt tacctccaaa ttttaatgta accgatgccg
    58681 gttttaatat aacgatatca ttttgttctg aaaataacaa tttataaggt atgattctaa
    58741 aaaaattatt aatgatacca atactttata taattcgcga gcgctttctt tgaatttttt
    58801 aatctttcgt agcagaatga gaatgtatta aaaatagatt aggaggaatt ttgatgttta
    58861 gacaccagaa agaattgcag tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga
    58921 aatatctgca ggagctgatc ggcggccaat atggcgagat gagtgtagct atgcagtacc
    58981 tgttccaagg ctggagctgc cgaggagaag aaaaatataa agatatgctg atggacattg
    59041 caacagaaga aatcggccac gtggaaatgt tggcgacaat ggttgctcgt ttattagagg
    59101 gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta
    59161 tctctggaat gaatccacag cacgcgatcg tagctggctt aggacctcgt cctgctgaca
    59221 gcgtgggcaa tccatggagc ggtgcctata tcattgcgag tggcaacttg ttagcagact
    59281 ttagagctaa tttaaatgcc gaatcacaag gaagacttca agtagcacgt atttacgaaa
    59341 tgatcgatga tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga gatagctatc
    59401 atcaaaatct atgggaatgt gcagtgaaag aattagaaga aaaagaaggt tcaatcttag
    59461 tcccaagtac tttcccgcgt gacaaagaac gaaaagacat tgcttatgac ttctataact
    59521 tctcagaggg agaccaaagt agtgaaggtt cttgggctag tggcaaagca ctggatggcg
    59581 aaggcgaata tcaatggttg aaagaaccta agatcgaagg cgctgcacca aaattagaac
    59641 cagtaacacc aaaaatgtat gggacacctc cattcaagaa ataaatatct acccgtagaa
    59701 acacccgttt tttgttccac acgctttgtg agcaaaactg actaaagtca ttaatcataa
    59761 ccacccttca aaagggtggt tttctctaga gctataagct caaatcaccg gccagcgcct
    59821 aaagacgctg gctttcatat tttatgttca agccatattg cctttgccag ttttgcccac
    59881 ctcttaaaag gtttatgtac tactccttag cccttaaaag gatcgtctta ctctcgtaca
    59941 ctcagcttat ctaacgcact atcatgtttt tcttgttctt gaatgtactt tttgatggtc
    60001 gcttcatttt gtcctacttt tgtcacatag tatccttcca cccaaaagtg tcgataccca
    60061 aatttatatt ttagatttgc acaatctctt gatgatctcc tgaatattgg ctcgatattg
    60121 attataaatt atttttcttc tatactttgg tgtgaataca atgtggtatt tacacatcta
    60181 ctttgtacgg gctaaactat ttccctttat tgctccaccc gcatagcaag tggttttgta
    60241 tttcaatcgc tcggcgcgct caattaggtc taaagacaat aaatctaaaa acccgctgct
    60301 tcactgacag aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca
    60361 atctatttta tttttttgat tttttataaa tggaaaatgc ttcaagaagt ccaagaaata
    60421 aagccgtata aaaacaagaa cctatgaaat ctttatttat tagatattca acagaaatcc
    60481 cgataacact agccaacaat atatttatca gaagtgtaaa ataaggatgc tttttataga
    60541 atagaactag tgttgttttc atagactctc ctttcttcat atttccaatt aatcacaaat
    60601 tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc
    60661 cctacaaacc aggccatctt gtgtttaata actataccaa atctaaggta cctctaaatc
    60721 taaattccat ttgtggcggt gacctccaat ttttaactct tctatctccc atccagtata
    60781 cataaccttg atacttcgac cacttatata tcgaacacac aaatacctat ccattgcagg
    60841 agtactacct aacttagttg ctacttgcat ctaaataaca gctgaaaaga taggacttac
    60901 tcttagtaaa gtatctgaac tattaatttt taaactatca tttttatcct caactacatg
    60961 cacatctgtt gcaacttcac tttctgtctc atccagataa atgattaagg aatcgggaat
    61021 tgcaatatct tcagctgacg ctggaatagt tgagctaaat tagtcatcat atctttatta
    61081 taaaataa
//
LOCUS       DAYYBH010000010        55927 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000010 DAYYBH010000000
VERSION     DAYYBH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.010"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(30..395)
                     /locus_tag="ACHAUG_000697"
     CDS             complement(30..395)
                     /locus_tag="ACHAUG_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479811.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            603..1085
                     /locus_tag="ACHAUG_000698"
     CDS             603..1085
                     /locus_tag="ACHAUG_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479812.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1282..2172
                     /locus_tag="ACHAUG_000699"
     CDS             1282..2172
                     /locus_tag="ACHAUG_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8479813.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2497..4968
                     /locus_tag="ACHAUG_000700"
     CDS             2497..4968
                     /locus_tag="ACHAUG_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8479814.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5071..6807)
                     /locus_tag="ACHAUG_000701"
     CDS             complement(5071..6807)
                     /locus_tag="ACHAUG_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8479815.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6804..7508)
                     /locus_tag="ACHAUG_000702"
     CDS             complement(6804..7508)
                     /locus_tag="ACHAUG_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8479816.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7639..8142
                     /locus_tag="ACHAUG_000703"
     CDS             7639..8142
                     /locus_tag="ACHAUG_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8479817.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8102..8440
                     /locus_tag="ACHAUG_000704"
     CDS             8102..8440
                     /locus_tag="ACHAUG_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8479818.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8461..9879
                     /gene="ffh"
                     /locus_tag="ACHAUG_000705"
     CDS             8461..9879
                     /gene="ffh"
                     /locus_tag="ACHAUG_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8479819.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9991..10569
                     /locus_tag="ACHAUG_000706"
     CDS             9991..10569
                     /locus_tag="ACHAUG_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8479820.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10573..11094
                     /locus_tag="ACHAUG_000707"
     CDS             10573..11094
                     /locus_tag="ACHAUG_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8479821.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11173..11727)
                     /locus_tag="ACHAUG_000708"
     CDS             complement(11173..11727)
                     /locus_tag="ACHAUG_000708"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8479822.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11980..12255
                     /gene="rpsP"
                     /locus_tag="ACHAUG_000709"
     CDS             11980..12255
                     /gene="rpsP"
                     /locus_tag="ACHAUG_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8479823.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12267..12518
                     /locus_tag="ACHAUG_000710"
     CDS             12267..12518
                     /locus_tag="ACHAUG_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8479824.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12643..13434
                     /locus_tag="ACHAUG_000711"
     CDS             12643..13434
                     /locus_tag="ACHAUG_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479825.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13604..14125
                     /gene="rimM"
                     /locus_tag="ACHAUG_000712"
     CDS             13604..14125
                     /gene="rimM"
                     /locus_tag="ACHAUG_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8479826.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14115..14876
                     /gene="trmD"
                     /locus_tag="ACHAUG_000713"
     CDS             14115..14876
                     /gene="trmD"
                     /locus_tag="ACHAUG_000713"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8479827.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15012..15359
                     /gene="rplS"
                     /locus_tag="ACHAUG_000714"
     CDS             15012..15359
                     /gene="rplS"
                     /locus_tag="ACHAUG_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8479828.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15491..16627)
                     /locus_tag="ACHAUG_000715"
     CDS             complement(15491..16627)
                     /locus_tag="ACHAUG_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8479829.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(16743..17309)
                     /locus_tag="ACHAUG_000716"
     CDS             complement(16743..17309)
                     /locus_tag="ACHAUG_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFM8479830.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(17445..18098)
                     /locus_tag="ACHAUG_000717"
     CDS             complement(17445..18098)
                     /locus_tag="ACHAUG_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479831.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(18148..18576)
                     /locus_tag="ACHAUG_000718"
     CDS             complement(18148..18576)
                     /locus_tag="ACHAUG_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8479832.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(18581..18955)
                     /locus_tag="ACHAUG_000719"
     CDS             complement(18581..18955)
                     /locus_tag="ACHAUG_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8479833.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            19259..19444
                     /locus_tag="ACHAUG_000720"
     CDS             19259..19444
                     /locus_tag="ACHAUG_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8479834.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            19457..19705
                     /locus_tag="ACHAUG_000721"
     CDS             19457..19705
                     /locus_tag="ACHAUG_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479835.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            19736..19906
                     /locus_tag="ACHAUG_000722"
     CDS             19736..19906
                     /locus_tag="ACHAUG_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479836.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(20008..20475)
                     /locus_tag="ACHAUG_000723"
     CDS             complement(20008..20475)
                     /locus_tag="ACHAUG_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479837.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            20634..20951
                     /locus_tag="ACHAUG_000724"
     CDS             20634..20951
                     /locus_tag="ACHAUG_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479838.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            20884..21363
                     /locus_tag="ACHAUG_000725"
     CDS             20884..21363
                     /locus_tag="ACHAUG_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFM8479839.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            21380..21556
                     /locus_tag="ACHAUG_000726"
     CDS             21380..21556
                     /locus_tag="ACHAUG_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479840.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            21813..22244
                     /locus_tag="ACHAUG_000727"
     CDS             21813..22244
                     /locus_tag="ACHAUG_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479841.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            22250..22567
                     /locus_tag="ACHAUG_000728"
     CDS             22250..22567
                     /locus_tag="ACHAUG_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8479842.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            22686..23372
                     /locus_tag="ACHAUG_000729"
     CDS             22686..23372
                     /locus_tag="ACHAUG_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8479843.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            23347..24696
                     /locus_tag="ACHAUG_000730"
     CDS             23347..24696
                     /locus_tag="ACHAUG_000730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8479844.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            24687..25160
                     /locus_tag="ACHAUG_000731"
     CDS             24687..25160
                     /locus_tag="ACHAUG_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8479845.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            25162..25662
                     /locus_tag="ACHAUG_000732"
     CDS             25162..25662
                     /locus_tag="ACHAUG_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFM8479846.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            25677..27989
                     /locus_tag="ACHAUG_000733"
     CDS             25677..27989
                     /locus_tag="ACHAUG_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM8479847.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            28236..28553
                     /locus_tag="ACHAUG_000734"
     CDS             28236..28553
                     /locus_tag="ACHAUG_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM8479848.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            28550..28711
                     /locus_tag="ACHAUG_000735"
     CDS             28550..28711
                     /locus_tag="ACHAUG_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8479849.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            28714..29013
                     /locus_tag="ACHAUG_000736"
     CDS             28714..29013
                     /locus_tag="ACHAUG_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479850.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            29013..29327
                     /locus_tag="ACHAUG_000737"
     CDS             29013..29327
                     /locus_tag="ACHAUG_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8479851.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            29320..29850
                     /locus_tag="ACHAUG_000738"
     CDS             29320..29850
                     /locus_tag="ACHAUG_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8479852.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            29847..30116
                     /locus_tag="ACHAUG_000739"
     CDS             29847..30116
                     /locus_tag="ACHAUG_000739"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479853.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            30046..30249
                     /locus_tag="ACHAUG_000740"
     CDS             30046..30249
                     /locus_tag="ACHAUG_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8479854.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            30246..30533
                     /locus_tag="ACHAUG_000741"
     CDS             30246..30533
                     /locus_tag="ACHAUG_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479855.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(30566..30817)
                     /locus_tag="ACHAUG_000742"
     CDS             complement(30566..30817)
                     /locus_tag="ACHAUG_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479856.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            31020..31433
                     /locus_tag="ACHAUG_000743"
     CDS             31020..31433
                     /locus_tag="ACHAUG_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8479857.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            32053..32217
                     /locus_tag="ACHAUG_000744"
     CDS             32053..32217
                     /locus_tag="ACHAUG_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479858.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            32214..32444
                     /locus_tag="ACHAUG_000745"
     CDS             32214..32444
                     /locus_tag="ACHAUG_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479859.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            32629..33174
                     /locus_tag="ACHAUG_000746"
     CDS             32629..33174
                     /locus_tag="ACHAUG_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8479860.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            33271..33492
                     /locus_tag="ACHAUG_000747"
     CDS             33271..33492
                     /locus_tag="ACHAUG_000747"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8479861.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(33649..33813)
                     /locus_tag="ACHAUG_000748"
     CDS             complement(33649..33813)
                     /locus_tag="ACHAUG_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM8479862.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            33915..34436
                     /locus_tag="ACHAUG_000749"
     CDS             33915..34436
                     /locus_tag="ACHAUG_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8479863.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            34423..34863
                     /locus_tag="ACHAUG_000750"
                     /pseudo
     CDS             34423..34863
                     /locus_tag="ACHAUG_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(34903..35061)
                     /locus_tag="ACHAUG_000751"
     CDS             complement(34903..35061)
                     /locus_tag="ACHAUG_000751"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM8479864.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            35154..35915
                     /locus_tag="ACHAUG_000752"
     CDS             35154..35915
                     /locus_tag="ACHAUG_000752"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8479865.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            36102..37454
                     /locus_tag="ACHAUG_000753"
                     /pseudo
     CDS             36102..37454
                     /locus_tag="ACHAUG_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            37470..38690
                     /locus_tag="ACHAUG_000754"
     CDS             37470..38690
                     /locus_tag="ACHAUG_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8479866.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            38725..39402
                     /locus_tag="ACHAUG_000755"
     CDS             38725..39402
                     /locus_tag="ACHAUG_000755"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8479867.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            39399..40592
                     /locus_tag="ACHAUG_000756"
     CDS             39399..40592
                     /locus_tag="ACHAUG_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8479868.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            40607..40933
                     /locus_tag="ACHAUG_000757"
     CDS             40607..40933
                     /locus_tag="ACHAUG_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8479869.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            40937..41272
                     /locus_tag="ACHAUG_000758"
     CDS             40937..41272
                     /locus_tag="ACHAUG_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8479870.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            41269..41664
                     /locus_tag="ACHAUG_000759"
     CDS             41269..41664
                     /locus_tag="ACHAUG_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8479871.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            41661..42032
                     /locus_tag="ACHAUG_000760"
     CDS             41661..42032
                     /locus_tag="ACHAUG_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479872.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            42048..42608
                     /locus_tag="ACHAUG_000761"
     CDS             42048..42608
                     /locus_tag="ACHAUG_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8479873.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            42733..43044
                     /locus_tag="ACHAUG_000762"
     CDS             42733..43044
                     /locus_tag="ACHAUG_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479874.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            43068..43259
                     /locus_tag="ACHAUG_000763"
     CDS             43068..43259
                     /locus_tag="ACHAUG_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479875.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            43259..47107
                     /locus_tag="ACHAUG_000764"
     CDS             43259..47107
                     /locus_tag="ACHAUG_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8479876.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            47108..47980
                     /locus_tag="ACHAUG_000765"
     CDS             47108..47980
                     /locus_tag="ACHAUG_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8479877.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            47977..49026
                     /locus_tag="ACHAUG_000766"
     CDS             47977..49026
                     /locus_tag="ACHAUG_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479878.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            49030..49854
                     /locus_tag="ACHAUG_000767"
     CDS             49030..49854
                     /locus_tag="ACHAUG_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479879.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            49872..51932
                     /locus_tag="ACHAUG_000768"
     CDS             49872..51932
                     /locus_tag="ACHAUG_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8479880.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            51949..52098
                     /locus_tag="ACHAUG_000769"
     CDS             51949..52098
                     /locus_tag="ACHAUG_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479881.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            52095..52541
                     /locus_tag="ACHAUG_000770"
     CDS             52095..52541
                     /locus_tag="ACHAUG_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479882.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            52543..52680
                     /locus_tag="ACHAUG_000771"
     CDS             52543..52680
                     /locus_tag="ACHAUG_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8479883.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            52715..52957
                     /locus_tag="ACHAUG_000772"
     CDS             52715..52957
                     /locus_tag="ACHAUG_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFM8479884.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            52973..53170
                     /locus_tag="ACHAUG_000773"
     CDS             52973..53170
                     /locus_tag="ACHAUG_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8479885.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            53181..54197
                     /locus_tag="ACHAUG_000774"
     CDS             53181..54197
                     /locus_tag="ACHAUG_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8479886.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            54312..55520
                     /locus_tag="ACHAUG_000775"
     CDS             54312..55520
                     /locus_tag="ACHAUG_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479887.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            55504..55674
                     /locus_tag="ACHAUG_000776"
     CDS             55504..55674
                     /locus_tag="ACHAUG_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479888.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT        19109 a         9541 c        11816 g        15461 t
ORIGIN      
        1 gcttctcttt gtacatatat ggtttgtttt tatggatgcc gagctttttt ataagataag
       61 gctaccgccc ataacgaccc aaggatttgt ggaactaagt aaatacctgc aagaagcaag
      121 atcagctggg tcggttttaa aggatgatag aagtaacgcc attcagtaaa attggcgcaa
      181 aataaatagg aagtgacagt agatataata ttggcaatga ataaagatat aatacgcctg
      241 ttcctttgaa aataatatcc agcaaaaaga gataaaaata tcaatgtgat gaacccgctt
      301 acggaatgat aacgaaaatc aagcagtaag gcgaaatgca cataaggaac aaaagataaa
      361 agcaacggaa ggtttttcca gcaagttttt atcatctttc atcaccacct aacaagtgtt
      421 attattttgt tagttttatt gtaaattgaa tcctgcaata atcaattact ttttcattat
      481 ttctagatta aatgaataaa ctctcatgcc attatctcta aaaaaaaagt ttttccccct
      541 aattttttta gatagaaaat tgtatagtat gatgagagaa aaagtagttt gggggaacgt
      601 taatgagaag aaaagcagta atcgatcggc tttatttatt tttttggttt tttcctatcg
      661 ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg tgcggagata ttaggcctga
      721 tccaaggaag cggatttgtg gtttctagga attttccagc tttgactgtt ttctatttag
      781 tgataagtgt aggtctctta ttaccaaaat ctctgatcaa tcactacaat tggcagctgc
      841 tttacttttc ttgcttatta gtatttacat ctatgttttc tttgctgcct cgcttgatta
      901 tcggccctaa tggtttgagc gagagtggat tgaaggattc agcttattcc tccatgtatt
      961 tcttgacgat caaaccatgg ttctatttag taattgcctc atttattctt gcaacatttt
     1021 tttacttctg gacagaaatc aggataaaaa gtgaacgatc tgtatctata aaaaataact
     1081 attgatggtg gatcatcagt aaaaaagcat atgagctgcg taaaatgcat gtttctcata
     1141 tgcttttttt attattatgt aaagaatgcg aaaaacgttt acatgatttt tacataactt
     1201 aactatattt ttacagcaac catacgaggg atttacagtc gactgataaa ctttagttgt
     1261 aaataagaaa ggagcaataa aatgaaaaaa gtattgttta ctctaggtat gatgggaatt
     1321 cttttagccg gatgcggctc aggatcagga aatacagatg gctctgccac acaaaataat
     1381 tcaagtgact caaatgaaca agtaaaaatc gttgcggtag gttcgacagc attacagccg
     1441 ttagtcgatg cagcacaaga aagctttgta caagaaaatc caaattatca aatctctgta
     1501 caaggaggag gaagcggaac tggtttgagc caagtcgaag ctggagcagt tactatcgga
     1561 aactctgatg tatttgcaga agaaagagac ggagttgatg cttcaaaatt agtggatcat
     1621 aaagtagcag tggtcgggat ggcgccaatc gtcaataaag atacagatgt caaagatatc
     1681 accaaacagg aattgattga tatctttaca ggaaaaatca ctaactggaa ggaagttggc
     1741 gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg gcagcggtac ccgcgcgaca
     1801 ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt cccaagaaca agattcatca
     1861 ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa tcagctattt agctttctca
     1921 tatctcgatg attccacaca agcactaagt atcgacggtg tagaaccaaa agaagagaat
     1981 gtagcagata acagctgggg aatctggtca tatgaacata tgtacacgaa cggaaaacct
     2041 tcccctgaag tccaaaaatt cttagactac atgatgacag aagaaatcca agaaggacct
     2101 gtcaaagaat tgggctattt accaatcaca atgatggaag tagaacgtga tcacgaagga
     2161 aatatcaaat aactagcaga taaacatgcc acggtagtcg ttactattaa ggaaaaatca
     2221 actatgtttc ccacatatac atttttcctt taattgtgaa aaatagttcc tcatattttg
     2281 ccgttttttg agctaatacc gagggtcagc tgattaaaca ccgtttattc gaaaaaaaga
     2341 gctacgacat agaactgtcg tagctctttt tttgctatat gacaggaatg aaaggtgttt
     2401 gcaagcttaa tgtgaggaac actttccata tctgttctat actggatagg agaagtttgg
     2461 aaatatccag aaaacgaaat caatcgaggt agccacatga agaataaatg gataaatcga
     2521 agtaaaaaaa agaaagtaac acaaagatca tcagataaag tcatccgtct ttttccatcg
     2581 tataaagaag gattaacggc agatcaagtc aaggaacgaa tagaaaaagg tgcagcaaat
     2641 aattcagtcg atcccacatt caaaacaaat cagcaaatcg tattggaaaa catatttacg
     2701 tatttcaatt tgatctttct tatcttagcc attttgcttt gtttagtaga atcctataag
     2761 aacttgactt ttttaccagt tatcatagcc aatacaggaa tcgccatttt tcaggaaatt
     2821 cgatccaaaa agatcttgga cgaactaaat gttttaaata cagcaaaagt caatgtcata
     2881 cgggacggga tagaacaaac aatagatata gaagaccttg taatagatga cattgttatt
     2941 ttagagactg gacatcaaat tccagcagat gcagaggtcg tggaaggaaa actgcaagtt
     3001 aatgaggctt tattgacagg cgaagcagat gaaataacca aagaaatagg cgatccatta
     3061 atgtccggca gtttcattgt ttccggaaaa gcttacgccc gattagacaa agttggagca
     3121 gattcgtata tttcccagct gacagtcaaa gccaagacaa tgggagaagg agaacagtcg
     3181 gagatgattg cttctcttaa tcaattgatc aaatggattg ggatcatcat tattcctatc
     3241 ggtatcctgc ttttttctca aagttacttt tttaatggaa acacgatcaa agaaagtgtc
     3301 gttgcaatgg aagcagcatt gatcggtatg atacctgaag gactgtactt gctgacgacg
     3361 attgcattag cattaagtgc gactaaacta gcaaagcaaa gagttctttt acacaatatg
     3421 aaaagcattg agacattggc aagggtaaat gtcctttgtg tagataaaac aggaacgatc
     3481 acagaaaata aaatgtctgt tcaaaaagtg atcgtctcta aaaaacaaga aataatcagt
     3541 cagtcagatc aattggaagc atgcattgct gactatgcaa aagcgatggc aggagacaat
     3601 gcaacaatgg aagctgttaa agcatatttt acaaaaacca ctgatcaatc ctatcaccat
     3661 gttattcctt tttcttctgt ccaaaaattc agcagtgtag cgttggcaga caaggtgtat
     3721 gttttaggag cgcccgaaat ggttttgcgc gatcaattgc cggaatatgc ggaagagttt
     3781 gtttctttcg ctgaaaaagg ctatcgcgtg ttggtctttg gtgtgtacga tgggattctt
     3841 gaagaaccag tattgacaga agcagtcaca ccattaggct atattctgat tgctaatcct
     3901 atcagaaaag aagcaagagc cacatttgat tacttcaaaa aacagcaagt agccatcaaa
     3961 gtgatttctg gagataatcc gctcaccgta tccaatgtag ctgtccaagc agggatttca
     4021 catgctgatc agtacgttga tgcatctaaa ttatcagaag aagaatatga agcagctgtc
     4081 gaaaaatata cagtttttgg acgcgtcaag ccagaacaaa agaaaatctt cgttcaattg
     4141 ataaagcgta acaatacagt agctatgaca ggtgatggcg tgaatgatat tctagccatg
     4201 aaagaagcag actgcagtat agcgatggct tccggaaatg aagccgccat gcaggcctca
     4261 caagtggtct tacttgattc tgacttttca cgcatgccag aagttgttgc agaaggacgg
     4321 cgcgttgtga acaatattga gcgatcagcc agcttgtttt tagtgaagaa tattttctcc
     4381 tttttgctct cactgttttc agtgatcttt gctctgacct atcctttaga accatcacaa
     4441 atcacattga ttagtttgtt tactatcgga cttccatctt tcttgcttgc tctagaagaa
     4501 aacaaaaagc gaatcagagg gaaattcatc atgaatgtga tggaaaaagc tgtgccaggt
     4561 ggactaacag atatgatcgt agtcggtgcg ttggtgatct gcggggtgac tttgaatcta
     4621 aataaaacag atgtttctac agcatctacc atgcttttga ttgcagtagg gttcctcgtt
     4681 ctctataaaa tctgttcacc gctgaataaa ttcagaagtc agatcattct tttctgtgct
     4741 agcggtaact ttttttcggt catctttttg cataagctgt tttcaatcac cggtatctca
     4801 gctgtatcac ttttgctgtt gagcattcta tttttctcgg cagattcgat ttttaggcat
     4861 ttgaccacat tggtagaata tttgtttgaa cgtaaaaatg aaaaagagca aaagccgttt
     4921 agtcgcgtag ctaaaaaatt tctatcgata tttaaacgag aaaattgacg aaaagtatct
     4981 caaaaaagag taaaaaagaa caaccactcg tgtatctttc gaagcaggat gagtcatcca
     5041 actttaaaac gcacgagcag ttgctctttt ttacattttc ggaatggtaa cggtgaaagt
     5101 cgaacctgtt ccaagatgac tgtctacagt aatcgtacca ttaaggagtt gcacgtagtc
     5161 ttttacaatc gctaacccta atccggtccc gcctgaatgt cggcttcgcg ccttgtctac
     5221 gcggtaaaaa cgctcaaaga tccgttcctg atcctcaatg tcgataccta taccaaagtc
     5281 ttggacagaa aacgatagat catctgttgc ctgatagcgg ataataatct cgctgtcgct
     5341 ttttgaatat tggacagcat tttcaatcag atttttgata attggataaa ggatatctgt
     5401 tttagtagta aatggttcgt tctttccaat caatcgaatc gtcaaccgtt tcttttcgat
     5461 gattggctga taattttgaa cgatttgctg gaaataaggt tctgtataaa ttgtctggat
     5521 ctcgtaagaa ttctcgccat cttttgacag ctggatgatt tcccgaatca gtttatctaa
     5581 ccggatcgca tctttttgca tgatctcaag aaaagaagtc agagtttgcg ggtcatcttt
     5641 tgcaccatcc agtaaagtct cggtaaaacc aatcagtgaa gtcacaggag tttttaattc
     5701 gtgagagacg ttgccaacga aatccttttg tagcttttct aaacgtcgca ctttcgttaa
     5761 gtcatacgcc acccctagaa tctgtccggt accttctgaa ttagtaaagt agcgcaaact
     5821 caagtctaat gtctgccgac cttctgtaag ggtgatctcc tgatggatca gcggatgctc
     5881 aggagtcact tgatgaatca gctgaatgag ttttggctct tgaatgactt ctgtgtaccg
     5941 ctgttcaggt tgatagctta ccacgtttaa atgtagctgc atctttggat taagcagtag
     6001 taaacgagaa tcagcatcat caatcaggaa tacgccaatc atcagttcgt tcagtaacgt
     6061 atataattgc tcttctgtgg tagtacacgc ccgatacatc ttactcattt gttcgctcaa
     6121 cgcattaatc gtctgataca attcttccca ttgatcgggt gtttgcatga ttacttccgc
     6181 ctgattagga ttttgcacca ttttttttag tacgggcagc acggttttca atggctggtt
     6241 tttttgatag atcagataat agatcattcc tgcaagaaca aggaagaaag acagaaagaa
     6301 gaaaaagacc caacggcgaa aactctcagt tctcggtaaa aaaccgcttg tcggttctgc
     6361 gatacgtaaa atggcttcta actggccact ttttttgact ggtaatgcga catataaaag
     6421 atcctcattc aaagtagtac ttttgcgcaa agcgctacct aggttcccac cattcaatac
     6481 ggctttgatt tcgggacggg agtttcgctg ttcattcaat gtttcattgt ttgtgtcaaa
     6541 taagatcttt ccagtagcat ccattaaagt aatccgttcg ttcgactgat ggacgaattc
     6601 ttccaaagca gttttgtttt gcgctgcttg gaggttttcg acatctaatt gccggatcag
     6661 cagttgtgct ttttttgtca ggtagctttc ttgctgttcc acgatctgct gttgaaaata
     6721 atgggaaatc aattgccagc caccaaaaaa caaactaact aaaagaataa gaaagaagag
     6781 acgttcataa atttttttcc atttcatcgt tttggttcct gaaacttata gccaaatcct
     6841 ctaatagtca ccaagtactt tggatgtttt ggatctcgct cgattttttc tcgcaaatga
     6901 ctaacgtgta catctacgat tcggctttgt cctgcgaagt caaaattcca aatgcggtct
     6961 agcaatgtgt ctcggtcgat cacacggtct tttcgtttca tgaaataaac aagtaattca
     7021 aattctttcg gtgttagttc aatcagatgg tcgtctacgg tcacttgata attggtcaaa
     7081 tcagcagtga tttgtccaat cgatagatat tcaggctcag cttcatcttt tgattgctta
     7141 cgaggttcaa tccgtctgaa tattgctttc atacgggcta atacttcgcg aggactaaat
     7201 ggtttggtca aatagtcgtc agcacctatt tctaggccaa tgatccgatc cacttggtca
     7261 tctttagccg tcagcatcaa aatcggtgtt tcattttttt cttgacgcag ccgctgagtg
     7321 atctccatgc catccatcgt tggaagcatc acatctaaaa tgatgaaatc aaatgattgt
     7381 tctaatgcta attctaatcc ttcgcctcca tctgtggcac tggttacctt gtagccttct
     7441 ttttccaaat tgaacgtcaa gagcgttaca atagaaggtt catcatcaac aactagtatt
     7501 tttttcattc aaatctcctt taaataggtt tcattctgtt tatattttag catagaatca
     7561 gtacaaaggg ctattttaca aaagaaataa atggtataat aggaaggcac tttgctgaat
     7621 agaaaggaag agcagtcaat gattggaatc agtgcatgtt taggcggtct ggcctgtcgc
     7681 tatgatggac agaaaaaaga agtaccagaa ttgatggaac tagttgaaac tggagaagct
     7741 gtcatggtct gtccagaagt gatcggcgga ttaccaattc ctagagatcc cgctgaaatc
     7801 attggtggaa atggattcga tgtgtggaaa gatcaagcaa aagtatggac gacaagcggc
     7861 gaagatgtaa cggaagcgta caaaaatggc gcgatcaaag cctatcaaaa attgcaggaa
     7921 aaacacattg atctagtcat catgaaggaa aaaagccctt catgcggaac ccattcgatt
     7981 tatgacggaa gtttttccgg aataaaaaaa aatggtgccg gagtggctac tgcttatttt
     8041 attcagcaaa aaatgactgt gctctctgat gaagaatggc tgaaaactag aggtgagcag
     8101 aatggaaatc gaaaagacca atcgaatgaa cgcgctcttt gaattttatt cgaccttatt
     8161 gacagaaaaa caaatgaact acatggaatt atattatgca gatgattttt cattaggtga
     8221 gatcgcggaa gaatacgaag taagccgaca agcagtatat gacaatatca agcggacaag
     8281 caagatcttg gaggattatg aaaaaaaact ccatcttttt tctaattata ttgttcgaga
     8341 acaggtattg gaaaatatga ctgcatatat tacagataag tacccggaag ataaaaaact
     8401 acttgaatat gtacagcaaa ttcaagcaat cgaagaataa ataaaaggaa tgaagaaaat
     8461 atggcatttg aaagcttaac tgaacgtctc cagcaggcga tgggaaaaat aagaaaaaaa
     8521 ggaaaagtat cagaagctga cgtaaaagaa atgatgcggg aaatccgcct tgccttacta
     8581 gaagccgatg ttaacttgca agtagtcaaa gattttacta agcgcgtacg cgaacgtgcg
     8641 gtaggggcag aagtgctgga aagtttatca cctgcacaac aaatcgtaaa aatcgttgat
     8701 gaagaattga cgatcacact tggttctgaa acagcagagt taaataaatc accaaaaata
     8761 ccaactgtta tcatgatggc tggtcttcaa ggggcaggta aaacgacctt tactggtaaa
     8821 ttagccaatt atttaaagaa aaatgaaaat gctcgtccgc tcctaatcgc aggggacgta
     8881 tatcgaccag ctgcgatcga ccaattgaaa gtattaggtc aacagctaga cgttcctgta
     8941 tttgatatgg gaactgaagt cagcccggta gagatcgtcc gtcaagggat ggagttagca
     9001 aaagaaaaga aaaacgacta tgtattgatc gatacggcag gacgtctgca tatcgatgaa
     9061 acattgatgg atgagttgaa acaaatcaaa gaattgacgc aaccaaatga gattttgctt
     9121 gttgtcgatg ccatgactgg tcaagatgcc gtcaacgtag ctgatagctt caaccagcaa
     9181 ttaggtatta caggggtcgt catcactaaa cttgatgggg atactcgagg cggggcagca
     9241 ctatcgatcc gctcagtgac aggcgcacca atcaaattca tcggttctgg tgaaaaattg
     9301 actgacttag aagtattcca cccagatcgt atggctagcc gtatccttgg tatgggggat
     9361 atgctgacat tgattgaaaa agcgcagcaa gactacgacg agaaaaaagc ggaagaactt
     9421 gcacagaaaa tgcgggaaaa ttcattcgat ttcaatgact ttattgaaca gcttgatcaa
     9481 gtgatgggta tgggcccact ggaagatcta atcaaaatga tcccaggaat gaatcaagtc
     9541 cctggaatcg aaaacgtcaa ggtagatcca aaagatgttg aacgcaaaaa agcgatggtt
     9601 tattctatga cgccagcaga aagagcaaat cctgatctgt tgaacccaag ccgtcgtcgc
     9661 cgaattgctg ccggttctgg aaacagtgtt gttgaagtga atcgaatgat caaacaattc
     9721 aaggaatcaa gaaaaatgat gcagcaaatg tccaaagggg atatgaacat cccaggtatg
     9781 gaccaaatgt ttggttcagg cgtcaaagga aaactaggca aaatggcgat gaatcgaatg
     9841 atcaagaaaa ataagaagaa aaagaaaaaa cggaaataag atgaaaaaaa cagcttcggt
     9901 acagactgaa aatgtctact gccggagctt ttctatcagc aatttttatt ctttactaat
     9961 acaattactc taaacgaaga ggtggcacag atgagatgtc cgtggagtga acaaacagaa
    10021 agtatgcggc aatatcatga tactgtctgg ggtgttcctg aatatgatga ccaaaagctg
    10081 ttccgaaaat tgatgcttga tattaatcaa gccggattga gctggcagac gattttaaat
    10141 aaaagtgaag cattcgatga agcatatgat cgattcgata tcaaaaaagt cgcaaattat
    10201 ggagaaacaa aaatcagtga actaatggaa aacaaaggaa ttattcgtaa ccgaagaaaa
    10261 atcgaagcag ccgtccaaaa cgccaaagca gtcttgaaaa ttcaagaaga aatcggcagt
    10321 ttttcagagt atctttggtc gttttctaat ggaaaaatcg ttgattcttc ttatcattca
    10381 caagaagaag tcccaacaac aagtcctctt tcagatgaat tagcaaaaga tctaaagaaa
    10441 agaggattta aatttatcgg aagcacaaca atctatgctt ttttagaagc tgtcgggatc
    10501 atcaatgatc accttgacag ttgtttccgc aaagaagagg tcgtacgtat cggcagaaag
    10561 ggcgaataaa gcatgcgaaa aatcatattt tatggtgcta taagtttaga tggctatttg
    10621 gcaacgaaaa cggatgatct tcaatggctt tttgacactc caacaggtaa gcaaactact
    10681 tatgaagctt tttatgaaac aatcgatacg accatcatgg gcagaaaaac ttatcaggaa
    10741 gcaaaaaaat atttggagac cgaagcaatt tatccagaga aaaagaattt cgttttttcc
    10801 acccacgcac aattacaatt gccagatgct gaggtcgtaa acgaagatcc tgttgctttt
    10861 ctaaaaaatc tacaaatgac agaaggaaaa gatatctggg ttgtgggtgg cggtcgattg
    10921 atcaaaccgc tattggaaca tcaaatgatt gatgaatggt atatccagat tacccctgta
    10981 ttattagggg acgggattcc tttattccaa aaaggaacat atgaagcgcg ttttgaaatg
    11041 ctagacacga cacgattcgg tgaatttatc gaattgcatt atgtacgaaa gtagaagtaa
    11101 ataaaataaa aaaggcaact atatgcagaa atgtcctgta catagttgcc ttttttatta
    11161 ccgttatgct ttttactctg ttaatttgtt agaaaaatct agatacgcat ctaaaatcgt
    11221 ttggaagaaa cttaatgttc cttcaacgat attaccattt tcatcaatta gattcgcaac
    11281 gtttccgata tatgcttccg gttgctgcaa ggttggcaca ttcaaaaaga caagtgattg
    11341 gcgtagatgg tggtttgcgc caaagccgcc gatcccacct ggtgatgcag taacgactaa
    11401 acctggttta tgatcccaaa cactgtgtcc ataaggtctt gagcctacat ctaaagcatt
    11461 tttcaatact gctggcacag aacggttgta ttcaggagta acgaaaacga caccgtctag
    11521 tcccctcact tcctcacgaa aacgagtcca ttcagcagga gcctgttctg gagtttcgag
    11581 atcctcattg taaaatggca gatcatcgat ttttacaaat acactttcgt acccttcagg
    11641 aagcaatttc gcaaaagctt ctgctacttt tttgttgtaa gaatcttttc ttaaactacc
    11701 aataaaaaat ccaatttttt ttgtcatacg atttttcctc cttcaaacta tctataataa
    11761 tcttacaaaa gataagtaac tggagcaaat aaaaacactt actgacaaaa gctacataga
    11821 agatagatta cttattttta acaaaaaaga agtctaaaat cttctatttt ctaaaaaaaa
    11881 tcgaatttaa tcaagctgta aagaaaaaaa gctttacagg gtaaaaaaaa actgttacac
    11941 tacatcttgt gaataaaaca atggaggtgg aaataaataa tggcagttaa aatccgttta
    12001 aaacgtatgg gttctaaaaa gagtccgttt taccgtatcg tcgtagctga ttcacgttct
    12061 cctcgtgatg gacgtttcat cgaaactgta ggtacttaca atcctttgaa agaccctgca
    12121 gaagtagttt taaaagaaga cttagttctt gactggttat caaaaggtgc acaaccttct
    12181 gacactgtac gtaacatcct ttcaaaagaa ggcgttatgc aaaaacacca cgaagctaaa
    12241 ttctcaaaga aataaggtga ccggatatga aagatttaag cgacttagtc ttaacaatcg
    12301 ttcgtccgct agtcacttat cctgatcaag tcaagttaga cgtcgtagag tcgaaagact
    12361 tttacgagta caatctaaca gtggctcctg aagatattgg ccgtatcatt ggaaaacaag
    12421 ggcgtgttgc taaagcaatc cgcacaatcg tttacggtgt tcggatcaat gcaccgaaaa
    12481 aagtgcgttt gaatatcatc gatgataagg aaaagtaaat aaacgtgctg tactcgaatt
    12541 ttcgagtgca gcttttttgt tttttttatt aattgaagaa atgattattt ggcaaaacaa
    12601 gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa gaaaatgtta
    12661 tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga atcagacaat
    12721 acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag tagtaccaaa
    12781 acggcttcca ccactgaaca aacaatggct acctcaaata atgagaaaaa gactgcttta
    12841 gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc agtctcttca
    12901 ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt atattacaga
    12961 acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc tattgcaacc
    13021 tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt gcagccattt
    13081 gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg gtatcagcaa
    13141 ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat tcgaatccgt
    13201 gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt agcttatttg
    13261 gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga tatgggtgat
    13321 acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta tacgatcact
    13381 catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt gtgaaatcag
    13441 cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa aaatagcacg
    13501 ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt gcctcttcac
    13561 acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg aatacttgaa
    13621 tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta tctcaacaac
    13681 ggatttccca gaagaacgtt acaaaaaagg aacggtgcta acactattcc aagacggaaa
    13741 agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg acttattgag
    13801 ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca tattaaaagt
    13861 gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg agatcatcgg
    13921 tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct tgtcaccagg
    13981 tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc ttccatatat
    14041 cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag aaattccaga
    14101 aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg tttgaaggtc
    14161 ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat atcaatatct
    14221 ctaacttccg cgattattca gataacaaac atcagacagt agatgattat ccatacggcg
    14281 gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa gccattgaaa
    14341 aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt aaacgattcg
    14401 atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt tgtggtcatt
    14461 atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc ttaggagatt
    14521 atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca gttcggcttt
    14581 tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca accggtttat
    14641 tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta ccagaagtat
    14701 tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca ctaagaagga
    14761 cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa atgacgaaat
    14821 ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc aagtgacttt
    14881 ttctaaaatc ttctttacag taggttgata atatgttagt attttatggt gagtgcaaaa
    14941 gcacttaact attacgatat tccgctgtga gaaaagctca taagaatatt tggaataagg
    15001 agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta cgttctgaca
    15061 tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt gaaggtactc
    15121 gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct ggaatcagcg
    15181 aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca ttcccattgc
    15241 atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt cgtgcaaaat
    15301 tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc cgtcgttaat
    15361 catcgttatc agacagagaa agaacccttg gtattcaagg gttctttttt tattatctgt
    15421 ttttttatat taacgatttc tatttggtac atttttgatt aataaaaagg ggcaaaaaag
    15481 gggcaacaag ttacagtttt tctatatctt ctttcaattt ttcttttact ccttttgtca
    15541 catgcaaata aattttttca gtgatatcac tattttcgtg accaacgcga tcctgaatgg
    15601 catacagagg agttcctagt tccgctaatt ttgaaatatg ggtatgtcta aaaatatgtg
    15661 aactaagttt tttatcaatc ttcatgtcag ctttatgact tctcaaatag gtgttaattg
    15721 ctgttagttg gaaaggagtt cctttcgttg tttgaaatag aaattgacca tttggattta
    15781 attctagaag ctcattgtaa atagctatcg ctttttttgg taaatcaatt tctcgcattc
    15841 cggcagctgt ttttgtagaa tcagattttt tcatatcagc tattgaacgt tctctataca
    15901 tcatcgtccc atttataact actgatgcat tattattggt aatgtgtaca tcatctttac
    15961 ttaatgcgat tgcttcacca gacctcatcc cagtcaaata tagccactgg aaaaggagag
    16021 aatatctttt attgtgtgaa gttgtgaaat ctactagtct attatattca tcatcttcta
    16081 aaaatttatc tttaattttt atcgtttttg actctctttt ataatcaata actacttcat
    16141 cgataggatt tttttctaca taaccttttt tcatagcata tgaaaaaaga agattcaatt
    16201 tggatttaat tacactgacg tacttatttg acaagtcatc tttatatatc atgtcttcaa
    16261 aaatattatt caaatctatt gtattaatac cagaaacgat gtaagtctca ggtatcttct
    16321 tctttatagt gtttaatata ttattcgtag gataatatgt agattcctta acttgtcttt
    16381 tgtatataac taaccattct tcaacaagct catgaaatgt tagatcaggc ttatgaagtg
    16441 ttttttcgtt taattttata tcaatttttt tatttaactc tagcaatgcc actttctgag
    16501 tttctcgaga tttattttta taagtaatgc tcactttttt tctttttcta gtttttggat
    16561 caatatatcg ctcattatat ttatatactt tttttccgtt tttatcagtt ttagtttcga
    16621 tccacattgt tttcatctcc tatttgttgc tataatagga atagataagt aagccgatta
    16681 tagcaggttt atttttcatc acgtccacaa actttggcga gggaggggcg tgtttttgtt
    16741 agttattgtg gtaggttatc taaagcgtat tgtgcttgct catttgtaaa accttcaaat
    16801 ataagttgat cgtacaattg cgaatctgac attgatgccc aatcatcgta actttccgct
    16861 ttagccaaag cttgttcgtt gtaatcgaca tcaacattag ccagcgcata atcgattgca
    16921 tcacttggat attcttcaaa ctctagttgt tcacgcaaac cttgttcaga cataccagcc
    16981 caacctagat agctttcagc tttgctcaat gcatttctgt attcacgagg aacactttct
    17041 tcttgagtaa cttcttctat agtagtttca gtagtttcag tttcttcttc aacgactgta
    17101 gattctgcta ccgattctgc agaagacact tctgtcacgc tttctttagt tgtttcagcg
    17161 acggtatttt tagcatcatc actgcctcct tgcgaaccta tagcaataaa caccacaaca
    17221 accgctaaca accaaaacca tacccgttta taaaacggtt tcttaacttt atacattttc
    17281 ccatcttgac ccataacttt ttttgccatt taaatatccc tcgcttcttg ttataatata
    17341 tttgtgatct cagaaatgag gtatgagtcc gtgttgcagc acgggctttt ttctttataa
    17401 cttttttaga gattataggc aaaatagtag ggcataaaaa tgtattattg aattccgtat
    17461 ttagaaaatc ctaattgaac ttcaccggaa gtcttttgct gtgttgtacg caatgcttct
    17521 tcttcagaca ttccattctg tactttccat gcaacaggcg acatcccgta tttgttaaca
    17581 aaatcagtaa gtgataaagt gtcagcgtct tgctgagcgc ttgtttgttg gtcttctgga
    17641 ttctgttgag atgctgcttg ttgttctttc tgatcttgac tgataatatt gccagcatca
    17701 tctgtagtca atccattttc ataaagggcc acgccgaaag cttcccactc tttgttggac
    17761 caatttgcac gatcagctgg agttgactgt aaagtgcgtt gtttcatctg ttcatatgtt
    17821 tcttcttgag gtgcggtttg gattgtactc tgactggagc ttataactgt tgggctaggt
    17881 tccgccgtag cttggttgga gcttgtaact gttgaactag aatctgtttt agatgtagat
    17941 ttgctagtag atgaactggt ttcagttgtt tcttttgttt tacttatttt tgtttcttgg
    18001 ttagaagtgg tatctgttga ttcagctttt ttattatttg aacaagctga aagtagcaga
    18061 gcagtactta acaacaacat aacgctaact tttttcattt tataattcct ctttctcgtt
    18121 gtaatatgtg tgctaacacg ggctttttta tataagaaaa cgataagcgc tttctggaag
    18181 cccgtaaaga ttctttaatt cctcgatttt tttaggatat tgatcattat cttctttata
    18241 aagagaaaca atgagattag cagcaaagca attagcttcg ctttcagatt tgcttctaga
    18301 tgttcttgtt gatacataat agctggataa gccacgatga aaaatagcgt gacctaattc
    18361 gtgagcgcaa atgtagaatc tttcctcaga gtctcgcagt tcatcattta aaaagattat
    18421 cgcacgatct ctaatttctt gaaactgccc cttgggattc tcgataaaag gaacgtattg
    18481 aattttaatg cccatctttt cacaaatata aaaaggatta gcggactggt atttccgctt
    18541 caactcctcg actaaattaa tcgtatccat ctccataagc tcacatcttt ttgccttttt
    18601 ctttgtcttc tttcacaata tcccagaaag tcgctatcag gatatctttt acgcgctgta
    18661 tttgttcggg tgtcaatgtt tccccaccat aagacatatt aacatttgag tctagtagtt
    18721 tatcaagttc aaccacttcc tcttttgtag cccacttggg aacattatta tttcccaata
    18781 aataatcagt tgtgacacca aaataatcag caactttctt taagttctca gattttggcg
    18841 aggctttatc ccatcttctt atttgtccat tagagatgcc cacctgtctt tctacttctg
    18901 ctatagtcac atgcttttcg tctgctaatt ctttaatctt agtaactaaa ctcattatta
    18961 tcaacctttc aaagctgaaa agaaaataaa tagcttaaaa gttatatttt tggttgacaa
    19021 ttagctttta agctagtata tttaattcgt aagctaaatt gttagctaaa taagagcaac
    19081 aaaaaactct actaatttaa aacattctct cggtcgccaa acttagaaat gttattttag
    19141 aggcttttta taagtcttat ttaactatgt attcataata gcttaaaagc taataggtgt
    19201 caacgattta gctaattttt tagcttacaa attatttgtt tagaaaggag ctatttttat
    19261 gtctgagaat ttagacttaa aaattcgagc ggagatgaga aaaagaagaa tgactttcaa
    19321 agaactagct gcgcttatcg gtatttcagg agcttattta tcagatattc taaacggcaa
    19381 tcgtgatgga aagaaagcac aacagcatat cgaaacagtg aaaaaaatat tggacatccg
    19441 ataggggggg taggcgatga caaaactaaa aaaacaagat tttgtaaaaa aatataatta
    19501 ttctccatct acttatcaac gtcgaatgtc ggaactaaaa aatacagcaa ttttctcagc
    19561 ggcgtatgaa cgggtcacag gacaagaagt ttggatcaat acagaattat acgataaatt
    19621 tttgtctttc aaatcctata acaggttacg cacaagaaag gtaacaccta aagaatttat
    19681 cgagaagcat ttagttgatt tataaaaaac agaacatttt gagaggtgaa ggttaatggg
    19741 taaattcaac agagcattag tattcagcgc accgctaatc gtctatgctt tagggctttg
    19801 gggaagtaga caagcgttga taggaacgat tgtgtacatg gtttggattt ttatagggct
    19861 ggatgaagct gagtacaaaa caaaaaagcc agtcgggagg gactgactaa tgagtaagga
    19921 cgatattgta tttctgttaa ttatgttttg tgggattgga tcaataataa ttaatttgta
    19981 tttgattatt aaagaaaaat ttttcaatta aaatgagtct tttagcttta acttaaattt
    20041 tttttgataa ttagctgttc ttatgaccat tacaagtttt tcaccaggta atatatcttt
    20101 catatctaaa acttctgaaa aagcgactga gtagccagga gagattgctt gtgccaacgg
    20161 tataggagat aaaaggctac ttttatgaat gcttcccata tcaaaaccat tggcgtgata
    20221 atgtgaacaa ttaattttct taccgttgta aagatacgta cattcgataa ttgagtttgg
    20281 catttttgat gggtttgcaa ttacgccagt aagaatcatt cgattacttc tatcatgctt
    20341 aactaaaaaa taggagagcg taatttttct tttatcttta aataagttat agagggataa
    20401 aaaaaatcct gaaatagaaa tgattaaagt aatagcgact tggtaatcgc tcaaaatttt
    20461 tatcaagtaa ttcaccacct ttttactcaa attatatcaa aaaggagaga agaaataatg
    20521 caagaattag taattttgaa aaataaaaaa agacacgaac gccggcaagc atgatcgtgt
    20581 cagggaaata aactatcttt ctatatttta ccacagaaag gaatgaaccg taaatgctaa
    20641 ttgcaacgga tacactagac cgcatctttt taaaagacga atacaaactg cgcaatatag
    20701 atgcgtcagg aattttagta tttgatcttt atgacaatgg aaaaattggt atctatcaag
    20761 caagtgatat tgaagaaaca aaccttgctt tcgaacaaat tgatgattct gtggaattgg
    20821 atttagatga ggcaatccta gcttttgaac aaattgcaaa attattaaag gaggcacaaa
    20881 agaatggcaa ctctttacca actcagcgag tcatatatca aagtcctgga actggcagaa
    20941 caattggatg aagaaattct tcgcgatact ctcgattcga ttaatgaagc gatcgagtat
    21001 aaggcagaaa acttagcaaa aatagttaaa gaagtagaag ggaaagctga gttaatcgat
    21061 agtgaaatta aacgtctgca ggaacgtaag acatcgcttt tgaacaatgc taagagtatc
    21121 aagcactatt tacaagagga aatggaaaag actggtaaaa cgaagattaa aggtgaatta
    21181 ttcaacattg gaattcaaaa taatccagta tcggtcaatg taatcaatga gaacttaatt
    21241 ccaaaaggat tctttacccc tgtgcctccc aaattggata aaaagcaatt gaaggaggaa
    21301 ctgaagcacg gagatattcc tggtgctgaa ctcgtccaaa caaaaggttt gaggataaga
    21361 tgatgaagga ggagaaataa tggaacacga aaacgtaaaa gacgctttga aagctgctat
    21421 cgaaatcgcg gaagctaaag gaataaaggt tgacggcaaa ccagcaactg tccatgacat
    21481 tcaaaatttg acgaaagaac atctttattt tatcgccgac ttgcttggtt tgtcggaatt
    21541 gtatttagac aaataacccc acacggaagg agtggctagg gtggttgtat tacaagtaat
    21601 agtctgcgta acattcattt ggtcgtgcat accggtttgt atatatatca tgaagaagct
    21661 tcgccctgag tcttacaaat tatacgcaat ttacagcata acagtagctc ttgcatttac
    21721 attgtttgca atttctaggc ttccgtttta atcgtgggaa tgaattttta atgaactaaa
    21781 ttatctataa agcaaaaaaa ggagaaacaa cattgaaaaa caaactagca aaaacaacag
    21841 caatcatcgg actagcacta ggtagcggcg ttatcggcta cgcagcaagc aatgcgtttc
    21901 aagatttgga cacgatcaaa gcaaatttca acacagtcct acaatacggt caaacgaaat
    21961 cgcaacgtgt gtcagaactt gaatcacagc tatccaacaa cactcgcaca caggagcagc
    22021 tgaaagccga aattgagcaa atcaaatcgg acaagcagaa ggaaattgaa gccaagcaac
    22081 gagaagtcga acaaaagcaa caggagatcg ctacaaagca acaggaagcc gatagcttgc
    22141 gccaacaact atccacagtg caaaacgaca aggagcagtt agaacagcgc gtgagcgagt
    22201 tacggcagta tacggatcaa aaggtagggg agttggggaa atgaaagagt tgactattta
    22261 tgacccaaat atttcatggg ctaaacacac aattaaagtt tcttttatga tttggggtta
    22321 caaaggatat gttacttaca aagtgggcgg taacactaag ggtttatcat taatagctat
    22381 tgattcagat gatctatatg atgctaattt cgaggataac ccagttaatt tccgtgacct
    22441 tgatgaagat tggttctcta tggaacttac taacgataaa ggcgacagta cacttgtaga
    22501 agatgaattc gacagactag gtgattatat tgtcggtgtt gaaattattg cgcacgaacc
    22561 agagtaggag agtgaataca aaatgattaa aagaatcact atatcagttg aagaagatag
    22621 tggatcaaca atgcagcata ccgacaatcg aaaaactttt ttaaccaata gaaaggagaa
    22681 ttttgatgca gattaagaag gcgacggata tcgaacaaac caagggaaca tatttgatct
    22741 acggtgctcc aggaaaaggt aaaacttcaa cggctaaata ttttccaggg aaaacactga
    22801 tccttgatat tgatcggact tcaaaagtcc ttagaggtga gaaaaatgtc gatatcgtat
    22861 atatcgacaa cgaagatacc tggaacgact ggggcaacac gttggctgat ttaactacca
    22921 attatgtagg tgtttatgac aacgtggttg tcgataatgt cagcgaattg gaacggtgta
    22981 ttctgtccag tttaggcgca gaaggaaaaa acaacggcgt tccttcgcaa ggcgattatc
    23041 aatatatgca atttagaatg gtgaacagcc tacgctatat gaaaaattta gacagcaacc
    23101 ttatctggac cgcatgggaa gaaatcgatt tgtggacgga ttcggatgga tcttcttaca
    23161 acatcgcttt gccgcagatc aatcgaaaga ttcgaaacaa tattttggga ttatgtgacg
    23221 ttgttggtcg attgatggta aaggaagacg atgaacgcgg atttatttta caagccacag
    23281 attcaaccta tgccaaaaat caattggata atagatctgg ctgtaagcaa agtgagctga
    23341 ttttagatgg aattgtacga ctaccagaat gatttagtcg atcgtgcaag acaagcctat
    23401 gttgatggtt acaaggcacc atgtatcgta tcaccgtgcg gttctggaaa atcagtcatg
    23461 gttgcagaga tcgctaaacg aaccacacga aaaggcaaca gagtgttgtt cttggtccat
    23521 cgtcgtgaat tgattgatca gatcaaagat acgtttcaaa agatgggcgt agataatcag
    23581 cttgtaaatt tcggcatggt tcaaacagtc gtacgacatc ttaaaaccat caagaaacca
    23641 caactgatca tcactgacga aaaccatcac gggttagcag cgtcctacag aaaaatctat
    23701 gagtattatt cggacgttcc gcgattaggt tttaccgcga cacccatccg gttaaacggt
    23761 agtgggttgg gggatgtgaa cgacttgcta attgaaggag tatcggctaa atggttgatt
    23821 gagaatcata gattagctcc ttatgaatat tatgcaccta aattgatcga tacagccgaa
    23881 ttaaagaaag cttcgactgg agattttaca aagaaatcaa tggataaggc agtgaagaat
    23941 actatttatg gcgacgtgtt gaaacattat aaaacgttag cagaaggtga acaggcaata
    24001 gcatactgtc acagtatcga tgcaagtaaa cacactgctg aaatattcaa cgatgctggt
    24061 tataaagcaa cacatatcga tgcaaaaact ccaaaagatg aacgtgcaga tattatcgaa
    24121 gcgttcagaa atcatgaaat taaaatccta tgcaatgttg atttgatcgg agaagggttt
    24181 gatgttcctg attgttcgac tgtaattatg ttgagaccta ctcaatcgtt atcattgtac
    24241 attcagcaat cgatgagggg gatgcgttac cgaccagata aaacgtcaat catcattgac
    24301 catgtgggta atgtgaaccg ttttggattg cctgatatgg atcgtgaatg gagtttagat
    24361 acaaaaaaga aaacaaaaac tgatagtgat ttatctatcg ttcaatgcgc gttttgtttt
    24421 ggtgcttacg aacggccaaa aggcgataat atttgtccgt attgcggaga gttgcagccg
    24481 attgaagaac gaaagtcaga gttagaaatc gatgaatcgg ctgaattaat gaaagtcgga
    24541 gaaacgaaaa ttactttgaa ttttgagaac aataaatatt acaacatgac ggaagatgaa
    24601 gcagaaagta tagaggactt atatgccata gcaagagcta aaggatttaa acctggttgg
    24661 gcttatatgg ctgctaagag gaaggggtgg ttgtaggaat gggcggtatt tttggtaaaa
    24721 aagcagaaga tgttcaaggt tcaataataa atggcgtaac aataataggt gatactggaa
    24781 aaagagatag ttcaggcaat caaattgtaa tcgctagaaa taaaaataac gaagtttttg
    24841 aatcttctct atctaatata aaaagaggtg ttgttaacgg gacaggcgaa gagagtggct
    24901 acgttaattt aaaaaaagct aatgaaaaat accatgttga aggaacttac gttaaggctt
    24961 tgtctgtcgg tggaacaaaa ggcgttaatt gggataaacg aagaaataaa tggaaagcac
    25021 gattatattt caaaggagaa tatgtcttac aaaaggattt caacaccaaa gaagaagcaa
    25081 tccaagcacg acttgcagca gaagaaaaat atttcaaacc tatcttagaa aaatatgaaa
    25141 ataaaaagga gaatgattaa catgacagga tttaacttag acttttcaaa agcgcaacaa
    25201 gggaacgaaa tcaaagacgg tacgtacgaa gtggtcgtaa acaaagctgt agaaaatgca
    25261 actaaatcag gcgcagaatt tatcgatatc gatttgatcg ttcgtaacga tgtggatcaa
    25321 ccgttccaaa ataaacacat tttcgcaaaa atctggaaag ccaaagctac cggcaaatat
    25381 aacgaaggta tgatcatggc aattgcacaa gcgctacagt tggaagacgg aaaatcatat
    25441 aacgggtttg atgaattgct ggcggacttt gttttgaaaa ccgcctcagt acgcgtgaaa
    25501 actgaagagt caaatggata taaaaacgtc aatgtgaagt cttgggataa aacgaacaca
    25561 cggggcgtaa tgaatcacca attcaaaaat ggtgatgaac catccttcgg accggaacgt
    25621 tcaagagcga caacggttag aaatgacaat cttccatttt aattgaggtg taatcaatgt
    25681 atgaaaaaat tccaaaagag cttaaaaatc tcaaacaatg gtgtgtttat aagctagttt
    25741 gggatgaaaa gcggcagaag cacacgaaga tcccttataa cgccaacaac gggcataaag
    25801 ccaaatccaa tgacgagagt acctggtctg attttcagac cgctctcgct gctattgatc
    25861 actacggaat gagtggactg gggttctttt tcaaaccacc ttatttcggc attgatatcg
    25921 ataatgctga aggtgaagtt gagcgattta aatcaggtga tatagaagaa aatattatct
    25981 atgaatttat cgaaagcatg agatcctatg ctgagtattc tcaatcaggt actggcatcc
    26041 acattattgc tcgcggagaa ttgcccggcg gaagacgcag gaaaggtgac gtagaaatgt
    26101 accagaacgg ccgattcttc gttatgactg gaaatgccgc atcaaaatat ttagaaataa
    26161 ctgagccgaa tccaaaggat atcaaacgcc tatatgatcg ctacgttgga gatgaaaaaa
    26221 tcatccaatt caaagaagaa aatccgctga tgaataccgt tgatttaccg gttgaagaaa
    26281 ttatcaaacg tgctgaatcg tcttcccaag gggcaagatt caaagtcttt atgaacggcg
    26341 gttgggaagc tttttattcc tcgcaatccg aagcggatat ggcgttcgcc aacgacttgg
    26401 ctttttggac cggtagagac tttgaaaaaa tggatgagat tttcagagcg tctgcaatga
    26461 tccgtagcaa atatgaccaa aaaaggggat cggcaactta cggagaatct ctactaaata
    26521 aggcaattgc tgatacaaat tccgtctaca accccaaacg gaaatctgat tttaagattt
    26581 ttatcaaaga ccaggaacag ccaaaagagg aaaaatacta ttcgtacgac gacaccggca
    26641 acgcggatcg gtttactgat atctacggaa cgttagtcaa atattcgtac attgataaat
    26701 cttggtatta ctacgatggg aaagtttggc tacaggacaa taccggcgaa gttcggaaaa
    26761 tgatcgatac gacagtagac atcatgggaa aagaaccgtt aacgatccct gaaggcgcag
    26821 acgatgaaac caaagaagct ttgatgaaag cgaaagaaaa acacgtcaag cgttctcgca
    26881 gcaatgccgg aaaaaacgct atgatggacg aattgaaaca tcgactatcc gtattgccgg
    26941 aagagttcga caaagataaa acgctgttca acacacaaag cggctatctg tctttgcatg
    27001 acggtcttct acacgagcat gagatagaca aaatgtttac acgtgtttca aatgtcgagt
    27061 acacagaatc ggtcgattgt cctatgtggg aagaatttat taatcagatt tttgacaatg
    27121 atcaagaatt aatcagatat atccagaaat gtgtcggata ttccctgacc ggatctacca
    27181 gagagcaatg tatgttcatc ctctacggtc atgggtcaaa tggaaagtcg gtatttttgg
    27241 aaattatttc tgaattgatg ggaaactatg cgatgactat gcaggcacaa acgatcatgg
    27301 tcaagcaaag ccaatcatca gcaaacagtg atattgcacg attgaaaggt gcgcggttgg
    27361 tcacttcgtc agaaccgaat gaaggcgtcc gactggacga aggtctggtc aaacaattga
    27421 ctggtggtga taaagtcacg gctcggcacc tttatggaaa agaattcgag ttcgaacctg
    27481 aattcaagtt gtggctggca acgaaccaca agcccatcat ccgtgggacc gatgacggca
    27541 tctggcgacg gttaaattta attccattca ctgtccaaat cccggatcac aaaaaggata
    27601 aaaatctgaa atttaaattg cagacggagt tgcaagggat ccttaaatgg gcaatcgatg
    27661 gttgcctgtt gtggcagaga gagggcttgg agaaaccaaa gtcggttgtt gcagcgagtc
    27721 aggattatcg aaacgaaatg gatcaaatag gtacatttat cgaaacgtgt tgcgaaacag
    27781 gtccaggact aaaaatatca ggcggagaat tatacaaagt ttatcgtgaa tgggcatcag
    27841 ataatggcga gcatactttc actaacacga agtttggcag agagatttca aagaaatata
    27901 gcaaagaaaa agctggtgga tttatggttt ataaaggaat cacattaaaa ccaagaaaat
    27961 acgataacgt tagagaactt tttaaataaa tctggagggt tctggagggt tgagaacgaa
    28021 accctccaac ccttagaaac gttgatatat cagtatttat attatatttt tctttctttc
    28081 tggagggttt taaaaaaata ataaagtatt aaagtaaaaa ataaaaagta aattatataa
    28141 aaaagaatag gtttttgggt ctgaccctcc aaactctcca ccaaaatctt cctaaatgct
    28201 ttgataccaa cgattaaaca gtctggaggg ttacattgaa atcagaacat caaatacaag
    28261 acgagatcag aattgcctta tcaaaacatg gttgcacgat ttttcgatcc aatgcgggac
    28321 aagtgacaac gatcgaaggg cggcaatttt acgggatgcc aaaagggttc ccagatctat
    28381 gtggacatcg taattctgat gggaaagcaa tttatataga agtaaaaaat gaacgcggta
    28441 agttgcgtga agatcaaaaa aggtttgctg aatttttaaa cggtcaacct gtactatacg
    28501 gtgttgcacg atcagtggaa gaagcattga aaattgtgga ggaatcatca tgacaacaga
    28561 agaagtgatt caaatgcgta ttcgaagcct tcagcgtgag attgacgatc tggaacggac
    28621 aaaggcagtg atggtcaatg aaacggcgag gaaggcaatc gatttgcaca tagagaattt
    28681 aagaagggaa atccatcgat tggaggaatg agcgtggata agaaagcagc aatgaaacga
    28741 atcatcgaac tgacacattc tgagaattgg caagaagaca aagaaatagt tgcagaagtc
    28801 caaaagctcg gtaaatcaat gtggagtgaa aaaaccaaac ggagaacacc gagaaaaatt
    28861 gcaatctggc atggtgaccg aattctagta acaggtactg ctgaacagtt atctgaaatt
    28921 actggattaa gcaaaaacat tatctgggat agagctaaac gcgagaacgt tgattcgaaa
    28981 ggtcgtcaat ttaaacattg ggagaagaaa taatggagga actaatcaca aaagtagagc
    29041 agtgggcaaa agataaggga ttggatcaag ctgattccag caagcaaatg ttaaaaacga
    29101 tcgaagagat tggggaagtt gccgcttctc tagctagaaa agatgaatat ggtttaagag
    29161 acggaattgg agacgtagta gtaaccttga ttattttagc tatgcaaaat gatatggatt
    29221 tgtacgagtg tctgaaccaa gcatacaacg aaatcaaagg acgcacagga aaaatggtag
    29281 atggtgtatt cgtgaagtcg agtgatttgg aggacagcga tgaataaaaa agtattaatt
    29341 gataaacaag cattgattga tgaattaatg aaaatacctg gtgtgggatc taatagtgac
    29401 gctttagaaa cgattaaacg tttcccatcg tatgaaccgc agaagccagt tgtgcctaag
    29461 tttgtggcgg aactactcga ctattatcgt cagtcaacag atgttgattt attagcttta
    29521 ttgataactt tccatgattg gtattatcgc aaaactaaag acggtgagca tgaagaagca
    29581 attgattggc tagttgatca tcctgaaatt tatatgcgtg catggctgtt tggctacgag
    29641 ctcgagaaag agccgttgta ttgcgtaata atcaatggta gatatcttgt caaagtattc
    29701 agcaatacaa acgttgtcat attagttcca gcagatgaat tttcacagta cgttactcaa
    29761 aaatttcaat taactgaaaa acaaatcaaa gcaattgatg aaagatactg gccgtttgct
    29821 gtgccagtgg atgaggtggt agaaggatga gcgacccata cattactaaa ttagaaaagt
    29881 tgtccgaaga ttttgagaag aataaaaata aactaactaa agataaactt atagaactgg
    29941 ttaggttggt tatcagagga gctattacaa gagaaaaggc gcagcaaaac atagaaattt
    30001 ttgctcgtag tgtccgagat ggattagaga agaaagttaa acaaaatgaa caacagacac
    30061 cgcagaataa caaaactaag aaaacgggaa ctgaatgtac taaagacaaa gtttgaaaaa
    30121 gaatatggaa tttcagcaga agaaacatat aaagtggcaa gtcagtgtgt tgctgatgcg
    30181 agcggcgcta ttcgtaagtt tggaatttcg atattaagtg atgatcgtaa atgggaggaa
    30241 aaagaatgaa actaaaagac ggattttact ccagcagtca tggtatcggc ggtttaatgc
    30301 tagatatgcc gacaaagaac cctaaaacac gtgagaaacc aaaattcaaa gtcggtgaca
    30361 tggttcgctg tgaagcagaa ggattcatct atccattttg tggatatgta gaacatctct
    30421 ataatcactc agcaatcatt cgtattgaaa acacgatgga atgtgacaag tggttagcga
    30481 aaagcaaaga gaatttagca gtagcgagat tggtggatat ggaggttata taaacagcga
    30541 aaaaatgtac ttctaagaga aaaatttatt ttttataaaa ataggaaagt atcattatga
    30601 aaaagaccac agagataaat aaacctacca cataaaaaat gaatatttct tgcgattcaa
    30661 tataggtata tttaacaatt tgtacaataa aaaatactat aggtaaaaaa gataacatga
    30721 ggagcttatt tgttctagag tgtttgacaa agtaacaaaa tgcgacgata ccaatacaaa
    30781 cgggaatcca aaataacagc atgctccaca tagtcactca accatctcct atacgcttta
    30841 ttatcaatag ataatatcat ttttcgtaat aaagataaaa aaataagtaa ggcttctcat
    30901 taaaatataa aaaagacagc cgaccactga ctgcctatat aagagtattg aaataaaaag
    30961 ctgctgatat aataaattcc acaagtttat tatatcacat aaaggagcgg tttgacttga
    31021 tgcaattgtt acgagaggta gatttcaaac agacaagatg taatgcgaga gatgtgctga
    31081 agaactttcg gcgtttggag cggatggcag gtcgctcttt gatagatatt aagtcgccga
    31141 ttattacgga tatgccgaag gcgccgaagc acggcaataa ggcagaagac gcaatcattc
    31201 agatgatgga tatagaagcg gagagagacg cgattctagc agccttgatg gctcttagtc
    31261 tgattagtcg tcagatactc tactatagct tttgtgtgcc agatagcttc tcaaactaca
    31321 gaattagccg tgaagtgggt tattcagaaa gaagtataca acggatgaag tcggaagctc
    31381 taatagagtt tgcagaagca tataaacacg gaagaataat tgcttataaa taatttggcg
    31441 gctttttggc ggaatgatgg cggtttttag ctatttacca gtgatattat ggtagtgtcg
    31501 aaagattagt gataggtcta agacaaaata ataataaaag gaacatcgtt ttattattgt
    31561 ttcacaatta agcttcgata gacagcaacg gaaatattaa gaataaggat gtgaatttta
    31621 actccttcta aattgttctt attatctatc atccgttgct gtctattgtt tttttaatta
    31681 ttcacacgat aactaaaggt gagcgaagag aaatgattcc attaataatt tcaatttttg
    31741 cgctttgtct taatgtctat atgattggat ttaaaaatgg gcaaaataaa agatagtagt
    31801 aactaagaaa gaaattttta tattgtcact gtggcggaaa gggtagacgc taagcatgtg
    31861 tgctaggtca atgcttcggc aactatgcaa tgttcgattc attgccagtg actttggcaa
    31921 ccgaggcatc ggcggtttaa aaatataggg gtgcgcaatt tcgtacgcgt tttgtgcatc
    31981 gtgtaggttg ctattacata ttagatcact cgttgagtgg tctttttatt ttacataaag
    32041 gaggtaacaa cgatgtatag accacaatac ttagaacaga agtatgaagt aatcactgtg
    32101 caaaacggta acggtgagat agtacgaaag tatagaagac caataaagag cgatacatat
    32161 aaacgaaagg aaaacaatga agttatttct tttcgcagaa ggagaaaagc caaatgagaa
    32221 actactggta tgtatcgcta actaatgaat atcctcgaac cattgatgat tgttcagtgc
    32281 gtgttgtgcg ttctgtacaa atcaaaggga agtactctat tgtcgaaatg ctaagagaag
    32341 ctacaccaaa cgaagtggat aaatgcaagc tgatatattg cggtcatggc tattggaaag
    32401 acgagtatat ccaacaaaac attgagaggt ggatagatag atgagttacc tcgaacattt
    32461 gaaacgttgc tacatgcatt ctaagaataa acttcctgac agctacactg tagatgatgt
    32521 tgctatacat gtattgaaga ctgagagcca tagcagtcct gatggtagca gtaaggagca
    32581 gacgctcgcg tggttcaagt tctttaagtg gattaaggag gaagagtaat gcacatgcac
    32641 ataggagaaa agatatataa attgagacgg cttaataacg aaacaatgaa cgacttagtt
    32701 aaacaaactg gagtgtcaaa atcatggtta tctgatattg agtctggaaa aaagcaacgc
    32761 gttgatttta ataagatatg tgcaatagcc aaacattata aagttagctt agaatacttt
    32821 cgggatgatt atttggatag tgatgaacac gaggatatta ttcctttgac aggcgaaaca
    32881 agagttaaat cagtgaaatc gcttgaacaa agcctaaagt atctgaataa caaacaagaa
    32941 cctaaccttc tagagatcaa actaaaagat actgactcag taccagaggt ttggtacaag
    33001 ggtgagaggt tggatgaatt gcctaaagga ttagtagatg tctcgtatca ttggaagact
    33061 gatgatttta ctaatgatga tagaggagcg aacgacatca cgattcaata cttttctagc
    33121 tttaatgata agtatccaga taagaaaaca atcggacaca agagagatat gtaaatgaaa
    33181 gaagttagac ctagagacga aatagacaaa ctatacaaga ccaaacgatg gcgagaccta
    33241 aggcaagtag taatagctag ggacttcggc atgtgccaag agtgcaagcg tcgagggcgg
    33301 aacacaaggg gcacgatcat ccatcacata gtcgaggcga gggaagacct gtcactgttc
    33361 tggtccgtag ataaccttga atgtatctgt gtagcttgtc acaacagaga gcatccagag
    33421 agatcaggcg ggaagaagaa accaaaacct aaatcacata tcgttaaaat gtattcaact
    33481 cctgaaagat aagtttgcag cgaaatgaag gtagcccccc tactctaaaa gattaaagag
    33541 taaggcttga gaagaacggt gctgtccttc cttcgtaaaa agaccgcttt tcaagttttt
    33601 tggagaaaaa ggaaaaagcc gaccaattta agccggcttt ggacgaagct atttcttagt
    33661 ccatttgttt cctttttgag aagtaggggg taatcggtcg cctggatcaa tttttacttc
    33721 ttgtccgcct tggacatttc caccacgagg acccacttct ttgtaggttc cttttggttt
    33781 gttgtcttcg ccgggtttat agagttctcc cataggaatc cctccttaac aaatttcggc
    33841 acagcactgc cgataaccta attataagga ttgtgataac gatttcaatc tatcttttga
    33901 aagaaggtga tattatgccg caaccagcga agagtgcaaa attacaatta ttaaacggaa
    33961 acccaaataa gaagaatacc gaagaactcc gcaagcgagc ggccgcagaa gacaaattaa
    34021 aaatggctac tgacaaaatc aaaccgccgt tatggctaga ttcgctagga aaggatacct
    34081 ttgagtttat cgccgatgaa ttgctgtctg tggatttaat cagtaatccg gacgtccata
    34141 caatggctct atactccaat tggtattcgc aatacgtttc tttagaaaaa cagcttcgaa
    34201 aactacaacg agagtacaag ttgaactatg cgcttgcgaa aaaggaggca gaggcgagag
    34261 gtgagccgtt taatgaacct aatgaattaa ttggtaatcc gctctctcgg cagatggata
    34321 cagcgtctcg gaatctccgt tcttttggcg ctgatttagg actatcacca gcagccagag
    34381 ctaagttagc tattaagatg gctgatgatg gtggtgatga cgatgacgac ttctaatatt
    34441 ttggatatgt cttacacaga acgtgtggac tattggcaaa gctatcttga ggagcaagct
    34501 tcttggggtg gctttttaaa atgtccatat ccggaattgt taactacttg gtatgcggaa
    34561 cgattaatcg atggaagcat accagccagc aaagaaaata ttcaagctgc taaacggcat
    34621 atgcgtgatt tgcagcgcca aggaacagat gattttcctt ggatctttga cgaagaaaaa
    34681 ggtcaccggc ctattagata tatcgaaaaa aaatgtaaac caactgaagg cgactttggt
    34741 tcgtttgttt tgcaaccttg gcagcatttc ataattggat ccatgtacgg atgggtacat
    34801 cgtgatacag gagagcgtcg cttccgcgag gctcttattt ttgttggacg taaaaacggt
    34861 aagttttgcc gtttaaaaat cgggcaaaat cggtgaaaac ctttattttt ggctttcttt
    34921 tagtaataat tcgatagctt tatcaagcaa tttagacata ggaaccatag tttcttccga
    34981 catttttttt aatccttcgt ataaatcttt atcaattgcg ttagaaattc taattctgtt
    35041 ttttagtccg tatttgtcca tgtgtgacac tcctttttat tttactataa catccgattc
    35101 caccgcttgc aactaccatc aattgatggt agtatataaa agaggtgagt agattgaaaa
    35161 aatacattat ttacaaacac ttaaataaaa taaatggcaa aatttatatc ggtgtcacaa
    35221 atgatattgg cagaaggtgg aggagcggtg ggattgaata taaaccacca aagaacgaaa
    35281 cccaacacca cagaagtttt tggaatgcaa tacaaaaata cgggtgggaa aattttgatc
    35341 atttgattat tgaagaagac ttaacgatga aagaagcttt tgagaaagaa aagttttata
    35401 ttgaacttta tgactcaact aataagaaaa aaggttataa catcgcaaaa ggcggaaatg
    35461 gcggaataat atataaagtt caccccaaag gaatgctagg gaaaaaacaa tctaaagaat
    35521 tttcttcaaa tcacagtaaa tgggcaaaga accataagaa taattgtatg acaaacggtg
    35581 atgttgtatg gggtgttact cacgaacacc caaaaggcat gttgggaaag caccacagca
    35641 aagaatcaat tatgaaaaag aaagcatata gtggtgaaaa tgctgtaacc tcaaaaccaa
    35701 ttgtcgcaat tgaacgggat ggaactaaaa gagaatttca cagcgctaag ctttgcatgg
    35761 cttactattc gattagtaca tgtgtttttt accgactttt aaaagatggc gccccttatg
    35821 tgataaatcc taaagcgaac tacagaaata aagaaaaaat attagctatc gaaggaatta
    35881 tgttcaaaca taaagaagat accgaggtaa gttagtagat tgcgaaaggt tactaactac
    35941 cgtaacgcgt aggagttgaa taaatataat gctcccaaga gtgtccgaca gcagataatt
    36001 tgctgaaaat gtacgctaaa ctgggccaga actgactgac cgatgaaaat gagggtgacc
    36061 tccagagtgc gagataaaaa gctcgcagat aataacaatc gaaaacatca ctaatttcag
    36121 gcctttccac atacatggtc gcttatgatg atgaacaagg cgccaacgtt tacgtattgg
    36181 caaatgctcg tgatcaagca agcttgttgt ttgataaggc cgcagaaatg gtcaaacaat
    36241 cgccggcgct ctttaagaaa tttggtaagc ctaaacgatc aagtattaat tatgctcccg
    36301 ccttttctaa aatggaacca cgcgcctcag atagccggaa attggatggg ctaaacactc
    36361 actttggtat ttttgacgag atccacgagt ttacgaatta caatctgatc aacgttatca
    36421 agaaatcaag aggaaccaga aaacagcctc tgatagttta tatcacaact gctggatatg
    36481 tattagatgg tccgttgatg tcttattttg agcaaggtgt ggactgtttg gaacatttgg
    36541 aagatgacat cgatgaacgg actttctatt atctggcaaa acttgacagt gcggaagagg
    36601 ctgatgaccc aagattatgg atcaaagcca atccgaatat ttgtctaatg aattttgttg
    36661 gcatgctaga tgactatgtt aaggataaaa aagatccaaa agaatatgct gactggatta
    36721 ccaagcaatt taacttgttt tccgatatcg atgagctgtc atttgtcgat atgcctacca
    36781 ttaaacgaaa caataaaacc atcgatattg aaacgctcaa aggtaagaag tgtgtcggtg
    36841 gttttgactt gtccgaaacg gaagacttta ccgcagccgt tttagaattt ccgcttgaaa
    36901 caggcgaggt attcattttg caacacacat ggatcccaca agctagattt gatcgagata
    36961 acaatcaaga gcgtatcaaa gcgtgggaga aggtgggaga tctaacgatt attcctggtg
    37021 attacgtcaa ttatgaatac gtcttaaatt ggtttgtaga aaattcgaaa atctatgaca
    37081 ttgtaaaaat caattatgac aaggccaagg cgctacgatt aaataaagaa ctagaaaatg
    37141 caggatttga aaccaacgag attcggcaag ggtttctatc attaggtggg ccaatgcaaa
    37201 acttcaagga aatgctattg gacggtaagg tgattttcaa caattccaag ctttaccgat
    37261 ggtatctatc caacgtcaag ctggtgatgg atcgcaactc aaactggatg ccgtctaagc
    37321 agtccaagag tagaaaaata gatggttttg cagcaagttt gaacagccac gccgaagtgt
    37381 tgaatatgtt ggttaatcct gtcggaaccg ggaaagtaac ctattactcg atttccgatt
    37441 taatgaatat gtaagaaagg tgtggaggaa tgagtatttt agatcgtttg cgttcttttg
    37501 gccgagcgaa gccgaaagcg agcaaacaag agtatttttt gaatgacccg ggattgatac
    37561 cgtatttagt cggaaaagat gaaatatcag aagggatttt ttccgtaatt agccgtgtat
    37621 cgaacgtttt tgcgtctctc cctctcaaaa tgatagatgt ggagtttggc caaccggacg
    37681 actgtcctgc atacaacttg ttgagcgaag gccctcgata ttttacaaag tttgattttt
    37741 tccgggacgt ggaagttttg agaaactacc aagggaatgc gtatgtgcag attttccgaa
    37801 atatcaatgg agaagtagca gatatggcgt tagtaaaacc tggtgcttgc catccagtga
    37861 ttgatatgga tagcggggag ctttactacc aagtaactgc gactgacaaa ggcagttaca
    37921 agcaagttat ctatgtacat tacatggaaa tgcttcactt taaacaaccg aggtttggcg
    37981 gcttggaagg tacagacccc acaaaagtat taacgaatac cctcggatat gatcgagaag
    38041 tccgaaaaat ctctttaagt cagcttaaag gaagtaatga agggctaaaa gttaagtttg
    38101 ctagcaatat ggatgaagaa gctaaaaaag ctacagttaa aaacattgct gatttttatc
    38161 gacaaaacgg tggactactt gtggaagaaa acggtgtaga aatcgaacgt ttacaacgag
    38221 agctggtaga cagcaagctt ttagatactg ataaagtatc tcgctccaga atcgctatgg
    38281 tctacaatgt gccggagcat ttcatcggga ataaccagtc gagttactcc tctcaggaac
    38341 agctcaatat ggaatttttg acgtacaatc tagtaccgac cgttaatcaa tatgaagcgg
    38401 aactaaataa gaaaacacta tcgagaaccg aaaaagcaaa aggttatcga tataagttta
    38461 atgtcgcaag tttgctaaga gctgacactc aggcaagggg acaattctat caaatcatgc
    38521 gacgaggcgg agcatactca gcgaatgatg tccgaagatt tgaggacttg caaccgataa
    38581 ataaaaccgg tatggatgat taccatattt ccggagacct atatccaatc gatatggatc
    38641 caacattaag aaaaacaacc tcgtctaaaa gcgtagccga aaacggttag gtttttttag
    38701 tttgcaccga agggaggtgg aaaaatgaaa aaagtgacgt taagcggcga tgtcgtggat
    38761 aacgataccg cgtggcttta tgactggttt gggatcaatt gtatctcacc agggaaaatt
    38821 tctgccgctc ttacagaagc agcgggggat gaagtagaac ttgatatctc atcgaacggt
    38881 ggggatgtcc tagcggcaag cgaaatatat accgctatcc gtgcctatcc gggaaaggta
    38941 tccggaaatg ttgtgagtat tgcagcaagc gctgcaagtg taatcgcttg cgcttgcgaa
    39001 ccgcttagaa tctcacctac ggcacacatc atgattcata acgcatgggt gaccactagt
    39061 ggcaacgctg aggaattaaa agccaatgca gaaatgttaa gcagtgtgga tgagtctatt
    39121 gttaatgctt acgagatcaa aacaggactc gatcggaaaa aacttgctga tttaatggcg
    39181 aaagatactt ggttaaatgc tcaaacagca gtagcagaag ggtttgcaga tgaaattatg
    39241 tttgcagaag caccagtaac ggtactcaat gcctctcaac cggttattcc aaaaaacgca
    39301 gtaactaagt tgaaaaattt aatactcaaa gcggaagcac cgcaagaaga aacactctta
    39361 cagaaaaaac taaaagcctt aaatggaggg aaaaacgaat gaatttagaa caattaaaaa
    39421 atgcgtgggt cgaggcggga agtaaagtct ctgacttaaa tgcacaactc aatgcagcat
    39481 tggttgacga tgaaaaaaca gaagaagatg tagtaagttt gcaagcacaa gtaaaagcag
    39541 cacgggctaa acgggacgga ttgaaagagc aagtggcaaa tatggaagcc gaacaagtct
    39601 taaacgtcaa aaaagaacca ttagataaaa aagatgaaaa cttgaaaaac aagtttatca
    39661 aagactttaa agcgatggtc aatggtgatc ctgctattat ggctactttg acatctaata
    39721 cggatgaatc tggtaatgct atcggattga ctattcctgt agatgtgcaa acgactattc
    39781 atactttggt tcgtcggttt gactctttgc aagaatacgt aaacgttgaa aaagtgacca
    39841 ctaccagcgg ttctcgcgtt tatgaaaaat ggtctgatat tacaccgttg actgctttgg
    39901 atactgaaga cggtgaaatc ccagcaaatg atgatcctgc actttacttg atcaaatact
    39961 tgatcaaacg ctatgcaggt atttccacag taactaacag cttgctaaaa gatactgccg
    40021 aaaacatttt ggcatggttg tctaaatgga tcgcgaaaaa agtagttgtt actcgcaata
    40081 caaaaatctt ggcagctatt gatggaatca aagcggcaca aaagaaagat gttacagatg
    40141 ttgatggaat taaagatatc gtaaacgtcc aacttgatcc agctatcgaa gctacatcta
    40201 tgtttattac aaaccaagat ggcttcaatg ttttagataa agtgaaacgt gctgatggat
    40261 cttacttgtt acaaaaagac gtagcttctg caactggata taccttctta ggtaaaccaa
    40321 tcaagaaaat tgcttctcgt ttcttgccaa acaaaggtac gcaagctact cctaaatatc
    40381 cactgtacat tggtgatctg aaagaagccg ttacattgta tgatcgcgaa aacatgagct
    40441 tgctgacaac gaatattggt ggtggagctt ttgaaacaga caccactaaa gtacgcgtca
    40501 tcgatcgctt cgatgtgcaa ctagttgatg atgaagcggt tgttttggct acttttacaa
    40561 ctattgcgaa cgagacaccg gcgaaagttt aaggagctga ttccttatga ttcttgatcc
    40621 taaaatggat ttaggcgaaa tcaaaaacgc actaaagatt gataccgatg atgacgatgt
    40681 ggaagtaagc cgtgcggcac aagctgcaat tgcatacatt agaggggcta tcgggaatga
    40741 taagccctct ttttataagc aagaaaatga aacggttgat ctgattaatt tagctatcct
    40801 gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga acttgcgaga
    40861 gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagttatt tgctttttgt
    40921 ggaggaggag tagcgtatgc cccttatcca aacaggaaat ttaaatcaac gcatcaagtt
    40981 tgtccgagat acgacggtta aggatgagga cgggcaagtt gtcccgactt ctacaaccat
    41041 tcttacttgc tgggcaagtg tgcagacaca acgcctgaac gatattaaga cgtcgattgg
    41101 tacggctttg gaaggaacac tgacgttcat tatccgctac caacaaaaat cagagctgac
    41161 caatgatatg aaagtgcgtt ggaatggaaa aacgtttgaa atcattacga ttacgaaagg
    41221 cgagtttgcg aaggacttca cgacaatcat tgcaaaagag gtttcaaaat gagtgtagaa
    41281 gtcgatgcaa ccgaagtgta caaagcgctt agggaagtaa aagcaaacgt tcaacgagtg
    41341 gaaagcccag cacttagaaa agctggggag tacgctcaag aagagttacg acaaaacaca
    41401 ccttactggg atggaacgaa gtcaaacggt aaacgtggtt cgtatatgca agaacatgct
    41461 aagaaccatg tggttacaag ctcgataaaa aacggattgg tagaagtcgg ctatgacaaa
    41521 gatgtttctt ggcggatgca ctttatcgag ttcggaacaa tcaaacaacg tccaaaaggt
    41581 ttcgtacaaa aaacacaaaa gcaaatcgaa aaacaagtaa cacaaatcat tgctgacgaa
    41641 gtaaaaagga ggctaggact ttgaaaacgg cagtatcaca agtctattca attctgaata
    41701 gcaatgaaaa aacaaagaac attgattttt acaccaatag tgttccggaa tcagctcaaa
    41761 cagtacctag ccttccagtt ggcagaatta cagagatatc cggcaactat gaagattttg
    41821 caagcaacaa tcctttgacc attcaattta acgtacaggt agatgtatgg gtgtcaacca
    41881 tgaaagaggt tgatgccttt tattttgccc ttgatgaggt tatgaggggg aatggttggc
    41941 aatgcgcata cacggaacaa acagatgacg aggacttgga aggtgcaaag cggattatca
    42001 aacgatatgt agcaaatatt tcactaaatt aaaaggagag aaaatagatg gcaacagtag
    42061 gattcgagag cgtcattttt ggcgtaaaaa caggtgcagg cggcactcta aaagaattag
    42121 tagcagataa gtcgaaaggc ggagcgatcg aagctaaaat tactggatta ggcgcaactt
    42181 ctaacacaac atacgcttca aacgtaccgt tcttcattgc aagtaaaggg gtttcgtcgc
    42241 caaaagttac gcttgacgtg gcagacttaa tggataacgg catttacagc gaaatcattg
    42301 gtgctaaaac cgtggatggt gcaaatgtaa ttggttcaga aactgaagcg ccttacgtgt
    42361 cggtagtcat ggttacagcg aacaaagaag gaaaacgctt gttcatggga ttgacaaaag
    42421 gaaaattcag tcatccagat atcgacatga aaacagctga agacaaaggg gtagaattgc
    42481 aaaccgattc catcgaaggg gaattcattt ctgatgaacg tggctatgta tacttaacag
    42541 ccgtagaatc agaaggaatg accttacaaa aattcaagga cttggtaaat aacaaagagg
    42601 gggagtagtt aaccctgcat ctacaccaac gacagataca gggacaccaa aagaaccaga
    42661 accaaaaatt gatacacaag gttagccatt tttggctagc cttatttttt gtaaaaacaa
    42721 ggaggaaaac aaatgattga attgcaattg aaacttgacg gaaagaaaaa aacattcaaa
    42781 caacaagata tttccgcacg tgcaatgcgt gagtgtatca aattttacga gaaagcggaa
    42841 aaagcagacc taactgattt agaagcgatt gattcaatga ttgcaattac agcagatatt
    42901 ttccaagatc cagcagttac atttgatgct attttagacg gtttgactgc gagcgagtta
    42961 gtaccggcat tagaaagtgt ttttgaacaa ataaatgaac tgggaaacaa tgaaaaaaag
    43021 cagacggcga gcaaaaagaa ataagttttt ctgaagctag gaaagcaatg gatcaaatct
    43081 acaaagattt aatcgaagca ggttggacga tgagagatgt ggacgaagcc gactatcatt
    43141 atttgttaca cctttttgga gaagtggaga gtggcgaaga atatgtagat ggtgctgatt
    43201 tcatcaaaca atttttatcg gctgaagact tagtaaaact tgaggaagga ggtaaataat
    43261 ggcaggaaaa ggacaaccgg caggaaatat caagctaggg attagtttag atagcactag
    43321 ttttggtaac acgctggacg aaatcaatgc gaaagtcaaa caagctgagt cgaatatgcg
    43381 tgccaatcta aaggcttatg attcagcagg acgttcatac gaagcactta gtcaaaagac
    43441 gaaagacttg tctacggtta tggaagggca aaacgccaaa gtaagagaat taacaaagcg
    43501 ccgtgatgaa gcgattagca agtatggcga ggaatcgaaa caagttgcta accttaacac
    43561 acagataaac aatgctaccg caaaatataa tgcttacagt cgccagttga acgacacaaa
    43621 aaaagaattg gtgtattcca aaacagccgt caatgattta tctaatgaaa tcaaagaaaa
    43681 tgaacgacaa atgaacgccg aagtaaaagc gttgaaagcc gctggtgatg aatctggtgc
    43741 ttttgaagca aaacaaaaag ggctagccaa acaaacggaa ttatccgaga aagctatcga
    43801 agaacagcgc aaagttgtga aactgatggc tgatgagttt ggcgattcag caaatgaaac
    43861 cgaagatgca aaaagggcat tagaaaagtt agaacgacaa agccaaatat ctagcaggca
    43921 attagaagca ctcaaaagct ccagcgatca atcaggaaaa gaaatagaag attttggcga
    43981 caagtccaca aggtcagcta ggaaactgga cggactaaaa gacaaattaa gctcgctaaa
    44041 aagcgcattt tcgtttggtg cagttgctgg attagcgcat aacgctatta gcagtgtagt
    44101 aagtggcgtg caaggtttgg ttggcgaagc agtaaacgca tcggattcat tgatgaagtt
    44161 ttccaaaacc atggagtttg ctaactttgg gaagtcacag atagaaagct cgaaaaaaga
    44221 aatgaaagac tacgccgata agacggttta tggtttagaa gaaattctga acacaaccgc
    44281 acaattggca tctaatggga ttcctaacta tacagaacta accaaggcgg caggtaactt
    44341 gaatgccgtt gcaggcggtt ctagtgatac attcaaatcc gttgccatga tgctaacgca
    44401 gacggcagga gctgggaaac taacaactga aaactggaat caattagcag atgcgatacc
    44461 gggtgcttca ggattgttac aagacgctat gttgaaaaac ggagcttata caggaaactt
    44521 ccgtgatgca atggcgcaag gtcaaatcac ttccgacgag ttcaaccaag caattgtaca
    44581 gttaggtatg aatgacggag cagttaaggc agccacttct acagacacat tgagcggttc
    44641 ttgggagcag atgaaatcca ctgtaataaa tgggctacaa agtattatag aaaaaatagg
    44701 cgttgaaaat atcactggtt tcatcaacag agtaacaaaa gggattgaaa attctattcc
    44761 taaaattact caatttatag gttggttgag agatattgga acgtggatcg ttgaaaatag
    44821 agagccacta acatggattg tcggaatcat aggcggaatt acattagcag taaaagcatt
    44881 gaacgtagca agtatgttgc tggcaattac tggcggaaca ttggcagccc cttttgtggc
    44941 gattggtgta gcattaggcg cactagcagg tgctttggtg atagcttata caaaatctga
    45001 aacatttaga aatatagtca atgcggcttt tacagctgtg aaaaacgtag ttatgagcgt
    45061 tatcaataat ttggtggaat actacaaaat gttgtggagc gtgttgcagt ggctttggga
    45121 aaaaataaaa gaatgggctt catggattgg caataaattc attgaaatga agaacagcgt
    45181 tgtgaatacg gttcagaacc tatggaatgg cgtgaaaaac ttcttcagca atggcgttgg
    45241 agatacgtgg aataaggtag tcggttgggt aaaaaacatt ttcaacaaag caactgaatt
    45301 gaagaacaac gtttctgatg taatcggtaa cctgtggaat ggtatcaaag acacattccg
    45361 tagaggtatc gatacggtat tcaattggtt ttcagaacta ccaacgaaga tgaagaatgc
    45421 cattattggc ggtaaaaacg ccattgttga tgcgttcaaa agtattttca acgcagcact
    45481 taaagcgata ggtaaaccag ttaacgcaat catccatgga gcttcatggg tactagaaaa
    45541 attgggcgca gaacctctac aagaatggga tgtaccacaa tacgctacag gtacaccagc
    45601 aggtgggcac ccaatcaatg gtccaatgat ggttaatgat ggacgtggag cagaaacagt
    45661 tatcacacca gatggtagag ccttcattcc taaaggacgc aatgttgttc taaacgcacc
    45721 aaaaggaacg catgtcttga cagcagaaga gaccgcgcaa cttcaaggtt ctaaagctcc
    45781 taagtatcgt tacaaaaaag gtactaactt ctttggtaac atgtgggata gtgtgaaaaa
    45841 ggttgctggt aatgtaggtc acacaattaa aaacgtagta ggtgacgtgt gggactttat
    45901 ttcagacccg ggagcgttag ctagaaaagt acttgggggg ttagatgtat taggtggatt
    45961 gacaaagtat ccattagaag taggtaaagg catcctatct aaagcaacaa gtgcactgac
    46021 tgaaaaaatc actgggttgt tctcatctgg taacttagat acctccgtag gaacaaatgg
    46081 cgtctataaa tatttggctg atgttgctaa gtctgtgatg aagaaattcc caggatttgt
    46141 ggcaactagt gggtatagac caggtgaccc ttattcacat ggtaaacgta atgccattga
    46201 tattgcatta ccaggcgtca caggaggctc acctagatac acagaagcag caaactacgc
    46261 ttttgacaaa ttcgcttcca aaattggtta cgtaatcact aatgggaaag ttcgtgaccg
    46321 ttcaggacaa tcaggtcaac cagcaactgg tgcatgggag ccatggcccg atggagatca
    46381 ctatgatcat gtgcatttaa acggtgtgaa agacccacaa aacactcaaa tttcaggaga
    46441 tagcgtggga ggcagtgggg tagaaagatg gcgcaatgta gcaattagag cgttgaaaat
    46501 gaccggtcaa tacagtactg caaacttaaa tgcattacta aatcaaatgc gtacagagtc
    46561 aaatggtaat cctaatgcag ttaacaattg ggatattaac gccaaaaatg gaacaccatc
    46621 aaaagggttg ctccaagtga ttgacccaac attcagacag tatgcaatgc caggattcaa
    46681 cagcaatatt tttgacccac tatctaacat cttagcttca atcagatacg cactatcaag
    46741 atatggctca ctaacaaatg cctatcgtgg agttggttac gcaaacggtg gaattgtaaa
    46801 ccaacatcaa attgcggaaa tcgcagaagg aaacaagcca gaaattatta ttccgttaga
    46861 taaggctaaa cgatcaagag cgatgcaatt attgtctatc gcaatggaca aactaggagt
    46921 ttctcctaaa acttatggaa atgcgacaac tgtttccagt gattacgaaa tcctagaagc
    46981 cattgaaaaa caggcggcaa caacgaatca gctgttatcg ttattgctgg catttttcaa
    47041 aggaaatagc cgaactgata gagatttagc tttagatatt cagaagatct tggttaggag
    47101 gatgtagatg cgaactgtat tactgagaaa tagaataaac gaagaaattg atttgtccac
    47161 agaagactat tttgctactg ggttgagtaa tatggggttt gaagtaaaaa aagaacatgt
    47221 gggacagtgg gggaatttca gagaaagcag tgagagcgtt gaaatatctg aatttcagtc
    47281 atctgtaatc atttctgtgc atgggtttcg agaaaaagaa ctgtacaatt cacttgtgca
    47341 gttcctatcg gaaggtccgt ttgaactgga atttgctttt gacggcgaaa caatggtaag
    47401 aagatgcagt ctaaaatctt taagtaagac tgaaatcgat ccgaaaacat cgttactaac
    47461 agacacttta gaactatatt tcacatcaaa ctggtattcc gtaaaacgcg aaaaattaat
    47521 tcagcgaccg aatgtagtga aaacacgtgg taaggttttt ccgtataaaa gatcttatat
    47581 ctacactcaa aacttgtggg agaaaaaagg tgtattcaaa ttcaataata attctgtata
    47641 cctaacgaat agcaaggaac ggatgtcccc gctaaaaatt cgtgtgattg ggaagtgttc
    47701 aaatccgtat tgggaagtaa tccaaaactc acaaatcatc gcaacggatg gatacttcat
    47761 agatatgact gaaacacaga ctctagaagt atcaagcctt tttgaagata caacggcgat
    47821 tttaaaagat attgcagggg tagaatcttc tgtctaccaa caacaggatt atacaaaaac
    47881 taattttgtt caggccccaa ctggagaatt cagcattgtg tttcatgttg ggggggcaga
    47941 cgtagagatt gagttatacg aggagcgtga tctgttttga ttttagcagt cacgcttttt
    48001 catcgtgatt taatgcttta taacgaacat ttattctcta acagttttga atttggtgtg
    48061 gacgagatca acgaagaggc tagtagcttt acgatggata agtacgttcc tgtaaaaaca
    48121 ggcgattttc tactagcaaa atatattcct agtggaaaat ttgcatattt tggtgtaatt
    48181 acgtcgcagg aagacgaaaa aatcagttgt aaaagcttac ttagtttagc tgatagtgag
    48241 ataccgactg cacgtgtgtc aggagataac tacgaagagc atatccggcg gttgattgaa
    48301 tactatttgc tgaatgatcc aacgaaacaa ctaaaagata ttttagacgt caaagcagaa
    48361 agtgcgacct ctcattcgta tcaagctacc gatacgaata aacacaagtt aagtgcgtat
    48421 attctcaatg gtttcaaaaa atacaacgta aaatggtatt tcaagggaat tcaaaacaga
    48481 aaaatttata caggcatacg tgctgtaaat gaatcaattt acattaaaga caattcttct
    48541 gaatttagcg attgggatgt gtttgttcag gcgccgggcg ctggaaacga gaacaagcta
    48601 ttaatcgttg ataaagcaat gaaagatata gagaaaccga taatactgtc aacatggtat
    48661 ttggacgaag aaaacaattt gacacaagat ggatcgaaag aaaatatcac gaaaccaact
    48721 gtcaatttgg ttaatatcta cgatcaaacc gcagaagata aagcatccta cgaagatgtg
    48781 gcaaaatcag agttgaaagg caatacgtat tcgcatgaaa tcaaagtgaa cgttgtaaga
    48841 aatgcaaaaa atttgaatgt cgaaacgatt gaaacaggga tgtttgccac gatttcttat
    48901 aaaggaaaga tatacaagtc ggttttgaca gcttggcgaa tatcaagtga taaggagttt
    48961 gtggaattga ctttcggaaa cataagaagt cgttttatgg attattttga agataatggg
    49021 ggataaaaaa tggttagcaa tgtggatgga tatcaatttg aaaacgtgaa agtaagcgca
    49081 gaaaatgatg ctagacttta tcacgtttta tataatcgga aaaatcaggt tattgatggt
    49141 tacgatcagt ctatgaattt atcttcaagc ggattaacag taaaagttgc tgcaggagca
    49201 gcgattattc aaggtcgtat ggtcgttgtt cgacaagaag aaagtataac agttccagca
    49261 aactcaagtg gttatatagc attaacagtg gatttgacac aagaagttat acctggatct
    49321 attcttccag aatcggaaga atatgaatgg actaataatc aagtcaagct agaatttata
    49381 acaaaagtta taaaaggtaa tttaaacaat ggtgataagg tctataattt accactatgc
    49441 tcagttaatt ctactggatc aactgtttca atctcaaaga tatcggatag ttacgagctg
    49501 actctctcta aaggagaaat tttgtggagg gggactgcgt taatgcatga tactcaaact
    49561 gtccaacctt caaaaaaaat ttggcagaca gtttcaggtt ttttgttagt atggctccca
    49621 tacgaaaacg ggcaagcaat tgaggataga tatgtaacta cgccttttta taaggagcgt
    49681 gtaactttta ctaatgtttt aggagaaatt gtttcaggat ttgatgacta tcacaaaaag
    49741 tggtttagta aacgaataaa ctataattcg aatactaata tatttacagg tgcagcaagc
    49801 aatgcaagtg gagataacgc aaatatggtg cttaggtata tagtttcttt ttagttagga
    49861 ggtatggaag agtggcaaat ttagaaatta aattatctgc aaataagaga cagccttatc
    49921 tacgtcaccg tgttgttggt agagttggtg atggggggct cacaacaatc aatgtacaac
    49981 ttcttgagga agatgaaatt acaccttttg taattaatct aaacggtact ttgaaatttg
    50041 tgggcgaagt ttcaaacggt aactataccg agggagaacc agaaataatc gattcgacta
    50101 atgggttaat tagttacacg ttcactaagt caaatttcag cacgagtcat caattcaaac
    50161 aagcatattt tgaatatgta gatcctaacg gcaaaaaagt aacttttcag aacttcatca
    50221 tagacgtgtt agaacgagtg gatattaatt cggagcaagc gaaatactat atttcttcat
    50281 tggaaaaatt acagagtgaa atgcaaacca ctttcaatca gttcattagt gataaacagg
    50341 tccaatacga tcaaatctac tcgaaatata acgaattagt aagattgata aatgaatcag
    50401 ataagcaagt aaatgatcgt attgatcaaa ccaatcagca aatcggcgat ctcggcaagc
    50461 tgaaaaagat gtacagtaac agcatcgact ttgggggcta tgattattcg gggaatccga
    50521 atttgttaag taagctatca tacgacttaa ttgaaaatca aaatacttca gctggaacac
    50581 tttctaaggg tgaaaactcg tttaaatata ataagatatc agctgaagtg gaaggtggag
    50641 tagagttata ttataaacga agaggtatag ctaactggtt accctctaat aaaacgcttg
    50701 taatgactgt taagcttaga gctggagcgg actatagtcc agttgacgga aaacttatac
    50761 tgattagata taggtatgtt gacagtggaa ctggcaagat tgttttagac ttacctatta
    50821 acagtaattc gataactcag gaatggaaag agtttagtat tactggaact actccaacat
    50881 ttagcccaca agcataccat ccttggatac aatttagggc tcaagatggg gtacttgggg
    50941 aaatagaaat gagctatgac atcaaaatcg aagaaggctc tacagctaca ccatatcagc
    51001 ctaacttact tgatgactct tactggctag gtaaaacacc gttgggtgag aatttgataa
    51061 cgaatgccaa gttcccaatt ataacacgaa gcaatcctat ttctggtttt gatatttcag
    51121 aagaattaat aataggtgaa acttatacgg tatctttaaa agggactaag ccagctacta
    51181 aagaattcca cttatactac ggagaagcaa attaccagca gttgcagtat caggcaactt
    51241 tacttcctgt ggaaggatta acagatgtgt ggagtgccac ttttacagcc agaaatacta
    51301 gtgaaacgac aaatttactt agagtagcgt tatggcaaaa acctaattct gcaacatatg
    51361 atactgttca aattgactgg ctcaagattg aaaaaggcga cacacgcaca ccaaatatca
    51421 gtcagtttaa atactttggt gaaggcttga aagacagtaa caatccgaac gactacagct
    51481 gggatgtcac accagagtat actgaaaaaa gcttgaataa tacggttagt ctgactgaac
    51541 cgcaatccat cgaaggattg aaaaatttcg aagatggtat tcaatcaaaa ggaaaatctg
    51601 tattgacatc agacgacaac aaatatgaag tcgtaacctt aacagttaca aacgggaata
    51661 ccggatcagc aaagctttat cgtgaaggaa aaaccgtcac tatttatttt tttgcgttga
    51721 atgggaaaag cagtggtgga aatgattcaa cgatactaac aattccagaa ggctatcggc
    51781 caccaattag ttttgagcaa ctggttggct cgatagaccg ttctactttg aacagtgctc
    51841 agttatctat tggtgcagat ggagccatta aatggcgaag aaactcaagt tatggatcgg
    51901 attatacctt tgcaattact tacacgattt agaaaggcgt gaatcgatat gaaggcagca
    51961 tatagaccaa ttgaacctta cggattcgag caaatcattg tgaatgatga agaacattta
    52021 ccggaagaat gcacagaagt cgaaccaccg attccaaatt ggaaaccgaa attcaattac
    52081 tgggagggaa ataaatgaaa aacatttgga aatatggccg tactggcgga gagtatgcag
    52141 gaaaagtatt ggacgacatg cttgtatccg ttccttacac agatcagcca ccgctcgaag
    52201 gggttcgttc agatggcgaa ccgctaacga ttgctgatca gatgtttgat cctaaattga
    52261 accaatggat tattttagcg aacgcgttag atcacaacga tttaaacaat ctcaaagcga
    52321 tgtacgaggc tcttgaacat gaaaacggca acctaaaaca gctaaatgcc aaactcatgc
    52381 taagcgatgt agcgattaaa caggaaaata ctgcattgaa agaaaaagct gacagtttag
    52441 cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa tagcaaagac atttcagaaa
    52501 ttaaagagca actaaatcca gcttcaaagg gaggtgagta gtatgtttag ttttagcgat
    52561 gtgaaaatga tgtatgattg gggctgtttt actgacgatc aagttcgtct attcgttcca
    52621 ctatgtatca cagatgaaga agcagataga attattagca aagaagagag cgcatcttaa
    52681 gtgatgcgtt tttttgttgg aaagttggtg gaacatgaaa gaagaagcgc tccaagacgt
    52741 tgtggagaga ttagtaagaa ttgaaacaaa attagacaac tacgaatcac ttagagaaaa
    52801 agctgatagt gcaaaagatt tggcagacaa agcctactca gtagcgctaa acaacgcaga
    52861 agatatcaaa gaaatgaaag ccaataataa atggtcgtgg ggttacatga tcggtttagg
    52921 cattacgatc attggctatt tcttgactaa attgtaaagg agttaagaag aaatgatttt
    52981 acccgataag tattatcaag tcattaaatg gacggtttta acagtattac cagctgcatc
    53041 tgttttagta gccacgttag gaaaagcgta tggatggaat ggaacagata tgacagtact
    53101 cactatcaat gcagtagcaa catttttagg cgttatcact ggtgtgtctg catataactt
    53161 aaaaaaatag gaggaaaaaa atgaagaaga aaatcatttt atcattgagc ctaataatgg
    53221 ctcttttttt attgccaatt aacgggttcg cctatacgat caacaatgaa tttaatttag
    53281 gtgtaaatga aggtagctca caagtagcaa ataaccaata catcctattg catgaaacag
    53341 caaacgaaac tgcgacagga cgaaatgaag cgcagtacat gaaacgttct tggtacaatg
    53401 cctatacagc gtatattgtt ggcgatggtg ggatcgttta ccaagttgga caacctggtt
    53461 atgtacaata tggcgctggt tcatatgcta atgcaaatag tcctgtgcaa atcgaactgc
    53521 aacatacgca cgataaagta acgtttgaaa agaactacaa ggcatatgtt gaattagcga
    53581 gagattccgc taataaatac ggtattccac ttacattaga cacaccgtat aatcaaccag
    53641 gaatcaaatc acatttatgg gtaactcaaa acatttgggg cgatcacact gatccgtatg
    53701 gatacttatc agaaatgggt gtaagtaagg aaaaactggc ttacgattta gctcatggat
    53761 ttacagacga aaacccaacg acttctgaga acaagcctgt cattgatcca acccgagcag
    53821 gtgcagcaaa tccaacttta tcagatggaa cgaaccattc tcacattgat cagtttggag
    53881 aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat cgctaactat aaatacgagt
    53941 atattttcat tatggactac aatactggga aagaattagc tcgagtaaga gctgatggaa
    54001 tttatagacc ggatgtaaat caagcttata atacttctgg aaacgttggc tatcatgtat
    54061 cttttaacat gcgcaatttc cctaataaga aagtatacgt catgatgcgg gcaacgaatg
    54121 atccagaagg aaacactaaa ggcggtgcgc aagatttcca tgacaaacgt tggtatttaa
    54181 atattcctaa acgataaaaa tagctcctcg ttgaggagca gtacatagaa ttgaaaatta
    54241 acgttaaatc aaaaaaatat ttacaagatg tttatttaag tagataataa aagaaaaaag
    54301 caggtgaaat tgtgacagca gaaattggta ttatgaataa aagtggaatc gtgttagctt
    54361 cagattcagc atctacgatt ggagatagca aagtatataa tactgcaaaa aaattattta
    54421 ctttggattc catgcattct gtaggtatca tgatatatgg taatgctgaa tttaatggta
    54481 ttccttggga aataatcatt actcaatata agaaaagcat tggtagttct gtttttaata
    54541 ctttggaaga atacgcggat aattttatag aatttgtcaa aacagcatct tttattagaa
    54601 gtgaacagac cgagcaagaa caaatgattg gggtttttca aaaaataatt tcaggtttat
    54661 ttgagagtat agaagtagat attaactttt taataagcca aggtacacaa atagacaaag
    54721 atgtcctagt gaagttatta caaacaaaaa tgaataccaa tttgtctcaa cagtcccaga
    54781 cattcatttt agatattgaa aaggcgttat ttcttagtaa ctatggagaa atattaaaag
    54841 atattcttaa tagtatttct acaatggaag gtgtttcaga agctatatca gaggaaattc
    54901 aaagttacgt ttatgaaatt ataattagag acgatgttta ttcttcccct acaggaatag
    54961 ttatagcagg atatggaaga atggatattt tccctaaact atattcttat aatatgtttg
    55021 gatttgttat gaatattctg aaatactcag aatatgaaag tgctcaaata ggaaatgaca
    55081 atggttcatt aagatctact atattaccat ttgctcagtc tgatgtggta aatactgtag
    55141 ttcaaggggt agatccacaa attactaatt atctgtcgag tcaagtcgat agttttgacg
    55201 ataacggaaa aaatacctat attaatatta taaagaatat ttcagagttt caacaaaatc
    55261 aattcatatt tccgttacta aacatgattg ctttactccc agttgaagaa acagcaatta
    55321 tcgcagagac tttattaaat ttaaccagtt ttaaacgaaa atatactact tctgtagaaa
    55381 cagtaggtgg cccaattgat gttttagcta ttacacctaa tgatggccct atttggatta
    55441 aaagaaaaca ttattttgat attgataata atattggata cagattgcga aaggagagag
    55501 cgaatgatta caataattaa acctcaggaa aaatcccctg ttttgaaatt taatgagagc
    55561 aatctagccc ataataaaat gagtataact gatacagtat ttgatgtaat ggttgagcaa
    55621 caaaaaaaaa ttaataataa caatagacaa agaaaagaaa ttaaccaata ttaatgtttt
    55681 ttctgttgcg ttttttgcag tcataattta aaacctcgct caatcttggg cgaggtcttt
    55741 ttttattttt ctgaaaagtt aaagtaaata atagcataaa ataatatatt ttacaaagaa
    55801 taagtacaat ctagtttttt gctattaaat gtgtaataat aaatatgcca tcacaacatg
    55861 aagaatgaaa tccattatta tctagtctag tccattcttt ttgtttgcag tatttgtgat
    55921 ggctttc
//
LOCUS       DAYYBH010000011        54450 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000011 DAYYBH010000000
VERSION     DAYYBH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.011"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACHAUG_000777"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACHAUG_000777"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8479889.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACHAUG_000778"
     CDS             complement(1034..1954)
                     /locus_tag="ACHAUG_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8479890.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACHAUG_000779"
     CDS             2067..2819
                     /locus_tag="ACHAUG_000779"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8479891.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3683..4216
                     /locus_tag="ACHAUG_000780"
     CDS             3683..4216
                     /locus_tag="ACHAUG_000780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8479892.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4706..5230)
                     /locus_tag="ACHAUG_000781"
     CDS             complement(4706..5230)
                     /locus_tag="ACHAUG_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8479893.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5223..5696)
                     /gene="tsaE"
                     /locus_tag="ACHAUG_000782"
     CDS             complement(5223..5696)
                     /gene="tsaE"
                     /locus_tag="ACHAUG_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8479894.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5819..6799)
                     /gene="pta"
                     /locus_tag="ACHAUG_000783"
     CDS             complement(5819..6799)
                     /gene="pta"
                     /locus_tag="ACHAUG_000783"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8479895.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6952..7632)
                     /locus_tag="ACHAUG_000784"
     CDS             complement(6952..7632)
                     /locus_tag="ACHAUG_000784"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8479896.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7758..8627
                     /locus_tag="ACHAUG_000785"
     CDS             7758..8627
                     /locus_tag="ACHAUG_000785"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8479897.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8653..9168
                     /locus_tag="ACHAUG_000786"
     CDS             8653..9168
                     /locus_tag="ACHAUG_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8479898.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9215..9778)
                     /locus_tag="ACHAUG_000787"
     CDS             complement(9215..9778)
                     /locus_tag="ACHAUG_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8479899.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9833..10012)
                     /locus_tag="ACHAUG_000788"
     CDS             complement(9833..10012)
                     /locus_tag="ACHAUG_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479900.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10089..10598)
                     /locus_tag="ACHAUG_000789"
     CDS             complement(10089..10598)
                     /locus_tag="ACHAUG_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479901.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10694..11074)
                     /locus_tag="ACHAUG_000790"
     CDS             complement(10694..11074)
                     /locus_tag="ACHAUG_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8479902.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11404..12153
                     /gene="pnuC"
                     /locus_tag="ACHAUG_000791"
     CDS             11404..12153
                     /gene="pnuC"
                     /locus_tag="ACHAUG_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8479903.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12474..13691)
                     /locus_tag="ACHAUG_000792"
     CDS             complement(12474..13691)
                     /locus_tag="ACHAUG_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8479904.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(13949..15226)
                     /locus_tag="ACHAUG_000793"
     CDS             complement(13949..15226)
                     /locus_tag="ACHAUG_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479905.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15305..15949)
                     /gene="pcp"
                     /locus_tag="ACHAUG_000794"
     CDS             complement(15305..15949)
                     /gene="pcp"
                     /locus_tag="ACHAUG_000794"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8479906.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16148..17092)
                     /locus_tag="ACHAUG_000795"
     CDS             complement(16148..17092)
                     /locus_tag="ACHAUG_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8479907.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17094..17780)
                     /locus_tag="ACHAUG_000796"
     CDS             complement(17094..17780)
                     /locus_tag="ACHAUG_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8479908.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18254..18574
                     /locus_tag="ACHAUG_000797"
     CDS             18254..18574
                     /locus_tag="ACHAUG_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8479909.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18728..19633
                     /locus_tag="ACHAUG_000798"
     CDS             18728..19633
                     /locus_tag="ACHAUG_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8479910.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19630..20403
                     /locus_tag="ACHAUG_000799"
     CDS             19630..20403
                     /locus_tag="ACHAUG_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8479911.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20584..22008)
                     /locus_tag="ACHAUG_000800"
     CDS             complement(20584..22008)
                     /locus_tag="ACHAUG_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8479912.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22219..22665
                     /gene="nrdI"
                     /locus_tag="ACHAUG_000801"
     CDS             22219..22665
                     /gene="nrdI"
                     /locus_tag="ACHAUG_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8479913.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22730..24712)
                     /locus_tag="ACHAUG_000802"
     CDS             complement(22730..24712)
                     /locus_tag="ACHAUG_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8479914.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24726..25622)
                     /gene="murQ"
                     /locus_tag="ACHAUG_000803"
     CDS             complement(24726..25622)
                     /gene="murQ"
                     /locus_tag="ACHAUG_000803"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8479915.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25625..26686)
                     /locus_tag="ACHAUG_000804"
     CDS             complement(25625..26686)
                     /locus_tag="ACHAUG_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8479916.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(26897..27424)
                     /locus_tag="ACHAUG_000805"
     CDS             complement(26897..27424)
                     /locus_tag="ACHAUG_000805"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8479917.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(27491..28993)
                     /gene="eat(A)"
                     /locus_tag="ACHAUG_000806"
     CDS             complement(27491..28993)
                     /gene="eat(A)"
                     /locus_tag="ACHAUG_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8479918.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29251..30372)
                     /locus_tag="ACHAUG_000807"
     CDS             complement(29251..30372)
                     /locus_tag="ACHAUG_000807"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8479919.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30498..31439
                     /locus_tag="ACHAUG_000808"
     CDS             30498..31439
                     /locus_tag="ACHAUG_000808"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8479920.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31589..32485)
                     /locus_tag="ACHAUG_000809"
     CDS             complement(31589..32485)
                     /locus_tag="ACHAUG_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8479921.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            32754..34094
                     /locus_tag="ACHAUG_000810"
     CDS             32754..34094
                     /locus_tag="ACHAUG_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8479922.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            34312..34755
                     /locus_tag="ACHAUG_000811"
     CDS             34312..34755
                     /locus_tag="ACHAUG_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8479923.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(35259..36677)
                     /gene="nhaC"
                     /locus_tag="ACHAUG_000812"
     CDS             complement(35259..36677)
                     /gene="nhaC"
                     /locus_tag="ACHAUG_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8479924.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(37080..38303)
                     /locus_tag="ACHAUG_000813"
     CDS             complement(37080..38303)
                     /locus_tag="ACHAUG_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8479925.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(38334..39173)
                     /locus_tag="ACHAUG_000814"
     CDS             complement(38334..39173)
                     /locus_tag="ACHAUG_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8479926.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(39190..39870)
                     /locus_tag="ACHAUG_000815"
     CDS             complement(39190..39870)
                     /locus_tag="ACHAUG_000815"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8479927.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(39872..40945)
                     /locus_tag="ACHAUG_000816"
     CDS             complement(39872..40945)
                     /locus_tag="ACHAUG_000816"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8479928.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(41001..41239)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(41282..41515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(41592..42263)
                     /locus_tag="ACHAUG_000817"
     CDS             complement(41592..42263)
                     /locus_tag="ACHAUG_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8479929.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(42350..44998)
                     /gene="mgtA"
                     /locus_tag="ACHAUG_000818"
     CDS             complement(42350..44998)
                     /gene="mgtA"
                     /locus_tag="ACHAUG_000818"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8479930.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(45340..45514)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            45687..46124
                     /gene="ndk"
                     /locus_tag="ACHAUG_000819"
     CDS             45687..46124
                     /gene="ndk"
                     /locus_tag="ACHAUG_000819"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8479931.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(46146..47012)
                     /locus_tag="ACHAUG_000820"
     CDS             complement(46146..47012)
                     /locus_tag="ACHAUG_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8479932.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(47172..49265)
                     /locus_tag="ACHAUG_000821"
     CDS             complement(47172..49265)
                     /locus_tag="ACHAUG_000821"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8479933.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(50014..50637)
                     /locus_tag="ACHAUG_000822"
     CDS             complement(50014..50637)
                     /locus_tag="ACHAUG_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479934.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(50796..52193)
                     /locus_tag="ACHAUG_000823"
     CDS             complement(50796..52193)
                     /locus_tag="ACHAUG_000823"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8479935.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(52225..53196)
                     /locus_tag="ACHAUG_000824"
     CDS             complement(52225..53196)
                     /locus_tag="ACHAUG_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8479936.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            53570..53995
                     /locus_tag="ACHAUG_000825"
     CDS             53570..53995
                     /locus_tag="ACHAUG_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479937.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        15718 a        11284 c         9619 g        17829 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     3061 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3121 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     3181 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     3241 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     3301 cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca
     3361 aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca
     3421 ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg
     3481 aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca
     3541 tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat
     3601 aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat
     3661 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     3721 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga
     3781 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     3841 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     3901 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     3961 ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg
     4021 tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt
     4081 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     4141 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     4201 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     4261 tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt
     4321 gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct
     4381 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     4441 ttggacttat taccgcagat gcaagctttt ggacaccgtt taccaagagg ttgtgaaatc
     4501 agcgttttga cctgagtatt agagaaacaa acggcaaaat agttcctcat attttgccgt
     4561 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttattcaaaa aaaatggact
     4621 gcgacacgac ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa aaggatgaca
     4681 gtttctcact cttttcctat ttcttctatt caatcaaata actcatttta tatacatcta
     4741 aaaattcacc tagatctgtt cttgcccctc taggcatgac cgcttcgatt tggaagccca
     4801 tttttcggta aaggtgtacg gctctttcat tacgaacttg aacgtcaagc tccaatcgaa
     4861 aaagtacacc catttccttc gcccaactga tgatttcttc tagcataaga gtgcctagtc
     4921 ccattcccca atattcctgc agaatactga taccgacttc tcccacgtga gacaaacgaa
     4981 attgactgtc tgccttgaca gatgctgttc caataatcgt cccttcatag attgcaagca
     5041 gcaataaatt attattgctt tcgtatatat aatccaactc ttcttctaag gtttctggtg
     5101 gtaacaggag ttctgcttca tccaaaacta aaaattctgt ctccttatta acttgtccca
     5161 tcatcaaaag aatgtctttt gcatctcctg gcactgcttc ccgcaagatg atcccaactt
     5221 cttcactcat gattttcagc ccatttttct agaattcgct gtttgaaggt ttcactttgc
     5281 tccccgtatg agattagttt cacataccgc ttttctaaat ctgtatcact tttttctaaa
     5341 atcagttcca aataatcctc cggcagtgct tcttctaata gatttcccca ttcaacaacg
     5401 gaaagcccat caccttcaaa atattcatct aaccctaaat ctgctccgct agctgctacc
     5461 cgataaatat ccatatgata caacggaatc cgaccttgac tatattctct aataatcgtg
     5521 tatgtcggac ttttgatcat ttgcgttatc cctaatcctt gggcaattcc tttcgtcaac
     5581 gttgttttac ccgcacctag atcaccagtc aatatcagat tgtcacctgg cattgcggaa
     5641 tgaccaattg cctctgcgaa ttgttctgtc attgttaatc ctgatagttc gatcattttt
     5701 catcttctcc cgctcttctt tctttttgcc agtatatcgc gtaatgaagt ttttttaaag
     5761 aaaaaatgaa ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg tgtatttgtt
     5821 aatcaatcaa tgtttgtgct gctgtgataa tcgataattt atatacatct tcttcatttg
     5881 ctccacgaga caaatcagaa accggtttgt tcaagccttg tagaattggt ccgattgctt
     5941 caaagttacc gaaacgctga gcaattttat agccgatatt tcctgactgc aattctggaa
     6001 atacaaagac tgttgcttga ccagctactt ctgaatctgg cgctttcagt tgtgcaactg
     6061 aaggaacata agaagcatca aattgcaatt cgccgtcgat catgtattgt ggcgctagtt
     6121 ttttagcgat tttagttgct tcaactactt tgtctacttc tggcgatttc gctgagcctt
     6181 ttgtcgagaa gctcatcatt gctacttttg gttcgatatc gaacaattct gctgtttttg
     6241 cagaatctac ggcgatttca gccaattctt gcgcagttgg attcacgttg atcgcacaat
     6301 ccgaaaagac atatttttct tgatctcttc cacgtaccat gatcatcgct ccgcttgtcc
     6361 gactgatgcc aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg tcgccagtcg
     6421 aatggatcgc tccagagacc attccgtctg caatccccat atacgtaagc atcgtaccaa
     6481 agtaattcac atcttttaaa atcttttctg cttgttcttt ggatgccttt ccatttctac
     6541 gttcaacaaa agtatccacc atttcatccc acttatcgta attatcaggg ctgatgatcg
     6601 taaagctaga tgttttgatt cctctggcat gcgctgcatt tcttatttct tcttcgttcc
     6661 ctactaaaat gggttccaca agttcttctg ctttcaaccg ggcagctgcg cctaaaatac
     6721 gagcatcagt agcttccggg aaaacgattt tgatcccacg gcgaacaatt ttaaatctta
     6781 aactatcaaa caattccaca agatgacctc cagacgtttt tatatgtatt atcatacact
     6841 attccagaaa aaaagaacag tttattttat ctgtatcagt caaaaatgct ggaaaaatta
     6901 agtttttcac atacaaagcg aactgcacta taacaggaaa gcgctgtttt attacactgt
     6961 ttcaggcagt tgccaatcaa tcggttcttc tcctaacgca aggagcgcat cattcgtttt
     7021 cgagaagggt ttggaaccaa aaaagccacg acttgcagat aacggactgg gatgtggaga
     7081 tgttatgatg atatggcgac tcttatcgat cattttgatt ttttcttgtg ccggttttcc
     7141 ccacaagata aacacgattg gtttttcccg ttcatttaat ttttcaatga tcgtgtctgt
     7201 taatctttcc caacctttcc cgcgatgaga atacgcttgc ccttcccgaa cggtaagaac
     7261 ggtgttgagt aaaaggactc cctgtttagc ccatgacact agatttccat gttgaacagg
     7321 tgcgattcca agatcatcat aaagctcttt atagatattt cttaacgatg gcgggatctt
     7381 tacgccaggt tgcacagaaa aagataagcc atgagcttga ttttctccat gataaggatc
     7441 ttgtcctaaa atcaccactt taacttcttc ataaggagtc aattccaatg cttcatagat
     7501 atgatacata tctggatgga tcttttggga cgcatattct ttttttaaaa attctcttag
     7561 atgcagataa taatcttttg aaaattctga ggacaaaact tcttgccaac tgttatgaat
     7621 gatttctttc aaaaactcca cctcctaata aaaagaataa ctcacatttt cttcaatcac
     7681 aagggacaat cttcaccctc agcacttgat taatatggta aactaggaac aagaagcaga
     7741 aaggaacgat aatcattatg atcaaattaa ttgcatcaga tatggacggc acattattag
     7801 atgcccatat gagtgtttct cctgaaaatg ccgaggcgat acgttttgca aatgatgccg
     7861 gtgttgagtt catggtcgct actggtcgaa actatcaaga agctcgcgca gctttagatg
     7921 aggtaggaat cgattgtgcg atgatcactt tgaacggggc acaggtattt gataaagaag
     7981 gaaattcatt gtttaccgtg tcgattccaa ataatcaagc aatctctgtt ttagatattc
     8041 tagatgctaa cggtatttac tatgaagtag caaccaacga tggtctttat tctgaaaatc
     8101 aagcgaaacg aattgaaagt tttgcttcca tggtggctac ccatttacct caccttacgt
     8161 ataaaatggc gattgccatg gcctcagcta agctggagtt attgcacatc acatacgtgg
     8221 acagtattcg tgctattttg gaagaaaaga atctggaagt tttaaaaatt atttgttttc
     8281 atacagaagg gccagctgta ttaggtccag ttggaaagca gatttctctt ttaggcgatc
     8341 tagccgttac ttcctctggt caaaacaata tcgaagtgaa ccataaaaac gctcaaaaag
     8401 gaattgctgt tgcccatgtc gctcacgagc gtgggatttc tttagaagaa gtcatgacga
     8461 ttggcgataa cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt ttcgcaatgg
     8521 gaaatgcaga aatcgaagta aaagattatg ccaaatatac aacggacaca aaccttgaat
     8581 ctggcgtagg aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt tatctagaaa
     8641 agagggatat agatgtcaga atttttcatt caggaaaagc agcttggacg tgcaactcgg
     8701 acaattgtca aaaacgaaaa aggacattcc ctgtttttga tggttggtcg ctggggaaca
     8761 aagggcgatg cattgtctct ttatgccatg aatggcgatc tcgttgctca catcaagcag
     8821 ataagcctga cattcggtac gcgttttgag ctgtataaag agttcgaaaa ggtgggaacc
     8881 atgcagaaaa tattcaattg gcccggtgac ttttattata ttcgtagact tcactggaca
     8941 gtccaaggtg atatctataa tcatcaatat caaatccatc atttcaatca gcaaatcatg
     9001 aaaatggata aagcaacact tttttcagga gattactatg tattggatgt ctcaaatgat
     9061 gaagatgctc cagtttgtat ttgtattgct gcggttttag attactggct ctaccgtaaa
     9121 gacaaaagtc gcgataagcc tgtttcttac cgacttaact taagttaaaa agatgtacct
     9181 gaaatgcgga aatacgcatt tcaggtacat ctttttagat attcttttgc atatgatcat
     9241 aaagatgacc actgatcatc cgctgaccga caactttgaa gtcatggcgt tcgtataatt
     9301 ttttagcctt tggatttttt tcatccacac ttagaccgat gacggagcga ttagctttct
     9361 tcgccaattt aggtaatgct tctaataatc gcgaaccgat gccttgtcct ctaaaatctt
     9421 ctgatacact gatagagtcc aaataccatt cgttgggaaa cgtttcttta tcaatgaaca
     9481 aacgtgtaga cgcatctagc tgatatttct ctaagatagg taccagtggc tgatcgatga
     9541 ttgattcttc ttcatctgta taaccgtaag caactcctgc aactttaccg tctatttctt
     9601 ctacgatacc tcgtgaataa ctgtaacgat aatttggatc agtgattgct tcttctaaaa
     9661 tctctaaagt cttttgttcc cctgtctgct tcaaaaatgg cagttccata tcttttagaa
     9721 tcactaagat caatggggcg atttctggag catcttcttt tctcgcaaaa cgaatcatag
     9781 tttcctctcc ttttctccat tctaacatgc agaaaaagaa atcgcaaaat cgttatttta
     9841 aaaaatcttc aatcttttca gtaatgaaat aaccatgagt aacgattctt gccgcttcag
     9901 ttcggcaata agggcagacg actggcattt ctttatcatt atgttcgact acattattga
     9961 cttcttcgta tgcaaatttt tttccgcaat tagggcattt cattttttcc atgttatctc
    10021 aacttccttt catctttact ctcttagctt actcttatat tgtaaaaaag agaagcaata
    10081 tccttcttct atgcagatat ccatacctga cggtcctgtt ctccatctat taatcggact
    10141 ttataataag gacgttcatc tttgactaga agtgtccact ctttcacttt tgcttttttc
    10201 ccgtagtagc tgactgcttc cttcatcgct gcttgcggtt gtttgaactc ttccgttttt
    10261 tctatatcaa aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt tgttttccca
    10321 gtggatggat tgactgctat gacttgtcct tcacaaaaaa actggtattc atatgttgca
    10381 ttcgtaggat tctccaaatc tttttcaact gcaaaatgaa tagcagatat gttatgttct
    10441 tttgtttcgt tttttacgat tgctgctgct tttttcaatg agatccgata ttcctggtca
    10501 ctaatatcat ccatgtgaaa agcggcattg agcagtcccg tcataccggt aatccctatt
    10561 acgatgaggg cagcaacacc aaacatcatt tttttcattc ttcattctcc tgtttctttt
    10621 ttttaaagaa tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa aaaaagtgac
    10681 aattttgtta tctttattcg tgcagttcta atggcaatcc gtctggatca aagaaaaacg
    10741 tcattttttt tcctgtaaag gtatcttctc taatcggttc tgattcgatc cctaaggact
    10801 gcaattctgc aattgtttct tcaatatcgt ctactttgaa agcaagatga cgcaagccac
    10861 aagcttctgg atagtttggt cgttgtggcg cattagcaat tccaaaaatc tccaattcag
    10921 cacttcctag tttcagatct aatttatagt catctcgttc tttgcggtaa ttttctctga
    10981 ttacttcaaa tcctaattta ttaacataaa aatcacgtga tttctgataa tctgaaacaa
    11041 tgatggctat atgatggatc gttgtaaggt tcatatgagc actcctattc ttcgtttaag
    11101 ctcggatcta tattaatctc cagttccatt gtaaatgtgt cagattgaaa aaaatagctg
    11161 tttttccttt ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc caagacactc
    11221 ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa aacaaacaga
    11281 aaattatcat aacacaaatt atttataatt gaaagactag ttttttttca agaaaagcat
    11341 acaatagatt gtgttagtaa atacagaaca cactacattt agtgtcttaa ggagtcctca
    11401 tacatgaagt catattttca gtttttagct catcagctaa aaggctggcc gcaacaaaat
    11461 tattatcttt tctttttcag tcttggctgc caggtcatga ctcttgtcaa tagtcccatc
    11521 acttggctgt ccgttattac atttatcggt accacgcttg gtgtattgtg tattttatcc
    11581 atcaatgcgg caaaatcagt caacggtatt ttaggtattt tgtctgcgat ttgttttatt
    11641 atcgttgggt tttcagcaaa gaactattta agtatcggtg aacagattgc ttatatgatc
    11701 acattggata tccccgtatt gttaagtaaa gaatggaatc ataatatggc ttcaaaaatc
    11761 cgaaaattca ctggaaaaag ctggtttatc gcaatcatca gtactttcct tgtctatctt
    11821 gtttcaggat tcctgattgg tcgttataca gatgatcctc ggccgtgggt agatgccatt
    11881 gctttttcta ttagcttgac tgctggtatc atcagtttct tgcgttacaa caaccaatat
    11941 tactggtggt tagcttcagg gcttgctcaa atggtccttt ggtatatttc atttacgcat
    12001 ggatctgcaa gtttggcgat gttcgtgaac agctctgttt acttgctgaa tgatgtcctt
    12061 gcatttaccg tttcaccttg gtataaccaa aaagaacgtg aacgtatgca aaaaatggaa
    12121 gaagaatact acaacgcaca acaacacgca taaaaaaact gagcgtggga caaaagtaaa
    12181 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaatcagaag caactccttc
    12241 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
    12301 cttggagtta aacgcttctg tcccagcctc tatttcctac gattagtcaa gtaaaaggtg
    12361 caagaaaaat agctcgtaaa cgaaagcgac aaaatcaaag cacaccaaat aaagccccgt
    12421 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
    12481 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
    12541 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
    12601 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
    12661 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
    12721 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
    12781 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
    12841 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
    12901 tcggcttttt tgatcatttg tgtcgccagt gctttttttt gttcgagtaa atcttggaga
    12961 agttcggcgt aatagatcgt tttttgacag tggatactgt ctttttcaac agctggatag
    13021 gcacactgtg catagtggat gatttgatct gccttttgct cggcacgatt cgcggagatt
    13081 tttttccgtg tttcgttgat cagtttattt tttatttttg tccgagtaga tgccaataca
    13141 aaatctggat ggggaaaaag acggatcaat gtcaatgcgt aaggggtcag cctgtttgaa
    13201 aagaactgtt caagctctgg aaaagtcaat tgaaggcagt tgtgtaaatc catgcgcaga
    13261 cgagtgattt tcttctcaat ttcttgataa aaacgggcta aatcgcgcat ctctaagtaa
    13321 aaattgcttt gtacttcttt tggctgacgt tgatttttaa gatgagtctg tgcgagccgg
    13381 tgggcatccg atttatctgt tttccaactg cgtaaggtgc tttcttccat ctgttttttc
    13441 gcctctaacg ggtttaacaa acagtaagaa aagtgatgat cttgaaagaa cttttcgact
    13501 ggtctggaat aaatccccgt tgcttcaaaa acaatcaatg gacgctcagg caagtgctgg
    13561 atctcttcga gtaatgcatg aaatcctctt tgagtgtgtt caatctcgtc ttcaaataag
    13621 caggtgtcgt ctttgtatag gacactgtag ctgtgtccca ttgaaacatc taatgcaaga
    13681 atgtaaggca tttgttttcc tcctttttaa gttttgaaag aactttactg cttcatttcg
    13741 attccaattt cctatacacg ggctcatggc cccaacatac gtaaacctga ttctaaaatg
    13801 acagtaaaga agttcggttt ttgataggga ctcgtagtcc tcaaggcttt gttcgaactt
    13861 tctttcactc ttcactataa tctaaaaata gaaaatagag gagaacatca ttcgtcaatg
    13921 atgttcccct ctaatcttag tatgtttttt atagtaattc cttcagatac tttcttgcct
    13981 catcattgaa acgcagtgat tcttctaact tctgataata ttccaacgct tgtttctgag
    14041 cttcagtaaa acgataatgt tttcctttcc atttgtcgat tgtctcttgc acatttccag
    14101 attggatatc tttcactact agttgccatt tttcttttga gactgtacgg tctttcttat
    14161 cagcagattg tatagcttct cgttctttac gcaatacttt taaaaaacca tttctgacta
    14221 tccgaggaat gctgtcgaag aacccttctg taaaaccata gcgacaatat tgcttccaaa
    14281 taaatagata gatttttggt tggatgaatc gttggttttt cgcaaaatat tccatctgag
    14341 cagtgacttc tggtgttcgt tgacgttttg tctttctcaa cacggaaata aatgtttcaa
    14401 aataagcatt aggatgtact ggcacttctt catatacgat ggattgccaa tctttacgta
    14461 aaaatgcgcg atagttgatc aagaattctt ctacatcctt tactaattct tctgcaaagt
    14521 cgtcaaatag ttcttttttt atctgtcgct tttttgactg cccattgatg tgttcactta
    14581 ttttcttgat ttcattttcc aatgcttgat aatccgctat gtaaatcggc atttctactt
    14641 ggcgaaattc tgcgagccgc tgagctaatt ttatatccgg taaatatggc tgtttcatcc
    14701 gacgattttg gtacaagaca aaacagtatt tttgccatag tccttcagga aaatcgatat
    14761 tcttctgctt aagccataat aattcgtcta aggccagatc aacttttgtc attccctgat
    14821 ggatcgaagc tgccactgtc ggagagacat gcaggtgatc ggcaaagtgg ttgatgccta
    14881 gttttaggat ctttccggtt tttggcgatt taacgatgta ttgcactttt aattctctac
    14941 cacactcaca atacaatttt tgctggcttc gttttgttcg aaaaaacgga ttgatcttga
    15001 agtcagaaaa cacccaatct gttgcagcta aataatgttc ttccataaaa cgagagctga
    15061 ttagatatcg gcggtgctta tcgactagtt cccgctgttt tttcgttaat tttgcataga
    15121 tctcccaacg ttgactgctt gtgtattgtt tagaaatttt gatgggatag cttttgctga
    15181 tttcttttgg aaaccattct tcaaagacgg attgccattc ttccacggct gtttctttcc
    15241 tttctaatta caaaaagctg gctactaaca taacgttagc aaccagccag tgtttcttgc
    15301 taaattagtg agttgttccg gcatttccgg aaatatcttc tttattttct actgctgctt
    15361 caactgcata agtcaaagaa gaagcaatgt cttctaaaga catagaaggc gtacccactg
    15421 gacgatcgat cacttgatcg ggtgagaatg gcacgtggat gaacccacct cgaattgccg
    15481 ggaattgtgt ttcgatcaaa tgaagcaaac gatacatgat atcattgcag acaaatgttc
    15541 ctgctgtata agagatgtac gctggtagac catgatcttg tacatttttt gtcattgctt
    15601 tgattggcaa tgaggtaaag taagcggctg gaccgttttc ttctaaagcc gtatcgaatg
    15661 gctgatttcc ttcattatca gggattcttg cttctgctag attgattgcg acacgttcga
    15721 acgagactgc tgaccgccca cctgcttgtc cgacacagat cacgacatct ggttggtgct
    15781 gttggatggc ttctttgact acttgactgc ttttttcgaa tacggtcggt acttccactt
    15841 tgatgatttc agcaccgctg atttcatctg gcattttttt tactgcttca taggctggat
    15901 tcactttgtc cccaccaaat ggatcaaaac ctgttactag tactttcatc tgttgttcct
    15961 ccgtccgatt gatttaacat atggttatct tttatcatac cattttcctt gaacagcgta
    16021 aataaaggaa acaatagttt aaataacata acagatcaaa cttaatatat tcttataaaa
    16081 aaatagtgtt ccactacaat gttagaagag gttgcgacaa ttattctgtc acaacccctc
    16141 tcttctatta tttaaataga atcatataag caatttggaa aactagcata aataacgcaa
    16201 taaacaactg gtttttgatc actccgtatt tgtctttcat ctccagcatc gctacaggca
    16261 cgatattgaa gttagcagcc atcggtgtca gcagggtccc acaaaaaccg caagtcaatg
    16321 caaccattcc tattaacgct ggattcgctc catgactaaa tacgaatggt gcaccgatcc
    16381 caaccgtcat cacggtgatg gccgcaaatg cattccccat gattaccgta aatatcacca
    16441 tgccaagagc gtaaatgatg atgccgatgt tcacatttcc ttctggaacg atcgtaccta
    16501 ctgcattgga aatcactgtc ccaactccag cgcttgtaaa taccgcacct agagaagcta
    16561 aaagcattgg caacatgctt aacggcccta ctgtgcccag catatctgct gcatcatcta
    16621 aaaatgtact tggtcgatta tctcgagaaa agaacatcaa gattagcatg gctgcaaaaa
    16681 caccgacgcc cacgccgact aaagcaccta aattggtaaa tagagcaaac aagatcgcaa
    16741 agacaccgat actcaaagct ggaataaaaa ttttcattcc tagtttgtct gacatttttt
    16801 ccatatattc ttttgatgga agccggcttt ctcctttgct gactttctta gcaattgctg
    16861 gtatggtcat cgcaaagatc aagccaccgt tcacgattgc cggaagccat cttcctcctg
    16921 caataactac ccctaagacc gtccagaaaa aggctgaccc ataacgatga ggattggttt
    16981 tatctgttaa atttcttact ccagtgtaaa tcaatacgag tcccatcacg atataaagga
    17041 tctccagtaa tttatcgcct aataaaatat caggatttgt aaaaaagtcc atcttattgc
    17101 ccctttcctg gataatattt cttcgctaat ttcttttctt taagatagaa gtagacactt
    17161 gccacaacta aagccgtgat agccactgga acttccacta aagcaagctg tcccaatgtt
    17221 acgtcatatc ctagatcttt cagtgttgat tggactagca acgcccctgc tcctccgatg
    17281 aatagtactt ggccaaagaa ccagcagaca ttttccatag ctgaagccat accttttagt
    17341 tcttcttcat gcttaggatg gacttctaac ccttttgatt cgatggtccc caatgccatc
    17401 ggtaaaatga ttggacgaac aaatccagca actccaccaa aactcacgtt aaaagccgca
    17461 aagatcattc gaaagatccc ataaatatct acaatcatac cagcagatac tttcttgaat
    17521 cgcttgatca aggaagccgc tgattctttc agaccatttc tttcaagcgt tcctgttgcc
    17581 aacataatga tgataaaaat cgccatcccg cgattgttta caaaacttgt acctaaaagt
    17641 tctaacattc cttgaatgcc aagaccgcca gtcaaagctg taacaactaa agcaaccatt
    17701 acgatcaata tcgagtctaa tttaaatgca aacccgagaa taacaatcaa gacgcctaaa
    17761 aggctaagat aattttccat atattttcct ccataaaatg acaatatttt tttaattata
    17821 aagcaaaaca cataaaaaat atagttgttt tctcacgaaa tagacactat ttttaaatat
    17881 ttttcgttat ttttttatca caaaaattat cttacttatt gtaaggtaaa gcttttttta
    17941 atttattgcc cgataatata tagaaataag gtctagttac tgtttttttt aaatgattaa
    18001 taaataacaa cttctttcta tgaaattgca aagcattcat cttcttttcc gctaacgata
    18061 tatccgtcag tgcttggtac actttactaa aaagtatgag attgccgata tctggatagg
    18121 tgtgctatac tacagaaaaa gatatacaat ctgccaccgg gtaggataaa gaggatgatc
    18181 attccattag gtctttgaag ggatgatttt cctctttttg tgtatttttt tatagttaag
    18241 gaggaatgta agtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg
    18301 gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt
    18361 gattggcatg attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgccgatgag
    18421 tctctcttat cctatctgga cagggattgg cgcagttggt tcggtcataa ttggtgtggt
    18481 cttgtttcat gataagctga atctttccac ttggttcttt gtggggatgc tactcatcag
    18541 tatcattggc atcaagatta ctagcggcca ctagacactt ggaataagta ctgatcagaa
    18601 aaaataagcc tttttattta atattcaatt tttttatgaa aaaataagaa ataagtccaa
    18661 tgacctagcg acttctccac tcatcaggtg tatgcttgta ctatctatag aaaaggagtg
    18721 aatatttttg aaaagtcttt cggtccaaga attatccaag aactttggtc acaaacaagc
    18781 cctcagcgat gtcagtttta gcttatcccc tggcaaaatc gttggattag taggtcctaa
    18841 tggcgctgga aaaacaacga tcatgaaagc tattttagga ctgatccatt actcagatgg
    18901 aacaatctgt attggtgaaa atgagatttc tccctctagt cataaagggt tggaacaagt
    18961 aggagcattg atcgaatacc cgggtatcta tccattctta acaggctatg atcatctgaa
    19021 gttattctct gaaaccaacg atgtatctgc tatcgacact atcgtgaatc agctcaagat
    19081 ggaaaagtac attcacaaaa aagcgaaatc ctactcgttg ggaatgaaac aaaaattggg
    19141 aattgctctt gccttactga accatccgtc tatcgttatt ttagatgagc cgatgaacgg
    19201 tttggatcca caagcaacaa gagatgtgcg aaaaatcatt accgacttgg ctactcaggg
    19261 cacctctttc ttgatctcca gtcacttact cagcgagctt gaaaaaatgg tcgatgagct
    19321 gatcctgatc aatcaaggaa agatcatcaa acaatgtaca atggctgaat tgaaccaaac
    19381 agcgcaagat ttcatcgtta tctctacaac aaatgatcta gctgctcgaa cgatcttgac
    19441 agaagctgga tatactttgg aagatcctga taaaatcaag cttttaaaag aaaacgaaga
    19501 attattagga accgtgatcc agctattgtc tgcacaaaat atccatgtga cagacgtcca
    19561 gcatctgacg aatgatttgg aatcttctct attggaaatt ctggattcag taaaggggga
    19621 agaagaaaaa tgacattgct tgtaaaacaa gaattattta aattgatcaa gaaaaagtca
    19681 actgctgttt tatctgttct attagttgtt ttgctcatag gaacagcact tctagccaaa
    19741 aaatacacga cgatcattga tccagttgaa atgactgctc agctttttag tgccacatct
    19801 tggattgtct ttatcatgat tgcagctgca agcacgatca tttcgatgga agcacaatat
    19861 ggtacattga aaaatctcct ttatcgaaag tattctcgag gagaaatatt agtaagcaaa
    19921 tggatcacat tagttattta ttctgtttat ttataccttt tagctattat tgtcactgtg
    19981 ttgatgaaac tgattctttt cccttcgatt tccttcacgg agaaggtttc tactggtcaa
    20041 acactgatcc aaagcttgtt cacttatact ttgggtagtt atatcggttt atggctgatt
    20101 ttaagtttag tgttgatgat tgcttgcttt atcaatagtt ccggcgcatc catctcagca
    20161 ggaatcgtct tttattttgc ttcgtcaatc atatctggta tccttattgc actcatccaa
    20221 aaatgggagt ggatcaaatg gaatccgatc agtatgttga atctgcaaaa ccaaattgga
    20281 aacgaagaga tcatgaagca attgactcat ctttctacta atcaaatgct attcggtaat
    20341 ttagcttaca tcgttttgtt cttagcatta ggatatctcg tatttaagcg aaaaaatgta
    20401 tgatgactca tcaaaaaaga ggctgtgaaa gagacgttca gccttattgc cgattggctg
    20461 tctcttaaac accgtttatt ctgaaaaagg agctgtgaca acatttgtgt cacagctcct
    20521 ttcctagtat gtttttgaat aaacgacagg ctcacaacct ctctttatac ttcttttata
    20581 cttctatacg tgaagcatcc cagtggcaaa taaaaccatt gcaatcactc cgaccatcgt
    20641 cacacctata agtaaaatct tatctaagtg gatatgttgt acaggctcgt tttgctcctt
    20701 acgtgctttg aggtaaaaat aaatgcctgg caagaaaaga acaagaacaa gcattacttc
    20761 attaaaggct acgactagca tagcccatac ttgaaaggca cttgctaaaa gccctactaa
    20821 aaattggacc cattctttcc gctgataaga aagtttcatt tggtacaatc ctaagaaaag
    20881 ccacgtgaag aaaatagctg ctgtaccaag tgtgtaagca aagttataag cactgtcagt
    20941 gaataataat gaaagtaaaa agacattcgt acataacgtc gtcaatacga tagcataagt
    21001 tggtgcccca tttttgttga gttttcccca tgctttaggc aaaagtccgt cttcagccat
    21061 gtttcccatt gtttcaatcg gtaagatcgt ccatgagagc caaacaccaa ctaaagaaat
    21121 gatcaaaccg ccgttgatca cgactgctcc ccatttacca accatcattt caaacacata
    21181 gcccatagca ggctgcgcct tgatcgtttg cagctgattc atcgtcattg tcccatatgg
    21241 aagcatggaa accaagacgt aaataataat caagccgaat atccctagaa tcgttgcttt
    21301 cccaacatct gattgttttt ttgctcgtga acctacgaca gaggctcctt caataccaac
    21361 aaatacccaa agcatcacca tgatgcaact caatacttga ctggaaaacg ggatcggctg
    21421 tccattcgca ttcaatataa tgtcgctaga aaagttatca atgaattgtt ggatcgaaaa
    21481 cgtgctgata gctactacaa taaataaaaa taatggcact aatttacaga atgtgacgat
    21541 agcattgacg aaactagcat tttcgactcc ccgattcact agccatgcat atccccataa
    21601 aaagactgta ctcatcacga tcgatgccag attttgccca ccttcaaata ctgggaagaa
    21661 atatcctaat gcgctcatca acaatgttgc aaaagctacg ttgcctaacc agcatgtgag
    21721 ccagtaaccc caaccgctga taaaaccagc gaaacggcca aaaccttctt ctgcgtaact
    21781 aaagatcccg ccttttaaat ctggacgttt tttattgata ttattgatcg acaaggagag
    21841 catcaacatt gctgttccta cgagtaccca actgaaaata gctgggccgc cagccgatcc
    21901 ggagatgtcc gtcataatac caaatacgcc acctccaatc gctgaaccaa ttactaaggc
    21961 agcaagtgga attaaaccaa ttcctttttt ctgttgctct gtatccatgt aagcatcctc
    22021 caaaataaaa ccgcttttat caaaaagtat aacaaacctt ttttattttt acttaattaa
    22081 taaaaacttt aatagaaata taattctacc ggcttttttc tcattataca agcaaagcgc
    22141 ctagtccctt tccaaattac accaccaaaa aacttaaaaa agatgctata ctaaaaggaa
    22201 agaattggag gatgatccat gaacatttta tatatatcga tttcaggaaa tacgcgtgcc
    22261 tttgccaaac atctagcaga atatgctgaa aaaatgcatg cagaagatcc tgtcaatcct
    22321 gaagttactt taaaagaaat ccatgaaaat agtgatttta cgaaagaaac agaaccattt
    22381 tttactttcg ttcctactta tttagacggc ggaaatggtt tagataacgg tgatacagaa
    22441 atcttgaccg aaacgatgcg tgaatatctg gaacatgaag acaatcataa actttgtctt
    22501 ggtgtagtcg gtagcggaaa taaaaacttc aataatcagt attgtctaac agccaaacaa
    22561 tatgcacaaa catttggctt tcctttcttg gcagattatg aattacgggg aacaccatct
    22621 gatgtgacac gaatttatca gattttagca gaaaacaata aataataaaa aagaagaagc
    22681 tgaaaaatag atttcagttt cttctttcgt ttctattgtt tctatcatat taagctttga
    22741 catccatcac aggtgctttt gcttttgctt tacctgtttt ttccaagaca aagtttttta
    22801 cacgatccat attggttacc aagacgatca tggatgtttc tttgcctgct gctttgatcg
    22861 cttctaagtc agcttctgct acaagcgtat caggtgtcac ttgctgccct tctgttactt
    22921 tcacatcaaa tggcgtaccg cctaagtcaa ctgtgttgat tcccatatga agcaagattt
    22981 ctaaaccatt tggcgttgtg atcccaatag cgtgtttcgt tgggaagaca gagctgacta
    23041 ccccagtaac tggagaatag atatgtccgt tttctggttc tacaaagtag ccttcaccca
    23101 tcatcttttg tgcaaaaact ggatcacttg ctttatctaa agaaacaact gttccgtcag
    23161 caactgatga gaggatagtc acactttcag ctgaagtttg aggtactact ttttcaggca
    23221 cttcatttac tgtggctgtt tcagtcgcta attcttcttt ttctaaacgg tctttaggaa
    23281 taccgaagaa gaatgtagca acgaagccac cagcataagc tgccaataat ccaaggatgt
    23341 agcctaacca tttaccgtca gagatcaatg ggatcaaagc ggctcctgaa ggtccaatag
    23401 cgatagcccc gacatttccg atcattccga tcactgcccc gccgatacct ccaccgatac
    23461 aagcagtaat aaatgggcgt cctagaggta atgttactcc gtaaatcaat ggttcgccga
    23521 ttcctaggat cccgactggt aaagctcctt tgatcatttc tgttaattct ttatcttttt
    23581 tgcagcgaat ccataatgcc aatgctgcac ccacttgtcc tgcaccagcc atcgcaagaa
    23641 ttggaagcaa tagcgtacga ccagtttcat caatcatttg cacatggatt ggagtcaaaa
    23701 tttgatgcag tccgaacata accatcggca agaataatgt gcctagaacg aagccggcaa
    23761 atgctccacc aacgccaagt acccagttga tcccaccgac catgccatca gaaatgaaac
    23821 ctgctaaagg catgatgaag aaaatttcaa tcagaccgat ggcaatcaat gaaagagttg
    23881 gtgtaacgat gatatctacc gcatcaggta ctactttatg cagttttttc tcaacgattg
    23941 acaatagcca aacagcgaag atgacaccta ggatcccacc ttgtccagct gcaagagctt
    24001 caccagtaaa taagtttttc agtggtgctt ccggattcat ccctgtcagc atcacaacgg
    24061 ccccgataac tccaccaaga gttggtgttg caccaaaaac ttgtgcagca ttgatacctg
    24121 tatagataac taggtagcta aacatcccgt ttttcaagat gttcatcaca tcgatatatt
    24181 gttgccatgt tgctgcatca agtgttccgg ctgtgactaa gtttgctaaa actgcggcaa
    24241 tccctgccac gatcccagta ccgacaaatg caggaatcat tggcacaaag atattagaaa
    24301 tgtcttttaa taccgatttg attttagatg gtttttgttt cgatttttgt gccgctttca
    24361 tttcttgtgc ccgttcgttt acacggtctt ttccagatgc tgatgaattt gctgtagctg
    24421 tacttacaga agaaagtttt tctcctaatt tcacgcctgc caaatcaacc atttgctgtg
    24481 ctactttgtt cactttccca ggtccgatga tgacttgaag ctgttcatct tctactacac
    24541 caagcactcc tggaatcgct tttaattcag agacatttac aagatcatca ttgatgatct
    24601 tcatacgcac acgagtcatg cagtggacaa tgctttctac attgcccatg ccgcctgccc
    24661 ctttataaat ttgttttgcc agttcttcca aagtcagttc gttttttgcc atggtctttt
    24721 ccccctcata aggtcccttt tacaaatccg tttgcttcag ctaattttgc ttgagcttcc
    24781 tctttatcac tatttgtcaa aatcatgacg atagccagct taacattttg atctgcttct
    24841 tcaaagtact tttcagctgt ttcgtaatca gcttctgtcg cttgcatgat gattcgtttt
    24901 gagcgttcga ttagtttttc atttgttggt ttgacatcga ccatcaagtt attgaatact
    24961 ttacctatac caatcatact gatagtagaa atcatattca agataagttt ttgtgctgtt
    25021 cctgacttca atcgagtaga gcctgtcaaa aattctggac ctgcatcgac ttcgattggg
    25081 aaatccgcgt gtttactgat ttcggcttgt ttattgcaag aaatcgttcc tgtgacagca
    25141 ccgatcgatt ttgcgtaatc taatccaccg atcacatatg gtgtacgacc gctagctgcg
    25201 ataccaataa ctatatcatt ttttgtcaaa ttcagcttga ttagatcttc ttgccctagc
    25261 tcataagaat cttcggcccc ttcgacagct acagtcattg cttcttctcc accagcaatt
    25321 agacctatga ccatttccgg ttctacaccg aatgtcggta cgcattcagc agcatctaac
    25381 acacctagtc ttccacttgt tccggctccc atataaatca gacgtccacc ttttttaaag
    25441 gactcaatcg tgttagcaat cactggttcg atacttgtca gttctttttc aaccgcagcg
    25501 gccactttct gatcttcttt gttcattagt tgaacggctt gagaaatact catctcatct
    25561 aatccaaaag tttcttggtt tctgcgttcc gttgttaggt tctctaaatt gattttgctc
    25621 atttttatct tccttctttt cttataaatt gatagttcat accagctttg atttggttga
    25681 tcaatggtag ctcttctttg ataactttag ctactacatt cactttttca tccgctggca
    25741 aatcatattt ggttaattgg atctcaccca tatagcgcaa atacttttcg ttatctaaag
    25801 taatagaccc tttcatacgc gtagctgtat ttcttgctgg gataggcgga atctctcgaa
    25861 aacgtgcatc tacgcttcgt atgacatctc ttgcatcgtc ttgccgattc gtatgcttcc
    25921 ctaatatata ttcgtaaaat tcttcatcta caggttccgt atggagcaag actgtctgtt
    25981 ctttttggaa agaagcaaat tgctcctgaa cgttttctga cagacctgca tctccgatgt
    26041 aaacaatatc cgtgttactt gctaatagat ctagagcgcc tgcaagagga tggatccctc
    26101 gatgttcttc caaagttggc aatccttgat atagtgggcc tctcaactct gtatctcctg
    26161 gtacaaaacc ttggacaagg agaccttgaa ttttgagcca ttgatttttt ttctgatacc
    26221 attctttgtc cagccctgtt tcgggacgtg gataataatt atgccaagct tcaatctgtg
    26281 agaaatcagc ttctgcttct ttcaattcat cgatatcttt aggcgtgatc gtgctagcat
    26341 tcaaactgat tttcaattga tgtgaccagt cggcaatctg ctgattggaa atatgataat
    26401 ccattcttaa acctgttaca ccaattgctt tcaaaggcgt tagatcttct aatgaaaaac
    26461 ccgctcgttc caatgcctct cctgaaatat cgaccatcaa ttctagttta ttctttttcg
    26521 cttcgcttcc tagttcgatc aatcgctttt gatatgccga aacatcatct tctggtatat
    26581 gcatggaagt gaaaataccg acaaagccat tggatgccat ttggcggata tactgtttct
    26641 tctcttctga caaagaatcg ttcataaata ttgaaaagcc aaacatatta tccctctttt
    26701 tctgtatccg tcttcatttc tatgactaga gtatatatct aaagaaacaa aatttcaata
    26761 cttttattaa aataattaaa tttcatttct aaaggttgag tttatattat caaaagaatt
    26821 gtcataacct aaaaaaggca ctgctaagca aaagcagtgc cttgaaatat tttatttaaa
    26881 taacacaaaa acataattac tcataagaaa ccaattctgt attatctacc atgaatgtaa
    26941 agaacccctc attaggcatc ggatgaaaat aagcattgat cactcttatc aaggcattat
    27001 ggcaaactaa cagatagaca ttttcttcgt cttcgatgca ttctttcaat aaaggaacga
    27061 tccgtgcata cacatctaag actgactccc catttgggaa tcgtacagcg aacgctaaac
    27121 gtgctttttg aaagttctca tctttgcttg gcagtccatc ataatcccca aaatccattt
    27181 ctaccaatcg ttcatcaacg gtcagcggca agctgagacg atcagcaact gcttgtgctg
    27241 tatctcttgc tcgttgaaga ggggaatgga tgattttcgt gattggaaca ggcaaatcta
    27301 ctaccttttc tgctagtttc tgtgcctgca tctgtccttt ttcagtcagc ggcacatctg
    27361 cccgtccgca aactaatcct tgtgcgttcc aagttgtttc tccgtgtctc gttacataaa
    27421 gcatgtgatt ctctccttcg ttggcaaagt tccccttaac cataccttgt tgttcttgag
    27481 attacaaggg ttagattctc tcaagagaaa taatctcagt agatacttta tccaggaagg
    27541 tttgatcatg ttccactagt aacatggcag gtttcacgtt caagatcagt tgttctaatt
    27601 gttcttgatt gaagacatcc aaataattca atggttcatc ccatgtatat agttcagctg
    27661 gctgtgataa agatttagcc aattccactt ttttccgttg gcccatactc atctgctcga
    27721 tcttgttatg aaaaacatct ctttccatcc caagctttcg gaggttgttc aaaaatgctt
    27781 ggtagtctac ttggtttttc tctgcaaatt ccgccaacgt tcctcgattg tcttcataat
    27841 tttggcttgc ataactaatg ctcagatgtt ttggctggta tttttctcct ataaccttgc
    27901 cgttgaatgc ccccagaaga taatggataa tggatgactt tcctgcaccg ttaggacctg
    27961 aaatcgccac ccgctgatga ggctcgattg taaaatgaat tggctcgaat aacagatttt
    28021 cataccctaa ttgaagatct tctacgctta aaagtcgctt atggtgagac gcttggctat
    28081 tcatcgtcaa agaatcgata tattcgatat cttttaatag tttttctttc tcgctgatct
    28141 gggtctccat ccgattcacg attgctttgg atcgtttcat cgtccgtgca gcatcagcac
    28201 ccactgctcc tttattcact cgtctagatt cagtatcgat gaatccgact tgtttctttg
    28261 ttttatctcc ttctcgggaa cgagaccatt cggctttttc agctgctgtt ttctttagcc
    28321 tactgacttc ttttttcaat ttttcatttt gagccatttc aaattcatca cgaagttttt
    28381 tctgttcttc atagatagag aaattccctt gataaagttc cagttgactt ttttcgattg
    28441 ctaaaacatg gtccactact tcatcgataa atccccggtc atggctgacc acgatgaagc
    28501 cttgtttttt ctttttcaaa taagccgcta cttgttttct accagagata tccaaatgat
    28561 tcgttggttc atcgattaac gggaaatgag tgtcatccac aaataaaagt gccagtagga
    28621 cttttgtctt ctctcccccc gatagtgtgc tgaattctct ccataagatt tcaggatctg
    28681 tttgcatcaa ttggagctct ctttcgattt cccatatctc aaaatccgta atatcattta
    28741 acacgtaata ggttaaacgt tctttatctt ttgtctgttg cgggaaatag gcaaattctt
    28801 gctgatggat tacttgccct tgataaggta gtttgttttg tagaatattc agtaaagttg
    28861 tctttcctcg accgtttcgt ccgataagtc ctagtttcca ttgtgtgtca aaatttagat
    28921 ttgcttgttc aaataataat gtgccttggc tgtcgtagcc gaatgtcaga ttttttattt
    28981 cgattttaga cattttccat cacctcgtaa aaaaagacca gtcctatttt cagaactggc
    29041 taaagaggat gcaagggttg gatacacaca aaaagaccca tacatcgaaa aaaccaagcc
    29101 aatcccgaaa atctgcacac tgcttccttg tctccacaag gtacagtctg aatgcagctt
    29161 tcatcaatcg acttaatttt tcaatgcacg gatccctcac tttgtcctat atttgaaatt
    29221 acgatagcat aaagaaaatg attttgtcaa ctacttcggg aggatggtga aaaatctgct
    29281 aagtgtctgt cctaagattt ttgttttttc taatgtttgc ctgtgttcca ttgcttcttc
    29341 taaacctatt ggacccaaat gaatacttaa gacaattggg agaaaatcag aaagtccttc
    29401 tagctcttgg tctctacttc cgcaaatagc gattattggt tttcgatatt tttttgcaag
    29461 cactgctaca ccgtatggaa cttttccgtg gatcgtttgc tgatcgatcc gcccttctcc
    29521 tgtaatgatg acatctgctt gttcgatctt tttttccaat ccagtgattt ggctgactaa
    29581 atcgaaacca gaaaccatct tcgctcccaa caaaaaaagg gctccaccaa taccgcctgc
    29641 tgctccactt cctggaatat catctaaatc cacctcccag aaatctttta tttgctgagc
    29701 aatctttcct gcttgtttat ctaagaaagc tagcgtcgta tctattcctc ctttttgtct
    29761 tccaaattgc caagcagctc cttgttgacc tgtatatgga ttggtaacat ctgcagcgat
    29821 aatcaatttt actttagaaa caagaacgtt tcttcgatca aaatgaagtt ttttagtaga
    29881 taataaagga tttccttctg tgtacccttc taaagaagca cccaaggatt ggagtaatcc
    29941 tagaccacca tcgctacacc cgcttcctcc taaagtgaca ataatttgtt ctacttcttg
    30001 aagcagtgca tctttaataa gttctcctaa ggcataactg gaagcaagtc ggatagtttg
    30061 atccgttggt ttatcaagta gatctaaccc caatgcttgt gcagattcga tgactgcggt
    30121 gttttttcct tgccaagttg tcaataaata gctgattttt cgctgacgga ataagagatc
    30181 catcccatca atggtacgcc atgttcccct gagcgcgtaa tagattgctt ctgcagtatt
    30241 ttctccgcca tctgcaatcg gcacattgat ttttcttcct ttacgatctt cccagccatt
    30301 tagaactgcc tgatttagtt caaatgaaga agcacttcct ttaaatgaat caattgccgc
    30361 caaaatatcc atctgtatcc ctcactttct tcttaagtaa gacacttttt ttcatttttt
    30421 tcaaacaaaa caatgctatt ttcttccatt tttctatata ctaaaaggga actcattgaa
    30481 taaaggagag aaaaaacatg aaaatcgcaa tagctggggc cggagcaatg ggcagccgaa
    30541 tcggattgat gctacatcaa agcggaaatg aggtcctctt gatcgatcgt tggcctgctc
    30601 acattgaagc cattcgtaca aatgggttga ttgctgactt taacggtaaa gaagtggtcg
    30661 caaaactgcc aatctattca ccagaagaaa tcatcgaaag caacgaacac gtggacttga
    30721 tcgtagcatt aacaaaagcc aatcaattag atgatatgtt ttgttcgatc caatccatta
    30781 ttactgacaa tacttatgtt ttgtgtttac taaatggatt aggtcatgaa gatgtactgg
    30841 aaaaatatgt gccaaagaaa aatatcttat ttgggatcac gatgtggaca gctggtctag
    30901 ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat cgaactagag aacttggaac
    30961 cagaaggcga agcatttaca aaaaaagttg tagaagtctt tcaagaagca aatttaaatc
    31021 cgatctatag ccataacgtt cgttattcca tttggcgaaa agcttgtgta aatggaacat
    31081 tgaatggttt atgcacgatt ttggattgta atattgctga actaggcgcg caaaaagcgg
    31141 cagagagcat ggttcgaaca atcgtatctg aatttgcttc tatcgcagca aaagaaggaa
    31201 tcatactaga tcaagaagaa gtgtaccaac atattgcttc tacttatgat cctgacaaca
    31261 tcgggttgca ttacccatct atgtaccaag acctgatcaa aaaccatcgt ttgactgaaa
    31321 tcgactacat caatggtgcc atttggcgaa aaggacaaaa atatgatatt gctacaccat
    31381 attgtgcctt tttgacccaa ttagtccatg caaaagaagg aatccttggc gcagaataaa
    31441 aggctctctg atgagaatca gctgaatcag cagctggttc tcttttttta agcccaataa
    31501 aagatcaaaa agaaaacaca actctcatga tgccataaga attgtgcttg tttgtttcag
    31561 tacataatct cttcaaaatg taggtttttt atttgttttc aacagaggag aggtcatatt
    31621 cgtattgctc ataaatagtg gaatcgttag tgataaatcg ttttgcccaa ttttctgcat
    31681 cataaaattc caatagctct gataggattg gcttaaatgg tgtatgcaga aattccggta
    31741 attcatctaa atcacgttct cgaagcaaaa aaatattttc aggacgataa aaatgataat
    31801 gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa atacatggat tcaaatgtcc
    31861 gcaaagtcac tccttgctga ccctcccgca acagttccag aatcccgcgc gattcccctg
    31921 tttttttcag gtactcttca taagtaagat atccaggttg ttcattccct cgatgtggaa
    31981 gaatgaaata gttggtcgta acatctcttt ttataaattc ctgcttatag ctttctgctc
    32041 gttcttttct cccattattc gttgcaccaa aaaatagatc cgtctttggt tcgctagaag
    32101 aaaaattcat atgtttcgaa taaaatgaac tattatgttt cagcccaagt tcttttgctt
    32161 ccaaaaaatc aaaatgataa atctcatcga ttgccggttc atcttgtgcc tgcttcagca
    32221 tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa cacaattttt gcattggtaa
    32281 atgggcgcac gcgtcgagcc atctccatat caaatccata atctaaaaaa acgatcagct
    32341 gataatttgc caactgttgt ctagctgatt taatgaattc cgactgtagt aaatcttttc
    32401 cggaccaaac atctaaagag cggatggaag gcaaaaaata aagtgattta ttttttgcaa
    32461 ctagcagtac gtcatttttg ttcatctttt ccgcctcact ttctctgtca ctttttccta
    32521 tgatagcctg tttaaatagt aaaaagccag taaaacgttt cctatatttg tatgaaaatt
    32581 taacatttgt atgaaaatat gatgaaataa atcattcttc tattaaaatt cgcctaaaag
    32641 ttttctgaat agttgtaatt gtctgaaaat ttgttatcat tagtcattgt taaatattgc
    32701 tatttattct taatataaat tttgattgtg aatttaaaag aaagaggtca tttatggaag
    32761 ataaaaaatt gaaaaaacaa cttagctcca gacatatcac catgttagct ttaggtggag
    32821 caatcggtgc aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg
    32881 tattgattgc ttttttgatt ggtgggctga ttttatatat cgtgatgaaa ggtctgggaa
    32941 aaatcgtttt aagcggtggc gacacgcatc acggattatc tggtttgata cgcccttatc
    33001 taggtgctca ttctgctgat tttactgatt gggtctactg gtctatgtgg attatcaata
    33061 tcatcgctga agctgttgca gctgcttcct ttttgcagtt gtggttcccg cacattccag
    33121 cctgggtatt tgtttttatg ttggctgttt taactacgat catcaatctt tattcggtac
    33181 ggttattcgc agaaacagaa tattggctgg cttttgctaa aatcagtgtc attatcttac
    33241 tgatcatttt tgctacttat cttgtcggtc agcaaatgtt gggcacaggc gttttcccta
    33301 cccttcagca attgactgac catggtgggt ttactcctca tggtatgaag ggaatcatca
    33361 gttctttgct tgtagttatc tattcttacg gcggttctga actgattgct atcacggtaa
    33421 gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat caaaggtgtc atgggaagaa
    33481 tcatttcctt ttacatcatt cctttgtttt tgcttttgat tctttttcct tggaatactt
    33541 tagcaggcac gaatgtcagt ccttttgtga tggtatttga aaaaatgaat atcccatttg
    33601 cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt ctcatctatc aattcgggtg
    33661 tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa
    33721 aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgttttattt tgtaccagtg
    33781 ttctttatct tggtgtagct ctttcttatt ttgtcggtga caaattattt ggctatctgg
    33841 caggttccct ttcgtataca gtattattga tctggatcat tatcagtgcg gcatcctttg
    33901 tactggcact aagaaaagga acattatggg ataaaggaat cagctttttt gcattagctg
    33961 tcttaggatt gattttcctt ggtatcctgt tcacgaactc cattggggta actgtattga
    34021 ctggcctgct ttacctgttc atttatttca gttatcaaaa aaagaatgat gcgttcgtat
    34081 tgacgaatga atgaaatcga tagaaaattt tattttattt atgtggaata gatggagcta
    34141 ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa taaacagtct
    34201 catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg attgttctcc
    34261 atgctcatga catgatagaa gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag
    34321 tattggaagt ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac
    34381 ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta cacgagtatt catggcgttg
    34441 atgatgggtg cagtcatggc agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac
    34501 aaaaagataa atgttatgat cttagccgct agtcttcttg tttttgccgg ttccttgttt
    34561 atggtacgaa gccaaactac tgtcaacgac acagcttgga tgaaggccat gattcctcat
    34621 cattctatcg ctatcctggc aagtaaacga gctgatttaa aagatcctga agtaaaaaaa
    34681 ttagcggaag aaatcattca ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc
    34741 aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag aggatgttcc agaagttatc
    34801 agcttcaaga aataagaaga agctacttct ggaacgccgt gaataagggg ttgtgaaaaa
    34861 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcct atttttcgtt
    34921 tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattaag aggttgtaaa
    34981 atcagcgttc tgacctgagt attagagaga caaatggaaa aatagttcct catattttgc
    35041 cattttttga gctaataccg agggtcagct gattgaacac cgtttatcga gaggttgtga
    35101 aaaagtcgct tagtttcgcg tattaagaaa aattttgtga aatggctttc cacatttttg
    35161 tataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttattc gtaaaaggga
    35221 ttgtgacaaa agtcttgtca caatcccttt ttatgttttt attgttctaa ttgcgctgtc
    35281 ttgcttactg cttcttgcgg tttcacaaat ttttttgaga ttccccaacc tgtgaaggca
    35341 ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata cgaatggcaa gtaagtgagt
    35401 gtaggaatac caaatgtact tgctacgaaa cttccggcaa ttccccaagg cactaagtag
    35461 ttgattactg ttccaccatc ttctagcgtt ctacctaata cagttggatc aaatcctgct
    35521 ttttcataag tttctttaaa tgcattacca ggcagaataa cagataggaa ttgttcacca
    35581 ataaaaatat ttactccgat acttgtcatc aatgttgtaa aaatcaaatt tggtgtgttc
    35641 gtcatctttc ttgatactcg ttccatcacg gttgagatca agccaaattc gatcaacaat
    35701 ccacctaatg acaaagttag aataatcaat gacacagtag gcatcatact ttcgattcct
    35761 ccgcggctaa gcagtgaatc gatttgttgg tttccagtat ttgaaacaaa ccctgattcg
    35821 atgaccccag ccaacttcgt taaggaagtt tgtgggtttt gaataaaaat cattcctact
    35881 gctacagcga tattcaacaa aatcgttaaa attgccggca cctttttcca tgcacacagt
    35941 aacattaaaa ataaaggaat cagtgaccag attgaaatag taaagtttgc ttctagaaca
    36001 tcagtcactt cacgaataga tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg
    36061 aataaaaaga aagaaacaac aaatgccggg acagttgacc acatcatatg cttgatgtgt
    36121 ttgaacaaat cggcatcaac aatagctgct gctagatttg ttgattcgga taatggagac
    36181 atcttatctc caaaaacggc acctgaaaca atagctccaa caaccagcgc tggatttaag
    36241 cctagtgttg tcccaatacc gaaaaatgca atcccaatcg ttgacataac agtaaaggca
    36301 ctccctacag cgctacctac gattgcacac acaacaaata cagaagggac aaaccactgg
    36361 acactgatca atttgaaccc aatgaccatt agagttggaa tgatccctgc ttgtatccat
    36421 acagcaatca atgaaccgac taaaataaaa ataaagattg ggatgattcc tggtttgatg
    36481 ccattgatga ttccttcgtg cagccaatct gtcgggtatc tttttatcac tgcatacaac
    36541 atgaccattc cgatcgctgc taagatcgtg atattaggtg agacctttaa tccgacaacg
    36601 ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg ttgcttctaa aaaacttggt
    36661 gttccttttt ttcccatgat aatctctcct tttttagttg ttcttatgtt ctaaaaaagt
    36721 taagatatcg gattactccg ccgcaattca tccgcgattc ctcctttata aaaataaaaa
    36781 gacctgtttt aaactaagga cgaatgactc cgcggtacca ccttaattaa aaacagatct
    36841 gtttttcact ttcaaacttt tattcaaatg tatggacttt ccatgtaatt cgtcaattag
    36901 gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa acacctaacc gctactgcgt
    36961 gaaaagttga cttgtacgaa gtttaccttc cataagatca tttgtcaatg aaaaagaaaa
    37021 tattttttca tttttctgaa aattattttt ataactttca cacacaagat tattttttac
    37081 tagaaagtag cgccctcttg tttttttctt gctactattt cttgatgttc ttgcttgatt
    37141 ctttctaata gctttggctc tttcaacaga tcaagagctg tattggcgag tagttctgct
    37201 gcaatcgcaa tagaagcaag gcctttgtca cttttagcag cagctttgaa ttcttctgtg
    37261 tgtccggcaa tatatgtctc agagattgct accgtaggct ggattgttgg tatgacttgg
    37321 ctgacatttc ctacatcaga agaaccgaga cttgttcttt gttccgtttc tatttcttca
    37381 tctggaacac ctgcttcttt tgcatgctgg aagaataagt catcaaaaga aggagtaacg
    37441 atgacatcat caactgcatt ttggaacaat ccaaactcaa aagtcgtgcc cgtacttatc
    37501 gcagcacctc gtacgatatt ttccactttt tcatatactt tatctaatgt ttgccgattt
    37561 cctgcacgta aatagaaacg tccagcagcg taatctggaa tgacgttggc agctactcct
    37621 ccgtctgtaa tgatcccatg gatattgaca tcttttggca attgcaagcg tagagcgttg
    37681 attccattaa acacttggat caatgcttcc aaagcattta ctcctttttc aggtgctgct
    37741 gctgcatggg aagcgacacc gtaaaatttg atgtcgactg gatcattcgc taaagattct
    37801 gtcgttttcc cataacgaaa agctggatgg acgcataacg ctacatcgac atcctcaaaa
    37861 aaaccttctc gaacaaagct gcctttagct gatccgttct cgcccccttc ttcccctggg
    37921 gtgccatata cacggatttc tccaccagtt tcatcaatga attgcttcac tatgctggcg
    37981 gctaatacgg agtaagtacc aaacaaatta tgcccgcatg catgaccaat acctggtaaa
    38041 gcatcgaatt ctgccaaaaa ggcaattgtg ggaccgggtt tattggaaat ataacgtgca
    38101 tcaaaggctg ttcggtgtcc agctacttct ttttctaccc aaaacctttc tttttctaat
    38161 tgtttaatca acgctgctga ggaataaaac tcatagttac ttacttcagg atgttcgtgg
    38221 atatctagag caagtgcttg atattctggt aaacgatatt ggattagatc cttcaattgg
    38281 tttgttgaga ttgttgttgt cataaaaaat tcctcctatg ctagtcattt ttcttatttt
    38341 ctcccgaatg tttcccatgc aggcacgctt gatccttttg atgttttctc gattactttg
    38401 actgtgtctt ctgtttgata agcgtctact attttttgat acgttttatt gttttcatct
    38461 tcttttcttg caacgatgat attcacataa ggacgtgcat ggtcatccac tggttctaag
    38521 aagatggcat cagaagcagg attaaatcca gcatctacag ctacaccgct attgatcact
    38581 gaaatgtcta catctgacaa agcacgtgct gtttgggaag catctaattc agaaatgtct
    38641 agattttttc tattctcggt aatatcagaa acagtgggcg cttgtccttt tgccttatcc
    38701 actttgatca atccagcagt ttgcaataac agaagagctc tgccgccatt tgtaacatca
    38761 ttagggattg ctacttttgc tccgtctttg atatcagcta catccttcaa agtgttagaa
    38821 taaattccca gcggtgcatt gacagtattt ccgatcgata ctaaatcagt tccgtgttct
    38881 ttattgtaat tgtctaaaaa gaattgatgt tggaaagaat tcaaatcaat cgatccatct
    38941 gctaaagcca catttggttg attgtaatct gtaaatttca cgtattccaa attaatgttc
    39001 tcctttttca aacggtcttt cacgttgtcc catacatctg tatcttctcc aataatccct
    39061 agttttacga tagtttcttt tttgtcagtt gtatctgctt ttccttggct acaccctgcc
    39121 aaccaaataa ttgctacaaa tgtaatgact acaaaacgaa taattttttt cattttttct
    39181 cctccacttt caatgtgtaa tttttttaat cagttgatta ctgataaatt gacttaaaaa
    39241 aactaagatt agaatcaata atgtagccac aatcgttaca tctgtttgga atcgattata
    39301 ccctctcgat atagcaagat tccctaatcc gcctgcacct actgctccag ccatggtagt
    39361 cagtccgatc acgttgatga tagtcaacgc cgagacccga atgataccag gcaaaccttc
    39421 taacaaatac acacgtaaaa tgataccgat tggactggtt cccatcgctt ctgccgcttc
    39481 gatcacccca ggatcaactt ctagcagtgc gttctcgatt tgacgagcaa aaaacggaac
    39541 aactccaaaa atcaatggca ctaaagctgc tttcgcacca atcgtcgttc caactaagaa
    39601 acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa gggatggaac gaatgatatt
    39661 gatcagctta tcaagtattt caaatagcag acggttagct agtaatccat tagggcgtgt
    39721 cactactaac gtcacaccta agatgattcc aatcatcccg ccaatgaatg ctgtccaaaa
    39781 agacatatat agtgtttgct tcgtcgcttc gacaaattca tccggaatca aataaacatt
    39841 gggcaaatat gttttaaaga aatcagtcat tttatcttcc tccttcgatc actttgatac
    39901 gactatctaa atcgtttttt tgtaaaatat ttatttttac accagatttg atcaaataat
    39961 ccaatgcttt tttccgctgt ttggtttctc cagctaaagt aacgatcaaa ctccctaacg
    40021 gcgtttcttg taataactct acattgccat ataaaatatt cgctgttact tgatatttgc
    40081 tgtacagatg agcgattaga ggttcgttcg tctgatttcc aatataactt aattccacca
    40141 gccatttatt ggcgatctga tcggcaaaag cagaatgggc aatgatcgtt tctaatgctt
    40201 ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa ttcttcttca ggattgctga
    40261 agatctggat gctgctacct ttttcaacga ttcttccatc ttccataacc gctaccttgt
    40321 tgcagatttc tttgacaact tgcatctcat gagtgatcag gacgattgtt aaacctaact
    40381 ggcgattcaa tttttttaac aatgcaagga tctgaagggt cgttttcgga tcaagtgcac
    40441 tggttgcttc atcacataat aataccttag gttcattcgc caaagctcgt gcgattgcta
    40501 ctcgttgttt ttgtcccccc gacagttgtt taggataagc agatgctttt tcttccaaac
    40561 cgacaagttc cagtagttca ttcacttttt gacgacgttc ctgtttcgat tttccagaat
    40621 actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct ttccattaga ttgaaatgct
    40681 gaaagatcat accgatggcc tttctttctt ttcgcaattc tttgggagag agctgactca
    40741 gattcgtctg attgacaatg acttctcctg ctgttggttt ctgcagcaaa ttcatcacac
    40801 gaactaacgt acttttccct gcacctgagt aaccgacgat tccataaaca tcgccttttt
    40861 caatcgttaa atctacagaa tctactgcct gtatctcttt ttctttttgt ttgaaacata
    40921 cagaaacatc tcgcaattca atcattaaaa ttcctcctta tctaaaaaaa gcgtcctcaa
    40981 gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg tgttaccacc tcatgttcaa
    41041 gctgtgttca cacacaactc ctcttcaagt acgatcatac tctagcaaga tatcggtcgc
    41101 agccgtcatc gccttacatc tatgctcggg atgaattgct cgatggccat tttccaatag
    41161 tcgttccttg cttcttctca gctaccgaag ctctctgtca aaggttcttc tatcgtactt
    41221 accatttcaa tgcaaataat attcaaaaaa aaagtccttt tactaacata aactatgtca
    41281 gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa gtttccttcc ctcaagccca
    41341 acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg aacagttccg aatttgccta
    41401 cttatcgaca aattattctc caaggccatt ttcagctttt cttacttgac tcttctcacc
    41461 agccagagcc tctctgcaaa gtttccaaac ttactttcct tttcaacgaa atcgatattt
    41521 tgttgttggg attaatctac atgaggaaaa ctgaaaagtc aagtacgaaa ttcaaattat
    41581 tttttctata tttatttttc tgttttttta tttaacattt caataacagt tcgaagatct
    41641 tttccttccg caagaagttt caatttttcc ttttcttcga cggtataatt tcggacaaaa
    41701 ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg aagcagctgt tgctcctact
    41761 aaagaatctt ccactgcaaa ggctgaacta gcttctaatc ctgttcgttc caaagctgtc
    41821 agataaggaa tcggtgaagg cttgttctcc gacacttcgt ctccgaatgt aaacgtgtcc
    41881 aagtaatcag ctaatttaaa gtgatccaag atatctgttg ctcttttcgt aacagtggtc
    41941 gtagcaatgc ctagtttcat atgacgttct tttaagtctg ccaatatttc ttttgcatat
    42001 ggttttaagt ggatggctcc gcttgtcagc tgttgataaa aatattcttc acgcttcgct
    42061 cggacttcct gtactttttc agggctacct gctgctttca ctaaaatatc aaaactttgt
    42121 ttccagtttt ttccgcccca agcgtcaacg atttcttttg ggatatggat atcaaattgc
    42181 tgaaatcccc aaagccagcc atcacgatac gttttttctg tgtcaactaa caaaccatct
    42241 aaatcaaata ttgcaccttt catctataca ttaatccttt ccaaaacaat acacatagtt
    42301 tagcacgact ggtccaaaaa gaacagaagc aaagaaccag ccttctcgtt tataagagtt
    42361 cttggaatcg acggatatag attttcttca cagctgtcac aagcgccaga taagcgatga
    42421 ctgtagcaaa taaccataac cagtaattgc caggaagtgg catcaagcct aattcagcac
    42481 caaaacttgt aaaaggaagg acagaaccga caataattcc taatgtagtg ataacaaaca
    42541 tggcaaatgt tgcgttgctt tggataaatg gtactttcgg tgtacgcagt gcatggagca
    42601 ctaaggtttg tgtccatagt gattcgacaa accatcctgc atggaataat gcgatgaaaa
    42661 tcactcgctg tgaactatcc aatgtatgga aacttccgcc cactacagca ggacaaataa
    42721 caaaatacat caacaagtaa gtagtaatat caaatatcga acttgttgga ccaaagtaaa
    42781 ccatgaattc cccaatacta gatgcattcc atttttttgg ctcgtccaaa tattctttat
    42841 ccatccgatc ccatggcaag gaaatgcagg aaacatcata gatcagattc aaaaacaaaa
    42901 gttgtaaagg cagcattggt aaaaatggca ggaacgtact tgcaatcagc acggaaaaca
    42961 tattgccaaa attagaactt gctgtagcct tgatatattt catgatattc ccaaaagttt
    43021 ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa aggatcacgt
    43081 ctgcagattc tttcgcaata tccacagctg tatctacaga aataccgata tcagccactt
    43141 tcattgcagg agcgtcattg ataccatctc ctaaaaatcc gacagtatga ccatttttac
    43201 gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa ttttacgaat acttcatggt
    43261 cttcagctac ttgtgctaat tcatgatcac tcatttccga gattttctca ccggtaacca
    43321 attcattgat ctctagacct acttgacggc acactgaacg tgtaactaat tcgttgtccc
    43381 cagtcagaac ttttacagct acaccatgtt ctttcagagc tttcaacgct tgtttcgttg
    43441 tttcttttgg tggatcaagg aaagcaagat aaccaatcaa aaccatgtct gactcatctt
    43501 tgactgaaaa ttctccaacg acgcttggat tggtcttttg tgcgacacca atcactcgta
    43561 agccatcttc attaagtgac tgtacttttg caagtacggc ttcttttcgt tcttttgtta
    43621 atggtgtaac agtaccgcca agatcaataa atttagaaat gttcagcatt tcttcaatcg
    43681 caccttttgt gatcatttgt gttttaccag ctggatcagc aaccacaaca ctcatacgac
    43741 gacgttcaaa atcaaaagga atttcatcca ctttttgata attgatctta tctgtatcta
    43801 aagtttctgt tgcttcatca atgatagcca catctaacaa atttttcagc cctgtttgat
    43861 agtagctatt tagatacgca tggcgcaaaa cacgatcatc ttcatttcct tcacaatcca
    43921 tatgatattc aagaatgatt ttatcttgtg tcaatgtccc cgttttatcc gtacataaca
    43981 catcgattgc accaaagttc tggatggcat tcaaattttt gatcactgtt ccttttttgg
    44041 ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc atttcaggtg
    44101 ttagaccaac tgctacagat agtccaaaaa gaaaagcttc tagccaatcc ccttttgtaa
    44161 gcccattgat gacgataact gctggagcca tcaatgacat aaatttgatc agtagcatgg
    44221 atgttttatg gatgcctaat tcaaagcttg attgaattgg tttttcagat agcgtttctg
    44281 ctacatgacc aaatagtgtt tcatttccaa cagaaacaat gattccttcc gcacttccac
    44341 tgatgacatt acttcccata aagacaagat tttcgtaact tgtttcgctt tgctgatcgt
    44401 cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat gctgcttggg
    44461 aaatgaataa atcctttgcg gacaataaac gcatatccgc tggtatcata tcccctgctg
    44521 aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgtg tactttccat
    44581 tacgttttac agctgccgtt actttcacaa gtgatttcag tttttcagcc gcttgattgg
    44641 aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat aaccatagtg aagataatgg
    44701 ctacaccgaa aagatcttta tctccaggag ctgctaatac atattccgtg atgaatgaaa
    44761 taattcctaa tgcgatcaac actaatgtaa atggcgtgat gtatgctttc aatatttcta
    44821 caatgaatgg tgtttttttg ccatatgaaa tcgcattttc accgtaattt tctcttgaac
    44881 gttcgatttg ttcttctgtc aatccatttg ctgatgcatg gaatagttca aataattctt
    44941 gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt ttttccatct
    45001 caatctctcc tttgtgtcca aaagacagct gttctcgctg cttttggtct ttagctattc
    45061 atcggatcga tagagaaaaa actgaaaggg gctgagaaca gatagataat agccctggcc
    45121 tttcctctag atccttgttt ttcgaatgac tgtcatccat ttgttctcat ctcctttact
    45181 aagtaatttt aattcatgac ttccaatcat ttttcgaata gttatttttc caattagacc
    45241 acctcctttt tatttttcac cgatggggat ttgcggcaac aaaaaaacct ctcgccaaat
    45301 actcagcgaa aggtttgaaa aagcatatta aaaaaagctt ctcagatctc atcgttgagt
    45361 tttgacactg tacaacgtaa aaagcagata cctgctttta gctaccttat gaaaagcctt
    45421 atctcgacaa ttcctgttct acccattggc gtctctcgac gtttttgggc agtagcctat
    45481 gtttgtatag gagcctcacc taacaaatct tattcggttt tccttcgtta atataacaac
    45541 taaaaaagag gatgtcaaat caattttcca tgtttttcat acttttttta aaatacacac
    45601 gttatattcc tgtctcacgc ggttttattt gtctcatttt ccacctcccg ctataataga
    45661 aatatagtag gaaacgaggt gctgttatgg aaagaacact tgttattatc aagccagacg
    45721 gcgtgagacg ccatttagtc ggaagcatca tccaacgttt tgaagcaaag ggactggcca
    45781 ttgctgaaat gaagtttgaa actatgactc ctgaattagc aaaagaacat tatcagcact
    45841 tgactgagcg cagttttttt gatgaactga tcgattacat gacttccggt cctgtcgttt
    45901 atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg taagatggtt ggagcaacaa
    45961 aagctgccga tgctgttcca ggtacgatcc gaggagacta cgcgctgccg gggactgaaa
    46021 acatcattca cgcttctgat tcccgtgatg ctgcggtaaa agagatcgcc cgctttttcc
    46081 cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa gtgatacaaa ttctaaactg
    46141 ctggtctagt tgctgggttc agcaatggcc agcagttttt tctctaagga cctccgttga
    46201 ttctttccta acatatattc gagcactgtg aagaattctt ggtagtcttt cgtcttttct
    46261 tcccctaaag cagcagaaat caatctttga taataatctg ccatcaatgt ttcatagatc
    46321 actgtacttc tttctttatg tgctaagctc cgtttcatat cttcattcac actacgtgcc
    46381 tttttcaacc gaccattttc tatataaaac gaagtcaggt tcattaaaaa agtaaagaga
    46441 tactgctcat gattgttcaa ctgtgctaat cgtcgcattc tttttttgat tttcaaataa
    46501 ttgttctcaa tgaaccgatc actgaatatc caaggaaaat cagctagaat agatagttca
    46561 aattgaccat agtctggtat ctgcatcaag tattcttcaa tgattcggca ttcataacgt
    46621 aaaaattcaa attctgtctc agatacttct gttttttcga ccatgtgaag attcaaaacc
    46681 aaataataca gatcttctgt tttcttaaaa tgcctacggt tattgctgat ctctttataa
    46741 ggatcaaagc tgtcttcttg tacccgatct agcattcgtc tttttttctt taactttatt
    46801 ggttcatcca gctggtttcg catccattca tattctgcaa gtgtcatgtt cattcgctct
    46861 aaaaatgatc tcaatgttac aaatgacatt tttttcactg aatttgtctc aatcccgact
    46921 agagaagtct taaaacagat tccttccgct aattgggact gtgtcaactt cttgttgact
    46981 cgaagtttct tgatcaattc accatcatac ataatagttc tcctttttaa tttaaatttt
    47041 tccacattca ataaatagat acgttaaaaa ggaaaaatac acgaaaaaaa taaaaaaatg
    47101 tttttttcat aaaaaatacc aaccttcaag cattagaatg ttgtctaact actgaaggtt
    47161 ggtttgattg tttattgatt atttttttgc tgttcaacgt ctgttcttgt tactggatat
    47221 aagttggttg ttgttttatt accatttttt gaaaagacac ttgtcgaatg ttcgcctttt
    47281 tgcttttcga ttgcttcatt acgttccgct tcatttttat agtcatattt tttcggatca
    47341 atcgtctcaa atccgtttgg cacataaaac cgcaagagat tttcctgatt cagcttatct
    47401 gaaagactta acgcagtctt gactttttcc tgatctttgg agatttcctc atcgattcct
    47461 gctgctttcg tatcgatgat ttctcctgtc tgattattgt aagcttttcc tcctaggacg
    47521 gtgtatttgg gtgtcacaaa gttcccattt cgaaaagcaa ctacttgatc atgctgagaa
    47581 gataataagt ctgtaccaaa atgaagataa tctttatcat cgattcccaa tagatgtagt
    47641 aatgtcggca aaacatcgat ttctccaccg tattcatgat tgactgtccc tttagtataa
    47701 ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa ttcatcccat
    47761 gtatctggat ctttaccaag agcgcttgct aaatctttat tatctgtatt cgagatccca
    47821 aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa taccagattt tttcaaatac
    47881 gtaaaaaatt gttccaatga ttgatccaga taatgtgccg ttcgaacata attatctact
    47941 gtgctattgc cagtattcaa agatggaaag tcgaaattct tgtcatccgt ataataaggc
    48001 gtgtggttgg ttacggaaag aaatttcgtg taaaacggct gttgtagatg ctcaagatat
    48061 tgggcagatt ctctgaataa atctttatct aatatcccat aacctaacgt ttcgtcggat
    48121 tcatcaaaat aagatcgatc aaagaaattg tcataaccta agtttttata tacatgatct
    48181 ctattccaaa aactagctac attcccatgg aaaacagcgc ttgtatagcc ttgcttttgt
    48241 ccaaggatcg ctggtgcggc ttgaaagaca ttgtctgacc caagttctgt gaataatgag
    48301 ccttgaggta aaccaaatgt gccagtttcc aacatatttt cagcatcact tgtctttccc
    48361 tgtcctactt catgaaagaa attgtcaaag ctgatcgttg cttgattctg gaaaatacta
    48421 tttaaaaacg gtgtgacttc ttgtccgtct accttcatat taatcaaaaa ctgctggaaa
    48481 ctttctaaat ggagaacgat cacatttttc cctttagcga tcccaaatgt ttcgggattt
    48541 ggctctgcat aatgtttctt tgtataatct aaaacttcat caatcccatt actgcttgca
    48601 tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt cataaatagt atacgcatcg
    48661 attcctagat atttgacaat atatgaacgg tcaaatgttc gctgcagcaa ttgtggacgg
    48721 ttggcttcgc tcagcgctag atttcctgaa aaaataagcc ctgaaagaca ggtaacagcg
    48781 atagccatcg gtttgccgac agggttgctt ttgataggta ttttcttgac cttcaaccaa
    48841 accagtagcc caatgaacac agcgatatcc agccagtaga tgacatcatg gggcttcatc
    48901 aaagaaaagg agcttcctga caatccttgc gacactttcg aaaagcctaa tacagatttg
    48961 actgtaatga aatctgtgaa ttcccgataa aaaataatat tcaagtacaa caaaagcgta
    49021 tttaaaatat caatgatcaa aagaatagct aacgctggct ttggcttttt aatatacaaa
    49081 gaaaggccaa aaaacaataa ggtagtcgca attgggttga tccacaagat aaagtactgg
    49141 atcgtacctt ccacacctaa tgaaaaatct gcatagtagg caatgatcgt cttcagccag
    49201 aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt tttaagttgt
    49261 gtcacgtata tcacttctct ctattcacat tttttcttat tttgctcctg aaaaatgaaa
    49321 atttcctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat tattccgtcc
    49381 acttttcaca gcataaaacc gctgcgatgt aagcgattaa actaatgaag agataaaagt
    49441 cttgtggtga ccgcttcact ccacggtatt caaaagccag tttctcggta ttcgtacaaa
    49501 aaaagaggct gtgacaaaat tcttgtcgca gctccttatt tcttgatgca ggacagcttt
    49561 ttcacaactt cttccttaaa cggtgttctc ctgccaactc ctcggccaca agtcacaata
    49621 tttataaaac atgagaagct gttttttgaa attgttcctt gtggctcaga gttaaacggc
    49681 aggctcaaga cctcttgata cacggtgttc aatcagctga ccctcggtat tagattatgt
    49741 caggacgcta attttacaac ctcttggtaa acggtgttca aaagcttgca tctgcggtaa
    49801 taagcccaac caaacgaaaa atataaggag ctattttttg aaattgctcc ttgtggctca
    49861 gagttaaacg gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccttcggt
    49921 attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc
    49981 aggtcaaaac gctgatttca caacctctgt ttttcattgt ccataggatt gatattgtcc
    50041 ttgtgatgac tcattcgtaa taattgaggc ttgattttct tcacttcccg ttatcgtttg
    50101 tccactgcca gaatctgaag gacttgttcc tgtcgactcg tttttttgag aagagacagt
    50161 tgttttcttg acttgttcat ttgtcttttt ccattccgct aaacgatcgg cgaccacttt
    50221 tttgtctact ttttcagaat aagcggtgat cacttttcca tttttcaaac gggctacttt
    50281 gtcgccatca tgagtcaaaa gcaaaacggt caaatgcttg tctttaaatt gatattgtct
    50341 gagagaaatc gcactaagta cgtcatcgat attaataaat tgattttctt cttttccttt
    50401 tggcaaataa atgtccaatt cgcctttttc gtaattggca gttattttat actccgccca
    50461 ccaataagtt gagaattctt ttttgaaatt atctactgat tctttgcttg ctacgatttt
    50521 ttctgtttct ttctgagcaa gatctgctgt catttttcct gaaaatgctg cggataaaat
    50581 aagcagtaag aaaaaaccga ataaccaaaa gagaagaaac ggtctttttt tcctcatgct
    50641 ttcctccact cctatttaat tttgactatt ctattatata caaaaaatga aatagaaaga
    50701 caaaaaagtt gaaaaagatc gtaaataatt gaacttcttt ttcacaaaat ggtaagaatg
    50761 gagaatttca cgcatttgct ctcaactatt ctttcttaag cctcaaatcc gtttttatct
    50821 gatacgtctt tgaaccaatg acccgatttt ttgattgttc tttttccatc ttgtgctaag
    50881 tctactgaaa taaagccata tcgattctta tatgcattgg tccacgacca gttatccata
    50941 catgtccaca tatgatagcc taaacaattg gcaccttctt gaatggcttg atggacatat
    51001 ttcagatgag attggacaaa ttcgatccga taatcatcgt ggatcatgcc atcttctttg
    51061 ataaatcgtg cttcgttttc tacacccata ccgttttcag aaataaagca gtcgatattt
    51121 ccataatgat ctcgtacatt gatcaaaata tcatagattc cttgttcata gatctcccat
    51181 ccgcgataag gattcatttt acgaccaggc atttcataat tgtcgaaata atcctcaggc
    51241 attggaccgt ttttagtatc gattggtgtt tcttttgctt ttacccggcg tggctggtaa
    51301 taattgatac ctaaaagatc tactgtattt tctttgataa ccgtcaaatc ctctgcatgg
    51361 atcacaggca tgagatcaag ttcttttact atctcaatca attcacttgg aaactcgcct
    51421 tttacagctg gatcaaggaa tgaacgattg aagaacgcat ctgctatttg cgctgcttta
    51481 acatcttcag ggtttgattc gtctcttgga tagcttggtg tcagattcaa gatgatacca
    51541 ataacaccac cttgctccat ttcatggtaa gatttgatgg ctaacgctga agccaacgct
    51601 tcatgatagc caacttggat cgcttttttc atatctgact cttctggata atgccatcga
    51661 tataaataac ctgcttcgac tggaacgatt ggttcattat gagtaaacca tttttttact
    51721 ttcgaaccaa acaattcaaa acaacttttg gcatattttt cataggcttc aaccgtttct
    51781 cggttcaacc atccgccttt ttcttgcaga tacatcggca tatcgaaatg atacagattc
    51841 acaaagggtt cgataccgtt ttcaatcaac tcatcaaaat aacgatgata gaaacgaacg
    51901 gcttcttcgt tgattgttcc agattcatca ggtatcaaac ggctccattg gatagatgtt
    51961 cgaaatgaat tatgcccagt ctgtttcatc aactgtacat cctcttgata cttcgtataa
    52021 acttgcgagg ttttttcagg tcctacttga tcaaagaact tttgcggttc ttttttgtac
    52081 caataatcaa aaatgttctc tccttttcca tcgcctgaaa tccttccttc tgtttgtggg
    52141 ccacttgctg cagaacccca ccaaaaattt gctggaaatt gatatttttt catcaaagca
    52201 cctctttttc ttttttagtt cgttttagtt gattccatgt atgatatttt ccatcacaat
    52261 cactttttca ttttcttcct cttctttttt gattcgtttt aacagctgtt ctgctgctcg
    52321 ttccccgatt ttatctgttg gtggcgtgat cgtgattttc ggaaggatac tcagatcgtt
    52381 tttatctaaa taatcatagt gactgaacgc aatatccttc ccgattctga caccttcttg
    52441 attgaaacat tcgatggcac caatcgacat attgtaattg ctgacaaaaa tagcagttgc
    52501 ccctttttct aataatgctt ttgccccgta atagccgctt tttctagaat agtctcctgt
    52561 aaagacctct aattggatcg atggatcatt ttggaccgct tctttggctc ctgctaaacg
    52621 ttctcttgct acgtagtctg tttgagtggc agcaataaat ccgatttttt tatgtccttg
    52681 ttcaccatga tgagcgatca cttttgctac actttctcga tcattcacta atattgtatc
    52741 aatacctgct ggtccttctg gacaattcaa tgcgataact ggaatactca tttttgtgag
    52801 catttcaaat ccagaccact cttcttcgga taagaagaca atcagcccat cgatcacatg
    52861 cttcatcagg tattcaattt tttgttcaat tttttctggc tgatcggcaa acccgcttaa
    52921 taataagcta tacccatttt cttcagctac tctttcgatc cctgagacaa cttccgaccc
    52981 aaaacggctg gaaatcccgt ttatcaaaag tccaatagag aagcttcgat tattcttcaa
    53041 ccctttagca atgatgtttt ctttatagtc caacgctgaa atcgcttctg ctattttttt
    53101 catgttcttt tccttcagtt gctgaccatt caagtaacgg gaaactgttc ccactgaaac
    53161 accagccatc ttagccacat cacggatcga tgccatacat tctcactcct gccagttaca
    53221 tttgatttct ctttcttgtt gttcgattat gatgaccgca ttctttgcat agtgttgctt
    53281 gtcatcaata tccatataac taaagtcttc agtagagcaa cttcctctca ctattaatta
    53341 tacttaaaca agtatttgaa ccggttcatt tttaatatag cattttttta aaagattgca
    53401 acactttttt aaaacgcttt ctgaaagtgg cggatttttt gcctttttaa aattttattc
    53461 taaaatttac tctatttgtg ggtgttttgt gcgtttttct cttccatccg tttgaagttt
    53521 tacattccaa tggtataatt tctttatcct atcaaatggg ggaagtattt tgaagaaatt
    53581 aatcgtacta tttattttgt ctgcgggact tttgacattg actggctgtg atttgtttgg
    53641 cggctctgac caatcctcgg acgaaacaac cgattcttct gtcttaaaag cggaaaaagc
    53701 agataccacc aacagtgtat atacgctaaa agtacgtaaa gatatggatt tatccgcggg
    53761 acagttgaca tttacagata aaaaaatgga atggacacgc acgtatcaga cacaaggaac
    53821 tgattcttct gaatcatctg aagaaaaaac agtgctgaca gatatcaaga tcactaccaa
    53881 agatgacgaa tatattattt caggaaaaga aaataaaaaa gaaacaacag tcatatttac
    53941 aaaaatcggt aattaccgtt tgaaggatga ggatggcaac atctacagtc tatagtgccg
    54001 tctgttggat cttcttttgt ttctcccctt cttttctgct atagtaaaga agcaagatga
    54061 ttgtaaagga gacgaaatgg atgatcgata gtcctgatta ttgtacatgt aataacaaca
    54121 aaaatatcca tacagaattt aatgaccaag gaagcttgag catatgcgac aactgtcaca
    54181 aactagtcaa agattcattt aaaacatttg aatcttatcc tgttcaaaat tatgtaaaaa
    54241 gtagaagaag ataaacaaat ccaagcaaaa ctcactatgg ctgattttta tgaacttcaa
    54301 actcagcttc ttcctttgat caagcttcat cagtaaaaca aacagtggat catttcagtt
    54361 tacaaatcaa agagtaaaga tttatttatt tcaaatattt gaatataaaa taaccaaacg
    54421 atagaaaggc ttattctaca tcacgtagta
//
LOCUS       DAYYBH010000012        53882 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000012 DAYYBH010000000
VERSION     DAYYBH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.012"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(440..943)
                     /locus_tag="ACHAUG_000826"
     CDS             complement(440..943)
                     /locus_tag="ACHAUG_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8479938.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1302..2327
                     /locus_tag="ACHAUG_000827"
     CDS             1302..2327
                     /locus_tag="ACHAUG_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8479939.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2650..3528
                     /locus_tag="ACHAUG_000828"
     CDS             2650..3528
                     /locus_tag="ACHAUG_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM8479940.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4004..4120
                     /locus_tag="ACHAUG_000829"
     CDS             4004..4120
                     /locus_tag="ACHAUG_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8479941.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5048..5386
                     /locus_tag="ACHAUG_000830"
     CDS             5048..5386
                     /locus_tag="ACHAUG_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8479942.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5480..5710
                     /locus_tag="ACHAUG_000831"
     CDS             5480..5710
                     /locus_tag="ACHAUG_000831"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8479943.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6110..6481
                     /locus_tag="ACHAUG_000832"
     CDS             6110..6481
                     /locus_tag="ACHAUG_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8479944.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6755..7447)
                     /locus_tag="ACHAUG_000833"
     CDS             complement(6755..7447)
                     /locus_tag="ACHAUG_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8479945.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7450..8235)
                     /locus_tag="ACHAUG_000834"
     CDS             complement(7450..8235)
                     /locus_tag="ACHAUG_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8479946.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8472..9872
                     /gene="lacG"
                     /locus_tag="ACHAUG_000835"
     CDS             8472..9872
                     /gene="lacG"
                     /locus_tag="ACHAUG_000835"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8479947.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9897..10214
                     /locus_tag="ACHAUG_000836"
     CDS             9897..10214
                     /locus_tag="ACHAUG_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8479948.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10227..11900
                     /locus_tag="ACHAUG_000837"
     CDS             10227..11900
                     /locus_tag="ACHAUG_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8479949.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11914..13545
                     /locus_tag="ACHAUG_000838"
     CDS             11914..13545
                     /locus_tag="ACHAUG_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8479950.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14036..15601)
                     /gene="guaA"
                     /locus_tag="ACHAUG_000839"
     CDS             complement(14036..15601)
                     /gene="guaA"
                     /locus_tag="ACHAUG_000839"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8479951.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15660..16583)
                     /gene="coaA"
                     /locus_tag="ACHAUG_000840"
     CDS             complement(15660..16583)
                     /gene="coaA"
                     /locus_tag="ACHAUG_000840"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8479952.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16779..17384
                     /locus_tag="ACHAUG_000841"
     CDS             16779..17384
                     /locus_tag="ACHAUG_000841"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8479953.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17692..18708
                     /locus_tag="ACHAUG_000842"
     CDS             17692..18708
                     /locus_tag="ACHAUG_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8479954.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18849..20150
                     /locus_tag="ACHAUG_000843"
     CDS             18849..20150
                     /locus_tag="ACHAUG_000843"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8479955.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20196..20858
                     /gene="deoC"
                     /locus_tag="ACHAUG_000844"
     CDS             20196..20858
                     /gene="deoC"
                     /locus_tag="ACHAUG_000844"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8479956.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20879..21277
                     /locus_tag="ACHAUG_000845"
     CDS             20879..21277
                     /locus_tag="ACHAUG_000845"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8479957.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21343..22425
                     /locus_tag="ACHAUG_000846"
     CDS             21343..22425
                     /locus_tag="ACHAUG_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8479958.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22637..24199
                     /locus_tag="ACHAUG_000847"
     CDS             22637..24199
                     /locus_tag="ACHAUG_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8479959.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24192..25325
                     /locus_tag="ACHAUG_000848"
     CDS             24192..25325
                     /locus_tag="ACHAUG_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8479960.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25325..26281
                     /locus_tag="ACHAUG_000849"
     CDS             25325..26281
                     /locus_tag="ACHAUG_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8479961.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26380..27546
                     /gene="deoB"
                     /locus_tag="ACHAUG_000850"
     CDS             26380..27546
                     /gene="deoB"
                     /locus_tag="ACHAUG_000850"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8479962.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27570..28388
                     /locus_tag="ACHAUG_000851"
     CDS             27570..28388
                     /locus_tag="ACHAUG_000851"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8479963.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28405..29112
                     /gene="deoD"
                     /locus_tag="ACHAUG_000852"
     CDS             28405..29112
                     /gene="deoD"
                     /locus_tag="ACHAUG_000852"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8479964.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29410..30096)
                     /gene="gpmA"
                     /locus_tag="ACHAUG_000853"
     CDS             complement(29410..30096)
                     /gene="gpmA"
                     /locus_tag="ACHAUG_000853"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8479965.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30268..30849)
                     /locus_tag="ACHAUG_000854"
     CDS             complement(30268..30849)
                     /locus_tag="ACHAUG_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8479966.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(30982..31665)
                     /gene="rpiA"
                     /locus_tag="ACHAUG_000855"
     CDS             complement(30982..31665)
                     /gene="rpiA"
                     /locus_tag="ACHAUG_000855"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8479967.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            31984..32397
                     /gene="rpsL"
                     /locus_tag="ACHAUG_000856"
     CDS             31984..32397
                     /gene="rpsL"
                     /locus_tag="ACHAUG_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8479968.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32481..32951
                     /gene="rpsG"
                     /locus_tag="ACHAUG_000857"
     CDS             32481..32951
                     /gene="rpsG"
                     /locus_tag="ACHAUG_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8479969.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33045..35132
                     /gene="fusA"
                     /locus_tag="ACHAUG_000858"
     CDS             33045..35132
                     /gene="fusA"
                     /locus_tag="ACHAUG_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8479970.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35304..36491
                     /gene="tuf"
                     /locus_tag="ACHAUG_000859"
     CDS             35304..36491
                     /gene="tuf"
                     /locus_tag="ACHAUG_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8479971.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36898..37206
                     /gene="rpsJ"
                     /locus_tag="ACHAUG_000860"
     CDS             36898..37206
                     /gene="rpsJ"
                     /locus_tag="ACHAUG_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8479972.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37237..37866
                     /gene="rplC"
                     /locus_tag="ACHAUG_000861"
     CDS             37237..37866
                     /gene="rplC"
                     /locus_tag="ACHAUG_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8479973.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37894..38517
                     /gene="rplD"
                     /locus_tag="ACHAUG_000862"
     CDS             37894..38517
                     /gene="rplD"
                     /locus_tag="ACHAUG_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8479974.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38517..38807
                     /gene="rplW"
                     /locus_tag="ACHAUG_000863"
     CDS             38517..38807
                     /gene="rplW"
                     /locus_tag="ACHAUG_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8479975.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38846..39679
                     /gene="rplB"
                     /locus_tag="ACHAUG_000864"
     CDS             38846..39679
                     /gene="rplB"
                     /locus_tag="ACHAUG_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8479976.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39722..40000
                     /gene="rpsS"
                     /locus_tag="ACHAUG_000865"
     CDS             39722..40000
                     /gene="rpsS"
                     /locus_tag="ACHAUG_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8479977.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40023..40370
                     /gene="rplV"
                     /locus_tag="ACHAUG_000866"
     CDS             40023..40370
                     /gene="rplV"
                     /locus_tag="ACHAUG_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8479978.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40384..41040
                     /gene="rpsC"
                     /locus_tag="ACHAUG_000867"
     CDS             40384..41040
                     /gene="rpsC"
                     /locus_tag="ACHAUG_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8479979.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41043..41477
                     /gene="rplP"
                     /locus_tag="ACHAUG_000868"
     CDS             41043..41477
                     /gene="rplP"
                     /locus_tag="ACHAUG_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8479980.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41467..41655
                     /gene="rpmC"
                     /locus_tag="ACHAUG_000869"
     CDS             41467..41655
                     /gene="rpmC"
                     /locus_tag="ACHAUG_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8479981.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41680..41946
                     /gene="rpsQ"
                     /locus_tag="ACHAUG_000870"
     CDS             41680..41946
                     /gene="rpsQ"
                     /locus_tag="ACHAUG_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8479982.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42004..42372
                     /gene="rplN"
                     /locus_tag="ACHAUG_000871"
     CDS             42004..42372
                     /gene="rplN"
                     /locus_tag="ACHAUG_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8479983.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42409..42717
                     /gene="rplX"
                     /locus_tag="ACHAUG_000872"
     CDS             42409..42717
                     /gene="rplX"
                     /locus_tag="ACHAUG_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8479984.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42745..43284
                     /gene="rplE"
                     /locus_tag="ACHAUG_000873"
     CDS             42745..43284
                     /gene="rplE"
                     /locus_tag="ACHAUG_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8479985.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43303..43488
                     /locus_tag="ACHAUG_000874"
     CDS             43303..43488
                     /locus_tag="ACHAUG_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8479986.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43524..43922
                     /gene="rpsH"
                     /locus_tag="ACHAUG_000875"
     CDS             43524..43922
                     /gene="rpsH"
                     /locus_tag="ACHAUG_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8479987.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43954..44490
                     /gene="rplF"
                     /locus_tag="ACHAUG_000876"
     CDS             43954..44490
                     /gene="rplF"
                     /locus_tag="ACHAUG_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8479988.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44651..45007
                     /gene="rplR"
                     /locus_tag="ACHAUG_000877"
     CDS             44651..45007
                     /gene="rplR"
                     /locus_tag="ACHAUG_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8479989.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45028..45528
                     /gene="rpsE"
                     /locus_tag="ACHAUG_000878"
     CDS             45028..45528
                     /gene="rpsE"
                     /locus_tag="ACHAUG_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8479990.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45543..45722
                     /gene="rpmD"
                     /locus_tag="ACHAUG_000879"
     CDS             45543..45722
                     /gene="rpmD"
                     /locus_tag="ACHAUG_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8479991.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45766..46206
                     /gene="rplO"
                     /locus_tag="ACHAUG_000880"
     CDS             45766..46206
                     /gene="rplO"
                     /locus_tag="ACHAUG_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8479992.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46206..47501
                     /gene="secY"
                     /locus_tag="ACHAUG_000881"
     CDS             46206..47501
                     /gene="secY"
                     /locus_tag="ACHAUG_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8479993.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47563..48210
                     /locus_tag="ACHAUG_000882"
     CDS             47563..48210
                     /locus_tag="ACHAUG_000882"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8479994.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48401..48619
                     /gene="infA"
                     /locus_tag="ACHAUG_000883"
     CDS             48401..48619
                     /gene="infA"
                     /locus_tag="ACHAUG_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8479995.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48652..48768
                     /gene="rpmJ"
                     /locus_tag="ACHAUG_000884"
     CDS             48652..48768
                     /gene="rpmJ"
                     /locus_tag="ACHAUG_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8479996.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48786..49151
                     /gene="rpsM"
                     /locus_tag="ACHAUG_000885"
     CDS             48786..49151
                     /gene="rpsM"
                     /locus_tag="ACHAUG_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8479997.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49179..49568
                     /gene="rpsK"
                     /locus_tag="ACHAUG_000886"
     CDS             49179..49568
                     /gene="rpsK"
                     /locus_tag="ACHAUG_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8479998.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49648..50586
                     /locus_tag="ACHAUG_000887"
     CDS             49648..50586
                     /locus_tag="ACHAUG_000887"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8479999.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50614..50994
                     /gene="rplQ"
                     /locus_tag="ACHAUG_000888"
     CDS             50614..50994
                     /gene="rplQ"
                     /locus_tag="ACHAUG_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8480000.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51266..52105
                     /locus_tag="ACHAUG_000889"
     CDS             51266..52105
                     /locus_tag="ACHAUG_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8480001.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52081..52950
                     /locus_tag="ACHAUG_000890"
     CDS             52081..52950
                     /locus_tag="ACHAUG_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8480002.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52940..53737
                     /locus_tag="ACHAUG_000891"
     CDS             52940..53737
                     /locus_tag="ACHAUG_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8480003.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        17175 a         9654 c        11599 g        15454 t
ORIGIN      
        1 acttctaaaa actactgttt ttttcctttg aaatgccttc aaaaagtagg atcaggcaga
       61 atatactggc agaatcaagg agaagggagc ctaaataagg agaaaaacca tagaaaatat
      121 aaagtaatta cttataaaac aagcttttta tagaaaaata atatacctga gtagggtata
      181 aattaagacg gaattccttt ctagattgag atattgacta ggaagaatgt taagatttta
      241 agcctttaag ctaaaaaaaa accctttttg ttaaataagg ggaatatagc ctgtcagttt
      301 caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa tagatttttt
      361 taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg tctattacgg
      421 tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat aaaaaagcta
      481 cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct aacttatttg
      541 tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct cctgttgtat
      601 tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga atcaattctg
      661 ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt ctcttgaaat
      721 tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg gtctcacgta
      781 ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca ttgctgtaag
      841 aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac cataatagaa
      901 agaataaata aatccctaat aaaaattttg atacataccg catagacatc tcctccattt
      961 aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt ttaaggacca
     1021 acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt ttctttaaca
     1081 aagctaagat agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa aacgactcgg
     1141 aatgttcgtt ttttgttcta atttctccaa gttgtttact ttttgatact aagtttatga
     1201 aaaatcaaga aatagttgtt atactaggta atgctgacaa acgggattgg catttcatct
     1261 tggttcagct ttttttccaa ttactaggaa ggagggatag attgttcaag acttattttc
     1321 gtttcaataa aatacaagat aatccttttc gtatcatgaa tctgatgcta cttatcttta
     1381 ttgtattatg gttaggttac ataaatcatg attttttgat ttcaagtttt tcatcattag
     1441 ggatctttac ttttttctat tatcaaaaca taccgatgaa atatttgctg aaacgtatgt
     1501 ttctgatcgg ttgcggactg ttggttgctt tcgttttggg gatcttatcg acttatgttt
     1561 tatggcttga accatttgcc gtagctttgg ttgctttttc cagcagattc gtgttgcgat
     1621 tattccatat ttctaaacca ggtggcttgt tttttgcgat gctttctgcc atgggaacga
     1681 gcatgcagct gccaatcgct caattaccga tcgtctcact ttattttttt atgggtgtcg
     1741 tttttgccct gattgcggca gtgattacaa aattactgga ttcacggcct gagcagacaa
     1801 ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc tttagtgatc attgattctg
     1861 ttttctacag cgctgcctta tttctttccg tttatgtcag tcacggcttg aatctgcata
     1921 atccttattg gttgaccttg tcatgtgcat cgattttgct agcagaaaac ttggatgcga
     1981 tgaagcatcg acaagtccaa tatctgattg gatcgatggg tgggttatgt gtgtcggcat
     2041 tcttgtcatt tgtgccgttt acgcagttgc agaccatttt tctgatcact tttctatatg
     2101 gaatcgcaca atttctagtt gctagaaatt atgcagttgc taatattttt cttaatccga
     2161 tggcgttgat gctatctaca cttattcgtg gagcatactt gatttctttg atcgaatatc
     2221 gttttttagg gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg gtaatgacgg
     2281 ttggtttgca acattattta acggttgttc gtcaaaaatt agaatagaag gacaaatata
     2341 tgtgccaaaa gaggttatga caagattttt gtcacaacct ctaattcaag gtgttcaaaa
     2401 gccagattct cagcattaag tcaaattcac acaaaaatgt ggaaaggaac tttcaaaaaa
     2461 attttatatt tattctatca aaaaattttg tttatgatgg taactgaata aactttcctg
     2521 agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag aaagtttaag
     2581 ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg ataatcagtc
     2641 atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa aataagcaat
     2701 gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga gcaaatgatt
     2761 gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga actaatggat
     2821 cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga ggaacctttt
     2881 gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca
     2941 ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag atttattcat
     3001 gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat ttggcattac
     3061 actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata ttttccgaaa
     3121 gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat tccttccgat
     3181 gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg tttttttgaa
     3241 ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt actatcaccc
     3301 aatcttacta aatggttgaa aaaagagtca aaaatctgtc tttttgcagc cgattatcga
     3361 ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg acgagtagat
     3421 gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa agagcaattg
     3481 cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt ttagtacttc
     3541 gaaatagttt aaaagagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac
     3601 acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     3661 ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag
     3721 cctctttttt agtgttctat cgaacacaca gtacgttcaa taggtaaact agctataaca
     3781 atagatatta aagttaacgg acaagagggc cttttttagg tagctaaaac gtaataatga
     3841 aaatttattt ccagagatag gagtcaatca gctagtctga tcgactgttt taacaagttg
     3901 actttcattt taacttttga taccatttaa aacacaaatc gtaattgttt cgatttgtgt
     3961 tttttagcat gactacaatc aaagaagaaa gtgggtcgtc catatggaaa aaacagatat
     4021 ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa
     4081 gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa
     4141 aaaaatagcc gaaaaacgtt tttcggctat tttcaataat cggtttttgt gtaaggtttc
     4201 cgaaagaact gctgttgatt ttccattttt aacatatgat aaattggtag aatctttctt
     4261 ttcaaattga taccattccc taattccagt tatatagtta actacagaac cacaaagcac
     4321 gtacgtttat aaaagtatta tctgtagtta tttttcaatt ccctttcaac ctcttatttt
     4381 gaccagtagt attgatcact ttacctttta acacctttgt tcgtttttga aacattttta
     4441 gtcaagaatg gaaacgtaac tacataatca aaggttttgc gaaatatttc atgaaatatt
     4501 tcatgtagaa gcgagcaata cggttgcaaa taaccattat cgtttaataa gcagttacga
     4561 aagctcccaa tatctctaac gttcagctca ctccatttct tttcttcaaa aaaatcagtg
     4621 attcgtatct ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata
     4681 tgcaaaaaaa taaaaaagca gttactattg ctagaataac atcaaggaat aataagtttt
     4741 ggttttcaga taatagtcga taagttgtgt attggaattt tagatacagt aataaagtat
     4801 ttttcatgaa attttgtgag aatcactatt acaagtatct ttaataaaat taatggttaa
     4861 ggaggaactt aaagtagaat gtaaatgaat agttgtagtc agtcgattgt gatactagac
     4921 tcaaagttgc aggaataagt ttttcaattt gtgattggaa gataaaaaat gaaaaagaag
     4981 ggcttttttt tatactttgt tgattgtagt gtttattttc cctctgttta gccaagtaac
     5041 attttctatg actcaaccag tttcaagtga ggaacatgat aacctttttc gtgctacaga
     5101 agagtttaag aaatatcagg aaaatattgt tccggagact ccattagcta gtgagccaat
     5161 tactgatatt gatggaaacc aaataggatg gtatatacag tacgaagtta tggtggtaaa
     5221 taatgactca atagtaaaaa atacagtagg cttagagcta acaccgattg aaaaggtgag
     5281 ttcattgtta acgtatattt atactgatga aagtgacgag actgagatca ttttaacgga
     5341 ttactcaaaa attgctactg ataaaaagta tatattgtgg atgtagacac tagtgaaata
     5401 ctttatgata tagaaattga tgaaaattta ctagatgtag cacaagaaat cgatcatcaa
     5461 aagcaggaag ctattcgata tgctggtaga aaagggaaaa gaaaatattt attacgtgaa
     5521 tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa ttcaagagtc gctactcaat
     5581 taactcgact ccgacattta cagtttacag agaaggatcg atagaaaaaa ctgttttttg
     5641 gactaaagaa agtggaatgt ctttagctga agtagaagag tttttggatt atgttagtat
     5701 gcaacagtaa gggagttctt attatgaaaa attttgacac atattatgtt attgtttttt
     5761 cgatatcttt gctgttaata ttactaactt taatgcagta taaagagtat ccagagtatt
     5821 ttattttaac ttttgtatat ttagtaagta gctacacact tctatataaa ttcagaagtt
     5881 cttctagagt ttctaaagtt tgtttcttaa ttagcttatt gattctataa tatttttgat
     5941 ttatatagga aattaaaaat attatagaat caacactcag agaacgggtt ggtttttaat
     6001 agatttaata agtactgata atgaaatttc tcctggttgg gaaaacgaag ttggttaccc
     6061 tgtagaagaa ttttttcaag attactttgc ggagtatgcc gataggataa tgagagatta
     6121 cgtaacagat aaaactcaag aagtcatcaa tcaatcccag tcattaaatg aattatctga
     6181 taaggcgaaa gaagaagcag ttgccattat ttcttcaggg attagtgact atattgatat
     6241 gagtggtgaa atcaattatg gcgataaaga caaaattgta cgcatgataa aagcttttat
     6301 caaaactttt gatgtgaagg cagatcttaa gtttaatgat acttacctag taggaaaatt
     6361 aattaatatc cttgggacag agaaagatac ggaaacgttt atttcctctt tgtctatgtt
     6421 gtttacagat aagcattttt caactcagct tggtggagag gaactagtgg aaatctttta
     6481 accattacgc ccagcattaa taaaagtata tcaaaaaagt actcctgatg atttttaaga
     6541 ttggtttgag aaataatgtg aaaacactat ttttaatgtt aattatagat agagatgaac
     6601 gcgtcatatg gttaaaagaa tgaaaaagtc aaagatacac tgctatattt cactaaggat
     6661 atagcagtgt atctttcatg tcgtttcact ggcagttttt tataaaagga ggtgtgcatt
     6721 tcagttgctt ttctttggta gttagttgaa tcgtttaaga attaacggat gtggcagaca
     6781 ggtgctctgt ccaaaaacca gtaaaatctt tgatggttcc atctttattt agctgtgcct
     6841 gttcaaatac tgttttgttt ttaccgaatg gatcaattgc aataattgtt ccgatccccg
     6901 cacgtttggc agcaagtaat cctgagtaag catcttcgat aacaagacaa tcagccggag
     6961 ctaagctcag tttgtctgcc gctttaagaa aaatatccgg ttgcggtttc ccagggaaag
     7021 agccgtcgtc atagacaatc ttgtcccagt caaaccagcg ggctaaatcg aaaatatcaa
     7081 aataaaaagc aacattctct ttgacagtag ccgtggcaat cgttaatgga atctgttggt
     7141 cagtcagctg gtcaagagtc gcttcaagcc cttttgtcaa ctgaagctcg ttttcatttt
     7201 gcaagcaaag catacgataa tagctttctt tttcttctga caatacggcg atctcagagg
     7261 cggtcaaatg ctctgaaatg aaatgtttca aaatctccgt attcgtccga ccatgaatat
     7321 tttttaaaat atcctcgtct gataaaccat cttttgtatg atcatgtatc atatgcagcc
     7381 aagcagcttc atgcagatga gagtccaaaa acattgttcc gttgaaatca aatataatcc
     7441 ctttcatagt tacgctccta tagtggtatt ttcggttgaa tcagcaattt gcagatcgac
     7501 atattgtgca tagtgctctt gaacctcccg cgagatctgg cgatccgtaa tgagcagatc
     7561 gaaatcatgc aaattataaa acacataaaa atcatctcga ttgaattttt tattatcaac
     7621 gagtagatat ttcttacggg aattatttag agcgatctgc tgtgcttctc cttcttcggt
     7681 actgtaagta gagatcgctt cgttgtgaac gccatttgaa ctgataaatg cttttgtaaa
     7741 ctttagtctt cttaaattgt cgttggtgat cggtccgaca aatgcgccgg tatttttccg
     7801 gaaatcaccg ccaatcagta gaatttctgg tccatctgct tgagcaagaa tatcaaaaac
     7861 tggataatta tttgtaatga tccgaatttt cttgttcagt aaatgcgtcg ccaatagttc
     7921 gattgttgta cctggtccta agaaaatcgt ttctccctcg tgaatcaagg aggctgctgt
     7981 ggcagcaatt tctttttttt cttgaatctg cagagtggct ttttcagtgt gagaaagttc
     8041 ttgatctaaa gtgtaggaaa gactttgagc ccctccatgg acacgcagca gcttgcccga
     8101 tttttctaat tcatctaaat ctctgcgaac ggtcatatca gaaacatcta attgattgat
     8161 gatttcatta acggtaagga ttcctttttt attgaccatt tctacgatag taagcaatct
     8221 ttcttttttc aacattgtct tcacctctaa tttaaggata atctatcata taaacggaca
     8281 acagtcaatc taattcgaac aaaagtaaac atttagggtg gtttttatgt ttgttacaat
     8341 agattttatt gctgttttct ggtttttatg attatttgtg tttgtttttg ttgacaaatg
     8401 ttttggatgc gtttacaatg caatcagtta gaaagagcga aaactgatta tagatttgag
     8461 gagagatgta catgcttaaa ttaccagaag attttatttt tggcggagcc acagcagcct
     8521 atcaggttga aggagccact aaggaaggtg gcaaaggcgc tgttgcttgg gatgactttt
     8581 tagaggaaca agggcgattc agtcccgatc cggcaagtga tttttatcat caatatgcaa
     8641 aggacattga attgtgtgaa cgttttggtg taaatggtct aagattatcc attgcttgga
     8701 gccgcatttt tcctgatgga gctgggaaac caaatccaga aggaatcgct ttttatcatc
     8761 gtgtgttcga agaatgtaag aaaagaaacg taacgccgtt tgtgacgttg catcattttg
     8821 atacccctaa acgtttgttt gaccatggag attttttgaa tcgagaaaca atagaagcat
     8881 ttgtttccta tgccattttt tgttttcatg aattcaaaga agtcaaggtg tggagtacct
     8941 ttaatgaaat ctatccagtt gcaaccaatc aatatttgtt aggtgttttc ccaccaggaa
     9001 tcaaatatga ttttacaaag atcgtcgcct gtcttcataa tatgatggtt gctcatgccc
     9061 gtgtagtgaa ctatttcaaa gaaaacgaac ttccgggtga aattggtgta gttcactcat
     9121 tagagacgaa atatgcggca acagacgcgc cggaagataa gcatgctgcc tttttagacg
     9181 atgcattgtc gatccgtttc ttattagatg ctacctattt aggctattat tcaactgaaa
     9241 ccttgactgc tcttgatgag atatgtgaag caaatcaggc cagctatcat tttcctgaag
     9301 aagatttcgt tgaattgaaa aaagcatcca cgcgtaatga ctacttaggg atcaatcact
     9361 accaatgcca ttttgtcaaa gcttacgatg gagaaaatgc aatccatcat aacggtacgg
     9421 gtgaaaaagg gacctctgtt tataaagtga agggaatcgg agaacgtatt tataaagaag
     9481 gaattcctcg aactgattgg gactggctga tttatccaga aggtctatat gacttgttgc
     9541 tgcgaattaa aagcgattac ccacattaca acaaaatcta tatcactgag aacgggatgg
     9601 ggtataaaga tcagtttgaa gatggcatta ttatggatca gccgagaatc gactatttga
     9661 gagtctattt ggaatcctta agcaaggcga taacagccgg ggtaaatgtg aaggggtatt
     9721 ttttgtggtc gcttatggat cttttctcat ggacaaatgg gtataacaaa aggtatggct
     9781 tgttttatgt tgattttgaa acccagaagc gttacccaaa agaatcagct tactggtaca
     9841 agttggtcag tgaaaccaaa acgattattt aataagtgat aatggaggaa gaaaagatga
     9901 ataaagagga attacaaatg cttggttttg agatcgttgc atattcaggc gacgctcgca
     9961 gtacattatt gaaattattg aaagaagtaa gacaaggaaa ctttgaacat gttgattcgg
    10021 cgttgaaaga agccgacgaa aatttaaccc ttgcacataa ttcgcaaaca aaaattttag
    10081 cggaagaagc atctgggaaa gagatggaga tgggattcat ttttatccat gggcaggatc
    10141 atttaatgac cactttattg ctgagagatc tgattcaaga ctttattgta ctgtacagac
    10201 agaatcaagg ataggagaag aacgctatgg atggaatcat taaacaaatc gaaaaaggga
    10261 aaccattttt tgaaaaaatc tcacgtaata gttatttagg agccgttcgt gacggatttt
    10321 taacagcgat gccagctatt ttattttcaa gcattttcat tttgattgct gcggttcctg
    10381 agatttttgg ttaccaatgg cctgaatctg tctccacatg gctatggaaa gtctataatt
    10441 attcgatggg tgttgtaggg ttactcgttt cagcaaccac tgctcgctgc ttggctgatt
    10501 cgatgaatcg tcgcttaccg aaaaacaaag taatcaacac cacctctgtg atgctggcct
    10561 ctgtggtaag tttcctgttg ttaagcgtct ctccgattga aggtggattt tcaacggaat
    10621 atatggggac aaagggacta ctggcttcct ttgtcgctgc atttttaaca gttaatttat
    10681 ataagttttg tgtgctgaaa gatattacca ttaaaatgcc taaagaagtt cctggaacga
    10741 tttcgcaaac cttccgagat atttttcctt tctctttttc agtatttgcg gccgttttaa
    10801 ttgatttagg cattcgacaa atcttccaaa tctcatttgc agaagcaatc attacggtgc
    10861 tgcagccgct atttacagca gctgacgggt acttaggaat cgctattatc tggggagcga
    10921 tggcgctgtt ttggtttgtc ggtgttcatg gaccgtcgat cgtggagccg gcaattgcgg
    10981 caattatcta tgccaatgtg gaaaccaatc tgcaattgtt caattccggt gaacaagctt
    11041 ctaaagtact gacggtgggt ttagggaatt ttgtaggaac tatgggagga actggggcaa
    11101 cattggttgt tccttttatc ttcctactct ttgctaagtc taagcagttg aaggcagttg
    11161 ggaaagcttc ctttattcca gtaagtttcg ctgtcaacga accattgcta tttgcggcac
    11221 ccatgatttt gaacccttat ttttttattc catttttgtt aacacctgtt gtaaacgttt
    11281 gcctgttcaa gttttttgtc gatgtattgg gcatgaacag ctttatgtac gttttgcctt
    11341 gggcaactcc agcaccgatc ggtttggttc taggaacggg catgggtatc ttagcttttg
    11401 ttctagcagc ggttctaatc gtcgttgact tcctgatcta tctaccattt tgcaaagcat
    11461 acgatgctgt cttagtagag aaagaagcac aaaaagcaga ggtgatcgca accgaggaag
    11521 gtgcttctgc cgatgtacgt gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg
    11581 caaataaaga aatcaatgtg ttggtacttt gtgctggcgc agggacaagt gcgatgttag
    11641 ctaatgcttt aaaaaaaggt gcatctgaat cgaaagcacc gatcagtgca atggcgggtg
    11701 cttatggttc tcattacgaa atgctgcagg attttgatat ggtggtctta gcaccgcaag
    11761 tcaattctta ctatgatgat atcaaagaag acaccgatcg cttaggaatc aagctggttg
    11821 cgacaaaagg agcagaatat atcggattaa cgagagatcc tgaaaaagct gttcagttta
    11881 ttctgaacca atttcaataa aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa
    11941 gctgttttat ttgcagctgt gttaggggct ttgggggctg tttcaaaaac tgttgaagca
    12001 tctgccagcg aatttattcg cggagcagat atttcgatcc tgtcagatat ggaaaaatct
    12061 ggtgcaaaat attatgaaga cgatatttca aaagatgcct tagagatttt aaaaaataat
    12121 ggtgtcaact atgttcgact gcgtttgtgg caggatcctt ataatagtaa tggagaaagt
    12181 tatggagcag ggacaaatga tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat
    12241 ctgggactta aggtgctgct ggattttcac tacagtgatt tttgggttga tcctgggaaa
    12301 cagaatcttc ctaaagcatg gcaaggtctg acctttgaag aaatgaatac ggcattgtac
    12361 gactatacaa agaacgttct gtcggaaatg aagcagctgg acgtttatcc tgatatggtt
    12421 cagatcggca atgaactgaa tagcggaatg ctctggcctt atggcaagtc ttggggagaa
    12481 ggcgggggag aatttgatcg attggctgct tttttgaagt ctggtatcca agctgttcga
    12541 gacactcaac ctaaaacaac tccagtaatg ctccacttag ctgatggtgg cgatacagga
    12601 gcatttactt ggtggtttga tgaaatcacg agtcgaggtg tgtcatttga tttaattgga
    12661 gtgtcgtatt atccgtattg gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg
    12721 atcagccaac ggtatggtaa agaagtgatc gtagttgaaa cagcttatgg gcatacaaca
    12781 gcgaatgccg atactatgcc aaatgctttt ggtgaagccg aagcagctgc ggggggatat
    12841 gaaccaacac cgacaggaca agcagagtat ttgctggatc ttgccgatcg gattcaggca
    12901 gtaccgaata atcgcggggc gggcttcttc tattgggaac ctctttggta caacggaaat
    12961 gtgagttggg caacacaagc aggtatgaat tatctcagtg ttcaatccac tatgggaaac
    13021 gaatgggaca accaagcgat gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg
    13081 tttaaacaag caggggcgca aataaatcta gttaaaaatg caagtttcga aagcgatgga
    13141 ccaacgacca gtccttccaa ttggcaaacc tggtttcaac aaggaaccgc taagggtacg
    13201 atcaaaacgg aagcgggcac tttttttggg gactacaaac taactttttg gcaggatcaa
    13261 gcatacgagg catcagctta tcaaaatgtt agtggcataa gcaaaggaac ttacacattg
    13321 agtgcctatg taatgtctgg gggagagttt gatactaatc agttatatat caaaaattat
    13381 ggtggagcgg aactaaatac tgcggttgtt aaaacaagtg aatggacaaa aattacaatc
    13441 aaagacattc caattacaac aggtgtttgt gaaattgggg tttatagcaa ggcttcagga
    13501 aatgcttgga cgaacgttga ttttgtttca ttggtgaaac aatagactgc ggatcgtttc
    13561 gattcaagat cggaggttcc cttcagccac atcgttagcg tagaaggcag gaatcgaacc
    13621 tgcacctcgc ctttgccaaa aataaccaga aacgatttcc agtcattttt accaaaacga
    13681 gtaaagcacc agacttcatc tacactttgt attatgctaa agagagttca acgatttcgt
    13741 aagaagcaat gaaaaaattg caaaaagttg tgcagataac ataaagtttg tttaagaaag
    13801 ggtaacctta gaattccgtc gtgaatatga tttgagcact tagatggata gaaaaaagtt
    13861 ctcggaaaac agttcgtttt tcgagaactt ctttttatga taagaatgaa acaaattaac
    13921 ttatattata gtctcttttg gtagggaacc atcttatttt taatgaaaat caagcatttt
    13981 agctattgaa gaatgttgat ttatagggat tccccctcca tattatgttg gctctttatt
    14041 cccactccac tgttgctggt ggtttagaag tgatgtcata cacgatacgg ttgacgtggg
    14101 ctacttcgtt tacgatacgt acagagattt tttgcaagac ttcccatggg atacgggcaa
    14161 agtcagcggt cattccgtcg attgaagtta cagcacggat accgactgtg tagtcgtaag
    14221 tacgtccgtc acccataaca ccgacagagc ggatccctgg aagaacagtg aaatattgcc
    14281 agatatcacg gtcaagtcca gcagctgcga tttcttcacg caagatagca tcagattcgc
    14341 gtacgatttc tagcttgtct tcagtgattt cgccaagaac acgaatacca agaccaggac
    14401 cagggaatgg ctgacgccaa acgattgaat caggcatgcc taactgagtc cctaattcac
    14461 ggacttcgtc tttgaataat gtgtttaatg gttcgatcaa ttcaaattgc atatcttctg
    14521 gcagtccacc tacattatgg tgagatttga tcgtctgtgc tgtttctgta ccagattcga
    14581 taacgtcagt atacaacgtt ccttgtgcta ggaaagagac gccttcttca ccagcaagtt
    14641 ttgttgcttc gtcatcaaaa acatatacga attcattccc aatgatttta cgtttttgtt
    14701 ctgggtcaga tacacctttc aacttgtcta agaagcgatc tcttgcattg actttgatga
    14761 tgttcaagcc gaattttccg cctagacttt ccattacttg ttccgcttcg cctttgcgta
    14821 gcagaccgtg gtcaacaaag atagatgtca attggtcacc gatcgctttt tgtaaaagaa
    14881 ccccaacgac actagaatca acgccacctg aaagaccaag caagactttt ttgtcaccaa
    14941 cttgttcacg gattttttct acttgcatat cgataaagtt atccattgtc cagttacctt
    15001 cacagtgaca aacatcaaag gcaaagtggc gcagcaattc gattccgtat tcggaatgac
    15061 gtacttctgc atggaattgg ataccataaa aattacgtgt tgtgtcttgg atagaagcaa
    15121 tcggacaatc tttgcttgtt ccgactgttt caaagccttc aggaacttta gttaccaagt
    15181 caccatggct catccatact gtttgttgtt ttggtgtttc tttaaacaag acagcttgat
    15241 cgcttgttac ttctaacaag gctttcccat attcgcggtt tttagcaggc tctacggaac
    15301 cgccaagttt gtacgtgatc aattgcatac catagcaaat ccctaataca ggaataccta
    15361 aattgaagat ctcaggatcg atcccaaatg cattctcatc atatacactg tttggtccgc
    15421 cagaaagaat gattcccttt ggattcatcg cttttacttc ttctgcagta atgcggtggc
    15481 tcaataattc ggaaaagact ccaagatcac gaatacgtct tgtgatcaat tggttatact
    15541 gactaccgta gtctaatacg ataatctttt caacgttcgt catatcggca acgtttgtca
    15601 aatttattac ccctatccct ttaaatttta tctcaaaata gtcaaactat tttaaaatgc
    15661 taatattttc gcatgaaaat cgaatcgatg atatgatttt cagttttatg gagaattata
    15721 tcggctcttc ctcgtgtagg caagatatat tcctgtaaat ttttcaaatt cacatttttc
    15781 catacatctt ttgccatttt aaaggcttct tcacgatctc cgatagcaaa acgataataa
    15841 taattctctg gattttggaa agccgtatcc agtaacgcac caaaacgatc caaataccat
    15901 ttttcgatca gcacgggatc ggcatcgaca tagacagaaa aatcaaaaaa gtcgctgacg
    15961 tagatttgct gattcgcagg taattgaagc gtattgatcc cctcaacgat caagatatca
    16021 ggctgctgga tcaattcgta cttgtctgga atgatatcat acacatcatg tgagtaaaca
    16081 ggtactttta tctcttcctg accagatttt acacggttca agaaatcgat cagcatctcc
    16141 atatcataac tttcaggaaa tcctttacgg tccatgattc cacgctcttt taatatacga
    16201 tttggataaa gaaatccgtc agtcgtgatc agctgaacgt ttcttcgttt gaaggtacga
    16261 gacaaaatcg tctgcagcag tctggctgtt gtactttttc caacggctac acttccggcg
    16321 atcccgataa taaaaggcgg tacttctacg tatttatgaa gaaataaacc tttactcaag
    16381 aacaaagatt caaattcttt catataaaga taaatcagat gagcaagagg tacatagata
    16441 tctcgaacat cctgcatgga aatgcggtca ttcaaacttt tgatattatc tagttcatct
    16501 tgtgtcaaag gtgcttgccc attacgataa aattctcgcc attcttctct tgccacacgg
    16561 tagtagttca ttggatcatt catattagcc tcctgcgttc tcccatcaat ggaactattt
    16621 taacacaaaa taaacgcaaa ccccaccact taagagagat tgccgatctt tttgtataat
    16681 ctttataata gaaagaaacg gagtcttatt cttgacttgc gcacggatac ataaaaggat
    16741 agactgttgt catgaaagaa atgagaagga tggaagacat gacaaatcat tactattctg
    16801 aacgcccaga tacggcgcac gattttgaac aatgggcttt tgagttaaaa ggcagaacct
    16861 ttcaatttgt aacagattct ggtgtatttt cacgtgaaac agtggattat ggttcacgtg
    16921 tcctgattga tgctttcgat tgggaagagt tacctgatga aggaaagatc ttagatgtgg
    16981 gatgcgggta tggaccgatt gggttagctt tggcatttgc gacacagcgg tttgttgaaa
    17041 tggtcgacat taatgaacga gcggtaagtc tagcgcaagg aaacgcaaag cgtaaccaaa
    17101 tcgaacatgt tgatatccat caatcgaata tctatgaagc agttcatgaa gaaacctatg
    17161 cagctatcgt aagcaatccg ccgatccgtg ctggaaaaaa agttgtacat gaaattttga
    17221 caggcgcata tcctcggttg aagaagggcg gtacactgac aattgtgatc cagaagaaac
    17281 aaggagcgcc gagtgcgcaa aagaaaatgg aagaaacatt tggtaatgca gaaatcgtca
    17341 caaaagataa aggatattat atcatcaaaa gtgtgaaaga ataactagct gaaaaatagt
    17401 ggagcgacga aagagtggaa ctgggcgtgc atgtacgggt acgtatcagt tctttttttg
    17461 ttgagaagtt tttaaaaaca aagaactatt ttttcttcaa aaagaaatag taggaacaga
    17521 gtcgttgttt tttacgataa aataaataag atacagaact tgtatagaag gctgccaagt
    17581 cggtagataa aggaggtttt tatcaaaaaa catactttcc ttttttctct attcttttct
    17641 tgggggtttt ggtacactga tagggaattt agatagaagg gagccaatag gatgaagctg
    17701 accatcagag atatcgcaga aatggcaggc gtatcagtaa caactgtctc acagattttg
    17761 aataacaaag gaagcagatt tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat
    17821 gaacatcatt ataagcccga ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc
    17881 atcggaatga tcgtccctga tgtcactgat ttcttcttct ccaaactgat cgaaggtgta
    17941 gagagctatt tgaatccttt gggctatgtg atcatgttat gcaattctcg tcatagccaa
    18001 gaaaatgaga tcaagtattt acaagagctt tcccaccgct cagtggacgg aatcttgctg
    18061 gccacaccaa atattttacc tgagaaatat gcattagaca gcggttttta tcaaaaaatg
    18121 ccaatcattt taatcgatcg cggattgaat cgtcgggata acggacggct tatcgtaaag
    18181 gaatatgagg gcgcctatca agctgtttcg ctgtttattg aaaatggcca tacgaagatc
    18241 ggcatgctga aggaaaccac tggatactat caattagaag aacgtttcaa cggatatcgt
    18301 catgcgctga aagatcatgg cctgcctttt aatgggaaat ttgttgaaca tggagagttg
    18361 actgtccaag gtggatatac tgcatcaaaa aaattactca aacataaaga tatcacggct
    18421 attttttgcg ggaatgatgc catggcgatc ggatgctatc aggcgatcga tgaattgggc
    18481 aaaaaaatcc cagaagatat ctcagtcata ggattcgatg gattaaagct gtcagaatac
    18541 atgattccgc aattgacgac agtgcagcag ccaagttttg atattggatt ttatgctgca
    18601 cgtttcctga tcgatacgat cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa
    18661 acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa agggctaact cggcggtata
    18721 ttgcttatgt aagcatttac aaaggctagt tagtgctttt tttattttta gtaaaccagt
    18781 ttactaaagt ggtttacctt tcctaaaaat aatgtataat accgaatgaa gacaaggagg
    18841 atcaggcaat gcgaatggtg gatctgattg aaaaaaagag ggatggaaac gagctgtcga
    18901 aagaggaaat cgagtacatc gtcacgaact atacgaatgg aaagattcca gattatcaag
    18961 tcagtgcatt attgatggca attttttatc aagacatgac aaatgaagaa atcacgaact
    19021 taacattggc gattgctaat tctggtgatg tcatcgattt gagttctctt gaaggaataa
    19081 aggtagataa gcattcgaca ggtggcgttg gtgacacgac cacattgatt ttagcccccc
    19141 tagtagctag tgtcggagtg acagtggcta aaatgtctgg acgcggtcta ggttacacag
    19201 gtggtacact agacaagcta gaagcaattc ccggatttca gatcgagctt tcggatgaag
    19261 aatttgtacg cattgttaat gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg
    19321 caccggcgga caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt gattctcttc
    19381 cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt
    19441 tggatgtgac cactggagac ggtgctttca tgaagaacat cgaagatgca agacgtctgg
    19501 caaagaccat gacgagcatt gggaaactgg ctaaccgaga aacagtggcg gttatctcag
    19561 atatgtcaga acctcttggc gaagctatcg gaaatagtct agaagttgtc gaggcaatcg
    19621 aaacattaca aggaaatggt ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc
    19681 aaatggttgt tcttgctggc aaagctaaga caaccgatga agcaagaacg ttgcttcaag
    19741 aagctctgga atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa aatcaagggg
    19801 gagacccagc gatcgtggaa catccagaac gaattttgac tgcacgctac acaatggagc
    19861 ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg
    19921 caatgatgct aggtgctggg cgtaaaacca aagaagaaga tattgatcac gctgttggat
    19981 tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta ttgacgatct
    20041 atagtaacga cgaagaaatc acttctgtca ttgaattatt atataaaaat attgaaattg
    20101 gtgaatcggc aatgaagcca acgttgattc atgacatcat cacagaataa aggaaaaaag
    20161 cggactttac cgttttaaag aaaaggggaa aagaaatgga actaaaccga atgatcgacc
    20221 atactatttt gaaagcagac gcatctaaag aagctgtgat gcaaattatc gaagaagcaa
    20281 aaaaatacca tttttattct gtctgcatca atccagcatg ggttgcgcta gcgaaagaac
    20341 aattacaagg tgagccagta gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca
    20401 cacccgagac aaaagcattt gaaacaacag atgccatcaa taacggtgca gatgaagtcg
    20461 atatggtcat caatatcggt gctttgaaat caaaggatga tcaacaagtc caaaaagata
    20521 tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt attgaaacat
    20581 cgttattaga tagagaagaa atcatccgag cttgcgaaat agcaaaagct gcaggcgctg
    20641 attttgtcaa aacatcaact ggtttctcaa caggcggagc aaaggtagag gatgtccgtt
    20701 tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt atccataacg
    20761 aagaagaagc aatggcaatg atcgaagctg gtgctacaag aatcggaaca agcgcaggag
    20821 tagcaatcgt atctggttca actggtgaag gctattaatt ttttcaaagg agaagctcat
    20881 ggcagaactc aaacaagaat ggatcaacac agctattgaa gcgttagata aagcctatgt
    20941 tccttattcc cattttccgg taggcgcttg tctcgtaacg gaatcaggca agatttatca
    21001 aggcatcaac attgagaatg catcatttgg attaacgaat tgtgccgagc gcacagcttt
    21061 tttcaaagca gtatctgaag gagaaaaaac atttacgcat ctagtcgtag cggggcacac
    21121 ccccgatcct atctctcctt gtggtgcatg tcgacaagta atggcagaat tctgttcccc
    21181 tgatatgccg gttacgctag taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc
    21241 actgctgcct tatgcattta ctgaaaaaga tctataactc atgttcattc agtcagctga
    21301 ctgttgaaaa ataaaacaat tattttagga ggctttacag aaatgaaaaa agcaaaatta
    21361 tttggtttag gtgctgtcgc actagcagca gggttatttt taggtgcatg cggcaacaac
    21421 ggctctactg attcatccgg tggcaaaagc tcatcagaca caacaactgc agcattgatc
    21481 acagacacag gtggtgtaga cgaccgttca ttcaaccagt cagcttggga aggactagaa
    21541 aaatggggga aagatcaagg actttcacga ggaaatgatg gcttccaata tttccaatct
    21601 tcaaatgaat cagattacat cccaaatatt gaccaagcat tgaatgctgg attcaaaacg
    21661 atttttggta ttggttataa actaaaacca gcgatccaag aacaagcaac aaacaataca
    21721 ggtacgaact ttgttattat cgatgatgtc atcgatggat tagataacgt tgtatctgca
    21781 actttcaaag acaacgaagc ttcttatctt gcaggtgttg cagcagctta tacgactgaa
    21841 acaaacgtag tcggctttat cggcggtgta aaaggtgaag taatcgatcg ttttgatgct
    21901 ggattcaaag ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat caaagtattg
    21961 aatcaatatg ctggtgactt ctctgctcca gataaaggac gttcaattgc tcaaggtatg
    22021 tatgcacaaa acgcagatat catcttccat gcttcaggcg gtacaggtaa cggtgtattc
    22081 caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag tttgggttat cggtgttgac
    22141 cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg
    22201 acatctacac tgaaagcagt tggaacagta gtagaagatt tggcacaaaa atcagcggac
    22261 ggtaaattcc ctggcggcga acacactgtt tatggattga aagaagacgg tgttggtcta
    22321 acagaaggac aactttctga cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc
    22381 atttcaggtg acgtaaaagt tccagaaact cctgaagaaa actaaaacca tttctatagc
    22441 cttgttagac acatgtttga caaggctatg gtttgattag aacaagaatt gttcatttat
    22501 ccaagctgcc ttgtgacagt ttggataagg gaacaactcc ctgaagtaat gtttgtttca
    22561 aaagaagaac gctcactaag cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt
    22621 aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga atattaccaa
    22681 agaatttgga acattcaagg caaatgacaa catcaattta caagtaaagg caggagaaat
    22741 tcatgcgttg cttggagaaa acggtgctgg caaatctaca ttgatgaacg tgctttccgg
    22801 attattagag ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag
    22861 tccgaccaaa gccaaccaat tagggattgg gatggtccat cagcacttta tgcttgttga
    22921 tgcctttact gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg cagggatgct
    22981 tgaccataaa aaagcacgaa aagagatcca aaaagtttct gaacagtatg gattgtcagt
    23041 caacccagat gcttacgttc gtgatatttc agttggaatg gaacaacggg tagaaatttt
    23101 aaaaacactt taccgaggag cagatgtact gatttttgat gagccgacag ctgtattgac
    23161 ccctcaagaa attgatgaat taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc
    23221 gatcattttg attacgcata agttagatga aatcaaagca gtagctgacc gctgtacagt
    23281 tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct cacaacaatt
    23341 agccgatatg atggttggaa gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc
    23401 tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc
    23461 cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta cttggtatcg ctggaatcga
    23521 tggaaacggg cagtcggagt tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg
    23581 acatatcaag ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa tcactgaaca
    23641 tggtgtagga cacgtgccag aagaccgtca taaatacggg ttagtcctag atatgacatt
    23701 atctgaaaat atcgcactac aaacctatca tcaaaaacct tacagtaaaa acggcatgct
    23761 gaattattca gtaatgaatg aacatgccag agaattgatc gaagaatatg atgttcgaac
    23821 aacgaatgaa cttgttcccg caaaagcttt atcaggcgga aatcagcaaa aagcaattat
    23881 cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt gcaaatccaa ctcgtggact
    23941 ggatgtaggt gcgatcgaat ttattcataa acgtctgatc gaacaaaggg acaaatacaa
    24001 agcagtgtta ctgatcagtt tcgaattaga agaaatttta aatgtttcgg atcgtattgc
    24061 tgttatccat gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga
    24121 attaggtcta ttgatggcgg gctatacctt agaagaagca cgaaaagagt tagaaaaggg
    24181 ggctggcgca catgaatgat cgcagcgaaa aattccgtaa tttattagtg cctgtacttt
    24241 cagttgtttt aggattgatc ttaggcgcga tattgatggc tgcttttggt tttcagccca
    24301 tccaaggtta ttcttctatg ctagatgcag cccttggttc gcaaagaggg ataggtgaaa
    24361 cgttaaggca agcaacgccg ttgattttta cagcattagg tttttctgta gctaactcag
    24421 ctgggttttt caatattggg ctttctggac aagcactttg cgggtggatc gtcagtgtct
    24481 gggtggcact tgcgtttcca gatctgccaa aagttgtttt gcttccgtta tgcgtgatct
    24541 taggtgcctt agctggtgcg atggcagcgg ctgttccagg tttacttcga gcattttttg
    24601 gaacaagtga agtgatcgta acaatcatga tgaactatat cttattatac gtaagcacat
    24661 ttgctttaca tgaagtgatg cctgaaagct atcgctcaag tttggactct tccaatatga
    24721 tcagtcaaaa tgcctcactg cggttaccat ggttaacaca agcttttggc ggctctcgcg
    24781 taaactcagg attattttta gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga
    24841 aaacgacttt aggatttgaa atccgttctg ttggattgaa tccttttgct tctgagtatg
    24901 caggaatgag tagtaaacgt acgatcgttt tgtcgatggt catttctggc gctttggcag
    24961 ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt caaacgactt
    25021 cgctatctat tggatttgac ggaatggctg tttccttgct tggttctgga tcagcaatcg
    25081 gtattctttt atcagcgatc ttgttcagta ttttgaagat cggtggactt ggcatgcaga
    25141 caggagcagg tgttccgttt gagatcgtca atgtatcgat tgctttgatt atcttctttg
    25201 tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag
    25261 aagagatcgt tgcgacaata gaatcgaaac caacgaatca aaaccatgaa gggggcgaac
    25321 tataatgtcg actatcgaat taattgcctt gatccttact tctacactgg tttattcaac
    25381 accgctaatt ctaacgtcac taggcggaac tttttctgaa cgaagcggga tcgtaaacgt
    25441 agggctagag ggaatcatgg tcatgggtgc ttttagttcc atcgttttca acttgacgat
    25501 gagtccatca cttggttcta tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg
    25561 tatcctattt tctctgattc acgcagtagc aacagtcagt ttgcgtgctg accatattat
    25621 cagcgggaca gtaattaatc tgatggcacc tgctttaggt gttttcttga tcaaagcatg
    25681 gtatggtaaa ggtcaaactg ataacatcac gcagaatctg gggtattttt ctttcccagg
    25741 attagcagat attccggtca ttggaccaat tttctttaaa aatacattct tacctgctta
    25801 ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc ttcaagacga aattcggctt
    25861 gcgcctccgt tcagttggtg aaaatcctca agctgccgac acattaggta tcaatgtata
    25921 tgctatgcgt tattcaggcg tactgatttc tggcttgtta ggcggtatgg gcggtgctgt
    25981 ttttgctcag tcgatttccg gtaacttttc agctggtacg atcgttggac aagggttcat
    26041 ctccatggct gctatgatct ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt
    26101 gttctttggt tttgctcaaa gcttgagtat cattggcgca caattaccag ttatttcttc
    26161 gattccgcca gtattgctgc aaattgctcc atatttgctg acaatcattg tgctggttgt
    26221 tttcttaggg aaagcttctg gtccaaaagc aaacgggaag aactatatca aatcaaaata
    26281 atcttttagc gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt
    26341 tttaaaggca ataaaaataa aaagaaagaa ggaataatca tgtttaaacg cgtacatctg
    26401 atcgtcatgg attcagtagg aattggagaa gcaccagatg ccgataagtt cggtgatgtt
    26461 ggttctgata cattaggaca cattgcgaaa gaagcaggat tgaacgttcc acatttagag
    26521 cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa
    26581 ggctatgcga cgaaattaga agaagtttct gtagggaaag atacgatgac aggccactgg
    26641 gaaatcatgg gattgaatat caaaactcca tttcgtgtgt ttcctaatgg attccctgat
    26701 gatctgatca aacaaatcga agaattttct ggccgtaaag tcgtatgtaa caaaccttat
    26761 agcggaacag ctgttattga cgattacggt gaacaccaaa tgaaaacagg cgatttaatt
    26821 gtatatactt ctgccgatcc tgtccttcag atcgcggcac atgaagaaat cattccttta
    26881 gaagaattat atcggatttg ccaattcgtt cgtgatataa caaaagatga tccgtatatg
    26941 attggccgaa tcattgcacg tccgtacgtg ggtgaaccag gagacttcac acgaacaagc
    27001 aatcgccatg attatgcgtt gaatccattc ggacacacag tccttgattc tttgaaagaa
    27061 gcaggaaagg acgtgattgc tgttggaaag atcaacgata ttttcaacgg acaaggaatc
    27121 actgaatctg tccgcacgaa aagcaatatg gatggcgttg accagctgct gaaagttatg
    27181 aaaaacgatt tttcaggaat cagctttacc aatttagtag attttgatgc attatacggt
    27241 catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag aatttgactt acgaattcca
    27301 gaattgcttg aagcgatgga agaagacgac ttgctaatga ttacagccga tcacggaaac
    27361 gatccgacgt ttactggtac cgatcatacg cgagaatatg taccgttatt ggtttacagt
    27421 aaaaaaatga aagaacaagg ggcattgcct caaggatatt attcagatat ttcagcaacg
    27481 attgctgaga actttagtgt ttcttcgact gaaaatggcg aaagcttctt gaaattatta
    27541 aaataatctt tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa
    27601 cgacccagtt tatccttgat aaaggagtaa aagaaattga ttttggtttg attctaggtt
    27661 caggattagg tgaattagca gatgaaatcg aagatgctgt aatgatttct tatgatgaga
    27721 tcccgttctt tcctgtttcg acagtagttg ggcatgcagg aaagctagtt tatgggtctc
    27781 tggcaggcaa gaaagtgtta gccatgcaag ggagattcca ctactatgaa ggtcattcca
    27841 tgcaaacagt gacctatcca gtacgtgtga tggccggact aggggcccat tcagtcattg
    27901 tgactaatgc atgtggtggt gtcaacgaat catttgtacc aggagatttg atgctgatta
    27961 ccgatcatat caattttact ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg
    28021 gccctagatt tccagatatg agtcaagctt atacgcctga ctatcaagca acagcaaaag
    28081 cagcagcaga aaaactaggt ttggatctta aagaaggcgt atatatgggc tattctggac
    28141 caacttatga aacgcctgcg gagatccgaa tgtctcggac aataggagcc gatgcagtag
    28201 ggatgtcgac tgttccagaa gtcatcgtag cagctcacag cggattaaaa gtgttaggaa
    28261 tctcttgtat taccaactta gcagcaggta tgcaagcgaa tctgagccat gaagaagttg
    28321 tagaaacaac tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa acattgacat
    28381 tattgtaata ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga aggcgagatc
    28441 gccgataaga ttttacttcc gggagatcct ttgcgagcta aatatattgc ggagactttt
    28501 ttggaagatc cagtttgtta caatcaggta cgcggtatgt taggctttac cggcaaatat
    28561 aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc caccatctat
    28621 gcccatgaat tgatccagtc gtatggtgta aagaaattga ttcgagtagg tacatgcggt
    28681 gcattgtcaa aagacgtcca tgttcgtgat ttagtactag cacaaggtgc agcgaccagt
    28741 tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag tgatttcaat
    28801 ctattactga aagcatatga gatagctaaa gaaaagggct atacggttca tgtaggaaat
    28861 gttctttcgg aagattcttt ctacaaagat gatttgacag agacgttcca attagctgaa
    28921 cttggcgttt taggagtgga aatggaagca gcggctcttt attacttagg cgcgaaatac
    28981 catgtgcaga cattaagttt gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca
    29041 acagctgcag aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg
    29101 atagcagaat aagaaggaaa tgaaaaagct gccctgcatt aagtaataag aacatccaag
    29161 caaaaatagc tcctcatgtt tttgacttgg ctggacttat tattgcaaat gtaagctttt
    29221 gaacaccgtt taccaagagg ttgtgaatct gccgtttaac tctgagtcac aaggaacaat
    29281 ttcaaaaaac agcttttcat gtttgatgaa tattgtgact tgtgaccgag aagttggcag
    29341 gagaaaaccg tttatacgaa aaaagggact gtgacaaatc gtgtcacagt ccctttttta
    29401 tagtgttttt tataggtagt attttttagc aactgttaag tcatcattta attcataaac
    29461 aagtggttga cctgttggaa tttcaagatc catgatgtct tcgtcagaga taccttcgat
    29521 atgtttagct agggcgcgta atgagttacc atgtgcagct actaaaacag ttttgttgtc
    29581 tagtaatgct ggagcaattt catcttgcca gaatggcaaa gcgcgttcta atgtcacttt
    29641 caagttttct ccaccaggaa tatcgcgttt gtctaacatt gcgtaacgac ggtcatttgc
    29701 tgcagaacct tcgtcagttt cttccattaa tggaggaaga gtatcgtaag aacgacgcca
    29761 gatgtgcact tgttcgtcgc cgtatttttc agctgtttct tttttgttca aaccttgtaa
    29821 tttaccatag tgacgttcgt ttaagcgcca tgatttgatt tgtggtaccc aaagttgatc
    29881 tgaatattct aagatcaagt tacatgtttt gattgcacga gttaaaacag aagtgtaagc
    29941 aacatcaaat tcgattccag cttctttgat tttgcgtccg ccttcttttg cttcttcgat
    30001 tccttctggt gctagattta catcagccca accagtaaat tggttcaatg cattccattc
    30061 actaagaccg tgacgagaaa aaactaattt tggcatgttt agctctcctt ttaagatagg
    30121 tttatatttc tgaaattttc agttctgttt tattgtacaa ttttttggaa gagatttcca
    30181 tcgaaaaatt agataagatt ctatgagaaa acaagtagag aatataaaaa aacgaacagt
    30241 gcccacgcaa ctgttcgttt ttaggtatta gatgcataat tgctcgattt ctttttcagg
    30301 taaattgata tatttataac atgttccaac tgaaacaccg cattttgaag aaataaattg
    30361 gatcgtttct tttttttcat aatagagacg tcggattttt ttgatcgttt ttgcatcgat
    30421 ttttggtctt ccgccgattt ttcctttttt acgagcttca tctaatccaa ctaatgtacg
    30481 ttctttgatc aatgcgcact ccatgtttgc taggttttcc attaattgga aatagatttt
    30541 tcccatttcg cgcgtatcga ttccttcatc aagactaatc aagtgcagcc cgcgatcttt
    30601 gaataattgc gtgagttctg ttaattgccg cgtagatttg cctaatcgat gaagttggca
    30661 aatgactagt gcatcgcctt gttgcatgtc agcgatcact gactccaaat gaacagcagt
    30721 agaatcattt tgttccactt catatgtctc atggaaaatt tcttgacaac caaagtcaga
    30781 taattgatca acctgagttg ttaaatcaac gtcatttatg gttgtgcgtg cataaccaat
    30841 aattttcatg gtgattcctt tctagcagtg attttctaac tggatataac agtaaaaatg
    30901 tagcataaag tacgtcaaaa agatagtttt ttcgcaaatt ttccgctgtc tttttttatt
    30961 ggatgatcaa gcagtttttc tttatttacg acggattgcg tcaagaatct caacgccttt
    31021 ttctgagccg acaactactt tagaaaccat gtttaggaaa agcccatgtt ctactacccc
    31081 aacaagttca tctaaatagg tagctaagct ttctggctgt tcgatgactt ctagatgcag
    31141 atcaatgatg taatgaccac catctgtggt taagacttct ccgttttcgt ctgtacgcaa
    31201 gacgggttgg aatctttttt cttcaaaaat atgaagtagc tgttgggagc cataagggat
    31261 cacttcaact gggagtggga acttcccaag tttatcgact aatttagaag aatcaacgat
    31321 ccaaatcgtt tcttttgagt acgtagcgac tattttctcg aaaagcaaag cagcgccgcc
    31381 gcctttgatc ccttgaaaat cagcactgat ttcatcagca ccgtctattg tcaaatcgac
    31441 tacggggact tcatcaatac tctttaacgg tattcctaat ttttccgctt gttcttttgt
    31501 cgcatttgaa gtagtaactc ctgtgatacg taaaccttct tcacgcatac ggcgaccaat
    31561 ctcttctacc atataatagg cggttgaacc ggtcccgaga cctacgatca tcccgtcctt
    31621 tacgaatttc gcggcttcaa tgccagctaa ttgttttaaa ttcatctttg cgtccacctt
    31681 tttttagttt gagcatcgtg ctttcctcac tattttagcg tgatttacgg taaaaagata
    31741 ccattctttt ttattccaat aaagcttttt caaaaaaaat tgcatctctt gttgacatgt
    31801 tttttcattc gtgatattct aacaaaggtg ctttatctac aggtttctgt gaagaaacgt
    31861 gctgactgtt tatcaaagca tatttgataa ggatgcagac attgcattta ttttttatca
    31921 caaaaaaaga accacctgga tgtgtggaac tagacgcaaa tcgtgggaag gaggaaacaa
    31981 ggaatgccta caattaacca attagtacgt aaacctcgta aatcaaaggt ggaaaaatct
    32041 aattcacctg ctttgaacaa aggatataac agctttaaga aaactcaaac taacgtaaac
    32101 tcacctcaaa aacgtggggt agcaactcgt gtgggtacaa tgacacctaa aaaaccgaac
    32161 tcagctttac gtaaatatgc acgtgttcgt ttgtcaaact taattgaagt tactgcttat
    32221 atcccaggga ttggtcataa cttacaagaa cacagtgttg tattattacg cggtggtcgt
    32281 gtaaaagact taccaggggt acgttaccat atcgttcgcg gtgctttaga tactgctggt
    32341 gttaacgacc gtaaacaaag ccgttctaaa tacggaacta aaaaacctaa agcttaattt
    32401 taattattaa acaaatgaaa cttgcttcta ctatatagaa gtaattacta tgaacaatat
    32461 ttcggaagga ggagttacgg atgccacgta aaggtcctgt tgcaaaacgt gatgttttac
    32521 cagatcctat ttataactca aaattagtaa ctcgcttgat caaccgtgta atggttgacg
    32581 gaaaacgcgg gattgcagct aacattatct ataatgcctt tgacttgatc aaagaatcta
    32641 caggtaacga tccattagaa gtgtttgaac aagcaatgaa aaacgttcaa cctgttcttg
    32701 aagttaaagc acgtcgtgtt gggggttcaa actaccaagt accagttgaa gttcgccctg
    32761 aacgccgcgt tactctaggt cttcgttggg tagtaaacta cgctcgcttg cgcggtgaac
    32821 acacaatgga agaacgctta gcaaaagaaa tcatggatgc tgcaaacaac acaggtgctt
    32881 ctgttaaaaa acgtgaagac actcataaaa tggctgatgc caaccgtgcg tttgctcact
    32941 atcgctggta agattctctc tgtttggtcg gcttttataa gtcgacctca gatagtgtcc
    33001 ttatttttag caactagata gtaaagagag gagaaacaag gagaatgacc agagaatttt
    33061 ctttacaaaa cactcgtaat atcggtatca tggcccacat cgatgctggt aaaacgactg
    33121 ctactgaacg tatcttgtac tatactggta aaatccataa aattggtgaa actcatgaag
    33181 gagcttcaca aatggactgg atggaacaag aacaagaacg tggtattacg atcacttctg
    33241 ctgctactac agcacaatgg aagggtcacc gaatcaacat catcgacaca ccaggacacg
    33301 tagacttcac agttgaagtt gaacgttcat tgcgtgtact tgatggtgct gtaactgttc
    33361 ttgatgcaca atcaggtgtt gaacctcaaa ctgaaacagt ttggcgtcaa gcaacaactt
    33421 atggtgtacc tcgtatcgta tttgcaaaca aaatggacaa gatcggtgct gacttcttat
    33481 attcagtatc aactttacat gaccgtttgc aagcaaacgc tcacccaatc caattaccaa
    33541 ttggtgcgga agacgacttc acaggaatta tcgaccttgt aacaatgaag gctgaaatgt
    33601 atacaaacga tcttggaaca gagatcgaag aaacagaaat tcctgaagaa taccgcgaat
    33661 tagctgaaga atggcgcgaa aaattggttg aagctgtagc tgaaactgat gaagaactta
    33721 ctttgaaata cttagaaggt gaagaaatca ctgaagctga gttaaaagaa ggtatccgcc
    33781 gcgcaacagt taacgtagaa ttctacccag tactatgtgg ttcagcattt aagaacaaag
    33841 gggttcaatt actattggac gctgttcttg attacttgcc atcaccgtta gatatcccag
    33901 ctatcaaagg gattgaccct aaaactgatg aagaagtaga acgtcctgca gacgacagcg
    33961 ctcctttctc agctttagca tttaaagtta tgactgaccc attcgtaggt cgtttaactt
    34021 tcttccgtgt gtactctggt gttttacaat caggttcata cgtacaaaac gcaacaaaag
    34081 gcaaacgtga acgtgttggt cgtatcctac agatgcatgc caactctcgt tcagaaatct
    34141 ctgaagttta cgctggagat atcgctgctg ctgttggttt gaaagacaca actactgggg
    34201 acacactatg tgatgaaaag agtctagtta ttcttgaatc catggaattc ccagaaccag
    34261 ttatccaagt cgctattgaa cctaaatcaa aagctgacca agataaaatg ggtgtggctt
    34321 tacaaaaact atctgaagaa gatcctactt tccgtgctga aacaaacgtg gaaacaggcg
    34381 aaacaatcat cgctggtatg ggtgaacttc acttagatat catcgttgac cgtatgcgtc
    34441 gtgaattcaa agttgaagct aacgtaggtg cgcctcaagt atcttaccgt gaaactttcc
    34501 gtgctggcac tcaagccgaa ggtaagtttg tacgtcagtc tggtggtaaa ggtcaatacg
    34561 gtcacgtatg gattgaattt acaccaaatg aagaaggtgc tggtttcgaa tttgaaaatg
    34621 cgatcgtcgg tggggttgtt cctcgtgaat acattccagc tgttgaaact ggtttgaaag
    34681 atgctatgga aaatggtgtc ttagctggct atccattagt agatatcaaa gctaaacttt
    34741 acgatggttc ataccatgat gtcgattcaa atgaaactgc cttccgtgtt gctgcttcta
    34801 tggcattacg tgcagcagct aagaaagcta atccagtaat ccttgaacca atcatggctg
    34861 tagaagttgt tatccctgaa gattatttag gtgatgttat gggtcacgta actgctcgtc
    34921 gtggacgtgt tgaaggtatg gaagctcgcg ctggcggcca acaagttgtt cgtgcgatgg
    34981 ttccacttgc tgaaatgttt ggttatgcta caactcttcg ttctgctact caaggtcgtg
    35041 gtacatttac aatgacattt gaccactacg aagatgtacc aaaatcagta caagaagaaa
    35101 tcattaagaa aaacggcgga aaagctgaat aatttcttaa aaccggatag tcctgcaaag
    35161 agattatcca aagagactat atttttaata tagtttcgtg taaaataatt atcgatgata
    35221 tgggcatgtg gtgtaccttg tgtacatcat atacctatca gacaaataaa aaatatattg
    35281 tatttttagg aggacatttt aaaatggcaa aagaaaaatt tgaccgttct aaaccacacg
    35341 ttaacattgg tactatcgga cacgttgacc atggtaaaac tacattaaca gctgcaatca
    35401 caactgtact atctaagaaa aacggcggcc aagctatggc ttacgatcaa atcgatggtg
    35461 ctccagaaga acgcgaacgc ggaatcacaa tctcaacagc tcacgttgag tatgaaacag
    35521 atacacgcca ctacgctcac gttgactgcc caggacacgc ggattacgtt aaaaacatga
    35581 tcactggtgc tgctcaaatg gacggagcta tcttggtagt ttctgctgct gacggcccaa
    35641 tgcctcaaac tcgtgaacac atcctattgt ctcgtcaagt tggtgttcct tacatcgttg
    35701 tattcttgaa caaagtagac atggttgatg acgaagaatt actagaatta gttgaaatgg
    35761 aagttcgtga cctattaaca gaatacgaat tccctggtga cgatgttcct gtagttgctg
    35821 gatcagcttt gaaagctcta gaaggcgacg cttcatacga agaaaaaatt cttgaattaa
    35881 tggctgcagt tgacgaatac atcccaactc cagaacgtga caacgacaaa ccattcatga
    35941 tgccagttga agacgtgttc tcaattactg gacgtggtac tgttgctaca ggtcgtgttg
    36001 aacgtggaca agttcgcgtt ggtgacgaag ttgaagttgt tggtattgct gaagaaactt
    36061 caaaaacaac agttactggt gttgaaatgt tccgtaaatt gttagactac gctgaagctg
    36121 gagacaacat tggtgcttta ctacgtggtg ttgcacgtga agacatccaa cgtggacaag
    36181 ttttagctaa accaggtaca atcacacctc atacaaaatt ctctgcagaa gtatacgtgt
    36241 tgacaaaaga agaaggtgga cgtcatactc cattcttcac taactaccgt ccacaattct
    36301 acttccgtac aactgacgta acaggtgttg ttgaattacc agaaggaact gaaatggtta
    36361 tgcctggcga caacgtaaca atggaagttg aattaatcca cccaatcgct atcgaaaacg
    36421 gtactaaatt ctcaatccgt gaaggcggac gtacagttgg tgccggtgtc gttacagaaa
    36481 tcaaagctta attcacttaa gttttaaagt aaagcaacat ttattcggac gtaatcgctc
    36541 ggaacgaagg tcttaggatc tttgttccga gcttttttat ttattgcagg atcaggaaaa
    36601 aagacaggag tattataaat agaagaaaca cgaaataaaa gagtcagtat tttctaaatg
    36661 aaactttatg aaaacaaaat gttttttaca agaaaaagcc ttgctttgtg agtttatata
    36721 tattataatg acaaaggtgc gtttacttta gtgaattgca cagacgtaat cagaatgatt
    36781 tcgtcggcta agaaacgaga ggttgcgaca cgcccggacg ctttgccacg aacgagcgtg
    36841 acggaaattt tcgtggagct atgtctactt ttcaaaatag gcgaaggagg gaacaagatg
    36901 gcaaaacaaa aaattcgtat ccgtttaaaa gcgtatgaac accgtatttt agatcaatca
    36961 gcggataaaa tcgtggaaac tgcaaaaaga actggagcta gcgtgtctgg tccaattcca
    37021 ttaccaactg aacgcagtct ttacacaatc atccgtgcga ctcataaata caaagattca
    37081 cgcgaacaat tcgaaatgcg tacacacaaa cgtcttatcg acattgtgaa cccaacacca
    37141 aaaactgttg atgctttgat gaagctagac ttaccatctg gtgtaaacat cgaaatcaaa
    37201 ttataaaact aaaacaaaaa taatggaggt gtactcatga ccaaaggaat cttagggaaa
    37261 aaagtgggaa tgacacaaat cttcactgaa tctggtgaat taattccagt aactgttgta
    37321 gaagctactc caaacgtagt actacaagtg aaaacaatgg aaacggatgg ctacgaagct
    37381 attcaagttg gttaccaaga tatgcgtgaa gttttatcaa acaaacctgc gaaaggtcat
    37441 gttgcaaaag caaacacggc tcctaagcgc ttcattcgtg aattcaaaaa tgttgagcta
    37501 ggagaatatg aagtaggaaa agaaattaca gtagatgttt tccaagcagg agacattatt
    37561 gatgtaacag gtactacgaa aggtaaagga ttccaagggg ttatcaaacg ccacggacaa
    37621 agccgcggac caatggctca cggttctcgt taccatcgtc gtcctgggtc aatgggtcca
    37681 gttgcaccta accgtgtatt taaaaacaaa cgtcttgctg gccgtatggg tggcaaccgc
    37741 gtaactatcc aaaacttgga agttgtacgt gtcgatgcag aaagaaatgt tatcttgatc
    37801 aaaggtaatg ttcctggagc taaaaaatct ttaattacta tcaaatcagc tgtgaaagct
    37861 aaataattgt aggaagaaag gaggaactaa ggaatgccga atgtagcatt attcaaacaa
    37921 gatggaagcc aaaacggtga aatcacactt aacgaagaaa tcttcggaat tgaacctaat
    37981 gaaagtgttg tttacgatgc aatcgtaatg caacgtgcgt cattaagaca aggaacacac
    38041 gcggttaaaa accgtagcgc tgttcgcggc ggcggacgta aaccatggcg tcaaaaagga
    38101 acaggtcgtg cacgtcaagg atctatccgc tcaccacaat ggcgtggagg tggcgtagtc
    38161 tttggaccaa caccacgttc ttacagctac aaacttccta aaaaagttcg tcgtttagca
    38221 atgaaatctg tattgtcaga aaaagttgct gaaaacaact tggtagcaat cgaaggcttg
    38281 aacttcgacg caccaaaaac aaaagaattc aaacaagttc ttgctaactt atctatcgac
    38341 tctaaagtat tggttgttct agaaaaaggt aacgactttg ctgctttatc agcacgtaac
    38401 ttaccaaacg tttctgtagt aacttctgat aatgtgagtg tgctagacgt tgtatcaaat
    38461 acaaaagtgt tagcaacaca aactgctctt actcaaattg aggaggtgct tgcataatga
    38521 acttactaga cgtaatcaaa cgcccagtga tcactgaaaa atcaatgctt gccatggatg
    38581 acaagaaata cacttttgaa gtggacactc gcgcaaacaa aactttagta aaacaagcgg
    38641 tagaagctgc ttttgacgtt aaagtcaaaa acgtgaacat cgtgaacgta cgtgcgaaat
    38701 tcaaacgtat gggcaaatat gcaggatata caaaaaaacg tcgcaaagcg atcgttacat
    38761 tgactgaaga ttcaaaagaa atccaattat tcgaagctgc tgaataagca ctaatagata
    38821 actcattaaa ttaggaggga aaaacgtggc gattaaaaag tacaaaccta cctcaaatgg
    38881 tcgtcgtaac atgacaggtt ctgattttgc tgaaatcaca acatcaacac ctgaaaaatc
    38941 attgttacaa ccattgaaaa accatgctgg acgtaacaac aacggccgca tcactgtacg
    39001 tcatcaaggt ggcggacaca aacgccaata tcgcgtgatc gacttcaaac gtaacaaaga
    39061 taacgttgtt gcaactgttc aaacgatcga atatgatcca aaccgttcag ctaacatcgc
    39121 gttagtacac tatgaagatg gagtgaaagc ttacatctta gcacctaaag gactagaagt
    39181 gggtatgaaa cttgtttcag gtccagaagc agatatcaaa gtcggaaatg ctcttccatt
    39241 agaaaacatt ccagttggta ctgttatcca caacattgaa atgaaacctg gtaaaggtgg
    39301 acaattgatc cgttctgctg gtacaagtgc tcaagtactt ggtaaagaag gcaaatacgt
    39361 attgatccgc ttgaactctg gtgaagttcg tatgatctta gctgcatgcc gtgctacaat
    39421 cggttctgta ggtaacgaac aacacgaatt aatcaacatc ggtaaagctg gacgctctcg
    39481 ttggatgcgt aaacgcccaa ctgtacgtgg tagcgtaatg aaccctaatg atcacccaca
    39541 cggtggtggt gaaggtaaag ctcctatcgg acgtaaagca ccagtttctc cttggggtca
    39601 accagctctt ggttacaaaa ctcgtaacaa aaaagctaaa tcagacaaac ttattgttcg
    39661 tcgtcgcaaa aataaataat tactttgacc atgtgtcgca cattttgaga ggaggttcac
    39721 catgggtcgt agtttaaaga aaggaccttt tgtcgatgag catttaatga aaaaagttga
    39781 agctcaagca ggggttgaaa agaaaaaagt tatcaaaact tggtcacgcc gttcaacagt
    39841 tttcccaagt tttgttggat ataccatcgc agtttatgat ggacgtaaac atgtaccagt
    39901 ttacatccaa gaagacatgg taggacacaa attaggtgaa tttgcaccta caagaactta
    39961 tcgtggacat gctgctgacg ataagaaaac gaaacgctaa tttgagggga ggataaatcc
    40021 aaatggcaga acaaatcaca tcagctaaag caactgcaaa aacagttcgc acttcacctc
    40081 gaaaagcccg tttagtaatt gaccttatca gaggtaaaag tgtcgcagat gcaatttcaa
    40141 tcttgaaatt cacaccaaac aaatctgctg gaatcattga aaaagtattg atgtcagcag
    40201 ttgcgaatgc agaaaataac tttgacttag atgttgaaaa cttggtagta agcgaagctt
    40261 ttgttaacga aggacctaca atgaaacgtt tccgtcctcg tgcaaaagga tcagcttctc
    40321 caatcaacaa acgtacaagt catatcacgg tagtcgtatc agaaaaataa ggagggataa
    40381 tcagtgggtc aaaaagtaca tccaattgga atgcgtgtag gcatcatccg cgactgggat
    40441 gctaaatggt atgctgaaaa agagtatgct gagttcttac acgaagattt aagaatccgt
    40501 aaatttattt caactaaact tgctgatgct gctgtatcaa cagttgaaat cgaacgcgca
    40561 acaggtcgcg taaacatttc aattcacaca gctaaaccag gtatggttat tggtaaaggc
    40621 ggttctgaag tcgaaagcct aagaaaagaa ttaaacaaat taactggtaa aagaatccat
    40681 atcaatatcg tggaaatcaa aaaaccagat ctagatgcaa aattagtagg tgaaggaatc
    40741 gcacgtcaat tagaaaaccg tgttgctttc cgtcgcgctc aaaaacaagc catccaacgc
    40801 tcaatgcgtg ctggcgctca agggatcaaa actcaagtat ctggtcgtct aaacggagca
    40861 gatatcgctc gttcagaagg atactcagaa ggaactgttc ctcttcatac tttacgtgcg
    40921 gacattgatt acgcatggga agaagcagat acaacatacg gaaaactagg agttaaagtg
    40981 tggatctatc gtggagaaat tcttccaact aaaaaaaaca ctgagaaagg agggaaataa
    41041 tcatgttagt acctaaacgt gtaaaacacc gtcgtgaatt ccgcggaaaa atgcgcgggg
    41101 aagctaaagg cggaaaagaa gtagcattcg gtgaatacgg tttgcaagct gttgattcac
    41161 attggatcac aaaccgccaa atcgaagctg ctcgtatcgc aatgactcgt tacatgaaac
    41221 gtggtgggaa agtatggatt aaaattttcc ctcacaaatc ttatactgcc aaagcaattg
    41281 gggtacgtat gggttctggt aaaggggcac ctgaaggatg ggttgcacca gtaaaacgtg
    41341 gtaaaatcat gtttgaaatc gcaggcgttc ctgaagaagt agctcgtgaa gcgttacgtc
    41401 tagcttctca caaattacca atgaaaacta agatcgtaaa acgtgaggaa atgggtggtg
    41461 aatcgaatga aggttaaaga aatcagagaa ttaaccactg ccgaaatgct tgatcaagaa
    41521 aaacaattaa aagaagaatt gtttaatctt agattccaat tagcaacagg tcaattagaa
    41581 aacactgcac gtattaaaga agtacgtaaa tcgattgcac gcatcaaaac tgtgttgcgt
    41641 gaacaagcta agtaattatg gaaggaggcc attaagcgta tgactgaagg aagaaatcaa
    41701 cgcaaagttt atcaaggtcg cgtggtttca gacaaaatgg ataaaacaat taccgttgct
    41761 gtcgaaacaa agaaaaacca ccctatctat ggcaaacgca tgaactattc taagaaatac
    41821 aaagtgcatg atgaaaacaa cacagcaaaa gttggcgaca tcgtgagaat tatggaaact
    41881 cgtccattat cagctacaaa acgtttccgt ttgttggaag tagtcgaaga agcagttatt
    41941 atctaataac acgagatttt cctatataga ttgaatgcaa aatctgaaag gaggatacac
    42001 aacgtgatcc aagcagaaag tcgtttaaaa gtcgctgata actcaggcgc acgtgaaatt
    42061 ttgacgatca aagtccttgg tggatctggt cgtaaaactg ctaacatcgg tgatgtgatc
    42121 gttgctacgg ttaaacaagc aacgccaggt ggggttgtca aaaaaggtga cgtcgttaaa
    42181 gccgttatcg ttcgaacaaa atcaggagct cgtcgtacag acggttcata tattaaattt
    42241 gatgaaaatg ctgcagtgat tattcgtgat gataaaagcc ctcgtggaac acgtatcttt
    42301 gggccagttg cacgtgaact acgtgaaaac aacttcatga agatcgtttc actagcacca
    42361 gaagttttat aatcttgaga cacttatcaa aggaggtgcg aaacagttat gtttgttaaa
    42421 aaaggcgata aagttaaagt tatcactgga aaagacaaaa acaaagaagg cgttgtacta
    42481 gcagcgttcc cgaaaaaaga taaagttatt gtcgaaggtg taaatgtcgt taaaaaacac
    42541 caaaaacctt ctcaagctgc tcctcaaggt ggaattgttg agatggaagc gccaattcat
    42601 gtttctaacg tgatggtggt tgactctact ggtgtagctg gccgagttgg ctacaaagaa
    42661 gtagacggca aaaaagtccg tgtttctaaa aaaaccggtg aagttttaga taaataaaag
    42721 tttaaggaag gagggtctta ctgaatgaac cgcctaaaag aaaaatatct taaagaagta
    42781 actccatcat tgatggaaaa atttaactat agctctgtta tgcaaacacc taaagttgaa
    42841 aaaatcgtca tcaacatggg tgttggtgat gcggtatcaa acgctaaaaa cttagataaa
    42901 gcagttgaag aattaacttt gatcactggt caaaaaccaa tgattacaaa agctaaaaaa
    42961 tcaatcgctg gattccgttt acgtgaagga atgccaattg gtgcaaaagt tacattgcgc
    43021 ggagaaagaa tgtacgaatt cttagacaaa ttggtttctg tttcactacc acgtgtacgt
    43081 gacttccatg gtgtaagtaa aaaagccttt gacggtcgtg gaaactatac tttaggtatt
    43141 aaagaacaat tgatcttccc agaagtagat tatgatttag tagataaagt acgtggtatg
    43201 gacatcgtta ttgtaacaac agcaaactct gatgaagaat ctcgtgagtt gttgacacaa
    43261 ttaggcatgc cattccaaaa ataattaaag gaggcgaact acgtggctaa aaaatcaatg
    43321 attgctaaaa acaaacgtcc tgcaaaattt tcaacacaag cttatactcg ttgcgaacgt
    43381 tgcggacgtc cacattcagt ttatcgtaag ttccatcttt gccgtatttg cttccgcgaa
    43441 cttgcctata aaggtcaaat tcccggcgtg aagaaagcta gctggtaaaa agtaaattcg
    43501 cataagaagg aggtaaaaga tcaatggtca tgacagatcc aattgcagat tttctaactc
    43561 gtatccgtaa cgcaaacatg gttaaacatg agtctttaga agtacctgca tcaaaaatta
    43621 aacgtgacat cgctgaaatc ttgaaacgtg aaggtttcgt tcgtgatgtt gaatacatcg
    43681 aagatgacaa acaaggcgtg atccgtgttt tcctaaaatt tggtaaaaac ggcgaacgtg
    43741 ttatcacaaa cttaaaacgt atctctaaac caggtttgcg tgcttatgtc aaagctgacg
    43801 aagtaccaaa agtattgaac ggtttaggta tcgcaatcat ttcgacttct gaaggtgttg
    43861 tcactgataa agaagctcgt gaaaaaaata tcggcggcga agttatcgct tacgtatggt
    43921 aatcaagtag aatctacaag gaggtgtctt taagtgagcc gtatcggaaa taaagtcgtt
    43981 gttatccctg aaggagtaac agttactcaa gacggaaaca acgttacagt taaaggacca
    44041 aaaggtgaat tgactcgtac tttttcagca gatatcaaaa tgaatatcga aggaaacgaa
    44101 gtaactttca ctcgtccaaa cgacagtaaa gaaatgaaaa caattcatgg aacaactcgt
    44161 gcgaacttca acaacatggt tgtaggtgtc agcgaaggtt tccaaaaagc gttagaacta
    44221 atcggggttg ggtaccgtgc acaattacaa ggatcaaaac ttgtattgaa cgttggttac
    44281 tcacatccag tagaaatcac accaccagct ggagtaactg ttgaagtacc agctaacaca
    44341 caagtaattg ttaaaggtgc gaacaaagaa gaagttggcg aattagctgc aaacattcgt
    44401 ggaactcgtc ctccagaacc ttataaaggt aaaggtattc gttatgttgg cgaatatgta
    44461 cgccgtaaag aaggtaaaac tggtaaataa tcgtttagaa aaattgcata gaagccagca
    44521 gcaggaaaaa ggcatttggc tgaagtttac cttcaaaaaa atacattttt acttgtgccg
    44581 cagcttctat ttttcgaaac gtatttactg cttgaaatag tagtacaata aaaataaaga
    44641 ggtgacaatt gtgattacaa aaccagataa gaacaaaaca cgtcaaaaga gacatcgtcg
    44701 tgtacgtaac aatatctctg gtactgctga gcgcccacgc ttgaacgttt ttcgttctaa
    44761 caaaaacatc tacgctcaaa ttattgatga cgtagcgggt gtaacgctag caagtgcctc
    44821 taccttggat aaagaaattt caggtggaac aaaaacagaa gcagcacaag ctgtcggtaa
    44881 actagttgct gaacgtgcag ctgaaaaagg cattaaagta gtagtctttg accgtggtgg
    44941 atacctttac catggccgtg tgcaagcttt agctgaagct gctcgcgaaa atggactaga
    45001 attttagaag aaggaggaat accattcatg gtttatatcg atccaaaaca tttggaatta
    45061 gaagaccgcg ttgttgcaat taaccgtgta acaaaagttg ttaaaggtgg acgtcgtcta
    45121 cgttttgctg ctttagttgt tgtcggtgac aaaaacggac acgtagggtt tggtacaggt
    45181 aaagctcaag aagttcctga agcaatccgt aaagcaattg aagatgcgaa gaaaaactta
    45241 gttgaagtac ctatggttgg ttcaacaatc ccacacgaag taatcggtgc ttttagtggt
    45301 ggtagaatcc taatgaaacc tgctgtagaa ggttctgggg ttgctgctgg tggaccagtt
    45361 cgtgccgtat tagaattagc tggggtagca gatatcactt ctaaatcttt aggctcaaat
    45421 acaccaatca acgttgttcg cgcaacagtt gaaggtttga aacaattgaa acgtgcagaa
    45481 gaagttgcag aacttcgcgg caaatcagtt gaagaattaa tcggataagg aggacaacaa
    45541 taatggctga attaaaagtt actttaaaac gcagtattat cggacgtcct caaaaccaac
    45601 gcgatacagt taaagcgtta ggtcttacaa agattaacag ttcagtggtt aagccttcta
    45661 atgaagcaat taaaggcatg atcaatactg tttctcacct agtggacgtt gaagaaattt
    45721 aatttagaaa aatcataact agaacattaa ggaggtgcca aacctatgaa acttcatgaa
    45781 ttaaaacctg ctgaaggttc acgccaagta cgtaaccgtg ttggtcgtgg tacttcatct
    45841 ggtaacggaa aaactgccgg acgcggacaa aaaggacaaa aagctcgttc aggcggcggt
    45901 gtgcgtttag gatttgaagg gggacaaaca ccattgttcc gtcgtttacc aaaacgagga
    45961 ttcacaaacg tgaaccgtaa agactatgca gtcattaatc ttgatgtatt gaaccgcttt
    46021 gaagacggag ctgaagtaac tcctgttgct ttagttgaag ctggaatcgt gaaaaacgaa
    46081 aaagctggaa tcaaagtatt agctaatggg gaactaaaca agaaattaac tgtgaaagca
    46141 gcaaaattct ctaaagcagc tcaagaagca attgaagcgg ctggtggatc aatcgaggtg
    46201 atctaatgtt tagactatta aaagatgctt ttaaagtaaa agacattaga tcgaaaatcc
    46261 tatttactgt atttgtgttg tttgtatttc gcctaggtgc acatattact gtaccaggcg
    46321 taaatgctgc ttcattgcaa aacctttctt ctttgccatt tttcaacatg ttgaacttgg
    46381 taagtggtag tgcgatgcaa aacttttcca ttttctcaat gggggtttca ccatacatta
    46441 ctgcttctat cgtcattcag cttcttcaaa tggacatcgt gcctcgattt gttgaatggt
    46501 cgaaacaagg ggaagttggt cgtaaaaaac tgaatcaagc gactcgatat ctgacattag
    46561 tattggcatt tatccaatct gtcggtatca ctgctggatt caatcaatac gctcaattag
    46621 gtttcgtgaa taatcctaac gtagcaactt acgtaacgat cggtttgatt ctaacagccg
    46681 gtacgatgtt tgttacttgg ctaggagaac agatcacaga aaaaggtatc ggaaatggcg
    46741 tatcaatgat catttttgca ggtatcattt ctagattgcc tgaaagtatc agagaaattt
    46801 atgaagatta ctttataaat attgatcctt cagacatatg gaaatctgct atttttgtgg
    46861 ccattttact agtagcggtc ttgttgatcg tgacattcgt aacatttttc caacaagctg
    46921 aacgcaagat cccgatccaa tatacgaaac gcgtagctgg cgcgcctaca agcagctatc
    46981 tgccgttgaa agtaaatgca gctggagtta ttccagttat ctttgctagt tcgtttattg
    47041 ctacgccaaa tgctgtttta caagcattga gcggtagatt cggttctgaa aactggtaca
    47101 aagtgatgat ggagatcttt aactataata cagcacctgg tgctacgatt tatacgattc
    47161 tgatcgttgc cttcactttc ttctacgcct ttgttcaagt aaaccctgag aaattagcgg
    47221 aaaacttgca aaagcaagga agttatatac caagcgtacg ccctggtaag gggactgaag
    47281 agtatgtctc aagattactg atgcgtttaa gtacagttgg tgcagtcttc cttggtttag
    47341 tggcattgct tccaatcatt gctcaaatgg tttgggatct tccacaatca atcggattag
    47401 gcggtacaag cttactgatc gttatcggtg tagctttaga aactgcgaaa cagttagaag
    47461 gcttaatgct gaaacgtaaa tatgttggat tcattgagta agaaagcgtg ttgaggtttc
    47521 ccctcaacac acgttctcac acatttatta ggagggaaaa caatgaacct cattttaatg
    47581 gggctgccag gcgcaggtaa aggaacacaa gctgaaaaaa tcattgatgc ttatggtatt
    47641 ccgcacatct caacaggaga tatgttccgc gcagcgatgc aaaacgaaac agctcttggc
    47701 ctggaagcaa agtcttatat ggataaaggt gcattagttc ctgatgaagt aacaaatgga
    47761 atcgtgaaag agcgactagc tgaaccagat acagagaaag gcttcttatt agatggcttt
    47821 ccacgtacgc tagatcaagc aaaagctctg gacgcaatgc taaaagattt gaataaaaaa
    47881 attgatgctg tcatcgatat ccatgttggt gaagaaatct tggtcgaacg tctagccggc
    47941 cgatttatct gcagcaattg tggagcaact taccataaaa ttttcaaccc tacaaaagtt
    48001 gaagacacat gtgatcgttg cggtgggcat gaattctatc aaagagaaga tgataaacct
    48061 gagacggtta aaaatcgtct ggctatcaac atcaaaaata gtgaaccaat cttggcttat
    48121 tataaagaac aaggtttgct aaacactatt gatggtgctc gtgaaatcga tgctgtattt
    48181 gcagatgtac aaaaaatcat tgaaaaatag cacgattgcc cgtagccgac ttgcattatg
    48241 cagttactct tgtcaagttc aataaattat gatagaatat gtcagtccga cttctagaga
    48301 atcctctaga tgatccattt tgagaatttt gaggtgggaa ctgcagtttc cgccatttta
    48361 tcgtgtgaaa atgcgaaaca agtacaagga ggtactgtgc gtggcaaaag aagatatgat
    48421 tgaagtcgaa ggtacagtcg tcgaaacttt gccgaatgca atgtttaaag ttgaactaga
    48481 aaacggacac caagttcttg ctactgtttc aggtaaaatt cgtatgcatt acatccgtat
    48541 cttacctggt gacaaagtaa cggttgaatt gtctccatac gatcttaacc gtggccgtat
    48601 tacttatcgc tttaaataat tgtactccgt aaaactacag gaggtattat catgaaagta
    48661 agaccatcag taaaaccaat gtgcgaacat tgtaaagtaa ttcgtcgtaa aggacgcgtt
    48721 atggtgattt gcccagcaaa tccaaaacat aaacaacgtc aaggataatt ggaggtgcta
    48781 taaatatggc tcgtattgca ggagtagata ttcctcgtga taaacgtgta gtagtttcac
    48841 ttacttatat ctatggtatt ggtaacacta ctgctaaaaa gatcttagct gatgctggtg
    48901 tatctgaaga tgttcgcgtt cgtgaattaa cgaatgaaca aacagatgct atccgtgcag
    48961 aagtagataa attaaaagtt gaaggggatc ttcgtcgtga agtgaacttg aacatcaaac
    49021 gtttgatgga aatcggttct taccgtggaa tccgtcatcg ccgtggattg cctgttcgtg
    49081 gacaaaacac gaaaaacaat gcacgtactc gtaaaggccc agctcgcgct atcgctggca
    49141 agaaaaaata attcttaagt gaaggaggtt aaaacttcat ggcagcaaaa aaagtgaatc
    49201 gtaaacgccg tgtgaagaag aatatagaag caggtattgc acatatccat tctacattca
    49261 ataacactat catcatgatc actgatactc acggaaatgc attagcgtgg tcatcagctg
    49321 gttcattagg tttcaaagga agcaaaaaat caactccttt tgccgctcaa atggcagcag
    49381 aaactgctac aaaagcagca atggaacacg gattaaagac tgttgaagta acagttaaag
    49441 gacctggttc tggacgtgaa gcagcaattc gttcattaca agctacaggt ttagaagtga
    49501 ctgcaattcg tgacgtgact ccagttcctc ataatggtgc ccgccctcca aaacgccgtc
    49561 gtgtttaatg agtgccgctc attttgagtt ttatcagtaa cgtcattgaa caagacactt
    49621 ttcgttttga aaggggtaaa gataagaatg attgagttcg aaaaaccaag aatcgcaaaa
    49681 attgatgaag aaaaagatta tggcaagttc atcgttgaac ctcttgaaag agggtatggg
    49741 actactttag ggaattccct acgtcgtatt ttattatctt ctttgccagg tgctgccatc
    49801 actaatattc aaatcgatgg tgtactgcat gaattctcta ccatcaaagg tgttagagaa
    49861 gacgtcactc aaatcatctt gaacattaaa ggcttagcgc tgaaaatgta tggccaagaa
    49921 gaaaaaaccc ttgaaatcga tattaccgga cctgctacag taactgctgg cgatattatc
    49981 gttgatagtg atgtagaaat tcttaacaaa gatatgtaca tctgtagtgt ctctgaagga
    50041 gctacgttcc atgctcgctt aacagtgaaa cctggacgtg gttatgttca agcagatgaa
    50101 aataaaaaag aagatatgcc aattggcgtt cttccagtcg attcaatcta cacacctgtt
    50161 cgtcgtgtga actatcaagt tgagaacaca cgtgttggac accgtgaaga ttttgataaa
    50221 ttgacgatgg agatttggac agatggttca attgaaccgt tggaagcaat gagtttgtct
    50281 gcgaaaatca tgactgaaca tttggatatt tttgtgaact taactgatga agcaaaaaat
    50341 gctgaaatca tggtcgaaaa agaagaaaca caaaaagaaa aaatgttgga aatgacgatc
    50401 gaagagttag acttgtctgt tcgctcttac aattgtttga aacgtgcagg aatcaatact
    50461 gtacaagaac ttacaaataa atctgagcct gaaatgatca aagtccgtaa cttaggacgt
    50521 aaatcattag aagaagttaa agcaaaattg cacgacttag gtttaggttt acgtaaagaa
    50581 gattaaaacc ttttacgaag gagggaaacc aacgtgagtt atcgtaaact aggacgcaca
    50641 tctagccaac gtaaagcgat gttgcgtgat ttaacaactg atttattaat caacgaacgt
    50701 attgtgacta ctgaagctcg tgcgaaagaa gttcgctcaa ctgcagaaaa aatgatcact
    50761 ttaggtaaac gcggagattt gcatgcacgt cgtcaagctg cggctttcgt acgtaatgaa
    50821 gttgccagcg tgcgtgaaga aaatgaggac atcgtcattg aatcagcttt acaaaaactg
    50881 ttcaacgata ttgcacctcg ttacgcagaa cgtcaaggcg gatacacacg cattttgaag
    50941 acagaaccaa gacgcggtga tggtgcacca atggttgtct tagaacttgt ctaaaatcac
    51001 actaataaca tcactttata gatgattctt ttagagtgtt atgatgttgg aagatatttt
    51061 tccgagtcta gctcagcgct gctcctcaag cgaatatcgc ttggggagta agcactcatc
    51121 ataaagtgtt tttttatacc ttcaattacc tgtcatgaca gatatgtttg aaggcataaa
    51181 aaaacatttt aagcgagaaa taattgaaaa ttttgtcttt ttttgatagt gttagtagga
    51241 actttaggat gaagaaggag taatcatgga gccaattatt gaattagaaa agataaatta
    51301 taaataccag ccagatgatc ttcgccccgc attgaaagat gtctccttta caattgataa
    51361 aggtgagtgg atcgctatta ttggacacaa tggttcagga aaatcgacac ttgccaaaac
    51421 gatcaatgga ttgcttctgc cagaatcagg gatcgtgaaa gtgggaaatc aaatattgga
    51481 tgaagaaaat atttggacga tccgacagat ggttggaatg gtttttcaaa atccagacaa
    51541 tcagtttgtt ggttcgacag tagaagatga cgttgctttt ggtcttgaaa atcaagggat
    51601 tcccagagaa gaaatgctag tgcgtgtgaa agatgcactt gagaaagtta gaatggctga
    51661 gtttgcttca cgcgagcctg ctcgtttatc aggagggcaa aaacaacgtg ttgccattgc
    51721 cggcgtggtt gccttacgac ctgatatcat tattttagat gaagcaacca gtatgctaga
    51781 tccagaaggt cgtgaagaag tgatctctac gatcaagaag atcaaagaag aaagtcaatt
    51841 gaccgttata tctatcactc atgatattga tgaagcagct aatgccaatc ggatactagt
    51901 gatgagacaa ggcgaacttg tccgtgaagg tacaccaaaa gaaatttttt ctgctggtcc
    51961 tgaactgatc gacttaggtc tagatctacc atttcctgaa aaactgaaga gcgcactgaa
    52021 agaacgtggg gttgatgtgc ccagtgaata tatgacagaa gaaaggatgg tggattggtt
    52081 atggacatcc gttttgaaca agtagacttc acttatcagc cgaatacacc atttgaacaa
    52141 agagccttat ttgacatcaa tatgacgatc aaagaaaaca gttatactgc attagtaggg
    52201 cataccggaa gcgggaaatc aaccttactt cagcatttga atgcgcttgt taagccaaca
    52261 agcggtacag tccatattgg agaacgagat attcagccgg atacggataa taagaattta
    52321 aagcccatcc gaaaaaaagt tggcatcgtc tttcagtttc cagaagcaca gctattcgaa
    52381 gaaacggtag caaaagacat tgctttcggt cctaaaaact ttggtgtcag cgaagaagaa
    52441 gcactagtcc tagcaaaaga aacattagaa caagttgggc tggatgaaag ctatttggaa
    52501 cgttcgccat ttgaactttc aggagggcaa atgcgtagag tagcaatcgc tggtgtgctt
    52561 gccatgaggc cggaagtact tgtattggat gaaccaacgg caggacttga tccccagggc
    52621 agaaaagaaa tgatggagat gttttggcgt cttcataagg aacagcagat cactattgta
    52681 ttagttacgc atttaatgga tgatgtagcc aattttgcag attatgtcta tgtgttggaa
    52741 aagggacgaa tcgttaatag cggggagccg caagaggtct ttcaaaacat tgaatggcta
    52801 aaagaaaaac aactaggcgt acctactgcc accgagtttg cagaagaatt aatggctaaa
    52861 gggatgaact ttgctacctt gcctttgaca gctgaagaat tagcagacgc tatcgtccat
    52921 tatgctggag gaaatgccca tgatgaataa attgatattt ggtcgttata tgcctggaga
    52981 ttccttgatc catcgattgg atccccgagc taaattatta gccagttttt attttatcgg
    53041 aatcattttt ttagcaaata attgggaaac gtaccttttg ttggcatttt tcacattact
    53101 ttctgtcttc ttgtcaaaag tagatttgaa attttttatc cgaggcattc gcccattgat
    53161 ctggctaatc ctcttcactg tagctttgca ggtcttgttt acaagtggcg gagaaactta
    53221 ttggagttgg ggtgttttcc atatcactga atttggtgtg atgaacggcc tctttatttt
    53281 ctgtcgattc gtgttgatca tttttatgtc gactctactc acgcttacta caccgccttt
    53341 ggaattatcg gatgcgattg agtacttgct tcgtccattg aaagccgtac ggttcccagt
    53401 ccacgaaatt tcattgatgc tgtcgattgc tcttcgtttt gtgcctacat tgatggacga
    53461 aacagaaaag atcatgaacg ctcagcgcgc tcgcggtgtt gattttggtg aaggaaacct
    53521 agttcagaaa atgaaagcag tcattccttt gctgattcct ttatttgtca gcagcttcaa
    53581 ccgtgcggaa gatcttgcga ctgctatgga agcaagaggc tacaaaggtg gggaaggaag
    53641 aacgaaatat cgtgtattgc attggcataa tagagacacg atggttatca ttgcttttgt
    53701 attattaact gtgctactga tctttttaag aggataaacg ttcagtgggc cgctatctat
    53761 tcttttcttg ctgatctgtt tgcaagaaag gatgcaagca gtgtatgaca agaaatatta
    53821 gggtctgtgg cagactaagg tcacaggcct ctttctttta tctgaaaaga aagaacgaaa
    53881 aa
//
LOCUS       DAYYBH010000013        50197 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000013 DAYYBH010000000
VERSION     DAYYBH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.013"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1736
                     /locus_tag="ACHAUG_000892"
     CDS             75..1736
                     /locus_tag="ACHAUG_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8480004.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1806..2153)
                     /locus_tag="ACHAUG_000893"
     CDS             complement(1806..2153)
                     /locus_tag="ACHAUG_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8480005.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2237..3178
                     /gene="opp3b"
                     /locus_tag="ACHAUG_000894"
     CDS             2237..3178
                     /gene="opp3b"
                     /locus_tag="ACHAUG_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8480006.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3179..4222
                     /locus_tag="ACHAUG_000895"
     CDS             3179..4222
                     /locus_tag="ACHAUG_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480007.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4228..5298
                     /locus_tag="ACHAUG_000896"
     CDS             4228..5298
                     /locus_tag="ACHAUG_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480008.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5288..6241
                     /locus_tag="ACHAUG_000897"
     CDS             5288..6241
                     /locus_tag="ACHAUG_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480009.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6253..7263
                     /locus_tag="ACHAUG_000898"
     CDS             6253..7263
                     /locus_tag="ACHAUG_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8480010.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7548..8369)
                     /locus_tag="ACHAUG_000899"
     CDS             complement(7548..8369)
                     /locus_tag="ACHAUG_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8480011.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8466..8744)
                     /locus_tag="ACHAUG_000900"
     CDS             complement(8466..8744)
                     /locus_tag="ACHAUG_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480012.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9012..11021
                     /gene="metG"
                     /locus_tag="ACHAUG_000901"
     CDS             9012..11021
                     /gene="metG"
                     /locus_tag="ACHAUG_000901"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8480013.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11500..12087)
                     /locus_tag="ACHAUG_000902"
     CDS             complement(11500..12087)
                     /locus_tag="ACHAUG_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480014.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12262..13584
                     /locus_tag="ACHAUG_000903"
     CDS             12262..13584
                     /locus_tag="ACHAUG_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8480015.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13739..14515
                     /locus_tag="ACHAUG_000904"
     CDS             13739..14515
                     /locus_tag="ACHAUG_000904"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8480016.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14508..15077
                     /gene="rnmV"
                     /locus_tag="ACHAUG_000905"
     CDS             14508..15077
                     /gene="rnmV"
                     /locus_tag="ACHAUG_000905"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8480017.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15070..15954
                     /gene="rsmA"
                     /locus_tag="ACHAUG_000906"
     CDS             15070..15954
                     /gene="rsmA"
                     /locus_tag="ACHAUG_000906"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8480018.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16405..17034
                     /locus_tag="ACHAUG_000907"
     CDS             16405..17034
                     /locus_tag="ACHAUG_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480019.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17158..18267)
                     /locus_tag="ACHAUG_000908"
     CDS             complement(17158..18267)
                     /locus_tag="ACHAUG_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480020.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18476..18898
                     /gene="mgsA"
                     /locus_tag="ACHAUG_000909"
     CDS             18476..18898
                     /gene="mgsA"
                     /locus_tag="ACHAUG_000909"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8480021.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19085..19183
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19221..19757
                     /locus_tag="ACHAUG_000910"
     CDS             19221..19757
                     /locus_tag="ACHAUG_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8480022.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19797..21560)
                     /locus_tag="ACHAUG_000911"
     CDS             complement(19797..21560)
                     /locus_tag="ACHAUG_000911"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480023.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21563..23278)
                     /locus_tag="ACHAUG_000912"
     CDS             complement(21563..23278)
                     /locus_tag="ACHAUG_000912"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480024.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24057..24281)
                     /locus_tag="ACHAUG_000913"
     CDS             complement(24057..24281)
                     /locus_tag="ACHAUG_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480025.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24472..27249
                     /locus_tag="ACHAUG_000914"
     CDS             24472..27249
                     /locus_tag="ACHAUG_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8480026.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27330..27749
                     /locus_tag="ACHAUG_000915"
     CDS             27330..27749
                     /locus_tag="ACHAUG_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8480027.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27761..28231
                     /locus_tag="ACHAUG_000916"
     CDS             27761..28231
                     /locus_tag="ACHAUG_000916"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8480028.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28248..29027
                     /locus_tag="ACHAUG_000917"
     CDS             28248..29027
                     /locus_tag="ACHAUG_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8480029.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29014..29835
                     /locus_tag="ACHAUG_000918"
     CDS             29014..29835
                     /locus_tag="ACHAUG_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8480030.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            29852..30901
                     /locus_tag="ACHAUG_000919"
     CDS             29852..30901
                     /locus_tag="ACHAUG_000919"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8480031.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            30914..31918
                     /locus_tag="ACHAUG_000920"
     CDS             30914..31918
                     /locus_tag="ACHAUG_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8480032.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32551..33570
                     /locus_tag="ACHAUG_000921"
     CDS             32551..33570
                     /locus_tag="ACHAUG_000921"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8480033.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33613..34494
                     /gene="galU"
                     /locus_tag="ACHAUG_000922"
     CDS             33613..34494
                     /gene="galU"
                     /locus_tag="ACHAUG_000922"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8480034.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            34794..35171
                     /locus_tag="ACHAUG_000923"
     CDS             34794..35171
                     /locus_tag="ACHAUG_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480035.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            35777..36391
                     /locus_tag="ACHAUG_000924"
     CDS             35777..36391
                     /locus_tag="ACHAUG_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8480036.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            37164..38903
                     /gene="yfcC"
                     /locus_tag="ACHAUG_000925"
     CDS             37164..38903
                     /gene="yfcC"
                     /locus_tag="ACHAUG_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8480037.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            39060..40244
                     /gene="iadA"
                     /locus_tag="ACHAUG_000926"
     CDS             39060..40244
                     /gene="iadA"
                     /locus_tag="ACHAUG_000926"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8480038.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            40355..40558
                     /locus_tag="ACHAUG_000927"
     CDS             40355..40558
                     /locus_tag="ACHAUG_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8480039.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(40659..41321)
                     /locus_tag="ACHAUG_000928"
     CDS             complement(40659..41321)
                     /locus_tag="ACHAUG_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480040.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(41334..42836)
                     /locus_tag="ACHAUG_000929"
     CDS             complement(41334..42836)
                     /locus_tag="ACHAUG_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480041.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            43384..45063
                     /locus_tag="ACHAUG_000930"
     CDS             43384..45063
                     /locus_tag="ACHAUG_000930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8480042.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            45190..45669
                     /locus_tag="ACHAUG_000931"
     CDS             45190..45669
                     /locus_tag="ACHAUG_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8480043.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            45888..46568
                     /locus_tag="ACHAUG_000932"
     CDS             45888..46568
                     /locus_tag="ACHAUG_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8480044.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            46576..47907
                     /locus_tag="ACHAUG_000933"
     CDS             46576..47907
                     /locus_tag="ACHAUG_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8480045.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            47922..48884
                     /locus_tag="ACHAUG_000934"
     CDS             47922..48884
                     /locus_tag="ACHAUG_000934"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8480046.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            48995..49333
                     /locus_tag="ACHAUG_000935"
     CDS             48995..49333
                     /locus_tag="ACHAUG_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8480047.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            49412..50119
                     /locus_tag="ACHAUG_000936"
     CDS             49412..50119
                     /locus_tag="ACHAUG_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8480048.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT        16342 a         8797 c        10454 g        14604 t
ORIGIN      
        1 attattcatt aaactcttaa acaacttcag aaaatattaa ttattctgaa aattccaata
       61 aaggggtacg taaaatgaaa aagaaattta catttggttt ggttgcggta tgcggccttg
      121 tacttgctgg atgttatggc ggcagttcag ccacaagcag tagttcagca aaaagcggtt
      181 cagcagatgg aggaggcgta tttaacttag tcgtgcctca agaaatgcct acagctgact
      241 tatcagttgc aacagataca atcagtttta cagcattgaa taacgtttat gaaggaattt
      301 accgtcttga tgaagacagc aaaccacagc cggcaggagc tagcgagttg gcagaagtca
      361 gtgaagacgg attaacttac aaaatcaaat tacgagaaga cgcaaaatgg tcaaatggtg
      421 atcctgttac agcagcggat tacgtttatg gctggcaacg aacagtagac ccagctacag
      481 cttctgaata tgcttacctg tatgcgccag ttgaaaatgc agaagacatt acagctggaa
      541 agaaagataa atcagaacta gggatcaaag cagttggtga ctatgaatta gaaatcaaat
      601 tgacgaaaca aacaccatat ttccaatatc tgcttgcttt cccatcattt ttcccacaaa
      661 aacaatccgt agtggaagaa aatggggatg cctatgcatc tgcaagtgat aaagctgttt
      721 ataatggacc attcacatta gaagattttg atggaccagg aacagataca gaatggactt
      781 ataagaaaaa tgatcattac tgggacaaag atacagtaaa actttctgaa atcaaagtca
      841 gtgtagtaaa agaatcttct actgcattga acttattcaa agatggccaa gcagatgacg
      901 tgatcttgtc tggtgagtta gcacagcaaa acgcaaacga tccagcttat acttctgtta
      961 aagaagcgcg gacaagctat atcgaattca atcaacgtga aaagaattca ccattcaaca
     1021 atgtgaacct aagaaaagct atttcttatt ctatcgatcg tgaagcttta gtaaaacaag
     1081 tactaggtga cggatcagtt gtctctacag gattgatccc atcagatatg gtaaaaagtc
     1141 caacaacgaa cgaagacttt gcaaaagaag ctggaaaatt agtttcttat gacaaagata
     1201 aagcaaaaga atattgggaa aaagcgaaaa aagaattggg cgttgattct ttagaatttg
     1261 atttgatggc ttctgatgat gattcatcga aaaaagtaat cgaatatgtt caaaactcaa
     1321 tccaagaaaa cttggatggc gtaacagtca aaccaacacc tgttccattc tctgtacgtc
     1381 ttgaccgctc aacttcgggt gattttgacg cagtactagg cggatgggct gctgactatg
     1441 cagacccaag cagctttact gatttgtttg tcacaggtaa ttcatacaac cgtggtcgct
     1501 ggagcaatgc agaatatgac gaagcagtta aagcagcttc atcaacagac gctggcgatg
     1561 aacaagcccg ttgggatgat ttccaaaaag caatcaagat catctctgat gatatgggtg
     1621 tcgttccggt gtaccaaaaa gcagaaggtc acctagtgaa tgaaaaaatc aaaggtatcg
     1681 ttcaccatgc ggcaggcgct tcatgggatt acaaatggac atacgttgaa gaataatcgt
     1741 taataccgtg agcatgagaa aaaacgaggc tgcggcaaat gtcgcaacct cgttctcata
     1801 ttcacttatc ctaacaaaga aaaaagcatg aaaatagcag aaagagcaat tagtattccg
     1861 gcaatgatca gccaaaagct gtacataccg tagctggcag acgagagcga ggaaaattct
     1921 ggttttttct ttcggttcct tgttcgtgca agatggaccg atcgctgaaa atgatcgaag
     1981 aaacccgaag aaaagaccca taaaaaaccg ccgataatca gaaacggcaa tgaccataag
     2041 aatagatcat tagataattg aatgagtgta aaggaatggt tgatgagatt ttttattaaa
     2101 ataaccagaa tcagtagaaa agctactgcg taggggatag attttttttt catttgattg
     2161 ctcctttatt tttggttcat tatctaataa tgtagcgaaa catctgtcag aagtcaaaag
     2221 ctagggggaa acattagtga atagttatat caaatatgta ttaaaacgtg tctttttcat
     2281 gatcatcacg ctttggttaa ttgcaacaat cacctttttc ttgatgcaat tgctgccggg
     2341 cacgccatat acgaatcagg aacgtttgag tcctgaaaca atcgagatgc taaataaaca
     2401 agtcggtttg gataaaccag ttatcgtaca atatgggatt tatctttcta atttgcttca
     2461 aggagatttt ggaatttcct tccaatttaa aaaccagcca gtcgcacatt tgttagctgg
     2521 aagaatcgga ccatccttgc agttagggtt acaggcaatt atctttggaa cagtcctagg
     2581 gacgatttta ggaacgatct ctgccatgaa gcaaaataca tgggcagata catcttctac
     2641 actcgtagcg atattaggtc gttccattcc aaactttgta tttgctgttt tattacaata
     2701 tatctttgca atcaaattac aagttttgcc aatcgcaaaa tgggatggct ttgtgtacac
     2761 aatcttacca acaattgcgt tggccatgtc gccgcttgct gactctgctc ggtttatccg
     2821 tacggaaatg gtggaagtac tgcatagtga ttatgtggaa ttagcaaaag caaaaggatt
     2881 gagccgttgg gaaatcgcct ttaaacatgg tcttcgaaat agtttgattc cattgatgac
     2941 tttattagga ccgttagctg tagcattgat gaccggatca ctggtggtag aaaatatctt
     3001 tgctattcct ggtatcgggg aacaattcgt taagtcaatc acaacaaatg attatcctac
     3061 gatcatggct gtaacgatcc tttactcatt tatgctgatt tttgttattt tgatcgtgga
     3121 tctattgtac ggtctagttg atccaagaat tcgtgtttca gaagggagcc gaggataaat
     3181 ggaaatgata ccaaagcgtt atgaaacagt tgcagatatc cctgcggatg aatttcttcc
     3241 attacaaaaa aataccgaag aagaacgtga acagattact gcaccttctt tgaattttat
     3301 ccaagattca tggcgccgtt taaagaaaaa caaagctgcc gttatttcaa tggttttact
     3361 attggtcatc attttgatct ctgtgatcac aatctttgtt tctcctcatg atccaactgc
     3421 ccaaaacgtg gcttatatca atttgcctcc tcgtattccg ggaatcaata tcgatggatt
     3481 gaatggaaaa gcaatggttg gtggtgaact tgttgataaa tatgctcaag caaacgtccc
     3541 ttcaaacgta aatttctttt taggaacaga cggtttggga cgtgatgtat taagccgctt
     3601 atttatggga atgagaatct cgttgctgat cgcatttatt gctgcattac tagatgtaac
     3661 gattggtgta gcttacgggt tggtttctgg tttgttaggc ggacgtgtcg ataacgtgat
     3721 gcaacgtttt ttggaaatct tatcaggaat tccaaacctc gttgtcatga ttttgatgtt
     3781 ggtcgttttt gaaccaggaa ttttttcaat cgtggctgct atggccatca caaactggat
     3841 accaatggca cgtatcgttc gtgcacaaac aatgaaatta aaagatcaag aatatgtttt
     3901 agctggattg acattaggtg aatcaagaat gaaaattgca ttcaaacata ttttgccgaa
     3961 tatctctagt gtcattatta ttcaaatgat gtttagtatt ccatctgcta ttttcttcga
     4021 agccttttta agctttatcg gtttaggtct tacgcctccg tcagcttcat taggaacgat
     4081 gttgagcgaa gggtacaaga cattcctgta tctgccttat ctattatgga ttccggcagt
     4141 aactttatcg gttatcatga ttggctttaa cttacttgct gatggattgc gtgatgcctt
     4201 tgatcctaaa atgaaagagt gaagacgatg acgaaaattt tagaagtgaa agacttagaa
     4261 atctcttttg atacgtatgc tggaaaggta cgagcgattc gcggtgtcaa ttttcatttg
     4321 aataaaggag aaacattggc gatcgttggg gaatctggaa gtggaaaatc agtgacgacc
     4381 agaagcatca tgcgcttgct gtcaagcaac gccaatatag atgctggtca aatcctcttt
     4441 aaaggtaacg atatcgttgg aaaatcagaa agagagatgc aaaaaatccg tggtaaagaa
     4501 atagcgatga ttttccaaga tccaatgacc tctttagatc cgacgatgcc cattgggaag
     4561 caagtcgctg agtcccttag aaagcataat aaagtttcaa aaaaagaggg acaaaaagct
     4621 gcattagaat tgcttaaatt agttggaata cccgaagctg agaaacgtat caacagttat
     4681 ccgcatcagt tctcaggagg tcagcgtcaa cggatcgtga tcgcgattgc actggtatgt
     4741 tatccggaaa tactgattgc tgatgaacct acaacagcgc ttgatgtgac tattcaagca
     4801 cagattttgg agctgctgaa agaaattcag acaaaaatcg atacatcaat tatctttatc
     4861 actcatgacc ttggtgtagt agcgaatgtc gctgatcgtg tcgctgtcat gtatggagga
     4921 agaatcgttg aagttggaac atcggaagaa attttctaca acccgcaaca tccctatact
     4981 tggggactgc ttggttcaat gccgacatta gatagtgcga acgatcgatt gtatgctatt
     5041 cctggttctc caccggattt attgaacccg ccaaaaggag acgcattcta tccaagaaat
     5101 gaattcgcga tgaaaatcga tgcagaacaa gaaccaccat tttttgaact atcaaaaaca
     5161 cacaaggcag caacttggct acttgctccg caggcaccca aagtgacacc gccagaagaa
     5221 atccaacgtc gctgggctat ctttaaagag aaacaaaaac atacttacgg tcaaggagga
     5281 attgctgatg ccaaataaac tacttgaagt taaaggattg aaacagtatt tcaatgtagg
     5341 aaaaaagaat gaagtacatg ctgtaaatga catcagcttt cacatttatg aaggagaaac
     5401 attcggttta gttggagaat ctggaagcgg aaaatccact actggccgta cgatcattcg
     5461 cttgaatgaa cctactgatg gtgagatatt attcgacggt caagatataa cgaagatcaa
     5521 agataaaaaa ggtttgacga aattccggca tgatgttcag atgatctttc aagatccata
     5581 cgcttcgttg aatcctcgta tgaaagtcag agatatcatt gccgaaggaa ttgatgttaa
     5641 tggactagct aaatcatctg aagagcgtgc aaaaaaagtc gatgatctat tgagaactgt
     5701 tggattgaat ccaagccacg gtactcgtta tcctcatgaa ttttcaggtg ggcaacgcca
     5761 gcgtattggt attgcacgag cattggcagt aaaaccgcgt tttatcattt gtgatgaacc
     5821 aatttctgca ctagatgtgt cgatccaagc ccaagtggtc aatttgttgc aagatctcca
     5881 aaaagaacat caattgacct atctatttat tgcacatgat ttgtcgatgg ttaaacatat
     5941 tagtgaccga atcggtgtaa tgcataacgg cttgttatta gagatgggaa caagtgatga
     6001 aatctataat cacggtgttc atccttatac agaaagtttg ttatctgcta ttcctctacc
     6061 agatccagac catgagagaa aacgcagaag aatcaaatat caacctgaac cagatgacgg
     6121 acaagtacgt caacttcgtg aaatcgcacc agaacatttt gtctatgcaa ctgagcagga
     6181 agtcccatat tacgcaaaaa aactaaaacg acaaaaggaa tctcttctgg ttgctaacta
     6241 ggagggacat agatgctaaa agcttttaaa ttccgtatgt atccgacaga agaacaaaaa
     6301 cagcaattga ttcgtacttt tggatgtgct cgatttacct ataaccattt gctgaaaaag
     6361 cggcagaaaa gttggcaaca aacaggagta gccaattttt cgctcacacc cgctacatta
     6421 aaaaaagaat acccattctt aaaagaagta gacagtcttg ctttagcaaa tgcacagctt
     6481 aatttggatc gggcttttcg caattatttc aagggacgtg ccagcttccc aaagcttaaa
     6541 acgaaaaaaa gtatgtggca atcttatacg accaacaacc aaacccgtac gatctatctg
     6601 gaaaatggct acttaaagtt accaaaacaa aaagaactaa tcaagataaa tcagcatcgg
     6661 ccagttgaag gatcgattcg ctccgctacg atttctgctc gatataatga agaattttat
     6721 gtcgcactac tttgtgatgt ttcgccagta aaaaaagaat ctttagctaa atggatcggg
     6781 atcgcctatc atccgaagac attgatccaa acatctcgac ctctagaagt aactttacca
     6841 aaatttcacc agacagaaga aaagctgcaa catgcacagc ggaaattgaa tgtgaaagtc
     6901 cgctccgctc accatagaaa gatcaggtta gatcaggcaa gcaattatca gaaacaaaag
     6961 agaaaagtaa tggatcttta tctgaaacaa aagaaccagc gagaagatta tcttgaacaa
     7021 ttatctggga aattggtcaa acaatatgac tacttatttg tagaatcttt tccaaaagaa
     7081 gaggcacatg ctgatttttc tattcacgac tggcataaat taataacaaa attgcgttat
     7141 aagtcccaat ggtataataa aaaatttctt ttgatcaata ccgacggagc agaagaaagc
     7201 aattccgtaa gaaagagcca agtagtagaa aaaatgggca gacattcggt tatcaaggga
     7261 taaaaaatga atgaaactga taggagcgtt agggttagcc ttgttaataa aaagtcttaa
     7321 tgacttttgt ttctaggaat ttttcttttg aagaaaagct cactaattat gacaaaccaa
     7381 taaaacaata gcagaaaaag ttcgtacgag ctttttctgc tattgttaat tttttattca
     7441 gaaagtttac gaatacatca aaattctgaa acaaaaaacg taactcacaa agagttgttt
     7501 ttgcgttttt tcttgaatga ttaggtctga atcttttgct cacaacctta ttcatggtgc
     7561 cccggcttga gctgatagat ttttgaagga cggccgattc ctacaggacg ttcaccgatc
     7621 tcatcgaaat attggagcat agctttctta aaattggaat gatcgatcgt tttataatcg
     7681 atccctaaga atttagcaaa tacttttcga gcttccgtaa tcgtgaaatc tttacctaaa
     7741 acttgtagaa cttgcggctc gtgttccatt ttattgacta cacggttgaa tgctttcaag
     7801 atgatttgac tatggtcaaa agccaaagta tctttgccag aagactctcc agtgactaaa
     7861 tcaagcgaga tagccacttc tccgctagat aaatggatct tgttactatg acgttctagt
     7921 gtgaaccagc gtacttcctt ggcatcatct ccggcgatta gcggttcttc tccaataaag
     7981 gctaagtagc tgactgtgac gacccagcct cgcggatctc gattaggcgt actgaatgta
     8041 tgcagttgct caatattttc ttttgaaata accacaccag tttcttcctt tgtttctctt
     8101 aagacgcttt ctccagttga ctcatccttt tggacaaagc caccaggcaa tgcccatgaa
     8161 tttcgataag gatgcccttt tctttggatc aacaaaactt tcagctgatc ttcttctttg
     8221 ttatagcaca tcagtacgat atcgacagtc aaagagggct tgtcatattc tggacgttcc
     8281 tgtttttgat accagttaag aaattcttct tcacttgctt tatgttcata atataatttt
     8341 tcttcttctt tagaagcaaa acggttcatc tgaccacctc cattgacagt aaaagtactt
     8401 tttaccttat tgtagcagac tctgaaaaaa aaccaaggat ttacttgaaa aatctaggtt
     8461 atcatttaaa tatgaaaaat aaatggaagt atgagattga ataaaaagaa aagcagataa
     8521 aacggcaata ctttataccg ctcttcacga atggcaaaga agccacaaat aacgatatat
     8581 acgtcaatga taaaacgcaa aatccaagct gctgtcataa aactgttcaa atgcccatgg
     8641 aggttaaaat aaccatacat acgaatataa aaatagctga atattaaatg tgctaatgaa
     8701 agaaaagcaa tactaagtgt aaaaaaaaga tgtttcttct gcatgattgt tccagtccct
     8761 tactaagata gatagtcttt attataccac gtgtgagggg agaaataaaa agccgttttt
     8821 tagtagatat gaaacaaaac aaaaacccgc atttattcaa cctattcatc cttttaaagg
     8881 atagattgaa cgtagataga agtcgtttat ccagtctttt tcattttgaa aaaaagctaa
     8941 cagcatgctt tttttatggt atactagcaa ggaaacgaat tcgcatcatg cgataaagga
     9001 ggaagagccc cgtggcagaa aaggaaactt tttatatcac tacgccaatc tattatccaa
     9061 gcggcaaact tcatatcggc aactcgtata ctacgattgc atgtgacgca attgctcgtt
     9121 ataaacggtt gatgggcttt gatgtgtttt atctcactgg agtggatgaa catggtcaaa
     9181 aaatcgaaac aaaagcagaa gaactaggtg taaaaccgca agaatatgta gataaaatgg
     9241 cggctgatgt caagaaatta tggaaaacgc ttgatatcag ttatgataaa ttcattcgca
     9301 cgacagatga ttaccataaa gcagctgttc aaaaaatatt tgatcgttta ttagaacagg
     9361 gagatattta cttaggtgaa tatgaaggct ggtactctgt ttctgacgaa gaattcttta
     9421 cagaaacaca acttgcagaa gtgtacaaag atgacgaagg taacgtgatc ggtggtaaag
     9481 cgccaagtgg acacgaagta gaattggtaa aagaagaatc atacttcttc cgcatgagca
     9541 aatacgcaga ccgtctattg gaatactatg aagaacatcc cgaattcatc cagccagaat
     9601 ctcgtaaaaa cgaaatgatc aacaacttta tcaaaccagg attagaggat cttgcagttt
     9661 cacgaacaac ttttacttgg ggagtaccag tcaagagtga tccgaaacat gtggtgtatg
     9721 tatggatcga tgcattatcc aattatatta ctgcactagg atatggctcc gaagatgaaa
     9781 gtctattcga aaaatactgg cctgcagatg ttcagatggt cggtaaagaa atcgttcgct
     9841 ttcatacgat ctattggcca atcatgctga tggcccttga tttgcctcta cctaaaaaag
     9901 tatttgggca tggctggcta ttgatgaaag acggaaaaat gtctaaatca aaaggaaatg
     9961 tcgtttatcc tgaaatgctt gtggaacgtt atggtttaga tgcactccgt tattatttgc
    10021 ttcgtgctgt tccgtttggg tcagatggtg tctttacgcc agaagatttt gtttcccgtg
    10081 tcaattatga tctggcaaat gatctaggga acttattgaa tcgaacgatt gctatgatca
    10141 acaaatattg tgatggactc gttcctgact atgcctcact tgtcactcca tttgatagtg
    10201 aactgtctac gacggctgct aacgttgtcg gacgttacca tgatgcaatg gaaaaaatgg
    10261 aattcaatac agcattggct gaaatctggg tgctgatttc tcgtgcaaac aaatacattg
    10321 atgaaacaga accatgggta ttagcaaaag acgaagaaaa gaaagctgaa ttgaatagtg
    10381 tcatgatcca tttagctgaa tctttgcgta tcgcagcgat attacttcag ccaatcatga
    10441 cagaaacgcc agagaagatc ttcaatcaac taggattgga ctctgaaaca atgaatcttg
    10501 aaggattaca ctttggcgaa tttccttcag gaacaaaagt agttgcaaaa ggtacaccaa
    10561 tcttcccacg tttagatatg gaagaagaag ttgcttttat tcaagaaaaa atgtctgaag
    10621 gtactcaaac aaacgaagat acagtcaaat gggatccaga agaaacagaa cttgtttcaa
    10681 cgaaagaaaa acaaatcaaa tttgatgtat ttgaaaaagt agaactaaaa gtagcagaag
    10741 tcatcaactg tcaaaaagta gaaggggcag ataagttgct gcaattccga ctagatgctg
    10801 gagacagcca agaccgtcaa atcttgtcag gaatcgctga attttatcct gatccaagcg
    10861 aattgatcgg gaaaaaagtc gtcattgtag caaatctgaa accacgaaag atgcgtggac
    10921 aaatcagtca aggaatgatc ctttctgcag aagcaccaga tggctcttta caagtaatcg
    10981 aagcaccaaa atccatgcca aatggctcag aaatcgcata gaaattcaaa gggggtatga
    11041 cactagttgt gtcatacccc ctttttccga aatttgagaa gcaattttcg taaattcctt
    11101 cttattactt gaagccaatc atataataca gaaacagaga actatccaaa ataaatccta
    11161 gaaaaagaag tacgagcttc gctgattttt gttaatttag aataatacta ataggaatcg
    11221 taaaaacaga aaaaaagcat aatgataaag ggtcgggcag attttaattg attcatctgg
    11281 tactcttttt tattgtcctt acattcataa agtttgaatg caatgacttt attacatcta
    11341 tatttatatc taacagtagc gttgatgtgt taattataag ttctatcact ttatgttcaa
    11401 ttagtttgtg ctcttatatg tgtgattgaa aatatagata aaaaaacaat catcggcttt
    11461 gacttcgatg attgttttct ttattttgta aaaatcctat cacttgattt tatggtaacc
    11521 ttttattttt gtcttcaaac ggcttggtaa gagagcagta agcagtgcaa atgggatcga
    11581 aaagacatac cagaaaatcg tacgtccgat accataatgt tgatagagct ttgtcaacat
    11641 cacgtgtaac aagatcaacc ctaaaagcaa ggagccagct ataaaccaat gattatccca
    11701 atgcatgact tcactaagtg ccatcactcc aaaaggatag aaataaaaat agatcccgac
    11761 taaggtcatc cgccagttct ttgtaaatgt tccgatattc aataaaaaga aaatcgctga
    11821 aagcaaaata ccgataggcg gaaaaagccc aaccaaaaag gaatacaaca tcccccacaa
    11881 gatcatcaca ggacctttga tcaatgcaac gattgcgatg aacaaagtca gcaatattag
    11941 tttttgtgat aacgttgcaa cagtcattaa tccaaataaa attaatgtga accaatgcca
    12001 atgggactgc tgttgttctt tacgggaaaa atcgacccgg ttggataaat aatctgctaa
    12061 tttcggataa gaacgcttaa aatccataaa ctgttcctcc ttcgtggaaa aggtaggttt
    12121 agtatacgaa aaataaaaaa gaaatacatc cgtcttaaga gggagttttt cactgaaagt
    12181 ggagaaaaaa gaatggtaaa ttaaaatatt ctcagttata ctgaaaatta taccaaattt
    12241 caaagaagaa agaggatggg aatgaaaggc aaactaaaac gagaaatcaa tctgtttggc
    12301 gcattagcta ccgttatggg aaccgtgatc ggtgcaggcg tcttcttcaa aactgctgct
    12361 gttactgcta gtactcagtc tacaagtttg acattgctag cttggctttt gggtggtttc
    12421 ttaacgatct gtgctggatt gacagtggct gaattagcta cagcaatccc ggaaactggt
    12481 ggagctgtaa aatatattga agctgcgtat ggaaaattgc ccagcttttt attaggatgg
    12541 gcgcaaagct tgatttactt tcctgctaat attgctgcat tatcgattat ctttgcgaca
    12601 caattgacga acttattaca attatctacc gattatcttt tgttgattgc agtgatcacc
    12661 gctgtttctg tgacagggtt gaatttgtta gggacaaaag tcggtgcttc tgtacaatca
    12721 gtgacgttga ttgttaaatt aataccaatt gctgtgattg tcatatgggg attattgacc
    12781 ccaggacaag gaactgtcca gttatttcca atcgaagctg gaaaagatgt tacttttgtc
    12841 gaaggattga gcagtgcgtt gttagcaacg ttgtttgctt atgacggctg gttaggtgtc
    12901 ggtgcaatgg caggggaaat gaaacggccg gaaaaagatt tgcctaaagc aattattctt
    12961 ggtttgagtt ttgtaacagt cgtttatcta ttgatcaatt ttgttttttt gaaaactttg
    13021 ccgattgatc atcttgccgg taatttgaat gcagcttctg aagcatcaga cgtcattttt
    13081 ggaggaatcg gtggaaagct ggtcactatt gggatcttga tctcggtata cggtgcattg
    13141 aacggttata cactaactgg gatccgcgtg ccttatgcga tggctttaga agacgagcta
    13201 cctttcagta aacagctgac aaagctttca aagaagttta cggttcctta tgttcctgcg
    13261 gttttccagt tagcagtagc ttgtattatg atgagccttg gttctttcga ctttttaaca
    13321 gacatgttga tttttgtgat gtggctattc agtttactga tttttatcgg tgtctttatt
    13381 ttaagaaaaa aagcgccgga attgccacgt ccatataaag taccacttta tccaatcgtt
    13441 ccgattattg cgattctcgg tgctattttc attttaggta tgacgatgat gacgcaaacg
    13501 aaattagcat tgatcggtat aggtgttacc ttgataggta tcccagtcta ttatcaaaaa
    13561 aagaaaaaac gctcagaaga gtaaaatatt tcaacaacag acagaaaaag tacgaactag
    13621 gtcctctaga tcttcgtgct ttttctattt caataaaaca agttcttcta ttttgatttt
    13681 tgctacttct gcaagtagtg cttatgtgct aaagtaagaa tgaaaaggag agataaacat
    13741 gatatttgat tctcataccc atttaaatgc tgaacagttc aatgaagata ttccagaaac
    13801 gattcagcga gcacaagaaa tgggggtaac gaaaatggcg gtcgtgggct tcgatacgcc
    13861 aacgattgaa aagtctttgc aattaagcca tgactatcca aatatataca gtattatcgg
    13921 ctggcatccg acagaggctg gcagttatac gaaagaaatc gaaaataaat tacaagaaca
    13981 attgacttta ccaaaggttg tagcattagg ggaaattggc ttggattatc attggatgga
    14041 agacccgaaa gaagtccaag ataaagtatt tcgcagacag attgctatcg caaaagaaat
    14101 gaacctacca atcagtattc atactagaga agcacttgaa gacacgtatc aaatcctaaa
    14161 agaagaagat gttcgagata tcggtggaat catgcatagt ttcagtggtg attatgaatg
    14221 ggcaaaacga tttcttgact tagggatgca tatttcattt agcggtgtcg tcactttcaa
    14281 aaaagcatta gacgtccaag aggctgcagc aaatgtacca atcgaacgat tacttgtgga
    14341 aacagatgcc ccttatctgg caccagttcc ttttagagga aagcgaaatg aaccaggcta
    14401 tactcgttat acagtagaaa agattgctga acttcggaat cttccgacag aagaagtggc
    14461 tgcccaaaca tggacaaatg cgcaccgatt atttggtcta agcgaacatg gataagatga
    14521 agatccaaga gattatagtc gttgaaggaa aagacgatac aagaaggctg cgggaagtag
    14581 ttgatgcaga tacgatagaa acaatagggt ctgcaatcaa tgaagagatc ctgatgcaaa
    14641 ttgaacatgc ccaagagact cgtggagtga tcgtttttac cgatcctgat ttttcaggag
    14701 aaaaaataag aaaaatcatt atggaagctg ttcctcaggc aaaacatgct tttttgccgc
    14761 gcacacaagc taaaccaaag aaaaagggaa gccttggtgt cgagcatgca agtgatgctg
    14821 ctattttaga agcattgaaa aaggtcgtga cgccagacat ttcccaaacg gctgtttctg
    14881 aaatcactag acaagacctt ttagactatg gtttaatagc tggggcacaa gcaaaagaaa
    14941 aacgagaaaa actaggtgac gagttgcgca tcggttacac gaacggcaaa caattggaaa
    15001 aacgtttgaa gatgtttcgt attacgccga aagaactcgc ccaagcaatg aaaatagtgg
    15061 aggaacaaca tgagtgaata tcgtgaaatt gcgacaccct ctagaacaaa agagatttta
    15121 ttgaaacatg gtttttcatt taaaaaaagt ttaggacaaa attttctgac tgaaccaaat
    15181 attttaagga aaatcgtaga aactgcgcaa atagatgaac atacgaacgt gatcgaagtt
    15241 ggaccaggaa tcggtgcatt gacggaacaa ttagccaagc atgcaaaaca agtcgtagca
    15301 tttgagatcg atgatcgttt gatccctgta ttggctgata cgatgcaacc ctatgacaat
    15361 gtgactattg tacatcaaga tattttaaag accgatttgt caacagctgt tagagaaaca
    15421 tttcatgagg agcttccgtt aaaagtcgta gcgaatttac cttactatat tacgacaccg
    15481 atcatgatgc attttcttga atcagatttg gttgtggatg aactagtcgt catgatgcaa
    15541 aaagaagtag ctgatcgtat ctctgctgaa ccaggaacga aagcttatgg ttcattatcg
    15601 attgctgttc aatattatat ggaagccagt ttagcattca tcgtaccaaa aacagtattt
    15661 gtccctcagc caaatgtaga ttcagcgatt ttgaaactga caagaagaga tataccggca
    15721 gtcgaagtga cagacgaaaa agcatttttc cgattgacaa aagcggcatt tcagcaaaga
    15781 agaaaaacgc tttggaataa cttgcagcat tcgtatggca aagatgacca gacaaaagca
    15841 tggttagcaa aaagcttgga aacagctgga atcgatccaa aacgtcgtgg ggagactttg
    15901 tcattacaag aattcgccgc attaagcaat gcgatgtcag aaaatatgca gtaaaaaaag
    15961 aggttgtgac agaagttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca
    16021 aaaatagttc ctcatgtttt tcgtttgttt gggcttatta ccgcagatgc aagcttttga
    16081 acaccgttta agaaagaggt tgtgggcctg ccgtttaact ctaagtcaca aggaacaatt
    16141 tcaaaaaaca gcttctcatg tttttgagtt agttggtctt attacttgat gcaagctttt
    16201 gaccactatt atgccgaaca aggagatgtg acacaaattt tgtcacatct cctatttttg
    16261 tttcttgatc aaaaaattct tatacttgat actcacactc attttgtatg gatttagaaa
    16321 agaatatgtg tcttgacagt tattagtaag tgagttatac taagtcgaca tttatgtgtc
    16381 aagacatatt aggagagaga aaaaatgaga aaaaattcag ttcgaaaact aacgatttct
    16441 gctttattga ttgggatggg ggtcattatc ccaatggtca tgcctaaaat catgattcca
    16501 ccagcttcct ttactttggc gtctcatgtg ccattattta ttgcgatgtt ttttacacca
    16561 ggagttgcag tggctgttgc gctaggtacg acatttggtt tctttttaac aacgccggtt
    16621 atcatcgcat tgcgagcatt gtcacatctt gtgtttgcgt tgattggggc ttggtacttg
    16681 caaaagcatc cgagtatcgt tttaaaaaat ggacagttta cttttgctaa ttggcgtttc
    16741 caagggttca attttgtgat cggtgtgatt cattctttag cagaaatgct agtagtcagc
    16801 atcttttact ttattggtaa tatgcctgaa acatattatt ctcaaggata tttttataca
    16861 gtattcatct tgatggggat tggtgggctt atccacagtt tagttgatta taatattgct
    16921 tattttatag caggaacact cagcaaatat tttgatatcc ccgtatttac ggcagcaaaa
    16981 aaagaaaatc ggaaagagtc gattcaaggg tacgcgaaga aaacgacata ttagacatga
    17041 tctctcaaaa aaaaaactct catctagtaa tggataataa atcctctaag atgagagttt
    17101 tttgtcctaa aaagagctgc accaacatct tctggcacag ctctaacagg gggcaattta
    17161 tggaagtttg atgacttgtt ctgtttcttt atcaaagaag tggcctttgc tgatgttgaa
    17221 ggcaaggtcg actatttcgc ctggtttatg gaaatctctt gcatctactt ttgagatgaa
    17281 ctcagtagaa ccagtttttg tgtaaagcat cgtttcagct ccaagtaatt cggaaacgac
    17341 aacttctgat ttcactacgg cttcaggtga agcttcaagg gctacttgtt cactgtggat
    17401 gtcttctgga cgaataccga agataacttc ttttccttca taaccttttt caactaatac
    17461 tttgtttttt ccttcaggaa tacgaagctt aagcccatgt ccatctgtga taacaccatt
    17521 tttcaatgtc acattaaaga agttcatggc aggagatcca ataaagccgg caacgaacat
    17581 attgacgggt gtatcataaa cttctttcgg tgtaccgatt tgttggataa atccgtcttt
    17641 catgataacg attcggtcag ccatagtcat tgcttctgtc tgatcgtgag taacatagat
    17701 ggtcgttgtt tcaagacgct ggtgaagttt ggcaatctca gcacgcatgg ctacacgtaa
    17761 tttagcatct aaatttgata atggctcatc cattaagaag acttttgcat cccgaacgat
    17821 tgcccgtccc aatgccacac gttggcgttg tccaccagat aaagcagctg gttttcgttt
    17881 caagtattct gtcagaccta agatctctgc tgcattgtcc acacgttttt tgatgtctgc
    17941 tttatcatat ttacgtaatt tcaagccaaa agccatatta tcaaaaacag tcatatgagg
    18001 atacaatgcg tagttttgga aaaccatggc aatatcacgg tctttaggtg ccacgtcatt
    18061 catgactttt ccgccgatat ttagttctcc ttcagagata tcttccagtc ccgcgatcat
    18121 tcgcaaagta gttgactttc cacaaccgga aggaccaaca aaaacgataa attcacggtc
    18181 tttgattgat agattaaaat cagttacaga ataattttct gcattgtcat attttttata
    18241 tacgtttttc agagccattt ctaccataat acgttccaca ctttcttttt tagttttatt
    18301 aactatttaa agtataaatg aaagcgttaa ctaacttcaa tgtcatgttg aacaagaatt
    18361 ttttcttttt tttgtcaatg tgctagtttt aagctttaat aaatatccat aagaaataaa
    18421 aactgataca ctggagtaaa taagtatgtt aacaatatca aaaggagtgt ctcttatgaa
    18481 aatcgctttg atcgctcatg accgaaaaaa acctatgatg gtgaaattag caacagcgta
    18541 taaaacaata ttggaaaaac atgaactttt tgctaccggg acgactgggc agaggatcac
    18601 ggaagcaacc ggcttgccga tccatcggtt taaatccgga ccgttaggcg gggatcagca
    18661 aattggagcc atgatttcgg aggatcagtt agatttggtg atttttcttc gagacccgct
    18721 ggctgcacag ccccatgaac cagatgtaac agcacttatc cgtttatctg atgtctatga
    18781 gattccgtta gctacgaata tcggaacagc tgaggtgcta ctaagaggac tggaagccgg
    18841 cttcctagat tttcgaagaa tcgtccatga aatagacaag catccacttt cattttgatg
    18901 gataagcgta tggaaaaata acgtttaatg ttcgcctata tgtagctata aaagctaaac
    18961 aaactgataa tatgcgatat aatacgaacg tattggataa tataaacaat aatattagtt
    19021 tttcgtattt gcagtaaatg tgaaaatatg ctatagtttc tgtgggaaaa gatcccaaaa
    19081 ataatacaga gtagaaaata aagcgttaag tgctaagagg atgggatgtt gcctcttgga
    19141 cgaaggacgt aatgttcgcg gttttatttt cgcattcgct gcatgatacg atgtagcagc
    19201 tcttaaagga gatgctaaaa atgaagaaac accggttaga cgcacgtatg attgctatta
    19261 tgggcttatt gattgcattg atggtcactt tgtcccgact ggtagcaatc gagaccccat
    19321 ttattaaaat cagtgtcaca tttattccac aagtgatcat gggcattctt tttggtcctt
    19381 tttggtcagg gataggcgca gttttagctg atttagtagg aatggccctc ttctcgaaat
    19441 ctgcattctt tattggattt acattgaatg catttattga aggagcaatc tacggattct
    19501 ttttctatcg aaaagagatc acttggaaaa atgccattct ggctacgctt agtgtcacgc
    19561 tgatcatcaa cttatttttg acaccattat ggcttgcgtt gatgtaccat gtgccattgt
    19621 tcagctgggt cgtttgggcg ccccgcctgt taaaaacagt aatatggtta ccgattcaat
    19681 caattgccat ttattacgta ggacgtagta ttccttacaa aaaaatttta agaagtttag
    19741 cgatccacgc taaataaaaa acaagcagtc ttcccatccc agaagactgc ttgtttttat
    19801 tgtgctgtga attgtgcttg atagagccgt cggtaataac catctttttg aagcagctca
    19861 tcatgggtgc ccatttcgac aatagctccc tgatccatca ctaatatctg gtccgcattt
    19921 ttgattgtgg acaggcgatg ggcaatcaca aaactggttt ttccttccat cattgctaag
    19981 aaggcttgtt ggattttttt ctctgtcaat gtatcgactg agctggtagc ttcatctaag
    20041 atcaacatag ggggattact gatcatcgtt cgagcaatcg tcaacagttg gcgttgtcca
    20101 tctgatatct ttaacccttg accgccaatc ttagtatcta atttttccgg caaacgttct
    20161 acaaattcat acatataaga ggcttttagg gcatcattga tttctgcgtc acttgcttcc
    20221 ggattcccaa aacgaagatt ttctcgcaat gtactgtcaa atagccaagt atcttgtaac
    20281 accattccaa aacttttacg taatgtatca cgttggatag agcggatgtc atggtcatcg
    20341 actgtaattg ttccttttgt tgtgtcataa aaacgcatca gcaaattaac aagtgtggat
    20401 tttccggccc ctgtttttcc tactatagca atggtttgac ctggataagc agtaaaattg
    20461 aaatcttcga tcaaacgctg cttggggtca taagaaaaag aaacatgatc aaaagagact
    20521 tttcctttga tatttttggg ttccaaggca taggcatctg gcaaatcagg cttttcttct
    20581 ggctgatcga tcaaatcaaa tgctcgttct agaccagaaa aagcggtttg cagttgtgtc
    20641 gtgattccag aaagttcgat aaatggttta gaaaactgac ttgagtagat cgtaaagctg
    20701 gaaatcacac caacagtgat tgacgaacca ctcttcaatg ccaataggcc gccaactaaa
    20761 ccaatcgata aataagcaag gtgatctaca aaacgagaag acgggtttgt caaggaagaa
    20821 gaaaattgtg cccgttgtcc tttttcatat agatcagcat ttagcagttc aaattgctgt
    20881 tgactagctt cttcttgctg aaaagctttc acgattttct ggttaccgat tcgttcggtc
    20941 acaaagcccg agatatcccc aataatcctt tgctgtctat cgaaatttgt ttgtgaagca
    21001 cgagcaacga tccaactcac aaggaaaata attggtgtag aacaaagaac gaccaatgtc
    21061 agaagtggac ttagacgcat cataaaaata aacgaaagaa tgatgattgc cactccagaa
    21121 aataattgat tgaaggccgc agaaattgca atggaaatat tgtccatatc atttgtaaat
    21181 cgactgacga tatttccgtg ggagttttga tcataataac gaagtggcaa tcgattcaga
    21241 tgagcaaatg catcttttct taattttgca acggataagt aagctaaccg attgcttaac
    21301 acttggatga gccattgact gactacgttg attaaaacga taccaccgaa tagaataagt
    21361 aaatggaaca gtcgattgaa gcggacctgt ccttctccaa tcatttgatc gatactttga
    21421 ccaatttgta acgtcatcac aactgttgca atcccattga tgattcctag tatcacagct
    21481 agccaaatgt cttttgcata ggcagtaagg taaggaatga atcggcggag cgtctgcaag
    21541 gataattttt ttactttcat aactattctt cctcctgtga tgcgacgatt tcctgatatt
    21601 ctgctgattg agcaagtaat tcttcatggg taccctgagc agcaatctta ccttgatcca
    21661 tcactaagat atgatgtgct tcttgaatag agcggacacg ttgggagata ataatcactg
    21721 tactgccaag ataagtctgt agcgccttac gcaaatccaa gtctgtttga taatccaacg
    21781 cacttaaaga gtcatctaaa atcagtaatg ctggcttggc aatcaaagct cttgcaatcg
    21841 tcagtcgctg tttttgtccg ccagagaaat ttttcccatt ttccatgact tttgtatcta
    21901 aaccatttgg aagctgacgg acaaattctt ctgcctgagc gattttcaga gcttcccaac
    21961 attctttttc tgtggcgtct ttcttacccc attgaaggtt ttcacgaata gtcccggtaa
    22021 atagtacaga agtttgggga acaagagcaa tttgctgacg gagatgatcc agctgccaag
    22081 aacgaacatc cattccgtcc attataactg taccggaact gacatcatag aaacgcggaa
    22141 tcaattgtgt caaagtcgtt ttcccgcttc cggttggacc aatgattcca aggactgtat
    22201 ttcgcttcag ctgaaatgta atattttgga gagaaagacc agaatcagga gtataacgga
    22261 agtcaacatg gtcaaaagcg ataattgctt cagaagaaaa atcgggagta gtactgacct
    22321 cagcttcaag aatcgaattt tctgtgtcta gcacctcttt cacccgattt cctgaagctt
    22381 cggcacgtgt gaagatcacg acgaggtttg aaacaacgat cagtgccaaa agcatttgat
    22441 tcatataatt gatcaaagca agaacttgtc cttgttgcaa gctaccaata tttactttga
    22501 tcccgccaac tgtcagcaaa cagatgatcc cgacattcat gatcaaggtt gtagcaggtg
    22561 ataatagggc agagatattg gaaacacgta aataattgtt cgctagatca tttgtgcttt
    22621 tatcgaactt ctctgtttct ttttttgtct gcgcaaatgc gcggatgaca cgaacaccgc
    22681 taagattctg actaacgtgt tcattgagct gatctagttt ttcttgcact ttttggtaga
    22741 gaggaacggt ttttttgatg ataaaaaata aaatgattga gaaaataggc aataatgcta
    22801 agaaaaacag acctacttcc cagtcgatca caaaagccat gaccactgac ccgatactta
    22861 aaaacggtgc tctgatcaat aaacgaatga ccattgctaa agctagctga agctgattga
    22921 tgtcgtttgt cattcgggtg atcaaggtat ctgttccaaa atgattcaac tcgttgtgag
    22981 agaaagtatt gatttttttg attagctgat tacgtaactc tgtaccaaat ccttgtgaag
    23041 ctactgaagc atagtattgg caaatgatag cacaaatcag accaatcaca gacatagcga
    23101 ccatccataa gcccatttca atgatttttc ctcggtcatt catttttagt ccattatcga
    23161 tcaaggaagc catcattaaa ggcaggacaa gttcgaagac agcttcaaga aatttgaaga
    23221 acgggcccaa aatgatttgc ttacgatagt cttttgcata acgtaataac tgaatcatat
    23281 gattcctcct attatttatt tgtccacaat tgaaaaaaca agtactacca cattgtagct
    23341 taggaaattt agaatgaaaa gagaacaagc cttttcataa atgaaagttt tattatgaag
    23401 aagctttaga aattgaatag actatttttt ttaaaaaaaa gaaaatgaga aaacatgcca
    23461 gtctcttcat tcataggaac gtctatttta cttaattttt ctataaatga attctgcata
    23521 aaaaatatat caagtaaatg tttatagatg aaactctatt tacttctttt ttaatttgtt
    23581 cttgcaatct tttttgctac gtaatttttg taaaaaagtg ggttgagaga aatctaattg
    23641 attttcaaag aaggagtatg aaaatgagaa aaatatttat tcgtttattg tgggtactat
    23701 cttagaggaa taaataatag tggagaaaac tatggtttgg cgcagattct atcaggtttg
    23761 atggaacgaa gacaacctcc cgacatcaag tcaaaagcta cttgagattt tgatctgaaa
    23821 ataaaaaaag aaaacaaggt aggaacatca atgtttctac cttgttttcg taatcaatgg
    23881 gaaaaaacta agagtggtgt acgtaatcaa taaaaaggat ggtgacaaca gtacctcttt
    23941 actctctgaa agcatcataa ctcgtttttc ctcattatgc caataaattg ttgttatacc
    24001 acatataagg atttgtaata gctaaaaaca gacaggaaag ttgaaatctg tgaatcttat
    24061 tttgtatgga aggatttata gaacgcaaaa atcaagataa agatgaataa aaatcctgtt
    24121 tcggctatat attgcgtatc tacgccaata aaacgagttt gttctgtcat agccatataa
    24181 agcaaaggca aaaatacaac tacagcgaga taagatccga tgatttctcg aaaagttttc
    24241 caggaagtat gtttcctgtt tttatattta tccgatttca ttgaatagtc atagcctcct
    24301 tattctttca tatgttgaaa ttccagtata aaaactggaa ggaactgtac gttttttaga
    24361 ataaaaatta taagggataa gtaggctgct gtctataata agatatattt ttcaattctt
    24421 tattaaagat tttattgaca aaatgaatcc gttttctatg atggaggagg ggtgaataag
    24481 ttgttgaaga aggaaatttt atgttactta gagaaccaga cagccttttt cgatttagaa
    24541 catatgaacg aggtctttac agcacaaagg ttagctgatc attttggggt aaaaagaaac
    24601 acagtcagtc attatttgaa ccagttaaat gatgagcaac tactagtcaa aatcaattct
    24661 cgaccagtga tttatttcca caaagaagct tttgaaaaac aattttttaa gttacgcacg
    24721 aatttttatt tttctatcga tgatctcaaa gaggaacagc cttttttcgc gcaaccaaaa
    24781 gacttattct cttttatgat cggatacgac gccagtttga aagaaagtat cgaacaaatc
    24841 aaaacagctc tttactatcc agatggcgga ttaccacttt tgattacagg cgaaagtggc
    24901 acaggtaaaa gttatttagt taatttggtc tatcagtatt gtctgcttca tgatctgcta
    24961 gaagattctg caccattcat cacttttaat tgtgcacaat atgcagataa tcctgaactt
    25021 ttgactagca atttgtttgg acatgttaag ggagcgtaca ctggtgcaga agaaaataaa
    25081 aaaggggctt ttgaagcagc agatggtggg atcttgtttt tagacgaggt acatcgctta
    25141 acaccagaag gtcaggaaaa actgttcacc tatcttgatc aaggtgtgat ttatcgtatg
    25201 ggagaaccta atttgtcacg ccggatcaaa acacgtctct tttttgctac gactgaagag
    25261 attactagtc atttcttaac aactttcatt cgaagaatac ctatccgaat cgaactacct
    25321 gcacttaatc agcgtagtcg taatgaacgg ctagaactga tttactcttt ttttctcgaa
    25381 gaacaaagaa aaatggggag ccctttgtca gtcagtggac cagctttgtc acttttaaca
    25441 agtcagaaat tatccggaaa cataggagag ctgaaaaata tcgtcaaagt ttcagcagca
    25501 aaagcatatg ctgaacagcg tgaacagcct gaaatccatg tcacgatcca tcatttgcaa
    25561 aaagaactac ttgcgcagcc tattactaaa aatacagcac agcaaggaat ctatattacg
    25621 caagaccaga ccttagagca attaatggag aagcaacagc ccgaacagca gcgaatcgtt
    25681 aaaagttttg agcaaatcct aattgatttc aagaaaaata actgtctgct atattcttgc
    25741 gaaggaaaat taaagcaaga agtgattcaa ttatttgatt ttttgctgtt tgagaccagt
    25801 agacaagaaa aacacgagct gctggtttat tggacacaat ctatccgaga gacctttcga
    25861 caaatggaaa ctgcctatca aatcaaattc gatggaaaca gtgtctatgc cttgagttac
    25921 tatctctatc agcgaagcac tatcaaatgg ttaccggaag aacatgatat tatccaacta
    25981 atcaaagaat tagaacaaca agtttctcag tcttatccat ctagttatca ctatgtccaa
    26041 cgaattctgg aattaagtaa gatcaaatta gatattgaag acacaagtat ggatcggatt
    26101 ttactgacga tctatctaaa aaaagcgaaa tggtcaaaag aaaaacgact gccaaaagcg
    26161 atgattgctg ctcacggtta tgcaactgct agcagtatgg caaatgtagt aaatcgttta
    26221 ttacaagagg atctatttga atcatttgat atgccactag atgtcacacc acagcagatt
    26281 gcttcagaaa tccttgatta ttgcgaaaac agcgatgttt caaatggatt gatcatatta
    26341 gtagacatgg gatctctgaa agaaatccat cagtttttta aaaaacagtt atccgtaccg
    26401 cttttgattt tgaataatgt gacgacacct ttagcaatca cagtagggga atgcctgcaa
    26461 aaaaatgtat cattagaaga gatagcagaa gagactgttc gacagatcca gccagaatgg
    26521 cgactgctct atccagagga aaacaaaccc aaagcactca ttactacttg cttcactgga
    26581 atcggtacgg cggtccactt aagtgaactg ctagaaaaaa gcttacctac ggcttgtcag
    26641 ttgaaaatca tcccatatga atatcagcaa ttaaaagata agaaaaacag tgacccatta
    26701 ttttccatat acgaagtcgt aggaatgatt ggcaccacag atcctgatat caccaatatc
    26761 gactatcttt cattagaaga cctcatctcg ggggaaaaga tgtctgtact agctgaatgg
    26821 ttggacacta ctatgaatgc ttcagagaag gaaatattca atcagcgcat cattcgtaat
    26881 ttttcattgg aaaaagtact ggattccgtg actattctag atacagagaa ggtgatggga
    26941 gaaatcgatc attttatgag agaattagag gtgcagttga atcgaagcat cagcaatccc
    27001 aaaaaattag cgttgtatgt ccatgtgagc tgtctcattg aacgattgat ccgacaaatg
    27061 ccaatcgaaa cctatcaagg attagagaaa tggaagcagt gtcaaaaaga agggttatct
    27121 gctattaaat ctgcgtttag tgtcattgaa gagcattata gtgtcatgat ccccaattct
    27181 gagattgcct atatctatga tattttgagt gaagatacag agtttctctc gatagaagaa
    27241 gaattttgaa cataagcaag ttgttgccac taattttggc atgcaacttg cttttttagt
    27301 atgtgtaagt gaaaagagga gatgaaaaag tggatagaga aattatctta gcttcccatg
    27361 gaaaatttgc ttcagggata ttagattcat tagagctgat ctatgggaaa aatcattcta
    27421 ttactgtatt cgattgctat actgatgaga attttgattt agcggaaaca gtaaggcagc
    27481 tttttatgag gtacaaagat aaagaaatca ttgttctgac ggatcttttt ggcggaagtg
    27541 tcaacaacga attcttacaa tatatcaatc gggatcatgt ctatctcgtt gccggattga
    27601 atttgccact attgattgag ctagtatctc ggttggacgc tgaagtagag acaactgttt
    27661 tgatccagca ggtgctaact gattcaaaaa aaatgatcca gttttgtaac gagcggctca
    27721 atcagaaaat agaagaagac gaattttagg aggaagaaaa atgattgtat tgacaagagt
    27781 cgaccatcgg ttattacacg gacaagtagc tttttcatgg acacaaacaa taggcgcaga
    27841 ctgtattttg attgctaatg atgatgtacc tacgaatgag atacgaaaga caacgatcaa
    27901 attagccaag ccacaaggag tcaaactagt gatcaaatca attgatgact cgattgccgc
    27961 gttgaaaagc ggggtgacag ataaatataa gttattcatc gtagtggaat cgatcgagga
    28021 tgcatataag ctggcagaag cgtatccaga aatcaaaacc atcaacttag gagggatcaa
    28081 ggcaaaacct ggtactcgca gcatcggaaa agcggtaaat attttaccag aagaggagga
    28141 acgcttgaaa cgattaattg acaaacaaat agaagtagaa atccgtcaag taccagcaga
    28201 taaaaaagtg gatgtaagag aagttttgta agaaaggaag aacctcaatg attgtacaag
    28261 cagtcttatt aggaattgtt gcatttgtcg cacaatcaga atatgcacta gggacatcgt
    28321 tgctttctcg tccgattgtg acggggctgt ttacaggaat cgtgttaggt gatgtaaaaa
    28381 ctgggatcat tatgggcgca actttagagt tagcatttat cggatcgttc tcagtaggtg
    28441 cttcgattcc acctgatgtt gtgaccggtg ggattttagg gacggctttt gctattacag
    28501 ctggagccgg tacagaaacc gcgcttttac taggtttgcc gattgctacg ttgacattga
    28561 tattgaaaaa tatttactta gggcttttga ttccaattat gaaccataaa gcagacaatt
    28621 atgcagaaga aggaaattat aaaggagtcg agcatatgca cttgcttgcc ggtatcggtc
    28681 tttccttgat gcttggtgtt gtggtaatgg tttcttatat ggtgggaagc aatacgatcg
    28741 gaaatctatt aaatatgatt cctgacttcg ttcaaaaggg gttgtcagta gctacagggt
    28801 tgattccagc tcttggtttt gcgatgcttg ctcgtttgct gatcaacaaa caagttgcac
    28861 catatttttt cttaggtttt gctatagctt cttacttaga gattcctgtg accggagtag
    28921 ctatttttgg tgcgatttta gcagttgtgg tagtcaatat tatgaatgtc cgtcaactac
    28981 cacttcaaac aactgcaggg gaggtagaag aagatgaaga attctgagat caaaaacagc
    29041 aaaataacga aaaaagagct aaatcacgta ttttggcgtt cttttcaaat ggagttttct
    29101 tggaattatg aacgtcagat gaatatggct tatgtttatg caatgattcc gattttgaaa
    29161 aagttatatc agtcgaaaga agaaatggca gcagctttga aacggcatct agagtttttt
    29221 aacacgaccc cacatatcgt tacactgatt ttaggaatca cggctgcaat ggaggaagaa
    29281 aataaggatg atcctgaatt tgatgtgact gcgatcgata gtatcaaaac atctttgatg
    29341 ggacctcttg cagggttagg agattctttc ttttggggaa ctttgcgatt gattgccaca
    29401 ggtgtgggta cctctttagc catgcaagga aatattttag gccctatctt gttcattttg
    29461 attttcaata ttccgcacat tctgtttcgc tatctagcaa caggttgggg atatcgctta
    29521 ggaacgggtt ttttgaaaaa gatccaggca aacggtatga tggaaagttt gacattaggc
    29581 gcgtctatta ttggactgat ggtcgttgga gcaatgactg ccacgatgat caccatcaat
    29641 attcctttaa aaatcggttc aggagaaaat gctgtgactg ttcaaagtat ttttgatgat
    29701 atcgttccaa atatcttatg tcttggagca tttggcatag tgttttatct attgaagaaa
    29761 gaagtgaaac cattaacgat cttgatcgga ctagctttct ttggaatttt tggttcgtgg
    29821 atagggattt tttaaaaaag gagcagagaa aatgtttacg atgcaagatt atatctatga
    29881 agaaaaggaa gtactaagta cgatcttgaa aaagaatgat ttttcgacaa gagaacatat
    29941 gaaaaaaaca gtgaatttac tgatcttagc aacgggttca tcttataacg cttgtttagc
    30001 agcgaagcca gcattggaat cttatggtga tcttactgtc gatattcaag aaccttttta
    30061 ttttttcaat tatggaaaac tttctccttc aatcgatact gtgattgctg tgtcacaaag
    30121 cgggaaaagt gcttctactg ttgaagcagt aaaaatgata caaaaacaag ggttacctgt
    30181 tgttgctatc acaaatgacg tgcaaagtcc acttgcttta gaagctgcac aaattattga
    30241 tttaggtgcc ggggtagaat cagtaggctt cgttacaaaa ggctattctg cgactgtttt
    30301 acagttacta ctcttaggga taggaattgg aataagtaaa aataaaatca gcaaaaaaat
    30361 agaacaagac tacatgcaac agttgcgcaa aataatcaac catttaccag cgattattca
    30421 aaaaacggaa gaattttttg atggatatca aagtttattt cgtttagcac agcgtttcgt
    30481 caccattgga tatggtccaa actggggaac agctaaagaa gcagagacga aattgactga
    30541 aacaatccgc gttccgtcac aaggatttga gttagaagct tatatgcacg gcccatacct
    30601 ggaggcagat gcttcgcatt tgctattctt tattgaaggg gaaagtgtaa ataaggaacg
    30661 ttcgcaaaag ttgcaaagat atatgagtcg gtacgttgga gagactctca ccattaccac
    30721 aaaaaaagca agaaacgaga aaacacttgg attagcaata gaatgtgatg aatatctgtc
    30781 tgtattagcg cttgtcgttc cgtttcaact attcgcttat aaaaccgcag tagctaaagg
    30841 tattgacttg aacaaaaaga tttttgaaga ctttgataca gtcttgaaaa gtaaattata
    30901 agaggtggaa atcatgctga aattcaatga agaagaacaa atcaaagcaa taaaaggcgc
    30961 actagcttta cgtccgcaag tagaagaaat catcgataaa ctttatgcgg aaaaatttga
    31021 cgctgtctat tatctaggaa tcggtggaac gtatgcttcc tcgatgcaag ctgtcactta
    31081 tatgaatggg aaaagcaact tgcctgtctt tgtacagcat gctgcagaat attacactac
    31141 tggaaacaaa agattaacaa aagattctat cgttattttg tcctctgtta ccggtacaac
    31201 tcaagaagta gtgaaagcag tagaacaaat caagaaagta ggagcgacac tgattggatt
    31261 tatcgataaa gcgaacagta agttgtctca gctatgcgat tttgtggtca cttatccagc
    31321 tcctggaaca gaacaaatca aattttttat ggcagcagat cgattgatgt atttgaatgg
    31381 agaattcgaa gcttattcag aatactatga acagctcgaa cgttatttac caacagggtt
    31441 agtggaagca gagaaaaaag cagatgcatt tggcttatca tttgcagaaa aacatcgacg
    31501 tgatagtatg cattatttta ttggagcagg gaatcaatgg ggagctgttt attcatatgc
    31561 catgtgttac tgggaagaac aaagctggct tccatcgaaa tctatccatg ctgccgagtt
    31621 tcttcacggg acgttagaga tcgtagaaga aacaacccca gtcacattat ttttaggcga
    31681 agatgaacag agagtgttat ctgaacgtgt ggctaaactt ttgccaaaaa tttgcagcaa
    31741 ttatacattg atagacacaa aagactatcc agtagaaggt atcagtgaaa aatatcgcgg
    31801 acgtgttttg tctttcttat tgatgcatgt agtgacacaa cgcatcgatg ctcatgtgga
    31861 gcagttgaac tgccatccat tagagatacg ccgctactat cgtcagtttg attattagga
    31921 tagactggat aaaaagaata taaaaaataa agagagcagc gataactgct ccctttattt
    31981 ttttaaaata ttggcatcaa ttacgttata aaacacattt tttgcattat ctacagttca
    32041 acttataatc tgtttaatgg gacatgtggg tgccaaccat tttttgttat gcaatagtac
    32101 tatttagttg ttgcatggat ccgtagtaaa agtccaagta caacgagttg cgccagttta
    32161 catagcgttt tgtatccaga atgttgacta aaacaaaagt tcctatgtca accgtcaaca
    32221 gagacaatct gtccgttgca ggacctgaca gatttagaaa tgttcaatct gtctcttatg
    32281 tattttaaat caatactcac taggagaagt ttcctaacct cctaatagta atgctggtag
    32341 taacattgtc aaaactgctt cctcttagga acaagcaaag cataacgagt gtaaaaataa
    32401 aatgtagact attgtaacat atcattgatt atttcaagta aaaacgataa aagaagtaaa
    32461 caaacactta aaaacaatgg aaattagtcc caactttaag aaatgtatac tataatggaa
    32521 attatcaatt atttatttgg aggaatatcc atgaaacaaa aaatcgcagt tcttggtcct
    32581 ggatcttggg gcactgcttt agcacaaaca ttggcagaaa acgggcatga tgtccgtatt
    32641 tggggaaatg tacctgaaca gattgatgaa atcaataagt atcatacaaa tcagcatttt
    32701 ttacctgatt tgacgatccc agaatcgatt atcggatata aagaattggc agaagcagtg
    32761 gataacgcag atgctatttt atttgttgtt ccaacaaaag cgattcgttc agtcgcacag
    32821 gaacttattg gtaaaatgaa tactaagcca atcattatac atgcaagtaa aggactagaa
    32881 caagatacgc ataaacgaat ctcagaagtt ttggctgaag aaattccaga agaaaaaaga
    32941 caggcaattg ttgtattatc tggtcctagc catgctgagg aagtggcagt acatgatatc
    33001 acaacaatta cagctgcaag tattgatcaa aaagcagcag cctatgtaca aaaactgttc
    33061 atgaatgatt atttcaggat ttatacaaat aatgatgtga ttggcgtaga aactggtgca
    33121 gcactgaaaa atatcattgc aattggtgcc ggcgctatcc atggattagg attcggagat
    33181 gatgcaaaag cagcaatcat gactcgagga ttggcagaaa tcagccgtct tggtgtcgcg
    33241 atgggtgcca atcctctgac atttatcggt ttgagtggtg tgggggactt gatcgttact
    33301 tgtacaagtg ttcattctcg caattggcgt gccggtaact tgttaggaca gggacataag
    33361 ctggaagaag ttctagaaaa catgggaatg gtcgttgagg gtgtggcaac gacaaaagca
    33421 gcagtagagc tagctcaaca gctaggtgtt gagatgccaa ttactcaaac aatctataat
    33481 gttttgtata atggagaaga tatcaaaaaa gcagcaaaag aaatcatgtt acgtgacgga
    33541 aaaatggaga atgaattttc tcttcgttaa atagatgatc aatagttaat cgtcgtttag
    33601 gaggaacagc aagtgaaggt caaaaaagca gtcattcctg ccgctgggct aggaacacgt
    33661 tttttgccgg caacaaaagc catggcaaaa gaaatgcttc caatcgtgga taaaccaacg
    33721 atccagttta ttgtggaaga agcattggat tcgggaatcg aagacatctt gatcgtaacg
    33781 ggaaaagaaa aaagacccat tgaagatcac tttgatgcaa atatcgaatt ggaaaacaat
    33841 ttgcgggaaa aggaaaaaac agaattactc gctttagttg aagaaacgac tcaagtcaat
    33901 cttcacttca tccgtcaatc tcatccaaga ggactggggg atgccgtgct tcaagcaaaa
    33961 gcatttatag gaaatgaacc gtttgtcgtc atgctggggg atgatttgat gaaagatgat
    34021 atcccattga ctcaacaatt gatgatggat tatgaagaaa cacaagcttc cgctgttgcc
    34081 gtcatgcgtg taccagaaaa tgaaacatct aaatacggaa ttattgatcc taaaacagat
    34141 agcgaaaagg gtcgttgcag agtaaaaggg tttgtagaaa aacctgaaat gggaaaagca
    34201 ccaagtaatt tagccattat cggacgttat ctactgactc ctaaaatatt tgagatatta
    34261 gaaactcaag aaccaggagc aggaaacgag atacagctaa cggatgcact tcagactctt
    34321 aaccagacag aggcggtcta tgcgagagag tttaaaggaa aaagatacga cgtaggcgat
    34381 aagttaggat atatgaagac aaatattgaa tatggacttc aacattcgga aattgggact
    34441 tcattaagtg catatattat ccagttgagt aaaaagctaa gaacagaaag ttaactggaa
    34501 ataagtggcc tgctgagaat gaaaggctat ttgttattaa agcggcagct tgtgcgtcat
    34561 ttgcgcaact gtcgttttag ttttttgact ctagatcagt tgagtcgtgg aaaacgaaag
    34621 atagaaaaag actttcgtga atagattcag aaaatattat gctaatggtt taattttttt
    34681 ttaagttgta taaaagaaaa tgattacata agagaaatga aagccaagcc agaagattca
    34741 tagtagaata aaaaggcgaa ttttttaatt ataaaaaaag gtgtgtgttc actatgggaa
    34801 aattagtcgc tgttgcacag tcgctgcttg atatactcaa gtggctgatg gaaggaagca
    34861 attggatatt tgtgttgcca ctattgctta ttagtctatt tatcatgttt cgcatggtca
    34921 gaaaaaaaga aacgtttctg atttttatct ggtatgcagc atccgttggg gttgctacct
    34981 tgatggttga attggcgtta ggtagttggg aagcttgcgt atttgcttta ttttttgctg
    35041 tactgtatct aatcatttat atcccatata tgaaaagaaa gtctcggata ggcagaaaag
    35101 gtagacatac aactactttt tctagtacag attctctcga acaaagtaga agaacgagaa
    35161 gaagacggta atatttagta ggtatacgca aaaaaagata aaataatgga taaactattg
    35221 agatgagaga tttatttaat tggtggaaaa aagctttttt cacttatata aaagaatctt
    35281 ttttaaagga atgcttgttg tatcgggata agccattcct ttttttatgt tatttatata
    35341 tatataaatc ataccaatgt ttttttgtat tttcttgctt gcacttggat aagagattac
    35401 ttacaataaa ttcagataga aacagctgaa taaaaaaagg ctccctgacc cttagttggc
    35461 gctaagaatc agggaatgac cagtcacaca catacaccct gaccgatcat tttgccatag
    35521 tcaatgcagt gcatcgacct aatttattgt acctaatcta gggtgaaaat tctaccccta
    35581 tgtagatatt ttccatggtc tgcacgttgt tttatttgct atgtcaaggt attgggcgta
    35641 ttcgttcaat gccttttttg tgtttgaaac agcaaagacc acatgtgtta ctgtctaact
    35701 actgtttttt tatgtacttc cttagattag gcagctgttt ctattcatgt caagtatagc
    35761 taggcgggag agataaatga gtttatgtta ttttgactat tatcaccaat tggaagagaa
    35821 attaaggata gaagagccgc tgatgcttca agctggcact tggatcgcga aacaaattaa
    35881 tcaaggtggt agattgcaga tatatgctag ccgcacacta agtggtgtag catttgaatt
    35941 ttgggatcaa accccggatc ttgtaccaag tatattgatt gaaaatccag ccggaggtgt
    36001 ctatgaaacg ctagaaggtg caggacaggc aattattgat cagctttctg tcaaatcaga
    36061 agacattttt cttcttcttt cgaatgaagg aagaaacccg gcaatcatcg aactagctca
    36121 gtggatcaaa gaaaatggtc atccattaat cattgttact ggatttgatt tgtcacgaag
    36181 tatcaaatcg caacatagca gtggtttgcg attatttgaa tttgctgatt tggttttgga
    36241 caaccacgcc aatttacatg atgcagtctt gactttgcca aatctagatc cagcgatctg
    36301 cggaacagcc tctcttgcaa cgatcttttt gctgcagcaa gttttgtatt ttacagcaaa
    36361 acagctagtc caggacacaa gtggaaaata gttatccaca tgttgacaaa gttccttaaa
    36421 aaaataatgt tccacataaa aatggagcgt ttgaaaaaaa gaaaacgatt attaatgagg
    36481 attaacaacg actacttttt ttgttttagt tattttaaga tggatcatct ttgattttaa
    36541 gcttgttttg tttaaagttt gttattttta taagttgttt cacagttatg agaaatgagt
    36601 tataagaata tgtagtgtca gaaaaataaa aagtagtgga aaaagagaca agaaagaatc
    36661 catatggatt ctttcttgtc tcttaataca cgttgatgaa agagtcttcc ccaaaactct
    36721 ttacaaagta attatagtac atttgttatc attattgcaa tgaagtttac ctgatataac
    36781 aaaattgtcc aacaggatac ctttttctaa tagaaataaa atacacaaaa attgaaggat
    36841 agataatgga tatttataag tgtttattat tttttttgca atgaaattca atttttcaat
    36901 gattgtaaaa tgaaaaaaac aggacgagat tttctctaat ctaaaaataa aaaaagaatt
    36961 ttaaggaaaa acagagaaat aaaatactta atataaatgg aaagagaaaa aaataatcat
    37021 acataggaga aaaaaaacaa aatagaaaaa agtgattttc taataaatta ttcatgtgga
    37081 aatgtcattt ttactgtata attcatttga ttatccattt tttattcatc aaaattactg
    37141 aaaaaagaaa ggaagaaata aatttgaaga aaacaaattc ttcaggaaaa ccgaaacgcg
    37201 acttcactaa attatcgaca ccccatacgt atgtcattat ttttggtgta gtcatctttg
    37261 catggatact gacttttgtc gttccagcag gaaagttcag cacgcaagat attgaataca
    37321 aagatgcaaa tggcgaaaca agtactcgta ctgttttacg tcaagattcc ttccgttatg
    37381 cttacgagct ggataagtcg tatgtgtttg atcaattaga agaactgcaa gatcatccag
    37441 cagaacgtga aaaactggat gttccggaaa aaggattgga aaaagtcatt gctgacggag
    37501 aaaaaaatct tacgcaggaa aaactagatg aaatctcctt gacagatgat gtgctttacg
    37561 atcagtacgg cgaaaatatc tatgatactt ctaaaaaatt gcataaaaca gccaaaatat
    37621 ggggaacaga tgattttggc ggctttggtt tcttaaattt tgtttttgaa ggactagtat
    37681 ctggagataa gtatggatct gctgtaggga tcgctgcatt gatccttgtt gttggaggtg
    37741 cttttggtat tattatgcgt accggagcga tcgacgcagg tatctatgcg tttatcagta
    37801 aaactaaggg actggagcgt ttagctttac cgctattatt ctttgctttt tcattcggtg
    37861 gcgctacatt cgggatggca gaagaggtaa ttccattctc aatggtgatg gttccatttg
    37921 tcattgcgtt aggatatgac tcgatcgtag cagtaaccgt gacttatgtc gcatctcaag
    37981 tcggaaatgc gacctcttgg atgagcccgt ttagcgtagc ggtggctcaa gggattgctg
    38041 gaatccccgt gttgtctggt gcgacattcc gattgattat gtgggtagtt gttactgcat
    38101 tagccgctgg ttatatgatg atatatgcag aaaaaatccg taaaaaacca gaaagttcgt
    38161 tgacttacaa atcagatgat tatttccgtt ctcatatcaa aaagacatct gatgaaaata
    38221 aaaaattcat gcttgggcat aaattgatct tattagagat gttagttgtc cttgtctgga
    38281 tcgtttgggg cgtgactcaa aaaggctatt acattccaga aatcgcttct caattctttg
    38341 tgatgggatt agttgctgga atcattgctg ttcttttcaa attagatgga atgaaagtca
    38401 atgacatcgc ttcttctttc caatctggtg cagctgattt agctggtaca gcaatcgtag
    38461 taggtatggc caaagggatt cttcttgtct tgggaggatc tgatgcatct gttccttcag
    38521 ctttgaatac gattttgcat gggatcggga cggcgttgac cggtgttcca gctgtcattg
    38581 gtgcttgggc gatgtacatc ttccaaagtt tgttcaactt ggttgttaca tcaaattcag
    38641 gacaagcagc tttgactatg ccaatcatgg ctccactggc agatttagta ggtgtatcca
    38701 gacaagtagc agttcttgct tatcagcttg gttcaggttt tatggacgct tttacacccg
    38761 tatcagccag cttgattggt gtgctaggtg ttgcacggat cgaatgggca aaatgggcaa
    38821 gattccaaat caagatgcaa ggattcctgt ttgttttagg aactattttc atcatgattg
    38881 ccattgcaat aggtctgcag tagtactgga gacctgtaaa taaaaaagta atccaaacag
    38941 tagataagga ctccaagaac cgtcatttta cggtaaaatc aaaggagtaa agatcctatc
    39001 gtttggatta ttttttaaga tcctatcatc agttatactt aaagaaagag gaaaaatgta
    39061 tgaaaatcat tcgacaaatt gaagtgtttg caccagatta cttaggaaaa atggatgtac
    39121 tgattgccgg tgggaagatt ttggctgtag aagaacagct agaaggcggc tacgaaggag
    39181 tggaagtaga agaactttca ggtgagggaa aagtactaac cccgggtttc attgactgtc
    39241 atttccatat tttaggtgga ggaggagaag gcggttatca aaatcgaacg ccagaagtca
    39301 cattgagcca attgactaca gctggtgtaa cgacagttgt cggttgtttg ggaacagacg
    39361 gagaaggtag agatatgact gcgttgatca gcaaagcgaa aggtctggaa gcagaaggaa
    39421 tctccactta tgtatacgaa ggttcgtacc gattgccggt aaaaacagta acagattcta
    39481 tcatcaaaga ctttttgaca attgataaaa ttatcggaat cggagaaatc gcagtatccg
    39541 atcaccgttc ttctcagcca agttttgaag agttcgctcg tgcagtagca gatgctcgtg
    39601 taggaggcat gttatccgga aaagctggga tcatcaatgt ccacttaggc ggaggaaaaa
    39661 gaaagctaga gctattgaca cgaatcgtag aagaaacaga aattccgatc acacaatttt
    39721 tgcctactca tgcaaatcgc acaccagaat tatttgaagc ttgcgtggca tttgccaaac
    39781 gtggtggcac gattgacttc actgctagcg aagatcctga tttttgggag aagacagatg
    39841 gtgaagttcg cttcagcaaa gcactgaaac gattgatcga ggaagatgtt tctttagata
    39901 atttcacgat gacctcagat ggacaaggaa gcttgcctta ttttgatgaa aacaatcatt
    39961 tcctagggtt aggagtagga agtgccaagg cattgctagt agggatcaaa gaagctgtgc
    40021 aaaaagaaag tatccctttg gaaatcgcct tacgagctat aaccagtaat ccagcacgta
    40081 tattgaaatt ggataaaaaa ggaaaaatcg aaattggtgc tgatgcagat ctgtgtatct
    40141 tggataaaga aacgcttgat attgatacag ttatcgctaa aggagaaata atggtccaag
    40201 aaaaagaagt caaagtatgg gggactttcg aaaaaagttt ttaatcaaca gctctaacta
    40261 tcttttttag aaaacgagcg ttctgtttgt ctgtatctgt tgttgatgtt accctgaagg
    40321 tagatcaata aacaaactaa gaggaggaag cacaatggca gatttaaaag gacgttctaa
    40381 agatatgaaa gataaagtag tcggtgaagg aaaagaagca tacggcaaag caacagacga
    40441 caagagcaaa gaaacagaag ggaaagcaca atcactaggt gctgacatca aagaaaaagc
    40501 tcgaaattta ggcgacgaag tcaaagacaa gttttctcga aaagacgaaa aaaaataaaa
    40561 accacttatt actaattgaa tcaagcaaat aaaaaatccg aaccatatct gcgagacaga
    40621 tatggttcgg attttttatt tgatcattat cctgtttttt aaacttcgat tttttgaaat
    40681 tttttatttt ttagttcaaa gacttcatca cagctttttg ctacttcaat ctcatgagtc
    40741 acgatgataa tacagcgatt ttgttcatgg gcgatttctt ggaaaagttt gacgatatct
    40801 tgtgaagttg tttcatctag atttccagta ggctcatccg ctacgattag ttgatgttca
    40861 cagcagatag cacgaacgat agcgactcgc tgctgctgac ctcccgaaag tttggtcacc
    40921 tgtttttttg ctaatatttc atcaattcct acacgagcca gtcgatctaa agcaacttgt
    40981 cgataattct cttgcttggt cttagtgatt tccattgctg ctatgacgtt gtctagagcc
    41041 gataaataag gaagcaagtt gtaagcttgg aaaataatcg agacatcatt tttacgataa
    41101 cttcttaacc cgattttgtt cagtgaacga tcttgatagt atacctttcc ttcttttggt
    41161 gcatctagtc cagaaataag cgaaagaaaa gtcgtttttc cagaaccact tgctcctaaa
    41221 atagcgtaca tttttccagc ttcgaaattc agttctacat ctttataaag cgcatcttgc
    41281 ggatcttgat accaaaatcc caattgttct gtccgtaaca tcaaattcct cctttagttt
    41341 aataggattt ttcttgggtt taatcttaat atccctatag aagctaaaaa gacagaaaac
    41401 agactgattc caaatgctac tccggctaat agtcccaatt gtgcaggttt gacattgatt
    41461 tccaattcgc tgacttgatc agaaacagca aatggattat tcgtggttcc ttgttgacca
    41521 tcgcgttgac cgccttgttc ttgtctgtta cctggcattt gtccaccgcc aggagcttgc
    41581 tggttttgtg tcgtggtttc tgtctgttgg tcaagtagct gctgtcccac ggcattcgcg
    41641 acgacgtttc cgctaagcga agcaactcct aatgcaagaa tcatacaaat agccacttcg
    41701 gtaaacaatt ggagaatgat tttacttctg gattctccta aagatagcaa gacgcctaac
    41761 tcatgccgac gctcccgaat ggtaatcata atgatcagag taagtatgat aataccggct
    41821 acagccacta gtaaaacaac atttttagaa aaacttgcca cgttgtttaa aggttccaac
    41881 attgactgat acatggaatc atttgattgt aagctaaatg tatctgtgtc gatcagcttc
    41941 tcagcttctt tgagaaactc atccatttta tctggatcag atagagtgta aatagcagaa
    42001 tctatcgtgt tatcgtcaga tgtcccattt agttcatttg caaaagtgta agaagtgtaa
    42061 agtgtattcg acggattcat gaagttgaat ttcataccca tactgccgct tgtttctctg
    42121 gattcataaa ttcctttgat cgtcatttca taagtggtat cttcatcttc agtactcgtg
    42181 attgtgaaag tatctccgac tgccaggtca tttgtttctg ctagagtaga atcaatgact
    42241 acattgtttg tgcctttgtc gctgtcatct attccttcac catccgtgat ttttgctgtt
    42301 ccatcagaaa aattactatt ttgtgcagtt gctgacacac cactgatttg aaagtctgct
    42361 tgagtcattt gcggattccc gcccattcct ccagcaggtt caccgcctgc atcagtagtt
    42421 tctgaactgt cttcacttga atcagaacta gtgatggcag ttattccatc ttttgccata
    42481 gctgaggcgg aactttcaaa ggaataactc tttacattat ctaattcagc tattttttcc
    42541 gcgtccgtta aggaaactgg tgtaagagaa aaactgcctg gatctggacg ttcttcagtg
    42601 gtagaaccgc ttgaagaagt ttgttcctgt tttttaaatg aagcttctcg attggcactt
    42661 aaagtcactg ttgctccaac ttctttttgt gcttgattcg ctgcttcttc tgcagcgctt
    42721 cgaatcgtga ggccagctag cacaaaaata agaatggctg tgaacacggc aaacaataac
    42781 aatgaacgtc cccattttac ttttgtactt ttccatgcac gtttcataaa attcatattt
    42841 tgcatccctc ctagtagatg catttgataa tagacaacct atcttaaagc tagcttaaat
    42901 cttatgctgt ttgcacacca tcaacatgag gttctgtata tataaaaagt cactatagtt
    42961 attgcaaaaa atgtggtaaa atgaaaaaag tatgtttcaa accgacgaag aagaaatgaa
    43021 agaggaatag caaaagaaat cctagatgct acagagggat ttttacgtgc atagttgttc
    43081 taacaattgc tgatactagc gatttagttt atttgttgta taataatggg gaacagctga
    43141 atacttatat aagtattctg gctatagcgt ttcgcaaaag atccattttg ttaatgaagt
    43201 atatccaaaa taaaatgccc atccaactgt cagaacgcgg atgtgctggt aattttcctg
    43261 ctgattctcc ataagttgag aaatagtgat tttgcatatg cttcattgga atggctcata
    43321 tatttagata aagaagaaaa tcgacatctt taaacttgga tgtcataaga agaggtgaag
    43381 tttgtgagta ccattaaaat tatcccatta ggcggcgttc gtgaaaatgg aaaaaacatg
    43441 tatgtcgcag aagtgggcga ggatattttt attttagatt gtgggctaaa atatcctgaa
    43501 aatgagttgt tggggatcga cgtcgtaatc cctgatttta cgtatttaga agaaaacatt
    43561 gatcgtgtag ctggtgtctt tttaacacat ggacatgcag atgcgatcgg ggcacttcct
    43621 tacttgttgt caaaaatttc tgtaccagta ttcgggacag agttgacgat cgagttagca
    43681 aaattgaatg tcagtcgtaa tgaagcagcc aaaggattta aggatttcca tgtggtagat
    43741 gagcatacag aaatagactt tggtgaaaca gtagtgagct ttttccgtac cactcatacg
    43801 ataccagatt cagtggggat caatctgaaa acaaaagaag gtagtatcgt ttatacggga
    43861 gattttaaat ttgaccaaac ggctatccca atgtatcaaa cagattatgc acgattggcg
    43921 gaaatcggca aagaaggagt cttagcctta ctaagtgaat cggcaaatgc agaaaatcct
    43981 cagcctgtag cttctgaagc ccaaatagca gatgaagtat ttgatacgat caaatattgg
    44041 gaagggcgta tcatcgtcgc ttgtgtagca agtaatttgc aacgagtgca acaagtattg
    44101 gatgcagcag aaaaagcaca acgaaaagtt gtcttgactg gtcaagattt cgaacgaatt
    44161 attcgcacag cgatgcgttt agaaaaactt caattaccaa gtgaagatct gcttgttacg
    44221 ttgaaagaca tgaaaaacta tgatccagaa gaactgatca ttttagaaac tggccgaatg
    44281 ggtgaaccca tcaagtctct gcaaaaaatg gcaaacggta cacaccgtaa tgtcaagatt
    44341 gaagaaggag atttggttta tatcactact acgccaagca tcgcgatgga aaccatcgtg
    44401 gcgaaaacgg aagatatcat ttatcgagct ggtggaacag tgaagttgat ctctgagaat
    44461 atgcgcgttt ctggccatgc caatcctaat gacttgcaat taatgatcaa tttgatgaaa
    44521 ccaacatatt ttgtgccagt tcaaggagag tatcgtgaat tagctgctca tgcagatttg
    44581 gctcatgcag tagggatgcc atacaaaaat atctatatta ctggacgcgg agacgtgctg
    44641 gaatataaga acaagcgtat gagtgttgca ggaactacaa gtgcagaaaa tgtaatgatt
    44701 gatggattag gcataggtga tatcggaaac attgttttac gtgatcgaaa aatcttgtca
    44761 gaagacggga ttttcgtagc ggttgtcact atcagtcggc gagaaaagaa aatcatttca
    44821 aaacctcaaa ttacttcacg gggattcgtt tacgtaaaag ctagccgtga tctgatgaaa
    44881 gaaagcagca atatgattga aacaatcgtt gagaaacatt tagaaagcaa tgattttgag
    44941 tggagcaaat taaagcaaga catccgagat caattaggcc gttacttatt tgaacaaaca
    45001 aaacgccgcc cagtgatctt gccagtcatt atggaagcta ctcaacgtcg aaataaaaaa
    45061 taaacaaaaa gagtaagatg aacctctcta tagagtgctc gtcttgctct ttttttgcga
    45121 aaaacaaata atttttgaat acagcatccc tcgctttata ataaacgata gaataaaggg
    45181 agtgaatcaa tgatgagtca aacaaaagtc gaacgttatt tagaagaaca aaagatcagc
    45241 tatcaaccac ttgattttag tcatgtggta gaagaatctt tcacagaaga actacaaaaa
    45301 agagggattg atccagcttt gatatgtaaa actctcgtag tgaaaggaaa caaaactggg
    45361 gtgatcatcg cattgattcc tttgaacgat cacttagatt acaaaaaaac gagacaagtg
    45421 acgaaagatc gtaaagttgg ttttcctgga atggattttg tattaatgca tacaggatat
    45481 ccccacggag caaacacccc tattggaata aaactcgcac atcctgatta ttgtttttta
    45541 gcagacagtt ctttaaaaga aaaagaagaa gtaattgtgt caagcggtga aatcgggaga
    45601 agccttcgga taaaagtagc cgatttagaa aagatcgtcc atcctattta cgctgattta
    45661 attagttaag ggacgagaag agaagctaag cagagcagag acctcaaact tgagattata
    45721 tctgcttggt acttggtgca tcctgtatta tctgttacaa ttgctttgta catactgtac
    45781 tatcaatcca atgaaaatat atttagcagc tatatttttt cattgatgtg caagtgatga
    45841 aatcgttttt aataaaaaag atacagagaa aacgcgaggt gtaaaatatg aaaattacgt
    45901 atcatggaca ttcctgttta acaattgaaa tggaaaatgg ccaacgcgtg atgattgacc
    45961 cgtttattac aggaaatcca ttgacagact taactgcaga agaagtaaag acagattggt
    46021 tgctgattac tcatggtcat agtgaccaca taggagatat gattcctatt gcaaaagaaa
    46081 atcaagccac aattatcagt attgttgaga ttgctgatta tgcgaacagc agaggagtag
    46141 attcattcgg tatgaatatc ggtggaaaac atgcgtttcc attcggcgtg gtaaaattcg
    46201 tacatgcaca gcatagttca agctatgaag tagacggtat cgttcagtat atgggcgagc
    46261 ctagcggaat tattattcaa gcagaaggaa aaacgattta tcatgcaggg gatactgctt
    46321 atttcagtga tctaggattg cttgcagaag aattcgatat tgatgtcgct ttcttgccga
    46381 ttggcgataa ctatacgatg gggccagaag atgcagcaaa ggctgcgcaa acaattcgtg
    46441 ccaagaaagt agttccaatc cattacaata ctttccctgt cattgaacaa gatcccgaat
    46501 cttttgtaga gttactggaa gatagtgaag gaaaaataat gagcgtctcg gaaacgctgg
    46561 aagtgtaggc agaaaatggc gacaaaacat gatttgattt tagaatatat tgaaagttta
    46621 cctgttggca atcgaatttc tgtaagaagc atcgcaaaag aattgaatgt cagtgaagga
    46681 acagcgtacc gtgcaatcaa agatgcagaa aatgtcggac tagtttcaac aattcaacga
    46741 gtagggacca tccgtatcga acgcaaatta aaaaaacata ttgaacgttt gacatttggc
    46801 gaggtcgtca ggatcattga aggggacgtt ttaggcgggg cggcaggatt agacaaggtc
    46861 ttgaataaat tcgtcatcgg cgcgatgact aaacatgcca tggaacgcta tattacgcca
    46921 ggatcactaa tgatcgtcgg caaccgggcg gaagtccaaa aattagcatt ggaagatggc
    46981 gccgcagtat tgatcacagg aggattcaat acgactgctg agattgcaaa gctagctgat
    47041 gatcttgaaa tgccgatctt acgcacctct tatgatacat ttacagtcgc aaccatgatc
    47101 aaccgagcgt taagcgacca attgatcaaa aaagatatca tgcttgtcag tgatatctat
    47161 atgccagctg aaaagactca ttatttgcag cagacagata cgatttctga ttatcagcgt
    47221 ttgtcagaag aaacgcagca ttcgcgtttt ccagtcgtca accgccatca tcgtttgatg
    47281 gggattgtta cggcaaaaga tgttttaggg aaatcgccaa accagatcat cgatcgggtg
    47341 atgacgaaag aaccaatcag tgtaaagaaa acaatgagta ttgcctctgt tagccatcaa
    47401 atgatctggg atgggctaga agtcatgccg gtcgtttccg atgacttgac attggaaggc
    47461 ttgattaccc ggcaagatgt aatgaaagcg atgcagcttg tccagcgaca accgcagatt
    47521 gcagatacga tttcggatca gatatctgga aatatccata cgattgatac tgaccgtgaa
    47581 ggaaatcgtt tggaaaaccc taagttcaaa ttcgtagtag caccacaaat ggtcaatggc
    47641 gttggaacca tttcttttgg cgtacttagc gaaatcattt ctgatgtcgc acaaaaaaca
    47701 atggtgatga accaaaaacg aaatatcttg attgaacaag tgaacctaca ttatttgcgg
    47761 ctgatccaat tggaaagcga attagacatt cgtccacgtg tattggaaat cggcagacgt
    47821 tccgctaaac tggatatcga agtgtttatt gaaaacacca tcgtttccaa agcaatcgtt
    47881 gtttgtcaag taatggaacg ttcttagaaa gcaggagtga aatgacagta cagcaagaaa
    47941 tagtagatac aataaaaaaa tacgataaaa tcattatcca caggcatttg cgccctgatc
    48001 ctgatgcatt aggatcacaa gtaggattag ccgaactatt acgggaaagt ttcccagaaa
    48061 aagaaatacg tcaagtaggt gaaacgattg aagggcttcg ctttttagca gaaatgcaag
    48121 aagttgaaga tgtcttctac caaggagcgc tagtcatcgt gactgataca gctaattcac
    48181 ccagaatcag tgatcaaaga tatcagttgg cagaaaaact gatcaaaatc gaccatcatc
    48241 ccaatgatga cccttatgga gatcttgtgt gggtaaatac aaaagcaagt agctgtagtg
    48301 agatgattgc agaatttgct ttgatgtttc ctgaatcttt aaagatgaac gttaaggctg
    48361 cacggttact ttatgcagga atcgtcggag atacagggcg ctttttgtat ccctccacga
    48421 cgtcccgtac attagagatt gcttcgatat tgagaaatta tccatttgat gcaagtgctt
    48481 tgaacagaga gattgaacaa atgccgatga aagtcgctaa actttccggt tatctttatc
    48541 aaaatatcca ggtggatgaa aatggtgcag gaagaataat ccttccgcaa gaaattcttg
    48601 ataaatatgg aatcgatgat tcagagacag cttcgatcgt ttcacttcct ggagtgattg
    48661 acgaagtgct ggcttggggg atttttgttc agcagccttc agggtattac cgcgtacgtt
    48721 tacgttctaa ggggccgatc atcaatgaat tggctaaacg ccatcatgga ggaggccatc
    48781 cattagcaag tggtgcgaat gcaaaagatt tagcagaagt agaggaaatc tataaagaga
    48841 tccaagatat atgccaatcg tataagagca gaaaaacaga atgatacttc gttcgaaaac
    48901 gtgccttttg tagtaaaaat cacaaatgta tcttttcaag atcttctttt tccgttacaa
    48961 tagtgctcac taaagatttt aaaggagaaa gaagatgaca ttaccaaatt gcccagaatg
    49021 tggctcagag tacacatatg aagatcgagg cttgttgatt tgcccagaat gcgggaacga
    49081 atggaacaaa gaagaacaag aaactgtcat agaagacgga ttagtggtat tagatgcaaa
    49141 tggaaaccgt ctgttagagg gcgatagcgt gacagtgatt aaagacttga aagtaaaagg
    49201 tgcgtcaggt gccattaaac aaggaacaaa agtcaagaac atccgtttag tagaaggcga
    49261 tcataatatc gattgcaagg tagaaggatt tggaccgatg aaactgaaat cggaatttgt
    49321 gaaaaagaat taaagaatat tcaaggtctt ttcacattct tttcctacta ctaatgataa
    49381 aataaagtaa acgaaaagga gtgaaacgaa aatgtatggt tatggaatgt atggtttttt
    49441 agatccgaca tttcttttgg tcattcttgg attagttatt tccggaattg cgtcagcgta
    49501 cgtcaatagt actttccgaa aatacgatca agtgagaagc aaaaacaatg tgactggtac
    49561 acaagcagca caatatatat tacaaagtca acagataaat aatgtcggtg ttcaacaaat
    49621 tgccggagat ctcacagata actacaattc aggtaataag atgttgagtt tgtctcaagc
    49681 aactgcacag tctacttcag ttgctgcgat aggtgtagca gctcatgaat gcggacatgc
    49741 ggtacaagat gcttcgggtt atgtcccatt gaagattcgt gcagcacttg ttccagcagc
    49801 taactttggt tcgatgattt cgttcccctt gattatggta ggtgtattgc ttagctggaa
    49861 ctctacgtta atcaatatcg ggattttcgc tttttcttta gcattacttt tccaattggt
    49921 gacattacca gtcgaattta acgcgtctcg gcgagcaatc caaattttga gtgaaggcgg
    49981 actactgacg gaagaagaag taccgatggc aagacacgta ttatttgcag cagcactgac
    50041 ttatgttgca gcagcgcttt cgacattcct gcagctgttg cgcttgatct tattgttcgg
    50101 cggcaaccga agagattaaa ataaagaagt tgtgaaatca gcgctttgat ctgagctaat
    50161 accgagagtt agctgattga ataccattta tttggaa
//
LOCUS       DAYYBH010000014        49935 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000014 DAYYBH010000000
VERSION     DAYYBH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.014"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..125)
                     /locus_tag="ACHAUG_000937"
     CDS             complement(<1..125)
                     /locus_tag="ACHAUG_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8480049.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQ"
     gene            complement(127..324)
                     /locus_tag="ACHAUG_000938"
     CDS             complement(127..324)
                     /locus_tag="ACHAUG_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8480050.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(718..2445)
                     /locus_tag="ACHAUG_000939"
     CDS             complement(718..2445)
                     /locus_tag="ACHAUG_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8480051.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2438..3631)
                     /locus_tag="ACHAUG_000940"
     CDS             complement(2438..3631)
                     /locus_tag="ACHAUG_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8480052.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3818..4462)
                     /locus_tag="ACHAUG_000941"
     CDS             complement(3818..4462)
                     /locus_tag="ACHAUG_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8480053.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4556..4690)
                     /locus_tag="ACHAUG_000942"
     CDS             complement(4556..4690)
                     /locus_tag="ACHAUG_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8480054.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4692..6824)
                     /gene="feoB"
                     /locus_tag="ACHAUG_000943"
     CDS             complement(4692..6824)
                     /gene="feoB"
                     /locus_tag="ACHAUG_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8480055.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6821..7294)
                     /locus_tag="ACHAUG_000944"
     CDS             complement(6821..7294)
                     /locus_tag="ACHAUG_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8480056.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7695..7919
                     /gene="nrdH"
                     /locus_tag="ACHAUG_000945"
     CDS             7695..7919
                     /gene="nrdH"
                     /locus_tag="ACHAUG_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8480057.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8078..10237
                     /gene="nrdE"
                     /locus_tag="ACHAUG_000946"
     CDS             8078..10237
                     /gene="nrdE"
                     /locus_tag="ACHAUG_000946"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8480058.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10287..11252
                     /gene="nrdF"
                     /locus_tag="ACHAUG_000947"
     CDS             10287..11252
                     /gene="nrdF"
                     /locus_tag="ACHAUG_000947"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8480059.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11341..12480)
                     /locus_tag="ACHAUG_000948"
     CDS             complement(11341..12480)
                     /locus_tag="ACHAUG_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8480060.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12789..13502)
                     /locus_tag="ACHAUG_000949"
     CDS             complement(12789..13502)
                     /locus_tag="ACHAUG_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8480061.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13756..14457)
                     /gene="nagB"
                     /locus_tag="ACHAUG_000950"
     CDS             complement(13756..14457)
                     /gene="nagB"
                     /locus_tag="ACHAUG_000950"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8480062.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14691..16223
                     /locus_tag="ACHAUG_000951"
     CDS             14691..16223
                     /locus_tag="ACHAUG_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8480063.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16414..17616)
                     /locus_tag="ACHAUG_000952"
     CDS             complement(16414..17616)
                     /locus_tag="ACHAUG_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8480064.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17698..18387)
                     /locus_tag="ACHAUG_000953"
     CDS             complement(17698..18387)
                     /locus_tag="ACHAUG_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8480065.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18493..18861)
                     /gene="rplL"
                     /locus_tag="ACHAUG_000954"
     CDS             complement(18493..18861)
                     /gene="rplL"
                     /locus_tag="ACHAUG_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8480066.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18914..19417)
                     /gene="rplJ"
                     /locus_tag="ACHAUG_000955"
     CDS             complement(18914..19417)
                     /gene="rplJ"
                     /locus_tag="ACHAUG_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8480067.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19621..19748)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19827..20516)
                     /gene="rplA"
                     /locus_tag="ACHAUG_000956"
     CDS             complement(19827..20516)
                     /gene="rplA"
                     /locus_tag="ACHAUG_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8480068.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20620..21042)
                     /gene="rplK"
                     /locus_tag="ACHAUG_000957"
     CDS             complement(20620..21042)
                     /gene="rplK"
                     /locus_tag="ACHAUG_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8480069.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21385..22053
                     /gene="sdaAB"
                     /locus_tag="ACHAUG_000958"
     CDS             21385..22053
                     /gene="sdaAB"
                     /locus_tag="ACHAUG_000958"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8480070.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22066..22938
                     /gene="sdaAA"
                     /locus_tag="ACHAUG_000959"
     CDS             22066..22938
                     /gene="sdaAA"
                     /locus_tag="ACHAUG_000959"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8480071.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23029..24129)
                     /locus_tag="ACHAUG_000960"
                     /pseudo
     CDS             complement(23029..24129)
                     /locus_tag="ACHAUG_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24221..25108)
                     /locus_tag="ACHAUG_000961"
     CDS             complement(24221..25108)
                     /locus_tag="ACHAUG_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8480072.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25185..25730)
                     /gene="nusG"
                     /locus_tag="ACHAUG_000962"
     CDS             complement(25185..25730)
                     /gene="nusG"
                     /locus_tag="ACHAUG_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8480073.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25831..26001)
                     /gene="secE"
                     /locus_tag="ACHAUG_000963"
     CDS             complement(25831..26001)
                     /gene="secE"
                     /locus_tag="ACHAUG_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8480074.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26025..26177)
                     /gene="rpmG"
                     /locus_tag="ACHAUG_000964"
     CDS             complement(26025..26177)
                     /gene="rpmG"
                     /locus_tag="ACHAUG_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8480075.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26339..26983
                     /gene="cbpA"
                     /locus_tag="ACHAUG_000965"
     CDS             26339..26983
                     /gene="cbpA"
                     /locus_tag="ACHAUG_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8480076.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27039..27542)
                     /locus_tag="ACHAUG_000966"
     CDS             complement(27039..27542)
                     /locus_tag="ACHAUG_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8480077.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27555..27601)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28139..29104
                     /locus_tag="ACHAUG_000967"
     CDS             28139..29104
                     /locus_tag="ACHAUG_000967"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8480078.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29203..30375)
                     /locus_tag="ACHAUG_000968"
     CDS             complement(29203..30375)
                     /locus_tag="ACHAUG_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480079.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30362..31507)
                     /locus_tag="ACHAUG_000969"
     CDS             complement(30362..31507)
                     /locus_tag="ACHAUG_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480080.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31521..32445)
                     /locus_tag="ACHAUG_000970"
                     /pseudo
     CDS             complement(31521..32445)
                     /locus_tag="ACHAUG_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32562..33641
                     /locus_tag="ACHAUG_000971"
     CDS             32562..33641
                     /locus_tag="ACHAUG_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8480081.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33638..34266
                     /locus_tag="ACHAUG_000972"
                     /pseudo
     CDS             33638..34266
                     /locus_tag="ACHAUG_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34376..34603)
                     /locus_tag="ACHAUG_000973"
     CDS             complement(34376..34603)
                     /locus_tag="ACHAUG_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8480082.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34706..37294)
                     /locus_tag="ACHAUG_000974"
     CDS             complement(34706..37294)
                     /locus_tag="ACHAUG_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8480083.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37314..37973)
                     /locus_tag="ACHAUG_000975"
     CDS             complement(37314..37973)
                     /locus_tag="ACHAUG_000975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8480084.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38022..38525)
                     /gene="trmL"
                     /locus_tag="ACHAUG_000976"
     CDS             complement(38022..38525)
                     /gene="trmL"
                     /locus_tag="ACHAUG_000976"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8480085.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38746..39612)
                     /locus_tag="ACHAUG_000977"
     CDS             complement(38746..39612)
                     /locus_tag="ACHAUG_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8480086.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39721..40362
                     /locus_tag="ACHAUG_000978"
     CDS             39721..40362
                     /locus_tag="ACHAUG_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8480087.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40424..41785)
                     /gene="mgtE"
                     /locus_tag="ACHAUG_000979"
     CDS             complement(40424..41785)
                     /gene="mgtE"
                     /locus_tag="ACHAUG_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8480088.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41803..42702)
                     /locus_tag="ACHAUG_000980"
     CDS             complement(41803..42702)
                     /locus_tag="ACHAUG_000980"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8480089.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42704..43501)
                     /locus_tag="ACHAUG_000981"
     CDS             complement(42704..43501)
                     /locus_tag="ACHAUG_000981"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8480090.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43479..44183)
                     /locus_tag="ACHAUG_000982"
     CDS             complement(43479..44183)
                     /locus_tag="ACHAUG_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8480091.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44314..44890
                     /locus_tag="ACHAUG_000983"
                     /pseudo
     CDS             44314..44890
                     /locus_tag="ACHAUG_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45008..45685
                     /locus_tag="ACHAUG_000984"
     CDS             45008..45685
                     /locus_tag="ACHAUG_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8480092.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45764..47572)
                     /gene="pepF"
                     /locus_tag="ACHAUG_000985"
     CDS             complement(45764..47572)
                     /gene="pepF"
                     /locus_tag="ACHAUG_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8480093.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47748..48746)
                     /locus_tag="ACHAUG_000986"
     CDS             complement(47748..48746)
                     /locus_tag="ACHAUG_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8480094.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48972..49625)
                     /locus_tag="ACHAUG_000987"
     CDS             complement(48972..49625)
                     /locus_tag="ACHAUG_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8480095.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14946 a        10480 c         8765 g        15744 t
ORIGIN      
        1 ttttgttttt ctaattttaa atataccttt tgtaaaacct caagaatatc agaatattta
       61 ggatctttac ttatagatat atcattatat aattcatgaa caatttgctt agcatttttt
      121 ttcattttag cacttccctg gaattgctcc acctaaaaaa ccacctatag acattcctgc
      181 aatacaagta gttgccttgg cccaatcgac cgtacattta tttttagtgc aatacactcc
      241 atttccataa tattttccac tatgagtggt acccccatag ataagttgtg tctctttaat
      301 agacaaaatt tttaaatgtt tcataatcta atcaccccta aattaatgtt tttagactat
      361 tattctaaca ttcattaaat ttcaaaaaac aattccttct tgaaacgtag caaaataatt
      421 cctgaataag aatttaatca cttttaagta tataaactat gtatattctt taatattttt
      481 tgagaatgca cttcgtacat aataaacttt aatcacatta gtctttcata cctacgatcg
      541 atctctttga ctcgcaccca taaattcata atagtttgtc ggcgtctttg gacgtttcga
      601 agatgagcta cgcattctct ttttttatag acaaaaaaga tagagacgaa catggagctt
      661 ccaaatttgg atatttgatc caattttgga agctccattt atttcgtgtc tgtcttttta
      721 ttttttccag ctttcgacta attcttggtt ctctttgatc cagtcttttg ctgcttgctg
      781 tggatctttt ccattgttga tttccaacat aactttttcc atatcttttt ctgaccaatg
      841 gaaattgtct aatactttgt aagcttctgg ctggtcttct ttcaatcctt ttcgaaccat
      901 cgtatggatc tgctcttcgc ttcccattcc gttttcagga tcttctaagt atttcagatc
      961 atatttggca aacatccaat gaggtgtcca tcctgtaact acaattggtt cgtgtttttt
     1021 gatcgcttga cctagtgcaa cggtcattgc tcctgaagaa gatgtctcta ctttccagtc
     1081 tttcaagttg tcatattttt ggatcgtgtt ttcagcagcc gtaactacac cagctcctgg
     1141 ctcgattcct atgattttct tacctgcctg atcggttaac tcatcgatcg aatttacatc
     1201 catgtaggct ggcactgcta aaccaacttt tgctcctgtc aaattggcac ctaggtcttc
     1261 gacttgatct ttatattgtg catattgtga accatgagtt ttaggtagcc aagctgaaac
     1321 catggcatct gactcgccat ttgaaacaga tttccacatg attgaattat ctaatggcgt
     1381 catcgttaca tcatagccca tttgtttcaa tacttcaccg actacatttg ttgaagcaac
     1441 ttctgtatcc cattcaacat aagataagtt gatacgtttt gtttctttgg cagatgaaaa
     1501 actccctata ccaatcgcac ctaagatcac tactactaca gcaccaatga tcagtccttg
     1561 acgtttttta cttttctgtg ttgttgctcc tgcagctttt ttattgtttg gctggttcaa
     1621 atgctgtgta aaacggtcaa tgatgattgc taagataact aatgcaaccc cgttgacaaa
     1681 tccattcccg acttgtgctc tttgcaatgc tgataatacg ccgcgtccta agccgggtgc
     1741 accgatcata gaggcaataa cgaccattga aagtgacagc attgttgttt gattgatccc
     1801 tgccatgatc gtacttttag ctaaaggcaa ttctagtttg aatagttttt gacgacttgt
     1861 gctcccgaat gaatcagaag cttctactaa ttctttagga acttgtcgaa tccctaaatt
     1921 cgtaaaacga acggttggtg gcagagcaaa gatgactgag gcaaataccc ctggaaccat
     1981 cccgataccg aaaaatgcaa ctgctggaat caaataaacg aatcctggca ttgtctgcat
     2041 aaagtcaagg atcggtttga taatactttg ggctttactg ctttttgcca tcaagatccc
     2101 taatggtacc ccaatgatga tcgagatcac actagataaa agaacaagtg tgacagtatt
     2161 catcaaatca ttccatagac cttggttata aataaaccat agaccgatta atgtgaacaa
     2221 cgtaagtcct ggacgtttct tagaaataaa gaaagctgct accgtcaata acaagataaa
     2281 caaaggagct ggaatcacta acagtaaatt tgtaatcccc gacatcactg cctgtccaat
     2341 cgtttgtaaa aatgaaaata ggccagaaaa agttgtcgta aaccattctg tgatattttc
     2401 tacccagttt gccactggta attgatagtt caatagatta ttcatgttca ctcacctcgc
     2461 ttgtttctgc taatgcttca atcacactac ctcggatgac gacacctttt aattttccat
     2521 cctctacaac tgcaagtgga gtcggtgagt cataaataat attgaaaata tcacttacca
     2581 ccatgtcttt cccaatcacc gtcacatttt catctaggaa gtcgatcaat ggacgttttt
     2641 gcttcctagc ttctaacgct tgatctgcag tgatgatccc tttcaggtgt ctctttctgt
     2701 caacagccat cagcatgctg acttcttcat tacgcatcct tgtcaatgca acgtttggtc
     2761 catcagattc gatattcgtc gtcaatgcag gtaccatgat gttttgagcc gtaagcactt
     2821 tggaacgatc gacttcttcc acaaattcac gtacataatc atttgctgga ttggtcaata
     2881 tttcttcacc tgttccgatc tgcatgattt cgccgtcttt catcaaggcg attctgtccc
     2941 caatccgtaa agcttcattc aaatcatggg taatgaaaat aatcgttttt tgaacattgg
     3001 cttgcaggtc cagtaattcg tcttgcattt ctcgccggat cagcggatca agagccgaga
     3061 acgcttcgtc catcagcaag atctcaggat catttgctaa agctctggct aggccaaccc
     3121 gttgttgcat cccaccagat aactgactag gatattggtc tttaaatgaa agcaaactag
     3181 aattttctaa tgctttttct gctttttctt gtcgttcttc tttaggtaca ccacggactt
     3241 ctaacccgta ttctgtattt tccaagatcg ttcgatgtgg aaacagaccg aagttttgga
     3301 aaaccatatt gattttatga cgacggactt ctcgcaattc ttctttgctt aacttcgata
     3361 catcttctcc atcaatatag atactgcccg aagttggctc aatcagacga ttcaatagac
     3421 ggatcaatgt ggatttccca ctcccggaaa gccccatgat cacgaagatt tctccttctt
     3481 ttacgtcaaa attcacgtca tacacaccaa ccgttgcacc ggttttcttt aaaatctccg
     3541 ttttcggctg gtgatcattc atcatatcta aagcggcttg tgtcttttta ccaaatatct
     3601 ttgttaaatg ttttacttct acttttacca aacaaattct ccttttttat gtaaattcat
     3661 ttttctttct gaaaaaagtg accacactat gttaacatca tgtagtcact ttgtcaaaaa
     3721 tgaaagaaat ttgcttgtcc aaactgaaaa aaacttggct aaatctttgt aaataaaagg
     3781 tttagccaag ttaatatttt tttaatattt cacacaatca ttctgcaagc tgatgctccg
     3841 ggtaaaaaaa gtttgtcata cgctgtagtg tatattcatt tccaataaaa tgaacgatgt
     3901 cccctgccgt aaatttagca taggggccag gtgaaacaag cagttcttca ttatgagaaa
     3961 ttgcgataac tgtggcgcca gtcgcctgcc agatattcaa ctcgctaatt gaacggtcca
     4021 gatgcattgc tttttctgat aaagataatt caaacggaag taaaggattc atcttatgta
     4081 ttcgcttcgt ttgattgacc agctgatcca ataattgaga aaaatgattc agctcatctt
     4141 gttgcttctc cacacttgcc atgatttctg cttttgtatc ttggatggat tgtacatctt
     4201 gatattgcaa taggaaatct tttgcttttt ccttcgaagc aacaaatgcc ccacttccat
     4261 gccgtacttc catgattcca agatctacca gcacattgat agccttgcgg gtagtctcag
     4321 gagaagcatt gtacgtactg gcaagtgttg agcgggcatg gatcttctca cccactttat
     4381 atcggttttc aacgatacgt tcggctaaat caacagcaat ctgctggtag cgcgggcgga
     4441 cgattttttt ctttccagac attttctttc ctctttcttt ttttactttc cctcatctta
     4501 ctatgctcta gcaaaataag caaaaaaggg gagaatagcc tcttgcaacg actgatcatc
     4561 gcttgatctg ttcctttttt actgggcaag ctgtttgaca gtcatcacaa ttcttaccgg
     4621 attttaaacg agtatagaca attgcgcctg cacttccaaa gattaacaca cttaataaga
     4681 atgtcgccat cctattcctc tccttctaat gacaatatag tataaaactg gttcttggat
     4741 tttggctgac ggaacaaaac ataaacgagt cctatcatca gtaaaatagc aagatacgtc
     4801 cctaacgcta cctgtccacc ctccaataag tgtcccaact gataaatcac aaaactaact
     4861 aaataagcta aaccgcattg atagcctatg gcgatccaag tccatttctt ggcattcatt
     4921 tcccgatgga tagccccaat tgccgcaaaa caaggcgcac aaagtaaatt gaaggctaac
     4981 aatgaataag ctgctgcggg agtatatgtg tgttgtagca cttgccagat ttctctgcca
     5041 ttttcggata cttccgcatg accaaacaaa ataccaaatg tgccaataat attttcttta
     5101 gcgaccaatc ctgtaatcgc tgcgactgtt ccttgccagt ttccccaacc aagcggctga
     5161 aaaacaactg ccaggattcg cccgaaaaag gctaagatac tttggtcttc agaaacaggt
     5221 tgagcataaa aattataaga agagctgaac cagatcacga tactagaaac aaagataatc
     5281 gttcctgctt ttttaacaaa agacttgctg cggtcatagg tttgtcttag gaccgctgac
     5341 cattgaggaa aatgatatgc gggcaattcc atgataaaag gagctgtatc tccagcaaac
     5401 aaacgtgttt ttttcaagct gattccagag aaaataattg cagtcatacc aagaaaatag
     5461 gcagaaggag ctacccaaga ttgatgagga aaaaaggccc ctgctactaa ggcaataatc
     5521 ggcaactttg cagaacaagg catgaaggtt gtgaccatga tcgtcaaacg acgatcttgc
     5581 tcgttttcaa tcgtgcgact ggccatgacg cctggcactc cacatcctgt agcaatcagc
     5641 ataggaatga aggattttcc tgaaagaccg aatttcctga aaagtcgatc catgacaaaa
     5701 gcgatcctcg ccatatatcc gcagtcttcc agaaagctca agcataaaaa caatataatc
     5761 aattgcggta gaaatcctaa cactgctccc acgcctgcga tgatgccgtt caagatcagc
     5821 tcctgcatcc atcccgctac ttgccattca gctaacaatc tgccaacatg ttctggtaac
     5881 catgtaccaa acaatgtgtc attgatccaa tctgtcccca ttgttcctac tgtttgaata
     5941 gccagataat agatcagcca catcacaaat gcgaagatcg gcaaggctaa ccaacgattc
     6001 gttgcaatct gatcgatttt gtcactcatc gataattgga aagaatcatt tgacacaaca
     6061 cagagtgtac atagttgggt aataaattca taacgttcat tgaccagaat cgtctcacta
     6121 tcgtcgtcca atagtttctc tgttaactgg atcagttgct cgatctcttt ttgctgagaa
     6181 actgctatct cgtaatcctc tcttaccttc tgatctcgtt caaaatattt cattgcatac
     6241 cagcgtttct tgccgctttg ttctcctagt acatcttcaa tttcatgtaa agctgcttct
     6301 aaacgtggat catatgtaca gggaacagga atagaaggcc gcttttttgc agactcctga
     6361 atcttttcga ttagtttttc gattccttgc tttttcaata cgctgattgg taaaactggt
     6421 acaccaagac cataggaaag cttttctaga ttgatttttt ttccttcttt tttgatgaca
     6481 tccatcatat tcagaccaat gatcatagga atgtctaaat ccaataactg cgtagttaaa
     6541 taaagatttc gttctaaatt ggtagcatca ataatgttga tgatcagttc aggctgttcc
     6601 tgaaccaaat aatctcttgc aatcacttct tctggtgtat aaggagacaa agaatagatg
     6661 ccgggtaggt cctgaatatg aatgttcgga ttttttttca acgatccttc ttttttttct
     6721 actgttacgc ccggccaatt ccctacatat tgattcgaac cagtcaagag attgaacaag
     6781 ctagttttcc cgctgtttgg atttccgact aacgcgatat tcattctttt tcctcccttt
     6841 gtatcaagat tagttcggcc tctgactttc gtaacgtcag ttcataccct cgcactgtca
     6901 gttcgattgg atctcccaga ggggctagtt ttcggataac tatttttaca tttcgcgtca
     6961 atcccatatc cattaaccgt cgcttcactg cgccttgtcc gtggattgct acgacgatcc
     7021 cagtctctcc tgcttttaat tgatcaagag aagtccagct ttcagaggaa gcagtctttt
     7081 tattgacaaa gatttgacct agtatttccc gacttactgc cactcgatga ctttttacca
     7141 gtaaaatggc attgttgcct gataaattga cgacgacgat tttacttccg gcaacgatcc
     7201 cgatattttg caaatggcgt tttgtttttt cttctgtttc gattcgttcg acttgataaa
     7261 cgaaattctg ctttgcctgc gctaaggtct gcatattgat tcctctcttg tgaaataaat
     7321 catgtattaa aatcattcat tatatgttct aattttctta cgtgttacgc aaataaatga
     7381 gaatcattct cacatagaat actctcggta attgagaatg tcaattatac ttgtgatatt
     7441 atgagcgaat ctttagtatt gatagataaa aatgagaaaa aaatatttct tcatagtttt
     7501 ccacaatttt ttctctatta aatttgctat tttcccacac gatacttgtt ttcagctctt
     7561 ttttcaaaaa cacaaaactt agtattccac agtttccaca acacaatatg tagtgtttgt
     7621 tgtttgactt ttttactaag agttttatac tatctattat agataataag aatgtgttag
     7681 aggaggcact caccatgaac gtaaaaatct tttctaaaaa taattgtatg caatgtaaga
     7741 tggtcaaacg gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat atcgatgaac
     7801 agcctgaagc agtagactgg ttgaaagcac aaggattcca aagtgttcct gtcattactt
     7861 ctgatgcaga aacagttatc ggattccgtc ctgatcagtt gcgtaaactt gccagctaac
     7921 atgactaaaa gaatcaaaac gtgatacatg gatttgaagg gcttgagaca gcaccagttt
     7981 ttgtcgcaaa cttccaaatc cttttttaaa gcttaaaaac aatgcaatga tttgcaatca
     8041 agcatcatca aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa atttacaaga
     8101 tgtcagttat ttcaagctga ataatgaaat caatcggcca gtcaatggtc aaatcccttt
     8161 gaataaggat aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac caaatacgat
     8221 gacttttcta tctattatgg aaaaaatcaa ttacttgatc gatcaagaat atatagaagc
     8281 agaattcatt caattgtatt caccatcatt tattgaaaag ctatatcaat ttttggacga
     8341 gcaacatttt tctttcaaat cttttatggc tgcttacaaa ttttattctc aatatgcgtt
     8401 gaaaaccaac gacggttccg cttatctgga aacttatgaa gatcgagtag ccttcaatgc
     8461 gctttacttt gcgaacgggg atgaagaatt agcacttgct cttgcagacg aaatgatcca
     8521 tcaacgatat cagccagcta caccttcttt cttgaatgct ggacgtaaac gtcggggcga
     8581 attggtgtct tgtttcttga ctcaagtcac tgacgatatg aactctatcg ggcgctcaat
     8641 caactctgct ttgcaattgt cccgtattgg tggcggcgta gggattactt tatcaaatct
     8701 tcgtgaagcc ggcgctccta tcaaaggata tgagggtgct gcaagtggtg tcgttccagt
     8761 catgaaatta ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc gtcaaggagc
     8821 tggcgtggtt tatttgaacg ttttccatcc agacattatt gctttccttt cagcaaaaaa
     8881 agaaaatgca gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga tcgtacctga
     8941 taaattttat gaattgacac gtaaaaacga agatatgtat ttatttagtc catatagtgt
     9001 cgaaaaagaa tatggtgtcc cattctctta tgtaaatatt acagctgagt atgatcatct
     9061 agttgcaaat ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg aaaatgaaat
     9121 ctcaaaatta caacaagaat ctggctatcc ttacgtaatc aatattgata cagcgaaccg
     9181 cgagaatcca atcgatggca agatcatcat gagtaattta tgttcagaga ttttacaagt
     9241 tcaaacgcct tctgtgatta acggcaaaca ggaatatgaa gtattaggaa cggatatctc
     9301 atgtaactta ggctcgacaa atatcgtgaa tttgatgcaa agccctaact ttggtcaatc
     9361 tgtgcgtacg atgactcgtg cattgacatt tattacagat gcatctgata tcgatgtcgt
     9421 accaccgatc caaaacggaa ataagctgaa tcatacgatt ggattaggtg ccatgggctt
     9481 acacacttat cttgcaaaag aacaaatgga atatggctca gaggattcat tagactttac
     9541 aaatatttac ttcatgctgt tgaattattg gacacttgtt gaaagcaaca atatcgctcg
     9601 tgaaagaaaa caagttttcc ataattttga aaaatcagct tatgctgacg gcacttattt
     9661 cgaaaaatac gtaactggag aattccagcc aaaatcagaa aaagtcaaag cattgtttga
     9721 aggcattttt atcccaactg ctgaagattg gaatgctttg aaacaagcag taatgaaaga
     9781 tggactgtat catcaaaatc gcttggcagt agcaccaaat ggctcaattt catatatcaa
     9841 cgatacgagt gcaagtattc atcctatcac tcgcatgatc gaagaacgcc aagagaagaa
     9901 aatcggtaaa atttattacc cagcgcctta tctatccaat gagacgattc cgtattacac
     9961 ttctgcttat gatatggaca tgcgtaaagt aatcgatgtt tatgcaatgg cacaacaaca
    10021 cgtcgatcaa ggcatgagtt tgactttatt catgcgttct gaaatcccag aaggcattta
    10081 tgaatggaag acaacaacga agcaaacgac acgtgatttg aatattttaa gacattacgc
    10141 tttccacaaa ggaatcaaat ccatttatta cgtacgtacc tttactgatg atgcagaaga
    10201 aatcggaagc aatcaatgtg aaagctgcgt gatctaatat catagataga ttcgcttatt
    10261 catattttaa ttaaaggagc attctcatgt cagaaacata ttatgaagca ataaactgga
    10321 atgaaattga agatatcatt gataaatcca cttgggagaa gctgacagag caattttggc
    10381 tagatacacg tatcccactt tcaaatgacc tagatgattg gcgtacattg tctcaattgg
    10441 aaaaagatac agtaggacat gtatttggcg gtttgacact gctagatact gtccaatctg
    10501 aaagtggcat ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa gccgttttga
    10561 acaatattca attcatggaa tccgttcatg ccaaaagtta ttcttctatt tttagtacac
    10621 tgaatacgaa aaaagaaatc gaagaaattt ttgactggac aaatacgaat ccttatctgc
    10681 aaaaaaaagc tgagcgtatc aatgaaatct acaaacacgg tacaccgcta gaaaagaaaa
    10741 ttgccagcgt ctttttagaa acattcttgt tctattcagg tttttatact cctctttatt
    10801 atttaggaaa caacaaattg gctaacgtag cagagatcat caaactaatc atccgagatg
    10861 aatctgtaca tggaacctac atcggttaca aattccaatt aggattcaac gagttacctg
    10921 aagcagaaca agaaacgtta aaagactgga tgtacaatct tctttatgag ctttacgaaa
    10981 acgaagaacg ttatacagaa gaattatacg atccaatcgg ctggacagaa gaagtcaaga
    11041 cattcctacg ctataatgcc aacaaagcat tgatgaacct agggatggat ccactctttc
    11101 cagatactgc caatgatgtc aacccaatcg taatgaacgg gatttcaaca ggcacaagca
    11161 accatgactt cttctcacaa gtcggaaacg gctatttgtt aggtcatgta gaagcgatga
    11221 aagacgacga ttatttgatt ggattggaat aaggatttat tttaattcaa aaatttttcg
    11281 aaaaataaaa agagtttagc gtacagatat tttaaatgta cactgaactc ttttttatgc
    11341 ttattcactt gtttcttcca gcacttttgg actgtctaac ttttccgaaa catgttccat
    11401 tcttctcaat aaatagattt tccggatatt taatattatc gtcttaatca ctgcatagac
    11461 aggtatgcaa atcagcatgc ctaatattcc tgcgacactt cctgctccaa tcaataccaa
    11521 gatgatcgtc aaaggatgga tattcattga tttcccaaat aacaacggct tgatcaaatt
    11581 tccatctaat tgttggatca ctaaaatcga caacgccata tacaaagctt gaaatggaga
    11641 gataaacaaa ccgacaatga ttgctggtac cgctccaata aaagggccga catacgggat
    11701 aatattcgtg attccgcaaa tgaccgcaag caacaagcta taaggctgat cgaatattct
    11761 cattgccaaa aagctcatta caccaataat caaagcatct aagatcgtac tgctgatata
    11821 ggcagataat gtttcgttta attcttttac cgtttggcgt aactctttac gaatcgcttt
    11881 cggaaagaag tgagacaacg catcaataaa tttgtgccca tccttgaaca taaatagcaa
    11941 aataaatgga acggtaaata acaaaataaa aaattgcatc ataaatgaaa tgatttttga
    12001 gacacttgca gataaactga ctaatatgat attgaacaaa ttactaagag ataaatttgc
    12061 tgatgctaat tgttcttcca aattgaaatt tttaaactct tcccgctgcg ccaattcatt
    12121 taaccatttg cttgtttcat ctgcatacat tggcaagctc tgggtcaatt gaattgtctg
    12181 ttccattaac tgaggcacta cattcatgac agttaaaaca actaaaatga ttacgaccaa
    12241 aaacgtcaat aaaaaaccga acactctcgg gatccgatgc ttttcgagaa aaacaacgat
    12301 tggatcgaac atataataga taaaaccagc aacaagcaat ggtaaaaaaa ctgccgaaag
    12361 cattttcaat attggactaa aaatattcgg catttgtaaa ataaagtaca cgccgatgat
    12421 cgtgagtact agctccactg tccagaaaaa taattttgat tgtttaaaac gtgattgcat
    12481 acatctcctc caaggtcctt tgatacttct attctatcat ttaacaaaga gttacgcgct
    12541 gagattttca tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg agtagacgag
    12601 tacttaaaca aaaacacggt ttggagtagt gacaaaaatt atactgcagt ccctttttcc
    12661 aaataaacgg tgttcaaaag cttgcatctt cgtaataagc ccagccaaac gaaaaatata
    12721 aggaactatt tttatttggc tgggcttatt acttgatgta ggacatctat tttacaacct
    12781 cagatcattt attgcgcaat actgactaaa tgcaactttg tgatattttt gtgtacggac
    12841 aactcctcga tcacatcggc ataagtgagc ccttttggaa gatcaatcgt atagatattt
    12901 ttgtacactc ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc gattacgata
    12961 tttttctctt caaaataatc ggtaagaaaa tctttggttt cttttctatg gataaattgg
    13021 atttctaact ttttagtcgt aggtacatgg atcacgcgtt tcactactgt gagtgcaata
    13081 gcaatcccga taaaactgat gatcgcaatg gaatagtaac ccatgccgat ggcgatccct
    13141 aatccagcaa ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga ttgtttcgtg
    13201 acgatgatcg ttcccgcgcc taagaaaccg acaccactca ccacttgagc aatcagcctt
    13261 gcttcatctg aacgaatcgt tccagcaagc tgtggatact ctaaagcatc tcttaatgca
    13321 ccactagcga tctcaacttg gatcaatgca atgatacatg ctcccataca aactaaaata
    13381 tgcgtacgca tacctgctgg acgattttta tattgtcgtt caaaaccgat tgctccgcca
    13441 aatagcatag cgatccctaa acgtaaaata atctctggaa ttgtcaatac catatcattc
    13501 atctaaacac cctccagtcc aatatatcat gtctgaaagg agcagacaaa aaaagtgcaa
    13561 tttttacctc ttacgaaaaa aggactgtga caaaattttt gtcatagtcc tttttatgaa
    13621 taaacggtgt tcaatcagct gaccctcggt attactcaac aaacggcaaa atatggggag
    13681 ctatttttcc tttgtcttct ctaatactca ggtcaaaacg ctgatttcac aacctcttta
    13741 gcctactatt gaaaattaca acttacttgc tgcttcttca tcgatgatca caacaacatt
    13801 gtcgtgattt tgcaatacac ttgctggcaa atgattcgtt acaggtcctt caatcatccc
    13861 tttgattgca tcagctttgc tttcgccaaa tgccatcaag acgatttctt tacttttcat
    13921 aattgaaccg attcccattg aaacagcttt tgttggtaca tcttctgctt tatcgaagaa
    13981 tcgtttgttt gcattgattg ttgattctgt taagtcaact actgaagtcg ttccgtcaaa
    14041 tgaagcacca ggttcattga aaccgatatg tccattttgc ccgattccta agatttggat
    14101 atcgattgga tgtgcatcga tgatttgatc atactttttg catgctgctt ccagatcttc
    14161 tgcttttcca tctggtacaa acgtttcttt gaatggtttt ttatcaaata attggacatc
    14221 catgaaatga cggtagcttt gttcatctga accgcctagt ccaacgtact catctaagtt
    14281 tacagaagtc atttcactga aatccaaatc gcttgaagtc atttctttat ataaagtaac
    14341 tggtgtgctt cctgttgcca atcctaatac ttttgcacct ttgttcatac cttctttgat
    14401 gatttcaaat gctttttttc cgccttcttc agcgtttttt acacgaatga tttccatagt
    14461 gattgctcct ttatattttg gtatagccca attatagcat tctttttttc tttttacaat
    14521 aattagtata gaccaaattg catttttttc attttcttga caataaatag gaatccgtgt
    14581 taggtttcat aagatggttc aatcaaacaa tccatgttgc atgaaaacca agcaagtgat
    14641 tcactgtgga cgctttatgt ctgcagtttt tttatgaaaa aggagttatt atgagtatac
    14701 tgacattaaa caacattacc cagcaatttg gcgacaaaat cctctatgaa ggtgtcgatc
    14761 tgcaattaaa tgctggcgaa catcttgggc tgattggaca aaacggagcc ggtaaaagta
    14821 cattgattaa aatcatcact ggtgagatct taccagatga cggacagatc cattggcaaa
    14881 aaaatattca caaaggttac ctcgatcaat atgtggaagt cgatgaaaca ttaacaatcg
    14941 aagaatttct aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa attgcttttc
    15001 tttaccaaac atatagcgag accttcgatg attccttgct ggaaaaagct gggaaactcc
    15061 aaacggaatt agaccaagga gtgttttatc aaatcgacac cttagttgct gagatgagca
    15121 gcgggttggg gatcgatgtc ttaggtttag attcactgtt gaaaaatcta agtggtggac
    15181 aacgttcaaa ggtgatttta gcaaaactac ttttagaaaa cccagatgtt ctgatcttag
    15241 atgaaccaac caatcactta gatgatcagc atatccaatg gctgacgcag tttcttcagg
    15301 attttgacgg tgcgtatatc gtgatttccc atgaccaaga atttcttgat cagatcacca
    15361 cccatatcgc agacatcgag tttggaaaga ttactaagta tactggccat ctaaaacaag
    15421 cattgaaaca aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat gctcaacaaa
    15481 aacaaatcga aaagaccgaa gcttatattc gaaaatacaa agccggttcc cgatcaacga
    15541 tggcaaaaag ccgacaaaaa caattagata aaatcgaacg gctgacgcct ccagcttcag
    15601 caagtaaagc tgtctttgat tttccattct cgcctattgt cacgacatta gcagtggaaa
    15661 caactgattt agtcatcgga tatgatcgac cgctacttgc acccatcaat ttgaccatcc
    15721 gctttggtga aaatattgcc attcgaggct tcaacgggat tggaaaatct actttattaa
    15781 aaacactgat cggtgagatt gaaaaaataa gtggcgattt ccacttccca gataacacaa
    15841 aaatcaatta cttttcacaa gatctgtact gggaaaatcc attagaaaca ccactacaat
    15901 atttaagcag tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa ctagctaaag
    15961 cagggttagt taatcaatta gcaagtgaac ctctcactac attaagtggt ggggaacaaa
    16021 ctaaggtgaa acttgcacag ttgacaatga accaaggtaa tttactgatt ctcgatgaac
    16081 cgacgaacca tatcgatcaa gaaacgaaag aaagcttaca agacgggata cagaaatttc
    16141 caggaacagt aatcatcgtc tctcatgaac aagaatttta tcaagatctt gtgcaacgag
    16201 tcattgagat tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag gagctgtgac
    16261 aaaagttttg tcacaactcc ttcttctgaa taaatggtgg ccaattagct gaccttcggt
    16321 attagctcaa aaaacggcaa aatatgagga gctattttta tttggctatt cttattactt
    16381 gatgcagaac agcttttcac aacctctttt cgtctatttt ttattttcga gatttctctt
    16441 tcctttaaaa acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa gcccgctaac
    16501 cgttacacct aaccagccat actgctgcca cattgtcgtc cctagcaatg aaccagccgc
    16561 acctccaata aagtacataa acataaatac agtattattt cggttgcttg ctgcttcacc
    16621 tagcccctgt actcgtgttt gattggcgac ttgaccaaat tgattcccga cgtctaataa
    16681 aataatagct aaaagcaaaa tggccaaatg gcttccgcca accaataaag caataaagct
    16741 tattgtttgc ataacaagtc ccatatacac gatttttctt tcagaataag aatctgccag
    16801 tcgtcccacg ataggtgcag caactgctcc agacaaacca aaaatcgcta atatccctgc
    16861 ttcgaatgtt ccccaattgt aggcgggact gctgatataa aaaatcaacg tagaccaaaa
    16921 gatcgaaaat gttccaaaca tgaaaaatcc actgactgcc gcttctctta atagacgttg
    16981 agaagcaact aaatgcggca aacttttaag tgaatcaagg tatgaaacag ctgtactact
    17041 ttgctggaca gaacgcggca ttttccagta aagcagaagt gtaagaattc cgactgcaca
    17101 taaagcaaat aaatagatca tccgccaaga aaaaagactg gcaacaatac cagataccgt
    17161 ccgagaaagc aaaatcccca cgagtaaacc gctaagcaat gtacccatga ccttccctct
    17221 tgcttttggt cccgctagta ccgcaccgta aggaatgatg atttgcggca cgatcgaaag
    17281 taatcctatt aaaaaagaag cgcatgcaaa taacgtaaac gaaggcgcaa aaaaagcggc
    17341 caacagagag acggcagata acgctgccat tcgaatgatc aattttggac gatccaccac
    17401 gtcccctaaa ggaactaaaa acagtaaccc taacgcatag cctaattggg tcaacatcgt
    17461 caatatcccg actgcacttg tgctaacaga taaactagct gctacttttg ttccaatcgg
    17521 ctggatgtaa tacatattgg caacaacaac gccacaagtg attgataata gtaaaataag
    17581 cgaagttgtt aaaggttttt tttcattact cttcattgat ttcccccatt tcctatctta
    17641 gtttacaact tatggaaaag attggaaata gaaaaataca actattcgcc acacttatta
    17701 tttgtgcttt tgttttcctt gttttttctt gcgatcttta taaatgaaga caactaaccc
    17761 tactagcaca gtaacacttg caaccaacgt gactgcagtt ttgttatatc cgtgaaacag
    17821 agcttctcca ccacacgcat acaacacaga cgaagggatg actccaatca cgatcgacaa
    17881 tatgatttgc tgtagaggca atttcatcgc atctgcagca aaattgacca tagaagatgg
    17941 aataaccggt atcatatagc ctaacattac acctattttt ggatgtttca tttgtcggat
    18001 tgcttgcacc caatgattcg tttcggtttt cttatctaaa aattttaagc gatgcataag
    18061 gaaaattgca gtgatgttac caagagcatt acccaaaatg ttgatgatcg cacctgtcag
    18121 tggtccaaag ctcaaaccag caatgactcc aactactgaa gtaggaatac ctggaacagc
    18181 acacatcacc gctgtcaata cgatcaagag tatcgatgca aaagtcccat gcgaacgaat
    18241 atgtgcgagt agctctgcct tactcgctct cggatttaaa aataattgga tgtctgtctg
    18301 atattctaaa tacaaccgat agcccactac cagtatcaga atgattccca acgagatcaa
    18361 tatcaatcgg attattttct ttttcatatt tcctcctgaa aaaaatctat attcattaaa
    18421 taatagttta acataaaaac aaaaaagcac cgaaatccaa cataggatct cagtgctttt
    18481 aaatagtaaa aattatttaa cagttacaga tgcgccaact tcttcaagtt tagctttcaa
    18541 ttcttcagct tcttctttag atacgccttc tttaactggt gcaggagcgc catcaactac
    18601 agctttagct tctttcaagc ctaaaccagt tgcttcacga actgctttga taactttaac
    18661 tttttggtct ccagctgcag ttaattctac agtgaattct gtttgttctt cagcagcagc
    18721 agcgccacca gcagcagcta cagctacagg agcagcagca gatacgccaa attcttcttc
    18781 aatagcttta actaggtcgt taagttctag gatagttgct tcttttaact cagcaacgat
    18841 gttttcaatg ttcaatgcca ttataatttc ctccgtttaa ttgttctttt ttatttaatg
    18901 ctttatgcgg caattaagct gcatctccat cttctttgtc tgccactgct ttgacagcgt
    18961 aagccacgtt gcggactggc gcttgtagta cagatagcaa catagataga agtccttcgc
    19021 gatttggcaa ttttgccaat gctgtgattt cttccaatga agagacttta ccttcgatga
    19081 taccgccttt gatttctaat gcttttgctt ctttagcaaa ttcgtccatg attttcgctg
    19141 gtgcgactac atcttcgtta ctgaatgcaa cagctgtagg tccagtgaat acttcgtcca
    19201 tgccttcaag gccagctttt ttagctgcac gtgaaagaat agaatttttg ataactttca
    19261 tttctacgtt agcatcacga agttgtttac gtaaacgtgt tacttcatca actgttaatc
    19321 cgcggtagtc aacgactact actgatgcag ctgctgtaaa tttttcgctt acttcatcaa
    19381 cgattgctgc tttttttgcg ataattgctt cactcatttt tgagtttcac ctcctgaatt
    19441 ttgatggaag agttttttgc actgaaaagg caagtcactt ttatagttac tttgctcaac
    19501 cagtattatt catcacctat tcacttcgtt catagtgatt ctactcgctt tgctcgaaag
    19561 gaaactcctt tcgctttgct caaccagtat tgttcatcat ctcttcactt cgttcatagt
    19621 gattcacaat aaaaaactct atgccaccgc agacatagag ggactattga atatattcaa
    19681 taaacgtcct cggtaggaaa ttaaggcttg atgccaccta ctgtcttcgg tacaagttaa
    19741 ttattaacct caactagctt agcatgctaa actagttgag tcaagaatta tttttcagtt
    19801 ggatcaatca acattctaat atttaattag aaagaagctt ggtcaacatg gataccaggt
    19861 ccaaatgttg ttgtgatcgt tacatttttc atgtattgac ctttagctgc tgatggttta
    19921 gcttttagca atacatcatg gattgctgca aagttttcaa ctaatttttc atcgtcaaat
    19981 gaaacttttc cgattggtac atggatattt ccagctttgt ccacacggta agttacttta
    20041 ccagctttca cttcgtttac tgcttttgtt acgtccattg taactgtacc agttttaggg
    20101 tttggcatta agccttttgg tcctaatact cgtcctagac gacctacagt agccatcata
    20161 tcaggtgttg caacaactac atcaaagtca aaccatccgt tttggatttt ttgaaccatg
    20221 tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt cttttgcttt gtctccttta
    20281 gcaaaaacta aaacagtttg tgttttacca gttccgtttg gtaatacaac agcaccacgg
    20341 atttgttggt ctgctttttt agggtctacg ttcaaacggt atgcaacttc aactgttgca
    20401 tcaaattttg caatgtttgt ttcttttgct aaagctactg cttcggcaac aggataaact
    20461 ttacttgaat caactttttt taatgcatct tgcatttttt tgctcttttt agccattttg
    20521 gttcctcctt gattgtggtt ataacggttg tacctcccac gtgttctata tctttcaata
    20581 tagagccaac tgagaagctg ctgtcttagg gtgtatagat tattctacag tgatccccat
    20641 gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca acgttagctg cgtttaggtc
    20701 ttccattttt aattcagcga tttctcttac ttgatcgcta gaaactttcg caactttatt
    20761 tttgtttggt tcgcctgaac ctttttcgat tttagctgct tttttcaata gaacagcagc
    20821 tggcggtgtt tttgtaacga atgtgaatga acggtcttcg tatacagaaa taacaaccgg
    20881 aatgatcaaa cctgcttgat cagctgtacg agcgttgaat tcttttgtga atcccatgat
    20941 attgataccc gcttgaccta gtgcaggacc tactggtgga gctggagttg ctttacctgc
    21001 aggaatttgc aatttaacga ttttttctac tttctttgcc acgagacata cctccttaag
    21061 tccgtgatgt ggttaattgg agatgcatat ttctcctccc acgttttatc tgatctgaaa
    21121 aaatccagac aaataatgtg caaaacaatg cacactggta cagtatagca aatttatttg
    21181 ttttttcaag atttttttct gctattttta cattttgctt gaataaacgc taaatttatt
    21241 ctaaaaaaac aacaattttt cgttctcatt tcttctttta tgttacactt gcgctagcat
    21301 ggttcaacag atgaaagcaa acaataacac ggttttttag acccttgtac aaagattcct
    21361 acataacaaa atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt ctttgatatt
    21421 attggacctg tgatgattgg tccaagtagc tctcacactg ccggagctgc tagaatcggg
    21481 aaaatcatac gtagtatatt tggcgaacag cctgattctg tagatattta tctatatgaa
    21541 tcatttgcaa aaacctatcg ggggcacgga acagatattg cattagttgg cggcctgtta
    21601 gggatggaac ccgatgatga acgtttagct gattctttga aattggcata tgaagccggt
    21661 atggaagtct tgtttgttcc taagagtgaa aaagccgaac atccaaactc tgtaaagatg
    21721 cttctaaaaa aaggaacacg aaagctttca gtcactggca tttcgatcgg cggtggcaat
    21781 atccagattt ctgaattgaa tggattcaaa atttctttaa gcatgggtac tcctaccttg
    21841 atcgttgtcc accaagatgt tccaggtatg attgctaaag tgactagtat actttctgag
    21901 accaatacga atatcggcac tatgacagtg acacgggaat caaaaggtga aaatgccatt
    21961 atgataattg aagtagatga tcctcaagta gaagaaacgg taaaaagact caaacagctg
    22021 ccaaatatcg acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt tttatcgatt
    22081 gaagaattag tagatcaagc aaaagcttat ccaagtgtag cagaattgat gattgcagtt
    22141 gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg agatcatgga aaaaaacctc
    22201 tcagtaatgg aacaatcaat caacgaggga aaagaaggtg ttacctccgt cacaggaatt
    22261 accggtggtg atgcaaaaaa attagatgat tatttaaatg aggcagactt tttaagtgga
    22321 gaaacaatct taaaagctgt acgcaatgct gtagctgtta atgaagtcaa tgcaaaaatg
    22381 gggttgattt gtgctacacc gactgccgga agcgcgggtg ttgttcctgg tgtgctgatg
    22441 gctgtccgag atcggcttga tttgacacat gaacagcaat tggaattctt atttaccgct
    22501 ggcgctttcg ggttagtcat cgcaaacaac gcttctatca gtggtgcaga agggggatgt
    22561 caagcagaag ttggttctgc aagtgcaatg gcagcagcag ctttggtttg tgcaaaaaat
    22621 gggactcctc atcaagcagc acaagcagta gccattacat tgaaaaatat gatgggactg
    22681 atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa tgctttaggc
    22741 tcttctcaag cttttatctc tgctgatatg gcattagcgg gtatcgaaag tgtcattccg
    22801 ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc gattttcaaa
    22861 gaaacagcag aaggtggctt agctgttacg ccaactgcta aacgacttgc ggaagagatt
    22921 ttaaataaga gggcgtgata aagctcctta atttcgagta ttaagagatt agttatcttc
    22981 ataacaaaaa acgaagagag gactaattgc ccttctcttc gttttttatt aatccatcaa
    23041 tttcgtatat tttacttcat cgatcgtttt tgttcctgca agttgcatcg tgatcgacaa
    23101 ttctttgttc aggtgttcaa aaacagatgt tactccttct gccccaccta gattcaaccc
    23161 ataaatcact ggtcggccaa tcgcaactaa gtcagcacca cttgccagag ctttgaagac
    23221 atgctctcct cgacggacac cagagtcgaa aatcactggt acacgtttgt tgactacttc
    23281 ggcaattttt ggcaatactt caaaggacgc aggacctcca tctagttgac gtccaccatg
    23341 attggatacc cagataccat ctgcccctgc agagatagcg attactgcat cttctggcga
    23401 ttgtattcct tttacgataa ctggaagatg ggtgatttct ttgattgttt tgatgtcatc
    23461 tggtgtcaag ccttgttttg cagctgcata aatttctgcg attccttttc cttcgccatt
    23521 accacttgca ctttgttcgc tatacgcagc taagttaggc attggtaatg gaaattggaa
    23581 ttgattgatg acgtcttctt cacggtaacc accgagcgtt gaatctgccg ttaaaatgat
    23641 agctttagcc cctgcttcta ctgctttgtt caaaatgaac tcattgaatc tgtcgtcttt
    23701 actcatatat aattggaaaa actgcggtgc gcctggcgct gcatcggctg cgtctttgat
    23761 tgtcgtattt gcataagtgc tgatggaaaa gattgatcca gcagcagcta ctcctttagc
    23821 tgtatcagct tccccttttt catgtgccaa tccttgggcc gctgatggcg cttggataat
    23881 cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt cgattccgcg
    23941 taatatccgt ggcatgattt tctttgtatt aaaagaagtc gtattttctt tcatcgtcca
    24001 ctcatcttca gaaccaccgc gaatatagcc aaaagctcct gcttccatgc gctcttttac
    24061 acgaccttct agttaggcaa tgtttacaat atcgattgga tgttcttcat tacttgtatg
    24121 ataagtcata gtagatcctt ctttcatctt cttgattaca tataaatcat actaaactta
    24181 caaaaagtaa gcaaacattt actctcagat acttttatcg ctattctttc caaataaatc
    24241 gacttacaag ctgatctact tcttggtttt catgaagcat actatgagag gcattttcac
    24301 ctgtcactac tttttcctct atttcatttc cgcgttgctt caacaacgca tagaccgcta
    24361 atgcactgtt taacggtact gttccatcac tcaagtctgt ttcatctagt tgaccagcaa
    24421 tcaagaaaaa tcgagtggtt ataggaatat taccgatcat ttgctggtaa tcttgatagc
    24481 ggttagaaac gacagcaggc ccctttgcta actcatctgt cagagactgc gcactatcat
    24541 cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt ggcaatgacg
    24601 tatcctgtcc atacgttcct aaatagcgta gtgcactaac tcctcccatg gaatgcccca
    24661 ctatgttgac tttatctatg ccgtagtttt ctttcagata caaaagacaa gttttgatcc
    24721 attctgcttg attccactcg ttatttttat tatcttcaaa caatacttgg accatcggat
    24781 tgtctgcttt tccagacaaa tctcctgttg cttgtatttg tccatctgtt cctactgtta
    24841 aaaccaattc ttttactgct tgctgatttt tttccaaacg ccgcaacatc cctttaaagg
    24901 aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt ttttctatca
    24961 ctttactgga ttctgccgtt ttttctttat cagaaacttc agtagtattt atcttcgtcg
    25021 tctggcaacc acttaacgca aacaaactga taaggactaa agctgctgca gttgcacgtt
    25081 tgcataaatt tttgattggt tctgtcatac acctcttcct ttccatatag aaaaaagaaa
    25141 gacttgaaag gccaatcata tgatcgacat ttcaagtctc tttattaatc gattttatct
    25201 acttgatcga aatcaagttc tgtactggtt tcacgtccaa acatatcgat gttgactttc
    25261 aatttttctt tttctccgtc aacttctgtt acttgtcctt ctaatccagc aaatgcgcct
    25321 tcgatgattt tcacgacttc gccaacttct acttctagat cattttgtcg caggctcata
    25381 ccgatagaac gcaagatatg attgacttct tctggaagta atggtgctgg tttgcttccg
    25441 gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac ataccatgaa
    25501 gcgtctgtca tgatcatttc aacaagtacg tatcctggga atgttttatg aacgatttct
    25561 ttttctttcc catttttgac ttcagtttct ttttcttcag gaacaacgac acggaaaata
    25621 aagtcggcca ttttcatact ttgcgcacga gattcgatat ttgcttttac tttgttttca
    25681 tatcctgaat atgtatgcaa gacataccag tttttttcaa aagattccat tgaaggctcc
    25741 tcatttcatt gtaaaataaa aaaacctcag tacacccgaa gtttttcttt cgcttcatta
    25801 tagcactaaa agtacgttgt ggctagcgag ttattttaaa atccagccaa ataagctttg
    25861 gatggctgta tccattacgt aaaacatcaa tccgaaaata atagaagttt cgatgactac
    25921 taacgtatct ttacggagtt gttttctgct tggccaagtt acttgtttga tttcatcttt
    25981 tacgcttttt agaaatttca tgagacaacc tcccttgcta ctgtttattt cgtttcttta
    26041 tgtaatgtat attgattaca atatttacaa aacttattga tttccaaacg ttcgccgctt
    26101 tttccttcac tgactgattt tgtgtagttg cgagacccac aaacggaaca agcgagtgct
    26161 gcttttttct ttgccattta cttctgcccc cttgctgaga atcagactta tacttcataa
    26221 cagtatcacg taacttttta cttgtcaatt tgtgaaataa actatctatt gttttggata
    26281 aaattatgat atgattaatt tgaaattaag ctaaaatgag acaattggag gtatagcgat
    26341 gctattaaaa acacttgtat ataagaaaaa agatctgaca accgttacag agactagtac
    26401 attagaagaa gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc cgatcttaga
    26461 ttcttcaggt aatattttcc gtggaaacat ttacaaaatg catatctatc gtcataaagc
    26521 aaatggcggt gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta caaagttcat
    26581 ttacctggat acttctttct tcaaagtatt ctttacgatc aaagagcttc cttatattgc
    26641 tgtccttgac agtgaaaatc atttttatgg tattttgaca catagtacgt tgcttaatat
    26701 gctctctcaa tcttggagcg tagaacaagg aagctatgta ctgacaattg cttccacagg
    26761 aaagcaagga gatttggctg ctattgcaaa aatcatttca aaacacagca gcattgccag
    26821 ctgtattact cttgatatcg aaaaagacga atacattcga cgtacattga tcacgctacc
    26881 ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga aaaagaatta
    26941 taaagtggta gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa gcgaaaaaag
    27001 aaaacagcta atctgctttc ttttttcgct ttttataatt agacacgctt ttcaaaatca
    27061 agcgcgcgat ctaaataata catcaatggt gctgaaacag ccatgacgat aaactcactt
    27121 aatgcaagcg tgccataggt tgtccaaaat gcttggccac ctgttggaac taacatataa
    27181 gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca atactaaacg catctttaca
    27241 tttttcactt tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt ttggccgcca
    27301 ccaaaaacca catccatgat accaaaacca aagaaggcat tatagacgat gaccccgcct
    27361 aaaactcccc aaagcaattt acggttaaag acaactaaat gattcaaact ttctgaaata
    27421 cgaaactgga tcggccctga ggatacaggc gcaactaaaa tagttaacgc tagataaaga
    27481 gccatgatga tgccatttac aacgattacg gataaccgtt gtctgctttt cgttttttgc
    27541 attttctctc ctcttttctc ctagtttttt taacgcggga tggttgatga accgcggtta
    27601 tgtgaaacaa acaataatta taacagactt ttcatttatt tcaatccgtc ttatctgctt
    27661 ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg atatggtata
    27721 aagtaagtgt aaggggtcat aagaattccc agtaaagcag acgaagtagg atctgcaaga
    27781 acagaattgt tgtgaaactt aaataaataa aaaaggatgt gggaatttga gcaacctctt
    27841 actagagaac ggtctagcaa gcagctgaag atctaagtgg acatgccgaa ccttggaact
    27901 tctccttacg taagtaatgg atcatgcgct tgctaggctt ttttgtgttt ttcggtcttc
    27961 ggaccttttt cattttccta gacttctgct ataataacca agaattaatt ttgtaacaga
    28021 tttttttgaa aaaacaagtt tctttcattc aaaaaataga aaaagcttgc taattattgg
    28081 ttaaccagca aacaggagta ttaaaatatc ttgttttatt gaatagcagg aggaaatcat
    28141 gaaattccat tatcatatat tgatcaatcc atcggcaggc agcggaaatg gctataaagt
    28201 agcagaaaga attttaccgg tattaaaaaa taagcatatt gactatacac ttcattatag
    28261 tgaatacaaa ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa cattgattcc
    28321 ttggatagaa gaagaacaga aaacaatcga tactttcccc cttttgatca tcgtaggagg
    28381 tgacggcacc ttacatcagg tacttgatac tttctatcag atggaagtag aattcccagt
    28441 tgcttatatt ccagcaggtt ctggagatga ttttgctcga ggggcaggcc ttccaaaaaa
    28501 tcccaaaaaa ggacttcagc tgattttagc agctcaatca ccagaaaaag tccacataat
    28561 ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt tcggtatagg
    28621 attagatgca gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc gattgaacaa
    28681 atacaactta ggatcatttt cgtatttatt ctctattctc cgtgctttat tcactcaaaa
    28741 aggatttcca attctggtag atgcaggagg aaaacaatta agtttttcaa acgcattttt
    28801 atgtacagca acgaagcatc ctttttttgg cggaggaatc gcgattgttc caactgctga
    28861 tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga atcaatttct ttaaaatctt
    28921 gtggctcatt cttttactta tacagaaaaa acaattgaaa tcgaaatatt tccatcacta
    28981 cacaacaaac agattacgta tcgtttcaac aacaccgcaa catggtcaag aagatggaga
    29041 agaaatggaa aaacgacctt ttgatatcac aataaccaca actaaacagt tgttttggtg
    29101 ttaataaaag aggttttgtc agaagctaca tctggaacac cgtttattcg gaaaaaagga
    29161 ctgtgacacg acttttgtca cagtcctttt tttactatgc agttaattct ttgcccaatt
    29221 gggttgccat cgatagtttc tttctttacc caaaataaat tggatcattg aaaacaccgc
    29281 agcaaaagca actaagacac aaccattgaa ccaaaaagat tctgcaaatg tttgattgta
    29341 actgagtgtt tcactgatca agttattatt ttgaacaaac caatatgttg gtgtgaatgc
    29401 cgctattttg ctaaccattt ctggcaagta ttctgctggt acaaacaccc caccgataaa
    29461 gcaactgcct gtgataaaaa tatctttgat cccaccgctg gtttctgagt tttttaccaa
    29521 tgaagtgata caagccgaaa agctaatgat cggcaataaa aacaagaaag tatttaaaat
    29581 atgataccat accgttatat cccacgaggt gtggatcact aacagaacca ttagaagtaa
    29641 gaagccactg aacaacagca gcgagtaact aatcaaacta aaggttactt tgcgattcaa
    29701 gtttcgtttg gtaatgggcg cactctggtt tcgtttactg atctctttgc gattaaatgc
    29761 tagattgatg acgctgaaac cgctaaaaat ggatagaaac aacccataag acaagaaatt
    29821 aaaggttgtg cccgtcaaca gctttttcat ccgctgacta tatcccgcac ttagctcaac
    29881 ttctccagta accgagagtg ccatttgtgt ctgttccata gctgcatcga ttgttttatc
    29941 ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg taacaactgt
    30001 tttagaaaag gcatctgggc gactctgtat cttgatcgtc ggcatctttc ctacttcgac
    30061 ttgttcggta aagtctgcag gcagatgtaa gataatgttt acacgattaa aataaagagc
    30121 atcatcgatt tctttttgtg aggttccatc caatgtaacg actgtttcct tgtcattcaa
    30181 atatttacga aggccatcga ctacaggcga ctcttcttca ctaatcaagg cgattttcgc
    30241 atcttgcaga gaggtatttt caacatcaat attttgcgca aaaaagaatg tggcaacaac
    30301 agtgataatt acacctatca gtaaattcga tttatttgct ttaagcaatg tccaaatcgt
    30361 tttataaagc gtcataacgc acccctcttt ccatccaagc atttcctaac gcaaaaagga
    30421 aaataaatcc gatcaaccat agaagattgg tataaaaact agccaaatca gtgtagaaat
    30481 aaagtttata caaactttcg ctgatcaagt tgacgagatt taattgacca acaatcggaa
    30541 tatgaagatc aatccaatac ttcacttgtt ccgaccccat cataccagca aaaaagctca
    30601 tcgcaatcgt cactgaaatc aagatgctat ccttttgcac cagattcatt tttggtatca
    30661 aattaccaac caatgtacct aacaaaatcg cacacaacga ccccagagcg gtaacaatca
    30721 tcaaccattg ccaacgttca ccaaagtcta cttgatagat aaaacgaaag acagctaaca
    30781 taatataaat ctctgtcaaa acaatcgtaa agcttgctgc caaattagcg attgaaacta
    30841 acgattgctt tctaggaatc aaggacaagc gaataccatt tgacgattga tttgcttgct
    30901 gatcctgtac attgcggacc ccccacatcg tcccgtagag aatcgccatt ccaatcagag
    30961 tgaagaaata aaaacttttg aaactcatat tacgacctgc atgaacttct tgaaaagata
    31021 cttcttgttc aaaaacagtc atgatgggcg cgagatcaac gttctccact tctgccactt
    31081 tactcgaaat tataccaata cgctgtaaaa attgatccat gacgtttttc atcaacgtct
    31141 gactcattcc tgcttgaccc acatggagcg aaacagatga accatcaatg agataaaaac
    31201 cagccaaatc atctgcattt agcttttcat tcgcttctac ttccgatagt tcctgtactt
    31261 caaataatgg tttatcattg acttcgactt gttctaagac tgtggtaaaa ttttccgaaa
    31321 gcgctgtttg atccgctttg attacaattc cagtattaat cgtttcgaga ttgccacctt
    31381 gatcaatctc gccaaaggcc gccataaaca tcaatcctaa aagaattgga aaggccaatg
    31441 tccaaaacaa catggattta tttcgcagca gtatttttgt gcgatattta taaaaatgcc
    31501 aaaacattat ttcccctcct ctagtctctc aaatccttgc cagtaatctc taaaaagaca
    31561 tcattcaagg acgcttcttt ggttgctatt tttccgtaac tgatcttttc ttcttgtaag
    31621 attgtaagaa tcggcaacaa tactgtattt agttcagtag ttgttacttg gagctgacct
    31681 tttttataag cgattccatc agaagctatt gttcgctgac aacacgtttt ctgggagtaa
    31741 tgggatgtcg atcgtcaaac gatccgccgt ccgtaccata tcttttagag attctttggt
    31801 tcctttagca atcacctgtc cttgatccat gatcacgatc tggtcgcaaa gaatttctac
    31861 ttcttccatg taatgtgttg tatagacaat ggttgctcct tgtcgattca gctccttgat
    31921 actttctaaa attttattcc gactttgggg gtctacagct actgtcggct catctaaaaa
    31981 aattaaccgc ggcttgtggg caatcccaca tgcgatgttc aaccgacgtt tcaagccacc
    32041 actaagttct cctggacgaa acgaaacata tttttctaat cccactaatt gaatcacctc
    32101 ttcaatcaag gctttccttt tctctttttc ggaaacatat aaaccgcaaa aataatcgat
    32161 attttcttgc acacttagtt cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat
    32221 tgccttgaca gcataatttt caggagtcat tgttgtcccg aacaatttaa tggttccttt
    32281 gtcataattt aacaaagaaa gcatacagtt gatggcagtc gatttcccac tgccgtttgg
    32341 tcctaacaga cctaagactt ctccttcttg tacttgcaga tcaaaatgat tcaatgctac
    32401 aaaatctcca tagcgtttga ctaatttatc aatttcgata atcatcatga aaaactcctt
    32461 ccaaatttct tgttgatctg actataataa agtgcaagaa aagaaactag tgacgattgg
    32521 ctaaaccaca catgacattt gtcacaatga aggagtctgc tatgcataaa tctcggctac
    32581 tcaacgaaac tagctttcta ctggttatct cattgctttt gctggtaagt caaggattcg
    32641 ttgctatgca agttatctat ttattgatcg ccgttttatt cactggtgtc agttatttac
    32701 tgcctacaaa gccaaattgg attttccaag ttattctatt tcttttatgt gcctttttac
    32761 ctgatcttgt cctgtttctg ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc
    32821 ttgattactt gtttttgtta ggttttttgt tgatttcgac ggttggtaat catcccccca
    32881 tcaaggtcgc ctttgtcgta attttgctgt ttttttcctt ttacttacga aaaaaagatc
    32941 aacgaacgac cgatcttgag aaaaagtaca ttgtctccaa agatactcat tgggaaaaac
    33001 aacaagagtt atcggaacaa aataaagcat tggccctgtc acaagaaaca caattagcct
    33061 tgcaaattgt ggaagaacgg aaccgaattg cccgagacat ccatgacaat gtcggtcatc
    33121 tgctgtcaag tgcgatttta caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac
    33181 taaaagaacc attagaaaat ttaagtgcca ccgttcacac tgggatggat cggattcgtc
    33241 aaagtgtcca tgacctccat gagacatcga tctcttttca acactcctta gagtttctga
    33301 tcgctgattt tccttcttct gttacgattg aaggaaactt gtttgaagga ttgaatgaaa
    33361 cgcagcaaaa ctgcttctta atggtcatca aagaagcctt aaccaacagt atcaaacata
    33421 gtcatgccaa aaccgttgtt ttacattttc gaacgttgcc ggccttttat cgcttgcaaa
    33481 tcacgaatga tggcaccagc ccacaaaaaa attctgctcc cggtattggt ttgaaaacga
    33541 tgcgtcaacg tgttcaagaa caaaagggac aagtccatat tttccaagaa aaccagcaat
    33601 ttcagttaac gatcatttta ccaagggagg gacaccaatg ataacagtag tcattattga
    33661 gatgatccat ttgtgacgaa atcgttacaa accattttag aaagcacgaa agagatccgt
    33721 gtgttaggca ttggtcattg tgccaaggat gcgctagtct tatacgaaac ccatcgtccc
    33781 gatgttttgc tgacggacat ccgcatgcct aaacaaacgg gcatcgatgc tgccaaagaa
    33841 attttggcta aatttcccaa agccatcctt ttgcttttga cgacattcaa agatgaagaa
    33901 tacatccgcg aagcattttc catcggtgtc aaagggtatt taatcaaaca gaacttgcaa
    33961 gcgattattc cttctgtcaa atccgcttat aatggacagg ccgtatttgg taatgaaatc
    34021 atcgagactt tcactcaaat gatgaagaat aaaccgacga ttgatagtca tgcttatgct
    34081 tcttttagtg aacgtgagct tgcaatcatc aaagaagtag ctgccgggaa aaacaataaa
    34141 gaaattgcag atgccttgta cttgagtgac ggcactgttc gcaattacat cagccaatta
    34201 ctagaaaagc tagatttacg ggatcgcacg caattggcta tctattttta tcagcatttg
    34261 caataagtct tatctaacac aaaaaaacct gaatgatgct ccttcgcatc attcaggttt
    34321 ttttcacatc ttctattcaa ttgtcccgca aaccatttcc tcacgattga aagaatcaag
    34381 gattcgcagg gctgccagga ttcgttggat tgttcggcgt gttaggcgga aaaataggcg
    34441 ccttgtctaa cgttttttct agttttgatt cttcacgatc gtctcgttct acatcaggac
    34501 tttcattcaa tttgaattct ttttccatct catctaactt tttaaagtca ttgccagatt
    34561 cttttgctaa tttcgctaat ttatcttcgg tttctttagc cataaagtaa cgcccccctt
    34621 ttgatttcag cttagcatat cacactgaaa aaacaaaaga aaacgctaac caacgatcat
    34681 ttttagctat ttctcatctt taaaatcaag cattttttgc cggtataaaa tcgtagggag
    34741 aactgttttc cataccaatc atcggatcta ctaactggtt atcgatcact tctgctgtaa
    34801 gaacgaaatc tttagaaaca gcaggttcta cctcttcttt cttttcttct ccaacaaatg
    34861 aagaaaacag atctggttcc actgtttcca ttacttggtt tttcgtttca ttcgtcccac
    34921 tgtcatcttc tacaggtagc ttttctttta caacccgtgg ctcttttgta tagttcccat
    34981 cttcaaatgg atcatttccg accaaagaat cttcataagc tgctagtttg gtccggagag
    35041 tgatattcat tttatcatta tccatgatcc ttgatttcaa cgtagtcatc cggcttgctg
    35101 attcattcgc tacacaagtg acataggctt gctcttcccc taacaaaatc aacagttcct
    35161 tactgcgagt cactgccgtg tagagcaaat tacgctgaag catccgctga tattgatgga
    35221 ccatgggcaa aataaccatc ttgaattcac tgccttgtga tttatggatt gagcaacaat
    35281 aagaaagagt gattttattc cattcgtttc ttttataagt gacctcattt gcatcaaact
    35341 ggataactaa ttcatccact ttatcttcag aatcttttgc caagatgata cccgtgattt
    35401 ctcccatatc tccattgaat acattcaatt cgggagtatt gactaattgg aggactttgt
    35461 ctcctatccg ataaacggtg tcgttccaat gtacttcctt ttttccaggt ttccctggat
    35521 tgaatatctc ctgcatcatt ttatttaacg catcgatccc tgcaggtccc cgatacattg
    35581 gtgctaatac ttggatatct tgtgcacgga atcccttatt tttcgctttt tccacaattt
    35641 tagagatcaa gggttcaatt tgataagcat cacttcggaa ataggaacga tctttttgat
    35701 tctttgtgaa atcttttggc agacgtcctt ctttgatctc atgggcaaga ggaataatac
    35761 tagaaccatc accttgccga taaatctgat tgagttccat tttgggaatt tgttcgattt
    35821 ccaaaagatc atggagtact tgaccaggac cgacagaggg aagctgatct ttgtctccta
    35881 ctaagataac ttgcatattc gtcggaatcg ctttgaataa tgtgttagcc agccatgtat
    35941 cgaccataga catctcatca acaatcaaaa gtccgccttc taattctttc gtactgactg
    36001 aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg gatcgtactg gcaggcagac
    36061 ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg tgctgcaagt agaataggaa
    36121 aaatttcttg ggtatatttt gaaggatcca attccagtcc gttcaactct gcaaaaagat
    36181 tgacgattcc gttgatcaca gtagttttcc ccgtacctgg tcctcctgtt aaaataaata
    36241 gaggggaacg gattgcttca gcgatagctt gttcttgcga aggtccatac tgtatgccga
    36301 atattttttc gatacgccga atattcttct gcacttcttt ttcaggataa tggatttctt
    36361 ttttacgact gagaaggcgc tggatcgaat taccgatccc ccattcagaa aagtacaaac
    36421 tgttttcgta aagattagtc tcttcttgtt gtatctttcc ttcttcaaca agttggataa
    36481 ttacagtagc aacctgatca ggctggattt caattgggcg acttgtttct aaaatacgga
    36541 tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc tcctgtttga actgcatgct
    36601 gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg tgaatctgct gcaataccga
    36661 tttgttcggc tagattatct gcacgtttga acccgattcc ttctatgtct tcgactagct
    36721 ggtacggatt ttcttcgatg atatccaaag actcgttctt ataggcctgg tagatactga
    36781 aagctaactg actaccgaac ccgtaacgat tcagtcccat gatcacttga tccatcccgt
    36841 ggtttagccg aatcgtctca gcaagcattt ctctttttgg cttcgtcaaa ccggagattt
    36901 gttttaatac cgaaggatca gcaatgattt tttcgatagc atcttctccc agcaattcta
    36961 caattttttc tgctgttttc ttcccgattc ctgggaattt ttcgctggaa agatacgcta
    37021 cgatcccatt tgcagttgtt ggcttttctt gctcatatct ttcgagttta agctgccttc
    37081 cgtatttagg atgatccact aattctccat aaaaacgata agtctcattt tcttgaatat
    37141 cgccgaaact tccggtgact acaatctctt tttcacggta atcagctgtg gtttcactga
    37201 tgtgtaccaa aagcactttg taaaaattac taggattttg aaaaaaaatc gcggcaactg
    37261 taccaacgaa ataaggcatt tcttctggct gcatcggaag ccgcctcctt cttctatagt
    37321 aaaggttctg gtgctgtttc agttcctaaa aaatctgttt ggttccatac tttcagttca
    37381 tatggcaata atctgcaagg ctcttcagtc tctaaaatcg tcaaactact attgaacaat
    37441 ccacccattt cacgcaactc acttagctct tttcctgtca tccattgaat ggctgcagtt
    37501 aaagaagctc catgtcctac gaataaaagt gggccatctt tatgttgggc aaccgcatct
    37561 gctacaacag tttcgatacg tgtgattgcc gattcaatcg tttctccgtg aaaaggagaa
    37621 ggatcataca gatctaaatg attacgcaaa ttcggcagat tatccggata tttctggtac
    37681 atttcatcga tcgactgacc ttctaaagta cccagcccta gttctcttag cttatctgta
    37741 taaaaaatct ctaccggacg agcaagctct ttgttgatac cttctgctgt atcacgtgct
    37801 cgctttgctg tactggaata aattgcttta aaaggaacat attttacctt tttcccaaat
    37861 gctttgattt cttcgtaact ttgtggcaaa agcggagaat cgccattgct cccttgaaaa
    37921 cgtctttcct gattccattg tgtcttccca tgtcgggtaa aatatagttc catacgcagc
    37981 aaactcctgt ctttctttta ttttactcta taataaactg tctaatccag cttgtcattt
    38041 tcatagtgat gcgtcaattc taaattaggg aatccttgct ggcgaagtgc ttcatacact
    38101 atcattgcag ctgtattcga taaattcaat gagcggacat gctcatcatt catagggatg
    38161 cgtaaacact tttcttcgtt ttcacgcata aatgtttctg gcaaaccggt tgtttctttt
    38221 ccaaataaaa agaagtgctc ttctccatca gcaaaatttt cgtcactata tactttatgg
    38281 gcaaattttg agatcaaatg tagtttacga ttacctaaaa atgttaaaaa agcttctaaa
    38341 ttactgtggt aagtaatatt tacatcattc caataatcca accctgcccg ttttagatgt
    38401 ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa gcggagtatt cgtggctgca
    38461 cacgtacggg caatatttcc agtgtttgca gggattaatg gttcaaataa aacgatatga
    38521 ttcatcagga tcattcctct catatttatg ttcgtttcta ttttctgaca atttgatttt
    38581 tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc gagtcgttac gttagtgaag
    38641 ctcttctcac taacttttat cagaacagga acctgataaa aaccaatgaa aaaacaactt
    38701 gttgctaata taacaccctc aacttttttt tgcaacctct actcattaaa aactgctaga
    38761 aaaacgtttt ctcttagttc cttttaataa ttctatgtca attgtaatct tcttcaaagg
    38821 attcttatat aagtctgctt tgattcgctc ttctacctgc cattctaaag gtgacatggc
    38881 caaaagatct ttttgatttt cctctggtat ctcaaattca taggttacac gctgacgttc
    38941 agttaatttc attgcttcag caaacttggc tactacacgt tcattggaat aactctgttt
    39001 ttcaggttga ttcggataaa aagcctgacg tagttccttt aaataatttt cttgtggcac
    39061 caccttcaac acttctccat cttctgagag tacacgttgg aattcttggt aatgagaagg
    39121 cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca aacggcaaat tggtcaaatc
    39181 agctacacac caaaatgctt cggcagattg ttctgtagct gcgtagactc cttcttttga
    39241 aatatcaaat ccaacagcat tttgaatctt tttcaatttt gaaagttcat tcaagaagct
    39301 accttctccg cagccaacat ctaatagcga gacttcttca ggtaaatgct gcgcaatagt
    39361 ctcaataacg ggctgataca tgccagattg gatcattcta cgccttggaa cgaacattcc
    39421 ttgatcgtat tctgttttaa ccgcatggga taaaaaataa agtgttcctt ttttggaaag
    39481 gtcaaaacgg tgattatttt tgcatatcaa gccattatca gactttttta cttcttcttg
    39541 gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa aactggcgtc ctcggtcaat
    39601 tttttttaac atagttcccc ctcatttctg ttttatctta ccgttagatg ctcactttgg
    39661 caactgctaa aaatatgata caatgcctag aagaaagtga aagataaagg agaagattag
    39721 atgatcaaag aattttgcgc tgaaaactac acatctattc ctctagcaat tgctagagga
    39781 gccgatcgga tcgaattgtg tgacaactta gcggtcggtg gcacaacccc gagtactggt
    39841 gtgatcgaag aaacgttaag ttatgctggg gaaaaagata tccccgtaat gacgattatt
    39901 cgtcctagag ggggcgactt cgtctataac gatatcgaat tgaaaatcat gcatactgat
    39961 ttgattgaag caaagaaatt agggacggat ggcgtagtat ttggttgtct tactccttca
    40021 ggctggctag acgaagaggc cttagaacta ttgatcgaaa cagcagaagg gttgcagatc
    40081 accttccata tggcttttga cagtattcct gaagatcggc aattcgaagc aatcgattgg
    40141 ctggtagaac atggtgttga ccgtatcttg actcatggcg gtcctgctga caaaactatt
    40201 gtagaaaatt tcccacggct taaagaattg attgcctata cagatggccg cttaatcatt
    40261 ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg tagctaaaga attgaacgta
    40321 agcgaagtac acggaaccaa aatcgtggat cttcagttat aatcttataa acaaaaaaac
    40381 atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt tttttacata aaatagccca
    40441 taaataaaga agcaatattg aaataaatca gtacactcgt caaatcgctc aaagtcgtga
    40501 taaagggacc gcttgctact gctggatcaa atcctagacg atccattagc atcgggatta
    40561 aactacctgc aagattcgcc actgtgattg caaatagcat agccatccct atgacaaacc
    40621 ccaagataaa attatgtttc caaaaaccta cgattagaaa aatcgtgatc cccgtaacag
    40681 ctcctgtgac aacgccggtc aagacttcag ccaaaacaag tcgaacaaat ccatgatctt
    40741 tctcatcaga aatcgcaagt cgacgaacag ccacagctag cgactgggtt ccagcattac
    40801 cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat actcgcttca cttaccaagt
    40861 cttcatagtg actgattaaa gaagcggtcg acatacctaa aaacaacaga gtgatcagcc
    40921 aaggcaaacg tcgagacgct gcctttactg gattttcatt aatttcttcc acattgaccc
    40981 cggcaagtcc tgaataatcg ctagcagctt catcatcaat aacgtcgatg atatcatcga
    41041 ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac aggaagagct aagaagtcat
    41101 aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc tacatgaact gaaagcactc
    41161 gttcgctcat cagatcagaa atcattgcgt cgtcatcatt cacgattagg tcgcgcagag
    41221 aaataacccc tactagctga ccttcttgat taacgacata gatgtaatag attgtttcag
    41281 ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg aacagtttga ttggctacga
    41341 tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc atcctcgtaa tggagaagtt
    41401 ccttgatttc actggcatcg tctgccgaca tcaaacttaa atatttagca atttgctttt
    41461 tatccagcgt attaagcaag tcaacagcat tatctgtata catctcagaa agcatatcag
    41521 ctgcatagct aggacgcatt tcatctaggt attctttcat atgttcgttg tcttcttcaa
    41581 tcacatcgaa catatccgct aattctttag gcgacagata ggaatagatc agctgcctgt
    41641 tctcttctgt aagagattgg tagaattgcc cctgctcata gatatgcatc tctaaaaaac
    41701 tgtttctgaa tgcctgaata ttattttccc ttaaatcttg agtcaactgg gcaaatcttt
    41761 cctctaattc ttgattttca tccaaaccaa tctcacctcg ccttattctt caaaccgtct
    41821 cacaactgcc tccatgtctg cggctaaagg ctgcttgaac tccagcaact cttctgtaaa
    41881 tggatggtaa aaacgcagat cataacaatg aagggcttgt ctttttattc ccagatccat
    41941 tcgcccgcca tacatatcat caccgagcag ggcacagcca attgcggaaa aatgaactct
    42001 tatttgatgt gtccttcctg tatgcagctg gatatcaact aacgcgattt ctgttagtcg
    42061 cttctctatc cagtactcag tctgtgccga acgtccttca tcagagacca ttcgcttcaa
    42121 tagcgaagaa taatctcggg cgatcggcaa atcgatatgt ccatgtgccg ataaatctga
    42181 aagccttcca ctgactaagg cctgatattt tttcacaaac ttcttttcac gaagttgcat
    42241 atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc atcaaaccag atgtatcccg
    42301 atctagtcgc gtcaccacat ggattacttg gttttcgtaa ccttgttttt tataataagc
    42361 ctttacacga ttagccattg tcccatttgg atggtattgg gcaggaatag acgaaacgcc
    42421 tgctggttta tttactacca gaacgtgttc atcttcatac acgatatcga tcggtgtttc
    42481 atccaaaagt acggtttcat gttctccttc agcaggaatc acaatggtta ccttatcatc
    42541 cttagctaga gaaaacaaga cattttctac ttgatcatta actttgattt gaccgccttg
    42601 aaattttatt ttcgctaaca gaccctttga gatccctttt tcttttaaaa aatattttac
    42661 ttgctgagaa tgatctttct cataatccca tgtaaactgc atatcaatcc tcactaataa
    42721 aggcatcttt cacacgatgc caaaaatgca tatgtcgata agaagcaaaa tggatccgtt
    42781 cttctgctat tcgataataa attgagcgga tatttgcttt tgcgacattc aattgatcaa
    42841 ccgttacaag ataatcttct gtattttcca attttatttc tagccattca tcatgggcaa
    42901 tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt cagtgaagca atctctgcca
    42961 actgaaaggc attgatacta ggatggatca cagcaccgcc gacactttta ttatatgctg
    43021 tcgaaccagt aggcgtagaa atcgtcaatc catctcctct aaaactttca aacagctcgt
    43081 ttttgatgta tacatcggct accatcgtac gattggcccg tttaatcgtt gattcattca
    43141 gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact gatccgaaca tccagcaagg
    43201 gataactaac actttgttcc cggttcgttc gtaagctgtc tactagttct tccagctcgt
    43261 aatcccgcca atccgtgtaa aaccctaaat gacctgtatg cacgcccaag aaacgcacat
    43321 cattcagaca atgactgtag tgatggaacg ccgacaataa tgtgccgtct ccgccgactg
    43381 agatgaccaa ttctggctca tgatcatctc gttgaatccc tgcttgttct aaaagtgcgt
    43441 tcaaacgttc tgtcacttct atagtctttg cttctttatt atgaacgatc gctactttca
    43501 tcccctgcct cctctctttc gctttctttc ttcaaaagcg ttcgatagta gacatcatct
    43561 gattttcctc ttccatggga gaataaccgc tgtgcttctt ggatttcttc acggatactg
    43621 gacatctctt catccaactg ataggcagct tctgccgcac gctgaagtcg ttcactcatt
    43681 tcttctggaa aaacgccttg atatttataa ttcagcgaat gctcaatcgt tgcccagaaa
    43741 ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt tcttctcacc gctgatcagc
    43801 tgtacaggat actcgatcac cagatgataa gaacggtagc cacttgcttt tttatttgtg
    43861 atgtaatctc gttcttggag aactttcatg tcttttcttt tacggatgat ctcaacaact
    43921 tgatggatat cttctacaaa ctgacacatg atccttaacc cggcaatatc agacatttct
    43981 tcttctaaac gtcccattgg aatatgccgt aattgtgatt ttgttacaat actatcgact
    44041 ggcttgactc tcccagtgac aaattcaatc gggacatgtc gattttgttc acggaactgt
    44101 ttgcggatcc cccgcagttt cactttcaat tctgatacag tttgttcata tggtgctaaa
    44161 aatgcttccc aatctcgttc catagctgat acctctactt cattaacatt cattgtttat
    44221 tctaccatat atgcaaagtt ttttcgtact cttgatttat gcattgacca aaaaaagaaa
    44281 attaggcaaa atagacaaaa aggagtcgat caaatgacac aaatgcttga aatcgaatat
    44341 aaatctatgc tgacaaagca agagtacgag atattcatcg ctcactatca attaacagat
    44401 aaagatttcc gtgtacagac taatatttat ttcgataccg ttgatgaaca gctgaagaaa
    44461 caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag ctgaatatac attaaaaaca
    44521 cctgctgaaa aagggcgtct cgaaacaacg gataccttta taacagaaga agcagaagca
    44581 tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt ttcaaaaact ttctgatttg
    44641 aatatcgatc ctgcttcatt aatccaaacc ggaaagctta caacaaaacg ggctgaattc
    44701 cctattgaag aaggactttt agctattgat gaaagctggg gagacaatct acacgatttt
    44761 gaactggaac tagaggtaag gggatgcatc tgtgggaaaa attgctttta taaatttttt
    44821 aaaacgtttt tctttacctt atcgtcctgc taagaacaag atccaacgta tgcttgaagc
    44881 taaaaattag aaacctttca ttttcacaag gcacttgagt tcattttttt acttttttct
    44941 gttaagttat aggatgtgaa agaatctagt cactagttgt atagaatcta ttttggggga
    45001 gattcatatg atcgaaatct atttatttgt taatccttta ggggaatact gtttcgaaat
    45061 ggaacaacag ttattacaat ttatcgaaga agagtacggg acaccatcaa aagaaaagat
    45121 gcaatttcgc tttcttcctt tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa
    45181 aggaatttct cagaacaatc ttgaggaacg taatcactta ttctcaatga cttattctgc
    45241 agcattggac tgcaaagcgg cgcagtttca aggaaagaaa aaaggccgcc gttttttgat
    45301 caaattgcaa gaagcagtcg gctgtaatga cataccatat tctcaagaac ttgctgaatc
    45361 catatttgaa gaaatcggcg gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt
    45421 cgttaaagaa gtattctttt ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca
    45481 cccttctgca gtcgtttaca attatgcctg cgaccgtgat tttggtgttt tagtagaagt
    45541 aaaaggtaca agtagccttt ggagaatcac agagctgtgt aaaacaacag ataactcata
    45601 tcaagttttt aatggtgaac atttacatac aaatgattct cagttccatt caagaactag
    45661 ccatttacac ttgctttcta attaaaaaaa tacgtcgact cccttacaag agagccgacg
    45721 tatttttttc gtttctattc gagaaaatca aaaatagaaa gccttataaa cgatcgatca
    45781 attcttctaa ttcatttaga cgttgttcaa acatcgacat tgcatcttca atatatgcag
    45841 cctgtgtcat atcaactcct gcttttttca ttacttccac aggataatcg ctgttacctg
    45901 cttttaggta agccaagtag ttctctagtg cctctggttc ctggtttagt attttcttcg
    45961 ccagtgctga agcagcagaa aagccagtcg agtattggaa aacatagtaa ttataataaa
    46021 aatgcggtat ccgtgaccat tcgaatttaa tttctgggtc ttcttcgact gctggaccat
    46081 aatattttgc attcaattta ccataactat cacttaggta ttcactagtc aatggcacac
    46141 ctttttcatc ttccgtatgc atgaaatgct cgaattcagc gaattgtgtc tggcggaaaa
    46201 ctgttccttt aaacccatcc aagtaatggt tgagtacata agcccgtaca cgagggtctt
    46261 tttctgtttc caataaatac tccgttagga tattttcatt cgttgtcgaa gcaatttctg
    46321 ctaaaaagat ggagtagtcg ccgtacacat aaggctggtt cgaacgagtg aaataactat
    46381 gaacactatg tcccatttcg tggacaagcg taaatagctg atccagcgta tcatgccaat
    46441 tcaataaaat atatggattt gtgtcatagc ttcccgaaga ataagcaccg cttcgtttcc
    46501 ctttattttc gacaacatcg atccaacgtt cagagaatgc tttttctacg atggccatgt
    46561 attcttcacc cattggtttc agtgcttcta aagctttttc ttttgcttct tcgtacgtaa
    46621 aggtaattgg agcttcccct aagaccggtg tataaagatc atacatgtgc agtttttcca
    46681 cttctaataa acgtttccgt aattccatgt atcgatgtaa caaaggcaaa tgcttgttta
    46741 ccacgtctac caaagtatcg tatacacttt caggaatatg attattgctc aaagacgctt
    46801 ctctggcaga gctgtaatta cggactttcg ctttaaaatt atgtcctttt atatgtgtgc
    46861 ctaaagtaga agcaaatgta tttctaaatt gttcgtaaac actgtacaat cccttaaatg
    46921 ctgcttcgcg cacccttcga tccgtgcttt ctagcaactg accatacacg ccatgagata
    46981 attggactat ttcaccattt tctccttcaa tcgttggaaa aactagatct gcattattca
    47041 aaacagcaaa tgtatcactt gaagcatcaa agatttcacc tgctccagca agaagagatt
    47101 cctgctcagc agataagaca tgggctcgat tatctactat ttgctggata taatggcgat
    47161 agacttccaa ttttggttct tctttgaaat attgccaaat ctggtcatct gacaattgca
    47221 atatttctgg ttcaaaccag gaaacggctt cactaacttt cgaaaacaga ctgcttgctc
    47281 ttgcataaag cgcttggtaa tctgtatttc cagtgtcttg atcgttttta aggtgcgcat
    47341 atacataaat gacttcagtt tggcgataaa ccctcaatac gaattcaatc gcatttaaaa
    47401 attgagaagc gccttgatca agtgttcctt tgtgtttttc agattgtttt aactcttctg
    47461 ataattccaa atatttctca tcaaactctt ggtcgcttga aaagatcttg gtcaagtccc
    47521 aagttaaatt ttcaggcaat tcttctcgtt ttggcaactg ctttacttcc atgaaatcca
    47581 ctccttttct tcccttagct ctatctatca aaatagatca tagtctaaca tccacatgtc
    47641 acagcatcta acttttctac tcattcatgt gtctgcttcc atcaatttta gcataactaa
    47701 atcttgtttt acatagaaaa ccgattccta gcctattttt atacttacta ttttttcaaa
    47761 gaagacaagt gtatgcattc gttaaaaaac tgctgataga ccatctcttc atcaatcata
    47821 ggaaaaagcc attccctttt atgttctttt atttggcaaa gccatgagtt gaaatcagga
    47881 actttctggt tttccaaggc ttcttcgtac aacattcgat aaagaaaaac ctgttcttgt
    47941 aaataaaaga aaaaccgact tttttgatag atccacgatg atagatgcaa aagatgttta
    48001 tgacgcaagt agcattgttc ttgtatggat actgtattct tttgcttgtg aataagttgg
    48061 cgtgtcagcc atttcttccc atcttcactc actttttcta tttttttcat ttggttcatt
    48121 ttaatttcgt tactagtgaa tatttcttta aggtccctac tagaaaaagt ccagcagcta
    48181 ctgcaaaagg aaatatgccc tgatacagtc tctgtaatat gatgatacaa ataaagcttt
    48241 tgtttcttga gatcagcttt ccagcaatgc actcctcttt ttctattata gtagcagcaa
    48301 cttttttcta tcaatgagaa ttgcgattgt ccaagaaaag aattaccaag tatccaccat
    48361 acttggtaac tgttattcaa ataattttgg gttctttcaa taaatctctg gtgagacagt
    48421 ctagaacatt ggatttctat tgctagattc gccaacaaaa tatccggcct ttgccgcaac
    48481 ctcggcaaat aggcttccaa aaatactggt tctactgact gtcccagcca atccatgaag
    48541 cattccttta actgtaagtg ctcttttgat tctctttcac taaagaccga acagttatct
    48601 gatttttgat gggcaaaatg agcacatttt ttctttccct ttttcaaaat cactcttttt
    48661 cgacagccac gacaataata tttctttcct ttttctgcat gtttagcaaa acagcactta
    48721 ttatattcat tcattgcaag cagcattttt ctccctctga acttatttac gtctaagaat
    48781 ttatttacac gaaaaaagag ggcgtgaaaa agctattctg catcaagtaa taagaacagc
    48841 taaacaaaaa tagttcccaa tatttttcgt ttggttgggc ttgttaccgc agatgcaagc
    48901 ttttgaacac atttatacga aaaaaagact gcgacacaat ttttgtcaca gtctcttact
    48961 ataatgctga tttatacaaa ataaaatttc gttcgctcga tagcattacg cgccatcaga
    49021 cattgtccat attcattcaa tgtttctggt gtacgatcgc tcttttcagc aaactccaaa
    49081 attcgagcaa tctgattctc tacatccgct tggttgagat tttccatcca gaagtgaacg
    49141 actaggtaat agcgctcttc gaataaatac aaatcagtcc aagcagactc tagttcgaca
    49201 tctgttgcaa gttggatcac ttgttcaaaa tcttcaaaag caaaagttgc ttgtctttcc
    49261 tgttcttcta gggcgcttcc agataattct tcctccggat cgctgccaat aatctgctga
    49321 cgaagaaagt cactcacatc ttctgaatca acagtagaat cttctgattg agaaatttct
    49381 tcatccggaa gatttttact gataaacagt tctaatccgt ctccttttgg caaaacttgg
    49441 aaagtgacag cttcgcttcc tcggaactcc tcttcgatat caacttcttc caaaatacta
    49501 taaaagaaat tttcgatttc tttgtgattg ccaagtaaat ccaaaaatga aacgccccgt
    49561 tcctctaaat cttcatgacc aattagtaca cggatcgtat tttcattaat atgttccatt
    49621 tccattttgc tacacctcac tttgccacaa tgtttaatat agattcattg tagcgaaatc
    49681 agcttaaatg taaagaaaaa aaatctcgct gttaagcgaa tttaataaaa ccagcggatt
    49741 tttcaacctt tcaagctatc ttatttaact ataacatttt tggcagacga atactatata
    49801 gccgactttt cagctaattt tccctctcta tttttcgtgc aatcatcatc ttctactacc
    49861 tttttataca tctaatcaag aatacttttt gtaaaaacaa aaaaacttgg aaaaactatc
    49921 tttgtaatag tcttt
//
LOCUS       DAYYBH010000015        41001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000015 DAYYBH010000000
VERSION     DAYYBH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.015"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACHAUG_000988"
     CDS             complement(38..1189)
                     /locus_tag="ACHAUG_000988"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8480096.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACHAUG_000989"
     CDS             complement(1202..1639)
                     /locus_tag="ACHAUG_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8480097.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACHAUG_000990"
     CDS             complement(1654..3420)
                     /locus_tag="ACHAUG_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8480098.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACHAUG_000991"
     CDS             3684..4361
                     /locus_tag="ACHAUG_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8480099.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACHAUG_000992"
     CDS             4364..5491
                     /locus_tag="ACHAUG_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480100.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACHAUG_000993"
     CDS             complement(5691..6131)
                     /locus_tag="ACHAUG_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8480101.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACHAUG_000994"
     CDS             complement(6142..8175)
                     /locus_tag="ACHAUG_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8480102.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACHAUG_000995"
     CDS             complement(8177..9880)
                     /locus_tag="ACHAUG_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8480103.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHAUG_000996"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHAUG_000996"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8480104.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACHAUG_000997"
     CDS             complement(10963..11988)
                     /locus_tag="ACHAUG_000997"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8480105.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACHAUG_000998"
     CDS             complement(12197..13066)
                     /locus_tag="ACHAUG_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8480106.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACHAUG_000999"
     CDS             complement(13525..15417)
                     /locus_tag="ACHAUG_000999"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480107.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACHAUG_001000"
     CDS             complement(15414..17141)
                     /locus_tag="ACHAUG_001000"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480108.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACHAUG_001001"
     CDS             complement(17157..17807)
                     /locus_tag="ACHAUG_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8480109.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACHAUG_001002"
     CDS             18011..18307
                     /locus_tag="ACHAUG_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8480110.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACHAUG_001003"
     CDS             18479..19099
                     /locus_tag="ACHAUG_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8480111.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACHAUG_001004"
     CDS             complement(19191..20357)
                     /locus_tag="ACHAUG_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8480112.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACHAUG_001005"
     CDS             complement(20369..20722)
                     /locus_tag="ACHAUG_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8480113.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACHAUG_001006"
     CDS             20850..21872
                     /locus_tag="ACHAUG_001006"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8480114.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACHAUG_001007"
     CDS             21891..22052
                     /locus_tag="ACHAUG_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480115.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACHAUG_001008"
     CDS             complement(22311..22745)
                     /locus_tag="ACHAUG_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480116.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACHAUG_001009"
     CDS             complement(22855..24714)
                     /locus_tag="ACHAUG_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8480117.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACHAUG_001010"
     CDS             24715..24855
                     /locus_tag="ACHAUG_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8480118.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACHAUG_001011"
     CDS             complement(24832..25992)
                     /locus_tag="ACHAUG_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8480119.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHAUG_001012"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHAUG_001012"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8480120.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACHAUG_001013"
     CDS             complement(27739..28437)
                     /locus_tag="ACHAUG_001013"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8480121.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACHAUG_001014"
     CDS             complement(28394..30055)
                     /locus_tag="ACHAUG_001014"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8480122.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACHAUG_001015"
     CDS             30273..31673
                     /locus_tag="ACHAUG_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8480123.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACHAUG_001016"
     CDS             complement(31925..33004)
                     /locus_tag="ACHAUG_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8480124.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACHAUG_001017"
     CDS             complement(33090..34049)
                     /locus_tag="ACHAUG_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8480125.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACHAUG_001018"
     CDS             complement(34061..35572)
                     /locus_tag="ACHAUG_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8480126.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACHAUG_001019"
     CDS             complement(35594..36157)
                     /locus_tag="ACHAUG_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8480127.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACHAUG_001020"
     CDS             complement(36163..37026)
                     /locus_tag="ACHAUG_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480128.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACHAUG_001021"
     CDS             complement(37040..37936)
                     /locus_tag="ACHAUG_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480129.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACHAUG_001022"
     CDS             complement(38111..39400)
                     /locus_tag="ACHAUG_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8480130.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACHAUG_001023"
     CDS             complement(39372..40889)
                     /locus_tag="ACHAUG_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8480131.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11821 a         8589 c         7096 g        13495 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgcc ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgt
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt a
//
LOCUS       DAYYBH010000016        38296 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000016 DAYYBH010000000
VERSION     DAYYBH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.016"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(116..265)
                     /locus_tag="ACHAUG_001024"
     CDS             complement(116..265)
                     /locus_tag="ACHAUG_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480132.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1233..1559
                     /locus_tag="ACHAUG_001025"
     CDS             1233..1559
                     /locus_tag="ACHAUG_001025"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8480133.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1567..2475
                     /locus_tag="ACHAUG_001026"
     CDS             1567..2475
                     /locus_tag="ACHAUG_001026"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8480134.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3101..3355
                     /locus_tag="ACHAUG_001027"
     CDS             3101..3355
                     /locus_tag="ACHAUG_001027"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFM8480135.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3897..4055
                     /locus_tag="ACHAUG_001028"
     CDS             3897..4055
                     /locus_tag="ACHAUG_001028"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480136.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4127..5352
                     /locus_tag="ACHAUG_001029"
                     /pseudo
     CDS             4127..5352
                     /locus_tag="ACHAUG_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5441..5833)
                     /gene="rpsI"
                     /locus_tag="ACHAUG_001030"
     CDS             complement(5441..5833)
                     /gene="rpsI"
                     /locus_tag="ACHAUG_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8480137.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5849..6277)
                     /gene="rplM"
                     /locus_tag="ACHAUG_001031"
     CDS             complement(5849..6277)
                     /gene="rplM"
                     /locus_tag="ACHAUG_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8480138.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6811..7278)
                     /locus_tag="ACHAUG_001032"
     CDS             complement(6811..7278)
                     /locus_tag="ACHAUG_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8480139.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7398..8936)
                     /locus_tag="ACHAUG_001033"
     CDS             complement(7398..8936)
                     /locus_tag="ACHAUG_001033"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8480140.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9016..9702
                     /locus_tag="ACHAUG_001034"
     CDS             9016..9702
                     /locus_tag="ACHAUG_001034"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8480141.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9903..10430)
                     /gene="msrA"
                     /locus_tag="ACHAUG_001035"
     CDS             complement(9903..10430)
                     /gene="msrA"
                     /locus_tag="ACHAUG_001035"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8480142.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10514..11191)
                     /gene="pgmB"
                     /locus_tag="ACHAUG_001036"
     CDS             complement(10514..11191)
                     /gene="pgmB"
                     /locus_tag="ACHAUG_001036"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8480143.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11188..12316)
                     /locus_tag="ACHAUG_001037"
                     /pseudo
     CDS             complement(11188..12316)
                     /locus_tag="ACHAUG_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(12316..14499)
                     /locus_tag="ACHAUG_001038"
     CDS             complement(12316..14499)
                     /locus_tag="ACHAUG_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8480144.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14496..15521)
                     /locus_tag="ACHAUG_001039"
     CDS             complement(14496..15521)
                     /locus_tag="ACHAUG_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8480145.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15566..16393)
                     /locus_tag="ACHAUG_001040"
     CDS             complement(15566..16393)
                     /locus_tag="ACHAUG_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8480146.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16390..17457)
                     /locus_tag="ACHAUG_001041"
     CDS             complement(16390..17457)
                     /locus_tag="ACHAUG_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8480147.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17483..18319)
                     /locus_tag="ACHAUG_001042"
     CDS             complement(17483..18319)
                     /locus_tag="ACHAUG_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480148.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18316..19185)
                     /locus_tag="ACHAUG_001043"
     CDS             complement(18316..19185)
                     /locus_tag="ACHAUG_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480149.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19255..20538)
                     /locus_tag="ACHAUG_001044"
     CDS             complement(19255..20538)
                     /locus_tag="ACHAUG_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8480150.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20552..22237)
                     /locus_tag="ACHAUG_001045"
     CDS             complement(20552..22237)
                     /locus_tag="ACHAUG_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8480151.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22512..23537
                     /locus_tag="ACHAUG_001046"
     CDS             22512..23537
                     /locus_tag="ACHAUG_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8480152.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24132..27785)
                     /gene="rpoC"
                     /locus_tag="ACHAUG_001047"
     CDS             complement(24132..27785)
                     /gene="rpoC"
                     /locus_tag="ACHAUG_001047"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8480153.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(27864..31481)
                     /gene="rpoB"
                     /locus_tag="ACHAUG_001048"
     CDS             complement(27864..31481)
                     /gene="rpoB"
                     /locus_tag="ACHAUG_001048"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8480154.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32209..33177
                     /locus_tag="ACHAUG_001049"
     CDS             32209..33177
                     /locus_tag="ACHAUG_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8480155.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33195..34100
                     /locus_tag="ACHAUG_001050"
     CDS             33195..34100
                     /locus_tag="ACHAUG_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480156.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34097..34903
                     /locus_tag="ACHAUG_001051"
     CDS             34097..34903
                     /locus_tag="ACHAUG_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480157.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            35060..36028
                     /locus_tag="ACHAUG_001052"
     CDS             35060..36028
                     /locus_tag="ACHAUG_001052"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8480158.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(36345..36869)
                     /locus_tag="ACHAUG_001053"
     CDS             complement(36345..36869)
                     /locus_tag="ACHAUG_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8480159.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            37156..>38296
                     /gene="scm"
                     /locus_tag="ACHAUG_001054"
     CDS             37156..>38296
                     /gene="scm"
                     /locus_tag="ACHAUG_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8480160.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDST"
BASE COUNT        11048 a         8076 c         7114 g        12058 t
ORIGIN      
        1 aaataataca acttagtcat tctttacaac gatttataga aaaacaacct ctttaaatat
       61 aaaaggtacg ggttactgat tacttgtaaa tctttacgtt ccagttttat tctttctaaa
      121 aaatatactc acttcttttc ttagacttct ttttttgatt ctcctcttta tggtcttgtc
      181 cttttttgcc ttcaaaatat ttaaataaac ccctttcaaa agcatttcta ggttttggac
      241 aataaggaat gacttttttc ttcatataaa tacctcattt aactatttga tatcatcaca
      301 ttatcaaaaa aaataggttt tttattaaaa ctatcttgac ctttcgggag atttcactgt
      361 gatttatttt tattcccata cttttttcgt tggtcacttt cgtataccta atctaagcga
      421 tcatcggcga cggtgctttt acaacgaatg gcaggacaaa acaaggggcg gaaaaacttt
      481 tttgcctatt tcataaaaaa ctttttccta cagcaatccc ttgttttgtc ctaccacacg
      541 ttgtgaaccc aaagcacgcc gacgaacgct tagctatacg aaaatgacct ccaaaaacaa
      601 aaaagtgagg tgaatagaaa caaaaagaaa ttgaaatctc ccaaaatgtc gtccgcctcg
      661 gagacaactt gctgctgaaa atggttggtt aattcattta agtattaaat aagataaatc
      721 atcaaattaa tcggaaaaat agcatgattg gccttatcaa aaataaactg taaaaactta
      781 tatttctatg agtggtggaa caatgctaat accaaattaa tttatcgatt acccacctgc
      841 acaaaccaaa gatagcatta ttaggaaacg aatcaatcag cgaatccagc attataatga
      901 ggatactaaa aataaagttt tcataattat aaatccgcct tcctacgaaa ggcaccaatg
      961 aggtgatgaa aaccttagag cgctttcttt ctttttttat tccgtgttac cctttccttg
     1021 caggtccctg gtacactaca gaacccgagg aggtgagtgg cggggctgta tagcggcaac
     1081 cccgcataat tatatggtgt cggtcatccg ttaaggcatc aatgaatggc gcaaacccgt
     1141 agcccgtaaa cttatctctt ccgaagttga ctcgatttcg ccggatgacc agtccctacc
     1201 aaacctgtac tattttttta cagaaggttg ctatgttcaa aatctttcgt aaaaattgtt
     1261 gtggcttgga tgtccacaaa acgtggatct atgcgtgtat cggaatcaca gatgcgaatg
     1321 gtcgtacagt gtataagcaa gctcgcttct cttcctttac aaaaggtctc aatgaattag
     1381 ctgaatggct tgctaaatac aactgtaagg atgtgtgttt ggaatcttcc ggtaaatttt
     1441 ggattcctgt ttttaatatt cttgaaaaaa cttgttttgt cacactggct catccgaagt
     1501 acaccaaacc ccagaaagga aataaaacag atcgcaaaga tgcaaaaatg gatttgtgat
     1561 ctctttatgt gcgacatgat caaacccagt tttattcctg gtcttgaaat ccgccagctt
     1621 cgtgatctaa tgcgttatcg gacgaaactg accaacatga aaacaagtga aaagaatcgt
     1681 gctttaaact gcttaaccgt ttctaatctg aaactagatg atgtttttag tgatgtgttc
     1741 ggaaaatcat ccacttctat catccaatac ttgttagaac atcccggtga atcatttgat
     1801 gttcgaccat ttatccatgc ccgttgtaaa actcccgttg aagaaatcca agcggcagtt
     1861 gagggggcga tttctgtacc tcaagcgatt aagcttcgcc aatgcctcaa ccatattaac
     1921 gagattgaga agcatcgaga agagatcaat ctagagattc aagctcttgc agaacccttc
     1981 tcccctgttt tggaactact tcgtaccata ccgggcttag acactcagcc aataacagcg
     2041 attgcgattc tttcagaaat aggaccggat atgtccgtgt ttccaacttc gaaacacttt
     2101 atttcttggg ctggatgctg tccaagaaac gatcgcagtg cccgacgaac aaaatcaaca
     2161 cgtatctctc gtgcagggca gtcgttgaaa ccgctacttg tacagattgc caatgctctt
     2221 ctcagatcca aagaacatcc agaatttaaa aatcgctatc gtagaatcaa agcgaatcgt
     2281 ggacacaaga aagctatcat tgctatttgt aagatgctcc tgaccgctat ctggaatgtc
     2341 ttatcaaagg tggaacccta ttctgctaaa ggatatctgg agccaaaacc tgcgaaagaa
     2401 gagaaagttc tgacgcaatc ccaagctctg gaccttttaa gaaaacgcgg atataccatt
     2461 atagatagtg cttaaaccaa agttacgttg attcttccta aatagactcg ttgtcttcca
     2521 tttcaaaaat tttgaaaacg gtaggcttat ttagtgtacc gttcttcagg tatcttcaaa
     2581 tagatttatt ttcaaactta tttcctttct tcttttactc tcctatagct tagcatttaa
     2641 gtgggcactc gtgtcacctt gctagatagc tatgaatgcc gtaataagcc tcctagtata
     2701 taggcagact gccaattata actgattcaa tccacattat gataaaatcc accttttcgt
     2761 ttcatatgtg ttgaaatatc acatatgtca aaagtgcacg agtcttttca ttcagtgctt
     2821 tgtaaatact caaaattatt ccttttcact ttaaaaaaaa gataattagt acaaaatcag
     2881 aaatgaggaa agtattagga tggactagaa aggagttgtt gaatttgttg caacagattg
     2941 gtcatttatc aattaaaaat taggtgaagt tttattcatc actgcagctg ctgtttccca
     3001 gagaataaaa cgtctagaag aaactggtca gttactgtat tatcaagcag caattgacta
     3061 cgaacaagct tgattactaa ccaaagcgtt tatccacctg ttgattaaat caaatcaaaa
     3121 atcaggtttt tatacgttca taaaagcaat ctccagtgta ctcgaatgtg actgtatcat
     3181 aggtccttat tcgatgttaa tcaaaatggg gtttccttct accgaatcat tggacagttt
     3241 tatcaatgat ttatcaaagt ttggacgaac caacacccaa atcgttttct cgactcttgt
     3301 ttccttaatg gtttatcagt tctggaatgt ttatcatgcg cttttattaa aatagaaaag
     3361 agcttttttc atgcgacaaa gcagtgatca tttattggca catgcaatct ttgtggatcc
     3421 attttttgaa aatacgatat ttagttttga cccaaaaatc tttaaatacg tatttgataa
     3481 tttatcttaa caaccagata attttattca gcaccgtatt atctatattg aattctcgga
     3541 atcaagataa atttttttcc gtttttgttt cttttttaat cttcttcttg ttattaatag
     3601 tgattaggat gatattccta tattgatctt tgataattag atataatttt tgaataacgt
     3661 tccctgctat tcgagctaga aatacaagta cgccatgacc tagttagcga caactactta
     3721 gagatttctt ttgttctggg acaacaaaat gccatgacaa tagcagtaaa taatgatact
     3781 tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta cccaaagcgg
     3841 ttcaaaaatt ttaaactatt attttaggag atgattttat aaaatcgaaa gcctacttga
     3901 ataaaaaaca agcctgtgaa tttctaggaa ttgataataa tactttagat aagtggattc
     3961 gtgtatataa ttttccagta ataaaaattg atggcgtagt tcgctttgac gtagaggata
     4021 tcaaagattt catgaataca ttcaaaaaaa attgaaattt tcttctcgtc acatatacta
     4081 taaatagcga aatgtactgc cttacttttt aaaggagtga ggaagtttgc ctacaaagct
     4141 ttcaaatggg aaatataaaa ctaatctgag gtatccaaaa aaattcagag aaatcactgg
     4201 aattgcatca gagaagtatc aaaaagtatt tccaactagg caattagcta ttaaggccga
     4261 aagggaaatg aaaaagaaga ttgaaacagt tttaagagag gagaatgcaa actcactaga
     4321 attgaaagga aaaataaaat ttaaagattt ttatgaatca aaatggcttc ctagatatga
     4381 actaggtcaa actactaggt caaacagggc tccaagtaat gtaacaatta gtaatactaa
     4441 ggatatattc cgattgcaca tccttccaat gtttggtgaa tatgcaatga attatctgaa
     4501 ttcaaatact gagataatat cagatgaatt aacaaagaaa tcgaaagaat acgcaaatat
     4561 aaaaattatc aaaggctatg ttcgctctat gtttgatatt gccgaaatac tatgttatat
     4621 cgaattcaac cgaactacca aaattattca atgtattacc gcgcctaaga aaatggcttt
     4681 agaagaaaaa aggataaggg aagggaatca agcattatca tcagaagagc tgactgattg
     4741 gatagaagca gtaaacaatg acttaaataa tcacttgctt acacttcacg attacacact
     4801 tttcatgctt accttatatc taggtgatag aaaaagtgag acttatgctc tgcaatggaa
     4861 atatattgat tttaataaaa atacggttcg tctcaaatat gctttagata aatatcaaag
     4921 aaagacacat acaaaaggtt ggaaagacac cgttattcag gcaccagaag ttgtgatgac
     4981 attattaaga gaatggaaat cagctcaggc tgaacaattg ttgaaactaa aaattataca
     5041 aacacctgac cagtacctct ttacctattc caaaccttct ggagaagtaa attgtcctgt
     5101 tcacgctgac tacttaaact atcgaatcaa tgcaataaaa aaaggcatcc tgaattggct
     5161 caattatctc cacacaaact tcgtcatacc tatgcaacta tagctaggga aggtggagca
     5221 aatatgaatc aaatctctaa tgctttaacg cactcggata tttctactac aaaaatatac
     5281 gtgaacacac ctgacgtagt tgataaatct gtttttgaag catttcaaaa aggattgaat
     5341 aagtgtgatt aacatgtgtt ctcaaatgtg ttcttattat aaaaaaagac actttccaaa
     5401 taattgaaaa gtgtcctgaa atattgatat aaaaagaata ttaacgtttt gagaattgtg
     5461 aagctttacg agctttttta agacctggtt ttttacgttc gaccatacgt gcgtcacgag
     5521 taagtaagcc agcacgttta agagctaaac ggaagtcagg atcaacttct agcaatgcac
     5581 gagcgatacc atgacggata gctcctgatt gtccagcgta tcctccgccg tttacgttaa
     5641 caaatacgtc atatgcgcct tttgtttcag taacagcgaa aggttgattg attacttcac
     5701 gcaagtcagc atgtggaatg tattcttcaa catctttttt atttacagta attttgccag
     5761 ttcctggtac tagacgtacg cgagcaactg cgtttttacg acggcctgtg ccgatatatt
     5821 gaacttgtgc caatgaaatt ccctcctatt agattaagtt tgtgatgtct aaaacttctg
     5881 gttgttgtgc tgcatgtgga tgttctgctc caccgtatac gttcaatttt gtaaattgtt
     5941 tgcggcctaa agtgttttta ggaagcatac cttttacaga agtttcgatc aaacgacgag
     6001 aatttttagc acgcaattcg ccagcagtga ttgatttcaa tcctcctggg tagttgctgt
     6061 gacggtaata aattttgtca gtcgcttttt tacctgttaa tttcactttg tctgcattga
     6121 tgacgatgac gaaatcgcca gtatccaaat gaggtgtgaa agttggttta tttttaccac
     6181 gtagtacaga tgcaacaact gttgaaagac gtcccaatgg aacatcagtt gcgtctacta
     6241 cataccattt acgttctact tcgcctggtt tggccatata tgttgtacgc acgttatttt
     6301 cctccaattt aagttctgat gtttgacata cacttcgagt ttccggggct ctttgtgggg
     6361 caaacaatac catttaacat attacccgct aatctagtca atgtcaacgt ttttctgctt
     6421 gattgaaaaa tttatatgaa actttacttc ttttctttct tttatataaa agggtcgttt
     6481 cctgtcaaaa tgaaaaacaa aaagaggctg tgacaaaagt cttgtcacaa ccccttattt
     6541 tgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc cataaaacat
     6601 aagaagctgt tttttaaaat tgtttcttgt gactcggagc tgaacggcag gcttacaacc
     6661 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
     6721 tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg atttcacaac
     6781 ctctttttcg gtcaagttca acaacagttt ttacgcgtcg atctctggcg ctttgcccaa
     6841 atgagcttgg atcatccaga tacgtttttc tgtggctgtt ttgaattcga taaagatatc
     6901 ttgtgttgga tcgtcgcctt cttctccaga aacttcgatt cctttttggt aaagatctgc
     6961 tagataacga tagcctgcaa caagtgtttc caaacgttct ggaatagtac gatcccagcg
     7021 gccaatttca tcagggattt ttgtgttatc tgcaaattca cgcaatgtag aataaggtga
     7081 accgtctaat gtgatcaagc gttcggatac ttcatctaac tgagcgttca aatcatccat
     7141 gaactcatcc atcattgggt gcaatgtaag gaaacctggt cctctcatgt accaatgtgt
     7201 ttggtgaaca acaacagaca tttggcttag atctgcaact aattgattta atacttcttt
     7261 ggtttgttgg aatttcatct ttcttgcctc ctaacatttt cttacactta aaataatagt
     7321 agactttcaa tgaaaagtac aacaaaatca tttacctgag aacgattttc agttcgcttt
     7381 tttatccgta aagccaatca tttttcagaa tcagctaatt cttctcgcag ttgagcaaat
     7441 tgctgataag tctgtgctaa atgcttttgt gtcgaaacaa ataatggata gattttctga
     7501 tattttttat gatcagtttc ttgtactcca acttgctctt tcaattctat caattcctgt
     7561 gcttcactga tactttggat ctttccgatg cttctccagc ctaaaatcac agctcctaat
     7621 gcggatgctt ctgtgacagt agggaaaaca agcgggtaac ccagtacatc agccatcatc
     7681 tgacgaaaaa tcggagattg agcaaacccg cctgtagcac ggatctctgt gagctgattt
     7741 tcctggcaaa ctccttttaa aatccgataa aggttgagac agatcccttc tagaatcgcc
     7801 ttcatcattt ctttttctgt atgttgccgc tgtaaaccga taaaactgcc aaacgcatct
     7861 gcgtcccaaa gcggtgctcg ctcaccaagt aaatagggat gaaaaatcag accattggca
     7921 cccgctggca caaaagcgat agccgaaagc agtgcatcat atggattttc tcctttttct
     7981 ttagcagatt gaacgacttc ctgtaaaaat gtatgccctg cccaatcaaa gatgccagca
     8041 ccattgctag tcgctcctcc cactacccaa tgtttctgat ccagaacata acaaaacgtc
     8101 tccatttctg gatggaactg aggctgagta gtaataaaac gaagtgctcc gctcgttcct
     8161 acggtcaaag cggcgatcgt atcaccaaaa gcacctaacc ctagattaga aagcggcccg
     8221 tctgcccctc ccaaaacaaa tgatatatca tttggcaaac ctagttctgc tgctgttttt
     8281 gatggtagac ctgttacttg ataggtggaa ggaaagactt gcggcaattg ctctattcga
     8341 atgttcaagt agtcaagcac ttcttctgcc cattggaatt catggatatc aaaatatcca
     8401 gttcctgaag ctgttccgta gtcgctgatc agttcgccaa acaatctata aaagatatag
     8461 tctttgatac cgacaaaata agcagttttt ttgaataatt caggttggct gttttgcagc
     8521 cacttgattt tgttcaatgg tgtcattgga tgaatcggcg tcccagtctg ccgataaaat
     8581 tctttgccaa gcacggtata tttcagtttt tctgcttcgt ccgctgctcg gttatctgcc
     8641 caagtgatca ttcgagataa cggtgttttc tcttcatcca acaatatcag gccatgcatc
     8701 gcagaggaga aggagacaaa ttgaatgatt gtgtctgaag gcaactgttt aattacttct
     8761 tttataacgg tgcagacacc ttgaaagatc aattcctgat cttgttctgc catcccttgt
     8821 gtttcttgga tcaaagggta gcgaaaataa gcggatgctg cttcttgggc ttcatctgtg
     8881 aaagcaactg ctttggtttg tgtggttccg atatctaatc cgattgaaac gttcattttt
     8941 tcacctcaaa ttttatgtat gtacaaaaat ttagtttaag cttgcgtatc ttggttttga
     9001 aaagggggag atggcgtgtt actttatttt atcattggtt gctgtatcgg atcattttta
     9061 tgtttaaccg cccagcggat tccgttaggt cactctatta tttatcctag atctcattgt
     9121 gtcaaatgct gtcgttcgct ttcttggtat gaattgattc cgattctctc gatcatcgtc
     9181 caacgatttc gctgccgcta ttgcaggtgt cggttgccct tctactattt gttggcagaa
     9241 gccttatgtg gtggtctttt tgcctggttc tttacttttt cttccgcaag aaatttttcg
     9301 acgttcatat ggatgctttc ggctttgcta ttttcgttga tggatttatt ttacttcatg
     9361 gtccattctc acacgctatt ctgttcttgg gcaattttat ggattttttg gttgcaggcc
     9421 ggcgtcttcc aatggcaaag tgtgctgtta acgtttatcg taggcggtgt gtgccttcgc
     9481 tatggtcaat cctatctcgg agctggcgat actctgttga tgctttcttg gagtggtggc
     9541 ttgtctctag aagagttgct tcagatcttg tttcttgcta gtctgtttgg aatatccgtt
     9601 ttttctgttt actttatctg tcataaaaaa aagttggaaa agctgccttt cattcctttt
     9661 ttgagcagtg cgttgctgat tgtccttcat cttaggtatt gaaaaaaggt tgtgacaaac
     9721 gacttgtcac aacctctgat atatagtgtt ctcttaccaa atccttgtga cttggagcta
     9781 ggcgacaggt tcacaacctc tccactaaat ggtgttcaat cagctgaccc tcagtattag
     9841 ctcaaaatgc tggttttaca acctctgata tacggtgttc cataagtagc tttttcggct
     9901 tcttatttct tgacgcccca atgtcgacga aggaaacctg cacgtccaga accttctcga
     9961 taaagattgt aatggagctt gtttttcttg taataatcct gatggtaatc ttctgcagga
    10021 taaaattcgc ttcttggttc gatttttgtc acgattggct tatcgaatcg cccgctgtct
    10081 tgcaatgctt gttttgaacg ctctgcaata tttcgttctt cttcatcatt atagtagatc
    10141 actggacgat aagaatcacc gcgatcagcg aactgtccta atgcgtcagt cggatcggtt
    10201 tgctgccaat agatttccac caactgatca taagaaataa cttctggatc atattcgatc
    10261 tctactgctt ctgtatgccc tgttgtgcct gttgttactt gttcataagt tggatttggt
    10321 acatgcccgc cagtataacc ggatgtcaca gaaatgatcc ctggctgctc ttcaaatggt
    10381 ttgaccatac accaaaagca tccgcctgcg aaaactgctt tttttgtcat ataaaatccc
    10441 ccttttttct catcatacct tgatttccaa aaggaggata acgttttgct tgtgacagaa
    10501 aaaacggaaa ctattatata cgtgatacgg cttctgataa atgccaaaac gaacgtactt
    10561 gatcaaaaac ggttaaatca ggaatcacat attcagcaga aggaagattt atgttttcac
    10621 ctattcccaa tacatgcatg ttaccagcca aagctgcttg acaaccagct tctgaatctt
    10681 caatgaccaa gcatgcattt ggcggtacat tcaattgctg tgctcctttg agaaaaactt
    10741 ctggatcagg ttttgctttt gatacttgcg tgccgtcaat aaggacatca aaataacttg
    10801 tcaaattcgt tttttctaaa atgaggcgtg catttttgct tgctgatccc aatccgatct
    10861 tgatgtttgt ttttttcaag tactcaagaa cagctattgc accaggcaaa acggatgttt
    10921 catccatttt ttgaatcgct tgaagatata gattgttttt ttgctcagca aaggctgatt
    10981 tctctttttc tgagaatttc tgttccattt ttccaaatga cagcaatcgc tctagtgact
    11041 cggttctgga tattcctttc agttgctcgt tttctttctc agtgaatgga atggacagct
    11101 ggttagctaa aaccagccac gcttcgtaat gataacgagc tgtgtctaca agaacgccat
    11161 ctaaatcgaa tagtgctgct ttgattttca tcgattacct ccttgaaaat gaacgacttg
    11221 aagtaaagat gcgatgtttt tagctgtgcg atgaagatta tcagtggctt gctgtaaagc
    11281 ttcttgtaaa ggcacagctg caggaaggat cggaaaaacg gccgaaatgc caacatctgc
    11341 tgctttttcg cttcctttac caacacttcc gcaaatagct atcacaggaa cgccctctgg
    11401 tactcttcta gcaatcccaa ctggcgcttt tccttttaga ctttgtccat ctagacgtcc
    11461 ttcgcctacg attacgaggt ctgcttgctg tacccgtatc gaactccagt aaatccaata
    11521 cgtagtcgat gccagagact attttccctg aagcaaatgc taacagtcct gcacccattc
    11581 caccaccagc actactgccc gatgcctcga ttacttgagg ggcaaatctc atataaaaat
    11641 catgcatggc ttggtcagcc actgctaatt cattttcttt caagcctttt tgtggtccga
    11701 aaacaaaaga agcgccttct tttccgcata aaagattagt aacatcagta atgacagtga
    11761 ttttggcttt aatcagctca ataggcacgt tcgttttatc aactgtagca attttaccta
    11821 gattttctcc cagtgcttct actttttgat tatcttgatc gtaaaaagca taacctagac
    11881 cttctgccat gccgatgccg ccatcgttgg tactacttcc gccaacgccg atgatgatgt
    11941 gtcgaacacc ttgttgcaac aattgtcgta tcagttctcc tactcctcgc gtggataacc
    12001 taagtggctg ttgttggttt agtgggacat ctttcaatcc gacgatttct gccatttcaa
    12061 aaatcgcagt ttgaccgtct gaagcgtact ggatttcttt gtttttgcca aatgcattag
    12121 taacgactgc tgtttgccgc ttcaacctta aactgtctgc taatgatgaa accgttcctt
    12181 ctcctccatc gcctataggt agcagatcat attcagcctg aacaaaaact tcttgaaaac
    12241 ctgattgaat ggcttgtgct gcctctaagg ctgtcagact ttctttgaaa gaatctggcg
    12301 caatcagaat acgcattatt ttcccctccg ttccaagatc agttcttttt ccagctggta
    12361 ttggtctcca ttgacaaaaa taggtaatgt ttctttcgtc tcttttttta ttactatttg
    12421 tttttttgtc aaaacaaata gcaacctgtg atttttccaa ataaaaggaa aagaaatttt
    12481 ttcccatgct gaaggaagtc ttggagaaat agaaagagtt ccattcgtta ctgtaatacc
    12541 tgaaaaacca aatataactg ctagccatag tgctcctaat gaagcagcat gaattccgtc
    12601 atctgatgaa tgcggatctg gtcctaaatc aatcaggcac gcttcttgga ataaatgata
    12661 cgcccattcc atttcttgga tacggcaagc ctctatggcg tgaatcgctt tactcaacga
    12721 tgagtcgtgg attgttttgt tttcatagta atgcaaattc ttagaaacga ttggttgaga
    12781 aaaatactca ggtaataagt ataacagcat gacaagatct gcttgcttgc tgacttggag
    12841 ttcatttact tcgctacggg cataatcaag taaaatagac tggctgccag gacgttcttt
    12901 gtaagtggtc aaatcaataa ctggcttact gagatacgtg tcatcttgcg gaatcagctg
    12961 ttctttgtta ggcactggca aatataactt atctaaaaaa ttttgacatt ctttcaaaaa
    13021 aagcggatcg ccagcttgat atttaacagc taaggccaca ttataatgcg ccagatagtt
    13081 cgtatatgca ttattgtcga tatattctgt gtattcatct ggaccaatga ctctgtgtat
    13141 ctctaaatga tcatcgactt ttacagctct gctcagccaa aaagctgctg tttcatgaat
    13201 gagcgttttt ccctttgttt ctaaaaacga ttgatcctct gttgcttgga catactcttg
    13261 aacggcataa gcaaggtctg cactgatatg atgttctgcg gcagctgaag caacttcttg
    13321 tcttgttccg gttttgatat tcaatgcagc aaatttgggt gtctcttcct gaccagtgtg
    13381 tgcgctttcc catgggaaaa gtgcaccttg atagtgataa tctcttgctt tttgccgtgc
    13441 gccttctaat cgatcataac ggtacagcag caaattcttt gctacttcag gaaactgata
    13501 taaaaagaaa ggcaggataa aaatttccgt atcccagaaa acatgccctt tataaccttc
    13561 gccagttagt cccttcgccc ctacactaac acttggatca ttgcggggag tcataatctg
    13621 taaatggtaa gcagcgaagt ccatcgctaa ctgatcgaaa gaagaatcgc cttggatcgt
    13681 tatccgatgc tgatgccaaa aatctgtcca ataagacatc gactgctcta aaatttctgt
    13741 ataactaacg cttttctggg cttgggcaat acttttctta aacgggtctg tttgctgaag
    13801 agaggtagag agcacgatga tcttctccag cgtaaaggac gtattttttc tgatcttttc
    13861 tgaaaaggaa gcggtgatcc ggcgattttt tgcttggaat acacctgctt tattgaattt
    13921 agctgcaatc ccaatatcaa ttttactttc tgttgtctgg taggatgcac tgatcaaatc
    13981 gtcaccaaat actcgcagtt ctttttcaac caactgctgt gtaccaaaat tggtttgctg
    14041 tccatcgata cccgttttca catctacttg gcagtcttcg gtcaacggct tgatgaccac
    14101 tttcagggct gcctgatgga gattattttg atcagcaaca cgacaagtaa tgacttccac
    14161 tttttttcct tgagcagttt caatgagcgc atagcgttta agctctccca tttctaaatc
    14221 tagtgcccgc tcgtatgcaa gaactttcat cgtctcaaga gaaaaatgtt caccgtcgat
    14281 ttttatttcc catctgatga catctgggag attcactaac tcggctgatt ctgctcctaa
    14341 aggtcggtta tatacaccag ctgcaaacat tccccttact tgattccgat agtcttcctc
    14401 ctggcttcct ctgataccaa aaaatccatt tccaatggtc atctgactcg catacttatt
    14461 gatatactct aaatcaaact gtttttcttc tactttcata aggtaataac ttctcctgtt
    14521 tctgccgaac gatataaatt ttccaccaga ttctggatga catatccttg ccagccatct
    14581 gcaatcatct gagtctggtc accgagcgcc cgattcacaa aatgagccat acttagttca
    14641 tgtgggtcat taactggcgt ggtcttttgt aaggaaacca gctttccatc tacatcttcg
    14701 aatagttcgc ctggatacag attagcacct gcctgatcgc cacaaaaagc cacattcagt
    14761 ttgtcttctc tgatattcaa agcaaacgaa gtattcagtt ggatgatctt gccgttttct
    14821 aattccagtg tgccaaaaat tgcatcttct actgtgaatt tgcttggatc ccatttcccg
    14881 aatttccctg aatttttttt cgtaccaatt ttttggaagc tgtacgcccg aacgcttttt
    14941 actttaggaa atccagtaat atacatcgct gtatcaagca tgtgaatgcc ataatcgatc
    15001 aaaggcccgc ctccttgaag ttctttgtcg ataaacgttc cccagcctgg aacaccagac
    15061 cgacgaagcg cattcgcttc tacgtaataa atatccccga gaacatcaag atgattcttg
    15121 atcattgttg cttcgtcaga aaaacgatgc tggaatccat aagccaaaat ctgcccgttt
    15181 ttatcggctg tctctgccat ctttcttgct tgatcagctg tcatagctgg tggtttctcg
    15241 cacatcactg aagcattagc ttcgagggca gccaatacat gttcgaagtg aaagcgattg
    15301 gctgtacaga cactcacgat atcgggagaa atctcattca gcatttgtgt aactgatgta
    15361 tacgcatgag aaaatcctgt acgtcggcaa aagtcttctg ctcgctggct gactggatca
    15421 accacagcgg ccaattctaa gacatctgat cttttttgat aataagcagc atgtactttt
    15481 tctgctactt gtccagcgcc tatgattgct actttcttca tttatttctc tctcctttgc
    15541 ttatttactt attgttattc tttctctatt ctttctctaa atcatttaaa tgacgcgctg
    15601 tttccagaca gtttttcaag tattgcaatg attcctgata aattttcact tcattttctc
    15661 ctcgcaaacg acattcatat accatataac cttcatactc attttgaagt agcgtttgaa
    15721 aatggcgagt aaaatcaatt gcgccagatc ctggttggta gcgatgatta tctgctaaat
    15781 ggatatgacc cacatactct ttttgcgtag tcaatgcttt tgaaatatca tcttcttcaa
    15841 tattcatatg ataaaaatca gcgatgatct gcacatgttt caattgatta gagtcaatgt
    15901 aatgacgcgc atccgctaac aaattgatca tatggtcttg gtatcggttc aaaggctcca
    15961 aataaataac cgtacctgtt tcttcggcta ctttatctaa ataacgaagt gattcgctga
    16021 ctgctttata gtctccttct ttgcttcggg gtgaaaccat cggcggtaat cgataagtga
    16081 acattcccca agctgctggg ataacgattc ctttgcctcc aacctcgctt agtgcacgca
    16141 aaatctcagc aatttgacgt acaccattaa gacgccgttc ttcgataaag tcaccgatcc
    16201 acccatcgta gccgttacaa gctgtcacta ctgggatccc tgtttctttg attgcctgtt
    16261 tgactgtgtc gatattttct attaacaact taccgtcgat ttcgtattct tcaaatccac
    16321 agtctttgat aaatctaaat ttttctaaaa tgtctcttgg gaaaaatgct tgatcttgcg
    16381 taccgaattt cattgttctc ctcctcggac ttgaacgccc attttgatac tttcttctgg
    16441 gtgctggtct acataacgca tgtaagcttc tgcgctttct gcaaatggca caactggatc
    16501 aatgatttct tcacaatcca agtaaccgtt catcaataat tcccaacatg tgtcctcgat
    16561 tcgtttacgg ttccatcttg ggtaatctgg gtttggttca ctagatgctc gagcaaagac
    16621 gatttttgca ttattgaaat gtgcttcgcg accaagatta aacccagctg gaaaaggttt
    16681 agcaaaggca acataagaaa tgatcccgcc atagccgatc cctcttaaag aagcttgtaa
    16741 tgcggcagca ctgccactcg tttcaataac tgaatctact ccacgtttat ctgtcaattc
    16801 ctttagacga aaaccgacat ctgctttcat aggatctagc gtgtaatcgg caccgtattt
    16861 ttctgctaat tctctacgct gcgctatggg atcgatccca acgatggtag tagcacctgc
    16921 ttttttagct aattgaatcg caatttgccc gatagcgcct aaaccgaaga ttgctacagc
    16981 atcacctgct cgaacatgtg cttcccggat accacctaaa gcgaattgag caggatcgta
    17041 acatactgca tttttccatt gatccaaccg atcaagttta cgtagtttat aattatcaac
    17101 accgtttgta atcagcgttt cacgaatggg tccataagtg cagactaact ctcctttttg
    17161 atactctgtc accaaactgc ccgtttcttc gatcgtaccg acaatcatat ttcctaattg
    17221 aaattcgcca aataccacac cgcgttggct tccttcttct cttggcaaga ataggcgcca
    17281 ttcttctgaa aattcttcat cgataaatgg acttaatcct ctaaagtcga caatttctgt
    17341 tcctggtttt ggagtagcga aaattactcg gatacgtact tcgttctttt cgatctcacg
    17401 gtcttcgtaa tctttgaatc cggctattct tggttctaat gcaactaatt ttttcatctt
    17461 ttctttcctc tttcttttct ttttatcctt ttacgccgcc ttcgactaaa tcacctttaa
    17521 taaatcgttc agataacgca tacataatga tgactggtaa agcggtcacc aatgaagcgg
    17581 ccatcattcg tccccaaatg tagtctggtg tactgaataa tgtgttcaaa ccgattggta
    17641 aagtaaattt ttcggaggtc gacaggaaaa tcgaagcaaa taaataatca ttccacgaaa
    17701 ccataaagca ataaacgaag acagaaatga ttccagaaat agccaatggt ataatgatcc
    17761 gaataatgat ctggatccgg ctaagaccat cgatcattgc tgcttcttca atatcagtcg
    17821 gaatcgtgtc aaaataactt tttagcatga atatcgcagt aggaagtgtt tgaacgacca
    17881 tcgtgataat cagtgctgta ttggtatcat acaaacctag accggaaatg attttaaata
    17941 atggaacaac gatcaaaata ccggaaaaca tataaacggt gtaaaagcta gcattgattt
    18001 tcgagcgagc tttgaagcgt agttttgaca aagcgtatgc cccgctgatc ccaatcacta
    18061 ctgcagttac ggaggctatc aaggagatca ctaaactatt tttgaaatac atgatgaatg
    18121 gaaaaatctc agcattgaaa atatcctgat aatgttcaaa agtccattct ttcggaaaaa
    18181 tagtttgtgt cgttgcaatt gcttcttgtg aatccttgaa tgatgtcatc agcatgatca
    18241 aaaatggaaa caaattgaca gctaatagca acaaaatcgt cgcgtaaaat aagattttcc
    18301 tgctcttttt atttttcatt tcaagtccac cttctttcgt gagagcaaga cgaccgctgc
    18361 aataatgatg aacaatagaa tcgatatagc tgctgctttt ccaagatcat tgaacgcaaa
    18421 ggctgtctga tacaaataaa cgcctaagat attgacttta ttcgtcagta gatagacatc
    18481 tgtaaacata tagaacatcc agatagatcg taaaacaatc acagttgcta gtactggttt
    18541 gattgccggc agtgtcacga tcttgaactt ctgccaagca tttgcaccgt ccatttcagc
    18601 tgcttcataa agcgttttgt cgactgtttg caaaatagcc aaaaatgaaa taaacgcata
    18661 cggatagtat ctccagatag caaacaaaac gaccataaag aagctagcaa ctggctggtc
    18721 gaaccacagc ggtgcttcgt tgaaaagctg caacctatct actaacagat cattgacgat
    18781 tccataacta ttattgaaca tatatttcca agcaaaaatc aaagaaatag atggcgtaac
    18841 ataacttaat aaaatcagtg aacgcaccgt tttcctaaat ctgaacttgc ggttaagcaa
    18901 aatagctacg gctaatccta aagctgtact gcctgctaca actaagaatg tgtaagcaac
    18961 tgttaaacct aaagaacgat aaaattcact atcacttaaa atttcaagat aattgcttaa
    19021 tccgataaaa acggcgtctg agttaggatt cagcggcata tcgaaaaaac tgatctgaat
    19081 attggaaagc attggaatca gcactaaaac agctaataaa agcatgcttg gtgctaaaag
    19141 tcccatggct aattttaagt cagaatgttt ccttggacga ttcattgaaa cctccccctg
    19201 ataatctgcc aatagctatc tacttttttc gaacaccatt ggcagaactt cttgttattt
    19261 cgtatttttt tgtgcgtcag ctaaagcttg atcgacatct tcgtttccga cagttacttg
    19321 gttcactgct tttccgatca cacctgagct tgtgatatct cccattttcg tgaaattctt
    19381 ttccccgact agtccaaaga cttgtacttc atcaaaagca ctagcaatct cagatggcag
    19441 ctcgccaaaa gacttgatga cttcattttc tttgtaacca tccagttcga ctacttgttt
    19501 attcactggt tgagcaccac caggagacat caagacccac ttttccatgt ttttcggttg
    19561 agataagaaa gctgtgaatg cttcagcagc ctctttttgt ttctcgtcta gaccagaaga
    19621 aatcgtcaat ccagatactg tgccatagac agcttgatct ttttgtgttg ggatagcaaa
    19681 accgatattt tctgagttcc ccgcttcata aacagaaggc aaaatgtaag ttgaatagat
    19741 tgccattggt gctgttccat tcatgaaagc atctttgatt tctgtcacat cattagagcc
    19801 tggcatcgtg tattgggata agttttgata aaacgacaat gcttctctca tcttttccgt
    19861 atcgatcgtt acctcgcctt catcatccaa aacatttgct ccatttgaca aagcaaattg
    19921 agagaacgct tgctcactca ttgttccttc agcagtaggc atagcaattc catattgttt
    19981 attctcttta tctgtgaaat gttgagcaat ttttaaaata tcatcccaat tttctggttc
    20041 tgaaaaacca gcttctgata atttttcttt gtcataccaa ataccttgaa cccagccgct
    20101 aattggtgca gcaatatagc tttttccatc ttctgaacga acaaggtttt tggctccgtc
    20161 ataataatta tcttctccag cttcctcgat aacgttttgg actgccgtct gatcaatcag
    20221 ttcatcttta tccatcactt tagcaaaatc ctgactgact tccattactt ctggtaattt
    20281 accggaacga gctaacgtaa cgatttttgt attgaacgct gattcttcta ccggcacttg
    20341 cttgattttg atgtctggat tttctttttc aaaatcagca accagttcat tgatcacact
    20401 taatcgttcc tgttcgacag aagagtgcat gaattgaatc gttgtctgtt gtgattcgtt
    20461 agagctttga ctttcaccac aagcagtaag aagaaatcca gtagctagca gacaggtagt
    20521 aacaataatt tttttcattt ttttccacct cttattttat tattttcagc cacatatatt
    20581 catacggctg gaatatcaac tttcgttctt gactgcaatg gataatttgt ccagaaaaca
    20641 ggtctaggta atctcctttt ggtaaaagat aacctgctga ttgattggaa aaattatgga
    20701 taactaagac tttctcttcc ttcgcttcgc gaataaaact gataaaagaa tctgctgagt
    20761 ccagtaagta aatcaaacta ttcggatgga aggctttttc tttttttcga atagatagca
    20821 tctgggtcaa ttcaccatat actgctgttc ttaatggatc tgtctgcaac tctttctcaa
    20881 tatctgctaa caaaaatttt tgccgattga tttttcgatt ctctcctgtt ttttctacgc
    20941 ctttgtaatc atttcgactt cccagcacac tttgaatata aattgccggc acgcctggta
    21001 acgttaccaa aaatgaatga gcagctaaaa agcgtttgct tctccattcc gtcgtatctg
    21061 ttggagaact taacgcatct agatacgtag cgtttatctc atatggactg gtcgttccat
    21121 ctgggttttt cttgtagttt actcgggcac cctctttctt cagctgttcc actaattcaa
    21181 ggatcgtttt ttccggcaga atacctcgta ccggattcaa tccgataccg tcatgggagg
    21241 ctaaaaaatt aaaaaaagtt gtttttttcg atgcgaaatc gatggttttt aaccattctt
    21301 tcaacttact tgtattaccc gaacgaatcg cttgaagggt caaaggtggt agcgaaaact
    21361 gatagaccat ctgtgcttca ttctcgccat tcccaaaata actgatatta tctttgtgtg
    21421 gcacatttgt ctcagtgatc aataccgttc ctggagctgc ttcatcagcg attgcacgga
    21481 ataattggat gattttatgc gtttctggca ggtgtataca agaagttcct agttctttcc
    21541 ataaaaatcc cacagcgtcc aagcgaataa atgaagcgcc tttttctaag taaaacaaaa
    21601 gaatatcgat cattttcaat aaaacttctg gatttttaaa attcaagtct acttgatctt
    21661 cgctaaaagt cgtccatacg tgtttttctt tcccgttttt gtcaatgaac gttgtaagta
    21721 acgggctggt ccttggtcta gtcacgctgc ttgtatctgt atccagtggc aaatcaataa
    21781 aaaaatcttt gtacgtatca ttcaagttta aatattcctg aaaccaagta ctttcggcag
    21841 acatatgatt gcagacaaag tcgaacatta accgtgtttc gcttctcatt tcttctatat
    21901 cttcccatgt gcccaaacgg tcgttgattg cctgataatt tacaacggag aaaccatcat
    21961 cagatgtata tgggaaaaat ggcaagaaat gtacaactct aaatgtatct gccaaatact
    22021 tttgataaaa tttcttaaat gtacgcaaag ttggttgctg ctcttcaaaa aattgatccc
    22081 cataagtaat caagaaaata tcttcttcgt cccaatgcag tttgcctgcc tggcctttct
    22141 tctgctgata aacactgact ttttgttcaa tcatagaggc ggttttctca gacaaatctg
    22201 agtcgcgata aatcgcttgt aaaagttttg tatacatcgg attccccttt cttttttggg
    22261 agcgttccca acgatttgat catattatgg aaaacgctta ctgtcaatag cattttttta
    22321 aataattttg atatacacaa aaatacaaat aagaaagctt atgtatcaat aattaatagc
    22381 ttcttaaaaa aatatggtct ctcatgatca tgcaagctat tcctaaattt tcaaaatcat
    22441 agacaaaaaa gaatgtctat tgtactatgg aattattcta aaacgctccc aaaaaggagg
    22501 tgcatataaa attgaaacct actatttatg acattgcgaa aaaagctaat gtctcaaaat
    22561 caactgtctc cagagtatta aataaccagc caaatatctc tgataaagca aaagcaagtg
    22621 tacttcaagc aatcaaagaa ttgaattatg aaccgagcaa aattgcaaga ggactgtcag
    22681 gaaatggttt tgacgcgatt ctagcattat ctcatcgtac ttctcatacg acctatggca
    22741 atcctttttt ttcagatgtt attcaatcca tttctgccgt ttctgaacaa aataacttcg
    22801 acttgattct acaaacggca aaaacaccag aagaagagct agaaaaatgc gcgaaaaaga
    22861 tccaagaaaa aatgatccga ggaattatta ttttaagctc tacgatggat gaaacacttc
    22921 tttttgaatt ggatcaatac aatattccta ttgtggtaat cggaaaagtc ggaaacgact
    22981 acacgaacat ctactcagtg gacacggata atttccagga tagttacgat ttggtggaaa
    23041 cactgatcaa gaatggacac cgtcatatcg gctgtctcca tgctccgaca gaagtttatg
    23101 tatccaatga tcgtctcaat ggttaccgtc aatgcttatt tgatcaccac ctagatatcc
    23161 gtaatgaatg gattatagac ggcggctttc gcttagaaga tagcatggca gcaatgaatg
    23221 atttaatgaa gctacgagaa agaccaactg cgattttttc taccgatgca ttaaaatcat
    23281 tgagtattta caaagtggca gaacaatctg gaaagcagat acctgatgat ctttcgatcg
    23341 caggattcag tgatccctta ttgtcctccc tattttcgcc acagttgact cggatagaca
    23401 ttccgacaaa tcagttagga atcaaagcca cagaactttt attccaacta atccaccacc
    23461 aataccctga agaaaaaaga atcattcttt caaccgagat tactctcaca gattcgatcc
    23521 aacaacaaga aatgtagaac agaattataa aagaacagaa acactgagga taagtcagtt
    23581 actctgttct tttttcaatc actcatccct cgttaccccc tcccggtatt ttagcgaatt
    23641 tttcttattt tccatctgtt ttagatagct gtggataact ataatgtttg tgttaaacta
    23701 ttttttccac agggaagaaa aaactatttt taaccacgtt gtgtataagt ttttatagtt
    23761 cgttttttta aacacattta cccttatttc aatccatgtt atttggggat acttatctct
    23821 aaaacataaa tagaataaca gacatgtcta ctggttttgt tttattcgag gaattattca
    23881 ataatagcta gcaaaatatt aaaaataacc gctctatctt cccatcaatt tattttgcat
    23941 tcaaaaaatg atctatcttt acttttgttg attgacttct cttggaagat gatcgttccg
    24001 ataccttaat tggatacgaa ccgatgttgt taagatcctt tcccactttt cttctagtta
    24061 gacaaattat tttagacaaa ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt
    24121 ttttaaaata tctattttgt ttgattgttc attgcgtctt ctgcagccat ttgcgcttca
    24181 atatctgaaa tactgtagac attttcgctt gctacaccga cttcttttgg ttccatgtca
    24241 cgataacgtt gcataccagt accagctggg atgatcttac ctataataac attttctttc
    24301 aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt
    24361 tcttggaatg atgcagcaga taagaaacta tttgtttcta atgatgcttt tgtgatacct
    24421 agtaagacag gtcgagaagt tgcaggaacg cctcctgaga ctagcgtatt gtagttttga
    24481 tctttaaagt ctgcgatgtc taataatgta cctggtaaga tatctgtatc acctggatcc
    24541 attacacgaa ctttacgcag catttgacga accattactt cgatatgctt gtcgccgatt
    24601 tctacccctt gcatacggta aacacgttgt acttcacgaa gcaagtagtt ttcaacagat
    24661 aatacatcgc gaacttgcaa caattgtttt ggatcgattg atccttctgt caatggtgca
    24721 ccacggtgga tcatatcgcc ttctgctact ttcatacgtg ctgtataagg aacagtatac
    24781 gtacgtgtat ctgtttttcc tttgattgtt acttctttgg tacgtgttgc aggatcttct
    24841 gagatatcga tgacatcccc tgttacttct gtgatcactg cttgcccttt cggattacgt
    24901 gcttcaaaga tttcttggac acgaggaaga ccttgagtga tatcgtctcc ggcaacccca
    24961 cctgtatgga atgtacgcat cgtcaactga gtaccaggtt caccgattga ttgagcggcg
    25021 atcgtaccaa ctgcttctcc gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa
    25081 caatgtttgc agacaccatg ttttgtatta catgtgaaga cagaacggat tgtcactgtt
    25141 tcgacacctg catcaacgat ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca
    25201 atgatcaatt catttgtttc aggatgacgt acttctttac gggtgtaacg tcccactaaa
    25261 cgatcttcta atgattcgat gatttcattt ccctcgcgga tggcttggat ttcaagacct
    25321 cggtcagtac cgcaatcttc ttcacggatg attacatctt gggccacgtc aaccagacga
    25381 cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat cggtcatccc tttacgagcc
    25441 ccgtgagttg agataaacat ttccaagacg gatagtcctt cacggaagtt cgaaatgatt
    25501 ggcaattcca tgattcgacc attaggagcg gccatcaaac cacgcattcc ggcaagctgt
    25561 gtaaagttgg aaatgttacc acgagctcca gaatcagaca tcatgaagat tggatttttc
    25621 gcttccaaac cttccatcag tttttgttgg atgctgtctt ttgcagcatt ccaaacagca
    25681 atgacacgtt cataacgttc gtcatctgtg atcagaccac gacggaattg ttttgtaata
    25741 gtttcaactt gtttatgggc attttcaatg atttcttgtt tttcattcag tacactgatg
    25801 tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt tgtaccctaa gtctttcata
    25861 cggtcaagca tcttagaagt ttcttttact ttgaatcgtt tgaagacttc tgcgatgatg
    25921 tttcctaagt ttttcttctt gaacggtaat actaattctt gctctttgat atgtgcagga
    25981 atatcagtac cagcttcaac aaagtatttg tccggtgttt ggacggtcaa gttgaagtct
    26041 gttggttcat tcagataagg gaattcagga ggcatgatct cgttgaagat gatcttacca
    26101 actgttgtga tcatcgtacg ttctttttgc cattcagtaa atggtttttc acctaatgag
    26161 ttagggttca caccgatacg tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg
    26221 gcttcgtttt gatctctgaa gaccattcct tcgccttcgc gtccttcttc ttccattgtc
    26281 aagtagtagt tacctagaac catatcttga gaaggagtaa caactggttt accatcttta
    26341 gggttcaaga tgttttgagc agctaacatc aacatacgtg cttctgcttg tgcttcttca
    26401 ttcaatggaa cgtggacagc catttggtct ccatcgaaat cggcattata agcttcacat
    26461 acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata
    26521 ccaagtcggt gaagcgtagg tgcccggttc agcaatactg ggtgttcctt gattacgtct
    26581 tctaaaacat cccatacttc gtcttcttga cgttcgattt tacgtttcgc atttttgatg
    26641 tttgaagcta gttcgcgttg aacaagctca cgcatcacga atggtttgaa taattcaatg
    26701 gccatttctt ttggaagtcc acattggtac atcttcaaga aaggtccaac aacgataacg
    26761 gaacgacctg agtagtcgac acgtttacct agcaagtttt gacggaaacg accttgtttc
    26821 cctttcaaca tatgagaaag agatttcaat gggcggttac ctggtccagt aactggacgt
    26881 ccgcgacgac cgttatcgat caatgcatct actgcttctt gcagcatacg tttttcattt
    26941 tgaacgatga ttccaggtgc attcaagtct aacaaacgtt tcaaacggtt attacggttg
    27001 attacacgac ggtaaagatc attcaagtca cttgttgcaa aacgtccacc ttctaattga
    27061 accattgggc gtaaatctgg tggaataaca gggataacgt ccataaccat ccagcttggt
    27121 tgatttcctg aagcgcggaa tgcttctaaa atatccaaac ggcggattgc acgtgtacgt
    27181 ttttgtcctt gtgcagattt caattcttct ttcaattcgg ctgcttctgc atctagatca
    27241 acattatcaa gtaattgttt aatcgcttct gcacccatag cagcttggaa ttcttgtcca
    27301 tattgttcac gtttttcgcg atattcgcgt tctgtcaaca attgtttttt ctctaaattc
    27361 gtatttcctg gttcgatgac tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt
    27421 gggctcatgt ctaatacaag acccatacgt gaaggaatcc ctttaaagta ccagatatgt
    27481 gaaacaggag ctgctaattc aatatgtccc atgcgttcac ggcgaacttt cgaacgagta
    27541 acttccactc cgcatcgatc acaaacgatt cctttataac ggatacgttt gtattttccg
    27601 caggcacatt cccagtcttt tgtaggacca aaaatccgct cacagaacaa cccttcgcgt
    27661 tcaggtttta aggtacgata gttaatcgtc tcaggttttt ttacttcacc gtaagaccag
    27721 cttctgattt tttcgggaga agccaaacct atttgcatac tttcgaattt atttacatcg
    27781 atcaaaaggg gttccctcca tttcatttaa tggcaaaccg caagacggct gatcttcgac
    27841 cgccttgcag ctcatagacc ggattagtct aaattatctt ctgcggtttc aaagttttgg
    27901 atgacatcgt ctttttcatc ttccacttgt tcagcagctt tcttttctaa ttctttggca
    27961 gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg
    28021 tccatgtcgc gaagttcgat ctctgtttct tcgatatcca agacacgcat gtccagacct
    28081 agtgattgca attctttcac caatacgcgg aatgattccg gtacacctgg ttttggaatt
    28141 ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg
    28201 tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag cttccagtgc ccatacttcc
    28261 atttcaccaa aacgttgtcc accaaattga gctttacctc ccaatggttg ttgtgtaacc
    28321 aatgagtaag gtccgattga acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg
    28381 atcatgtaca tcacaccgac agacacacgt ccatcaaatg gttcacctgt acgtccgtcg
    28441 tacagaacag ttttcgcatc gctggccata ccggcttcac gaactgtttc ccatacgtct
    28501 tcatcacttg caccatcaaa tactggcgtt gctacgtgga tacctaactg acgtgcagcc
    28561 atacccaagt gtaattccaa tacttgcccg atgttcatac gtgaaggcac ccctaaagga
    28621 ttcagcatga tatcgattgg tgttccatca ggtaggaatg gcatatcttc ttccggcata
    28681 atacgggata caaccccttt atttccgtga cgaccggcca ttttatcccc ttcatggatt
    28741 ttacgttttt gaacgatata aacacgaact aacatgttta cgcctggtga caattcatct
    28801 ccagcttcac gagtaaagat tttcacatcg tgaacgatac cgccgccgcc atgtggtaca
    28861 cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa agatcgcatg caatagacgt
    28921 tcttctgcag ataattctgt tacccctttt ggcgtgactt tccctacaag caaatcgcca
    28981 tcttggactt ctgcaccaat acggatgata cccatttcgt ctaaatcttt taatgcgtct
    29041 tccccgacat tagggatttc gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct
    29101 tctgattcat attcttcaat atggatagaa gtgtatacgt catcttttac caaacgacgg
    29161 ctcatgatga tcgcatcttc atagttgtac ccttcccaag tcatgaaacc aactaaaacg
    29221 ttttggccta gagccatttc gccttgttcc atagatggtc cgtctgccag agtatcccct
    29281 ttttcgactt tctcacccaa atgaacgatt ggacgttggt tgtagcttgt tccggagttt
    29341 gaacgacgga attttgttac atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg
    29401 atttcagaag catcaacgta ttcaacgaca ccatcatgtt tacataacaa agcagctcct
    29461 gagtcatgcg ctgatttgta ttccatacca gtaccaaccc atggtgcttg aggattgatc
    29521 aacggcactg cttgacgctg catgttggca cccatcaagg cacggttgga gtcatcgttt
    29581 tccaagaaag gaatacatgc tgtcgcaacg gcaactactt gtttaggtga tacgtccatg
    29641 tagtctactt tatcaataga tacttctaag ttttcacttt gagcacgagc cataacaacg
    29701 tcttgagcaa atgttccatc ttcattcaat gggctgtttg cttgggctac gatgtaatgg
    29761 tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct
    29821 acacgacgat aaggtgtttc gataaatccg aatttattta ctttcgcata actggacaaa
    29881 ctattgatca acccgatgtt tgggccttca ggagtttcga tcggacacat acggccatag
    29941 tgggagtagt gaacgtcacg aacttcataa ccggcgcggt cacgagtcaa tccgccaggt
    30001 cctaaggcga aaagacgacg tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg
    30061 aactgtgaca actgagaaga accaaagaat tctttgatac ttgccacaac tggacggata
    30121 ttgatcaatt gttgaggtgt caatgtttca gtatcttgga tcgacattct ttcacgaacc
    30181 acacgttcca tacgtgctag cccgatacgg aattggtttt gtaaaagttc accgactgaa
    30241 cggatacgac gatttcctaa gtggtcgata tcatcgacat tgccgatacc ttccatcaag
    30301 ttcaagaaat agctcattga agcaatgatg tcggctggac gaaccgtttt tacagctgct
    30361 tctggatagc cgtttccgat cacgtttact tcacgttcag ggtctcttgg tgaaaatact
    30421 ttgatgactt ggacagtcat tggatctgtg accacaccgt cttctgatgg atagtaagtc
    30481 actgaattta ggccattatc aatgaaagga gctagtgttt ccatgacttg gtgagtcaga
    30541 actgttcctt tttcaacgat gatctcgcct gtttctggat caactaatgt ttcagctaat
    30601 gtcagattca atagacgtgt tttcaaatct aattttttgt ttactttgta acgaccaacg
    30661 tttgctaagt cgtaacgttt tggatcgaag aaacgagcat tcagcaagtt acgtgagctg
    30721 tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct
    30781 gttcttgagt cgcttgcgtt tttatgcaag tctttttcga ctgtattgcg taatgtttcg
    30841 ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa atcctaacgc acgaactaat
    30901 acggttaaag gaatcttacg tgtacggtcg atacgtacat aagagatgtc ttttgcatca
    30961 gtttccattt ctagccaagc tccacggtta gggataacag tagaaccgaa tccttctttt
    31021 ccgtttttgt cgacttttcc atggaaatag acacctggtg aacggactaa ctgagaaacg
    31081 attactcgct ctgccccgtt gatgatgaat gttccttgtt ctgtcattaa tgggaaatca
    31141 ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt
    31201 acatgtaatg gtgcagagta gtttgcatca tgcgcacgag cttctgctac tgtatacttt
    31261 ggttctttca attcataatc tacaaattcc aaagatagat tgccattgaa gtcatcgatt
    31321 ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta agaaccattg gtaagagtct
    31381 gtttgaattt cgatcaaatt tggtaattcc aacacttcac tgattcttgc gaaacttcta
    31441 cgttcgcgat gttttccgta ttttactacg tgtccagcca agctcttcac ccctctaaaa
    31501 tgtcattcaa aaagactacc cgatcaattg ttacaataat atttcaaata ttatttttag
    31561 gtaacaacag acattttttg cggcttttag gcaaaaaaaa accaaaaata gaacattctc
    31621 ttgaaacctt acttcgtgag cttctacttt tgtttcctta tattaaagcc gaaacggaca
    31681 atatttcgtt tctgcctttt cttcatcaga tagtttttgc aacttcatat actacactta
    31741 ttttctataa aagtcaacac ttcctggcgt ttcttctgct ttttcgcaac attttcttaa
    31801 tgttttcaat tttatgtttt caattatctg acaattctta attgacagac ctttttatct
    31861 cggttatact ttagaaaatt cattttgtgg aggaacttct atgcgaacca acatcaacca
    31921 aatcgcacca acacaactga ataactttta ctttagctac tttagatagg tttgtattca
    31981 tcatgatgga tacaaacgtt aaacgacagt ttgtatctat gatggctaaa gaactttgta
    32041 ttgcagcaaa aagccacgta gacaaaatgc taagtggctg agcagtgaaa atacaaagta
    32101 aaagtatcaa gcattctact ttttcaattt attttccgac ttttcaatcc atttggacat
    32161 tttgtctgaa tggatttttt gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat
    32221 ataatcatcg gattaggcgc tgccctgcta ctagctttag gaggttgcgc acaaaaagac
    32281 aattccgcag aggcatcaga tggacaaaaa acaattggcg tattgcaact tgtacaacat
    32341 aactcgttag atgctgccta taaaggattc aaagaaggat tagcagaaga agggtataaa
    32401 gaaggcgata atctaacaat cgattatcaa aatgcccaaa acaatcaaga caacctgaaa
    32461 agtatgagtg agaaactcgt caaagaagat cctgatttgc tacttggaat cgccactcca
    32521 gcagcacaat ctttattaaa tgaaacaacc actatcccga tgactgtcac agcggtcaca
    32581 gacttggaac aagcccattt agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc
    32641 acgacagata tggtgccaat cgaaaaacaa atcggcttac tgttaaatat cgtgccagac
    32701 gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa actctaagat ccaagctgat
    32761 ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca aagtactgac agcaaattcc
    32821 acaaatgatg tccaacaagt gacgaaaagt ctagctaaag atgtagatgg aatctatatt
    32881 cctactgata acacgttcgc aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca
    32941 aaaacacctg tcgttgctgg cgcagttgat caagtcaaag acggcggtct agcaacagta
    33001 ggaatcgact acgaaagctt gggcaaacaa acagggatca tggcagcaaa aatcttagac
    33061 ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg atttggaact gtatgtcaat
    33121 aaagacatgg ccaaagcatt aggcatcaat ccagacagca tcaaagaagc tgaataaaac
    33181 acaggaggat tctcatgaca aatttgctct ctcttttctt atcatctgca tcgcaagggg
    33241 tcttatgggc cctacttgcg attggtgtat acgtcacttt ccgtatcttg gacattgccg
    33301 acttgactgc tgaaggaagc tttcctttag gtgcagggat cgcggcagta agtattacaa
    33361 atggctatca tccattgaca gcttgtcttc taggcttttt agggagtgcg gcagcagggc
    33421 ttgtttccgg actgcttcac acaaaattga aaattcccgc ccttcttgca gggatcatca
    33481 ccatgactgg tttatattct gtcacatccc gtgtgatggg tgcgccgaac atctctttat
    33541 tgggtcagcc aacgatcttt cattgggtcg agtcattcgg cataacaaaa gacaacgctg
    33601 tactgatcgt cggtctgctt tttgctttga tcgttgtatg tttacttgtt ttgttcttac
    33661 gtacagaaac aggacttgct attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa
    33721 acgggatcaa cacagataat atgaagatca tcggttatat gatttctaat ggatgcattg
    33781 ctctttctgg ctccttgctt gcgcaaaaca acggctttgc tgatttaaat tctggtattg
    33841 gaacgatcgt aattggactt gcttctatta tcatcgcaga agttttgtta cgtaaccagc
    33901 cactatttct ccgtctcttg actgtcgttt taggtgcggt catttatcgt tttattttag
    33961 cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc
    34021 tagtgatctg tctttctttc ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga
    34081 aaggcggtag tgtcaaatga atgacgtctt aacattggaa aatctccatc atacctttga
    34141 aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt aatctttcct tagatcccgg
    34201 cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa tctactttgc tgaacagcat
    34261 cgccggtaca ttttctgtcg atcagggtca aattttattg aatggaaaaa atatcacgaa
    34321 aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta ttccaagatc ctaaactagg
    34381 gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa
    34441 acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca tttttcaaag accagctggt
    34501 tcggctaaat ttggggttgg aaaatcgttt atcctcagaa atcggtttgc tgtctggagg
    34561 acaacgtcaa gctattacgt tattgatggc cacattacag cgtccagaac tgatcttatt
    34621 agatgaacac actgcagcgc ttgaccctgc tacttccaaa acggtgatgg aattgacaga
    34681 aaagctgatc actgagcaga atctcactgc ttttatggtc actcatgaca tggaagacgc
    34741 gatccgctat gggagtcgac tgatcatgct gcatcaagga aggatcgtcg tagatatcca
    34801 gcaagaggaa aaacagcagc tcacagtgcc ccaattatta gcgttgttca aacaaaacag
    34861 cggcacggca ttaaaagacg atcaagtact tttatcttca taatcagaaa gaggatggga
    34921 caaaaaatga acgcccaccc tcttcttttt tctctttcct tctttcgttt ttgctttatg
    34981 tatcaaccgt tgtttcttcg aaaactattt tgttctctcc ttcttcctga tagaatggat
    35041 gggaaaggag cttgatgcca tgacaacgaa agacaaggta cttcaattat tgaaaaagac
    35101 accagatttt ctttccggag aaaagttggc acagcaactg gaagtttccc ggacaagtat
    35161 ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt tttgagcacg ggcctacagg
    35221 gtatcgttac ctgccctctg acgtactaga tgcaaaagaa atccagcaag agctacggtc
    35281 gtttattcct gatttgaaag tacaaacgat gatgacttct gaatctacga tgaaagatgc
    35341 caaactggca gcagctcaag gagattctgt ttcgaagtta tttgttgctg atacacaaga
    35401 acaagctcgt ggacgttttg gacggccttt tttcgctgaa cctggacgag gaatctacat
    35461 gagtctcttg ctccatccag acaaacggtt tgatgaattg cctcaatata cgatcatcgc
    35521 agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg
    35581 ggtcaatgat atctacatgg atggaaaaaa agtctgtggg attttatctg aagcagtcag
    35641 tgatatggaa agcggcagga tcagtcatat catcattggg atcgggctta atttttctgt
    35701 tcctcaaaat cattttccag ctgatctcca agaaaaagcc atctctgtct ttcccgacgg
    35761 acaagctagt gtcacaagga atcagctaat cagcaaaatc tggcaagaat tgttcactta
    35821 tctttctcac ccagaaaaag cgttggactt ctataaagaa aaatcattcg tattgggaaa
    35881 aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga
    35941 tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg agtttgtctt ctggcgaaat
    36001 cagtctcaca tctatccgta cgcaataatt atcagatttc acaaaaggac acctgatctc
    36061 atacagtgtc cttttttctg aattctcttc aaaagactct ccttattcta tcagctgaca
    36121 gtttctctat cctctagaaa aaggtattct aaaaatatct ataaagcatg gtacactttc
    36181 tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt tacacgatca aaagagatag
    36241 caggccgctt cctttttcaa tcttatagat agaccatggg taacgataaa aacaaaaaag
    36301 aacttgcgac ttattttgcc gtaagttctt ttttagctag tgccttaatc tcttaatccc
    36361 aacaaacgtc tcgcatagat cacctgacca gagtgcatag cagcatcatc aataatagaa
    36421 acgagacgca ttcctcgtgt gactggtggt gtccagttct cgtcaattac ttcatctagc
    36481 tcggaaggtt ccagccttct taagtaagaa atagcaaaat cagcagctcc tcgcaagtat
    36541 cctagcagat cttctatttg attagcttgg atctgctttg cttgttccag actatgcttc
    36601 caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc
    36661 caaattggag aagttcctgc taaatcagca atctggaaat ctaattctcg agcggtatgc
    36721 caagccagcc aagtcattga tttgatctgc ggggctctat cttccactgg aaaacgattg
    36781 gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca
    36841 ctctcgcata gcatatctaa ataatccatt tattttcctc ctttttattt atcctactta
    36901 ctttttccta attctaggat acctacttat atgttactac acacccttat tttttacact
    36961 ttaacagtca ccatcatcaa tagtttggag attgaaagaa aaagatagtg tcgaactatc
    37021 aattgaacaa gaagcaacaa tatgaacgtt tcattaatat tctttaaata aacaatgatt
    37081 tttcaaacga acagtttaag actaggagaa aaagagtata ataggaaaat gtttgaaagg
    37141 aaaggaaggg gaaaaatgaa aaaaagggca agtttactag tcctagttgc tttgttgttc
    37201 aatatctttt cgtatggtgt ggccgtttca gcggaaacat acattcctaa agaccctggg
    37261 aatatttata atgatctaac tggtaactat ggcttgttag gaattgctag tcaattccac
    37321 gtatttgcta aaaataagac aactgtgaat gcacatacag acggaaatat agcaacacat
    37381 gaacttgatg caaaaaataa ttttggtaca gagcttcatg aaggtttatt gaaccaagaa
    37441 ataaattata tacaaaaaac tgattctttc gctaattctt caggcatacc tacaggtagc
    37501 acaagaatga ataaatttgt cgttggcagt aacgtggatg tagcacttca agatggtcat
    37561 cctacagtca atggatccag gcttgatcac ttaactccag atgattttta taaagatcgt
    37621 gctgggcatg aatatattga tttcgagcag gaatttgcta aattgaatgt agcagcaaat
    37681 gatcttgcaa cgatcactcc agctaaaaca tatactgctg cagattttcc agacatgaac
    37741 aatcggacaa tcgatttaac tgatttatca gataccgggt ttttattggt aaatatcgat
    37801 gcagaagtgt taacgatgaa tacgccactt caaatcatta atccaaatga tcaagtagtt
    37861 gtatttaatg tcatcaacag ttcttctgca ttgaacgtac agtcaccaat caaatacaat
    37921 gatcgttcaa atcatgaaac agaagatttt tcggatgcga atatctcttg gaattttgga
    37981 aatgagatga ctgatttgac aatcagtgct ccttttcaag gaacaatcct tgcgcctaat
    38041 gctactatcc gcattactca aaaccaagac ggaacgatta taggaacaaa tgtgatttta
    38101 gatgcagcta ccaatcgttg ggatcctaac gaaatattta tcagcgacaa tggtacagat
    38161 acgacagaca caagcgacag tacagataca tctaccacaa gtgatagctc agattcatca
    38221 acttcaagcg acagtacaga tactacctct accacaagtg atagcacgga tacatcagct
    38281 tcaagcgaca gtacag
//
LOCUS       DAYYBH010000017        36042 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000017 DAYYBH010000000
VERSION     DAYYBH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.017"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACHAUG_001055"
     CDS             <1..288
                     /locus_tag="ACHAUG_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480161.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACHAUG_001056"
     CDS             347..787
                     /locus_tag="ACHAUG_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480162.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACHAUG_001057"
     CDS             799..1467
                     /locus_tag="ACHAUG_001057"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8480163.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACHAUG_001058"
     CDS             1531..2154
                     /locus_tag="ACHAUG_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8480164.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACHAUG_001059"
     CDS             2210..2596
                     /locus_tag="ACHAUG_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8480165.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACHAUG_001060"
     CDS             2809..4200
                     /locus_tag="ACHAUG_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8480166.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4811..5179
                     /locus_tag="ACHAUG_001061"
     CDS             4811..5179
                     /locus_tag="ACHAUG_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8480167.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5359..6300
                     /locus_tag="ACHAUG_001062"
     CDS             5359..6300
                     /locus_tag="ACHAUG_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8480168.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6369..7100
                     /locus_tag="ACHAUG_001063"
     CDS             6369..7100
                     /locus_tag="ACHAUG_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8480169.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7244..9424
                     /locus_tag="ACHAUG_001064"
     CDS             7244..9424
                     /locus_tag="ACHAUG_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8480170.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9444..9926
                     /locus_tag="ACHAUG_001065"
     CDS             9444..9926
                     /locus_tag="ACHAUG_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8480171.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9942..10703
                     /locus_tag="ACHAUG_001066"
     CDS             9942..10703
                     /locus_tag="ACHAUG_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8480172.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10834..12168
                     /gene="murC"
                     /locus_tag="ACHAUG_001067"
     CDS             10834..12168
                     /gene="murC"
                     /locus_tag="ACHAUG_001067"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8480173.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12475..12960
                     /locus_tag="ACHAUG_001068"
     CDS             12475..12960
                     /locus_tag="ACHAUG_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8480174.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12953..13636
                     /locus_tag="ACHAUG_001069"
     CDS             12953..13636
                     /locus_tag="ACHAUG_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8480175.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13762..16407
                     /gene="polA"
                     /locus_tag="ACHAUG_001070"
     CDS             13762..16407
                     /gene="polA"
                     /locus_tag="ACHAUG_001070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8480176.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16452..17288
                     /gene="mutM"
                     /locus_tag="ACHAUG_001071"
     CDS             16452..17288
                     /gene="mutM"
                     /locus_tag="ACHAUG_001071"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8480177.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17252..17881
                     /gene="coaE"
                     /locus_tag="ACHAUG_001072"
     CDS             17252..17881
                     /gene="coaE"
                     /locus_tag="ACHAUG_001072"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8480178.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17933..18265
                     /locus_tag="ACHAUG_001073"
     CDS             17933..18265
                     /locus_tag="ACHAUG_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8480179.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18577..19068
                     /gene="nrdR"
                     /locus_tag="ACHAUG_001074"
     CDS             18577..19068
                     /gene="nrdR"
                     /locus_tag="ACHAUG_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8480180.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19091..20464
                     /locus_tag="ACHAUG_001075"
     CDS             19091..20464
                     /locus_tag="ACHAUG_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8480181.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20464..21390
                     /gene="dnaI"
                     /locus_tag="ACHAUG_001076"
     CDS             20464..21390
                     /gene="dnaI"
                     /locus_tag="ACHAUG_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8480182.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21519..21959
                     /locus_tag="ACHAUG_001077"
     CDS             21519..21959
                     /locus_tag="ACHAUG_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8480183.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22103..22804
                     /locus_tag="ACHAUG_001078"
     CDS             22103..22804
                     /locus_tag="ACHAUG_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8480184.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            22801..23922
                     /locus_tag="ACHAUG_001079"
     CDS             22801..23922
                     /locus_tag="ACHAUG_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8480185.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            23937..25169
                     /gene="pepT"
                     /locus_tag="ACHAUG_001080"
     CDS             23937..25169
                     /gene="pepT"
                     /locus_tag="ACHAUG_001080"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8480186.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25301..26209
                     /locus_tag="ACHAUG_001081"
     CDS             25301..26209
                     /locus_tag="ACHAUG_001081"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8480187.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26244..26516
                     /locus_tag="ACHAUG_001082"
     CDS             26244..26516
                     /locus_tag="ACHAUG_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8480188.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26527..27573
                     /locus_tag="ACHAUG_001083"
     CDS             26527..27573
                     /locus_tag="ACHAUG_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8480189.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            27824..30433
                     /gene="clpB"
                     /locus_tag="ACHAUG_001084"
     CDS             27824..30433
                     /gene="clpB"
                     /locus_tag="ACHAUG_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8480190.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30584..31270)
                     /locus_tag="ACHAUG_001085"
     CDS             complement(30584..31270)
                     /locus_tag="ACHAUG_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480191.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31248..31742)
                     /locus_tag="ACHAUG_001086"
     CDS             complement(31248..31742)
                     /locus_tag="ACHAUG_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8480192.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(31762..32982)
                     /locus_tag="ACHAUG_001087"
     CDS             complement(31762..32982)
                     /locus_tag="ACHAUG_001087"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8480193.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33129..34964
                     /gene="lepA"
                     /locus_tag="ACHAUG_001088"
     CDS             33129..34964
                     /gene="lepA"
                     /locus_tag="ACHAUG_001088"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8480194.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35160..>36042
                     /locus_tag="ACHAUG_001089"
     CDS             35160..>36042
                     /locus_tag="ACHAUG_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480195.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNL"
BASE COUNT        12145 a         6294 c         7719 g         9884 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     4801 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     4861 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     4921 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     4981 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5041 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5101 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5161 tgcggttaca ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc
     5221 gaagtctttt taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta
     5281 tacacaagca aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa
     5341 gaaggaagga agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc
     5401 gaagaaccag aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa
     5461 ggtcgtattc tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac
     5521 acgactgcct atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag
     5581 atcgatccaa gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt
     5641 gtaagtttaa agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca
     5701 ccgcaagaat ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat
     5761 gattatgaat atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt
     5821 cgagagcttc cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg
     5881 acctactgta ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga
     5941 ttcaaagatg tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc
     6001 caaggagaat tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc
     6061 aaccaagtag ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga
     6121 tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa
     6181 gaaaaacatt taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt
     6241 caagaccatg gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag
     6301 aaaacaatag ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg
     6361 tgatccacat gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt
     6421 ttgaaaaact tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc
     6481 tcaatgatcg aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag
     6541 aaacaggagc catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat
     6601 caccggagga ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc
     6661 tgattcaccc aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat
     6721 cgagatattc agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa
     6781 attatctttt tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc
     6841 taagagactt tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga
     6901 caattgtatt tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc
     6961 tctggattca ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc
     7021 atggggttgt ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag
     7081 gaccttggca tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct
     7141 ctttttattt ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat
     7201 gatagaataa agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa
     7261 ccagacgatc attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac
     7321 tactgtactt aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa
     7381 agaaatgact ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta
     7441 tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa
     7501 actgactccc gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga
     7561 tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg
     7621 acttgaacca ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc
     7681 agaaaaatat attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc
     7741 tcatgagatt attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc
     7801 ttatacgtat aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa
     7861 aggcgtatat gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg
     7921 aatattagct actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga
     7981 tgaagcagca attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc
     8041 ttcttcattc gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat
     8101 cgagcgagaa ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt
     8161 cggcgaaaac ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg
     8221 atttgatccg gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt
     8281 tttagcaatc gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc
     8341 tggaccagct tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa
     8401 tggtacagcc agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca
     8461 taaaacctat tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat
     8521 tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg
     8581 tcgcctgcaa gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg
     8641 ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga
     8701 aacagcggtt aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca
     8761 tatatcagga ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg
     8821 agaattcacc gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga
     8881 acaagcaatc ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat
     8941 ccatcccgaa agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa
     9001 agagcttggt acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc
     9061 tgaaagccta tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc
     9121 tggacgagat atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat
     9181 ggaagacttg aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg
     9241 cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa
     9301 aaaattcgtc aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat
     9361 agaacaagtc gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga
     9421 gtaacaatga ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga
     9481 tatcattaaa aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac
     9541 gtacaactgg cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat
     9601 ttgaagagtc agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc
     9661 atcttcatct agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga
     9721 aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt
     9781 attatcgatg ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca
     9841 aatatcgttg tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact
     9901 ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga
     9961 ggaatctaaa agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt
    10021 ttgaggaact gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg
    10081 ataaagcagc acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg
    10141 tggggcatgc gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag
    10201 atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg
    10261 ataaggcttt tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac
    10321 tggagattct tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga
    10381 cactgcaacg caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt
    10441 tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta
    10501 cagcaaaaga actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag
    10561 aaatggaacg tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc
    10621 ctcacattga tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa
    10681 aaacggattt ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt
    10741 tggttgaaat tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa
    10801 agaatggacg ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat
    10861 tttgttggta tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt
    10921 ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa
    10981 gcaaatatca cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt
    11041 gcaggaaatg catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg
    11101 gaagtgattc gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct
    11161 gtaacaggct ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt
    11221 gtccgtccaa caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa
    11281 ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat
    11341 gcaatcatga ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta
    11401 tttacagctt tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac
    11461 gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa
    11521 aacgatgata tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc
    11581 tatcatgggg atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc
    11641 ttaaatgctt taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt
    11701 gcagaagaaa tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca
    11761 gacatgaccg tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat
    11821 ggcgcacgcc aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt
    11881 acgcgtacga ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt
    11941 tacttatgcg atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac
    12001 cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg
    12061 ttagattatc atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa
    12121 caagcgtatg aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc
    12181 agtgtcctta taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt
    12241 tgcaacttat gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat
    12301 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
    12361 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
    12421 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
    12481 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
    12541 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
    12601 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
    12661 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
    12721 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
    12781 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
    12841 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
    12901 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
    12961 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
    13021 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
    13081 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
    13141 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
    13201 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
    13261 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
    13321 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
    13381 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
    13441 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
    13501 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
    13561 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
    13621 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
    13681 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
    13741 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
    13801 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
    13861 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
    13921 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
    13981 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
    14041 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
    14101 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
    14161 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
    14221 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
    14281 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
    14341 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
    14401 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
    14461 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
    14521 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
    14581 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
    14641 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
    14701 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
    14761 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
    14821 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
    14881 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
    14941 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
    15001 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
    15061 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
    15121 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
    15181 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
    15241 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
    15301 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
    15361 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
    15421 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
    15481 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
    15541 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
    15601 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
    15661 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
    15721 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
    15781 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
    15841 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
    15901 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
    15961 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
    16021 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
    16081 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
    16141 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
    16201 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
    16261 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
    16321 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
    16381 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
    16441 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
    16501 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
    16561 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
    16621 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
    16681 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
    16741 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
    16801 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
    16861 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
    16921 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
    16981 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
    17041 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
    17101 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
    17161 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
    17221 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
    17281 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
    17341 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
    17401 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
    17461 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
    17521 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
    17581 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
    17641 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
    17701 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
    17761 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
    17821 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
    17881 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
    17941 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
    18001 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
    18061 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
    18121 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
    18181 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
    18241 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
    18301 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
    18361 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
    18421 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
    18481 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
    18541 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
    18601 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
    18661 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
    18721 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
    18781 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
    18841 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
    18901 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
    18961 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
    19021 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
    19081 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
    19141 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
    19201 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
    19261 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
    19321 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
    19381 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
    19441 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
    19501 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
    19561 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
    19621 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
    19681 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
    19741 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
    19801 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
    19861 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
    19921 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
    19981 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
    20041 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
    20101 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
    20161 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
    20221 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
    20281 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
    20341 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
    20401 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
    20461 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
    20521 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
    20581 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
    20641 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
    20701 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
    20761 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
    20821 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
    20881 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
    20941 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
    21001 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
    21061 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
    21121 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
    21181 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
    21241 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
    21301 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
    21361 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
    21421 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
    21481 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
    21541 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
    21601 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
    21661 gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa
    21721 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
    21781 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
    21841 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
    21901 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
    21961 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
    22021 taaggagata tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata
    22081 attatctaga aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt
    22141 gggtgacttt gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt
    22201 acccgttgca ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca
    22261 aggtccttat gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac
    22321 cgtacgcctt gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac
    22381 gattgcgggt atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca
    22441 acgcttaagc ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg
    22501 atcttggctg caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa
    22561 aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat
    22621 ggagctgatg ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg
    22681 gacaagagag cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca
    22741 agaggcacgt caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc
    22801 atgacaatca gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta
    22861 gctgaagaag gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga
    22921 gtaatgatga cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc
    22981 gatttgctga ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat
    23041 cagccacaag aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca
    23101 cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt
    23161 attgaagtgg agagttattt agtaaagact catgagattc attataaaaa actggccgtt
    23221 tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt
    23281 acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg
    23341 gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca
    23401 aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca
    23461 gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg
    23521 tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa
    23581 caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa
    23641 tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca
    23701 atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat
    23761 atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag
    23821 caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt
    23881 ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt
    23941 acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa
    24001 caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag
    24061 aattagaaga gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg
    24121 gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg
    24181 atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg
    24241 agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa
    24301 atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag
    24361 cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc
    24421 cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg
    24481 gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg
    24541 gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc
    24601 aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag
    24661 caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg
    24721 aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata
    24781 tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc
    24841 aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat
    24901 attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag
    24961 caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt
    25021 caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc
    25081 acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa
    25141 aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt
    25201 tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt
    25261 gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt
    25321 ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta
    25381 tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc catcgattga
    25441 tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg
    25501 tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca
    25561 tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct
    25621 caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg
    25681 tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc
    25741 agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt
    25801 ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca
    25861 ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac
    25921 aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc
    25981 cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac
    26041 gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc
    26101 tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt
    26161 atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt
    26221 aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc
    26281 ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa
    26341 ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt
    26401 tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta
    26461 taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag
    26521 caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt
    26581 gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa
    26641 ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa
    26701 atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg
    26761 aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca
    26821 ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga
    26881 gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta
    26941 gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt
    27001 atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc
    27061 agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt
    27121 ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa
    27181 accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt
    27241 gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt
    27301 ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct
    27361 gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca
    27421 gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc
    27481 tatggatttg tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa
    27541 gtaaaaaaag agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat
    27601 caaatcaaag aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat
    27661 atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa
    27721 actatgtgta tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca
    27781 aagaaataag tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat
    27841 gactacgacg cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca
    27901 tcaagatatc gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg
    27961 aaatttctat acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct
    28021 actggatgag tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca
    28081 gaatttcttt cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt
    28141 tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt
    28201 ttatttgaat aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag
    28261 aggaggagaa agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata
    28321 cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga
    28381 tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct
    28441 gataggagaa cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt
    28501 acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc
    28561 attgatcgct ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa
    28621 agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt
    28681 aggggcagga aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc
    28741 tcgcggtgaa ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga
    28801 aaaagacaaa gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga
    28861 agatacgatc agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa
    28921 catccatgac aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg
    28981 gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga
    29041 aatgaactct atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat
    29101 cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt
    29161 gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac
    29221 agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca
    29281 cgaattagaa gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg
    29341 tacgatacct caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga
    29401 gattaagatg gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt
    29461 aacgggtatc cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa
    29521 tgaaacactt cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc
    29581 ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt
    29641 cctaggacca actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt
    29701 tgactcagaa gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt
    29761 gtctcgtcta gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac
    29821 ggaagctgtc agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca
    29881 tcccgatgtc ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa
    29941 agggcgagta gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca
    30001 gttactgctg gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt
    30061 caacacgcta ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat
    30121 tttatttaca ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca
    30181 attggcacaa cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac
    30241 gtggattgct gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat
    30301 cactaaggaa gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa
    30361 atcaaaagta acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga
    30421 agaaatcgtc taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt
    30481 gtcagacctc cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc
    30541 cgaaatttct tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt
    30601 tttctaattt ataactttca aagtaacgct catctttttt actttgagac catgcaaaca
    30661 atcccattaa ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg
    30721 ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta
    30781 agaactggtc gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg
    30841 caaagccgaa taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca
    30901 agtaaccaat caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga
    30961 ttttgtccca aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga
    31021 tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt
    31081 tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg
    31141 tgattccttg agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa
    31201 cgaatacacc aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa
    31261 gtccctccat ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact
    31321 tttccgttca atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca
    31381 gctgtttctt gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca
    31441 gtatataagc cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc
    31501 ccggcatcga tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt
    31561 tctgtgtcag caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct
    31621 tcattttctt gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact
    31681 aactgttggt tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc
    31741 atctaaacat catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat
    31801 gatacgctct agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa
    31861 ataagaagca cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac
    31921 ggattttcct ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc
    31981 gaagacacca ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc
    32041 tgttccaaca aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa
    32101 atatggttcg atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt
    32161 tttataaata tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca
    32221 taagccattt cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc
    32281 agctacccag tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc
    32341 tgtgcgctca tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa
    32401 taaagcacca tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc
    32461 aactggtaat cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct
    32521 gattttaaag gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat
    32581 actgtaagga tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac
    32641 gataattaga tcttgcatca atccatggtt agcatattct ggataagttc caactccagt
    32701 cacaccaccc attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat
    32761 ttgagagatc agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg
    32821 ttttttggaa cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc
    32881 agtcacttgg aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac
    32941 cggttttaat tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat
    33001 tagcttgata acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa
    33061 aactctttaa attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag
    33121 gagacgctat ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga
    33181 tcatcgctca tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc
    33241 acactgtgac gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg
    33301 aacgtggaat cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag
    33361 aaacatatat ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct
    33421 ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg
    33481 aagcacaaac attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag
    33541 tcatcaataa aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag
    33601 acgtaatcgg aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga
    33661 ttgaagatat tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg
    33721 caccgctaaa agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa
    33781 atgtccggat cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg
    33841 gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact
    33901 ttttgatggt cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac
    33961 gagtagggga tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc
    34021 gaaaaatgaa tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg
    34081 atttgcggga agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag
    34141 aaacctctca agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg
    34201 atgtcgtcca agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat
    34261 ccgttattta ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag
    34321 attttcctga acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca
    34381 tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat
    34441 tcattacgat ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt
    34501 ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat
    34561 tggattatga aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga
    34621 atggagaaaa agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg
    34681 gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa
    34741 tccaagcagc tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa
    34801 acgtcttggc taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac
    34861 aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct
    34921 tcatggccgt tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa
    34981 aaacctatta aataaacctt tttgagtaga tgcaacactg aggattatct gtataaaaga
    35041 ttttaaactg tcatttggtt atcacggcga tactaaaatt aacaaaattg gaatattttt
    35101 cttgaagatt tttagctgtc agtatatgat gaaagcatct cataggaggt gcttttttca
    35161 tggttgataa ttcaagtgga agggttctta aatctctaag aaaagagaaa aaactatctc
    35221 aaaaaaagtt agctgattta gcaggtattt cacaaagtac tttagttaaa tatgaaaaag
    35281 ggtcaagaaa aatccctaaa gacgtagata atactctaag caagattctg aatatagaaa
    35341 ctctaataaa agatgaggaa gataagattg agattctcat cggtaaacta attgcataca
    35401 gagatatgaa taagttactt aacaaagagt tagctgataa tataggaatt agtgaagtat
    35461 tactcagtta tgtgttaaat agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg
    35521 atatttttct attaagtaat gaaaaagaaa ttttgaagga gattaatcgt gatagtgaga
    35581 tatttagttt atcaaaagac gataaaattg ttatgggaaa aagaatacga gaagtccgaa
    35641 agaaccggga agagacgctt gaaaaatttg gtaaaaactt taccatctat acaggcaaaa
    35701 atgttataag tagatgggaa aaaggtatta atattccaga tattgaaaaa ctaatgaata
    35761 ttgcctatct tggtaaggtc actgttcctt accttatgta cggagaagat tataagaata
    35821 tacttccaaa agatgaaaga gttagcgatt ttaaaaagat aaattctttt agtatgggat
    35881 taaggatgag gaaaattagg aaagattatt atctagaaag ggaggagttt ggaaagcttt
    35941 tttctccttc aatttcaaaa tggtccatag ataggtatga gaatggtaga gatataccca
    36001 atactaatcg aattatccaa tatgcttata taggaaatct tt
//
LOCUS       DAYYBH010000018        35017 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000018 DAYYBH010000000
VERSION     DAYYBH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.018"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1917
                     /gene="efbA"
                     /locus_tag="ACHAUG_001090"
     CDS             211..1917
                     /gene="efbA"
                     /locus_tag="ACHAUG_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8480196.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2075..2977)
                     /locus_tag="ACHAUG_001091"
     CDS             complement(2075..2977)
                     /locus_tag="ACHAUG_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8480197.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2974..3666)
                     /locus_tag="ACHAUG_001092"
     CDS             complement(2974..3666)
                     /locus_tag="ACHAUG_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8480198.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3709..4350)
                     /locus_tag="ACHAUG_001093"
     CDS             complement(3709..4350)
                     /locus_tag="ACHAUG_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8480199.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4388..4702)
                     /locus_tag="ACHAUG_001094"
     CDS             complement(4388..4702)
                     /locus_tag="ACHAUG_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480200.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4690..5319)
                     /gene="pyrE"
                     /locus_tag="ACHAUG_001095"
     CDS             complement(4690..5319)
                     /gene="pyrE"
                     /locus_tag="ACHAUG_001095"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8480201.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5326..6042)
                     /gene="pyrF"
                     /locus_tag="ACHAUG_001096"
     CDS             complement(5326..6042)
                     /gene="pyrF"
                     /locus_tag="ACHAUG_001096"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8480202.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(6039..6971)
                     /locus_tag="ACHAUG_001097"
     CDS             complement(6039..6971)
                     /locus_tag="ACHAUG_001097"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8480203.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6968..7744)
                     /locus_tag="ACHAUG_001098"
     CDS             complement(6968..7744)
                     /locus_tag="ACHAUG_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8480204.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7798..10983)
                     /gene="carB"
                     /locus_tag="ACHAUG_001099"
     CDS             complement(7798..10983)
                     /gene="carB"
                     /locus_tag="ACHAUG_001099"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8480205.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10988..12064)
                     /locus_tag="ACHAUG_001100"
     CDS             complement(10988..12064)
                     /locus_tag="ACHAUG_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8480206.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12069..13349)
                     /locus_tag="ACHAUG_001101"
     CDS             complement(12069..13349)
                     /locus_tag="ACHAUG_001101"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8480207.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13368..14294)
                     /locus_tag="ACHAUG_001102"
     CDS             complement(13368..14294)
                     /locus_tag="ACHAUG_001102"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8480208.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14307..15590)
                     /locus_tag="ACHAUG_001103"
     CDS             complement(14307..15590)
                     /locus_tag="ACHAUG_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8480209.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15604..16143)
                     /gene="pyrR"
                     /locus_tag="ACHAUG_001104"
     CDS             complement(15604..16143)
                     /gene="pyrR"
                     /locus_tag="ACHAUG_001104"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8480210.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16378..17289)
                     /locus_tag="ACHAUG_001105"
     CDS             complement(16378..17289)
                     /locus_tag="ACHAUG_001105"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8480211.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17286..17750)
                     /gene="lspA"
                     /locus_tag="ACHAUG_001106"
     CDS             complement(17286..17750)
                     /gene="lspA"
                     /locus_tag="ACHAUG_001106"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8480212.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17755..18453)
                     /locus_tag="ACHAUG_001107"
     CDS             complement(17755..18453)
                     /locus_tag="ACHAUG_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8480213.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18440..18826)
                     /locus_tag="ACHAUG_001108"
     CDS             complement(18440..18826)
                     /locus_tag="ACHAUG_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480214.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18973..20643)
                     /locus_tag="ACHAUG_001109"
     CDS             complement(18973..20643)
                     /locus_tag="ACHAUG_001109"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8480215.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20826..21059
                     /locus_tag="ACHAUG_001110"
     CDS             20826..21059
                     /locus_tag="ACHAUG_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8480216.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21158..21589)
                     /locus_tag="ACHAUG_001111"
     CDS             complement(21158..21589)
                     /locus_tag="ACHAUG_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8480217.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21668..22081
                     /locus_tag="ACHAUG_001112"
     CDS             21668..22081
                     /locus_tag="ACHAUG_001112"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8480218.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22119..23267)
                     /locus_tag="ACHAUG_001113"
     CDS             complement(22119..23267)
                     /locus_tag="ACHAUG_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8480219.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23389..23985)
                     /locus_tag="ACHAUG_001114"
     CDS             complement(23389..23985)
                     /locus_tag="ACHAUG_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8480220.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24185..26827)
                     /gene="alaS"
                     /locus_tag="ACHAUG_001115"
     CDS             complement(24185..26827)
                     /gene="alaS"
                     /locus_tag="ACHAUG_001115"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8480221.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27215..27646
                     /locus_tag="ACHAUG_001116"
     CDS             27215..27646
                     /locus_tag="ACHAUG_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8480222.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27691..29040)
                     /locus_tag="ACHAUG_001117"
     CDS             complement(27691..29040)
                     /locus_tag="ACHAUG_001117"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8480223.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29110..30273)
                     /locus_tag="ACHAUG_001118"
     CDS             complement(29110..30273)
                     /locus_tag="ACHAUG_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8480224.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30402..30725
                     /locus_tag="ACHAUG_001119"
     CDS             30402..30725
                     /locus_tag="ACHAUG_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480225.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30908..32257)
                     /locus_tag="ACHAUG_001120"
     CDS             complement(30908..32257)
                     /locus_tag="ACHAUG_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8480226.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32326..33279)
                     /locus_tag="ACHAUG_001121"
     CDS             complement(32326..33279)
                     /locus_tag="ACHAUG_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8480227.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33381..34571)
                     /locus_tag="ACHAUG_001122"
     CDS             complement(33381..34571)
                     /locus_tag="ACHAUG_001122"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8480228.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34771..>35017)
                     /locus_tag="ACHAUG_001123"
     CDS             complement(34771..>35017)
                     /locus_tag="ACHAUG_001123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480229.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9968 a         7650 c         6130 g        11269 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgataccta
       61 aaaatttgga tttcaacgat agaaacactg ttttttatgt aattagtcca agatttttct
      121 agacttttgc tttactattc ggccaaattg gtttttcttc tttttttcgt tataatgaat
      181 atacttatga aaagaaatga ggaatgaacc atgtcctttg atggtgtatt tacccacgcg
      241 atggtcaacg aattgcgtga aacgttacta tctggccgga tctcgaaaat ccatcagcct
      301 tatgaaaatg aaatcgtgct ggtgattcgt tcacgcggca aaaaccatcg attattatta
      361 tctgcgcacc caagttatgc tcgtgtacag attacccaaa tcgattatca gaatcctgat
      421 aatccgccta acttcgtgat gatgcttcga aaatatttgg atggcgctat tttagaagac
      481 atcgaacaaa tcgaaaatga tcgagtgatc catttccact ttgctaaacg aaacgagttg
      541 ggtgatctac aaaatatcat attgatcgtt gaattaatgg gacgtcatag tacgatcgtc
      601 ttagttaatc gagaaactgg aaaaatactg gatgcgatca aacacattgg aagctctcaa
      661 aacacttatc gttctttgct tcctggtgta gaatatgttg ctccgccaaa acaagaggtc
      721 ttgaacccat ttagctccga gaaagaaaaa atctttcagc ggctttccca aactgaacta
      781 gatccaaaag caatccaacg tcagtttcag ggaatcggtt tcgatatcgc tcaagagctg
      841 accaaaagat tactggaacg tcctaatgaa aagatggttg tatgggacga atttttctca
      901 gccatcagcc atcagcctat tcctactttt tacgaaacgg aaaataaaga ttttttcaca
      961 ccgattgctt atcaggtatt gtccgaacag gcatctgctg taacagctta tccaacactc
     1021 agtcaattat tagatagcta ttatcacgaa aaagcagaga aagatcgggc aaaacaacaa
     1081 ggcggagaac tgattcgaaa aatcgaaaat gaactaaaac gaaataaaaa taaattgaaa
     1141 aaacgtgaac aaacactcaa agaatccgaa aacgctgaga attaccgtag aaatggcgaa
     1201 ctacttacta cttttctgac acaagtacca cgcggcgcaa aagaagttgt tttacctaat
     1261 tattatgaag aagatcggcc aatcaaaata gcactagatc cggcattgac accaaatcaa
     1321 aatgcacaga aatatttcca tcgataccaa aagctgaaga acgctgttaa gctgattggc
     1381 gaacaaatcc aagaagccaa agatgaaatc caatatcttg aatctgtctt atctcaactg
     1441 gaaattgccg gcccaatgga tatcgaagca attaaagaag aattgacttc agaaggatac
     1501 ctgaaaaaga agaacttaaa aaaacaaaaa cgtaagaaac catctcagcc tgatcaatac
     1561 ttttcttctg acggaacgct cattcttgtt ggtaaaaaca atcttcaaaa tgaccagctg
     1621 acaatgaaga cagctaaaaa aaccgactat tggcttcatg caaaaaatat acccggttcg
     1681 cacgtcatca ttaaaagtga caagccctca gatgaaacaa ttacagaagc tgccgagtta
     1741 gctgcctatt tttcaaaata ccgatactct gcccaagtgc cggttgatct tgtacaggta
     1801 aagcatatcc gaaaaccaaa tggcgccaag cctggttatg ttatttatga aaaccaaaag
     1861 acgattatcg tgacaccaga agaagaaaaa attacagcta tgaaacaaaa caggtaatca
     1921 atgagtgttt ctagaatcat atttgcgtac tttcccacaa cagcaattag ttataaattg
     1981 tttgctacaa aaagaggagt tgtgacaaaa ttctgtcaca acccctcttt tttgatcaac
     2041 ggtgttcaat aaacagcgtt ctcatctgct tttcttagct ctgctggtcc agtaaccgag
     2101 taagctcttt gataaatctt tgagcgattt cactgagcgg acgtttcgcc gaataaatga
     2161 tataaaattc atttagttcg ccttctgcca aagggattaa ccgcaagtgc tcgcgaatcc
     2221 ctccaatcac aataccagat cctgaagcgt aagcatcagt ttcaagaacg atattcatca
     2281 aagtacctcg atcactcgta taaatcactg tttcttgatc agggatctcg ataaggtctt
     2341 cataaaaata cgggaaatta tttccatcct gtgtaaaacg aacttgagga taagataaca
     2401 attcttcttt ggtgatctct gcgtgttcag ctaatggatg tccttgcctt aaaaaaatat
     2461 gcgttttgaa ttttccaatg ctgcggccac ttagctctcc ttgttgtaaa tatcttctga
     2521 taccagcttg attatgctca ttcatataaa taatgccgat ctcactgtgc tgctctttga
     2581 cttcttcgat cactctcgct gttgtcgtct ccatgatccg aaattgtttg atctcgtctt
     2641 tgaattcctc gagtagctgt gcggtcaaag gacctaagaa atcataatgc tgggcagcga
     2701 tcgaaaaatt gtctctcttt tcactcgtta gatagtgagt ttctaatgta tcaagctgga
     2761 ccaatatttt ctcggcacga tttaagaagt cttgcccttc ttctgttaaa tatgcccctc
     2821 gattcgttcg agtaaacagc gtgatcccta actcggtttc taattctttg ataccagctg
     2881 aaagacttgg ctgggagaca aacattttct ttgccgcttc tctgaaacta ccattgtttg
     2941 ctacagcgac cacgtatttc atctgctgaa tattcattgc tcaaactcct cgaaataata
     3001 aatttctctt ccattcaagg gctggattgg tcggttgttc ggtcgagcat actgaccatc
     3061 gaaggggaga agaaactctt ggttcagttt tcctacatga tccattacaa accattggat
     3121 tggcccgctg gtaggcggag ttgtcaatga gccaatataa tggaaatggc ttttttgctc
     3181 cggaaagaac acatccggat ttacccaatg ctcatgtttt tctaagttcc aactttcccc
     3241 attttcttga ttatacagcc aaccttcctg taccattagc tggtacatga cacccacaac
     3301 tagattctct tttttgctgt tcatatgaac aaaatgaaat tcaatatctt cctgttttcc
     3361 gttgatgata tgttcagaag gcaagtggaa atgaatatct gtcaaataat agcgttcttt
     3421 cttgaactct acataactga gttggtcaaa aggtactaaa tgaatcgtgt ttttgaattc
     3481 cttgtcagta aaccgttgct tttgataatg aaaggaaagc gtgtctcctt ctattttggt
     3541 ttcttccccc ttggtcaaat gaataggcga ctgcaatgga aattccgcac ctctcgcaaa
     3601 ccaatcacat aaactcgccc aattttccgg acctttgtct ccagaatagc cccattcgac
     3661 atccatattc tttttcaact atacttcccc ctgcatctca tatcattttt atttttcttc
     3721 tttctttaca atcgttgaga cggtttctaa aaacgcctct tttccataaa attcctggtt
     3781 caatagttcc ggaaatatct ctttaaagaa atactgaaca gaaggccgat gttcaaagct
     3841 tttaatcgta gtcactgcct caatgaacat ctccttataa actttttccg ggttattttt
     3901 gatgatttca tcaaaagatt cataaagaag gtcaacctta tctgaaatgg ctaatagttc
     3961 tccttctacg gtttcatctt ttccttcgaa caaccgacgg cgataaactt cttggaactc
     4021 agctggaatc tcttggttga tgaattcgtc cgtcatcttt tcttcaactg tttgcagcat
     4081 ccctcttaac tctttgcttg cgtatttcac cggcgttttg atatcaccaa taaaacgctc
     4141 tgtgtagtca tgattcaatg atttttcata gagcagtttc caatcgatcg gttgtccatt
     4201 cgcttcttca atgtctccta gaacttgcgc aatagatgct acacgatacg aatgtgctgc
     4261 aactgtatgc tctgtatatt taaaaaatcc aggtgccctt gtgatcgtct ctaaattgtt
     4321 caatcctaaa ataaattcat tcaaccccat aaaagttacc tccctttgag atttcttttt
     4381 ccgaatatca tatcattaaa taactgatat tcgcaacatt tccgctcttt accatttttg
     4441 aaacagctcc cttaccaaaa aatagagaaa atctatatat aatagaaaaa agagaagaga
     4501 ttattctctt ctttctgcta accagtctta gaaaaatgaa ttttaaaaaa atcaaaaact
     4561 gatcacgata gaagtttctt ttcatacctg atgaaaagat gcttctcgca atcagttttt
     4621 ggatttttgc tattccaatt gatgattctg gagacacaaa tcggatttct ctatcttcat
     4681 caagcgcgct taaatctgcc aattttcagg atcttttttc cattctttta gcagagttaa
     4741 ttcttgctca tcgatataac gatcttctaa agctgcttca atcaatacag aataattggt
     4801 caacgtcata agagggattt cggctttttc gaaatttgct tttccttttg gcaactcata
     4861 cgtaaagata gccgcaacac caaggatatc cgcaccttct ctttttgctg cttcagctgc
     4921 ttccaagaca cttccgcctg tagaaatcaa atcctcgatc acaaccatct tctgaccttc
     4981 aaatatacgt ccttcgattt ggttaccttt cccatgatct ttcgccttac ttcgaatata
     5041 gaccattggc aaatccagta tttccgctac ccatgcggca tgagggattc ctgctgttgc
     5101 agttccagca atcacttgta cttcagggta agcttctttg attttgcttg ctaatccttt
     5161 tgcgatctct tttcggactt ttggatagct catcgtgatt cggttatcac agtaaattgg
     5221 gcttttgatt ccacttgccc atgtaaaagg attacttgga tttaaaaata ctgcttcaat
     5281 ttctaataag tcttttgcga tactttgttc aacattcatt ttaggttagc tccattctgt
     5341 ttgaatcgtt tgatatgctt tataaggatt agttgactga gtgattggtc gacctataac
     5401 gataaaagtt gagccgatct tttttgcctg agaaggtgtg actactcttg tctgatctcc
     5461 tgcgactgtt ccttctggtc ttatacccgg agttaagcaa acaaattcgt ctcctgccat
     5521 ttctttgatc gcttctactt cccaagcaga agaaaccaca ccatctaatc ctgctttttt
     5581 ggcacagcta gcataatgga tcacactttt ttctaaagaa acttcgatta gttgatcggc
     5641 atgcatttct tcttcacttg tagaagtaag ctgagtgata gctagaagta gcggacgttt
     5701 tccaccttct ggagtcccct cttctaatcc tcgcattgcc gcctccatca tacgaatacc
     5761 gccagcggca tgaacacagg tgagatcaac accaagcttt gccaatcccc gcattgcttt
     5821 ttcgaccgta ttcggaatat cgtgaagttt cagatctaaa aatacattat gaccagcctc
     5881 ttttagataa cgtacgattt caggtccttc ttggtagaac aattccattc cgattttcac
     5941 aaacaatttt tcttctttcg ggaaaaatcg aagaaagtct tctacctctt gcctagttga
     6001 aaaatctaat gcaataattg gtcgttggtc tagttgattc atcgttcacg ttcctctctc
     6061 acttctttga ttaatctttc cagcgattcg atgcctagtt cgctcattcg cacagggagc
     6121 tctttaatta gtttaggaca gatatacgga tcggtgaaat tcgcggtccc tatagctaca
     6181 gcacttgctc cagccataaa catctcaaga acatcatcca ctgtctgcac gccgcccatc
     6241 ccaatgattg gaaggctgga tatacttgct acttggtgaa tcaaacgaat agcgacaggc
     6301 ttgattgctg gtccggagag tccacctgtt tgattggcta aaatcggttt tcttgtctta
     6361 aggtcaatcc gcataccaag aagagtattg atcatcgtaa acccatctgc ccctccagct
     6421 tcgattgcct gagcgattgg aacgatgtct gttacatttg gggacaattt gacatagaca
     6481 ggaacagaag aaactttctt tactgcttgt gttaattgaa aagcaacatc agaatctgtt
     6541 ccaaaagcaa ttccgccatg tttgacattt gggcaagaaa tgttcagctc gattgctttc
     6601 acatttggtg cttcgccaat tttgctacaa acttctacat agtcttcttc acaagcacca
     6661 gcaacatttg cgatgatggg taactcattg tactcctcta attctggaag aagtttatgc
     6721 ataacaacgt ctagaccagg attttgcaaa ccaattgcat tcaacatgcc agatggtgtt
     6781 tccgccactc ttggcgtggg attgccaaag cgggcatttg gagtcgttgc cttgaccatg
     6841 atcgatccaa gctgattcaa atcatagtat tttccgtatt ctttgccaaa accgaaacag
     6901 ccgcttgccg gcatgatagg attttttaat tctaggccag gaagtttgac cgctaaagga
     6961 ttctcattca tataattacc tctcctattt taaaaatcgg tccttcgtca caaactttca
     7021 cactttttgt tcctgtttca tcttccggcg tatgacaaac gcaagcatag caagccccta
     7081 tcccacatgc catgcgggat tctaatgata actggacatt ttgatgcgaa gaaaacagct
     7141 gttccactgt cttcaataac ccgtttgaac cacatgcaaa aacagctgta ggttccgtct
     7201 tcgttcctaa caataaatta ccgacatttc cgtgcatacc gtatgttcca tcgtcagtag
     7261 cgattcttgt ttctcctaga cgattaaatt ctttttcata ataaacgact tcatgtgtgg
     7321 caaaacctaa aaaatgcgtt ggcttaattc ctttttcgac taactgtttt gataactcat
     7381 atagaggagg gacaccgatt cctccaccga ttataaatac ttcgtctcct gtatgaagat
     7441 tggacaaatc aaaaccattg cctaaaggac ccatgacatc taaatggtct ccggcagaaa
     7501 gagtagagaa gactctagtc ccctcacctt ccacccgata aatgatacgg caagtatttg
     7561 tttcgtggtc gatatgattc aagctaatcg gtcggcgtaa tagcaagtct gcacgcggca
     7621 cgagaatatg caagaactgt cctggttgct gcatttcttt tactagatca cctttcaatg
     7681 tcatttcaaa aatctttggt gccagctgtt tttgctgaac gatttccatc atttcttgtt
     7741 tcatctgtcc cctcctgcaa cggtttttcg tctttttgtc ttaacatgtg ctcgccgtta
     7801 aatagggtta gttgaaaaag cacgggattc catcactcga acgatcgcat ctgctgtgtc
     7861 taaagaagta aacaatggaa taccatgttc aactgcttct cttcggatga tgaaaccgtc
     7921 tttagaagca tttccccgat ctttatcaat cgtattgaca accacttgtg ttttaccatc
     7981 tcttattaag tcgactacat ttttttctgc tgcttcctca gaaattttcg ctactgtttc
     8041 aacgaccaga ccattttttt cgaaataagc agctgtatgt ttcgttccta tcagactata
     8101 gccaatttct gtaaaacgtt tagctaattc tagtgcttct tcttttgttt cgtccgcaat
     8161 cgtaaataat acggctccat attcaggcaa gtgtaatcca gaagcttcga atgccttgta
     8221 caaagctttg tctaattgtc tgtcagatcc cattacttct ccagtcgatt tcatttcagg
     8281 ccctaagtaa gtatcaactt tctgcaattt cgtaaaagaa aagacaggtg ctttaacatg
     8341 aacttctttt gtttctggat aaagtccgtt ttcataacca agtgtcttta gtttctctcc
     8401 caaaatagct ttcgtcgcaa tttgtgccat tggaattcct gtcaccttac ttaagaatgg
     8461 aacagtccga cttgctcgag gattgacttc gatcacataa acctcatttt catggatgac
     8521 aaactggata ttcatcatcc cgacacaatt caaacctaat gctaagcgga tcgtgtaatc
     8581 ttcgatcgtt ttcttgatcg attcagataa tgtttgaggt ggatacacag ccattgaatc
     8641 ccctgaatgg acaccagcac gctcaatatg ctccatgatc cctgggatga gtactgtttc
     8701 accgtcacaa atcgcatcca cttcgcattc accaccaagc aagtaacggt ccactagtac
     8761 tggatgttct ggagatgctt tcacagcatg ttccatgtaa tcttctaaat ctcgctggtt
     8821 ttcaacgatt tccatcgcac gtccgcctaa tacatagctt ggacgaacaa gaactggata
     8881 accaattctc gaagcgataa ccacggcttc ttctttactt gtagcagtat cccctaaagg
     8941 ttgaggaata tccaactctt tcagcgcttg ctcgaataaa tcacgatttt ccgcacgatc
     9001 caagtcttcg atcgttgtcc ctaagatttt tacaccagca gcagtcaaag gttcggctaa
     9061 gttgattgct gtctgaccac caaattgaac gattactcct atcggctgtt ctagctcgat
     9121 cacattcatc acatcttcga aggtcaatgg ctcaaaatat aatttatccg aaatagaaaa
     9181 gtcagtcgaa actgtttcag gattgctgtt catgatgatt gcttcgtatc ctgcctgctg
     9241 gattgctttg acagaatgca cagtcgcata gtcaaattca accccttgtc cgatacggat
     9301 aggaccagat cccagtacta gaattgaggg cttttctgat cgtgaacttt cattttcata
     9361 ttcataggaa ctataaaaat aaggtgtttt tgattcgaat tccgctgcac aagtgtcaac
     9421 catcttatat acaggaatca aatcattttc ttttctcatc tggcgaacgg cttcttcgtt
     9481 tatttcccac agctccgcca cttttttgtc tgtaaaaccg ttttgtttcg ctgttttcaa
     9541 tatcttgtca tccaacggct gctgtttcaa ctgctgttcg atttctacga tatgcaacag
     9601 tttatctaaa aagaataaat cgatttttgt caaatccgct aattcttcta tggtgtaccc
     9661 tcttctaata gcttcagcta cataaaacag gcgatcatct tgtgctcgaa ccactttttg
     9721 gattaggtca tcgtccgtga tgtctgccaa ctcaggaagt tcgatatggt gtgtaccgat
     9781 ttctaaagaa cgcacagctt tcaataaaga ctcttcgata ttccgaccga tagccatcac
     9841 ttcaccagtt gctttcatct gcgtgcctaa aagccgttct ccgttttcaa acttatcaaa
     9901 cggccagcga ggaattttag ctacaacata atctaatgca ggttcaaatt ctgcaaaagt
     9961 cgttcctgta acaggatttt tcatttcgtc taatgttaat ccaagcgcta ttttagcagc
    10021 tagcttagcg atcggatagc cagtcgcctt acttgctaac gcagatgaac gagaaacacg
    10081 cggattgact tcaatcacat aataattaaa actatgtgga tcaagagcga gttggacatt
    10141 acagcctccc tcgatcttaa gtgctcgaat aatcgaaaga gaagcatcac gaagcatttg
    10201 atattcatgg tcagacaatg tttgactagg ggcaaatacg atagaatcac ctgtatggat
    10261 acccaccggg tcaaaatttt ccatattgca gacaacgatt gcattatcag ccgaatctct
    10321 catcacttca tattcaattt ctttaaaacc tgcaatactt cgttcaatca aacattgggt
    10381 agcgggggaa agacttaatc cattttctga tacgatacgt aattcttctt cattttcaca
    10441 catacctcca ccagttccgc ctaaagtaaa ggctggacga acgatgacag gatagccgat
    10501 tttatttgca aaatctacag cttcttctac tgtattgacg atttcagact cggggattgg
    10561 ctgattcaat tctttcatca actgtttgaa tagatcacga tcttctgctt gatcgatcgc
    10621 agaaagcttt gttcctagaa gttctatgtt caattcttct aaaatacctg atgctgccag
    10681 ctccattgcc atattgaggc ctgtctgtcc gcctaaagtt ggaaggagag catctggacg
    10741 ctcttttcga agaatccttg atacaaattc aagtgtgatc ggttcgatat atacttgatc
    10801 tgccacttct ttatccgtca tgatcgtcgc aggattagaa tttactaaaa ccacttcata
    10861 tccttcttct tttaaggcaa ggcaggcttg tgttcctgcg tagtcaaatt ctgcggcttg
    10921 accaatgatg atcggaccag atccaatcac cataattttc ttgatatctg tacgtttcgg
    10981 catctgttta ctgctccttt cctgcgtcca tcatttccat aaattcatca aataaatgta
    11041 gtgcatcatg cggaccaggg gcagcatctg gatgaaactg cacactgaaa gcgggataat
    11101 ctctgtgttt gactccttct actgttccgt catttacttc tacatgagtt acgatgagtt
    11161 tttctggatc aatcgtggct tcatcgactg catagccatg attttgagaa gtaaaatcaa
    11221 ttcttccggt cgctacttct cgtaccgggt gatttaatcc tcgatgtccg aatttcattt
    11281 tataggtatc tgcaccgttg gctaaagcaa ataactggtg accgagacaa ataccaaaaa
    11341 tcggtacttt tccttggatt tttttgatca tttcgattgc ttctggcaca tcttttggat
    11401 caccaggtcc attcgtcagc atgactccgt ctggtgatag cgctaaaatc gtctcggcat
    11461 ctgtgttata gggaagtact gtgaggttac agtttctttt actcaattct cgtaaaatac
    11521 tatgtttcaa gccgaagtca atgacaatga ctttccgacc tgttcctgga cttggataag
    11581 gtttgttagt tgagacttgc tgcacttgat tggttgctag aacggttgct ttcagctgat
    11641 caaatgcatg ttcgaaggaa tctccagcat caactatact agctttcatc gttccgactt
    11701 cacggatttt gcgagtaagg gcgcgcgtat cgatacctga tattccagga attccttttt
    11761 gtttcaaaaa tgaatctagc gtcatttggt ttcgccagtt tgaagctaca cgtgcatgtt
    11821 cttttactac tactcctttg catgttggag aaatcgattc ataatcatct ctatttaccc
    11881 cataattacc caccatagga taggtaaatg taatgatttg accgttaaaa ctttgatcag
    11941 tgatagtttc ttgataacca gtcatacttg ttgtaaaaac gatttctccg aagacactag
    12001 catctgcacc aaaagccttt ccttcgaaaa ctgttccatc ttctaatata agcaaacgtt
    12061 ccatactttt attctccctt ctgccacaca agttttccgt tgacaaaagt catttgtgtt
    12121 tcgcctttta ctttccagcc gataaaaggt gtattctccc cttttgataa aaagtcttct
    12181 ttatcgatcg tgcaagtttg tgtaatatca aaaactgcta catcagcggg ggcaccaacc
    12241 gttagcgttc ctgcattcaa accaaaaatc tcagccggtt taactgccaa ccaattgatc
    12301 acttgttcca acgtgaatcg accagtttct acaaaatgag tatagatcag ttgaaacgct
    12361 gtttcgcttc ctacgatacc aaatggtgat ttcatgaaac tttggctttt ttcttctaat
    12421 ccatgggggg catggtctgt agcgatgcaa tcgatcgtac cgtcaagcaa gccttcgatc
    12481 aaagcttctc tgtcctcccg tcctcttagc ggcgggttca ttttccaaaa gccgaaatct
    12541 tcgggaatat cttcgtcgat caaaataaga tgatggggtg aaacttctgc tgttacgtgg
    12601 attcctgctt tttttgcgtc tctgattaca cggacacttt ctttagtaga aacatggcaa
    12661 acatggtaat gaacaccagt tgcttcagct agcaacagat ctctagcaat ttgagaagac
    12721 tcggttacgc ttaagatccc gggaagacca agttcttctg ccttcttgcc agcgtgcata
    12781 acgccgccaa ataataaaga ttcatcttct gtatgggcta ctagggcttt attgtttttt
    12841 gcagcttctt tcatagccag atacatcgtc ccagctgttt ggacacctac accgtcattt
    12901 gtgaaagcaa aagcaccttc ttcaatcagt gcttcttgat caactaattt ctcacttcgc
    12961 agattttctg tgattggcgc atactgcaat accttcacaa ctgcatcttt tctaatgatg
    13021 tcatatactt gtctcagctt ttctgccgta tcaggtacag gatttaaatt cggcatcgcg
    13081 catacagtag tgaaaccgcc acgagcagct gagcgagtcc cagcttcgat cgtctccttg
    13141 tatgtaaatc ctggctctct caaatgaaca tggacgtcca ctaaaccagg tgtgatcaat
    13201 tgtccctttg catcaaacac ttcatcaaat tcggcttctg agaatcctgt accgattgct
    13261 ttgattcgcc catcttctat ccaaatttct gctggtgtcg tcatattttt tcgtgtattg
    13321 atctgaccgt ttttaatgag tgttttcatt ttttcatcct cctataatta cgcttttcca
    13381 tttagcactg cttctaagat tgccattctg acgaatactc cattgctcat ttgctgaacg
    13441 atccgcgact gtaatccttc cactaatgaa tcagctaatt ctacatcacg gttaactggt
    13501 gctgggtgca taatgattgc atgtttttgc aacttcttcg cacgctcaac tgttaaccca
    13561 tgttcctgat ggtagccttc ttttgagaaa ctttcttttc catcatggcg ttcgtgttgc
    13621 acccgaagca tcatcatgac atcaacttga tctagtagtt catccaatgg catataatgt
    13681 ccgtacactt cgaattcatc gtcgtaccat tctctaggac ctgagaagaa tacttgtgcg
    13741 cctagacgtt ttaacatctg catattggat ttagctacgc gagagtgagt gatatcacct
    13801 acgatggcaa ctttcagacc ttcaaaatga ccaaattctt cataaatggt catcaaatca
    13861 agcaagcatt gggttggatg ctgtccgctt ccgtctcctc cattgataat cgaacactgg
    13921 attgttttac tttgtatcag ttcgtcataa tagttctcat caccatgacg gataaccgct
    13981 acgtctacgc ctaatgctga catcgtcagt actgtatcgt atagtgtttc ccctttttgg
    14041 acagagcttg tgcttgcttc aaattcaatc acttctagcc ctagtttttt ctccgctaca
    14101 tcgaagcttt tatgtgtgcg agtactattt tcgaaaaaga ggtttgtagc aaagtactgg
    14161 cgcttttgcg gagtccaatt agcaccttgc ttgaattcct gccctctgcg gatcaagccc
    14221 ataacttctt gatcagtaag tgcctcaacg gtcaataaat gctttaagct gatacgttct
    14281 gatttgatga tcataatgtt tcctccttag ttttcttcgc ttcgtgaagt ttctggtaag
    14341 ataagattta aaatgatgcc aagtacagtt gctaatgcca ttgccgacaa tgtaaatgtg
    14401 cctacttcaa agacgagtcc gccaattcct actactaaga taactgaagc aatcaacaga
    14461 tttttctttt tatcaaaatc gattttgttc tcgattaaaa tcttcattcc acttgcggca
    14521 attactccaa ataagataaa actgattcct gagatgactg gtccagggat acttaaaatc
    14581 aaagcgctta attttcctac gaatcctaaa atgactgcaa atactgcagc tccaccgatc
    14641 acaaacacgc tatggacacg tgtgatagcc agaacaccaa tgttttcacc gtaacttgtt
    14701 acaggaggac ctccgacaaa ggctgccacg atttgtgcta atccatcacc tgtcaacgtc
    14761 ttatgaagac ccggttcttc aaagaaatta cgtttagtta gtttattcaa taccataaga
    14821 tggccgatat gctctgtcat cgtcacaaaa gcgatgggtg ccattgttgt gattgctcca
    14881 agatatagct ttggttcata ttgtacaaag ggaatctcaa agttaggaac agcaaaccag
    14941 gcagcgtcta cgattggctt agtatccacg attccaaaca gcaaggctac gatgtatccg
    15001 ctaataatac ctagtaaaat cggaatcaat ccgagaaaac ctcttagcca catgttgaag
    15061 aaaattgtta atcctaatgt gatcagcgct acagcaatat acttgaaatc atacgtgccc
    15121 tgattataca tagcatttgt tgctgcattt gccgcaagtc ccaacccgat aaccatgatg
    15181 actggaccga cgacgattgg aggcaagatt ttatccagcc atgctgaacc gatctttttg
    15241 atgatcagcg caacaatcaa atagactaat ccagttgtga ctgctccttg tgcaatcgct
    15301 ggataaccat ctgttttcat caaaagctgc atcgctgcaa tgaaagcgaa gctgcttcct
    15361 aagtacgcag gtatctttcc tttagtagta aataagtaca ccaatgtacc tagtcctgaa
    15421 ctgactagcg caatacctgg atcgatacct actaagatgg ggactaacac ggttgctccg
    15481 aacatcgtga acaagtgttg taaacttaat cctacccaat gtcctggggt tgggcgatcg
    15541 tggatatcta acaccacgtc atcattatga aatccttttt tctccgacat gtttctctct
    15601 cctctaatct tcctctttca aaatcatgat gcgatcttgt ccatcaagct cttgcatctc
    15661 tactaaaatc tcttccgccc gagatgttgg aatgttcttc ccaacataat ctgcacggat
    15721 tggcaattct cgatgcccac gatcgaccag gactgctaat gaaatttttc ttggtctgcc
    15781 aaaatccatt actgcgtcca ttgctgctcg aatcgttcgt cctgtataca aaacatcatc
    15841 aatcaaaata acttcttttc cttctaagga aactgggatg tcagaagaat gaagttccgg
    15901 ttcttctgga ttttcttttt tgtcatctcg gtataaagtg atatccagtt ctcctacagg
    15961 aatatcgata tcttctagct gcttcaggcg ttcggcaatt cttgatgcaa tgtagattcc
    16021 tcgtgttttg atacctacca atacgatatc ttggatacta tggtttctct cgataatctc
    16081 ataagtaatt cgagtcaatg ctcgcttcat ggttacctga tctactactt cttttgcttg
    16141 cattttcatt ccccctactt ttttgcataa aaaaactcct gccctaagag gacaggagga
    16201 atgtttagac tgagcgtata ctgatacact gatagctata ctatgtcaat gagcaatcta
    16261 cttatggcgc accatacgta tccttgctcc cacccttctt agcctcacgg gactaaactt
    16321 aaaggaattc tattaagttt tatgctttcg ttgttaatta ctataccgtt atctaaatta
    16381 aaatgcaatg ttttttctta atttttctaa agttttttca aaaacttctg gaagaggtgc
    16441 ttcaaagatc atcttttcgt ttgtagtcgg gtgcgtaaaa cctaacattt gcgcatggag
    16501 aaactgacca ttgcctttca aagttttctt cggtccgtat actggatctc cagctaacgg
    16561 ataaccgata tacttcatat gcacacggat ctggtgtgtt cttccggttt ctagttttaa
    16621 ttcaaccaga gtaaatcctt cgaaacgttc caatacagta aaatgtgtga cagccgactt
    16681 cccatcttct cttatcgcct gcattttccg gttttccttc gaacggccga ttggcgcatt
    16741 gatggttcct ttttcgtgag gaatctctcc atggactaaa gcaacatatt tgcgcaaaga
    16801 ggtcttatct tttaattgct gagctaatga ttcgtgtgcc tggtcatttt tggcaaccat
    16861 tagcagtccg gaagtatctt tgtcgattcg gtgaacaata cctggacgaa taacgccgtt
    16921 gatcgtagaa agatccttga tatggtacat cagcgcattg accattgtgc catgcgtatg
    16981 acctgctgaa ggatgaacga ccattccttg aggtttgttt atcaccgcta catcttcatc
    17041 ttcgtaaacg atcgtcaacg ggatattttc tgggaccaca tctaatgttt ctggttcggg
    17101 gatcaagaca gtaatctcgt ctccttgttt tactttgtaa tttgctttga ctggctgtcc
    17161 attgacagaa accgctccat ttttcagcca cagctgaatt tgcgaacgac tgtaatcggt
    17221 gaataaatta gtcaatactt tatcgatcct accagattca tggtgaatcg tcgttttaag
    17281 ttctgtcatt tttttctttg atccttttca tctaaaataa tataaattaa catacaaatc
    17341 actccacata caagtgtcac atctgctacg ttaaaaatcg gaaagctcat aaaatctgtc
    17401 tggaacatat ctactacata gccaagacga atacggtcga taaaattgcc aatcgcacca
    17461 gctagaatca aactcaatcc aatcatgaac cagcgattac ctttttcacg atttttaatc
    17521 atcaatgtga gaatcacaac gactgcaatg agtgtcacta tagtaaagaa ccacatcttc
    17581 ccttctagca tactccatgc ggctccagtg tttcgaatat gattcagtga caaaatacct
    17641 ggtataagat ttttcgtctc tccaagcgct agattatcag tgatccacca tttgaccaac
    17701 tgatccactc ctactagcaa agcgctgaat atccaataaa taattaacaa aaaatcaatc
    17761 cttttctatt tgtttataaa tatcacgagg tcgtacgatc ccgagcagat taccatcggg
    17821 attcccatct ttagtgataa gtacggcttc caaagtccct tgctcacgaa acatcgcttc
    17881 aacctcgtaa atatgtgttt tagctggaac aaaatgataa tttgtgtact ttccgttttg
    17941 gatgatcaaa tccaacgctc gtttatttgt taaatcaacc ggaccttttt ggctttcttt
    18001 tgctagccaa aacccgatat tacgtaaagt aatcaatgct acaaattttc ctttattgta
    18061 taacggaaat tgtgaatacc ttttttctgc tactatcttt aacagttcaa gtaacggaag
    18121 atcttgttca aaacctgtta cacgttttgc aaatcgaggc agcacggttt caggctgaat
    18181 cagttcttgt tcgatcagct ggattcgctt gactgtccaa tcatttggct ctgcaatgac
    18241 aaaatcgact gcgatccgat catggacgat cgcattcctt aactgagcaa gctgtaaaag
    18301 atcatcttcg tattttctga tcattaattg ttttctttga gctaggcgtc tgacaagttc
    18361 ggtaaatccc atatttcgtg catttcccat ctcgtctctc atccattttt cgattcgatt
    18421 gaaactgatt aaaaaaagat cagagtttgc cataattttc ctccttgatc aaatatgccc
    18481 ggttttttat atagaaaaag aacgcaatca acacaataac tgcaaaaaaa ctgaaaaata
    18541 aattttgtag gaaaacggcc cccaacccag ttgctagaaa taaaaacgga tagatttgaa
    18601 gaatcaagta attcattgtg atcacataag caggctttgc ttctttcact gtataaacgg
    18661 ggctttttag ccaataaaag gtgacctgca tcgtaaaggg cacttccaga tcaagcgtta
    18721 tcttttgttc atgactgaga acactgcgtt tttcattgtt gattcttagc tgaatcgagc
    18781 tacccatacc gtaaaaaccg gtttttctta taaatgtcgt tttcatcata ccctcctatt
    18841 atgctttagt atagctcaaa atacaataaa gaaaaagaat gatcctagtg taaacaaaat
    18901 taattcccta tcgtctaaaa aaagaagctg tagtcaaaat acagctcctt cgatggtaga
    18961 ttataatcaa gcttaaaaca atcccaacac ttctccgtca tttgtcacgt ccatattcaa
    19021 tgcagcaggt tttttaggca aaccaggcat cgtcataaca tctccagtca aggctactaa
    19081 aaatcctgcc cctaattttg ggatgatttc gcggatcgtg atggtgaaat cttccggtgc
    19141 acctagcagt gaaggatcat cagaaaatga atactgtgtt tttgccatac aaatcggcag
    19201 tttgtcccat ccattcttcg taaactcttc tatttgtttt tgcgcttttt tgctgaaaac
    19261 aacaccattt cctccgtaaa ttttcttgac gattgtttct gtcttttctt gtatcgtttc
    19321 ttctaggcgg tataatggct tgtaattagc ttcttttcta tcaatcaatc gtactacagc
    19381 ttttgctaaa tcaacgccgc cttctgctcc atctgcccat acactcgctc tcttggcaag
    19441 aatcccctga ttttcacaca agtgctccaa cagtgtgagt tcattgtctg tatctgttac
    19501 gaattcattg attgcgacga tcacaggtag ctgatattgt tccatattac gtatatggcg
    19561 ctttaaattt gcaaaaccta ctttcaaagc atcaagattc tcatttttaa gttcatcttt
    19621 tttcattccg ccatgcattt tcaatgcacg gacagtggcg acaatcacca cagcatccgg
    19681 cgcttttttc aagttaggta ctttgatatc taaaaatttc tctccgccaa gatctgcacc
    19741 aaatcctgcc tctgtgacaa cgtaatctgc taatctcaat gctgtcttgg tagctaaaat
    19801 actattgcag ccatgagcga tattggcaaa cggtcccccg tggacaaaag ctggtgttcc
    19861 gtaaattgtt tgtacaagat tcggcttgat tgcatctttc agcagtaatg ccagagcgcc
    19921 ttctgctttc aagtctccag cagtcacagg ctgacggtca aatgtatagc cgacaacgat
    19981 ccttgcaaga cgagccttta aatcttcaag gtctgccgca agacacaaga tagccatgat
    20041 ttcgctggca actgtgatat caaacccatc ttctcttgga acaccttgga ttggcccacc
    20101 caagccaaca attactttac gcagttcacg atcattgaga tcgactaccc gtttccaaat
    20161 cactcggcgg gcatcgatgt tcaattcatt tccttgatga atatgattat ctaaaagagc
    20221 agataatgca ttattagctg ttgtgatagc atgcatgtca ccagtaaaat gcaagttgat
    20281 atcttccata ggcaatacct gtgcatatcc tccgcctgca gcaccacctt tgattcccat
    20341 cacaggacca agtgagggtt ctcgcaatgc aataacagat tttttattga ttttattgag
    20401 cgcgtctcct aaccctacag tgatcgttga ctttccttcg ccagctggtg ttggattgat
    20461 ggaagtaact aagatcaaat gcccctcagc gttatccttt gatcgattga ttccagaaaa
    20521 attgattttc gccttatatt tcccatacag ttccaaatcg tcttccttaa gatctaattt
    20581 actagccact tcaacaatcg gttctagctc tgtttcttgt gaaatttgca gatcattctt
    20641 catttgatta ccccttttca cgaactttat tcaacaaaat atcttctttg ttaagatatg
    20701 ccaattatac ctttttttat tcagaaaaaa aacataaaat tacaggtttt taatttattt
    20761 gacgtactta taaatactct ttataatgaa ggctatgatc agactttaga aaggtcgtga
    20821 gtgctttgac cacaggaatt gtaaaatggt ttgacaacaa aaaaggatac ggctttatca
    20881 gttatgatga cacagaagaa atttttgtac attttactgc gattgaagaa gagggattca
    20941 aaactttgga agaaaatcaa gtggtcgaat ttgagatcat cgaagggaat cgaggaacac
    21001 aagctgccca tgtcaagaaa ggcaacgttc agactaccga tactagtatg catgaataga
    21061 taaacaaata gagtgagacg ctaaacggga tgaaccgtac gtctcactct tttcttttcc
    21121 tgtaaaaatt cgttttttta ggttctgatt tttaaaatta gctttttctt tttttcgtat
    21181 aaggataagg aattgattct gatttgggtc cgtttttttc aaaatgatca aaaaggacta
    21241 cttgatcttt ttttcgcatc atgaaaacag atggggcttt ctcatcttgc aacatcattt
    21301 gcaccccagt attagttggc gtcaaagatc gaatctgtcc gacagatatt tctcggtctt
    21361 tccaaaaagc agcataacgt ataatcacag agtttgattt taaaataaaa gaacgccgca
    21421 aacctagaaa tagaaaaaca agaaaaatac ctaaaacaaa attgcttttc caataaggac
    21481 ctgtgttttc taaagaaaga atcagactaa aaaacagaat gatcaaggtc aatgaccaat
    21541 agatgatcgt atgtgccagt tcaggctgcc atcgaatatt ttttttcatt ctctaacctc
    21601 tcaccgattc agtaatgata taatcttacc ataacattag tttgaacaaa aaggagaatc
    21661 tctttttatg ttaaaagtat acattgatgc ctctacaaaa ggcaaccctg gtcctagtgg
    21721 aggtggaatc ttgattgtct atcaaggtaa acaagaacag ctatctatcc cgctttctat
    21781 agggacaaac catcaagcag agttcgaagt ctttttaaaa actttagaat ttctaaagaa
    21841 aaacgactta cagaatgaaa cgattttctg tttttcagac agcaaaacac tagtttctac
    21901 ggtcgacaaa aacgcaacga agaacgagct cttccttccc taccttcaaa gaatccaaga
    21961 acttctatcc gaattttctt tattgatcct gcaatggttg cccgaaaaca aaaataaagg
    22021 agcagataat cttgcacgtc aggctctcca taaacaatta aaagaaaaaa agactcttta
    22081 aattaaaaac tttaaggagt ctttttatag tttctcattt aatataaaag ataattgtga
    22141 tcaaacgtaa tttccaattg gtaatctccg attttttctt ctatttgact acgtaagatt
    22201 ctttctagtt ctgtatcaac aaactggaat ttctcaggga ccactacatc aaattgcagc
    22261 gtttttcctt gatgaattac tctaaaatca tgaatattaa gccctgtacc tgtttcaaga
    22321 agaatttgct ttaactgccg caagatatta tagtattttt cgttatcaac aggcattgga
    22381 tccaagtgac agacaagttc cacatccaaa gactttttaa agtctttttc tatcagatca
    22441 ataatcttat gagccacatt caaattcatt gattcatcta cttcgatgtg gactgatgca
    22501 aagcgtttat tgggtccata gttatgcacc aataaatcat gatagccaag tatatcatcg
    22561 caagcattta aatgctcttc catttcttca atctctgctg gcgttgggcg acttcctaat
    22621 agttcgtata caaaatttcg aagcatcgtc aatccactat aaaggatata taatgctaaa
    22681 gcaaaaccta tatatccatc aatacgccat ccagttaccc attcgattgc tgctgaaagc
    22741 aatacagcca gtgtagtaaa gacatcatta taactatctg ttgctgtagc tctcaatgtt
    22801 tcagaatcaa tggattgcgc aataacctga tacattttgc cttgtgcaaa tttcaaaaag
    22861 atggaaatga ttaggacaaa aaagacgatg ggtgttaaag agacattttc tggatggatg
    22921 attttttcag cagaagactc taaaaattga aatcctacat aagtaatgat gatcgaaaca
    22981 aataacccac tgatatattc gaagcgttcg tgtccataag gatgttcctg atctgctggc
    23041 tttgcagcaa ttttaaatcc gatcaaagtc aatatggatg aagcagtatc tgatagactg
    23101 ttcactgcat cagccataat ggagacactt cctgaaaaaa gcccgatcat tagcttagag
    23161 acaaacaaga tcagattcgt aaccaatcct aagatacccg ctagtactcc tttttttgtc
    23221 cgctgttgcg gtatttgttt ctttttttct tcttgtttct tagccaagtt ttttacccct
    23281 tctagtttgc ttgttgctca ggtaaaaaga tggagaggac gattgttttc ctctcctctc
    23341 ttcctttttc tatctatcgt gcgtcctatt actattaaaa gatggtcatt attctggttt
    23401 caacggtgtt gctgcaaata acgcttttgc tagtaatggt gttatgattc ccgcacctat
    23461 tgcttctggt atcccattga ttgctgcaac ccctgctaag gttttaaata aaagagcaga
    23521 gtctacacca taggcatctg ccgttgcacc tgtataaaat aatcccatga atcctaacac
    23581 taagactgta ttgaccaatg aacccagtac ccctgcaaag gctgagctga tcgttattga
    23641 tttatttttt tgataaatcc agtggaaaac caagcctgct actaagccaa caagtactct
    23701 gggaactacc gagataatcg gattcgtgaa cactaatgta tcaaatggcg ttgttggcat
    23761 ggcataggca cgaacaattg tagcgattcc ccaaaacaag ccaatcacca tgccatcttt
    23821 tgggcctaat actactgcag caataattac tgtgatatgg ataattgtta aactaatgaa
    23881 tcctaatgga ataaacccca gccaaggaac catcgcttgt acgatgatga ttgctaacaa
    23941 gatcgcacgt aagactagtc gaaaggtctt atttctacta ttcatttttt cacccctttt
    24001 tatttatact aatcgtaaaa atttctgtca ctttcgtcaa ctaaagacca tccgaatccg
    24061 accatttgat tagatgcgcc atccaattga aaaaagtatg agggcaagac ttgtttgtct
    24121 tcgcccccat aatcatgttt cgttcaatgt ttgaactttt gtttttctgt caaacgtaaa
    24181 cgccctattg ttgcgcaacc cattctttcg cagctttaag tgcatctgga ataccagcag
    24241 gatttttccc gccagcttgt gccatgtctg gacgtccacc accgccaccg cctactttcg
    24301 gtgcgattgc tttaatcaag tcgccagctt ttagtccagc ttcatttgat tctttcgtca
    24361 ttgccgctag cagactgact tttccttctt gttcgtttgc taaaacaaga atatctgaaa
    24421 tatttttttg tttccattgg tcagctaatt gacgcagctg attcatatct ttcactttta
    24481 cttgtgctgc aatgattgtt tttccattga tcgtttctgg atctttgaat acatcaccag
    24541 cttgttgatt cgctaacttg ctagctaatt gttcattttc cttttgtagc tctcttactt
    24601 gctgttgcaa ttgttccgct tttgaaacaa cttctttcaa ttgaggtgat ttaacaattg
    24661 ttgctacttg ttttagttgt ttttcttctt cttcaagtag ttcgtacgct tctttacttg
    24721 ttacagcctc gattcgtcgc acgcctgcac caattcctga ttcagaaacg attttgaaga
    24781 ttccgatgtt ttctgtattt gctacatgtg tgcctccgca cagttcgatt gaccagccgc
    24841 caatatttac tactcgaacg tcatgtccgt atttttcacc aaacaaagcc attgcaccca
    24901 tgtttttggc tgtatcgata tctgtttcaa ttgtttcaac tgggatagct tcccagattt
    24961 tttcgttgac gattctttcc atttcagcta attcatctga agtgatttga ccaaagtggg
    25021 taaagtcgaa tcgtaaatgt ccaggagcga ctaaagaacc agcttgattt gcatgctcgc
    25081 caagtacttc tttcaatgcc ttgtgcagta gatgggttgc tgtatggttt ttgatgatcc
    25141 gattgcgcat cgattgatcg atcaatagat gataggtttg tccttcactc attggtgctt
    25201 ttacttctac ggtgtgcata aattgaccat taggcgcttt ttgaacatct gtaacaactg
    25261 ctacgatttc gcctacttca ttcatgatcc atccttgatc cgctacttgc ccacccattt
    25321 ctgcatagaa aggtgtttga tcaaagatca tttgagcttc accttctgcg acttcattca
    25381 aaagagactc acctttaacg atgaccaata attgactatc tgattcttgt tctgtgtatc
    25441 caatgaattt actttctact ttgatatctg tcaataaagc agattgtacc cccattgatt
    25501 tttcagtact gcgggcagag cgagcacgtt cgcgttgtgc atccatttct ttttcaaaac
    25561 cagcatgatc aactttcagt ccttcatctt ctgccacctc ttccgttaat tctaccggga
    25621 agccatatgt gtcgtatagt ttgaagatgt cttttcctgc tagagtcgtc ccgttttcgt
    25681 tttttacttc ttggatcact tgatttaaga tttccaaacc ttcattgatt gtttcattga
    25741 aacgttcttc ttctgtacgg ataacttttt caataaattc tttttgttga agtacttcag
    25801 gataataact taccatgatt tccccaacaa cagggactaa acgatataag aatgcttgtt
    25861 caatacctaa ttttttccca tgcatgacag cacgacgaag caaacggcgt aagacatacc
    25921 ctctcccttc attcgatggc aatgcaccgt cgccaatagc aaatgaaagg gcgcgaatat
    25981 gatctgcgat tactttgtaa gaaatatctg ttactgggtt ttcaccatat tttacttgtt
    26041 tgcttagctt ttctacggcg tggataatag gcataaacaa gtctgtctca aagttcgttg
    26101 acgcatcttg aacgattgag accatgcgtt ccaatcccat ccccgtatca atgtttttat
    26161 gaggaagtgg ttcaaatgtg ttatcaggct tatggttgaa ttctgaaaat actaagttcc
    26221 agatttctaa ataacgttca ttttctcctc ctggatagtt ttcaggatcg tcttctgcta
    26281 aatcgttata agcttctcca cggtcataga aaatttccgt atccggacca catgggcctg
    26341 caccgatatc ccagaagttg tcttctacat caataatgtg atctagtggc aggccaactt
    26401 cttcatgcca gatgcgtttt gcttctgtat cttttggata aactgttaca taaagttttt
    26461 ctggatcgaa tgccatccat tttggatcag tcaaaaattc ccatgcccag tggatggctt
    26521 catttttgaa atagtcacca atcgaaaaat tacccagcat ctcaaacatc gtatggtgcc
    26581 gtgctgtttt accgacattt tcgatatcat ttgttcgaat agatttttgg gcatttgtga
    26641 ttcttggatt ttctggcaca actgaaccat caaaatattt tttcaatgtt gcaacaccag
    26701 agttgatcca caataaggtt ggatcgttca cggggatcaa agacgcacta ggttcaatcg
    26761 tatgtccttt agattggaag aaatcaagaa acatttgtcg aacttgtgca ctacttaatt
    26821 gtttcatgtc attacctgct ttctataaat taaatacaaa aaaacaccat cacagccaag
    26881 gacgaaaact cgcggtacca ccttgattgc aatgatgtgt aatcattacc tcttaaactc
    26941 gataacgctg agctgcgtcg gtatttctac caaaatagaa aaagggagca atttgattag
    27001 tgactattct tttttcagcc aagaaagaat tttctgtgag tcttaaaaaa caaatcatat
    27061 ccttttcgta cttaattagt atacgcattc tattgatcac tgtcaatatc tcaattttta
    27121 ttttcatttc cttttggtca tactccacga tttttggtac gataagaata aaggaatcaa
    27181 tacatctctg ctattcatga caaggaggaa tatgatgaaa aaaatagttg gagcggatga
    27241 atggatacgc gcagctagct gctttttgag atatcaagtt tttgtattag aacaagggat
    27301 tcctgcagaa ctagaattcg acggaaaaga tatcccgcaa caaacctatt atctatttct
    27361 tgatcaaaaa agaccagtag ctactttgcg ttatgaaaag aaaacaccta cttgcttcaa
    27421 gccggaacga ttttgcgtag caaaagaata ccgaggaaaa gggttaggcg gtcagttgct
    27481 tgaagctgct gaaaaacggg cggtttcaga aggttgtcag actagttatg tagtagctga
    27541 aatgacggct gtagattttt atgaaaaaca agggtatcat acttgcacag aaccttttat
    27601 tgaagatgaa gtactttgta tcggaatgaa aaaaagtcta gcttaaaaac aagagattgt
    27661 gacacgcctt ttgtcacaac ctcttgtttt ttatttatgt gaatttttct tatttttacg
    27721 agcagtacgg ctttgctgtc tgcgttcgat tttgcgttct tttttatttt tttctgcaat
    27781 tgcccaatcg atttttttct tataaccagg cttgatcttt ttctttttct tcttcacaag
    27841 accaatcagc gtgggatcta atacatcttt tgatttttct cgtttggcac gtctattacg
    27901 atcgtatgtt ttgacgattt ccccgttttt gatttctttt ggttcaaatg agacgcccaa
    27961 tttttctatc gcatcaatgg cttgctcatc atttggcgaa tataacgtaa tggccgttcc
    28021 ttttaagcca ttacgtccag tacgtccgac acgatggata aagaagtcta gatcttctgg
    28081 aatttctgca ttaataacat gagagacacc ttcgatatcg atccctctag cagctaaatc
    28141 agtcgctaca acatattgat actccagatt ctgtacttgt cgcatcactc gttttctctc
    28201 tcttggggtg atgtctccat gaatctttgc tactttcaag ccttggtttt tcaagtaatc
    28261 ggcaatttca tccacatgct gttttgtatt ggcaaatacg attgctaaat aaggatgacc
    28321 aattgtcagc agttgataaa tcatttgatt ctcatcttta ccttttgttg aaatcagcca
    28381 attttcaatg tcttcagaaa taactgcttt tggtttgatt tcttcaatga ttggattttc
    28441 catgtatttt cgtaagaatg gacgaagttt ttctggaata gtggcagaaa aaactaaaaa
    28501 ctgcagcttt tctggtaatc gacttgcaat ttggtctact tcatttaaaa aacccatatc
    28561 caatgtcata tccgcttcat cgacaacaaa tgcaaatgcg gtgtgaatct tcagcgcttg
    28621 ttcattcatc atgtctaata ttctaccagg cgtgcctact acaacctgtg gctgttggtg
    28681 atgcagacga tttaattgac gctgtttgtc tgtaccgccg acaaaattcg agacacggat
    28741 ctccggtgta gaaaatttag cgatttgtgt agctgcttca tagatctgtg cagctagttc
    28801 cctacttgga gctgtaataa caacttgtac ttcctctttt tctgcattga ttttttcaaa
    28861 caaaggcaac aaaaacgtat gtgtttttcc tgtaccagtt tgagattgcc caatgatatt
    28921 ttttcctttt gcaattaaag gaatcaaacg ttcctggacc tctgtgggct gtttgaagcc
    28981 ctttgcggtt aacgcttcat tgataaacgg tttgaagcgg aatatatcaa aattattcat
    29041 tcgtgcacct cttctcttta aattatcacg actttggcat tataacacag acaaagcgtt
    29101 tttgctcagc taatttcgag aatcatcatc ctttgtttgg ccaatcgtgc taacagtata
    29161 tgtgtgacta gctttttcat aaaacagaat ccgttttttt ctcaaaaatg atagcaagat
    29221 atttaaaata aaaatacctt gtagtaacaa gacaccgaga gaaatgaata ctgcactcat
    29281 ctgcaacacc gtattcgttg tcgaattcat gatttgctga agcaactgcc ccaaccagct
    29341 taaaccataa aaaagaaagt acagataccc gtagcggatc atcaatgctt ttaaagaaat
    29401 tcgttcgcgg tcttcttcca ccaaacggat acgcacaatt tttttgccaa gtgtccatcc
    29461 attggttata aacggtacaa tcataaagta tacccctact tctaataaaa gccaccagat
    29521 atgagaagta actgcccctg aatcgaacgc aattttaaag aaaatcgccg ctaccaaatt
    29581 aataagagat aaaaatgtcc agtcgatcaa ataagcgacc aagcgtcgag tcaatgagac
    29641 agtctttcct ttttcataag aaagttcatc cattcgtctt cttgtaggaa gcataaatgt
    29701 aaataacggc gtaacgacaa aaccaatgat tccacccaat gtatttgtca ctaaatcatt
    29761 cacatcaaat aagcggtatg gtcttgggta gataaaataa agtccactta gctgcgtcaa
    29821 ttcaaaaaat agagaaaggc agaaactagc aatcatcgtt ttcataaaag aaagccggaa
    29881 ataatatctt aaataaatgc caaacggtat gagtaataaa acattaaaag ctggttccaa
    29941 aaatacactc tgcttcattg ccggcaggta ggtactaggt tcagacaaga ccaataccgt
    30001 gttgtctctg aaatgttgcc atgaagcaaa taaatgcagc tccatctttg gccctgtcat
    30061 ttgggcaacc gtctcctttg ccggaagtgg taagatcacc aaaaaatacg cacacaataa
    30121 ataaaaaacg aaagaataca aaataactgc ccttgttact agaaatgttc catactttcg
    30181 atattcataa ataaaaaaaa cagatgagat cgctaaggct aaaaatggaa aaacaatcag
    30241 cgcaaaacga atcggttcga tataacttgc catatgactc ctccttgatt ctttgtatct
    30301 ctcttatctt agcagtcctt tgctgtttca agcaagtaac gaggctatta tttctcattt
    30361 ctttcaaagt aaagtaagat aaacacaaag ggggtgtcga gatgctgaat atgtactttg
    30421 tttttggcgt acctattttt ttactgttcc tttatgctac gatcgcttat gtaagaaaaa
    30481 gaactacaat tcattattta ggatttattc tcctgattat ttcgggtttc atgctagtct
    30541 tcaatcttca gacttggcag caagcacttc ttgaaatgga taaaatgact ccacatgctt
    30601 tgtctaaagt gcttggctac cctgtctatc tgatttggct tcccattttc atttctggtt
    30661 gcttggtttt attaaatatt tatcgcggcg tacgccgaat agttcagtta cgcaaatcaa
    30721 agtaaaagac agaaatcggt tgagcccgat ttctgtcttt tactttgcta tttcatcgta
    30781 gacaaaacga catttacgtg atcaaaatta acgagtactt ttgacatttc ttccacgtac
    30841 tcctcattta aataaaatat tttgtttggc agagaagtgc agttctaata aaattcgaat
    30901 gttttagcta tctacgtttt aaatagagat aaatcgggat accagcaagt gtcaatacta
    30961 tccccatcga agctaaaaag gtctgagtga taagcgtgct tgatacgata aaagtccctc
    31021 caatgattgc tactagtgga ataaacggat acattggaac tttgtatggt cgaaacaaat
    31081 caggttcttt tttacgaaga agaatgacac caacaaagac catcgtataa aaaatccaaa
    31141 tgacaaagat cagcatatcc gttagtgtat caaaaccacc gatcatcatc atgccaatag
    31201 cgatgactag ctctaagata cctgcggcta caggaacttt agttttatca tgaagtttag
    31261 caaagagctt gctaaaaggc aggttgtttt cttttgccat aacataaggg agacgcattc
    31321 cagtaagggt atagccattg atggttccat aaacagaaat caagatacca actgtgacca
    31381 gtcttccacc gaagccgcca aaaatcattt ttgcaacgtc acttgccgca ttgctgtttc
    31441 ctgcgactcc gtcgatagat gcggttctta agaacaccgc atttactaat aaataaacga
    31501 tcatgattcc gatgattcct aaagaaattg cttttggcaa atctttcgcc ggctttttca
    31561 attctcccga aatatttcct acgtgtatcc aaccgtcgta tgcgaacatc gttgctagca
    31621 agcccgctcc caaggctgag aaaaatgaaa gattttctcc tgcttggatc ggaaacagtt
    31681 ggaagtctac tccttcttgt cggaacaaac cgaaaatgac gatcacaaac aaaggaataa
    31741 gcttgcagac aagcgtaatc gactgaaaag cccctccagc tttagaacct aaaaaattga
    31801 tcaacaggat cgatacagca gctgtaacag ctacgggaac aatcattgac tgggaaagac
    31861 caaatagatt gacgaactgt gtaccgaaaa tgatcgacaa ggctgctaca tttgctggaa
    31921 aataaatcac gacttgcgcc cagcccaata aaaaagctgc tgtatttccg taaatccgtt
    31981 cgatatattt aatcatccct cctgtttctg gaatagcagc tgccagttca gctcctgtta
    32041 gtcctgcaca gacagaaatc ataccaccga gaaaccaaga aaacatatgc aaactggcgg
    32101 atcctgtaac ttctgctacg cttgcggctt taaaaaatac ccctgcaccg atcactgtgc
    32161 ccataacagt cgataaagca gtaaaaaatc ccatcgtacg ttttaattca ttttctattt
    32221 ttacttcttt tactggcgtg gcttgatatt cttccatccc ttgtcccctt tcataccata
    32281 gccaatccca aaagtataaa aaactttttg gatgtggttc ttgttttata ccgtctctac
    32341 caatttttct ttcagttttt ccaacatatc ctttgtcatc acatctaagt cgtatttcgg
    32401 cgaaaagccc cattcctctt gcgcacaaga aacatctagt gaatttggcc aagattctgc
    32461 tattgactga cgtacaggat ccacatcata atccatttca aaatctggta tgattttttg
    32521 aatagatgct ttgatctctt ccgggtcaaa agacatcgcc gatatattga aggcatttcg
    32581 atgaaccaat cgatcaggat ctgcattcat gagttttata atggcatcga tcgcatctgg
    32641 catatacatc atatccattg atgtaccttg atcaatgaaa ctagtgtact ttccttcttt
    32701 gattgctgaa taataaatat cgactgcgta gtctgtcgta ccgcctcctg gcaaggttgc
    32761 ataagaaatc aatccaggga aacggacacc acgagtatct acgccatatt tcacataata
    32821 ataatcacat agtagttctc cagctacctt cgttacccca tacatggttg taggacgctg
    32881 aagagtatct tgtggggtat gatcttttgg agtatttgga ccaaacgcgc cgattgaact
    32941 tggcgtaaaa aatttcagct ggaattctct agcgacttcc aatgcattga ccagcccagt
    33001 cacgttcaaa ttccaggcta attgtggttt tgcttctgca acagctgaca aaagagcagc
    33061 caaatgaatc aatgtgtcca cttgatattg ttctgctatg ctgcgcattt tttcgtaatc
    33121 cattacatct aggatctcaa agatatcgtt tttctcaatg acttcaggca tgcgtatatc
    33181 tgtcgcaatg acattttcac tgcctagttc ttgacgtagc cgtgtgatca actctgaacc
    33241 aatttgtcct aaacatcctg tcaccaatac ttttttcatg atgatcctcc ctgatctttt
    33301 ttactactat cttcagatga ttaacaatat atgcgtgaac atgttctcta attttaaata
    33361 gacgaaaaaa ctaaatttag ttaaataatt tccatttctt ttcctatttt ttgataaact
    33421 gctactgctt catcaagcat ttccctcgta tgttccgcag tcggcatatt tctgatcctg
    33481 cctgtcccaa gaggcacagt tggataaacg attggtttag cataaatccc atgttcgatc
    33541 agcttcttag agaaagtctg agtcactttt tcgtctccca aaatgactgg agtaataggt
    33601 gtttcagaca taccgatgtt atagcctact tttttcaatt cttctttaaa ataattagca
    33661 ttttcccaaa ctttttcgac aagttccgga tgttcttgca tcaaagtaat cgaagccaat
    33721 gcagctgcag ctgcacctgg agttagcgaa gtagaaaata ggaatggccg acttctagct
    33781 ttcaaccagt cgattagtgt tttcgatcca gctacataac ccccaacaac acctatagct
    33841 ttcgataacg tgcccatttg catatcgatt ttgtcgctta atccaaaatg cttgacggtt
    33901 cctgcacctt ttccagttac gcctgagccg tgcgcatcat cgacgtaggt gatcagtcca
    33961 tatttttcaa caatcggaat aatttccggc aatcgtgcaa tgtctccatc catggagaaa
    34021 acaccatctg tgatatacat gattttttta tatttcttac tttctatcgc ttcctttgct
    34081 tttttctcaa ggtctttcat atcttgatgt ttaattcgga tgatttttgc tccggataag
    34141 cggcaaccgt caataataga tgcatggttt aattcatccg ataagatagc atcttctttt
    34201 gtcattactg cagagatagc gcccatattg cagttgaatc cagattgaaa ggcgattgct
    34261 gcttctgtcc ctttgaattc tgcgattttt tcttctaatt gctgatggat tttcagcgaa
    34321 ccattgatcg ttcggactgc tcctgctcct acaccataag tttcggttgc ttcaatacat
    34381 gcttttttca attcttcgcg attggcaaat ccaagataat tgttggatgc tagattgatc
    34441 atcttttttc cgtctacgat gatacaagca ccattttcac tttctagtat gtcgatcgta
    34501 ttgtataacc ccttttcttt cagttgatcc aattctttct ctaaatacat tgttagttct
    34561 ttggaagaca ttgtctccac ctccatattc atctgtcttg cagaacatac caaacatata
    34621 tcgtgtcatt catctacaaa gcaagtatgc tatatttttg ataacgctta cactgattat
    34681 gagaattatt taaccacatt ttttatttac agtatctttt tactttcaaa tgacttatat
    34741 agtatgaaca attacctttt agataatgat ctattttact ctacctgggc agataaatac
    34801 aacacaacca ttgatagaag ccctttcgtt aaaatcaatt gtattctgaa tatttaattt
    34861 atttattgca tagaccaata aaaatacata aaatttccat cttcgtttat cagcaatttt
    34921 taattttact ctctgaatat ttcttaaaag ttttacttgt tgaattcgaa aaaaggactg
    34981 tgacaaaagt cttgtcacag tcctattctt ccgaata
//
LOCUS       DAYYBH010000019        34840 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000019 DAYYBH010000000
VERSION     DAYYBH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.019"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACHAUG_001124"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACHAUG_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8480230.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3297)
                     /locus_tag="ACHAUG_001125"
     CDS             complement(2944..3297)
                     /locus_tag="ACHAUG_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480231.1"
                     /translation="MSCFVESERLSNGNNSVLNEYDKVVINKVKSLCEEARESISHIN
                     GSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKL
                     NNYKLIDYKYLSESN"
     gene            complement(3394..4599)
                     /locus_tag="ACHAUG_001126"
     CDS             complement(3394..4599)
                     /locus_tag="ACHAUG_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8480232.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5293..5679
                     /locus_tag="ACHAUG_001127"
     CDS             5293..5679
                     /locus_tag="ACHAUG_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8480233.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5777..5968
                     /locus_tag="ACHAUG_001128"
     CDS             5777..5968
                     /locus_tag="ACHAUG_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480234.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6482..6814
                     /locus_tag="ACHAUG_001129"
     CDS             6482..6814
                     /locus_tag="ACHAUG_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8480235.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6724..6927
                     /locus_tag="ACHAUG_001130"
     CDS             6724..6927
                     /locus_tag="ACHAUG_001130"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8480236.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            6958..7104
                     /locus_tag="ACHAUG_001131"
     CDS             6958..7104
                     /locus_tag="ACHAUG_001131"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480237.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            7111..7266
                     /locus_tag="ACHAUG_001132"
     CDS             7111..7266
                     /locus_tag="ACHAUG_001132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8480238.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7542..8882
                     /locus_tag="ACHAUG_001133"
     CDS             7542..8882
                     /locus_tag="ACHAUG_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8480239.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(9123..9530)
                     /locus_tag="ACHAUG_001134"
     CDS             complement(9123..9530)
                     /locus_tag="ACHAUG_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8480240.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(9719..10729)
                     /locus_tag="ACHAUG_001135"
     CDS             complement(9719..10729)
                     /locus_tag="ACHAUG_001135"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8480241.1"
                     /translation="MPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVNVDG
                     VLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPI
                     PIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEE
                     QDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSK
                     ILNTNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLT
                     DKNFPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKN
                     PN"
     gene            10855..11073
                     /locus_tag="ACHAUG_001136"
     CDS             10855..11073
                     /locus_tag="ACHAUG_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480242.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            11166..11729
                     /locus_tag="ACHAUG_001137"
     CDS             11166..11729
                     /locus_tag="ACHAUG_001137"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8480243.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11922..12161)
                     /locus_tag="ACHAUG_001138"
     CDS             complement(11922..12161)
                     /locus_tag="ACHAUG_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480244.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12185..13708)
                     /locus_tag="ACHAUG_001139"
     CDS             complement(12185..13708)
                     /locus_tag="ACHAUG_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8480245.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(13726..13848)
                     /locus_tag="ACHAUG_001140"
     CDS             complement(13726..13848)
                     /locus_tag="ACHAUG_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8480246.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(13878..14861)
                     /locus_tag="ACHAUG_001141"
     CDS             complement(13878..14861)
                     /locus_tag="ACHAUG_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8480247.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14885..15034)
                     /gene="rpmG"
                     /locus_tag="ACHAUG_001142"
     CDS             complement(14885..15034)
                     /gene="rpmG"
                     /locus_tag="ACHAUG_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8480248.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15055..15432)
                     /locus_tag="ACHAUG_001143"
     CDS             complement(15055..15432)
                     /locus_tag="ACHAUG_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480249.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15464..15643)
                     /gene="rpmF"
                     /locus_tag="ACHAUG_001144"
     CDS             complement(15464..15643)
                     /gene="rpmF"
                     /locus_tag="ACHAUG_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8480250.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15658..15927)
                     /gene="rpsN"
                     /locus_tag="ACHAUG_001145"
     CDS             complement(15658..15927)
                     /gene="rpsN"
                     /locus_tag="ACHAUG_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8480251.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16450..18216)
                     /locus_tag="ACHAUG_001146"
     CDS             complement(16450..18216)
                     /locus_tag="ACHAUG_001146"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480252.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18176..19930)
                     /locus_tag="ACHAUG_001147"
     CDS             complement(18176..19930)
                     /locus_tag="ACHAUG_001147"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480253.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20040..20609)
                     /locus_tag="ACHAUG_001148"
     CDS             complement(20040..20609)
                     /locus_tag="ACHAUG_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8480254.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20627..22042)
                     /locus_tag="ACHAUG_001149"
     CDS             complement(20627..22042)
                     /locus_tag="ACHAUG_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480255.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(22053..22721)
                     /locus_tag="ACHAUG_001150"
     CDS             complement(22053..22721)
                     /locus_tag="ACHAUG_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFM8480256.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22852..23436)
                     /locus_tag="ACHAUG_001151"
     CDS             complement(22852..23436)
                     /locus_tag="ACHAUG_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8480257.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23771..24001)
                     /locus_tag="ACHAUG_001152"
     CDS             complement(23771..24001)
                     /locus_tag="ACHAUG_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8480258.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(24016..24966)
                     /locus_tag="ACHAUG_001153"
     CDS             complement(24016..24966)
                     /locus_tag="ACHAUG_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8480259.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24963..25805)
                     /locus_tag="ACHAUG_001154"
     CDS             complement(24963..25805)
                     /locus_tag="ACHAUG_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8480260.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(25938..26663)
                     /locus_tag="ACHAUG_001155"
     CDS             complement(25938..26663)
                     /locus_tag="ACHAUG_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8480261.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(27124..27438)
                     /locus_tag="ACHAUG_001156"
     CDS             complement(27124..27438)
                     /locus_tag="ACHAUG_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8480262.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(27454..29049)
                     /locus_tag="ACHAUG_001157"
     CDS             complement(27454..29049)
                     /locus_tag="ACHAUG_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFM8480263.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(29072..29962)
                     /gene="iolE"
                     /locus_tag="ACHAUG_001158"
     CDS             complement(29072..29962)
                     /gene="iolE"
                     /locus_tag="ACHAUG_001158"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFM8480264.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(29979..31004)
                     /gene="iolG"
                     /locus_tag="ACHAUG_001159"
     CDS             complement(29979..31004)
                     /gene="iolG"
                     /locus_tag="ACHAUG_001159"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFM8480265.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(31028..32035)
                     /locus_tag="ACHAUG_001160"
     CDS             complement(31028..32035)
                     /locus_tag="ACHAUG_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8480266.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(32148..33308)
                     /locus_tag="ACHAUG_001161"
     CDS             complement(32148..33308)
                     /locus_tag="ACHAUG_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFM8480267.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(33384..34838)
                     /locus_tag="ACHAUG_001162"
     CDS             complement(33384..34838)
                     /locus_tag="ACHAUG_001162"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFM8480268.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT        10890 a         6944 c         5340 g        11666 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattact ctttcttaga ttaaacttca gagtattcaa
     2641 atagcggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc attcctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat taaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg cttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaattt tttgttaaaa tccaatttcc tcaactcctc caaactacgt
     3361 aaatgatata gtctcataat aaaataaaac ctctcagcta atgttgttga aattatccac
     3421 taaacctaat tgatattgct taggagatac acctgcgtac tttttgaata ctctcgaaaa
     3481 gtgtgaagaa tctgaaaatc ctaaacctaa agaaacttca ctgatagtat ggtttgattt
     3541 caacattatt ttagccgtag aaatttttcg cttagagata taattataaa tcgtaatatg
     3601 catttcattc ttaaagcttt ctcttaattt agtttcactt acagaaaaat aattagctat
     3661 ttcctttaca gtaattcttt tatagatatt taagccgatg aattgaacga cagaagaaat
     3721 taagggagaa agatgctcta tcttagtttt tccgatttcc ttagtataat aaacaatccc
     3781 agaatccctc acttttaaga cctcaggtaa cgatacagct aattcgtttt cttgtatcaa
     3841 agcatctcta ctttgatacg cttctattat atccattcct aatgttatag acgtttgtgc
     3901 taacttttca aaaacaatga tagaataatt tttttcagac cgaatcgtat ctccactaac
     3961 acttggaatt attccattac tcagattaaa aaccatgttt tctaaatttc gaatatctcc
     4021 atttttaaca tattccagta ttttattttc ataaagaaac agatatattt ctggatcgta
     4081 attttgtgat aacagattat ctattttgat ttgctcgata gttttactta gatttcctgt
     4141 atattcaatt aacggttcat ggaatagatc ttctattttt ccagtaaaaa aataatgaac
     4201 taaaatcaga atatctcgaa tgtctcctaa agaatacaaa gtcaaatttt gcaatgaatt
     4261 atagagtcgt tctctatcag ccattgcaac attcaaattg ttcattttta gtttaaaaaa
     4321 atctttatca atattatttg ctctaaatgg accaaataag taatatctgg ttttaaacat
     4381 gtatagaaga aacatctcat taaaatcacc agtaaagaaa tggaaatctt tagttttttt
     4441 aatattatca gttagtaact ttttataatc ataaaaaaaa taagtagttc tatctgagcg
     4501 atactctttt ataatactga attcggaatt aaaaaccacg ataggtaact ttaaacaaac
     4561 atgaagagac tttaataaga cttctttatc tgaaaacact tttgtttctc tctttccttt
     4621 ctacatcatt aaatagtatt ttatacgtta tctaaaatac tattcctctg tcaaaataag
     4681 ctaacttact tcattataaa atatccactt atcgctttca tgtatattcc accgtaaaaa
     4741 gtacatttta tcaccgagaa ctcatcattt ttcatcatga tgaatactat ttacatatat
     4801 ctagttcaaa gacaataatt attatgatcc gttattatct aagcactata cagataatag
     4861 gaatttttcg tttctaaata ctccaatttg ggaatccaat aaaaattttc gttcaaatac
     4921 gtgtctattc gaactctttt ctagttgtta attatgaagg gggaagaaat aaaaaataca
     4981 atctatacac gaataagaat attatccaaa aggaggtttt agaaaagatt tattatacgt
     5041 aaaaagctca agacattttc ttagccataa ggacatgttt gatatttaag aagatatatg
     5101 aattttcttt tctaagtata aagataacta tccattatca actaaaaaaa catttttgaa
     5161 aataaacatc agcagtccta tcaatgcatg tgagcttata gaagttattc gcagcgcagc
     5221 ataattgcat tagcttcttt tcgtgttttt tgcctctttt ccctttaaat cttaatggag
     5281 gaggtgtgca atatgtcaga taacgacgaa cgcgtaaatg cccagttgat tcgattcttt
     5341 gaaaaggtca ttttgaacac cgctaatact tattataaaa aacaagaaga aattcaaaaa
     5401 catgaacaac ttgatcaact cccaccatat ttgtttacta aagatactat aaaatttaaa
     5461 caaccgatta atgttttaaa tgtgactctc cttgttgaag atataaaatt agcaaaaatc
     5521 cttcctttgt tgaaagaacc tgaacaaatt tttcttatcg aaaaatttat ctttaataaa
     5581 actgataaag aaattggaaa ggttctgggt attacaagac agggtgcgac aaatttaaaa
     5641 catcgacttt acaaaaaaat tcaaaaaatg ctggtttaat atctgcataa taactaattt
     5701 tgtatgcttc tttgctcgtc ctcctccttc cccaaaaaat aaatagatta taagttcatg
     5761 ggaagaaggt aaatttatga atactttaaa agaagatttc attttagcaa agaatggaaa
     5821 tgaagaagca gttgtggcca ttctcaaaag atttagcgct ttggtccata agcaatcctg
     5881 gagaactgga aaatatgatc aagattgtta tcaagaatgt atgcttgcaa tatatctagc
     5941 tatctcgaaa ttcgaaatta aagaataact caactaaggg agtctgtgac ataagtaaaa
     6001 agatagtttc aaatttcgaa caataaattt gaagccatct tctttataat tataaagtat
     6061 cgattttaca gcgttctata atgattcata gaatctaaaa cgaatgattt taattattaa
     6121 cgttttttaa ttcgttttta gcttaaaaat aacttttgtc acagtctcac tcctaaaatt
     6181 actttattta cgtacgaagt gggcaggagg tcgaatccag aactgggcca tggtacttca
     6241 acgaggactt ctcctctaga attgaaaagc atgcgattta ccttccgtaa cgacggtaga
     6301 tttattcaac tatgtgtcca atcaaaattt gtctgatttc aaaaatagtc aagagaattt
     6361 tggttgttaa aaactcttat ctatcaactt ccacagttta gttgacaaac cctgatacta
     6421 agttgggcta tgatacactt ttcttgacta tcagttacgt taccggaagg aatgaactct
     6481 attgaaaaaa acatatgatc gtaaatttaa actaaagatt tcacaggata tccttgagaa
     6541 aaaagtaact accaagaaaa ttgctgaaga atacaatatc tctcgtccaa caatttcccg
     6601 atgggtttca gaatatcgtc gatacgggaa aaatgctttt gctggacaag gaaaaagatt
     6661 acctgataaa gcagatttct acattcttga acaggaaaat aaacgactaa aagaggaaga
     6721 cgagtgttct acgaacacaa agatcgatac ggatctccag ggatcactca aaaactttac
     6781 gatgaaggaa tcgaaatcaa taaacgtgtt gtagctgttc ttatgcgtga aatgaattta
     6841 tgcacaagag gatttcatcg acgcaaatct tctcatggta gagaaaaaac aatcaaagaa
     6901 atggtaaaaa aaatgttgaa tcgataattt gaacagaacc aaattgatgc tgtttgggtg
     6961 acggatatta cttacattcc atgtaatgac ggtcgcctct atttgtccac ctatattgac
     7021 ttagcaactc gtattctacg ttactatatg gtggattcac acatgaaaaa agagattgtc
     7081 gtccgtccat tacaaattta ttaaggagaa ttgcctacgg taattcattc cgatagaggt
     7141 agccagcatc ggtcttttat ttatcaagag ctgttgaccg accaccacat cacacattca
     7201 atgagccaaa ctgggactcc tgttgacaat gcagtaattg aagcgtttca tcgatcaatc
     7261 aaatgagaat taatcgactc caacaaatat aaaagtaaaa tagagatgaa ggtgttaatc
     7321 caagattatt tagaagacta ctacccaaac aaaagaatcc atacaaaatt tatgatgact
     7381 ccttgacaat ttgaagcgaa gaaaataagg gatctataat atctagtgtt ggtgacgcct
     7441 tctgttgcca atactttttc ttttttctaa taaacgcaaa cagacacctt gaaatccttt
     7501 agaataaagt cgacgaaaac cattcaaagg agtgtttcaa gatgtcctat tcagagttta
     7561 ccaaagagtt actagatatt ctagacctaa atcttacctt tcatgaagat gcctttcgca
     7621 aagagcgcat taatgacgaa acatgttttg tttttgatgg cacattgacc tatcaaccag
     7681 aggaatgctt ccattgccac tatcaaaata agcaaacgat cattaaatgg ggctggaaaa
     7741 aagtatccat cttattgaat gatgtcagta actacaagac cattgtgcgg attaacaaac
     7801 agcgcttcaa atgtaaacac tgtggaaaga cttttttagc agaagattca gttagcgatc
     7861 gtcgctgctc cattgcccgc agagtcaaac aagccattct tgagctatta agtgagccta
     7921 tttctatgtc tttgattgca cgtatgaagc acatttctcc gaccactgtc attcgaatac
     7981 ttcgcagcct tcggcctaaa accgtttctt tgaaccagcc gttaccagaa gtggtgtgtt
     8041 tcgatgaatt caaatcggtg aaaaacgtct cgggagccat gagctttgtc atgatggacg
     8101 gcaagaccca tcaactgatt gatattgtgg aaaatcggca actcaatcct ttacgcgact
     8161 atttcttgcg ctaccctaga aaagtccgtg aacaagtacg tctagtggtt tcagatttct
     8221 atacacccta tcgcacattg gtgaaagagt gttttccgaa tgcccgaatc gtcgccgatc
     8281 gctttcatat tagccaacac atcggtcggg cctttaccaa ccaccgaatt caagtaatga
     8341 agacctttaa aaaaggggat cgtcggtcga agcacttaaa gaaatactgg agactacttc
     8401 aaaagaatgc gtgggaattg aatggtcagc atcgctactg gcgtccgtca tttcgagatc
     8461 atctgacaga agcggagata gtcgatcggc tattatatta tgatgactcc ttaaagcgag
     8521 gctacgaagt ctaccaggtt tttctttccg ctatcagaag acaagatgtt cctgaattcg
     8581 tcgcgctcct aaaagaagac tacaaggagt tgccagagca ctatcaacca gtgttcacga
     8641 ccttcaagaa atatcgaaca gaaatcaaac gagcgttgcg agttccctat tccaacggcc
     8701 ctatagaatg tttgaacaac catatcaaag tactaaaacg aattgcctat ggctttagaa
     8761 actttcaaaa ctaccgagaa cgaatctttt tatatcgagg aaaatatttc aagaaaacta
     8821 agaacactac ccaactgact aaagccagaa caacaacacg gctagacaag atagctgtct
     8881 agccgtgtga gtatctcgat tattctattg aatttcttca ccaacactca ttgacgaaga
     8941 gccaaaataa gtaatctaac cgactgttaa ctaaaccatt gcaggtcccg ttaaagatac
     9001 ttgtgcctgt tgtcaccgct tctttttctt gtccacaagc gcttaacaca cttaataaag
     9061 cccctgctat tattaataat aatatctttt tcttattaaa aacctcctca tctatttcca
     9121 gattagtaaa tgtagtaatc tttgctttct tttttctcaa tactaataac taacttatgg
     9181 ggtaaacaaa tactcgtttg accgggtttt gagatccatc cagttctaac cgcgatttga
     9241 tcaggactat tatcttcttt aactcgaatt cttccatctt taatctcaac aatattatat
     9301 tgtcccttcg caggatgata attcacgagt ttatgattga tcttatccaa atcgaagcga
     9361 tccatttcct tgccatcaat ttgaacgatg gcatatttga tagcatcttc agttgtttca
     9421 ttctcctttt ctatagagaa tacaaataag ggaataaaag agggaataat caaaatgcca
     9481 ataattatga catcgcctat cttgacacca gcttttctta aaaatttcat taacgatatc
     9541 tggctaaaca tattagaatt tttccatgga aatggtgaaa tttttcggaa attatctgca
     9601 ttaagagaat cagttaaaca accctcaagt tgttccttaa tacattgttg atccatcttc
     9661 ataccaataa aaactagttt attaatgcgt tcaccaaaca acggatgcca ggcttccttc
     9721 aattcgggtt tttttgtata atcatttgac actcaatttt aggtaaacta gctacccaat
     9781 aatttaatga ttcaatactt ttatggctac ccgatgtttc aaagatcgtc gaaaaatcac
     9841 tatgttgggc aaaccaaaga aacccttttg ctcttgtaat attgccagga aaatttttat
     9901 ccgtaagatg ggcaaatttt tgtgcgtcaa ggagtaaccg atttcgatag ataaaacttg
     9961 agataccata ttcttctgtt tcaggaatat gattttgata gccaagctct aactctttga
    10021 accagcccat tgaataggaa gtctcatcta tattaaaaag atttgtattt agaattttcg
    10081 ataagtctac ttcaccttta attgtcctaa ttattttggc attggactgt aacttagcaa
    10141 ttagaccgtt aattttgtaa atcgtcttgg ttgacactaa gtcactatta tttaaaagta
    10201 atatgttaca acactcgaat tgatcaatta acaagccacg aatatcctgt tcttcttcaa
    10261 ctacattacc ttcagctttt ctttcctgaa tcttttcacc cgtttcgaaa ttatcccaaa
    10321 aacgttttac atccactact ataataatgg caccaatctc taatcgttgg gtcaaatcaa
    10381 tgtctaaagg gtttttagct aatataattg cttgaataat tggaattggt ttgctgattc
    10441 ctgaagcttc aataacaatt tgattcaagt cagaaaattg cactaattga tctagttcaa
    10501 caatcaaatc ttcgcgtaat gtacaacaaa tgcatccatt cggcatcgaa acaagttttt
    10561 ctccagttct tttaaaacct ttcttgtcga ctaatacccc atcaacattt acttccccta
    10621 tgtcatttac aatgacagca atttcctttc cgtgatcacc gtacagaata tgatttagaa
    10681 gtatcgtttt tcctgaacca agatagccag ttaagattgt aataggtatt ttttcatagg
    10741 gaacctcttt ctataccttg aatttacact ttaagtgcaa cacataaaaa tgagtcataa
    10801 tcgatatcca actagtataa gtttgtcgag aacactccta gaaggaaaat aattatgagc
    10861 cagtattgtc atcttactat actcaaacga gaaagaatct ttctgcttca cgaagaagga
    10921 ttttctatta gtataattgg caacgacatt cagcatagcc cgtttactat tttacatgaa
    10981 ctgagaagaa acaagaaaaa aactaatcac cttccaaagc ggaaaaaaat tatgcttatc
    11041 gaagaaaaaa atgcggacgt tatcacattc taaatgatcc gtgagtttgg tcaattgttc
    11101 gtcatttatt tgtcgatttt cagtggtcgc cagagaaaat ctcgaatcgt ttgaagcatg
    11161 aacatatgga cttttcaatt agttttatca cgatctaccg tgcaatttgc aatgatcttt
    11221 tcgatacaga acccctctca cacggcaata aaggcctgat ccgttcattg caccatcgag
    11281 gaaaaacaag acatacaaaa aatcatgtcg agtctcgtgg gaagatttgc cttactcatt
    11341 cgatccatgg ccatccaaaa tccgcagatg atagaagtga ttttggtcat tggaaagccg
    11401 atattgtagc tggcaaaact agtggtactt gtttagtgac tttgacagac agaaaatcat
    11461 gcttcctttt agcaggaaag aaggtcccca gaaagaaagc cgcttatgtg agcgagaaaa
    11521 tgattgaact tttaagcagt attccaaatg aggcggtcaa aacgatcact ccagatagag
    11581 gaaaagaatt ctccaatcat ttcgcagtta cagaagtatt gaataatatg cccttttatt
    11641 ttccagatcc tcatgccctt ggcaacaatg aacaaattaa aatacgaata gactcattcg
    11701 agaatacttg ccaaaatcga aagagatagc tacggtagat aactcaacga taaatcagat
    11761 gattgaaaaa ctgaataaca gacctagaaa gtgtttagat tggagaacac cttatgaagt
    11821 cttctttgat aaagtgttgc gcttgatttg acaattcaag aaataataaa aagcagatca
    11881 aatccaaata agggaaaaat gatctactct ggtattatca attaattggc caagctatta
    11941 tgccgttttg ctaaacgtct acttcttcca gtattgacct tgataaaaca acaatcagga
    12001 tcccgtttta atactctatg aactgtgtca atactaacat gtaaagttgc agcaatatca
    12061 gctaatgtac tcccatttaa tctcatttgc tttgcaaatt gataacgccg ataagcatca
    12121 atattcttgg aatccataga caataatact tttcttacca tgtatttcct ccaaagattg
    12181 gattttaatg ggccatcatt tcttgagcaa tctcatgctc agataatgaa gctggataga
    12241 aagtaggcca attatgcatt tcattataaa gtttttcttg actttctcca ccaaagaaaa
    12301 tatggaaatg ctctgcctta cttggtgcaa ttccatgatc gctaaattga atatatttaa
    12361 aacgaccagc acctgcatca tcagtttcaa aattaaaacg aacaccacgg tttccttttt
    12421 cataattcaa aattttgtat ccaacatatt tgtatgtgta ttgatgatgt tcaccgttga
    12481 ctacaaaatc aatggtgttg tctttaatgt tgatagtatc aatatctgtc ttatagccag
    12541 tagtataata atctttgtac tcggcagctg tcatgtcttt attgagtttt gctttgtaat
    12601 cgaaaacttc atccaaaatt ccatctttta acaacggata aacagattgc cattcgcctg
    12661 tataatcact caattcgcga tccttcacat cggcatcttt aaagtaccca ttagcaactg
    12721 tcttttcatc cttcgcttct ttttctggaa ctacctcttc accagcaacc atagttgttt
    12781 tctctaacgc ttttagattg tcttccatta ctgaaacata atcttcacca ttatccattt
    12841 gttcttttgt aagactttca agaggattca atacttccaa ttttatccca gcttcattgg
    12901 ccaaagtttt tgcgatttta tcattagcat tcttttcaaa gtaaatataa ttgatcttat
    12961 tttttttaac atactctttt aattcagcta atcgaccaga agatggctct tcttcaggac
    13021 ttagtccagc aataggaact tgtatcaatc cataatccaa agctaaatat ccaaaggctg
    13081 catgttgtgt cacaaaactt ttttgtttcg cttctttaag actagtggta tagtcagcat
    13141 ccaaccgttt cagtttagtc aaatactttt cagcatttgt ctcaaatact tttgcttttt
    13201 tcggatacaa tttaactaac tgatccttga tatttgaaac ttccttaatt gccattttgg
    13261 gtgaaaccca cgtatgagga tctagttcat gatggtgatc ttcttctccg tgatcatgat
    13321 cttcttcttc actacctggc aataaaatca tgtcttttgt tccttctact acattaggcc
    13381 cagctttctt ccaactttct ttagcttttg gtacccatga ttccatgttt tcgttatgat
    13441 atacaaaaac atcagcatca tgagcttttg ccatatcctt agctgagggc tcatagtcat
    13501 gaggttctga tcccgctgga accattaaat ccacgttcgc ttcatcgccg acgatgtttt
    13561 tggtgaaatc atacattgga tagaatgttg ttacaatttc taattttcca tctttatcat
    13621 tcgatacttt tgcatcatta gtgctggagc aagctcccaa taatcctaga gctgctatcc
    13681 caataagtcc tacactgatt ttcttcattc tttttccttc cttaattatg ctaatttctt
    13741 cattttattt tgtttatgtt ctttgataat ttttaatgcc ctttttctag ttttgatatt
    13801 cgggctttta agtcgtcgat atgcactaga tatatccgtt ttctccatta gaaattaccc
    13861 ctaatcaata cttatcatta tttgctttcc gccagtaatt tatcaaaact ctgtttgatc
    13921 ctttcttcat ccaacatttt tccaattaga ataatagtgg tttggtcttt ttgtcgagtg
    13981 gtgtattgaa aattaacctg ttcgtttacc ccttgtatgg caactataaa atcttgatct
    14041 tgcaaagcaa tcaatccttt aaaacgatac aggctccctt ggttggttaa aatcagccgg
    14101 tcaattaatt ggaataaaaa atcttttttt atgtcttgat ctgaagttaa gagaattgat
    14161 ttaaactcat gttcttgatg atgttgatga tagtgtttgc cattatagtg attttctgat
    14221 gaatcacttt caaaaactaa gggatgattg aatttttcca aattataaaa atcctttttt
    14281 tgaatcgttt cactatgtgt gaacattcga atatcggata aagggttaat acttttgatt
    14341 tctctctgta atttttttag tgcagataaa ttcaagtttt gttttctaga cacaaaaatg
    14401 cgatctgcta tgacaagttg cttaagggct tgtggttctc tttcaataag ttcaaaactg
    14461 tcactatcaa taacagtcaa taaactatcg atatatactc ttcctcttag ttgaggcgct
    14521 gttaaaagtg tttgaatgat tggttgggga tctgctatac ctgtagtttc aatgatgatt
    14581 tgtgaaattg gttgttgatg ttcttctatt gtttccagta aagaaatcaa tattgctaat
    14641 aaatcactgc gaagactgca acacatacat ccattattta gttgtaacac ccgttcctcg
    14701 ctatgaacta ataatttgtg atcaatccca acttcaccaa actcattttc aattataata
    14761 atttcttccc tggggtaggg tgagacttgc aaggcttgat tgattagcgt tgtttttcca
    14821 gcccctaaaa acccagaaac gattgtaact ggtatgccca ttttgtcacc tttttaccac
    14881 ttagctattt cacttctttg aaaattgccc gtctgcgcaa ttttggcgaa tactttttta
    14941 attctaaccg ttcagggtta ttccgtttat ttttgctggt gagatacaga cgttctccag
    15001 tttcgacaca ttccaaaata atattttgac gcataaatct tcctccttca atacttatcg
    15061 atcttcaaga tatgcttgaa tttgttgtgc atcttcaaaa gttaactcgc cagattccaa
    15121 ttgtgaaact aaattttctt tcatatcaac aggtatctcc caataaccat caatacattg
    15181 aaataactcc tcgtcatact cttcaaaaat tggatagatt cttaaaaaat catcaaatga
    15241 taagccttgg tcctcatagt aatctttgac taatccattt tcaattgcct gttgggtcgg
    15301 taaacccgtt tcagtaataa ttcctctctc taccatcgaa cgataaaaag tgcatttatc
    15361 tagcgattgc tgggtcgttt caagataata ctttaatcct tcttcaacta aaggtgggta
    15421 aataatttcc attttgattc cctcctaaaa gttatatatt aaactatttt gaagctcctt
    15481 taattacttt ctttccatta tagtaccctt ttaaagatac atgatggcta cgtctataat
    15541 caccattcgc ttcgtcaaat gaaatttctg gttttactac agtttgatga gttcttctta
    15601 accgtttctt cgccttagat gtttttcttg ctggtactgc catgatattt cctcctttta
    15661 ccaacttgct ttttttactc caggaatcaa cccttgatgg gcaagttctc taaacttaat
    15721 acgggacata ccgaactttc gcatataccc tcttggacga ccatccgtct gatcacgaag
    15781 cctcagacga ttcggatttg aatctcttgg caatttagcg agagcggtgc gatctcctgc
    15841 ggcttttaat tcctgacgtt tatctgcgta cttttcaatc atcgccattt gtttctttgc
    15901 tttagcaatt ttcgactttt ttgccatata cctttatact ccttttattt tgatttagac
    15961 atctcaaatc gtaatcgtta cgttttgaca ctgatacttt accaacaaga gttgctcttg
    16021 tcaacaataa ataacatgaa ataaaactca tatgaataga taactatttg aaaacgaatt
    16081 tatcggtaca tagagataat caaatcattt aaaagaatat gcagtttaag ctctgaaaaa
    16141 taagaattca cttttattag tccaatcaaa cacaaatacc gttttaagta gcttttcctc
    16201 tacttaaaac ggcaatatga aagaaactat aactttttat tcaatctttt atgtttaact
    16261 ggtttatcgc caatttttta aaactttgat tattccttat attataagaa tagacattaa
    16321 gtgaactaat tcaatcatac tgattctttc gtgtattcaa aattagatgc tgtttaataa
    16381 aattattctt tttgaaaaag aattataact atatcccaaa aattgtaggt taatttaaag
    16441 cctatttcat catcgtgatt gagcttcttt ttcatagacg aatttctcag atagtttagt
    16501 tttctttatg agatttttgt atatttctga ccttcgatac aactcactat gagtaccttg
    16561 atcaactatt tttccttgat ccatcactac aattttattt accttttcaa tagacttcaa
    16621 gcgatgcgat ataattagta ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat
    16681 acgtttttca ttttccgcat ctaaactggc agtaatttca tctaaaatta aaattggcgc
    16741 atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc tctcctccag aaagtgaact
    16801 tccgttttct ccgaccaatg tatgataacc gtcaggtagt ttttcaataa agtcttcgca
    16861 gttagctaaa cgagctgctt ttttcacttc ctcatctgac gcttgactat ttccaatacg
    16921 tatattttcc aaaatacttg tattaaacaa agttacctct tgaaaaacaa tagcgatttt
    16981 gctaaacaaa gaagctggag atacacgttt aatatcatac ccatcaatta aaatacagcc
    17041 ttcatcataa tcatacagtc tagacactaa tttcaagata ctagttttcc cggaaccact
    17101 agcacctact aacgcagtca cttctccttg tttagccgta aaagagatat gatctaaaat
    17161 tggggtattg ttatcatatg agaaagagac atctcgcaac tcaatgtcaa aagatttcag
    17221 aggactatct gatccctctt ggatactggt ttccttcata gctctaattc gctgtatttt
    17281 aggagctaaa taaaatattt caagcactgc ttctttcata gaatcgaagg aatcctttat
    17341 ttttattgcg gctagtaagt aaccaacgac gtagagtata gtcacttcac ccgttaacag
    17401 taagtgaaca cctactagaa tgactactgc taaactaaca tatgagaaaa tagaggataa
    17461 ggccatgact gaaaaagtac ttaattctac ctttaaatga atcctttcac tctcttccat
    17521 ttttttgaaa agtctgtctt gaactttttt agatagatta aagctattaa tctcttgctg
    17581 caattctata gtttcttgaa attcttccga gttttctctc aaagtatcgt aatatttagt
    17641 attggctttc gtttgggatt tctttgataa caagattaag acaaaactaa ataacgttgg
    17701 caaaataaca gctaatccca ttttgacatt accaaccaaa agcatcactg aaataaaagg
    17761 gaaaaacaga gccataccgc cggctttagg tattgcatga ctcatcgcat gctcaatacc
    17821 ttcaacgtca gacatgatag tttgagataa atctgataaa ttatgttttg aaaaatagga
    17881 taatggtaaa tttgacaaat cgtctgcaat ttgcattctt aaatgcgcac tttctttata
    17941 agtactatta tataagtttt catactccct atctaacaac caatacatga aaagcaaagt
    18001 aacgaacgat actgtaaaat aaagccaagg cggctttaca ttttctaaaa ctaattcgtc
    18061 tatcagcatc attataatga atgctggtac catattaatg cagtatacaa aaaagctaga
    18121 aatagatgct ttaaccaaat cttctgcgcc ctgattagtt aaagcgaatt tgtttttata
    18181 aaaacttttc attgtcaagc ctccaatcat ttgcattctc ataattagtg accaattgtt
    18241 tatacacttg atgtttagcc attaattcat tatgatttcc acgttctact attttccctt
    18301 ctgacaagac aagaatttca tctacagatt taatgctgga taatcgatga gctatcataa
    18361 taactgtttt ttctttcatc agatgttgaa aagccttttg tagttcatac tcattatctg
    18421 gatcaataga tgcactggct tcatccataa taacaatctt tgcatctttt aaaattgctc
    18481 tggcaatagc tatacgttgt ttttctcctc ctgacagata aactccttta ctaccaatta
    18541 aggtatgctc tctttcggga aatttttcta aaatcgattc gcaaccggct aatcttagcg
    18601 cgtcaaatac tttacttgct ggcgcttgag gatttgctat agaaacgtta tcgtaaatac
    18661 tctgattgaa tagtttcgta tcttgaaaga caaaagcaat tgtgttaata attgtgtctt
    18721 ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg tcccttatcc acattgtaga
    18781 aacctgaaat tagtttagct atcgtactct ttccgcttcc tgattttcct actaatgcat
    18841 aaattctttt ttgttctaaa actaagttga aattttctaa aacacactct tctttgtatg
    18901 aaaagctgac attttcaaat gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt
    18961 ttagttggtc ttgcttcatt tctaaataaa gggagtctag tgtttcgact gcatatcttg
    19021 cttcaaacaa gtgcatactt acatacatta ttctcatgaa agaaacaaaa ataattccac
    19081 ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt gggagtggta agagaagtta
    19141 taaaaaaggt tataggaact atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa
    19201 gaacaaaagg tgttttccca ctcaaagaat acttatatgc aaacttagca taatcgttaa
    19261 ttgctttatt caaggcttta aatgatgtta ctttagcacc aaatatcttt ataacttgaa
    19321 ttcctcggat atattctacc gtttcagagc ttaacacatc taacgattct tgatattttt
    19381 gaataaaatt tgtattctct gtcattttct ttaacaagaa tacaccaatt actaacatgg
    19441 cgattagaac caatccaatt ttaaaattaa tgaaaaagcc taaggcaact gataaaacag
    19501 gtgttactat ggcttgagca ctatctggaa tcaagtgagc aattgccata tgtgttttag
    19561 ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga atttaaatca aaaaaacgaa
    19621 aacttgcttc tgttaaaccg ctaattccct tttttctcaa attagtctct aagcgaaacc
    19681 caaggatatg agaaaataag ccagaaaaaa tataaaataa agctcctaaa gttaaccaag
    19741 caacagtttg aaccgcataa ctcttagcta aatattcatc tcctacaata attagagact
    19801 taagaaattt gtaaatactg taatatccat agactgttaa tacgacagaa aaatcagaaa
    19861 ataaagtggc taaccatccg tagtatttca ttttagggac ataactaaat aattttttgt
    19921 atactttcat tttttcctcc cccgcattca atttcttctc ttattcgttt acaactgtta
    19981 atgctttccg taatagcgta aattagagat gtacaaacag atagaaggta aactacgttc
    20041 tattttttgt atttttttaa gacttttctt ccaatcaata caccaatcat agataaactt
    20101 gctacaacta acactgaaat gattccccat atacctaccg catagtggat cattgcatcc
    20161 attgttgcag cagattgtcc tttatctatc caatcttgac gaaaacgatc tgtaaagaac
    20221 catataggaa tccactgtcc ggcagcactg cctaattgca ttagagccca agacgcaact
    20281 tgaggaattg ctcgatcata tccaaataca gatagaataa tatcagctaa gatcgccata
    20341 ctaatcgaac tgataaacca aggtaagtac gccccgccca ttaacgtaaa aaataacaat
    20401 aaaacaattg tgtaaatagt aaaaattcct tttctagggt agttatagct aatataaaca
    20461 aatatgattc cagataataa tccaaaaatc cctggggata tgctgtgacc aaacgcccca
    20521 ccaatactac taaccattat tgaaaaaaag taaattacta gggaaataac tgtagctaaa
    20581 ccaataaaaa tataatcttt tgttttcata tatattctcc tcaattctat ttcgttatgt
    20641 cattaaaaat ctgttctatc aaagttgaat cttctactct aagttttata tcttttttta
    20701 ttccaccatt ttttaaataa ataattctat tgcatgtttt ataaagaaat tccggatcat
    20761 gagtggctat aactactgca cccttttctt ttaagtcttt aataaggtca gcaatccttt
    20821 ccatattaat ataatccaac ccacttgtag gttcatcaaa tagaaataaa ttagtttttg
    20881 acaatgaagc tatagaaacc aataatcttt gtttttctcc accggataaa tcaaacggta
    20941 attgatcttt cattttataa agttccattt tttttaaaat tttttcttgc tgatctttac
    21001 taactatttc attccccagg gctaactcgc tcctcactga ttctgtgaaa aattggtaat
    21061 ccatttcttg cattactaaa aacggcgatg acaaattttt tttattttcg attatctttc
    21121 ctttggtagg gcgaataatc ttcatgagta ttcttagtaa ggttgttttc cccacaccgt
    21181 ttttccctac caaaccaata acatctccaa ctttgacatc aaaatttatg tcttttagaa
    21241 tattttcata agtaatttct tcaactttta atacatctgc acttaccttt tcgatatagg
    21301 gaacttcaat cgaactatta aaaatgtcaa atttccttaa tgcgttattg ttttcagttt
    21361 ttaacaactg aatagttgta gtttttacag ttctattttt aacaaataga acttgatcaa
    21421 ttaattcttt caaatagtaa aaacgatgtt cagctataac aatagtaaat cctttatttt
    21481 ttaaatcaca aagtaagtca gcaagtttct tagtcgcttt gaagtccaaa tttgaagacg
    21541 gttcatccaa tataattaat ttagagttta gcgataaact acatccaata gaaattttct
    21601 gccgttcacc acttgatagc gaaaaaatgt ttttatctaa tatatttgaa agatctaata
    21661 attcagctaa ttcattgatt cttttatcca tatgctcttt tgtaaatcca aagttttcca
    21721 tcgaaaatac taactcactc gttgtatttg ttgtaaagaa ttgtcctcta ggatcttgac
    21781 ttacgtagcc aatatttttt gatatacttt cttgtgttaa ggattgtagc gagttatttt
    21841 caaataaaat ttccccaacc aattttccgt cataaagtct tggaataagt ccattcatta
    21901 actttaatag tgtggatttc ccactcccag aagaaccagt aatcaatgta agactacctt
    21961 tttttattga aaaattaagg tcaacaagcg cttgttcctt aacatcctta tatgaaaaag
    22021 aaacattgtt aaattcaaac aattctttca cctcaaatca aaatacttaa gaaaaaaata
    22081 gtaacggaaa acaggatgat atagtctaat aagctcatgt ttgttttata gtaagaactt
    22141 cttttcacag gagattctat cccctttgtt attgcattag cggtcatttc ttcagcaatc
    22201 atagttgcac gtataagaac gggaactaac agaaattcca aatattgcag aggctgaaaa
    22261 aaagacaagc gaatgtttct catttttaaa gattctttaa tcattcttat ttcagaacga
    22321 taagtcggaa agaatctgat cactactgta atacttaaca ttattttttt cggtaaatgt
    22381 attttttcta aagaggataa taattcattg ctagtataga cactaaccaa caaccatgag
    22441 atcattacga ccggcaacat tcgaaataca atatataagg atgtcccaaa aaatatagta
    22501 aaaaactcta tcttgatagc atcaataatt ttcattacta aaaacagaac tgaataaatc
    22561 aagatgaaat aaatacatgt ctttatttta cgaaacatca atactaaaac tgtagcaata
    22621 atgaaacacc ccacttgaaa tatatttccc tttcctaaaa aaataatgac cggaaaaaaa
    22681 atatttaaag taatcaaggt taacggattg atttttgaca ctctttcacc tccaaaaaat
    22741 aagatcgcac tgttatctta agctgataaa aatgcaccgt tattcagata acagtgcgat
    22801 cttatcaaaa agcataactg agaacaattc tcaatgcaag ctttttttta attaactcag
    22861 taattcaatc cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt
    22921 ttcttttgaa tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta
    22981 gaagtggatt tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa
    23041 cttatctaga caacagactg tcttttttgt cacaaatgat gtaatttttt caacaatatc
    23101 ttgatatttg gttccagctg attggaataa aaccaaatcg aatatacgtt tattctcaaa
    23161 aatataatta ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc
    23221 tatattttct aagtttaaga ggtatttggt aaactcttca ttatacatat taataagcgc
    23281 attaatatgc ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa aatgtttata
    23341 aaaagcaccc gtagttacgc ctgcggtcaa gcacagttgc cttatcttag tttgctgaaa
    23401 tcctactttt tcaaaatggc ttagtccact ttctattaat ttttggtgag ttgtaatata
    23461 atctctggtt tctatgggta cttcctccta ataagatgaa ttaactatat tatcttacac
    23521 aggacttgaa gtttcaatcg ttaacgtcta ttactgaact cattccttag agctaaacta
    23581 atgagaaaga gcaaaataac tgatacaaaa agcaatccta caagaattaa tagactcatc
    23641 taaatcattc accttttcta aaataatccg agaaagattt aaaaaaatta tatccagcta
    23701 ttaggataag tcctatcata atcactccaa tagccatttt cttatcctcc ctaaaaataa
    23761 agccttttac ttaaaacctt tttttgtaat cgtcaataaa ttttttccct ttatcagtta
    23821 attgaaatcc tatatctaaa tagtcctcat atctaagttt agtcagcatc tcacttacag
    23881 atgctggact aacacttaga acttttgcta aatcattatt ttttaatttc ttttttcttt
    23941 ttgataattc ctgaattgct agtaaataaa ttagtatagt agttgtttta atttgtacca
    24001 tacctaccct ccaaattatt tcgaaaggcc ttcagcaatt ttatccaggt tccatttcat
    24061 catcgcatag taactatcac catcttgtcc tttttctgca attgaatcag taaaaatcgt
    24121 tgaatagata ggaatattgg tatcttttga tactgttttc attggtctat cgtccacact
    24181 gctttctacg aataaggagg gaacttttgt tgtgcgtaat ttttcaacta agtgttttat
    24241 ttgatctggt gttccttctt cttcagtgtt gatttcccaa atgtaagcag aaggcacatt
    24301 atacgctttc gagaaatatt taaagcatcc ttcacttgta acaatcattt ttttatcatt
    24361 cggaattgaa gcaaatttag atttagcttc tttgtctaga gaatccaatt tttcaatata
    24421 cttatctaga ttttctttat agaatttttt attatcagga tctttttccg ctaactgttt
    24481 ttcaatattt ttagcgtaaa taataccgtt ttctaaattt aaccaagcat ggggatcttc
    24541 cttccctttc tcactctgac cctctaagta aataacatct atgccatcac ttgctgcaaa
    24601 atagtctttg ttttcctctt tgttcgcatt ttttactaat tttgtaaacc aagcatttcc
    24661 tccagtttcc aagttaacac cgttataaaa aatcaaatct gcttttgaag ttttttgaac
    24721 atcttcaggc aaaggttcat attcgtgggg atctttccca ataggtacga tactgtgtaa
    24781 atcaatttta tcttttgcta tattttcagt aatatctgct aaaatcgaat tagtaactac
    24841 tacctttaat ttttcgttcg aagccactgt atctttgtct ttactatttg ttgaacatgc
    24901 cccgaatata aaaagagcaa tcaaaatccc cagacttccc agtactgctt tccattttct
    24961 catcataact ttctctcctt ctttcttaaa aatttttgtt ttggtgagat tagaaaacta
    25021 attacaaata agaatgccgc tgttaaaaca atacttgatc ctgctgctat gttaaaacta
    25081 taaccaatga acaaacctag caatgatgag cacgctccga ctgtcgccga aataaatatc
    25141 atagttttta aactgtttgc atataaatat gctgtagcag ctggtgtaat taacattgca
    25201 acaattaata tagttcccac actttgcata gctgttactg ctactaaagt taatagaatc
    25261 attaatagat aatgataaaa actgacattc atccccattg cttttgccat taatggatca
    25321 aaagaagtga ttaataattc tttaaaaaat agaacaactg taaccaacac taaaacagaa
    25381 acgccaattg taatccactt atcaatatct tgtacagcta aaatatttcc aaataagata
    25441 tgaaataaat ccgttgagct atttgcaact cctataagga taactcctaa tgctaaaaaa
    25501 gaactgaaag taataccaat tgctgtatct ccttttatta cactattgct ttttataaaa
    25561 gtaataatga tagaagctaa tagaccaaat acaatcgcac caataaaaaa attaatacct
    25621 aaaatataag aaattgctac tcctggcaaa acagcatgag aaatagcatc acccataaga
    25681 gacattcctc ttaaaataat aaaacaacct actgttccag aaacaatccc tataactaca
    25741 gatgtaatta gagcattctg taaaaagtga aagtcataca gaccattaat aaaattttga
    25801 agcatattat tccccttgta tattagagtt agggttttaa ccttccggtt ttgattggat
    25861 aaccctatga accatcgtta ttttaatttg gtattaactc atttcttttg agataatata
    25921 tttattggat tgagaggcta agccgttccc ccctctccaa taaatatagt atctccatag
    25981 gcttttttta aattctcttc attaaataca gattctacac ttccgtgagc aattagattt
    26041 cgattcaaaa gaataatatt atcaaaatac ttctttacct tgcttaaatc atgatgcaca
    26101 attagcactg tttttccctg atgtttcaat tcttttaacg tatccataat tattcgttca
    26161 ctaactaagt ctatccctac aaatggttca tctaaaaata tgaaatccgc attttgagca
    26221 agacatcttg ctaaaagtac acgttgaaat tgtcctccag ataattctcc tatttgatga
    26281 ttagaatatt ttcccatatc gaccttttcc aaagcctttg aaactctttg ccattctgca
    26341 tttttaattc tttgaaaaac tttcattccg gcatatgttc ccatagatac acattctttt
    26401 acttttattg gaaaagtaaa atcaatatca gctttttgct ctacatatgc aatcttcacc
    26461 agttttttat tgatatcttc attattaatg gagacctctc cctgatgagg gataatattt
    26521 aacattgctt taagcaatgt agattttcca gcaccatttg gaccaataat acctgttaaa
    26581 gcaccttctt gtatttttaa agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca
    26641 ttaagctttt tcacttctaa cataactttc tccttctaaa tgataatgat tatcacgatc
    26701 atatggtata caaaaattat attttagtca accctaaatt ttataattta gatttttttc
    26761 tgttgttgaa gattaggatg tcaaaattat ttgaaactac tcgtatagta aatagtaagt
    26821 aattttcaaa acacgaagaa aaacaaaaag aaaaaaggaa acgccgaagc atttcctttc
    26881 agtgatttaa tgagaacaac acgatttgac ttagagtctc aaaaagtcga ataggtacac
    26941 tcatttaagt gtctccattc gactttttgg ggatgagctg gctattgagg tccgcttacc
    27001 ctattgaata aatacaagat tgggtatttc taataagtcc gcccttcata ttggggtgat
    27061 cggggcgaac atgccatctt ttcatatacc acctcctcga cactcaataa tcaaagattc
    27121 tcgttaatct tcttcgatgc ttgcaatgat gttttcaata ttcatataac gaaaatcttc
    27181 ttgtttgata taaacgatct gagcggtaca tttttccttt ttgatgattt cttttacttt
    27241 ttcctcagaa cgtgcgacct gcggcgttaa aagtaaaacg tcatagtctt tataattcag
    27301 tttttcttca acgtttcgga tcgttgcata atctatctca tacgatagat ttttggctct
    27361 gatcgtttca agcatttttg atacgagcag cgacgcactg attccggtct cgcaaataat
    27421 caatagatcc ttatgcataa taccctcctg tttttagcct gcaaagccaa ttggtgaaaa
    27481 tgccacataa gttaaaataa caatgactac caatgctata gcagtcacct tggtgtattt
    27541 ccaaggctcg atatcgacct ttgtatcgaa tgagttcaac acaaattctc catcaggttt
    27601 caatttggaa accaccatca aaatgataat gtccagaacg aataatgccg tcaaaacata
    27661 aaggaaatgg atattttgga aaagcatttt tgatagtgca tacaagacga tatgcacggc
    27721 aatcgtcgtt tttgccccaa aagcagttgc tttttttgaa taaaaggcaa agagaatgat
    27781 cacgagcagc ggcatgttgt atagtccatt gaattcttga acgaactgat acaatccagc
    27841 cggtgcaaaa gaaatgaatg gcgctacgat aatggagata atccccacaa cgaccgttac
    27901 tgttttacca gcacgagcga tttgcttatc atttgctttc ttattgataa gcggtttata
    27961 aaaatccagt gaaaacaagg tcgatgtcgc gttcaaagct cccgcaaatg atgataagat
    28021 cgcgccaaag ataacagcgg cgaaaatacc aaagatcatt ttaggcaata cttcactgac
    28081 taaaaaagga tacgcgttat ctggatttga cattaaacta tcgccaaaaa tattgcgagc
    28141 gacgatccct ggaaagacaa ggaataacgc acctaagatt ttgaagacgc cgactaggaa
    28201 agcgcccttt tgtgcctcag ctaaattctt gccggacaat gctttttgga cgatcatttg
    28261 attggtacac cagaaataca gattgttaaa cagcatccct gtaaatagtg tcggccatgg
    28321 aacaacagcc gagtcgatcg atcccacaga gttaagcagc catggcgtgt ttaccacaac
    28381 gtgttcgatc ccgccaacaa agctgccgtc accaagcttc attaagccta aaattgggac
    28441 cagcagacca caagccagca gaccgactcc ataaatggag tcactattgg cacttaacga
    28501 caagccgccg atcagcagat agaggatgcc gacaatacca attgccatcg ctacaattat
    28561 gatcgcgacg atcggctgga cgcccagcag ctcatcaata tggaagatct tattgaacac
    28621 cagcgatcca gaataaagaa ccacgggtaa gaaagaagtc atataggtca caataaataa
    28681 gatcgaaata attcgtttgg taaccgtatc ataacgaatc tcgatgaaat ctgagattgt
    28741 attgatgccg tatttaaaat attttggcaa gaatatcaca gccaaagcta cgatcgcaat
    28801 cgtagcagtt acttcccagg ccattacttc catccctgca tgataacttt gaccattttg
    28861 tccgactatt tgttcggtgg acaggttcgt catgatgatc gtgccggcga ttggcaaagc
    28921 agttaagctc cgccctccca tgaagaaccc ttctgatcca gaagtatcga cacttctgga
    28981 tctagaatag gcaaataacc atacacagat gacaatcact aggaaactga ttatagaaaa
    29041 aagattcatc gtctagctcc ttttcaatag attaaagacc ggtgacctct cgaatgtagc
    29101 ttctggcttt gactgcatat tcaaatggat tggctttagc cgggtcctgt tctgcttcaa
    29161 ctacgatcca gccttcatag tcgctggcat ggattgctgt ccagattgga gtgaaatcaa
    29221 tcatcccatc accaggaacc gtgaagacgc ctttcttcac gccgtttaag aagctgtctt
    29281 tttccttttc tacttcttca gccgtttctt gacgaatatc tttgaaatgg atgtgtttga
    29341 tacgatcttg atgacggttg taaaccgcga ttggattttc accagaaaag accaaatgtc
    29401 ccgtatcgaa caataattta actttgtctg gatcagtcat ttccattaaa cgatcgattt
    29461 cttccgtcgt ttgaacgccg gtacccatgt gatgatggta aacgatctcc atatcctttt
    29521 cgtgggccaa atcccctaat ttttctaatc cttccgccag ttttttccac tcttcatcag
    29581 tgaaaactgg tttatcatca aagactggcg ctgtcataat cccttggata ctatgaccct
    29641 gctcagctac tacgatgact ttagcgccca tcgcatgtaa gaaatcccga tgtgctttga
    29701 aggcttcggc agtttcttca aaaggtttag ttgtcagaaa cgcactgaac catgcactgg
    29761 caacctttaa gccgcgaata ttcaaatatt gttccaacac ctctggatct tttggatatt
    29821 tgttgccaat ctctgttccg gtgaacccag caagggccat ttcactgata cattgttcaa
    29881 aggtattttc actaccaagc tcgggcatat catcattggt ccacgcgatt ggtgcgatgg
    29941 ctaattgaat ttgtttggtc atattctcac actcctgctt atttttttag ttgtactaaa
    30001 ctcttcgtat cgaatgattc ttgacaagcc aacgcgatct tagttgattg caatccatct
    30061 tctgcattaa ccgctggttg acgtttttct tgaatacatg agacaaattc ttgggcttca
    30121 cgaatgaagg cttctctgaa tcgttctggg aagttttgtg aacatggacg gatgatccct
    30181 tgatcgttca tgatcgtcac aagatttttc tcaggcgctt gcgctacccg cagcattcct
    30241 ttggttccca tgatctctgt ttcgacatga tagccatggg ctgcgtttct gccggcaact
    30301 agtaaagcta ttgttttatc gctcaggctc atcattgcgg ctccagtttc aagctcacca
    30361 gcctgatcca attctggata cgccgcattt ttcccaatcg cccacacttg atcgacttct
    30421 tggccggtaa accagcgaac caaatcaata tcatggatcg ccatatctaa gaacaagccg
    30481 ccgctgtcgt tattcatggc aaatttgaca aagctttcca agccttcgct cggatcgatc
    30541 ccataacaac gaatcagtgt gatttcgccg atttcgccat cttctaccat tttcttcgca
    30601 tactgatacg actcatcata gcggcgcatg aagcctaaca tgaagacctg ttcaggatgc
    30661 tcttcgatga ctttttgggt ctttttaata tcttcaactt caaggcctaa tggtttttca
    30721 gtaaagacat gcttgccatt ttccatcgcc aagcggactt ggtcacagtg aaagcctgat
    30781 ggtgaaacaa taaagactgc atcgatcacg gaacttttga ccatttcttc atagctggaa
    30841 aaagtatgct tgacgcctaa tttcttctgt gcgaatgcta attctgtttc aactaaactg
    30901 cacgcagccg taagctcaca acaactgaca cctgttgcta aattttgtgc atgtactttt
    30961 cctaatcttc ctaaaccgac gatgcctact ctaatgttgt tcatgaaata cctccgtttt
    31021 ctgagaatta ctcattgtat agtgctggtc gttcatccat ttgaatcatt acttttgttt
    31081 ttgtttcacg agactcatga caagccatca tggtgatcgc tgctacatac ccatcccaag
    31141 cggtaggacc gacaaccgca tcatttttga tcgaatcgat ccacagcttg aattcataat
    31201 cataggcatc caaaaagcgg gtctgccaat ctggtgaaac aggtgtgtaa tttttttctt
    31261 ccgatttaat tcgtgtatag gctgggtccg tcattcctac ttctgccgtt tcgaaaacca
    31321 catcacattt aatatcgtag ccaaagcggc aattcacaaa gatttccaaa tcaatcgtta
    31381 caccggattt tgtttgaaag atcagcagct gcggatcatg caaatcggca tgggagttgc
    31441 tggttttttt cggcaagatc atttgaacag attcataatc ttcatttagc aaccagcgaa
    31501 gcacatcaat ctcatgaatc gcggtgttgc tgatcgccat cggtgttgtg tagttttcat
    31561 ctgcgctagg atttctatgg gcacaatgca tcattaatgc ttctccgtat tgcttcgatt
    31621 cgattgcttc tttcagttca atgtagccgc ggtcatagcg gcgcatgaag ccaacctgaa
    31681 ccaatttctt cccataagca acttccgcat caacgatccg gcggcagcct gctgcatcca
    31741 tcgctaatgg cttttcgcag aaaacatatt tcccatgctt gattgcttct aacacatact
    31801 gctcatgggt cgggtcccaa gaagtcacaa caacgacatc cacatcttca gaagcgatca
    31861 atgcttctcc ggtttcaaag aatttagcat cgacacgttt ggcaacctct ttagcagctt
    31921 ttacattgat atctgagact gctgtcacat aagccccttg tgttttctga ttgatcctgt
    31981 cgatatgctc gatgccgatt gcacctgttc cgataacgcc tacttttact ctcaattact
    32041 tatccccctg ttctgttgtt taagcacaca tagtatgcgc ttacaaaatt attcctgtgt
    32101 agcttttatt cttaaaaggt agagccctct ctttgctccg ccttttacta cccataagct
    32161 tgtacgtata attccttaat ttgactatga ctaggcacct ttttattatt ttgcggactt
    32221 ccgctggtca acgcatccgc ggccattttg tctaaggctg ccataaacgc ttgctgatca
    32281 ataccataat ctcgaatcgt tgggatcgac agctgctcac agagctcttg gattttttta
    32341 atcagataat ccgcagcctc ttccagcgat aaatcttcgg ctgccggata aataaatctg
    32401 gcgatcgtcg ctaactcagt ctgaatagca tcctttgaga attcaaaaaa gaccggcaat
    32461 aacatcgcat ttgatatccc atgaggaatg tgaaaaagtg cccccaccgg tcgactcatt
    32521 ccatgaatca atgtgactga cgcattcgaa aaggcaatcc ccgcctccgt tgaagctaag
    32581 ctcatttgtt ctctggcttc agtgtcgtcg ctattttcat atgcctgaga aagcgtgttc
    32641 aaaattttct tcatcgcttc cagtgaaaat agttgcgtta cttcattggc tttgactgaa
    32701 acataagatt ccaaagcgtg acatagcgca tcgactcccg ttgccgcggt tacttttggt
    32761 ggtacagttt tcgtaaaatc agagtcaacg attgagatct gtggcatgaa aacgagatcc
    32821 ttcatcatca ttttgactgc tgtttttgta ttggtaataa ctgccacatc ggttacttcc
    32881 gaaccagttc cagcagttgt tgacactgca atcagcggaa tgcccgcttg cagctgatca
    32941 aaatttcttg catgaatcgc ttcgagactt aattgattag cagccaaaac ggctaccacc
    33001 ttggctgtat ccagacagct gccgccacca aaagcaatga tatgatcaca ttgatgcttc
    33061 tgaaaaagct tcagcgcatc ataaacattt tcttgcgtcg gttcggcttg aatgtctgta
    33121 tactccgcta attgatcatc tgtttttccc aacagctcct tgatcttttg aagatgcccg
    33181 gtctgttcca taaccgtatc actaatgatt aacggcttgt tgcctagttg ttctgattct
    33241 tccataagaa attggattgt tccgccgcca attcttaaat tggcaggcat tttcagttga
    33301 atattcacag aaaaaacctc tctctggctt atagcctata acagaatcct cgaagctctt
    33361 taacagagct tcgaagcaaa tctttaaatc tcactttgcg cataacgtgc tgtaacaact
    33421 ttccgatggg tataaaactc aacactgtct ttgccattgg catgcaaatc accaaagaaa
    33481 gagtgcttcc agcctgaaaa ggcaaaccaa gccatcggtg caggcacccc aagattgacc
    33541 cccagcatcc cagcatcaac attttctctg aagtagcgaa tcgctgatgc gttgttggta
    33601 tacagacacg aaccattcgc aaattcagat tcattcgtca gctcaacggc tgccatcaaa
    33661 tcttttaccc gcacgattga tgcgaccggg gcaaaaatct catcctgcca gatcttcata
    33721 tccatcgtga cattatcaaa aatcgttggt ccaacaaaat agcctttttc tggaatgttt
    33781 tcgcgaccat ctagtaccag tttcgctcct tcatcgaccc ctttttggat atagtcaaag
    33841 gtccgctgct gtgcatcttt tcgaatcact ggtcctaaaa agaccccttc gtccaagcca
    33901 ttgccgatct tgatcgcttt tgccgcagcc ttaaattttt caacaaactg atccgcgatt
    33961 tcctcttcca aaacaatgac ggaggctgcc atgcagcgtt caccagcaga accaaaagct
    34021 ccgccaataa ggtccttgac tgcccgatca atattggcat ctttcagcac aacaacatga
    34081 tttttcgctc cagtcaacgc ttgtacccgt tttagatttt ttgttccttc acggtaaacg
    34141 tattcaccga cattttttga tcctacaaag ctgatgcctt ttaccagtgg atcttgcagc
    34201 aatgtgttga caacatcgtg ggcaccatag acaacattaa aaacaccgct aggaaagcct
    34261 gctttttcaa ccagctcaac aattttctcc atcaacaatg gtgttttttc tgaaggcttc
    34321 agcaccaccg tatttcctgc cgccagagcc atcgggaaga tccagaaggg caccatcatt
    34381 ggaaaattaa acggtgtgat ccctccgatt actccgattg ggtaacgata attcgtgatc
    34441 tcaatatcag tcgccgctgt tgataaagaa tctcccatca ttaaattggt gattcccgct
    34501 gcgtgctcca cattttcgat tcctcgttga acttctccca gtgactcgga caatgatttc
    34561 ccattttcca gcgtgatcaa acgcgccaat tcacttttat gatctgttaa aagctgatgc
    34621 agcttgaaca gataacgtcc gcgaacagga acaggaactt ttttccaagt ttgaaacgca
    34681 gcatcggcag ctttaactgc ttcggctgtc tctgctttgc tagacaacgg tacattcgcc
    34741 agcgcttctt ctgtagccgg attcattact tcttcaaact ctgttgagcg actagcgacc
    34801 caacgaccat caatataatt tcttaaaaat cttgtcattt
//
LOCUS       DAYYBH010000020        34612 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000020 DAYYBH010000000
VERSION     DAYYBH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.020"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..904)
                     /locus_tag="ACHAUG_001163"
     CDS             complement(56..904)
                     /locus_tag="ACHAUG_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8480269.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1128..3068)
                     /gene="thrS"
                     /locus_tag="ACHAUG_001164"
     CDS             complement(1128..3068)
                     /gene="thrS"
                     /locus_tag="ACHAUG_001164"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8480270.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3405..3584)
                     /locus_tag="ACHAUG_001165"
     CDS             complement(3405..3584)
                     /locus_tag="ACHAUG_001165"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8480271.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3631..5454)
                     /locus_tag="ACHAUG_001166"
     CDS             complement(3631..5454)
                     /locus_tag="ACHAUG_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8480272.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5732..8011
                     /gene="helD"
                     /locus_tag="ACHAUG_001167"
     CDS             5732..8011
                     /gene="helD"
                     /locus_tag="ACHAUG_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8480273.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8104..8751)
                     /locus_tag="ACHAUG_001168"
     CDS             complement(8104..8751)
                     /locus_tag="ACHAUG_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480274.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8930..9649)
                     /locus_tag="ACHAUG_001169"
     CDS             complement(8930..9649)
                     /locus_tag="ACHAUG_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8480275.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9780..10931)
                     /locus_tag="ACHAUG_001170"
     CDS             complement(9780..10931)
                     /locus_tag="ACHAUG_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8480276.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11148..11888)
                     /locus_tag="ACHAUG_001171"
     CDS             complement(11148..11888)
                     /locus_tag="ACHAUG_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8480277.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11892..12215)
                     /gene="rsfS"
                     /locus_tag="ACHAUG_001172"
     CDS             complement(11892..12215)
                     /gene="rsfS"
                     /locus_tag="ACHAUG_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8480278.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12272..12865)
                     /gene="yqeK"
                     /locus_tag="ACHAUG_001173"
     CDS             complement(12272..12865)
                     /gene="yqeK"
                     /locus_tag="ACHAUG_001173"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8480279.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12849..13499)
                     /locus_tag="ACHAUG_001174"
     CDS             complement(12849..13499)
                     /locus_tag="ACHAUG_001174"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8480280.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13509..13820)
                     /gene="yhbY"
                     /locus_tag="ACHAUG_001175"
     CDS             complement(13509..13820)
                     /gene="yhbY"
                     /locus_tag="ACHAUG_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8480281.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13836..14945)
                     /gene="yqeH"
                     /locus_tag="ACHAUG_001176"
     CDS             complement(13836..14945)
                     /gene="yqeH"
                     /locus_tag="ACHAUG_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8480282.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14942..15469)
                     /locus_tag="ACHAUG_001177"
     CDS             complement(14942..15469)
                     /locus_tag="ACHAUG_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8480283.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15649..16434)
                     /locus_tag="ACHAUG_001178"
     CDS             complement(15649..16434)
                     /locus_tag="ACHAUG_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8480284.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16427..17296)
                     /gene="accD"
                     /locus_tag="ACHAUG_001179"
     CDS             complement(16427..17296)
                     /gene="accD"
                     /locus_tag="ACHAUG_001179"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8480285.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17297..18676)
                     /locus_tag="ACHAUG_001180"
     CDS             complement(17297..18676)
                     /locus_tag="ACHAUG_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8480286.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18684..19103)
                     /gene="fabZ"
                     /locus_tag="ACHAUG_001181"
     CDS             complement(18684..19103)
                     /gene="fabZ"
                     /locus_tag="ACHAUG_001181"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8480287.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19128..19604)
                     /gene="accB"
                     /locus_tag="ACHAUG_001182"
     CDS             complement(19128..19604)
                     /gene="accB"
                     /locus_tag="ACHAUG_001182"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8480288.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19607..20842)
                     /gene="fabF"
                     /locus_tag="ACHAUG_001183"
     CDS             complement(19607..20842)
                     /gene="fabF"
                     /locus_tag="ACHAUG_001183"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8480289.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20861..21598)
                     /gene="fabG"
                     /locus_tag="ACHAUG_001184"
     CDS             complement(20861..21598)
                     /gene="fabG"
                     /locus_tag="ACHAUG_001184"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8480290.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21610..22527)
                     /gene="fabD"
                     /locus_tag="ACHAUG_001185"
     CDS             complement(21610..22527)
                     /gene="fabD"
                     /locus_tag="ACHAUG_001185"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8480291.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22634..22858)
                     /locus_tag="ACHAUG_001186"
     CDS             complement(22634..22858)
                     /locus_tag="ACHAUG_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8480292.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22911..23873)
                     /locus_tag="ACHAUG_001187"
     CDS             complement(22911..23873)
                     /locus_tag="ACHAUG_001187"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8480293.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23883..24323)
                     /locus_tag="ACHAUG_001188"
     CDS             complement(23883..24323)
                     /locus_tag="ACHAUG_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8480294.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24629..25594
                     /locus_tag="ACHAUG_001189"
     CDS             24629..25594
                     /locus_tag="ACHAUG_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8480295.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25651..25881)
                     /locus_tag="ACHAUG_001190"
     CDS             complement(25651..25881)
                     /locus_tag="ACHAUG_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8480296.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25967..26854)
                     /locus_tag="ACHAUG_001191"
     CDS             complement(25967..26854)
                     /locus_tag="ACHAUG_001191"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8480297.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26943..28166)
                     /locus_tag="ACHAUG_001192"
     CDS             complement(26943..28166)
                     /locus_tag="ACHAUG_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8480298.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28183..29229)
                     /locus_tag="ACHAUG_001193"
     CDS             complement(28183..29229)
                     /locus_tag="ACHAUG_001193"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8480299.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29393..31120)
                     /gene="ptsP"
                     /locus_tag="ACHAUG_001194"
     CDS             complement(29393..31120)
                     /gene="ptsP"
                     /locus_tag="ACHAUG_001194"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8480300.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31120..31386)
                     /locus_tag="ACHAUG_001195"
     CDS             complement(31120..31386)
                     /locus_tag="ACHAUG_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8480301.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31865..34099
                     /locus_tag="ACHAUG_001196"
     CDS             31865..34099
                     /locus_tag="ACHAUG_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8480302.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34247..34567
                     /locus_tag="ACHAUG_001197"
     CDS             34247..34567
                     /locus_tag="ACHAUG_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8480303.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9777 a         7486 c         6043 g        11306 t
ORIGIN      
        1 gtggaaaact acgttttgtt attctaaaaa acttatataa aaaatagtaa ggtttttatt
       61 tcgaatccat aaaatattct tggattgctt ctcgtgtttt tttcactttt tcgctaagct
      121 tttcgttttt agccatatag aaataaaaaa tgatatcgat cgtagcaaat tgggccaaaa
      181 gtgaattagt tgccgcactt cgattcgtga tttcttttgg tctagatgtt tgtacgagta
      241 catcggctat cttgctaagc gggttatttc caaattgcgt caacgcaatg atttcgatat
      301 tcatgctttt cgccagctcg gctaatgcga ctacatctgc acttctacct gagtttgaaa
      361 caagccaaag cactttcttt ttctcattgc tgacaagttg cgggagcaag acgtggatat
      421 ctttttcaaa aatgattggc ttaccgacac gagaccattt ttgcagaata tcttctacta
      481 ctaaagaaga ggcgccaacc ccatataaaa aaacagtctc tgccgattcc aaacactcta
      541 cgactttttg gatcgtttct tcttctagaa tatctactgt ttcttttatc gctacttgtg
      601 cattgttacc aagtttgttt ttgatagagt aaaaggactc atttgcttct atatcactaa
      661 aaccttcatc gattccagtc tgtagttctg ttgaaatcgt gattttgaag tccgtcagac
      721 tttgaaaacc gatttttttg gaaaaacgta caactgtcgg cgcactcaac ccggaagctt
      781 ctgctaattc attagctgtc attgtaggga ccttctcaat attaccgata atgtagttgg
      841 ccaacttttt ttctgctgaa gaataatctt ccatcatact ttcaatacga ctaattacgc
      901 tcataagttc ctccaccctt ttaatgatag tctatgtaag tataccatat cagaccctta
      961 ttcacggtcg tatactggat aaaaaacaaa aaaacaatcc gcccaacaga atatcttatc
     1021 ccatcaatcc cacagattct attgagcatc tgtgtctttg aggaatacct gttatctatt
     1081 gttcggattg ctttctatct actacttttt tacaggaaat ttacctatta gttttctctg
     1141 ctgtaatttt tgacttctgc ttccatagca tcaataaaga tattcaaatc ttctacagct
     1201 gtttctttac ttccataacg acgtacgttc actgttgcat cttccaattc cttgtctcca
     1261 acaactaatt ggtaagggat cttttgtgtt tgagaggcac gtatcttata gcccattttt
     1321 tcattacgat catcaacttc tacacgcaac cccttcattt gcaaacgttc tttgatttca
     1381 taagcataat cgccatgtgc atcaacagaa acagggatga ttgttgcttg tataggtgcc
     1441 aaccatgttg gaaaagcgcc tttgtatact tctgttaaat aagcgacaaa acgttccatt
     1501 gtagaaacaa ttccgcggtg gataactact ggacgatgcg tattttcccc gtcttctcca
     1561 acatatgtca aatcaaagcg ctctggcaat aagaagtcta attgtatagt tgataatgtt
     1621 tcttctgtac caagagctgt tttgacttga acatctagtt ttggtccgta aaaggctgcc
     1681 tcgccttctg cttcaaagta atctagttct aattcatcca tagcggcttt cagcattgct
     1741 tgggcttttt cccacattgc gtcatcatca aaatatttat ctgtattgtt tggatcgcga
     1801 tagctcaagc ggaaacgata atctgtaata ttgaagtcag catatactgc caccatcaat
     1861 tcaagcgtac gtttaaattc atctttgatt tgatcaggac gaacaaatgt atgaccatca
     1921 ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag cacctgattt ttcatagcga
     1981 tgcatcattc ctaattcagc gatacgaatt ggtaattcac gataagaatg gatcgtgttt
     2041 ttgtagacca tcatgtgatg agggcagttc attgggcgaa gaacaagcat ttcgccatct
     2101 cccatatcca ttggcgggaa catgtcttct tgataatgat cccagtgtcc agatgttttg
     2161 tacaactcaa catctcccat tattggtgta tatacatgtt ggtaacctag actaacttct
     2221 ttgtctacga tataacgttc gatcgtgcgt cggattgttg ccccttttgg aagccagaat
     2281 ggcaaaccag aacctacttc tggggaaacc atgaacaagt caagttcttt tcctagttta
     2341 cgatggtctc tttcttttgc ttcttcacgg agacggatat attctttcaa agctttcttg
     2401 tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat tgtttgagtt tccacgccaa
     2461 taagcacctg ccactgataa taatttaaat acttgtatac ggccagtgga tggaacatgt
     2521 ggtccacgac acaaatcgat gaattcgcct tgttgataag cagtaatcac ttcttcttct
     2581 ggcaattcac taatcagttc tactttgtac ggatcacttg caaaaatttc taatgcttcc
     2641 tgttttgaaa gaacacggcg ttcgatcggc agattttctt tgacgatttt catcatttct
     2701 gcttcgattg ccggcaaatc ttctgcagtt acttggtttt gtccattatc tgtatcgtag
     2761 taaaaccctg aatcgatagc tggtccaacg ccaaagtgga tatttggata gaggcgacgc
     2821 atggcttggg ccatcaaatg agctgctgaa tgacggacta agccaagtgc atcttcatgg
     2881 tctggtgtga tgatctcaat acttgcatct tcctcgattg gacgagtcaa atcaacgagt
     2941 tttccgttca ctttaccagc taaagctttt tttgctaagc tttttgaaat gctttcagca
     3001 attgccaatg ttgttgttcc agactcaaat tctttgactg caccatctgg aaaagtaatt
     3061 tttatcataa tagtttcctc cttgaaatgt tttgattgat cagatcatca agctgaaaaa
     3121 caaaaaaatc ctagcatctc ttgaaaagag atactaggac gaaaagttcg tggttccacc
     3181 taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg gtccgaccgc ccttgcttca
     3241 acaagggttt cgaaagtggt catcctttgg ttttctcctg gaaaagtttc agcctagctt
     3301 ttcctctctt atcgaagact ctcaaattca gttggctttc tcatcaatca attatgtgtt
     3361 tatttaacca ctaataaata gaaaaatcaa gtgggaaact aaacttattt tttccattcc
     3421 ccatttacac cgtatgttcc ttttttcatc tcattgatga tgacatggat gtgttctttt
     3481 ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt tcatcatatt ttctaattgt
     3541 tccggactgc gaccttctac taattcgaca tgtacgaatg gcataatgct ctctcctctt
     3601 tctgatcgtt cttttttcaa ctcgattcta tcatactatg tttgtcttgg gtactgaatc
     3661 aggaaaaatg gctggatctt tttttgctct caaggcactc aaaatagaga ccgtatccac
     3721 gacttcttgc aaaacagctc cgaataatgc ggggattagt cccgtacttg caattaacat
     3781 caaaaaaata caaataaaga tgccaatcag tacagactgt ttcgctattc tcatcgtatc
     3841 tcttgaaatc actactgctt ttgctacacg caagagatca tctttcaaga tgaccgcatc
     3901 tgcactttca ctagcagctg tagaaccatg agcccccatt gcaataccga tatctgcagc
     3961 agcaagtgct ggtgcgtcat tcacgccgtc tcctaccatc gcaacaggtc tttgatcttt
     4021 agggatactt tctaaaatgg tcagtttatc ttgaggcaag ctacgggcat gtacagaatc
     4081 taattggact ttttctgcaa tcgtttgagc aatggcagat ccatccccgg tgatcatcat
     4141 caaatttttc acatcaagtt ttcttagctc cttgattgtc tttgctgctt ctgggcgaat
     4201 ctgatccgaa aataggatcg ttcctgcaaa agatcgatca attgttacat agattgctgt
     4261 attggacgtt tctatttctt cagctgaaac aaaagaggca cgtccaatct ttacttcatg
     4321 attattgact actgcttgga ttccttgacc ggtcacttct ttcagttgac taacaggata
     4381 cagttggctg tttttcacgt tgttcactaa cgaacgagct aagatatggc tagattcctg
     4441 ttctgcacta gctgcataca gaagtagctc ttcttttttt attccattga ctggctgaat
     4501 cgcctctact tccaatatcc ctttggtaag cgttcctgtt ttatcaaaag caatcgtttt
     4561 tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca acgatcccgt ttttacttga
     4621 acggctcatt cctgcaacta aagcaatggg cgcagctaga atcagtggac aaggtgatgc
     4681 cactaccagt acctcagcaa atcgtaaagg gtcttttgag aaccaccaag aaataccacc
     4741 gatcaaataa gcaatcaacg tgaaagggac tgcataacga tcagctaaac ggacaaatcg
     4801 ggcaggcttt tcttttgatt cttcgactag tttaataatc atttgatact ggctgtctgc
     4861 ggcacgtttt tcagcgatca tttggatcga tccgtctcca tttaaagacc ctgacataat
     4921 ctgatctcca ggtttcttgg tttccgggcg ggactcacct gtcaaagaag attcatcgac
     4981 cgaagattct cctttggtca ctcgaccatc tgcgggtaca agttcgccag gcttgactag
     5041 taactggtca tcaatattta tttcatcgat tactatatcc tgcaattttc cttctttgaa
     5101 tcgatgggca attcttgggg tgttatctaa caaagactgc agttctttac ccgctcgttt
     5161 attcgcgtaa tcctctagac tatctccgcc cgtaagcatg atcaatatca tcaaactagc
     5221 ccagtattct ttgacggcta atgttgcaag gattgcggta atcgctaata catctacgcc
     5281 atatcttcct gaacgtaacg tcttgatcat ttccaaaaac atcaagaagg cgagaatccc
     5341 tccagtaaat gcgacaatcg caaatgcaag ggccggctgg tgaaaaacaa attcacttaa
     5401 taaagctatc ccaccaataa gcaaagtaag taaaaatttt ttgaaatgtg tcattgtttt
     5461 ttctcctctt ttatgtgact tagagaggtt agtaataaat ttccagctgc ctctttcatc
     5521 ttaagcatag tccttgacta gcacttttta aaacaatagg ccttaattga aaacaactat
     5581 cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa aaatattcag caaggattac
     5641 tttttcgatt catcagttaa aaagtacctt ttttcgaaaa actatgctat acttttaatt
     5701 cgttatttac ggcggaagga gcaaacatgc gatgaatgag aaacaactag aacaaacgca
     5761 cgtggatgaa acgatcgaac taatccagat cgaacaaaat atgctggata caaaacaaca
     5821 agaattgact caggaaatga atcattcgac aaaagacaca gctaatcaca agattcgagc
     5881 tggttccaat gaagcttttt atgaatctgc tgttgaatat cgccaacatg agcaggaatt
     5941 gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc ctgcaaacgt tgtctgtcat
     6001 gaaagatagt ccttattttg cccgtatcga ctttaaagaa gaccgtgaaa cagaaacatt
     6061 atatctaggt atcgcttctt taagagatct ggcagaagaa acaatcgtta ttgactggcg
     6121 cgcaccaatt gccaatcttt attatgaagg tgagattggt cccgcatttt atgaaactga
     6181 aacagataga atagatgtcg atctgcagtt gaaacgacaa tttaaaatcc aagaaggacg
     6241 tattttatct atggtagata cgtcggaagt aatcaatgat gactttttgc tagagatatt
     6301 ggatgaatca tcaagcaacc atatgaaaaa catcgtctct acgatacaaa aagcacaaaa
     6361 tgcaatcatt cgggacacaa caagtaaagt aatgctgatt gaaggaatcg ccggaagtgg
     6421 aaagacatcc gctttgcttc aacgtattgc ttttctgttg tatcataatc gcaagtggct
     6481 cgaggctgaa aacgttcttc tattttcacc caaccatttg ttttctgatt acatctcgac
     6541 tgtattgcct tcattaggtg aaagcggcgt gcctacacag acattcaaaa attatttggt
     6601 gcagctgctg ccagagtttt ctttactaga tgaacagcaa caagaaagtg gttttttgct
     6661 aggggaaaaa gatccgatac aagtgatgaa aagcggattg accttagtcg atcagattga
     6721 tcgctatatc caaggcatca caaactacgg tcccttgttc cgtgatatga aaataaatgg
     6781 ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat aaagaaacaa atgaacaatt
     6841 acctttacat catagacttt ctctattaca aacgaagctc ctgaaaaaat taggcggtct
     6901 gcaaaaagac gaaacacgtc agcaatgggt caaagatctg gcggaagaac agctccaaga
     6961 actatacgca acagatccaa atcttgaata tacagaacaa aaggagcgtt cattacgtaa
     7021 aaaactggcc aaccaaatcg tacgtaaaag attccgtcga atccatcgag ggatcatgaa
     7081 ttatcaattt gtcaatataa cgaaacaata tctccacttt ctacaaactg tgccactttc
     7141 tcttctaaca cgacataaaa tcagcgagac caggtggcag gaacatttga caactgttcg
     7201 tacacacttg cgtcaaagag aattgcgtca agaggatgct gtactattct ttttactgat
     7261 gcgccgaaca tatccagtga cggttacaca caaagctcgc tatatcttta ttgatgaaat
     7321 gcaggatttc gcaccatcac aagtggcatt gctgcaagcc ttgttcccaa aagcaaattt
     7381 gactttctgt ggagatctaa atcaaaatgt ctttggaaat gaaacgatcg ttggatcatt
     7441 ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa ttaacgacaa gctatcgctc
     7501 cacaaaagaa atcactgatt ttgctaatca ttttattgtg aaagaacatc aagtagaaac
     7561 aacagcaaga aaaggagacc ttcctttgct catccaagga acaacagatg atatcaaatt
     7621 gaattggtta gcagatacca ttcgaacgac tcagggaaaa gcccggtatt ggcgtacagc
     7681 aatcatagct aaaacacaag aagaatgcga agctctttat gctcaactgc cagacgagtt
     7741 gaaacaacaa gtccagctga ttttaaatga gagtgacttt atgaagcgtt ccattatttt
     7801 acttccagcc tttttagcaa aaggtctgga gtttgaccga gttttcttgt gggatgtaga
     7861 tgcagaacat ttttcttctc agcaagacaa attgatcctt tacaccatgt gtacacgtgc
     7921 gatgcatgaa ttgacccttt ttacctccaa taatgaaagt cctttgttaa acaatatgga
     7981 tatttcatct tatgagaaaa aagtactgta agtaaaaaga caactgctat cattaatgtt
     8041 agcagttgtc tttttattta caataataat tgcagaaaaa gtaattcaat catttaaggt
     8101 catttattct atatataaat ccaaatctgt ttcaggatat tttaatttga caagcgaaat
     8161 aatcattttc agttcttcca tgttttctgg atattctggg tcaaatacct tgctatgacg
     8221 gccaatcccg ccattgtcga tcaatcggta aattcgattc cattcaatga attggtcaat
     8281 tgattggaaa ttgctgattg gatagccgcc acaatccaaa atctctgata cacgaaatgc
     8341 taggtatgca ctatcgatca ctttattttt tttcgtgtct atcttctcat ttctttcgat
     8401 aagaattttt tcaggacgat gtttcaagct ccactctaat agtagaaaga aagcctccat
     8461 ccgatactct tcttcaaata accgaaaggc tgaatcagaa cgacgaaaaa gcagcggaat
     8521 cagataattt tgtctataaa tcagcattgg tgcgcggtcc accatacact ctaaataacg
     8581 gttcagatac cctgtaccga catagtttcc taatcgatag ccttcaacgc cgaatcttgc
     8641 gtatttttgc gctaattttg aacaatcagt tacgttcaca gctaactctt ctactttgcg
     8701 gtaaccgctt aagatacgtt caaaaggttc taaagtgatt tttgcttcca tattcttccc
     8761 cgtccatctt ccacatactt catatcattt cacatgatac ttatgctctc atcataccac
     8821 aggttgaatt agaaaagaaa ataaatcaca caaaaaagca agacaatctg ttagtgagat
     8881 tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa actggttgct tagtcttcta
     8941 atgccacatt atggaacaca tgctgtacat cttctagatc ttccagtgca tcaatcattt
     9001 tttcaaatgt ttcttgatct tctggagata gtgttacctc gttttgaggg atcatttgga
     9061 tctcagcgat tgaaaaatca gtgattcctt tttcttttaa tgcttcttgt atactatgga
     9121 aatccgctac ttcaccgtaa acgatgattt gtccttcttc ttccattaca tcgcgaacat
     9181 cgatgtcttt ttccattagg tattcaagga tctcgtcagc gtcttctcct gcaaaagcaa
     9241 agattgctgt attgtcaaac atgtaagaaa ccgctccgga aacacccatg ttcccgccat
     9301 ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt cacattgttc gtcaatgtat
     9361 caacaatcac cattgagccg cttggtccaa atccttcata gcgcaattca gagtatgttt
     9421 catctcctgc accttttgct ttttcgattg ctcggtcaat aatatgtttt ggtacattat
     9481 aagttttagc gcgatcaatg acgaaacgta gtttttggtt tgcatgcgga tcaggatcac
     9541 ctgattttgc tgccgcgtag atttcaatcc caaattttgc ataaatccgg ctattattag
     9601 catctttagc agttttcttt gctacgatat ttgcccattt acgtcccata ttttcacttc
     9661 gctttctgat ttgatagttt cataacgcaa aaacgtcctg ttccattata cttgtgaatc
     9721 acgattctgt gaagcttttt ttctttcttc cttcattctt ctcattttta gtcaattatc
     9781 tatactcgga ttgggatttg cccgaaattc tgttccggaa tattgcctcc caattgatag
     9841 atttgatcac ttctaactaa cagttccgct tcttgtgcct ctttttttcc gattcgggaa
     9901 taaatagaaa tctctttttt ttcttttaag aatcttcttc cagcatcggt aaatccaagt
     9961 agtcgaagat aatttttatt ttgttggaca ggaatctctt gctttttgat attcagcaag
    10021 acataacaag ataatcgttg gatccgtgtc catgtatatc tttttgtttt tacctttgac
    10081 acaaaatcct caaaactatc agcagttgca gctgctgttt tcatgcgcga ttctattcct
    10141 tcggtcatct gatagatatc tgtcagttcc agcaaagtag aagataacaa ccgataacgc
    10201 aaatacggcc aataagattc ccaagtctgc agcgaacaag ttgaaagatc ttcttctgtc
    10261 tgaggcggca caagtccagc tattcttttt ccttcttcta acgcatggcg aatggctgtt
    10321 gcactggcta tcctctgatc tgtgatgatt ttttcatgat agcctgcact tttacgtcgg
    10381 atcggatgta attccatagg gtggacatac ttggcatttt cctttgcata acttaatccc
    10441 aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg gatacagact gcgaaaaacc
    10501 tcagtcattt tttgcggata actatacgtt tgatcagaca gttcttgaaa tgcctcttct
    10561 atcctatctt gattctcttg gacaaagcgt ccgaaactgc tgtaatcaaa cgaagtctct
    10621 tcttctgttc caaaacacaa actgtcacag tccagtgttt gcaagatttt tactgcacct
    10681 ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg gaagctccac tactaaatcc
    10741 acaccatttt gtaaagcttc attcgcgcga tgccatttat cgatgatagc cggttcgcct
    10801 ctttgcaaaa aattcccgct cattacagca atgacgacct ctgctcctgt tatttttcgt
    10861 gccatttcca catgatagcg atgtccattg tgaaaaggat tgtactctac gataatcccg
    10921 cagcttttca tgcgcatcct ccttcatagt tatcgataaa aaagaaatag agacaagtaa
    10981 aaaaaggcaa aaatcggaaa aaatacccga actatataaa ctattctcgc aaacacaata
    11041 cttctgcgac gaaaagctgc actgcaagag caatgcctat atcgttcgga tatataattt
    11101 atgttgaaga tttattcttt tcttgttaac tcgagcaggc aaaaccgcta gtctttgtat
    11161 gctacaaaga accagcgctt tgatttctct gatgctgctt cgtctgtaaa atcagaaaat
    11221 gcttcgactt tcgaaaagcc gctgttttcc agcattctta aataactttc caatggatag
    11281 gtacgctctt gatgtagttc atcttcacga ataaatcgac cagacccttc ttgttcttca
    11341 acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc ttcctggata actatcccac
    11401 aaaaaagcaa aattatcccc ttgataatga tagctatatt ctggaaaaac ctcatctact
    11461 tgataggtgg agtgtacgtc aaataaaaat cggccatttt cttctaacgc ttgatagact
    11521 tcatcaaaca cttgctgtac ctcttgtcta tttttcatat agcacagcga atcagaaaaa
    11581 caggtaattg cctgatattg tccgatttct gataaatcga gcatattgcc ttccacaaat
    11641 tggatcgatg cgtcttcttc atatgctcgc tggctggcaa tcatcaacat ctcttctgaa
    11701 aggtctaacc ctgtcacttt atatccatct tttgcaaagg caaccgctaa tgctccagta
    11761 ccgcatgcca gttccaaaat attttttgtg tcttcttgta aatgacgttt cgaaaaagct
    11821 aaccattgct gataaagtgt ttcgtccatc acttcatcgt aaatgaaagc aaatgtttca
    11881 taagccatat gttaatccaa ccattcacta agatcgacta gcggagcatc tgaccatagt
    11941 ttttccagat tatagaaatt tcgttctggc gcatggaaaa catgtacgat cacatctcca
    12001 agatcgatca atacccattt tccgccttct tttccttcaa tacgttttac ttcatatttg
    12061 ttttcttctt ctttatcaat gatttcttct gtaattgcgt tgatttgacg ttcgctattt
    12121 gctgaacaga tcatgaaata atctgccaac aaggaaacat ttcgaacgtc taaagcgata
    12181 atgtcttctg cccgttttga atctgctgct tcaatcgcaa tttttaattg ttctttacta
    12241 tctatgatga tttcctccta atatgagcag cttatttttt tgctacccag taattatatg
    12301 tttcgattgt ttttggataa atctgttgtt cttgcgcaag caaatgggcc aatgtatgtt
    12361 ttgtttcaaa tgctacagct tcgtctagat cagcccatgc aatggctcgt gcatcttgta
    12421 ccccaggaaa atctcgacct ggttcaatat agtctgctac ataaatgatt ttgtcaagga
    12481 gactcatctt agcagcccca gtcgtatgca cccggattgc atgaagaatc tctgcatcgg
    12541 tgattcctaa ttcccgctct acaaacgaag cgccaacaag accatgccag attgcattat
    12601 tataattcaa taactccgga tcaaaaccgt ctcttacgat taccattttg aactcctcat
    12661 ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat actagctttt tcaggagaag
    12721 caccgtattt tttggcaaga gccacggctg tttcttctac tccaagcaca tgcttgaagc
    12781 gttgttcgct cattctcatc tgtatttttt gcatcaggtt ttctctagaa taagcagtat
    12841 attcattact atagtccata ttcatacaac ccttcatttt gaatataatc aattacttta
    12901 tctggaacaa ggtagcggat agaacagcct tctttgatct tttgacgtat tttcgtggaa
    12961 ctgatgtcga tctcaggtac atctacccaa attacaggat atggagtatc cgttgtgtat
    13021 cctggtcgac gtatacccac aaagttgacc atagaagtca gctcatcgat tttataccat
    13081 tttggcaaat attctaccat atccccaccg ataataaagt aataatctgt atccggattg
    13141 ttttgcgtca attccttcat agtatcatag gtgtaacttt tcccaccacg agctaattca
    13201 atcgtttcaa tctgcaaaaa aggattgtct tccaccgcta attccaacat gttgagtcga
    13261 tgttttgcat caatcgtctg tttctcatca acatgaggtg gctgataaga aggcatcaaa
    13321 aatacgcggt caagacctaa attccggcca gcttgttcag ccatcactaa atgtgcaagg
    13381 tgaacgggat tgaatgttcc gcccaagatc cctacttgct ttttcttctc taagaattga
    13441 ggcatttctt caggaaataa ttgtgaacga acaaatgtct tagcttgcgt attcatcatg
    13501 gtcactcctt atattgcttt tacttctttt gatagccttt gatacttttc ttttgtggaa
    13561 ggtttgtata atacaataac acgtccaatg atttggacga tttcgcaatc tatttctttt
    13621 tctaatactt gagctgcttc ttctgcaatc tcatctgtat tttgaagtaa cgatactttg
    13681 atcagttctc ttttttctaa agcttcttca atttgaacga tcattgctga attgatccca
    13741 ccttttccga tttgaaaaat cggctgaaga tgatgcgctt tgctacgtaa aaaacgcttt
    13801 tgttttcctc ttaaactcat aattttcctt cttccttaaa tcaatgcttt gcgtttcaat
    13861 acatctacac cctttggcgc ccagcccgca attactactg gcgctgttac agtgatccag
    13921 cctagaccgg caaaaacgat gtctgttttt tctttcacag aaaactcaaa acgaacaagc
    13981 tccggaaatg cctcgacttc atcaggacga ggaggctgta ataatccacc tacatgtttt
    14041 tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa tatttacatc atttgacaca
    14101 taagcaataa atgaagcacg attgccgctg ataaaatcaa atcttgcaag tccgcccaag
    14161 aaaagtgttt gcccttcatt cagctgataa actttaggtt tgatttcttt taacggtgca
    14221 atgatccgca aatctttttt acctaagtag tgcgccattt gatggcgatg gataattccc
    14281 ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct cgattttatc caaggtcgtt
    14341 cctggaaact gcgaagttgt gattacatct tgtactcccg ctgtattttt gatgatttga
    14401 ttgataagcg tcgattttcc gacatttgtt actccgacga tatagacatc ccgtccatcg
    14461 cggtattctt cgattttttc taatagttcc gtcatctcat gagatttctt agcactagtt
    14521 aataatacat ctactggacg aattcctgct tcgtaagcac gttccttcat ccattgtgtt
    14581 aatttagttt ttttcaatga tttaggtaat atatccactt tgttcccaac taaaagtact
    14641 ggattgtcac cgacaaaacg gtgtagtcct gggattagtg aaccattgaa gtcaaaaata
    14701 tccaccacat tgacgatcaa tgcatctttt tgccctaatt cattcagtaa gcgcaaaaag
    14761 tcgtcatccg tcaatgagac atcttgggtc tcattataat ggcgtaatct gaaacaacgc
    14821 tgacaataga cttccccgga agctaaacct ttttccaaag ccgtttgcgg cgtgtatcct
    14881 aattcaccgg gtttgtttgt ttgtataacg gcaccacagc cgatacattg taattcttct
    14941 gtcattcgat tccacctcgc cagcccatat cagggtgttt tgctaataga taggccataa
    15001 ttcttttttc ccaaaaacga ttaaattgtg tcttccaact atctgttgcg ataatgggtt
    15061 ttacaaggat gctacgtatc cctgcacgat tggctccacg tatatcagtc ataatctgat
    15121 cgcctaccat tactatctca tcatctgcta aatccaattg tttctttgct atgttgatcc
    15181 ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag atcaaatttt tcaatcgctc
    15241 gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac agggattcct gcatttttca
    15301 tttctaaaat ccaagtaagc aactcttctg ttccatctgg attattccat gcaatcaacg
    15361 tattgtctaa atcggtcaat actgctttga tgcctagatt ctttaattgg gcaggtgtaa
    15421 tctgatagat ggcttctacc atccatgttg gtttgtaatt tgaaaacatg ctgtctcctc
    15481 atctgcaaaa aatgaaggag cacataaagt actcctcaac cccgtattat actgcatatc
    15541 agcaaaaaaa actacctcta tactcgttcg tctttcagaa aaagcatttc tttacaaaat
    15601 gaccaaaaat aaagattgat cacagaaaaa tttagaaaca aaaaacaact aatattggcg
    15661 gaacctcttg tatcgttttt ccagtaattt atgctgatct aatcgaacaa gttccgtcaa
    15721 tctttcaatc aacgcttttt tcataatgtg tgcgacttta tatcgtggca actgctcgcc
    15781 gttgaacgtt tcaggaataa ctcgatcaac gattcctaac tcgcgtagtt ctggcgcagt
    15841 aattttcatc aactcagctg cttctgcggc gcgactaccg tctttccaca aaatcgacgc
    15901 aaatccttct ggagaaagca cagcataaat cgtttcttcc agcatccaga cttcatccgc
    15961 taaacctaga gcaagggcac cgccacttcc accttcacca atgatgatgg cgatgatcgg
    16021 tacctttaaa tcagacattt cgagcagatt tctggcaata gcctctcctt cgccgcgctc
    16081 ttctgctcct acaccgcaat atgcacctgc tgtattgatc aaggtaatca ccggtctatt
    16141 gaacttttcc gcttgtttca tcagtcgtaa agcttttcga tagccttcag gatggggaga
    16201 accaaaattt ctgcggatat tttcctgcag atcccgtcct ttttgaatac cgatcactgt
    16261 taccggtttg ccatctaatc gagcgattcc accaacaatc gcttcgtcat ctgcaaataa
    16321 acgatcacca tggcattcct cgaaagaatc gaagatctct tcaaaaaaat ccaagcttgt
    16381 ccatcgttct ttagctcttg ctaactgaac gacatcatgt gctgtcttat ccacgatcta
    16441 tccatccttt ctgtgtatgc aattcaagaa gcgtatggat tttctgtttc aattccatgc
    16501 ggggaacaat ctgatcgaca aaaccatgag ataataagaa ttcagccttt tggaaatctt
    16561 ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct tccagcaaaa ccgatcaagc
    16621 tttggggttc tgctagaata atatcgcctt ccattgcaaa actggcagtt actcctccgg
    16681 tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc attgctatgc cgtttgacag
    16741 cagcagagat ttttgccatc tgcattagag aaaagattcc ttcttgcatc cgtgcgccac
    16801 ctgaagctgt gaacaagacg actggaagac tttcctttgt tgctcgttcg aataatctgg
    16861 tgatcttttc tccgacaatc gttcccatac ttcccatgat aaaattagga tccatcactc
    16921 caataatgaa gggaatcccc tgaatggttg ctttaccagt gaggactgct tcatgaagtc
    16981 ctgttttatc ttgcattttt ttaatttttt ccgtatattc gggaaatgct agcggatctt
    17041 tggttacaag atccgtatcc cattcttcaa aacttttttc atcaataatc aaagctagac
    17101 gctgccatgc accgattcga aaagcgtatc cgcaatgagg gcatactttt tcttcaccga
    17161 tgtctttcgt ataaaggata tgtttgcaat ttggacactt tgcccacatg ttatcaggaa
    17221 cagatggcga gttagcaggt ttgctctctc tattaggatt gatccgaata tatttctttt
    17281 tcttaaacag agccatctat gcgcctccta tctccgtttc tggtgtccaa ttcggtaaaa
    17341 attcttcttg taaaaatgct gtgctgtaat cacctgctat gactgcagga tgactaatca
    17401 aatccatctg aaattctgcg ttcgtgatga ttccttctgt gataaattca cttaaggctc
    17461 gctgcatctt cattaatgct tcaaaacgcg tatttccatg gacgattatt tttgcaatca
    17521 ttgaatcata atatggcggg atcgtatatc ccgggtaaac tgcactgtct actcgcagtc
    17581 ccatcccacc tgctggaagc agtaagtttt ggatcttccc tggagaagga gcaaaatgga
    17641 aagcaggatt ttctgcatta atccggcatt cgattgcgtg tccttggaat actacttctt
    17701 cttgacacac attcaaaggc tcaccggctg ctatctccac ctgttttttt actaaatcga
    17761 ttcctgtgac catttctgtt actggatgtt ccacttggat tcgagtattc atttccatga
    17821 aataaaaatc gccttcttca tccatcagaa actcgatcgt tccggcattt tcataattga
    17881 ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc tctttttgtt tgtgagatca
    17941 ctacagaagg agattcttcc agtacttttt gattgttgcg ttgtaaagaa caatctcgct
    18001 cgcctaaatg aatgacatga ccaaatcgat cacctaagat ctgaacttct atatggcgag
    18061 caggataaat gattttttcc aaatacatat catcatttcc aaatgccgct gctgcttctt
    18121 gctgagctga agaaaaatgc tgtgggagct cttcttttga ttgaactttc cgaatgcctt
    18181 tcccgccacc acctgcagct gctttcagca tcactggata accaattctg tctgctattt
    18241 ccaatgcttc ttctactgta gataatacac cagtacttcc tggaatgact gggacatttg
    18301 cttcttgcat caattgacgc gcattgatct tatttcccat tgcatcgatc gttgcacttt
    18361 taggaccaat aaatgtgata ttgcactctt cacacatgga ggcaaatcgg ctattttcag
    18421 ataaaaaacc aaatcctgga tgaattgctt cggcttttgt cacgattgca gcgctcagta
    18481 cttcctgaac attcagatat gaatcaacgg cttttgccgg gccaatgcag attgcttcat
    18541 ctgctaactg tgtatgaagt gcttcggcat ctgcttctga atatatcgct acggtttgga
    18601 tccctagttc gcggcatgca cgaatgatac gtacagcgat ttcaccgcgg ttagctatca
    18661 atactttaga aaacatattt cacctatcca atcataaagg ttaattccgc ttcggctact
    18721 tttttaccgt ctactttagc gattcctttg cctattccgg ctgaagcttt cactttcaat
    18781 atctcaactt caaggtataa ggtatctccc ggtgtaactt tcttacggaa ttttgctttg
    18841 tccaatccgc cgaaataagc cgtctttccc ttgaattgtt caagagaaag taatgcaact
    18901 gctcctgctt gagccattgc ttcaacaatc aatacccctg gcataacagg ttcttctgga
    18961 aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt tcttagcaac gatccgttct
    19021 ccttcaacca tttcttccac tcgatcaatc aaaagcattg gataacgatg tggaatgatt
    19081 tcttttattt cttgtatgtt cattgttttc cctctttcct gctttgctta tttttgctga
    19141 atcctaaaga gtggctgtcc atattcgaca actgactcgt tttctacaag gatttctgtg
    19201 atcactccgt cggcctcact agttatctca ttcatcagtt tcatcgcttc aataatgcag
    19261 ataacttctc ctttggcaac ttgatcacct acttttttga aagctggttt gtcgggcgct
    19321 ggttgcagat aaacgattcc cacgattggc gaaacaacct cgattccatc aaatactgcc
    19381 gattcttttt ctttcattat ttctggagca gttgttgctt gttcaggtat gacattgact
    19441 ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat tcatgcctcg acttgtttta
    19501 tttttattca tatacaattc aaattgacct tcacgcaaat cgaattccgt tagcgaggat
    19561 tgatcaaatt gactgactaa ttctctgatt tcattgatat ccaaaattat tcctcccagc
    19621 gtttcatgca cagaaccgca ttatgtccgc caaaccctaa tgaattactc attgcataag
    19681 taaaacttgc aggtttgctt tcgtttttga caactgtcaa gtcaatctca ggatcttgtt
    19741 tttctacatt gatcgtaggt gggataaatt gatgttctaa tgccagcaaa gttgccaagg
    19801 cttcgatccc gccggctgct cccaataaat gtccagtcat cgatttcgtg ctgctgacgt
    19861 aagtatctct gaaattttca cctaatgcga tttggattgc ttttgcttct gcctggtcat
    19921 tggctggtgt acttgttccg tgagcattga tatatccgac ttgttccggc tgtacaccag
    19981 cttcttccat cgcaagcttc attgctttag atgcaccaga accatctgga gtaggagctg
    20041 tcatatggta agcgtcacag tttgcaccgt atccaacgat ttctccatag attttggcac
    20101 cgcgagcttg tgcatgttct aaagattcaa gaactagaac gcctgctcct tcacccatca
    20161 caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg attaggatca gttgattctg
    20221 agagtgctgt taaagctgca aatccagcaa tccccatctc acaaatcgtt gcttctgttc
    20281 ctccagcaag aatgacatca gaatagccat gtttgatgtt acggaatgct tccccaattg
    20341 aattcgttcc gctagcacaa gctgttacga tagctgtaca aatacctttt gcacctacac
    20401 gcaaagcaat atttccagct gccatgttgg caatcgccat tggtacgaac attggagaaa
    20461 ctcgttttgc acctttttca tgtagacgaa taacttggtt ttcgattgtt ggaagaccac
    20521 cgatcccaga tccgatgatg acgccaaacc ggtcttgatt cattttttct ttatctaaac
    20581 cgctcatctc cattgcttct aaagcagcgt agattccata gatcgaaaac atatccattc
    20641 gtttgctatc ttttttaata aaatatttat cgtaagggaa atctttgact tcacccgcta
    20701 aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg tgcgatgcca ttcgtcccct
    20761 cttttaagct attcaaaaag ctgtctgcgt catttccaat aggtgaagtg actccatagc
    20821 cagtaattac cactcgattc atttgtttgc tctccttttc ttatccgtgc atgaccaatc
    20881 cgccatcgac attgatgact tgtccggtga tatatggatt ttgtgctaag aaaactgctg
    20941 tttgtgcaat atcttctact tgtccgaaac gctgcatcgg aatttgcttt tccgcttgtt
    21001 ccttgacttt gtctgctaaa acttccgtca tatcagttgt gatgaatcct ggcgcaatgg
    21061 cattacatgt aatgcctcta gttgcagctt ctcgtgccac agattttgtt aatcctacta
    21121 cgccagcttt actagctgca taatttgctt gaccaatatt tccaatcaac ccagatacac
    21181 tagagagatt aatgatggcc ccttctcgct gcttcatcat ttttttcaat acgtgctgcg
    21241 tcatattgaa ggttccaatc aaattgatat ccagacattt tttgaaatct tctgcatcca
    21301 ttcgcatcac aagtttgtca tttgtgattc cggcattgtt tactaacaca tggatcgatc
    21361 caagcttttc ttctgcttct ttgatcattt gtcccgcttt ctcataatca gagatgtcac
    21421 cggaaactcc tacgcatttt acaccgaatg cttcgatttc ttctatcttt tcttttggaa
    21481 tctctccgcg accattcaaa atgatatttg ctccagcttt tgcaaaagct aaggccatcg
    21541 ctttgccgat tcctcttgta ctacctgtga taaatacatt ttttcctttt acatccataa
    21601 cgttcctcct tatagtattg ctattgtttc atcaaatgtc tgcttattct caacacgcag
    21661 aactggtatt gttttatcga tttttttcat aaatccgctt aatacttttc cggggccgac
    21721 ttcgatgact tgttcgatgc cgattgtttt taacttatgg atactctcat agaaacgaac
    21781 aggttgcatg acttgctgtt caagtagtcc cgcgattgtt tctttcttca ttatttccgt
    21841 tgttgtgttg cttatgatag gaaaagaagg ttcagagaat tgaatctgac ttaggtcctt
    21901 cgccagtttt tttgctgccg gttctaaaat agctgtatga aaaggcccgc tgacatttag
    21961 agggatcatt cttttgaacc ctttttcttt taatagtaaa acagcttcgt ctactgcttt
    22021 ttcttcgcca ccaatcacga tctgctgagg tgtattataa tttgcaggag atacgatgcc
    22081 gtacttgcta gcttcatgac agctttcctc gatcgtttca atcggcgcat tcatcactgc
    22141 aaccattttc ccgcttccag ctggggcagc ttctgtcata taagcgcctc tttttgctac
    22201 taacgcaact gcttcagtga aggatagcgc attactagct actaatgccg aatattctcc
    22261 taaactcagt cctaaagctg catctgggat gattccatgc tccttcaata agcgataata
    22321 cgcgatgctg actgttagaa tagctggctg tgtatattgc gtctgatcca aacgttcatt
    22381 ttctgtaaag caaagttcag ccatatcgta gccgagaatc tcacttgctt catcaaatgt
    22441 ctgctttaca acggcttctt catataaatc ttttcccata ccttgatatt gtgcaccttg
    22501 gccactaaat aagaatgctg ttttcatgtt tttctccaat atatccagaa tatatgtttg
    22561 attcttccta ttcaaaaagg ccgagacatt cctatttcag gaaatgtccc agcccccaac
    22621 ctaattttaa tgattatgca gtttgttttt ctacgtaagt cactaaatct tgaaccgttt
    22681 tgattccgtc ttctgtttcg attttcacgt caaattcatc ttcaatttca ttgatgattt
    22741 ggaataaatc caagctgtca gcttctaagt cttcttggat gtcagtagtc aatttgattt
    22801 cttcagtttc ttttcctaat tcttctgcga tgatttcttg aattttttcg aataccataa
    22861 taatatattc ctccgatatt gtttgctttt tatttttata atttaacagt ctacaactcc
    22921 atgagaatac ttccccatgt taaacctccg ccatagcctg taaatattac tttctgctgt
    22981 tttcctaagg tgatctttcc actagaaact gcttcatcca ataagatagg gatgcttgca
    23041 gcagatgtat tcccatattt atccatattg gtcaggaact tttcttgcgg catctttact
    23101 tttcgggcaa ttttctcaat aattctgagg tttgcctgat gcaaaagcag ataatcaact
    23161 ggtttgtctt tcatgacttc ttggatattg tttgcaacgt ctcgtacggc aaaatcaaaa
    23221 atatctctgc cagtcatttg caaataatac gaaccttctg aatcttctga atagaacgga
    23281 tttttatttt gctggtagcc agcggtcaat gagctacttc tggtaccgtc agctgctaat
    23341 ttttctttca gcaatttttt ctgtggattc gcttctaaga gaatcccacc tgctccatcg
    23401 ccaaacaaca cagcagtcgt acgatcagac caatcgatta gcttagatag tgtttcacca
    23461 ccaatcacca gtcccttagt tgagccgcag cggatcattt tttcagccat gcttaacgca
    23521 taaacaaatc ctgagcacgc agcactgata tcaaaagcga tagcatgagt tgctcccaat
    23581 ttcccttgta cttgtgctgc tacagatggt gtcgtgtaat cgggcgacat tgttgctact
    23641 aaaataaaat ccaaatcttt agggtttaga tttttctgtt ttatcaattt ttccgccact
    23701 aaaatgcata aatcagaagt ctcctgattt gttacacatc ttcgttcacg aatacccgtc
    23761 cttgacgaaa tccattcatc agaggtatcc atcatcttgg aaagatcatt attagtaata
    23821 atcttttcag gaaggacgct ggctgttgca gtaatcattc caaattgttc cattaaatat
    23881 cctcacttat agtcttgcaa gaagtcgtgc aagtttttca gtgcatccag caaggcatgc
    23941 tgttcctccg tagccattcc gtctaagatg ttcttgacca tttcacgatg aaaatgctga
    24001 tggacccgaa atagtaactt tccttttttc gttagtccca actttacaac gcggcggtca
    24061 tcttcactgc ggatacgttc gacatagcct ttttttacta acttgttaat agcagtggtc
    24121 aaggtcccaa cagtaattga tagttctttg gctacttctg aagttgtttt tttcttgtac
    24181 atcccgattg cctcgatcgt gtgcatctct gtgattgaca gatctttgaa ttgagatttt
    24241 ttcagctcag actcttcgat tgtcaaaata tcattgaaga cactaacaag ataatcattt
    24301 actgttttta aatctggttc catgttccac cgtccactaa ttagtttgat tatcaaatct
    24361 tttgatgttc aaactatatc ttaacttttt tcaaaatgca accttaaaag atcatttttt
    24421 tactatatga gcttttttta agattttact catttaaaac aactgataaa tcagcattta
    24481 taatttcttt tattagaaaa atcatctatc tcacatattt tgattatcaa agtattttct
    24541 gtgaacagaa ctatagaatt ccctaaaact tgtccattat ttcaaaattt gttatagtct
    24601 ttttattcaa agcgtaaaag gagatcctat gttaaaagaa aaaatgaaaa ctctccccat
    24661 gctgccgggt gtctatctta tgaaagaccg agatggtaag gtcatctatg ttggtaaagc
    24721 aaaacggctc aaaaatagag taagcagcta ttttcatcag aataaacagc attcaaaaaa
    24781 agtattacgt atgattcatc atataacaga ttttgatttc gtcgtagtag acacggaatt
    24841 agatgcttta ttgctcgagt gtcaattgat ccaacattac cgtccttttt ataaccgaca
    24901 aatgaattat ttcagtaatt ataattatgt acatattacc aataagggat ttgtattaac
    24961 agatactcct acggctcgaa catacgggcc ttttcgtctc tataaaaaaa tgccgtctat
    25021 tttgcgtata atggaagaaa cttatcaaat gccttggttg tctgaaatta gtttgttagc
    25081 tttgcgagtc cagcttcctg acctacagga aatgactttt gaacagaaaa aaaaggaact
    25141 ccaagggctg tttcaaggcc gaaataaaaa gctactgact tacttgaaaa aaagacaaca
    25201 acatttcatc tatcagttga atttcgaaaa agccggtatg cttcaaaaag atatcgaatt
    25261 agttacttat tttattagac ggatccagga acaaaagcag tttttaagaa caccatcgct
    25321 caccttcagc atgccattag cagcagatga gagtcagaaa aagcactacc tcgtttgtta
    25381 tggtcaactt gcagaaacaa tgattacctc tggtgacgtt tctcctgact tctattatga
    25441 aaagaaagaa gctcatttat ctttgaaacg tcaattgtca aaagaagaaa tcgatcctgt
    25501 acaaatcttg atcagttacc gcaaaaaatt agaaaaagaa cagtttgaaa ttgaactgtt
    25561 aaacaaaaaa gaagctgaaa agcaactaaa ttagttgctt ctcggcttct tttaggcatc
    25621 ttcaacttca aatgtcaatt atcctaaaat ttattggatt aagtcataga tttccatcgc
    25681 tacgatatcg atattatcaa attcgtattg ctgatgagta tctgtttcag ttaattcaaa
    25741 tgtatcggtc gctttatcat aagttactgt acacttctct acgccttctt tttcaaaacg
    25801 gcgaacttgc agttcattat ctgctgactc gaccatagcc tccagtcgtt ttatgattgc
    25861 gactaattgt gaatgtgtca tcggaggtcc tccttttctt atttctgtta tattgtatca
    25921 aaagcatagc ctacttgcat tgattttctc taaaaaaact cgttttttat gatttttttt
    25981 ctggtatttt tccttcaggc caccaaagtt tgatcaaagc ttgggttccg tcattctcaa
    26041 aacctttatc tgccaactct tcatatagtt tcaatgcttg ggtagttgct ggcaatacaa
    26101 gatccatttt ttctgcttct tctaatgcaa tctttaaatc tttgataaaa tgtttgacaa
    26161 aaaaaccagg tgtgtaatct tcttttaaaa tacgaggaga ataattgctt aaagaccagt
    26221 tagctgcact tccacctgaa agcgtctcaa tcactttctt taaatcaagt ccgttttttt
    26281 gtgcatagac aagcatttcc gtcattcctg tcatcgtacc tgcaatcatg atctgattag
    26341 ccatttttgt atgctgacct ttcccagcag aaccatgcaa tgtaaatgtc gtaccaaatt
    26401 ctttgaataa aggcagtact tgatcataaa cggcttcttc tccaccgacc atgatcgtca
    26461 gtgtaccatt ctttgctcct aagtcaccac cggataccgg tgcatctaac gcatctgcgc
    26521 ctttttcttt tgctgttttt gcaatttttt ctgccaggct tggtgtactt gtagtcaaat
    26581 caactaaaat atgtcctgag atatctgcag aaaaaatccc cgactgtcca tagtagattt
    26641 cttctacatc tttggggtaa cctaccattg taaagagaat attactttgt tctgctaccg
    26701 cttttggtgt atctgcccag atagctcctt ctgttactaa aggatcagct tttgcttttg
    26761 tccgattata gacagctact tcgtgtcctg cttctagtag atggcgtgcg acagcacttc
    26821 ccatgacgcc tgttccgata aaaccaagtt tcattttttc gacctcctaa aaatcaaaaa
    26881 tccttgcttc tatgctagta tacgctagaa acaaggattt tttcataaaa aatgtttgaa
    26941 ttttatcttt taaatgaatt taattttatt ttgattcgtt caatcgagtc ttcttcttct
    27001 tttcttcgca tctcttcttc atgataaaca accatgtctt gataaaattc gatcatagct
    27061 tcgccgaaat gtgtagatga aatatcataa agtttagctt ctaaaacttt tggatcttta
    27121 ggaacttcac tggaaatata atcaataagt gctggtgcaa agtcttcgtc ggtttcaaaa
    27181 gttttcccta atgaaggatg atcgaacaat cggttcaaat aagcatttcc ttccacgaca
    27241 caaggtgttc cggctgccat cgcttctgta taagtaagcc cttgtgtttc tgaagtagaa
    27301 gcactgacaa aataatctgc cgctttataa taaagtgcta catcattgtt gtccacttct
    27361 cctgtgaact gcacaaattc ttctaactgc agttctgaga tcaattcctc taactcttct
    27421 acataaggtc cttttcctac gataactaat ctggcgttag gataagcgga gatgacctga
    27481 ggaaacccat taatcaaggc ttggatattt ttctcataag aaatcctgct taaagaaaga
    27541 atcatcaaat ggttttcctg aagacctagt gactccctta atttgctgat atcctgctga
    27601 gtgatatcag aacgtttgaa tttttcaatg tcgataccag taggaatcac gcgaagcggt
    27661 gctattacac catagctgcg taatgtatca ataacacgtt cacttggaca aactacacct
    27721 gtcgtatgat ttgcaaataa tctagacaag tattttacat gggattgcct gatcactttt
    27781 cctttaccga tataatgaag gtagtcttca tacatcgtat ggtaagtatg gatcagaggt
    27841 attttcaact tcttagctac catcttccca agtatccctg ctccgaattc tgtatgggta
    27901 tgaataagat ctagttctag ttctttggca accaaataag cataccacat gcctctaaca
    27961 acaactcttc gatccttgaa agaaataaat ggtacacttg gcattcgaat cacatctttt
    28021 tccaacttat ccgcattagg atcggtcgtt gtaaaaatat agacttcatg cccatgtctt
    28081 tctaattctt gttttaatgt tttgatcgat gtagcaactc cgctgacttg cggaaagtag
    28141 gtatcggtaa aaaagccgat tttcatgttt ttcgcctcca aactagtcct tgacctgatt
    28201 tacttttttt aatttgctag agactttttc attcagtaca ctttcgtata catttttaag
    28261 ttcttcaccg attttattga tacttcgctc ttttgctgtt tgataaccgg cttgtgtcgt
    28321 actcggtagt tcttgattgg taatctgttg gatcagtcgg ataaattctt cgtttgtctt
    28381 ccccatatag cagttcttgc tatcttctaa ccagccatta tacactggaa tatctcgaac
    28441 aagtacgttt tgtttgcttg ctaaagcttc taaaacaacg atcccttctg tctcttcata
    28501 agaagggaaa aagaatagat ctgcacctga atacgcacct tctatgatct ctccacgaat
    28561 gtaaccagga aacaggacat tttcaggatg atcttctttt acaattttcc ggatgttccg
    28621 aggaatcgag tacatgggaa catgaccaaa ccaaataaat gtgtattcgg gcatcttctt
    28681 agcaatttct acgaaatcaa tgattccttt acgttcgaaa aacaaaccga cacaaatgat
    28741 tactttttga tccggagaca gtttgaaata ttctctgaac ttttgttctt tcattggatc
    28801 agggtgatat ttctctaagt cgatcccatt ggaaatagct tgaatcggca cacggatacc
    28861 atagctttcc aacaactgtt ttgaataagg tgttggcgtg atcagatagt ccgctttttg
    28921 gtacaatcct accagatatt tttttacgat aggagaaagc tggttcgatc cgataaagga
    28981 atttcggaaa tcttcctctg tagaatgcgc atgatagaca actttttttc cttctcttct
    29041 ggctttattg accatattat gactattgat tccgtatgta ttgatgtgca ggatgtcata
    29101 atcttctttt tcatctagtg tataagagat accgacttcc gtcagagcac gtttttgatg
    29161 atcaagcgca cgcccgattc cagattttgc aagcatcttt tcactttcaa aatataataa
    29221 taccttcacc ttacgtcccc cttcacttat atagtataac atagcctaca aactgtcact
    29281 caaacttaag ccctattttt gtctattctt actgacaaaa acgttttctt aaaaaagaac
    29341 aaaaaaactg gcgtagctct tttacaagcc acaccagctt ttgatacgtt gtttatttta
    29401 catattcatg aacaagttca acgacttctt ccatcgtatc gcattctttc aatgcacgat
    29461 cagcaagttc agccattttc gctgtgtcca agcgtttcat taaactacgc gttttaagaa
    29521 tagatgtcgc actcattgag aactcatcta gacccatacc tactagcaat ggaacagctg
    29581 tttgatctcc agccatttct ccacacatac ctgcccattt tccttcagcg tgtgctgcat
    29641 caattacatt tttgatcaaa cgtaaaattg atgggttata tggttggtac aagtaagaaa
    29701 cgcgttcgtt catacggtct gctgccattg tgtattgaat caaatcgttc gtacctacag
    29761 agaagaaatc tacttctttt gcaaacttgt ctgctaagac tgctgctgct ggaatctcga
    29821 tcatgatgcc gacttggatc gtatcagata cttccttgcc ttcacttatc aatttttgtt
    29881 tttcttcttc aaagatcgct tttgctgcgc ggaattcttt gagtgtagct accataggga
    29941 acatgatacg taaattacca tgaacagatg cacgaagcaa tgcacgcatt tgtgtacgga
    30001 acatttcgtc gccttgttca gataaactga tacgcaatgc acgatatcct aagaatggat
    30061 tcatttcatg aggtaattgc agatatggta gttctttatc tccaccgatg tccattgtac
    30121 gaacaactac aggttttcct tccatacctt ctaatacagc tttgtaagct tcatattggt
    30181 catcttctgt tgggaagtct ggagaatcca tatacaggaa ttcagtacgg tacagaccaa
    30241 ctgcttctcc accattatta tggacaccaa ctagatcttt tggtgttccg atgtttgcag
    30301 ctaattcaaa atgcttgccg tcagctgtga cagtttctgc attttttaat ttttcccatt
    30361 ctgctttttg tgctgcgtaa tctgcacctg ctttttcaaa ttcagctttt tgttcatctg
    30421 taggatcgat gattacatcg ccttcgatcc cgttgacagc caaaatatcg ccttctttga
    30481 ctttagcagt aatttctttt gtcccaacga ttgctggaat ttctaaagaa cgtgccatga
    30541 tagcagaatg tgaagtacgt ccaccaatgt ctgttacaaa cgcttttaca aaattgcgat
    30601 ctaattgtgc tgtatcactt ggagtcaagt catgtgcaac aacgaccact tcttcgttga
    30661 tcattgatgg atttggtaaa gtaacgccta ataagtgcgc taaaatacgt ttagcaacgt
    30721 cacgaatatc tgcagcacgc tcttgcatat aagcattatc ttccattgct tcgaacatac
    30781 cgatatacat atccgttact tcttttagcg ctgattcagc atttacgctg ttgtctttaa
    30841 tattttgctt gatttgacct accatttctg gatctgaaag aaccatcaaa tgggcatcaa
    30901 atacttgcgc ttctgcttca cctaagcttt gcgctgcttt ttcacgaatt tgctgcaatt
    30961 cttcagtaga tttagctaat gcagcatcca aacgagcttc ttcagccgca gtatcttcta
    31021 ctgttgcctt actgaatgtt aaatccggtt gaactagcag gtaagctttt gcaacggcta
    31081 caccgtcact agcggcaatc ccttttagca tttcaaccat tattcagata agccttcttt
    31141 cttcattgtt tcaacgattg ctgccattgc atcagcttcg tcagcacctt ctgcagtaat
    31201 agtaacatca gaaccttgac ctacacctaa agacataacg cccatgattg atttcaagtt
    31261 tactgattta cctttatact ctaagtttac atcagagtta aatttgcttg ctgtttgtac
    31321 taatagtgta gctggacgag cgtggatccc agtttctgct actacgtgaa attctttctt
    31381 ttccatatta atcggtctcc tttaagtgag tgttgttttt atgtgagggt tttctttttt
    31441 agtaattcag agaatcctaa caaatgccac cctttcatct gtaaagatta acattttttt
    31501 tcgttagata caacagtttt cattcaattt cagataaaag tattcaaatt attccggatt
    31561 ctttttcatc cttttcttca cattttataa caaacgtgtt tatttccttt ttcttgtcgc
    31621 tttctcttct cctgacgttt atcattcttt cctttgaccc tctttgtttt catatgaaac
    31681 gaattacttg cttccctttt tatttttagt ataataagag tcaaaggtca ggaatggtca
    31741 atagagattt caccttttct acctcataaa tctcttaaaa aactaggggt tattctttta
    31801 cctcatgaaa aacgagatgt aaaatatgga tgtacccatt tttgtgaaag gacgtgaatg
    31861 attcatgctt tgtcaaaact gcggcaagaa tgaggcaacg attcacttgt atgccactgt
    31921 caatggtcag cggacacaac tcgattactg tcagagctgt tatcaaaaaa tcaaaaatca
    31981 acaaaatgga ggtctgacta atatggccca aaatgatcca tttggatttg gcagtttgga
    32041 cgatcttttc cgttccatgt ctcgccaaat gcaacagcaa ggaaattatg agcaaacacc
    32101 accaacacaa tttggcggaa acaacaactt caatggtggt cagccacctc aaggcaatac
    32161 agaaggttta ttgggagaat acggaatcaa tatcacagaa caagcacgac aaggtgatat
    32221 cgatcctgtg atcggacgcg atgacgaaat caaacgagtc attgaaattt taaatcgccg
    32281 aaccaagaac aatcctgtct tgattggaga accaggtgtt ggtaaaacag cagttgttga
    32341 aggtttagca caaaaaatcg tagatggtga tgtaccgcaa aaattaatgg acaaagaagt
    32401 cattcgttta gatgtcgtat ctttagtcca aggtactggt atacgaggac aatttgaaga
    32461 acgtatgcaa aaattgattg aagagatccg tcaagcagaa aatgtgatcc tattcataga
    32521 tgaagttcac gaaatagtcg gtgctggttc tgctggtgat ggaaatatgg atgctggtaa
    32581 tattttaaaa ccagctttag ctcgaggaga attgcaaatg gttggtgcta ctaccttaaa
    32641 tgagtaccgc atcatcgaaa aagacgctgc tttagaacgt cgtatgcaac ctgtacgggt
    32701 agatgaacca actgtcgaag aaacgatcag tattttgaaa ggtttgcaaa aacgttatga
    32761 agactatcat catgtaaaat atactgatga ggccatcgaa gcagccgcaa ctttatccaa
    32821 tcgttacgtc caagatcgct tcttgccgga taaagccatc gatttgctag atgaaacagg
    32881 ttctaagaaa aacttgacga tccaaatcgt tgatcctaaa acaatcgaga aaaaactgca
    32941 agaagcagaa gaacaaaaag ttttagcttc tcgagaagaa gatttcgaaa aagcagctta
    33001 ttatcgcgat cagatcaata aacttcaaaa aatgaaagaa cgtcagctca ctgaagagga
    33061 aacacctgtg attaccgaaa aagatatgga aaaaatcgtt gaacaacgga caggtattcc
    33121 agtgggtgaa ttaaaagaaa aagaacagac acaactgaaa aacttagcag atgacttgaa
    33181 agctcatgtc atcggacaag ataacgctgt agatcgagta gcgaaagcga ttcgccgaaa
    33241 ccgtgttgga ttaaacaaac agaatcgtcc aatcggctcc ttcttatttg ttggaccaac
    33301 tggtgtaggt aaaacagagc ttgcgaaaca acttgcctac gaactattcg gttcacaaga
    33361 ttcaatgatt cgtttcgata tgtctgaata tatggaaaaa catagtgtct ctaaattgat
    33421 tggttctcct ccaggttatg tcggatacga agaagctggt caattaacag aaaaagtacg
    33481 gagaaatcct tacagtcttg tcttgttaga tgaagtagaa aaagctcatc cagatgtttt
    33541 gcatatgttc ttgcaaatct tagatgatgg tcgattgaca gatgcacaag gaagaacggt
    33601 cagctttaaa gatacgatca tcattatgac aagtaatgcc ggaactggta aagtagaagc
    33661 aaacgttggt tttggtgcgg cacgcgaagg tgttactcgt tctgtattga accaattaaa
    33721 caactacttt acaccagaat ttctgaaccg ttttgacggg atcatcgaat tcagtgcatt
    33781 atcaaaagaa aacttgatga caatcgtcac actaatgcta gacgatgtca accaaatgct
    33841 ggcagcacag caacttcaca tcgaagttcc tacgaatgtc aaagaaaaat tagttgactt
    33901 aggctatgat ccatctatgg gtgctcgtcc tcttcgacgc acgatccaag aacaaatcga
    33961 agatggtatc gctgaatttt atcttgatca cccttctatc catgagttga aagcaaaact
    34021 ggataaagat ggaaaaatca tcgttacttc taaaccagaa cgtttggcta aagaatcagc
    34081 cgaagaaacg gctgaataaa tccagtttta tagcgtgaga cggtatttct ttgagaaatg
    34141 ccgtctcatt cttttttcag aaaagtcaaa atactgttca ttttataaaa aagaaattat
    34201 aataagagaa acaagcaatt aaaaatacat aaaagaggtg agcgccatga aattggtaaa
    34261 tgtaacaaac agccattccc gtcttgtatt aaaccagctt gaaaacactg atgcccatct
    34321 agtcaaggtc tacacagccg gaaataccac tattgtctac actgaagccc ctcaacataa
    34381 tgaaattctg ctgatcaatg acaaacgtaa aatccagccg aaagaaatcg aagatgctaa
    34441 aaattatttc ttagataaaa tcaaagacgc cgtttaccac gaggatgaaa tcaaagtaat
    34501 cgaattggca ggccttgttg aaatctcgat tccaaaacat gaattaggcg ctacattagc
    34561 cgtgtaaaca aattttgaac ataataatta aagagcgtgg gacaaaagta aa
//
LOCUS       DAYYBH010000021        34520 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000021 DAYYBH010000000
VERSION     DAYYBH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.021"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHAUG_001198"
     CDS             212..604
                     /locus_tag="ACHAUG_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8480304.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHAUG_001199"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHAUG_001199"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8480305.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHAUG_001200"
     CDS             2698..2943
                     /locus_tag="ACHAUG_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480306.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHAUG_001201"
     CDS             3165..4775
                     /locus_tag="ACHAUG_001201"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8480307.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHAUG_001202"
     CDS             complement(4752..4964)
                     /locus_tag="ACHAUG_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480308.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHAUG_001203"
     CDS             5067..5936
                     /locus_tag="ACHAUG_001203"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8480309.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHAUG_001204"
     CDS             6101..7372
                     /locus_tag="ACHAUG_001204"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8480310.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHAUG_001205"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHAUG_001205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8480311.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHAUG_001206"
     CDS             8763..9026
                     /locus_tag="ACHAUG_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8480312.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHAUG_001207"
     CDS             9471..9920
                     /locus_tag="ACHAUG_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8480313.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHAUG_001208"
     CDS             9977..11395
                     /locus_tag="ACHAUG_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8480314.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHAUG_001209"
     CDS             11395..12255
                     /locus_tag="ACHAUG_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8480315.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHAUG_001210"
     CDS             12429..13202
                     /locus_tag="ACHAUG_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8480316.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHAUG_001211"
     CDS             13349..13714
                     /locus_tag="ACHAUG_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8480317.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHAUG_001212"
     CDS             complement(13862..15070)
                     /locus_tag="ACHAUG_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8480318.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHAUG_001213"
     CDS             complement(15089..15367)
                     /locus_tag="ACHAUG_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480319.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHAUG_001214"
     CDS             15560..16216
                     /locus_tag="ACHAUG_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8480320.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHAUG_001215"
     CDS             complement(16658..17455)
                     /locus_tag="ACHAUG_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8480321.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHAUG_001216"
     CDS             complement(17452..18636)
                     /locus_tag="ACHAUG_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8480322.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHAUG_001217"
     CDS             complement(18661..19908)
                     /locus_tag="ACHAUG_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8480323.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHAUG_001218"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHAUG_001218"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8480324.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHAUG_001219"
     CDS             21888..22202
                     /locus_tag="ACHAUG_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8480325.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHAUG_001220"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHAUG_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8480326.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHAUG_001221"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHAUG_001221"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8480327.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHAUG_001222"
     CDS             23546..23875
                     /locus_tag="ACHAUG_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8480328.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHAUG_001223"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHAUG_001223"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8480329.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHAUG_001224"
     CDS             24863..25687
                     /locus_tag="ACHAUG_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8480330.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHAUG_001225"
     CDS             25680..26027
                     /locus_tag="ACHAUG_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8480331.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHAUG_001226"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHAUG_001226"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8480332.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHAUG_001227"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHAUG_001227"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8480333.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHAUG_001228"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHAUG_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8480334.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHAUG_001229"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHAUG_001229"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8480335.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHAUG_001230"
     CDS             31684..32451
                     /locus_tag="ACHAUG_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480336.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHAUG_001231"
     CDS             32441..34483
                     /locus_tag="ACHAUG_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480337.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11101 a         6121 c         7249 g        10049 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat
//
LOCUS       DAYYBH010000022        32977 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000022 DAYYBH010000000
VERSION     DAYYBH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.022"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            233..1374
                     /locus_tag="ACHAUG_001232"
                     /pseudo
     CDS             233..1374
                     /locus_tag="ACHAUG_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1500..2579)
                     /locus_tag="ACHAUG_001233"
     CDS             complement(1500..2579)
                     /locus_tag="ACHAUG_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8480338.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2688..3128)
                     /locus_tag="ACHAUG_001234"
     CDS             complement(2688..3128)
                     /locus_tag="ACHAUG_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8480339.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3185..3595)
                     /locus_tag="ACHAUG_001235"
     CDS             complement(3185..3595)
                     /locus_tag="ACHAUG_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8480340.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3776..4210)
                     /locus_tag="ACHAUG_001236"
     CDS             complement(3776..4210)
                     /locus_tag="ACHAUG_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8480341.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4310..5158)
                     /locus_tag="ACHAUG_001237"
     CDS             complement(4310..5158)
                     /locus_tag="ACHAUG_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8480342.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5547..7313
                     /locus_tag="ACHAUG_001238"
     CDS             5547..7313
                     /locus_tag="ACHAUG_001238"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8480343.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7415..8674
                     /locus_tag="ACHAUG_001239"
     CDS             7415..8674
                     /locus_tag="ACHAUG_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8480344.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8821..10122
                     /locus_tag="ACHAUG_001240"
     CDS             8821..10122
                     /locus_tag="ACHAUG_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480345.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10125..10976
                     /locus_tag="ACHAUG_001241"
     CDS             10125..10976
                     /locus_tag="ACHAUG_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8480346.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10989..11804
                     /locus_tag="ACHAUG_001242"
     CDS             10989..11804
                     /locus_tag="ACHAUG_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8480347.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11955..13121
                     /locus_tag="ACHAUG_001243"
     CDS             11955..13121
                     /locus_tag="ACHAUG_001243"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8480348.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13351..13998
                     /locus_tag="ACHAUG_001244"
     CDS             13351..13998
                     /locus_tag="ACHAUG_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8480349.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14082..15026
                     /locus_tag="ACHAUG_001245"
     CDS             14082..15026
                     /locus_tag="ACHAUG_001245"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8480350.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15161..16612
                     /locus_tag="ACHAUG_001246"
     CDS             15161..16612
                     /locus_tag="ACHAUG_001246"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8480351.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16633..17469
                     /locus_tag="ACHAUG_001247"
     CDS             16633..17469
                     /locus_tag="ACHAUG_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8480352.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17469..18128
                     /locus_tag="ACHAUG_001248"
     CDS             17469..18128
                     /locus_tag="ACHAUG_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8480353.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            18254..18943
                     /locus_tag="ACHAUG_001249"
     CDS             18254..18943
                     /locus_tag="ACHAUG_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480354.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18949..19905
                     /locus_tag="ACHAUG_001250"
     CDS             18949..19905
                     /locus_tag="ACHAUG_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8480355.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19949..20461
                     /locus_tag="ACHAUG_001251"
     CDS             19949..20461
                     /locus_tag="ACHAUG_001251"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8480356.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20717..21265)
                     /locus_tag="ACHAUG_001252"
     CDS             complement(20717..21265)
                     /locus_tag="ACHAUG_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8480357.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21354..22535)
                     /locus_tag="ACHAUG_001253"
     CDS             complement(21354..22535)
                     /locus_tag="ACHAUG_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8480358.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22771..24279)
                     /locus_tag="ACHAUG_001254"
     CDS             complement(22771..24279)
                     /locus_tag="ACHAUG_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8480359.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24438..24629
                     /locus_tag="ACHAUG_001255"
     CDS             24438..24629
                     /locus_tag="ACHAUG_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480360.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24832..25749
                     /locus_tag="ACHAUG_001256"
     CDS             24832..25749
                     /locus_tag="ACHAUG_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8480361.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25742..26815
                     /locus_tag="ACHAUG_001257"
     CDS             25742..26815
                     /locus_tag="ACHAUG_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480362.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26963..27481
                     /locus_tag="ACHAUG_001258"
     CDS             26963..27481
                     /locus_tag="ACHAUG_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480363.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27462..27854
                     /locus_tag="ACHAUG_001259"
     CDS             27462..27854
                     /locus_tag="ACHAUG_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8480364.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27930..28865
                     /locus_tag="ACHAUG_001260"
     CDS             27930..28865
                     /locus_tag="ACHAUG_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8480365.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28998..29777
                     /locus_tag="ACHAUG_001261"
     CDS             28998..29777
                     /locus_tag="ACHAUG_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8480366.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29770..30087
                     /locus_tag="ACHAUG_001262"
     CDS             29770..30087
                     /locus_tag="ACHAUG_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8480367.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30331..31941
                     /locus_tag="ACHAUG_001263"
     CDS             30331..31941
                     /locus_tag="ACHAUG_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8480368.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32098..32946
                     /locus_tag="ACHAUG_001264"
     CDS             32098..32946
                     /locus_tag="ACHAUG_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8480369.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        10897 a         5895 c         6426 g         9759 t
ORIGIN      
        1 gttcagatag gaaaagaaaa atatctaatt ttgtattgct ttttttcttc aagataaacg
       61 ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt ttacttaaat tgttaaaact
      121 tgaaaatatt actgtttttt tcataaattt agacaaaaaa acagtaaaca tataggtaga
      181 atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata
      241 ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac
      301 agtccacaca gaggagacag aaacagccaa atggacagaa accaagtggg ccaacgtcaa
      361 tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa
      421 aggacaaaca gcgaccatca aagcaaaaat tgactattcg ggagacttag cacctatact
      481 acaatcagct gttacatttg agaatgttga tcccactttg tcgattggct atgacgagaa
      541 cactttgact atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga
      601 cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca
      661 tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagccatcg actcaggagg
      721 agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag aagtaccgcc
      781 tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag aacccgatac
      841 agaaactcaa ccagagccag acaataaatc aaaacctgaa ataacccctg gagaaaaacc
      901 agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc
      961 agacacagat aatgaaactg aaaatccaga aaaaccagag gtagatccag aagaaaaacc
     1021 agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa
     1081 caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct
     1141 aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc
     1201 tgaaacctta aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa aaataaacag
     1261 ctaccaaaat ccggagagtc acaaaacaaa gttatactgt ggagcggaat cattctatta
     1321 agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa acagatctct ctaacatttg
     1381 aataatgtct gtgaacgcat ttttcaaata aaaagaaata tccagaaaat gaccccaaaa
     1441 taccagattg aaatctgata tttggggtcg ttcatacttt atctttcttc atatttattt
     1501 taagggatat ctgtttcttt tggaagatgg ttcgggatct ttttcacttt caatacatct
     1561 aaaaagaagg taaagatcgg aatgccgaca atcaagcccc aaacgccaaa gaaccgttcg
     1621 ctaacaagta agaccacaaa tgtataaaaa atcggcagtt ctgttttgct ggacatcaac
     1681 ttcggattca gcacataaga ctcaattaga tggacaatca aaatcgtaat caggatataa
     1741 acaacatctc ggatgcctcc ttgggaataa gctatgaaag atagaggaat acaagaaatg
     1801 ataacacctg ctactgggat cagacttaaa atgaaaatca tgatcgctaa actcagcagc
     1861 tgatgaaagc caaatacagc caaggcgatt gtagtgagca acgtatttaa aagagcgaca
     1921 acaaattgag cttctaatac caaaccaaat gtattgacaa acttatcggc aaaatagtaa
     1981 atatcttgaa agaaccaaga aaattcactt tttaaaaaca atcttgagaa tacaattgtt
     2041 tttttctttt caatcataaa aaagaaactt aagatgaatg acatagcaaa cgccactgcc
     2101 atttttccta tatcctgtaa atagccaagc gcaatcgaag cgccattttg aagctgttca
     2161 aaaaaatcgt ttcgctcaat ataagtatgg atatattgta aaacaggatc tgcatctttt
     2221 ggctgatgct cgtaaaaatc aataacggac tggatcatat caaatgtttg atgaataagc
     2281 atcgggatat actttgtgaa tgccagatag ataaggtaaa cgatcagtgt ataaatcatg
     2341 atagctaaaa cacctgtagg gattcggaca aatcgctgaa tgatttgaac aagatgaatc
     2401 atcaaataag taaagataaa tgtgagcaaa atggttgtaa tcatacttct aactaagaaa
     2461 agaacaaaaa cgacaaataa taaaacagtg agacggcgta ttttttcatt cgccaaaaaa
     2521 cgctgataaa cggtcaaatc gacaacctcc aaataacaaa aaataaacac gttattcatt
     2581 atatgatgga atatgaaaat aagaaagaga aagagcaaaa agaaaaaccg ctaaatgacg
     2641 ttatcttatg agttggtttt cctcataaaa tcgttcatct agcagtttca gccctttttc
     2701 tggtcttgat gaaaattatc tagcattttt cgggtcagat gttgcagtgt atccaattct
     2761 tcctgttcaa gtggaagata agacttgatc ttttgtggaa taccttctgc ttgtttttgc
     2821 aacgcagcac cttgaggagt cagcgtaaca tttacgattc tttcatcacc cgtttcacgc
     2881 tgacgattaa tataattttt ttctgctaac ttttttagta agggagtcag cgtaccagaa
     2941 tccagacaca aatcatttcc tagctctttc actgaagtct ttttcttttc ccataaaact
     3001 aacatcacaa gatactgggt ataggtaaga ttcagcggct tgaggaggga agtgtagtgt
     3061 ctaatgattt ccttagaggc ggcatataaa ggaaaacata cttgattaac taaatataga
     3121 tcttccattt ttccacctcc gctgttttac tatagtgtat catagcggat ttacacaaaa
     3181 aagtctattc actaactgct ttcagagtaa cttctatatt tccagctgta gcttttgaat
     3241 acggacacac tttatgcgct gcctctaata attcttgcgt ttcttccaga gaaagacctt
     3301 cgatagatcc ttcaatctct acacctaaga caaagctcgt ttcactttct gaataaaggg
     3361 aaacttgtgc actgacagta gaagccccag agatattttt ttgctgtttg atcaattcta
     3421 atgcactgtt aaaacatgca ctgaaaccag ctgcaaacaa ttgttcaggg ttggttgcat
     3481 tttcgacgcg tttaccagga gctgcaatat tcaattcgaa tgtacgatcg ggattatgaa
     3541 cttctccaga gcgtccgcct gtgttgatca ttttcgtaga atacattttt ttcataaaat
     3601 taccaccttt aattatattt tatacaattg aattttacac aatataattg tggaaagcaa
     3661 agaaatggat tggcataaga taaataagag aaagcacatg aaacagacgc gctttctcga
     3721 atagcaaaca tatacaaaaa aatatcagag acaaaaacta atctcatcat aaacattaca
     3781 tatatttctc aaaaagttgt tttctttctt caaaacttac ttcacgatgg acctgatgaa
     3841 gcatccataa ataaccaaaa ggatctctga acattgagtt agtcacacca aagtccatca
     3901 tttctgtcat ttcttgaatc tctacacagc catttcgtaa tgctttatta tgtgtgtcct
     3961 cgatatcaga gacgatgata ttgaaccaga tcgtatttgg atgttcagca gtaggagcag
     4021 tcaaaccaag agacgtgttt tcatccagca tatggaatct cgtgccatac aaggtaaaaa
     4081 tgacttcatt attgcctcta gggtaatctg ttgcctcaat cagttgtatt tcatcaaaga
     4141 ttgtttgata cagttctagt gctttcaaac tatcatttaa tacaaaatct atttccgttc
     4201 ctttatgcat aaagatctcc tctccttttc agtcgaacag ttactagatt cttttattat
     4261 accagttaaa gaaaacgcat tcaaaaaaat agcggattta cttaccaact cattcgattg
     4321 gatgataagt aaatccgcgt cccattactt ctgctacgct gctgacaatg acaaaggcac
     4381 ttggatcgat ttcttctatc aatggtttca ttcgggaaaa ttcatgatca gtcacaacga
     4441 tcaataaaac ctcttttgac tttcgattat acccgccttt tgcctctagc agggtaaggc
     4501 cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc caaataatgt tcactcatga
     4561 ccataacaga tttctttcga ttgatacctg tttcgatata agtcatgaca attgaagtga
     4621 ttgcaataga taaaatagca taaagaaact cttctattcc aaaaacaaac agattaagag
     4681 aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt attcaagtgg aaatattttt
     4741 taaaaatgag gggagggatc gtcgtaccgc cgctagaaga gttgttttta tataaaatcg
     4801 caactcctaa agcaaaaatc gcacttccaa aaatgacaga caacaaccga tctgaagtaa
     4861 tcattgtttc tggaactatt gcaatgatta aaggaaaaaa caagcttccg aaaagtactt
     4921 taaaaaaggg ttttttacct aaaaatagaa gagctagtac caacataacg atattcaaca
     4981 ctaaaataac tatagctcgg tcaaatccta aaacagcttc cagtaatatt cctagaccgc
     5041 tgacacctcc agcagcaata tgatgaggcc ctaaaaacat attgatagcg actcctaata
     5101 tgaagagggc aaaaataatc ttaatgtatg tccatataaa ttgtctcgtt tctttcattt
     5161 atccacttcc ttaattcatt aataatactc ttatcttatt ataacttact tttcacctta
     5221 agttaacttt aattaataaa aaaattaggc tagtctaaat tattatgttt cattcttcta
     5281 gaatatactt ctacgttaaa aactgttaaa aaacttttac tttagaaaat tggagaaatt
     5341 gatcgcttaa gataaattca ggacactaaa tagtacagga agtagattaa aacttttaaa
     5401 agttttaagt ctatctattt ggcaagttta aaaaataaaa attcggtatc aaaaaactgt
     5461 taccgataac atttttgaaa gcgttgtctt ttgtttctct atcacttata gtgatgacgt
     5521 catattaaaa cacaaggagg aaatcaatgg atacagctgc aatttatcat cgaccggaaa
     5581 gtgaatatgc atttctatat acgaatacaa gatttcgtat tcggcttcgt actaggaaaa
     5641 atgatatcaa aaaagtatat gtgctttgtg gagatccata taccataaca actgaaaaat
     5701 ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac caatgttcat gattattggg
     5761 aaattgaagt gacgagcgaa acaaggcgct tacagtatgc gttccatgtt gttggggaag
     5821 ataacacgga ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta
     5881 ctgaaccaaa tttttatttt cgaatacctt attttcatca aatcgatcga ttcactattc
     5941 cagaatgggt aaaagaaaca gtatggtatc aaatttttcc cgaaagattt gcaaacgggg
     6001 acaaagcaaa tgaccccgaa cacactttac cttggggaag taaagatcca gaccgagaag
     6061 acttcttcgg gggagatttg caaggtgtga gggatcattt ggattacttg acggaccttg
     6121 gcgtcaatgg catctatttt tgtcccatat ttaaagccac atccaatcat aaatacgata
     6181 cgattgatta ttatgaggtg gatcccgctt ttggcgataa aaaactttta aaaaacttga
     6241 ttgatgaagc acataaaaga gggatacgta tcatgttcga tgctgtattc aatcatatgg
     6301 gtgtccattc accacagtgg caagacgttc tcaaaaacgg agaaaaatcg atctataaag
     6361 actggttcca catccgtttt ttcccagtag attcttatca gatgactgac ttaccagaaa
     6421 ctgctgaaaa tatcccatat gatacttttg cttttactcc attcatgccg aaactaaata
     6481 cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc aacttattgg ataaaagaat
     6541 tcgatattga tggatggcgt cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa
     6601 aattcagaga agcagttaca gaaatcaaac cagacatcta tatccttgga gagatttggc
     6661 attcctctca agcttggctg caaggggatg aattccatgc agtgatgaat tatgcattca
     6721 cagattcgat taaagattat tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg
     6781 gaatgaatca tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca ttcaacttat
     6841 tagattctca tgatactgcc aggatcttga ccctttgcca aggtaacaaa gaattgatga
     6901 agtctgtgat ggcctttatg tttcttcaaa aagggtctcc ttgtatctat tacgggacag
     6961 aaatagggat gactggtgaa gatgacccgg attgccgcaa atgtatgata tgggagaagg
     7021 aggaacaaga tctagagcta ttcggtttta taaaagaact tgtttctcta aggaaacagt
     7081 tgtctaagat tatttctgaa ggttctactc agtggctaat agtatatgat cgtgaagata
     7141 agctctattt cacacgggaa ctagagggac agatcatcta tgtttacttt aatcagtcaa
     7201 aagaaccttg ggtagtagaa caggaaaatg aggtgattct tagtcaaaat tgtcagttgt
     7261 tagaagacgg gaaagcagag atacgacaat acggattttt gatcatgtgt tgaaaactgt
     7321 taccggtaac aacaagaaag cgtttgcaaa tgaagatttg tgcccattat aataagggtg
     7381 taacagcacg ttgaatacac aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt
     7441 gggtttaggg ttaatctctt taggaatctt aggcggatta gcggcttgtg gcaatggtaa
     7501 ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta acagtttccg ttgattctgg
     7561 atataaagat tatataaacg aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat
     7621 caagctagta gaaaaggaca tgttcgatca gttggaatca ttagcgttag atgggcctgc
     7681 tggaaaggga cctgacgtta tgatggcagc atatgaccgg atcggtgcgt taggacagca
     7741 aggacatctg gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa cagacaaagc
     7801 ccaagtgact tatgatggaa aaatctacgg agaacctgca gtaatcgaga cccttgtttt
     7861 gtactataac aaagatttag tcgatacagc accatcaacg tttaaagatt tggagaatct
     7921 atccaaagac agtcgttttg cttttgagtc agaagcagga aaaaatacag gatttttagc
     7981 taaatggact gacttttatt attcctatgg tttaattgca ggttacggtg gctatgtatt
     8041 tggtgatgat ggcacaaatc cttcagatat cggtttgaat aatgcaggtg ctgtagaagg
     8101 aatctcttat gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc aagacataaa
     8161 aagtgcagga gacttcgcca gccagcaatt catgtccaat aaaactgcag caatcattga
     8221 cggtccatgg caagcgcaaa catacaaaga aaacaatatc aattatggtg tagccaaaat
     8281 tcctactcta aataatggac agccttatca accgtttggc ggaggaaaag gatgggtagt
     8341 cagcaattat gctaaaaaca aagacctctc tcaaaaatgg cttgattatg taacgaatca
     8401 ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag
     8461 ggaaacagca aaggctaaaa acgatgaatt aacaacagct gtgattgaac aatatgaaac
     8521 agctcaagcg atgccaaaca tcccagaaat gggcgaagtt tggacaggtg cagaaaactt
     8581 gatgttcgat gcagcatcag gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa
     8641 aacaatcagt gaagccatag aacaaaaata ctgattaaaa tgataaccgt ccatttttat
     8701 aaaaaatgga acaggtgctt cagataaaag tggcaagagc gagatggaaa attatcttgc
     8761 tcttgttgcc attatcttaa aagcagtttg cggtttagca agtttagagg gggcattcag
     8821 atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt
     8881 ccagggatgg ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt tttcgccata
     8941 ttcgtcctat tcattggtca gatgcttctg ggcggatttc atgcattggt aggattggtt
     9001 actttgggaa gtataccaat ggaagatcat tcattattta ttttgattga aggcactttg
     9061 caattcatca ttacaatcat tttcttgatt ttttacggtt tgaatctttt agacgcttat
     9121 cgagtagcga agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata
     9181 cataatatag gtgatgaagg ttttccttat ctattgacac ttcccgctta tcttctcatg
     9241 atttttacga tcattttccc agtcctagtt acattgttca ctgcatttac taattatgac
     9301 ttcaatcata ttccaccagc cagtttaatt gattgggtag gggctaaaaa cttttttagt
     9361 atcttctttt taagctcata tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt
     9421 tggactttat gtgcaacaac tttgcaaatc acccttggta ttctcacagc ggttgttgct
     9481 caccagaaat ttattaaatt caaacgtatt ttcggtatca ttttcttatt accatgggcg
     9541 gtcccagctt ttattactat catgagtttt tcgaatatat tcaacgatag cgtaggtgcc
     9601 atcaatactc aagtgattcc tttcataaac cagcttcctt ttgtagatat cagtgcaatc
     9661 tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta tcttagtgca gggctggtta
     9721 ggatttccct atatctatgt gatggttaca ggaatcttac aatcgatttc agaagactta
     9781 tatgaagcag caaaaatcga cggagcaaat gcatggcagc gcttttcaaa catcacgctt
     9841 cctgctgtct tcctagtggc agcgccaaca tttattacac agtatacggg gaatttcaat
     9901 aatttctcca tgatctatct gtttaacaat ggtggtcccg gaagcgtagg cggaggagct
     9961 ggttctactg atattcttat ttcctggatc tataaactta cgactggaac ttctccgcag
    10021 tattctatgg cttcagcaat cacgttgatt atctccgtca tcgttatctg tgtgtcattg
    10081 ctggtattta aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa aaatttcatt
    10141 cgagtgctcg attccaaaaa cgactttctc acttttttac ttactttttt cttttaatac
    10201 tgtcagtcat tatcatctat ccgcttttga tcacagcaag tactgccttc aagtcaggaa
    10261 atattgcagc ttttagttta gatctttcaa gtgactggac tttaggaaac tttactcggc
    10321 tattcagtga aacactttac gggacttggt ataaaaatac attgatcatt tctgtcctaa
    10381 caatgatcgt acaagtgact gtagtaactt tggcgggcta tacttatagt cgctatcgtt
    10441 ttatcggccg caaatcaagt ttgatgttct ttttagtgat tcagatggta ccgactatgg
    10501 cagcattgac tgccttttat gtcatggctt tcttattaga agcgctcgat aagtactggt
    10561 tcttgacaat gatctatata ggcggtggaa ttccaatgaa tacttggctg atgaaaggat
    10621 actttgacac tgtaccaata gatttagatg aatctgctaa acttgatggg gcagggcatt
    10681 tcaggatttt tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat
    10741 tatgggcttt tatgggacct tttggggatt tcatgttagc taagttcctg cttcgaacac
    10801 ctgaaaatca gactgttgct gtcggcttac aaacatttat ttctgatgtg acaaaccaaa
    10861 gagtctctct ttttgctgct ggggcaattt tgattgcact accgatttgt ctattattct
    10921 tctttttaca aaagaacttt gttaccggcc tgacatctgg cggaacaaaa ggataggagc
    10981 agagtatcat gcaaacattt ccgttaaact atttttccgc attacgtacg ccaactcaag
    11041 tgtttgcggg acgcaaacta ttatcatggc ctaaattttt cttgatattt gtatttttag
    11101 tttccttgat ggtcatgcct gtgactcttt tttatgccaa tcagatccaa gcgattccat
    11161 tagaacaatt tctatccgtt cattcgctga tcgatgaaca aggaacccaa aaattttctg
    11221 aacttgaatt atctgaaact gggttacaag catcgcaaca aacgatagct gtgacccctg
    11281 aaattttggt aggtgtgtct ttatctgaga aacaacaatc cgatcatgga acttttattg
    11341 attttgagaa ggaacaatgg gtgatccaac aaaaggataa atctggtata cgccgctata
    11401 cgatgaatta tagcccatct ttccaaccag attctgtacg tacgcccgaa gatttccaac
    11461 gatttttaga aagagaattt tatgccagta atcgtccaac aatcatttta tcttatagtt
    11521 taagtcttgg tcttgtccta tttgtcatga cgagtctcat tttattcggg gcttcctttt
    11581 tcttgtggat gacaagaaaa agccaacttt catccattca tacattcaaa gagagtgcaa
    11641 atttgatgct gaatgttatg ggaataggtt ccatggcagc agcagttgta gggtttatcc
    11701 actttgactt tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat
    11761 gggtttttgt caaaacaaga ttcaaagata agcgtgtcga atgacaagtt taagcgattt
    11821 atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa tttctgaaaa
    11881 aattaaactt cttagttgat tcttttcttt tttagtatta acgtaatagg gtagtttttt
    11941 taaggaggaa aatcatgtta gagaaaactt tatacaatca attattttca cgttctttct
    12001 cgctccctgt cgaagtaaat tattgggacg gtacaacgaa acaatacgga gatacggaca
    12061 ctcctcccca aatcaaaatc aatatccatg aagaaatccc aatgaaagag attacaagta
    12121 acgcttcttt ggcactagga gaagcctata tggatcatcg aatcgagatc gagggaagta
    12181 tccaagcatt gatcaacgat gcttatcagc aagcaaacag ctttttacga ggaaaagatt
    12241 atctaaaatg gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa gaagatatcc
    12301 atgcgcatta tgacttaggt aacgactttt ataaactttg gctagatcca actttgactt
    12361 attcttgtgc ttatttcaat acacctgaag atacgttgga acaagcacaa atcaataaag
    12421 tccatcatat cttagacaaa ctctttatca aagaaggcga aacgttgctt gatatcggtt
    12481 gtggctgggg aacattgatg ttcactgcta ccaaagagta taatgtcaag gcaactggga
    12541 tcacactaag tgaagaacaa tacgatttga ttaccaaaag aataaaagaa gaacatttag
    12601 aagataaatg ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa acgtttgatc
    12661 atatcacaag tgtaggtatg ttcgaacatg taggttctga aaatcttgaa ggttatttca
    12721 aagtggtaaa agatttattg aaaccaaaag gaacagcttt gatccatgga atcagccgac
    12781 aacaaggcgg tgcgacaaat gcttggatca ataaatatat tttccctggc ggttatgtcc
    12841 ctggtgttac tgaattagtt gataatatta caaaaaacga tttgcaactg attgatttgg
    12901 aaagcttacg tcgtgactac caattgacat tagaacattg gacaaagaat tttcatcaag
    12961 tccaagatca agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg gatctatatt
    13021 tacaagcttg tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa tatttgcttg
    13081 ttcaacctgg aaacaatgac atcccaatgc atcgtgcata attgaggcaa aagtactgtt
    13141 taaagactat ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac ttttttgtct
    13201 aacttctagg attgtctcta aagcttagtt tctttttctt tatattttca taataaaagt
    13261 ttggttcttt aatttttaaa catgacagaa aattttttct tcgttatact tgtaatagaa
    13321 tactatttta taacaaaggg gatcgaatca atgaaaaaaa ctgttattta tactagttta
    13381 tttgcagctt tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac atctgggaat
    13441 acgtcttcca gtagcgaaca gcgctcttca caggtatcgg actcctcaac aaaagcaagc
    13501 acatcttcgt ctagccagac aaaaagttct agtgaagcaa gtactcagtc agatatcaaa
    13561 gtgtctgcag ctcgtgcgat cgaattgttc caagataaat acccagatac tgcaattact
    13621 agtttggaat tagatagtga ttggggatct tacttctaca agatagaagg tgtcgatgat
    13681 caaaaagaat acgaagcaag aattgatgct caaaatgaaa aaatggagac agagaatcca
    13741 gaaactctag atcgagatga acaaaatggt caaaagaaaa aagaagatgg tctggatgtt
    13801 gataaattga tcacgattga ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt
    13861 acggcaacag attgggattt ggataaagat ttaggtacta cttattggga agtcaaagta
    13921 aaagacggca atcaaagtac atcagtcaag atcaatagcc aaacaggcga agtactgtca
    13981 actgaacaag atgattaaga atttcttcgg aaaacaagag ttgatttttc ttttagtcat
    14041 gatacactag acaaaataac ttgtttgaag gagcgattta cttgagttta gaaacgacct
    14101 ttgccaatca tatctttgcc aatcctttga tgaatgcatc tggtgtccac tgcatgacta
    14161 cccaggaatt agatgaactg gctcattcag aagcaggagc atttatcact aagagctgta
    14221 cgatcaatga acgaaaagga aatcccgaac cacgctattt tgatgttcct ttagggagta
    14281 tcaattctat gggacttcca aacttaggtt tttcttatta tctagagtat gctcttgcgt
    14341 atgaaaaagt gcaggaaaac caaaatcaac cgttattttt ttccattgcc gggatgagtg
    14401 tccaggaaaa cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat gggatcactg
    14461 aattaaattt atcttgtccc aatgtgcctg gaaaaccaca actagcttac gattttgagg
    14521 ccacttatga aacactgaaa gaagtattca gcatcttttc taagccgcta ggaatcaaat
    14581 tgcctcctta ttttgatttt gctcattttg atcagatggc tgatatcttg aatcagtttc
    14641 cattgaccta tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc gatacagaac
    14701 aagaagcagt agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga gagtatatca
    14761 agccgactgc tttggcgaat gttcgcgctt tttatacacg tttgaaacca gaaatccaaa
    14821 tcattggtac aggtgggatt cggacaggac aagatgcatt tgaacatctg ctatgtggcg
    14881 caagcatgtt acagattggt acagagcttc ataaagaagg gccggaaatt ttttctagaa
    14941 tcataaaaga attaacgcag attatgtcag aaaaaggata tacgtcaatt gacgaattta
    15001 aaggcaaatt acgcaccatt tcatagcaaa gagacgaagg tcatgattga tcttcgtctt
    15061 tttttataaa aaacactcgt ctgttcattc ctgtgcaagc gtactcattg tatgttagac
    15121 gaacttgtgg gaaaataaca gaggcaaaaa ggaggataat atgaatgatg caacagaatt
    15181 agcaaaagag cttaaagaaa aagaatttag ttttcaagaa ctagttacgg atatacagaa
    15241 aaaaataaag aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag
    15301 taattcccac gagctattga ttccccagtc agaaaaaggg ccattggctg gtatcccatt
    15361 tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta gctacttcag gatctcgttt
    15421 atttgaaaca caccgagctt ctcatacatc taattatgtg cggcaagcag aagcgatagg
    15481 tctagttcct tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc
    15541 agtcatctat ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag
    15601 tggaggagct gctgctgcag tttcatcagg aatcgttcct ttagctggtg ctagtgatgg
    15661 cggtggttct attagaatac ccgcttcgtt ttgcggattg attgggctga aaccttcgcg
    15721 agggacgatg ccagttggtc catatgcttg gagaggatgg caaggagctg ctattgattt
    15781 cggcttgaca gtttctatga gagacacgga ggctttattt tatgggatga gaagtatcca
    15841 ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg
    15901 gttgaagata gctttttgtg ttgattcacc gatccactca cagattagtg aagaggcagt
    15961 acttgctgta aaaaaagctg tagatttcct tggaaaagaa gggcatgatt tgacagagat
    16021 tccttaccca ctagatggtc gtaaactgat cacgtcttat tatcagatga atggcgcaga
    16081 aacagcagca atgatccgct cgattgaaac aggcatccag cgagctgtca gaaaagaaga
    16141 acttgaaccc atttcttgga ctttgttcca atatggtcag aagctctctg ccgctactta
    16201 catcgattct ctgcatgtat gggatgaagc agcaattgtc atggaaaacc tacatcaaga
    16261 atacgatcta ttcctttcac caacgactgc atttaccgcc cctaaaatca gcgaagattt
    16321 gcaaagtgat gcgataagag cacagatgga acacatcagt gattgttctg aagtagaagg
    16381 tttagacgtt gtttcttcca tgtttgagga gagcttgaag atcacacctt atacacagtt
    16441 ggctaatctg actggacaac cagcaatcag cttgcctact catttgacaa aagaaaacat
    16501 gcctctagga atccaattta tggctgccag aggaagagaa gatttgctat ttcaaatcgg
    16561 ctatctattt gaagcaaatc atcagttcca tttaccaaat ccatggaaat agcagaaaca
    16621 aggagaagaa gaatgaagaa aaaaagattg tttgctctaa taactagtgc cttttttatt
    16681 ttaagcgctt gcggtacagc tgccgctaat caggaagata acagctggag taatgtagaa
    16741 aaaaagaaag aattagtagt agcgacttct ggcacactgt ttcccgcatc ttattataat
    16801 gatcaaaatg aattagttgg ttatgatgta gatttggcaa aagaggtggc taaacggtta
    16861 aatgtcaaaa tctcctttaa agaatacaac gtggatggac aaatcacttc tgtttcaaaa
    16921 agagaatcag attttgctgc gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc
    16981 tccctttcag agactttcaa atattcattt gacagtatga tcgtacgaaa aaaagatgat
    17041 tcaggaatcc actcacttga agatcttaaa gggaaaaaag cagctggcga acccaatact
    17101 agctatatgc gtttagcgga aaaacacgga gctaaattag tcacatatga caatgccacg
    17161 aatgatcagt atttaagcga tgtagcaaat ggtcgaacag atgttattct aaatgattat
    17221 tatctgcaga aaatggccgt atctgcttta ccagatatcc ctgtaaaaat actggagaac
    17281 gtttatttca atgaaaacga aagtggtttt ttattgaaag aaggaaatga tgctctgaaa
    17341 aagaaaatcg atggcgtatt aaaagagatg aaagaagatg ggacgacaaa gaaaatatct
    17401 gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg
    17461 agtgagtgat gtactttccc ctaattgatt tttccctcat gtggacttca cttcctttcg
    17521 tactgcaagg cttggtatac acattaggga ttggcttcgt gtcgtttctt ttaggaaatt
    17581 tggtagggtt gcttttgaca gttttaggtc ttttggattg gctgcctctg aatgtattta
    17641 ttcgttttta tttatcgttc tttagaggca ttcccgcact agtcctgctg tttctgttat
    17701 atttcgggtt accttatcaa ttaagtgcct taacggcttc agtcatctgc tttacgatta
    17761 ccagcagtgc atttattggt gaaatctatc gaggatcact agcaggcgtt tcttcaggtc
    17821 agtgggacgc tggtttagct tctggttttt ctttctttgg ggtattgaaa tatatcgttt
    17881 tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg gatctagtga
    17941 aaggtacttc gttagcagca acaatcacag tgccagaaat atttcaaaaa gcaaagatca
    18001 taggcggtcg gacattcgat tatttttctc tctatatttt agttgcactg atttattggg
    18061 tcatttgtat cgggatcgaa aaagtgcaac agtatttaga aaaacgttct atccatcagt
    18121 caatctgaaa aatagaaatg gtgctagcgt ggctggcacc atttctattt tttgtttctt
    18181 gtatattctg cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc
    18241 ggaggtagaa aacatgaaga ccccgctaac catcgaaata gaatcctcgt tataccaata
    18301 ttgcatcgag caaggcggct tggttgtaga agaagtcacg atgcctgatg accaaggaat
    18361 tgtagatacg cttagctgct tgacaaaatt tgatggaagc cgagaatggc gatgctatga
    18421 gctgaaagtg acaaaagcag attttcgctc tacagccaaa ctgtcatttg ttggacatta
    18481 taattatttt gttttaccaa gtaaactata tgaagaagta aaaactgaaa tccctacagg
    18541 aataggtgtt ttagtgtatc gtccgtacac ggttgaagag gagatgcctg cctctggtac
    18601 atttctgatt gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag aagctttgac
    18661 aaaccgcttc atggcttctt tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg
    18721 cgtaaaatat tttccaaaag atcaattgta caaagaattg aaaagacgtt cagaaaatca
    18781 agatccattt tctaacgaac gatattatga acggttttta gaggaaatag ctagtcaggc
    18841 aatcaatgag ttgcaagaag aagttatcgc gcttcagcat gattatgaat atttgaggca
    18901 acaacagcag atccgtcggt tgcctacaga gccattggag tgaaaaatat gtactatcct
    18961 tatttaagag gaaaacaatt tgacctatta gcattaaaag aagcactgtc gcgaggattg
    19021 ctctccaata aaatccaacc agtgatcgaa ccagtacgtg attccgctac gcttaaaaat
    19081 gtgatcgaat tatttcaaaa aaagcaccat ccaatcgctg tgatccaaaa tccccaagta
    19141 ggacaattca aattattcga tcagcatgtg cacagttgga cagtcaaaga aaatagctct
    19201 gtcgtccctg cacagatctt aacgccagaa aatctcagtg aagtactgga ttcacctcca
    19261 gcgtttcttg ttttcgatgg acagcactac ccaaaagata cagaggtgtg gaaacagttg
    19321 gcaggagttg attcgaaatt tttgatacca gatactagtc gctttcgcat ctggctgccc
    19381 gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa agcatgttga aagttatgca
    19441 gataaaacag atgacttttt tagtgatgac tatctttttt tccacgcaga cggatatatt
    19501 ggattttctg atttcacgat cgaaggtagc cgttatttcg ataaagggtt ccctagccga
    19561 gcgcttgcta tccatctaac gtatattgat gcttacggca atatacgcgt gaaacatttt
    19621 gtctctgaca gtaacgactc tgctaaagac caagcattga agtttcttga agcaggagat
    19681 aaaatgaaaa aatggatcat gcgtcaccat catcaactct tgattacttc aggaatgatt
    19741 gaattattgg ctctttacca gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg
    19801 tcacttatgc atcatttgga acttattagt cacctgctag atcatccaaa agattggctt
    19861 aaatattatt caaaagttca ggaactatat gaagtgatcc aatgattaga taatgtaaat
    19921 gaaaaaggaa tagaaaagag gaaactggat gtatattcgg caaacaagga aagaagaagt
    19981 agaagaagtg gtagaaatca ttaatgccgc agcggatttc attgcttcgc aaggctctcc
    20041 tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa
    20101 agaaagctat gtattaatag tggaagatca aattgctgga acagctgcat tagtctcagg
    20161 cgtggatccc gtttacacaa aaatagatgg tagttggaaa gaatcaactg gtccttattt
    20221 atcgattcat cgtgtcgctt tagcagaaag tactcgagga aaaaaactag caaaaccgtt
    20281 cttagaacat ttgttgacga ttgcagccag caaaaatatc caagatatcc gtatcgatac
    20341 ttatccagct aatgaaccga tggaaaagac catctattct gctggattca cttatcaagg
    20401 gatgattgaa tttccttttg aacacggcga aagaaaagca tatcaaataa tcatcaattg
    20461 actataaaat catctgagca aagataaaga cattaacact actgaaaaaa acaatcatat
    20521 gatagccaag aggttgtaac agaagtggcc agcttcaaaa aacaagaaag aatttccgaa
    20581 aatgcttctc aaatttttgt ggaattttag cgtattttac gaaatagctg ctttctgaaa
    20641 ctcccttcat cgagaggtcg tgacatagct tttgtcacga tctctcggct tatcctacga
    20701 gtattattcg atacatttat atcaataatc ctttttttat tttatctgca gcttcttgga
    20761 acgctccatc gtttgacacg actaaaagtg aggggatagc tttcaaacgt ttcaactgtg
    20821 ctaaatcttc tacataaacc gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt
    20881 ttttcaggcg cgattgcaat gtttctttag agatcgtaat aaagatagga cggactaccg
    20941 tcccgaaata ctcatataaa tgccaaaaac catttgctgt caaaacggcg taacaatttc
    21001 ctactgtcag tttcttttta atttctgctt tactggaacc atatttatgt tgagcatatt
    21061 ggtcccactc tgcaaactca tttcccttga tcatctgatt gaatgtattg tcagaaacaa
    21121 aataataatc ctcttgatct ttttccccag gtcttggtga tcgattcgtg taagtaatga
    21181 tcttttgttc cggctgaaaa atagcttctg cgagtgaagt ttttccagaa ccagaaggac
    21241 cgataaatac atagcaataa ttcattgtca tttctccttt gatgattgaa caagtgaaaa
    21301 agctttggaa aatatttcca aagcttttaa aagtaacttt gcttttttgt attttatacc
    21361 agcaagccag tcacaatagc acttaaaaag ctgactaaag tcgctgttag caaaattttc
    21421 aatccgtgtt ttgcgacaag atttcctttt tcttcattta atcccttcat cgcaccagaa
    21481 atgatcccga tagaagagaa gttggcaaat gaaacgagga agacagatat aattgctgtt
    21541 gtacgctcag taaaatggaa attaccattt gctaattctg tcatagcaac aaattcattt
    21601 gtaaccagtt tagtcgccat gatactaccg gcatccactg cttctttcca tgggattccg
    21661 ctgatgaagg caagaggggc aaagatgtaa cctaggatct cttggaaagt gatgccgaat
    21721 atcccgtgga agatagcatt gatcatggca atcaaagcaa caaaaccgat aagcatagca
    21781 gcaaccgtga tcgccacatg gaaaccatca agaatgtatt ctcccaacat ttggaaaaat
    21841 gtctgttctt tgttttcttc aatgatcagc tcatcttctt tttcttctaa tacataggga
    21901 tttacaattg aagccagaat ataaccgcca aataagttca aaacaagggc agtgatcaca
    21961 tatttagaat cgatcagtgc catataagat ccgacgatcg acatagaaac tgtagacata
    22021 gccgatacac tcatggtgaa caaacgtttc tcagatagga agggtaattc cttttttatg
    22081 gaaataaaaa cttccgattg tcctaaaata gcagaagcta ctccattata agattccaat
    22141 ttgcccatcc cgttgatttt acttaatcct agaccaacag ctttcataaa taatggaaga
    22201 atcttgatat agcgtagaat accgataata gcagaaataa atacaattgg catcaatacg
    22261 gaaagaaaga aaggtgcact tcctttattt gctacaccgg caaaaacaaa gtcaacacct
    22321 tgcccagcaa aagcaagaag atgatcaaac atttttgcca atacagaaac aaccgctgta
    22381 ccaaaagttg tacgtaataa tacgaaacca agaacaagct gtaaaacgaa catcacaatt
    22441 agtggtttca atttgattgc ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg
    22501 acaaataaac caataatacc taataaataa ctcaaataat cactcctatt ccaaattccg
    22561 ttttatttta cctgttttgc aaataaaata ctaatcttta gtgaaatttt cattactttt
    22621 ttagagtgaa acgcattcat tttccattag aaaataaagc gaacaaaata aaaggtttaa
    22681 aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac tatcgattaa gctaatagtt
    22741 tcgttttcaa catttataag taaacttttt ctattggtaa tatagatttt cggaagggta
    22801 gacaggtgcg caggtaatat cgattcctag ttttttcaat gtttgttcat catttttatt
    22861 gaccatcaca gtagagtggg cttgtgctcc ctcaagttca gctagtttat cataagcaac
    22921 ttgagcgatt ggattcgtta ctgcactgat tgataaagcg atcaagactt cattgatgct
    22981 tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat ccttgtattg tttcaagaac
    23041 taatggtgac aataaaggaa tgtcatctgc gatatgggct aaatgtttta ctgcatttaa
    23101 gataactgcc gctgacgcat ccatcaatga acttgtacgc ccggtaatga tgacttcatc
    23161 tggtaattcg attgcaatca cagcagaagg atcttttgta ggactttgcg cttgaatctt
    23221 atctgcatag tcatgggcag ggactaccac gtttcggtct tcaggacgta aaccgacttc
    23281 ttccataatc agtttgattc gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc
    23341 gcattctgtt tggaaataac gacggatgat ttcttgttta gaggcttctt ggacaacggt
    23401 atcatccacg atcccaaatc ctacacgatt cacacccatg tcagtaggag attgataaac
    23461 agattcttta ccagtaattt tttcaataat acgtttgatt acaggaaatg tctcaacatc
    23521 ccgattatag ttcacagcta ctttattgta ggcatcaaag tggaaggaat cgatcatatt
    23581 gacatctttt agatcgactg tggctgcttc ataagcaatg ttcagcggat gtttcaacgg
    23641 aacattccat actgggaaag tttcgaactt ggagtaacca gctgcatgtc ctttttggct
    23701 ttcatgatac agttggttca agcaagtagc gagctttcca cttcctgggc caggagctgt
    23761 cactacaacg attggtttag ttgtttcaat atactcattt tttccaaaac catcttctga
    23821 gacgattttt tctacgttga ctggatagtc ttcgatttct gagtgtttat agaccttgat
    23881 gtttcgtctt tctagtttat tgataaagac tttcgtggca ggctgtccac tatagcgagt
    23941 gataacgaca ctgttgatta ataagccata accacgtaat tcatcgatta accgcaagat
    24001 atccatatca taagtaatcc cataatcacc acggatctta ttacgttcaa tatctccagc
    24061 atagacgcaa atcagtattt cagcttgatc tttcaatttt tgcagcaatt tgattttcgc
    24121 atcttcttca aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta acttcccacc
    24181 aaattctaaa tataatttgt catagtggtt cactcgttcc aaaatatacg ctgactgctc
    24241 ttcaatatat ttttctgaat caaaaccaat ttttttcact aggttccctc cttaagtact
    24301 aaagcatttt ttcatttttt tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa
    24361 acaaagaaaa tggtttaaac caacttgtct agatagttat tcattggtat gatagttttt
    24421 gataaggaag gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat aaacactata
    24481 tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg gaacaattga
    24541 cgtttactta tctaaacgaa gaaacgcagc taactcgtga tgagcatcag cgcattcatt
    24601 tcacaacaag agatctgcct agtaaataaa aaataagata aaggtcatcg gattcgattc
    24661 tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca
    24721 gcctgtttta tggagcattg cccgctcttt tttataacaa ttttttacag aaaatggtac
    24781 tattgaagta ttaataagtg aaaaacagat gagtaagagg aggcaagtag tatgaaagag
    24841 ccagatacaa atgcaaatta tgagccagac gaactagagg agacgaacgc ggatcaggat
    24901 acaggacaag aatcgtcttc tttttacgat atcaaagaaa aagcaagcat gaatctttcc
    24961 caattattag aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag
    25021 atctaccgtt tatacaatgg ccgattgaat gaagaattaa aaagaagctc aaatgaaaat
    25081 cacgagatcg atcaattgct gatgcgtaag atccatgatc gttttttaca ggaatttcct
    25141 tttatggaac aagtagaaaa catctcgcca acgatgagct attacaaaat cggcacttat
    25201 taccatgatc ggcccacggt cggtattgat gcaagtctgc cagaaatttt tgttttgcct
    25261 aagatcgatg aagagtggga gaaatattcc caaccggagt caacggacta tgacaaaaaa
    25321 atcaatgagc tagacgcaaa ggtgatcact gctcagacag aaattgaacg tttagatgaa
    25381 caaataaaag aaatcaatcg acaaatgact gatttgaacg acaataaagg attcttgaat
    25441 cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga agaagcaagt actatctaat
    25501 gaaaaattag gctggcttcc ttatattgag acgcctgaaa caatccagca gcaaaaagaa
    25561 gcattgaagc aagaagcaag agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa
    25621 aggcaaatca gacgctattt cggtagcaaa gagggattcg gtcaagcaat ccatgaattc
    25681 ttgatgaatt atcttggcaa tgagaatcct aataatcaat ctaatgaagg aggttccgag
    25741 tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa gaacaaacag aagatcatga
    25801 agaaacgaac acttcattgg aagaacaaac aagtaatcct ctggatgaca cagaagaaat
    25861 ccctatgcta gaggaaacaa aaatacatga aaaagaaaaa gtaactccga cgaatacgaa
    25921 tactgcagaa aacacccatg cttacgaaat aactgaaaat cccgggtatt tagaaggaaa
    25981 agagcaagat catcaagata tcgtcagttt gattgaaaca gcggaagcag aattagcgga
    26041 tttacgtatg agaaaagatt tgatcgaatc agattttcca caattattag aatcatatga
    26101 tcgttttata ttgaatgaag aaccaatctc cacgatcgga aagacaagcc tgatcgaata
    26161 cttcacgtat gaattactga aaccgttgaa tgatattggt ctggaacggt caaattcagc
    26221 ttatgacaca tggaaaacaa gacatttttc gatcaattat acgttgaatg gacaaaacga
    26281 attagtcttt tcctttgtat taccgaccat cgatcaacga taccaagtgg aatcgatgag
    26341 tctcctaaac gtatcaccag agacaatgga aatcaatgtc caagatgacc gggtgctttc
    26401 tttgatccga cactggtcag tggatcgagt attctctgca gggcagatca cgatattcaa
    26461 tcacaaattg aatcaattat tagcccatgc aagaacactt ggttttaccg ttaatcagac
    26521 attactagac aatacgaagc cacttcgttt gaatcttcag agcgaatttg aattgacgaa
    26581 tgaagtattg gatgatattt ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa
    26641 gatcaacgaa actacttatc aagtacgatt agataaagga cagtctttaa cgataacaac
    26701 aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga gactacccac gatctgtcat
    26761 tgatttcttt atcaattacg aattcttggt cccattaatg gtgagaaagt cctgattgga
    26821 taagttggat taccagatgt cattcagata attctgttcc aaaggacttg cggcagttat
    26881 ccagaataat tgtcacaagt cttttctgta tgtactaaat ggacgtgaat aacttgctga
    26941 tgaaggaggg atttcattag gaatgtccac aaataaaata gcacagctta gcttattgtc
    27001 tgctgcttgt gtagcaggta ggatcgcttt tcaatttatt ccgaatttcc agccaatgac
    27061 tgctattttt ttatttatag tactttactt aagcttaaaa gatgcgttaa ttgtcatgag
    27121 tctttctatt gcgatcagca gtttttattt tggtgttgga ccatgggtca tcggccaatg
    27181 gatcagctat actgttgttc tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca
    27241 aaggaatcta tggttaaagg gagtctgttt ttttctggcg ggcatggttt acggaatagg
    27301 tatgaccgtt tttgatacgc tgttatatcg tttgcctcaa ccttggatct attaccttca
    27361 aggggtgtcg tttgatttga tgcacagtat aggcaatgtt gtgttctttc ttgtttttct
    27421 gccagtcgtc aaacgttttt acaatcattc aggaggaaaa aatgaaaaaa ataatttata
    27481 gtttgcttgc aatgagtgtc agtatactca ttttgacaag ttgcagtaca acctatacac
    27541 aatcggcttc caaccaaaag gaggaagtaa aaacatcgca aaaagcaaca atcacgttac
    27601 aagaaaatgg tcaaaacttt tcggaaaaag aagtggtctt caaaaaaggc gatgatttac
    27661 tgagtatttt gaaacgaaat tttgaagtca aagaagataa cggcttcatc acttcgcttg
    27721 aagggcactc acaggatgag aaaaatcaac tctattggat gtttactgtg gacgggaaaa
    27781 gtgctactac tggtgccaaa gagatcaaat tgaatgatgg acaatcggtc attttcaatt
    27841 tgtctaaact ataagtttca taaaaaactg ttaaagggcg aatattgcca taattcagtg
    27901 agttcagttg atgaaagaag gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt
    27961 ccgtaattgc tgctctaggg gctaatattc tagttgctat cagcaaattt gtcggctatg
    28021 caatcagtgg cagtgccgcc atgctaaacg aaagcatcca cagtatcgtt gattgcagca
    28081 atcagatttt gctgttattc ggtgataaac aagccaataa aggacagagc gagttgcatc
    28141 aatttggtga aggacgagca aaatactttt tcagtacaat cgtcgcaatg atgcttttct
    28201 tcggcggtgg ggcacttggg gtgatggaag cttttgaaaa gttgacgcat ccatcccatg
    28261 aagtgggtaa tccgatcatt gttatagcaa tactgttatt tggtttggta atcgaaggaa
    28321 gttcgttacg cgtggcaatg aaagaaatca aagaattgaa tactgaaaaa ctttccacga
    28381 tgcgcttttt gcgcgaaagt cgacacagtg aaatactgat tatttttacg gaagacttgt
    28441 gtgctgtaat cggtttggtc ttggctttag caggaacaat attgacttat gcaaccggga
    28501 ttgctgcatt tgatgcgatc agcggattgc tgatcggttt cttgctgatg ggagccgcca
    28561 ttttcctagc aaaagaattt tatagtctga tcatcggtga aagcgtgaca gcaagcgatt
    28621 tatcaaaaat caagttagct tttgaacgtc ctgagatcag tcggctgatc gatataaaaa
    28681 cagttcattt aagtccaacg gagattttag ttgcagcaaa agcagatatc gttggtaaca
    28741 aagagaatga agcttactcg gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc
    28801 ctgacaagaa gatgtatata tacattgaaa cagataaata tgacccaaat tattctcgta
    28861 aataaaacgt ttattcatga attgaagcaa aaatgagaaa aatataatct tgtttttaaa
    28921 taaaacggct acaatttagc gaatttatgt tacaatgagt ccgctgttta tttgttgtaa
    28981 ttaaaggaga tattttaatg aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg
    29041 cagtgatgcc actttgtata agctatatac cagtaggatt agcttgcgga gtattgttgc
    29101 aaaaagttgg atttgatcct ctcttatcag gactgttatc gattctggtc ttttccggtg
    29161 gcgcgcagtt tttagttgca tctatgctta cgacgcaagc atcatttgca acgacattat
    29221 tgatggtttt ctttttagaa ttacgctata cattattagg agcaagtctt gccggtttta
    29281 tgaaaaaaga aaagcggacg tttttggctg tcttttctca atcactgaat gatgaaaact
    29341 atgcggtgaa ctacttaaag ttttcaactg acccttcctg gaataaaaga aaggctttat
    29401 atgtcaattg gttctcaatg actgcatggg ccggtagcaa tatgatcggt aattttttcg
    29461 gctcggtgat tcgagtagat gctgaccttg tccattttgc tttgacggcc atgttcattt
    29521 tcatgtttat catgcagatg aaaagcgcac tactgatctt tacaggttta ttctcaggcg
    29581 tattaggggt cgtattcatg gtattattcc aaaatacttt cggattgatt gcagcaacag
    29641 tcattgcttc atttgccgga tttatgctgg agaaggcatt caaaaaagaa aaagctaaga
    29701 aactaagaga ccgcggtaaa gatgtcaatg agccgctctt caacttcccg gaacaggagg
    29761 ccaatcacca tgactaaact atatttgcta cttttagttg cttgtctgtt catcgtggca
    29821 tatatcccgc gcttgttccc gatgctttat tttactcatc gaaaagtgcc atcctggttc
    29881 agtgactgga tgaaatatgt accggtggca ttatttgcgg cgttagcttt taaagatgtt
    29941 ttcattactc atgaacatct ggatattgct tggaatatca aaatagctgc aatgattcta
    30001 gtagcaggcg tggcttacaa aacacgttcg atggcgttgt cagttttgac aggacttgcg
    30061 tctgtctttt tgctgtctat gctttaaact gttttatttt attaaaaaca gagattgcga
    30121 cataagttgt tcaaactgat ctatccgaac tgcatttgca acagtcaatt ctggatgcaa
    30181 gtttattcgt agggataaaa aagattagtg ttggcttatg atgcaagctc ttttttattt
    30241 taggaattag agagggattt gtccttctat gattttttgc tataataggg aagatgttta
    30301 ttttatgaaa gaatagttga gggaatttaa atgaataaaa aattgatgca aggaacattt
    30361 tggctaacct ttgctaatct tctatgtaaa gttttagggg tagtctattt gattccctgg
    30421 ctaagtatga tggggaacaa tcaagatggt atgcttgcta cgacattgta taatgttggc
    30481 tatcttcctt atggattgtt cctgatgctt ggtacagtcg gatttccaaa tgcgattgca
    30541 aaaaaagtag ctgtagccac aaaagaagga gataacgctg cttgtcggct gatttttaga
    30601 agtacgatca atatcatgtt tgtcatcggt atcgcctctg ctgcactgat gtatttattt
    30661 gctcctttac tagccggaat cagtccgatt gctaacgtta ataatgggat actagctatc
    30721 agaagtttat gcccatcttt gattgctatc ccaattctta gtgcaatgag aggatatttc
    30781 caaggaaaaa atgatctgcg gccttatgga acttctttga ttatcgaaca ggctgtgcga
    30841 gtgatcgtta tcttggctgg tacttattac ttgcgcgtat tgactgacgg aaccatatta
    30901 gaagctgtat tgatcagtac agtcgcttct ttcttcggtg ggttagctgc gattatccat
    30961 atgtatatcg tcggacgcag aaaagacttc ttcgaactta aagatttttt gatttccagc
    31021 cgttatttcg aaagagaaaa ccgatctgcc tctgtctcta tcattcggga aacactaccg
    31081 tttatttttg tcggctcagt cattactatc gttcaaatga tcgaccaagt aacgatgaaa
    31141 ccactgcttc actttttccg tccagagatc gcagatcagc aactggaatt cttattcagc
    31201 cgtgcttctg tcaatccaaa taaattgaca ttgatcctga tctccatggt aggaacagta
    31261 gctataagca gcttgccaat attaagcacg atgaagaaaa aagatcggat acaaatcgaa
    31321 aaaacagtag gagatagttt ttcgattgcg ttattgattc ttcttccttc attgactgga
    31381 atgtctttat tagctggacc actatacaca ttgttttttg gctatgatcc tgaaagtgtc
    31441 ggatatttcc aaatggcttt gctagcttct ttgtttttct ctttattcac gatcctatct
    31501 acgatgcttc aatctttgaa tcaccatcgt tttgcgatcc gattaactgt agaagcaatt
    31561 atcctgaaag tcatcttcca agtaatcgga ttaggattag ttggcggata cgggatgagc
    31621 ttatcaaatg gcttagcatt cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa
    31681 gaataccgaa tagcaccttt agcaaagatc agcaattttt tcttaaaaac tttccgctcc
    31741 accctgatta tgcttgcagt atgttttgcc gttttctttt tgctgaatca gcgattgtca
    31801 atggaatcaa aaagctatgc aatgatttat tgtgtggctg taggaagtat tggcggctta
    31861 gtatttttat tttctcaatt cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat
    31921 catagcagaa gcaaagaata ggttaactaa tcgttttttg ctgtcacttg aaaaacggca
    31981 actcgtatga ttacgcttgt cgtttttcta ttttccaatt ttagtttcat atctaatctt
    32041 acttatgata tagttataag gtctggaaaa attctttgaa acgaggaaat taaacacatg
    32101 aaacttgcag tagttacaga tagttcggca tatctgccgg aacgtatcaa gaaccatccc
    32161 gacttattcg ttattccaat ccctttgatt atggatggga aaatttataa cgaaggaatc
    32221 gatatcgaag cggatgaata ttatggactt ttaaaaaaca gtaaagattt tcctactact
    32281 tcacaacctg tcgtagggga agttttgggt ttatatgagg aattgaaaga gaaagggtat
    32341 gacacgattt taagtatcca tctttcttca gggatttctg gctttgtcaa cacattattt
    32401 gcgatgaaag atgacatcag cggagtaacg gtcattcctt atgactctaa gatcactagt
    32461 atgccgatgg gacacatggt agaagctgcc ttagacttaa atgatgaagg tcagtccctt
    32521 gatgaaatac ttgcacatat cgatcgaatc cgagataata cctatgctta tctgattgta
    32581 gatgatttga acaatttagt acgaggagga cgtttgacaa atggtgcagc gctgattggc
    32641 agcttattaa aaatcaaacc gatcctgaca ttttctgaag ggaaaattgt tttattcgaa
    32701 aaaatccgtt caagtaaaaa agcatttgct cgagctgaag aaattattgg tagaagaaat
    32761 aacgaaatac aaccgccagt caaactttac gtgatacatg caaataattt agaagtagcg
    32821 attgcggaga aagagaaatt gcagaaaaaa tatccaaatg cagaaatcga aattggtcat
    32881 ttcggaccag tcattggtac acatttaggt gaaaaagcca tcggcattgc catttcagcg
    32941 caataattcc ttttattcaa aaaacatacc aaaaata
//
LOCUS       DAYYBH010000023        32502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000023 DAYYBH010000000
VERSION     DAYYBH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.023"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACHAUG_001265"
     CDS             complement(<1..563)
                     /locus_tag="ACHAUG_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFM8480370.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACHAUG_001266"
     CDS             complement(750..1400)
                     /locus_tag="ACHAUG_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480371.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACHAUG_001267"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACHAUG_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8480372.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACHAUG_001268"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACHAUG_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8480373.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACHAUG_001269"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACHAUG_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8480374.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACHAUG_001270"
     CDS             complement(4926..5657)
                     /locus_tag="ACHAUG_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8480375.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACHAUG_001271"
     CDS             complement(5661..6926)
                     /locus_tag="ACHAUG_001271"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8480376.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACHAUG_001272"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACHAUG_001272"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8480377.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACHAUG_001273"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACHAUG_001273"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8480378.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACHAUG_001274"
     CDS             12268..13770
                     /locus_tag="ACHAUG_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8480379.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACHAUG_001275"
     CDS             13772..14509
                     /locus_tag="ACHAUG_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8480380.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACHAUG_001276"
     CDS             complement(14680..15873)
                     /locus_tag="ACHAUG_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8480381.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACHAUG_001277"
     CDS             complement(15952..16158)
                     /locus_tag="ACHAUG_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8480382.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACHAUG_001278"
     CDS             complement(16151..17293)
                     /locus_tag="ACHAUG_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8480383.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACHAUG_001279"
     CDS             17805..18440
                     /locus_tag="ACHAUG_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8480384.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACHAUG_001280"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACHAUG_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACHAUG_001281"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACHAUG_001281"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACHAUG_001282"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACHAUG_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFM8480385.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="ACHAUG_001283"
     CDS             21086..21253
                     /locus_tag="ACHAUG_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8480386.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="ACHAUG_001284"
     CDS             complement(21313..21666)
                     /locus_tag="ACHAUG_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480387.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="ACHAUG_001285"
     CDS             22170..22589
                     /locus_tag="ACHAUG_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8480388.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="ACHAUG_001286"
     CDS             22615..22821
                     /locus_tag="ACHAUG_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480389.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="ACHAUG_001287"
     CDS             complement(23049..23222)
                     /locus_tag="ACHAUG_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480390.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23234..23863
                     /locus_tag="ACHAUG_001288"
     CDS             23234..23863
                     /locus_tag="ACHAUG_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480391.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACHAUG_001289"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACHAUG_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8480392.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACHAUG_001290"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACHAUG_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8480393.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACHAUG_001291"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACHAUG_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8480394.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACHAUG_001292"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACHAUG_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8480395.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACHAUG_001293"
                     /pseudo
     CDS             complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACHAUG_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(30257..30484)
                     /locus_tag="ACHAUG_001294"
     CDS             complement(30257..30484)
                     /locus_tag="ACHAUG_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8480396.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31575
                     /locus_tag="ACHAUG_001295"
                     /pseudo
     CDS             30968..31575
                     /locus_tag="ACHAUG_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            31748..32461
                     /locus_tag="ACHAUG_001296"
     CDS             31748..32461
                     /locus_tag="ACHAUG_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480397.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9521 a         6618 c         5736 g        10627 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt
    31741 attttatatg aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg
    31801 tacgtctcct agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag
    31861 acaaaccagt tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac
    31921 atcttcaaca agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt
    31981 tgagacaaat atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat
    32041 agatccgtta gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat
    32101 tagaggtaat acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc
    32161 tgcaggaaca cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt
    32221 tatgctcact gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg
    32281 gacaatcact agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta
    32341 tccagcgttc attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat
    32401 tcctacagga aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta
    32461 gactttctaa tatgttatac aaaaagaagc tgacagcatt tt
//
LOCUS       DAYYBH010000024        32099 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000024 DAYYBH010000000
VERSION     DAYYBH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.024"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..1556
                     /locus_tag="ACHAUG_001297"
     CDS             129..1556
                     /locus_tag="ACHAUG_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480398.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1648..2013)
                     /locus_tag="ACHAUG_001298"
     CDS             complement(1648..2013)
                     /locus_tag="ACHAUG_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8480399.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2010..2375)
                     /locus_tag="ACHAUG_001299"
     CDS             complement(2010..2375)
                     /locus_tag="ACHAUG_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8480400.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2567..2815)
                     /locus_tag="ACHAUG_001300"
     CDS             complement(2567..2815)
                     /locus_tag="ACHAUG_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480401.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2814..2942
                     /locus_tag="ACHAUG_001301"
     CDS             2814..2942
                     /locus_tag="ACHAUG_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8480402.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2999..4693
                     /locus_tag="ACHAUG_001302"
     CDS             2999..4693
                     /locus_tag="ACHAUG_001302"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8480403.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4793..4966
                     /locus_tag="ACHAUG_001303"
     CDS             4793..4966
                     /locus_tag="ACHAUG_001303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480404.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5344..6477
                     /locus_tag="ACHAUG_001304"
     CDS             5344..6477
                     /locus_tag="ACHAUG_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8480405.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6508..6828
                     /locus_tag="ACHAUG_001305"
     CDS             6508..6828
                     /locus_tag="ACHAUG_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8480406.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6857..7321)
                     /locus_tag="ACHAUG_001306"
     CDS             complement(6857..7321)
                     /locus_tag="ACHAUG_001306"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8480407.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7453..8706)
                     /locus_tag="ACHAUG_001307"
     CDS             complement(7453..8706)
                     /locus_tag="ACHAUG_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8480408.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8735..9052)
                     /locus_tag="ACHAUG_001308"
     CDS             complement(8735..9052)
                     /locus_tag="ACHAUG_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8480409.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9078..9398)
                     /locus_tag="ACHAUG_001309"
     CDS             complement(9078..9398)
                     /locus_tag="ACHAUG_001309"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8480410.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9862..11277)
                     /locus_tag="ACHAUG_001310"
     CDS             complement(9862..11277)
                     /locus_tag="ACHAUG_001310"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8480411.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11294..12610)
                     /locus_tag="ACHAUG_001311"
     CDS             complement(11294..12610)
                     /locus_tag="ACHAUG_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8480412.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12760..13458)
                     /locus_tag="ACHAUG_001312"
     CDS             complement(12760..13458)
                     /locus_tag="ACHAUG_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8480413.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13747..14736)
                     /locus_tag="ACHAUG_001313"
     CDS             complement(13747..14736)
                     /locus_tag="ACHAUG_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8480414.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14816..15649)
                     /gene="msrA"
                     /locus_tag="ACHAUG_001314"
     CDS             complement(14816..15649)
                     /gene="msrA"
                     /locus_tag="ACHAUG_001314"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8480415.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15824..17458)
                     /locus_tag="ACHAUG_001315"
     CDS             complement(15824..17458)
                     /locus_tag="ACHAUG_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8480416.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17445..19133)
                     /locus_tag="ACHAUG_001316"
     CDS             complement(17445..19133)
                     /locus_tag="ACHAUG_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480417.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            19279..20916
                     /locus_tag="ACHAUG_001317"
     CDS             19279..20916
                     /locus_tag="ACHAUG_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480418.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(21061..21801)
                     /gene="truA"
                     /locus_tag="ACHAUG_001318"
     CDS             complement(21061..21801)
                     /gene="truA"
                     /locus_tag="ACHAUG_001318"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8480419.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(21992..22627)
                     /locus_tag="ACHAUG_001319"
     CDS             complement(21992..22627)
                     /locus_tag="ACHAUG_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8480420.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(22774..23994)
                     /gene="sstT"
                     /locus_tag="ACHAUG_001320"
     CDS             complement(22774..23994)
                     /gene="sstT"
                     /locus_tag="ACHAUG_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8480421.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            24213..25211
                     /locus_tag="ACHAUG_001321"
     CDS             24213..25211
                     /locus_tag="ACHAUG_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480422.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            25407..27242
                     /locus_tag="ACHAUG_001322"
     CDS             25407..27242
                     /locus_tag="ACHAUG_001322"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8480423.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(27302..28018)
                     /locus_tag="ACHAUG_001323"
     CDS             complement(27302..28018)
                     /locus_tag="ACHAUG_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8480424.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(28245..28787)
                     /locus_tag="ACHAUG_001324"
     CDS             complement(28245..28787)
                     /locus_tag="ACHAUG_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8480425.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(28792..29019)
                     /locus_tag="ACHAUG_001325"
     CDS             complement(28792..29019)
                     /locus_tag="ACHAUG_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8480426.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(29098..30978)
                     /locus_tag="ACHAUG_001326"
     CDS             complement(29098..30978)
                     /locus_tag="ACHAUG_001326"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8480427.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(31167..31781)
                     /locus_tag="ACHAUG_001327"
     CDS             complement(31167..31781)
                     /locus_tag="ACHAUG_001327"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8480428.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            31923..>32099
                     /locus_tag="ACHAUG_001328"
     CDS             31923..>32099
                     /locus_tag="ACHAUG_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8480429.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT        10124 a         6271 c         5235 g        10469 t
ORIGIN      
        1 tataacataa aaaacctttt ttaacggcat ttatatgctt tttcagaaat taactgatga
       61 tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag
      121 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
      181 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
      241 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
      301 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
      361 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
      421 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
      481 aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg
      541 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
      601 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
      661 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
      721 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
      781 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
      841 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
      901 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
      961 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
     1021 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
     1081 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
     1141 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
     1201 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
     1261 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
     1321 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
     1381 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
     1441 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
     1501 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
     1561 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
     1621 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc
     1681 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
     1741 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
     1801 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc
     1861 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
     1921 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
     1981 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
     2041 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
     2101 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
     2161 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
     2221 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
     2281 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
     2341 cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt
     2401 tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct
     2461 aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca
     2521 taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca
     2581 gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac
     2641 catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag
     2701 acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta
     2761 ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta
     2821 aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag
     2881 gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct
     2941 agcatcctat ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat
     3001 gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta
     3061 tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca
     3121 cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca
     3181 tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac
     3241 ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac
     3301 tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt
     3361 gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg
     3421 tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat
     3481 cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat
     3541 gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat
     3601 gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg
     3661 ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg
     3721 caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga
     3781 tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt
     3841 tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag attcagctac
     3901 tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct
     3961 ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga
     4021 tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa
     4081 tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac
     4141 cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca
     4201 accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc
     4261 aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct
     4321 agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa
     4381 agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc
     4441 cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac
     4501 caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca
     4561 aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg
     4621 tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa
     4681 aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac
     4741 caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc
     4801 ttttgaacac cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc
     4861 agccaagcta aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca
     4921 agcttttgaa caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag
     4981 aaaaattttg tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa
     5041 ctgatccctt ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat
     5101 tttttcaacg tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat
     5161 gattaccatt acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta
     5221 tagaatgttt catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa
     5281 aatgaaatta tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct
     5341 ccaatgaaag cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat
     5401 ccaaaaatat tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc
     5461 tccgatctgc atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga
     5521 cacgaaccaa tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg
     5581 caacgtgtgg ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac
     5641 atggaaagcc aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt
     5701 ggcaagttga acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca
     5761 gactctactt catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta
     5821 tcagatattt tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat
     5881 acagttgtga ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg
     5941 gctggagcag agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct
     6001 aattacaatg gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg
     6061 tatgaactgg ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta
     6121 gagcctgtca aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca
     6181 ttacagatgg catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat
     6241 atgactccag cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag
     6301 catggacagg caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga
     6361 ctattcgatc ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca
     6421 tatgagatct ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt
     6481 cagtaaaaaa tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc
     6541 gaaataggaa aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa
     6601 aatcgttaca cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt
     6661 ggaagccttg cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct
     6721 ggtgctattt tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac
     6781 gacgaaaaaa aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa
     6841 tctcatgcct cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac
     6901 tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat
     6961 ctgtaattaa gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta
     7021 atatccgctt ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat
     7081 ttggtgtcag atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga
     7141 tggtttcttt ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc
     7201 cagtacgaat actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat
     7261 tcgcgctttg ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca
     7321 atgtttaaac ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa
     7381 ttagcaatga caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg
     7441 ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt
     7501 gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc
     7561 tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat
     7621 aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac
     7681 cattggtgtc aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa
     7741 catcgttggt tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat
     7801 atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc
     7861 tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct
     7921 atgtccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat
     7981 caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa
     8041 tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac
     8101 tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa
     8161 agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc
     8221 cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa
     8281 gatttctgtt gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc
     8341 taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc
     8401 ttttcggtag ccgttagcta aatgataagc aagcaagaaa gcaatcaaga cagaaagaat
     8461 ggcaaatgta ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat
     8521 tgagttcatc aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc
     8581 tcctaagatc attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg
     8641 ttgtgcacca atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt
     8701 atccataaat cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat
     8761 caatgatctc ttttgctaaa tctgtaaaag ctatagatgt catcagatga tcttgactgt
     8821 ggaccgtcaa aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg
     8881 tttgagagtg atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta
     8941 aagtgaaatc accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc
     9001 cgccaaacat gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta
     9061 tcctccgata atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca
     9121 ccattcatca tgccatagtc agccatattg attacatcta aaggaatgcc tttaggtgct
     9181 agcttttcag caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct
     9241 acatttttag attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct
     9301 tccattcctt ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt
     9361 ccagctgaac atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata
     9421 cttacacttg tatataagca agtagcgtgc caactttaaa agcgctttaa aaagttgata
     9481 tcatcacatt taaggtctat accaagttgc atacacacta aaaaagaaag cgcacactaa
     9541 acacacaaat taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca
     9601 caaaacttgt cacaactttt ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat
     9661 aagtccaacc aagttaaaaa cataagatgt tgtttttact ttgctattct tattacttga
     9721 tgcaggacag ctttttcaca acctctcaat aaacggtgtt caatcagctg accctgggta
     9781 ttagctcaaa acgctggatt cacaacctct tttatttcaa ataaccagta aatacaatga
     9841 acatctacaa aaaacttcta tttatttata gatttcgtca ggcaaatcca attctctctt
     9901 atcaatcaaa gtctgatacc acgaagcaga ttttttcaat gttctgcttc gttcatcgtt
     9961 caattcgatt cgaactaatc catatcgatt cttaaatgca ttcattggtg acacacagtc
    10021 agtgaatgcc cataacatat agccttcgca atttgcgcct tcttctgttg ctttgatcaa
    10081 gtaatgcaaa tgctgtccga taaattcgat acggtaatca tcttgcacga ttcctttctc
    10141 atccatatac tgttcttcat attctcttcc cataccgttt tctgttatca gccaagggat
    10201 attatgataa ttttctttta gatacatggc tatatcatac atgattttcg gatagatttc
    10261 ccatcccctg gaattattca ttttcttccc aggtaaatca aagacttcat aatatcgttc
    10321 aggatggaaa ggtgtctctt cattccattg ataacgaggg gcgcgaacgc gtttaggata
    10381 atattgattg attccaagga aatctactgt atgatgctga atcgtttcta aatcctctgt
    10441 tgcaggatcg aaataaatcg attgtttttg acacaactcc aggaactctt ctgtgtagta
    10501 tccttttacc atcggatcaa aaaatactcg attataaaac aaatcataca tataagcagc
    10561 tttttggtct tctggagaat tggatcgagc atagaccatt tcaggattta aaatacagcc
    10621 aatccttcca ccataatttc cgctgcggta aatctctact gctttagccg ttgctaaaac
    10681 tttatgataa ttccacatca tccacttttt tgtgttttgt tcatgcggcc agcgaacagc
    10741 atccaaatag caacgcgttt ggggaacgat tggttcgttg aatgtaaacc agttttttac
    10801 cctatcaccg taacgatcaa aggcaatttg agcatactta gcataaaaat caactacttt
    10861 tcttgaagac cagccatcat atttttccaa aagaaaagct gggacttcat aatgttccaa
    10921 acaaatcatc ggttctacgt ctgcttcgat caaagagtcg atcatttcac ttatatgctg
    10981 agcatattct tcatctacta tacagttctc ataatcagta aaaaaacgag cccaattgat
    11041 agaagtccga taatgtgtca gatggatgga tttcatcaga tccacatcct gctggtattg
    11101 ttccataaaa ttagttgcta cggctgggcc atacccatta tgccaaacaa aatgttcatt
    11161 tttataccag ctatctataa acgaatcctg tccttctttt tttcctttcc aaccttccgt
    11221 ttgccaagca gaagatgcag caccaagaat aaaatttttt ggaatagtaa ttttcatgat
    11281 ttcctcctgt tttttagatc ccatcatttt ccacacgatt tgctactttt acaaatggca
    11341 gatagatcaa aatagaaaca ataatacaaa caagttgcgt aataactgca ccgatatttc
    11401 ctgctgttgc cagataagca ttgacgatcg gcggcattgg ccaagggacc attacaactg
    11461 ctttcgccgc aaatcctaaa gcggttgcaa aataaccaat cgttccagtg ataagaggcg
    11521 ttaagataaa cggaatagct aaaattggat tcagcatgat cggcatacca aaaataactg
    11581 gttcattgat gttgaagatc ccaggagcaa gggataactt ggtgatttgt ttcatatctt
    11641 cccgttttcc aactaacaat actgcaatca gtaaaccgat cgtgactcca gacccgccca
    11701 tactcatata catatcccaa aatggcatag taataatgtt cggtacttct tttccagctt
    11761 caaaagcttc cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt atcggcttca
    11821 ccatttgatt tccatggata ccaatcaccc aaaagacttg cgcaataaac atcaatagca
    11881 aaatacccgg taacccttgt acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga
    11941 tatcataaag gtacattcct gtaagttctt tgatgataaa tcccgcagcc gcgatacccg
    12001 atacagtcag aatcgtcggg aataaagcag aaaaactagc agaaacattc gttggcacgg
    12061 tatcaggcat tctcagctgt agtttcttct gtttcctcaa ccagcaataa agttcaatag
    12121 aaaggatgga gaccaacatt cccaaaaata accctttcgt atctgtatat tgtttagcca
    12181 atacgttttc tactataaca gtctgatttt cattcactaa ttcataaaca gttggattaa
    12241 cagataaata agacatcact gcgatgatac cagggaatac accatccact ttattcagct
    12301 tggctaattc gatccctatc agaaaaacag catagatggt caagaaactt agtgtgacat
    12361 agctgatggc agatgcgata ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg
    12421 ctaagccatt ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa
    12481 taattaccgg aatcaatgca gcaaatgcgt tttttatcac catgatatac cttaaattat
    12541 tgattctcgt tgccacgttc cctaaaacat tggctaattt atccataaaa ctcattccag
    12601 atcccctcat tcctgcttct tctccttgac gtcatctctt tttgtctatt agtcagcgct
    12661 tacatttcga tgaacagaaa ctatttactc ctctttttta ccaaatataa aacaaaaatt
    12721 catctgatgt atttatcata aaatacatca gatgaactgt caattagttt ctttttctcc
    12781 tattattttc tgtaattctc tccgatttat tgccatatgg acagtcattg catcaaacgc
    12841 agcaagtgca ttccgccttt cgatggcctg aacgatctct gtatgaatat cgatcgtctc
    12901 gattttcaga tgctggatat cataattttt attaaacaaa gcaattgatt cattaatgat
    12961 tgggataata tgcagcaagg ctacgtttcc gctagcttct gcaatcatcg tatgaaaatc
    13021 gatgtctaat tgaacatgtt cttgtgtgcc ttctcgaaaa gaagtttcaa tcttttgcgt
    13081 taattttttc atttggacga tttgattctt cgttgcatat tccgctgcca aacctgccat
    13141 cttaggctcc aataaatatc tcagttcaaa taagtctcgt atcatccgat cctgatcttc
    13201 aatcaaagaa aaaccgagtg ggtcattggc aatccctcgc ttatcactga caaatgtccc
    13261 cgctccttgc cgaacttcaa ggatattcct ggaagctaga gcccgcacag cttctcgcac
    13321 cgtgcttcgc cccacctcta aataatcgga caattcatat tcattcggca gtttatctcc
    13381 tacttgcaac tgcatttcct ttaaatatct aaggatcttg tcagtagtta cttctactag
    13441 ggttttattt tcttccaaac cacatacctc cttttcatct gatgtatttt ttcgtaatta
    13501 ttataacacg tttgaatcat ctgatgattt tttgtataca aaattgatta atccgttcat
    13561 agacatacaa acgaagaaga tatttaccat aaaaaagact aggataaaat tcactcctag
    13621 ccttcgatca tttttatttt gaacgacaaa aaatatgatg ttcaataaca gacgatcttc
    13681 attcgattct tgatgtcatc ttttgttttt tttaatatat ggataaaaag tttgattaca
    13741 tcgtttctat tctagaattt ggtcgattct caattcttct tctggtgaga aatctaaatt
    13801 atccatcatt cgaacacttt cttgtaactg acttaaccga cttgctccga ccagtacact
    13861 tgtaacagat ttttgtcgca aattccatgc aagggccatt tgcgctaatg attgtcctcg
    13921 tgatacggcg attgtttgca gtgctttgat tttttccaga gttgatccaa cttgttcttc
    13981 tgaaaggaat ggtatttcct tacgatgtgc tctagaatct tcaggaatac catgtagata
    14041 acgatttgtc agcaaccctt gagcaagcgg actgaacgtg attgccccta gcccttcttc
    14101 ttctaatact ggagatagat cgtcttctag tagtgcacga gaaaacatat tgtagcgcat
    14161 ctgatgaata atgaacggtg cttcctttct ttttaaaatt tcagtcattt tttttgtatc
    14221 ttctccatta tagttggata taccaatata taatgctttt ccttgtctta caagttgcat
    14281 caaagcttct gctgtttctt ccaaaggtgt atcagggtct gggcgatgat gatagaagat
    14341 gtcaacataa tctagcccca ttctttggag actttggtca caacttgaaa tgatggattt
    14401 acgagagccc cattccccat atggcccttc ccacatatga taacctgctt tgctagaaat
    14461 aatcagttca tcacggtagg cttgaaaatc ttctctaaaa atacgcccaa agtttttctc
    14521 tgcagctcca gcagggcctc cataattatt cgcaaggtcg aaatgggtaa ttcccatgtc
    14581 gaatgcgcct cttaaaatct ctctttgatt atggatagga tcataatcac caaaattttg
    14641 ccataatccg agagataaaa tcggtaattt caaacctgaa tgaccggatt tacgatattc
    14701 cattttttca taacgtgcac tatctgcaat atacaatttt tctcctcctc atcaaatctt
    14761 tttaaaacgc tttctttttt ctatttttag tttactacat tttaaagaaa aggagttaat
    14821 caaaatgatt ttttcgtata tttttttgcc atttgttttt tactcgttga tagatcagaa
    14881 attgattacg tgcttgctgg atttttttat aacgtttcgg ttgttttttg taaaattgct
    14941 gatgatagtt ttctgctggc caaaacgtac cggctggttc gatttttgtg acgattggtt
    15001 ggtggaaagt tcctgaactg tctagtttct gttttgattg ttccgctaat tctttttgtc
    15061 gttcgacttg ataaaaaatc actggacgat attgcttccc tctatcttga aattgtccaa
    15121 atgtatctgt gggatcagta atttgccaat agattgtcag caactcgcta taacttattt
    15181 ctcttgtgtc ataaatgatc tccacagctt ccacatgacc tgtgtaacca ccacttactt
    15241 gatcataatt tggtttttca acatgtccgc ccgtgtatcc tgacaaaaca gataggatcc
    15301 cttttttcga ttcaaacggc tcgaccatac accagaaaca tccccctgca aatactgcac
    15361 gttcttgata gtcagcatct tgcatggcat gcttggaaaa gtcatatttt tgctccttat
    15421 caaatccttc cgtaatcaat aaataaaagt ccattacatc aggcgtcaga ttcattcgga
    15481 tagctaatgg acgtagtgtt gcttctagtt ttgataactg ttctttaacg gatatatttt
    15541 ctcttatgtc attctttgtc tgaatcaata cttttcgttc ccaatctcgt gtattcggat
    15601 tcaaaatcag attataaagt gtttgaatga atatttgttc ctcatccata gattctcctc
    15661 ctttccgaag aagacaagct agtgacttta gctgtaattt atcataaagt gaggaaataa
    15721 gcgattactt catcccccta cagatttttc gtataaaaaa gagagaatcc ttatcgacag
    15781 attctccttt ttaatcaatt tattttataa atgaaaagtt aatttacctt ttatggaaaa
    15841 ctattttaaa aaataaacat aaatatgcag ttaatgtaac aagataagaa ataatagtcg
    15901 ttactgtata acttgtgctt actttaatat cagcaactcc tttgactccg cttatttgta
    15961 ccaatccatt agcattcacc tgctccttta caggttgatg atttatttct acattcgttc
    16021 ctaaataagc atatacagga agtgtgatta cttggctctt ctcattagtt actctatata
    16081 tgatagaact accagttgtt ttataaggaa ggtttaccca ttgactgcca atctttgctt
    16141 cgtgttttat tatatgctcc ttattctcca atgaagcgat aggagaataa tcatataaaa
    16201 aataatccat tcctttagtg gtatacgcct ctactgtttc tttgtttaca acaatatgtc
    16261 ccggctgaga aattattttt tgattgaaat tttttttact agaaacagtt aaagtgatta
    16321 ataacaaaac agtcataaat aaataaaata gttctcttgt cttattgtat cttctatttt
    16381 tgaaaaatat catcgttaat atcagagaag agaatattat ttgcaagctt aagatccgcc
    16441 aaggaaattg taacaatttg gctggcgtat tttgcagaag ataccatggc accaaactag
    16501 ttgtagctag ccaagtcaaa cacgtaccat aataaatctt tttttctatg tttgttaatt
    16561 ttttaaataa aatcagggga atacataaag ataacagacc aactagtccc attgagtaag
    16621 tagttaagtc gttgttcata gcttcagtca gactatcgaa cacattagat gcatgagcat
    16681 ataaatttgt cacgctaggt ttgttgattt tttggtagag catttgttca aacatcggat
    16741 accaaaaagc actcgtcaga accaatgtca ttcctgccgc actaaataat tttttaaata
    16801 tagcaataaa atctgcattc ttgaaacctt tagaaaccaa acgaataaaa ataaaaatga
    16861 taattaatgc tgtactcatt aatgttgata gtacatggct gtacactagc aaactcatac
    16921 tcaatgctag ctttacagac ggatactttt cttcttttcc agagatgatt tgataagcat
    16981 aaaataatac agggataaca agtgttattg cgatagcttc ggcaattgcg gaacgatagt
    17041 agatgtctac tgtccgataa gcagagaaaa tatagagaca actaaataaa aatgccgctt
    17101 ttttttgttt taaaaaccgc tcgccataat gataagctac gagtgttgta cacaagttca
    17161 ataaccaaac gtaaataata tagcttacaa tcaaattttt tgtcatccaa tataacatta
    17221 cagctggaaa aaaagtcaga aagggataaa aataatttac accatatcca gtataattga
    17281 atgtctcata attaatcgga ctggtaaaaa tagtcgataa ccccttgatt cgagataagt
    17341 gaaaagaaag atctccagtg gtataaatac tctgtgtatt tttgaaaaag atgaaagaat
    17401 atatactact aactataatt aatccactca atattactac ttttttattt attttcattc
    17461 ttcttgttta tctccttttt agtcacatat cgagaatctt tcaacttatt aggtgcaaat
    17521 cgtttaaaac ttgaatacac cactaaacaa taagtcagaa tactgatcca taaagccatg
    17581 agagcagttg gagtatattt tgttgtaatt tctatcttat tttctccagc atttacatcc
    17641 acattcacta agccatgttt tttataagag tcatttacga ctttaccgtt tacttttacc
    17701 tgtgtaccta aataagcata tactggtact gtcacttttt gctttttgtt gcttgccact
    17761 gtgtaagtaa tgttattatc tgcagcttta tacttccctt tttcccacga ttctcctact
    17821 tttacttcat gtttttctaa gtgaggcctt tcttttatcg cttctgttgg ggcgtaatcg
    17881 tataatcctc ccatatctga tgttgtataa cgtgggagtg tttcttcatt gataacaata
    17941 tgaccgtgtt ttgattggat aacttttgcg taattttctt ttgctgctat agacgtgacg
    18001 agtagaaaca ccacagtagc gccaatgatt aaccatgaat attttttatt catagtcttt
    18061 ttcataaaaa caagcgttat gatcattgaa gaaaaaaaga tttggatacc taagatacgc
    18121 caaggaaact ggagtaagct tatcggtgtt ttttgaaaca aaaaccatgg aaataaatta
    18181 gttacgcata cccatgtaga aactacgcca acatagatga ttttttcttt tttctccatt
    18241 tctttcagta tgaataaagg aaaaatcaac gcaaccaatc ctaataaccc catagtgtat
    18301 gttgtaatat cgttatttaa cgcttgaatg atactatctg aaatagaaag tgcttctagc
    18361 tgcaaatctg ttttgaaggg actgtgtatt tcttgataat tcatctgctg caacatagga
    18421 taccaaaaat agctagttaa tgcacaagtc aaaaaaacag cctttactaa attcacgacc
    18481 acaaatttta tatcgttttt tatttgcttg cttaaaaaca aacgaactat aatcatacaa
    18541 gcaatcaatg cactacacaa tagtgtagtt aagacatgag tatacacaag tagacttata
    18601 ctgacagata aagcgatgac ttttttttct ctattgaata tcaatccata agcataatat
    18661 aaaacagctg ggataatcgt aagagcgatt gcttctgcaa ttgcggatcg ataatagata
    18721 tcaactgttc gataagttgc aaaagtatat aaacaactaa ataaaaaagc agcatctatc
    18781 tttttaaaaa acttttctcc ataataaaaa ctaatcagaa tcgtacaaat atttaataac
    18841 caaatatata gaacataact taatattaag ttatttgaaa ttccataaaa aacgactgca
    18901 ggaattattg taagaaaagg ataaaaatag ttcagtccat ctccataatt gttaaatgtt
    18961 gtatagttga ttggcccttc aaaaatagaa gataaccctt tgattcgtga aagatgaaaa
    19021 gataaatcac ctgttgtata aatttcttga gcatttctca taaaatttac tgaatataaa
    19081 gtactaagta agataagaac tactaaaaga aatgcttttt tacttatttt catagatttg
    19141 ttgttctcct tttttaattc tattttcagt atactagaat tgtctttttt taataagttt
    19201 tgcgttaaat ttttcatagc actagtatga tatactagaa aagaatcaat ttttattact
    19261 tttaaatagg agaaaaaaat gaaaaaaaac aaaaaccaaa taatagatat catttttatg
    19321 ttatttctaa gttttgctta cgtcatccct ctattcatgt ctaagggaat ctatcatagt
    19381 gtcaatcagg atacttactt tcatttatct cgaatcatag gactagataa tgtatggagt
    19441 tctccagtaa acttcaataa cttcgatcat catggaacaa tgatgaatat tttttatcca
    19501 tggcttactc tttatccagc ttttttattt tataaaatga tgggaaatct tgttttaagt
    19561 tataatatct actatttttt tatcactttt ttaacaatgg tcgtttctta tttttctatg
    19621 aaacaaataa aaaacaatcg ttatatttcg ctgttatttt cgattattta tacattttcc
    19681 gcctatcgtg ccattgacat attccatcgt gcttcattgg gagaagctgt cgctttaaca
    19741 tttttgcctt tgatactaat ggggtgttac gaaatttata ttagagatta ccaaaaatgg
    19801 tactggttgt ctattggcat gacgctagta gtgtatactc atttactttc tgtcgctatg
    19861 gtttctgtat ttattggagg aacgttgttt ctttcttttt atttttggga ccaaaaaatc
    19921 gctagactct taagtctttt aaaagctacc gtcctcacct tttttttaag tgcgggattc
    19981 ttaattcctt ttattcaaca atctagagca caagaattga aagtcccact gggaaaagaa
    20041 ttaagtggta tggctcctag tgatatgctg acacatattt taaataataa ctataacaac
    20101 tatactattg gattattctt atttctcgga ttgattggtg cttttatttt tataaaaaaa
    20161 ttaactgctg atgatttatt tattttcttt cttggtgtat ttgttctttt ttgttcaacc
    20221 aatttatttc cttggcaact ttttaaccat acaccagtta agagtctaca atttgtctgg
    20281 cggttaaatg gtttttcttc tttatttata gcctatacga tgagtatagt gatctattat
    20341 attttttcta ctaaaaataa ttcatggaaa ataatggtct ttacgtttct tgcactaata
    20401 ttgcatctct caggagtaag caattctatc catgctaaca aagattcatt aacacttata
    20461 gcacctgaag acgctgttgc tatagctgaa aattacaatc atactgatta tgcaaacaaa
    20521 gagtcaatct atcatcctga tatgattaat aatgatcaat atttactagg taaccaggaa
    20581 ataaatccaa caactcattt tatgagtaac aaacttacaa ttgaattaga taattcgaat
    20641 aataaaaata ctgtcttgac gacacctatt tatcgttata aagggcaagt ggcttctatt
    20701 aatggcaaac tagtacaaac aaaattatct aagtttggca caactgaatt aactattcct
    20761 ccaggtataa ataaagtcgt tattacttat cagtacacaa aactagcaat tgcttcaaga
    20821 tatttatcta tcgttacttt gattttattt ttactttatc gatttacttt ttctaaacaa
    20881 aaacaaccga gaagagaagt acaacattct cactagattt ttatttaaga aagacttttt
    20941 agctgtacac ataaacaggc taaaaagtct ttttttatat ttctttaatt tgcttcatac
    21001 attatttaac atcgatattt atactgtgaa acatgcgttt catccattat caaagtattt
    21061 ttatcgataa taaacttcat ccaaaaacag accttgtgca gggacggttt ctcctgcatg
    21121 ttgccgcact ttgctttcaa atatttcatc gattgtttct atcggcatgg cacctgtgcc
    21181 gatttctagt aatgtcccca tgataatccg caccatttta tataagaatc cgtctccgac
    21241 aaaagtaaaa tgaagcattt ctccctttcg ttcgatagat aattcatcaa tcgtacgcac
    21301 tgtggatttt tttgattttt tcaatgcaga aaaaccaatg aaatcatgtg tacccactaa
    21361 tttttcgcaa gctgcattca gcttgtccat atccaactct tgagggaaat aaaagctgtg
    21421 gtttcgctca aatgctgaag gaatcacgtt gttccaaaca taatagctgt attttttgcc
    21481 caccgcatta tatcttgaat ggaagcgctc aggcatttct tcgattcttt tgatgacaat
    21541 atcccgcgga aggtaacgat tcataaagtc gatcatttct gccagtccaa tttctgtgtt
    21601 tgttttaaaa tttgctactt gtcctcgcgc atgggtgcca gcatctgttc ttcctgatcc
    21661 aataatttct atctcttcat ttgtcatctg atgcaatact ttttcgattt ttccttgaat
    21721 ggttttatcg tcatctccaa gtctttgcca tcctgaatat ctttttccgt catattcgat
    21781 tgtcaacttg atgtttctca tattttagtg gctcctctgt cttccatttg gtcttctctc
    21841 taccttttta agattgtagc acaaaatata gctccttgct tctctaagac agaagctata
    21901 caagaaaact gtggaaatat atatttcaga aaaacaaaaa acagccaccg gatgaagtat
    21961 ccgctgactg ttcaatttga cggctcaagt tttactgttt gagatgcaag atactcaacc
    22021 aggcaatggg cacataatta aagaaaaaac tgattctttg gcaaaaacct attttattca
    22081 gtaatggcca agaatttata gaaggaattc ttgccagtcc ataaatcgcc gcaaaaagca
    22141 aactgatcac agacagtcca aggtataagt ggcttacgtc catttgccca ttttttcgat
    22201 agatcaagaa aagaagaact ggaaacaata aaaaaccact gtaacctaaa cctgatccga
    22261 tattatggac ccatgtcgag acattccacg aagattcatg ggtattgata ctgaacaaac
    22321 ctgtaaagat acaatcacct ataccataga acgcgatggc tgctgtaact agggtagcta
    22381 acacacgaga tgtactaaca aatgcagcat acaatgcagg caatgacagg atgaaaaaga
    22441 tccctgaaac cacagaccaa ataagaaacg cttttcttac aggactggaa acctctccaa
    22501 atacactgat cacgctcttc atttgatcta attcaggata atacaatcct aatacgtacg
    22561 gagtcaaaaa atcactcagc acgcctaata ataaaaagaa aaaaccaaat ttacgtaaaa
    22621 aatccatgat acgcctccaa taacaattac ctatgctttt aattgttatt ataaacgact
    22681 ttatcttgat ttaacagaaa cctctattta cacgaacatc tatataactc attaaaaact
    22741 cgaatcattt taaaaatact aaatatctaa cgttcaacgt tcacttaatc catctaaaca
    22801 atctttcaat ttgatttttt ccccacttct tttacgcgcg cctagctctc ctattgcagt
    22861 aaacaacaga tcacttgacg aatttaaggc agtttccatt gaatcctgga taacaccgat
    22921 aataaatcca ataccgacga tttgcatcgc gacatcatta gagataccaa acaaactaca
    22981 tgctaatgga atcaataaca gtgaaccgcc cgcaacacct gaagctccac aagcagctaa
    23041 agcagacaaa atacataaaa ggactttcaa aacaaaaggt gcttcaatcc ctaaagaatg
    23101 tacagcagtc aacgtcatca aggaaatggt aatcgcggca ccgcccatat tgatcgtagc
    23161 acctaacggg atcgatatgg aataagactc ttttgtcaat ttcaatcttt cagccaacat
    23221 catgtttatc gggatatttg cggcagaact tcgggtaaag aaagcgggaa gtgcgctttc
    23281 tttcaaacaa aagaaaatca atggataggg attttcttta ataaacaaaa acgtgaggaa
    23341 tgggtacaca actaatgcga caaacagcat cgtaccgatc aaaacaagta aaagctgcag
    23401 atactctgcc aatccagcga ttccagtcgt agcaattgat tgataaacca atcctaaaat
    23461 ccctatcgga gcaaatgcaa taatcacttg tacgacttgt gacaacgcta tagatatctg
    23521 atccaccaac tcttttgtcg attctgaacg tgctcgaaaa gctagtccca acaatacagc
    23581 ccagaataag acgccaaggt aattcgcatc aataagagat tgaacaggat tttgtgtgac
    23641 attcgtcaac atagtagata aaacactttc caaactttgc ggtgcttttt cagatactgt
    23701 ttcttgtaaa gtaataggaa cagtaaaaat tgaacttaca ataactgcta caagtgcaga
    23761 aagcaatgtt cccactaaat ataagatcaa aataggcttc acgaatgtct tattcccaat
    23821 tttatgcttg gcaattgacg ccatcgtcaa gaaaaaaacc agaataggtg cgattgcctt
    23881 caagcaactg acaaacaacg ttcccaatat tgaaataaat gtccatgtgg ggaaaaagat
    23941 accaaacaat attccgacta aaataccaac aacaatacgt tgaactaacg acatatccaa
    24001 aaacttctct aacaaatgac caccctttca caattttaac aatcattgta tcattttatg
    24061 caaagaaact aaaggtataa ttttctaaaa attctaacaa tacaaacaac catttcttgc
    24121 taattttgta ctttttctgt ctacacgttg ttttttgaga tattctaagt taggatgtta
    24181 ttaatgggca acttatcagg aaacgttttc ttatgttttt gtccaatttt tttgtgaggt
    24241 gtttattctt gaaaaaaata aagaaggaga gttatggctt cctgacaatc tttgtcgctt
    24301 gtataatgat ggttttacag cgtatattga tttcagcagt cactattcct acatatgcaa
    24361 caaaccctgg tcgattctac atctatacat ttttacaaac ttccgttgtt attggtgcaa
    24421 atttgatccc actttacatg ggttaccaat atcaaaaaat cgcccgttta aaagttttac
    24481 attatttaag tcgttttgcc tttatattcc taatagccac actagtttgc aatatctttt
    24541 tttacgtaca agctggcagc ttgaatattc gtgattattg gcttatccta acacctatca
    24601 gtcagaatta ctttacttat gctgtttctt gtactttgct tttttgtagt attccttata
    24661 cagttggatt tttaaataaa ttatcgaaaa caacaattaa atatctacta ttatttttaa
    24721 caattggatt agttggctgc tctactctat ttaataaaga cccgtttgca ttacagaatg
    24781 gacagagtat tttatggatt ttttatttat ttttaatggg ttatggtttg aaagaatgga
    24841 actggaaaga aaaggtccgc tataagcttc ctcaagtatt tatcagcttg attgttttat
    24901 tcggtttgat tattttgatg acacaaattt cactcatcat cagaggaaat acagatacag
    24961 cgctgaggtt ttctaaacca tattctctct tcgcaatgta ttatactttc tcttcgtttt
    25021 tatttttaga aatgctatct gataaattag gtataaaagg aagaggaaat tttcttgctg
    25081 tatttttgat tgtcactcaa gttatatgta atttcccttt agtttcttat cgcgtatcta
    25141 catttatgaa acgtgaattt ccaaactcag ggaaggcatg gctgataaat atcgggcagt
    25201 tttttatttg atatttatta gcagtgtccc tcctagttat ctttttattt tatatacaaa
    25261 aaatccgttt cattcatcgt ttcatcgaat acttcgcttt tgattcgatt gaacaattaa
    25321 ctcagcgttg gaaaaaaaga attcattggc tttttgttca taaaagttat tttttagttg
    25381 cagcattttt ttactgcttt accttcgtgc agattttcgt tttagaacca aaagaaggtt
    25441 ggaaagaaac tatccaggtt gccttaaaaa tatttactca gcgacaagca cccatgatcc
    25501 tgacaatcat cattattatg ggatttttct tcttactatt gttgatttcc aatcgttttt
    25561 ggtacgcctt gactgctacc ttgatcatta acttactgct cactatttcg acagtaatca
    25621 aaatggaaat gcgggaagag cctgtctttc cttcagattt aaaaatgctg accggattgt
    25681 cagaactatt atcgatggtt agcccagtct tattaatcgt gggcggattg atccttctcc
    25741 tattattgat tactagtatt attgtacaac gtagactaca aaaacagtat tccttgaaga
    25801 ttcattggaa acgtcgattt atcagtatcg ctgtattatt aggatgtttt tcgggtgtct
    25861 ttttcattaa tcacaaaaat tcgccttctt tcttgctgtt taatttattt aaagtaaaca
    25921 agactttctt taaccagcaa ggcgccgtaa aagataacgg accagtcatt caattcctaa
    25981 acaatatcga cattaaaatc atggaagaac ctgctggcta ttcgaaagaa aaaattaatg
    26041 gaataatgaa aaagtacaac acaaaggcag atgaaatcaa ccaaacaaga agtgactggt
    26101 taaacaatca gacgatcgtt ctcaatttaa gtgaaagctt cagtgatcct gcacgtgtcc
    26161 ctaatctgac agtacctaca gaccctattc cttacatcaa aagcattata acagaaggaa
    26221 caggaggatt gatgttatcc gtcggttatg gtggcggaac agctaacatg gaatgggaag
    26281 gactgacagg cttgagtatc agtaacctgt ctgcttcgtt agttacacct tatacccagc
    26341 tagtggagcg tcagaaaata agtccaaata ttaccgatct attcgatgaa aaaatagcca
    26401 tccatccttt tacagctgct ctttatcgca gaaaacaagt gtttgataaa tttggttttg
    26461 acaggttcta ttatttagac agtccggata aattaactta tacggataaa atagaacaaa
    26521 gcccacgcat atcggatgat tcagcataca aagaaacctt gaaagcttta caaagcaata
    26581 cggaaacgtc acaattcatc caattatcca ctatgcaaaa ccatatgcct tatgatgatt
    26641 actatagtga attgaactac actgctgaag gaactgctgt cattcctaat agaagaaaag
    26701 aattacaaac ttatatgcaa ggccttcact ataccgatac agcagtaaaa aaattcatcc
    26761 aagaaatcga caaaatcgat aaaccgatta cctttgtttt ttatggtgac catttacccg
    26821 caatctatag tgggaatgat tcgaaaaaat atggattaga acagcaccag acagattatt
    26881 tcatttataa caacaagtat agtcgtgaac aagcagctac gttaaataaa gaagttgttg
    26941 ctcctaataa ctttggtgct atggctcttg agcatgcgaa tatcaaagta actccttact
    27001 atgcgttgtt gacagaagta tctaatcatc tacctgcatt aacaattgat cctacagaaa
    27061 gcttgtctaa tcgttttaat ggaaaacagg tctttgttac agaggatagt caaattatcc
    27121 aagaaaaaaa tttaactgag aaacaaaaag aaattctaga agattatcgt ctgatacaat
    27181 atgatcttgt tgcaggtaac cagtacagtg caacatgggc gacacaaaaa ataaaagact
    27241 aacaaataaa ctgagcaagg ggttgtgata aaagacatgt cacaacccct tgtttatctc
    27301 attaatttgc ttgtccataa acgatttgat taaagccatt tttcaattca ttattcgctg
    27361 tctcgttgaa gtaaagataa ttatcgggat tcccctgatt ttgttgtgtc accaatactt
    27421 ttggctgatt ttgctgaaac ccgaatagat agacgtgctt tttcagatat ggttctgtct
    27481 ctgcatcgga atagacggct gtgatttgat agtctgcttg acctgtccca ctttctgacc
    27541 attctgcaga aacaggcact ccaccaatag ccatcttcca ctccccgttc aaaatagctt
    27601 gagggacttt aagaccatat aagtccacac tcatttgatt cgtataactt ttgtattgtt
    27661 gtcccattgt ttggccccat ttggtcataa atgcctctaa ttgtgcggat ttgtctgcat
    27721 tccacagtgt ttgttcaact tgttcgctgc tttctgaaga ggaagatgaa gcctctacag
    27781 aagaacttga agtagatgtt tgagttgttt cagtcgtttt ggaagactct gacgaagtag
    27841 aggtatctgc tgtttctgta cttgctgcag ttgacttgct ctctgtgcta gttgttgtag
    27901 tagttgcaac tgttgctttc gctgtttctt ttgttgattc ttttttcgat tcattcccgt
    27961 tggtacaacc agctaacaaa aaagctagac caattgttac taaaatcctt tttttcaatt
    28021 aaaaaactcc tttcgattac tcccacttct ttattctatc aaataagcaa tgtgatttta
    28081 ttgatttttc gtattttctc atcaatcttt caaatcttct aaaaactgag aagcacccgc
    28141 tgatgttgtc tgtaatacat cagcgggtgc ttcttttatt ttaatcttcg tgattttcca
    28201 atttacctca cgctttatat ttgtatttga agaaacatag acttttagtc atcatcgtct
    28261 tcctcatcca aatcttgtcg gacagttttt ccttggagtc tttgtacttg gtagatgatt
    28321 tctttattga aacttaaaag ttcaacaagc aattgttgca agccatattt ttcttctttt
    28381 tcagctaatt tgattgcccg tgtgataaag acattttgga aatcaaaatc ttttgcaaag
    28441 ttttccaaca ttgttgaggc tggtaaatat ttgttttgac catgttcgta aatttttcca
    28501 taacgcatga cttcttctaa agtagacgga acgatttcat tttcaatcac taactcatga
    28561 gagattttat tgaaccatct cgcttcttga tcagcatatt ctgaaaaaag ggtacttaat
    28621 tcttgcgaag atgtacccaa aacatacaat tgtgcttgga tcaatttcat ttcttctaat
    28681 ttaaaatttg ctaatacatg ccccaccatg gcgccagcag ttggtttatg atggtcgata
    28741 tctgcttgtt ttgtttcttg aacaaatttt tcttccacta tattcattga tttacgcctc
    28801 ttttactttt gctgattgga cttcataacc taatttttcc acaactgcag ctaattgttc
    28861 agggtggact tcatctggat caaagtttgc tttgattttg ctcgcattaa acaaaacttt
    28921 cgtattgctt accccaggct gttgagaaac cgcatgttcg attttttgca tacagcttgg
    28981 acaagctagt gtatctaatt gcataataat ttttttcatc gtagttcttc cttccttttt
    29041 cttgatagtc ttattctaga ttacagcttc cataaaaaaa ttgattctta tcaagtttta
    29101 cgatttttct tctttgcttt cgtgtccaaa atacaataaa cgcatcgcgt taaagataac
    29161 gatcaagata ctagcttcgt gaacgaacat cccactagcc atgtaaataa acccggcaaa
    29221 taagccgttg agtaaaaata agacgacacc aatggcaatc actacatttt cacgggtatt
    29281 taaaacagtt ttcttagcca agcggtaggc atgaaccaac gattcaaaat tagattgcat
    29341 caagacaacg tcactcgttt cgatagcaac atccgttcct gatcccatag caatcccgat
    29401 atcagccgca gccaaagagg gagaatcatt gattccatct ccaataaaag caacgcgcaa
    29461 tccttgttct tggtattttt tcacaaattg aaccttttcg tctggtaata attcagcatg
    29521 gacttcatca atgcctaaca tatcagctac ataatcagca gtcatctgat tatcacctgt
    29581 cagcatcact aactgttttg ctcctgcttg tttcaactta gctaattgtt cagcgacttc
    29641 tggtcgaatc acatctgaca cacctatgat ttgagtgatt tttttgtcga ttgctacaat
    29701 aatgaccgag ctgcctgttt gttgtaattg atgtaaatct tgtacttgct ttggtgtgag
    29761 agaaagtttg tgagcggcaa tcaatttggt gttgcccgca caaactgtat gaccatcaat
    29821 ctcagctgtg attccttgtc ccttgattgt ctgattatct ttgactgcta attgttgaaa
    29881 atcaatgtct ttctcctgag cataatggat cacggcgcgc cccagaggat gatcggataa
    29941 cgtttctaat cgagccacta aagctaagct gtccccttca tcctctgtat agttcttgat
    30001 agtggataca gcagtagttc ctttggtcaa tgtaccagtt ttatcaaaga caaaagtatc
    30061 gattttggaa aaagtatcca ttgcttcacc gcctttgact aagatgcccc gtttcgctcc
    30121 attaccgatt cctgctacat tcgaaacagg cgcaccaatg actaaagcgc caggacaacc
    30181 aagaaccaaa acagtgattg ctaaacgaaa atcacgacta atcagaccaa caaccaacgc
    30241 aataagtaaa acggttggag tataatactg agaaaatcga tcaatgaatt tttccgcatg
    30301 ggatttggta tcttgtgctt cttctactaa ttcgataatt ttggcaaagg tcgtatcatc
    30361 tccgacctta gttgcttcaa tcgtcacgta gccatctgct aaaatcgttc cagagaagac
    30421 ttcatctcca ctgattttga cgacttgtcg tgattcacca gtcactgcgg cttcatccgc
    30481 ataccctttc ccgtcaatga ctcgtccgtc cacaggaata gctccaccag tttttactaa
    30541 gacgatatcg ccttcttcca cgtcatcgat ttccacttct tcagtcgttc catcttcttg
    30601 gacaacacta gcagtagatg gggccatctc ggtcaaagcc ttgattgact cccgcgtttt
    30661 ggtcaatgtt ttttgttcca gatagctacc aaacaaaaac aagaatgtaa cgatagctga
    30721 ctccttgtat tctccgatca taaaggcacc aagcacagca atagtaacga gtaaatcaat
    30781 tgagataact ttgactttca ctgcctgata cgcatggatc ataataggta tgaccgcaat
    30841 aatagaagct atggctagag caatgttata gaacaagaca aaaccagtaa attttccgat
    30901 aaaaccaaaa ataatcaaca cagctgataa ggcggtgata tgatttcgat gctcttggat
    30961 ccacttttgt aatttcatct ttctccctct tttcctttca ttcttacgag actaagtata
    31021 gatacttatc catcataaaa aattgaccac tatcaagttt agtcaaaaag gcggttttct
    31081 tttggtagaa tacactacaa aaaatagtta gcaagaagac tcactaaaag tgatcttgcc
    31141 aactattgtc gtttctatcg aaattttcat tgaatgattt tcctgtacag catacatcgg
    31201 agatctattt ctttatctaa gatttttatc ttgctaattt tttcctttag tctttcaaat
    31261 ccacgtcgtt catagaattg atacgtacag ccactatctg taaaaaggaa tatttctttt
    31321 cctttctctc ttttttcaaa ttctgttagc aacttggtac cgatgccttt tgttttcaaa
    31381 tcaggatttg cagctaaaaa aatgatctct ccatctggcg aatgcttctt taaatatgct
    31441 gcaagcattt gttcatttgc tgtctcgtaa gctcctttac tttctttagc aaatatattt
    31501 tgtaaaaaat cgaatatctt aacatatatt tgctgtccaa aagaacgatg tttctttttt
    31561 tctcctttta tctccgccag caaaacacct gccaactcgt cctctgcata caaagcaaga
    31621 atttgagttg ctcgattcaa ctccatatac aaaaaatatc ttccgtaaag attcagcaga
    31681 agttcatttt ccatatatct ctcaaagttc attccttcaa cagcaaaata gatagctttt
    31741 ttgtaatctt tcttttctaa gggtttaaaa gttagtttca tcatttcgcc tcctcaactt
    31801 caatgattgt gttatactta cattataata gacacagtgt ctactatcta taatcaatca
    31861 tctataagtt tgtcgattaa gcgacagctt tgctttgata tgtcggataa ggagataggt
    31921 aaatgaaaaa gacagataga cgcgtcaaaa aaacagaaaa agctttggct gaaacactat
    31981 ccacattatt agtaaataaa aagattcaag caatcactat acgcgaacta acagaaacgg
    32041 cagatgtaca tcgttcgact ttttatacac attataagga tatctatgat ctatatgat
//
LOCUS       DAYYBH010000025        31957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000025 DAYYBH010000000
VERSION     DAYYBH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.025"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1016..1357)
                     /locus_tag="ACHAUG_001329"
     CDS             complement(1016..1357)
                     /locus_tag="ACHAUG_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8480430.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="ACHAUG_001330"
     CDS             complement(1357..1587)
                     /locus_tag="ACHAUG_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8480431.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1874..2212
                     /locus_tag="ACHAUG_001331"
     CDS             1874..2212
                     /locus_tag="ACHAUG_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480432.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2417..2979)
                     /locus_tag="ACHAUG_001332"
                     /pseudo
     CDS             complement(2417..2979)
                     /locus_tag="ACHAUG_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3711..4292)
                     /locus_tag="ACHAUG_001333"
     CDS             complement(3711..4292)
                     /locus_tag="ACHAUG_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8480433.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4475..5101)
                     /locus_tag="ACHAUG_001334"
     CDS             complement(4475..5101)
                     /locus_tag="ACHAUG_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8480434.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5123..6454)
                     /locus_tag="ACHAUG_001335"
     CDS             complement(5123..6454)
                     /locus_tag="ACHAUG_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8480435.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6609..7010)
                     /locus_tag="ACHAUG_001336"
     CDS             complement(6609..7010)
                     /locus_tag="ACHAUG_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8480436.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7096..7302)
                     /locus_tag="ACHAUG_001337"
     CDS             complement(7096..7302)
                     /locus_tag="ACHAUG_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480437.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7397..8899)
                     /locus_tag="ACHAUG_001338"
     CDS             complement(7397..8899)
                     /locus_tag="ACHAUG_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8480438.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACHAUG_001339"
     CDS             complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACHAUG_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8480439.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9470..10087)
                     /locus_tag="ACHAUG_001340"
     CDS             complement(9470..10087)
                     /locus_tag="ACHAUG_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8480440.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10308..11681
                     /locus_tag="ACHAUG_001341"
     CDS             10308..11681
                     /locus_tag="ACHAUG_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8480441.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11756..12868
                     /locus_tag="ACHAUG_001342"
     CDS             11756..12868
                     /locus_tag="ACHAUG_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8480442.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12940..14577)
                     /locus_tag="ACHAUG_001343"
     CDS             complement(12940..14577)
                     /locus_tag="ACHAUG_001343"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8480443.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14745..15353)
                     /locus_tag="ACHAUG_001344"
     CDS             complement(14745..15353)
                     /locus_tag="ACHAUG_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8480444.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15737..16888)
                     /gene="queG"
                     /locus_tag="ACHAUG_001345"
     CDS             complement(15737..16888)
                     /gene="queG"
                     /locus_tag="ACHAUG_001345"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8480445.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16907..17659)
                     /locus_tag="ACHAUG_001346"
     CDS             complement(16907..17659)
                     /locus_tag="ACHAUG_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480446.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17800..18750)
                     /locus_tag="ACHAUG_001347"
     CDS             complement(17800..18750)
                     /locus_tag="ACHAUG_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8480447.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18751..19590)
                     /locus_tag="ACHAUG_001348"
     CDS             complement(18751..19590)
                     /locus_tag="ACHAUG_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8480448.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19587..20333)
                     /locus_tag="ACHAUG_001349"
     CDS             complement(19587..20333)
                     /locus_tag="ACHAUG_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8480449.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20571..21941
                     /locus_tag="ACHAUG_001350"
     CDS             20571..21941
                     /locus_tag="ACHAUG_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8480450.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21996..22892)
                     /locus_tag="ACHAUG_001351"
     CDS             complement(21996..22892)
                     /locus_tag="ACHAUG_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8480451.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(23006..23176)
                     /locus_tag="ACHAUG_001352"
     CDS             complement(23006..23176)
                     /locus_tag="ACHAUG_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480452.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(23173..23850)
                     /locus_tag="ACHAUG_001353"
     CDS             complement(23173..23850)
                     /locus_tag="ACHAUG_001353"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8480453.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23941..24846)
                     /locus_tag="ACHAUG_001354"
     CDS             complement(23941..24846)
                     /locus_tag="ACHAUG_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8480454.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            25021..26475
                     /locus_tag="ACHAUG_001355"
     CDS             25021..26475
                     /locus_tag="ACHAUG_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8480455.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            26491..27522
                     /locus_tag="ACHAUG_001356"
     CDS             26491..27522
                     /locus_tag="ACHAUG_001356"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8480456.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27529..28716
                     /locus_tag="ACHAUG_001357"
     CDS             27529..28716
                     /locus_tag="ACHAUG_001357"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8480457.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28872..30254
                     /locus_tag="ACHAUG_001358"
     CDS             28872..30254
                     /locus_tag="ACHAUG_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8480458.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30291..30611
                     /locus_tag="ACHAUG_001359"
     CDS             30291..30611
                     /locus_tag="ACHAUG_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480459.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(30811..31479)
                     /locus_tag="ACHAUG_001360"
     CDS             complement(30811..31479)
                     /locus_tag="ACHAUG_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8480460.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(31668..>31957)
                     /locus_tag="ACHAUG_001361"
     CDS             complement(31668..>31957)
                     /locus_tag="ACHAUG_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8480461.1"
                     /translation="DQGWAYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLK
                     QEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQSLVQFRKTTVTVV"
BASE COUNT         9053 a         6470 c         5749 g        10685 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     1621 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     1681 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     1741 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
     1801 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
     1861 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
     1921 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
     1981 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
     2041 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
     2101 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
     2161 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
     2221 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
     2281 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
     2341 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
     2401 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
     2461 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
     2521 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
     2581 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
     2641 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
     2701 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     2761 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     2821 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     2881 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     2941 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     3001 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     3061 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     3121 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     3181 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
     3241 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
     3301 taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
     3361 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
     3421 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
     3481 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
     3541 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
     3601 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
     3661 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
     3721 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
     3781 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
     3841 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
     3901 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
     3961 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
     4021 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
     4081 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
     4141 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
     4201 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
     4261 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
     4321 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
     4381 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
     4441 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
     4501 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
     4561 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca
     4621 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
     4681 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
     4741 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
     4801 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
     4861 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
     4921 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
     4981 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
     5041 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
     5101 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
     5161 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
     5221 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
     5281 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
     5341 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
     5401 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
     5461 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
     5521 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
     5581 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
     5641 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
     5701 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
     5761 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
     5821 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
     5881 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
     5941 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
     6001 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
     6061 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
     6121 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
     6181 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
     6241 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
     6301 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
     6361 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
     6421 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
     6481 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
     6541 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
     6601 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
     6661 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
     6721 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
     6781 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
     6841 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
     6901 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
     6961 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
     7021 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
     7081 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
     7141 caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt
     7201 ttctacataa tcttttatat gattgatttt aagatacata tataccagaa agttgacccc
     7261 ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg
     7321 gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc
     7381 tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata
     7441 attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta
     7501 atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct
     7561 cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt
     7621 cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc
     7681 tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt
     7741 catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga
     7801 catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga
     7861 tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc
     7921 gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat
     7981 gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg
     8041 tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg
     8101 cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag
     8161 cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc
     8221 ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt
     8281 tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta
     8341 gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat
     8401 aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct
     8461 gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt
     8521 ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg
     8581 agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga
     8641 tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc
     8701 ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct
     8761 tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca
     8821 agtttacttc atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca
     8881 gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt
     8941 ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc
     9001 ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc
     9061 ggcaccattt cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac
     9121 ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa
     9181 taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat
     9241 gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag
     9301 ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg
     9361 tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc
     9421 ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt
     9481 ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga
     9541 tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt
     9601 ctaatttctc aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac
     9661 aaagaccttc ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc
     9721 tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca
     9781 tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca
     9841 gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat
     9901 tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt
     9961 taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa
    10021 cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt
    10081 tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg
    10141 agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa
    10201 ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata
    10261 atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa
    10321 aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat
    10381 tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga
    10441 ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc
    10501 tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc
    10561 ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg
    10621 gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct
    10681 tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg
    10741 ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga
    10801 atcatatctt cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg
    10861 ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa
    10921 tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct
    10981 ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag
    11041 tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga
    11101 ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc
    11161 ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag
    11221 cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga
    11281 agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca
    11341 taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg
    11401 gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag
    11461 gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat
    11521 tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta
    11581 ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg
    11641 ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg
    11701 ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga
    11761 aaaaattgcc attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac
    11821 atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc
    11881 tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa
    11941 agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta
    12001 cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct
    12061 tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga
    12121 acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg
    12181 tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca
    12241 aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa
    12301 gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa
    12361 gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt
    12421 agcatttcag acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga
    12481 agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga
    12541 tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta agagtacaga
    12601 acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac
    12661 ccgtgcttat acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt
    12721 actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct
    12781 attagccaac ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc
    12841 cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc
    12901 gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa
    12961 attctccaaa ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat
    13021 gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa
    13081 agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt
    13141 gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat
    13201 ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa
    13261 caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa
    13321 tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg
    13381 ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat
    13441 tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg
    13501 atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat
    13561 atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca
    13621 agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta
    13681 agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg
    13741 caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga
    13801 accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat
    13861 cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt
    13921 cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc
    13981 atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat
    14041 ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt
    14101 cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag
    14161 caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa
    14221 ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc
    14281 cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc
    14341 attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt
    14401 taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc
    14461 aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga
    14521 taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc
    14581 tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat
    14641 ttgtactgct gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact
    14701 tgaataaaca agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt
    14761 gctaaccaga atgttttagc agcatcccat gaaccataac gagatgttac atattgttct
    14821 gctacacgtt cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga
    14881 taacggccga tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg
    14941 atccattctt ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct
    15001 acttcaactg tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta
    15061 gatgctgtat tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg
    15121 attgttaatt gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca
    15181 attgcatcga tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca
    15241 cctgatttta ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca
    15301 gtagcaccag cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac
    15361 tccttttttt ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt
    15421 tcataggtaa aagaaacatg acatttaatg acaaaataac gaattattta atatttgtaa
    15481 catttaaaca aatatttttt tgatacaaaa ccataaaagc cttgttgtat aagcaaagta
    15541 atcgtttttc tatgtttttt tatttttgaa tgaaaaacgc agattctgta acattcactg
    15601 caataatcca taaaaaaaag caaaaaaatt tttgattttt atcaaattct cttaaaaaaa
    15661 tgtaaaaaat acaaaaaaac cagtaattgt tacaacaatt actggacaaa aactttgaat
    15721 aatgttaact atttttttat tttaactttc tccgcaataa tttctgggca tgttccaatt
    15781 cgtttataac gacttcttct gtttctttag ctaatatttc atctaataca tcaagccatt
    15841 tttccggttc ttttgttcct agacgtccaa tagcccaagc tgctgttcct ctgatgacag
    15901 ggcggacatc tgttaaacat tctgaaatct caggcaatgc cgttcgatca cctagattag
    15961 ctaaagcaat caatgcgttt ctctgcaaag gtttcttccc acgccatgaa ccagctaaat
    16021 gcccaaattg ctgtttgaag tctttattag acatcgaaag cattggcttt aattttggat
    16081 atatttcgtc tacttttggt tccatctctt catgaaaatg aaaatctttt ccttgattgt
    16141 aagggcagac taactggcag atatcacatc cataaatgac atttcgcatt tttttacgat
    16201 attcttccgg catcatccct tttgtctgag tttgataaga caaacatcgc tgggcattca
    16261 ttcttccgtc accaagcaat gcttttgtcg gacaagcagt cacgcagcgc atacagtcac
    16321 cgcaaccaaa ttctcctggt ttatcaggct caaatacaat attagtaacg atctcaccaa
    16381 gatagacaaa tgaaccgaac tcttcagtga tcaacaatcc gtttcgtcca ataaatccta
    16441 aaccagctcg ctgtgctaca gcgacatcga tcagttctcc agtatctact tgcggagcta
    16501 gacgccattc ctcttctttt tgccattttt ctgctcttgt ttggatgaat tcgatcaatc
    16561 ggttcaaacg gtctctgagt atatcatggt aatcgatccc ccaagaagca cgagcaaact
    16621 gcccgcgttt ttcatcccta ggcacttctt catggatcct agtaggatac gcaagtgcaa
    16681 tggaaatgat cgatttcggc tgatcaaatg tcatttctgg ataaagacgc tcatcgatat
    16741 ttggatgttc aaagccagaa gtatggccag ctgctttttg ttcttccaac gattctttta
    16801 atgaatcaaa aggttccgcc gtggtgaacc cgattttgtc aattcctaat cgtttgcttt
    16861 cttgtatgat ttcttctttt aatgtcatgg tcttcctcgc ttgctgttaa cggctgtata
    16921 acccataata tagtttttct tttttcagct ggacaaactg gatctgctga tcggcaatct
    16981 tcttttcttc catccatttt tttcttgcag caaatacttc ccaataattt gttgaaatta
    17041 attttctttt gttatctaat gcattaaaaa aattcaatga aactggtttt gtacttaaca
    17101 gttcatggag ccttacctct tcccatggcg aaaactcaga cagatcgcgg gaatacaata
    17161 cttcttcata gagatttttt tgctgtgtgt acgtgaatgt ttgcgggatt ttcccaggcc
    17221 tagcaatttc tactagacga ttataaaaca tattcattaa tttgctgttg gcaataaatt
    17281 gacttttata ataggccgac tggatctttt tgattactcc ttctgctttg atgactgaaa
    17341 ataatgcagg ccatgctttg acggttattt cgtcgttttg tttaactaat acgccaggtg
    17401 tgaagccgac tggagaaagg aacacaaaat aaccgatacc ggtattttca tctaaaggct
    17461 gaaattcatc aaacaaccat ttgcctatgt acagctcttc atctggttct gttgtctcat
    17521 tttgatttgc tatgaataat ttagctaatt ctgcttcaag aaattgaagg tcttgatcct
    17581 tgtcggtatt ttcctgctgg ctgatcaatc gaaagacttc ttctttaatc gtatatttat
    17641 ctttacgtgt aaaattcata gccacacctt ccttccttac tctaactata acggaatttc
    17701 ttcaaaaaag gtatctgtac gacgaaaaac agcataattt tttttcatat ctcttataaa
    17761 aaatgcaagg tctgctcctc aagctagacc ttgcactttt cagctttctt ttgtttgtga
    17821 caaaccttca tggatcgttt caatattcca tttcatcatc tgatagtaag aatctccttg
    17881 ttctccttct tttgctatgg aatcagtgaa cagtttatca tagattttca aacccgtttc
    17941 ttttgctacc cgttccatgc ttcttgagtc tacactggtt tccacgaata aaaccggtac
    18001 ttcttttgct cttatctgat caatgatcgc tttcatttga tcaggcgtac cttgactttc
    18061 tgtattgatc tcccatatat aagctgctgg caaatcatat gctcgtgaaa aatacttgaa
    18121 tgccccttca cttgttacta ataatttttt gttctcttcg atagaagcaa aactttcctt
    18181 cgcttgtgta tctaattctt ttagtttttc cacatatgct tctgcatttt ttttatagag
    18241 tgtcgcattt tctgcatctt tttcagagaa tatacgtgcg atttcctcca catattttat
    18301 tccgttagat aggtctagcc aagcatgggg atctgctttt gtatgttctt caccgctagt
    18361 taaataaaga ggttctacat ttttgctaac tgcaaaataa tcttttcctt cttctttttt
    18421 agccgtttcc atcaagttat cgaaccagct gttacctgtt tcaagattca aaccgttgta
    18481 taaaataaca tctgcatcgc ttgccttttt gatgtcttct ggtagtactt catattcatg
    18541 cggatctgtt ccgactggta cgatactgtg gatatctatt tgatctgttc cgacctcttt
    18601 tgccatgtcc gccaagatcg aattggtagc cacgactgac aacttttctt tacttccttc
    18661 atccgcttgg tcagaagcct gctttccgca accggctaat accaagaaac taactgccaa
    18721 gattaaaaag agtgattttt ttgttttcat ctatctgctt cctttctgaa ataaaattcc
    18781 ttttgttgga gaaaataaaa aagcaaggat aaaaaatgcg gtacatgcta acacgatcgt
    18841 agctcctgat ggccaattgt aactataact gaaaaacaca ccgacaactg cacttagcat
    18901 accgattcct gcagctgtaa tgatcatttt cagtaaatgg ttcgttagca agtaagccgt
    18961 ggcggctggt gtgatcaaca tggcaatcac caagattgtt cctactgtct gtaaactgac
    19021 tacagccact aatgtcaaaa agaacatcaa aagataatgg atcagcgatg tgttcagccc
    19081 ataagctttt gccattgtcg gatcgaatga tgtgatcttc aattctttat aaaagaaaaa
    19141 gacaaatatc aagacgattc cactaacgag tgctgtcagc aacaaatcgc tttccctaac
    19201 agctaagaca ttcccaaata aaatatgata caaatctgtc gcactctggg catatgaaat
    19261 caagataatc cctaatgcaa aaaaggagct aaagacaatt ccaatcgctg tatcattttt
    19321 caatcggctc cgctgagtca cgaaaccaat cactgctgct gttagcatcc caaaagcagt
    19381 ggctccaaag atgtaactaa aaccaaacat ataagaaatg gctacacctg gaagaactgc
    19441 gtgagagata gcgtctccca tcaaagacat ccctcgtaaa ataataaaag aaccgatgac
    19501 tccagaagca agtcctacaa gcatggaagt gaccaaagcg tattgcagga attgataatt
    19561 cattaatcca tcaataaagg attggatcat tttttctctc ctccttttac taaatgtctg
    19621 atgatctcac cataggccaa ttgaaggttc ttcgatgtaa agacttcttg tactggaccg
    19681 gaagcgatca gctgtttatt taaaagaata acctcatcga aatattcttc tactttatga
    19741 agatcatgat ggacgatcaa aatcgttttt cctgaattct tcaattcctg caagatgtca
    19801 aagatctttc gttcacttaa cgcatcaatc cctacgaatg gttcatctaa aaagatccat
    19861 tctgctcctt gggctagagc tcttgcaata aaaactctct gaagctgtcc acccgatagt
    19921 tcgctgatct gtcttttggc atattcttcc atccctactt ttttcaaagc ttgtcttgca
    19981 cgttcttttt cttttttccc aggtctttgt ccaattcgta aagatggata tgttcctaaa
    20041 agtacaacgc ctaatacatc aattggaaag gaaagatcca attcacttcg ctgttccacg
    20101 taggctattt ttttacggac tgagtttacc gactgtccag aaaaggtgac tttaccagta
    20161 gtagctggta tcaactctag caaagatttc ataaatgttg attttcctgc accgtttggt
    20221 ccgataatac cggtaatttt ttgtggattt atcgtcaagt tgatagtttt caaaacagtt
    20281 ttcccttgat aagcggctga aaggtcttga attacaatgg ccgtacgttc caaatcttac
    20341 actccttttt aggtttacct aactttttat ttaaatatag tttaactaaa atggaaagca
    20401 ttttcaagta gaatgatgaa aaagtttttt tcgaattttc ctcgcatttt atgaaaattt
    20461 tctgtagaat cacatcaaga gttcaataag gctaacctaa taatgaaaaa catgtgttga
    20521 aacgtttgat tattttttct tgttgactca acaacgaaag gagtttttgt atggaagaag
    20581 aaaagttaac tcgaagtctt agtgcccgtc atatccaaat gatcgcacta ggtggaacga
    20641 tcggtgtcgg tctgtttatg ggtgcctcct caaccatacg ttggacaggg ccctcagtga
    20701 tgggagccta tgcgattgca ggattctttt tgtatttgat tatgcgtgct ttaggcgaaa
    20761 tgctttacgt tgatccttcg actggttctt ttgcaaatta cgcgagcgag tacatccatc
    20821 ctgtggctgg ctacttgacc gcttggagta atatttttca atatattgtc gtaggaataa
    20881 gtgaagtgat tgctgtagga tcttatatga attattggtg gcctgatttg ccggcaatta
    20941 ttcccggaat cattgtcgtc ttgtttctta tgctggcaaa tcttatttct gtcaaggcgt
    21001 ttggcgaatt agaattttgg ttttcgatca tcaaagtcat aacgatcatt ttgatgatca
    21061 ttgcaggtct tggggtaatc ttatttggat ttggtaatca tggtcaggca gtagggattt
    21121 caaatctttg gaaaaatggt ggattcttca ctggaggcgt caaaggattt ttctttgcat
    21181 tatcgatcgt tgttgcttcc tatcaaggaa tcgaactgat cggaatgact gctggtgaag
    21241 cagagaatcc taaaaagacg atcattgaag ctgtccagtc cactatcggt cgaatcttga
    21301 ttttttatat tggtgctatt tttatcattg tgagtatcta tccgtggaat caattgagtg
    21361 agattggttc accttttgta caaacttttt ctaaagtcgg cattacattt gctgcaggac
    21421 tgattaattt tgtcgtgatt acagcggcat tatcaggttg caattcaggg attttcagcg
    21481 caagtcgtat ggtctataca cttgccataa atggaaagat gtctaaaaaa tttctgaaac
    21541 tatctcgtca cggtgcgcct ttttatcctg tcgtagcaat ctcaggtgga atcttaattg
    21601 gtctgatctt aaattatctt ctaccaatgt tcatcaacca gtcagaaaat attttcgttt
    21661 tcgtatacag ctcaagtatt ttgcctggta tggttccttg ggtagtgatt ttgatcagcg
    21721 agattcgctt caaaaagaag cacgcagata aaatgcatga tcatcctttt aaaatgccac
    21781 tatctcctta tacgaattat gtgacacttg cttttttagc tatcgtattg attttcatgt
    21841 ttatcaatcc tgaaacgaga atctcattga ttattggact cgttttcttg ctttacatga
    21901 cgatccatta ctttgtacgc gaaaaaagaa ccggaaaata aaaaaagaac gaagcttcac
    21961 cttagtggaa gcttcgttct tttttattgc ttttatcaat tcaattttag cgtctcaccg
    22021 ggagtcagga aataattctc catgctcatc ccattcagcg acaagatcgt ttctactggt
    22081 acgccagttc gttcggctat ttgatttgga ccttctcctt ccaaaactac ggttgtcgca
    22141 gcctgtgaag tcgcgctgtt ggtattagtt tcagaggaat ctgttgtgtc ttgtgttgtt
    22201 cgagcttgca tttcagaagc ttctaccgac tctgctgtct cagaatcaga agatgttgtg
    22261 tcttgtgtgc ttgttgactc tgcttgaaga gaggcgctag tctgcaaata aatatcggaa
    22321 atcttagcga catgattcgt ataattagcc gataattgat tgatcaaagt atctaagtct
    22381 acaccgtctt tattttctgc tgcctgatac gctgcacgta attttctgat cccttcgtcg
    22441 gagattgctt gaagctcacg tgctttttgg ttagcaactg ctgataaacc agctattccc
    22501 tcttgatttc cctgaatttc tgtctggtat tcttcaatca atgcaggcgt cttttcttcg
    22561 atctttttcg aataatcatt tacgattgct tgaagttgct ctggcaaacc atttgctgtt
    22621 ttttcttcag aagtttgtgt ctcttttgtt ttagttgatg actcacttgt ttgtttcgtc
    22681 tctgttttct ttgtcgttgt ttccgttatg gtcgtacttt tcgcttctga cgaatttttt
    22741 ccatgaaaat atagacccgc aactcctcct gctatgatca aaaacacggc aactgctgcc
    22801 ccaatcatga tcatccgctt acgagctgcc tgtttttgct gtcttcttcg ggtcatccga
    22861 acattttctc cccactcatt ttcatcatac atttttttca ccaccaattt ttatgtataa
    22921 tagcgttatc aaacgcttca tattcttatt gtagcaaata attgtcgctg tgcacatttt
    22981 tatctttgtt atttataaga aaatttcacc agttgagatg attccaagat tgtccgtcga
    23041 tttctccttg cagctgcatt gttcgttcgt ctatctcagc catcagtttc tttatttcat
    23101 ataagagcgc acggtcttga tattctgatt tttcgcttaa ttcatctaat tgttggatca
    23161 gccaatcatt tgtcataatg aagttccttt catttcttgc agtgtaaagg tcaagtcttc
    23221 gatctgctct tttgcctcag tcagtgcttc tacgatatat gcttgttttt cagattcttc
    23281 ttcctgcatt ttctgatttt cctgtactct ttgaataaac cagtcaatcc gctgagtcag
    23341 cgatggtttg ccttccaatg agagatactg ctgataagca attagcttct cccaacgttt
    23401 ttcctgggat aaatacataa attgtgctaa aacgaatggc ggaagatagg tcatatggaa
    23461 tttatttgcg attcgctgat acggcctcaa aaattcggaa atcgtatatt cttcttttcc
    23521 tccagcttgg tattcttttt ccgccacacc tgttgtcaat acgattccta gttctttccc
    23581 ttttaataca tgtgctgctc gttccaagac attatcttgc caaattttta aatgagcagg
    23641 agaactatac cagtaaaacg gaaactggaa aatgatccga tcatgctggg ccaagagctg
    23701 tagttcagtc tctatatcta tttttccgtc tggataactt gattccaaat gatgccaagt
    23761 agctggtaaa ccagcagaag cttctttgaa aaattgctga tttaaagatt cttcgatcgt
    23821 tggatggcta atgatgataa gtgtacgcat cccttttcct cctttttagt cttcgcttta
    23881 cagtgtagca taaccgccac aagtttcttt tgccgatgct tgtcatttta accagactaa
    23941 tcaggttgtc caagaaactg cggtttgttt ttccaactgt tttttcaagg tatccaaagc
    24001 aatattctta aaattttcgt ttttcatacg tttacgatag ttcgtagccg tttctccggt
    24061 actctttttg aacattcggc taaatacgac tagactattg aaccctactt gataggcaat
    24121 actttccacg gattcatccg tcagagaaag aagaaacgcc cctcgattga tccgtacccc
    24181 gatcaaatat tccttgatac tggtacccat atgatttttg aatactcgcg acaaatggct
    24241 ccgattgacc gctaaatgct gggcgatttc ctcaacagac aatttttcag cataatgtgc
    24301 ctcgatgtat ttgactgttt caacagctaa agaggaatga accttttgta caccaccgct
    24361 gacctttccg ccatctttta aaaggactga taaaaaacgg taggttaatg ctgttaactg
    24421 taattcattt tccaaggttt cttccgtatc tttcatgcat tctaaaataa tgtcgatata
    24481 tttttgtatg tcagaagaga ccatcgtgta ttggtcttct ctgaaaagcg actgccgaat
    24541 gatttcttct gcagcactcc caccaaatga aagccagcaa tacatccacg gatcttctcc
    24601 atctgataag taaaacgtcg agtctcctgg acgaatcaag aacagatcac cttttttcaa
    24661 ttggaattgt tgatttttca catgataaga tcctttgcct tccatcacga catgcaggat
    24721 gtaatcattg cgaatagcag gaccaaaaga atgaaacggc atcgttttag agaaaccaca
    24781 aaaatcaaaa tacaattctt tttctacttg ttgatgttcc ttttttaaaa aaatcgattg
    24841 ctccacgtct atccctcctt actcctttag tataatgcaa caaaaagtta aataaaagtc
    24901 acagaaatat attaaaaagg aagcgatttc ttgttaagtt taagtcatta aaagatcttt
    24961 gttattcgtt gtaaccggtt tacttagtat tatatattga acaggagagg aatacagatt
    25021 atgaaaagca aaatgatcca acgtgtgtcc tacgctttag gcgcttttgg acacgacaca
    25081 ttctatgtta cattaagcac ttattttatg gtattcgtga caagccagat gttcgcagga
    25141 acagataagg caacgaatgc aaaaatgatt gctacagtta caagcttagt tgtaggtatc
    25201 cgcttagttg aaattatttt tgatcctttg attggtggca tcatcgataa tactcgtaca
    25261 aaatacggta aattcaaacc atggttagtt atcggcggcg ttgttagttc cgtactcttg
    25321 gtagcaatct ttaccaattt ctttggctta gctactcata atacgacttt atttatcata
    25381 ttatttgcaa tcgttttcat cttattggat agtttttatt catttaagga tatcgctttt
    25441 tggtcaatga ttccagcttt gagtacggaa acaaaggaac gtgaaaaatt tgctacgttt
    25501 gctcgtttcg gctcttctct aggtgcaaat ggtactactc tagtcgttgt ccctatcgtt
    25561 actttcttta cctatcttgt tactcataaa catattgaag gcgcttcagg ctggttctgg
    25621 ttcggtgtga ttgctgctgt tatttcaggc ggtactgcga ttatcactgc cttgggcaca
    25681 aaagaagtcg atactgcgat ccgtcagcaa aatgaaaaag ctgggatcaa agatgtgttc
    25741 aaagcagttg ctcaaaatga tcagttgatg tggcttgccc ttgcttatat cgcttatgcg
    25801 attgccaatg cagcaacaac tgccgtatta ttctacttct tcaaatatgt acttggccaa
    25861 ccaactgaat tttggatcgt tggtctagtt gctgccttac ttggtttagt agctgttcca
    25921 ctattcccaa tgcttacacg cgtcatctct cgtcgctacg tttaccttgg agggatcgca
    25981 atgatgattt cttcttactt tttctttacg atcgcaggtt caagtttgcc aatcgttatc
    26041 atcgggctag tattgttcta cttccctcaa caattgattt tcttatccgc tttgatgacg
    26101 atcacagact ctgttgaata tggtcaatgg aaaaatggta tccgtaacga agctgttact
    26161 ctatcaattc gccctttact agataaaatc gctggtgctt tgtctaacgg gatcgtaggg
    26221 tttgtcgcta tcgcttctgg gatgactggt aatgctactg ctgctgatat cactgctcat
    26281 ggcgtaacga ctttcaaagc ttatgcattc tatgctcctg ctgctttgat gattttctcc
    26341 gctattattt ttgcttggaa gattacattg acagagaaaa aacatgcaca aatcgttgag
    26401 aaattggaaa acaaattggc tccagctgat acaatggaag atgaacttca cgatgcagta
    26461 gatccttccc attaagaaag gatcttgaaa atgaagatta cagaaaaaga ttttggccaa
    26521 ggttatcact tgataacctt ggagaacaaa aacaagctag cactttctat ctctgattta
    26581 ggtgctagaa tcgtcagttt aaaatcaaat gatcgagaat tagttttggg atttgacacc
    26641 gctgaagaat atatcgaaaa agatccgtat atcggtgctt ctatcggtcg caccgcaggg
    26701 agaatcgaaa atggccgctt ttctctcaat ggaaaaactt accaattagc cacagatcca
    26761 aaaactggac ataatctgca tggtggtgct cctggatttg aattaaaaaa atggtcttat
    26821 gtcattttga atggcgaaaa tgaagcttct gtcattttta ctactacaag tcccgacgga
    26881 gaacatggtt ttccaggtac aatggatgtg gaaatccgct atactttgac aaaagataat
    26941 atctggcgag tgacctctcg cggcaccagt gaccaagata ctttattcaa tccgacaaat
    27001 catgtttact tcaacttgac gggagatgct tctcagtcaa tcgatcaaca tgagctttgg
    27061 ctcaatagcg aagcttatgc tcctttgcga acagatagta ttcctatagg ggtaaaagaa
    27121 aatgctgcag gaagtgcttt cgattttcaa atacctaaaa aattagcgtc cgtttttgct
    27181 agtgatttgg atcaaaaaaa tcttgttgac ggaatcgatc atccattttt cttaaaagaa
    27241 accggcttgg gtaaagaagc cgctcgcttg actagccccg ataaaagaat ccaagtagat
    27301 attgctacag atgcttctag tgtggttatt ttcactgcaa atttcggcac agaaacacca
    27361 gaaatgcgca accgaaaact ggcacatcac ggtggcatca catttgagac tcaaacagct
    27421 ccaggtgcag agcgcttctc agcatttggt tcgattcatt taaaagccgg atcagtattt
    27481 gaaacagtaa cagaattcaa aatcaaaaca agaaaggaat aactcatcat ggaaatcaaa
    27541 agaagcttaa acacaaaatt tgccgaaaca ttcggaccta aaaaaactac acaatacttc
    27601 tcaccaggac gcatcaattt gattggtgaa cacacagatt acaatggagg ccatgtcttc
    27661 cccgcttcca tcacttatgg aacgcaaggt gtagcagcac ctcgagacga taataaaata
    27721 ttagtttatt ccacaaactt tgacgatatc ggtgtcatta gttttacttt agatgagcta
    27781 gaatatgata aaaaagcaaa ctgggcaaat tacgtaaaag gaatggtcct taaattaaaa
    27841 gaagctggac actcaatcga tcatgggttt gagttattag tggaaggaac aatccctaat
    27901 ggtgcaggac tttccagtag cgcttcatta gaattattag taggtgttgt attagaagat
    27961 ctgtttgatt taaaaacaga tcgcttggaa ctagtccaaa caggtaaaaa agtagaaaat
    28021 gaattcatcg gtgtcaattc aggtatcatg gatcagtttg ccatcggctt cggtgagcta
    28081 gatcatgcaa tcttattaga tacaaatacg ctgaaatacg aaatggttcc agtgaaacta
    28141 gatggttatg caatcgtcat catgaatacg aataagcggc gggaattggc agattcaaaa
    28201 tacaatgaac gccgtagcga atgcgaagaa gccttggcac gtttgcaaaa aactttgcct
    28261 atctcttctt taggagactt agatgaagaa gaatttgaat caactattga ccaaatcggc
    28321 gatgatacat tgatacgcag agctcgccat gccgtttctg aaaatcagcg gactttgaag
    28381 gcaaaagctg aattagaagc tgggaactta gaagcatttg gtcaactgtt aaatgattct
    28441 caccactctc ttcgctatga ttatgaagta actgggatcg aattagatac actagtagat
    28501 gctgctcaaa aacaagaagg tgtcttaggt gcacgaatga caggagctgg atttggtggc
    28561 tgtgcgattg ctcttgtcaa agaagaaaat atacctgcct tcaaaaacaa tgtctatgat
    28621 gaatacatga aagttgtcgg ctatgctccg gaattttatg ttgcacacat tggaaatggg
    28681 acaacaaaga tcgatccaga agctgttcat gtttgataaa aaaatcaacg atctcagcta
    28741 aaaacaaata gataaaaata acctctcagc aatttccaaa tgtgagcctt ttgctcgtat
    28801 ttgaaaattg ctgtttttta ttgacacgga aagagaacgt gtgttctaat tgtgttgaag
    28861 aggtgattgt tatgtgggcg aatcaaaatc cggtatttgt ttatgaaaaa gaaccttcaa
    28921 gagatatttt atgtattgac tgcaaatctt tttacgcctc cgtagagtgc gttgaaaagg
    28981 atcttgatcc ccttacaact aaattagtag tcatgtccta tccgtccgat tcgacagaaa
    29041 cacggggtag cggattaatc ttggcttcca gtcctgcagc aaaaaaagct tacggcatca
    29101 ccaatatcag ccgtgccaga gatcttccct tcccctatcc aagtgattta tatattgttg
    29161 ccccaaggat ggcctattat atggaaaaga acagagaaat caattctatc tataaaaaat
    29221 atgcagacga agcgaaccat catgtctatt ccgtcgatga aagcttcctt gatgtaacaa
    29281 atagcatggc actctttggt aaaacaaatg cgtacgacat ggctaaactg atccataaag
    29341 atgtgtttga gcagaccgga atctacacga ctataggaat tggcgataac ccgctacttg
    29401 ctaaactagc cttagacaat ggctcaaaag acggaaagca aatgtttgct gaatggcgtt
    29461 atgaagatgt accagaaaag ttatggaaaa tctcagatat cactgacttt tggggaatcg
    29521 gcaaacgaac agcaaaacgt ctgaaacaaa tgggtataca ttctgtttat gatttagctc
    29581 acgcggatta ttatcacttg aaaagcaagc taggggtcat cggtactcag ctttatgccc
    29641 atgcttgggg catcgatcga agttttttgg gacaagaata tacaccaaaa tcaaaaagta
    29701 tcggaaatag tcaaatccta gatagagatt atacaagacg agaagaaatc gagatcgtta
    29761 tcaaagaaat ggccgaccaa gtcggcactc gcttgagacg atctgcagca aaaacacaag
    29821 tagtcagctt atgggtagga tactctttag gctatcttga tccaaatggc aagaccgggt
    29881 ttcaccagca aatgaaaatc gaagcgacaa atgctagcag agatctagca gatgctttat
    29941 tgaccatttt ccaccagcat taccactacc aggatattcg gcgtgtaggc gtgaactgtt
    30001 ccgacctcgt atacgcgaca ggtttacagc ttaacctttt tgaagatcct gaaaagcaga
    30061 taaatgaagc gaaagttgat tttgttgtcg atacgattag aaaaaaatac ggctttcaat
    30121 caatcatcca tgcgcatagt ctcttgcaag gaggacgcgc catcgcacgt agttctctcg
    30181 ttggcgggca tgcaggtggt atgagtggat tagaaggtgg aaaagatgag acggacagca
    30241 aaaaaattca ttgactataa cgagtatcga gatcggccat ttggattaaa atgggggact
    30301 gcttttgcga tggaagaact agtcaacggt atcaaaaaaa tgaagaagaa gcacgcaaac
    30361 agcctcttcc taaacaacaa atgacattgc aagaaatcga tagtatactg acagaagcct
    30421 ttttatctcg taaagcagta atcattcagc tcaatttaaa agatgaattt ggaagactct
    30481 tggatgaggt cgaagggcac ttcattggtg aagcctatga agattacttt atgattgacc
    30541 atcagccgat attgtgggaa gacgtacggt atattgagtt aaaggaaaca aaaaaatggt
    30601 ttgtgacatg agaggttgtg acagaagtga tcaacttcaa aaaataagaa aggatttttc
    30661 gaaaattgct tcttaaattt ttgtggaatt ttggcttatt tttcgaagaa gcaacttctg
    30721 gaacaacgta tattagatat cagaggttgt gaaaaagtcg cttagttttg agtaacaagc
    30781 caaggcgctg atttcacaac ctctgtctat ctatccatct attcggtaag attcatgcat
    30841 gacgtcttct ttatttagta tgtagagccc tttttcttct gaccattcaa cataaatcag
    30901 ctgttttaca tctgcatagc cttctttctt caagttctcc ataagccatg cttcagtcaa
    30961 ttgattggac tgaagcactt tatgctgaat ttgcccttca tctacaagca ggatcgataa
    31021 agctttgtct tcatctgatt ttttcaatac actcatttga ccggcattct ctaggataac
    31081 atgtttggca ttttcaagaa aaaagcattc ttgctgtctt aagattgctc gcaactgctc
    31141 catctcgata tggttatttg tcaactctgt catgttgatg acgccgtctt tgattagaac
    31201 agatggctcg cctttgaccc aacgattgac cttttcattt ttttggacga tacgttcgat
    31261 accataaatc attactgccc aaagcgcaat ggcaaatagg acatgcccta catggacatt
    31321 gtcatcatag gttgattctt ctaagatccc acctaaaaca agcgtataga ctaaatcaaa
    31381 cggtgtgatt tctgacatgg atttcttccc aagcaaacgt accactaata ataaaccgat
    31441 caacccgaac accagtttca ttgtaattga aaaaatcatt ttttctcccc ctaactatct
    31501 gttaattaaa ttatatatgc tttctcacga acacgcatgg aatatacaag gaagaaatgt
    31561 atccttttac taaaaaaagg actgtgacac tgtagtgacc cccaaaagtt agactttttt
    31621 ggagtggaga aatccactcc ttttttttat gctctaaaat gatattttta cacaaccgtt
    31681 acggttgttt tacgaaattg gacgagactt tgccagttta atttctgttt tatccgttca
    31741 ttattgtagt aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa
    31801 actttcccgt gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta
    31861 tctaagcagt tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt
    31921 ctataagctt tcatttgata cgcccaacct tgatctg
//
LOCUS       DAYYBH010000026        31349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000026 DAYYBH010000000
VERSION     DAYYBH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.026"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACHAUG_001362"
     CDS             complement(451..1761)
                     /locus_tag="ACHAUG_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8480462.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="ACHAUG_001363"
     CDS             complement(1773..3131)
                     /locus_tag="ACHAUG_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8480463.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3333..4901)
                     /locus_tag="ACHAUG_001364"
     CDS             complement(3333..4901)
                     /locus_tag="ACHAUG_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8480464.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4905..5315)
                     /locus_tag="ACHAUG_001365"
     CDS             complement(4905..5315)
                     /locus_tag="ACHAUG_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8480465.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5312..6235)
                     /locus_tag="ACHAUG_001366"
     CDS             complement(5312..6235)
                     /locus_tag="ACHAUG_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8480466.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6232..7110)
                     /locus_tag="ACHAUG_001367"
     CDS             complement(6232..7110)
                     /locus_tag="ACHAUG_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8480467.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7097..8434)
                     /locus_tag="ACHAUG_001368"
     CDS             complement(7097..8434)
                     /locus_tag="ACHAUG_001368"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8480468.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8437..9540)
                     /locus_tag="ACHAUG_001369"
     CDS             complement(8437..9540)
                     /locus_tag="ACHAUG_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8480469.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9614..11122)
                     /locus_tag="ACHAUG_001370"
     CDS             complement(9614..11122)
                     /locus_tag="ACHAUG_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8480470.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11088..11780)
                     /locus_tag="ACHAUG_001371"
     CDS             complement(11088..11780)
                     /locus_tag="ACHAUG_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8480471.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11802..12776)
                     /locus_tag="ACHAUG_001372"
     CDS             complement(11802..12776)
                     /locus_tag="ACHAUG_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480472.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13076..13948
                     /locus_tag="ACHAUG_001373"
     CDS             13076..13948
                     /locus_tag="ACHAUG_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480473.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14406..16604)
                     /locus_tag="ACHAUG_001374"
     CDS             complement(14406..16604)
                     /locus_tag="ACHAUG_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480474.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16836..17828
                     /locus_tag="ACHAUG_001375"
     CDS             16836..17828
                     /locus_tag="ACHAUG_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8480475.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17888..19309
                     /locus_tag="ACHAUG_001376"
     CDS             17888..19309
                     /locus_tag="ACHAUG_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8480476.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19302..20801
                     /locus_tag="ACHAUG_001377"
     CDS             19302..20801
                     /locus_tag="ACHAUG_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8480477.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20840..22234
                     /gene="uxaC"
                     /locus_tag="ACHAUG_001378"
     CDS             20840..22234
                     /gene="uxaC"
                     /locus_tag="ACHAUG_001378"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8480478.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22393..23076
                     /locus_tag="ACHAUG_001379"
     CDS             22393..23076
                     /locus_tag="ACHAUG_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8480479.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23078..23914
                     /locus_tag="ACHAUG_001380"
     CDS             23078..23914
                     /locus_tag="ACHAUG_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8480480.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23925..25679
                     /locus_tag="ACHAUG_001381"
     CDS             23925..25679
                     /locus_tag="ACHAUG_001381"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480481.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25955..26296)
                     /locus_tag="ACHAUG_001382"
     CDS             complement(25955..26296)
                     /locus_tag="ACHAUG_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8480482.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26364..28535)
                     /locus_tag="ACHAUG_001383"
     CDS             complement(26364..28535)
                     /locus_tag="ACHAUG_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8480483.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28746..29603
                     /locus_tag="ACHAUG_001384"
     CDS             28746..29603
                     /locus_tag="ACHAUG_001384"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8480484.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29674..30474)
                     /locus_tag="ACHAUG_001385"
     CDS             complement(29674..30474)
                     /locus_tag="ACHAUG_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8480485.1"
                     /translation="MTIKKKLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGID
                     KTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNS
                     CIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAA
                     GPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLL
                     QKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(30471..>31349)
                     /locus_tag="ACHAUG_001386"
     CDS             complement(30471..>31349)
                     /locus_tag="ACHAUG_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8480486.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPL
                     EVFLSYVSIDDLSNLI"
BASE COUNT         9367 a         6443 c         5541 g         9998 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact agtgaaaata
     3301 agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc atcatacttg
     3361 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
     3421 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
     3481 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
     3541 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
     3601 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
     3661 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
     3721 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
     3781 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
     3841 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
     3901 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
     3961 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
     4021 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
     4081 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
     4141 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
     4201 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
     4261 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
     4321 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     4381 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     4441 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     4501 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     4561 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     4621 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     4681 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     4741 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     4801 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     4861 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     4921 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     4981 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     5041 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     5101 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     5161 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     5221 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     5281 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     5341 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     5401 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     5461 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     5521 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     5581 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     5641 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     5701 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     5761 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     5821 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     5881 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     5941 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     6001 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     6061 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     6121 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     6181 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     6241 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     6301 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     6361 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     6421 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     6481 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     6541 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     6601 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     6661 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     6721 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     6781 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     6841 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     6901 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     6961 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     7021 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     7081 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     7141 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     7201 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     7261 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     7321 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     7381 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     7441 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     7501 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     7561 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     7621 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     7681 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     7741 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     7801 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     7861 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     7921 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac
     7981 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     8041 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     8101 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     8161 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     8221 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     8281 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     8341 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     8401 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     8461 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     8521 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     8581 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     8641 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     8701 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     8761 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     8821 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     8881 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     8941 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     9001 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     9061 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     9121 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     9181 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     9241 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     9301 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     9361 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     9421 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     9481 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     9541 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     9601 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     9661 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     9721 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     9781 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     9841 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     9901 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     9961 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
    10021 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
    10081 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
    10141 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
    10201 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
    10261 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
    10321 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
    10381 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
    10441 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
    10501 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
    10561 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
    10621 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
    10681 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
    10741 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
    10801 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
    10861 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
    10921 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
    10981 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
    11041 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
    11101 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
    11161 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
    11221 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
    11281 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
    11341 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
    11401 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
    11461 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
    11521 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
    11581 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
    11641 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
    11701 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
    11761 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
    11821 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
    11881 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
    11941 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
    12001 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
    12061 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
    12121 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
    12181 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
    12241 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
    12301 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
    12361 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
    12421 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
    12481 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
    12541 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
    12601 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
    12661 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
    12721 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
    12781 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
    12841 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
    12901 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
    12961 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
    13021 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
    13081 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
    13141 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
    13201 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
    13261 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
    13321 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    13381 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    13441 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    13501 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    13561 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    13621 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    13681 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    13741 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    13801 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    13861 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    13921 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    13981 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    14041 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    14101 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    14161 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    14221 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    14281 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    14341 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    14401 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14461 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14521 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14581 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14641 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14701 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14761 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14821 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14881 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14941 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    15001 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    15061 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    15121 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15181 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15241 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15301 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15361 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15421 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15481 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15541 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15601 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15661 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15721 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    15781 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15841 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15901 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15961 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    16021 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    16081 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    16141 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt
    16201 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16261 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16321 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16381 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16441 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16501 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16561 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16621 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16681 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16741 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16801 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta
    16861 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16921 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16981 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    17041 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    17101 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    17161 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17221 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17281 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17341 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17401 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17461 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17521 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17581 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17641 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17701 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17761 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17821 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17881 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17941 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    18001 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    18061 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    18121 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18181 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18241 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18301 acagagcctc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18361 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18421 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18481 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18541 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18601 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18661 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18721 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18781 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18841 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18901 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18961 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    19021 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    19081 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    19141 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19201 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19261 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19321 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19381 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19441 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19501 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19561 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19621 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19681 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19741 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19801 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19861 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19921 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19981 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    20041 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    20101 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    20161 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20221 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20281 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20341 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20401 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20461 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20521 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20581 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20641 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20701 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20761 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20821 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20881 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20941 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    21001 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    21061 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    21121 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21181 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21241 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21301 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21361 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21421 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21481 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21541 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21601 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    21661 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21721 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21781 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21841 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21901 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21961 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    22021 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    22081 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    22141 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22201 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22261 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22321 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    22381 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    22441 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    22501 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    22561 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    22621 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    22681 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    22741 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    22801 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    22861 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    22921 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    22981 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    23041 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    23101 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    23161 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    23221 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    23281 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    23341 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    23401 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    23461 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    23521 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    23581 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    23641 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    23701 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    23761 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    23821 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    23881 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    23941 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    24001 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    24061 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    24121 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    24181 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    24241 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    24301 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    24361 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    24421 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    24481 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    24541 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    24601 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    24661 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    24721 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    24781 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    24841 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    24901 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    24961 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    25021 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    25081 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    25141 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    25201 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    25261 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    25321 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    25381 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    25441 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    25501 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    25561 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    25621 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    25681 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    25741 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    25801 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    25861 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    25921 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    25981 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    26041 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    26101 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    26161 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    26221 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    26281 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    26341 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    26401 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    26461 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    26521 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    26581 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    26641 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    26701 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    26761 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    26821 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    26881 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    26941 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    27001 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    27061 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    27121 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    27181 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    27241 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    27301 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    27361 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    27421 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    27481 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    27541 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    27601 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    27661 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    27721 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    27781 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    27841 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    27901 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    27961 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    28021 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    28081 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    28141 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    28201 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    28261 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    28321 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    28381 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    28441 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    28501 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    28561 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    28621 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    28681 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    28741 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    28801 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    28861 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    28921 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    28981 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    29041 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    29101 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    29161 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    29221 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    29281 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    29341 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    29401 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    29461 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    29521 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    29581 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag cagataatga
    29641 taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa
    29701 cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga tatattcact
    29761 tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac ggattttttg
    29821 aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag gtgctaacgt
    29881 caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc aaacaggatg
    29941 atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc
    30001 taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt catctggatg
    30061 gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt
    30121 gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac tattttttcc
    30181 ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg ttgtccaagc
    30241 agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg cccacccttt
    30301 tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac caatcgggcc
    30361 gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa atacctgatc
    30421 caatgtttct tctggtgttg tggtagggat acttaatttt ttcttaattg tcaaattaag
    30481 ttagacagat catcaatact tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc
    30541 agtgattttc taggaatatt atttcgttta gatgcgacag attgaataaa aaattcatct
    30601 actgaattga aatccataga tttcaataaa ccatctctac gtaacaatcc gttagaattc
    30661 tcgtttaggc ctctttgaga cggcgttcct ggatcagcga aataaatggc aatatcattg
    30721 acattactga tctgtttcca atttgaaaat tcctttccac aatcaaaagt gatggatttg
    30781 aataggtttt tcggtacaga ttcaaaccat tgatttaatt ttttttcaat atcaatcgct
    30841 tgtctaccac acggtttcaa tgtgatgata acttttgata atcgttcaac taaggtaatt
    30901 acagcacttt tatgtttcag acctacgata gtgtcacctt caaggtgacc aaactcattt
    30961 gagaattggc tataatcctt ttcacgttca tgaatagagc ggcggaaagt ttgttttcca
    31021 cgtctttctt gatgtccatt cggtttacgc ttgcctttca tcggtaagtc agaagaatca
    31081 aatagcccct ttttgaacat acgataaatg gtacgagcag aacaactaat aggaaacgct
    31141 gcacgaccaa caatcacatc gggtgtccat ccttgaacaa cctttctttg aatatattct
    31201 gattgttcct cgggtaaaac aagcggacgt ctaccacagt ttgatttgtt tttcttatac
    31261 tgttgataat attctaaggc tgattttcct tgcttgaaga acagatatac tttatggatc
    31321 gtttgtcttg aacgattcaa gcaatgggc
//
LOCUS       DAYYBH010000027        28603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000027 DAYYBH010000000
VERSION     DAYYBH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.027"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACHAUG_001387"
     CDS             237..1877
                     /locus_tag="ACHAUG_001387"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8480487.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACHAUG_001388"
     CDS             2031..3353
                     /locus_tag="ACHAUG_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8480488.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACHAUG_001389"
     CDS             complement(3415..3846)
                     /locus_tag="ACHAUG_001389"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8480489.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACHAUG_001390"
     CDS             complement(3910..4746)
                     /locus_tag="ACHAUG_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8480490.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACHAUG_001391"
     CDS             complement(4936..5238)
                     /locus_tag="ACHAUG_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8480491.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACHAUG_001392"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACHAUG_001392"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8480492.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACHAUG_001393"
     CDS             complement(5692..5958)
                     /locus_tag="ACHAUG_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8480493.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACHAUG_001394"
     CDS             complement(6212..6937)
                     /locus_tag="ACHAUG_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8480494.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACHAUG_001395"
     tRNA            complement(7303..7373)
                     /locus_tag="ACHAUG_001395"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACHAUG_001396"
     CDS             7490..8242
                     /locus_tag="ACHAUG_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8480495.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACHAUG_001397"
     CDS             8258..8737
                     /locus_tag="ACHAUG_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8480496.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACHAUG_001398"
     CDS             complement(8988..10568)
                     /locus_tag="ACHAUG_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8480497.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACHAUG_001399"
     CDS             complement(10561..11304)
                     /locus_tag="ACHAUG_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480498.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="ACHAUG_001400"
     CDS             complement(11480..12256)
                     /locus_tag="ACHAUG_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8480499.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="ACHAUG_001401"
     CDS             complement(12504..13340)
                     /locus_tag="ACHAUG_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8480500.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="ACHAUG_001402"
     CDS             complement(13351..14280)
                     /locus_tag="ACHAUG_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8480501.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="ACHAUG_001403"
     CDS             14472..14825
                     /locus_tag="ACHAUG_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8480502.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="ACHAUG_001404"
     CDS             complement(14899..15555)
                     /locus_tag="ACHAUG_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8480503.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="ACHAUG_001405"
     CDS             15781..17715
                     /locus_tag="ACHAUG_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8480504.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="ACHAUG_001406"
     CDS             complement(17801..19024)
                     /locus_tag="ACHAUG_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8480505.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20889)
                     /locus_tag="ACHAUG_001407"
     CDS             complement(19018..20889)
                     /locus_tag="ACHAUG_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8480506.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="ACHAUG_001408"
     CDS             complement(20910..22028)
                     /locus_tag="ACHAUG_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8480507.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="ACHAUG_001409"
     CDS             complement(22111..22944)
                     /locus_tag="ACHAUG_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480508.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="ACHAUG_001410"
     CDS             complement(22937..23854)
                     /locus_tag="ACHAUG_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480509.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="ACHAUG_001411"
     CDS             complement(24081..25205)
                     /locus_tag="ACHAUG_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8480510.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="ACHAUG_001412"
     CDS             25388..26296
                     /locus_tag="ACHAUG_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8480511.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="ACHAUG_001413"
     CDS             26437..28050
                     /locus_tag="ACHAUG_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8480512.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5622 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta aca
//
LOCUS       DAYYBH010000028        28077 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000028 DAYYBH010000000
VERSION     DAYYBH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.028"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            855..2024
                     /locus_tag="ACHAUG_001414"
     CDS             855..2024
                     /locus_tag="ACHAUG_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8480513.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            2497..3051
                     /locus_tag="ACHAUG_001415"
     CDS             2497..3051
                     /locus_tag="ACHAUG_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8480514.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(3224..3661)
                     /locus_tag="ACHAUG_001416"
     CDS             complement(3224..3661)
                     /locus_tag="ACHAUG_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8480515.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3709..4656
                     /locus_tag="ACHAUG_001417"
                     /pseudo
     CDS             3709..4656
                     /locus_tag="ACHAUG_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4927..5362
                     /locus_tag="ACHAUG_001418"
                     /pseudo
     CDS             4927..5362
                     /locus_tag="ACHAUG_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            5378..6211
                     /locus_tag="ACHAUG_001419"
     CDS             5378..6211
                     /locus_tag="ACHAUG_001419"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8480516.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            6456..7481
                     /locus_tag="ACHAUG_001420"
     CDS             6456..7481
                     /locus_tag="ACHAUG_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8480517.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7619..7744
                     /locus_tag="ACHAUG_001421"
     CDS             7619..7744
                     /locus_tag="ACHAUG_001421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480518.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7769..8884
                     /locus_tag="ACHAUG_001422"
                     /pseudo
     CDS             7769..8884
                     /locus_tag="ACHAUG_001422"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8967..9863
                     /locus_tag="ACHAUG_001423"
                     /pseudo
     CDS             8967..9863
                     /locus_tag="ACHAUG_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9879..10703
                     /locus_tag="ACHAUG_001424"
     CDS             9879..10703
                     /locus_tag="ACHAUG_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480519.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10735..12045
                     /locus_tag="ACHAUG_001425"
     CDS             10735..12045
                     /locus_tag="ACHAUG_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8480520.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12193..13452
                     /locus_tag="ACHAUG_001426"
     CDS             12193..13452
                     /locus_tag="ACHAUG_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8480521.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            13462..14334
                     /locus_tag="ACHAUG_001427"
     CDS             13462..14334
                     /locus_tag="ACHAUG_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480522.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            14346..15176
                     /locus_tag="ACHAUG_001428"
     CDS             14346..15176
                     /locus_tag="ACHAUG_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480523.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            15216..17399
                     /locus_tag="ACHAUG_001429"
     CDS             15216..17399
                     /locus_tag="ACHAUG_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8480524.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            17460..18680
                     /locus_tag="ACHAUG_001430"
     CDS             17460..18680
                     /locus_tag="ACHAUG_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8480525.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18677..20137
                     /gene="gtfA"
                     /locus_tag="ACHAUG_001431"
     CDS             18677..20137
                     /gene="gtfA"
                     /locus_tag="ACHAUG_001431"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8480526.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            20214..21320
                     /locus_tag="ACHAUG_001432"
                     /pseudo
     CDS             20214..21320
                     /locus_tag="ACHAUG_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21642..21851
                     /locus_tag="ACHAUG_001433"
     CDS             21642..21851
                     /locus_tag="ACHAUG_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8480527.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21844..21993
                     /locus_tag="ACHAUG_001434"
     CDS             21844..21993
                     /locus_tag="ACHAUG_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8480528.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22147..22812
                     /locus_tag="ACHAUG_001435"
     CDS             22147..22812
                     /locus_tag="ACHAUG_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8480529.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23096..24181)
                     /gene="xerS"
                     /locus_tag="ACHAUG_001436"
     CDS             complement(23096..24181)
                     /gene="xerS"
                     /locus_tag="ACHAUG_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8480530.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            24515..24748
                     /locus_tag="ACHAUG_001437"
     CDS             24515..24748
                     /locus_tag="ACHAUG_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8480531.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24745..25152
                     /locus_tag="ACHAUG_001438"
     CDS             24745..25152
                     /locus_tag="ACHAUG_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8480532.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(26289..26807)
                     /locus_tag="ACHAUG_001439"
     CDS             complement(26289..26807)
                     /locus_tag="ACHAUG_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8480533.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27174..27422
                     /locus_tag="ACHAUG_001440"
     CDS             27174..27422
                     /locus_tag="ACHAUG_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480534.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            27925..>28077
                     /locus_tag="ACHAUG_001441"
     CDS             27925..>28077
                     /locus_tag="ACHAUG_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8480535.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         9628 a         4253 c         5144 g         9052 t
ORIGIN      
        1 taattttcta cgttttcctt ttcatatata ttaaatatta cggaaaatta tataaactaa
       61 tttagtgttt tgtttgtaat tcctaatttt gctccacttt tagataattt ttaggtctat
      121 ttaactcatc agacattcta tgctcatttt tctccaaatt tgtttcaact ctttcgtact
      181 taattttgcg tatttgaaaa ttcttctttt agagtacttt tattacgata ctagtatgat
      241 atatatctat agcaaacagt taaaatcttc ctaaaataag cgaatagcat tcctaaaacc
      301 tataaaattt ttagatgaag cgacaagtgg acaattaata aactacttga acatgaaatt
      361 atggatcacc tttcaaattt agaaaatgga attcttatac ttattgcgca acatttattg
      421 attgcaaaat tatgtgacaa aaaaaactag tagaagaaag aataatggct tgataataaa
      481 gtactaccta ttaaaggtag tactttattt aatttagtat aaattaacat actactatat
      541 tttaggtttc atattatata aattaataat atactaccat ttgttggtag tataatttaa
      601 aggtaataag ttattctttt attactatgt ttgggtttta tagcaaagaa agtttaataa
      661 gagtttggac ctagcaaaca atattaatga ggtcattaaa tcttattatc tttagtgata
      721 taacgaaata tctacacaga caaaatgata gcgtaaaata ttttgtgtaa atagatattt
      781 agggtagaaa aaaagaaagt ccattctgta aaattaaagt taccacaacc gaaattttat
      841 aggaggactt tctcatgccc aattttacta cagaaattat ggaaacacta atcaataaag
      901 gtgatttaga tgaattattt cgatgtcatt tagaattagc tgttaattca ttattgcagg
      961 ctgaattaac agcttttctt gattatgaaa aatatgatcg tgctgggttc aattcaggta
     1021 attcccgtaa tgggaattat tcacgttcat tcagaacaga gtatggagaa ttaaatttgg
     1081 taattcctag agatagaaat ggtaagtttt ctcaacaaac tttacctgct tataaaagaa
     1141 ccaatgactc tttagaaacc actattattc agctatttca aaaaggaatt actatgtctg
     1201 aaatctctga attgattgaa aaaatgtacg ggcatcacta tacgccacaa accatttcaa
     1261 atatgactaa aatagtttct gaagatatta ttgcctttaa agaaaggtcc ttagaatcca
     1321 gatactctgt catttttatg gatgctactc acattccttt aaaaagacaa actgtttcaa
     1381 aagaagcagt ttatattgta attggcattc gattagatgg aaccaaagag gttcttgggt
     1441 ttagtcttgc tccaactgaa tctgcttatg tttggaaaga gattcttcaa gacctaaaag
     1501 accgtggttt aaaagaggtt ttattagtcg taactgatgg tttaagtggc attaacgata
     1561 gtattcatag tgtttatcca aatgctcaat ttcaacaatg ttgtgtccat atatctagaa
     1621 atatcgctca taaggttcgt gttagtgatc gacaagaaat ctgtagcgat ttcaaattag
     1681 tttatcaagc aagttcaaaa gaagaagcaa ataatcaaat aaggtttatg atagataaat
     1741 ggaaaaagca gtatccacga gtggttaaat tgctcatgaa tcctgctata ttaacctttt
     1801 ataatttccc tccgtcaatc agaagaacta tttattcaac taatttgatt gaaggtttta
     1861 acaaacaatt aaagaaatat accaaaagaa aagagcaatt tcctaatgaa gaatctctgg
     1921 agagatttct agtatctcaa ttcaacaact ataatcaaaa gtttctatgc cgagttcata
     1981 aaggttttaa agaaatacat gatacattag aatcaatgtt ttaacttgat caacagaatg
     2041 ggcttttcca tttacacaaa attattgacg ctcccgacaa aattatgatc ttgcgtaaag
     2101 ttataaaata ttaataaaaa tattttatta atgaaaagct tggtagcacg gttattcata
     2161 tgctaattta tttcacttta tagtaagggg agttcttagt atattgttca aacttaaatt
     2221 tcatataatt taattatgta aattagctat tctaagaact tctacggata actgcccgtc
     2281 taaaatttaa ataagagctt agaatcttca tataagtaag gttaattttt ttgtacaaaa
     2341 aagtcggaat attctgagtt actgaaatga tataaaatca taaactaaat cccttgattt
     2401 tattgacttt caaaagttta tatatataag ttaatgatta tggtaaaata tatacataat
     2461 cattaactca gaatattttt aagaagggaa acaatagtga gctataatgt acaaccgtta
     2521 aggacaaatg aagagataca agattttcag ttttggctaa gaagaacaag taatccggaa
     2581 agagatagtt ttttattttt gtttggaatc aataatgggt tacgtatgtc agatattata
     2641 ggattaaaag ttggagatat acgaggcaaa agtaagccta ttattgttga acataaaaca
     2701 ggtaagcgaa aaccgatttt tatagataat ttgagagaag aaattttatt atatactgaa
     2761 ggtaaagaag aaaatgattg gttatttccg agtcggcaac aaggtcgaca tataacaagg
     2821 gatcgtgtct atcagatata tgcagatata gcagaaaaat taggacgaga tgatatagga
     2881 acacatacat taagaaaaac ttttgggtat cactactaca agaaaactag agacattgct
     2941 accttaatgt ttatttttaa tcatagctcg caagcaatta caaaaaggta cattggtata
     3001 actgaagatg aaataggagc atcacttcgt ggatttaaac taggtgtata atttaaaaat
     3061 ttttataaac agtaaagaca aagattaaaa gctattttta tctagaacga ttttatttca
     3121 taaagttcgc ggaataaaag ctggggttac tggtacttgg tataggttcg tgttatataa
     3181 actaagtaat taaataacat ttttaggttg gaaaaggatg aaattaaaaa aagagaatca
     3241 atacggtctc aaaaacgcaa acgctgcgga ataagaaaaa tactgataac tcaacgttta
     3301 gcagactatc taatagggat agtctgcttt tttcgttttt gaccatactt ttgaccttct
     3361 gcaaacttcc ttgcttttcc tctgtagaac tcatgaaatc tgcaaaacga attgccgttt
     3421 cctctgtctg ctttttcgcg atatgggtgt atatatcatc catagtggtt ttaatatctg
     3481 tatgtccaag tcgaacatac acttctttga cagacgcacc agattcaaag agcagactac
     3541 aatgggtatg acgaaaacca tgaagtgtga tatgttttaa gttattctct ttaatgatga
     3601 ttttcaaatt gtgatttaca tagtctaaat aaaggggctt attttcttct gatgaaaaca
     3661 aaagttgttc accatctttt ataggtttga ttctcatttt tagatattat gcccgcacgt
     3721 attcatcaca tttctattgt gaatcgcttt attcgaccga catttgactt ttatcataat
     3781 atactcggtt taaaattact aatgaaaaca attaatcaag acgatcatac catgtatcac
     3841 ctgttttttt cagataatca ccaccgtgtg ggaactgaac tgactttttt tgaagtacaa
     3901 gaagggaaca accagttttt tggaaccaat acgattgaac gaacgatact aaaagttcct
     3961 tccgaagcat cattgcattt ttgggaaata tattttgaaa cacaaggcgt ttgccattat
     4021 ggaatcgaat cattttctgg acgacctatc ttacgttttg aagggccaga tgcaacacga
     4081 ttagctttag ttcccttacg cgaatttgaa gatattgatg attatttccc gtatgtcaca
     4141 gaccagatcc ctacagagca tgccatttta ggaattgatg ccattcaact acgcgtacaa
     4201 tattccgatg cgactgaacg agagttattg tcggtaggct ggtagcacaa agaatacgtc
     4261 agtttctttt cgggtcatca acgagtgaca atcttagaaa atcaagacac tcagttctat
     4321 caagaagttc agattattca agatcgtgag aatccagttg ccattgaagg gattggtagc
     4381 gtccatcatg ttgcgtttgg cgtagaaaat aaatctgatt tacaacgcat cgacaaacaa
     4441 ttacaagagc gcaattttat aaattcagga ataaaagatc gtgaattttt tgtatcactt
     4501 tattatcgtg acccaaatca attattgatt gaaattgcta caggggaagg aaacctagat
     4561 gcaaaagcgt atgaaaatca aagtccacgc tttgaagaaa ttcctttatt tttaccgcag
     4621 tacttaaatt tcattcgtga acaagtggag caataattag ctaatcaaat ccatgacgaa
     4681 gagtaagagg ttgactcata agtcaaaaaa taaagaaaaa cgcatcaaat ccattctaaa
     4741 gaatgttgat ttgatgcggt tcctattttt ataaactatc attttcgact aataaaacta
     4801 cttatgagtc agcctctctt tttttcttga tttttatacc tctttctagg taaactaaac
     4861 atctgccaaa aataccgtgt ctcattgaaa cattctcaac tatttcaaaa taaaggggag
     4921 taccaaatga acgagtatat tgaaattatc cacgcaagag aaacaactta aaaatgtcag
     4981 tctcaaaatt cccaaaaata aaattagtat ttttaccagc gtatctggtt caggaaaatc
     5041 atcgattgtt tttgatacta ttacccaaga agctggtcgt caactgaatg acacctatga
     5101 tagttttacc cgtttatttt tacctaaata cagtcgtcca gaggttgatg agattgataa
     5161 tctttctacg gcgattgtgg tcaatcaaaa gaagattggt ggcagtgcgc gctcaacgtt
     5221 agggacgatt actaacatca atgcgttatt tcgtgtgttg ttttcatgtt ttgcaacacc
     5281 tagtttagga tatgcaaatg cttattcatt taatgatcct agtggaatgt gttctgtctg
     5341 tcagggaatc ggtaagacga ttgcattttt aataggattg ctttattttt cggctattga
     5401 taatgctgat tttgtatttt ctgatccatt taccgcagaa gttttgtctg gaattgagga
     5461 acaggcaaag aatcatggtt attttgtatt ggtgcataat gttaattcag cagaagatgt
     5521 taaaactatt cagagaaatt ggcgttttga ggggttcatt gttgttggtg tacgtgtaaa
     5581 agaatttttg gaattaaata acgtttttga tggtcctgtt gtttatattg atacgcatct
     5641 ttcaaaagag acattaagag aacttgaaca acaaatgaat cgtttatttg taaatacaaa
     5701 tgattaccaa gctagtacat tggcagtgga acatttgatt cattatggtc atgagaaaat
     5761 agcgtttctt tcttttgact acattgtagg tgaaccaagt gttattcaag aacgacatca
     5821 agcttatatc gatacattaa agaaatatca tttaaccttt aaatcgcatt ggatatataa
     5881 caactctgaa tttgagcgaa tttatcaaga attagctgaa tttactgcaa ttgttgttac
     5941 tggagattat ttagcggcta aattagtaaa atttttaaaa aataagaata aacgagtgat
     6001 ggaacaatta tcgattattg gatttgatga tattactttt gcagaattaa tggatcctgc
     6061 gttgacaaca atacgactaa atccaggtaa caagggggcg attgcttttc accaactgat
     6121 tggactggcc aaacaagaag aaataaaaaa tcaagtacta ctattagagg gagaattgat
     6181 ttctcgagac tctgttcaca aaatcaaata aaaaagctct gtgaaataag tatagttaac
     6241 aataaaatat ccgaacgata gagagaatga actgtgatct agcagttcct cgtcgcaaat
     6301 tcactagaat tagcattatt tactctaaat tgttcggata ttttttcttg tttttaatta
     6361 tgattacagt gcctattttt aacgaaattt gcttattttc aaaatgtatg attatatgtt
     6421 agtaaacact ttcaaatatg aaaagaggag ctattatggt aggaattagg gatgttgcaa
     6481 aacgggcggg tgtgtccatt gcaacagtat cgcgcgtgct aaatgaggat ccgacacttt
     6541 cagtaaccga tgaaaccaaa cgaaagattg cagaatcggt taatttttat cattatgtca
     6601 aaaaaagtcc cgttgcaaaa aatttaacaa atattgcatt aataacgaca gtttctgaag
     6661 tagatgagtt ggaagatccc tattttcgaa cgattcgcag agggattcaa gtcgaagcag
     6721 aacaaagcaa agtcaatttg aagaaaatta ttcgtttgtc agaaacaccg ttggagcaag
     6781 acgatttgct aaaatgtcaa ggtgtgttga ttattgggca ggtgctatct tctgtgattg
     6841 aagaagtatc agcaattaat tcgaatgtcg tagtgattga tgaccctagt attgaagctg
     6901 aagtagatgc agtttatact gatttaacaa aagcaacgaa tcagcattta gatcgattgt
     6961 ataaaaaagg acaccgaaac attgcgttta tcggaggagg acgtgttcgt ttagatagcc
     7021 aaggaaataa acacgtgtca gatgatgatt atcgaaaaat agcctatgag acgtggatga
     7081 aacaaaaagg attagaaaaa tatagtcaaa tttatttaga aggctggacc acacttgacg
     7141 gaatgcaggc agcagattta cttttgaaaa aacatgctaa agataagttg ccgactgcta
     7201 tcatggtagg aaatgatcct attgctgtgg gggtttatcg ttcacttcaa aaaaatggtc
     7261 tacatattcc agaagatatt tcgattgtga gttttgataa tattgaagtt gctgaatttt
     7321 taacacctgc attaaatacg gtgaatgtga atacagaaga aattggaaaa ctagcagttc
     7381 gcatggttaa tgaacgaata aaagaagtta gaaatgtttc gatcaaagtg attgtttcta
     7441 ataatattat tgttagagaa agtgaaaaaa atatcgatta aaaaagtaaa catccaaaaa
     7501 agggtgttta cttttttgtt tactaatatt tttagtaaaa aatctcgaca aaacattaag
     7561 taaacagtta tggtgttgtt aacaaaaaga aaacgcattc aaaacaggag gaattaagat
     7621 gaaacatttc aaaaaaatag ctttaacagg gatagctgtt ttaactggga cattactcgc
     7681 agcatgtggt gggggagata gtaattctaa agacgggcaa gtaactttaa gctatgcgag
     7741 ttgagatact gaccaagctc caggactaag aaaaatgtta gatgaatttg aaaaacaaaa
     7801 tcctgatatc aaagtagaaa tggagacaac gccttgggat caatattggg ttaaattaga
     7861 agctgcaaca acgggaggta atatgccaga tgtagtaacc atgcactcta gcgaatccta
     7921 taaatatatg tcacagaatg ctttgatggg acttgatgat gttatcaaag aaaatgatat
     7981 tgacatggga aactttactg aaggaattgc tgatttctat acgcttgaag acgaactata
     8041 cgcaatccct aaagatgctt ctgtagtggg gctatggtac aacaaagaat tatttgataa
     8101 tgcaggtgtc gattatccag atgaaacatg gacatgggaa acactcagac aagctgcaat
     8161 tgatttaaca gatgaatcaa aaggcatcta tggttttgca tcacatattg gaacagagac
     8221 tggctactgg ccatttattt atcaaaatgg gggagaagta tttgccgatg acaatacaaa
     8281 atcaggtttg aataccccag aagcacaaga agctttagct ttttatcgag atattgcact
     8341 tgttgatcat gcttcaccaa caattacaga attacaagat cctgataagt taagtcgttt
     8401 tcaatcaggt agattagcaa tgatgattga aggaaactgg tttgcaccgg gatttttgaa
     8461 aaatgactat ctagttgaaa atgttgcttc tgccgtgtta cctaagggaa aagttaatgc
     8521 aacaattaca aatggacttg gttgggctgc cagtgcgagt acaaaatacc cagaagaagt
     8581 gaaaaaacta atggcatttt tagctagtga ggacgccaat aaaatccaag cggaaacggg
     8641 ggcatcaatc ccagcactaa aaggctttga agacgagtgg gctgcttctt cagatacaat
     8701 tgatttgagt gcttttggaa aaatgttgga gtatggagtc cctcgtccat acaataaaga
     8761 tggattacga gctgaacaag cagaagttca aattattaac cgtattctgt ctggtgaagt
     8821 aactgttgaa gatggaaccg ctcaaattga aaaagaagtc aatgcgatat tgagcggaaa
     8881 ttaaattagt taattataca atatacaggc gaaaggattc tatttcgtct gtatattaat
     8941 ggatgaatac caaaaagggg gatattatga aaaatcgaat ttttggaaaa tatcgtaaag
     9001 atcaagtgat atggggctat atttttattt ttccaacggc attaggatta attttattaa
     9061 atatatggcc agcaattcaa acagcaattt taagttttca aaaaacggta gggttcggtg
     9121 atactgaatg ggtcggatta agaaattacc aaaaaatgtt tgcagacggt gaagtttttc
     9181 agtctttatt aaacacatta atttatgcag tggtttcgat acctagtatt gttgtattat
     9241 ccttgttggt ggcagtttta atgaatagaa aagttaaagg attgtcgatt tatcggacaa
     9301 tttattttct accagtcgtt gctgcaccat ccgcagtcgc aatgatttgg cgctggatgt
     9361 tcaataatga ttatggactg attaataaca tgttggctaa aataggtctc gatggaccag
     9421 catggttaac ggatccatcg attgcgattt attctattat cattgtagga gtttggagtg
     9481 ctattggtta taacatggtg ttattactag ccggcttata agaaattcct aaagattatt
     9541 atgaagcagc ggaaattgat ggtgctagtc caatccagca gtttttcaac attacgttac
     9601 cattagttac accaacatta ttttttgtag ttgtaacaac agttatcaat gctttccaag
     9661 tatttgatgt tatttttatg atgattactc ctaatagtac cgccatgttt aaatcacaat
     9721 cattagccta tttattctat aaacattcat ttattttaaa tgacaaggga tatggttcgg
     9781 caatcgttat gttcctttta gtgattatat taattattac tgcaattcaa atgaagttgc
     9841 agaaaaagtg ggtaaactat taaggaggct atcaaattat gaaaaataaa tttttttcaa
     9901 atgcgccaat ccatatcatg cttattttag gtggtattgc aatggcatta ccttttattt
     9961 ggatgatttt aacttcaggg aaaacgttgg ttgaatctac ccaaattcca cctacaattt
    10021 tccctgagaa atttcagcta agtaactatc aacaagtact tgatttatta ccattcatgg
    10081 ctttttatat caatacattt ttaatggttg ttggacgagt gattggctcg gttttatttt
    10141 cagcaatggc tgcatatgct tgtgcgcgtc taagttttcc agggaaaaat atccttttca
    10201 gcttagtact tattcaaatg atgattcccg gttccatttt tattactcca cagtatttat
    10261 tagcacaaaa attgggtatt ttaaatacaa ttccagcatt aatattacca ggggtagtta
    10321 gtgcgtttgg tacgttttta ttaagacaat ttttcttagg tttacctgat gaattggagg
    10381 aagcggccta tttagaaggt gccaatattt ggacgatctt tttcaaaatt atgttgccgc
    10441 ttgctaaatc aggattggtt gcacttggaa tattcacagc gttgtttgca tttaaagatt
    10501 taatgtggcc attgattgtc aatacttcaa ttgataagat gcctttagct ccgggattag
    10561 caaacttaca aggacaatat gccacgaact tcccacaatt aatggcaggt tcgttattag
    10621 caatttggcc aatagttgtg atattcatcg ttttccaaag aaaattcatt gaaggaattg
    10681 ctacaacagg tagtaaatta taaaactatt aattagtgat tggagagtaa gtacatgaca
    10741 aaaattacat ttattggtgc aggaagtact atttttgcaa aaaacgtatt aggcgatgcg
    10801 atgttaactc caagtctaca agatgcacat attgcattgt acgatattga tgaaaaacga
    10861 ctaaaagaat caaatttaat gcttaaaaat attaataaaa attcgaatga aaatcgtgct
    10921 gatattgttt cttattatga tcgcaaagaa gcgttaagag aggctgattt tgttatcaat
    10981 gctattcaag tgggtggtta taaaccttca acagtgattg attttgatat tcctaaaaaa
    11041 tatgggttgc gacaaactat tggtgacacc atggggattg gtgggatttt tagaacccta
    11101 cggacactac cagtaatgtt agactttgct aaagaaatgg aagaagtttg tccaaatgct
    11161 tggttattga actatacaaa tccgatggcg atgttaacca tgggtgtttt aaaagcgacg
    11221 agtattaaga ctgttggttt atgtcatagc gttcaagtat gcgtaccaga attgtttgag
    11281 catttaggaa tagccgatca atatgactta aatgactttc aatggaaaat tgcaggcatt
    11341 aatcatatgg cattcttgtt ggaaattaca aaaaatgggg aagatttcta tccaacaatt
    11401 cgtcagttag ctgcagaaaa accaaatcca catccagatt ctgtacgttt tgaattgttg
    11461 aaacgttttg gttactatat tacagaatca agtgaacaca atgcagaata ccatccatac
    11521 tttattaaat caaaataccc agaattgatt gacgaattag ggattccgtt ggatgaatac
    11581 ttacgtcgtt gtgaacaaca aattaatgat tgggaaagca tgcgagagga cattgtaaat
    11641 aatggggagt taacacatac gagaagccgt gaatatgctt catatatcat ggattctatt
    11701 acgacaggaa ctccgtcagt gattgcagga aatgtcttaa ataaaggctt aatcacaaac
    11761 ttaccgacag atgtttgtgt ggaagtacct tgtttagtcg ataaaaatgg cgtgcaacca
    11821 acctatgtcg gtgaattgcc aacacaatta gctgctttaa atcggacgaa tatcaatgtt
    11881 caagaactga cagtagaagc cgctttaaca ctagataaat caaaaattta tcaagcagcg
    11941 tatttagatc cacatctatc atctgaattg tctttaacag atatcaaaaa tttagtggat
    12001 gatttaatcg aagcacatgg ggactattta cctgaatata attaaaaaga agctgaaaga
    12061 gtaaggaatt ttcaattcct tactcttttc ttagttaaat ttagtaaaaa tattgacaaa
    12121 acacttagta aacagttaca ttagtgttaa caaaaagaaa acgcattcaa attgtataag
    12181 gaggagcata aaatgaagtg gtctaaaaaa attattttag cttgtatagc actaggagca
    12241 acgatctctt tgactgcttg tggaaaaaat caggaagatg caagcggtaa ggtagaaata
    12301 gagtttttta atcaaaaaaa agaaatgact caaacgattc aggagattgc taaagatttt
    12361 gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg taccgaatgc tggtgaagta
    12421 attaaaacac gtatgcttgc aggcgatgta ccagatgtca ttaatcttta tcctcaaagt
    12481 atagaattga aagaatgggc gaaagctggt tatttagagg acttaaccga agaaccgtat
    12541 ttagagaata tcaaaaatgg atatgcacaa cgttttgcta ttgaggatcg tgtatatagc
    12601 ataccattaa ccgctaatgt ctatggtttt tactataaca aaactgcttt tgaggaaatg
    12661 gggattcaag caccagaaac ttgggcagaa tttgaaaaaa tagtagctga gattaaagat
    12721 caaaacaaag taccgtttgc gattgcagga gcagaaggct ggactttaaa tggttatcac
    12781 caactagcta tagcgactgc tgctggtggt gaagaggaag cgaacaatgt ttggcgtttc
    12841 tcagaagtta atggaattaa tgctgaaagc aaagaaatgc aaaaagattt taatcgatta
    12901 gatttgttaa gagaaccaaa agctttgcaa aacaactggc aaggtgccgg ttataacgat
    12961 acagtagtta cttttacaaa aggtgatgcg ttgattatgc cgaatggttc atgggcaatg
    13021 ccgatgattc attcacaaaa tccagatttt gaagtaggaa cttttccttt tccagcagat
    13081 gaagcaggtc aaagtctaac gattggcgca ggagacctag ccttatcagt ttctgcaact
    13141 tctcaacata aagaagaagc caaacgtttt gtagaatata tgacaacacc tgaagcgatg
    13201 cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg agggcgttat cgagaatact
    13261 caggattcac cattaagtgg tttaactgaa cttgctttta ctgatcgtca tttagtttgg
    13321 ttagccaaag attggaatag tgaaaatgat ttttatacct taacaacaaa ttatttacac
    13381 aatggcaatc aaaaagccat gattaacgca ttaaatgctt tctttaatcc aatgaaagct
    13441 gacggaaggt aggaaaataa aatgaaaata aaagaaattt taaatcgtta ctgggcaatc
    13501 atttttgtag caattccaat tattttgcag atgatttttt tctactttcc aatggttcaa
    13561 ggtgcttttt acagtttaac taattggact ggtctgactt ataattttga ttttgtgggt
    13621 ttgaacaatt ataagattct tttttctgat ccgaagttta tcaaatcgat tggcttcact
    13681 ttaattgtga cgctttgcat gattgctgga caaattggtt ttggtatttt gattgcgcgg
    13741 gcgttgaacg caaatattaa agggaaaaca ttctttagag catggttctt ttttccagcg
    13801 gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa tttttaacta tggtttacca
    13861 aaaattggta gtatgttagg cattgaattt ttacaaacca gtcttttagg aacaacatgg
    13921 ggagcgattt ttgcagtaat ttttgtttta ctttggcaag gaattgccat gccagtcatc
    13981 attttcttag cgggttttca aagtattcca caagaaatta ttgaagctgc tagaatggat
    14041 ggggcgaatg ataagcaaat tttttggaat attgaaatca cctacttatt accaagtatt
    14101 tcaatggtat ttattatggc attaaaaggt ggcttaacag catttgatca aatttttgct
    14161 ttaactggtg gtggaccaag cgatgccaca acatctttgg ggttacttgt ttataattat
    14221 gcttttaaaa acaatcagtt tggttatgcg aatgctattg ctttagtctt atttatctta
    14281 attgggattg tttcactggc acaattaaaa ttatcgaaaa aatttgaaat ctaggaggtt
    14341 tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata cttgctttta ttattagggg
    14401 gcttcatgat tttagttccc ttagtttcaa cgctattcgg ttcatttaaa acaacacccg
    14461 atattatgaa taatttcttt cgttttccta atccagcgac tttggaaaat ttccgaagat
    14521 tgttagcgga tggaattggt cattactttt ggaattcggc aatcatcaca atcattgcag
    14581 tagttgctgt gacagcaatt attcctatgg ctgcttattc actagcaaga aaaatgactc
    14641 gtgggaaagc atacggattg atgtatttat ttttaatttt aggaattttt gttcctttcc
    14701 aagtaattat gattccgatt accgttatga tgagtaaaat tggtttagca aatatgtggg
    14761 gattaatctt actctatctc acttatgccg taccacagac actattttta tatgttggtt
    14821 ttattaaact aaatgtgcct atcagtctgg atgaggctgc tgaaattgat ggggctagtc
    14881 actttacaac ttatttcaaa attgttttcc caatgttaaa accaatgcat gcgacgacta
    14941 ttattcttaa tgctctatgg ttttggaatg atttcatgct accattatta attttaaatc
    15001 gtgattctaa tatgtggaca ctaccattgt ttcaatataa ttatacggga caatatttta
    15061 acgactatgg tccaagtttt gcttcctata cagtaggaat tgtcacgatt acactagttt
    15121 atcttgcttt ccaaagaagt attatagctg gaatgagcaa tggagcagtg aaataaccaa
    15181 taaattattc tcattataaa ggagttcata aacttatgat tatatttgac aatcacacac
    15241 aaacatttca tttacaaaat aaaaaaatta gctatctatt aggaatcgaa gaaaataatt
    15301 atttaacgca tctttatttt ggaaaaaaga ttacgcacta ttctggtggg tatcgctacc
    15361 cccgaacaga tcgttccttc tcacccaaca atgcaaatgc gcaagatcgt ttattatctt
    15421 tagacacatt accattagaa tttccaggat atggacatgg tgattttaga gaaccggcgc
    15481 ataatattaa attaggaaac ggtagtcgtg taaatgattt tcgttatgat agctacgaaa
    15541 ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt atatacggaa gatgatgatg
    15601 aagctgaaac gctgattatt caattgaaag acgcgattag tggattgact ttacagttgt
    15661 tctacacaat ttatcaaaat cacagtgtaa ttattcgttc tacgcaatta gtaaataata
    15721 gtgatgaaac agtggagatt aaccgcttag ctagtcaatc aattgatttt ccaaatcgtc
    15781 cactcgagtt aattcattta aatggtacct gggcaagaga acgtcaaatg actagagaaa
    15841 aaattcatac agggataaaa gtattagata gtaaacgcgg atcaagcagc catcaacaaa
    15901 atccgtttgt tgctttagta gatgccaaaa cagatgaatt tcaaggagag gcatatggat
    15961 ttaatttagt ttatagcggt aatcacgaaa cggtgattca aaaagatcca tttgaccaaa
    16021 cacgggtcat tatgggaatt aattcattta attttggttg gcaactaaaa actggcgact
    16081 catttcaaac gccagaagtc gtgatggttt atagtaatga aggtctgaat acgttatcac
    16141 agacgtatca tgaattgtat aatcaccgtt taattcgtgg aaactatcgt ttaaaagaac
    16201 gtcccacgtt aatcaacaac tgggaagcaa cgtattttga ttttaacgaa gaaaaaatca
    16261 tgaatattgt cgatgatgcg aagaacttgg gcattgaaat gtttgtttta gatgatggtt
    16321 ggtttggcgg tcgtgaaaat gacttcactt cattaggtga ttgggttgaa acagaaggca
    16381 aacttgcttg tggtttggaa aatttagcca agcaagtgca tgataacggg atgaaatttg
    16441 ggatttggtt tgaaccggaa atgatttctg aagatagtga actatttcga gcacatccag
    16501 attgggcatt gggtattcca ggtcgtgacc gctcaattag tcgaaatcaa tatgttcttg
    16561 atttttcaag acaagatgtt cgtgataata tctatcaaca aatgacggcg atttttgatc
    16621 gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa tatgacagaa gtctattctg
    16681 tcttgttgga ctcaacgatg caaggtgaag tggctcatcg ttatatgtta gggttatatg
    16741 aatttttaga aaagttaaca acgaattatc caactatttt atttgaaagt tgttcgggtg
    16801 gaggcggtcg ttttgatgca ggtttcttgt attacatgcc acaaacttgg acgagcgaca
    16861 ataccgatgc cgtggctcgt ctgaaaattc aatatggaac cagcttagtg tatccaattt
    16921 caagtatggg tgcgcatgtt tcagcagtcc caaatcatca aacaggaaga gaaacgagtt
    16981 taactattcg tggcaatgct gcgatggctg gtgtttttgg ttatgagcta gatctaacgc
    17041 aattaacttc tgaagaaaaa gcagaaatta aagaacaagt tgccttttat aaaaagcatc
    17101 gccaactttt gcaatatgga actttttatc gcttggaaag tccgtttgaa ggcaatgata
    17161 ctgcatggat gtttgtctca aaagaccaaa cagaagccat ggtcttttat ttccgagtat
    17221 tagcagaagc tgcgcatcca ttagtcacat tgaaattagc aggattagca gaagaagcaa
    17281 cttatcaaat ggaagatgta acatttgctg gcgatgaatt gatgaatctc ggtttttata
    17341 ttgactcaga actacatggt gattatgcga cacaacgctt ttatttgaaa cagatatagt
    17401 agcttgaatt tacaatataa gtgcaacacc aaaagagact cattccaaac gaagaaaata
    17461 tgttttattt acacgcattt actaaaaagc agcctgattt gaattgggaa aatgaagaag
    17521 tacgtgaaga aatctatgca atgattaact attggttaga taaaggacta ggcggattta
    17581 gaatcgatgc gatattaaac atcaaaaaaa aactagagta tggcatcttt gaatctgatg
    17641 gagaagatgg gttagccttc attgggccct ggattcttaa tcagtcgggc attgaagtgt
    17701 ggttaaaaga aatgagagag cggacattta aaccacataa tagtatgacg gtagcagaag
    17761 ccgatgttcc taatgaacgt ttagatgaat atattggtga agatggctat tattcgatgg
    17821 tttttgattt tagttatacg gatattgatg tacctgaaac tggtgaatgg tttaaagata
    17881 gccaatggac atggacggat atgcgtacca atatttttac gaatcaatta gttacgcaag
    17941 ataaaggttg gggcgcctta tatttagaaa accacgatca accacgttca attaataaat
    18001 atattccaga agaatggatc aatgattata gtaaaaaaat gttggcgacg ctttttatgt
    18061 tactaagagg tacccctttt atttatcaag gacaagaact aggaatgacc aacatagaaa
    18121 tggcatcatt agaagatttt gatgatgtag ctacgcattc tcagtataaa cgggcattag
    18181 aatatggatt ggcaccagag caagcattga aagctgtcaa taaacgaagt cgggataatt
    18241 ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag attctcaact tctgataatg
    18301 tatggttaaa agtaaatcct aattatccag aactcaatgc agaagctcag ttaaccaatc
    18361 aacagtcggt attcaattat tatcatcaat taattaaatt aagaaaaaat agtgtataca
    18421 aagacgtgct aatctacgga caatttctac cagtgaaaga taaaaatgaa catattttgc
    18481 tttatgaacg ccaattagaa gataaaatcg tattagttat cttaaacgtt acagcaacag
    18541 aacaagaata ttatgtagat gaacagtacc aacaagtatt aatcaataat tatgaagatg
    18601 ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt tgaaagtatt gtattagcta
    18661 attatggagg acaattatga aaattaaaaa cgaagcaatg ctcatcacct atgcagatag
    18721 tttaggaaaa aacatgggcg aattaaaaca agtcttagac aaacatttac aaggtgttat
    18781 tggtggtgta catctgttgc ctttttttcc atcgacaggt gatcgaggct ttgcgccgag
    18841 tgattatacg acagttgatc cagctcttgg aacatgggaa gatgtggaag cattaggtga
    18901 agattattat ttgatgtttg attttatgat taatcacatt tctcgtgaat caaaattttt
    18961 ccaagatttc aaaaaaaacc atgagcagtc accatacaaa gaaatgttta tccgtattca
    19021 tgaatttttc ccagaaaatc gaccaacaca agaagatatt gatttaatct acaagcgtaa
    19081 agacaaagcg ccgtttcaaa cggtagaatt tgcggatggt acgacagaag aggtttggaa
    19141 tacctttggg gaagaacaaa ttgatttaga tgtgaccaaa gaagttgtga aagaatttat
    19201 tcgagaaacc atcaaagata tggcgaacca tggctgttct ttaattcgat tagatgcgtt
    19261 tgcttatgcg attaaaaaat tagataccaa tgatttcttc gtggaaccag atatttggga
    19321 acttttagac gaagtgcgtc aagaagcagc gaaatatgac gtcgaattat taccagaaat
    19381 ccatgagcat tattccattc aaatgaaaat tgccaatcat gattattata tctatgactt
    19441 cgccttgcca atggtcactc tgtattcact ttatagtgga aaatcagaac gtttagccaa
    19501 ttggttgaaa atgagtccga tgaagcagtt tacaacattg gatacgcatg atggaattgg
    19561 tgttgtggac gctcgtgatt tattaacaga tgaagaattg gattacacct ctgaggaatt
    19621 gtataaagtc ggggcgaatg tgaagaaggt ctattcaagt gccaattaca ataatttaga
    19681 tatttatcaa attaacagca cctattatag tgcattaggc gataatgacc gtagctatct
    19741 gttagcacgt gcgattcaag tatttgcgcc agggattcca caaatttatt acgttgggtt
    19801 attagcagga aaaaatgata ttgaactgtt ggaagcaacg aaagaagggc gtaatattaa
    19861 tcgtcattac tatgatttag ccgaaattga agaagaagtg caacggccag tgattcaaca
    19921 attatttgca cttttaaaat ttagaaatga atctgcggcg tttgatttgg atggaacgat
    19981 tgaagtagca acgccatcag aatcagaaat agtgattaca cgtaaaaata aaaacggtag
    20041 tgatagtgca acattaacgg ctaacttgga tacgaaagaa tttatcattg agtctaaaga
    20101 tataaaaatt ttcactagtg ttgcattaaa attttaacct attccataga acaaaaaatc
    20161 ctttatacta gcacttggat gacttatcca agaccaataa aaaggactca tacatggata
    20221 agtataaaac aaatacagca tttaataaat ggttttctgc cataaaatta ggaaaacttt
    20281 ctgtcaactc ccaaaaatgt atttttgatt acaattcgta tcgtaaaaaa ctgtcttttg
    20341 aagctgtgct caagctcttt ctctacgcag ttaatgaaga aaaagaaagt ttgcgtgatt
    20401 tgagtacagc tcttatcgac cgatcgctcc aactagaaac cgatgtgtca tcgattagtc
    20461 atacccaatt gtctcgagca tttaatgcac ttgaacctaa agtgttagaa gaaatttttc
    20521 aacaactgtt agaaaaagtt caacatcaaa tcaaaccaac caagcgaaat catctgtatc
    20581 tgattgattc cagtacgttt tcgcttagtt taaaacgtca taagtgggcg actttccgtc
    20641 aaacaaaatc aggtgtcaag ttacacttaa actattgtta tatggacgat actacgatgt
    20701 atccaactga ttttacctta acaaatgccc atgaacatga tctcaatcaa ctaccagtat
    20761 tggtgaatca accagaggca acttacgtgt ttgatcgtgg gtattaggac tttgaaaaga
    20821 tggatcagat gcattgggat ggctatttct tcgtcactcg gattaaaaaa aacaccaaag
    20881 ttcacgtgat agatactctg gaaacatcgc cagaaacaga aattctacga gacgagctgg
    20941 ttcgtttagg atctaaaacc tatctaaccg ccaatttccg actagtcact gttcaagata
    21001 agaacggacg tgtgtttcaa tttatcacca atcgaatgga cgtctcttca aaagaaatct
    21061 ctgatatgta tcacgctcgt tggcaaatcg aattgttttt caagcacatc aaacaacaca
    21121 tgaccattaa aaccttcttt tctcaaagtg aaaaaggcgt gcagaatcaa ttgattttaa
    21181 cgatgatttc cgctctcctc acgtttctca tcaaattgga gacgaaaacg gaaaaatcag
    21241 tgttccaaat caaacgattc tttcgttatt tactgtttca gccctttgaa tgttgtctag
    21301 aaaaattaat tcccacatga gaaaaaagaa atttggctcg ttacttttgg ttttcagttg
    21361 aataacagat aaaaaatttt ctggaaaaag tcatcctctg cgtcagcaca gttttttttg
    21421 acttattttc agaggaaata gttttcagaa tattctaaaa tgttttatgc aacactagtg
    21481 atatttttta gaattagatt tctaatgtgc attttttgag tattaaggag caagtataca
    21541 aagatttata aagctgatta aatttgaata aaaatatgca ggaacgctac tatttttagt
    21601 agtgttcttt tttatatttg aataaaaaat gtggtggtta aatggtggtt ataacaaaaa
    21661 atgaattaat gaaattaggt tatgggaagt atcagtctga gagaatcata cgacaggcaa
    21721 aagcaattat ggtttcaaaa agctatacct attacgaaaa tagaagatta ggactagtac
    21781 ctgttgaatc tgtagaagaa gttttaggta tgaagataga ggtagaggag gattttcaga
    21841 atcatggtta attttaaaaa agataaaaat gggaaatatc attttactgc tagtttggga
    21901 ttcgacaaac gaacagggaa aagagtacaa aaaaacgtag aggggttaaa acagttaaag
    21961 aagaaaaaaa gcgtataccg aacttgtttc taattatgga aaagaagcag aaacttctca
    22021 aagtactatg ttgtttgagg agttttttta taaattattc ctaccttatt atcaagggaa
    22081 agtagaagaa agaacatata aaaatcggtt atctacaatc aaaaaaactt ttcttatttc
    22141 tttaaaatgg agttaagaga aatacgtcca attgacattc aaaagtggca aaataattta
    22201 ttgagcaaat ataaaaattc ttatgtaaga aacatgtatg ggatttttca gatgtcatta
    22261 gatcgagcag ttgttttggg actacttgaa aaaaatcctg caaaagttat tggaaatgtg
    22321 aagaaaagga aaactacagt tgatttttgg acaaaagaag aatttacaaa agttatttca
    22381 actttttata ttgatgattt gtaccaacat tttggtttta tttgtatgtg gttacttttt
    22441 atgaccgtta tgcgtttggg agaagccact gcattaactt ggaaagatat tgattttgaa
    22501 aataaaaact tgcgtgtgaa taaaacactc tattataaga gagccaaaac ctatgagttt
    22561 gtagaacccg aaacggcttc tagtattcgt acaatcgaac tagacgacaa tacaattacc
    22621 tttttaaaag cttggaagga gcgtcaagtt tcaataggag gcatgtcgtt tgtactttct
    22681 tataatggaa cacctactca gaaatataca atccgtcata ttatgagaag acatgcaacg
    22741 atcgttggtg ttcatataat ccgcatacac ggattaaggc atagtcatgc ttctttgctt
    22801 atatcacaat aaaaagatgc acttcgaatt aaagaaaggt taaaacatgc tgatgtacaa
    22861 acgactttag gaacttatgg tcatttgtat ctaaattcat tgagagagat aacaaatgat
    22921 cccacagatt atatccaaac taatttttct aaaatcaaac cgacacctat ttacgataat
    22981 aaaaaataaa tgataaacaa aagtaggatc acaccaaaag aaacacacta gaaaaactat
    23041 aatacaggct tttctagcgt gttgtttctt atttccgcct atttatacat tatactcata
    23101 aagcatttaa tgcatcgatc gtagccgcat tgtctatatg ggtatataaa gttgttgtat
    23161 tggtacttga atgtcccaat tgttgggcag ttaataccaa tgaatttgtc tgttggtaga
    23221 gtctagtagc taatgtatga cgcagtttgt gagggctgac ttgaattcta aagccttttg
    23281 aatattttgc taccatccgt tcgattgcaa taccggatat acgcttggct tctccttggt
    23341 aaattgctag aaataaagct ttttcgtttt ctgttacttt atatcgttct ttccgaatag
    23401 ctagataatt agctaaatat tccattgcga atgctgctat attgacagca tctcttttat
    23461 ttccttttct agttactacc acattattct tatctaaaga taggtcatct aaattaatat
    23521 ttataacctc ggagagtctc aatccagatc ctaaaatcag tgcattgatc gccaggtctc
    23581 tttctacatc tcgttgataa tagatcaaag ctcgtttact caaagtattt ttatattcat
    23641 tggcaatata atctagataa ttttgtgtat ccttatctag aaataatttt ttctcaatgg
    23701 cattggcacg tgcagctaac gtagttttat ctgtgatggt tgctacttga tccataggat
    23761 tattgtctaa gtatggtttt cctagaggat tattggaact tttcgaaaga tatttaaata
    23821 gaacctttaa tgccgtgata tttctattga tagtagaagt agatagacct aagttattat
    23881 tatctttgag cttagttttt gttgtctctt tttttacatt ttcttttgtg cgacgtttca
    23941 aatgagattt gtacaagttt aggtcatagg cagttaactg tcttatcgag gtgatagaag
    24001 tatcaggaac gatagactca ctcagaagcc attcataaaa gagcttataa ttttttagat
    24061 attctaaagt ggttcgaggt gattgatctt tatcctcacg atcttgaaca aaatctttta
    24121 tataatcggg catatgaagt atgattgtat ctattttttg atatatttct gtatttttca
    24181 tgtcattcat cctttcaata agtaggggag aagagtatct ctataattgg actataccaa
    24241 ttatagaatg ttttgtcaat tggtatgctc taaaatttat taatttgttt caaaaaaagc
    24301 atataaacag gtatacgttt aataatttaa aataaatggt atacataaat agggaaggaa
    24361 atagaaggga gcaatctaaa ttttttgaat tttcttaatt aattatatag aaagaacaat
    24421 ctttctatag agttagacat gagagggact atgcttgatt taaaatcaac aagcgtatat
    24481 actgtatata caaagaaaaa ggagggagaa gactatggca attaaagaaa gaaaatttag
    24541 ggaagtgggg aactcagtag tattaactat ttcaaaggag tttcttaaag aaaatgggtt
    24601 aaatcccgga gatacggttt ttttagatga agaaaaactt cgagcggcaa ttacaaaaaa
    24661 acaagcctta tctgatgaaa ttgatttagc aatatctcaa gcattaactg aatatgatac
    24721 tacttttaga gagcttgtcg ataaatgagt attcattacc tagaagccaa agatattata
    24781 aaaatgaatg taatccaaat aaaaaaatac tctcctaatg aaccccttgg tgtaaaggat
    24841 gcgaatgctt tagaaatgtg tgtagggcaa cttcgagcct cagcttttgg tgaagaggtt
    24901 tatccttcga tttatgaaaa agcagctatc ctacttatac aattgataaa gaaacatcct
    24961 tttcacaatg caaataaacg tactgctttc ttagctactt ttgtcttttt gaaaatcaat
    25021 ggacagcttt tagctattga tcaaaaagat gctgtaaatt tagcggtata tattgctacg
    25081 tatgataagg atttcgatca actaaaatat gaagtgatca acactataaa gacacatacg
    25141 cagcccattt aaggagttac tgtatgtgtc tttatagggt tattagctgc tatagcgaaa
    25201 taattgaagt aaattacaaa tagctataat tcaaatccaa ctatatagaa aacgtaaatt
    25261 ttctatatag ttggattaag ataacgacta tttattttat catattttta ctatacccta
    25321 tacttttact tggtatattt atgaataatc gcaaatttac ccctatacta ctagtttgta
    25381 agttagatta ataaaaatac taaagttatt tgttctttta ttaaatacac ctacaactta
    25441 taaattgatg gtatttagat aacacgaaag aataaaaata acagctgata gtaaagtttg
    25501 ttgacgattg ctactcaatc gaaagtgagt tagtgcgtat gaacttgctt ctgaaaactt
    25561 atgaaatgaa agacctttta catcttgcag aaatcgttac tgggattaaa ctaagcaata
    25621 aaaactaatg ttcccctcat tctttgaact tctactattt gatttattta ttctgataaa
    25681 tgacgatttc accttcagga tcttcaaatc ccgtatagaa tttatttaag atctcttcag
    25741 tatcagtaat aggataggcg ataggccaca taccctttga tggttagaat aaactgctca
    25801 aaatctgtat cgtatgttgg ctttagcata ttaacaaagg ctatctccaa attataaacc
    25861 ataagcgttg aatcgttctt aacaatttca attcttccta acccaaagtc agaagatata
    25921 attaccggac ttgccttacc cttcatgtgg gttgttgaga ttccatattt aaagggaatt
    25981 gtcggtacta tcggcggtct gaaaaacatc ccaaaaataa aaaaatcttt cccctgctag
    26041 tctcctaagt ccctttgtag cggatatggc accataatct cattttatcg aagtgagtca
    26101 tggtttatct gttggccaac caaaattgat acaatctaac gatgcaccct ctacggcatt
    26161 ctaggggagt gcacttgcat tcacccaata aaaaccggca gttagcaaag aacttatgta
    26221 gtcgtatgtt cttactgtta acatacaaga ggtctccttt ccacaattgc ttagcagtaa
    26281 acgggaattt acaaagctaa tctcttccat tttcttatct ttgttctaaa agtcccaaat
    26341 ggagcaactg tatttacatg aataaattta tatacttccc atacagccgt tttggttgcc
    26401 tcgtctgccc attttctttt gtgtgcagtg aatagatctt catcactcat tgaatcaatc
    26461 atcatgttaa tatcatcaat attacgattg agaatttcct ctaattcttt caatggtaga
    26521 tgtgcatagg tatttgtaaa ccacttatat aactctccta actgattcca tttaaattga
    26581 tcagaaggcg ttttaacaga taatccattt ctttcatcat tttcccattt tagaattaaa
    26641 ttcgtccaac caacttgata tgctaaattt tcagcaggtg ttctatccac ttcctcaact
    26701 cttttatcct tcaattcttc aggaatagtt tcaaattcta aaatatattt agtataagtc
    26761 ctatttattt ctttttttaa ttcttcttta tttttatatg ttctcattat attacctcca
    26821 taaattccta attgtctcct ttaattcatc tataatttca tccacgatat tttaatccgt
    26881 tctacaatgc atatctgata tgcattgact gtctaactca tatgtgaatg atttataaaa
    26941 tatcccaacg tctcatctaa agaaatgttg atcgtcagca aggagctaaa aaatacgaat
    27001 atatttcttt tgtagtaaaa tcggactggt ttgcatatca ttttcttcgt aaatatcaaa
    27061 atcatgttaa ctactatcaa ttactaataa aaactaccac tttaagcgtt aagaactgtt
    27121 aagtttagtt aagtgaggat agttcacaaa atccattgtt aaacttcacg aaagtggtaa
    27181 aactcaaaat gctttatata ggattacagc atcgctcttt cttcgattgc gcgttgggta
    27241 aaaaatatta tgaagtaaaa attgacgatg acacgttcct tacggctcat cagatgaaac
    27301 agctacaaaa acgaaatgat caactcgaag aaaatttaat tctaaaaaag cgattgccat
    27361 attcacgcct cgctcgacga actattaaag gtcaagattt acctgaagat catccttttt
    27421 aggaaattga agctttttat gttgcacaat gcatttataa tactacaatg atgttttcac
    27481 cagatattct tctagggtgc ggtgtaatga aacagataca cttgcaaaag aaaattcacc
    27541 gcgaatttac taaaattatg aataattatg ttaatcttcc gaaatttttt tccaaaatta
    27601 agggatcaag ctggtgttat agattgttta aggttggcaa aaaaataact tctttttaaa
    27661 caattataga aaaccgatac tctgttaagt tggttgacac ggtgtcgatt tttatgactt
    27721 tcttattctg aaacttcact aacggacagt acctaaaaag tctatagtat ggacgtttac
    27781 gagtactatt ttatcaaaaa tgctgctcat ttcaacttat atgttttagt gaactacccc
    27841 cacttagctt ggataggcta caattaacta gacagaaaaa ttaaggtgtg tagactagaa
    27901 gaaaacatac caggaggaat ttttatgtct aagagaacac gaagaacttt ttcacaagaa
    27961 ttcaagcaac aaatcgtcaa tctttactta gctggaaagc cacgtgtaga aatcattcga
    28021 gaatatgaac taacggcttc agcatttgac aaatgggtaa agcaatctaa aacgagt
//
LOCUS       DAYYBH010000029        27588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000029 DAYYBH010000000
VERSION     DAYYBH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.029"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACHAUG_001442"
     CDS             275..1753
                     /locus_tag="ACHAUG_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480536.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2030..4090
                     /locus_tag="ACHAUG_001443"
     CDS             2030..4090
                     /locus_tag="ACHAUG_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8480537.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4173..4742
                     /locus_tag="ACHAUG_001444"
     CDS             4173..4742
                     /locus_tag="ACHAUG_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8480538.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4917..5723)
                     /locus_tag="ACHAUG_001445"
     CDS             complement(4917..5723)
                     /locus_tag="ACHAUG_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480539.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5861..7375
                     /locus_tag="ACHAUG_001446"
     CDS             5861..7375
                     /locus_tag="ACHAUG_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8480540.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7778..8761)
                     /locus_tag="ACHAUG_001447"
     CDS             complement(7778..8761)
                     /locus_tag="ACHAUG_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8480541.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8766..9413)
                     /locus_tag="ACHAUG_001448"
     CDS             complement(8766..9413)
                     /locus_tag="ACHAUG_001448"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8480542.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9498..10130)
                     /locus_tag="ACHAUG_001449"
     CDS             complement(9498..10130)
                     /locus_tag="ACHAUG_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8480543.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="ACHAUG_001450"
     CDS             complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="ACHAUG_001450"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8480544.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10694..11431)
                     /locus_tag="ACHAUG_001451"
     CDS             complement(10694..11431)
                     /locus_tag="ACHAUG_001451"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8480545.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11428..12417)
                     /locus_tag="ACHAUG_001452"
     CDS             complement(11428..12417)
                     /locus_tag="ACHAUG_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8480546.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12675..13217
                     /locus_tag="ACHAUG_001453"
     CDS             12675..13217
                     /locus_tag="ACHAUG_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8480547.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13234..14319
                     /locus_tag="ACHAUG_001454"
     CDS             13234..14319
                     /locus_tag="ACHAUG_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480548.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14331..15137
                     /locus_tag="ACHAUG_001455"
     CDS             14331..15137
                     /locus_tag="ACHAUG_001455"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480549.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15138..15965
                     /locus_tag="ACHAUG_001456"
     CDS             15138..15965
                     /locus_tag="ACHAUG_001456"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480550.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15962..17035
                     /locus_tag="ACHAUG_001457"
     CDS             15962..17035
                     /locus_tag="ACHAUG_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8480551.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17463..20159
                     /locus_tag="ACHAUG_001458"
     CDS             17463..20159
                     /locus_tag="ACHAUG_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8480552.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            20224..20724
                     /locus_tag="ACHAUG_001459"
     CDS             20224..20724
                     /locus_tag="ACHAUG_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8480553.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            20736..21230
                     /locus_tag="ACHAUG_001460"
     CDS             20736..21230
                     /locus_tag="ACHAUG_001460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8480554.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21251..22060
                     /locus_tag="ACHAUG_001461"
     CDS             21251..22060
                     /locus_tag="ACHAUG_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8480555.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            22057..22878
                     /locus_tag="ACHAUG_001462"
     CDS             22057..22878
                     /locus_tag="ACHAUG_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8480556.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22929..25028
                     /locus_tag="ACHAUG_001463"
     CDS             22929..25028
                     /locus_tag="ACHAUG_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8480557.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            25025..26143
                     /locus_tag="ACHAUG_001464"
     CDS             25025..26143
                     /locus_tag="ACHAUG_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8480558.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26182..27060
                     /locus_tag="ACHAUG_001465"
     CDS             26182..27060
                     /locus_tag="ACHAUG_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8480559.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27206..27403
                     /locus_tag="ACHAUG_001466"
     CDS             27206..27403
                     /locus_tag="ACHAUG_001466"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8480560.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            27387..>27588
                     /locus_tag="ACHAUG_001467"
     CDS             27387..>27588
                     /locus_tag="ACHAUG_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480561.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVV"
BASE COUNT         8823 a         4728 c         5757 g         8280 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aactagatat aactagatat attacttaac
     1861 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
     1921 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
     1981 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
     2041 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
     2101 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
     2161 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
     2221 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
     2281 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
     2341 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
     2401 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
     2461 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
     2521 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
     2581 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
     2641 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
     2701 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
     2761 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
     2821 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     2881 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     2941 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     3001 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     3061 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     3121 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     3181 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     3241 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     3301 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     3361 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     3421 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     3481 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     3541 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     3601 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     3661 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     3721 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     3781 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     3841 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     3901 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     3961 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     4021 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     4081 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     4141 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     4201 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     4261 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     4321 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     4381 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     4441 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     4501 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     4561 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     4621 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     4681 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     4741 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     4801 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     4861 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     4921 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     4981 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     5041 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     5101 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     5161 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     5221 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     5281 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     5341 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     5401 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     5461 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     5521 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     5581 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     5641 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     5701 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     5761 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     5821 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     5881 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     5941 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     6001 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     6061 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     6121 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     6181 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     6241 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     6301 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     6361 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     6421 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     6481 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     6541 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     6601 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     6661 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     6721 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     6781 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     6841 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     6901 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     6961 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     7021 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     7081 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     7141 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     7201 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     7261 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     7321 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     7381 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     7441 ttgaacactg tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     7501 gccaagtaaa aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa
     7561 gcttttgaac accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt
     7621 ctcacgctct aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg
     7681 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     7741 cgcttctgtc ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga
     7801 tttttccgat gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt
     7861 ggaatgtacg gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt
     7921 gcataaaagt ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac
     7981 caagcagttg gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc
     8041 cacctagcat accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt
     8101 cccagtaaga agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt
     8161 ttcgtacctc ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt
     8221 ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa
     8281 gctgtctagc taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga
     8341 cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa
     8401 cttctgagat tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat
     8461 cggcaggcat cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg
     8521 tcccaggagt aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga
     8581 ctcccaaaat agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc
     8641 gattgacagc tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag
     8701 gcacttcaat ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca
     8761 tggatctatt cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca
     8821 gaaagtacta gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca
     8881 gcaactgtag agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg
     8941 gaagtaactt cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta
     9001 gttcgtttca gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca
     9061 ggccattttg ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc
     9121 gcattttcag ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc
     9181 tttccttgag agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt
     9241 ttttccacgg accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat
     9301 gtttgggaag caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct
     9361 tttatcaggt gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc
     9421 atcctttcga cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag
     9481 atcggctacg atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca
     9541 ttgcatactg ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa
     9601 ataaccgcta gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa
     9661 tcttgctgga ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg
     9721 gaaagtccag ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga
     9781 cctttataaa cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc
     9841 aaggctttcc agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa
     9901 gacaaaggtg tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca
     9961 agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg
    10021 gttgttgcat tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc
    10081 aaaatagcta aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct
    10141 tttttctaag catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata
    10201 tcagctaagg caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt
    10261 tcctgatagc cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac
    10321 atattcgtgt tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg
    10381 actgttgaaa gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta
    10441 gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc
    10501 ggatttgatt cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt
    10561 aatggcgtga taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat
    10621 ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct
    10681 agtaagatct ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa
    10741 tctcagattt actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt
    10801 gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta
    10861 tcagactatt ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga
    10921 aaccaatgat ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt
    10981 tcttcaaaac gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg
    11041 tctgttcatt ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt
    11101 tttttgccag ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac
    11161 tgtggataat cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt
    11221 ccagtgcaga ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt
    11281 gtttcgtagt cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg
    11341 cttgccctaa tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag
    11401 tcgttcctcc agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct
    11461 tccttggtgt gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat
    11521 ctcttggacg accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag
    11581 acgttgatct attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat
    11641 aaaaggcaag acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc
    11701 accgttataa gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat
    11761 gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg
    11821 tcgtacgact tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga
    11881 aaaggtaatg attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac
    11941 accgccagaa gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa
    12001 agcgttcaca cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag
    12061 cgtcatattt tttggtagag gaactaacca gtctcctggt actttttgat attggctata
    12121 accgcctgga tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga
    12181 aaaacgtgga ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg
    12241 atatgttcgt atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc
    12301 agaataggca atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact
    12361 gattgctgta tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg
    12421 tgagacactc cttttaaaat tttcttttat cataccataa agcctactct tttattcttg
    12481 ttcttccgta aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt
    12541 attccaaaag ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa
    12601 taaatttcgt atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg
    12661 attcggagga aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt
    12721 tgacacaaga agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg
    12781 aacgagattt aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg
    12841 tgactccaga agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag
    12901 aagatcatac ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg
    12961 attcgaatga aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata
    13021 agacgtttga accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt
    13081 taagcttagg agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga
    13141 caaaacaaca tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa
    13201 cgcaatcata tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat
    13261 caagtagtga aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa
    13321 caaggaaaat tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt
    13381 attatagctg gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg
    13441 aatgatttgc cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt
    13501 cctcatatga atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa
    13561 tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa
    13621 aaacgggaga ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca
    13681 attgtaaatg aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa
    13741 ctacgtacag atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt
    13801 atttttgtta ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg
    13861 aataaaggga aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat
    13921 catttcgttg ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat
    13981 aatctagttg agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat
    14041 gaaccagttg aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga
    14101 aaactagtcg taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt
    14161 tatgatgaac agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc
    14221 aaagtaggat tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa
    14281 gaagaattcg atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa
    14341 tgcgcaggat ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg
    14401 tattaatgat catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt
    14461 atctatctta ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg
    14521 catttttgat cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa
    14581 aactgaagca caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc
    14641 tgcttaaagc ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct
    14701 ttgattttat cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg
    14761 tggcaacgta tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga
    14821 tgaatccttc tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc
    14881 gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta
    14941 ttccttctct ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt
    15001 taggaacggc tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa
    15061 tcggtgtgat tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa
    15121 aaggaggtcg ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct
    15181 ttttattctt atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa
    15241 cgatgaacag ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac
    15301 gtttgatcat tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca
    15361 cgatcatcgg gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga
    15421 cagcgttgtt gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta
    15481 gtttcttgat tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt
    15541 cccatattgc tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa
    15601 tgaacgattc aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca
    15661 aaaatatcat tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta
    15721 cctattctct tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc
    15781 tctcagtaga gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa
    15841 gtgcgttagt tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag
    15901 aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc
    15961 gatgaaaaaa ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct
    16021 tggtgttcgt caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta
    16081 taactgggga gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata
    16141 caaagtgaat tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg
    16201 tgggacaaac tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga
    16261 aaaactagta cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg
    16321 atttttagac ttggattttg atccagataa tcagtattcg attccatatt tttggggaac
    16381 tttagggatc gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga
    16441 tttgtggaag ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat
    16501 ggggctttct ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagctgac
    16561 agaagcagca aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga
    16621 gatcaaaatg tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc
    16681 tgccgatatg atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa
    16741 tctttggttt gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga
    16801 tttcatcaac tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta
    16861 ttctacacca aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca
    16921 attctatcca tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa
    16981 atacctaggc atctataatg acctattctt agaattcaag atgtatagga gataatggca
    17041 aaactcagta agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct
    17101 atctgagttt tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt
    17161 tattttttct accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg
    17221 cccgagacgg gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa
    17281 actatgcaaa tatacttaac ccttctgtaa tctattaata aaactaatat atgaatccag
    17341 ttcaatattt gtagctatga aaaaacataa aaatcatatt agataaaaac taaattggta
    17401 gataaattat tttgtcagat atctgaggca aatattcgac tatttgtgtt tcaaagtaaa
    17461 aattggaata caaatataga atagacatat tgcagccgat agtttattat gcttttagac
    17521 acattaaact ggaggaaaaa atgaaaaaga gcgctaaaga tcgagttttt gatgagctga
    17581 tacgactaac agggaatatg tcttctgtca ctgccgagaa aatttcgaag aatctaggta
    17641 tctctcgtca gaatgccagc cattatttga cgagattagt ggaagataaa aaggtcgaga
    17701 aattaccggg aaagcctgtt ttgtggaagc ctttagatga atatgcggtt gtagataata
    17761 cggagcaaat aaacgaagcc tttcattctg tagttggtca tgacggaagt ctgagagagg
    17821 tcattcaaaa atgtatagcc gctgtcaaat atccgcctaa cggactcagt gtattaataa
    17881 atggagccac gggtgttgga aaaagttttt tagccacaaa aatttttgaa tacgctatcc
    17941 atgagcaaat tattgaaaaa gatgcaccat ttgctatctt aaactgtgcc gattatgcgg
    18001 acaatcccga actgctttcg gcaacactgt ttggatacaa gaagggggcc tttacggggg
    18061 ctgaaaagga taccgatggt ttattggcaa cggccaacaa cggctatctt tttctcgatg
    18121 aggtccatcg cctatcgaaa gaaaatcaag aaaagctctt tctatttatt gatactggaa
    18181 actatcgtcc agtcggtgaa aatgtaaatt ggcattctgc taaggttcgt tttattttcg
    18241 ccacgacgga aaaaggtgaa aattatttat tagacacctt tgatcggcgt attcaaatta
    18301 gtgttatgtt gccaactttt gaagatcgcc ccattcgaga acgattagag ctgattcaat
    18361 tattttttca aaatgaagcg gatgttttac aaaaagatat tattgtttca agagaagcct
    18421 tgtctttaat gttatctcat ccctttagtg ggaatattgg taagctgaag aatatcatta
    18481 aaatcagttg tgcggatgtg tatagtcgaa cacaagaaga accattaacg attgggaaaa
    18541 atgagatctt gattcagtta aatatgctgg aatccgaagt agaaagcttg ccgattgcta
    18601 gtttattgat aaagaaaaat caaaagataa aaacggatcg aatcaaaccg tttgattatg
    18661 attcgatttt ggaaaaaatt actcagctga taaatgaagg taatttcaat gaaaattttt
    18721 cttcgataaa gaatgaagta cagcatttgg tgcatcacat tgataaggat tcttttttat
    18781 caatgagtag aaaattgatg attcagatgt ttagaaaggt ctgggtatcc gttttaggaa
    18841 aaacgtacgg attgacgacg agtggacctg ttgcagaaat tattagcaga tcctatgtca
    18901 tttccccaag ctataagaca gacttaaccg ggataaatcg tacattagct gcaaatctac
    18961 ctcggacgac ttacttatgt aatcagttct tggagcattt accaccgctc tcagaaaagg
    19021 acgagaactt tctaagactg cttattgtcg tgactttatc tgattatgta gatgaatcca
    19081 tcgagctgaa gggcctgttg cttgctcacg gagatagtac ggctagcagc attcaagcag
    19141 tagtgaatca attatgcggc aattatgttt ttgaagcgtt ggatatgcca gtagagagta
    19201 atgtgtcaga gattgtcgaa tcgacaagga atttcgtgaa acggcaagtg aatacagata
    19261 gtctagtgct gatcgtagat atgggctctt taaatcaaat ttacacacag atcaagaacg
    19321 atttaaaagg ggagttactt cttctgaata atttgacgac ttccattgca cttgatattg
    19381 gcttgaagat gacgatgaat gcgccattta aacagattgc ggaggatgca gcagacaagt
    19441 attcaatcaa tgttcaatac tttgaaggct tttctcaaag tagcaacatt atcatttcct
    19501 gtatgtcggg tttggggatt tcggacaaat tgaaagatgt ttttcgtcat catttatcgg
    19561 atgaatccat cgaagttttc acgaaggact acagagaact acgtgaatta atcgaccaaa
    19621 atgatgaaac ttattttgac aagacaaaat tggtcatcac gacttcggat cttccacgtt
    19681 ccttttcaat tcctaatatt aatgtttatg acatcttaga cggtgatgga gcagccaatt
    19741 tggagcatat tttgagtcct gaaatgaagc cggcagaatt taaccgcttg attcaggagc
    19801 tagtaaaatt cttttcgttg gaaggaattg cggaccgttt gaactttctg aacccaacga
    19861 tcgtgatgag tgaagtggaa acagtcattt ctaaatatga aaattactat catttcactt
    19921 taaatggccg tgtaaaacta aatctctata tgcatacggc attgatgatg gaacgtttat
    19981 tcttggctcg gacagaaaaa tctgaagaac cgaattatga tttgaaacca gatgaagatg
    20041 aattttatta tgtttctaga agtatttttc aatcaatgga gatgaaatat aattttaaag
    20101 tcaatgctta tgagctttcg ctgctctatg aattattagg accgtatatt caaaaataaa
    20161 gtgtctaaaa aagtgtctaa tctgtttttt ttaggttaga cacttttttt aatgtgaatt
    20221 aacgtgatta aaccaatgtt tttaaaagtt ggcacaggaa ttgcttatat aaaagtgaac
    20281 agaaagaaag gaattaacca tatgaagaaa attctaatag cgactcacgg tcacttagct
    20341 tcaggctttc ttagcagtat tcaattactg acggggaagg ttcaagagat tacggtgatt
    20401 aatgcttatg tggatgagtg tgattttgaa caggagttga atgtatttgt aaaccaggtg
    20461 aacgagcagg atcagatttt tgtttttacc gatttatttg gcggaagtgt caatcagaag
    20521 attaccaaaa cctttttgga gaaagagatt gctgtaacta ttttgtctgg ctttaatctg
    20581 ccaattctgc tcgaaatagt attggcaacc aaggaattaa caaaggagga tattcaggaa
    20641 ctggtaaata aatgccaaca ggaattaaag atgaccattc ttgatgaggt acatgtagta
    20701 aatgaagagg aagatttttt ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg
    20761 aacgattaat tcatggacaa gtagcgatga cttggacaaa gagtctaaat ttaaccgggt
    20821 tagtagtagc aagcgatgag gcggctacca acgatattca aaagatgaca ttgaagatgg
    20881 ctgcaccatc gaatgtcaaa gtcattatta aaacagttga tgacgcgatt catttgttaa
    20941 aagatccgcg ggcgaaggac atgcgcttga tggttttagt ccctaatgtc aaagatgcgg
    21001 taaaagttgc gaaagctttc ccaacggaaa tcgaattaat gaacgtgggg aatgctggga
    21061 aaatgacacc gccatcggaa gataagaaaa ccttatcaaa ggaagtgatg ttaacgacgt
    21121 cagaaattca agacttaaaa gaattagttg aactttatcc agataccgtt tttcagccta
    21181 caccggcgat ggaaaaacgt tcagctatct ctattttgag tgatttttag aaaaaggaag
    21241 gagaaaaatt atgtatgatg catttgtagt cgctttggcc gtttttattg gagttgctgg
    21301 acatgaaatg ttcgggatgt ccatgctgag tcgtccgatt gttgttgctc cgttagcagg
    21361 agcgttgatg ggggatttgc agacggggtt gattgtcggg gcgtcactag aagccatctt
    21421 tatgggtgtc gtcaatatcg gaatctctag tacggcggag ccttctttgg ctgctgggct
    21481 ggctacagcg ttcgcaatca aaatgggagg aaacgtggat gcgattattc ccattgtctt
    21541 tccactagca gtattaggat tacagttaat gaatatgatt ttttcttttg tatgtggacc
    21601 gatggctgct aaatttttca aatttgcaaa atctggtgat caaaaaggaa tcatcaatct
    21661 gcacttcttt atgtggtttg tccactatgc gctttatgct cttattccat tctttgccgt
    21721 tttatttgga gcagatgttg tcaaaagcgt cttggataat attccagagg tcatcatgaa
    21781 cggattaaca gtcgctggaa atctgctgcc ggcagttggg atggcgatgc tgttacggat
    21841 gctttggagt aaaaatattg ctgtgtggtt tttctttggg gccgttgtta tggcgtatct
    21901 cgagttacca ttgattgcaa ttgccgcagt tggaacgatt attgcaattg ttatagccca
    21961 aagagatcta caactaaaaa atatatctgt tcatgcagtt caaagcgatg ctgctgtaaa
    22021 tgcaacgctt gaccaagaag aggaggattt ctttcaatga aattagctga tggaatttct
    22081 aaagatgaga aaaaattggt tcggaagatg ttttggcgct cgatgactat gtatgcttcg
    22141 gtgaatccgg tgacgatggg cggcggtggt ttcgcctatt ctatgatgcc cttcattaat
    22201 catttctaca aaagtgaaga agatcgagct gcggcgctgg aacgtcatac acagtatttt
    22261 tcgacgacca ttccttgcgc gtcctttgtc atggggattg cggcatcgat ggagaaggaa
    22321 aattctgaaa aagctggttt tgacgtggaa agtatcaacg cgatcaaaac gagtttgatg
    22381 ggaccattgg ctggaattgg cgattcattg ttctggggag tttggcgtgt cgttaccgcg
    22441 ggactagcga tcaacttagc caatgccgga aatattttgg caccaattat ctttttagtt
    22501 atgttcaata tacctaatta cttgatgcga tactatggcg gcgttttagg ctattcatta
    22561 ggctctaaat atattgaaaa gctgtatgcg aacgggttaa ttgaaattct gacaaaagct
    22621 gctagtatct tgggtctgat catggtaggt gcaatgacga tcagtatggt caaattcaat
    22681 accgttttga gcttttcaat gaagggcgaa gaactaatga atgtccaaga aatcctcgat
    22741 agtattttta aaggaattgt accgttattg gtaacattga gctgtttcaa attactagat
    22801 aaaaaagtcg gcgtcatcaa aattattttt ggaatggtcg tcttgggaat tttattgtca
    22861 ttacttggaa tcgcatagta ggaagctgca gctactaggc tgcagttttt ttagcaagga
    22921 gggaaaagat gactattaat gaagtaattt attcacccaa acacccattt tttaatgctg
    22981 agttaaacga cggaaatgtg actacttcaa acgaatacag ctactatcgg aatattcctg
    23041 atgtaaaggg attgaccttc gatgtaagta atcaaaagac attagcaaac cttgatgttt
    23101 gcgggacgat tcaaacgttg accttttatc gagaaaattt actaactgag gaaaaaccag
    23161 gtgtttgggt caataaacag ctctctcaaa ccaattcgtt atccttaaag gttgaagtaa
    23221 acgggattgt ctaccatttg tcagaatcgg atcatcgtat tgaagtcgat gtgattcaag
    23281 actgtttgcc tagatatatt catcattatt cgacgtttaa ggtcattgta gtatcctttg
    23341 ctcccatact ggctaataag cgtttaagta tgcttgttca acaggtttac attgtgaatg
    23401 aaacacacga aagccttcaa gtttcagtga aagatgtgcc ttcgtatcaa gagaagtatt
    23461 cggatcagca aaatgtgttg atttctcaaa aaggaaataa ggacacgatc gcacaacagg
    23521 aagtggcaag cttcgccatt gcttttgtgg acccaaatgc ttttgaagaa gtaatgactt
    23581 ttcaggaatc tgatatcgaa aaatggctga aggaaacatt gaactatttt gctcagctat
    23641 ttgggcagct gtcaatgcca aacgatagag ttgtccatct ctatcgtcgt gcactttatc
    23701 aaagcttttc tagtttcggt atgaatcgac agggacaggt cgtcggcagc aattggggca
    23761 gctatcctgc tactaatcga atttggaata aggatatgta ttacgcatca ttgccattca
    23821 ccatgtttga accggagtta tgtcaaaaaa cggttctatg gtttgatcaa tatggcgtta
    23881 agtttcctgg ttcaaaattt acaggaggta tcaatcattc tctgagtaat tcgttatcca
    23941 gtgcgttact ggctagtctt tattttgaac atactgggga tacaaaattc tttgagcggt
    24001 gtccgcaggt tctactgaat gcttcaagaa ttattgagga catattagct caacgtcggt
    24061 caggggaacc aatgcttttt ccatctgttt ggttatcaga cgcctttgca cttggaaagt
    24121 atcataccgg cagcaatctt tgtctttgga aggcctgtat tggacttgca gaaatttttg
    24181 aagccctaga acaactgagt cttagcaagc gatacaaaaa cattgcctgt aaactaaagg
    24241 aatcaatttg taaacaaatg actatcgatg gtccttttgg tgagcaattt ttggagggaa
    24301 tcggtgatga ggagaaaaca acctattcag tgaagcacta tcaaaaaccg attcttgagc
    24361 aagggttaat ctttttaagt gatgtgattg tggatggaaa aattaattta ttgatgcatg
    24421 atggagaaga atcagacact acgttgatgc ctttttatca atttttagat aataaagatc
    24481 atttatatca gaacaccatg gccttttctg ctagttcttt caatccaacc tatagcgagg
    24541 aaatcaaagg catcacttgg ggattagaat ccggcgccac ttttcctggc tttatcactg
    24601 ttttaatgag cgagctgcac aaccctcaag ctttcgtaac ccgattagaa gaactaattt
    24661 gtttggcaga tttagacggt tcgtggtggt ggtggccgta tcgcttagag gctcaacaag
    24721 gagaagtcgt gcgagacttt ggctgcggca aatgcggctg ggcatcgggt ctttttgtca
    24781 gcctaatgat cagtcaatat ttcggattga aattcaaaaa aggagtattg caaattgatc
    24841 ctgttgataa tttaacgttt tcttggaaga atttgaagtt cggtcaagcc ttcatttcga
    24901 ttgaatgtac gcaatccgag ttgagtattt caaatttgtc agaaaagcca ttgaaaataa
    24961 gtgatacgaa aaaaattctt cacgtagaaa agggaaaaac gattcacatt caaaggagaa
    25021 aaagatgagt atactaaaaa atattaccca atttaaaaaa atccagagtc gagcaatcac
    25081 tcccgaaaat ccaacaggcg agaaaggtaa agctgcgatg ttgccaagcg agctaggacc
    25141 aagccgcaaa ggtcgtggcg caattccgct gacttctggt gaagaaactg tgattgcgga
    25201 tattactgga acgggaataa ttcgtcatat gtggatgacg attcgtgatc gaacagaatt
    25261 tggcagtttt gttttacgtg atttaatctt aagaatttat tgggatgggt cggatagccc
    25321 tgctgttgaa agtcctttag gggatttctt ttgtaatggg tttggagagc gttgcgaggt
    25381 gaactcgttg ccgatagtgg ttaatcctac agggggcttt aattcctatt tcgaaatgcc
    25441 tttccaaaca tccgctaaaa ttacgatcga gaatttacat ccgaaggata tgtcctcatt
    25501 tttttatacg ataaattacg ctttggtgga cgaattggaa gaaaatagcc tatattttca
    25561 cgcaaattgg catcgggaac gcattaccaa attacaggag gattataagt taattgatcg
    25621 attaaagggt caaggctatt acgtgggtac ttaccttgcg cttacctgcc tagaacgtta
    25681 ttggtggggc gaaggcgagt ttaaattcta cattgatggg gatagtgagt atccgaccgt
    25741 aacctcaaca ggttcagaag attattttgg cggggcgtgg gcctttcatg agtacgatga
    25801 gttcggacgt ccacatgcga aaaactattc aacagccttt ttagggtatc cttaccaatc
    25861 aaatagagat gccacaagag atcgttttga aacgggaaaa ttgaatgccg ttcatgcctt
    25921 tggtgacgac gcaatgccac gacatggtct ttatcgttgg catcttcaag accccatctg
    25981 ttttaccgaa gatttatcgg tcactttaca acaaatcggc aacgatgatg ttcgcttatt
    26041 tgagcggcaa gacgatgttg ctagtgtcgc ttactggtat tcaacagaag cttcaggaaa
    26101 tactaaacgt catttcactg atcgagaacg cttgccgcgt taatgaagtg tttttcacat
    26161 attcgaaggg aggaacctat gatgatatat ggggcaattg aagctggagg gactaaattt
    26221 gtctgtgcag tcggtactga ggaattaacg attttggaaa gagtcagttt tcccacaaca
    26281 actccagttg aaacaatgaa gcaggtcatt gattttttta aaccttttaa ggaacaattg
    26341 gcaggcatcg gggtcggctg ctttggtcca attgatattc aatgcaaatc aacgagtttc
    26401 ggtcatatca catcaacacc aaaacttgct tggcaaaatt tcaattttgt aggaacgcta
    26461 aagcaatatt tcaatattcc gattgaatgg acaacagatg tcaatgcggc ttgctatggt
    26521 gaatatgttg ctggttctgg caaagggtta gcaggtgttg catacttcac tgtgggaaca
    26581 ggtatcggag gtggtgcctt gttcaatggg actttcgtgg aagggttcag tcatccggaa
    26641 atggggcata tgttgatcaa gcgacatcca aaggacaatt tttcaggaaa ctgtcccttc
    26701 caccaagatt gtttagaggg gatggcagca ggaccagcga ttgaaaaacg gttgggtgta
    26761 aaaggccaga atttattggc tgacgattcg ttttggcaga ttgaagcctt ttaccttgcc
    26821 caatgtgcat acaatacaac actgatgttt tcgccagata gaattatttt tggcggtggt
    26881 gtaatgaaac aagaacacat gaagaaaaaa gttcaggata aatttgttga gcttataaat
    26941 ggatatgttg aaataccacc aattgatagc tacattatta cgcccgaatt aggtgataat
    27001 gccggaataa tcggcggatt agcattggcc agaaaagcgg taagaaataa gcaaccataa
    27061 ttaatgaatg tttcattgat actcatgtca aaagtattta accgagtaat caaatgatca
    27121 aatgaagttg gataaagcga aaatgaattc aaataaaaag gctctaagtc aaatcgtgtt
    27181 gttttcatta aatcactgaa aggaaatgct tctgcgtttc cttttttctt tttgttgtgc
    27241 gctgtatacc ttgaggatat tcactggtgg cgtacactta aatagtaata tattgtgtta
    27301 ctttatgtct gtggtaatag ttatagttgc ttaccaatgt atgttcattt ttcgaaatgt
    27361 tgacttcata atccttggct taatgtttga atttttttgg tgatagattt tttcagccgt
    27421 ataaaataaa tcgtaattct attatagaga gttgtgatta taaaaagata gcaagaaaaa
    27481 aaaagaaagt ttctatacat tttaaatatt ttattcctat ttctattgct aaaaaagtag
    27541 aaattctata tttaccttta actctcgctt ctcttgcaaa tgtagtta
//
LOCUS       DAYYBH010000030        26787 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000030 DAYYBH010000000
VERSION     DAYYBH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.030"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACHAUG_001468"
     CDS             complement(104..1336)
                     /locus_tag="ACHAUG_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8480562.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACHAUG_001469"
     CDS             1591..2160
                     /locus_tag="ACHAUG_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8480563.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACHAUG_001470"
     CDS             complement(2338..2778)
                     /locus_tag="ACHAUG_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8480564.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACHAUG_001471"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACHAUG_001471"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8480565.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACHAUG_001472"
     CDS             3732..4655
                     /locus_tag="ACHAUG_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8480566.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACHAUG_001473"
     CDS             4731..5870
                     /locus_tag="ACHAUG_001473"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8480567.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACHAUG_001474"
     CDS             5863..6663
                     /locus_tag="ACHAUG_001474"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8480568.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACHAUG_001475"
     CDS             6663..7490
                     /locus_tag="ACHAUG_001475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8480569.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACHAUG_001476"
     CDS             7495..8202
                     /locus_tag="ACHAUG_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8480570.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACHAUG_001477"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACHAUG_001477"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8480571.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACHAUG_001478"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACHAUG_001478"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8480572.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACHAUG_001479"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACHAUG_001479"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8480573.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACHAUG_001480"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACHAUG_001480"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8480574.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACHAUG_001481"
     CDS             11787..13820
                     /locus_tag="ACHAUG_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480575.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACHAUG_001482"
     CDS             13864..15144
                     /locus_tag="ACHAUG_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8480576.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACHAUG_001483"
     CDS             15354..16160
                     /locus_tag="ACHAUG_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480577.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACHAUG_001484"
     CDS             16172..17392
                     /locus_tag="ACHAUG_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480578.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACHAUG_001485"
     CDS             17382..18968
                     /locus_tag="ACHAUG_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8480579.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACHAUG_001486"
     CDS             19007..21145
                     /locus_tag="ACHAUG_001486"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8480580.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACHAUG_001487"
     CDS             complement(21513..22496)
                     /locus_tag="ACHAUG_001487"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8480581.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACHAUG_001488"
     CDS             22874..24265
                     /locus_tag="ACHAUG_001488"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8480582.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACHAUG_001489"
     CDS             24281..25321
                     /locus_tag="ACHAUG_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8480583.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="ACHAUG_001490"
     CDS             25340..26425
                     /locus_tag="ACHAUG_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8480584.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26787
                     /locus_tag="ACHAUG_001491"
     CDS             26458..>26787
                     /locus_tag="ACHAUG_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8480585.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYF"
BASE COUNT         8590 a         4449 c         5438 g         8310 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta ttagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca
    26701 ttacaaaatg agaaatattt aaaatatgta ttattatctg gtgctgatta tccaatcaaa
    26761 gataatgagt atatttataa ttatttt
//
LOCUS       DAYYBH010000031        26615 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000031 DAYYBH010000000
VERSION     DAYYBH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.031"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..386)
                     /locus_tag="ACHAUG_001492"
     CDS             complement(<1..386)
                     /locus_tag="ACHAUG_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480586.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKIL"
     gene            complement(571..969)
                     /locus_tag="ACHAUG_001493"
     CDS             complement(571..969)
                     /locus_tag="ACHAUG_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8480587.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(966..1739)
                     /locus_tag="ACHAUG_001494"
     CDS             complement(966..1739)
                     /locus_tag="ACHAUG_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8480588.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1736..2419)
                     /locus_tag="ACHAUG_001495"
     CDS             complement(1736..2419)
                     /locus_tag="ACHAUG_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8480589.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2586..3473)
                     /locus_tag="ACHAUG_001496"
     CDS             complement(2586..3473)
                     /locus_tag="ACHAUG_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480590.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3470..4339)
                     /locus_tag="ACHAUG_001497"
     CDS             complement(3470..4339)
                     /locus_tag="ACHAUG_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480591.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4476..5762)
                     /locus_tag="ACHAUG_001498"
     CDS             complement(4476..5762)
                     /locus_tag="ACHAUG_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8480592.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6035..6613
                     /locus_tag="ACHAUG_001499"
     CDS             6035..6613
                     /locus_tag="ACHAUG_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8480593.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6613..7542
                     /locus_tag="ACHAUG_001500"
     CDS             6613..7542
                     /locus_tag="ACHAUG_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480594.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7539..9275
                     /locus_tag="ACHAUG_001501"
     CDS             7539..9275
                     /locus_tag="ACHAUG_001501"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8480595.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9338..10174)
                     /gene="pheA"
                     /locus_tag="ACHAUG_001502"
     CDS             complement(9338..10174)
                     /gene="pheA"
                     /locus_tag="ACHAUG_001502"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8480596.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10171..10680)
                     /locus_tag="ACHAUG_001503"
     CDS             complement(10171..10680)
                     /locus_tag="ACHAUG_001503"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8480597.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10673..11968)
                     /gene="aroA"
                     /locus_tag="ACHAUG_001504"
     CDS             complement(10673..11968)
                     /gene="aroA"
                     /locus_tag="ACHAUG_001504"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8480598.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11981..13072)
                     /locus_tag="ACHAUG_001505"
     CDS             complement(11981..13072)
                     /locus_tag="ACHAUG_001505"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8480599.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13089..14255)
                     /gene="aroC"
                     /locus_tag="ACHAUG_001506"
     CDS             complement(13089..14255)
                     /gene="aroC"
                     /locus_tag="ACHAUG_001506"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8480600.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14257..15321)
                     /gene="aroB"
                     /locus_tag="ACHAUG_001507"
     CDS             complement(14257..15321)
                     /gene="aroB"
                     /locus_tag="ACHAUG_001507"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8480601.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15354..16376)
                     /gene="aroF"
                     /locus_tag="ACHAUG_001508"
     CDS             complement(15354..16376)
                     /gene="aroF"
                     /locus_tag="ACHAUG_001508"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8480602.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17035..18009)
                     /locus_tag="ACHAUG_001509"
     CDS             complement(17035..18009)
                     /locus_tag="ACHAUG_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8480603.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17990..19873)
                     /locus_tag="ACHAUG_001510"
     CDS             complement(17990..19873)
                     /locus_tag="ACHAUG_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8480604.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19891..22116)
                     /locus_tag="ACHAUG_001511"
     CDS             complement(19891..22116)
                     /locus_tag="ACHAUG_001511"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8480605.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22231..23265
                     /locus_tag="ACHAUG_001512"
     CDS             22231..23265
                     /locus_tag="ACHAUG_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8480606.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23332..24345)
                     /locus_tag="ACHAUG_001513"
     CDS             complement(23332..24345)
                     /locus_tag="ACHAUG_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8480607.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24423..24611)
                     /locus_tag="ACHAUG_001514"
     CDS             complement(24423..24611)
                     /locus_tag="ACHAUG_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8480608.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25051..25179)
                     /locus_tag="ACHAUG_001515"
     CDS             complement(25051..25179)
                     /locus_tag="ACHAUG_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8480609.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25182..26555)
                     /gene="rlmD"
                     /locus_tag="ACHAUG_001516"
     CDS             complement(25182..26555)
                     /gene="rlmD"
                     /locus_tag="ACHAUG_001516"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8480610.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
BASE COUNT         7607 a         5756 c         4725 g         8527 t
ORIGIN      
        1 agaatctttt gctgacgtct aaaaatagaa aaagaatttt cgtcatctat ttgataacgc
       61 atttgttttt gcagttctgc tgcagtcaga tggatcttcc catcgtttgt atcgatcatt
      121 aaaccttctg gaaacagttc aaccactgct cgatcccaag attcaagaga aagctcgata
      181 taatctttta ctggtaattc caattctacc ttaaaaaagt gcaaaacagc cgacagccct
      241 tctgcttgat agatttctcc taatgttagc ccctctagat ggaactctgg tgaaaaatgc
      301 agcaatgttt tattttcctg caattgaaca tatagaaatg tagatcgctc aatcagagta
      361 tcttttaaaa caactaaaaa attcattttt ctcccccttt tttcatcatt tttaaaatag
      421 catattttga aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca
      481 taagtattca cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc cgccttcttg
      541 tttgtgttta tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt
      601 ttcatctaaa agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc
      661 aatgactttg tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat
      721 gccttgacca atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac
      781 aatatctttt tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga
      841 acctcttcca tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac
      901 atcttcgccc attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag
      961 atctttcatt tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca
     1021 tcttttcgtt tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc
     1081 attgcaacca caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt
     1141 gccacgctca ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta
     1201 tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg
     1261 tatcctggga tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat
     1321 cctactgctg cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc
     1381 ccctcgctca attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct
     1441 actaagaacc ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca
     1501 atagcactgc ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa
     1561 aggatcaacc caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg
     1621 cgcatacgaa aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc
     1681 aaaatgactt ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt
     1741 ggaagatcac ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca
     1801 ttccaaaact gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa
     1861 gggccaagat aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa
     1921 tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag
     1981 ccatcaattg ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata
     2041 ctccagtgcc gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata
     2101 tatcatatac ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc
     2161 cgatagcaaa tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc
     2221 ctactagatc tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc
     2281 cgatcatcat gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg
     2341 caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt
     2401 ctttaaaatt ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa
     2461 agagttttcg agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca
     2521 cacgtcccaa aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg
     2581 tttttttatt ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat
     2641 acgatggcag ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt
     2701 gtataggaag cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc
     2761 aaagctacta agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc
     2821 gcggcatagg tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt
     2881 agaccgtcta atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga
     2941 aagaagaaga tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg
     3001 attcccaaaa ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc
     3061 gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt
     3121 tgataaatct caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc
     3181 accaaagcga tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac
     3241 aaattttgaa aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc
     3301 acgggtgtat tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat
     3361 aggatgctta atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct
     3421 tgttttttga cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt
     3481 ctcctcctac tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg
     3541 aaactgctgc cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt
     3601 gagatagggt ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa
     3661 aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat
     3721 ttgggatcgt cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt
     3781 cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag
     3841 tccaacgcca agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc
     3901 agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt
     3961 gagaaaatag acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa
     4021 agatagcggt tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa
     4081 taaaaagtgc tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa
     4141 aaaaagcttt tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa
     4201 atgtcatatt gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa
     4261 taaaaaccat gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga
     4321 ataaatcttt ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa
     4381 gacaagaaag acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga
     4441 tgaaaaagcg aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact
     4501 tgtttttgtg tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga
     4561 gcttcagcta cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt
     4621 tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct
     4681 tcttgataag cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc
     4741 gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct
     4801 gaatcttctc cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct
     4861 tgcgcttttt gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat
     4921 tggttttctg ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact
     4981 gctcctgatt gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct
     5041 tctttcaaaa gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc
     5101 gtgactgtct ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt
     5161 actcgaccaa ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca
     5221 tctcgcgcta cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa
     5281 cctgcttctg ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga
     5341 acaccgtata ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct
     5401 tcctttacaa tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt
     5461 atcctataat cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa
     5521 gcggtgttca gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt
     5581 tcttcgttct cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc
     5641 cacgcccaga cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct
     5701 gttaagaaac ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc
     5761 atttttatcc gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca
     5821 aaatttgaaa acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa
     5881 aaaaatacaa atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa
     5941 tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt
     6001 aaattacaag actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga
     6061 tgatgaacag acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt
     6121 tcaattaata gggacttttt ccagtccgct gaaagcgctt gatttttctt tggtacatgc
     6181 aatcgatatc gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg
     6241 gctattaaaa cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt
     6301 tgatatggtt aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt
     6361 gagtcctgat ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc
     6421 caacaaaagc ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat
     6481 tgctggacaa actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg
     6541 ttatataccg aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc
     6601 acccattcat tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg
     6661 gaagaatccg gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa
     6721 aagtttcgtc aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc
     6781 caagatatca atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat
     6841 cataagggaa agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag
     6901 cagcaagcaa gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa
     6961 gaacttgaga atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa
     7021 catgaggcaa tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg
     7081 aatgaagaac tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat
     7141 ttcgaagatt tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca
     7201 aatgcaaaaa aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat
     7261 tatgagaaag aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat
     7321 ctctcagcca tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc
     7381 cgtatcagta aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt
     7441 gagaaagtcg gctataccga actgggttat ttctccagag tttttaaaga agagacagga
     7501 atgacacctt ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc
     7561 atgaactctt tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg
     7621 tcatttcttt gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat
     7681 cgaatgatgt ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg
     7741 agaatacatt agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc
     7801 cagcccaaca tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc
     7861 tttcaaaaat ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa
     7921 cttttgtcgg aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga
     7981 ttgctcagag agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat
     8041 tgactcttcg cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat
     8101 tgaatcacgt ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt
     8161 ttcaatctgt agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga
     8221 tcatcgcatc aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa
     8281 cagtaggtga aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc
     8341 aggattgtac attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga
     8401 tttttccgat tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta
     8461 tgtatttgga tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag
     8521 aggaagatgg cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag
     8581 ggaatacgat caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag
     8641 acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt
     8701 ttatttataa tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag
     8761 cagttcaagg aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag
     8821 aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta
     8881 tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat
     8941 ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca
     9001 ataaaacgca aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg
     9061 aagtgattga taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt
     9121 tttcaggaat cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat
     9181 atggcttaac gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc
     9241 ttcttcctaa aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt
     9301 attttaagtt aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg
     9361 tcagcactgg ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga
     9421 gcgcattctc tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt
     9481 ctcccaagct agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc
     9541 caaaagctgc cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga
     9601 cactcatttt tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa
     9661 aacgtgtctg gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat
     9721 gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat
     9781 acattgctgc agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt
     9841 ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg
     9901 ttgctggatt gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga
     9961 cttctggatg tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct
    10021 ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga
    10081 tggaagcata cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga
    10141 atgaactttc tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt
    10201 atcgatgatc ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc
    10261 ataccaagaa agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc
    10321 taaaggtcga atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc
    10381 ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac
    10441 aatccctcca ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga
    10501 ctccactttt cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg
    10561 gatatgttcc tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa
    10621 ccctttaccg attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat
    10681 gagcttctcc tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg
    10741 aaacagcttc agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca
    10801 gcatcatacc gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg
    10861 gcccatggat gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt
    10921 cttctgccac cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga
    10981 tgatcgtgat tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca
    11041 gacgcggaat gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta
    11101 aatcagccga ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct
    11161 ccaacacatc taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt
    11221 ggctttccgg aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg
    11281 gaacagttat ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt
    11341 ccactgtgat ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt
    11401 ctttttcaac gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt
    11461 tgacttgcgc actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg
    11521 tcacagtgat tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc
    11581 gtaaaggttc catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag
    11641 tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt
    11701 ttcccatgtc aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct
    11761 tgatcacctc ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac
    11821 tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc
    11881 caaacatgat gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta
    11941 atccatgtat ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat
    12001 aactggtatt agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac
    12061 tgatttgcag tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag
    12121 aaactcccgc ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca
    12181 catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt
    12241 ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga
    12301 tccattctgc tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct
    12361 gcttattgct caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga
    12421 tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt
    12481 ctgcctgatt gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta
    12541 tacggtcatg ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt
    12601 cttgcagttt ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat
    12661 tttcaaatag tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat
    12721 gtccaccgac aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac
    12781 tgctgacatc cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat
    12841 aagctaaggt tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg
    12901 tttcaaaatc acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt
    12961 ttgactgact atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg
    13021 ccatagaagc accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg
    13081 cccccttttt agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca
    13141 acaagcgaat cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca
    13201 ttttccgcaa cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct
    13261 ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg
    13321 ggtttcatga ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg
    13381 cctagattat tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc
    13441 acttctgagc ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag
    13501 gcattgatac taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg
    13561 tagcttccta atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta
    13621 tctccatttt tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca
    13681 acaactcgta cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca
    13741 ggtatttctg cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt
    13801 tcttccagaa ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca
    13861 gaggaccgtt caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa
    13921 tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact
    13981 tctgctacag acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg
    14041 acaggtgaac caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt
    14101 tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg
    14161 atttcttctt ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct
    14221 ggtccatggg attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt
    14281 gaatgtctct gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat
    14341 attcaattgg cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg
    14401 atgacttaga tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc
    14461 tgtcgatcca gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc
    14521 aatagctact ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg
    14581 gccaattgta tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc
    14641 cactactttt cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag
    14701 atctttttcg tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc
    14761 tgatttgatg atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac
    14821 ctcaggatca atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt
    14881 gtttgtattg actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt
    14941 cggaatttga acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc
    15001 acccaccatt cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc
    15061 taaaaaatcg tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac
    15121 gattgaaagg actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata
    15181 tagtggagca acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg
    15241 aagccatgat ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt
    15301 atcaacatgt acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg
    15361 agtgtctgaa tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag
    15421 taagtctttt cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg
    15481 atctctacga tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca
    15541 agttcccaaa ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt
    15601 ttcatcaatg ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga
    15661 atcccacgtt cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca
    15721 ttaagccatt cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt
    15781 ttaccgacag cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata
    15841 tcgctgtact cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc
    15901 attccaaatt catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg
    15961 aaagcatatg gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg
    16021 gctttggcca ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca
    16081 gccatcgtca cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg
    16141 ccaggatgga attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct
    16201 actccttcat agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca
    16261 atcactactc gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga
    16321 tcgacgactt tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt
    16381 tttcccgctt tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt
    16441 ggttttacat taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct
    16501 ttcctactta tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta
    16561 cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag
    16621 atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa
    16681 acctggtttg tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta
    16741 atacgtattc agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta
    16801 agaattgtac ctagtataga cataaaaaaa tcgactgtca atcattttta tgaaactttt
    16861 tttaattttc tgatagttaa ctttgttttt cagaaaaata acaattggtt ttattgaaga
    16921 aaatagtcat gaacaaaagt atctgagaac ttttttactc tcttgttcat gacatataga
    16981 ataaagcagc tttgtcttgt aaactccgtt tttcaaataa taatctaaag attgctactg
    17041 aataataaag ccgatttcat ggtcctcttc gctgctgatt cggtaaggtg actcaacatc
    17101 cgctccccag ctattgatac ccccgactcc tcttactttt gcaaaaatcg taagaaccgt
    17161 tcgtctttct ggcggtagtt cttctatatg tgttgcctgt tccagttctt ctgctttgaa
    17221 tggtaaagcg gaaaaagcaa atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc
    17281 ttgatcttta tccgtattat ccaacgtttg atttcttgtt agttctaccc atttcgtttc
    17341 catatgcatg ccgcattctt ggggaacaag gtatggggaa acggggagtc ctgccacttt
    17401 ataaatacct tcgacaccac cagccatccg atccggatat gtttcccctg ataggccttc
    17461 atactcaaaa aattttgctt tcgttggcat aatgaacctt aaacccaatg cgggtaattc
    17521 cggtaatcct ttttgtccat aataatgcat attgacaagc attttaccct ctccagcgat
    17581 ttggtaggaa attgtcactt gggtttttgg tactgtgttg atttccagtg taaaaatgat
    17641 ttcaaccgtt tcagcgtatt cattacttga atattgatta tttactggag caatcggcaa
    17701 atcgatcttt ttattgtcca ccagtacttc tatttctttg acttttggat acaatccggc
    17761 tcctaaccac ataccggatt taaatccaaa tccgctacct ctgtcattat cggtcgttgc
    17821 tcgccaaaaa actggaagtg tttcccggta tagccattct tttccgtttt tattgagcga
    17881 ttccagtccg cctcttgtat aagaaaaaat ataatgcgta ttccccgatt tgacacctaa
    17941 tgtacagtcg ccgaagacga ttgcaacttt ttccgtatcc atatgacttt tatttactgt
    18001 atttttcata ataatatctt acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa
    18061 cgatcccatt ccctgagaat tcatagtcac ttggtttatc gtcaaagtca ccgccatagc
    18121 gcatcacttc tctgcctgtg attggatcag tcacttggat cgcctgatct ttaaaatccc
    18181 atatatagcc tccttggtac atttcataac gatcaagcaa cgtcatgtaa gaatccattc
    18241 cacctaacga attccccatg tcatgcatat attcacataa gatgaacggt ttttccgctt
    18301 tattttcaag gtagtcaatg atctcttctg gtggcgcata catgcagctt tttacatctg
    18361 aaatcgaatc ttcgaatgct ggatttggaa aattcccttc ataatggaca agtcgatcgg
    18421 ggtctacttt tttgaagtaa tcgttcattt gtcgaatgtt ttcttccgca taagattcat
    18481 ttccaagaga ccaaaaaaga atacttggat ggttttttaa tgtttcgaaa ttcgtttttg
    18541 ctcgatcaag aacagccgcc tgccactgag gtactgcacc tggtacgttg taagaaggct
    18601 caaatgcgcc cattttttgc catgagccat gggattcgag gttcgtttcc gccatcacat
    18661 ggattcctgc ttggtcgcat aaataatacc aagtgatctg gttggggtaa tgagatgtcc
    18721 gtacgccgtt gatctttgca ttttttatga tagccaaatc tttttccata tcttctatag
    18781 tgatggcgcg tccttttttc ggatgccact catgtcgatt taccccgttg ataatcatcc
    18841 gttttccatt gaagtagatg attttatctt tgatttcgat tttacgaaaa ccgaaggaat
    18901 agtcaatata ctccgttact cttttttctt cgtctatcgt ctccaattca agcttatata
    18961 aattgggctg aaaatgcccc cacagtgcca cattttcaaa tccatcgatg cgaatcgtca
    19021 tttgctcttt taacggatat tgctgttgat agatttcctt cccatcagga tctagtatcg
    19081 ctacattcag ctgactgttt ttggctactt gctcaaaaga caaatctact tcaagtttgc
    19141 cattttgaca tttgtcatct aagcgaggtt tgatccaaag atcttctaaa tggttttgtc
    19201 ctcgagcaac taagaagaca tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt
    19261 ccaagaatgc agctgtactt cttttgtaaa cctccacagc tagaatattt tcttcttcca
    19321 aataaggtgt taaatcaaat tcagacggag tgaacgaatc ttcggcatac ccgataaaat
    19381 gaccattcaa ccaaataaac attgcttgtt ctacaccagc aaaatgaatc gagatttgtt
    19441 ttccctttag accttcttct aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt
    19501 tagcagctga aaagatccct tccgcctctt tctcagggct tgtcgtgaaa gggggacggc
    19561 gaaatacatg tccctcccac ggatacatcg tattgatgta atggatcttg tcataatttg
    19621 ctaattcaat atgactagga acagtgattt catcaaaaga acaggcatca aaatcttttt
    19681 tataaaatgt ttttggtcgt accgaaggat ttggcgaata acaaaaacgc catgatccat
    19741 ttaaagactg tttaagggag gatgtggttt ctacttcttc ccttgttgca taacaaacat
    19801 gatcgctatg tgcatttgat tggttcactc taaaaacttg tggatcatct aaccatttta
    19861 tatttgcttt catacttttt tccttctctc tcataacttt ttgaaatgaa gcagttctgt
    19921 ttggaaatca ccttgaaaat cggggaagta aagtccaact gtcatcaatt cggcccccga
    19981 aaatatccgc ttcgaggtct gttcctgata aagtgcctca tcatctaacc ccatcagata
    20041 tacttcatgg aacgctggtt gagctgaagc tagtatcctc cctaaaaaca caatagcttc
    20101 atcttgttta ggagacacaa acatccatgc ggcaatattt tcttcaaaag gactcgccaa
    20161 acgataataa tggccatact ggatcaccgg acgaatcgtt tgataggata taatttggtc
    20221 ttttacttgc ttttcttctt caggagacag ctttgtcaaa tccagttcat aaccaaaaac
    20281 agcgctcata gctgtttctc ctcttgtttt caacgaagta gatctgtcgg tctgatgatt
    20341 tggtacagct gatacatgag ctgtgatgga cgaaatcgga taagcaaggc ttgttccata
    20401 ttggattttc atacgctcca ctgcatccgt gttatcactt gtccatgact gcggcatata
    20461 ataaataaac ccggcatcaa accgtccacc tccaccagaa cagccttccc ataagatttc
    20521 cggataatca gttgtcaaat gttcaagtag ttcataaagc ccgagcatat aacgatgcgc
    20581 tacttctcct tgtctctgtg ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt
    20641 catatcccat ttgatgtagt caagaggaat cgtatctaaa atctttctca tttgtttctc
    20701 aatcgtctgc cgaacatcta accttgtaaa atccagtaca tgttgtgatc tggaagcaga
    20761 aggcattcta cctggctcct gcataagaaa atcaggatgt gttcgataca gttcagaatc
    20821 aaccgagatc atttcaggtt caaaccataa accaaatttc aatcctttgc tatgagcata
    20881 atctgctatt tcacgtaatc catttgtcaa cttgccttca tactcgaacc agtctcctaa
    20941 tgaagaattg tccgcatctc tttttccaaa ccagccatca tctaatacga acatttcgat
    21001 gcctagctcc tttgcttcat caatgatcgc cttgattttt tcactattga agtcaaagta
    21061 agtggcttcc cagttattga ccagaatcgg ccgttcagca aattgatgac gacctcttgc
    21121 gattctttca cgtagcaact catggtggat ctgactcatt ttgttcaatc cctgacttga
    21181 gtaagaaagg atcatttcag gtgtttgaaa agactggcct gcattcaatt cccacgaaaa
    21241 acgatggctg ttgatcccac cgacgactct caattgattg atctgatctt tttcaagttc
    21301 gaaagaatgg ttgccggaat aaacaaattg aagtccgatc gcttctccag tattttctgt
    21361 cgtatgatga tgaactaaag caataaagtt attcatatgg tgactgctcg ttcctcgacg
    21421 gctttcaaat acttttgttc cagataaaac agattgccgt gaaatctgtc tttcattgac
    21481 atgtgctcct ggcaaagcaa tgacttcatc aaatcgccta gtatgggcaa agtctagctg
    21541 gaaactagcc gctttttcta gtttgattgt ttctccggtt tcatttctta tctttacaga
    21601 tcgtgtgatc actgctctat cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt
    21661 ttctctatcc ttaagtgaaa tcaccaacgt ttgtgcctct gacttgtcca tcacataagt
    21721 ttgaggcaag ccttcgatct ctggcttccc atctgtaata taatggctgt catatctcag
    21781 atccagtaag tctgatccat tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat
    21841 gctgccatga gaagagtatt cttgcggtag tgtatctttc gacagagacc ggtcaggatt
    21901 taagggaaca tttcctgaaa atccacgatc tcttcgggga taatttttat tgtctttgta
    21961 ctgcttgact ggtttgccaa aataaatatg cgccaataca ttgctttctt ctatctttat
    22021 cagataagaa agaaatgtat ttcgtaaatg gaagactcgg ctcttttcat caaataagat
    22081 gaaattttta tctgtttttc tatgcgtaat atccatcatt tcactcccaa caaaacggaa
    22141 accgtttact tttttttata attcctgtat actgtttact aaacaatttt gtcaataaaa
    22201 attttttatt tttttgagga gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag
    22261 gcaaatgtct ctcctgctac agtttcccgt gtgttgaata acgatcagac gatccatgtg
    22321 gctcagacta ctcggcaacg gatagtagag gcagctgctg cgttggatta tgacatcagt
    22381 actcgtaaat atatcaaaaa gagaatgcct tcgattggtg tgatctctac gatcggcaaa
    22441 gcgagcgaag aacaggatat ctattacaaa catttgcgag tagggatgga agaagaagct
    22501 cgacgtctgc atttagggat gaatcgaatc tataatctat ctgataatcc taaacaatgg
    22561 gaagatctag atcagttagg tgcggtgatc gttgttggga ctgttacgaa agaatcgatt
    22621 accgccttgt taaaacaaaa catgcaaata gtcgtcgtgg ataaccctga catccagcaa
    22681 gaggtcgata tggtgtatgc agatcttgaa cggatgacta aaaccgtact ggaattgttc
    22741 ttagaaaatg gtcataaaag aatagcctat atcgggggat ataaagtcga tatggatgaa
    22801 ttcggtggga agcagatttc tataaatgaa aagaggatgc gtgcctatca gcattttatg
    22861 atcgatcatc acttagaaaa atatatgtct tatcatctag gtggctggac agaaaaacaa
    22921 ggtgaggtac ttgcgactaa tctattagaa aatgaatccc ctcttcctac tgccattctt
    22981 gtcgggagcg atccaatggc aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa
    23041 attggagaag agattgttgt agcaagcttt gataatatcg agacaatcgc aaacctctcc
    23101 cctggcttga ccagtgttca aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg
    23161 gcattggagc gaattgacgg tatacgggac gaaccaatca ttttgactta tcctgcacga
    23221 ttgattgtta gggaaagttt tatcccaaga acaaaaaggt attgaaagag aggttgtgaa
    23281 aagccgcttc gtttcttcat actcaaaacg aaacggcttt ttcacaacct cttacttata
    23341 cttattcaca aaatccaatg tctgctggta acaattttgc gattctttta gcgtgtaatc
    23401 aaattggtat tcatgggtaa tctctttttt tgtatctgta taaaacaagg attgcacggg
    23461 tacctttaat tctgtcaacc gattttctaa tgcttgacct tgctcctgga atgagtatgc
    23521 atttccgtca gttatatacg ttggcggaaa ttccttagaa acatggtcag ccacactagc
    23581 ttctttcaat tgctcactcg ttttccaatc ttttgttcca atcaacgacc acgcaacggt
    23641 tttgacgaaa aacttcataa atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc
    23701 aacaggtccg caataagaaa tggttccttt cagactatct tttggaagta gctgtttgat
    23761 tttcatttgt ttagcatacg catcattcgt ttgtatagct gcatattgca atgcgatttg
    23821 tgaacctgca ctatctccac caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt
    23881 ttcttcagct aataaatctt tgatcagttc attcacttga agaacttgat tagggtattc
    23941 tgaatcaggc gctaattcat aattcatcgc aaccactgcg atgttggcgt ctgaaactaa
    24001 ttttgtcgca aattctttga cacccgattt atctccgccg acgaatcctc caccatgtac
    24061 ccaaaataga acaggaacag gccccttgct gttttttgga tagtagatgt cataagtatt
    24121 ctctttatac ttggactcat acgtcaaatc tgtttttttc gccacgtttt tttctgcttg
    24181 atcgaacgtt tttttatcag tgatttgaac agtatttgaa aacatatggt tgatcaagaa
    24241 tgctccaggt ctgggggaaa gctggaaggc aagaaagatt gcaaacgcga tcccaagaat
    24301 gattccgaag aaccatccga taaatttaag aattttcttt ttcataaggc tccctcagta
    24361 gttagtatta agcattctga ttataccata gtgttatttc ctatcaagct ctcctagatg
    24421 atttaatgat tttccccaaa cttcttctgc aatgcttttg acaaaagaaa ttttttccat
    24481 tgttcttcaa acaaaacatt ttcttcttct gttgaagcaa atccacattg aggacttaag
    24541 cacaaacgat ccaaaggcag atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg
    24601 tgctcttcca tttcgccatt tttcgttaca gtcaaaatct aagacaacta atttatcttc
    24661 agagactttc tctaaaagaa caaaactccc agcacgttca ctatcgtatt ctaaataata
    24721 ggcacggaca ttttcttgag taaaataatg ggtctgctac gtcgtcatcc cccctgatgc
    24781 cgccaaaatc gaataataat ttccgcgtca aacatgtata tttagtgttg aatcatttga
    24841 gaatatgaaa ataaaaatct ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat
    24901 ctgagacaga atgacgttca ttctgaccca gatttgattt tgcacaactc aacagtcctt
    24961 attggcaaat cgccaaaata atgtagacta actaaaaaga ctaagaaaca ataacaaaag
    25021 ttaacacttc ttagtctttc tttagcacgt ctatttatta aagattttag ggccttttct
    25081 catgttttta cgatcattct taggtgtttc catttgtttg gatcggcctc caccttgttt
    25141 ctttttgatc aattctttca ttttttcttt ttggtccatt tttattctcc tttaacgaat
    25201 gaagcgacta attcgatatg atgggtttgc gggaaaagat ctacaggctg aactttcttc
    25261 acggcataac ctgcttctgc aaataactta gcatctcttg caaaagttgc cggattacat
    25321 gagatataga cgattcgttc tggtgacatt tcgattgctg attcaataaa atgatgatct
    25381 aatccttttc gtggagggtc aactacgatc acagatggtt tgatcccttt ttcttgccat
    25441 tttttcataa ctttttctgc ttttccgact tcataatgaa cattttcgat tttattattt
    25501 aaagcattga actgtgcatc ttcaattgct tctggaataa cttccatccc atacacctta
    25561 tccactctgt ctgctaaaga caagccaatc gtacctattc ctgaataagc gtcgatcaca
    25621 atgtcttttt tatctaactc ggcaaactcg atggctgttt ggtacagata ctctgcttgt
    25681 tgagtattta cttgataaaa tgactttgct gagattcgat aggtttttcc taatagctga
    25741 tcttcgatat agtctctgcc atataaaact ttttctttgt ctcctaaaat cacatttgtt
    25801 ttttcttcat tgatattttg gatgaccgaa accacttctg gcaattcttc atgaatagct
    25861 tcagcaattt ccttggattt aaagaatttt tctttacgag taactaaaac gaccatcatt
    25921 tcatgggaat aatgaccacg acggataatg atgtgacgaa gctgtccttc atttttttct
    25981 tcattatatc ctcgtacttg gaaccgttgc aagatatctc tgactacttt gattgcttga
    26041 tcgatgacag gatcttggat ataaaaatct tcgataggga ctagatcatg gctattcttt
    26101 ttgtaaaaac cggtggctaa ctgtccatcg atacggcgaa cagggatctg tgctttatta
    26161 cggtagccga atggttctgc cattccgacc gttggaagta caggaacttc tggcatctta
    26221 gcaatcttcg ccattacatg ctctacttgt ttttgtttga agacaagctg ttgttcatac
    26281 ttcaaatggg ctaaaggtgc gatacctatg cgaagtaatg cattgttttc tacaggctga
    26341 cgatcaggtg attgtgtaat aaatgtttcg acttttccat atccaaactt attgcctgct
    26401 tttactacta gtatttctac ttcttctcca gggagtgcat tttcgataaa taatggataa
    26461 ccatcgattt ttgctacccc cattccttca taactcaagt cgacgatttc gactgtatat
    26521 cgttcgtttt tctttactat ttgctgttct gtcatatttt gctcctttaa gcctattttt
    26581 caatcctact gaactctact ataaatcagc taatt
//
LOCUS       DAYYBH010000032        26584 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000032 DAYYBH010000000
VERSION     DAYYBH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.032"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..1920
                     /locus_tag="ACHAUG_001517"
     CDS             226..1920
                     /locus_tag="ACHAUG_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFM8480611.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            2608..4485
                     /gene="ltrA"
                     /locus_tag="ACHAUG_001518"
     CDS             2608..4485
                     /gene="ltrA"
                     /locus_tag="ACHAUG_001518"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8480612.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            4616..4993
                     /locus_tag="ACHAUG_001519"
                     /pseudo
     CDS             4616..4993
                     /locus_tag="ACHAUG_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            4993..6198
                     /locus_tag="ACHAUG_001520"
     CDS             4993..6198
                     /locus_tag="ACHAUG_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8480613.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            6214..6858
                     /locus_tag="ACHAUG_001521"
     CDS             6214..6858
                     /locus_tag="ACHAUG_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480614.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            6869..7675
                     /locus_tag="ACHAUG_001522"
     CDS             6869..7675
                     /locus_tag="ACHAUG_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480615.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            7697..7927
                     /locus_tag="ACHAUG_001523"
     CDS             7697..7927
                     /locus_tag="ACHAUG_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480616.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            8473..8748
                     /locus_tag="ACHAUG_001524"
     CDS             8473..8748
                     /locus_tag="ACHAUG_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480617.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            8790..9212
                     /locus_tag="ACHAUG_001525"
     CDS             8790..9212
                     /locus_tag="ACHAUG_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480618.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            9231..10823
                     /gene="mobP2"
                     /locus_tag="ACHAUG_001526"
     CDS             9231..10823
                     /gene="mobP2"
                     /locus_tag="ACHAUG_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFM8480619.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            10835..11170
                     /locus_tag="ACHAUG_001527"
     CDS             10835..11170
                     /locus_tag="ACHAUG_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480620.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            11317..11511
                     /locus_tag="ACHAUG_001528"
     CDS             11317..11511
                     /locus_tag="ACHAUG_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480621.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            11543..13915
                     /locus_tag="ACHAUG_001529"
     CDS             11543..13915
                     /locus_tag="ACHAUG_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8480622.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            13976..15436
                     /locus_tag="ACHAUG_001530"
     CDS             13976..15436
                     /locus_tag="ACHAUG_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8480623.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            15447..17651
                     /gene="topB"
                     /locus_tag="ACHAUG_001531"
     CDS             15447..17651
                     /gene="topB"
                     /locus_tag="ACHAUG_001531"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8480624.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            17667..17816
                     /locus_tag="ACHAUG_001532"
     CDS             17667..17816
                     /locus_tag="ACHAUG_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480625.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            17890..18483
                     /locus_tag="ACHAUG_001533"
     CDS             17890..18483
                     /locus_tag="ACHAUG_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8480626.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            18499..19398
                     /locus_tag="ACHAUG_001534"
     CDS             18499..19398
                     /locus_tag="ACHAUG_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8480627.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            19401..19886
                     /gene="ssb"
                     /locus_tag="ACHAUG_001535"
     CDS             19401..19886
                     /gene="ssb"
                     /locus_tag="ACHAUG_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8480628.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            20252..20512
                     /locus_tag="ACHAUG_001536"
     CDS             20252..20512
                     /locus_tag="ACHAUG_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480629.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            20953..21159
                     /locus_tag="ACHAUG_001537"
     CDS             20953..21159
                     /locus_tag="ACHAUG_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8480630.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            21159..21410
                     /locus_tag="ACHAUG_001538"
     CDS             21159..21410
                     /locus_tag="ACHAUG_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480631.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            21425..21862
                     /locus_tag="ACHAUG_001539"
     CDS             21425..21862
                     /locus_tag="ACHAUG_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480632.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            21855..22562
                     /locus_tag="ACHAUG_001540"
     CDS             21855..22562
                     /locus_tag="ACHAUG_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480633.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            22727..22927
                     /locus_tag="ACHAUG_001541"
     CDS             22727..22927
                     /locus_tag="ACHAUG_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480634.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            22943..23122
                     /locus_tag="ACHAUG_001542"
     CDS             22943..23122
                     /locus_tag="ACHAUG_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480635.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            23463..23726
                     /locus_tag="ACHAUG_001543"
     CDS             23463..23726
                     /locus_tag="ACHAUG_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8480636.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            23720..24070
                     /locus_tag="ACHAUG_001544"
     CDS             23720..24070
                     /locus_tag="ACHAUG_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8480637.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(24094..24708)
                     /locus_tag="ACHAUG_001545"
     CDS             complement(24094..24708)
                     /locus_tag="ACHAUG_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8480638.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            25022..25444
                     /locus_tag="ACHAUG_001546"
     CDS             25022..25444
                     /locus_tag="ACHAUG_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480639.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            25454..25657
                     /locus_tag="ACHAUG_001547"
     CDS             25454..25657
                     /locus_tag="ACHAUG_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480640.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            25868..26452
                     /locus_tag="ACHAUG_001548"
     CDS             25868..26452
                     /locus_tag="ACHAUG_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8480641.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         9884 a         4239 c         5457 g         7004 t
ORIGIN      
        1 ccactctaca aaaaagaatt gatcaagatt ctgtatcgaa tgaacaatat gaatgatgag
       61 taggaatgga gaagagagaa tgaaatatcc gttgatatgg ttagaaaaag atgttccgaa
      121 ggaagagacc actagaaaat ttcctcacac tttaagcctg ggtaagttgg gaaaagggca
      181 atcttttaac caagtaaaga atcaaacaag aagaggtgac tgtacatgtt agaagcaatt
      241 aaaacagctt tttcaccaca aaatgatcaa aaagtacttg atccattaaa gcaactagga
      301 aatcgggaaa tagaaattgt aaaatccaaa gggtatgatt tagatttttt aaaacaagtg
      361 atgccacaag gtgggttaaa ttttgatgaa gaatatgtga cgtatggtaa tggttattca
      421 cgatgtgtga cagtttatca ctacacaaaa aatcctacgc ttttttggtt ggccaatctc
      481 atgaacaatc ctcatacagt ggccacactc gatgttagca cagatgaaaa gcaaaaagtg
      541 attgatcaaa tcaatcgtgc cttagatgaa ttaaatgatc gatcagataa tgaacgaaaa
      601 gcgactgacc gaaataaaag cgcggaagaa tacctggaac tagcggaata tgctagaaaa
      661 ttgacttcta atggggaaat ttctaagcaa gtcaaagtga gagtacatgt ttacggaaac
      721 acgattcaag aagtagatga acgagcgcag gaactgatcg aaaatctcaa agggatggat
      781 tatcgtgcgg tgacttatct gcataaaacc aaacgagatt ggcaatcgat gtttaccatg
      841 ttcgatgaac aagctgaatg gttcggaaat atcaaagggc aatcactacc atcgttaaac
      901 attggcggag gtttcccgtt ccaccaccaa acgctaaaag atccaggagg gacacaatat
      961 ggggaaacca tgacaggcgg cccatttgtc tggaatgcga cgtataaaaa tagtgtacgg
     1021 ttgtctacta ataccttgct tctaggaatg atgggagctg gtaaatcgac cgttcttaaa
     1081 atgatcgcgg aagcccattt agctgcagga gactttattt ggggctttga aaagggaaaa
     1141 gactttattc ctttcttaaa ggaatataac ggtattatgg tgcgtttaga cggatctgac
     1201 ggaatgatca atcctttaga gatatttgcc acacggacgt atgatgaagc atccagttta
     1261 tatgacgatg gatcagtcaa agatttgaag atcaatgaag cagcgtccta tcaaacacac
     1321 ttagacaaag tggtctatca ggtacagtta gtgagtcctc agctaaaagg aacgatgaaa
     1381 gcagaattta agacctactt aaatcgtttt tatgaagagt atggaacagt accacgcggc
     1441 tttacttcct caaattcgcg ttcaaatact gatacgcaag tcacagggaa agatccagaa
     1501 gcttatccga cgttcaaaga atttttagac tttttaggac aactggaatt accaggtgct
     1561 tcccaagaga aaaagaatcg aaaagaagaa atggaatcca tcgtagaatc cttatgtgag
     1621 acgtatggca tgatttttga tggtcatagt acgattcgac accttgacca acaacagttg
     1681 gtcttttttg acattgactc tattacaggc ttagatcgag gggtacaaca atgtcaaatg
     1741 tacatggcta tgacgttgat ttggaatcag gcattgattc aaggaagaag acaacgacgt
     1801 ttaattaaag aaaaacaaat ggaagaacaa gacttcaaac gcttcttggc gatgattgat
     1861 gagtgccata atggtgcgac ctgttctgca gtgaaaagct gtgggttgca agtagtctaa
     1921 aaactattgt aataactctt ataacgagaa gtcaaatgag tgggtaacgc cacaaagggc
     1981 ttccactgat actccgacat gcaacaagta gtttatgcag accaacgttg tgtgaagctc
     2041 ggtgaagtcg gctgaaggat accgtaaggt aaaagaaata gtgaatacca cgaaagttct
     2101 ctagaggtaa agggtgtatc cgataagtcg ggtgtccaaa aaaacttaat atggtgagta
     2161 tgtacaaacg tactgacgaa ctagcgaatg tactgtccga aaggcatagc tcctagaaat
     2221 gggagagaca gtgagcaaaa aatcactcat tgtgctaact tgtggttgag taaaagtcgt
     2281 tgttatgaaa gaccatgctg cgttacaggc gtaggagaag ttagggggca aaagctagca
     2341 tctaagttaa ggcatgtgga tagatataag ggaacagggg aagccatcaa catggagagt
     2401 gcatactcga tgaagtggta gtaagaaata tgtgaatgac aaataactta ctttagtgat
     2461 ggtgaaagta gagtcacgac tgatgaaggt tctgtaatgg aactggagga acaggctcga
     2521 ttcgtctgta aaagtcatga aacccgttca accatgggat tctcgtatga ctaaaaaggt
     2581 cacgagccaa ttggagcgtg ataaaccatg acaaaagaaa caagtccaaa agataggaag
     2641 ttgcggcatg cagaatatta tggcatgacg gaaatcttcg ataagatata ctccaaaagt
     2701 ttgaagggag ataatttcaa gaatctaatg cagattatca tttctgataa caacattgtc
     2761 ctagcatatc gtaatatcaa gagaaatcac ggtagtgtga caagaggaga agatggagta
     2821 acgattaaag atattgaaaa tctcaaccgt aatgaattcg tagaaattgt taggaagcga
     2881 ttcgcacatt ataaccctcg gaaagtaagg cgtgtagaaa ttcctaaata taatggtaaa
     2941 ttaagaccgt tgggtattcc atcaatatgg gatagaatcg cgcagcaatg catcttacaa
     3001 gtattagaac caatatgtga agcaaaattc aataatcaca gctatggttt ccgcccaaat
     3061 agatcagcag aacacgcaat agcagattgt atggcccgaa tgaatcaaag tcacatggaa
     3121 tatgtggtag atgtagatat caaaggtttc ttcgacgaag tcaatcatac taaactcatg
     3181 agacaaatct ggtctatggg tatacgagac aaacagcttc tcttaatcat taggaaaatg
     3241 ttaagagcac ccgtagtact tccaaatggg aagttgatat acccaacaaa aggaacacca
     3301 caaggcggaa tacttagccc attacttgcg aacatcaatc tcaacgaatt tgactggtgg
     3361 atagcaaatc agtgggagga aaaagatgcc aaagagctaa aacatcattt taggaaaaat
     3421 ggagcttggg ataaaggtaa tatctatcac aagctcagaa agagtacaac tctaaaagaa
     3481 atatacctag tgagatacgc agatgatttt aaaattttct gtagaaatta taatgaagcg
     3541 aaaaaagttt tctatgcaac taaaatgtgg ttacaggagc gtctaaagtt accaatatct
     3601 gaagaaaagt caaaaatcac aaatctttca aaagaagaaa gtgaattttt aggctttacg
     3661 atgaaattag tgaccaaagg taacaaaaag gttgttaata cgcatatagg tacaaaagca
     3721 caaagcatga ttaaagtgaa actaaaaaaa caaatcaaac gaattcaaaa aagtcctaat
     3781 agcaaagata cctttatcta tattggagtc tataacagta tggttattgg gattcataat
     3841 tacttcaaaa ttgcgactca ttgtagtgta gcaatgcgaa aaattgcaag agaactcaga
     3901 ccaagttttg aaatacgact acgagaacaa ggaatctcaa aagaaggaca atacggaaag
     3961 tacgaaggat acaagcctta cctacggtca ggacaaatac gatatataag agggtttcca
     4021 atattaccag tagggtatat tcaacacaga catccgatgt gtaaaaaaag aaatattaat
     4081 aaatacaccg aagaaggtag ggaagccatt cataagaagc aacaaaccgt agaagaatgg
     4141 aagatatcgt ggttgtgtag caatccagtg cttagtaaga gagcaacgat tgaatttgcg
     4201 gacaatcgaa tttcaaaatt catcgctcaa aagggaagat gtgcagtaac aggtgaggag
     4261 cttatcttat ctgaaatgca ttgtcatcat attatccctt atcatgaaag caaaagtgat
     4321 agctatgaaa acctagtgat tgttactgag gaggttcatc gggtaataca cgctacacaa
     4381 agtgaaacga ttgaggaact tctaaaatat ctaaaattaa atccaaaaca aaaagaaaaa
     4441 ctcaatgagt tgagattaaa agttggtaac gaagaaatat cttaataaat atttgtagga
     4501 cgggaaaaca gaatgtcttt tatagacgag ggaacgccgt atgcggtgaa agtcgcacgt
     4561 acggtgtgga ctgggggaaa agacggagat aatatcaaac tcttacctat cagtatcgat
     4621 taatccagat tttattgaaa cggtaaatta tgtcacaaac tttcaacgag aaatgcgtaa
     4681 agtagaagca atgactgttt tagctacaca atccccacaa gaaatggtgc cagaaaatat
     4741 gtcttctgat aacttctcga aactgaagaa agtctttgaa ttttcttcta cgaagatctt
     4801 tatgcgtatg gatgaatcta ttttacgtca tatagaagga ttagtgggta aatcaatgac
     4861 gaactcagaa ttagaatcca ttcctcaaca aaatcaagga gacgctgtga tcaattttgg
     4921 ctcaaaagaa accattcgtg ttcattttag tccgaacaaa cgtcaattaa aattgtttga
     4981 tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca agcagtatgt taagcgtcgt
     5041 gcaaagcggc gcttattttt gtggttgttt ggtacttctg caggtttgat tactattttg
     5101 atcactgtgt tcgtgaccct atttctaata cttgcggcgg gctcgattga taattcggat
     5161 tcagattcaa gcagtggtgg cgaagctttt acaggagaat attcggaagg cttaccgatt
     5221 tataaagaaa tcaaaggaag aggccctttt tctgatgaga ttgcgcaata tgccgtaggt
     5281 gcagcagtga aatataaatt attaccaagt gtgatccttt cgcaatacgg ttacgaatca
     5341 gcgtttggta catcggcgtc cgcaagaaat gatctcaact attttgggat tacttggttt
     5401 gatggttgtc tgtttcctaa aggaactgca cgaggaattg gtggcattga aggtggctgg
     5461 tacatgaagt ttcccaactc aaaagctgca ttttcctatt acggctttat ggtagccaca
     5521 caatccaatt ttaatgcttg tgtaggaaat aaaagccctg gcgcaagctt attgatctta
     5581 ggacgaggcg gttatgccgc tgcaggaatt acggaagata gtccgtatta tacaggttgt
     5641 atgtcaatta tcacttctaa taaattaacc gaatatgatg aattcgccat caaacattgg
     5701 ggtgaaggtg gcaataacaa cggcacgatt actggtgaat ggacgaatcc attccctggc
     5761 agcagtttag ataaaagttc cttcagcggt ggtcaattat ttggaacgaa tccaggcggt
     5821 gaattccgtc cgaacggctt ccatgatgga ttagattttg gttcagttga tcatccagga
     5881 agtgagattc atgcggttca tggcggaaaa gtcgtgtacg ttggcaatcc tggtatttca
     5941 ggtttgggtg cgtgtgtgat cgtgattaat tacgatggct tgaatatggt gtatcaagaa
     6001 tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg atcaagtgaa agttggtcaa
     6061 gtaattggga tacgagatac agcacacttg catttaggat ttactcgaat ggattggcgt
     6121 caagcacaag gacatgcatt cattgatgat ggtacatgga tcgatccgtt accatttttg
     6181 aatagcagta aaaaatagat caggagaaaa aatatggaaa ataaaacgtt aaaaatcgtc
     6241 ttaacagctg caattagcgt tagtttaatc ggcaatattg cgttgttttg gcagctgaaa
     6301 gaggaacaag cagcaaatac ctataaaatt gaagaagtgg ctaaaaacgc gtctgagaag
     6361 ctcaaaaaaa attcctctga ggcacaggcg aagatcgaca agttaaatca aaccatcaat
     6421 gagaaaaacc aagaaatcga tcagctgaag caacaagatg gcacaaaagc aaaagaggaa
     6481 gtaactgaag cacaaagaaa gacggcagaa gagttcggtc aattggccat tgataaatca
     6541 ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg caacccaaga agtgatcgac
     6601 aaattaagtc ctacagacga tgatcaccaa catgatgatt acggaagtta ctcctttaca
     6661 ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa aagaggaaca aaattttgtc
     6721 gtatttgttg attatacaat cggcaatccg caattcaaag atgtcaaacc acaaaaaata
     6781 aaaggtggat taactgtcac tgaaaaacaa gtagatggtc aatggaaagt catagacttt
     6841 agttatttca ctcgttagga ggaagaaaat gagtaagaaa aaaggagaaa gacaacgttt
     6901 caaaaaacga aaaagttatc ttagatggct gggaatcttt atgattctcg gctttttgat
     6961 tcttgctagt ccgatttata cgaatcagaa ggtagatata ccagatgagg taagcacgaa
     7021 cgccttttta aacacgaaag agctgacaat ggtttcaaaa gaaaaaggag cgaaccagca
     7081 attagttttt gaatttgcga tcgatgatga agatcaaagc ttgttaaaag aactggctaa
     7141 tcttaattat cgcgtagaag taaagacagc taaaggggat tatcaagcaa tacgagccaa
     7201 agtgattaag gtgtctgatg attattttgc agtgaagcta acgcatgtac cagaagaata
     7261 tatcgccatg cgtttaacca ttattcctga aaaaattgat ccaaaagtgg atatgcaaga
     7321 accacaagat ctgatttttt atattcacga agataaggtc aaagatcacg tgaaagacga
     7381 agattatgaa caacatgcga tcaattacaa agtgaaagga tacaagaaag aacaaaaagc
     7441 agctcaaaaa caaatggaat ctttccaagc aaccattgat ttgaatgaag aattagtgaa
     7501 gaagctaaaa gatcaattgc cttatcaagt cgcagatgat caggaaaata ctgaaaacaa
     7561 gattaatagc taccagcaag aaattgaaac atcgaaaacg caactgaaag atcaagaaga
     7621 gatcattcaa aaacttcaag aaaaaattga tcgtatgaca aaagaaaaga tttagaagga
     7681 caggtggagt gaaaaaatga agcgaacaaa cttggctgac aaagatactt tcgctgagca
     7741 gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat gtcgagaagt
     7801 gaatttatct aacacaacaa tatctgagtt gaagaatggt cgaaagaatt tagatacctt
     7861 aaaattcaga tcatttgaag ctctatatgt agctgctaaa aaatatttta cagctgataa
     7921 caaataaaaa acaggagtat tttatgtgaa aaaagtatat aaaaaatgct tatgaatcag
     7981 ctatatcacc ttaaccattt gttaatgaga tgggagaaaa cattaaccac gattctaacg
     8041 caaacatcaa gcacataaaa catttaacac tcctcctttc tggctgaaga tttcaaccaa
     8101 acaggaagac agcgtatcaa ttatagcata cactttttct taaattcatt tttcatatga
     8161 caaaatattt ccttctaatc gacaaagtta gaaggatttt ttattgtcta aatacacaaa
     8221 taaccttaat ttttaaagag cataaggaaa cgtgatatca ctttctcacg aaaggtgtta
     8281 ggtaggctgt gcctaccacg tttctaagaa agataaatga tatcaaaatt ttggatttgt
     8341 gtgtagattt gtgggtagga tgaaagcgaa tgtgggcagt aaaataagcg tttgtgtgta
     8401 gcaaaatcaa aaaatgtgta gggatatgtg tagctacaca tatccctaca cataataaat
     8461 ttggaggaaa atatggactt attaattaga aatattgaag aaaagtatat taataaaatt
     8521 gacaaacgtt gtgaagaact tacggtcaag actggcaaaa aatggagtcg aaatcaatac
     8581 ctaaaagttt tgatcgaaaa tgattttgat cacgctcttc tgaattataa aaaagaccaa
     8641 tttgatcggt tgttagaaaa atttgtggat attcaaactt ataatacgaa aactttagaa
     8701 gaatatattg caacaaataa tcaattaatt ggtttgttaa ttgaataata aaaatagtat
     8761 tgtgaattta taaaaggagg aaatcaattt tggcaaaagc aacaagaaaa catgtatatc
     8821 tttacgatat gcatatcgaa gtacttgatg aaataatttc tactcatgaa gatatatcta
     8881 attatagtga agcagtcaga catttgtgta tgatgtatga tccaaaaatc gaagaaaaaa
     8941 taaatccaat tcaaattaaa ttgaatgcaa tgggaaaaga aatttctatt ttaaccgaaa
     9001 tggtttcaga gtttgcggat cttcatttga aagatacagg aatactaatt ggtacaaatt
     9061 caactgttta tcaagaagca gaagaacgtg tggaaggaaa aattaaatcc aatcaaacca
     9121 aaaaatattc ttctaagtta aagaaaccag tgaatgaatc tgttcatacc agagtagatc
     9181 catttgatcc aacgaagatt ctaaaaagat aaaagtagaa ggagaacgtg atgaataaat
     9241 cgcctgcagt tattcttact tgtcaattta ccttgcccaa taaaaaatct ttttcaacat
     9301 acattgatta tatgacaaga gaaaaagcac tggaagaaat ggaacgacgt acgcccgaag
     9361 aagaacagga actgcaacga atcaagaatg ctttagagga tttttatatt cctgaaggag
     9421 aaactttttc ggaaagtaag acggataaag aattcagtga aacgtcgaat gaagctcaat
     9481 cgttgatgga acatggaatc aattttgaca agttagagga acaagatttt accaaatatc
     9541 tttcttatat gacccgtcac tatgcgttag aacaaaagaa agaactgacg caaagggaac
     9601 aaaaagaata ccaacggttg aaccaagcaa tgaaaaaata tcaaacagag ccacataaaa
     9661 aagcttcagg aaatcttcct ggagtttttt ctatggcttc agacgaagtg aaaggaagtg
     9721 atctgaagga gatcaaacaa atcttccaaa gaggacaaca aaaaggctca atcatttatc
     9781 aagatgttat ctctcacgac aattcgtatc ttgaaaaaat gggcttgtac gatcccaaaa
     9841 cagatacctt agatgaagaa ggtttgaaag cagctggaag aaaaatgatg gagacattat
     9901 tcgagaaaga acaattgaat gaaacaggtt actggatggc aacgattcac cgtaacacta
     9961 agcatatcca tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa ccgtatactg
    10021 agattgaaaa tggtgtgagt tatatcgtac cgaaggggaa gagaagacaa gcaacattag
    10081 acgcaatgaa aaccaattat gtacatgagt tagaacgttt ctcttatgaa aaggatcgaa
    10141 tgatcggtat ggagaaacga aatttattga cacgaaaaag tgatttgcgt aacaccttaa
    10201 caaaagtagt aaaagaacca gcacgttacg atcgacacgc catgaactta ttaaatgaag
    10261 tatataaaac cctacctgaa aaacgttcgg aatggaatta tggagatgaa aatcgaacca
    10321 agctttcacc atttacaagg ggaaaacttg atgagttaac caaatatgtt ttaacaaaag
    10381 aacctggata tcaagaatat atacagattt caaaagcatt gaaagaagaa gccaaaagcc
    10441 tttatgggga aagcaaaaga gaaacaaaga atgcagaaaa aaacgccctc tttgatttaa
    10501 aaaagcgcac aggaaatgcg attttaacag aactgaagag acaggatagt caaataaaac
    10561 cattaataga aagtgtcaat cagatgcaat ctatttacaa tgaacgatcc tttcgaacac
    10621 tcaaacagtt gccaacaaat gaatacaagt atagttatca agaattcgta aagcgtagga
    10681 aacaattcca acaaagagtt gtcacgcagc gaagcttgcg tcaattaaat cgaacaatta
    10741 atgcacatga agaaaaatac cacgcaagta aagcatatga agagcaacag cgaagaatcc
    10801 aaatggaaca agaaagacaa taggaggaag agaaatgaaa caatatttag atcaatggaa
    10861 agtcatagaa ggatcactaa aagaagaacg aatcgaacaa cttcctgatt gtttagaaaa
    10921 agagcatttg tttcagataa gcgaaatgtt gagaaacgaa cagtttgatc caaatcaatt
    10981 tttagttgtt gagtacccag ctactggtgt gtattgttgt aatcatgtga agggtgaaaa
    11041 atattttatt attcaagaat acgaaggtaa gttagcgcct tattatacta cttgggaaat
    11101 aaatgaagag ggaatcaata atttcccatg tgaaagcatt gaagagtcta tttctctcac
    11161 ggaatgttaa ataaatagag acgtaaaaga gtcctgaacg tgattgtcca ggactctttt
    11221 tcaatgaatt gttttccgcc aattcactga gtggtgcgat tcaattttat tatatcaaaa
    11281 gaaagtgaga ttccaaagtg aagggaggaa gaagaaatga ctcagacaac gattggtgtt
    11341 gtggttgaaa aaagcaggga taatctcgtt tttgtgacag aaattcaaac aggtagagct
    11401 tttgtagtca cagacaaagc tgcaaaagcg tatcaaaatg gcaatatttt aacgttaaat
    11461 ctgactacga agatgttcgt tgatgcagta gaaaattatc catttgttta gacaaacgat
    11521 attttacaag gaggaaacgg acatggcaga agaaaaagaa ccactagata atagtcggtt
    11581 gggaaagtcc aaaagaaaat tagtacggtt gcaaaacgaa ttaaataatc aatttcaaaa
    11641 gctatttgat caccaaaaat tgacaaatgg gcaaccaatg aatgacaaac gaaatggtcg
    11701 ttcatggttt aaccagcaag aaaaaataga aaataaaata caaagtttga gagaagagat
    11761 caagcaacaa gaaaaacagg tagaaaaact tgaaagacaa gaagagctaa aggaaatggg
    11821 ctataacaag tacggcggac tggatatgac gatcgataat attccaagaa tcaaagaaga
    11881 aatcgaacgc tttgaaaaag gtgaatcgac tttttcagcc gccacgatcc gaaaatatca
    11941 acggaagttg gaaacattag aacagctgaa agaaaaatca gaaaaaggaa aagaaaatat
    12001 cctccctgaa gtacaagcga tcattgacag cggacgagtg acgcaatgga aaaaaaatcc
    12061 aaccatttat tttttgaaag gctatcgaaa agttgcttta gaactgaatg aaaacggggg
    12121 atttgaggaa tcaaaaaaat accaagccac gtcggaagag gaaaaagcag ctgttcgtga
    12181 attacttgaa gaggctgcac caactgattc tctgaaggag aaagaaaaag atcatttcta
    12241 tcgagtagaa ttcaacgaaa atgatccaga aaaaggattg aaaagttatg gtggggagat
    12301 cgtaacatta gaattgatcg atcaacttaa aacgttggat aaccaatacg ctgcagaagc
    12361 aggttattat aaattctatt ttgaagaagt gaagaacgga gaagtagtag atcagatccg
    12421 aatggatcta ggggatggcg tcaacagtaa tcaccccatc tatttagagt tagcaaaatt
    12481 gtgcgaacaa gtacctgaga aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa
    12541 agaacaaatg cagtcgaaag aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc
    12601 agaagagatc aaacaagaag ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa
    12661 gcatacccat tcttctgaag acgtcaaaga acttttgaac tttatgtctc gttttccaga
    12721 acgatccttt agaaatcaat tattgattga ggaacaattt ccaggtgcaa cggcttgttt
    12781 aggtcgtgcc gcgttgaaaa aagaaggaat ctatatcaaa aaaggggaac aagggaacaa
    12841 gatctttgtc agaaaaacgg tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga
    12901 aacagaagct acaccagaag ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa
    12961 aaaaccttac tacacgattg aaaaagtgtt tgatatcaca caaacacagt taaaacctga
    13021 agactatccg aaaatctttc cgaaccgtgt ctttgatttt caattggata aaaaaggaca
    13081 gtcagaactt caagctggga ttcaagcggt agccaacaat attggaatac aaattcgaga
    13141 tatgacagaa agtgaagtct atcaaagaga attaggacaa gcacgtgggg cgtatgttcg
    13201 taacgaattt actggcgaag aagaaatcgt catgaatact cgaaatactc ctacgcagca
    13261 tttagctaca agtattcatg aattagccca tgcaagaatg cacaaatttt cagaattaga
    13321 taccgcaact aaagaattgc aggcagaaat gacttcttat attgtgtgca aacattttgg
    13381 aatggacact tcggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac
    13441 gttggatgat aaggaagcgg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc
    13501 aaatgaattc attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga
    13561 gatccaacca gcagaagtga agcaggagca taaaaatact gtagaaacgg cagagaataa
    13621 gaatattgtg cccgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga
    13681 agatacgggt cgaacgttac atttgaagga tgcggcgttt attgaagatc cgcagcataa
    13741 tcaagtgatt ttaaaaggaa aaagttatgg aaaagagatt caaacggcgt taccttcaga
    13801 agaatttgcg ctaggaaatc cattccgatt ttcacttgaa acagtgtcag aagtggccaa
    13861 acgaaaaaat gaaatctatt atgaacaaca attgtcacaa aaagaacgcg tataaaacaa
    13921 gtaaaaaaga ctatctgaaa aacggatagt cttttttctt ttgggaggtt tgaaaatgac
    13981 cgaaaaaaaa atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg
    14041 aaacaatgga atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc
    14101 atttgttttt gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga
    14161 cattattgaa ttagctaaac tttttttcat agacaaagag atccaagact ctaagcaaca
    14221 atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac gagtggaaaa
    14281 tcttcatttt gaaacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga
    14341 aaaaggaagg aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc
    14401 agaaaaagaa gggttgattg ccgaactaaa gccaaaatat gaacggcaaa atttcttagt
    14461 tcgagacgat cgtttagatc atgcggtcgc ctttttatgg aaagatccac agacaaaaga
    14521 gacagtaggg gcgagttatc aaggaacgtt cattgattat gaacgttttg gtgagagagg
    14581 cacatataag catatagata aaaattcgac agcgaatcat ggctttaacc taaagatcgg
    14641 tgaccctaag cagttaaaat tttttgagag tagtattgat ttacttagct atgctgcact
    14701 gaatcgtgat cagttaaacg atacgtggtt agtctctatg gaagggttaa aacaccatgt
    14761 gatcagtcat tattttggtg aggcagtatc tgaattgaga aagaaacaag cctttcctca
    14821 atccatcgag atttgtgtgg ataatgaccg tgcaggacat attttctacg aaaaagaaca
    14881 attaatgggc gcagtcgatc catttacgaa tcagaaagta cgttgtgaac gaggaatcgc
    14941 taatgattgg caagtgccaa aagaatacaa aatcatttat gaagaagtcg caaaagaaga
    15001 aaaggtcaca ccagaagcta ttatggccat acacaagaca gaaaacaatc tgcaattaac
    15061 gaaccaactg gtttccgctc acaaagtaaa tgcgtttttt ggccagcagc tttctgttaa
    15121 cgattcaata gaagccatta atttgaaaga tatttgtaga gaagtcgcaa aagaattaaa
    15181 agtttgtgaa cgcgtggatg gtacgtacga ttttgatcgt ttttatcaag aaaaagggga
    15241 tattaatgcg cagattcttt tctcttataa agcagaacaa tactacaaag gctataaaaa
    15301 tcatgaacat gaatttatac ctgaagtaaa aaaggactgg aacgatcaat tgaagcatga
    15361 gatccagcaa caagaaatca gaaagcaaaa acgtgccatg ttgtttcaac aaggcagaca
    15421 acaagaaagg gagtgaaatc gtcatgatga aaaccgttat tttagcagaa aagccgtcac
    15481 aagcgaaagc ttatgccgat agcttttcta aagctacccg aaaagatggg tattttgaga
    15541 tccaagatcg attgtttaca ggagaaactg tgatcacgta tggttttggt catttagtcg
    15601 agttagattc gcccgatatg tacgatgaaa aatggaaaca atgggcatta gaacatttgc
    15661 ctattttccc caatcaatac cactatcatg ttcctaaaga taagaaaaag cagtttagtg
    15721 tcgtcaaacg acagctgcag tcagctgata cgattgtgat cgcgactgat agcgatcgtg
    15781 aaggggagct gattgcctgg tcaatcattc aacaagcagg cgccaatcaa ggaaaaacct
    15841 ttaaaagact ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccaacagt
    15901 tacgagacgc agaagagacc tatccaaaat ttgaagaagt acaagcacgt cagatcgccg
    15961 attggttaat tgggatgaac ggtagcccac tgtatagttt actcttgcag caaaaaggca
    16021 taccaggaag tttttcttta ggtagagtcc aaaccccaac actctatatg atttatcaat
    16081 tgcaggaaaa gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gcccaagtaa
    16141 tcgctcaaaa tggcgcattt gatgcgaagc ttgatccaaa tgaaacccaa gcgacacaag
    16201 aagcatttga agcctattta aaagaaaaag gtgttcaatt aggaaaacag ccaggtacga
    16261 tccttcaagt cgaaacagag aaaaaaagtg cggctagccc acgtcttttt tctttatcga
    16321 gccttcagtc taaaatgaat caactcatga aagctagtgc caaagatact ttggaagcca
    16381 tgcaagggtt gtatgaagga aaatatctaa gctatccgcg aacggatacc ccttatatta
    16441 cggaagggga atatgcgtat ctgttggatc acttagacga atacaagcat tttttaaaag
    16501 ccgaagcgat tccgactcct attcatacac cgaatagtcg gtatgtgaat aataagaaag
    16561 tccaggaaca ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac
    16621 aactaagtcc actgcagcag gctatttatg aacaagtgtt aaaaacaacg gtggccatgt
    16681 ttgcggaaaa gtatacctat gaagaaacaa cgatcttgac gcaagttcag cagcttcagt
    16741 taaaggcaat cgaaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga
    16801 gcgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg
    16861 tgacggtgga tctacaagtt cttgaaaaag aaaccaagcc accacagcct tataccgaag
    16921 gtacgttgat cacggcgatg aaaacagctg ggaaaaccgt tgatagtgaa gaagcgcaat
    16981 ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa
    17041 ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag
    17101 ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgagtgcc gaaatgaccg
    17161 ccaaatggga aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc
    17221 tagccaatat ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat
    17281 ccactgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg
    17341 cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg
    17401 tggcagcatg tacgtctgaa gcctgtgatt tcaaactatg gacaacgatt gccaaaaaga
    17461 aacttacagc gacacagctg aaagagatca ttcaaaatag ccgaacaagc caaccagtga
    17521 aagggttaaa aggtcagaag ggttcgtttg aagcaacgat tgttttaaaa gaagacttca
    17581 ctacaggctt tgaattttct gaaaagaaaa aaacgaactt caagaaaaga acacgacgca
    17641 ctacaaaata atggagggtt aatcttatgc gtaatacaac acacaaacaa caagaaatcg
    17701 aaaatcaaaa agccaaaatt cgttcagaac aggaaaaaat gcgtgccagc aaacgaagac
    17761 tgcagcaatt gaagaaccag cagcggattc aaaaagaaat cgaattagat cgataaattc
    17821 ctttcttgtt aatataaaac taacatgtta tagttgtgtt agttttatat taactttagg
    17881 ggaagagtaa tgaaagacaa aatccaatgt atttttgata aacataatgg caacctatcc
    17941 ttgaaggatg ctagaagtga aggattatcg cctatgacgt tagatcgatt agccgaaaag
    18001 gataaaatag aaaaggtagc accaggtttc tataccttaa aggatgcgtt cgtggatgaa
    18061 ttgtatctcg ctcagagtat ctattccaga gggatttttt ctcatgagac tgcgctcgat
    18121 ctttttcaag taggaacata tatccctaaa aagatcaata tgatgtttcc taaaggatac
    18181 aatgtatcaa aggagaaatt agatcagtat gccattcaac cgcattacac taataaagaa
    18241 aattttgacc tgggagtcac agaaacagaa agcttttacg gcaataaaat tttagtctat
    18301 gatttagagc gaacgttatg tgacatgtgg aatccaagat acaaagcaag cgtagaggta
    18361 aaacaagaag cattaaaaga atatatgaca agtaccagta gaaatacaaa taagctaaga
    18421 agatacatgg atattttaaa atcaccaaaa gaaatgactt tgtatatgtt gccactttat
    18481 tgagaggagt aaagatgaat gacaccgaca caatttaaag agaaaacaag aaatttcgca
    18541 gcagagaaac atttaaaccc acaaattgtc caaagatatt atatgatgga aaaattatta
    18601 gaatgtattg ttgaatcgcc ttatcatgat gattttgtat taaaaggggg atttctgatt
    18661 ggatcaaaat acgggttaga aaaccgttca accgttgata ttgatacgac ttatcgaaat
    18721 tcgaaactaa caaaagaaaa gttagaaact gttttttctg aattaacagc taatcctaca
    18781 aaagatggaa ttcgttttga attaaaggga ttaacagaga cgagggaagc cgattattat
    18841 ccaggttttc aagcaaagtt tattgcttat cttgaaaatg cacgtgtacc ttttaaatta
    18901 gatattactt caggtgattc tattattcca gcagcattga tgtatgaaca caaattaatg
    18961 tttgaagata aaacgattca tattccagct tatcctacgg aacaaattct agctgaaaaa
    19021 ttacatgcca cgtttagttt tggaaaagat aattcgcgca tgaaagattt ttatgacctg
    19081 tatatgattc cgaaattaga acgtatcgat gagaaaaatt tgtataagag tgttcaagga
    19141 acattcaagg aaagaaataa tcaatcggtg tttaagcatt tgtatgatca ggaaatgcct
    19201 caattggtga aagatgaggg cttgagagaa aagtggaaaa gataccaaca agaaaactat
    19261 tttgccaaag acatttcttt cgaatctaca ctaacttccg ttgattccct gatgcaatca
    19321 attgtagagc aagagaaggc agaacgaaaa caaaatttta agagatcaca acaaatgaat
    19381 caagaaatgg agagataaag atgatcaata atgtaacttt agtagggcga ttaactaaag
    19441 atccagattt acgttatacc gcaagtggca cagcagtggc gactttttcc ctagccgtta
    19501 atcgtaattt cacgaatcaa aacggcaatc gagaagctga ttttatcaat tgcgtgattt
    19561 ggcgcaaacc agcagaaacg atggcgacat tagctaaaaa aggcagcttg atcggcgttg
    19621 tcggacgaat tcaaacacgg acttatgaca accagcaagg acaacgtgtg tatgtgacag
    19681 aagttgtggc cgataacttc caactattag aaagtaaagc agcgacagaa agccgtatgc
    19741 acgctgatca atcgtccagc agtccaagcc ctaccacctt tgaacaacgg gatacagcga
    19801 cgcctaacaa caatggattg aacgcttcac agaatccatt tggtggacaa tcaatcgata
    19861 ttagcgatga tgatctaccg ttctaagtag cattttctat tggttgcatt tgctacggtt
    19921 tatgttataa tttagacaac aaaaagaatt ttcaatctat aaccaaaaac cactagctgg
    19981 cgggctagtg gctttctttt acgtttctat tataagaatt aacgggtagg cttactgtct
    20041 ttcttggcaa atttacaatc aagccatttg cagatatagt gcgcggctac ttctgccatg
    20101 attgcaaaaa caaaatcaaa aagaattttc aaacctataa cctcctcccc ttgccagatt
    20161 aggccggggt agcagacagc aagactatta tagcataatt gaaatatcca aatgattgga
    20221 tttttgctat aattttagcg aggtgattgt gatgaacaaa tatgttggtt tgcttgataa
    20281 aatccgagta ataaaaacgc aaccgttatt ggttcgcttt actttacaaa cgattcatga
    20341 atcgattaat tgtgtagttg cagatattga aattatcgat aaattgctaa ttatggatga
    20401 tggaaaatac aatattgccg tcacaggtca cttcaataaa cgaaatcagc tagttattgc
    20461 tagcatgtat gttagaaatc ccgatcattt tacgcgatcg atgggcatat aaataaaaga
    20521 agagttgtag tcctaaatga ggattgcagc tctttttgtt ttacatagaa acaggaaaag
    20581 acctttttag gaagacacaa gtgccttctc aattgaaaaa cctttctctc actccaaccg
    20641 ccaccgccgt tcgagaagaa aggaagtgaa cgctgcacgt tcactaagat atggtgtctt
    20701 gcaaagcaag acaaaataga caaaacttcc tctcaccaaa aggttcgttc ctgttcgtta
    20761 tctctttatt gatttgccag accggcaaca agttgccttt tatcaccaaa aagcgacaag
    20821 gacaagggta aatggcacgc tgcgcgccct tgtccttgtc gcttttcgtt gatttattct
    20881 ggccaacaaa tcaataaaga aagaactaac gaaaggaagt aaaacctttc ctaagaggag
    20941 gcagaatcaa acatgcgaaa gaaatttgat tattggggtg ttcccttcag cgaattattc
    21001 ccaaactatc atgcaccaca tactgttgaa tgcgattgtg gagaaagagc gaaatgtatc
    21061 aaaagctatc gtttgtatca atgtccaaca tgcgggaaaa aatatacctt gtcttatggc
    21121 gattacattt tgattgatga gaaaggaaag aaacgctgat gaaaacacaa cacaaattat
    21181 tgactgtaaa agaagtcgtt caaattttgg tggacaaaga agtcatttct gaaaaagtag
    21241 cagaagcttt acttattccg cggtactgga cgatagaaac ccctttagta gaggatgcac
    21301 cgactatgct atttgaagac ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa
    21361 acatggaaac ttatgaaatc gaatattggt taaccaaatt agagaactaa aaaggagaaa
    21421 aaatatggct caaaattact atgacgaatt tgtcaaatta cctttggata aaatggcaca
    21481 aaaaatggag gatatgacgt tcctatacca tgaaacacgt gtacccaaaa aacattacaa
    21541 ggaaaaattg tcagtcgcag tggaagaaat gattgaatca ggtgtcgaaa tgaatctcat
    21601 tgcgacgtac tatcgaacac tggaagaact gaaaaaacaa aatggaaaat ggtttttcca
    21661 agccttacta tgcttggaag taggtgtcaa accaagcact atcaaacctt ccgaatatca
    21721 agccttagaa ctgacttatg caaaattcgc acagattaag aaagctaaag caatggctag
    21781 cgactggcta gatacttttg aaagtatcga aaaaaatggt gcattttata cgattgaatc
    21841 ggaggagaaa gataatgaat aaggtggttt atcagctaac aacagaagat gtttcgcaat
    21901 ttttaacaag caaagaacac ttgaatgaag taatcagacg gattcaaaaa gactttagtt
    21961 gtatggaaga aatgaagtac tttattcaaa gagtggaaga acagttgtat atggaaagcg
    22021 aaacaagtca aacgaaggtc acagtcagcc ataatcattt agacgtgtat aaacgtcgtc
    22081 ctgaaacggt aaaaaaagga acagagcttc tacgactaga aacagaaaaa tacatcatga
    22141 atgtgtacct agaaaggagt gagctggtcg aaaactttgt cagctacacg atttgtagga
    22201 aacactatta ctcagaatca gaatattatt caattttcga gagcatggaa ttaatggaag
    22261 aagaaatgga agagctaaac agcaaaaatg cgacggtaga cgattattac cttgtattta
    22321 ctaggttttt gacgtgtggg acggaatcta tcgtgttaat gggaatggat tatgaactcg
    22381 ctgtacccga agcatttttg agggaatatg tagaagaagt agataaccca ggcgacgact
    22441 ggtacacgac tttaactgat tttttagaca cctacactta cgacgataca gaagaaatcc
    22501 aaaatgaact tgaccgtaaa cgaatgccat atcaacgatt tgaaaggaaa aaaggtaaat
    22561 aaccatttat accgaaaagt gaggatgtgt gaaataagaa caaaaaacct tgaaattaag
    22621 ggaatggttc ttatttcgca ccaatcgctc caaccgcccc cgccgcttag gtggtgcgaa
    22681 ataagcgggc aaagcccgat aacctaagag aaagaaggag atccatatga ataaaatcat
    22741 tcctctattg ttcgtactgt ttgttggcga gatacttatg cgtcatgctg cagctgtaca
    22801 tttaaacttc gtagtattct taggaatact ggtattttgt ttgatctttt ggcgatttgt
    22861 attagccctt gtgagtctac tattcaagat gattgcagcc atatttttca tagggttatt
    22921 tcgatgaaag gagagagtac taatgccaat tgcaatagga aataaacggt tacccgtgac
    22981 attagatgaa aagagacaaa aagaattgca gcaactaaag cagaagtacg gaaaaagtga
    23041 atctaagatt atgtgtattg cgttagatct attgatcgcg caagaaaaag caggatttga
    23101 tataccagca ctaaaaaagt gacgtcactt ttcatttgaa gaaacaaaag tgatagcact
    23161 taattgttcc tactatttag aatattttct atgtcctttt tattccattt tgtatcaata
    23221 actatgaaat ctttaaagta tccagaagag attattttta gatcaatttt tttcttacct
    23281 gattctaaag attttgtttt cttaaacata ttatgatttc ctttcgacta ttttcatata
    23341 aatgttaaca tagtttatgg attcatggaa tgttaatcta atttaataga aaaggataat
    23401 taagtatata cgttttttct tatgtatgtt atactatgta tatacagaaa gaggtgtcta
    23461 ctatggaaat gttacctata aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt
    23521 atattatgga atatcttgga attcatacag aagacaaagt gaagataata caagaagaag
    23581 taaacggtca gaagcggcta attatcgaag cggctaatat agaaaatgaa ttaacaattg
    23641 aacagttatt tgaaaattac actgatgaaa gaaatcatgt gacgattcaa aatttaggtg
    23701 aagcagtagg taatgaaaaa tggtgagaaa acctagacaa ggcgatattc tattattgaa
    23761 tacagcacct cgatctggac acgaacagac aggtaaaaga ccttatattg ttttaagtca
    23821 tgatattatt gcggattata gtaacgttgt tacagttgct ccaatttcta gtacaactag
    23881 aaactatccg ctatatgtaa atataaactc agaatatgaa atgaagacaa caggaaaagt
    23941 attattagat caactaacga caattgatta tgaagccaga gagtgtattt ttttggaaaa
    24001 agcgcatgat agtttagttg aggagttatt gatgaaagtt agaacagttt ttcaaaaagt
    24061 aagcaaataa aatagaaaca gcagcatgat caactattcg tgattgacca tgctgtcttt
    24121 ttttattcta tatacggttt gtctcgtaac attgtagtct tttgcaatct tagcgatcgc
    24181 aacacctttt tttagatcct caacgatgtt tttataaaca agacgacgtt ggggatcttt
    24241 tgcattggca ctgtatagct taggacgtcc tttataaaca ccgttcgctt ttgcaatctt
    24301 aatgccttgt gcctgacgtc gtttagattc ggtacgttct tgttcagcga tcatggccaa
    24361 aatttgaatg atcaaatctt taatgaattt atccaggaga ggattgccaa ttgcttcggc
    24421 catgatcggt agacttgtga tgatcagctg cacatttttc ttcttcaagt aattgactga
    24481 agcgatgatc tcgtcgtaat ttcttccaag tcgatcaata gcttccacaa taaaaatatc
    24541 ttgatctcgg acgaaatcca gtgcttcttg aaaaacaagt cgatgagtaa tcgttgcgcc
    24601 agattgtttc tcaatgaaaa ttctctctgc accgaatttt ttcatttctt caatctgccg
    24661 ttgttcattt tgatcgatgg atgagacacg tatgtatgca actctcaaaa tttttaccta
    24721 ctttctgcaa ctatttaaaa aacaccctaa tcatacacga gaaaaccaga tttttcaatg
    24781 tcgcggtcgt gtctagttag ggtatacctt atatagacaa aaactaatga tggaggaaat
    24841 agtatgtgtc acttttcttt ttaaatggca atattttttg taaataaatg tcaatcaaga
    24901 aaatttaaac gattatgaca ttaaaaatat attttatgaa ttttctagta tataacttga
    24961 tttatctgtt aagatttatt aagaaaacgt tttcttattt ttgatttcga ggtgatagac
    25021 aatgaaaaat aagtttattt tatctttagg tattttaact attggaatta gtgtaactgg
    25081 aactacaatt cctacatttg ctgatagtac aaatgaacca actactgtag tagataataa
    25141 ccaagaatta actcctttag ataggaacgg aatagccatt ggtaacccaa ctaacgactt
    25201 aactgttact gaaaattatt taatagtttc tccattactt gctcgagcag cttctaaaac
    25261 gatacgagtg acaggaacga tgatttttga taaagttggt tggccttcta gacctcctca
    25321 atcacggaat tggaaagaaa aaagaaatgg ttattggtac tatggaaaat tgtatttaca
    25381 aagttataaa tcagctggaa acaattggat tgcgacttat tcaggaacct taactagaaa
    25441 gtagggattt tttatgccag aatttgtgaa cacaccaaat ttttatcaag atagcgatat
    25501 tctaagtaaa attaatttac cgaaagaaga atcagcttta caagttcagc gaactttaca
    25561 ggagacacaa aaaattcaaa aatcaatttc taatctagaa gattctgaag aaaaaagaaa
    25621 aaaaagagac caaaaaacat atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta
    25681 gtcgacattc tttttttcta attgaaagtg ttcataaaat cacccatttc tggacacttt
    25741 ctttttttgc tttatttttc aaaaaaacaa ttcatttcat gttcataaac ccataacaat
    25801 atctatgttc agttaaacta tatggataaa gttatgttac aataaaatta atgaaggagt
    25861 gatttttttg aaaataggat acgcccgggt atctactggt ttacaaaatt tggatttaca
    25921 agaagacagc ctaaataaat ttggttgtga aaaaattttt actgatcata tgagtggggc
    25981 aaaaagtaat cgaccaggat tagaaatggc cattgatttt gttcgctctg gcgatacact
    26041 tgtcgtttgg cgcttagatc gtttaggtag aaatatggaa gatttgattt caatcgtcaa
    26101 tcgactgaat gagcgtggtg tgagttttca tagtattcaa gaaaatatta cgatggacaa
    26161 atcaagttct acaggacagc tcatgtttca tttgtttgcg gcatttgcag aatttgagcg
    26221 taatttgatc ctggaacgat ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt
    26281 tggtggtcgt cctgaaaaat tgtctgaaca agatttggaa ttactgaaga ctttagtgga
    26341 tagcggtaca cctattaaga ctattgcgga acggtggaac gtctcaagaa ccaccattta
    26401 tcgttatttg gcaaaaatgg aggagaaaaa tggaaagaca aattctcatt agtttttgtc
    26461 atttaatgct gaaaattttt gccatttatc tagaaaagtg acaatagtat gagaattttt
    26521 tgtaagtgtc tactgataac ttattctcaa atgttgtaag gattttattc atttgttaat
    26581 atta
//
LOCUS       DAYYBH010000033        26153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000033 DAYYBH010000000
VERSION     DAYYBH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.033"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACHAUG_001549"
     CDS             133..3660
                     /locus_tag="ACHAUG_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8480642.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACHAUG_001550"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACHAUG_001550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8480643.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACHAUG_001551"
     CDS             7448..7789
                     /locus_tag="ACHAUG_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8480644.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACHAUG_001552"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACHAUG_001552"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8480645.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACHAUG_001553"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACHAUG_001553"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8480646.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHAUG_001554"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHAUG_001554"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8480647.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACHAUG_001555"
     CDS             13000..13737
                     /locus_tag="ACHAUG_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8480648.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACHAUG_001556"
     CDS             13751..14566
                     /locus_tag="ACHAUG_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8480649.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACHAUG_001557"
     CDS             14579..15226
                     /locus_tag="ACHAUG_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8480650.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACHAUG_001558"
     CDS             15242..15898
                     /locus_tag="ACHAUG_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8480651.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACHAUG_001559"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACHAUG_001559"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8480652.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACHAUG_001560"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACHAUG_001560"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8480653.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACHAUG_001561"
     CDS             18240..18758
                     /locus_tag="ACHAUG_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8480654.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHAUG_001562"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHAUG_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8480655.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACHAUG_001563"
     CDS             complement(19645..20565)
                     /locus_tag="ACHAUG_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8480656.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACHAUG_001564"
     CDS             20691..21743
                     /locus_tag="ACHAUG_001564"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8480657.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACHAUG_001565"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACHAUG_001565"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8480658.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACHAUG_001566"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACHAUG_001566"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8480659.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACHAUG_001567"
     CDS             complement(23383..24144)
                     /locus_tag="ACHAUG_001567"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8480660.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACHAUG_001568"
     CDS             24333..25427
                     /locus_tag="ACHAUG_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8480661.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>26153)
                     /locus_tag="ACHAUG_001569"
     CDS             complement(25513..>26153)
                     /locus_tag="ACHAUG_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8480662.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8644 a         4531 c         5508 g         7470 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat tctcgtttaa tgggcgttga gaaggtgttc cagggtccgc
    25741 aaaatatata tcaatatcat gttggttact aatggctttc cagttagaaa attcttttcc
    25801 acagtcaaac gtaatagatt taaagaagtt tttagggaag cgagaaaacc attgattaag
    25861 ggcagtttca atatctaatg ccttacggcc gttgggttta atcgtgataa tgactttaga
    25921 taatctttca actaaagtaa tgacggcact tttatgatga atgccaacga tcgtatcacc
    25981 ttcaaggtga ccaaactcag aattgaaatc aggataatta tcaggtcgat catggattga
    26041 gcgctgatac tgttgttttc cccgtttttc ctgatggcca ttgggttttc ttttaccttt
    26101 catcggtagt gtgtcaatat caaatattcc tttagaaaat aaacgataaa gtg
//
LOCUS       DAYYBH010000034        26119 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000034 DAYYBH010000000
VERSION     DAYYBH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.034"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHAUG_001570"
     CDS             complement(<1..1835)
                     /locus_tag="ACHAUG_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8480663.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHAUG_001571"
     CDS             complement(1832..2536)
                     /locus_tag="ACHAUG_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8480664.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHAUG_001572"
     CDS             complement(2548..4857)
                     /locus_tag="ACHAUG_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8480665.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHAUG_001573"
     CDS             complement(5052..5516)
                     /locus_tag="ACHAUG_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480666.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHAUG_001574"
     CDS             complement(5516..6142)
                     /locus_tag="ACHAUG_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8480667.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHAUG_001575"
     CDS             complement(6149..6514)
                     /locus_tag="ACHAUG_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8480668.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHAUG_001576"
     CDS             complement(6504..6842)
                     /locus_tag="ACHAUG_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480669.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHAUG_001577"
     CDS             complement(6832..7158)
                     /locus_tag="ACHAUG_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8480670.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACHAUG_001578"
     CDS             complement(7136..7417)
                     /locus_tag="ACHAUG_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8480671.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACHAUG_001579"
     CDS             complement(7419..8783)
                     /locus_tag="ACHAUG_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8480672.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHAUG_001580"
     CDS             complement(8796..9365)
                     /locus_tag="ACHAUG_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8480673.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHAUG_001581"
     CDS             complement(9337..10524)
                     /locus_tag="ACHAUG_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8480674.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHAUG_001582"
     CDS             complement(10588..12315)
                     /locus_tag="ACHAUG_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8480675.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHAUG_001583"
     CDS             complement(12312..12764)
                     /locus_tag="ACHAUG_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8480676.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHAUG_001584"
     CDS             complement(12876..13256)
                     /locus_tag="ACHAUG_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8480677.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHAUG_001585"
     CDS             complement(13253..13639)
                     /locus_tag="ACHAUG_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8480678.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHAUG_001586"
     CDS             complement(13641..13919)
                     /locus_tag="ACHAUG_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480679.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACHAUG_001587"
     CDS             13967..14695
                     /locus_tag="ACHAUG_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8480680.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACHAUG_001588"
     CDS             complement(14841..15317)
                     /locus_tag="ACHAUG_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8480681.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACHAUG_001589"
     CDS             complement(15277..15633)
                     /locus_tag="ACHAUG_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480682.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACHAUG_001590"
     CDS             complement(15611..15796)
                     /locus_tag="ACHAUG_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480683.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACHAUG_001591"
     CDS             complement(15797..16069)
                     /locus_tag="ACHAUG_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8480684.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACHAUG_001592"
     CDS             complement(16066..16266)
                     /locus_tag="ACHAUG_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480685.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACHAUG_001593"
     CDS             16394..16606
                     /locus_tag="ACHAUG_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480686.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACHAUG_001594"
     CDS             complement(16639..16833)
                     /locus_tag="ACHAUG_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480687.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="ACHAUG_001595"
     CDS             complement(16846..17028)
                     /locus_tag="ACHAUG_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480688.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="ACHAUG_001596"
     CDS             complement(17025..17165)
                     /locus_tag="ACHAUG_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480689.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="ACHAUG_001597"
     CDS             complement(17655..18020)
                     /locus_tag="ACHAUG_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480690.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="ACHAUG_001598"
     CDS             complement(18060..18362)
                     /locus_tag="ACHAUG_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8480691.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="ACHAUG_001599"
     CDS             complement(18362..18661)
                     /locus_tag="ACHAUG_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480692.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="ACHAUG_001600"
     CDS             complement(18654..18818)
                     /locus_tag="ACHAUG_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480693.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="ACHAUG_001601"
     CDS             complement(18824..19093)
                     /locus_tag="ACHAUG_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480694.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="ACHAUG_001602"
     CDS             complement(19105..19854)
                     /locus_tag="ACHAUG_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480695.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="ACHAUG_001603"
     CDS             complement(19851..20543)
                     /locus_tag="ACHAUG_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8480696.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="ACHAUG_001604"
     CDS             complement(20549..21220)
                     /locus_tag="ACHAUG_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8480697.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="ACHAUG_001605"
     CDS             complement(21213..21554)
                     /locus_tag="ACHAUG_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480698.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..22430)
                     /locus_tag="ACHAUG_001606"
     CDS             complement(21732..22430)
                     /locus_tag="ACHAUG_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8480699.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22517..22987
                     /locus_tag="ACHAUG_001607"
     CDS             22517..22987
                     /locus_tag="ACHAUG_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480700.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23030..23209)
                     /locus_tag="ACHAUG_001608"
     CDS             complement(23030..23209)
                     /locus_tag="ACHAUG_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480701.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23196..23534)
                     /locus_tag="ACHAUG_001609"
     CDS             complement(23196..23534)
                     /locus_tag="ACHAUG_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8480702.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23550..23753)
                     /locus_tag="ACHAUG_001610"
     CDS             complement(23550..23753)
                     /locus_tag="ACHAUG_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480703.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23768..24544)
                     /locus_tag="ACHAUG_001611"
     CDS             complement(23768..24544)
                     /locus_tag="ACHAUG_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8480704.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24843..25163
                     /locus_tag="ACHAUG_001612"
     CDS             24843..25163
                     /locus_tag="ACHAUG_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8480705.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25181..25612
                     /locus_tag="ACHAUG_001613"
     CDS             25181..25612
                     /locus_tag="ACHAUG_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8480706.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            25742..>26119
                     /locus_tag="ACHAUG_001614"
     CDS             25742..>26119
                     /locus_tag="ACHAUG_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8480707.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEI"
BASE COUNT         7402 a         5365 c         4051 g         9301 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagcttc tttatttttc aaaattacta attcttgcat tatttcttct
    22441 ctcctttttg gtataattta gttaaaaact ggtggtgttt attttgaatt tttttcactt
    22501 aacaattttt caatggatgt caataataag ttttatattg agtacgtttt taacagggat
    22561 aaaaataaaa ggaatgcgcc ctcaactaga agttgaactt aatgcttctt acttcgctgc
    22621 agatatgatt tatacaaaag taatcatttc taactattca acagaaccag ctatgttagt
    22681 taatttagaa ctttcatccg ctaacttaaa ccgcagatgg tcagctactc catttaagaa
    22741 attaattgct aaaggaggat cagtcgatga taatagaata tactctgaag ctgtaccgct
    22801 aaatattcct cctaaaagtg ctatatcttg ttaccttgca tttgaagtag ggaaaattaa
    22861 ttttaataaa ctccttaatc accaaacaaa aatgatattt actctaaata gaacccaaat
    22921 ccacaaaatt gttgatatca aaaatacaaa cttcccaata gagaaattag tgaaagaatt
    22981 aaactgagta atagtaatat acattctagt ttctcttttc tattcatttt tagtcagccc
    23041 cctcggttgg ctttttcgct ctgtactcag cttcagcttc atcaagcccc ataaaaatcc
    23101 aaaccatgta aacaatcgtt cctatcaacg cttgcctgct tccccaaagt cctaaagcgt
    23161 agatgattag cggtgcgctg aacactagcg ctctattaaa ttttcccatc cgcttgcctc
    23221 cttaagattt ccgaaaaatt tgtttctaaa aattcaagtg ttttacttct taaaaataga
    23281 tatgtgtctc ttccttcaac tggataatag acaaatccat ttttgttttt ctcgatatcg
    23341 ataatatttc tatatcttgg gttctttaaa actctagaag taaaccaatc atattttctg
    23401 ttaatccgtt ctagcacttc tggcaacgtc atccatctac cagtatcatc agcttttttt
    23461 aactcctcat aatccacttg ggagataatt acatagcctt ctggaattgg gatttttgct
    23521 tctagatatt gcattagctg ttcctccctt tacaattcgt acatagtgat aatcgaatct
    23581 ataattctgt ttgcttctgc agaagtcttt ttaccgttta aaattaaaga taagtagctt
    23641 ttgctaattc caaatctttc agcaagcatg gtgtaagtta agaactttga actttcgaca
    23701 tattctttga ttttttctct atctcgttga gtgatttctg caatgtcagt catactaaaa
    23761 ctcctttcta accaatttcc tctaaatcca tttgagggta atatccttct ttttttagta
    23821 attcgtagat aaatagacgc cctttctgtg tccatttggt attcattaca attttagtgc
    23881 caccatcggc tttcgggatc tcagttgtat gagattttgt gtatccttgg ttcatgtgtt
    23941 ttttgcacaa taaccattgg ttaccgactt ttttctgaat acctagttta tgaagtaatt
    24001 tattcatctg ttgtggagac atcccataat ctgctgcaat ctgactaatt gttactgaat
    24061 ctgtagaaga taatatgcta tctaaatagg agattttagg ttcgtactct gcaatctttt
    24121 gttctgcgat taatcttcca gttctttctt cttttagttg agttgctaat tgaatgattg
    24181 tatctggatt aagcaaagct tcttctactt tttctggagt tagataacct ccatgttttc
    24241 taattgctgg caacacttca cttgttaccc aacgtttaaa ttttttggca gagggaagtt
    24301 ttgattttaa gattaaactg tacaaaccag attcgttgat gattgtcatc tctcttgatt
    24361 gacctgaggt cgtgattcgc gaccccatct tatcttccaa atctacatgc cgcgacaaag
    24421 catctttagt gtttgaataa cccaaaacac tcgcaacgtc tttgcctaca aaatatggtt
    24481 catcatttac taaaattgtc cgaacttcgt tttgttcgaa attaaaaatt tgcggtgtgt
    24541 tcatatttct cattcctttc ttttatattc gtaaacaaat ttaacaactt ttttctaaat
    24601 tcaattgaca gtatctgagt aatactctat actaagagca taattaaata agacataaaa
    24661 acattgattt taaagctttc ttggcggtct gcatttacat atcaatagtg ttttttgttg
    24721 tctttttagt tgttaaatct gtttacaaga tatagtatag ctcattactc agaattagtc
    24781 aaattatttc tgcgtatttt tctaaacttt ttttgtaaac atttagaaag gttgttttat
    24841 cgatgaatac ctatgaaata ataaaagact tggcgaagag gaaaaagata tctatacggc
    24901 agttagaaat taattttgga tattctaacg gttatctagc aaagtggaaa aataatactc
    24961 ctaattcaga tgaattaagt cggattgctg actatttcga tgtctctgta gactaccttc
    25021 taggtagaga agaaagagaa acccctaaac atgtggattt atcagaagac gatactgttt
    25081 tttcttttga tggaaaagaa atatctaagg agacaatgcg taaagcgatt gcaattgcta
    25141 aagctttaga ggaaaatgaa tagttggagt gatggtttgt atgtatttga aattgaaaga
    25201 aatgttgagc gaatacaatt tgaaagtagt ttatatggag atgaaagagc ctggtttcta
    25261 ctatccaaaa cccagaatca tttttttgaa tgaaaatcta tacggtgaaa ctgcagaagc
    25321 ctttcattta tctcatgaac ttgcacattt cagcgcctct cattttgaat tttcagtttt
    25381 gtacgatact tctacaactt ttcactcaaa atttgaaaca gaagcagata aaatagctat
    25441 tctaatttta ttaaatatct atattgagaa cgaactgact gatgaatctc agtttaatct
    25501 cgaaaaattt atggagtatt attctattca gaataagctc agatacactt gttatgctgt
    25561 ctgccaatgc tattttaaga aaaaatattc ttacgcaagg caatatgtat gaatacatca
    25621 agaattatta aaaaaaccga tttcagcaaa acattaaaaa agcccgtgct gcaacacggg
    25681 ctctttcctc atatatgagc ttctacaaaa aaaatcatat cacagaaatg aggaagaaaa
    25741 aatggaaaaa gagattttaa taaaatctcc aggaaaaacc ttgattaaag taactgatga
    25801 ttctatttca attattagaa aaggatttat aaatttagtt aatcaaggta ttaaaggaga
    25861 aaaaactatc ccctttaaaa atatatcagc cgtacaatta aaaaaaccag gtatgagtaa
    25921 tggttatata caatttacct tgctaggcgg aaacgaaagt cgcggaggta tattagcggc
    25981 cacgaaagat gaaaacaccg ttatgtttac aaaaaaatat tggaatgaaa tggaaaattt
    26041 aaaaaaatat atagagaagc aacaatctat attagataat aattccaaag aaacacaaat
    26101 ttcaagtgca gatgaaatt
//
LOCUS       DAYYBH010000035        26068 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000035 DAYYBH010000000
VERSION     DAYYBH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.035"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHAUG_001615"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHAUG_001615"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8480708.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(1926..3221)
                     /gene="purB"
                     /locus_tag="ACHAUG_001616"
     CDS             complement(1926..3221)
                     /gene="purB"
                     /locus_tag="ACHAUG_001616"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8480709.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3234..4358)
                     /gene="purK"
                     /locus_tag="ACHAUG_001617"
     CDS             complement(3234..4358)
                     /gene="purK"
                     /locus_tag="ACHAUG_001617"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8480710.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(4351..4839)
                     /gene="purE"
                     /locus_tag="ACHAUG_001618"
     CDS             complement(4351..4839)
                     /gene="purE"
                     /locus_tag="ACHAUG_001618"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8480711.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(5255..6562)
                     /locus_tag="ACHAUG_001619"
     CDS             complement(5255..6562)
                     /locus_tag="ACHAUG_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8480712.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(6565..7146)
                     /locus_tag="ACHAUG_001620"
     CDS             complement(6565..7146)
                     /locus_tag="ACHAUG_001620"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8480713.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(7262..7359)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(7718..9010)
                     /locus_tag="ACHAUG_001621"
     CDS             complement(7718..9010)
                     /locus_tag="ACHAUG_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8480714.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(9007..9630)
                     /locus_tag="ACHAUG_001622"
     CDS             complement(9007..9630)
                     /locus_tag="ACHAUG_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480715.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(9633..10469)
                     /locus_tag="ACHAUG_001623"
     CDS             complement(9633..10469)
                     /locus_tag="ACHAUG_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480716.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(10486..11373)
                     /locus_tag="ACHAUG_001624"
     CDS             complement(10486..11373)
                     /locus_tag="ACHAUG_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8480717.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(11389..12672)
                     /locus_tag="ACHAUG_001625"
     CDS             complement(11389..12672)
                     /locus_tag="ACHAUG_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8480718.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(13355..14038)
                     /locus_tag="ACHAUG_001626"
     CDS             complement(13355..14038)
                     /locus_tag="ACHAUG_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8480719.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(14056..14673)
                     /locus_tag="ACHAUG_001627"
     CDS             complement(14056..14673)
                     /locus_tag="ACHAUG_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8480720.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(14683..15507)
                     /locus_tag="ACHAUG_001628"
     CDS             complement(14683..15507)
                     /locus_tag="ACHAUG_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8480721.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(15500..16060)
                     /locus_tag="ACHAUG_001629"
     CDS             complement(15500..16060)
                     /locus_tag="ACHAUG_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480722.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            16234..17190
                     /locus_tag="ACHAUG_001630"
     CDS             16234..17190
                     /locus_tag="ACHAUG_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8480723.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(17495..18451)
                     /locus_tag="ACHAUG_001631"
     CDS             complement(17495..18451)
                     /locus_tag="ACHAUG_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8480724.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            18555..19157
                     /locus_tag="ACHAUG_001632"
     CDS             18555..19157
                     /locus_tag="ACHAUG_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8480725.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(19245..20744)
                     /locus_tag="ACHAUG_001633"
     CDS             complement(19245..20744)
                     /locus_tag="ACHAUG_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8480726.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(20760..21284)
                     /locus_tag="ACHAUG_001634"
     CDS             complement(20760..21284)
                     /locus_tag="ACHAUG_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8480727.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            21582..22331
                     /locus_tag="ACHAUG_001635"
     CDS             21582..22331
                     /locus_tag="ACHAUG_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8480728.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            22322..23107
                     /locus_tag="ACHAUG_001636"
     CDS             22322..23107
                     /locus_tag="ACHAUG_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8480729.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            23076..23792
                     /locus_tag="ACHAUG_001637"
     CDS             23076..23792
                     /locus_tag="ACHAUG_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8480730.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(23894..24913)
                     /locus_tag="ACHAUG_001638"
     CDS             complement(23894..24913)
                     /locus_tag="ACHAUG_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8480731.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(24932..25933)
                     /locus_tag="ACHAUG_001639"
     CDS             complement(24932..25933)
                     /locus_tag="ACHAUG_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8480732.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7819 a         5567 c         4662 g         8020 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagttg
     1861 gctttcccct ctactcgatt atttttgcta ttctatttta attgaatttt gcaactcact
     1921 cttctttatt ccaaacctac tcgtttgaat atcacgtcaa cgtttttcaa atgatgatga
     1981 taatcaaagg catcgtctat ttcttcttta gataataccg atgtgatggt tggatcttct
     2041 tctaacagcg ggcggaactg cacttggttg tcccaagcgt acgcagtttt tggctgaaca
     2101 agatcataag cttcttctct tgtcattccc ttatcgatca atttcagcat aacgcgctgg
     2161 ctataaatca aaccaaatgt tgcagacata ttccgcttca tgttttcagg aaagaccgtt
     2221 agatttttaa cgatattgcc aaaacgattt aacatgtaat tcaataaaat cgtggtatca
     2281 ggcaagatga ttcgttctgc tgaagaatga gaaatatctc tttcatgcca taatgtgaca
     2341 ttttcatagg cagtgaccat gtgtccgcgc accactctgg ctagaccagc catattttct
     2401 gaaccgatcg gattcctttt gtgcggcata gcagaagaac ctttttgacc tttcgcaaaa
     2461 aattcttcta cttcacgtgt ttcggatttt tgcaatccgc gtatctctgt tgcaaaacgt
     2521 tcaatacttg tagcaataag cgccatcgct gagaaatatt cagcatgtag atcccgaggg
     2581 agaacttgag tagagatttc ttgtggacgg atccccaatt gtttacatac atactcttca
     2641 ataaatggcg ggatatttgc gaacgttccc actgctcctg agatttttcc tgcttctact
     2701 ccttttgccg catgttcaaa tcgttcgatg ttccgtttca tttctgaata ccatgtggca
     2761 agtttcaatc caaaagtagt tggttcagca tggacaccgt gtgtccgacc catcatcact
     2821 gtatatttgt gttcttttgc tttttcgcca atgatttccg taaaacgttt caagtcttcc
     2881 cgtaaaatat catttgcttg cttgatgaga tagccataag cagtatctac gacatccgta
     2941 cttgttagac cataatggac ccattttctt tcttctccca atgtttcaga aaccgctcgt
     3001 gtaaacgcaa cgacatcatg gcgtgttaat ttttcgattt ccaagatccg atcgatatca
     3061 aatgatgcat gctcacgaat ttttttcaca tcttcttgcg ggatttcacc aagttctgcc
     3121 caagcttcat ctgctaaaat ctcgacttcg agccacgcac ggtatttgtt ctcttccgtc
     3181 cagatattcc ccatttcagg gcgtgtataa cgttctaaca tgttttttct cctctaatcc
     3241 caaatattcg tttgtttgat ttcttccagc gtttgctgga tgtcttctgt caagatagtg
     3301 atatgtccca tcttgcgacc tttttttgct tgtttttttc catagaaatg gaattgccag
     3361 tctggcttat tctcaatcaa gcgatagctt tccagcagtt gttctcccaa aacattgacc
     3421 attactgcat tgcttatcaa acgtactttt tctcctaatg gccaaccaca aatacctcga
     3481 atatgggcat cgaactgact catagaacaa gcttcaatag aataatgtcc agaattgtga
     3541 ggacgaggag ctaattcatt cacatatagc ccgcctgttt ttgttaaaaa catttctacc
     3601 cctaaaacac ctgaaaggct cactgcttct gcaatcacac gggcaatacg ctcagcctcc
     3661 tcgatcacat cctgatcaat cgcggctgga gcaatcgttt catgaagaat attgttgtgg
     3721 tggatgtttt ccacgaccgg aaaagtcgtg aaatctcctt gaccatttcc tgccaccatg
     3781 atcgaaatct ctttttcaaa cgggatccat gcttctaaaa cacaggttcc ttctcttaaa
     3841 aggtccatgg caggagctag atccgcccga cttttcaaaa catactgccc cttgccatca
     3901 tagccgcctc gagtggtttt caaaacacaa ggatagccga tcccgtcaat cgcatcttgg
     3961 atatccgtcg gactgacgat cgttgcataa ggagcaatca cgatgttatt cgtttccaaa
     4021 aaagattttt ctaacaggcg gtcttgcgta atcgcaagca agtctgtccc ttgaggaata
     4081 aatgacatgg gtaaaatcgc atttaacgcc tctacactga cgttttcgaa ttcataggta
     4141 atgacatctg ttcttctggc aagctctttc agcgcaaagg tgtcatcata gacactttca
     4201 atgtgccaat ctgcaacttg tgcggctgga caatcgctca caggatccag cacacctact
     4261 ttaaagccca tttcttttgc actaatcgcc atcatacgac ctaattgacc gccaccgacg
     4321 atcccgatcg ttgaaccagg caacaaaggt ttaaccaagt tgatcactac tttctataac
     4381 agttttagcc aacattgaac gtctttcagc caatttagcc tccaattcta aatcatacat
     4441 ggaaagcatc tgtatcgcaa gtagtccagc attaattgct cccgcttttc cgattgctgt
     4501 agtagctaca ggtactcctc caggcatttg gacaatcgaa agaagcgaat ccaaaccatt
     4561 taatgttcgt gattggaccg gtacaccgat aaccggcaaa gttgtttttg atgcgaccat
     4621 acccggcaga tgcgcggctc cccctgctcc tgcaataatg attttgattc ctttttttcg
     4681 agcgttttgt gcatattcaa acatcaaatc tggggttcga tgagcagaaa cgaccttttt
     4741 ttcataagac acaccgaatt catccaatat ttgacatgct tctttcattg tttcccaatc
     4801 tgaagtactc cccataatca cagagatttt tgctgtcatt gtttttcctc ccagtttttc
     4861 tacattcata ttttaacaat cacttttttt gatgtcaaag aaatttcgaa tgttaacaaa
     4921 gaaaaacatt ttattgttcg tgtttattaa agataaaaaa aaggaatgtg acaaaagtta
     4981 ggtcacattc ctttttatgg ataaacggtg ttctcctgcc aactcctcgg tcacaaggaa
     5041 caatattcaa caaacatgag aagctttttt ttgaaattgt tccttgtgac tcggagatga
     5101 acggcaggct cacgacctct tccttcaacg gtgttcaatc agctgaccct cggtattagc
     5161 tcaaacaaac ggcaaaatac gaggaactat ttttccgttt gtttctctaa caatcaggtc
     5221 aaaacgctga tttcacaacc tttcttttgc tctattactt cttgaaggct tcttccaata
     5281 tttcttcatc ttttttcaat ccattaaaga tcaaattcaa tatgatagcc gtaatgctac
     5341 tcattacgat gccatttcca gtgaacattt gcaatgtttg cggcatttga ctgaataatg
     5401 tcggcgtaac attgaaaccc agcccaaatc caacagaaac agcaacaatc aatagatttt
     5461 tatcattgct gaaatcaact tccaacagca tacggattcc ttggactgcc accatcccga
     5521 acatgactaa cattcctcca ccaagtaccg gctcagggat gatttgtgct aacgcaccga
     5581 ttttagggaa taatcctaaa acaatcaaga agaatgcaga gaaatagatg ggttttcttg
     5641 ttttgattcc cgaaagttga accaatccga cattttgaga aaagccagta taagggaatg
     5701 tattgaaaat accgcctaag ataacggcaa gtccttctgc acgatatccc tttttcaatt
     5761 cttcttctcc aacatttttc ccagtaatgt caccaagtgc aaaatataca cctgttgatt
     5821 ctaccatgct gaccatggaa atgatgatca ttagtaaaat cgaccatacg tcgaaagttg
     5881 gtttaccaaa ataaaagggt tgtggaacat ggaacaacgg cgcatctcca atcgcgctca
     5941 gatcaatcat tcccatgaaa gcagctaaga tgcttcctcc gactaaacca atcaataccg
     6001 cgattgaacg aatgaaccct tttgccagca tttgcacgcc tacaattagg agaatcgtca
     6061 caaatgcaag tagcagattt gttttgtcac caaaatcagc tgctgttgca ttacctccac
     6121 ccattttctc gattgctaca ggaatcaaag tcaaaccaat aacggtgata accgttcctg
     6181 ttactatagg agggaatagt tttttaattt tagaaaagac accagaaact aacacaacaa
     6241 agatcccaga agcgataata gatccgtaga tagctcctac tccttcattg gttccgatca
     6301 agatcagtgg agctactgct tggatcgcac atcctaaaac gacaggcaag ccaattccga
     6361 aaaatcggtt aactgtcaat tgcaataatg tcgctaatcc acacataaag atatcaattg
     6421 aaatcaaata agtcatctgt tcttgattaa agcctagtcc tgtaccgatc aaaagaggca
     6481 ccgctactgc tcctgcatac atcgctagca gatgctgcag ccctaaaacc gcagcttttc
     6541 cattttgtgt ttctttttcc acgattatgc gtcctcctca agaaactcaa cttgtccatt
     6601 cgtcaaggaa gcaatacgcg caagagaaac gactctcaat cccatttctt caagtaactg
     6661 acgtccatct tggaaagact tttcgatgac gatcccgatt ccttctactt gcgctccagc
     6721 ttgctggcat aattcgatca atccttttgc cgcttgtccg tttgccaaga aatcgtcgat
     6781 aatcaatact ttgtcttctg aagataaaaa tttacgagaa atagaaatcg tacttgtcac
     6841 ttgtttcgta aaagaataaa cagaagctgt caataattct tcatccatcg tcaaactttt
     6901 tgcttttcta gcgaagatca ttggaacatc taattctctt gcagcaaata aagctggtgc
     6961 gatcccagaa gcttcaatgg tcacaacttt cgtgattcct tgttctttaa atacttctgc
     7021 aaaccgtttt cccatttgtt ccatcaatac agggtctact tggtgagtga cgaagctgtc
     7081 gacttttaat acgccctcgc ctaatatacg tccgtctttt tgaatacgtt caactaattc
     7141 tttcacttta tttgctcctt tatcatttat taataccgaa ataaccgctt ttgtgcaaaa
     7201 acctactatg tttctgttaa agaaaaacac cctccacttt atcataagaa aagaagggtg
     7261 aaatagccaa tcttttactt atagtccagc atttacggta ctggctagaa actgtcatcc
     7321 atattatgac gatatataag taggtatgta aaactaataa tagtgtaaat tcattttctt
     7381 gcaactgttt tgcacgtgaa ggcagtttgc catatttgaa taaaaaatgt tgtttcaaat
     7441 attgaaaaca gtctaacact actttctgaa actgacaacg tggtttactt atttttttgt
     7501 aaaatctaat gatttttaat gcattattcc tttttgttcg ggtttaaata gttattttta
     7561 ttttctccta tctaaaaaag cagcccacat aaaaagaagg catgacaaaa agcatgtcat
     7621 agcccctttt tccgaataaa tagtgttcaa aagacagcct atcggcaata aggcaagatt
     7681 ttataaacta cgggaagtaa tttccgtaaa tctattctta ttcttttaag ctgacaagct
     7741 cgtcaatcac tactgctttt cctgaagcaa tagaatgatt tgctgcgatt cctgtcaaaa
     7801 tcgacaaaat accatctacg tgagttgctg cccgattcca cggatcttct tctttttctt
     7861 caccaaatag atcatctaat aaacgcggat ctcctccgcc atgtccgccc gcttcttgtt
     7921 cgacgattac ctcatatggc tcaccgaaca tcggatagac atggatggac tgaagaacgg
     7981 ctgctccttc atccttttta tctccacctg cattgatgta agacttttcg atcactttca
     8041 tttcgatccg tcctttgctg ccattgaaag aaacattgaa tccttcccac ggcatatacg
     8101 cgtttagcga atacgtcaaa atcgcaccag tttcatattt gaccgttacg cctagtgtat
     8161 cttcaataga gatatcatcg gaaaatacac tgcggtcacg aatatatcca ctgtcttttt
     8221 ctgcttctaa atacatttct cgcaattcct cattggctga caggtctata gcaaatggat
     8281 cagctttagc tagttcgctt ccatagcatc gactgtaaaa tatttctact ccgcgtttct
     8341 ccgcattatg catcccataa aagtttaatc caccgaatgc aaataccttt tggggacggc
     8401 tgttcaacca aaaattcact agatcaaaat gatgggtaga tttatgaaca agaagcccgc
     8461 cgctattgtg cttatttcga tgccatcttc tgaaataatc tgccccgtgt tgcgtattta
     8521 acagccattc gaagtggata gaaaacacat caccaatcac atcactttct atcagttcct
     8581 ttatcttcgt gtgatacggt gcataacgat aattaaaagt gactcgcagg cttcttcccg
     8641 tctgtttttg gaccgttatt atttcttgag ctttttcctc atcgatagtc agaggttttt
     8701 cgcaaataac atcatatcct tttttcattg cacgaatgat ataatcatga tgcgttcgat
     8761 ctactgacgt aataatgact tcagccggcc gtacgtcatc cagtagttgc tcaaattgtg
     8821 atggcataca tgtctttact tttttctgtc catacttgtt cacaagtatc cgatttgcat
     8881 aatccatcct tgtttgagat acatcacaaa aaccgactag ttcacaagtt tgatgatacg
     8941 tagaggcaat tgcctcataa aaaaaacgcg ctctcccacc aagtccgact tgtacatatt
     9001 ttttcttcat ttaaagtcat tctccttttc ttttaagcaa aatactgtga caaatggcag
     9061 ccctggatac agtaatgcag tgagtaaaaa atcatgctgt atacaccaga acagtccata
     9121 caaaaggcat aaataccaca gaaacccctt gacgtttttt gacaacagcc agatattcct
     9181 tttgatcaaa taaacaagac tatctgcttt atcttctttg cagatatcaa ttagcgtact
     9241 aaaaaaacta tgggatacaa aaagcaacag acacaataga aaaggaatca gataccaagc
     9301 tccaatcttt cccactaata aataccaatc aactgcaaaa ataatgacaa acagctgaaa
     9361 caacgtaatg atgaaacaca tccgtttgat ttttcctttc ctccaacata cctttttttc
     9421 tgccaagtaa tggacagata gataataaga aggatacatc atcaaaaaat aaatcagata
     9481 gacaacacga gcagacagca ctaatcctaa agtgaacaaa catcctagaa ctaaagcgaa
     9541 aagagcatta ctgataagac tggttaaaaa aatccgacag acatagtagt tgagcttgta
     9601 aagaaacatc tcttctattg gctttttcat attcagccct tcagaccgct tgtactgatc
     9661 ccctggacaa gatatttatt gaagaataag aaaatcagaa aaaccggtag cagcgacaac
     9721 gtagacatcg caaacatact cgcgtaagtc gtactagatc cagcgtctgc aaataatttg
     9781 atggcaatcg ataccgtgta agatctcggc ttgtttaaat aaatcaatgg tcccatgaag
     9841 tcgtcccatt tccaataaaa ctggatgatg atcgtcgtga tgatcgaagg tttcagcaaa
     9901 ggaaaaataa ttttggtaaa gacactatac ttgctgcatc cgtcaataat cgctgattca
     9961 tctaactctt tggggatgct gaccatgaat tgcatgatct ggtaaatgaa aaatgcttgt
    10021 ccaaaaaaat gtggtaaaat cagcggcaca tacgtcccaa ccaaattcag ccgattgtaa
    10081 ataataaact gcggaatcaa aatcacttgt gcaggaatca tcatcgttac taacatgacg
    10141 acgaaccaga atctttttcc tttgaaatcg attctcgata atgcgtaagc aatacaagtc
    10201 gagctcagaa ctgtccctat cgtcgaaact gcagagatga caaacgaatt cctaaagaaa
    10261 gtaaaaaatg aaattccagc aaaccccgcc catccttttg cgtaattttc ccattgaaaa
    10321 gtctctggaa tcaaattcag cgaaccactc ataatctctt gatccgtttt aaaagaacct
    10381 gcgatcatcc ataagaccgg ataaatcatg actagaccta atagcaaaat aaaaccgtat
    10441 tgaaaccatt tcgttactgt attattcatt tcttcctcct ctcattcaca caacgaccca
    10501 tttttttgat gatttgaaaa tcagaaaggt gacagctccc ataatcaaca gcatgatcca
    10561 cgaaatagca ctagcatacc ccatagaatt aaaagaaaaa gcttggttat acacataaag
    10621 agcataaaag ttcgtggcat cattcggacc accttgggta atgataaatg cctgcgtaaa
    10681 agtcataaac gccgcgattg tctgcataac caagttatac aaaatgatcg gcgaaagaag
    10741 aggtaacgtg atcttgtaaa acgtctgcca tttgttcgct ccatcgatct ctgccgcttc
    10801 ataatacgag cctgggatct ctttgatacc tgcagcaaag atgatcattg ccgaaccgaa
    10861 ttgccaagca gacatcaaaa caagagggat catgaccagt ttttgatcgc taaaccagga
    10921 aagcgcaggt gcgcctaatc gttccaggaa aatattcact aacccgtttt tagaaaacaa
    10981 ctcccgccaa actagagcga tagaaatact gccaccgatc aaactaggca cataatacaa
    11041 agaacgaaag aaagtcgttc ctttgatatt cctcgttaaa agataagcaa tcaatagacc
    11101 aaagatcagt ttcacaggta cagatgcaaa tacatagaga aaagtgactc ggatggagtt
    11161 caaaaaacgg gcatcactca gcatctttat ataattatcc aagccgatcc atgagactgg
    11221 atttcccaaa cgataatcgg tgaacgaata ataaagtgac aagatcatcg gtatcactgt
    11281 tatcaataga aatcctagga tgaatggaga agcaaatacg taaccgacca cattgtcttg
    11341 atccaagaac cgttttagtt tttctttttt catgccttcc tcctgctgct atcctagaat
    11401 gtttgacgca aactcataaa gactctctgc tgcttcttct gctgttactt cttcataaat
    11461 gactttttca actaaaagat tgtattggtc ttgaatttcc ggattcaaag cactatcaaa
    11521 gacattcact tcgccgttgt ccatatcgga aatcatttct aaataatcaa atgtcgcttg
    11581 caaagttgca tcatctgcac gagcgagctg ttctcgaata ttcgacatga ttggtacacc
    11641 acgttctcct tttaaaattt cattggcttc ttcactgttt tgaaagaaat caataaactt
    11701 agcagcttct tctttatggg ctgagctttt tgccatactc agcatttgcg aagaacgtaa
    11761 agggatacca gattctccat ctgcttttct gcgaggtgga ttaatcaact gaatctccat
    11821 atcttcagga gcccctgaca atatttctgt caactggtta cttgatagaa acaccattgc
    11881 cgcttctcta tccctaaccg cctgaaaaga cagatccgtc gtacttgcag ctccaccacc
    11941 tggttctgga tgggcccctt tgtctaccag atttttacgc agctggaaaa aatctgttag
    12001 atagtctgga ttttcaaatc ccaagctgtt agatagatct tcccgattat aaaaattcaa
    12061 tcctttctca acttgtggta tttgaataat acagccagct ataaaatctt cgaatcttga
    12121 aatagcatag attcctaatt cttcagtgat ttgatagctg atttgttcaa attcatccca
    12181 tgtccaattt gaagctggtt cagggatatt taattgttca aatatatttt tattgtaagc
    12241 aatcgcaaac gaattcaccc cgttagacag tccaactaat tgcccgtcgt aagtcgtcgt
    12301 tgctaaatag gattcatctg cattagaaat atcaattatc ccttgctcca catattcatc
    12361 taatggttca atctgattga tgtattgagg aaattgattg ttcagttgga aaatgtccca
    12421 gatatcatct gatgctgcta gtgtacctaa ttttgtgtaa tatccttcat aatcataaaa
    12481 ttcataatcg atcgtcacat ggggattttt ttcttcataa agctcgatta cttttactgt
    12541 tgcatcgtgt cggacttgcg aaccccacca agccatgttc agcgtgattt ttttgtcatc
    12601 ttcttgccca caagccgtca agaagaataa cataacactt aaagctaaga tgattctcat
    12661 tcttttcttc attctctgca cctctacttc cattgttatt tttcaatata ctgtattttt
    12721 atacagaaca tatttattaa acgatctttt tcaaggggaa taaaaaccaa gacttgcctt
    12781 atcgttctgc ctaggatgaa caactcaata ctttcaagaa aaattagata gtgctgattc
    12841 ttctttcatc gctaaaaaca cttcttgctt caatgatggg aggataatta cgcaaagaaa
    12901 aaaggatatc agacaactgc ccaatatcct tgacatagct aaataagcat gcttcttcgt
    12961 gtgcgctctt ccctaaaaaa cagaaaaata actagtaatt ctgtatgaaa ttaggtacaa
    13021 tatacacgtc gatgcacaag cattgactat ggcaacttga ttggtcagtt cccgctgact
    13081 tttcaagccc cgacaattta gctgctacta aattgtcggg tgcctttttt agtttttatt
    13141 cgtttttcat tctatagaaa gcgcttcaat aaatcaagac atttttttgt tagaatgcta
    13201 ataataacaa aaggttttaa ctcttcttat gtacattaca ggtaaaaaaa caaaaaaatt
    13261 tctctttttt ttgaattctg aacaaatagt actggagtac aaaagacgag gaagagccac
    13321 tatctgctct tcctcgtctt ttgattctca cctgttattc atgattcttt ttctcagttt
    13381 cccgatattt ttccatttta cgactaaaaa gctctccgct acttggtggc gtatatgtga
    13441 tggcatttgc tcctgcttga atggtctcag caatactttc ttccgttgga ccacctgtcg
    13501 caatgatagg aagttcagga tattttttac gaaaatggcg tactgcttct gcagtttctt
    13561 tacctgcact gatattgata atcgaaatac cagctgctaa cttttcatct atttctgtat
    13621 atttcgacgt taccgtactg ataactggaa tatcaactac tcgacaaact tcttttactg
    13681 tctctaaagg cgtaggaccg ttcaaaacga cccctaacga accttgcgct tctgcgaaca
    13741 tgctcatgta ggaagaacga gcaccttgtg tcaaaccacc acctacaccg gagaaaactg
    13801 gaatgtcagc tgcctcaata atgcttttag taatcgctgg atgaggtgta aaaggataca
    13861 cagcaattac tgcattcgca tttgtattac gtatgatcgc aatatccgta gtaaagatga
    13921 tcgaacggat caattttcca aatatcttga tcccgcttgc ttctaaaatg atttccggta
    13981 ctcttacgat atcttttctc aattccgtca tgatttctgg cgtaaattgt tctgccatca
    14041 gccacttctc ctttgctatc tccggtcata ctgattcaat tgatacgtct cgatgacctg
    14101 gatgattttt tgcgcataac cggggtctgt cgcataacct gcatcttgca atgcttgtgc
    14161 ggcttcctga tagttggtgg ccgtaatcac tttttcatac agcgcaggat tccaatctac
    14221 tccttggacg aacagttgtg tatggtcatc catggactgt tcccatgaat catacacttt
    14281 gaagtctcct tggatagtta tccattcttc gttaacaaat tcctttgttt ccaggcttac
    14341 tttcttctga tccccataag ccttgatccc gaaaagatta ttgtatttgc tagccaaggt
    14401 actttttccc caatttgatt ctaatatagc ttgtcctaaa atgatactag gaagcactcc
    14461 ataaccatct tgtagttctt gggcatgcgg aacaagccgt tcaataaatt gctgatggga
    14521 ttgcgcttca ttttcttgct gattatcagt aaaaggagaa gacagattct tcaaggaaaa
    14581 aacaaaggct aagagtatca ctgctacacc ggcaaccact gctggccaac ggatattctt
    14641 gcgcttttgc tttttccact tttttttcgc catgatcaat tcctactttt catttaattc
    14701 attcagatat tcttctagcg tcaattggtg tttggtgata tactcagcgt tttctttacc
    14761 gacataacgt aagtgccaag gttcatatgt gatcttagtt atatcttcgt gtccttttgg
    14821 ataacgaacg ataaatccat actgcgcagc attatctgcg atccatttag ctccaggctg
    14881 atctccgtaa cttgcatcca atacagtaga aggataactg ttgtaccagt tttgatcgac
    14941 gacatcgact gccagacctg tgtggtgttc gctgtatcct ggctccgtaa tcgtttcttt
    15001 agtaatcttt gtcgcctcct cttctgatac accttgacga ctcatcacat cttggacatt
    15061 ttgttcgaaa acttgttgtt gggatgatac agaccggtaa gctgagacca tcactaatgg
    15121 aaatccagct gcttcagcag ccttggcaaa tgcttcatat tctggagcaa tgcgtttgtc
    15181 gatcaagtat ccgttactta atgatgccaa ttgagtagac tcatcaattt ctttttctaa
    15241 tttgtgatct ggaccaacca aaactagctt ccaatcgtct gttttaacat ctggcaattg
    15301 tttggacgta cgatcttttg tttctttgtc ctttaccttg ctgctggttg tttgactgtt
    15361 cttttcttta ctagcagttg ttttggtgct tgttcgttcg actgcttgat tgccggcttg
    15421 aacatgcgaa tcatcatcgc taaaaataac atagcccatt gctgcgatca taatcacaac
    15481 tgctccaaat cctaaaaatt tattcacttg aaaaatcctt tcgttgttct tcacgttccc
    15541 cattcgcctc atgaacgtaa gtttcttcca gattttcatt cacccaatcg agaagttcga
    15601 ttttttctcc ttcgatctgt ttttgaacat ctgctggcag acgaaaattc aaaatatcat
    15661 catagcccca ttcatcatat tgaatactta ctttcaagtc aaggtatcta ccagcctgct
    15721 gattattttt caaagggatc agctcgactt ttgctgcttt gctgctaagc catttttgcg
    15781 cgatgcgtgt tttgatttgc ttgtactctg caacggcaac gtttcgtttt tcgggaaaaa
    15841 taatcgtttg acgcaaaatc ttttgcgtta gcatccattc ataatcagtg aaaagatttt
    15901 ttactttctg catcgcttca gatggcagat ttttctcgcc atctttccat ttttgccatt
    15961 gttctggact gatcagcaag tattcatcta aaaaattttt ttcagtttca aactgttcta
    16021 ataatacact cataataaga tcaatatata cttgatgcac gtttatcccc tccttttaac
    16081 tattctactt aagttttgat caaaaagcac ctcttaaatc gctgctttca ttttttttaa
    16141 tgtttcaagt cctctcctct ttattcttcg tgatttttgt tataataata gacagacaag
    16201 aaactcaatc caactatgaa ggagcgtttt cagatgaaaa aatatcaatg gggaatcgtt
    16261 ggtcttggta ccatcgcaca tgagtttgcc gaaagcttca atcaagagac gagcgaacta
    16321 acagcagttg cttccagaac ttcagaaaaa gcggaaaact tcgctcaccg ttacaatatt
    16381 ccaaaagctt acggaagcta tcaagaaatg ttagacgatg ctgaaatcga catcgtgtac
    16441 attgctgtac caaatagaca gcatatcgac catatcttgg cagcattgga agcaggcaaa
    16501 cacgttctat gtgaaaaagc catcacgatg aataaaaaag aactggctga cgctatgcgc
    16561 ttagcagaag aaaagaattt gatcttagca gaagcgatga caatcttcaa catgccactt
    16621 tatcagcagt tacgctcaat aatggataca ggaaaactgg gggctttaaa aatgatccaa
    16681 gcacctttcg gcagttacaa agaaccagac cctaaaaacc ggttcttcaa tccagaatta
    16741 gctggaggag ctctgttgga tatcggtacg tatgctgtat ctttcgctcg tttctttttg
    16801 agctctcaac ctgaagtagt tgcctctaca atggttcctt ttgaaacagg cgtggacgaa
    16861 caatccgtca ctatcttgcg taacaaagaa aatgaacttg ctgctgtcag cttgactttt
    16921 caggcaaaaa tgccaaaagt cggtatcgtt gcctttgaga acggttatgt gacgattgct
    16981 gattacccgc gtgctgatcg tgcggagatc ttatttacag atggtacaaa ggaattcatc
    17041 gaaagcggga atacatctga agcaatgaat tacgaaataa agaatatggt acacatgatt
    17101 gaaggtgact tgcccaatcg ctctctcttc ttgacaaaag atgtcatcga aatcttggat
    17161 caaatgcaag ttctatggaa aaatatgtaa ttttaaaagg ttgtgaaaaa gctgttctgc
    17221 atcaagtaat aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggctggact
    17281 tattaccgca gaggaaagct tttgaacacc gtttaagaaa gaggttgtga gcctaccgtt
    17341 cagctccgag tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgaatattgt
    17401 gacttgtgac cgaggagttg acaggagaac accgtttaaa gaagaggttg tgaaatcagc
    17461 tttttgacct gaatattagc tgaattcaca acctctattt ttggattttt tgttgggaga
    17521 attctgcctt gatttcaata aaacttttaa aagactgcaa aaactggaac aattctgctc
    17581 gatcctcgaa ttcagggcga ctagtcggca gaggcatttg ccgatacaat acataaactt
    17641 cttcaatacg tgttaaaatt tgtctgccat catttgactc tgaaaatgtt tctgcagtat
    17701 aaatcaataa accatagata tgttttccat aataagctgg ttgctggatt ctctctagat
    17761 tttccagcat gtctttaatc acattggcct gtgctcttct catagagaag taagtttcgt
    17821 agtatagcgg ctgtctgagc cagtagtttt cttgatgttc tctggcatct tcttggcttt
    17881 cgcgaatgaa tgtcagtaaa tgttcgcatt tgatctgcac atcttcttta ttttctgata
    17941 aaagaaattc tgccatttgt ctcaaaatat ttcgaaattc tttttcgatc tgtatttggt
    18001 tttccttcag ctgcttcgta cgatctggca tgtaaacatt tgcaagcaaa gcaaatacta
    18061 atccaatcgc cattaatccc gcttcattgc cgatcagctg ccaagacatg ttttcctcca
    18121 ctaaataatg agtgaccaaa accgaattta ctactatccc ttcggttaat ttgaagcgag
    18181 aggcaagcgg gatgaacagg aataaaaaaa gactgaagcc aaaaatagta tacccaacag
    18241 caaaaaaact gaacagtgct gttgccgtag ctaatataaa ggctgcgatt cgattcatgc
    18301 ccgtacgcaa tgtagagcgc ttggtatttc ctacacttaa taatgcaatg atccctgcag
    18361 ctggccaata caacagcgat aagctacccg ctgtgaccat cgaaaagaag acactgatca
    18421 ctgttttgac tgttcgcatt cctatacgca tttacttccc ccttttttat tttaattata
    18481 cttgaataat gaaaaactag ctatcagaca aaagtttagg tatgatagta aaaagaccaa
    18541 aaaggagaat ctctatgcaa tttggccgtt ttcgactagg tatgcgtacg atcaaaagtg
    18601 ccttagctgt ttttttatgt atcttgtttt ttcatttgac tcaacgagga atcccgatga
    18661 ttgctgcatt atctgctgtt ttttctttgc gtcaggactt gactacaacc gtttcttttg
    18721 gaaagtctag gatcatcggc aatacattag gtggtacatt agctatcatt tatttttttg
    18781 tcaaaagcta ttttcacaat gattttttgg tagaattgat cttgcttcct attctagtta
    18841 ttattgtgat cgtcgtgtca gatggtatta acaataattc cggtatcatt tccgcaatag
    18901 ctacgttgtt gctgattgcg ttgagcatcc ctcaaggcga atccgctttg tatgctatcc
    18961 aacgtgtact agatactttt atagggacat tcattgcaat cggtatcaat ttctttcttc
    19021 gtcctcctga aacagaaaag aaggaagaac tatcagaaga tttggtcgaa ctccaaaaga
    19081 aagaacgatt gctaagaaca aatttacaag agatagaagc taagatccaa aaacaaaaga
    19141 aaaatagcga ggactaattt tcttttgcaa taaaataaaa aaatggagag aagaacttta
    19201 aaaagtcttc ctccattttt gattgaacgg tgaagtgtac tactttattc ttccttcatc
    19261 gagttttgtt ctgcctcttt tggcgcttca tacatattta cttggctagt agtcccacgt
    19321 gtcgacaaat tctctacgat ttccttcaag tcaagtcctg ttgtcgcttt tagtgtttct
    19381 tgagtagaag aaagcaaatt tgtggcataa tttgtcactc ggttcgctcc actggtttct
    19441 cctgaactgc ttcctgtatc gacaacagag attttttcga tatttcctag cggctgtgct
    19501 gcttcgcgca tcaattgcgg tagcatatcg ataaccatac tcaagacagc tgcttcgcca
    19561 tattccttga aggcattggc gatcttttgt tttgcttcgg cttctgcttt tccttttgct
    19621 agtgctgctt cggcttctgc ttgtccagct aaacgtgtct tattggcttc cgcttctgcc
    19681 agtgcttcca cacggaatcg ttctgcttcg gcttcagtca cttctttgac tttttgagct
    19741 tgtgcttctt gttctttagc gtaccgatcg gcatctgctt ttttcttcac ttccgaatca
    19801 tattgctttt cacgacgtgt gatttctttt tcttccagtt cgatttgttt ttgacgttct
    19861 acgattttaa tctgcatttc ctgttcgatt acttgttgtt gtgctcgggc actttctaaa
    19921 ttgtaggctt ggtctgcttt tgctttagca acatcttgtt cttgtttgta ggttgctaat
    19981 ttcagttctt tttcttttaa tgattctgcg atctctgttt gtcgctgtaa ttcagctgct
    20041 tgtgattctt tttctgcttg agcttttttg atacgcgttt cttttagagc ttccgcttct
    20101 gctatatctg catctctttt gacttgggcg attcttggtt tccctaaaga gtcaagataa
    20161 ccgtttttgt ctctgacctc tttgatagtg aaagaaacga tgatcaaccc catttttgca
    20221 aggtctacag aagcgacttc ttgtacgctt tgactgaact tatcgcgatt ttgatagatt
    20281 tcttctacag tcatagaacc aagaatcgag cgcaagtgtc cttctaatac ttctcttgct
    20341 tcattttcca gttcttctct tgtcttgcct aaaaactgtt ctgctgctgt agcaatctct
    20401 tctacagatg aaccgatttt gatgatcgaa gtaccatcac acatcaccgg tacgccttgt
    20461 tccgtataca cttcaggggt ggagacgtcc aatttactcg ataatagact gatccgattc
    20521 gaacgttgaa aaactggcaa gacgaatgcg ccacctccac gcacaatttt gattttgtta
    20581 ttgctttcat caatgtgcac atttttgctt cctaaataac ttccactgat gatcaacgct
    20641 tcatccggtt ttgctgtctg gtacttcgct acaaagacga tcagcaacat cagtaaaatc
    20701 aaagctgcga ttgcgatcgc gataaaaaca actggtattt ccatttaaca cacgctcctt
    20761 taagctttta tcatgctttc ataaggcatg acataacaga ttctttcctt gatctcaatg
    20821 atcaatactt tcgttccccg ctcagctgtc tgttcttggg ggctgtaaaa acaagctgga
    20881 cgactaatgg aaccggtaac acttttcaac tgaatttctc ccatacctcg tacagggatc
    20941 ggtgtgatca caacagctac ttgtccttcc agagtttttt cagaagacga catcgttgat
    21001 tcggcatgtt tcatcggcat caatatatag aaattaagca aaaataccaa cactatcgat
    21061 atgcctccac tagcaataaa taaaaataaa gatgattgat ctgttaaacg ctcacctaga
    21121 aaaccaaata agcttgtgaa agttaaccaa gggataataa gcatcggatc aagcgggcca
    21181 tcaaaatcga atacatctcc aaaaataatc aataggactg ccaatcctgc acaaatcagc
    21241 aatgtatacc aataaatcgt ctcaagtgcg attcccccta tcatatacac gcctccttcc
    21301 atttctacgt ctatcatacc aatgtttgaa aattctgtat agcttctttt accgactcag
    21361 tggtgttttt tataggaagg ttgtgtttat cgttgacaat ttgttaaatt tttgttttca
    21421 tgttaaaatt ttcacaataa attctttcga ggtagcctat gtttcctttt tttaagaaaa
    21481 aaccattaaa gctccagaac acgatcatcc ttgtcgtttg tttctccaca ttcgtttctt
    21541 tgattgtttc tgccatattg atcaggaatt ttattctttc gatgaaccga tctagtgtgt
    21601 cagataaaat ctctagcatt gctaaaatcg ttgccaatga taaactcatc caaaaaaagc
    21661 tgcagactgg tgacccgaca catgcgattc aaggaataac aaatgaaatc atgattgcat
    21721 cgaatttaga ttttatcgtt gtcatggacc ctgaaggcat tcgatattct cacgcagacc
    21781 caagttacat cggtcttcct ttctcaagtc atgaagatgt gaaaaaagtg attggaggac
    21841 aagaattttt ctctgaaaaa gaaggaatgt tagggttcgg cttccgctat ttcgtcccta
    21901 tttattatga tcaagagatt attggtgcag tttgtgttgg cttaacaaac aacacgattg
    21961 acaatcaagt caaagatgga caaaaaatga ttgttttttc attgattatt ggacttagcg
    22021 taggcatcat aggcgccgtt tttcttgccc aaaaaatgaa aaaagtactg cttggacttg
    22081 aaccacaaga aatagcagaa cagttaagtg aaaaaacgat tatcgaaaat gaagtagatg
    22141 aaggaatctt agccatctct tctgataaaa ctatcctctt gatcaataca cctgcaaaaa
    22201 acattttcta ccaaacagag cagctattgg atttcgaagt aggaaaaaaa tcagtgataa
    22261 tctatatgac gttttgttta aagagacgat tgagcatcaa acgaccgttc ggactcaatc
    22321 catgtattta aatgggatag aaactatttc tagcgttaca cctcttttta gcaatgaccg
    22381 ttttttagga gcagtcgcaa ctttacgaaa tcgatcagaa gtaatgaaat taatggagga
    22441 gcttgggggg acgaaacaat atataaactc catgagagct caaacccatg aattcatgaa
    22501 taaacttcat gtcatttctg gtttgattga tttgaagaag tatgaagaag tcacccattt
    22561 tatccagcag ctaaatcaaa attataaaaa agagattggc caagtctcaa gtctgatcaa
    22621 agtacctgca attgccggtt ttttattagg caaaataaac gaagccaaag aacaatctgt
    22681 tgaaatgctg ttagaaagcg attcttttat ccctaaaatg gagatggatg actccatcca
    22741 ttcacttttg caagtgttag ggaacttact tgataatgca aaagaagccg tgctacaaaa
    22801 acatgaaaga aacggacagg tccggattca tttaaactat gacacagaag ctgaagtatt
    22861 catcgtgacc gtctgcgatg acggtgtcgg gatatcagaa gaaatctcag aaaaaatatt
    22921 tacgctaggt ttttcgacga agggaatcaa tcgtgggttt ggattgaatc tcatccattc
    22981 aatcgtcaaa cgccacggtg gatttttaga attttccaaa caaacaaatg gcggtacaat
    23041 cgtctatcta gaattaccaa aaaaggaaga tcaaaatgat gacaaatgtc ctgataatcg
    23101 aagatgatcc aatggttgca gaattgaatc gtacttttgt ggaaagcatc cctaatatga
    23161 acgtaatcga aagtttcaga aatgtagaag aaggaaaaaa atatttcaga caaaaaaacg
    23221 cagatctgct gctgcttgat gtatacatgc ctggcgaaag tggtatccat tttttatctt
    23281 ggcttagaca gcaaggaaat gatatcccag tgattttgat cactgctgca gatgatatcg
    23341 aaaccataaa gaatgtgatc cgctacggtg ccttagatta tttgatcaaa ccgttcactt
    23401 tgcaacgttt tcagctggcg atcgaaaagt atcttgcttt ctctcagaca atccaaacga
    23461 ctgaaaaagc cgatcagaaa ttgatcgatc aagtattcaa ccacaccact cccattcacg
    23521 aaataccatc attcatcgat ttgcctaaag gtctgtcaaa actgacttta aaaaaaataa
    23581 gtgcatttat tctaaaagaa gatactattt tttcaacaaa agaactggct caaaaaagct
    23641 cactgtcacg tatatccgtc aagaaatatt tgaatttttt agtagaacaa gatatattga
    23701 acgaaagaat caaatatttg gaaatcggcc ggccgctaac ctgttatgaa ttgacaaaga
    23761 atgggcggaa aaagctcaca gattactgtt gagtcatcga attatcccat acaaaatagt
    23821 ccaagtatac agaagtaaca actgtttact tggactattt ttaaacattt tattgacgaa
    23881 tatccatgtt ttcttatctt aattgacggg ctttaggaag agatacctca tttttcgttt
    23941 ttccagtttc tattatttca cgaaagttct cgaaacttgt cgaaatcata tttgataatg
    24001 cctcatctgt atttgaacct acatgcgggg ttacaagaac tctagggtac atttctacta
    24061 attgctggat agcagggtct ggaatcgtct cccatggctt gaaggatcgg aaaaaaacct
    24121 ctgtttcatt tgcaaaaaca tcggttgcaa aaccagctaa atgattgctt ttcaaagctc
    24181 ttaaaatagc ttcattatct tgaagctctc cacgggctgt attaatcaag atgctacctt
    24241 ttttcatttg agctaaaaat ccttcattga tcatttgatc gttttcacct gggatatgag
    24301 gtacatgaat actgacaata tcgcttttgg ctaataactc ttcaagcgag tggaacgtca
    24361 atactttttt agcagcttct gtctgatgaa tatcgtagcc gatcactttg gctcctagtc
    24421 ccttaaacaa aacagcttcc gtcaacccta tcctgcctgt tcctatgatc cctactgtgc
    24481 aatttctgat ttcacgacta aacatttgct catccactcg aaaatctttg aaagatgttc
    24541 ggatcgtcgt atacgcagta tttctcaaaa gcatcatggc aaaagtcaaa gataactctg
    24601 ctatcgcatt tggcgaatac gatggtacac gggctactgt cataccaaaa tcagctgccg
    24661 cttgtagatc gatatgattg actcccacag tccgtgtaaa aacataacga atcccatatt
    24721 cctgcatttt tgcaagattg gtctcatctg ctatacaatt tcccctcaac aatacagcat
    24781 catggtcctt tgctgacacg atattgtcat gtgtcaacag ttcttcataa agactgagat
    24841 cataatggta aatattcaat ttttcaaaaa aatctatctc gttcggacga acaccgtaac
    24901 atgcgattct catactttct tcctccattt cttatgaaaa taaacctatg ccatgaatca
    24961 tttgtaatac aacgatccat acaggtgtca gtaccacagc aaaaatagtt gacagtaagg
    25021 atgcattgga ggctaaaagt gcctccctat caaaactgat agcataggct gctgccactg
    25081 ttgcagctgg tgtagccatc atgataacga ttgttgccag tgcttcaaaa ttgactggta
    25141 gaatattcgt tgccgtcaaa actgctagaa tcacgatatt caatgcaggt acaaatacca
    25201 ctttgactag actgtaatac catgaagttt tttcagatgc agcttttttg aagcttactt
    25261 cacctaatgt tgcgccgatt gctagccacg ccaatggtga acttaaacca gctaaataat
    25321 ccatcgactg aaatagccac ggtgcagtct ggtcaattcg caaaaacgct acttggctta
    25381 tctctccttc tgcacctgta actgacactt gtggcagata cccttggaac agccaaatga
    25441 aaaggccggc aaaagtagcg ataatgattg gattgacgaa catcgctttg atatttttag
    25501 cttccatctt caagccactc atttttatgt atccataaga atacaaaaag attcggtagc
    25561 caatattgaa aattgaggaa tacatcacgc cgacaggacc ataaatcgca ctgacgatag
    25621 gcgttccgaa aaaggtcgta gaaccaaaga tcgtcaggac acgcaacgtt gtttgcttat
    25681 cccctttata gcttaagaaa aaaggttttg aaactagaat aagaataata taaataacta
    25741 atccccaaat caagacattg attccttgca tcagtaattc tgtattgata tcttgcatga
    25801 aagctcgaaa tgccagtgct ggcaaagcca ctgttaagac gactttcgat agaatttttc
    25861 caacagattc agaaaaaatc cccttttttc tacaaacaaa ccctaataat ataataaaaa
    25921 ctgttgaagt gattgcactg acaatcgaca tatctgtcaa agtcgatcga aaaatgtcaa
    25981 ataagttcat ctttctcctc ctaattatgt tcatttattc acaataaaaa aagcttttat
    26041 tttgctaata cgtattaaca tttttaat
//
LOCUS       DAYYBH010000036        25212 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000036 DAYYBH010000000
VERSION     DAYYBH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.036"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACHAUG_001640"
     CDS             complement(164..388)
                     /locus_tag="ACHAUG_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8480733.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACHAUG_001641"
     CDS             complement(586..2166)
                     /locus_tag="ACHAUG_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8480734.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACHAUG_001642"
     CDS             complement(2567..3943)
                     /locus_tag="ACHAUG_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8480735.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACHAUG_001643"
     CDS             complement(4071..5159)
                     /locus_tag="ACHAUG_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8480736.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACHAUG_001644"
     CDS             5331..5765
                     /locus_tag="ACHAUG_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8480737.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACHAUG_001645"
     CDS             complement(5843..6376)
                     /locus_tag="ACHAUG_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8480738.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACHAUG_001646"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACHAUG_001646"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8480739.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACHAUG_001647"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACHAUG_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8480740.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACHAUG_001648"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACHAUG_001648"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8480741.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACHAUG_001649"
     CDS             complement(10101..10679)
                     /locus_tag="ACHAUG_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8480742.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACHAUG_001650"
     CDS             11001..11471
                     /locus_tag="ACHAUG_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8480743.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACHAUG_001651"
     CDS             complement(11579..12805)
                     /locus_tag="ACHAUG_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8480744.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACHAUG_001652"
     CDS             complement(13559..13759)
                     /locus_tag="ACHAUG_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8480745.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACHAUG_001653"
     CDS             complement(13893..14501)
                     /locus_tag="ACHAUG_001653"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8480746.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACHAUG_001654"
     CDS             complement(14586..15056)
                     /locus_tag="ACHAUG_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8480747.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACHAUG_001655"
     CDS             complement(15104..16018)
                     /locus_tag="ACHAUG_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8480748.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACHAUG_001656"
     CDS             16164..16967
                     /locus_tag="ACHAUG_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8480749.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACHAUG_001657"
     CDS             17145..18092
                     /locus_tag="ACHAUG_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8480750.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACHAUG_001658"
     CDS             complement(18160..18453)
                     /locus_tag="ACHAUG_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8480751.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACHAUG_001659"
     CDS             complement(18481..19146)
                     /locus_tag="ACHAUG_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8480752.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACHAUG_001660"
     CDS             complement(19286..19918)
                     /locus_tag="ACHAUG_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8480753.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACHAUG_001661"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACHAUG_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8480754.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACHAUG_001662"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACHAUG_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8480755.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACHAUG_001663"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACHAUG_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8480756.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACHAUG_001664"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACHAUG_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8480757.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="ACHAUG_001665"
     CDS             23883..25052
                     /locus_tag="ACHAUG_001665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8480758.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         7575 a         5086 c         4059 g         8492 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ct
//
LOCUS       DAYYBH010000037        24544 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000037 DAYYBH010000000
VERSION     DAYYBH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.037"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACHAUG_001666"
     CDS             complement(55..789)
                     /locus_tag="ACHAUG_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8480759.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACHAUG_001667"
     CDS             1116..1526
                     /locus_tag="ACHAUG_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8480760.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACHAUG_001668"
     CDS             1519..2220
                     /locus_tag="ACHAUG_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8480761.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACHAUG_001669"
     CDS             2220..3806
                     /locus_tag="ACHAUG_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480762.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACHAUG_001670"
     CDS             3825..4046
                     /locus_tag="ACHAUG_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8480763.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACHAUG_001671"
     CDS             4043..4804
                     /locus_tag="ACHAUG_001671"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8480764.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACHAUG_001672"
     CDS             5184..5693
                     /locus_tag="ACHAUG_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8480765.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACHAUG_001673"
     CDS             complement(5768..6307)
                     /locus_tag="ACHAUG_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8480766.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHAUG_001674"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHAUG_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8480767.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACHAUG_001675"
     CDS             7320..8171
                     /locus_tag="ACHAUG_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8480768.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACHAUG_001676"
     CDS             8199..8834
                     /locus_tag="ACHAUG_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8480769.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACHAUG_001677"
     CDS             8854..9627
                     /locus_tag="ACHAUG_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8480770.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACHAUG_001678"
     CDS             10035..10832
                     /locus_tag="ACHAUG_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8480771.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACHAUG_001679"
     CDS             10810..11223
                     /locus_tag="ACHAUG_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8480772.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACHAUG_001680"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACHAUG_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8480773.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACHAUG_001681"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACHAUG_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8480774.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACHAUG_001682"
     CDS             16000..16560
                     /locus_tag="ACHAUG_001682"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8480775.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHAUG_001683"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHAUG_001683"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8480776.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACHAUG_001684"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACHAUG_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8480777.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACHAUG_001685"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACHAUG_001685"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8480778.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHAUG_001686"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHAUG_001686"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8480779.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHAUG_001687"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHAUG_001687"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8480780.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACHAUG_001688"
     CDS             21907..22485
                     /locus_tag="ACHAUG_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8480781.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACHAUG_001689"
     CDS             22537..23019
                     /locus_tag="ACHAUG_001689"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8480782.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACHAUG_001690"
     CDS             23136..23510
                     /locus_tag="ACHAUG_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480783.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23885..>24544)
                     /locus_tag="ACHAUG_001691"
     CDS             complement(23885..>24544)
                     /locus_tag="ACHAUG_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8480784.1"
                     /translation="VSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSR
                     GVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKA
                     VYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLI
                     ESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFT
                     E"
BASE COUNT         7983 a         4406 c         5248 g         6907 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgggagcgt caataatttt gtgtaaataa attgtcctcc tgcaaaataa
    23881 ttagttactc agtaaacatt gaaactaatg tatcggttac ctgttgaaaa cctttatggc
    23941 ttctgtttag aaatttttga ttgtatgtat caaaaatgct gactagaaag cgttctagtg
    24001 attcttcatt ttgaaactgc tcttttctac ggctgtatct tttaatttgc ttattgaaag
    24061 actcgattag attggttgag taaatggttc tacgaatgct aggtggaaaa tcataaaaag
    24121 ttaataagtc ttggttttct atgagtgact gcgtcacttt aggatagttt ttcttccatt
    24181 tctcaatcat gccggataag aaggtattcg cttcttcttt tgagttagct tgataaacag
    24241 ccttaaagtc atcacagatt tcttttcggt ctttgacacg tactttatga gcgatgttac
    24301 gagatacatg gatacaacaa tgctgatatt ttgctttagg ataaatttga tggatagtat
    24361 ctttcatgcc ttttaagccg tccgtaataa aaagcaagac ttcttgaact cctctggagt
    24421 taatatcctg tagcagctca ttccaaacgt atgttgattc agttggagca atcgcataac
    24481 tcagtacttc tttagtgccg tcttctcgta taccaatggc aatataaatc gcttctttgg
    24541 atac
//
LOCUS       DAYYBH010000038        24337 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000038 DAYYBH010000000
VERSION     DAYYBH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.038"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..2477)
                     /locus_tag="ACHAUG_001692"
     CDS             complement(132..2477)
                     /locus_tag="ACHAUG_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8480785.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="ACHAUG_001693"
     CDS             2756..3658
                     /locus_tag="ACHAUG_001693"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8480786.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="ACHAUG_001694"
     CDS             3869..5644
                     /locus_tag="ACHAUG_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8480787.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="ACHAUG_001695"
     CDS             complement(5710..7296)
                     /locus_tag="ACHAUG_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8480788.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="ACHAUG_001696"
     CDS             7547..7771
                     /locus_tag="ACHAUG_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8480789.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="ACHAUG_001697"
     CDS             7913..9664
                     /locus_tag="ACHAUG_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8480790.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="ACHAUG_001698"
     CDS             9664..11448
                     /locus_tag="ACHAUG_001698"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480791.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /gene="licT"
                     /locus_tag="ACHAUG_001699"
                     /pseudo
     CDS             11783..12635
                     /gene="licT"
                     /locus_tag="ACHAUG_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12732..14618
                     /locus_tag="ACHAUG_001700"
     CDS             12732..14618
                     /locus_tag="ACHAUG_001700"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8480792.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="ACHAUG_001701"
     CDS             complement(14659..15561)
                     /locus_tag="ACHAUG_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8480793.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15791..17194
                     /locus_tag="ACHAUG_001702"
     CDS             15791..17194
                     /locus_tag="ACHAUG_001702"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8480794.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17194..18627
                     /locus_tag="ACHAUG_001703"
     CDS             17194..18627
                     /locus_tag="ACHAUG_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8480795.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18772..19698
                     /gene="trxB"
                     /locus_tag="ACHAUG_001704"
     CDS             18772..19698
                     /gene="trxB"
                     /locus_tag="ACHAUG_001704"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8480796.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19911..21638
                     /locus_tag="ACHAUG_001705"
     CDS             19911..21638
                     /locus_tag="ACHAUG_001705"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8480797.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21654..22004
                     /locus_tag="ACHAUG_001706"
     CDS             21654..22004
                     /locus_tag="ACHAUG_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8480798.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22098..22958)
                     /locus_tag="ACHAUG_001707"
     CDS             complement(22098..22958)
                     /locus_tag="ACHAUG_001707"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8480799.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23050..23478
                     /locus_tag="ACHAUG_001708"
     CDS             23050..23478
                     /locus_tag="ACHAUG_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8480800.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23640..23816
                     /gene="rpsU"
                     /locus_tag="ACHAUG_001709"
     CDS             23640..23816
                     /gene="rpsU"
                     /locus_tag="ACHAUG_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8480801.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23843..24289
                     /locus_tag="ACHAUG_001710"
     CDS             23843..24289
                     /locus_tag="ACHAUG_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8480802.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4353 c         5186 g         7090 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaatcataac
    15601 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    15661 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    15721 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    15781 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    15841 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    15901 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    15961 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    16021 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    16081 caaagacgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    16141 cgggatattc aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa
    16201 gcgtttatgt ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga
    16261 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    16321 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    16381 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    16441 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    16501 catcccgttt ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg
    16561 ctattactat cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    16621 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    16681 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    16741 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    16801 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    16861 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    16921 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    16981 ttatggaaca gctttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc
    17041 aaaacggatc atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    17101 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    17161 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    17221 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    17281 gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    17341 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    17401 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    17461 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    17521 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    17581 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    17641 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    17701 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    17761 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    17821 catcatgagt tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag
    17881 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    17941 gatgtttggg cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca
    18001 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa
    18061 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    18121 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    18181 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    18241 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    18301 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    18361 gattatcgaa tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta
    18421 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    18481 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat
    18541 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    18601 aatggagaaa atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc
    18661 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    18721 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    18781 gtaatcgtta ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca
    18841 aacttatccg tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca
    18901 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    18961 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    19021 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    19081 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    19141 ggacgtggcg tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta
    19201 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    19261 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    19321 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    19381 ggaaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt
    19441 gatttggcag cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt
    19501 agagaagctg ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg
    19561 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    19621 actgctgttg gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta
    19681 gaagaaacaa ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact
    19741 cttttttttg cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga
    19801 taaaagaatc ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag
    19861 tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg
    19921 aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat
    19981 taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat
    20041 tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata
    20101 cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa
    20161 aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg
    20221 aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc
    20281 caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa
    20341 ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac
    20401 aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt
    20461 taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg
    20521 gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat
    20581 tggtggaaac aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa
    20641 aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag
    20701 aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact
    20761 ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa
    20821 cggatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag
    20881 tattgacagg aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacaccaat
    20941 cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc
    21001 ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat
    21061 tcattgctga aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat
    21121 tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag
    21181 cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg
    21241 acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga
    21301 caatgagcgg acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag
    21361 aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga
    21421 cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga
    21481 agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa
    21541 tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg
    21601 cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa
    21661 aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca
    21721 cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc
    21781 gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag
    21841 cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg
    21901 tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac
    21961 tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag
    22021 agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa
    22081 aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga
    22141 tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg
    22201 acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct
    22261 cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt
    22321 tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga
    22381 tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc
    22441 tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct
    22501 agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca
    22561 gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca
    22621 ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc
    22681 acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct
    22741 tgattgatca atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc
    22801 atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga
    22861 tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg
    22921 acaaaaaaag taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt
    22981 tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag
    23041 gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac
    23101 ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc
    23161 tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac
    23221 gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat
    23281 ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat
    23341 cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat
    23401 ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga
    23461 tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca
    23521 agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt
    23581 tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca
    23641 tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac
    23701 gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac
    23761 caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct
    23821 gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac
    23881 agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat
    23941 acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt
    24001 gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga
    24061 cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca
    24121 actttccgac gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc
    24181 atcaatgaaa gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc
    24241 agatggaaac caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt
    24301 aaaggctctt cgtcaatgag tgttggtgaa gaaattc
//
LOCUS       DAYYBH010000039        23645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000039 DAYYBH010000000
VERSION     DAYYBH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.039"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHAUG_001711"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHAUG_001711"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8480803.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACHAUG_001712"
     CDS             complement(1174..1869)
                     /locus_tag="ACHAUG_001712"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8480804.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACHAUG_001713"
     CDS             complement(1882..2598)
                     /locus_tag="ACHAUG_001713"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8480805.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACHAUG_001714"
     CDS             2928..3536
                     /locus_tag="ACHAUG_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8480806.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACHAUG_001715"
     CDS             3861..4184
                     /locus_tag="ACHAUG_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8480807.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACHAUG_001716"
     CDS             4366..5736
                     /locus_tag="ACHAUG_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8480808.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACHAUG_001717"
     CDS             complement(5866..7167)
                     /locus_tag="ACHAUG_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8480809.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACHAUG_001718"
     CDS             complement(7521..9512)
                     /locus_tag="ACHAUG_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8480810.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACHAUG_001719"
     CDS             complement(9656..9922)
                     /locus_tag="ACHAUG_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8480811.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHAUG_001720"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHAUG_001720"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8480812.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHAUG_001721"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHAUG_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8480813.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACHAUG_001722"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACHAUG_001722"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8480814.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACHAUG_001723"
     CDS             complement(14082..14603)
                     /locus_tag="ACHAUG_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8480815.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACHAUG_001724"
     CDS             complement(14606..15448)
                     /locus_tag="ACHAUG_001724"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8480816.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACHAUG_001725"
     CDS             complement(15441..16166)
                     /locus_tag="ACHAUG_001725"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8480817.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACHAUG_001726"
     CDS             16342..16638
                     /locus_tag="ACHAUG_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480818.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACHAUG_001727"
     CDS             17206..18798
                     /locus_tag="ACHAUG_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8480819.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACHAUG_001728"
     CDS             18811..19551
                     /locus_tag="ACHAUG_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8480820.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="ACHAUG_001729"
     CDS             19719..21068
                     /locus_tag="ACHAUG_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8480821.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="ACHAUG_001730"
     CDS             complement(21196..21786)
                     /locus_tag="ACHAUG_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8480822.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21933..23285
                     /gene="brnQ"
                     /locus_tag="ACHAUG_001731"
     CDS             21933..23285
                     /gene="brnQ"
                     /locus_tag="ACHAUG_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8480823.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6771 a         4761 c         4503 g         7610 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtatctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caatcaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacaaactt ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcca tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgaacaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaacagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatta ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact cttttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagtt aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccgtcg ttttatatag gaaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact
    21961 atctttttat aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc
    22021 ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg
    22081 ttaccggtat cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag
    22141 gtgtatatga attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc
    22201 tttacttagt tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga
    22261 ttggtttagc accatttatc gatcctagca gacagacgct atatctagca attttcagta
    22321 ttgtattttt tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg
    22381 ttggaaagtt cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt
    22441 ttttgaatcc actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg
    22501 cttttttcac agggtttact caaggatata acacattaga tgcgcttgct gctttagcat
    22561 ttgggatcat cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg
    22621 ctaaagacac gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt
    22681 tgctcgctta tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga
    22741 tcacgctggc tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat
    22801 ttatcgttat attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa
    22861 cattcacaga tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc
    22921 ttccttgttt agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt
    22981 tgatgttcat ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt
    23041 ttaagcagcg tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt
    23101 ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt
    23161 tatatgttgc ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag
    23221 cagttatcgg ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag
    23281 attgataaaa gaggttgtga aagagacgtt cagccttatt gccgataggc tgtctcttga
    23341 acactgttta ttgagaggtc gtgaaaaagc cgctcagttt cgagtattaa gaaatatttt
    23401 ttgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt
    23461 gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa
    23521 gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac
    23581 aacatttctt ttataatata gattttagac atatatatga tataaaaata gttttcgcaa
    23641 aaaaa
//
LOCUS       DAYYBH010000040        23547 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000040 DAYYBH010000000
VERSION     DAYYBH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.040"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1134)
                     /locus_tag="ACHAUG_001732"
     CDS             complement(<1..1134)
                     /locus_tag="ACHAUG_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFM8480824.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVN"
     gene            complement(1225..1677)
                     /locus_tag="ACHAUG_001733"
     CDS             complement(1225..1677)
                     /locus_tag="ACHAUG_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8480825.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(1690..2100)
                     /locus_tag="ACHAUG_001734"
     CDS             complement(1690..2100)
                     /locus_tag="ACHAUG_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8480826.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            2375..2587
                     /locus_tag="ACHAUG_001735"
     CDS             2375..2587
                     /locus_tag="ACHAUG_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8480827.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            2590..2802
                     /locus_tag="ACHAUG_001736"
     CDS             2590..2802
                     /locus_tag="ACHAUG_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480828.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2942..3097
                     /locus_tag="ACHAUG_001737"
     CDS             2942..3097
                     /locus_tag="ACHAUG_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480829.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(3094..3318)
                     /locus_tag="ACHAUG_001738"
     CDS             complement(3094..3318)
                     /locus_tag="ACHAUG_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8480830.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3396..3584
                     /locus_tag="ACHAUG_001739"
     CDS             3396..3584
                     /locus_tag="ACHAUG_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480831.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            3581..3754
                     /locus_tag="ACHAUG_001740"
     CDS             3581..3754
                     /locus_tag="ACHAUG_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480832.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            3723..3851
                     /locus_tag="ACHAUG_001741"
     CDS             3723..3851
                     /locus_tag="ACHAUG_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480833.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            3910..4245
                     /locus_tag="ACHAUG_001742"
     CDS             3910..4245
                     /locus_tag="ACHAUG_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480834.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            4478..5419
                     /locus_tag="ACHAUG_001743"
     CDS             4478..5419
                     /locus_tag="ACHAUG_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8480835.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            5421..6311
                     /locus_tag="ACHAUG_001744"
     CDS             5421..6311
                     /locus_tag="ACHAUG_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8480836.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6354..7154
                     /locus_tag="ACHAUG_001745"
     CDS             6354..7154
                     /locus_tag="ACHAUG_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8480837.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7169..8020
                     /locus_tag="ACHAUG_001746"
     CDS             7169..8020
                     /locus_tag="ACHAUG_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8480838.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8221..8682
                     /locus_tag="ACHAUG_001747"
     CDS             8221..8682
                     /locus_tag="ACHAUG_001747"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8480839.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            8708..9160
                     /locus_tag="ACHAUG_001748"
     CDS             8708..9160
                     /locus_tag="ACHAUG_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480840.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            9266..9460
                     /locus_tag="ACHAUG_001749"
     CDS             9266..9460
                     /locus_tag="ACHAUG_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480841.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            9491..9784
                     /locus_tag="ACHAUG_001750"
     CDS             9491..9784
                     /locus_tag="ACHAUG_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480842.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            9715..10254
                     /locus_tag="ACHAUG_001751"
     CDS             9715..10254
                     /locus_tag="ACHAUG_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8480843.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            10329..10796
                     /locus_tag="ACHAUG_001752"
     CDS             10329..10796
                     /locus_tag="ACHAUG_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFM8480844.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            11543..11773
                     /locus_tag="ACHAUG_001753"
     CDS             11543..11773
                     /locus_tag="ACHAUG_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480845.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            11932..12765
                     /gene="terS"
                     /locus_tag="ACHAUG_001754"
     CDS             11932..12765
                     /gene="terS"
                     /locus_tag="ACHAUG_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8480846.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            12758..14173
                     /locus_tag="ACHAUG_001755"
     CDS             12758..14173
                     /locus_tag="ACHAUG_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8480847.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14185..15714
                     /locus_tag="ACHAUG_001756"
     CDS             14185..15714
                     /locus_tag="ACHAUG_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8480848.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            15632..16684
                     /locus_tag="ACHAUG_001757"
     CDS             15632..16684
                     /locus_tag="ACHAUG_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8480849.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            16686..17003
                     /locus_tag="ACHAUG_001758"
     CDS             16686..17003
                     /locus_tag="ACHAUG_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480850.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17130..17753
                     /locus_tag="ACHAUG_001759"
     CDS             17130..17753
                     /locus_tag="ACHAUG_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8480851.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            17768..18658
                     /locus_tag="ACHAUG_001760"
     CDS             17768..18658
                     /locus_tag="ACHAUG_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8480852.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            18681..18896
                     /locus_tag="ACHAUG_001761"
     CDS             18681..18896
                     /locus_tag="ACHAUG_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480853.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18908..19249
                     /locus_tag="ACHAUG_001762"
     CDS             18908..19249
                     /locus_tag="ACHAUG_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480854.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            19249..19620
                     /locus_tag="ACHAUG_001763"
     CDS             19249..19620
                     /locus_tag="ACHAUG_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480855.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            19613..20008
                     /locus_tag="ACHAUG_001764"
     CDS             19613..20008
                     /locus_tag="ACHAUG_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8480856.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20010..20387
                     /locus_tag="ACHAUG_001765"
     CDS             20010..20387
                     /locus_tag="ACHAUG_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8480857.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            20388..20996
                     /locus_tag="ACHAUG_001766"
     CDS             20388..20996
                     /locus_tag="ACHAUG_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8480858.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20996..21337
                     /locus_tag="ACHAUG_001767"
     CDS             20996..21337
                     /locus_tag="ACHAUG_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480859.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            21579..>23547
                     /locus_tag="ACHAUG_001768"
     CDS             21579..>23547
                     /locus_tag="ACHAUG_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8480860.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFIN"
BASE COUNT         8385 a         3467 c         4725 g         6970 t
ORIGIN      
        1 attaacttct tgttttctaa aatgattatg caatttagtt tccaaagacg gagcgtcctc
       61 tgaaaaaatc atcgcatgaa cgtcaaattc aaatggaacc gatgcactac tcaattcttt
      121 tattctatcc ataggttcta atcttctagt cataccaatt ttatatatgt cttcaccaaa
      181 agacccaata ttactaatta catacacata accggctcta gtatttaatt ctctttgtaa
      241 tacattttca cgtataactt ctaattcttt taatttttct tccagttctt gaattttatt
      301 tgcatataat tctttttcca catctgcatt agctttttgc atatatgcga ttaatttgga
      361 aatttcagaa ttgaattgac gttgttcttt atcaatacgt ttcttttcac gatcaatttc
      421 tctacgaact ttttcctctt ctatcatctg ttcttttatt gctttttgtt gttctttttc
      481 ttcggtaatt ttcactaaat aagagtaatg aagttctagt tcttgaagtt taaactctaa
      541 aagacccttc cttaattcag cattgtcatt cttgaataaa ttattaattt tatcaaatat
      601 ttttatgatt ttttccctag tcgagtctat attagaaata gttagtttat tgagtaatcc
      661 tttcacttct gaatcaaatg gaatgagcat ttttttcttt aaattatttt tctctcgttt
      721 atttaaaata agagggacag aaacagcatc gtcaataata tattcctttt cttttaattt
      781 taacaaagaa agcttatttt ttatttcaga agatgaagtt tcttctatat ttgaaacgat
      841 aagtgaagaa tagacatttt ttaatgtttc atcatacttt tgatttattt catcatttaa
      901 atcttctttc tcttttttta ataaaataat atcatttttt aacatagtaa tttgtttatt
      961 taaatctgat agtgtttctt ccttacttaa tataaatctt tctctatcat gtaatttatt
     1021 tagtttttca tcattttttt gttctaattc ataagcttct ttttttattg tgttagcttt
     1081 cgacagttta gtgattccta taattaataa aataagtcca atcaataata acattcaaaa
     1141 ctcctcattt ctttgatata attattgtag agtttactca gaaatgagga aagagtccat
     1201 gtgaaagcat gggctttttt atttttaatg ttgtaatact aaatgttcaa gattatgagg
     1261 aagccctaaa tattcaataa tttggaactg actcatatca gataaatctt gttcatctat
     1321 tgttctagtt aacatttcaa ttgcaaacat attagcttca gcttctatac cataaatcat
     1381 acaactaagg ttgtttgctc taaaagctgc tgtgcaaata cctttatgga gtctaacgtg
     1441 ggctatttca tgccataata caaatctttt catagaaggt gataggtttt gattaattaa
     1501 tatcacataa catcttttat tgtgtatcgt ttgtcctaat gttttattcc ctaaatcagt
     1561 ttcaattaaa tctatatcag aatatttgat tattttatat ggattaatcg catcagatcc
     1621 aaactctaca aataattttt ctatttcttc taatatccag tatttgttta aatgcatacc
     1681 aaagcccacc tattgacgat attttttagg agtgaatttt ttctttgctt taactttggt
     1741 taaggcaagt gcaagttgaa tggcatcgta taatttttcc ttatcttcat cgtctaaagg
     1801 ttcaccataa taatttgttt cagcatctga attcaagcct tcaagtaatt tttcagcttg
     1861 taaagcaata tcttgtttat ctttttctgt taaatcataa taatggcgtt tatcagtacg
     1921 tcctaaaaga taatcagttg atacattaaa gtaatctgca actttttgta ttttatctcc
     1981 agatggagta cttttattcc atctagacat gcttccattt ccgaaattta actttctttc
     2041 aagttctgcc aacgtcattg aacgttcttt taataagatt tttatacggt ctaatagcat
     2101 aggatatgca ttcctttcaa aataccgaca aaaaaagtat ttttctgcaa attatctatt
     2161 tacaaataga gaatttgcag ttatactagt tttgtaagca agatatttac ggcatacaaa
     2221 gactaaaagc aataaactaa tagtctcccc agacatcagt aagctcgctt tttgtgcgct
     2281 gtattttgta tgcttacata atagcatatt tgcagttttt gtcaaactta ttagacaaaa
     2341 atattagaaa atattcgata agggggtatg aagcatgact ttaaaacagc aagtattaat
     2401 tgctttagta aagaaaggtt ggagtcaaag agagttagct cgacgaatgg gtatatcaat
     2461 tacttattta cgagatatct tcatcgaaaa aagaaaacct aaggaacgtt taaaacaaat
     2521 cgaagaactt ttggatatca agttagaagt agattctaat gatcagccat cagaacagga
     2581 ggcgtaaaaa tggtacaacg tttgagcaaa gcggagttta aagagaaata taagtattct
     2641 gaaagtactt atcaaagaag aatgaaggaa tttaaaaaat cacgttttag tgaggggtat
     2701 aacgcagtta cttccaaaga aataacaatt gatgtcgatt tatatgaaca gtttagaaca
     2761 tggcgttcta aaaatcgatt tagagttaga gctgtttgtt aatagctaaa aaatctaaat
     2821 tcaccacttc tgaaaagagg agttgctgct ctcctttcaa gtttgccaaa gtccacgttt
     2881 tttcattttg gcaactcctc ttcttggagg tggtgacggc gagagacgaa ggagggatga
     2941 catgaaggaa gaatcaaaaa tagggatttt gaatgagaac aattacacaa gattaattat
     3001 tgaaacagaa gaagggaaaa aagtagcaga agttacattg accgatgcaa ctcctgctga
     3061 cggttatcgt attcgattaa ctcctaaata tgattaacct tttggtgggt gtggatcgtt
     3121 tccgtgacta tcacggcttt gaatttttcc atctctgcca tgaataaata gctcagatcc
     3181 ttgattttta gaaatctctc tagcgatttt tactgcttct gattttgtat tagtatgtgc
     3241 agtcgcttta gaatttccag cacctttgac attccaacca ccattggaat taggtacaac
     3301 atgttgattt tttcccatag ttttcacctc catttctata gaaatatttt atcaaataga
     3361 aatggagaaa ttcaataaac gaaaggagaa gtgatatggc atataccttg caacaagaac
     3421 atcaaattct cggtttgatt aaacaacgca ggaaacaatt acaagatgac cgtgcagcgc
     3481 ttagaaaagc cgatgagcta tcagataggc aagctgaact aattgcttct gaacttgagg
     3541 atttgagaat gctagaaata aaaaataggg agattagatt atgaagaaga cagacacact
     3601 ttttatagga ttcattttgg ggttattagt gattgtagcg caccaaagta ttatcggggg
     3661 aagcttgttc gcagcattga tggttttaat caatctgctt gattcaaaag aaaggagcaa
     3721 ctatggcacg agaagaagcg ctaaaaatcg gtaaagtgat tgctgataat cggtggggaa
     3781 atagccgccc tattatttta agcaagcaac atatcgaaaa gcaaaaagca tggcaacaaa
     3841 taaaaaagtg actctgccag ccagcaaaga gtcacataca aatttataaa actaggagaa
     3901 ttatatcaca tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa
     3961 aagcaaggtg tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt
     4021 tatggtgaga caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa
     4081 aatctcccat tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg
     4141 aaaaatgaag aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat
     4201 ttagctgatg tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa
     4261 ttgatctact cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct
     4321 ttggcttgtt attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg
     4381 ttaaacatga acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa
     4441 ccaccatatg aagcaccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat
     4501 gagccatcaa gaatggcttg aagaccgtaa gaaaggcatc ggaggttcgg atgttggaac
     4561 aattttagga ttgaataaat ggaaatctcc ttatcaacta tggctagaaa aaacaggaca
     4621 agtagtactt gaagaatcag gaagtgagcc cgcttattgg ggtaatgttt tagaagaagt
     4681 ggttgctaaa gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt
     4741 tgaacatcca ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa
     4801 tgccattctg gaatgcaaaa cagccaatca atttctcggt aaagaatggg aaggcgaaga
     4861 ggtaccgctt agctatctct gtcaagttca acattacatg aacgttctaa acaaagacta
     4921 ttgttacatc gctgtcttaa tcggtggtca aaaatttatc tggaagcgga ttgaacgaga
     4981 tcaagagctg atcgatacaa tcactgaaca attagtagag ttttgggaaa caaacgttct
     5041 tggaggtatc gagccaatta ttgatggtag tcaagcgaca gctgatttct tgaaagagaa
     5101 gtatgcagat gttgaagacg ttcaaacgac gttaccaatt catttcgatg aactagttga
     5161 acagaaaaac gaactcaaac ggactaagaa agaaattgaa tcagctatcc gtcaagtaga
     5221 caacgagatt atcagcgagc taggtaaacg tgaagccagt atcggtatca ctcaaaaaaa
     5281 catcatcagt tggaaatttg tccgtacgag acgtatgaac tcgaagaaac tagcagagaa
     5341 atatccagat gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac
     5401 cgaaagggag atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagcaa
     5461 agccacagaa acaagttgca ccaggtcaat taggacttaa agctttgatg aatacaccaa
     5521 caatgagaaa gaagttcgaa gaggtgcttc atgacaatgc taatgctttt atgtcgaatg
     5581 ttatgactct tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga
     5641 gtggtgcgtt aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat
     5701 atttagttcc attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat
     5761 tcatgcttgg ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat
     5821 taaatgtgat cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt
     5881 ttgagtttga tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg
     5941 agttactgaa tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc
     6001 ggattgctaa caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt
     6061 acaatgcaat ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt
     6121 ccatcgaaat gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg
     6181 gcagcattat tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg
     6241 ttatatctga agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt
     6301 taaacaaata atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca
     6361 caggatatgt caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata
     6421 tgtataaatt atggaacttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt
     6481 ttaatggaaa agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca
     6541 catctgagat gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga
     6601 gatggctaaa acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca
     6661 gcgttatatc aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca
     6721 aacgcacaac aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg
     6781 aaaagaataa taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact
     6841 cagtttacta cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca
     6901 aaaagccgaa tctgcaaagt tgggcagatg acattcggaa aatgatcgag atcgacaagc
     6961 ggaccgaaaa ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat
     7021 cgaatatttt atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa
     7081 atcgtgatta taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg
     7141 agttgcctat ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct
     7201 gatacagaaa gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg
     7261 atggcgtacg aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg
     7321 gtataaagca ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct
     7381 gaaagcaaga gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt
     7441 tgattgcaca ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt
     7501 aaccaaacgc tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg
     7561 aaaacaagga acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg
     7621 atcaaactac gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa
     7681 attttcgtac aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat
     7741 caaaacagcg gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc
     7801 agatagtcga gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct
     7861 agcaacgatt atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag
     7921 aacaatatca agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc
     7981 agacaagcgc ataaaaccag tcaaaggagc gatcgcatga atttgactaa acaagaaaaa
     8041 agaatctgtc gtcagataat gttgcaaatt ataggtgatc gtgacatgga cgaattatct
     8101 caacaagaga gtcgaatggt tcggaagata agtaagctaa ttatttatcg ggatacagaa
     8161 agatttctca ctgttttagg taacgtctac gagaatccag agttattgga ggtagagtaa
     8221 ttgccaaaaa tattagatgc atgttgcggt agtagattgt tctggtttga taagaacaat
     8281 tctgacgtaa catttatgga ttgtcgtcag cagtatgaag aattatcaac aggtcatgtg
     8341 atcaatgttg atccagatgt ggtcgcagac tttcgcgata tgccttttga agatgatgtg
     8401 ttcgatatgg ttgtatttga cccgccacac ttaagacatg tcggcgaaaa ctcatggctt
     8461 gccaaaaaat atggaaaatt agacgaactt tggccagaag acatcaggca aggttttgca
     8521 gagtgtatgc gagttttacg accttcagga acattaattt ttaagtggaa cgaggaacag
     8581 ataccacttt ctgatgtgtt agaggctatt ggagaacagc cattgtttgg taataaacga
     8641 agcaaaacac attggcttgt gtttatgaaa aaattcagct aacggaggaa acaaaggagg
     8701 aaaagtaatg aagattgtat taaataaatg ttttggtggc tttgggttga gccatgaagc
     8761 aaaaatggaa atttttaaaa gaaaaaatat tgaagtattc ccatacatca ataactttag
     8821 ctatgactca gatgatgaat acacgagata cacaggccaa aagctaggtt cgatggattt
     8881 tatctactat ttcaaaaaag acccgaaaat cgacaaagtt acaggaattt atagcgaaat
     8941 tgaccgactg tacggaattg ctgatgattc cagtttttca agtgattcaa acagaggtga
     9001 taaggacctc gttgctgttg tcgaaaaact aggaagtgag gccagtggac catatgcaag
     9061 tcttaaagtc gttgatatac ccgatggggc tgagtgggaa ataagtgatt atgacggcgt
     9121 tgaaactgca cattatggtt ttcaaacagg tagtatttag tccactaatg acaactaaag
     9181 aggaggcaga aggatgagtg aagatgaata ctttgaacag tagtctatta acgacagaga
     9241 tttttaactg gaaaggggtg tagtcatgga cgcatcggaa gtagtaaata aaattaatag
     9301 tctcaaagag gttttcggag acgtcgaagt agtgataaac gcacaaggct atgacaacga
     9361 attttttaag cagattaccg acattagcat tcagcaagga atggaagacg aagacggaaa
     9421 ctttatcgat gaagtagcca ttttagtgac ttgcgagtaa gtaattgaca gctaaagagg
     9481 aggaagcgga atgaaactaa aagacggatt ttactccagc ggccacggca tcggcggttt
     9541 aatgctagat atgccgacaa agaatcctaa aacacgtaag aaaccaaaat tcaaagtcgg
     9601 tgacatggtt cgctgcgaag cagaaggatt catctatcca tttcgtggat atgtagaaca
     9661 cgtctataat cactcagcaa tcattcgcat tgaaaacacg atggaatgtg ataagtggtt
     9721 agcgaaaagc aaagagaatt tagcagtggt gagattggtg gacatggaag ttataaaatc
     9781 ttaattttgc ttttttacaa ctatcctaca acaatgagcg ttattccaca actggaagga
     9841 gaagaggcaa ttgtgaacat tttagagaat atagatatta agcaaacaag gaaaaatgct
     9901 agacgattac taaaaagata cagaaattta gaacgtttgg tcggaccagt gaaaatagac
     9961 ttttccgtga tgactgttac caaaaatttg aaattcacaa ttgacagtca aaacgaagaa
    10021 atcattgaag cgataagtac tcgagattcg gttatcgaag cattaacacg gctaagtaga
    10081 atccatttcc aggtacttta ttatagttat tgttttccta ataagatgtc aatgtatcaa
    10141 ataggagaaa aattaggata ttccgataga actatcgaga gaatgaaggc agtagctttg
    10201 gttgaatttg ctgaggcgta taaatcagga gaactcattt cacgtacaaa ataaaaaaag
    10261 tcggattcct ccgactatga ataatatttc cgacataagt attatatcat aattggggga
    10321 atcagaggat ggtacttttc gatgtaaaga aatatgagac accaagcgcg aaagatgttg
    10381 atatggaacg cacaaaacat aatgtcgctg tgtttctttc agcatatcta tcagctagat
    10441 gtagagtagg gcaacatcga gaaccaaaag tgacagcatc ttattccttg gttccacctt
    10501 ctacagctga tcatatattt gaagcagaaa gaatgctgat cgataaagaa gaagcacagg
    10561 aagaatttga gtatctgcat aaattgtttg ttcgaggcta ttcagcgata cagcatccgc
    10621 ataagcctga tgtgactgaa aggcgcaaga agatattcta tgatcgttat atcaatggtc
    10681 tgcccattta tgtaactgct caaaggaata atactagcga agaatcggtt aaagtagaat
    10741 caaacagaat tatcatccaa tttgcttcat cgttagaact ggttgctttc aagtagccag
    10801 tttttacact ttttataccc ttttattacc aatttggttt ccattttata ccttttttat
    10861 accaatcact tacctattca acgttgtatt atgatagtgt cgaaaaatta ggaaacagga
    10921 tcgacaaaat aaaatgtaag ggaggaaatc tccctcatcg tttaattaag cttcgatagg
    10981 cagcaacgga aatattaaga ataaggatgt gaatttcaac tccttctaaa ttgttcttat
    11041 tatctatcat ccgttgctgt ctattgtttt tttaattatt cacacgataa ctaaaggtaa
    11101 gtgaagagaa atgattccat taataatttc aatttttgcg ctttgtctta atgtatatat
    11161 gattggattt aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg
    11221 tcactgtggc ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca
    11281 tgcaaggttc gattccttgc cagtgacttt ggcaaccgag gcatcggcgg tttaaaaata
    11341 taggggtgcg caatttcgta cgcgttttgt gcatcgtgta ggttgctatt acataactgg
    11401 ttaggttaga ttgagatttg ggattcggta caaatgaatc gtcaaatgtc tcaagcacag
    11461 gatcggaaac gtccctgcct gtgcattaca tattagatca ctctttgagt ggtctttttc
    11521 tttacataaa ggaggctaca taatgagaaa ctactggtat gtatcgctaa ctaatgaata
    11581 tcctcgaacc attgatgatt gttcagtgcg tgttgtgcgt tctgtacaaa tcaaagggaa
    11641 gtactctatt gtcgaaatgg ggagagaagc agaaccatgt gagattgatg cgtgcaagct
    11701 cgtttattgc ggtcatggtt tctatgatga accaaacatt caaaataata ttaacaagaa
    11761 tttgagggat tagaatgcaa gaaatggctt aaaaatctcc aatcaggaca aatcaagtta
    11821 gacaaagttt ccgatttaaa gatattgatt gaagctgatt taatgctaaa agatattgaa
    11881 aattagaaaa caaaactcaa cctaaacaga atgtgaggtg gtgtgtattg aatggcaaga
    11941 caacgtgatc caagacgtga tgaagccaaa agaatttggt tagaatccaa cggagaaaag
    12001 cagttaaagg aaattgcatc tgaattaaat gtttcagatt ctcaggttag aaaatggaaa
    12061 tcgcaagaca aatggagcgc tgaattgatt agtaacgtta ccaatggcaa aagtaacgtt
    12121 actaatcaag gtggcgctcc tattggtaat caaaatgcta aaggtaacaa aggaaatagc
    12181 cgagcttctc cgccagtggg taataaaaac gctttgaaaa caggtgagta tgaaacaata
    12241 ttttttgaca cgttaaccga taatgagaag gacatctatt ctagtttgaa tgatgatcct
    12301 tcttttgctt tgtccgaaga aatacgactt cttaaaataa gacaacttcg aatgatgaaa
    12361 agaatccaac aagctgaagc tggactaaat gatgaagaag tcgaacgatt gcagcagcta
    12421 agaaagatta aaactccgat tgaaaaaaat ggtaaaaagc tagaaatcaa gcgtgaggtt
    12481 atgcaagatg tgcagattag cagaaaaaaa catcgcaaaa ttgatgatat tctttcaatt
    12541 gaagattcat tgactcggat tagcaaccag ttagctaaag ccatcaagca aatgaatgaa
    12601 ctttatatga atgaatacag aactgattta attaaagctc agactgataa gatccaagct
    12661 gagacaaatg aaattggcgg aaataattca ggtgaagaaa tagaagaatg gaaacaggca
    12721 gttttaaatg ccgcaaacaa acgggcggtg aaagaaaatg aataatgaat ttattccttt
    12781 tgctgatatt ggttctgcca ttgattatta ctatgataaa ccagtagctt tttgccaaga
    12841 tattttgcat ttgaatcctg atgaatggca agaaaatgtt ttaaatgatt tagctgaatt
    12901 ttcaaaggtt tctgttcgtt ctggtcaagg agttggaaaa acagcattag aagcaggagc
    12961 aatactttgg ttcttaacgt gtcgacccta cgctaaagta atagcaacag ctccgacaat
    13021 gaagcaactt tacgatgtac tttgggcaga ggtagctaaa tggttaaatg atagcttgat
    13081 caaaaactta ctgaagtgga caaagaccaa aatttatatg gttggtgatt cagagcgttg
    13141 gtttgctacg gctagaacag cgactaaacc agaaagtatg caaggttttc acgaggacca
    13201 tatgttgatt gtggtagatg aagcttctgg tgtgtctgat ccaattatgg aagctattct
    13261 tggtacgcta tcaggttttg ataataagct gttgatgtgt ggaaacccca ataatattga
    13321 aggtgtcttt tatgattccc ataattcaga ccgtgataaa tacagagttc ataaagtatc
    13381 aagctatgat agtaaacgta caaacaaaga caatatagaa atgattctta aaaaatatgg
    13441 aaaagaaagt gatgttgctc gtgtccgtat ttttggagaa tttccgaaag gtgcgttgga
    13501 ttcatttatc agtcttgaaa cagttgaatt ggctacagaa aaacaaatta gtgattcttt
    13561 agtcaataaa acaaaggttg ctcatattgg tgtcgacgta gctcgatatg gtgatgattc
    13621 tacgattctc tttcctagaa ttgctaccag ggcattggag tatgagaagt attcaaaacg
    13681 tagcaccatg gaaacaacag gatatgtcat caacatggcc aagaatctaa tgagtcaata
    13741 tccgagtatt gataaagtga tgattaaagt cgatgacact ggtgtcggag gtggtgtaac
    13801 cgaccgccta gaagaactta tagaagacaa acattatcct tttgaggtgt ttggagtgaa
    13861 taacggttca acatcagaag acgattttta cgataattta ggtactcaac tatggggaaa
    13921 catcaaggaa atgttagaag aaaatatgac agcaaatctt aacggagaac agcctgttat
    13981 tgaattgcct tctgatagtt cgttaatcaa agaattaagt actcgcaaat tcaaaatgac
    14041 aagtagaagt cgtatacgtt tagaaagtaa agatgatatg aaaaagcgaa atattggtag
    14101 tcccgatatt gctgacgcac tggctttagc gttttatgag ccaccaagtc actatcaatt
    14161 tattcaattt taggaggtga gcttttgatt agtacagtat tatctttaga gagatataag
    14221 aagctacgtg ttaaatatgc aacacaaatt gaagatggaa tgtttgatcc gaatggtttt
    14281 atagaagata tgaaaccatt ttttgctgat cgcgaaagaa aatatctagc ttatactagc
    14341 gaaaagaatg aaatagataa tagaccaaag cctaacactg atattgtaaa agttaataat
    14401 aaacttcatg ctggtatgta ttcaattgtc gtagaccaag cagtcaatca ttttactggt
    14461 atacctatca aatgggatta cgatgtatcg gaacaaaaaa gaacactcat tcaaagatta
    14521 aaagataaat tcttaaaaaa tgatatagaa cttccgacag ttccagaagc ttttaacaaa
    14581 ttaacaagca atcttgattc catgagattc gcaatgcttg attctgaaac tgcaactttt
    14641 caaggagctt gtggagtagc tttccgattg ctagagcctg tggaagaaga tgatggttgg
    14701 aagttaagag caagtaatat tgaaccttgg agagcagaaa gatatggaaa tgccggaatc
    14761 tatatcaaag aaaagtatga ctcttaccag aaaaaatttt ttcaagaaat gaaagttatg
    14821 acaagaaata aaattctgac atatgcttgc tatggtgatt tgaattttgt tacaagcgga
    14881 acgttcaaaa aaattgatga gaccgataac cctttgggga cgattacctt ggcagaattt
    14941 aaaaataata cgaatcgtta ttgtgatttt gaagtagctg aggaaattgg agatgcaatt
    15001 gatcgtgctt tgtctgatca acaaaacgaa atcgaacaat ttaagcttgc gtacatgctt
    15061 gttactggca caacaatgag caaaggcact gctaaagaaa tgatgaatca gctagggatt
    15121 atcaacttaa aagcccctac tgcaaaagct gaatatgtca caaaaaattt agcaaaagat
    15181 ttcaatgaat atcatatgga tctattgaaa aaacagtttt acaccatttg taaagcaatc
    15241 gattttaacg atgaggtgtt taaatcaaac agttctggag aagctcgcaa gtggcaaatc
    15301 attagcctag aagcgaaaac aaatactaaa gagcaatatt ttagagaagg attgaaagaa
    15361 tgcgcagaaa caattgctgc ttttcttaag tttcatgaca aagtagaaat tgagcctgaa
    15421 aaaattattt ttactttctc tagatctttg ccaactgatt taagttattt agctgaagct
    15481 ttacctaaac ttgcaccata cgtatccaaa cgaactattc agagccaaat tccatttgta
    15541 acagatgttg attatgaaaa tgaaatgatg gaattggaaa gtggaagtgc ttatccagat
    15601 agtgaataca attttggcgg aggtggcaat agtgacgata gaaacgaagt attggaccaa
    15661 acgtcgagaa ctggaggatc aagcaaggct caaacaagaa aatcagacac ttaaaaaatt
    15721 aactagtgta tttcctgaag cactgaaaga gatacaagca aaactattat cacaagctga
    15781 cttacacaat atcacttatc cagaaatgat ggagttttac agtacaagta atcagaaaaa
    15841 atatcgtgaa tatgtggaaa aaaattataa gtcattaaaa atgtatgatg caaaatacaa
    15901 agagtttatc gatgaatttt ttccaccatt tgactatgca aaagtcaatc gcttattaca
    15961 aatacgatca gatgtattta aaattcttgc agaatatgcg atggatgcag atgtgaatca
    16021 atatttttct gatcgcttag aggaaattct tcaaagaaca tattcttcta atgctaatgt
    16081 ttttgttcaa cttttaaacg ttgatatacc aaattattta ccagaaaatg aaattaaaca
    16141 ttatttgaac tatccatggt caggaaagac attttcaaga agactttggg gaaatatttc
    16201 atcgcttgaa cagaaactct ctaatgctat tgtaaaaagt gttgctagtg gagaaggcgt
    16261 tatacacgca ttaaacacca tgagattaga ctcagaaatt tgtgacatgt ttaagttaga
    16321 agaatcaaag tataacaaag cgatagaaaa tctcgttcga acggagtatg caaaatttgc
    16381 acaagatggt attgaaaaat catatttaga aacaggtatt gaggaataca acgtattgac
    16441 tgcaaaagat gagagagttt gccggatttg tggaggaaag gcaagtaaga atccctataa
    16501 actgaaagat gctgtcatag gtgaaaatcg agcacctttc catagtcgtt gtagatgtac
    16561 ggatgttcct aatttaccaa aattaggaaa ggatattgat gaagaatatg accgtttatt
    16621 tggcgattta ttagatgagt ttgcacatga ttcttttgga attaatttga aacggaggaa
    16681 gtagaatgaa agattttttt gaagcagtac taacaattaa tgtaaatgct gatattgcag
    16741 aagcctacaa aacagctatt gagtctgaga accatcctaa tggcttgaga gaccattgga
    16801 atggcaatta tgcctacgtg gttattggcg atcaaactgt taattatcaa gataatactc
    16861 cagttgataa gaataccgtt aatttaacga ttcaattatt atctcattca ttaccaaatt
    16921 taaaagaaac agttgattgg tatgaaaaga tgggatgtat tgttgttaga actgactaca
    16981 aagaaggaaa gtctagtaat taggcttttt tattttgtcc gaaatgacgt taaactagcg
    17041 caatactggg cttggttgaa tggtggggcg caactattaa aactcaaagc aatgcggggc
    17101 gtgaaaacga atcgtggggc gaaaggagaa tgagcatgaa acacaaatca ttaatgccaa
    17161 tgaatttgca atactttgcc gaaggtgacg atcaaaagtt ttcttttgat gacttcaaat
    17221 cttttgtgga atccaatgaa gaagctcaaa aatttattca gtcacaatct caaagtgttg
    17281 ccgacaaaca attggaagct tggaaacaaa ataacttaga taaaattaaa caggatacca
    17341 tcaaggaata tgaggaatct aagaaaaaca agtcacctga gcaaattcaa ctggaaaaac
    17401 tacaagctga atttgaagca gaaaaagcgt tgcgtgtgac aagtgataat aaagcatttg
    17461 ttgcagaaca aattgctgga ttagaactag atggagagtt aaaagagtct atttctcaat
    17521 ttatgctaaa taatcttgtt agttcggata cagatttcac taagaatgct gttgaaggtt
    17581 ttacaagtgt tttgaatgca attaatgaga aacatgcaga tgcattaaaa gaactacaaa
    17641 tgaagtctgc atttggtgga actcaacaat cgaataacca ggttcagcag aacaatgaaa
    17701 cattaacaaa tccagaagaa caattaggac aaattcttca acaatttaac taggagagtg
    17761 aaaaattatg aaaaaaacat ctttaaataa tttagagtat ttggatattt caccagcgat
    17821 taatgctatg caagtaccaa atacaccttt tttaagctat ttacttggtg ctggaaaaac
    17881 agagccagca aactcgacag aaattaaatg gcgtgaatat gatatcaaca acgatgattc
    17941 ttctgaaaaa cttgagggcg gagaatatcc agatgctgaa tcaggtcgaa cttggtttaa
    18001 caactatact gaaattttta gaaaatcaac ctctgtatct ggtacattag atgctattaa
    18061 tgtgaatgga gtcggaaatg aattaactaa tcaagtagca ctacgtggta tggaaatgaa
    18121 aattgatttg aaccgaaaat tgattactgg tgtaaaagct gatgaaaatg gttctaaagg
    18181 tcgtcgaatg aatgggattt tgaacttgat caattcagca aataaggcag aaacagccac
    18241 tgcgggtgca gtaacaagaa aagatatcga tactttattt aaattgatgt atgaaaaagg
    18301 ttatatggga gaaaaactat gcttgatttc tccagatatg caggagttaa tgactgatga
    18361 gttagatgga aaatcaacaa aaattgttca gttcggggaa agagtaactt ttggattgca
    18421 aattggaaat atcgtgtcta attacggtac aggtattgct ctactagaac catcattgcc
    18481 aaaaggaaca attgccgcaa tcgatactaa ttatgtgaaa ctacgtccat tacgtgaatg
    18541 gagagcagaa gaacttgcaa aaacaacgga ttctagacgt atcggtcttg taggtgaata
    18601 ctctcttgaa tacaatgctt caaactctgg ggcaatttta aatttaaagt ctgaataaaa
    18661 gggagttagt actccctttt ttgataggag gaattacagt ggtaaaaaaa gatgaaacta
    18721 aaaaagatga agtcgtgaaa tatagagtag gtaaaactaa aaattttgtt gggtttgttc
    18781 atcctaaaac tcgtagattt atcacagcag attcaaataa cgaatttatc atttctatag
    18841 atgataaaga agcaattgca attttggaag atgcaattga tgttaatgaa atttaggaag
    18901 tgatctgatg gatgaatcgc taaaaacgga aatcattgag tctacaaaag aagattttcc
    18961 agatttgagt gaagaacgca taactaattt attagaaata attttgctag aaattgaatc
    19021 atacaacact tgtaaaaatg atatttcatg ggaaaagtta aaaagcgtga ttagtgaagt
    19081 gttgtataaa ataataaaaa atgaatcaga aaaaacagta tcttcagtta gacgtggcga
    19141 tacaacgatt agttatgctt caacaacaaa tgatgttagt gagttacttc taggctatgg
    19201 tgatttgata cgaagagtta ttggttgtgg aggattggag tttttttaat gaatgaagca
    19261 gatattttag aaactactta tgaagatagt tgtattattg aaagactgac ggacattgaa
    19321 gattctaata caaatattac tattcaagat tacaaaaaag tatatgataa tcctatttct
    19381 tgcgctcttt cacaaggtca aatcgatgga ctagcagtca tagaagatgg agagatggta
    19441 aatgtttcaa ctgacacata taaattattt gttcatccta agattaaact aaaaaaagga
    19501 gatcgaataa caataactca aaaggccagt ggcttaattt tttctctatt tgctactaag
    19561 cctttttact atcctagtca ttgtgaagtg aatttgatag gaagtgagaa aaatgggtaa
    19621 tctcaaatat gaatcaaatg cagaaaagat cattgagaat tttaaaaata tgactgtaat
    19681 tgctcaaaaa gaaggaatat ccttcgttaa cgattcaatg aataaagtcg ttagtttagc
    19741 taaaccatta actcctgtaa aatcaggtaa tttaaggcga ggatatcgtg tagtaaaagc
    19801 tagaaaacta tcaagcggtc gaattgttgg agaagttata aacaatgaac attattttaa
    19861 atatgtagaa gaaggtcata ggactaaaaa tggtggcttt gtcaaaggga aattcatgtt
    19921 aactcgtgca acgaatcttg caaatatgtc ttatattcct cgaagattta aacaaatggc
    19981 aataaaaatc gttaagaaag gaaagtaaca tgaaagatga aatcattgct gcaatcagta
    20041 gcaaattaaa agaaatctat cctgatggga caatatatct tgattcagtt atgcagtcaa
    20101 ctaaagattt ttatttcgtt ttatcagtaa tggaatctgg aactgaaaat gtaggaattg
    20161 atattcaaaa tgtttctttc ttaattgata ttgcattgat tgataataac cctaatagaa
    20221 atttaattaa tgaattagtc tcacgttgtg ggactttttt taatacgatt acaatcgatg
    20281 agcaaatatt atttccaaaa gcctatttac ctgatgaagc agatggtgtt caacatattc
    20341 gttttacatt agaatttcca caatatattg aatggagtga aagataaatg ggagaaaaaa
    20401 gaagtaaaac cggaattatt tctgtcgaaa aacctacttg gtttcctttg gaagatgaga
    20461 cagggaattt tccggtatat ggtactgcgt tgccaatggg aacagcagta agtatcaaac
    20521 caacagctaa ttatgaaaca acacaagatt atggggattc agtggttcaa gatcaattta
    20581 cagcttttgg tggtgcagaa gttgaactgg aagcaaatgg atatactcat aaagttttat
    20641 ctacaattac tggagggaaa attgttaaag gtggtgcctt gcgttctgga gaagatattg
    20701 cgcaagatgg tgcgtttgca tatagacgta aaaaatcgaa tgggaaatac cgttatacgg
    20761 tcttttataa aggacagttt gctctagatt cagatgaaac atcaacgatt gaaggaagta
    20821 aagtaagttt tactcatcca acttggaagg gttcatttgt agatgtacca ggacttggat
    20881 atatgtattc agttgatgaa gatgatgaag gcgtggataa agcaatgatc ggaaattggt
    20941 ttacaaaagt agcaattcca attgaagaag cagaactttc aggaggtaca gaataatgtc
    21001 aaaatatcaa acaacgatta aattaacgaa aaaagacgaa gaaggtaaat atgagcaagt
    21061 acaatttaaa tctgctgaat ttttaccagg aacagttgta gaagatgctg caggagttat
    21121 ggaagaaatg caaacagcga ctgataagca atcagttaaa aaagctttaa gtcgtgctta
    21181 ttcatttatt gcagatactt tatttgaggg acaatttact ggtgaagatt attgcaaagg
    21241 aattgatgct cgtgagattg cctcattgac aggaaaatta ttgaagtctg ttactgcagg
    21301 ttttgatgaa acttatacag agacgaaaaa aaagtaagtg aggcgctcaa gtcaccttca
    21361 tttaagtatt cgattactta ccgagaatta gatataaaga cgcaattact cgaagcaggt
    21421 tggacgttac cagaaattga acgtactgac ttggatgagt taatgcgtct ttttgctttt
    21481 agagatgcgg taaaagagca tgaagatgtt gagtactatg ataatttcac tcaattttag
    21541 gaggtgatac tttgaataac gatgacctaa ttctgaagat gatactagat gaatctggtt
    21601 ttactgccgg tatgaataat gctgtcaaaa agttgaactc ttttgatgaa acaatcgaaa
    21661 gaaaaagtag aaatagtggc agttcattag gtagtatttg gaaaatattt gctggaagtt
    21721 ttctagctag cggagtaact agaatcgtag gagctggctt tgatctaatc aagggttcca
    21781 taagtggagc ggttgatcga gtagatacga tgaataacgc tctacgaaat ttccaaaata
    21841 tgggatttag taattcagaa attatgaaga atatcggaaa gaatgggctt ttatctcaag
    21901 gtattcaagg acttcctact gctttgaatg atgcgataag tcatgttcaa cttcttgctt
    21961 cttctacagg tgatttaact cgttcaactc aaatatttaa agctttaaat gatggaattc
    22021 ttggtttcgg tggttcaact gatcaagtta atgaagctgt tattcaatta tctcaaagtt
    22081 tctcaaacgg aaaagtagac gcacaaactt ggaactcaat gatcaacgct caacttggac
    22141 ctactctttc tgctattgct aaaaagatgg gaattacaat gggagatctg aaagaaggtt
    22201 tatctcaagg taagatttct gttgaagaat tccaaaatca attaatagaa atggatacca
    22261 aaggtggcgg aggacttaaa tcattaagtc aaatcgctaa agattctact aagggaatta
    22321 aaacctctat acaaaatgca aaaacagctg ttacacgtgg tgttggtgag gtaatagaag
    22381 gattaaataa agcattagta gattcagatt taggtggatt taagggaatt attgataaag
    22441 tgggcagttc aatggaatcg ttcttaaaag taattgctgc aaatattcct acagcagtat
    22501 catttttaag taatttattt aacgaaattc aaaaatttgg ttctgctttg aaattcatga
    22561 tgccctttct tgttcctgca gctaccgctt ttggagcgtt catgtttcaa cttaaaggta
    22621 taccagcaat aataaaaagc ttcaataatt ttaagaatgc cataattggc gttggaaatt
    22681 cattaaagat tatgggggca atagctgctg ctaatccatt tgttttaatt attggtgcag
    22741 tcgttggtgc aatagctgtt ttcggttact ttatggcaac caatgaagag cttagaaaca
    22801 aggttatatc tatttggaat gatgtgaaag attccgtaat tggtgtatta aaaaatataa
    22861 aggactgggg aattgatact tggaattctg ctaaagaaat ggcatcaaat gcagttgagg
    22921 gtgtcaaaga tgcttggtca ggaacaaagg aatggttttc taacacctgg aaaggtatta
    22981 aggatggggc cacaggttta tttgataaaa cagtagagac ttcaagaaat gcagtcgata
    23041 gtgtaaaaaa tgcatggtct aatatgaaga aatggttttc tgatacttgg caaagtataa
    23101 aagattcagc cagagaaaaa tggaatggaa tcaaaggttc tattatgtca gttgctggtc
    23161 cattgattac aggtataaag aatgcatttt tacatgttac tttttatcta gagaccttat
    23221 ggaataattt agttgagatt ggtaaaaacg tctttgagat tttaaaaaat gttatacttg
    23281 cgccggtttt gtttattacc tccatgattt ctggtggatg ggaagaaaca aaaaataata
    23341 tgattgctgt ttggaataat attaaagaaa gtgccataaa tatttgggaa tctataaaga
    23401 atatatttgt aagttatttt acaaatattt attttgctgc actcaatatt tggacagggt
    23461 tcaagcttac attgataaat atttggaatg aagtggtaaa tcaagctaaa tcgatttggt
    23521 taaacataaa gtattttttt attaatc
//
LOCUS       DAYYBH010000041        23032 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000041 DAYYBH010000000
VERSION     DAYYBH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.041"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2563)
                     /locus_tag="ACHAUG_001769"
     CDS             complement(<1..2563)
                     /locus_tag="ACHAUG_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8480861.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
     gene            complement(2785..3669)
                     /locus_tag="ACHAUG_001770"
     CDS             complement(2785..3669)
                     /locus_tag="ACHAUG_001770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8480862.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3764..4201)
                     /locus_tag="ACHAUG_001771"
     CDS             complement(3764..4201)
                     /locus_tag="ACHAUG_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480863.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4418..5329)
                     /locus_tag="ACHAUG_001772"
     CDS             complement(4418..5329)
                     /locus_tag="ACHAUG_001772"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8480864.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5706..6344)
                     /locus_tag="ACHAUG_001773"
     CDS             complement(5706..6344)
                     /locus_tag="ACHAUG_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480865.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6348..8330)
                     /locus_tag="ACHAUG_001774"
                     /pseudo
     CDS             complement(6348..8330)
                     /locus_tag="ACHAUG_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            8510..9175
                     /locus_tag="ACHAUG_001775"
     CDS             8510..9175
                     /locus_tag="ACHAUG_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480866.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="ACHAUG_001776"
     CDS             complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="ACHAUG_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFM8480867.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(11723..14245)
                     /gene="pglZ"
                     /locus_tag="ACHAUG_001777"
     CDS             complement(11723..14245)
                     /gene="pglZ"
                     /locus_tag="ACHAUG_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFM8480868.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(14361..17993)
                     /gene="pglX"
                     /locus_tag="ACHAUG_001778"
     CDS             complement(14361..17993)
                     /gene="pglX"
                     /locus_tag="ACHAUG_001778"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFM8480869.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(18057..21728)
                     /gene="brxC"
                     /locus_tag="ACHAUG_001779"
     CDS             complement(18057..21728)
                     /gene="brxC"
                     /locus_tag="ACHAUG_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFM8480870.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(21753..22328)
                     /locus_tag="ACHAUG_001780"
     CDS             complement(21753..22328)
                     /locus_tag="ACHAUG_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFM8480871.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(22328..22927)
                     /locus_tag="ACHAUG_001781"
     CDS             complement(22328..22927)
                     /locus_tag="ACHAUG_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFM8480872.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         6993 a         4531 c         3342 g         8166 t
ORIGIN      
        1 ggatagaaag aacaaattta cctgtatcat caatagttgt ttcggacaaa acagcttgat
       61 tttttttcaa tgaaaccaaa gctcctgctt cgcctgtacc tgtgattgag cgagaagctg
      121 cattgatcaa tgtggtactt aatttagcag gctcaggagg aatcacatcg actgtcgttt
      181 tggcagagtc cagttctgtc acttcttggc cgcctcttac ttttttagca gcaactattt
      241 gcacttgatc ccccgttgtt agtctttctt cgttgggtac ttgaacctca aacaaaccgc
      301 cttgttcgcc ttctccccac ggattatatc ctgtcccttc attttcatct gtttgtcctt
      361 gtgtgagagc agtaatttct acaggtgatt tcgtcgtatc tgcataagta atacgtaaag
      421 tcacttctac ttcgcctgtc caagcatctc ttggctttgc ctcgacacct tcgggaacaa
      481 cgacatgccc atagattttt ttatctgcat tcgttggtac tcttaaggat tcaatcatcg
      541 gaagctggtt attggcactg attcgattat aatttttcat tccggcacct gtctgagcaa
      601 attcatttgt taaacctgca ctagggcttg atacaaaatt acctaagtcg gttccattga
      661 tcgataagtc tgtcatatca gaaaaataat ccggatcttt ttcaaagtta tcgaacgtta
      721 ctcctgcctt tcgccataaa gcaatttctg aatgagcgat cttcaaagaa gaatcgccat
      781 ttccttgaaa tacccagcca cctatgccat tagtctcgct tccgatccgt ttgttttgga
      841 aatcgaaata ggtaggagat tctaagtcta cattcaacac atccccactg atgatgccag
      901 tcttatcttc cccagttttc ccgaccatga ctaaataagt tttttcgcca gccataaagt
      961 tcaaatcaat atctccagtt gtatctacgg tagctccgga atctgcgcgt atatcgatat
     1021 ttgatttttc tccagtaacc gtaaaatcag ctgaccctgg actttctaga aaaccagaag
     1081 cgtcataaaa ttggatcgct tggtttcctt gattgagtct gttcacataa gatttgccat
     1141 cccctgcatt tcttaccttc aatgagcctc cattagttac tgttagtttt tgcgtaccgt
     1201 tctcaaaacg aactgcgcta gaacgaccac tttgcttatc aatatcgatg actgatccgg
     1261 ctccttctac gatcaaagaa agtcctttcc ctacaaaacg catcccattg tttccaggtt
     1321 ggtcgccatc tccagccatt ttgaccgaga tttgtccacc acttgataca tggatttgac
     1381 tagaatctcc ttgcaggcga aatccgccag tagtcgctgt attgattgtg attttcccat
     1441 cattcgacac agcgactctt gatcctgagc ctctcatttg gataattccg cgattgttgt
     1501 cgttggtaat attgatcttt gtatttaccg aaaattcact tcctgagcca ctgactttaa
     1561 atgtgacatt cccgggtagc gtatcgacag cctctccttc atcgctgacg ccatctgccc
     1621 gatcatagtc aagagagacc tttgaaccac tcaagatatg gagtcctggt gctgtttttc
     1681 cactgcccgt ctcatcacct gaatctagaa aaccatagaa ttttcctaga tatggtccat
     1741 aaagctgact accattgatt acatttagct gatagcttct acaaaaataa gctttacccg
     1801 gagcggcatt catcccttct ttcactggtt ggacatccaa tacaccggaa acaccatcta
     1861 atgtgacagt tgcacgaggg gcgtaaatca ggcctaattt agaaccatca tataagtcaa
     1921 gtgtcccggt aaattttatt tctcccgtaa aatgatatct gtttccccaa aaatcggaca
     1981 tataagcaat gataatggca tttccatcga tatttggtat caacccacct ggatgagctg
     2041 ccgttccttt aaaattctga aactcgatcg actgaggtgt ctcattgata tagatgtgat
     2101 gtgtgccgaa attatgagca agtccgccaa aatcgataac gatatctcgc ttgatctcca
     2161 attccctgtc taaagtcaaa ggctgagtta aacgaatctt agctactgat tcgtctgcga
     2221 gaagctgcgt cagttcttct gttgtggcag cttcgctgta cccatccgct gaaacaactc
     2281 taacctgtac ctcttcactg ctatcagacg tttgttcagc tacttctgag gtctcacttg
     2341 tcttattttc ttcttgcctt gtattagaag taggcgcctc actttgggat tctgtagttt
     2401 cagaagtaat tgattcttct gaaaaagtag tcttgtcaaa actttgattc tctgattgcg
     2461 ttgattcgct tgttttctct acatcggcaa aaacggatgt caccatcaag agagggaaga
     2521 caaacaaaat cgacgcaatc agctttttta catattgatt cattctccac catcctctct
     2581 gacgttttga caattttatt ttaacgtttt aatttatttg taattgtact ttcatattta
     2641 aaatatgtat ttttatggca aatttttctg ttaggtgaaa atttcttttt tgtacaaaaa
     2701 aaggctgtga caaaagtctt gtcacagtcc cttattccga acaaccgatg tcctcctgac
     2761 aactcttcgg tcacaacctc cattttaaca ataaacctgt aaatgtgagg gtaacacttt
     2821 gattgtcaca ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc
     2881 atcagctaat gacacaacta attgctcaca agtaagatag gctagatttt tcgtagattc
     2941 atctactcct tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt
     3001 taaataaacc aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa
     3061 accgcctact gagttagtta gtcctatcag caagatactg ctctcaattt cttccttctt
     3121 tccatcaact tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga
     3181 catgaaataa gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattgc
     3241 ttcagggata gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat
     3301 atccaatggc ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg
     3361 tatccctaat gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg
     3421 ccgatgttct tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat
     3481 gacaaacaca ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc
     3541 tgcttttttt gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc
     3601 taattgctca aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac
     3661 ttttttcatc actttctttc ccttctgatt gatttcttta atataacgat agcataaaga
     3721 aaaaaattag gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat
     3781 aaaataccca tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa
     3841 atgataggca ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa
     3901 atagcaagag aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca
     3961 gaggaatacc aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacgacctt
     4021 gaataataaa tgcaacaatc cccgctataa ttagtgtgcc taataccaaa acggaaaatt
     4081 gatgaatata ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa
     4141 aaacaaatcc gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca
     4201 tttatctact tctacgtgca ttttttaata tcgttttctt tatttaagat tatacagaaa
     4261 aatatctagg gtggctggat tctaagtaaa aaaaattttt aaaataaaaa gaactaaatc
     4321 aagtaataat taaactactg ttgatttagt tctaataaaa agatttagat gaacagtttt
     4381 attgatttaa atagttcata ttttttattt ataccgctta tttactactt gctgtactag
     4441 aatctgaagt gaatgtactc aaaacatttt caaagtcttt gtctttgatt tttacattag
     4501 cgtctttcaa tacttcgcca attactttat tctggaaggt actgtcactt aattttgtgt
     4561 ctgttgcaat ttctttcaat tcttttttgt atttatccat gtcgttgcct ttgttttggt
     4621 ttttcaccat tttaaccaca taatattccg tagtataagt tgaagcattg gtagaagtga
     4681 tcacatcaga aacttgacca tctttcaact taaaggctgc ttctttgact tctgccggaa
     4741 ccgttgtagt agtggaatca aatttcactt ttccgccatc ttcttttgtc gtagtatcag
     4801 ttgacttgtc tttggctaat ttagagaaat cgtctccctt atcagcagct ttcttcacat
     4861 cttttgcatc atcttcactt gctacttgga taatttgagc ttccacttct ggatggaaag
     4921 attcccaagc cgtttttaaa tcttcatcgg tcaatttgat atgtttcttt agaccttctt
     4981 gaaaggctag actttgtttg atactgtctt tgaatgtttg ttctgtgtaa cctgcagatt
     5041 tcaattgtga atcaaatgaa tcacccaatt gtttctttgt ttgatcatat tgggaatcga
     5101 tttctttggt tgatacgtca tctccatatt tatcttcaaa tacttgataa acgatcaact
     5161 gttgcacagc ggaatctgct ccttgcattt ttttcaagct atccattact tcttgttcgc
     5221 tgattgtacc gcctttataa ctaacggctg ttttttcacc ggcattcgta cagcctgaca
     5281 ataagccagt gcctagtacg atggcaagtg ctaaaaatct ttttttcatt tatcaattct
     5341 cctacttttc gttttttgcg aattgctaaa aacaaaggtg ttcaagatga aaatcatcaa
     5401 gaacaccttt actaatctgc tcgatacgtc ctgaatagat ataaggctac acaatccaga
     5461 aagttcgctc agcagaataa atgtcaagta gcacatcaca ccttaatata ctacttcttt
     5521 tgttaccgtt tttcaagtct atcttgcgtt ccgtatttaa cgtaactaat tcttagaaca
     5581 aatgttatcg attttgtttt tcttcttccg ctgttgggat attcaaatca gacactttta
     5641 tgttcgattt tccagacatt tttttgttgt ttttattaga aaaagtttgt tttttcattt
     5701 gttttctaaa gcatgatctt cttgtttcct attttggtca gatatggatc atgcgtgacc
     5761 acgataacta acgtaccttg ggaagcctgt tgcgataaga tttcaacgac ttgctcctta
     5821 tttcgtttat ctaatgatcc agttggttca tcggctaaaa tgattttgct attcttcaat
     5881 aagactctcg cacaggcgat tctttgcttc tcaccaccag ataatgtgta gataggcgta
     5941 cttagcttct tgtttatatt tacttttttc aatgcctcat actttgctgt tcgcttatct
     6001 gaaacattta aattcgaaaa cttcaatcca atatctaaat taaaatcgat tgtttcatat
     6061 tcaatcaacc caaaatcttg aaaaagaaaa gagagggcat ttctttttat caagcgcttc
     6121 tctcttccag ttaaatgttt tgtttcttga ttaaataaat aaaattgccc tgaactgaat
     6181 tgatcattca atcctaatat attcaataaa gtacttttcc ctgctccgct ctctccaact
     6241 acagtcacaa aatccccagc atccgcagtg aaacgatcca aatgaagaat cgttttgttt
     6301 cgaatgattt ttttcgcacc ttttatcact atcaacggct ccaattgtta acctccattc
     6361 atcagcaata actgactttt cttttctgta gacaaaataa aaacaactga aagaataaaa
     6421 tcaatgaagc taaagaaaaa aatattaaaa agatatttag gctctctttc tataagaaaa
     6481 aggaagatta cacttgtcgt tccccatttg atgaggttca tcatgaagaa atctttatac
     6541 gtttcaaaga gcgaataacc acttgaacga aagatagcga ttttccgaca attgtagaca
     6601 aaatatatgc ataacatgac atagctcaac aagaagaaag aaaagaaagt gagtaaaaat
     6661 atctgtatca agtaatgaaa tgatctgctt aacactttga cagctatctc ttcagtctga
     6721 gtatacggaa atgataactg caagttatca cgtaactgac tttcttcaat cagctcaaat
     6781 aacctttctt tcgacaaaga gcctatcttg atctttaacg gtggatagat ttctccgctg
     6841 agaatatttc tttcccaata cgtactgttt ttcaatgtca gtatttcaac aaccggataa
     6901 tgttcgatcc aggggataga aggaataaat gtataaatgg gttgattgtt ttttataaat
     6961 agaaatttcg gttctttttc actgatttct tgataatatt ctgttatttg cgccaggtca
     7021 ttttttaaat ctgtatgtct atatcgttca gggatcaata taacagggtg cgtttctttt
     7081 tcggttattt ggatcaacct tccagagaca tcaatcaaag gaaatttctt aagataattc
     7141 agatttattt ttatccctct gccaaactgt tcattttctt cattcaagta atctgataca
     7201 ttgactaata tacttccgtt tttattcaaa gtttcatata attcctgatc ggctgctgat
     7261 gcaaaacgat tggaataaat aaaatccacg tggtttttcc cgatggttac tggataaaat
     7321 acatgatagt cctcaccagc gatttctttt ggtgtttcat gggaaacagc taactgtgaa
     7381 gaagaaaaaa cgatagttgc taattcgaca ttggcaacgg tcaaaagaag agaaaacaac
     7441 aatccaaatg taaaaagaat gtattggata tagttgaacg cttctcttaa cgaagcgatt
     7501 ttttcaacta ataaaagaga aatcaaagag ataccctata ttccgctgaa caagaaaacg
     7561 tacatctgga acaaataaag aaattgctcc ttataataca taaaaataaa accaccaatg
     7621 attcctgcaa ttatgaatgt aataatgatt ttctttttcc ccaataaaca ccacaatact
     7681 tgatgtaatg aaaagccatt attcaacaat aaactgattt ctttattcat caataagaaa
     7741 aaggatgata acaaaaggaa taaaagaatc aatccaatag ttaaatacac tcgataatta
     7801 tatacgtctg tacttgaaat aaccgaatac gaatagctat tttcaaagtc ttcaaaaata
     7861 ttagtggcat aatcagtgtg aaatgcttga ttgaatcgtt ccacaaatag taaaatgaac
     7921 cgttcatagg cttctttttc tctcgtcatc aagaaaaatt caccttcaaa atcttttatt
     7981 ttgatcgatt cttcaatgga cgttacgcga aaggaggtat tagaataggg taccttctcg
     8041 tgagaagatt tgacatcccc ttgataatac aatgtcattt caagctttgg ctgtatcttg
     8101 aagttccaag atgtatctac ttcattggat tcatttactg tttttttcat ataagaaagc
     8161 tcagtttctg atgagacatt ttcaaatacc atgattagct gctcatattc ttcttgccca
     8221 ataaaatgtt gtggcaatcg aatcggagta taagtatctg tttgatggaa aaattcattt
     8281 gtccgataga gtacctgttt atttataata accaaggcaa aaccactcat gataccggtc
     8341 actatgattg tgagtaaaac tgtgaacttt tttttgatta tcaccactct cccttactta
     8401 aatgattctt tgataaaatc ataccgtttt tcatcaaaaa aacgtatcta tcaaatgtat
     8461 aggtcatgta gaataaatct atatactgta gaatcgaaag gagaagaaga tgaacatcgg
     8521 agaaaaaata cgaacttacc gcagaaattg taagcttacg caaaaagaat taggcgaaaa
     8581 aatcggtgtt tccgataaaa caatctctag ttgggaaaat tctcgtacaa tgccagacct
     8641 tgaaatgctg tcacttttac atcagacttt aggtctgccg attgattttc cttctattga
     8701 atcagctacc gaatcaatag aaccaacatt gccggatgaa cctcttgtta tcaagaaatc
     8761 ttattcatca aaacaaccct tctcactcaa aaagacacta tctttctttc tgctcgtctt
     8821 ttgtttggtc tatctttttc ttatcaatta tcaatataaa aatgaaacgg ttgatcgatt
     8881 caatcctttt ttgaaaccac aaatcggcta taccacactc cctgtaggat acgtggaaaa
     8941 ttccatcgaa cattgggtat gtaatgatat ctttgttcat ggtacatggc tgtcttttca
     9001 cgctggagag gtagaaaaag gtaagaatca tgctgtaatc aaacacaaag gaaaacacgt
     9061 catgaacgtt cgcctgctca cttgggaagc cattccctca gattaccaag aaatcctcat
     9121 ccaagactat gacgatgcct taaaaagaag ccctcaggag ccagataggc attaagtagg
     9181 agatgaacgt agagatagaa aaaatcaatg cacatgatct tatcaaaaaa aaacaggggt
     9241 acaaattaat tgaaaaagct ttgagatgaa agttcctaaa taaaaaagtt cagaatgata
     9301 ggtaaaaaaa tgactaacta ttctagtatt aggctatttg tattattctt ctgttatttt
     9361 tatatcaaaa aatccagaaa taactaaata taggtatttc tggcttatag tataatattt
     9421 acgccacagc ccccaatgat tctttttgga atcatgatca aggacggaca tacacaaaaa
     9481 tattttgccc attcgatccg aatagattaa cgactaatct agtaagtaat atagattggt
     9541 acgtagtcaa accagcaaat ggaaaagttg tctattccac cccaagggct ttaaagacag
     9601 catcttccgg cgtttgataa aaaatcaaat taaaggtacc cattaattct gggggtacag
     9661 ttccaatatc agcagcgctt atcataggta caagtacttt tttcgctccg gcatcttttg
     9721 ctacttgtaa acagttagct aaattatcta ccttaatcat agaaccacca attgtaatat
     9781 cccctaaaat aactaaactt ggtaatacag gtttatttaa ggaaactgag cataaagcta
     9841 ttatagttga taacgataat gtttttgtca taccaatgcc attcatatct tgaacatgaa
     9901 cgacaaaatc ttttgtcgtc aaactaatag actgactaac acttccataa ttagctctta
     9961 aatatcggta tgccgaatcc atcgcttctc ttgctccagc atctgaattg atcccagtac
    10021 gttcaaactt tccatttccc gaagtcattt gcgtttccaa tttataggta cctaattgcc
    10081 cagttttgcc ataagatacc gtgtacaaat gccctggctt ccctaaccct tctggaatca
    10141 aagttcctcc accttgttca ggaacagaaa catactcttc tcgctcttct tctaaatcaa
    10201 tgtatgaaaa attgacatca taaaattcca ttccaccaat tttctttaat tgttctttta
    10261 cacgacgacg catttcaaga gcaaatctta gtatttcttc gacatcactt ttttcaaatc
    10321 taccatcagg atacaacagt ttcatcatcc cagatacaat ccgacgaact gcaatcgtat
    10381 cccgctgatt taattgagaa ccaagtttga aatatttatc aaaactatcg ccaaaactaa
    10441 cttttcgaag ctcacgcatg acttcagaaa aataatctgt aatgaagcca tagtcatttg
    10501 taaaaaaatc tggtcgatat ttaggaattt cccaacctgg taaataacaa tgcatccgat
    10561 ccaaaaaggc tgaatccgtc cccataacct ccggaaatgg ctcaaataaa ttcgaagttt
    10621 tcaatattac atcaacgctt tgattaatat ttcctacaaa agccattgat gctgaagcat
    10681 tcttttcttc tttgcctcta gcaaaagatc ctgaagccat gtaatctttc atgatttgaa
    10741 cgccatcatt atccttaaat ttaatcccag caacttcgtc aaaagcaaca cagtcccaca
    10801 tacctacaag tcccattgta cgactagcca tattatagaa aaggtttgct actgttgttt
    10861 gtccacctga aacaaggatt gagtttggtg aaatttcttt atatacatgc gatttacctg
    10921 taccacgtgg tcctagttca cagagattaa aattattttc aattaatgga ataagtcttg
    10981 ctaaatgaag ccattttacc cttttattaa attgtgtagg ttccatacca atggaccgta
    11041 gaagtaagtc aatccattca tttgtagtaa actccttgcg agccatcttg taagcctcaa
    11101 tgtctaaacc aggcatttgg attggatcaa ccttttttgc aataaaaggg ctattccaac
    11161 gatcattctc atcataccaa tattccatcg aaacaataca ccagatcccc ccacctaata
    11221 atcgatcaaa ttttttcgga tactcttcac taatttgaac attttttaat cccaggttag
    11281 agaattctgc ttcataacaa tctttctttt catttaaatg tacagttact ttatcaataa
    11341 ctgtataacc acccaattca cgaatttttg cttttacttt ttctgcttca tcaggacgca
    11401 catagtttcg agcaagaata tttttaacat tttccacacc tttttcaacc aatgtttcat
    11461 ccaatgacga agcatacata ccaagtaaat attccaatac ataaacaggt acattcgcac
    11521 cttctttaat tttctttgtt aaatcttttc tgacaatctt tccgggaaag aattcagata
    11581 actttaaaat ttcctgttgt tccactagaa ttcctccatg tgttcagtca ttataatagc
    11641 aattaaagta tatcatactt aaccagtagg cttgataata caaaagaaaa agtatccaca
    11701 aaaaattgtg gatacctcgt ttttatatat caaaatcaaa tgattctgca aaaggattat
    11761 ctataatgaa ttttattcgc tcggtcaaat catttttttg attttttatc actaaataat
    11821 aagtatcttc aactgtatat cgacgattta caaattcaaa aacaaattta gtaaaccgct
    11881 gatttgcatc ttctgttttt gaatccgcaa catagatttc ttcatttgag atacgttcac
    11941 catattcatc ttcaaaataa agccagaaag atgcggggtc ataaacatca ctaattgcat
    12001 ttttttgata gaattctaac gtggttgata atcctgtaat tctgcggttg ctagtcatta
    12061 attcaattgc tactggttcc tttgcattgc tacctttggc taccgtaatt tctagaacag
    12121 ggactagcat ttcttgaata gatgaccctc catgaacgta gctttgtccc ccgccagctt
    12181 tcttaaaaat acttgaagta atcgggacac aaatgtttct agtgtcatca tttcctaaag
    12241 aatctcccaa agagtaattt ttcgtaccaa ttatttcgta ttcttcttca ctaagaataa
    12301 aacgacgttc tacacgatct ttttcatttc tagatggatt ttcaatctta tctacttctt
    12361 caacgtggct tcttttataa ataaatccat gatcagccgt aactataaaa cgatagatac
    12421 tgccatcatt atgcaagcga ttaataagtc gggaaatttc gcttatcgct aaatcacagg
    12481 cctcaaaaac tcgattttca ttcgagtgat cacctgttct atccacttga tcgtgataaa
    12541 tataaatgac tgatttgcca ttaaatactt ctcgtacctc agccttattc atcttaacca
    12601 catcatcata atttaaagct acagcatttg catctcgttg ctggagaatc gcatttcgat
    12661 tggataaacc attcgttttt ttcccatcaa tcagtacttc tgttccttca ttgtaagtta
    12721 gttgattgtt tggtaataag gcggctttac cgaattcagt aactgaaggt aatacagaaa
    12781 gaaagctagc catgtttgtt gaatacttct tttctctaac taacttacgt tctaaattca
    12841 tccctaactc atatcgaaaa gcatctgaaa taataactac agttttaaca gagcgtttca
    12901 ttacagcttg attataaaat tttcgaatgg atggacgatt cttaaattca aaagtttgat
    12961 tccaaatttg tcctgattga tcaagaaact tcgaataatt tgcttcaact aatcgataca
    13021 attggccata tttttgcttt tcttctatat gatcactatt ccaaataaat ttccgataat
    13081 gcgtatcaac ccaatacatt tccttctcat attgagttag caaattatcc atgttcatcg
    13141 gcaagcacaa tctgttttta tccacaagat aataagcatg tttcaataat tgatactcat
    13201 aaatataatc cgatgcgaaa tgcatttttt cgcgctgctt taaactatca aaaattgttt
    13261 gtcctgcaat tttcgttgta gtgtcagaag cctttaatcg ttctgcataa aacattaata
    13321 tttttgcatg aatctcagaa aatacgtctg cttcaattag atcatcaatt tctatactgt
    13381 ctaataatcc atcaccatca atagcctgat atactttttt agatagctgt tcaaagcttt
    13441 ccatgtaacg tgtatcattc ataaactgat ccatgaaagt aataatgttt gctttcaaat
    13501 gtagtatttc aaatcctttg aacttcttcg gcatctcttg atcaatttga taataaaaat
    13561 agttcaaata aagacacatc gtcagctttt ccaatgttgg ctggctatcc gtaaagccaa
    13621 atttcatttg aataaattcc caaaatttag ctgttaactg atacttatca agttccctta
    13681 aaggattctc cctactttct gtaaagcttt ttagaactac acgcagtaaa tcatttaatc
    13741 gaatactttt cgatttcaac aaaacagcca ttatcattaa ttcaatcgtt tctgatttat
    13801 taattgtcat atgcgtacta aattcaccaa aacttctcat gcgatccttt gcattaaaga
    13861 aattacgata ctgtttaact gtctgatgaa atctaagtgg aatagccaat tcattcatga
    13921 taatcgctaa gcggtctgca ctaaattcct ctgaataatt caatacactc aacaaatgat
    13981 tatcttcatc tttatcatcc caacgaggaa atggcgcata gattaaatag ttagtttcgg
    14041 gatcttcata ttccaataaa cgcttagttt taaattgtgc gtccttttct aatcgataga
    14101 tttttgcatt ttgaatgacc aatgagtcaa tttcttcaag aaaatcctga tttgaatcaa
    14161 accaaaaaat gattttccta catccataat taggaaaacg ttgatttaat ttttcttcaa
    14221 tttgttgcgt atttatatct gtcatcaatc tcacctaatc gttctctttc tatacccctt
    14281 atattgacat aatttggata atttcgcacg caatttaaga ataaaaaaga aagttgttag
    14341 aaaacaactt tcttagataa ctattttttt ggttctttga ccccttttgc aaaaatttgg
    14401 aatttctcgc cggttttagg atcacgctgc aacttatcat aattgacaat tacaccatca
    14461 tctaagtcta attcaattcg ttttaatgct aagtgaccca acttctcgtc atactcacga
    14521 acttctttta attgcttctg aatctttaca atttcttttg tcattgcatt tctttctttt
    14581 gggttatcac ttgttgaaat ttgttgctga cgcaattcga ttcgattgtc atacgctttt
    14641 tgtagttcgt gtaggtattc agtacgtact tttcctaatt gatctggtgt atagcgatgc
    14701 aagtacatta acgctttaaa gccattttgt tttcctgagt ccaattgcca ataaattggt
    14761 cgattggagt atgtttttaa atggtctttg taaaaatctt gtacgaagta acgacgaata
    14821 atttctcgac ttgtcccttt gccacctaaa gtgtcagcga taaactggag attttcttca
    14881 agggtttctt taccaaaagt aatctcaacg aaatcaatga aacgattgac aatgtcaatt
    14941 tggctatcct tgaaatactc atcatcggtg atgagtaaaa cattatcagt atcaggttta
    15001 aatttttgat agcgggaagg atcccattcg ccgccagcga agatgagacc atcttcgtct
    15061 agggagtaac gaccgaagat acaacctatt gaataagaaa taaaatcctt aatttccctc
    15121 gccttatcag gtaaccttgc agagatatat tcatcttgaa cctcttttgt taattctttt
    15181 tcaagagaat aatgattaat gaatatgtcg ttcagttcct cttcattcga ttttagagta
    15241 ttaaattgat taaccaactg cctacttaga cagttaaatg cctctgagag atctttttta
    15301 aacatataac ttaacaaaat gtttcttcta aactcgtatg acgtttcaag agcattatag
    15361 tcttttctag ctatatcgat attttcatcc actttttctc ttattttttt taattttctc
    15421 tcagatgtaa ctttaagaaa aggtagtttt cccattggtc cttcatggta atccaaggtg
    15481 ggagaaataa aagttaaaaa gtgattaact aatttagagt ttacatagcc aaatatttct
    15541 tcaaagtcag ttatatcctt tgagaacaga acagatcctt tagtttcaaa aattgcctga
    15601 ttctgcaatc taaaagatac ttgtccagat gaaagtgtcg accaagtaat tcccttttta
    15661 aaatagtaat taagattttg tggtctagat tttaacttcc cattttcatt aacataattt
    15721 tttatttcat ctccatcatt ctcccagtta accacgtatt cattgtttcc ataccatttt
    15781 ctaaaacgac cacctttagt tgtaggaaac catttttttc tagacttttt agcgtcctct
    15841 aatgttttag catcaaactt tattttgtta aattctactt caaaccaata ccgcaaaaat
    15901 ttattgttat ttccggttgc caacccttgt tttgcatctg caacatcgtt agttaatttt
    15961 ccttgagcaa aatcagtgat tacgttatcg cttgcccagt aagcaatcgg cattcctggg
    16021 atttttttga agttgtcttg attagtacta taaaaataat cgcattcacc atgctgatga
    16081 attgcttcta acgttttaat tttttgaact tccattccac ctttaaaatc tgataatttt
    16141 atatagcttc catcttgttc atttgaattt tttatcgtaa acgtacaaat tggaacggtt
    16201 gcttcttcaa aagctgaata ctccatttga atcaaactag aaatgtttct ttcattaatt
    16261 aaaaatgagc gtaacttttc atagctagaa atgaacatcc aaacaaatgg cgtcataaag
    16321 gaagcatatc cattatcaat cagcatttgg ctattcaatt taataaacac agaaaataga
    16381 tcttttttga catcagaata gttatcattt acatattttt ttaatttctt atccatttta
    16441 ttaagataag gcggattagt gactaccgct acatatttat caactagcat ttcagccacg
    16501 tcaagaatgt gcaagtattt ttcttgtatt tccttcatcc cataaagaat atccatattc
    16561 gtaaattcgt tgaattttga tatatactct cttacttttc ctaagtattc tttagttatt
    16621 gcctgattat ttttatcgac taacttaatg attgatccaa gttctttccc attttcaaat
    16681 tgatcaaaaa ttaactcttt gtattcattc cagcgttcac tatacttatc attgttcaat
    16741 tcactaatac gctcttgata ctcgtctgtc actttaggag aatcaataaa ctctcttaaa
    16801 ttcggcgata gacttttttt caaaaaaaga cgatcgtatt gacgcccttt catcataatt
    16861 gcaaaataag acaattgata cgctctttta tcgatttcaa gaccatataa attgtattca
    16921 acgattgcac gtgcagcatc acgctcagaa tatcctaacg taagataaat ttccatcaat
    16981 acatcgaagg cgtaaactaa aatatgccca gaccccattg cgttatcgat aatttttagt
    17041 tcttctggtt ttaaattact tacagatgtt gcttgttcaa aagcatattt atctggaatt
    17101 aagtatttca acttatttct taattcacta ttgggataat gatctaacca atactttcct
    17161 aaactattgt caaccatata acgaacgacc caatctgtgg taaatagttg agttgctgcc
    17221 ggaatatctt gttttttgac aatcccttta taaatattca ccacttgatt atgtggctca
    17281 ctattatagt actgatacaa ccacccaata atctctacct gtccaccagc ttgaacatag
    17341 aaatcatcct ctggaatact taaaagatca gcgattacac catctttttt aatataagaa
    17401 gctgtaaaca aaagttctgt ataatcttct acttcttcaa atagctcagg taagtaatca
    17461 ttcaattgat tacagatttt tataaaaatc aattgataca gcttgtccat tgcttctgct
    17521 gaattatctg ttttccaagt tctgacttgt tgctgttcat tactagataa ttcttcaaag
    17581 atatcggcat cataaatatc tgtaataata tctggctcaa ttttcccact tatttcacta
    17641 gataatacac gatacgtgtc aggtaaataa tcatttacct ccataaaccg aatggcaatc
    17701 aatcggttaa accacgtata agcaacctct tcgattaact cttgaaaagc ttctgaataa
    17761 gaagtgacag aagcacgttg ttttagttct gcaatcaatt tgtttctttg cgctatttct
    17821 tttccttgaa ttcttattcc tgatttttcg atttcttgta aattgctatc aattttcttc
    17881 acatcttcaa tttttgattc atcaattcct aaacgttttg ctttagtcat tacttcatca
    17941 attaattttc tacgggcatt gaccgcgaaa ttctttatcg ctttcttatc catttttctt
    18001 tcccttctat atgtaacctt tgaaaagaca ggcacctttt tatgcctgtc ttttcactag
    18061 aattgaatcg tgatttctgt attttcttga agttgttcct tcagtttttg attaatttca
    18121 gctgttaact catcaatatc tgctgctgac ttaatagttt taacatgaac gttaagtgtt
    18181 ctcattggta ctgttttctg ttgaatagta ggttttacta tcgctatttc ttcagctttt
    18241 gctgcagagt ctatttcatc tttttcagtt tcacgaatca agtttgctgc ttcaatttgt
    18301 ttacgtgctt gttcttcttg acgtctgcgg ttttcttcat tatcgaaacg ttgattaaag
    18361 atcaaatact gttctgctac gtttaatgat aagaaaacaa gatcatctat agtattcgct
    18421 tgttcaattt ttgctttcag ttcttgaaat tcttggtcaa ctattggtac aaattgatcc
    18481 ttaaaatctt ttccttctaa taatttaaat gttgcttgtt tttcttccgt taatttattt
    18541 aatatttcct gactttccaa ctctaacagt tgattatatt gttcaataaa ttcttgaatc
    18601 aactcattta gtttcatcat atctgaataa ggatcagcct ttttcaaaat ggttttcatc
    18661 tcatgaatca attgatcaat ttcttggcta gaagtttgac gccgactttt ttcgattaat
    18721 ctaattttat cttgtgcatt ctcccagatt tttttctgag tactattttg cttgtggaaa
    18781 tttttaattt tatcatatgt ttcagcccag tcgaataatt catcgcgata ttttttgatt
    18841 gtataaaaaa agtcttcaac atctgacgtt tgaacaatta cttcaagata tttttttatt
    18901 tgctcatatt cataagcact agggtaggta taagtgtttt gtaaatcaaa ataagagcgt
    18961 aaattgtcta attgaccttt taaatatttc ttgaaatttt ccattgtatg ttcattgtta
    19021 ttttcatcaa caatattacg actttgaaac agttctctac cgatttcgtc aaccaatttt
    19081 tttgcttttg gcgaaatcac cgcgcttggt tcaattttta tcttttcact ttctcgtcgt
    19141 ttttgaataa tcattacaat actatttgga tcatccatcc gtgaaatttg tacattgtta
    19201 taaagtaaat caattttacc agacatgaaa agtttagcga tgcaccattc cacatcaagt
    19261 tctgtgaatc catacggttg ttttgtttca aaacgatcac gaattgtttt catcgtaatc
    19321 gttaggtgat ttctcttttg atcattgata aaattgaaaa tttcctgaac tgcttgctca
    19381 tttgatggat cttcaataat ttcatttttg ctttctttca ctaaatataa gatatcttct
    19441 ttttctttag gcgcatcaat ttggtttaat ttatggtata cttcttcagc cattttctga
    19501 agtgctgatt ggaattttgc tttaaagtcg gagcctgtca gttgaatttc tgcaccgtta
    19561 tagaaaaatt ttgcagattt caacagttca tttaattgat cgttgatact tgcacttagt
    19621 tcctgtaatt caccagattt ttccgaacga atcgcgtcaa agttctttac tacactcgaa
    19681 taagtcgttg ttaaataaag attcattcta gcatacattg ctatatcatc tttaaattct
    19741 ttatctgatg gcaattctac gaataataag ttacgggaac tcttaagcgc aagttctgcc
    19801 tcttgacctg ctaaatcaga gtatggtgaa ataaaattaa ctgtcatttc agcatttcca
    19861 gcttgcttgt atggacgttg atctaacact tgtgcataag taaacgtata ccggttttta
    19921 aatttatttc cacctgacat gcctaatttt tcataggcaa aactatttcg gtctagaacg
    19981 ccgtcaaaaa ttcgtttaga tagttctacg atcaaatcac tatctgtaat attttgattt
    20041 ttaatctcac gagtaatttc ctgttcttca tcagtttgga aaatataact atctaagttc
    20101 ttccgcacaa agttttcctt aattaaaagg tccaatgctt cttctactcg ttttttagtt
    20161 tccaggcggt cttcgttgat atttcttatc atcaagctcg taatattttc aacatttggt
    20221 ttgatggctt gatcaacata tttaatcatg aataaaattc gtaagacatt tacgttaaag
    20281 ttatctatat cagttggtct ttgaatatga gaattatttg tagcttgtgt aatcactcgg
    20341 ctaattgctg tatcaatcca acgttctaac gagtaataaa actgatctaa agaaaccaaa
    20401 ctggttgtat cattttcctt atttcgttca gcggcttctt taaacattgc tagtaatgaa
    20461 cgttcaccag acgataagtt tgatccttga tagctgtgat tacgaatctc ggttagaatt
    20521 tgccccaata aattgaattg atagggaata aatggataaa cttctgcgta attttcagca
    20581 tcttggaaga gctttaattc aggactatca acaaagagta atttattttt caattctgtt
    20641 tgaacagttt caaattgtaa ggctaaagaa tcattggcaa actctttttt cttcaaaata
    20701 cgttctttaa ttacttcatc ggcatttgct gaagataaac tcacagttgt ggcaaaacga
    20761 tccttgatct tagaaaaatc attatcagaa ttgttatcaa agtttgttac gtgatcaatc
    20821 gcttcctgac ttgtcactaa tacccaagcc ttaccttcac aaattcgacc caaatcttcc
    20881 acaacggttt gtaactgcaa agtcaactca cgattaccgc cgatatattg tccgacctca
    20941 tcgactagaa aggcaatatg atggtctgct tctttggaat caagatagct tttaactaga
    21001 tttgcaaagt catcaatgct tattaaataa tttcctgatg ctttttctaa aaaattatca
    21061 atagcggctt cactcataaa atcgatttta agtgccgctt ctttgatatc atcttggatc
    21121 caatcaaact catggcgctc ttcttgccat tttgccccat taatctcttc aaaagcagct
    21181 ttgaatgcat caaacttttc ttctttaatt aaccgacgtt caaaatctgc tagatgtgga
    21241 atcgtgctta atccttgcat ttcgttaaat acctttaaaa aaacattcac gattccgttt
    21301 ttgtcttttt taccacctgc atcacttttt gaatcaatat taaacagaat aacatctgta
    21361 tttatttgtg agcaccgttc catcgttgcc aaagtcagtg gatctttgat tttcccatct
    21421 tctttgaagt aatcaatggc tttcttgtca tttatttctc gatttgcgag caagtaagaa
    21481 ataattttta agaaatgaga tttacctgaa ccaaagaacc cctgaatcca aacacccatt
    21541 ttatctgttg gtgtattgat actccgttgg taggcatcaa aaattgtttt aaagtgtttt
    21601 tgtagttctt tcgtaaccac gtattcttct aattcttgtt gaacattttc atcctttgct
    21661 tgaccgacct taataactcc ttgaatattt cggtcaattg gttttgcaaa aatatcttta
    21721 attagcattt aatctcctac cttccatccc aactaaattc taaatgctcg ataataatta
    21781 tcatctttat tggtattaag aattgtcaaa cttaattcat catattcccc tggatacatc
    21841 atcacaactg gtacgcgatc aaatacctga tgcattttat ttaaaataga gtgcgctcta
    21901 attaatggat ataattcgcc aattcctgtc aggaacacaa ctgatttttc tggtgtatgg
    21961 gcatccacat attcaaaaaa tacattttct tcctcaggta aatccatttt caataaacga
    22021 ccaatgcttt ccactaattt ttgtaaacca cgtcgttctt ccatcttaat cgctttttca
    22081 gtgaaaccgc gtttttctag atactctaac ataatcacat agagattata aactttcact
    22141 tcataattat caccaacaga tgttccagat atatcattaa tataatctct tacaattaac
    22201 tcgtctttcg gatcgtagtc aaagatataa tagcctactt cattacctaa accacgattc
    22261 aataaaaaat cttctttttg aattacttca ttcaactttt ccaaatgctc ttgaatcgat
    22321 aatgacatta taaaattcct cccagtgcta gtacaatatc tttacgattc ttgtcaacta
    22381 accattgaat caatcgttta tctaataata tcggagtcat tcgtccatat tctttttccc
    22441 ctctgactaa tcctgtttca attagattcg tttgataagc ggcaataagc cgtttaatcg
    22501 tataatcttt ccattttgaa atatcttctg aacttctccg cttctctgta aaaaaagaac
    22561 ggtaatcaac tggtgttaat tgcaaatttc ctttacgtag attttcagca tatacctcag
    22621 tcataaattc attaaaaaga tcatcaatca taaggatgga aatcaaattc actaactttt
    22681 gattatctat atttaactga ttgaaaagtt gcaatccagc ttcatccagt tgactaactc
    22741 tccttttcac gttctgatag atttcttttt gacggttact gctcactgca aaaaaaatat
    22801 tttcatgttc atttttctct ttaatttcta tatctgtata tccttctagt cgtaattgaa
    22861 tatataactt gaactctgaa aaccaaaagc gtttagaaac tagaccagca gaatattttt
    22921 tattcaatag attgcctcct aatcatctat aattcaataa gtaaacaagt gttttttgct
    22981 catcgttagc attcattttt tagtatctac tatacaatac atatttatta ta
//
LOCUS       DAYYBH010000042        22906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000042 DAYYBH010000000
VERSION     DAYYBH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.042"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..144
                     /locus_tag="ACHAUG_001782"
     CDS             <1..144
                     /locus_tag="ACHAUG_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8480873.1"
                     /translation="YLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDK
                     SQF"
     gene            248..394
                     /locus_tag="ACHAUG_001783"
     CDS             248..394
                     /locus_tag="ACHAUG_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFM8480874.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            470..1717
                     /locus_tag="ACHAUG_001784"
     CDS             470..1717
                     /locus_tag="ACHAUG_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8480875.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            1732..2484
                     /locus_tag="ACHAUG_001785"
     CDS             1732..2484
                     /locus_tag="ACHAUG_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8480876.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(3187..3402)
                     /locus_tag="ACHAUG_001786"
     CDS             complement(3187..3402)
                     /locus_tag="ACHAUG_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFM8480877.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(3421..3657)
                     /locus_tag="ACHAUG_001787"
     CDS             complement(3421..3657)
                     /locus_tag="ACHAUG_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFM8480878.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            3915..6068
                     /locus_tag="ACHAUG_001788"
     CDS             3915..6068
                     /locus_tag="ACHAUG_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFM8480879.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            6037..7452
                     /locus_tag="ACHAUG_001789"
     CDS             6037..7452
                     /locus_tag="ACHAUG_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFM8480880.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            7485..8519
                     /gene="sppA"
                     /locus_tag="ACHAUG_001790"
     CDS             7485..8519
                     /gene="sppA"
                     /locus_tag="ACHAUG_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8480881.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            8521..9042
                     /locus_tag="ACHAUG_001791"
     CDS             8521..9042
                     /locus_tag="ACHAUG_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8480882.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            9039..9425
                     /locus_tag="ACHAUG_001792"
     CDS             9039..9425
                     /locus_tag="ACHAUG_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8480883.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(9921..9993)
                     /locus_tag="ACHAUG_001793"
     tRNA            complement(9921..9993)
                     /locus_tag="ACHAUG_001793"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9958..9960),aa:Lys,seq:ctt)
     gene            10374..10943
                     /locus_tag="ACHAUG_001794"
     CDS             10374..10943
                     /locus_tag="ACHAUG_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8480884.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            10930..11790
                     /locus_tag="ACHAUG_001795"
     CDS             10930..11790
                     /locus_tag="ACHAUG_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8480885.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            11980..12684
                     /gene="yycF"
                     /locus_tag="ACHAUG_001796"
     CDS             11980..12684
                     /gene="yycF"
                     /locus_tag="ACHAUG_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8480886.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            12689..14524
                     /gene="walK"
                     /locus_tag="ACHAUG_001797"
     CDS             12689..14524
                     /gene="walK"
                     /locus_tag="ACHAUG_001797"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8480887.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            14521..15837
                     /gene="yycH"
                     /locus_tag="ACHAUG_001798"
     CDS             14521..15837
                     /gene="yycH"
                     /locus_tag="ACHAUG_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8480888.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            15838..16707
                     /locus_tag="ACHAUG_001799"
     CDS             15838..16707
                     /locus_tag="ACHAUG_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8480889.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            16762..17571
                     /locus_tag="ACHAUG_001800"
     CDS             16762..17571
                     /locus_tag="ACHAUG_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8480890.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(17674..18303)
                     /locus_tag="ACHAUG_001801"
     CDS             complement(17674..18303)
                     /locus_tag="ACHAUG_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8480891.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            18473..19765
                     /locus_tag="ACHAUG_001802"
     CDS             18473..19765
                     /locus_tag="ACHAUG_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8480892.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(19841..20503)
                     /locus_tag="ACHAUG_001803"
     CDS             complement(19841..20503)
                     /locus_tag="ACHAUG_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8480893.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            20728..21012
                     /gene="groES"
                     /locus_tag="ACHAUG_001804"
     CDS             20728..21012
                     /gene="groES"
                     /locus_tag="ACHAUG_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8480894.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            21063..22688
                     /gene="groL"
                     /locus_tag="ACHAUG_001805"
     CDS             21063..22688
                     /gene="groL"
                     /locus_tag="ACHAUG_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8480895.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7856 a         3623 c         4550 g         6877 t
ORIGIN      
        1 tatctatatt ttactgctta tactaataaa ataagattca ctgaatatca agaggaatta
       61 ataagaaatt tgagtgaaat tggaagaact gctggaataa atggtttata tcgagcagat
      121 tatggagata aatctcaatt ttaatgatac ttataatatt aaatatgtct caaaattttt
      181 tgattagaaa aataggtata ttgaatcagt gacataaaag tatttatagt ttgtaggggg
      241 aatcgtaatg gaagaaaaaa atagactaaa tgctaaacaa tgttcagatc aagagcttaa
      301 aaaaattaaa ggaggtgctg gaacaaaacc tcaaggaaaa ccggcatcaa atttagtgga
      361 atgtgttttt tcattattta aaaagtgtaa ctaaaatttt ttttatgata tcctaaaatc
      421 aggaggggat ctcttgatta ctatcgatac aaaagcttat ctattaagaa tgttcgtggg
      481 aaactttttc ttttttctct tattttatat gactaatact gatcttaaca aaagaaaata
      541 tctttttgta tgtttattga taccactcac tttttttgtt gaattattta ctgatgtttc
      601 cgcagctata cctataatcg gaagttattt tatattaaag gaaaagaaga aaactaatat
      661 agtgctttta aataaattac tattatgtat gcttattgat gatttcagat ctattattac
      721 ttccatggtt atgacgtata tgttttcatt tgaagggata aaagggtatg gttatgtagt
      781 attccagata atgcttgatt ctttgttttt gctgatgttt atttggttat atagaaaatt
      841 agctataaat aggattgttg aacagtccag ttctaaactt actgctatat tcatgattta
      901 tttacttaca attggtttat ttgtttctta tgttgctcat caatatcaag tatttgatca
      961 ttttgtgttt ggtgtcttaa tatttttaat cattcaaatg tttgttgttc tatttttatt
     1021 tattagaata actattaggc aacgagaaca atatgaacag caattgaaaa gaaaagaatt
     1081 ggtttatcta aaaaaatata ctgattcact ggaaaaagat caagaaaaat ttgccagatt
     1141 tagacatgac tataaaaatt tattgctgag tttaaaagaa atcgctaatc aaaaccatga
     1201 ttcgatattg gaaaaagaaa taaaagaatt agagatctat tctaattctt atctaaccaa
     1261 taagtttgaa tatcgttatt taaaaaatat aaaaaatgaa tatttgaaaa gtttgttgat
     1321 agcgaagcta taccaagcaa ataacaataa gatttgttgt aaattcgaat gtccaagtac
     1381 tattattgaa gttcctgttc caatatttga ctgtattagg gtgcttggaa ttattctcga
     1441 taatgcaatt gaagcatcta tagagagtca agatagagaa ctgtctctag ctatttaccg
     1501 agatgataaa cagatagaaa tttcgatatc gaatagttgt agagagatac tactctctat
     1561 agatactcta atgaaaaaag gaataacaac aaaaaaggga catcaagggt taggtttatc
     1621 tagtatagag gaaatcaata aaaagaatcc caatatgttt gtgaactatc aaaacgatcc
     1681 gtatcaattt actactcaag ttattttgtt gttttgaggg aaggaggatt tatgagttat
     1741 ccaataatat tatgtgaaga tcaaattata caattaaatc agttagaacg aattattgat
     1801 aatttcattc tttttcacga taaagtattt aagatagtat tgaaaacaca aagtccgtta
     1861 gaagttaaaa aatacctcaa acaattccga ccaaagcaag gaatatattt cttagatatt
     1921 gatttaaatc atgaagttaa cggtatagaa ttagcagaag taatcagaaa atatgatgtt
     1981 caagcaaaaa tcatttttac aactactcat gatgagatgt tacccgtaac aataaaaaga
     2041 agagttgaaa cgttaggatt tgtaacaaaa gatcaaacac tagatgagta tcgaaacgag
     2101 attgttgagt tattgttatt agcgcaagaa aggatagatg caacaaaaca ggttacgaat
     2161 caagcatttg ttttttccat aggttctcaa acatttactt ttgatataaa tgacatttat
     2221 tttgtagaag cttcaaaatt accacataga ttatcattgt gtactaaaga tgggcagtac
     2281 gaattttatg gaagaattag tgagcttgaa aagaaatatc ctatgttaac aagaattagt
     2341 cgagcgtgtt tagtaaatat ctttaacgtt aaagaaatag attttaaaaa acgttcacta
     2401 tattttgatt cagagttggc cagaaacttt actttaggga aagcacaaaa aattaaggag
     2461 aagttgaaag aaagcactgt ttagtggttt tttctttagg gagattgcat ttttctaata
     2521 aaatagattc cttcactagt gcaaattttt ctgaatccta ttaaagttgt accaattttt
     2581 atagctgtta ttagttacgt gcataacagc aatattagtt actggaggaa tatttctatg
     2641 gtctggaacc aaaaaagggc caaatgaata gttcttttct tgtttttatt aagtaccata
     2701 aaggggaatt aaaattaaat gtatggtgga agatgcgaaa gatttggcaa attctactaa
     2761 tttttagaaa gtgtttttca ttttttagtt aaaaaatttg tcctgtcaag tgcaatccta
     2821 tgattgtact tgataggatt tgagataatt actttcctgt ttttataaat ttaacttgca
     2881 ggaacaggct ttaatgcaca tccgacaagt acattaccaa tacctcctgt aacggccgaa
     2941 ataggtcctc ctgcagcacc aactacagcg ctgcaagtcc ttttgtaaaa atatctctac
     3001 cattacattt ccaaagaatt tttatctgac tttaataaaa gataaaaatt taatataatt
     3061 ataaaaacta gcacaagaag ctttagtaaa aagttctgtc cattccaaac ttttgaaaag
     3121 ataaagaaca tacctatgtt cgtaatcgac acaaaccaat cttgaggttt gatatttttt
     3181 aattgtttac gcaccggtcc acatgctgaa tccagcacct aataaagctc cacctacacc
     3241 acaagcagct attgcataag gtcctccggc tgaacaaagt tttactcctg ctgttgcccc
     3301 agaaaaagta gtgcctaaaa ctcccacggc actcccacct tgaatatttt tcaactcttc
     3361 tgaagataat ataaatgttg tttttgtgtt tttaatatgc atactactta ttctcctttt
     3421 ttattttttt cgagcatccc agccagctgc agcacctcca gcgacagctc catataaccc
     3481 tccaacaaca ccgcaagctg gtccgcaaat tgctgttcct aaacctgcac cacctactgc
     3541 ccctgtggta agtccaccta caacttgccc caattttccc caagcattat tgcctccacc
     3601 aacagtttgt gcatttccac aatacttagt tcctttacat tttgcatgtt taaacacgct
     3661 tctttaaata atatttttta cggtgctact gtaacatacg tatttgatta acagtaaaaa
     3721 tcttcctgaa acttatcaag tttattcttg aatagtaaaa tttccaaaat aaatttgatt
     3781 caggaatgaa aaagttagtg ttcaagaact gttttcaaag tttttgtgga attatgagac
     3841 aatattttca ctgtattaaa gctatattga gaatttttct ataaaaattt tttagaagga
     3901 gttgtgttag ataagtgata aaatcatttc cttttgtgca gcaacaagat gaaaaagatt
     3961 gtggtgttgc ttgcctttcg atgattttaa aatattttaa aactgatatc ccgattcata
     4021 aattacggga cctatctggg actgatttag agggtacttc agcatttgga ttgaaaaaga
     4081 catttgaaaa attgtctttt gattgtatgg ctattcaagc agatagtgag atctggaaag
     4141 aaagagatct tatcttacca ttaattgcac atatattggt tgataacagc tatatgcact
     4201 acgttgtaat ttataaaacg aagggaaacg tactatggat tgcagatccc gctaatggaa
     4261 aaaatagaaa aacgattgaa gcgtttcaaa aagaatggac aggtattctt ttattaccta
     4321 tgcctagaga aacttatatt cctattaaag aaaaagtagc tggattaagt tcttttttgc
     4381 ctataatttg gaaacaaaaa ggattagtat tccacatcgt tttagcatcg attttcatta
     4441 cgttttttgg cataggaagc tcatattatt ttcaaggtat tcttgatttt ttcatcccta
     4501 atcaagcacg ttcaacttta aatataattt ccattggctt gatcgctgtc tatttatttc
     4561 gggtattgtt tgaatatagt cgtagttatc ttcttattat tttaggacag cgaatgagta
     4621 tggcaattat gcttcagtat ttcaaacatg tgttaacttt accaatgaat ttttttgcta
     4681 cgagaaaatc aggggaaatt atttctcgtt ttcttgatgc cagtaagatt attgatgctt
     4741 tagcaagtgc tacgttatca atatttttag atattggtat ggtgttgctt gttggggtta
     4801 cgttagctat tcagaatagt acgttgtttt taatcactct atgctctttg cctttttatg
     4861 tagttgcaat attcgctttc gtgaaaagct atgaaaaatc gaacagggaa gaaatggcag
     4921 ccggtgcagc tgtaaattct agtatcatag aaagtcttaa aggaatagaa acaattaaag
     4981 catatagcgg tgaagagaaa gtatataacc gtatagacca tgaatttgtt aaattaatga
     5041 aaaaatcatt tcgtacggtt acgcttgata atattcaaca aggaattaaa catttgattc
     5101 aattaattag cagtgcatta attttatggc taggttctta ttttgttatg gataatagaa
     5161 ttagtctagg tcaacttatc acatacaatg ctttactagt atttttgaca gatcctttac
     5221 aaaacattat taatttacaa gtgaaaatgc aaacggctca agttgcaaat aatcgtttaa
     5281 atgaaatttt cgcgattgat tcagaaaaat cgaaccagtc ttttgaatat atgctttctg
     5341 aacaattatt tcaacaagat atcactgtaa aaaacgtttc attctcgtat agcatgaagg
     5401 ccccaacgtt gaacgaaatt tcatgtacaa ttccttcgca cagtaaaacg gctatagttg
     5461 gagttagtgg ttctggaaaa tcaactcttg caaagttatt tgttcgtttt tatgaagcat
     5521 cagaaggatc gatccattat ggaaacatta atcacttaga cattcctttt cagatattac
     5581 gcgatcgagt gacatatttg cctcaagagt catttttctt tagtggaaca atcattgaga
     5641 atcttatatt cggtttagat agcccaccaa gtttcgaaca aataatagaa gtttgtgaga
     5701 tagtccaact aaatgaattt attaatcaac agccattacg ttacgagaca ttgttggaag
     5761 aaggtggaaa taatctttca ggagggcaac gacaacgatt agccttagca cgggctttat
     5821 taaaaaatcc ggacatatta attttagatg aagcaactag tggattagat accttgttag
     5881 aacatgcaat tatgaattat ctcctcaatt taaaagaaaa aacgattatt tttattgcgc
     5941 atcatttacc aatagcaaaa gcatgtgatc aaattttagt tctccatgaa gggcatttgg
     6001 tagaaaaagg aacgcacgat gaacttcgtt ttgccgatgg tatctatcaa aaattatggg
     6061 aaatctagga gagggaaaca atttatgcag aataataatt ggttcgaaca ttctagtatc
     6121 tatactaaac aacatcatag cttctaccgt tgggtgttat accctgtaat tcttttttta
     6181 tgtattattg gattgttttt aacctttgct aaaaaagagg ttgttattcg gacaacggct
     6241 caattgaacc ccgaaaaaat agagaagtta caagtgccat tagaagctaa aataatggaa
     6301 aacttcctaa aagaaaatca atttgttcat aaggacgatg tgttggttag attggattgt
     6361 tctttaatag agaatgaaaa ggctcagata gaacaagaaa accaacgtat tactcaacaa
     6421 ataaagatgg ctcagctatt tattgaaagt ataagtaaag gaaaaaattt gttttcaacg
     6481 gatgacagtt ttggctacag taatcaatta aagagcatgt tgtcagaaaa agaatcactc
     6541 cgctacgctt tgaagcaaag tgaattaaat gatcaaaagc aattagaagt atacgaaaag
     6601 acaaaaagac aactagaaaa acaaattgag agttcagata gtaaattaca agaatggcaa
     6661 caagtacagg tagcttggag taataatcaa tcattaaaag atttttcaaa agaaatgatg
     6721 gcaaactatg agaattggca agaacaacta aataatgttt ctgatgatca aaaaaatcaa
     6781 gtgaaactga caatttcagc aagcataaat gaacaaattg agcaactaaa aaaagaagta
     6841 gaacagtatc agtcagaaaa agctaaatta gttaaaccaa ctacttctga gaatgacaga
     6901 attagtcaaa cggaaaaagg aaagcaagag ctagaacaag caataacaac tgtaaaacaa
     6961 acagttacag agctaaatga aaaacaagag aagaaccagt cagttatcaa aagtttggac
     7021 gatcaacttt caaaaggaat actaaaagct cctgtatctg gaacggttca tttaaatgaa
     7081 gaaacaaagg gacaagcaga actagccaaa ggaacagtgc ttgcagaaat ttatccgatc
     7141 cacgaaaaat caaaaatgaa atttacagca cttttacctg cgaatgaaag tacctatatt
     7201 aaagaaggga tgaaggttca ttttaaattg gatcaaaaag gtggggctcc gattacgatc
     7261 gacggaactt tagatgaaat ttcagaaaat tctactgcca ctgagaaagg tgttttttat
     7321 gcagtgaaag gaacgttaaa aacaactcaa tcgtttccgt ttcgttatgg attaactgga
     7381 gagttgtctt taattgtagg agaaaaaaca tattggcaac aaattgtcga tgtgtttcta
     7441 aataaaaaat aattattttt actaagaata taggaggagt ttatatgaat aaaagaagat
     7501 ggattgctgt gggagtagcg tgcggattat tgttactatc cattatcgtc gcgctaattc
     7561 caggaaaaga taaagaggaa gcatcaaata caaccctaac aggaataaat aagatttttt
     7621 acggatctaa cgaaatcaca gaagaaacgt tagaagaagg cgcatcaaat aaaaagattg
     7681 tcaaactatc cgtcaatgga gttattgcag atacaggtga aagcaatctt tttagtaggg
     7741 aacaatataa ccatcagaat tttttaacac agctaaagaa aattcaagaa gacaaagcgg
     7801 ttaagggagt gttattagaa gtaaattcac ctgggggagg aatatatgaa agtgctgaga
     7861 ttgctaaaga aatggcaaaa attaaaaagc tagatattcc tatctatact gcatttaaaa
     7921 atacagcggc aagtggaggg tactacattt ctgctggttc agataaaata tttgctacgg
     7981 aagaaacaac aactggatca attggtgtca ttatttctgg tttaaattat tctggcttat
     8041 tagaaaaact tggcgtaact gatgcgacgt ataaaagtgg tgcattaaaa gatatgatgt
     8101 cacctcaaca caaaccttca gaggaagaga ataaagttat acaggaattt gtgatgagtg
     8161 cttatgatcg ttttgtcaat gttgtagcaa aaggaagaaa tatggatact aatgcagtga
     8221 aagaacttgc ggatggaaga atttatgatg gtaaccaagc agtagaaaat gggttggtgg
     8281 atcaaattgg ttactctgaa gatgcattag attccctaaa aaaagagaag aaattaactg
     8341 atgcgacggt tatagaatat aaaaatgaca caactggctt tgcttcttct tggttaggta
     8401 ataagattgc tgaatggcaa gggctaaagg caactacatc agatcgtgta atttcagttt
     8461 ttgaaaaact tggattttct gatactccaa aaccaatgta ttactatgga gggctataaa
     8521 atgaataaga agagtggcat tgaaactttg cctaattatg tatttgcggg attttggtta
     8581 cgattttttg cttttcttat tgatttaatt tgtattagca tcatcaccca tgctactata
     8641 ggtctctttt acaaaatcat gggactggaa tctgacaaat ccattattac tttttatggt
     8701 atagcgtcat tagctattta tttaacctat tttgtacttt tgactaagct gaattcagga
     8761 caaacaattg ggaaaatgat ttttgggata agagtagtga gctttgagga aaaagagcta
     8821 agttggcaaa ctatctttat tcgtgagtgt atatgtaggt ttatattaaa aaattttgta
     8881 tttcaaattg ggtatttggt ggctgctttc acgcccaaga aacaacatat tggggattat
     8941 ttttctaaca cgtcagtggt tgttattcgt tcattagaag ctattaacta tggtcaaaaa
     9001 atttctaatt ctaaaacact taatctggag agaattaaat gaataagaga aaaacagagt
     9061 ttattttgat tttaattagt ggaataacag ggattttatg tagtctttta gcattgatgg
     9121 gtggtatgtt tctatcttct attgattcaa ctactttaaa tactacagaa ctaaccagta
     9181 aggatataga tatgttagtc aatctaagcg ggaatgttgt tttatttgga ggaattaccc
     9241 tagttttttc gataatgatt ttcataatag gttttttagt aagaaaaaat caaaaaacag
     9301 tggtctatgg tattagttta ctcattttat ctattattcc attttttctt ttaacattat
     9361 tgtgggtaat tcctggagtt ttaggtatta ttgcgggatt gatgcttcta ataagaaaat
     9421 cttaattagg caatgtgtag acaacgtgaa acgattaatc aattaaaagc taaatacgtt
     9481 ttgagaaatg ataacttgta gtcgatggaa gacatgtaga taaaaaaact acgtgtcttt
     9541 tatttattct aattttttcg aaaaaaagac actctctgat tgttgtatta atggaaaagg
     9601 ggaaagcagc aagtaaattt agtttttcta ttaaatattc ttcacttttt aaccttttat
     9661 agtacttaga tttaatccta tatgtttaga gagttcattg gcagcatgat tttgcaaagc
     9721 ttctcgaatt attgttatat tagaaacttt actgttcata attatgcttc ttttttaact
     9781 caaagtccaa agggctacag tggactttga gttaaaaata aaaagcccta gttattttga
     9841 aaaataaaaa aagaacagcg taaatgtttt cgaaatgctt tcgcaccaac atttacctgt
     9901 tccgtgggtt tggcacccaa tgggccgtga ggggctcgaa cccgcgaccc gctgattaag
     9961 agtcagctgc tctaccaact gagctaacgg cccgtacaaa aagtacttaa taatagtagc
    10021 actaagaatt ttaaaatgca agactacatt taaagaaaat cagaaaaatc tttacttcct
    10081 tataggaaat cattcataga ttattcacat ttgctcgcta ttatataagc ataccaacaa
    10141 acaaagttct tgcaagagaa caaaccctaa cacttttttc attttttact cctttcccgc
    10201 cgtctccccg gcgggtctcc tgttcttaac gataaaagac aagaaggctt agccttcttg
    10261 ttttttgttt tcatggaatc ccgctaatca aatcacctag ttaaagcaac tagattgaat
    10321 gtttatcata agtctgatat catgtttatg aaatgaaaaa aggagcggtt atgatggaaa
    10381 aaatgcgtca gcaattaatg gagtggggga aggaactaga aacattccgt ctgcctcact
    10441 gggaagaact ccctgactta gaactttata tggaccaagt cagaacgtta gttgatcgtt
    10501 atctttcacc tgtgatacaa ggagaaaagc atccattgct tacctcttct atggtaaaca
    10561 attacgttaa actaggtttg ataccagcac cagttaaaaa aagatataac aaagaacacg
    10621 tagcattttt acttgcaatc actacgttga aacaagtatt gactattcct gaaatcaaag
    10681 aagggatatt gttccaagga aaaacagtag gaattcgtga agcctataat ctattttgcg
    10741 atgaacaaga agcagctgtg tggatggtca gccaattagc acaaggtaaa tctcatccgc
    10801 aaaaattcga ccaggcaacc cctgtagaat atattgctgt aagagcggca actttatcgt
    10861 ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga aaccgaatat ttaaaagagg
    10921 agaaaacaaa tgaaaaacaa taaaatcgca attttagtag attcaggtac ggatgttcca
    10981 gaagaattga taaaaaaata cgagatgtat atgatcccat tgaaaattat ttataaagat
    11041 caggtatata cagataaaat tgacattacc cctgaagaga tctatcaacg tttgccagag
    11101 gaaatccctg gtacatcttt gccggatggt gagtcgatca cagaaatctt tgagcaaatc
    11161 aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt gagcgggaca
    11221 tataatgtgg ttcgtttaat tgcacaacag caagaagaat tggaagcata tgtactagat
    11281 acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa atggctggaa
    11341 gaaggagtag aatgggatca actgatcagt aatctgaatg agttagtcga aagaacaaaa
    11401 gttttcttta atgttgcgac attggaatat cttcaaaaag gtggacggat cggtttagtt
    11461 gcttctattg taggaaccgc tttaaagctt aacccaatca tctcatgtaa tgaagaaggg
    11521 atttattaca cagtaggaaa agctagggga cgtaaaaaaa gtttggataa aacggttgct
    11581 tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc ccatggggat
    11641 gcgaaagaag aagcaattga aatggaagcg agacttaaaa gagagttccc acaagcaaac
    11701 caaatctact ttggccagat ttctccggca ttagtagtcc acactggtcc gggattattg
    11761 ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa ggaaacactg aggtgaaaat
    11821 cagtgttttc cttttttatt gaagttagaa caaaaaataa taaagtattt tgcactttca
    11881 ttttaatttt ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa tacggtataa
    11941 tgagaaggta tgacaataat atataagagg tggctcttta tgaaaaaagt tttagttgtc
    12001 gatgatgaga agccgatttc ggatatcgtc aaattcaatt tagccaaaga aggctatgac
    12061 gtttatactg catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt agaacctgat
    12121 ttgattttac ttgatttgat gctacctaaa atggacggct tagaagttgc tcgtgaggta
    12181 cgcaaaactt atgatatgcc gatcattatg gtaacagcca aagattctga aattgataaa
    12241 gtcctaggct tagagttagg cgccgatgat tacgtgacca agccattttc caaccgagaa
    12301 ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa ggaaccagaa
    12361 gaggcagcac ctgcggaatt gacgattggt gatttgacga tccatccaga agcttacatg
    12421 gtgacgaaac gaggagaaac aatcgagtta acacatcgtg aattcgaact actcttttac
    12481 ttggcgaagc accttggaca agtgatgaca agagaacatt tattacaaac ggtttggggt
    12541 tatgattatt ttggagatgt acgaacagtg gatgtaactg tacgtcgttt gcgtgaaaag
    12601 atcgaagata atccaagcca tccaaattat ttagtgacac gtcgtggtgt cggttactat
    12661 ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa gtccgctttt ttcgatcagt
    12721 caattttaag atcgctatta cctttattct gattttattg atctcgatcg aaattattgg
    12781 tgcctacttt atacgtggtt tagaacgttc cacgatcaat acatttatta aagatatgaa
    12841 tcaaacggtg gaatcattag caacaacaat cagcccggaa ttgaatcgaa aagataatgc
    12901 agatgatgaa gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg cgactagcga
    12961 tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa ccgatgtcaa
    13021 tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat atcaatgatt tcacgactaa
    13081 acgttatgtt gctttagata acgacgataa acgggtgaat atcaatgttc aaccgatcct
    13141 gtccccgacc ggcgatgccg ttatcggggc tctttacgtc aaaagtaata tcgaacaaaa
    13201 atattcggaa atcaataaca cagcagtcat tttctttact gcctcactaa ttgcggcatt
    13261 tatttcaatg atcgtatctg ttttagttgc tcgttcgatt acacagccga tcggtgaaat
    13321 gagagaacaa gccattcgaa tcgctagagg agactacagt cggaaagtca aagtgcatgg
    13381 acaagatgaa cttggacagc tggctgatac cttcaatcag ttagctgaac gtattgaaga
    13441 agctcaagat acaatggaag ctgaacgcaa tcgtttggat agcgtgctat cccatatgac
    13501 agacggcgtc attgcgacag atcgcagggg taaagtcatt acgatcaatg atatggctgt
    13561 ttccttgcta gatatcaaaa acgaggaagc aatcggacaa tcgattttga cacttttaga
    13621 tatcgaagaa gaatatacgt tgcggaaatt attagaagat ccaaacgaaa tggtacttga
    13681 ccgttcaacc agtacattgg aaagtgatca gatgatcttg cgagtagact tttctatgat
    13741 ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc ttgcatgatg tgacagaaca
    13801 agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg aacttcgtac
    13861 accgttgaca agtatgcgta gttatatcga agcattgagc gaaggtgcgt ggaaagacga
    13921 ggaagtagca ccaaattttt tgaaggtaac gctagatgaa acagaccgaa tgattcgaat
    13981 gatcaatgac ctcttaaatc tatctcgcat ggatacaggt aatacccagc ttcaattaga
    14041 atatgtcaat ttcaacgagt tagtgaattt tgttcttgac cggtttgata tgatggtggg
    14101 aaatcaggaa aaaaattata agatccgtcg tgaatttacg caaagagatc tttgggtcga
    14161 agtagataca gataaaatca tccaagtagt cgataatatc atgaataacg cgatcaagta
    14221 ttcaccagat gggggaacga ttacttgtcg cttattagaa acgcataata acgtgatttt
    14281 aagtatcacc gatcaaggat tggggattcc taaaaaagat ctgaaccgag tgttcgagcg
    14341 gttctatcga gtagataaag cacgtgcaag agcacaaggt gggacaggac ttggattagc
    14401 tatttctaga gaagtgatta aggcgcatcg aggcgctatt tgggcagaga gcaaggaagg
    14461 aaaagggtcg actttttata tctcgctgcc atatgaacct tatgaggagg aatggtggga
    14521 atgagaatct ctgaaaaagt cgttcgtata ggactcattt tgatggttgc gttaagcata
    14581 tacttctctt atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa cgtagatgag
    14641 acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt ctttcttccg
    14701 cttcatgtta catggcacca ttcgggaatg atccaagaaa ccaacagtga aaatctcgtg
    14761 taccgattgc aattgatcat cgataaagca agatttggaa agctttctga agtagtttca
    14821 ggagacaaat cacagtttga acaggtgaaa aatctgaact ctggttttga gttggcctat
    14881 aatgcgcctt ttttgctaag agaatacaag gaagcatttg atttagattt agatgtgtcg
    14941 aacttggcaa aagaaagcga aaatatgtat tttactagaa tacaattcga tcaaggaaat
    15001 aaaaaaatcc gatttttgaa ttattcgaac catctcgtat atgaagcaaa tatttcgttg
    15061 aattggacag atatcaataa agaattaaca gcaaaagaca caaaatggac agaaatgaca
    15121 acagaaccat cgacgatcga cacgcaatat gctacgaaac attcgatcaa actgaagaag
    15181 tatagttata tcctgtcaca gcaaccctat acacttttta gaaatgcttt tttccagaaa
    15241 ccagatagcg tgaaaaataa tgatgaaagc aatgaattga ttttttacgg tgggaatgaa
    15301 acaatgtcta tccattcgga aacgcaagta gttgattttc gtggagagct gccagagaaa
    15361 aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt aggcagttct
    15421 ttagggaacc tcagatattt tgaccgcagt gacaatacga tcgattatcg gatattcgtg
    15481 gaaggcttcc cggtttttgg gacagacagc agaggacaga ttggtgtgga agttggcggt
    15541 gaaacgaatg gttctgtgca agtgaatatc caaaccagtt tgaatacgat tcaagtgccg
    15601 ataccttcag acgaagaagt agaacttgca agtacgaatg aaatcatcca gcagttaaaa
    15661 gctaagggag cagatagcaa caagattggt tcagtgatcg ttggctatac atggcaaaat
    15721 gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa atataaagat
    15781 aactggtatt cagctaacga ccttctggaa aaattacctg gattggaggc gaattaattg
    15841 gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt tagggttgaa tgtgttttta
    15901 ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg tcattcgctc taatcagact
    15961 gaatcaatcc aacaacgtct ggctagcgac gatatcactt acacggggaa gatttcgtct
    16021 gagaacaagc aaggctatta tcttagcgcg gagcagacca atcttagcac ttcattagcg
    16081 aattatcgca aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt agacggtgat
    16141 gttttgacga aatctttatc cagttcagat gctgaaaaga acgtgatcga tccagataaa
    16201 attagtagtt ccttggaaga ttttttatct aacaaagaca atattttatt cggtaatgat
    16261 tatgtgtaca cgaaaaactt ttctcattta gaagaagaac cactagaaat ccaagcaagc
    16321 caagagtatg aaggcatccc attcaatgat gatacagcaa aaatcgttct ttctttagaa
    16381 aaaaacgata atgattatag catcacgaaa tacatgcaga cgcatttatc tgatatcgag
    16441 cagctgcgcg aaaagacgga actgtatacg gaagaggaag caatcaatac attgtacatc
    16501 aataacaaaa taccaagagg atcaaaaatt ttatggcgtc agctagctta ttcctgtatt
    16561 ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat cgaaacaagc
    16621 gataaagtgg tccaaattga gagcgtcaac gctttcagca atacgatcgt gacaaacaat
    16681 actgtaccaa aagtggaaga tcaataaaaa agcgagtata atgaaaaaag tttacaaagt
    16741 aagaagggaa gtaggagtca aatggctgaa gaaaaaccag cttttaaaat cagtatccta
    16801 gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg gcttttggtc
    16861 gatgcaggac ttagcggtaa aaaaatcact tccttgatgg ctcaaattga tcggacaccg
    16921 gaccaattag atggaatact tgtgacgcat gaacatcgag atcatataca tggcgtgggt
    16981 gtcttggctc gtaagtatca tttggatgtt tttgccaatc agcagacttg ggaagcgatg
    17041 gaaccactga ttggaaatgt accagtggaa caaaaacatc tatttgagat ggggaaagtc
    17101 agaacattcg gcgatatcga tattgagagt ttcggcgtct cccatgacgc agcggctccg
    17161 caattttatc gtttccataa agaaaatcgg tcattcgtca tgttgacgga caccggatat
    17221 tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg gttatttgat ggaaagtaat
    17281 catgatcttg agatgttacg catgggacct tatccgtgga atttaaaaca gcgtatttta
    17341 ggagatagag gacatctttc caacgaagac ggtgcacttg tcatgactga tgtgatcggt
    17401 aatcggacca aacgaatcta tcttggccat cttagcaagg aaaacaatat gaaagaactt
    17461 gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg acatgatttt
    17521 gaaatctatg atacagatcc agatgaagca actgagctat tttcgattta aacagttcaa
    17581 tcaataaaaa tataaaaagc agaagataaa atcaatcagg gttggtttta tcttctattt
    17641 ttattttttt agcatattaa aaaaataacc gtattatcga ctgactacac tgatgtgctg
    17701 cttttcatgt ttctcatcca gtatctcatc tactaatgca atcgcgtaat ctgccatgct
    17761 gatcgtactg tcgctagcag cgtttctttg taaataatca tcacttaaca aataagtacc
    17821 tgtacgtttg ccatgtggat caaataaaga agctggactg atataagtcc aatgtagatt
    17881 tttagattgt ttcaatttta acaatccttc gtacatattg aaagctgttg gatagaaggg
    17941 tgcattttta ggtacttggt caatcatacg agtcgtcttt tctgtatcta agaataacga
    18001 agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct caatcaaatg
    18061 ggacaaagaa gtttgatgca tttcttcttt tcctttggga gcgttgaaag catcaacgac
    18121 aacatcaaaa gatgcaagat cagtagttgt caactcaaat aaatcctttt gtataaacgg
    18181 aacatctgat ggcaattttt ctggatgacg cacgattgcc gtcacgtcta aatctctgga
    18241 caatgcttcg tctaaaataa atgaacctgc atggccagta gctccaatga gtgcaatttt
    18301 cataaaaaac ctcctttttt attcctgtcc attgtaagca gttgctgaat attcgtcaaa
    18361 gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat cattgggtat
    18421 agttaaatta agaaaactga ttgataaaaa tagaagttga aggagataag aaatgaaaga
    18481 gtggttagat cgtgcctttt taccaatgat aacatctgtt gtgaatctta gacccttaac
    18541 tgctttgcgt aatggaatga cttacacgat cccaataatt attgttggct cgctgttttt
    18601 cttatttgct cattttcctg tggattacat cgctcagtgg atagacaaaa caggattttc
    18661 tatgtatttt acacaagtat accaggctac atttgatgtg atagcctttt ggatcgtttt
    18721 tgccattgca tacgcttatg caaaagaaga agacatcgat cgcttaccag tcggcataac
    18781 agccttgtct gcttatctac tgcttattca gccactaaat actcagatag atactgtctg
    18841 gacaggtgct gaagggatac taggagcagt tacaactggg ctcatcatcg gatggggcta
    18901 tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga gagaagatac ctgaaaatgt
    18961 ttggcagtct tttacttcat tgattccaat cattgttata gtcactttta cgcttatcgg
    19021 ttcagcggtt tttcaacatt tgttcagaat gactgttgtt caaggagtat ggacatatgt
    19081 ccagcttccc attcaatcgt tcatagacac gttgtttggt gtgcttttat taggtttttt
    19141 tgttccgttt ttatggttat ttggtcttca tggatctgcg atggtcaatg ggttggttag
    19201 tcccgtcttg caagcgaatt cattagcaaa tgcagaaatc ttagcctcag gacaagagtt
    19261 gacggtggca aatggcggac atatcgttac tcagcaattt ctcgatcagt tcatgacggt
    19321 tacgggtgct ggattgacgt taggggctgt attttttatg acggtttttg cgaaatcgaa
    19381 gaaatacaga gaattaggaa atcttgctct tttaccagcc tttttcaaca tcaatgaatc
    19441 aattattttt tccacaccga tcgtgatgaa cccgatgatg gctgttcctt ttattttcgc
    19501 accggtcgtt tcaggattga ttacctattc cgctttatat tttggattcg ttcctttatt
    19561 tacagcagtc caagtccctt ggacaacacc gccgatcctc tcgggtttct tagtaggcgg
    19621 agtgcctgca gcgattttac aaggtatcgt gttgtcaatt agttttttca tttatttccc
    19681 atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa agacagacaa aaaatgatgg
    19741 agcagcagaa aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt agattgggaa
    19801 aatcaccaat ctaggatgac aggagtattt atagtcatgt ttatactagc cctttttgga
    19861 cagctaattg aacaagaaca actaatgtgt tcattcctac gtgtgtgatc atcgatgtcc
    19921 agatccgacc agttgctttg tactctaggc tgaagaagaa acctaggaaa aagtagatta
    19981 gcagatgacc atcattatga gcaaacgcaa acagcaaaga gctgatcact gctccgaccc
    20041 aaaaatttga atatcgactg atgatcccca gtatactgcg gcggaatacg aactcttcca
    20101 tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg atcatctgga
    20161 tgatattttg tgtattctcg gaaggggttg ctttgccaaa caatacagat tctaacatga
    20221 cagcgatgct ttgcagaatc aatgcgacaa agattccaac caatccataa ataacaatct
    20281 ttggagcaga agcttttttt accgctgatt cgatcgctaa gggttcacgt tgcttaaaat
    20341 aaaaaatagc taggacaacg gcgcctagta tatacatgac tgcagttgct gtgatcaatt
    20401 cagacgtaga ttttacaagt cctatcgaag ctaggatcaa gggtgagata aaaattagac
    20461 catagcaaaa aatgctgagg aagctatatt tttttaaaga catgtaattc ctccttttaa
    20521 gggataacaa gcatgcaagg caagcttata tcgattgatt tcaattatat cataatttca
    20581 ataagcagct gtcagaataa aatttctttt ctttgattta aaagcttgca aaaaagaggg
    20641 agaatgagta ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc taacagatta
    20701 aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat taggtgatcg cgtcattatc
    20761 gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc tgctgctaaa
    20821 gagaaacccc aaactgggac agttgttgca gtcggtgaag gccgtttgtt agaaaacggt
    20881 gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa atacgccggt
    20941 acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga tatcatggcg
    21001 attgttgagt aaatttggaa gataaaaaaa taatcaaaaa taatcaatga ggtgaatcaa
    21061 tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc agctatgtta cgcggggtag
    21121 ataaattagc agatacagtg aaagtaacat taggacctaa aggccgaaat gtcgtactag
    21181 aaaaatctta cggctctcca ttgatcacaa atgatggtgt gacgatcgca aaagaaatcg
    21241 aattagaaga tcattttgaa aacatgggtg caaaacttgt atcagaagta gcatcaaaaa
    21301 caaatgatat tgcaggagac ggtacaacga cagcaactgt tctgacacaa gcaatcgttc
    21361 gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct aggcattcgt cgcgggatcg
    21421 aattggcaac aaaagcagca gtcgaagaat tgcataatat ctcaactgtt gttgattcaa
    21481 aagaagccat tgcacaagta gcagctgttt cttcgggttc tgataaagtt ggacatttga
    21541 ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa gaatcaaaag
    21601 ggatcgaaac agaactagat gttgtagaag gtatgcagtt tgatcgtggt tacttatcac
    21661 aatatatggt aacagataac gacaaaatgg aagctgttct agaaaaccca tatatcttga
    21721 tcacagacaa aaaaatctct aacatccaag atattctgcc attgttagaa caaatcttgc
    21781 aacaatcacg tccattgttg attattgctg acgatgtaga tggtgaagca ttgccaactc
    21841 ttgtattgaa caaaatccgt ggaacattca acgtagtagc tgtaaaagct cctggtttcg
    21901 gcgatcgtcg taaagcaatg cttgaagata tcgctatttt gacaggtggt acagtaatta
    21961 ctgacgatct aggtttagaa ttgaaagatg caacaatcga aaacttagga aatgcttcta
    22021 aagttgttgt agataaagac aatacaacga tcgtagaagg ttctggagaa aaagaagcaa
    22081 tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga aacaacttct gattttgacc
    22141 gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt gttaaagttg
    22201 gtgcagctac tgaaacagaa ttgaaagaat taaaattacg cattgaagat gcattgaatg
    22261 ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct ctagtgaacg
    22321 ttatcagcaa agtttctgct gtagaagcag aaggagacgt ggcaacaggt atcaagatcg
    22381 ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga aaacgctggt tatgaaggat
    22441 cagtgattgt tgataaattg aagaacgtgg aactaggcac tggtttcaat gctgctactg
    22501 gagaatgggt aaacatggtt gaagcaggta tcgtagaccc aactaaagta acacgttctg
    22561 ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac gacagaagca gtcgttgcag
    22621 ataaaccaga accagcagcg ccagcagctc ctgcaatgga tccatcaatg atgggcggta
    22681 tgatgtaaga aaaagcaact tgcttatcta caactgatca ttagtgaaag acagataaaa
    22741 agaggctgga acagaagcgt ttaactccaa gaaataagaa gaaattcacg gaaattgctt
    22801 ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt ccgctgttta
    22861 tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtctca
//
LOCUS       DAYYBH010000043        22719 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000043 DAYYBH010000000
VERSION     DAYYBH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.043"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACHAUG_001806"
     CDS             complement(11..1942)
                     /locus_tag="ACHAUG_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8480896.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACHAUG_001807"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACHAUG_001807"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8480897.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACHAUG_001808"
     CDS             complement(2871..3623)
                     /locus_tag="ACHAUG_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8480898.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACHAUG_001809"
     CDS             complement(3782..3991)
                     /locus_tag="ACHAUG_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480899.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACHAUG_001810"
     CDS             complement(4002..5330)
                     /locus_tag="ACHAUG_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480900.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACHAUG_001811"
     CDS             5466..5621
                     /locus_tag="ACHAUG_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8480901.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACHAUG_001812"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACHAUG_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8480902.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACHAUG_001813"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACHAUG_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8480903.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACHAUG_001814"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACHAUG_001814"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8480904.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACHAUG_001815"
     CDS             complement(9305..10237)
                     /locus_tag="ACHAUG_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480905.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACHAUG_001816"
     CDS             complement(10394..11053)
                     /locus_tag="ACHAUG_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8480906.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACHAUG_001817"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACHAUG_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8480907.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACHAUG_001818"
     CDS             complement(12094..12384)
                     /locus_tag="ACHAUG_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480908.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACHAUG_001819"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACHAUG_001819"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8480909.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACHAUG_001820"
     CDS             complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACHAUG_001820"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8480910.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="ACHAUG_001821"
     CDS             complement(16049..17218)
                     /locus_tag="ACHAUG_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8480911.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACHAUG_001822"
     CDS             complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACHAUG_001822"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8480912.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACHAUG_001823"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACHAUG_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8480913.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="ACHAUG_001824"
     CDS             22182..22703
                     /locus_tag="ACHAUG_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8480914.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6326 a         4932 c         4041 g         7420 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata aggagcgtg
//
LOCUS       DAYYBH010000044        22229 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000044 DAYYBH010000000
VERSION     DAYYBH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.045"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            299..2134
                     /gene="tdc"
                     /locus_tag="ACHAUG_001825"
     CDS             299..2134
                     /gene="tdc"
                     /locus_tag="ACHAUG_001825"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8480915.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2439..3917
                     /locus_tag="ACHAUG_001826"
     CDS             2439..3917
                     /locus_tag="ACHAUG_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8480916.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3883..5214
                     /locus_tag="ACHAUG_001827"
     CDS             3883..5214
                     /locus_tag="ACHAUG_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8480917.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5328..7880
                     /locus_tag="ACHAUG_001828"
     CDS             5328..7880
                     /locus_tag="ACHAUG_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8480918.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7887..9326)
                     /locus_tag="ACHAUG_001829"
     CDS             complement(7887..9326)
                     /locus_tag="ACHAUG_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480919.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9301..9483
                     /locus_tag="ACHAUG_001830"
     CDS             9301..9483
                     /locus_tag="ACHAUG_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480920.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9672..10034
                     /locus_tag="ACHAUG_001831"
     CDS             9672..10034
                     /locus_tag="ACHAUG_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8480921.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10221..11078)
                     /locus_tag="ACHAUG_001832"
     CDS             complement(10221..11078)
                     /locus_tag="ACHAUG_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8480922.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11198..12109
                     /locus_tag="ACHAUG_001833"
     CDS             11198..12109
                     /locus_tag="ACHAUG_001833"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8480923.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12116..13762
                     /gene="mdcA"
                     /locus_tag="ACHAUG_001834"
     CDS             12116..13762
                     /gene="mdcA"
                     /locus_tag="ACHAUG_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM8480924.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13864..14163
                     /locus_tag="ACHAUG_001835"
     CDS             13864..14163
                     /locus_tag="ACHAUG_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8480925.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14153..15829
                     /gene="mdcD"
                     /locus_tag="ACHAUG_001836"
     CDS             14153..15829
                     /gene="mdcD"
                     /locus_tag="ACHAUG_001836"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8480926.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15833..16426
                     /locus_tag="ACHAUG_001837"
     CDS             15833..16426
                     /locus_tag="ACHAUG_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8480927.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16450..17526
                     /locus_tag="ACHAUG_001838"
     CDS             16450..17526
                     /locus_tag="ACHAUG_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8480928.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17551..18420
                     /gene="mdcB"
                     /locus_tag="ACHAUG_001839"
     CDS             17551..18420
                     /gene="mdcB"
                     /locus_tag="ACHAUG_001839"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8480929.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18531..19295
                     /locus_tag="ACHAUG_001840"
     CDS             18531..19295
                     /locus_tag="ACHAUG_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8480930.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19784..21877
                     /locus_tag="ACHAUG_001841"
     CDS             19784..21877
                     /locus_tag="ACHAUG_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8480931.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21902..22204
                     /locus_tag="ACHAUG_001842"
     CDS             21902..22204
                     /locus_tag="ACHAUG_001842"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8480932.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7204 a         3790 c         4731 g         6504 t
ORIGIN      
        1 tttttaacaa tataaaaaaa taaaaatatg tttagtgaat gaaggacagt cattttattt
       61 gtttgttcgc tttttttagg ttatctattt tagaagactg atttattgaa aaattgttcg
      121 cttttttgcg ttgaattatc agaaaaatcc gactttattt atttttgttt gtaaaaatct
      181 acgtggattt gatgttataa aaaaaaaatt ctcatttttg gcgctatagt gaagaggtgt
      241 tacagataaa cacatgtatc tgcaagacat tgaccaacaa aatatggagg gaagaaaaat
      301 gaaagatatg gatatcaagg ccgtctttat aggcgacaaa gctgaaaatg gaccagttta
      361 taaaatgttg ttgaacaaga tggtagatga acatcttgga tggagagaaa attatattcc
      421 ttcagatatg cctgcaatta gcgaaggaga taagctcact ccagattatc tagccacccg
      481 cgaccatatg atagaggtct tggatgaagt cagtcagcga ttacgcgcag gatcgatccc
      541 atggcattca gcaggccgtt actggggaca aatgaatgct gaaacattga tgcctgcttt
      601 attagcttac aactatgcga tgctgtggaa cccaaataat gttgcattgg aatcttctat
      661 ggcaacttcg caaatggaag cagaagtagg acaggatttt gcttcattat tcaatatgac
      721 agacggatgg ggacacattg cagcagatgg ttctattgct aatcttgaag gattatggta
      781 cgcccgttgc atcaaatcga ttcctttagc tgtcaaggaa gtgctgcctg aaaaagtgaa
      841 aaaaatgtct gagtgggaac ttttgaattt atctgttgaa gaaattttgg aaatgaccga
      901 aagctttacg gacgaagaaa tggatgaggt caaagcagct tcttcccgta gcggaaaaaa
      961 tatccaaaga ttagggaaat ggctggtccc tcaaacaaaa cactattctt ggatgaaggc
     1021 gttagatatt tgcggagtcg gcttagatca gatggttgct atcccagttc aagaggatta
     1081 tcgaatggat attaatgctt tggaaaaaac aattcgtgaa ttagctggac aaaaaatccc
     1141 aattctaggt gtggttgctg tcgtaggaac gacagaagaa ggtcaagtag acagtgtaga
     1201 taaaatcgtc caattaagag aaaggttaaa agatgaaggg atctatttct atttacatgt
     1261 tgatgccgca tatggcggct atgcgcgttc actgtttcta aacgaagcag gagaatttgt
     1321 gccgtatgct tccttagctg aattctttga agaacatcat gttttccacc actgcgtaac
     1381 gattgacaaa gaggtatatg aagggttcag agctatttca gaagcggatt ctgtcacgat
     1441 agatcctcat aagatgggct atgtccctta tgctgctgga ggaatcgtaa tcaaacataa
     1501 aaatatgaga aatatcattt cttatttcgc gccatatgtg tttgaaaaat cagtaaaggc
     1561 tccagatatg ttaggagctt atattttgga aggatcaaaa gctggagcaa ccgccgccgc
     1621 agtctggaca gcacatcgtg tattgccatt gaatgtgacc ggatatggtc aattgattgg
     1681 ggcttcaatc gaggcagctc aaagattcag agaatttctc gatcatctga cttttactgt
     1741 taaaggaaaa acaatcgaag tctatccatt gaatcatcct gattttaata tggtcaattg
     1801 ggtattcaaa gaacaaggct gtacagattt gaacgctatc aatgaattaa atgaaaaaat
     1861 gttcgatcgg tcttcttata tggatggcga tgtttatggt gaacggttta ttacttctca
     1921 tactacattt acacaagaag attacggtga ttctccgatt cgttttgttg aaagaatggg
     1981 tctaacgaaa gaagaatgga aaaaagaaca gaaaatcact ctgctacgtg cggctatcat
     2041 gactccttat ttgaatgatg atcgaatttt caatttttat acaaaaaaaa tcgccaaagc
     2101 aatggaaaag aaactaaacg aaatcatcca atagaactga tttttgtgtg aaagaataac
     2161 aagagacata tggagaacag acaattattt gcttcatcaa aacaattgta tttccccctg
     2221 tttttcatat attcctcgcc aagatattct tttttataaa acgttctata agttggatcg
     2281 ttcgtttcat gtatcaaacc cagttcacta agatcgttca atggatgttt cggtgatact
     2341 gggttttgta ctgaatcgat gaaaccttgt catttctgtt cttgaacaaa aataaaagaa
     2401 aacaaaaaca tatgtcgcta ttgaaagaag gaataaagat gaaagatact ggttcatcaa
     2461 agcaaatcac gaccatgacc tttatcggaa tgacctgcgc ccttgtagca agtgtacgaa
     2521 atattccaga tgtagcagca acgagctgga cgatgatttt ttacatgctg gttgcagtat
     2581 tattgtacgc ttttccaatc tcattgatct ctggtgaatt tgctggaatg tttccccaaa
     2641 aaggtggtcc agaactttgg gtatcgaatg ctctaggaaa aaaatggggg ttcgttgtct
     2701 catggctgtt atgggtccaa atgtttcctg ggatggtgat ggtcgcttca gcactcgctc
     2761 cgttgtttgg taatattatc gataatgtgc cgcttggttt gaacagcaaa tttacattag
     2821 ttgtcatttt agtcgtttat tggatcatca cttttttgaa tttgaaattt gatatggcaa
     2881 aaatcggcgg aaaagtcggt gtttggttag gattgtacgt gcctttgacg atgatgctct
     2941 tattagggtt tgctgcgtgg atcaaagttg ggatcgtacc aaccggaaca ttaggcagtt
     3001 tttcatggga caaattaata ccagatacta cgactgcatc ctcatttgtt tattttgcgc
     3061 cgatcatgtt tatttttact ggtatcgaaa tgtcctctgt gtatatcact cgattggaaa
     3121 atccagttaa aacgtatatt cgcggagtat ttgccgcgtt aatttttata tttttcgtca
     3181 atattttgaa tgctttagtt gtagcaaatg tcgtaccaaa aggacaaatg gaattgaata
     3241 atatcgcgca atctatttcg atttattgcc aaattcttgg tttgccacat cttattgtga
     3301 atctgtttag tttacttgtc ttcattggtg tagcagtcca attatctgct tgggcctcag
     3361 gaccagcaaa gacagtgaca gaaagtgcta gaaaaggtgc gtatccacca aaattcaatt
     3421 tttggaagac gaatcaattt gacgtatcca aatccgttat tttgactcag tctgtcatta
     3481 tctcaatctt tgcacttttt tatttgctga ttcctggcgt caatcaagca tttttgatgc
     3541 tcgtaaattc aactactgtt atctactgta tcgtctatgt catcatgggg atcgcagttt
     3601 tgagattgcg ttatacacat gcaaagttga atcgaccatt tagaattgga aaaaaagaga
     3661 aaaagagcaa tcttggagtt tggactgtcg ttattgtttt atttgcggca atcggatttt
     3721 ctgtcggact gacgatgaaa gctggaacat ggatcaatct aattgcagtc acagccatca
     3781 gtgtcatttt gtttgtagta ccactctgta ttgaaaaaat aaagaaacct tcttgggaac
     3841 aggaagtcgc catgctgctt gaaaaagaaa aaggtgaact tgatggaaaa accggcaatg
     3901 aaacggactc taactaatcg aaatatccag ttaatcgcat taggtggcgc aattggaaca
     3961 ggattatttt taggttcagc aaaggcaatt gcatcagcag ggccagctgt tattctcgtt
     4021 tatttgatat gcggtttggc tatttatggt ttgatgcggg ccatgggtga tttattttta
     4081 gcaaatagta catttaactc tatcagtgat ttcgttgact attatttagg caaaaagtgg
     4141 gcattttttg tcaattggac ttattggctt tgctggcttg gagctggact tgcggaatta
     4201 acagcaatag gattgtatat ccatttctgg tttgtacatg taccagcttt gataagcgga
     4261 ggtagcgcac taatgatctt aatggcagtc aaccttgttg cagtcaagtg gtttggtgaa
     4321 ttggaatctt ttttctcaat catcaagatc ttggggattt tcctttttat cttattgggt
     4381 ataggattga gtgtctcaga ttttcatacc cagtctatcg gaatcaaaga aaattttgga
     4441 cagatcgatg aattactacc gtttggcttt gctggaatgg tagtctcatt tcctatggtt
     4501 ttattcagtt ttgctggaat tgaaatgatc ggcttaaccg ttggcgagac aagtgatccg
     4561 caaaagaatt tgaaaaaagc aatcaactgt ctgccttggc gtattctctt tttctatata
     4621 ggaacgattt tgtcgatttt atttgttgtt ccatggcatt gtttagattt aaaagaaagt
     4681 cctttagtga ctatgctgca agtgatccat tgccaaggcg gagcagcaat tgtcaatcta
     4741 atgattttgg ttgcttcttt gtcctcagca aatagtgcag tctacagtac tagcaggatt
     4801 ttatatcaaa cagcaaaaga aaaaagtttg ccccattggt tttgtgcgct ttctaagaaa
     4861 ggcgtacctt tacacggtat tttaattaca ggaatgttat tttttgccgg tctttgtcta
     4921 catttttttg tagctgatag tcgattgttt tttcagcttt tggcaggcat gacaacgagt
     4981 tgttttctat ttgtgtggtc ttcgatcttg tgtgcacatg ttcaatttat taaagaaaag
     5041 aaacggaaca ctacacaaag atataaagat cttgctttgt tggtgttttt tacttcaatt
     5101 gctttcagct tgttattcga aagcacgaca cgctttattc cttttttatt ggttatctgg
     5161 tttgtattct taaattttgt atataaaaga ataagcaata gaaatatcag ttaggcccag
     5221 atacattttg caaaacgaat tatttcagac ccccctgaaa taatcgatac gcctgaaaaa
     5281 acttccctgc gtataataac agtatcaatt aaacaggagg ggataggatg aactggtaca
     5341 gattgacggt agaagatgta ttgaaaaact atcaaacatc aacagaaggc ttgtcgcaag
     5401 aagaacgaca gaaacgcctg actgatcaag gatataatga aataaaagca aggcagcaga
     5461 caaaacgatg gaaaaaaata gcaaagcatt ttactgatct gctgatgatc gtgttgatca
     5521 ttgcctcttt tttgaaattt gcttctagtg aatatattga aggaagtatt attttattcg
     5581 tagtgatcgt caatggactc gttagttact ggcaagaaag aaaagcggaa gaatcattga
     5641 atggtttgaa gcagctgatg ggacaagaag cgatagtact atcaaacggg atgcaagaaa
     5701 aaatccctgc gaaagaactg gtaaaaggag atgtcattgt cttacgtgcc ggagatgtcg
     5761 ttccagcaga tatccgatta atcgaagcac atgatttatt agtggaagaa tcgattttga
     5821 cgggagaatc agaagcatct gaaaaaagtc atgccgtttt gacagaagaa gaatcgatcg
     5881 gcgaccagaa aaatatgggt ttttcaggaa cgcttgtaca atctggttct gcagtcggtg
     5941 tcgttgtaga aacaggaaac acgacagaaa tcggtaaaat caatcaagca ttgcaatcgg
     6001 tcgaacaaca aacgacgcct ctgattaaaa aaatcaaccg attaaacaaa caaatttttc
     6061 aaggcattct ttgtttgagc ctattcttag ttatttttac gacatttcga tatggtatgg
     6121 attggcatat ccttctttcg gctacgattg ctttggtggt gtcaatggta cccgaaggat
     6181 tgccagctgt tttgaccatg attctatccg ttggagtaca tgaaatggca aaagaaaagg
     6241 cgatcatcaa aggacttcct tcggtagaaa cattaggttc tatgacagtg atctgttcag
     6301 ataagacagg aacattgaca aaaaatgaaa tgacagttgt ggatgtagct gcaaaagaag
     6361 aagcctgcgt gttgtccatt atgaaaaact gtcaggaatt aaaaacaaaa gaagaacaga
     6421 aaacagaaaa tttaagtggc aatccgacag aagttgcgct gattcgacac acagaaacag
     6481 ccaatctacc tttgaaaaaa atagaagcaa agattccgtt cagttctgaa tacaaatata
     6541 tggcaacgat gcatgcggag gaaaaaggag cggtgattta cgtcaaaggt gcaccagaag
     6601 tattatttgc taaatcaact ctttcttctg cagaacaaga agcatggagt cagacagctg
     6661 cagaatttgc gagaaaaggt cagcgtgttt taggatttgc ttataaaaaa gtagattcga
     6721 aacaggaact cacgcatgag actttaaccc aattaacttt tgcagggatc gccggattga
     6781 tcgatccgcc aaaagaaagt gcagtcaaag ctgtaaagga atgccaacaa gcagggatct
     6841 cagtgaaaat gattacaggg gatcacaaag acacagcaaa agcgattgcg gaacaaattg
     6901 gcttaaaaca tactgccaaa gttttagaag ggatcgattt ggatcttatg tctgatgaag
     6961 agttgattca gcaagtgcca atagtcgatg tctttgctcg aacgacacca gaacataaac
     7021 tgcgaattgt taaagctttg cagaaaaatg gagaaatcgt cggaatgacc ggagacggcg
     7081 taaatgatgc acctgctttg aaaagatccg atgtaggaat tgctatggga atcaaaggta
     7141 gtgaagtaag caaacaggca gctgatatgg ttttaggaga tgataatttc catacgattg
     7201 caaaagccgt aaaagagggc cggcggattt tggataattt gcaaaaaacc atcaatttct
     7261 tcttgccaac tgcattagct caaggattga tcttgatttg ggcacttatg ctgaatcgcc
     7321 cgctaccttt gtccccagta cagattttat gggtcaatat ggtaacgaca atcactttat
     7381 cttatgcact agggttcgag tcgccagatc cagaaatcat gaagcgccca ccaagagatc
     7441 caaagcaagg aatcctatct ggttatcatt tgtttcggat catctatgtc tccctactga
     7501 tcatggttcc tgcctattta ttggctatgc agtttgaagg gcaaagctta cagcaaacca
     7561 ttcttctgca aaatatcgtc ttagctcaag ctgtttacat gatcaattgc agagagttgt
     7621 ccaagccgtc attgaaccaa ggtcttttgc aaaacaaagc gctttttctt tcattaggta
     7681 tccttgcttt attgcaaggg atggttattt ttctaccagt cggccagcag ctgatcggta
     7741 cagtaagttt aacgatgaca caacagttat tgattggagc aaatgtgatt cttctattct
     7801 tagttgtaga aatagaaaaa gggatcatga acaaactgtt tcgcaaaaaa gaaaaaacag
     7861 ctagagaata cagtcgctag ccgttactat aaaaaagttt tgaaagtgag ttcagtcaaa
     7921 cgattgagct catttttcgt tggaatagaa gaaatgtata caatagggat atctagagaa
     7981 tctagaggga aattggaaat caagatatct gttttttcaa aagaaatagt ggaaagatcg
     8041 gttagttcga taggtaagaa gcagacacgt tttcctaagt aatcttttaa ttcctgctgc
     8101 aagaacgcct tccatgaagg ttctccttgg aaaataaaat agacattgaa caaattcggt
     8161 ttgatagaaa aaatcgtttg ttggatcagt agagacaaga gttggagaaa gtaatttggt
     8221 tcattgattt ctatttcttt ttttagatcg ttcagtaaat tctctttctg ttgatacaag
     8281 tgtgcatatt gctgatcata tgtttctaaa aaacgatggt attgacggtc caatgcttca
     8341 atcaatatcg gcgattcatg atattttaat atgaggagag ccaagttatc agtcagagat
     8401 gtttttttta gttgatcaag agacagctct gaagctagtt cctctacaaa cgtttcgagg
     8461 acctctaaaa atggagaatg atgcaggcag cgagcggttt cttgtccata gttattgttg
     8521 taattccaac tttccagaaa gcagaaagaa gcaaaagaaa gctcagtgtc tttaagataa
     8581 ataccttccg ttaaatacaa ttttttcaat gcagagtcat acagggagga gagtgtgctg
     8641 ttggtatgga tagaagaatc taatcggcaa ttcgctttta tacggatcgt attgataaag
     8701 aaccaacatg ttccgaaaat ctcttctgtt tcaaccgcta gttcagtttg actgaggttt
     8761 cttaaaaatt gaaaataatg ggattcatgg atcgaataat cctcgaaaaa atagttgtta
     8821 tgagtgaaag gaatcagcaa tcgatgataa aaagtacgaa tcgcacgctc ttttccaaga
     8881 atacaagctg tatatttcgt caaatcgatc tgcaagtcgt aacttgctaa acgctgattg
     8941 attttagtca catgtctttt caatgtccca taagaaagcc catggtcggc tagaaaatca
     9001 gggacagcaa ctttttttgt aaaaagcaga tttttcagca gttcgacact gactgattgt
     9061 ttgaaaaaaa tctcccaaag gtcatttgtt aataaatttt gctggttttg caaactgatt
     9121 cctgcttgat cgctgatgat gtcccagcct tcgggcagct gagagcgaat agattgaagg
     9181 tcagaaaaaa tcgttcgttc ggttgtccct atgatttcag ctaattcatt tacggttatc
     9241 tttgtatgat tcagcaattg ttctactaaa gaaacatgac gtcgaatatc tttttcggta
     9301 atgatttttt tcattagtga atacatcaat cagactccct tctattctaa gaatacggag
     9361 aaaataaacg gtggtcaagt ttcaactgat ttttcaatca ttgtttattt ttctggagca
     9421 tttactgatg ataagaattt catcatgaac ttgaaaaatg ataccactaa aatcaaaaaa
     9481 taggtgggca aacaataata aacattttaa tagtagatgg tagcaagacc aacacaggcg
     9541 gattgaacat ttaagagttg ataaaaaaga aaaatatcga agtatttgat tagcaactac
     9601 ttgtattcat ttttctattc ttacgtggta tctttgaata aagcgaatca ataagaaaga
     9661 gatggaagca gatgctaacg aaaaaagaac taatcgatca catcaatgaa acatacaatt
     9721 tgtctccaga atatattttt aaaaaatttc ctgattattg cgtattcaga catcaaaaaa
     9781 gcaaaaaatg gtttggggtc ctgatgtctg ttccagaaga aaaaatctat gatgatgcaa
     9841 gtgaccatag gatcatcaat attcttgatt tgaaggtcga tcctgaactt ggcggaattt
     9901 tgcgaggaaa agaggggtat tttccagcct atcacatgaa taaagaacat tggatctcta
     9961 ttgatctacg taaaatagaa aagctagatg aactagcaga tttgattgat ggaagtttta
    10021 aatcaacgca atgaatgtct aggacaatag agaagaggtt gtgacagaaa tgtttagctt
    10081 caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgggaaa tttcggctta
    10141 ttttctaaga aattacttct ggaacaccgt ttgttcgaaa aaagagctgc gacaagaatt
    10201 ttgtcccagc tctttttttt ttattttcct aaataaaatt cccgcatcgt ttttgcaatc
    10261 attttgatca aaggatgatc ttctgtgaag ctagaagata aataaattgt tcgtgtcaag
    10321 ggcaaatctt tgatttcccg caagacgaaa ggatcatttt tatcgtaaga ccaagaaact
    10381 tttgggaaaa agctaacagc taatccttgc ttgacaatag aacgtaatgt cgcaggatca
    10441 tctgtcgtcg aactgatcgt caattcgata cttttcgctt caagtgctgc atccagtgta
    10501 tgacggaagg cgttgctctt tgacagcatc aagatggggg tgcgttggat atcttctaat
    10561 tgcagatgtt cttttttagc aagaggatgt ttcttaggaa tggcgacata aacaggttct
    10621 ttcatgatag gaatattggt catgccaggc ttcatctccg aagtgatgta cagtacagga
    10681 tcggtaatat catctggtaa atgatgttgc atgatcgtta attggatatc aggatgcttt
    10741 tccataaaaa ttcggaccaa gttcggaata gaaacagagg ctacctctaa atacaatatg
    10801 acggaagtaa cgatcttctc attgaccgaa gacaataatt tgattccttc ttctaatgtg
    10861 tctaatgtct tgactgcata tggataaaat tcttctgcat aaacggtcgt ctcaatgttt
    10921 ctgccgctat ggtaaaacaa agggacacct aattcgtttt ccaaatgttt gatcgcacgg
    10981 ctcaaggcag gttgagaaat aaataattct ttggctgctt gggtaacatt ttttgtttcg
    11041 aatacttttt taaaatactg cagatgaagc aatttcataa ttatccatcc catccatacc
    11101 tttttgttat ggttttttct ttttttggca ttttttatta tagcagaaat tgactaagat
    11161 agcagtggaa aaagtaagtg ctgaggaggc gaggctcatg agtacaggtt tcttatttcc
    11221 tggacaaggc agtcagaagt tagggatgtt agcggatatc cctgaaatgt acatggaacg
    11281 tttagaaaat gtgacggggt atcggttagt tgataaagaa gaaaactatc aagatacggt
    11341 ttttatccag ctagcattac tgacaaaagc agtattttat ttagatgaaa tggaaaaaaa
    11401 cggtattcag cctgatattg cagcaggaca ttcgattggt gctttttgtg cagctgttgc
    11461 ttgtcgatct ttgagatttg agaatgcagc ggaactagtc tatcaccgcg cagtcttaat
    11521 gaaagaagct tatccaagcg gttatgcaat gggcgtagta gtaggtgtga cgagaagcga
    11581 agctgaggaa attgtagcaa aaacatttga tcctgaccat ccagtttatc tgtcaaatga
    11641 aaattgtccg atgcaacata cgttatctgg gagtatcgaa ggtttatccg ttacgttggc
    11701 agaagccaaa cggcaccaag cgcaaacagc gaagctgctg aaagtccctg tgccatctca
    11761 ctgtctcttg atgaatgata cggtcaagaa attcaagccg tatatgcagt ctgtccatct
    11821 atctgatgct agatgtttgt atataaaaaa tacggatgga agagccactc aggacgcaga
    11881 agagattcgt aaagatttgt tggaaaacct agctttccct gtgcagtgga accaaatgat
    11941 ggacttggca aaagaattag gaatggatct gacgattgaa tttccccccg gcaatacttt
    12001 gacaaagctg attcatacaa aatttggtgt agataaaggg atccgtacga tcaatctaga
    12061 ccaacatggt attgatgacg ccacgtttct atatcaaaaa tggagatgac taattatgga
    12121 aaaaccaaac cgcaactggg cttctaaacg cgaaaaaaaa gcgcagcgat tggaaaaaat
    12181 cagcaacttg ctcaatggca agtatgtaga aactgaaaaa atcgtagaag taatggaaaa
    12241 actgattgct cccggcgaca aagttgtatt agaaggcgac aatcaaaaac aagcaagttt
    12301 tctttcagaa agtcttgaaa aagtagatgc agaaaaagtg aatgatctgc atttgatcat
    12361 gtctagtatc tcacgaccag aacatttgaa tatttttgaa aaaggaatcg caagcaagat
    12421 cgatttctct tatgctggtg cacaaagctt gcgtgtcgcc caaatgattg aagacggcac
    12481 aatgaaatta ggtgatatcc atacttattt ggaactttat ggacgcttat tcatcgattt
    12541 gattccaaat gttgttttag tagctgcaga taaagcagat aaagacggca acttgtacac
    12601 aggtttcaat acggaagaaa caccaacaat catcgaagct gcagctttca aagatggtat
    12661 cgtgatcgtt caagttaacg aagttgtcga ttctttacca cgtgtagata tccctggtga
    12721 ctgggtagac gttgtcgtga aagcagataa accttatcaa ttagaagctt tattcacacg
    12781 agatcctcaa aacatcacag aattgcaaat tctaatggca atgatggcaa tcaaaggaat
    12841 ctacgcagaa cacggcgtac aatctttgaa tcacggtatt ggattcaata cagcagctat
    12901 cgaattgtta ctgccaactt atggtgaatc actagggtta aaagggaaaa tcgcgaagaa
    12961 ctgggtattg aacccacatc caacgatgat tccagccatc gaatcaggtt gggttgaaag
    13021 catccatagt tttggtggcg aagttggtat ggaaaaatat attgctgctc gcccagatgt
    13081 cttttttgtc ggtaaagacg gcacgatgcg cagtaaccga acattatcac aagctgcagg
    13141 acaatatgcc atcgatatgt ttatcggttc aaccttgcaa ttagattacg aaggaaactc
    13201 ttctaccgta acaaaaggac gtttatctgg ttttggcggt gcgcctaaca tgggacataa
    13261 tcctggcggt cgtcgtcatt ctacaccagc atggcaatca cttcgcgaca atgatgatcc
    13321 attagggaaa ggccaaaaat tagttgttca aatggttgaa acatatggtt caaataaaaa
    13381 accagtcttt gtcgaagaat tagatgcact agctgttcaa aaacaagcag gcttagccaa
    13441 tgcaccagtg atgatttata gcgaagatac tacacatatc gtgacagaag aaggaattgc
    13501 ttatttgtac aaagcaaaaa caaaagaaga acgacaagca gcaattgcag caattggcgg
    13561 tgttacacct cttggaatga ctagcacgaa agaatcacta gatagcttgc gtgcagccgg
    13621 catcgtgaaa ttaccagaag atctaggcat caaacgtagt gaagcgaaac gttcattatt
    13681 agctgcacaa accatcgatg atttgatcga atggtcagat ggcctgtatg aaccaccaat
    13741 gaaattcaga acttggtcat aaaattggcg agaaaaacct tttgtcgcca tttacccaga
    13801 tggaagaaga aagacgaata agcagaacgt gtatgctctg ttttacaaaa aggggagaga
    13861 tccatggaac aattagctta taaatatcca acgaaacagc cgatcaataa gaaaatccat
    13921 gtaggagtgg tcggttcagg agatttggaa atcttgatgt ttccaacaga aaaaacagaa
    13981 tctactgtaa aaatttgtac aggaagcgat ggcttcggcg aagtatggaa caatgtatta
    14041 acgagatttt ttgatcgtta tccaatttca gcagatatcg tgatcaatga cttcggtgct
    14101 acaccaggag ttgtgtacct acgattgacg caagcaatgg aggaactttc agatgaaaaa
    14161 tagttttgta gaattaaacg ccagagaacg agtgaacgct ctacttgaca atggacgtga
    14221 attgattgat ccattcgatc aaatcatcgc gccaagttta ccagcacaag gaatcgtccc
    14281 tgaaagtgat gacgggatcg ttgtcagccg tggcacgatc aatggcaaac aagcagtagt
    14341 aatcgctatg gaaggaaaat tccaaggtgg cggtatcggt gaagtttccg gtgcaaaaat
    14401 cgttgctgca cttgaacatg tgttagaaga aaataaaaaa ggaaatgaga tctttccagt
    14461 cattgtacta gatacaggtg gtgttcgttt gcaagaagca aactacggac tgttatctat
    14521 ttcagaaatc ggaaatgtaa tcattgctct aaaacaatat gtaccagtca tcggtttgat
    14581 tccaggacgt gtaggatcat ttggcggtat gtcgattact tcagcattaa tgagttattt
    14641 aatcgcaaca aaaaaagcga gagtcggttt gaatggacca gaagttattg aacaagaagc
    14701 aggcgttcga gaatttgatt ctagtgacaa agacttgatt tggaatacat taggatctcg
    14761 tcaacgggta gaatcaaaaa tcattgatga attagtgact gatagtgtag aagcaatcaa
    14821 agctgctgtc atcgcagcaa tggacgaacg aaaagacacg catcgcagtg aaagctcagc
    14881 attttattta tcattacttg attctttaga tctttcaaaa ccaatggcca ttgacgatta
    14941 caatgaagca attgctaacc atcaagcaaa atcaattgac ttaccagaag tagcagaagg
    15001 aaatgaacca gctgcagcaa gtgttggtta cgattggttt gcggcactaa ctggcatgaa
    15061 aaatccaact tcttctatta gtactgtgta ttatggtaaa tcaagtaaat tcatagaaga
    15121 agccatcgtg acatcaatcg tacctgatcc taaaaacccg atgtaccgtg tacgaaacgg
    15181 tgaagttggt ttagttgaag gtttccgcat gggacaaatt ttggatcacg tttacgaaga
    15241 agataaagac aaaaaagtga aacggccaat catcgttgtg atcgatgtac caagccaagc
    15301 atacggctat aacgaagaat tgatcggtat ccatgtggct ttggctaata gtgctgctgc
    15361 ttatgcaaaa ctccgtcaag ctggacatcc agttattgga ttgattgcag gaaatgctat
    15421 ttcaggtgca tttttagcac atggattaca atcaagccgc ttgattgctt taaacaataa
    15481 agaaattacc gtccaagcga tgtctaaaga aagtgctgca cgtgtgacaa aacgttcggt
    15541 tgctgaaatc gaagcagctg ctgaaaaagt tccagctatc gcttacgata tcaataatta
    15601 tacaaaacta ggagcgatct atcatttcct agaagatatc accgaccaag ctgcttcaga
    15661 acaaaatgtc agcaaagtca tcgaagcaat caatgaagcc attcaagatg tccgcaatac
    15721 aaatgacacg atgctgacaa atcgatacac aaacgaaaca gccaaaacat tcggacgtgt
    15781 cgaaacaaat aaagtccgtg ccaaaatgga cgaagaatgg gatgcataaa aaatgattca
    15841 agcacatgat attgtccttt ttgataaaca tgaaatagaa gggttcacct tgcctgaatg
    15901 ggtcgttcta actgaaaaga tgttcggaac agttcgtcgt gcgtcgacgg atcacgcaga
    15961 aatactaccg attggtttac gtggacctgc ccgcaatcag agatggtcaa cagaaatctc
    16021 aaaacatgcc gtcaaacaag tcattcatcc gtgggacatc atcgaacaag aatcgtatag
    16081 acaagagaaa atcatggatt atcctgttta tcagcaattt catgctgcta gaaacatatt
    16141 atcaggatgt aaatggggaa tcggcggcag cttaggattt gaattatcga ctgggattcc
    16201 ggcagtaaaa gaaaccagtg attttgattt gctgctgtac gcaaattcac cgattgaatt
    16261 gccaattcag gtaattcaat cgcatcctgc tttttttgag caattcgata cccaagtagt
    16321 gactcggcaa ggcggttttt cactaaaaga gtatttgaga gagccagaga aaaaaattct
    16381 attgaaaaca gtcgaaggac ctaaattaac caaagaactt tggtaataaa gaaaaagaga
    16441 gagaaagaga tgtttgaaaa caaaaaaatc aaaatcaacg taagtgactt ccagaaagta
    16501 tcagctgtaa cggctgttat gatgcaaacg atattgagct ttgctttggc aaacagccac
    16561 agcgcaacaa atacagcttt tcttggtacc ttttacgtca tcgttaaata cacagcacca
    16621 atgtttattt ttgcaattgt ttataatatg gtaaaaacaa gtcagcattt gtcttattgg
    16681 gaatttttga aagaaaagtt ttttgaacta gtcgttccgt atcttctatg gacgacggct
    16741 tatctttggc tatttccagc agtccaacaa caaactccgt atacggatac tgcaagcttt
    16801 ttactgaaat atgtcacagg tgatggagcg cctcatttat ggtataccgt tatgatgctg
    16861 cagatccaat tgttcatgcc gttttttgtt tggctcggtt acaaagtatt tgctaataaa
    16921 aaatatgtat ggccagtatt gattggttca acgattttat atgtcgcatg gtatctgttc
    16981 tatcaaacac aagtattgac tgggccttat catgacagct ggtacttatt agatcggttt
    17041 gttgctagtt tcatgattta tggggtagcc gcatttgttt atcacgaaaa agtctatcaa
    17101 tatttggaca gagtacgtta tcttttctta cctgttgcct tagttatcgc gttcttttct
    17161 gtccgttcat tgctcgcaca tccaggagac ttgagctttg cgaatgcacc ttacttgaat
    17221 acgatccaaa gtttatacag tttagtcatc atctttgctg tcttcatagg cgcttccaaa
    17281 atgattgtaa acgattcgcc aaaattgcca ttgttcaaat ggttgtctgt ttatgcttat
    17341 cgtacgtatt tagccaatgt atttgttttc caagtactgt tattattgtt taaagatagt
    17401 tggctgcagt taccttcagg agtcatgatt cttgtagctt atctaatgac ggcaagttgt
    17461 gcatttgctt taagctggtt acttcatatc atttgggtag ctatcaaaaa aggtttcagc
    17521 aagtaatagt aggaagaagg agtgagtgtc atgataaatg acgaacgaaa aaaagcagtg
    17581 actcagcaac tcgctgcgtt ggtagaggaa gcattgatcg cagaagtaac attaagtcca
    17641 aaacctggat tagtggatgc actggatgcg ggtgcacatt ccgatatgga ctatgcttta
    17701 tttcttaagt ctgctaagac gctaactcct ttttttgaag aaatggctca aatagcatgg
    17761 catcatgcta ttgatcagga gttaagagaa agaattgctg agattgggcg aaaagcggaa
    17821 aaggcaatgc tgattgctac aaatggcatc aatacccata aaggagcgat ttgggcaatg
    17881 gggctattga tcagtgtcat tgcacaaaaa atcagtatgg aacagccttt ttctttatcg
    17941 aaaatacttg atgatgtcag caagcttgct gcttttcctg atacaaaata tcgtcataaa
    18001 aaaaccactc atggtcatag ggtgaagcaa aaatatggcg taaatggtgc atatgaagaa
    18061 gcgatattag ggtatccgca tatcaggctt gcaattcaag cagctaagga gctagataat
    18121 gattcatatg tacagaaaca aatgcatatg ttgttagttt tgatgagttc gctagatgac
    18181 acatgcgtat tgcatcgcag tgatcagaaa acgttagtgg aaatgcaaaa acttgctcat
    18241 gctgctagtc aaagtgcttt accaaatcgt ccatttacag cactgttgca cttttgtcag
    18301 gaaaaccaaa tctctcctgg aggaagcgca gatctcttgt ctgcaagctt gttcatgctg
    18361 aagatagagg aattatggac tgccgacaca tacatttgtc cgatgttact caatgactaa
    18421 ataagacaaa attgctaaaa aaacagttta tgcatctggt tattgactct tccttttagc
    18481 aaggctatca taaaaatagt tttttgtaat aaaagataac gaggaagaca atgaaaaaaa
    18541 tcaattattt agcaatcatt ttgttagtgg gcctatgttt gtttgctctg ccgacccaag
    18601 gattcgctgt cgcgggtggt catggtggag gtacatctag cggtcgaagt tcgggcagag
    18661 gttcatttgg tggtcgttca tatgggggca gttattttgg cagtcgttct tacggcagag
    18721 attcttatgg gtattatggt ccaggtacag gtccttctag ttatgagcaa acgggatttc
    18781 ttgcattagc tgttttgtca gtaggagggg tttctgcttt ttctatcgtc aagaataaaa
    18841 gacaaaagaa aaggctgaaa gcggagatat tgtcccgcat gtcaggaacg gctaaagaaa
    18901 agaaagcaca gtttctggaa atccaaaaga tattcatgaa gatccaatcc gcttgggaag
    18961 aagaaactcc tgaaaaagcc agaagctgtt atacagacaa gctgtttgcc gaacatcaga
    19021 aagttctgca aaaaaacaaa gcagacggtg taagaaatca tactaaaaaa ataactattc
    19081 aggaagtagg aaattatcga cagatccacg aaaatagttt tgccatccga attgatttca
    19141 gatgctgtga ttattcagtc gatcgaaaag atgggcgatt gatctcagga agtaagacac
    19201 aacgacagta tttttctcag atatggtatt ttgatttttc tgagaaagaa aaaagatggc
    19261 aagccgattt tattcagccg attatgctgg attgacagga aatatttatg aacggagttg
    19321 tgacaaaagc cttgtcgcag ctcctttttc tgaataaacg gtgtgccaga gatagcctat
    19381 cggcaataag gcacgatttt gtaaaatatg ggaagcgatt tttcaaaatc gcttcttatc
    19441 gctcaaggct gaacatctct gtcacaacct ctccactaaa tggtgtttaa aagcttgcat
    19501 ctgcgataat aagaacaacc aaacgaaaaa taggaagcac tattttagtt tagttgttct
    19561 tattacttga tgcaggacag ctttttcaca acctcttttt tgtatagatg ggcaaaattt
    19621 ttgtaataat aggcgtatgt ctttttcgtt gaggagatga aaaagcatat ggcttatttt
    19681 ttgtttgctt tgcttacttt ctttccactt caagtggcag aaatatctac tttttattat
    19741 gtaagcggaa ccatatactt gaaatcagaa ggagatcatt tctatgaaaa caagaacaat
    19801 tgaaatactt cacagaatca tgacagccca atatccagta aaagcaaaac atctagcaag
    19861 tgagttcaat gtcagtcagc ggacgatccg tcaggaagtt cttgaagcga atatctggtt
    19921 gagcagtaaa aagcttccgg agatccgtac aattcgcaac aaaggttttt tgttgaagct
    19981 ggaaaaggaa aagcaagctc gattagaaac tgaaataaaa gatgtgaaag acggcttttt
    20041 gaatcggcgc gaacgtattt ttgatctagt tttagccgtt gcttacagcc gaatcccgat
    20101 ccatctaaac aaaaaagaag aagaatttca aatctctaaa agtacattag atgaagatat
    20161 gagacggtta cgagcaacac tggtcagtta tgggatagag atcgtcagtt atggaaagca
    20221 aggactggtt tataaaggtt cagaacgatc tatccgtacg atgatctatg attttatcaa
    20281 tcagaatctg ggaatgaccg acttctttat gaatcaagat gcacaattaa caccggctca
    20341 aaaaatcttt ctccattatt ttcctttggt ggaaatcaaa aaaatacagg agatctattc
    20401 caataagttg ttgaaacaag aagatgatat ttataaaaaa caaatcctgc tttttacatt
    20461 gatctggatc cagcgaatca ggaaacatga atttatctct gccattaatt ggaaaaatac
    20521 agaaataaaa gacagtagct tttctgcatt tattgaccac atgatcgagg aatttcaatt
    20581 ggacaatgtc ccgcaagtag aaagaaatta tcttcgtttc actattgaga cgttcaatgc
    20641 tagagatatc tccaattcaa tcgagtgggt acaagctcaa ttactaacga tccaattgat
    20701 ccaatttgta gagaacgaga cacacgtacc tttccatcta aaggaagaag ctttattcga
    20761 aaatctctat aagcacatgg ctgcactgat tgtaagaatc aagaatcata tccaagtcat
    20821 gaatccattg aaagaaaaca tccgaacaaa ttatttccca atctaccgcg cagtcactca
    20881 ttttgttcct acgatcgaag aagtaattgg tggcaagata ctggaggacg agatagcttt
    20941 tcttgtcatc cacttttcga cgatcgccag tgtaatcaag cgagacttga attatgttta
    21001 taaatcagtc gttgtctgta accatgggat ggctactgga aatctgttag cagagaatct
    21061 gaaagaaaag tttcctcaga tcgagattac tgcagtttta agttcaaaag aaatcgaatt
    21121 agtagataaa ttggatgtca atctggtttt cagcaccttt caattgaatt atcgaaataa
    21181 gccgctttta gttgtcgatc cgattttaac tgagacgaat atcttggtta tagaagactt
    21241 tttgaaaaga aatgaagaac tacagcggtt agagcctaac agtcatgaat cgacggagtt
    21301 attcactcaa ataatagatg tcatgaaaga aagtggtggc cgagtagatc gaatcatcta
    21361 tggcaagtta gaaaaattat tcgaaacaaa caatttagaa atcaacaaaa gggagatcca
    21421 gcctatgtta aaagatattt tgacggacag caatatcatg atcaaagaaa aagccactac
    21481 ttgggaggaa tcgattgaac tagctgctag accattggtt aaagaaaaca ttgtcgaaga
    21541 gcgctatgtc gaagcgatga ttcgtgcagt gaatgattac ggaccgtata tcgtgattgg
    21601 caaacacatg gcactagccc atgcccgacc agaagatggg gtcaataaat taggagtcag
    21661 cgtagctacg attgagcaac caatcgattt cggtaatcct gaaatggatc cagtaaagat
    21721 tattttttgt ttggcagcag tagactccta ctcgcatctg aacatcatga aagaactaat
    21781 tgaattgatc aacaacgaag agaagttgga tcgattgatt gaatgcacaa atatcgaaga
    21841 tttcaaacaa ctgctttttt cagtagttga gaaataataa atgatacggg ggagaacaaa
    21901 gatgaacaaa ttgaacattt tattcgtatg cggtgcaggt ttaggaagta gttttgcagc
    21961 acagatgtct gctgaggatg ttttgaacaa attaggagta gaagccaaac tggaccattg
    22021 tgatatttct tccgcagttt ccatgaaacc agatgtgatc atcacagcag aaaattttcg
    22081 ttcgcagttc gaaaagttcg cagttgatcc taaaacggcg atgatctatt tgaaaaatat
    22141 tgtttcaaaa aatgaaattg aagaaaaaat caccccagtt ctgcaagata agggagttat
    22201 ctaaatacaa aaagaaaaga gggtaaaaa
//
LOCUS       DAYYBH010000045        22003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000045 DAYYBH010000000
VERSION     DAYYBH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.046"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1432)
                     /locus_tag="ACHAUG_001843"
     CDS             complement(41..1432)
                     /locus_tag="ACHAUG_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8480933.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1689..5078
                     /locus_tag="ACHAUG_001844"
     CDS             1689..5078
                     /locus_tag="ACHAUG_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8480934.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            5081..6502
                     /locus_tag="ACHAUG_001845"
     CDS             5081..6502
                     /locus_tag="ACHAUG_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8480935.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6499..8376
                     /locus_tag="ACHAUG_001846"
     CDS             6499..8376
                     /locus_tag="ACHAUG_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8480936.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8468..9310
                     /locus_tag="ACHAUG_001847"
     CDS             8468..9310
                     /locus_tag="ACHAUG_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8480937.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9462..10337)
                     /locus_tag="ACHAUG_001848"
     CDS             complement(9462..10337)
                     /locus_tag="ACHAUG_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8480938.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10544..11574
                     /locus_tag="ACHAUG_001849"
                     /pseudo
     CDS             10544..11574
                     /locus_tag="ACHAUG_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11663..11968
                     /locus_tag="ACHAUG_001850"
     CDS             11663..11968
                     /locus_tag="ACHAUG_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8480939.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            12147..14162
                     /locus_tag="ACHAUG_001851"
     CDS             12147..14162
                     /locus_tag="ACHAUG_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8480940.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            14164..14448
                     /locus_tag="ACHAUG_001852"
     CDS             14164..14448
                     /locus_tag="ACHAUG_001852"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8480941.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14474..15847
                     /locus_tag="ACHAUG_001853"
     CDS             14474..15847
                     /locus_tag="ACHAUG_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8480942.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15930..17945)
                     /locus_tag="ACHAUG_001854"
     CDS             complement(15930..17945)
                     /locus_tag="ACHAUG_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8480943.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17967..19181)
                     /locus_tag="ACHAUG_001855"
     CDS             complement(17967..19181)
                     /locus_tag="ACHAUG_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8480944.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19401..20345
                     /locus_tag="ACHAUG_001856"
     CDS             19401..20345
                     /locus_tag="ACHAUG_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8480945.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20641..21585
                     /locus_tag="ACHAUG_001857"
     CDS             20641..21585
                     /locus_tag="ACHAUG_001857"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8480946.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7490 a         4011 c         4144 g         6358 t
ORIGIN      
        1 ttttatcagg gtggtttata catagctgtt ttttgattat tcataactcg aataagcgga
       61 tgagttcttc ttcatttgcg aatgacacac tatcatcaat caatatatga ggaacatggg
      121 cattcaaaat gggttgattc gtaacgatca gatcatactc gagaagattg tatttcgaca
      181 gctgatcttc agtaatagca gttgtctcag agtgaatttt atgcacagca agaatatcac
      241 tgatatattc ttcaatatat tgactatgtc gaagacttaa gtcgctgata atcagtattt
      301 ttttatcaga agtataatgg tgaatttcag gattaccgat aagcatccaa aagatatagc
      361 tagataaata ataaatcaga ttctgattca gtagagtgga ataaaattga agttctgcac
      421 gaaatccagc atatagacgt atctggcggt gttgcatctg ctgtgcaaag taagttgcac
      481 ggatcgtcaa atgagtcgta tcgtatggga aaatacggga gaaaaaataa atttgcgtta
      541 ataattgaac aacatattct tcgttttttt ctttgcaatc agcggatgaa aacgaacagt
      601 aggacatatt ttttcgtatc tgctcactga tttcattttt ttctttctcc gaaaattctg
      661 aaaaaatcgt attgacaaaa tagttcacta cttttatata gctgctgtac ttcaattttc
      721 ctaagagatt tttcaagatt aagaagtctt ttttatcgat atttatcaac tgtacataag
      781 agtctccagc tttccattca aatggagttc cttgggattc tctcatgaga tgcacaacaa
      841 aatacataat aaagaaagat tgctcatatg ggctttcttc ctgaaacaaa ttcatctgac
      901 aattattctt gaccataatt tctttgatct tctttttttc gattggaaat ggcaacgtgc
      961 gtcttccgta gtaacttaaa aagaaaaagg taacaaagat acgcaactgg agctcacttt
     1021 taaggctagt taactgaaaa ccttggtcaa cgatcacttt taataaatag ctttctaaaa
     1081 tttgattgca ttgtttcaac ttacgtgtaa aagaagaccg gctgatggaa attttttcac
     1141 aataatactc aatcgtttta cccggattga caataatctc atttaataaa cgactgtttg
     1201 tagaactttg cgacatttcc atcagaacat ttaaataagc tggtacattc aaatgttcaa
     1261 gacgataccc caatttttgt gaagattgaa tcgttacatc cgttccccag cggattttga
     1321 tctcttcgat ataccttaca aatgtagatt ctgaactacc aattcgtttg gcaagttctt
     1381 ttttagtcat ccacttgtca gaaatggaca gaattcgaat gattttttcc atctttctat
     1441 agtttaattc caacaacctg atcatatgtt tttctccctc aacccttttc tttcaatgaa
     1501 acgctcatta atcgaaggaa tcaacttcat aaatgaaacc aattggtatg aaaattggtg
     1561 tgattccctt tagcgccctt cgtataataa ttatcatatt aaaataaata aaattatgta
     1621 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
     1681 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
     1741 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
     1801 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
     1861 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
     1921 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
     1981 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
     2041 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
     2101 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
     2161 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
     2221 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
     2281 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
     2341 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
     2401 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
     2461 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
     2521 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
     2581 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     2641 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     2701 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     2761 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     2821 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     2881 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     2941 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     3001 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     3061 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     3121 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     3181 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     3241 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     3301 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     3361 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     3421 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     3481 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     3541 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     3601 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     3661 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     3721 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     3781 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     3841 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     3901 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     3961 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     4021 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     4081 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     4141 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     4201 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     4261 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     4321 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     4381 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     4441 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     4501 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     4561 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     4621 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     4681 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     4741 gacaagaaac agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     4801 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     4861 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     4921 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     4981 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     5041 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     5101 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     5161 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     5221 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     5281 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     5341 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     5401 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     5461 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     5521 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     5581 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     5641 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     5701 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     5761 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     5821 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     5881 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     5941 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     6001 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     6061 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     6121 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     6181 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     6241 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     6301 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     6361 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     6421 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     6481 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     6541 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     6601 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     6661 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     6721 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     6781 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     6841 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     6901 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     6961 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     7021 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     7081 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     7141 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     7201 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     7261 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     7321 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     7381 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     7441 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     7501 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     7561 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     7621 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     7681 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     7741 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     7801 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     7861 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     7921 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     7981 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     8041 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     8101 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     8161 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     8221 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     8281 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     8341 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     8401 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     8461 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     8521 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     8581 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     8641 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     8701 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     8761 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     8821 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     8881 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     8941 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     9001 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     9061 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     9121 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     9181 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     9241 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     9301 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     9361 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     9421 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     9481 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     9541 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     9601 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     9661 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     9721 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     9781 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     9841 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     9901 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     9961 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
    10021 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
    10081 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
    10141 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
    10201 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
    10261 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
    10321 ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa cacttggttc
    10381 cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca aaccgaatga
    10441 taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact ttctgcgctt
    10501 ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta caatcgctta
    10561 tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa aacttacgaa
    10621 caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa cattcgaaga
    10681 catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc aattagttgt
    10741 catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg aacatggtaa
    10801 gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag agttatttgc
    10861 attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt tcgattctga
    10921 cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt tagaagtaga
    10981 aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat tttctactgc
    11041 gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt cttatttcgg
    11101 cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac gaatgaatga
    11161 ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat ccagttattt
    11221 ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aagggatgtt cgtgaaacaa
    11281 acaaaagatc gacaagaaga acatctgaaa atgttttaca tgcctgatca tgaagacttt
    11341 ggtatcgatt tgcccgaaca ttatggtgta ttgacctata tagatgaaca agggagttat
    11401 catgaagaaa aagtaatttc cgaggttggt gattacagcc gtgtctacga agggatttac
    11461 gagacattag ttaatggcgc agaaaaagtc atcaaagatg aagaaacaat cttacaaatg
    11521 aaaattttgg aagaaggaat caaaagcatt caagaatcag aaggaaaaat atgacatatt
    11581 tgtaagaatc gggtttgttt tttgacgcat aaactgtctt attgtacgat gaaaaagtat
    11641 agaactacta ggaggaacca acatgacaaa ttatgacggt gaaaaacaca aacgattaag
    11701 aggttcaatg gcccctaaaa cagaagaaga agtggcttta gcaggaacta attctggtga
    11761 aacagaagac aaagaagaat cattagctgg cacaaactct ggtgaaacag aaaataaaga
    11821 aacagcttta tctggttcaa attctgtaga agaggaaaca aagcctaatg agcccaatca
    11881 ctttgaaaaa gatatcgaaa aagcggttga agatgtcaaa gaaaaatttg aagaactgac
    11941 ctcaaaaaat aaacatgatc atcattgatc ggacgaaatc aattcataaa agcaggagac
    12001 atttggtcac ctgctttttt tctgtatcca ttcgatagaa atcgttcggt acagtcgtaa
    12061 aaaaaatcca actgtaagtt gtaccaaaca cccctcgatt taaaagcgct accatactag
    12121 aatataagta ggaggtgaac agcgctgtga actacaaaat tagttatctc ctgaaaacac
    12181 caagagaaat cagtctagaa aaagcagcag agatcatcga agtaaataca agcgaggcga
    12241 tcaaacaatt aactcctttt gccgaatatg tggatgatac ccgtatccgt ataaagaaat
    12301 tccgagcaat cgactgggca gaaaaatttt tggatattac tcgatttgaa gctgtgtata
    12361 ccgaacaaga gcgaagagat cttctttatt tgatggttta ttcgactgca acgtctgttt
    12421 ctgtttttca ttttcaagat tttcttcagg tttctaaagg gacagtcctg tcagatatca
    12481 aaaagataag aaatgaatta gaagtggaag attgtcagct gtcgtatact aggaaaactg
    12541 gttttaggct gctagggtca gaagcaatat taagaagaga agcaaaaaat cgcactgctc
    12601 gtttgatgca gtcacttgct ggaagatttg ggctcacgta tttaaccgca accctttcca
    12661 ttgattatta cgccaaagtt agagatttgc tagataaaac gatttcacta tcagatcttc
    12721 gattcgcacc aaatcgaatg gatgaattag cttattattt gagtttgaca accattcgaa
    12781 ttggaaaaac atccatggaa ctggtaaaag atgctgaact gttggcttct cttgcagtca
    12841 ctgacattag tcgacaagta ttgactggcc ttggctattc cgaacatcaa aattcggaga
    12901 ttatattttt ctctctttgc ttagcatcga tcgttgaagg agatgtccag gagcctgcac
    12961 ttgatttttt gctggaatgt tcttcagaag tcatccatcg catggaattc ctgatggccg
    13021 tagaatttgc ttcgtttcgt gaattattga tgaatgtctt ttatcacttg gtccctgcct
    13081 attttcgtat ttcctattct ttttatttac ctaatgtgat gatcgatcaa attaagcatc
    13141 aatatgcttc catctacgaa atgacaagaa aagcattact tccgttagaa agacgaatag
    13201 ggaagagcat ccctgaagaa gagattggtt ttttcactat actatttggc ggagagatca
    13261 gaaaagcgga cgcagaagaa agaaatagaa aaataagagc cgtgatcgtc tgtccaagtg
    13321 gcattagctc ttcattgatc ttaaaaagtg aacttcagca attattcccg atgatctttt
    13381 ttacagagac taattcagct gaccgcttac acgaggttga cgaaacttct tacgatatta
    13441 ttttttctac tgtacctctc acacttactg ataagaaaaa gtctctctat gttctgcatc
    13501 caatcatgac tgacttggaa aaaaatcagc tgatcaatca agtacaaaga gattggctga
    13561 taccaggatt ttcaatgcct tctgcccaag aattgttgtc ggcgctactg ccttatatcg
    13621 aattaaaaga aggcgtagac gaagaaaagc tctatcaagt attaaatcgt aaaatgaaca
    13681 aacttttaga aaagaaggag gatcaacgtc ctatgctatc tgaattatta acagaagaaa
    13741 tgatccaatt atcggatcaa ccaaaaaact ggcaagaagc aatcacattt gccgcacagc
    13801 cgctattatc agaaaataaa atagaagaaa gttatatcaa agctatgatc gagcgcgtgg
    13861 agcagtatgg tgcgtttatc catattggcg atcacatcgc attacctcat gctcgtccag
    13921 aagatggcgt aaacgaagta gggatgtctt tgctgaaact tcaacaacct gtggatctgc
    13981 ttgataatcc agcccatccc attagtttgt tcatctgttt agctgctatc gataatgagg
    14041 cacatcttcg ggctttggca aatttaacaa agctactttc taataaacaa aatcttcaag
    14101 atttattaca cgcatcaaca aaagaagaaa tattagcgat cataagaaaa ggagacgaat
    14161 gatatgaaaa tttttgcagt atgtcaaagt ggattaggaa caagttttat ggtagagatg
    14221 aacatcaaac aagcacttgc agatcttgga gcagatgtgg acgagtttga agtaagtcat
    14281 acggatgttg gaagtgtatc tagcgatatg gctgattatt tcttcattga gcattctttg
    14341 aaagatacgc taacttctat acctgatgaa aagctcgtac ctttgcaatc gatcattgac
    14401 agcgaagaag taaaagaaaa agtaacgaag atcttgtcaa aagaataaaa atgattcaag
    14461 ggaggaacta tcaatggata aattgttgaa tttgtttgtg tcaatcgtca gtcagccctc
    14521 catcctcgtt tctttgattg cactgcttgg tctcgttctt cagaagaaga aattttcaga
    14581 agtgatccaa ggaactatca aaacatttgt cggattccta gtactgatcg gcggtgctgg
    14641 tttaatcagt aattcactaa ccccttttgc ttcgatgttc caaacagctc tgcatactca
    14701 aggagtagtt cctagcaatg aagcagtagt ggcgattgct ttgcaacaat acggtacacc
    14761 tactgctttg atcatgctag tagggatgat cgtgaatatt ttacttgcta gattcacaag
    14821 atataaattc atttttctta caggtcaagc aatgctttat gtttcttgtc taacagcagt
    14881 cattttagta agcgcggggt ttactgcaaa tttgtggatg atcttattag gcggggtctt
    14941 cgaaggaaca ctattaacta ttacgcctgc tctttgtcaa ccgtttatga agaacatcac
    15001 tggaaacaat caagttgcgt tagggcatac cggaaatatc ggttatgctt ctagtggatg
    15061 gatcggaaag ttttttggaa acaaagaccg ttctacagag gacataaaga ttcctaaaag
    15121 tttcagcttt ttgcgagatt ctactgtatc gatcatgctc ttgatgtcga tcgtatatgt
    15181 cgcacttgct ttgttagctg gtcctggatt tgttgaaaga gaactaagca atggagacaa
    15241 cgctgtaatc tttgcactga tccaagctgg aacattcaca gctgggtttg ttatcgtcct
    15301 tcaaggggtt cgaatggtgt tggcagaaat cgttccagca ttccaaggga ttgctaaaaa
    15361 actcgtaccc gattctaaac cagcattaga tgttcctatc gtttttccat atgcacctaa
    15421 tgctgtatta attggctttt ttgttagctt ctttgtagga acactatcca tgtttgctat
    15481 gataggcttg caaactacag ttatcattcc tggggtagta ggacatttct tctgcggagc
    15541 tgcttctggt gtcttcggca actcgactgg cggtagaaga ggggcggtat taggttctgc
    15601 tttcaatagc ttattgatta gttggctgcc attatttatc cttccagtat taggagatct
    15661 taaaatcgct tcttctacat ttgccgatac tgattatcta gtaccaggac ttgtacttgg
    15721 gaaactagga gaatacggtc aaatagcctt gacagttgga atcatcgctt tctttttact
    15781 tgtcgtatta tctagctttc tttttgccgg aaaaagaaca aacattgttt ctaaagaaca
    15841 gaattaagca aacttcaaaa taaaaattgc ttggacaata tcagaagaag atttaaagtc
    15901 ttctgatatg tcgcaagcag ttttttgttt tatgcattta tttttgaata gcttgttaag
    15961 acaagtggct gttttgctag aagaggataa cttttaccat tcattttcac tgttccgcct
    16021 gttctatcag aagtaatcac gatcttatct tttgtttttt caagcgaaat ctttccatct
    16081 ttgataggta gcgtacaatt cagtgtttca aatgaatcta gattaggttg tatcataaat
    16141 tcttcataac ctggctttgt tggatacaaa ccgataaaat agcgtcctaa aagatagatt
    16201 gggcttgctc cccatgcatg gcataagctt tttccataag gatctccata catactgtac
    16261 ttttcatttc ctgtttgagt agggtcgtat tcttcccaga aagttaccgc atcatgagat
    16321 aacatccctc cccaataatc aagaatcgtt tggtagacga tattcgtctg tccgagtttg
    16381 cataaagcat cttgttcata gaatttgaaa tatggagtag taatctgtgt gatagtgtca
    16441 ttcaataaga cattctttaa gatcgattgc tgttggtctt tgttagcaaa atcgaataaa
    16501 acagcgaaaa tattcgcatg acgcgtcaca tttcttctcc cagattcata gcaatcgata
    16561 aaagcgccaa gttcttcatc ccaaaagtag tcaaataggt tttgtttcaa tagttgatat
    16621 ttttcttgat agagatgagt atgataattt tcgttctctc catatgtttc agctaagata
    16681 tctccgcaag tgatcatgga atgataggct ttcagtaata agacttgttc tgcagcaagc
    16741 gttccttctt tatccatttc agaccaatca ataaatatcc aatcttttgc tctcccataa
    16801 ataaacccta attcattcgt ttgctccatc aagtatctca ttaatgattg catcttaggg
    16861 tagacgatgc gtaaaaattc atagtctttc gtcatcatat agtggttttc gataccaatc
    16921 aaccacaaga tcgaatagtc aacgatagta ttcatatgct gcttgatttc attttgtcct
    16981 cttaatgcta atatcgttcg tttattgatt tcctcgtcaa aaaaaagata ttgattgatc
    17041 atgaatgatt gatacgcatc tccagaccaa atccagcgat ctcgtttgat accgtcgata
    17101 aaaaacaaat cactacataa cttgaaggct tcttctgata ctgaccagat ctgattcagt
    17161 aaagaagaat cgcttgaaaa agcagcttgt ttcgaaagag ataaaaattc atgaaaaact
    17221 tgaagttgaa ccattccagt agaaacctct ggtataaaca aatagcgaaa agcgcgttta
    17281 cgagctttca tttttcttgt cattcctatt tgcttatagt agcaataatc gatatccata
    17341 gcttctacat cggactctcc ataacataga gtgatagatt cgatatggtc atccagagat
    17401 attagttcta cacagccatt cactgctcgc ccaaaatcga ataagattcc tccgccaatc
    17461 tccgtttcag tgactggatg gacgcattct ttttgataat agatttgatt tggatctttt
    17521 gcgatatcga cataaagcgg actccagcca gctggatatt cttcaataaa attacttgct
    17581 gtccagccaa tatctgattt tacttcgtct ccctcgataa acatcgctgg tagccctgat
    17641 gtatgtccta caaagatacg aatttttgct ttaccagctg gacaagtcag ccattttcca
    17701 catggatgtt tttgtccatt aatttctaca tacccaactc cttggatcgt cactttaaac
    17761 atggaaggtt gatcaagaaa gtaatatctc ttaaacttga cgttccgatg gcaatcatcc
    17821 atgtaccagt acgctggcca gtcaaaacct cgttcttccc gttggaaatt ttgtagcagt
    17881 ccatggcgga tctcaaagtc tccaggatac caaagccatc tatctttatg tttttcattt
    17941 gtcattttac acgacctcca tcaaatttat tttgttgtca attgttcttt tatttcttca
    18001 tctactctta tcactgtctt tctattcgtc ctgtgtacta ctagaaggct aataagcgcg
    18061 ctaatcaaca gcaagatact gccattgacg aaaacagcaa gtgttttact cccaagagaa
    18121 gcaaaagaga gaaagagcaa accatatggt gcaatgaaag tacctaaatt agcgccaacc
    18181 aataagattg aagtattgaa actctccgcg tacttgggcg cttggatact tacttggttg
    18241 aataagtaag gaatgaataa cgaaaaagaa attcctgtta ggatcgcacc aaacccaatc
    18301 acatacaggt ttatcacagt ggcaatcaat acaaagccga ttcccatcag tagtaaagaa
    18361 agtgtaagca tatagcgatg tgcttttgtc tgaagccagc caaaacaaac tccggcagcc
    18421 atacttgcca attgcatgat tgttaagata ttcgctgcat tagtttcgtt tcctagagaa
    18481 taagtcgtca ggactgtggc taatttaatc gatacagcat tatataccac gatgaccagc
    18541 aagagaaaaa taccataact aatcatttta ggattcgttt tgattacttg tcttgttggg
    18601 gctgtgccat gcgattgaag ttcagttgtt tcaggtgtag gaacaaacaa aacaaacaag
    18661 acaagaatag gtaagctgat ggcgtagact aaaaaggtat attgccaacc gaactgtact
    18721 agctgtcctg caacaaatgt cattaaagct gcaccaaatc cttgaaatgc actttgaaaa
    18781 ccaatcatag aggctcgttc atctccgtga taaaaggcag caatcatgct gactgccaaa
    18841 gaattgatca atccaaagcc aatacctagt ccagctcttg aagctaagac aatactgtaa
    18901 ctttgtgaga agagggggag gataccacaa atcgatgcaa tcactaatcc gatgatcaca
    18961 gtttgttttt gtccgatttt cctagcaata aaactactaa tcaaaacaaa aatagtgacc
    19021 ataaaagagg gagcagtcgt taataattca acagcagata aagaacgcgt ggcataagta
    19081 gctgacatac tgggaattgc agacgtgact gcaccagcag aagtcaaaat aagagagact
    19141 gacagcagag aaaatttcat catcttttta ttcataagca tgttcctttc atttttgtaa
    19201 gcgcttatca aaaatatagt atgaagtggt tattctctca atagcggaat cacaggaagt
    19261 tcatgtccca accatagaaa gtctttatga aaccaaaaat agctttgcat ataatagcag
    19321 tacagcgtga actaataagc gaaagaaacc gcttacaaaa aggagggagt caattggaac
    19381 tagctgaatt agacattttt ttgcgtaacg aagaatcaat cgaaactctg cagcgtgaac
    19441 aaggaatgaa tatcaatgat ttaaacttag cttaccatct tcagacaccg ccaaaaatcc
    19501 accgagggga tctatttcaa cagggaaata ttcatatcag taaacaccgt cgatatgcgg
    19561 aagttcccga gcatacacat gattttgttg aaatcaatta tatgtatagc ggtacatgtt
    19621 tacaaattat taatggagaa aggatagaga tggaagaaag tgacgtggtg atcattgata
    19681 aagaagctac tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat
    19741 taaaagaaga aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac
    19801 tttcttcttt tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt
    19861 ttcgcggcgg aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt
    19921 ttgatacata cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg
    19981 aattgactca tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg
    20041 agatgcttag agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa
    20101 cagcagctca ttttggctac aatccggtct atcttagtaa taaattgaaa atgtattttg
    20161 atgcttcctt tcaagaactg gttctaaaga aaaaattcga ctttgcgttg gaactgattc
    20221 aagaaacgag tttttcgttg gaagaaatcg ctaatcgtat cggatataaa caaacgactt
    20281 ctttattcaa attattcaag aagtattcaa catgtactcc caatgaatgg agaaataaac
    20341 attaagaaga aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct
    20401 gtcttattat atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt
    20461 tgtattatca ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga
    20521 attcgtttcc atcaaaaatt ataataacaa ctattataat taacatgttg ataaaatgaa
    20581 taaacaagac tatacttttc atgaaagtaa caaatagtta tgaaaaggag ctgtttattc
    20641 atgaaaaaaa caagtcgtaa agtagtaatc gtcggtacag gttttgttgg aacaagtatc
    20701 gcttatgcaa tgatcaacca aggaatctct aatgaattag tattgatcga tgtgaatcaa
    20761 gaaaaagcag aaggggaagc attggacctt ttagatggta tggcttgggg cgatgaaaat
    20821 gtggctgtat ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt tgttatcaca
    20881 gctggtatca accaaaaacc tggtcaatct cgtttagatt tagttaaaac aaatgcgtca
    20941 atcatgcgcc aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat tatcgttgtt
    21001 gcttcaaacc cagtagatat tttgacatat atcgcttgga acgaatctgg tctgccaaca
    21061 tcacgtgtta tcggtactgg aacaacattg gatacaactc gtttccgtaa agaaatcgca
    21121 ttgaaattaa aagttgaccc acgcagcgtc cacggctata tcttaggtga acatggtgat
    21181 tcagaagttg ctgcatggtc acatacaact gtcggtggta aaccagtgtt tgaaattgta
    21241 gaaaaagacc atcgtattgc ccaagatgaa ttagatgtga ttgctgataa agtccgtaat
    21301 gctgcttatg agatcattga tcgtaaaaaa gcaacttact atggtattgg tatgagtact
    21361 gcacgtatcg taaaagctat cttgaataac gaacaagctg tattgcctgt atctgcttat
    21421 ttaactggtg aatacgacga aaaagatatc tttacaggtg tgccatcaat cgttgacgaa
    21481 aatggtgttc gtgaagttgt tgaattatca atcaacgaag aagaaaaagc aatgttcagc
    21541 aaatcaacaa gtgctttaag agaagtattg aacactgtat tataagaaac aatgaatatg
    21601 aactaaaaat cctaaaactg cttaacagct gaaatagctg ttaagcagtt ttttattgat
    21661 atctaatagg taagcaaata aatagaatgg ttaaaattaa tcggtccgaa aatgtatccg
    21721 tacatatgaa catatgaaat ttaatctgtt gattacaata agcgaatcgt tatcaatgtt
    21781 tatctgtggt taattaaaaa atccttccgc aacggcaaat acaatgattc atatctaatt
    21841 cgttcatatt tgattaactg aataaagaaa ctggttttta tcattcaatt aaacgacaaa
    21901 aaaagtaaga aaaactgcaa aattttttta taattttgca taagcagatt ttgatggaaa
    21961 aatgacgatt tttgataaaa aaccgttcga gagaggttta tca
//
LOCUS       DAYYBH010000046        21850 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000046 DAYYBH010000000
VERSION     DAYYBH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.047"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..1084
                     /locus_tag="ACHAUG_001858"
     CDS             80..1084
                     /locus_tag="ACHAUG_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480947.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1085..2029
                     /locus_tag="ACHAUG_001859"
     CDS             1085..2029
                     /locus_tag="ACHAUG_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480948.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2029..2991
                     /gene="opp4B"
                     /locus_tag="ACHAUG_001860"
     CDS             2029..2991
                     /gene="opp4B"
                     /locus_tag="ACHAUG_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8480949.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3018..3932
                     /locus_tag="ACHAUG_001861"
     CDS             3018..3932
                     /locus_tag="ACHAUG_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8480950.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3958..5739
                     /locus_tag="ACHAUG_001862"
     CDS             3958..5739
                     /locus_tag="ACHAUG_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8480951.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6087..6773
                     /gene="rnc"
                     /locus_tag="ACHAUG_001863"
     CDS             6087..6773
                     /gene="rnc"
                     /locus_tag="ACHAUG_001863"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8480952.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6793..10374
                     /gene="smc"
                     /locus_tag="ACHAUG_001864"
     CDS             6793..10374
                     /gene="smc"
                     /locus_tag="ACHAUG_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8480953.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10383..11192
                     /locus_tag="ACHAUG_001865"
     CDS             10383..11192
                     /locus_tag="ACHAUG_001865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8480954.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11203..12201
                     /gene="ftsY"
                     /locus_tag="ACHAUG_001866"
     CDS             11203..12201
                     /gene="ftsY"
                     /locus_tag="ACHAUG_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8480955.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12388..14655
                     /gene="gshAB"
                     /locus_tag="ACHAUG_001867"
     CDS             12388..14655
                     /gene="gshAB"
                     /locus_tag="ACHAUG_001867"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8480956.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14744..15196)
                     /locus_tag="ACHAUG_001868"
     CDS             complement(14744..15196)
                     /locus_tag="ACHAUG_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8480957.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15346..16296
                     /locus_tag="ACHAUG_001869"
     CDS             15346..16296
                     /locus_tag="ACHAUG_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8480958.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16289..17248
                     /locus_tag="ACHAUG_001870"
     CDS             16289..17248
                     /locus_tag="ACHAUG_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8480959.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17245..18000
                     /locus_tag="ACHAUG_001871"
     CDS             17245..18000
                     /locus_tag="ACHAUG_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8480960.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18023..18976
                     /locus_tag="ACHAUG_001872"
     CDS             18023..18976
                     /locus_tag="ACHAUG_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8480961.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19620..19916)
                     /locus_tag="ACHAUG_001873"
     CDS             complement(19620..19916)
                     /locus_tag="ACHAUG_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8480962.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20271..20627
                     /locus_tag="ACHAUG_001874"
     CDS             20271..20627
                     /locus_tag="ACHAUG_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8480963.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20637..21536
                     /locus_tag="ACHAUG_001875"
     CDS             20637..21536
                     /locus_tag="ACHAUG_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8480964.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         7224 a         3856 c         4658 g         6112 t
ORIGIN      
        1 ttataaaacg atttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga
       61 agcagagagg ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac
      121 aggattccga ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga
      181 caaaaacgag attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac
      241 tacgattatg ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa
      301 cgatctgaat ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat
      361 cggaatgatt ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca
      421 gatcaaagaa ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc
      481 actagaattg ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc
      541 ccatgaacta tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa
      601 acctccaatc gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca
      661 gattcttgat ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac
      721 tcatgactta ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca
      781 gttcgttgaa gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg
      841 ctctttactg aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga
      901 aggtgtcgtt ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat
      961 tccatggata ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa
     1021 ccacttggta cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt
     1081 gtagatgaca gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg
     1141 attcttcaat cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga
     1201 aaaaggaaag acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa
     1261 agccgttgtg ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt
     1321 aacgaagcga agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca
     1381 agattcaatg tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat
     1441 ccgaaatttt gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga
     1501 tatcgttggg atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca
     1561 acgtcaacgt ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga
     1621 tgaacctgta tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg
     1681 aatccagcaa gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa
     1741 acacatgtgc gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag
     1801 agaagacatt tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc
     1861 gcaaatcgat gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga
     1921 gtatatccaa aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac
     1981 gatcacaact acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg
     2041 attctgcgac gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc
     2101 ttattagccc aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat
     2161 ccggcagtga tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag
     2221 tacttccgtt ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa
     2281 cgctctgttg ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg
     2341 acagttgtcc ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa
     2401 aattcaatct tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta
     2461 tttatcttcg gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca
     2521 agtggatcac aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg
     2581 aaatatattc tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa
     2641 tatctgcgaa gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc
     2701 aaaggggtac cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg
     2761 atggcttctc aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa
     2821 aagatttttg gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac
     2881 tataccgtga tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta
     2941 ctttcagata tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca
     3001 gaaaggagaa tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc
     3061 ctatggggat acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat
     3121 taggtttgct agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag
     3181 acaaagtgat gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac
     3241 aaggacaaaa gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga
     3301 tcataggtgc tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca
     3361 tcggggttgg attagggatc atctctggct actacggagg gatcattgac aacatcttga
     3421 tgcggatcgt tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat
     3481 cgattatttc cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg
     3541 tagctaaagc ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca
     3601 gtgcctcaaa aacaatggga accaatgatt ttaaaatcat gttccgcgaa atcatgccga
     3661 atctaagttc attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg
     3721 agacaacttt gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat
     3781 tgatcggtta tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg
     3841 catcaatcct tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc
     3901 gttctgcaga tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg
     3961 aagaaaacga tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg
     4021 ggcggaggta cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa
     4081 ttaacttttc caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa
     4141 gctgcggtcg caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat
     4201 tcatatgatg cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat
     4261 ttcagaatca cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg
     4321 acaatcaaag taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat
     4381 gtgattttcc cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac
     4441 actttccgta acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc
     4501 ggaatcaaaa aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc
     4561 atgcttcaat cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt
     4621 gtagcaatca aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc
     4681 ccttattaca tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac
     4741 tactggaatg gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt
     4801 gcaactgaag cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact
     4861 tacgatacct ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat
     4921 acttatctag gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat
     4981 ccagattcaa aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat
     5041 aatgcagtag gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct
     5101 ccagtattcc agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa
     5161 gcgaaaaaac tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa
     5221 gacaaagatg gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg
     5281 caaccattgg cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac
     5341 acaacaggtc gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct
     5401 gaaattgatg tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta
     5461 tggggaccaa atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg
     5521 atcaacgata tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa
     5581 aaatggcaac aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa
     5641 gtattgccag tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca
     5701 tgggcaaatg tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa
     5761 gggcgatgtc ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc
     5821 aaggcgctga tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt
     5881 aagtcaaatt tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg
     5941 aaactaaacg aatttttcac aacttctcgt caatcactag gattattaaa gctttactta
     6001 ccttttgacc gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa
     6061 aaatatccat aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa
     6121 actataacat cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct
     6181 atgtgaatga gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag
     6241 atgccgtttt agaactgatg gtttctcagt atttattccg tttatatcct catttgccag
     6301 aaggaaaatt gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg
     6361 ccaaagaatg tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag
     6421 gacgtacgcg accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc
     6481 ttgaccaggg attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca
     6541 aagcaggtgc tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa
     6601 aatcaggtga tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac
     6661 gagtattttg gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat
     6721 cgaaaaaatt agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag
     6781 tgagggaaag ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac
     6841 aagacagtca ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga
     6901 aaaagcaata ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg
     6961 cgaggcggaa aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat
     7021 attgcagaag tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat
     7081 gagatcagtg taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa
     7141 caaagctgca gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa
     7201 tcgttctcaa tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac
     7261 cgtcgtggga tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa
     7321 gcagaacaga agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac
     7381 gaattggaag aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta
     7441 cggctaaaag aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca
     7501 gcaaaaaaag attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc
     7561 aaactatctg agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat
     7621 gcgcaagccg atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa
     7681 ctgaagcaga cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa
     7741 agctcgcaag aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa
     7801 aagcttcaag aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct
     7861 gaagcaaatc tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa
     7921 ttattggcag aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga
     7981 aacgaactga agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg
     8041 ttagccaagc aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa
     8101 ttgacacaaa agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa
     8161 atgatccaac aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg
     8221 tatcaattaa tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc
     8281 caagaaaact attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag
     8341 ctttcgggta ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta
     8401 gctatcgaag cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat
     8461 gctcggcaag ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca
     8521 ctgacgacaa tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta
     8581 gaagggttta tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg
     8641 gttcaaaacc tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc
     8701 gcgcaaacga tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc
     8761 ggaggatcca tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa
     8821 aatcaagaat tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca
     8881 caagaaaaaa aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa
     8941 gttttgcgta cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta
     9001 caaaacatca taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag
     9061 acacgtgaac tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac
     9121 aaacaaacag atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt
     9181 caagaaagcg accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa
     9241 caagcagatc ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt
     9301 gcccgagtcc aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg
     9361 acattgacag cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt
     9421 aacgaattat ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag
     9481 cgagatagaa ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt
     9541 gagcaaaaag cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa
     9601 atgattttag ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa
     9661 gcttcccaag actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc
     9721 ttaaaagaac agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa
     9781 caagtcagtg aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa
     9841 aaccaattgt ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa
     9901 gtatttgaag ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt
     9961 aaagctgaat tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa
    10021 gtacaacctc ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca
    10081 cttacagcca tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc
    10141 ttagatgagg tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg
    10201 agcgatttcc aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc
    10261 gcagcagatg tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct
    10321 gtacggatgg aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga
    10381 aaatgatcaa attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga
    10441 tttcagatga gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc
    10501 tatgtacagg acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag
    10561 atgaaggtga ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg
    10621 aaatcatgga aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc
    10681 agcaattgga tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg
    10741 ccccaaggaa gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg
    10801 atataagcga tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag
    10861 aagaattaga tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca
    10921 tcaaaacaag aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta
    10981 tttctttatt ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg
    11041 aggaaaatga tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg
    11101 tgcctttcgt caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag
    11161 ccaaagtgat ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa
    11221 atcaagcgtg cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac
    11281 gacaagggtt tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc
    11341 aattttcgaa cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca
    11401 gatgtaggtt ttgaaacagc gatacgaatc accgaagcat tacgtcaaga agtgaaactc
    11461 agaaatgcga aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc
    11521 tatgaagcgg aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca
    11581 gttattttgt ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc
    11641 catcagttca aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct
    11701 ggagcaatcg accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc
    11761 aatgcaggag gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag
    11821 caggcagatg ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg
    11881 aacgaattag aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa
    11941 acacttcttg tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc
    12001 aaagaaacaa cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc
    12061 ggtatcgtac ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt
    12121 gaaggaatcg atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa
    12181 ggattactaa aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag
    12241 ctccttgttt gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac
    12301 ttatttgtaa gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt
    12361 agattcatat gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc
    12421 aatgcgcgcc cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt
    12481 gatcttgcag gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat
    12541 cacccctata tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc
    12601 gattctattc ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct
    12661 ataccgaaag aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac
    12721 gaagagatca ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta
    12781 gcaaaagaat acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt
    12841 ggcgatgagt tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa
    12901 ttcaaaacag aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc
    12961 acttatttat tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat
    13021 ccgcaagaac ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat
    13081 gtgaaagttt cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa
    13141 gaagggaaac tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag
    13201 aaagtcgcag atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg
    13261 aatccttatg caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg
    13321 atgtacatgt tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg
    13381 actcgaaata acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa
    13441 ggtagagaaa ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct
    13501 ctttatttag tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg
    13561 aaactctatc aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat
    13621 caatattata aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa
    13681 ctttctactc agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta
    13741 gatgagtcgg atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat
    13801 gcaaacatga caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta
    13861 acgaaaaaag tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc
    13921 atggaagaag cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct
    13981 aaatcaacca attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat
    14041 tatcaagcag gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt
    14101 atgccgggaa cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg
    14161 cgagtccctg ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa
    14221 aaaaatagtg accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta
    14281 ggtgaattag aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa
    14341 gccaatcaaa ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc
    14401 gatatgactg atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca
    14461 ctaggagcta agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca
    14521 actgataaaa aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat
    14581 gtttatcctt ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat
    14641 cctgaagtat tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat
    14701 caaaagcttt ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc
    14761 agaagctttt tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt
    14821 attttttttc gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc
    14881 cgaattcctc aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt
    14941 tcgttgctag ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta
    15001 tggaagcatc tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga
    15061 tcatttcagc aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat
    15121 gctgttcttc agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta
    15181 catcatcttg tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag
    15241 catattccca ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac
    15301 ttaaaataag aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga
    15361 ctagtagcat tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta
    15421 caattgtcag aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt
    15481 ttgccacgaa cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc
    15541 atgcaacatt tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt
    15601 gcgcgtttag gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga
    15661 tccttgacag cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga
    15721 ttgttcctcc agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc
    15781 attggttcag ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca
    15841 tggcttcaag ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt
    15901 gttccattgt tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc
    15961 gaatcgtttg cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc
    16021 ttagtatcat tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga
    16081 gtgatcattc cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc
    16141 gtaactgctt tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg
    16201 atttttcctt atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc
    16261 ctttatcttc tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg
    16321 tatgcttgtt cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa
    16381 ctggcatttt gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct
    16441 agctgtcagc agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc
    16501 ttcagtattg ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat
    16561 cggaggagtg acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct
    16621 gatggtcggg ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct
    16681 ttttttatta ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt
    16741 tttacaagtc atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag
    16801 ctttggcaat atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg
    16861 gtacctgtta tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc
    16921 agtaaacctc gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct
    16981 tttgacaggg acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc
    17041 aaatatcagt tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt
    17101 tctggtagct atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg
    17161 gaatacaaca gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt
    17221 gattcgcgaa aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat
    17281 ggaaaatcag tggttaaaga tgtacagtta acgattcctg aaaagaagtt gactgctttt
    17341 atcggtccaa atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca
    17401 aaagatacag gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa
    17461 ttagccaaaa aattatcgat cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg
    17521 aaagaattag tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac
    17581 gaggaaatcg ttcaggaatc actggaacac attgggttag aagaaatagc ggatcaatcg
    17641 attgacactc tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa
    17701 gacacggatt atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt
    17761 caaatcatgc agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt
    17821 cttcatgata tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga
    17881 cagttattta cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta
    17941 tacgaaatgg acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa
    18001 aactaacatt aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc
    18061 atttggttta gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag
    18121 cagcacacaa actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt
    18181 gccaaataat ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc
    18241 tcttggtgtt ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc
    18301 atcctataaa aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa
    18361 tcagcttcag ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct
    18421 aagtcaaatt gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt
    18481 ccagcaaaat gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca
    18541 attagaggaa ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa
    18601 aaaagcacta gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag
    18661 attcggtttc atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc
    18721 cactcacggt caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt
    18781 tgtagtagac agaacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa
    18841 tgaactggta gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga
    18901 tgtctggtat ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa
    18961 tcaagcattt aaatagcata aataacagag gattaaactc tcatactatg taacaataat
    19021 gtttcttaca ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg
    19081 taaaatcgta ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac
    19141 gtgaattcat tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt
    19201 gtgatccatt ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg
    19261 aaacttagat aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg
    19321 atgttccatc tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat
    19381 tttctgtcca gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc tcttctaaac
    19441 gatgttccat ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta
    19501 ttttctgtcc agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa
    19561 caatgttcaa tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt
    19621 taggcatgtg tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg
    19681 gcgggaagct tactccaccg ttttagtaat tgatagaaat aactctcttc tgctttgatt
    19741 ttatactcgc ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa
    19801 atatccaatg ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa
    19861 ccctcgatat tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg
    19921 attcctccaa agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc
    19981 cgtgaacaaa aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta
    20041 ttttttgtta tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt
    20101 tttccgttca agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa
    20161 tcataataaa cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt
    20221 agttgcatct gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag
    20281 aagtgacaaa agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt
    20341 tattagatat cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg
    20401 atacggcatt tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt
    20461 ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca
    20521 tggatgatcc atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac
    20581 caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg
    20641 gaaaattggc gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc
    20701 tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag
    20761 atacggctaa taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat
    20821 tatctacgac atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga
    20881 tggaccattt tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga
    20941 ccgttctttt agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg
    21001 aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa
    21061 cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg
    21121 aagcaaaaaa acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca
    21181 tgtatcaatc tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat
    21241 cattcggaga attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc
    21301 atcgtcgctt tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt
    21361 attattatga atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct
    21421 atgatcctat gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc
    21481 aaacacaaca tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca
    21541 gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag
    21601 atccatttat cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg
    21661 atcggatgat ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga
    21721 taatcacaaa aaaaaatata atatacattt tttatttaat tataattaat attgattgaa
    21781 gtctaggcag tcaagtcgag agtgtaaaat attttgtgta aatgaaaaaa tccatacaaa
    21841 aaaggaagtc
//
LOCUS       DAYYBH010000047        21007 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000047 DAYYBH010000000
VERSION     DAYYBH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.048"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHAUG_001876"
     CDS             591..1280
                     /locus_tag="ACHAUG_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8480965.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHAUG_001877"
     CDS             1267..2475
                     /locus_tag="ACHAUG_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8480966.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHAUG_001878"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHAUG_001878"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8480967.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHAUG_001879"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHAUG_001879"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8480968.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHAUG_001880"
     CDS             complement(6578..7273)
                     /locus_tag="ACHAUG_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8480969.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHAUG_001881"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHAUG_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8480970.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHAUG_001882"
     CDS             complement(8472..8663)
                     /locus_tag="ACHAUG_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8480971.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHAUG_001883"
     CDS             complement(8684..9574)
                     /locus_tag="ACHAUG_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8480972.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHAUG_001884"
     CDS             complement(9561..10328)
                     /locus_tag="ACHAUG_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8480973.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHAUG_001885"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHAUG_001885"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8480974.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHAUG_001886"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHAUG_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8480975.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHAUG_001887"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHAUG_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8480976.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHAUG_001888"
     CDS             14899..15282
                     /locus_tag="ACHAUG_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8480977.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHAUG_001889"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHAUG_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8480978.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHAUG_001890"
     CDS             complement(16226..17044)
                     /locus_tag="ACHAUG_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8480979.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHAUG_001891"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHAUG_001891"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8480980.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHAUG_001892"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHAUG_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8480981.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18173..19507
                     /gene="dnaA"
                     /locus_tag="ACHAUG_001893"
     CDS             18173..19507
                     /gene="dnaA"
                     /locus_tag="ACHAUG_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8480982.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19705..20835
                     /gene="dnaN"
                     /locus_tag="ACHAUG_001894"
     CDS             19705..20835
                     /gene="dnaN"
                     /locus_tag="ACHAUG_001894"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8480983.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         6006 a         4264 c         3846 g         6891 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgcggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gagtcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa tggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggtgatttc ctgtgtattc cgtctcctaa ctattggtat atctatattt
    17941 tttatccaca gatgcaagag gaaatctatt gatttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    18061 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    18121 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    18181 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18241 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18301 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18361 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18421 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18481 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18541 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18601 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18661 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18721 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18781 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18841 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18901 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgcctcgttt ctcgctttgc
    18961 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    19021 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    19081 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    19141 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    19201 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19261 ttatcacatc caattgaaag atatcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19321 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19381 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19441 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19501 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19561 aaaagttatg cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19621 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19681 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19741 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19801 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19861 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19921 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19981 tttactttag aagttttaga aaataaacaa gtagcgatta cttctggaaa agcgaatttt
    20041 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    20101 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    20161 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    20221 ttattggctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20281 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20341 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20401 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatcctga taccaatcgt
    20461 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20521 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20581 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20641 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20701 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20761 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20821 gtacgtacaa attaacaatg attttcattg ttcttgaaaa tttaattgaa aagaaataga
    20881 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20941 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    21001 ttttatt
//
LOCUS       DAYYBH010000048        20360 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000048 DAYYBH010000000
VERSION     DAYYBH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.049"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACHAUG_001895"
     CDS             291..1430
                     /locus_tag="ACHAUG_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8480984.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACHAUG_001896"
     CDS             complement(1497..2072)
                     /locus_tag="ACHAUG_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8480985.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACHAUG_001897"
     CDS             complement(2077..3051)
                     /locus_tag="ACHAUG_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM8480986.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACHAUG_001898"
     CDS             3334..4302
                     /locus_tag="ACHAUG_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8480987.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACHAUG_001899"
     CDS             4415..4693
                     /locus_tag="ACHAUG_001899"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8480988.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACHAUG_001900"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACHAUG_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8480989.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACHAUG_001901"
     CDS             5150..5905
                     /locus_tag="ACHAUG_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8480990.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACHAUG_001902"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACHAUG_001902"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8480991.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACHAUG_001903"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACHAUG_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8480992.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACHAUG_001904"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACHAUG_001904"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8480993.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACHAUG_001905"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACHAUG_001905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8480994.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACHAUG_001906"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACHAUG_001906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8480995.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACHAUG_001907"
     CDS             10642..11184
                     /locus_tag="ACHAUG_001907"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8480996.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRMQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACHAUG_001908"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACHAUG_001908"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8480997.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACHAUG_001909"
     CDS             12777..13679
                     /locus_tag="ACHAUG_001909"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8480998.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACHAUG_001910"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACHAUG_001910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8480999.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACHAUG_001911"
     CDS             15122..15544
                     /locus_tag="ACHAUG_001911"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8481000.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACHAUG_001912"
     CDS             16013..16243
                     /locus_tag="ACHAUG_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8481001.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACHAUG_001913"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACHAUG_001913"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8481002.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACHAUG_001914"
     CDS             17654..17830
                     /locus_tag="ACHAUG_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8481003.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACHAUG_001915"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACHAUG_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8481004.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACHAUG_001916"
     CDS             18325..18708
                     /locus_tag="ACHAUG_001916"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8481005.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACHAUG_001917"
     CDS             18877..19761
                     /locus_tag="ACHAUG_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8481006.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACHAUG_001918"
     CDS             19875..20111
                     /locus_tag="ACHAUG_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8481007.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACHAUG_001919"
     tRNA            20199..20279
                     /locus_tag="ACHAUG_001919"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACHAUG_001920"
     tRNA            20286..20357
                     /locus_tag="ACHAUG_001920"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3565 c         4425 g         5611 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcatgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DAYYBH010000049        19694 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000049 DAYYBH010000000
VERSION     DAYYBH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.050"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACHAUG_001921"
     CDS             complement(<1..210)
                     /locus_tag="ACHAUG_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8481008.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="ACHAUG_001922"
     CDS             complement(216..446)
                     /locus_tag="ACHAUG_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481009.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="ACHAUG_001923"
     CDS             1063..1725
                     /locus_tag="ACHAUG_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481010.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="ACHAUG_001924"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="ACHAUG_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8481011.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="ACHAUG_001925"
     CDS             5145..5459
                     /locus_tag="ACHAUG_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8481012.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="ACHAUG_001926"
     CDS             5472..5846
                     /locus_tag="ACHAUG_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8481013.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="ACHAUG_001927"
     CDS             5856..6200
                     /locus_tag="ACHAUG_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8481014.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="ACHAUG_001928"
     CDS             6271..7623
                     /locus_tag="ACHAUG_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8481015.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="ACHAUG_001929"
     CDS             7683..8825
                     /locus_tag="ACHAUG_001929"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM8481016.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="ACHAUG_001930"
     CDS             8850..9104
                     /locus_tag="ACHAUG_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481017.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /gene="mobT"
                     /locus_tag="ACHAUG_001931"
     CDS             9360..10544
                     /gene="mobT"
                     /locus_tag="ACHAUG_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8481018.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="ACHAUG_001932"
     CDS             10541..10678
                     /locus_tag="ACHAUG_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8481019.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="ACHAUG_001933"
     CDS             10683..10904
                     /locus_tag="ACHAUG_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481020.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="ACHAUG_001934"
     CDS             10917..11417
                     /locus_tag="ACHAUG_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8481021.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="ACHAUG_001935"
     CDS             11514..13361
                     /locus_tag="ACHAUG_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8481022.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="ACHAUG_001936"
     CDS             13364..14260
                     /locus_tag="ACHAUG_001936"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8481023.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="ACHAUG_001937"
     CDS             14310..14699
                     /locus_tag="ACHAUG_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8481024.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16185
                     /locus_tag="ACHAUG_001938"
                     /pseudo
     CDS             14686..16185
                     /locus_tag="ACHAUG_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="ACHAUG_001939"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="ACHAUG_001939"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8481025.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18872..19694
                     /locus_tag="ACHAUG_001940"
                     /pseudo
     CDS             18872..19694
                     /locus_tag="ACHAUG_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DAYYBH010000050        19078 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000050 DAYYBH010000000
VERSION     DAYYBH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.051"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACHAUG_001941"
     CDS             6..245
                     /locus_tag="ACHAUG_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481026.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACHAUG_001942"
     CDS             312..521
                     /locus_tag="ACHAUG_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481027.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACHAUG_001943"
     CDS             581..1252
                     /locus_tag="ACHAUG_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8481028.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACHAUG_001944"
     CDS             1305..3281
                     /locus_tag="ACHAUG_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8481029.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACHAUG_001945"
     CDS             3294..4049
                     /locus_tag="ACHAUG_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8481030.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACHAUG_001946"
     CDS             4046..4870
                     /locus_tag="ACHAUG_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8481031.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACHAUG_001947"
     CDS             4880..5128
                     /locus_tag="ACHAUG_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481032.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACHAUG_001948"
     CDS             5171..7270
                     /locus_tag="ACHAUG_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFM8481033.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACHAUG_001949"
     CDS             complement(7308..9365)
                     /locus_tag="ACHAUG_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFM8481034.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACHAUG_001950"
     CDS             complement(9479..9691)
                     /locus_tag="ACHAUG_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481035.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACHAUG_001951"
     CDS             9878..10345
                     /locus_tag="ACHAUG_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481036.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACHAUG_001952"
     CDS             10342..10584
                     /locus_tag="ACHAUG_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFM8481037.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACHAUG_001953"
     CDS             10602..10904
                     /locus_tag="ACHAUG_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481038.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACHAUG_001954"
     CDS             10976..13030
                     /locus_tag="ACHAUG_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481039.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACHAUG_001955"
     CDS             13030..15642
                     /locus_tag="ACHAUG_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8481040.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACHAUG_001956"
     CDS             15639..18170
                     /locus_tag="ACHAUG_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8481041.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="ACHAUG_001957"
     CDS             18222..18554
                     /locus_tag="ACHAUG_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481042.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..>19078
                     /locus_tag="ACHAUG_001958"
     CDS             18555..>19078
                     /locus_tag="ACHAUG_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481043.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILG"
BASE COUNT         6854 a         2966 c         3803 g         5455 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgatccta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    13141 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    13201 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    13261 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    13321 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    13381 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagg
//
LOCUS       DAYYBH010000051        18567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000051 DAYYBH010000000
VERSION     DAYYBH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.052"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHAUG_001959"
     CDS             6..305
                     /locus_tag="ACHAUG_001959"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8481044.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHAUG_001960"
     CDS             378..1739
                     /locus_tag="ACHAUG_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8481045.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHAUG_001961"
     CDS             1761..2021
                     /locus_tag="ACHAUG_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481046.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHAUG_001962"
     CDS             2035..2787
                     /locus_tag="ACHAUG_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8481047.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHAUG_001963"
     CDS             complement(2881..3486)
                     /locus_tag="ACHAUG_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8481048.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHAUG_001964"
     CDS             complement(3539..4513)
                     /locus_tag="ACHAUG_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8481049.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHAUG_001965"
     CDS             complement(4891..5610)
                     /locus_tag="ACHAUG_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8481050.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAUG_001966"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAUG_001966"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8481051.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHAUG_001967"
     CDS             7161..8558
                     /locus_tag="ACHAUG_001967"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8481052.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHAUG_001968"
     CDS             8639..9079
                     /locus_tag="ACHAUG_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8481053.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHAUG_001969"
     CDS             9227..9553
                     /locus_tag="ACHAUG_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8481054.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHAUG_001970"
     CDS             9554..10303
                     /locus_tag="ACHAUG_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8481055.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHAUG_001971"
     CDS             complement(10383..10838)
                     /locus_tag="ACHAUG_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8481056.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHAUG_001972"
     CDS             complement(11265..11540)
                     /locus_tag="ACHAUG_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481057.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACHAUG_001973"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACHAUG_001973"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8481058.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHAUG_001974"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHAUG_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8481059.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACHAUG_001975"
     CDS             14770..17436
                     /locus_tag="ACHAUG_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8481060.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACHAUG_001976"
     CDS             17800..18552
                     /locus_tag="ACHAUG_001976"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8481061.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
BASE COUNT         5965 a         3212 c         3833 g         5557 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatg
//
LOCUS       DAYYBH010000052        18557 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000052 DAYYBH010000000
VERSION     DAYYBH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.053"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..799
                     /locus_tag="ACHAUG_001977"
     CDS             152..799
                     /locus_tag="ACHAUG_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8481062.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    928..1144
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1192..3837
                     /locus_tag="ACHAUG_001978"
     CDS             1192..3837
                     /locus_tag="ACHAUG_001978"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8481063.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4147..5460
                     /locus_tag="ACHAUG_001979"
     CDS             4147..5460
                     /locus_tag="ACHAUG_001979"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8481064.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5450..6103
                     /locus_tag="ACHAUG_001980"
     CDS             5450..6103
                     /locus_tag="ACHAUG_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8481065.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6158..6850
                     /gene="radC"
                     /locus_tag="ACHAUG_001981"
     CDS             6158..6850
                     /gene="radC"
                     /locus_tag="ACHAUG_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8481066.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            7000..7200
                     /locus_tag="ACHAUG_001982"
     CDS             7000..7200
                     /locus_tag="ACHAUG_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8481067.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7454..9133)
                     /locus_tag="ACHAUG_001983"
     CDS             complement(7454..9133)
                     /locus_tag="ACHAUG_001983"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8481068.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9135..9347)
                     /locus_tag="ACHAUG_001984"
     CDS             complement(9135..9347)
                     /locus_tag="ACHAUG_001984"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8481069.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9740..11470
                     /gene="efrA"
                     /locus_tag="ACHAUG_001985"
     CDS             9740..11470
                     /gene="efrA"
                     /locus_tag="ACHAUG_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8481070.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11467..13227
                     /gene="efrB"
                     /locus_tag="ACHAUG_001986"
     CDS             11467..13227
                     /gene="efrB"
                     /locus_tag="ACHAUG_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8481071.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13322..13519
                     /locus_tag="ACHAUG_001987"
     CDS             13322..13519
                     /locus_tag="ACHAUG_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481072.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13672..14157
                     /locus_tag="ACHAUG_001988"
     CDS             13672..14157
                     /locus_tag="ACHAUG_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8481073.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14261..14854)
                     /gene="clpP"
                     /locus_tag="ACHAUG_001989"
     CDS             complement(14261..14854)
                     /gene="clpP"
                     /locus_tag="ACHAUG_001989"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8481074.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15034..15561
                     /locus_tag="ACHAUG_001990"
     CDS             15034..15561
                     /locus_tag="ACHAUG_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481075.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15652..16389
                     /locus_tag="ACHAUG_001991"
     CDS             15652..16389
                     /locus_tag="ACHAUG_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8481076.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16393..17310
                     /gene="miaA"
                     /locus_tag="ACHAUG_001992"
     CDS             16393..17310
                     /gene="miaA"
                     /locus_tag="ACHAUG_001992"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8481077.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17312..18541
                     /gene="hflX"
                     /locus_tag="ACHAUG_001993"
     CDS             17312..18541
                     /gene="hflX"
                     /locus_tag="ACHAUG_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8481078.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5995 a         3209 c         4033 g         5320 t
ORIGIN      
        1 tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt tttatttgtc
       61 tcacaccctt ttccttccta cttgtaacct tgctcccgtg ttagaaacat gttacactaa
      121 atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata cactgccaaa
      181 agcaactgcg aaaagattac ctttatattt acgctacttg aagatgctgg atgactcagg
      241 tataagtaga atcaaatcaa atgagttcag cgaaatcacc caaattcctt ctgcaacgat
      301 ccgcagagac ttctcgcaat taggcgagct aggcagaagc ggatatggct atgatgtccc
      361 ttttcttatc gatgtattca ataatatcct taatactaaa gaagaaaaac gaatcgcatt
      421 agtaggatat ggaaatcttg gcaaagcatt gaaacacaac aattttagaa gaaatgaaaa
      481 tctaaatatc gtgtgcgtct ttgataatga tccagcactg atcaatcgag taattgatgg
      541 agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa atgtgacagt
      601 agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa ttgtcaaagg
      661 aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag cttatgtcaa
      721 tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg atgagaatta
      781 ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga gaactcgtcg
      841 taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac cacttgttga
      901 ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag taactggcac
      961 attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc agtgaaggta
     1021 gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg ttaactcact
     1081 tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat catacgtcct
     1141 agaatacgaa agtattttag gacgtttttt agtttaaagg aggaaaacag catgacggaa
     1201 gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg ttatcagcat
     1261 tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc ttattcaatc
     1321 gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg ggatacgacc
     1381 ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt atggctgcct
     1441 ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct tagagagcaa
     1501 gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg ggaatggaaa
     1561 gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc tttagattac
     1621 agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa agtttttgtt
     1681 accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg ggatcctcaa
     1741 gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg cgccttttat
     1801 catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac aacccgtcca
     1861 gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg ttatcaagcg
     1921 ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat cattgcagat
     1981 gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc acatgatcca
     2041 aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat ggatgatgat
     2101 ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc tgctcgtaaa
     2161 gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac gatgatccac
     2221 agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc tactcaatgg
     2281 tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac agatcaagca
     2341 gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga aaatgtccat
     2401 gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg gtatcacaaa
     2461 gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa ctgggtacaa
     2521 gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc aacaatgggc
     2581 tggccagata cggaaagcga agattataag cgatacttcc ctactagcac tttagtaacc
     2641 ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt ggaatttact
     2701 ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga acaagggcgg
     2761 aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga aaagtatgga
     2821 gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga tgtccgcttc
     2881 agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa tgcttcacgt
     2941 ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc tggagaaaaa
     3001 tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg agtaactgag
     3061 ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt tatctgggat
     3121 gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga tgatgctgtt
     3181 tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct acgtttattg
     3241 catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca tcagggaaat
     3301 tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga aacagcttct
     3361 aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg ttcagaagtg
     3421 aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc taaaatcgat
     3481 caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga agagttgacg
     3541 atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac aggtgcagaa
     3601 atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg attagaaaaa
     3661 gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc taatgaacgt
     3721 tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga aaaagattat
     3781 ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat tcaataagaa
     3841 tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat tgaacatcgt
     3901 tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg tgacataaat
     3961 tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg aggtggatca
     4021 tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt cttttctctt
     4081 tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga aatgggggaa
     4141 ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc atttggttca
     4201 cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc agagagaaaa
     4261 gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc ttatttgaga
     4321 agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta tatcgaaacg
     4381 ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt gatccagctt
     4441 gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt agctggtatt
     4501 actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa gaaagtggac
     4561 gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt agctgtacca
     4621 atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg agatacaatt
     4681 gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt agtaacagga
     4741 aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa aaatgcaccg
     4801 ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga gatgtgggga
     4861 gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac tccgctttta
     4921 gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt ttactgtcaa
     4981 ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa atctcgatgg
     5041 ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg tgcccataat
     5101 acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg atatcaaatc
     5161 aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga acgattgcag
     5221 caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc catcgaatta
     5281 agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg gcaattcgga
     5341 ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac cggttcactt
     5401 tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca tgaagtataa
     5461 tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt attacgaagc
     5521 cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt atttaaaatt
     5581 cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct cggaatttgg
     5641 caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg aacgtttttc
     5701 gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt tagaagaaca
     5761 tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac ttttattgaa
     5821 gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg ttcaacgagc
     5881 aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag cgaagaaaga
     5941 aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg ctgagatccc
     6001 ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa aaacagctgg
     6061 ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca tctcttttcg
     6121 tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat tgatcaaaga
     6181 agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg aagctttatc
     6241 ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata gtgtgatgtc
     6301 gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac aggctacttt
     6361 gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa tcaaagcctt
     6421 gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac cggttcgtac
     6481 cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac aagaacattt
     6541 ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag tgttcatcgg
     6601 ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg tacgttattg
     6661 tgcagcaagg atcgttttag cgcataatca tccttctggt aatgttattc catcgcagca
     6721 ggatatgaat tttaccaaaa ggatacaaaa gtgtggagaa atgatgggga tcactgtact
     6781 ggaccacttg atcatcggac gcaaacgata ttttagtttg agagaagaag gaatgatgga
     6841 agagaagtaa gttgctttct tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt
     6901 ttgtttggga atagaagttg aaacatctgt tgcacctcta tcgtacacga gcaatgttac
     6961 aatttaaatt agtgcttttg gcacgaggag gatattatta tggaaaacgg aacagtaaaa
     7021 tggttcaacg ctgaaaaagg atttggattt atctcacgtg aagatggaag cgacgtattc
     7081 gttcacttct cagctatcca aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg
     7141 actttcgacg ttgaagaatc agaccgcggc ccacaagctg caaacgttgt aaaagcttaa
     7201 ttttatctgt aaatcaaatt gatggtagac atcaattttt acgagagaac aaagcgagta
     7261 acaaaaatac ctgccacaag agacaatttc tcttgtggca ggtatttttt tgtatgaaaa
     7321 agtaaataaa tttaagaggc tatgaaagag ccaccttgta tcaaggaata agaacaaaca
     7381 aaaatggctt ctcatgtttt tcatttgttt tctcttatat tcaggacaag gtgctgattt
     7441 cataacctct tatttattct tcatctggtg tcattaccat tggcaaaatc attggatgtc
     7501 tttctgtacg ttcgaataag aaaggctgaa gcgcgccagt catcgcttct ttcaatttat
     7561 attctgaaca gtcaggtcca ttcaaacatt ctcttaaagc gtggaataag attcgctgag
     7621 cttcattgat catttctcct gattctcgca tgtagacaaa tccacgagag agaatatctg
     7681 gaccagccaa gatttctttt ttcttgatat ccactgtcgc gacagctaat acaagacctt
     7741 cttctgaaag caggtgacgg tcacgtaaaa cgacattacc aatatcaccg atcccgctac
     7801 catcgatata cacatctcca gccacaaaat gcccagcagg acgcgcagaa tcgctagtta
     7861 acgctaaaac atcgccattt tccatgatga agcagttttc tgcaggaaca cctacatctt
     7921 gtgctagaga agcatgtact ttcaacatgc ggaattctcc gtggacagga ataaaatatt
     7981 ttggcttcat caaacgaagc attaattttt gttcttcttg tccaccgtga ccggaagtat
     8041 ggatgttatt gatttttccg tgaatcactt ctgcaccagc ttcagacagc aaattgatca
     8101 aatgattgac acttgttgta ttaccaggaa taggtgaact tgaaaagatc actgtatcgt
     8161 ctggctggat ttggatttga cggtgagtac cattcgcgat gcggctcaac gcggccatcg
     8221 cttctccttg agaacctgta cataggatca tcgtttcatt ggcaggcaag cgattgattt
     8281 cgtgtgcatc tacaaatgtg ccgtctggaa ctttgatata tcctaaacgt tgaccgttga
     8341 cgatggcatt ttccatgctt cgtccgaaaa cagcgatttt acgaccagtt gccacagcgg
     8401 catctgctgc ttgctgcaaa cggaagatgt tagaagcaaa gctggcaaaa atgatacgtc
     8461 cttcgatttt tgaaaagatc ttcaaaatag aatgcccaat cgttttttct gatttagtaa
     8521 atgttggaat ctcagcattt gtactatcag ataaaaggca caagactcct tcttccccga
     8581 tacgagccat tttatgcaag tttgctggtt cgccaacagg agtgaaatcg aatttgaagt
     8641 ctccagtcgc tacgatattt ccagaaggtg ttttcaccac gatccctaat gcatctggga
     8701 tactatgggt cgttctgaaa aagctgacag aagtctttct aaaacggatg atcgtatctt
     8761 cattgatctc atggagctct gcatctcgaa gcaagccatg ctcatctaat ttgtttgtga
     8821 tcaacgctaa tgcgagagga ccagcataga taggaatatt tgcttgtttc agcaagtagg
     8881 gtattccgcc gatgtggtct tcgtgcccgt gagtaatcac caaggctttt actttgtgta
     8941 agttttgtat gatgtagctg tagtcgggaa tgacatagtc aattccaagt aaatcgtctt
     9001 ccgggaattt gatcccggca tcaatcaaaa tgatttcatc ttggaattgt accccatacg
     9061 tatttttccc gatttcacct aatccaccaa tagcaaagac gcctgtctcg ttatttttga
     9121 tggaaagttt catattaaaa ctccactaat gagaaatcag cattttcttt ttcgtactct
     9181 agatgacttc cttctagaag ttgaacgaat tcaatattgt atggggtatt ttcttccaca
     9241 cgttggcgag cgtctacatc gctttttgcc tcaatgtaaa gagattttgt ttcttcccgt
     9301 ttagggttgc gtacttttgt ttcttgataa taaactttat agatcattgt tttgtctcct
     9361 ttatctgcgt aaagcagtga tttagctagt tgattttttt agacaacaca aaacgagggg
     9421 acaagccacc tggtttttca tatgctttga ctagaatact ggattcgagt caaagagaca
     9481 agcctcgttt ttgcttatct gttcggatat tttttcaacc gtaccttctg tataaaaaat
     9541 atacaacaat taattgttat tctagcataa aacagcaaat aagtatagaa atcaactaga
     9601 ttgaaagaaa aaatcaatgt ttatcatccc gctataatca taatgacttt tttatgagag
     9661 aagtgttcta ttaataggct cgttgtgcta tcatttttag taatgaaaat gtaacaaaag
     9721 attaaaagga gatgagaaag tgaaactaat gtggcattat accatgcgtt ataagaaatt
     9781 cttgtttttc aatttcatat gcgtatttgg ttttattttg attgaattag gacttcctac
     9841 gattttagca cgtatgatcg atgtaggaat ccgcaatgat gattttgatt atgtgaagca
     9901 acaagggata tttatgatcg ttattactgt aatcggtatt attttgaata tcttgttagg
     9961 ttattttggt tcgaggatta cgactaatat cgtagcagat attcgagatg atttgttcga
    10021 aaagatccaa agttattcgc atcaggaata cgaaaatcta ggcgtatctt cattgatcac
    10081 tcgtacaaca aacgatgctt atcagatcat gttgttcctg caaaatattt tacgtatcgg
    10141 ttttatggca ccaatgatgt tcattgtcag tttgtacatg gtcatgcgta caagtgtgac
    10201 actcagtttg tatgtagtag gtgcattacc tttactatta ttggcagtcg tagcgattgc
    10261 taaggtgtct gaaccgttat ctaaaaagca acaaaaaaac ttagataaaa tcaacagtat
    10321 cttgcgggaa aatctttcag gattgcgtgt gattcgtgca ttcgtcaatg aaaagtttga
    10381 agaaaaacgt tttgctgtag tcaatgagaa ttatgcttct agttcaaaaa gcttattccg
    10441 tttgatggct gttgcacaac cgggttttta tttcttattt aatatcgtaa tggtattgat
    10501 catctggagc ggggcagtgc aaatcgatgc aggcgagctt cgtgtaggag atttgattgc
    10561 gtttatcgag tatatattcc atgccttgtt ctcattcatg ttatttgcta gtgtgttcat
    10621 gatgtatcca agagcagctg tttcagctgg acgtatccaa gaagccctag atgccaaacc
    10681 ttcgattgta gaagatccaa atggtatatc tgatacaaaa actaaagggt atatcgaatt
    10741 tcaaaatgta acatttgctt atccaggcca ttcgcaagag ccagtgatac gaaatgtcag
    10801 ttttacagcc tctccaggag agaccgttgc ttttattgga agtaccggta gcggaaaatc
    10861 tacgttgatc caattgatcc ctcgctttta tgatgtgagt gaaggaaaga tcttgatcga
    10921 tggagtagac gtgagagatt atcggctaag caagttgcga gataagattg ggtttattcc
    10981 gcaaaaagcg ctgctattta ctggaacgat tgcagataat ttgcgatacg ggaaagaaga
    11041 tgctacgcaa gaagaaatgg aacgtgcagc tgagattgct caagcttcag aattcatttc
    11101 tcgaaaacca gatggattta atgaacattt atcagaaggt ggttccaatt tttcaggtgg
    11161 acaaaaacaa cgattggcaa tagccagagc catcatacgc cgtccggaaa tctatatttt
    11221 tgatgacagc ttttctgcat tagattatca aacggatgcg aagttacgtg ctcgtctgaa
    11281 aaaagaaacg acagaatcta ctgtattgat cgtagcacaa cgtgtgggaa cgatcatgca
    11341 tgcagataag atcatcgtat taaatgaagg agaagtcgtc ggtatgggga cgcatcggga
    11401 gttgttggaa aaatgcccga tttattatga tattgccgct tctcaattat caaaggagga
    11461 attagcatga aaaaaacctt ttcttcctta aaacgtttag ctcgatatat cagaccgtat
    11521 cgtgagactt ttcttttagt gatcctattt acagttctga cagtggcttt caacgtggct
    11581 ttgccatatg ttacaggact tcctactaca gaaatcagcc gtaatattgc caatggcgaa
    11641 tcaatcaatt tttcttatat attcaaatgt ctcatttgga tcacagttgt tggaataggg
    11701 tattgtattt cgcaattact ttctggtgtt ttgatgacaa atgtcgttca aagtgccatg
    11761 catgatttga gaagagacat agaagaaaaa atcaatcgtc ttccggtttc atatttcgat
    11821 aaaaaccagc aaggaaatat tttgtctcgt gtgacgaatg atgtagatgc agtcagcggt
    11881 gctttacaac aagcttttat tggtatcgta aatgcgatat tgggaattac gttggctgca
    11941 gcaatgatgt tttatatcca gccgatgatg gcgctgatct ctatgatcat gattccaaca
    12001 tcgatctgga tctcaaaacg agtgatcaat gcttcgcaga aatattttca aagcatgcag
    12061 aattctcttg gtgagctaaa cggctacgtc caagaaaata tgacaggttt tagtgtattg
    12121 aaagtatatg gacgcgaaaa agaaacattg gaaggattca aaaaagttaa tcacagcttg
    12181 aaatactttg ggtttcgtgc agcattcatt tctggcttga tgatgcctct agttcaatta
    12241 actgcctatg ctacttatat tggtatggct gttctaggaa gcttttatgt tattactggg
    12301 gcgatcgttg tcggtcaact acaagcattc atccaatata tttggcaaat cagtcagcca
    12361 atggggaacg tgacacaatt gtcttcagtt ttgcaaagtg catcagcggc aacgaaacgt
    12421 gtattcgaga ttttagatga gccagaagaa aaagaaaaca aagtagatgc ttcactgcca
    12481 gaaacgattt taggagatgt gacattcgat catgtcgatt ttgcatatga tccgaagaaa
    12541 ccattaatcc aagatttgaa ttttgaagtg aaagctggtc aaaccgtagc tgtggtcggt
    12601 ccgaccggtg ccggcaagac gacattgatc aatctattga tgcgttttta tgacgtagac
    12661 aaaggagcaa taaggattga tggaatcgac accaaatcaa tgagtcgcag tgatgtgcgt
    12721 tcgctatttg gtatggtctt gcaagatgca tggctttatc aaggaacgat tgcagataat
    12781 atccgcttcg gaaagcttga tgcgacagat tatgaagtag ttgatgctgc gaaaacagca
    12841 aacgtagatc attttatccg aacgatgcca gatggatatg atatggccat caattctgag
    12901 ggagacaatg tgtctttagg acaaaaacag ttgctgacga ttgcccgagc agtggtctcc
    12961 gatcctaaaa tcttgatttt agatgaagca acaagttcag tagacacccg tttagaagcg
    13021 ttgattcaaa aagcaatgga tcgcgtgatg gagggacgga caagtttcgt catcgctcac
    13081 cgtttatcga caatccgtga tgcagatctg attctagtga tggatcaagg tcagatcatt
    13141 gaaaaaggaa cgcatgagag tttattggca caaggtggat tttatgaaaa actgtataac
    13201 agccaatttg ctgaggaagc agaataagtc atataagaat aaaacagttt aaaacttatt
    13261 ttttgttctc tattcttgat aatggtaaag taaagttatc aagaaaagtg aggacacgga
    13321 gatggaacag tttttaaatg aaaaaattac tgaagccaaa agagaagttg atcgtttaac
    13381 gcacgtctgt cagacgaagc cttttgattc agctgatttt tttgaaaatg agtttcaaat
    13441 cgaacgcttg gcagcacaaa tagaagtgta cgaattaatt ttaaaaaaag taatagaaga
    13501 aaatatagat gtacaataaa aacaggtaaa acgtcagtta ccagatactg taaatagaca
    13561 tctggcctga gcaggtttta cctgtttttt ttcttatgaa ataaatatat gctatgatac
    13621 ctttattcaa taggattagt ttctacaaaa aatgaaagga gaaaaataaa tatggaacgg
    13681 tttatagcat caaatgccac agtgattggt gatgtgacat tgtcagaaga tgtgacgatc
    13741 tggtatcaag ctgttttacg cggggacagt aattggataa aaataggaca aagaacgaat
    13801 atccaagatg gtacaatcat ccatgtcgat catgatgcac ctgtagatat tgcggagaat
    13861 gtgacggtag gacatcagtg catgctacat ggctgtacaa tcgaaaaagg ggcgctgatc
    13921 gggatgggga ccacgatatt gaatcatgcg gttattggtg agaatagttt gattggtgct
    13981 ggatcattag taacagaagg aaaagttatt cctcctaatg tcttggcttt tggtcgccca
    14041 gctaaagtga tccgtccttt gacaaaagaa gaaatacaaa aaaacaaaga aaacatccag
    14101 cattatgtcg agataggtcg gatgcatgcg caaggaaaat tcaaggagca aagataggca
    14161 gtagcatcca taaaataaaa aaggatggtc agtttgagtt gaccatcctt ttttatttgg
    14221 tttagataag agagcttaaa aaagcttcct ttgaaacaga ttaatttaat gaagaactgt
    14281 tttccatgac ttcatcgatc aatccatatg cttttgcttg ttcagctgtc atatagttgt
    14341 cacgatctgt atctttttca atcacttcaa gcggttgtcc agtgcgttca gctaaaattt
    14401 tgtttaagcg ttcgcgtgtt tgcaaaatat ggcgtgccgc aatttcgatt tcagttgctt
    14461 gcccttgagc gccgccaaga ggttgatgga tcatgatctc agcatttggt aaagcaaatc
    14521 gtttaccttt tgtaccagct gtcagcaaga agcttcccat tgaagcagcc atacccataa
    14581 caattgtttg gacatctgct ttgacaaagt tcattgtgtc gtaaatagcc atacctgctg
    14641 taactgaacc accaggagaa ttgatataaa gatagatatc tttttcagaa tcttgagcgt
    14701 ctaagaaaag caattgggcg atgatcgaat tagcaagatc atctgttact tggccgctta
    14761 acatgatgat acggtctttc aataaacgtg agtagatatc gtaagctctt tcacctcgtg
    14821 aagattgttc aatgactgta ggaattaaat tcataataga gttcctcctt gatttcgttt
    14881 agaaatagtg taccataatt tagtttctaa tacctaaaat gtagtataca tttttggtca
    14941 aaaaaggtca aataaaaaac ttaggcaaat tagtcgcata aacccgcttt atggtatgat
    15001 aagcagagaa acaaaacaag gaggaacaac agattgatag ttggtattac tggccttatc
    15061 ggagtgctga caggtgctgt agttgtctat atcggcatga tactgcaaat gcgtcacttg
    15121 gaaaataaag aacagcgtag acgtgaattg gaattgaaag tcaaagaaat cgagacgctg
    15181 aatcaattaa ataaaaagat caacgagatc ttgcaaaaac ggacaacgct gatggaagaa
    15241 tatgtcagct ttgatgcatt tgatgattgt tatatcacga ttgacgattt tgcgtatctc
    15301 caatcatttt cagcgcaaaa tagtttttat ctgccaaatt atattttaga cgagctattt
    15361 aagaatatct cccatcgaaa agtgatttta tctccagatg aaacaatgaa gattggtgga
    15421 tatgcttata aaggcggacg cgtgatttta gagaatttta cggatagtct gactgaaatg
    15481 atcaatgaaa aaaaagctca gttgaagact atgacgcgcc agccgattgg tttcttctcg
    15541 aaagatgatg tttggagctg atattttgaa gcattgccgt tgcacatgag agcgtggtaa
    15601 tgctttcttt ttttcttaat gatacaatag aactgaacgg agtgaagaaa gatgttacaa
    15661 atctatgccc atcgcggaag cagcggcacc cacccagaaa atacgcttcc tgcatttgca
    15721 gaagctgtac gagtaggtgc agatggtatc gaactagatg tccatctaag caaagatggt
    15781 tatttgatcg ttatgcacga cgaagaagtg gaccggacga ctaacggtaa agggctgatt
    15841 cgagaaaaaa cattggaaga actcaaaaaa ttgaatgcag ggggcaaatt cagcaaagaa
    15901 tttccagcag ctaaagttcc tgaattgaga gaagtgttag gactgctcct ccaaaaaaat
    15961 taccgaggga tgctaatgat tgaaattaaa actgatcagt atgaatatcc cggtatcgaa
    16021 gaaaaattag ccgaaacatt aaaaagtaga gattggcctt ttcaacatgc atattgcagc
    16081 tttcatttgg attctttgga aaaaatcgca ttactggaac cggagataca gctggactta
    16141 ttgatgagta cttctgataa aaagatccaa ctagcagcag aaacccctta tatagaagga
    16201 gtacatccaa aaaatacttg gttgttcgaa catccagtcc aggcagctga ttttccaaaa
    16261 gcaattcgtg catggacgat taacgatgaa gaagaaatgg ccaaatgtca agaactcaaa
    16321 atagatggaa taataactga ttttccggag aaagctcttc ggtggaaaca atcaacgaaa
    16381 agtaggtaaa atatggaaaa agtattagtg atcgtcggtc cgacagcagt cggtaaaaca
    16441 gcgctaagtg tggaactcgc acaactattt ggaggagaga tcatcagtgg tgattcattg
    16501 caaatctata aaaatctgga tatcggtaca gcaaaaatca aagaagaaga gatgaataat
    16561 gttccgcatc atttgattga tgtgatcgaa cctactcaac gttactcagc cgttgatttt
    16621 cagcattcag gtcgacaatt gattaccgag attgcgaacc gagggcacct acctatcata
    16681 gctggtggta cgggtctcta tatccaatcc cttctatatg attatcagtt aggagcgaca
    16741 gaagaagcga atcctgagat tagaaaaaag tatgaaacgt tagctgaaac aattggttcc
    16801 caagcattat ggaatcagtt gaaagaaaaa gacccggcag ctgctgagaa aatccactgg
    16861 aacaatcaac gcaaagtgat aagagctctg gaagttttag agacgacagg acatagtatc
    16921 acggcaccta aagaagagcc aaagcaattg tatgattacc tcatgattgg attagatacg
    16981 aagcgtagta ttctttatga acgaatcaat caaagagtcg atttgatgct agcagaaggt
    17041 ttagtagcag aagcgcgtaa ggtgtatgag cttggagaag tccaggccag tcaaggtatt
    17101 ggatataaag aatttttccc ttattttgaa gggaaagaaa cattggaaga agcaacagaa
    17161 caagtcaaac tacattcgag aagatacgcg aaacgtcagt tgacgtggtt tcgtaaccgt
    17221 ttggatgcac agtggtttga tcttataagt gagccaaatc aaaaaagtca gattgagcaa
    17281 acaatcaaaa aatggctgga ggcggaataa aatggaagaa aaagtgatca tcgtaggggt
    17341 agaaaccgaa gcaaaccagc gttacttttc agagtcaatg gaagagctag ttcagctgac
    17401 gaataccgca tcaggagaag tcgtattcac aattacacaa aagagaccac aagtggatcg
    17461 gcaaacgatt atcggtaaag ggaaattaca agaattagtt caacaagcgg atgcgcatga
    17521 agcggatttg atcatcttta atcatgagct aactccgaga cagagccaac tgattaccga
    17581 tgctgttggt cttccggtaa ttgatcgcgt gcagcttatc ttagatattt ttgcgatgcg
    17641 tgctcgttcg aaagaaggga agctgcaggt tgaacttgct cagttagaat acctgctgcc
    17701 tcgtttagta gggcaaggga aaacattatc tcgtcttggc ggtgggatcg gaacaagagg
    17761 gccaggtgaa acaaagttag aaacagaccg ccgacatata cggaataaaa tttttgctgt
    17821 taagaaagaa ttgaaagaag tagaagctca tcgagagcgc aatcgccaaa aaagaaaaaa
    17881 tagcgagatt tttcagattg gacttattgg ctacacgaat gccgggaagt caactatctt
    17941 gaatctattg acacaagcgg atacttattc gaaagatcaa ttgtttgcaa cgttagatcc
    18001 attgactaag agatggcgtt ttgctgaagg atttgagatt actgtaacgg ataccgtcgg
    18061 gttcatccaa gacctgccta cgcaactgat cgatgctttc cattctacat tagaagaaag
    18121 tcaaaatatg gatcttttgc tacatgtagt cgatgctagt tctccagatc gtattttaca
    18181 ggagcagact gtcttaaaat tgatggatga actgaatatg aaagaaatgc cgatactgac
    18241 tgtttacaat aaggcagacc agatcgaccc agcgatgttc acgcctacgc tattcccaaa
    18301 tgtattgatt tctactcaga cgaaagaagg taagatggcg ttgatcgaag caatcaaacg
    18361 tcagctgatg gaactaatga ccccttatac actttctgtg ccaagcaacg aaggacagaa
    18421 attaagcgag ttgagaagac aaacaatggt tttggacgaa gcgtacatgg aagaaacaaa
    18481 cgaatatgaa gttaaagggt ttgcaaaaac caattcaaaa tggttaagaa aaaaccaata
    18541 aaattttatt tttttaa
//
LOCUS       DAYYBH010000053        16558 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000053 DAYYBH010000000
VERSION     DAYYBH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.054"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACHAUG_001994"
     CDS             14..1141
                     /locus_tag="ACHAUG_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8481079.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACHAUG_001995"
     CDS             complement(1359..2501)
                     /locus_tag="ACHAUG_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8481080.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACHAUG_001996"
     CDS             complement(2506..2691)
                     /locus_tag="ACHAUG_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8481081.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="ACHAUG_001997"
     CDS             complement(3045..3290)
                     /locus_tag="ACHAUG_001997"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481082.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="ACHAUG_001998"
     CDS             complement(3284..3685)
                     /locus_tag="ACHAUG_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8481083.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACHAUG_001999"
     CDS             complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACHAUG_001999"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8481084.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5703..7469)
                     /locus_tag="ACHAUG_002000"
     CDS             complement(5703..7469)
                     /locus_tag="ACHAUG_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8481085.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7482..8276)
                     /locus_tag="ACHAUG_002001"
     CDS             complement(7482..8276)
                     /locus_tag="ACHAUG_002001"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8481086.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8286..9662)
                     /locus_tag="ACHAUG_002002"
     CDS             complement(8286..9662)
                     /locus_tag="ACHAUG_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8481087.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9782..10429)
                     /locus_tag="ACHAUG_002003"
     CDS             complement(9782..10429)
                     /locus_tag="ACHAUG_002003"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8481088.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="ACHAUG_002004"
     CDS             complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="ACHAUG_002004"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8481089.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="ACHAUG_002005"
     CDS             complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="ACHAUG_002005"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8481090.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13096..14727)
                     /locus_tag="ACHAUG_002006"
     CDS             complement(13096..14727)
                     /locus_tag="ACHAUG_002006"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8481091.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            15031..15702
                     /locus_tag="ACHAUG_002007"
     CDS             15031..15702
                     /locus_tag="ACHAUG_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8481092.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(15862..16053)
                     /locus_tag="ACHAUG_002008"
                     /pseudo
     CDS             complement(15862..16053)
                     /locus_tag="ACHAUG_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         5370 a         3200 c         2222 g         5766 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
     3781 cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag gctaggctaa
     3841 tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg
     3901 atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga
     3961 ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat
     4021 tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt
     4081 aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta
     4141 ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc
     4201 catctagctc ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc
     4261 aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg
     4321 gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca agttctttag
     4381 ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat
     4441 ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga
     4501 tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat
     4561 tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa
     4621 taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg
     4681 atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag
     4741 cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat
     4801 aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa
     4861 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
     4921 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
     4981 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
     5041 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
     5101 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
     5161 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
     5221 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
     5281 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
     5341 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
     5401 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
     5461 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
     5521 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
     5581 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
     5641 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
     5701 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
     5761 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
     5821 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     5881 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     5941 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     6001 tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta
     6061 attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact
     6121 tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca
     6181 gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta
     6241 tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg
     6301 tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct
     6361 tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac
     6421 cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag
     6481 ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat
     6541 ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac
     6601 atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca
     6661 gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta
     6721 aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg taatccagct
     6781 tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta
     6841 ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta
     6901 gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa
     6961 actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta
     7021 ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca
     7081 gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct
     7141 acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca
     7201 attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca
     7261 gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat attactcaag
     7321 taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa
     7381 gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa attatatggt
     7441 ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt
     7501 ttccaaaaag tatctatata tttttgttca atatctacat aatctcccga taaacattta
     7561 aataaaacat cagttcttaa gttttcccat gattcttttt ctttattgac caaaatagga
     7621 taaaagaaaa ctttattttt atttgcagct tctagatcac caggagaatc accaatcatt
     7681 aatatcttat ccaaatcaca accattttca attacacttc gaataatatc ttcttttttt
     7741 cctttatctt gacaacacag ttcatcaact aatgataata attcatgtct ttcccattct
     7801 tcttgaacag cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga
     7861 gcttttatag tatctctagc acttaaaaag ggtttatcta atccttttgc ttgactaatc
     7921 tgttgattga cttcttccga ccattttaaa gcttttaata aatcctctga ctgagaatat
     7981 tctatttccc tcttcaaaga tggattagac aattcttttg ttagagatac ccagtgcttt
     8041 aatcgattaa tcgatgttac atcatttttt tctaatgttt taactaaacc taaaaatctg
     8101 tttattccac gagtctctga atataaatta atattattcc aatcctgtag gaatttttct
     8161 ctatgatgaa catcaaaaat ctctgcagca attggtccga aaaagagatt gtgtttataa
     8221 gtcatcgtat ccataacaca gccatctgaa tcaatacaaa atatccaatt attcatattt
     8281 ctctcctatt tatgtttttg ttttaaaagc tcgcaatttt ctaagacttt atctttagat
     8341 aatggataaa ggaaaaataa aattaatcct acaatcagaa aacaaattga tggaattaat
     8401 gttgaagcgt tatataactt ttctaaaaca tctcccgttt gtacatttgc taaagagtca
     8461 tagcctataa tagaaagagt ccaacctccg acgcctccag ctagagcttg acctattttt
     8521 ctagcaaaag aatataccgc atatacggtc ccatcttctc tttgatcaga ctttacttct
     8581 tgatcatcaa taacatcagt aatatttgcc caaataacag tattaaacac acccaaaccc
     8641 atatagccaa ccgaactaag aattaagaat acatacatat tactagcttt tatcaaaaat
     8701 aagatcaaat atactattgc tgaaaagaac attcctactg atgccagttc tttttttcca
     8761 tattttttac ttaaaaacaa agttaaaggc gaaacaacta ttaaaaccaa aataggattg
     8821 ataaaattca ttaaaactat tccctgtgca tcattatagt aatcaggaaa tacataatta
     8881 ttcatactca taattaataa ttgagcaagg agtaagaaaa tagccgcacc aattatagaa
     8941 agtaatgccc tatttttaac taaccctttt aaagatttta acaatgaaac tttgtattga
     9001 gattggtcaa ttcttactcg ttcagtagtt agtttataac atattaaata acaaataagt
     9061 gcacatatcg aaaatattcc cgcaacaatt gtaaaagttg atccaccacg aactatttga
     9121 gcgcctgaat catctcttgt ataaataaat aatggtgtta atacaccaat gaccaaactt
     9181 gctaaagtag ctccaacatt tcggaatgtc gatagcattg ttctttcttc tgtaatagaa
     9241 gtaatagctg atgccattga tccataagga atatttatag atgtgtaaaa aatactcccc
     9301 cataatatat acgtaacata catgtatact accttaacgc tcatagaagc tgaaatcata
     9361 gaagtttgat acattaaaaa acttgctaaa gcgactggac cactcattct taaaatccac
     9421 ggtttaaact taccctgctt agttgctttc gatctatcaa caatacgccc catagttaca
     9481 tcagtaactg catcgataaa tcttgctact aaaaataatg ttccaacggc agctccgctt
     9541 atccccaata cttttgtata aaagatcatt aaaaaagaac tagcaaaaat gaaagtaaaa
     9601 tcgtttccaa aatctccaaa catatatcct attttgtctc ttataccaaa ttttctttcc
     9661 atacaattct ctcccttctg ttgttaagcg ttttcattct gatgaaagta cgtactatac
     9721 tctgaagcgt aaagagtcag tatttatttt ttacatttca gagtattatt tgttttttct
     9781 cctaataatt ctttatctca taaaacttat ctatatattt actagctact cgacttactt
     9841 cttcaaaatc acctttttca acagaagcca ataaattccc acctacacca actgcaacaa
     9901 ctcctacttc aaaccaatct tctaagttct ctatacttac tccgccagta ggcattatat
     9961 ttacgtgtgg taatggagct tgataggctt ttacaatgga tggaccaaaa gcagaacctg
    10021 ggaatagttt aatgatatct acgccagcag taagtgcctc ctttatctca gtaattgtca
    10081 tacaacctgg taaataagga atttgatata aattacaaat ctcagctgtt tgtcgatcaa
    10141 aagatggact aacgatatat tctgccccag ccataattgc gatacgagca gtaaacgcat
    10201 ctaaaaccgt accggctccg attattacat ctttttgatc agcatatttt ttagcaattt
    10261 ctttgattat ttcttcagca ttaggaactg taaaagttag ttcaatccct ttaattccac
    10321 ccgcaataat tgaatttatt gctttaatgg cctcatcctt atttttccca cgtacaaccg
    10381 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
    10441 tcctttatgg atacatactt tgtctagctg tactaactta caaagaataa atttaagctt
    10501 ttatttcaag tattattttc tacttccgat ttcaaaaaaa ttaaaataat tatacgcatt
    10561 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcggcc
    10621 tgctttaacc cactcgctta gtaattgaca taatactcga cgaaaatact catggcgagg
    10681 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
    10741 ttgtgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
    10801 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
    10861 tggaagccaa tcagttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
    10921 ttcagcggta tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
    10981 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
    11041 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
    11101 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
    11161 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
    11221 accgtgatct gctaatcgac agccaatttg atgaaaatga tccactcgtt ggctcaatgc
    11281 ttgcttcaac ccattaaagt ctttgattgg tatagccgct gtttgactaa gttttaaaat
    11341 atagttcgaa aagcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
    11401 taccttgaaa gaattatcct gtttagaaag ctcagtatga taccacaagt catctgtagg
    11461 atcatccgtt gtacagatgg ctttcacatc catcatctcc aacaattctt tcgggcgata
    11521 ggaatccgtt tgaatttttt cgttagcacg tcgccaaatt tctggtgcat tcttctcaga
    11581 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
    11641 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcatcacc
    11701 tggaccactg atcaactctt cttctatacc attagctcgc atcaatcgcc atttataatg
    11761 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
    11821 tagcaaatat gctgggtcta aatgacaatg atagtcgata attggttggt gttttgcaaa
    11881 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
    11941 atttaaacac tcctttcctt ccgttagttt ttagaagaga aaatttttcc tctaaataaa
    12001 ctcccttata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
    12061 aaactgcacc tagagctcgg tcatataatc cataaccagg tctaccggtt tctccccaaa
    12121 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
    12181 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttcg taaaaatcga
    12241 gtccattgtc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
    12301 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
    12361 tacataatgt aataccatta gaaggagaat ccactacatt acacaaccaa tctaaatcgt
    12421 cacgattttt aacaatacga ggtaatccga aaatggaata tggtggatca tctgggtgaa
    12481 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
    12541 agttctctcg taatttctct tcatcaactt ctttataggc ttctaataat tgcttaattt
    12601 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
    12661 ctctaataat ttcctccgga tcagtaggga tattttttga aatataggct aacgtagaag
    12721 acccatcatc taaacgataa tccaaatccg attttaccca atcaaatact ggcataaaat
    12781 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
    12841 caatgtattg atctcgtgtt ggtaaaccaa ttttgatatc atcatgaaca ttaacactct
    12901 caataacttt caaggtaagt ccattggctt cgacttcatc ttttaaagat ttaattcgtt
    12961 ctctcggcca aacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
    13021 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
    13081 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
    13141 aactgcttga ggacctacta gcatttcgca aaagattttt tcagttttct ttcctacccc
    13201 aacttgatat aaatctaaac caaagatttc tttattttct aaaatttcct gtaccgcaca
    13261 atgaatcttt gaaggatctt gttcacctaa tttcaatact gataatttat cctgcaactc
    13321 taccaataac ggatcaggac ttggcacaaa attttcccct tgatcatcga ctgccagtaa
    13381 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
    13441 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
    13501 tgttgcaatt cgttgtggtg tatctggaat atagggatta ggcaaacgtt tattaattac
    13561 ttcatcaata aattgttttg gatcaataat gcctggattt tcaactactg gtaaaccttc
    13621 taaataacct attcctttga ttaaaccaac caaatcatca tcctgcatct ctgctgaaat
    13681 tgattgatat cctagcagac aacctgttac tgccaaagca gtatgaagtg gattcaagct
    13741 tgtcgtaacc ttcatgatgt ctgctttatc aaccgtctct ctatctgtca taatcacacc
    13801 agccttttcc aatttaggcc ggccatttgg aaaactatct tcaataacta aataatgaac
    13861 tacctcagta ttagtgaaag cagcaatatt ggtatgtttt tttgtgtgaa tgatatctaa
    13921 atcctcgagc cccatcatca atagatcttc ctttacattc tctgaaggat ttggcgtaat
    13981 tcgatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
    14041 tttttcagaa acatatcctt ctttttgcca agcttctgca atcgtaaaaa tactatcttg
    14101 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gcaactctcc
    14161 agcattaaat ctgctatata acaaggatgt tacaatactc atcgtatgtt gaggattgct
    14221 aggtccagat ttgatatcag ctttgactac cgaaaagaat tgccctttag aatcttttaa
    14281 actgtaacct ttttcagtaa tagtaaacgt tgtaaactgt aatttaggat cttcaaaata
    14341 tttaaccact ctttgatagt ccgctatatt cttcggattg caatataaac tatcagccgt
    14401 tactgccaaa agtctttttt tgagttcacc atctccactc ataacaattt ccaaaatgtc
    14461 attgttatag tcatgataag ctttttccac tacttcttca tcaaatgttt cacacacaac
    14521 tactcctgtt tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
    14581 tcgataaaga tttcctccac caaaatgaag ccatactgga tgttgctggc cttcttgctt
    14641 aacttctttg acatcaaaac acggcagtag aatctgcatt tcattgaatt cttcgataga
    14701 attttgccaa tttttaatcg aaaccataat taacctcctc aaactgatat gttagttttg
    14761 atacatttta tattttaaaa cccattgtaa ccgttgtcaa tataaaaagg gaataaactt
    14821 gcttttttct accatatcag aatgattgta ttttttattc agaaaaataa aatactcctt
    14881 ctttttggac tgattttatc gaaacttatt ttttcttttt ttcatgtatt cttttatttt
    14941 tataactagt gtattacttc aaaatttgat attatatggc atgaacagaa ttaaaatatc
    15001 agttaaaata aaagttaagg agcattgtgt atgacaatta aaactagtct ccaaaatcaa
    15061 gcttaccaat ctattcgccg acaaattatc tatgctgact tagaacctgg aaaaaaagta
    15121 tcagaaaaaa aattagaaga aacactttct attggtcgta caccaatacg tgaagcactg
    15181 attcaactcc gacaacaagg tcttgttgat acaattcctc aaagcggaaa ttatatctca
    15241 ttaattgatt tagattcggc caaaaatgct cgatttgtca gagaacattt ggaacgacaa
    15301 attatgttag agtgttgtgc caaaatgaat aaccaaaaaa aacggatttt agaaactata
    15361 atttcagaac aagaaaaagc tgttagtcaa aaaaatgctc gtagtttttt tcataatgat
    15421 aatttattcc ataaagcttg ttttgaaatt gcagagcgag aagaagtatg gcaatggtta
    15481 gaaaataaca atacacactt agaacgattt cgatggctac gagtaaaaac ggaaggatta
    15541 aagtgggaaa aaattatgga acaacattat caactatttg aggcattaac aaaacaagat
    15601 ccagaagaag ctaactttct aacatcgttg catcttcatt taatgcttaa tgagcaaaat
    15661 attgttttag ctactcatcc aaaatatttc aaaaatatat aacttttaac tctttttaaa
    15721 ttaaatggtt gttctgatca gaaaaatatc gcttgttttc ttattatgta atatatttca
    15781 taaactattc tgcataacaa ctattgtttg tactttttac attggataac taattatgac
    15841 tgctgataac ttttgatatt aaactagtat ctccaataca aataaaaaat agatatcttg
    15901 ttcatcaaaa ttagttcgtg gatacttacc ttcatttaca tcattcagcg gtttcccatt
    15961 ttcttttatt ctattatttc attttctaat agacttggct tcatttcgca atactttttt
    16021 tcaataggca tcaaattggt gttgtttaga tatttagttg tagttttgtg acatgataag
    16081 tatgctcctt tttctctttt ccactaatac aacgatcaaa gggtgctttt ttcaaaaaac
    16141 tagaaaaatc aaaataagat tcgtattttg attctatata tcctgtttca actacatctt
    16201 atatttcaca atagaataaa tagccttttg catagctgct taacttataa gaaattgcaa
    16261 tttacttctt caaagtgaaa acgaatatga gtaaaataaa attattcttc ttacagataa
    16321 ttaaaaatag cgcgtaacaa aaatcggcat tcaatcacat aattaggatt gatgccgatt
    16381 acttatattc tacgtatagt tttagtattc ttatttttta tcaccagtaa ccaattgaga
    16441 acagccctaa agcagaatag tcttttatct caaaacgctt aatttcaggc ttaggaatat
    16501 gaataatctt ttgagtatat tctttctctt ttaatcttac tgaagaaata ccactgcc
//
LOCUS       DAYYBH010000054        16105 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000054 DAYYBH010000000
VERSION     DAYYBH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.056"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..362
                     /locus_tag="ACHAUG_002009"
     CDS             <1..362
                     /locus_tag="ACHAUG_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8481093.1"
                     /translation="SDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSST
                     TSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVIL
                     LVIGLRITFMSYKKSKR"
     gene            complement(517..1503)
                     /locus_tag="ACHAUG_002010"
     CDS             complement(517..1503)
                     /locus_tag="ACHAUG_002010"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8481094.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1526..2092)
                     /locus_tag="ACHAUG_002011"
     CDS             complement(1526..2092)
                     /locus_tag="ACHAUG_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8481095.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2106..2522)
                     /locus_tag="ACHAUG_002012"
     CDS             complement(2106..2522)
                     /locus_tag="ACHAUG_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8481096.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2979..4913)
                     /locus_tag="ACHAUG_002013"
     CDS             complement(2979..4913)
                     /locus_tag="ACHAUG_002013"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8481097.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5215..6150
                     /gene="pplA"
                     /locus_tag="ACHAUG_002014"
     CDS             5215..6150
                     /gene="pplA"
                     /locus_tag="ACHAUG_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8481098.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6240..7343
                     /locus_tag="ACHAUG_002015"
     CDS             6240..7343
                     /locus_tag="ACHAUG_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8481099.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7356..8309
                     /gene="menA"
                     /locus_tag="ACHAUG_002016"
     CDS             7356..8309
                     /gene="menA"
                     /locus_tag="ACHAUG_002016"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8481100.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFTMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9123..10469)
                     /gene="gor"
                     /locus_tag="ACHAUG_002017"
     CDS             complement(9123..10469)
                     /gene="gor"
                     /locus_tag="ACHAUG_002017"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8481101.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10603..11856)
                     /locus_tag="ACHAUG_002018"
     CDS             complement(10603..11856)
                     /locus_tag="ACHAUG_002018"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8481102.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11862..12785)
                     /locus_tag="ACHAUG_002019"
     CDS             complement(11862..12785)
                     /locus_tag="ACHAUG_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8481103.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13006..15498)
                     /locus_tag="ACHAUG_002020"
     CDS             complement(13006..15498)
                     /locus_tag="ACHAUG_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8481104.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15505..15972)
                     /locus_tag="ACHAUG_002021"
     CDS             complement(15505..15972)
                     /locus_tag="ACHAUG_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8481105.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4727 a         3361 c         2862 g         5155 t
ORIGIN      
        1 gctcagattc atctaccact acaagtgata gcacggacac gtcagcttca agtgacagta
       61 cagatacatc ctctaccaca agtgatagct cagattcatc aacttcaagc gatagtacag
      121 acacgtcgtc taccacaagc gatacacgtc atgcaagtgt ctccttctca aaaacaagtt
      181 cggataacag gacgaacttt aacttaacta ataaaacaag caatgacaat ggaaacaaat
      241 caaaaagagc acttcctaaa actggaagtc aatcgaataa ttggataaca ctcgcaggcg
      301 ttatattact tgtaatcggt ttaagaatca cttttatgag ttataagaaa tcgaaaaggt
      361 aaaaaaatat tcaagataaa aaagagcaac gtcgctaacg gacgttgctc tttatttttg
      421 ctttttatca taaatacaag aactacaatt caatttgtca aaaaacacgt agatttcctt
      481 ctgagaaatt tatttacgta tcgaaccttt ccctattcag tctttgcgat ccaaaatcag
      541 ttgggtaagg gaaaagagat tctcttttgt tcgaagagag gtggcaggta atttctttat
      601 ttccttcaaa gccttttctg tgtaagaggc cgctaacttc ctagcttctt ccacgcctcc
      661 tgtatgttca acgattttgc gtacggcgtc gacatcttct tctgtcattg ccgctttctt
      721 ctctaaataa ggtagtagct ttggtttgtt tttttgcaaa gagtagatca acggtaaaga
      781 atagacccct tgacgcatgt cttctagtac aggtttgccg atttcctggc tctcttttgt
      841 ataatctaaa atatcatcga taatctgaaa ggccatcccg atagaaagtc cgatatctcc
      901 tgcttttttt gcgaagcgct cgcttgttcc actttcaaaa gcgccaacag aacaacttaa
      961 ttggaacagt tcagctgtct tacctgaaat attttccaaa tattctttta cagaaagttc
     1021 ataattatag cgatcatcca tctgccctaa ttcgccattc aatacttttt ccatactgcg
     1081 agaattcaat tggagacttt tcaaggaagt ggagtaatca gacaatagtt taaaacagca
     1141 gacaaataaa tagtctcctg catagacaga aacgacatta ccaaactgac tacggatatt
     1201 aggctgacca cgtcgcagat ccgcttcatc aacgatatca tcatggatca gtgtggccgt
     1261 atggagcatt tcgatagaag ctgccaaagc gatggctttg tctcgatctt gctctggtcc
     1321 aaattgtgag aacaatagct gataggcagg acgcaacagc tttccgccgg catggatcgt
     1381 ttctttgatt gcttgttcga caggtttgtt tttcaggcgg acagtttctt ccatccgttt
     1441 tagtgtttct gtcaattctt tacttaaagc aggatagttt tcccacattg gatgtagctt
     1501 cataaaaaaa ctccttgtgt gacgattatt ttttggtaaa cattgggctc catttttgtt
     1561 tggttccctg ctcataatct ttttggaatc gttgaagtgt ctgcacatgt cgtaaaagat
     1621 aattcgctgc gatcccgatt gcaatccctg ataaaatccc taaaactgaa aggactggca
     1681 agtacaacat gactgtccat gattgtgcaa tgaaagaagc catcaaaagc tgtccgacat
     1741 tatgcatgaa accgccggcc gcactaattc cgatgatact gacacgtttt ggtcctaatt
     1801 gcttgaccaa taacattcca aaataactaa gcaaagcacc actcatacta taaaggaatg
     1861 tggaaatcgt tccgcctagc aaagtagtca ggaatagtcg tagccaaagt agtaagaagc
     1921 tgtcttgttt tttcatggta aaaatggcaa cgatcgtgat tagattggct aagcctagtt
     1981 ttgcaccagg cgcaaaagaa aaaggataag ggatcatatt ttccaataaa ccaatgatca
     2041 ctccttgtgc aacaagtagc gaaatataaa tcattttttg taatttactc atggtgtgta
     2101 aaggtctaat aaatcagact gtcttcgtca cctccatctg ttgaacggac ttcgatcact
     2161 aatttatgtg gcaaacagac gatggtttcg ccatttttcg ttgcccagcc tcgacgtacg
     2221 cagatctggt catcgcaatc tgcttctttt atccgtactc gatcgccatc tatctcgatc
     2281 aagttataat ctccgtgttc atctttgtaa gtatatgtgt tgctctcttc tccagccatc
     2341 aaagggaatg ttttgatttc tgtgccatcc acacgcagta ccgcttcttt ttctgctgtt
     2401 gcggtgtttt gctgaagacc aaaaatcatg actggtaaaa aagaaagaac agtcaaaagc
     2461 aaaataataa aaacatccca aggtttgagc agactttttt cagcaaattc tttaaggctc
     2521 atttttgttc tcctttttcc agattaactc acccaatttt agcataaaaa caagaggttg
     2581 tgacacctgt gtcttgcttt gagtaataag aacgaagaat agaaaacagc tttccatgtt
     2641 ttctaattct ttgggcttat taccgagaag cagcaggtgg aacaccgttt ataacaagag
     2701 gttgtgacag aagtgggcaa cttcaagaaa taagaaggaa tttacgaaaa ttacttttca
     2761 agtttttgtg aaatttcggc ttatttttcg aagaagctac ttctggaaca ccgtttataa
     2821 caagaggttg taacagaggt tgtgacacct gtgtcttgct catggaggct tatctcgggc
     2881 atcttattcc tttaatttta cataaaaagg aggctgtgac ggtttgtctc agcccctttc
     2941 tattgaaatc ttgcattcaa ataacgttct ttatctattt atttcttgcc gtactgggaa
     3001 cttggtctac cgtaccacca attacctagt gtacgttgta gccatggaat tacccagcga
     3061 tccaacccaa tcgcacgtcc ggaaccgttc atcaatgcga aagcaacaaa aatgaaccaa
     3121 atgtttaccc agtaaaacat gcctgataag cagaacgtga tcaccaatac aatcgttgtc
     3181 gcactgcata gccatgtgaa taatccagca atcaatgcta atgccaaggc aatttcaaag
     3241 aataccatga atttttggaa gaacattgct acatcattgt ttggcatgat cacttgcatg
     3301 atctttgtga accagtctgg tgacttctca atcacagcca ttggttcttc accgtatgca
     3361 taactcaaac caaaatgtgc agcttgagtc gctgcttctc cggcacttgc ccctgctccg
     3421 ctggcagctg tggttgcatc accagcagca gaagcaccac tggcaacatc tgttacattg
     3481 tattcccatg tccaagggaa cacactgttt ccagtgaacc atgatccttc accaaaccaa
     3541 gtacttggtt tgaacacttc accattccca atgatttttt tggaagcttc aacgaaccaa
     3601 actaaaccat agaaaatacg aagtggcaca ctccataaga cattgccgta acgagaagaa
     3661 tgtcctcgag tcacgttgcg atcatctttg atatggaaaa actcatgcat taaatattgg
     3721 aacatataat aaccagaacg aatatcaaag aagtatttca aattcacgat atgtttcatt
     3781 acgatagcta aaaatccgct taaatggatc ttatcaaata ggttagctac cccccatttg
     3841 gcaccaatag aaaccataaa tccttgatag tttcctttaa atttatgctt ttctccgcct
     3901 ttgatcgaag ccacgatatt cgctgcggct gtgtgtcctg tttgttcagc agcttgaacg
     3961 atctgcggtg tcggtgttcc tgggaattct tcataaaaga ctaaatcacc aatgatatag
     4021 atgtttttat cttcgtagcc ttttgcctgc atatattcat tggcgatcaa ccgctgtccg
     4081 cgtgctgatt ctaatccaaa gtcagcagcg tcagaagttc ctttgacacc agctgtccaa
     4141 atcagtgtgt gagtcggaat gttgcgtccg tctttcaatt tgatatgatc agcagccact
     4201 tctacgattg gtgcattcaa taataattcc acgttgtgct tacgcaaata gcgttcagct
     4261 tttgcagcat catttcgatc caacatattc aagatcgtcg gcattgcctc tacgaccatc
     4321 agtgtgattt cgtttggatc taatttataa tctttggcta ggcgattttt ccagtcgatc
     4381 agttccccaa tcatttcaat accggtaaat ccagaaccac agacaacgaa agtcagcatg
     4441 gctttacgca gttcatcatc tggttccaat gcagcttttg ctactgtctt ctcaatatgt
     4501 tcccgaatcc ttacagaatc ttcaaatgac caaagggtga atccgtgtgc tttgacacca
     4561 ggtgtaccaa agtcattcgg ctctccgccc attccaagga tcagctgatc aaaaggatag
     4621 gaacctaatt tggtcttgac gatttttcgt tctttatcga taccgattac agtgtctgtg
     4681 acaagttgta cattcttttg acgagcaaac aaatgctgca gatcgtattg gattgcttct
     4741 ggttctacac gtccgccagc tacttcatgc aactcggtca tcatagtatg gtacgaatga
     4801 cgatcgatca gagtaattgc tacatcttct tttttcagtt tctttgccaa gtattttgta
     4861 gctgaaacac cggcatatcc tgctcctaca acgacaatat tttgctttgc cattgatcat
     4921 ccgctcctta tagactatct atataatgaa tatatgataa ttcttttagt ttgatactta
     4981 ttacacaatc tccatatatt atatagatgt tctgttcttt tgtctaactc cttttggatt
     5041 tttttcaaat tttaaaataa agcgtttttt cacgaaattt gctgaaagaa aacgtttgaa
     5101 atattacatg ggttatttat caaaaatagt tgacttatag gctaaaaaga ttagaatgta
     5161 taatgaatca caaagaaaaa ttgtggacta gaaatggaat ggtggggtat taaaatgaag
     5221 aaaaaacgtt ttgtaactgg atttgccgta ttagcatttt cagctctagt gttaggcgct
     5281 tgcggcgcag atgacaacgg aagctcaaac tcttcttctg aatcatctac agcacaatct
     5341 tcgactgcaa aaagcacaac agaatcatct gcaaaagttg tagctggcgg agatttgcaa
     5401 gacggcacat ataaattaga agaaaaaaat tattcaaatg gttaccgtgc agtctttgaa
     5461 atggttgtaa aagacggcaa aatcactgaa tcaaaatatg ataacgtcaa tgaaaacggc
     5521 gaatcaaaaa caaaagatgc agaatacaac aaaaacatgg aagcaaaatc tggcacaaac
     5581 ccagaaaaat acattcctga gttgaatgac caattcttaa aagcacaaag cgcaagcggt
     5641 gtggaagtcg taactggagc tactcactca tctgaaagct tccaaaacta cgcacaacaa
     5701 ttgatccaag ctgctcaagc tggtaacaca gatacaatcg aaatcgacaa tggtgctgat
     5761 ttgaaagacg gcacttacaa gcttgaagaa aagaactact ctaacggcta ccgtgtgcaa
     5821 ttcgaaatga cagttgctgg cggcaaagta actgaatcaa actttgatta tatcgacaaa
     5881 gacggtaaat caaaacaaga tgatacagaa tataatgaaa atatgaaagc aaaaagcggt
     5941 acagaaccaa aaacttacat ccctactctg aacgacgaat tcgtcaaagc aatgggcgaa
     6001 gaagatggct cacctgctaa tgtagaagtt gttacaggtg ctacacacag ctcacattca
     6061 ttcatcatgt acgcacaaca attagtgaat gcagctgaaa aaggcgacac tcaaacaatc
     6121 gaagtagata atatcgtaac agaaaaataa tcacataatc atactaataa aagttggagg
     6181 gtgggacgat agccagatta ggcagtcgtc tcacctttca aaatgaagag aggaaaaaga
     6241 tgcggaaaaa actactattt tccttaatca cactgggcat ctcttttatg gtgctaactg
     6301 gctgcagcaa ctcagcaaat aaaacaacca ctgaaagttc agcagctacc aagatattga
     6361 aagatccata ttctgatgaa cagttcttgt tagggactta cgttcgtatc cgtgtgtatg
     6421 atgaaggcaa agaatcagca ttgaaacctg ccttcgatcg agtaaaagaa cttggtgata
     6481 aaatcacgat caatcaaaaa ggttctgaga tcgatgaagt gaatgagcaa gctgggatca
     6541 aaccagtaaa agtttctgat gacgtctaca cgttagtcaa gcgggcctac gaatatagcc
     6601 aagattctca aggcggattc gatatggcga ttggtgcaat cacacaatta tggcgaatcg
     6661 gttttgatga cgccagaaaa ccttcccaag aagaaatcga tcaggcattg aaattagtgg
     6721 attatcataa gatcgaacta aatgacaaag aaaaaactgt ttatctaaaa gaaaaaggaa
     6781 tgatcatcga tttaggtgca atcgccaaag gttacattac ggacgaagta gtgaaagtct
     6841 tgaaaaaaca aggagtgacc actgccatcg ttgacttggg cggtaatgtc tatgtcttag
     6901 gccacagccc aagaggcgaa aaccaagact ggacagtcgg catccaagat ccgaacatgg
     6961 ctcgcggatc tgttttaggt tctattaaag aacggaacaa aacattagtt acctctggaa
     7021 tttacgagcg ttatttagaa gtagatggca aaaaatacca tcatttgttt gatccaaaaa
     7081 cgggttatcc gttcgacaat gatatcgcaa gtgtcacgat cataacagat aaatcaattg
     7141 atggtgacgg gttgtctaca gcagtctttt caatgggtgt gaaaaaagga ctggaatacg
     7201 tcgaaagcga attgaataac gggacggaag cgatcttcgt gacaaaagac gataaagtat
     7261 atgtgaccga tccaatcaaa gacaccttca aattatcgga ggactcccat tacacgatgg
     7321 gtgaccgttc tgaactaaaa taaggagtac gaaccatgac actaaaaatc tttttagaag
     7381 tagttgaaat acgaacaaag gtggctagtg tatttccttt tgcggtaggg gtattattct
     7441 ctatcgcttt ttttcatgag atacattggc taaacacttt tttatttttt attggaatgc
     7501 tagtttttga tttagcgact accgctatca ataattacat ggattatgaa aaggctcatt
     7561 ctgaagtata taagtatgaa gaaaatgtca ttggccgcag aaatctatct cctgtattga
     7621 ttcgaaacat gattttagct atgattgcat ttgtttttgt tatcggcgtt attctaacga
     7681 tcaatacggg ttggctttta cttttgatgg gatttatctg ttgttttatt ggagtattct
     7741 atacattcgg accgattcct ttatctcgaa tgcctttagg ggaaattttc agtggggtga
     7801 ctatgggcct tggaattttt accatggtcg tttatgtcaa tacattcggt tcagatgtct
     7861 atgatctgac agtcaacttt tcatctggac aatttgcttt ggtaggaaat ctttgggcta
     7921 tagcagcaat cgtattagct tccttaccct tggtgtttac gattgccaat atcatgttgg
     7981 ctaataattt gcgggatctt gaccgtgata ttgaaaatca tcgctataca ctagtttttt
     8041 atatcggacg tcctgcggga acagtcttat tccagctatt gatgtatgct tgctatttcg
     8101 tgattttaat ggggttgttg agcggtatct atgaatggcc gatcttggtc accttcttga
     8161 ccttgccaat tgtgaagaag catttggatc aattcaagca aacgttaccc cagcctatca
     8221 gtttcagcta ttccatcaaa aatatggtct tattcaatgc cagttatgta ttggggctat
     8281 tactaacaat cattatgcaa gccatttaac agcatgaaga ggttgtgaaa aaactgccct
     8341 gcatcaagta ataagaacag ccaaataaaa atagctcctt atatttttcg tttggttggg
     8401 cttattaccg cagatgcaag cttttgaaca ccgtttatca ggaggttgtg aaaaagtcgc
     8461 ttagtttcga gtattaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     8521 tggcttaata ccgagaaact ggcttttgaa caccgtttat cagaggttgt aaaatcagtg
     8581 ttttgtcctg agtattagag aaacaaatgg aaaaatagtt cctcatattt tgccgttttt
     8641 tgagctaata ccgaaggaca gctgattgaa cactatttgt tcggaaaaag tgactaacta
     8701 tgacacaatt tttttcacag tccctttttt cacttgattt gattcgtacc agcgaacttg
     8761 acgagaactt tcttggtttg gtaactatat ggtattcttt taacatcctc gtttgcttct
     8821 tctaatttat ttatacaaag tcacactttt ttaaacatct gttcttcgat acatttctat
     8881 ttcttttaaa catcgatacc aatattttga acttaagagt gcataccaaa tggattcatt
     8941 tactcttcgt cagttacgaa gataatcaaa atctccaact tccctactta tttcgttttg
     9001 agaattatcg aagcaatctt acataaataa ctaaaaaaca aaaaaccaga aaatcagtat
     9061 actgattttc tggttttaca tggtttgttc aataaaaagc tgttggaact gttaatatta
     9121 aattatcgca tcgttacgaa ttcttccgct cctgttggat ggatggctac tgtattgtca
     9181 aagtcttctt tagttgcacc catcttgata gccacggcaa aaccttgcag catctcatcg
     9241 acaccaacac caatagcatg taagccgacg attctttctt cttttccgac acagatcaat
     9301 ttcatttcgc atttttggcg ataatcattt aatgcaaagt acattggggt aaagcgtgaa
     9361 cgataaactt tcaattcatt ttcgccatat ttttccaatg cttcttcttc tgtcaaacca
     9421 attgttgcaa ttggtggatg cgtaaagacg actgtcggaa caagtttata gtcaagataa
     9481 gaatctttct ttccattaaa taaacgttct gataaacgtc taccagctgc gattgcaaca
     9541 ggagtcagat ctaatttacc gatcacatca ccaacagcaa aaatatgttc atccgttgta
     9601 ttttggtatt tatctacttt gataaaccct tttttgtcca attccacatt tgtattttcc
     9661 agacctaatt tttcggtatt cggtacacgt ccaccggcaa aaagtacaca atcaccagtt
     9721 aaggtttcac cattttcaaa tagcaccgta tattcgtcat tgtttttagt aatttcttta
     9781 gcaatatgat gtgcgtaagt ttttattcca gcttcttggt acatttggat caaattgtcc
     9841 gaaagcattt tatcaaacgt tctaagtggt cgttcttttc gatatgccca catgacttca
     9901 ctacctaaac cacgaaatac acctgaaagt tcagccgcaa tgtatccagc acctaaaacg
     9961 attgctctct taggtaactc tgttaattca aaaaatccat ttgaatcaat tgcgtattct
    10021 ccaccaggaa tggtcatttt acttgcacgg cctccagtag cgatcaagat tttaggagca
    10081 cgataggttt caccgtttac ttcgatcgtt tgactatcga taaaagtagc gtaacctttt
    10141 atagcctcga catgattgct atctaatcct ctttgataag caccgtgtag aaaatcaata
    10201 tatttttctc gtctttctac tagaccggcg aaatcaacat tttttaaatc tgcttggata
    10261 ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg tacttgcttg ccacataact
    10321 tttttaggta cacaaccgac gttgacacat gttccgccca attcattcgc ttcaatcagc
    10381 aaaacttttg cgccatgcat ccctgctctg tttgctgaag caataccgcc agagccgcca
    10441 ccaattacga tgtaatcata tatttgcatt ttgttcctcc ttattgaaga gatcaatcaa
    10501 aacctttatt taacgatcct ttttctacag aaaacaagtg taacactttt cttgaatttt
    10561 gtatgcgaag atgcttaaaa cagggatata tcagcttttt ccttatttac gttttcggat
    10621 catatctcca ggtttcaccg gctgtgcgac tggcatagtt aaaatcatca ttggatttgg
    10681 tgctcggtca atggattcgc cttcttcatt atgcataact tcaacggttt gactgaaatg
    10741 actgaatcct ggaccataaa attcgatttc atctcctaca gaaaacaagt ttctctgacg
    10801 tatcgtagca atctgtgttg cctcatcata agataatact tcaccgacaa atttatatac
    10861 tgggatttta cgacgttcac cgaataattg ttcgttttca gttggtgtat tgtagtaaaa
    10921 acctgtagct aattctcgtt gtgctacttt ccataactcg tcgatccatt cttgcttgca
    10981 gacatagttt tcaggatctt ccatataact gtctacagcc gctttataga catttgacac
    11041 cgtagataca taatggatcg atttcattct tccttcgatc ttgaagctgt ctacgccgtt
    11101 ttcaatcaat tctggaatat gttcgatcat tgacatatcc actgcactca tcgagaattc
    11161 ttcttccact tcgcctgtat cagttaaact gtttcgttct tgaccaaaag gcatatcgaa
    11221 caatccatac ttccagcgac aagattgaga acatccgcca cggtttgcat cccgcatcga
    11281 catatggttg gacaatgtgc atcgaccaga ataagagata cacatggcac catggataaa
    11341 tgcttcgatt tcggtatccg tatttttacg aatctctgcc acttcctcca ttgaaacttc
    11401 acgagctaaa acgacacgtt ccaacccttc ttctttccag aattccaatg tctcaaaatt
    11461 agttgctgaa gcttgagtgg ataaatggat cggcagacct ggggcttctg tcgcacaaat
    11521 ttcgatcaaa gctggatcag aaacaatcac tgcagaaata ccgacatcac gcaaactgcg
    11581 gaaaaattcc cctgctccat cttcatttcc ttcatgggtg accatattag cagcaacgta
    11641 taccttcgca tcatgttctt ttgcaaaagc aactccttca gccatttctt cataagtaaa
    11701 attacctgca cggcttctta aaccatatgc attcccgcca atatatactg catctgctcc
    11761 ataatggatc gcggttttta acttttccag tgttcctgct ggcgctagga tttctggtcg
    11821 tttcaatttt ctttttactg tcatttcttt catcatactc cttatttgat agcagaagga
    11881 tcaatataaa agaatcctgt atctaattca cggttttcag gatgcaattt actgatttcg
    11941 ctagtcattt tttccgcttt ttcttgatcc cagctacctt ctaaaatttg tttttttgct
    12001 tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg gagtaaataa tccgtccaat
    12061 ttccatgtac agtaatgatg ctgataaagt tcattcaatt cattcgagag attcagatca
    12121 ttgctggcaa aaatatgtgt gccatgacta tcttcataaa tagaataatg tgtctcttct
    12181 tttttaggtt ccgagataaa aagacctctt tcacgatctt tttgttcgtc ttgtttcgta
    12241 tagttatagt aattctgtag cagtggacgt ttggattgat ggatgcatgt agcaccatac
    12301 acaagggttt ctacaggaat atcgagtttt tcttccatcg ccttcatctc ttcaaaagga
    12361 acttcacgag caagtactgc tccaactgcg ccttttttcg cccagaaatt gatttgacgt
    12421 gcgctcgtta ctaatgtttc agcatcataa atatacggta tacgcaattc aggatttttt
    12481 ttcatcgtat aaataatacc tgtatcacca actgtgatca catctacgtt tatttctttt
    12541 aaaaaagcca aatattcagg tacatttttc attttttcag gatgaatgat cccatttaca
    12601 gcaatattcg cttttttacc atacttgtgt gctaattcta ccaattgtcg ctgttcttct
    12661 cgtgtaaatg aagcaggcag acgcaatcca aattgctctt caccgaaata tagtgtatct
    12721 gcacctgcag caagcaactg ttctgcttgt tcaatagttt ctactgtggt tatgagttcg
    12781 atcataattt tctcctccta caacttcata atagtagcat agctctaaaa actttccaat
    12841 acttcttcta tttaaagtcg gatttttgaa aattatttca caaaaaatca aaataaagtt
    12901 cgatatattc tgtccaaata taaaaagaga ttgtgacaaa aatcttgtca caacctctta
    12961 atcaagtctt actattataa aaattcagct acacaggcca agtaactata gggaacctac
    13021 tgtttccttt ttaggtccgc gaacattcaa tgtgatctta ccttttgaag cacctactgt
    13081 tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc tcgctcaatc gatcttcaat
    13141 ttctttttga agtgcgcgac ggattggacg tgcaccatat tcaggatcaa atccagcttt
    13201 cccgatcaca tcgattgcag atggtgtgat tttcacttga atatcttgtt ctttcaatcg
    13261 tttgataatc gctttgctca tgatttggac gattgtatgg atttcatctt tactcaatga
    13321 atggaagacg acagtctcat cgattcgatt caagaattca ggtcggaaag ctttctttaa
    13381 ctcttctaag atacgttttt gcatggcttt gtgatcttta gtaacatctt ggacattgaa
    13441 tccgacattt ttttcttctc gaatttgtgt cgctccgata tttgaagtca taatcatgat
    13501 tgtatttcta aagtccactt ttctaccttt agaatctgtt aaatgaccat catccaatac
    13561 ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc acttcatcta agagaatcac
    13621 tgaatatggt ttttgacgaa ttttttctgt caactgtcca ccttcatcat accctacgta
    13681 tcctggagga gacccgatta atcggcttgt actgtatttt tccataaatt ctgacatatc
    13741 aacacggatc agggcatctt cacttccaaa catgacttct gaaagtgctt tggccaattc
    13801 tgtttttccg acaccagttg gtccaaggaa catgaatgaa ccaattggac gatcaggatc
    13861 tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact gctttgacag cctcatcttg
    13921 accaacgaca cgttcatgca gaagtccttc cagttctaac aagcgttcgc tttctttttt
    13981 ctccaactgc tgtagaggga caccagtcca ctccgataca actgtagcaa catcttcttc
    14041 tgttacacga tcagaataac cttgctcctg ttttgcttca attgcagaaa ctttttgcag
    14101 ctttttacgt agtgcttttt cttgtctgcg taattgagca gcattttcaa agtcttgttt
    14161 ttggatcgct gcttcctttt cttgtactag ttcatcgatt tctagtttga tcacagttga
    14221 ttttgttaag tgatcggttt gatccaaacg gactttcgct gctgattcat cgatcaaatc
    14281 aattgcttta tcgggtagtt gacgagaagt gatataacga acagaaagat tgactgctgc
    14341 tcgtacagct tcatctgtga tctccactcc atggtgctct tcatatcttg tacgtaatcc
    14401 ttgaagaatc acttctgctt cttcaggagt tggctcatct acttgaatgc gagcaaaacg
    14461 tcgctctaat gcagaatctt tttcgatata tttttgatat tcatctaggg tcgttgctcc
    14521 gatcgtttgt aattctcctc gagctaacgc cggtttcaag atattcgaag catcaattgc
    14581 tccttctgct ccacctgcac caattaaggt atgcaattca tcaatgaata agattacttg
    14641 accatcatta tagatttcgt cgatgacctt tttcattcga tcttcaaatt caccgcgata
    14701 tttcgtacca gcaaccaaag atcccatatc aagcatcatc aggcgctttt cttgcatgtc
    14761 ttctggcact tcacctaaaa tgattttttg agctaaccct tcagcaatgg ctgttttgcc
    14821 tacaccaggt tcacccacca gtacagggtt attctttgtc cgtcggctaa gaatctggat
    14881 aagccgtttc acttctctcg aacgaccaac aactgggtcc agcttgtttt cttttgctaa
    14941 tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt ccttgcggtg tttgccgttt
    15001 atcttgtaca tttacatttc tacgacgatt cattcctttt ccagcagaat caccagctag
    15061 ccccattttt tgtttcagaa cttgacgggc ttttgaaagg ctgataccta agttgatcat
    15121 gattcgagta gccatgatac tttcatcttt taataagctc atcaaaatat gttctgttcc
    15181 aactttttga gaaccagttt tcttcacttc gctgctagct aaagcaaaaa cttgtttcat
    15241 tcttggtgaa tatggaagaa agactccttt agggtaagaa cggataccac cataaccaat
    15301 cagatgttca atttcttcat aaatttccgt ttcattcaaa tccatttcac gtaatacttt
    15361 tccagcaatt ccattttgct cgaccactaa ggctagtaaa acatgttctg aactaactga
    15421 ttgatgtttc aaccgttttg cttcttcttg tgcaatttct aatactgctt gggctctttc
    15481 agtaaaaagt tcttccatct aatctcactt cccatcttca taacttaaac gttccagaaa
    15541 tgctcggaga atccgagcac gtgtatgatc ttcatattca ttgaaattca atgtgttttt
    15601 tgcaattgca ctcaacatca agtttccttc tttttttgta attatttggt cttcataaag
    15661 tttttgaata atagcaaaag catttttctc actgattgta tcatcaaata actgatttat
    15721 ctgctcaagt aattgttttt tatctgaaat ccttactttt gcaattcgta tataaccacc
    15781 accaccacgt ttgctttcta ctagataacc acgttggatg gtaaaacggg tattaatcac
    15841 atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc atttcaatcc ttcgaatctc
    15901 gatcatttca ctattttcca gaattttctt tatatatgat tcaattaaat ccgaggtatt
    15961 ctgatgtgcc atcttattca ttccctttga ctaatattga ccttaattat aacgaatttg
    16021 ataatagaaa gaaagaagaa cgctttttgt taaataaatc ctttagaatc aacaaaaaaa
    16081 aatacggaat atactattcc gtatc
//
LOCUS       DAYYBH010000055        15741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000055 DAYYBH010000000
VERSION     DAYYBH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.057"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACHAUG_002022"
     CDS             complement(24..722)
                     /locus_tag="ACHAUG_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8481106.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACHAUG_002023"
     CDS             complement(715..2067)
                     /locus_tag="ACHAUG_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8481107.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACHAUG_002024"
     CDS             complement(2290..3690)
                     /locus_tag="ACHAUG_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8481108.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACHAUG_002025"
     CDS             3851..4588
                     /locus_tag="ACHAUG_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8481109.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACHAUG_002026"
     CDS             4713..5192
                     /locus_tag="ACHAUG_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8481110.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACHAUG_002027"
     CDS             5189..5956
                     /locus_tag="ACHAUG_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8481111.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACHAUG_002028"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACHAUG_002028"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8481112.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACHAUG_002029"
     CDS             complement(6859..7461)
                     /locus_tag="ACHAUG_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8481113.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACHAUG_002030"
     CDS             7567..7797
                     /locus_tag="ACHAUG_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481114.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACHAUG_002031"
     CDS             7788..8036
                     /locus_tag="ACHAUG_002031"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8481115.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACHAUG_002032"
     CDS             8142..8726
                     /locus_tag="ACHAUG_002032"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8481116.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACHAUG_002033"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACHAUG_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8481117.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACHAUG_002034"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACHAUG_002034"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8481118.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACHAUG_002035"
     CDS             10627..10806
                     /locus_tag="ACHAUG_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481119.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACHAUG_002036"
     CDS             10828..11853
                     /locus_tag="ACHAUG_002036"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8481120.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACHAUG_002037"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACHAUG_002037"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8481121.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACHAUG_002038"
     CDS             13425..14372
                     /locus_tag="ACHAUG_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8481122.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACHAUG_002039"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACHAUG_002039"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8481123.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACHAUG_002040"
     CDS             complement(15274..15516)
                     /locus_tag="ACHAUG_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481124.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACHAUG_002041"
     tRNA            15637..15709
                     /locus_tag="ACHAUG_002041"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4374 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt t
//
LOCUS       DAYYBH010000056        15732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000056 DAYYBH010000000
VERSION     DAYYBH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.058"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..261
                     /locus_tag="ACHAUG_002042"
     CDS             94..261
                     /locus_tag="ACHAUG_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8481125.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(323..2134)
                     /locus_tag="ACHAUG_002043"
     CDS             complement(323..2134)
                     /locus_tag="ACHAUG_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8481126.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2131..2496)
                     /locus_tag="ACHAUG_002044"
     CDS             complement(2131..2496)
                     /locus_tag="ACHAUG_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8481127.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2659..3054)
                     /locus_tag="ACHAUG_002045"
     CDS             complement(2659..3054)
                     /locus_tag="ACHAUG_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8481128.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3072..4034)
                     /locus_tag="ACHAUG_002046"
     CDS             complement(3072..4034)
                     /locus_tag="ACHAUG_002046"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8481129.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4034..4246)
                     /locus_tag="ACHAUG_002047"
     CDS             complement(4034..4246)
                     /locus_tag="ACHAUG_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8481130.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4267..4965)
                     /locus_tag="ACHAUG_002048"
     CDS             complement(4267..4965)
                     /locus_tag="ACHAUG_002048"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8481131.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4985..5527)
                     /locus_tag="ACHAUG_002049"
     CDS             complement(4985..5527)
                     /locus_tag="ACHAUG_002049"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8481132.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5659..6663)
                     /locus_tag="ACHAUG_002050"
     CDS             complement(5659..6663)
                     /locus_tag="ACHAUG_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8481133.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6660..7649)
                     /locus_tag="ACHAUG_002051"
     CDS             complement(6660..7649)
                     /locus_tag="ACHAUG_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8481134.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7646..8452)
                     /locus_tag="ACHAUG_002052"
     CDS             complement(7646..8452)
                     /locus_tag="ACHAUG_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8481135.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8618..9574
                     /locus_tag="ACHAUG_002053"
     CDS             8618..9574
                     /locus_tag="ACHAUG_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8481136.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9651..10169)
                     /locus_tag="ACHAUG_002054"
     CDS             complement(9651..10169)
                     /locus_tag="ACHAUG_002054"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8481137.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10257..10406)
                     /gene="rpmG"
                     /locus_tag="ACHAUG_002055"
     CDS             complement(10257..10406)
                     /gene="rpmG"
                     /locus_tag="ACHAUG_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8481138.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10634..11080
                     /locus_tag="ACHAUG_002056"
     CDS             10634..11080
                     /locus_tag="ACHAUG_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8481139.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11275..13170)
                     /gene="asnB"
                     /locus_tag="ACHAUG_002057"
     CDS             complement(11275..13170)
                     /gene="asnB"
                     /locus_tag="ACHAUG_002057"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8481140.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13495..14469)
                     /gene="bsh"
                     /locus_tag="ACHAUG_002058"
     CDS             complement(13495..14469)
                     /gene="bsh"
                     /locus_tag="ACHAUG_002058"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8481141.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(14803..14890)
                     /locus_tag="ACHAUG_002059"
     tRNA            complement(14803..14890)
                     /locus_tag="ACHAUG_002059"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14856..14858),aa:Ser,seq:cga)
     gene            complement(15035..15601)
                     /locus_tag="ACHAUG_002060"
     CDS             complement(15035..15601)
                     /locus_tag="ACHAUG_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8481142.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         4893 a         3289 c         2631 g         4919 t
ORIGIN      
        1 tttttgcaaa ggtttacaaa caaacgtaaa actagccttt ttttctcttt tgttccataa
       61 tagatataac aaataataaa tgaggtggat tcaatgaaaa aatttgtatc tggaatatta
      121 gtaggaagtc tggcaacagc cgctgccgtt gctggtttgg ttgcttctgt gaaaaaaaca
      181 gtgatcgatc caattgacga aaaagaagca atgatcgaag aaaaccgtaa aaaagcaatg
      241 cgtaaacgcg tttctcgtta ataattattt aaaaatacgc atgaagactt tctaaaaaac
      301 agataagtca tcatgcgtat ttttatttgt cagtcaatat aagttgtgct attttatctg
      361 cctgtctcag attccatgga tctgtataag gtctagctac tgctgcagtt agccaatcca
      421 caccttcaac aggtattcca tagcaataaa agtcagactg gatttcccct gaagcatcaa
      481 tcacgcgatt cgtacctcgc tcaacatcta tagcttctga atgataactt cctgtattat
      541 ctgaaaatga aaaagggcga acaagtttgt tttccttcaa attttttatg acaggattac
      601 ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat caaatagcga ctgctgattt
      661 ctttatcttg tagagaaata acatagcggt tgtgcttttg ctgaatatca aatggcggtt
      721 cgatcaaatg aaaaataccc gcatctatca gtgctgctaa ttctttcgtc cgaatgattg
      781 gcggaccgat cgttaaaaag ctatttagat gagtaaacca tccccatagt atttctttgt
      841 attccttagc agaaaaaacg tgccattcaa tagcctgccg gattggatca cgccagtctt
      901 ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt gcctttttcc gcttctttta
      961 tctgcccttc aataaatatt cggctagctt ctgaaaaggg ctcattctga taggcaagag
     1021 gagtttctaa atacgaccaa tcccatctat aagggattag tgctggatgc tttccgagac
     1081 actcctctga agagagggag agaaaatcat gctcaaaatt tttctttgga atcggcaagt
     1141 gatattcttc gatgattttc ttgtaataaa acaactcaac gtcttttttc agatagtcaa
     1201 aaaatgtttc gccagtcaaa aatcccgact gatgccattg ctccaggttt tcatttgtta
     1261 gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt tcttcctcga gggtaataag
     1321 ggagccccct tcttgatcca ctgtatacaa tcggttcctt gcctgatgga tgatagacca
     1381 gttttccctt ttcgttctcg aagcgccctc ctcttgatac tgtaaacaat ccgacataat
     1441 caaaaaaact taagcctaga cctcttaaaa agacattttc tttaggcggt atcttcgaaa
     1501 gatctacatc tgcaggatta gaaggaagct gatacagtaa aacatttttc tttgcgtagt
     1561 ctacccacac tctttcttct tctgtctcat cattttccca gtgaccgctt gctaaaacaa
     1621 gctgatcgat agaatagctt tgctgatctg tctgtaaaag ataaccatct tttcttttgc
     1681 ttactttttt tactaaagaa taaacaaact gaatagaagc tggaaattct tcttgcaact
     1741 ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg agtagattgc tgatcttttt
     1801 ccagacgatt gatttcttct ataattagtt gcttattttt ggttaccgtc tgttcgatat
     1861 aatctgtagc aggtccttta ctccattgaa acaagttggg tccctgtgtt accaatcctc
     1921 cactggacaa tgtttcatct gtaaaaagcg tcacttgttg agaaacagaa ttcattaata
     1981 actccggtgg ctgattcaac cgccaaactc gacctccagg accattggga tcgaaaagaa
     2041 aaagttcaag agaatgtctg tcctgattat tatggagaat cctttctgct acacttaatc
     2101 ctctaggtcc ggcaccaatg atccctattt tcatttgcat gcctccattt cgataaattt
     2161 tttcaataaa acagcatggt tctgatgttc attttttgca ccataaacaa aagtaatgac
     2221 tggatgttct ttcataagtt gactgagttt aaaaatagct tgcttctttt cttcttcgaa
     2281 aatcaattct tgctggtatt tctttgcaaa tgaagcaaat cgttctggta catggtcaaa
     2341 agttttgcgt gcttcattac taggtgcaat ttctttcaac cacagatcta actgcgcttt
     2401 ttcttttgaa attcctcgtg gccagaatct atccacgagt attctatagc catcctcttt
     2461 ttcagcatca aggtaaacac gttttaattt gatcatagtt gtttccttct ttctaccttt
     2521 tcgcttagct tatatatgta gataatagca caaaataaaa aaaatgaacg gcacaactat
     2581 ccattgcgcc gttcatgtga tcttggtcaa aatctaatga ttgattcggt ttttaaagct
     2641 gtcatacaat catctgtttt atactttttt acttttgatt tttccagacc atccgtcata
     2701 tccaccctta agtaaataaa tctcttgata acctgcttta cgtaatttat ttgctgtacg
     2761 gatgctcaaa cttttctttt gatcgtaaat atagacaggc tggtcttttc gaatagatcc
     2821 taatgaatct ttcaaaacag tgtaagggat gtttcttgca cctaaaatat gcccagcatc
     2881 aaacacatct ttttcgcgaa catcgattac ttgtgctttt ctcattcctt ctcgaaattc
     2941 ttcttctgtc aaagtcgttg ctgaacgttt ggccattact cgtagataga cttcccaaag
     3001 gatgattgca agcacaatca atagcaaaat gatattaatt acccataaaa ccatgtaatg
     3061 cctccgtttt tttaagcaaa tttcaaggct aatgaggcag caccaatgac accagcttca
     3121 ttaccaagct ctgctaattt gatttttgtt gattctctta cttgcgggaa agtaaattct
     3181 ttaaaatatt tctctacacg gctacgtaaa aattctcctg ctgccgatac tcctccacct
     3241 aacacgatac ttgacggatt taatgtattc cccaagtttc cgcaagctaa acctaagtag
     3301 aagcatacgc gatcaacaac catcaatgca aacggatcgt cttcttgagc catttcaaag
     3361 atatctttac ttgttacttc ttctccgttg tctagcattg ctttcagttt tgaatcccct
     3421 gcgtattctt ctgccaagaa acgacctagg cgtaccacac ctgtagcgct agatacagtt
     3481 tctaaacatc cgcgtttacc gcaagtacat tcgaaccctt caggatccac agtgatatgt
     3541 ccgatttctc ctccagctcc gctcacgccg tgaatcaaat ttccgccagc aacgatacct
     3601 ccgccaacac ctgttcctag agtaacgaat acaacgtctg gatcgttttc tccagcacct
     3661 ttccatcgtt cacctaaagc agcaacattc gcgtcattat cgatagcgaa tttgattcct
     3721 gttcctgcct cgatcagttt tttcacaaat tgaacttctg tccagtttaa gttatacgca
     3781 ccaataaccg ttccagcttc gctgtccacg cttcctgggg tacccatgcc gattccgata
     3841 aagtcttcgg ctttcatatt atataaattc aaacgatgat tgattgattc aatgatttca
     3901 ggaacgatat gcttaccatc atccaaaata ttggtatcga tgctccattt ttgctgaatc
     3961 tctccttctg gtgttaaaat tgcaaatttt gctgttgttc cacctaaatc aatcccaata
     4021 atcttcttgt ccattacaaa tttcctttct ttcggctttc ttcaattcgg tgctctcttt
     4081 ttaatatgcg ccacgctgtc atgtatgtat catgatcaat caaacggcta tcatacagat
     4141 tacgcaattc gatagtcatt aattcaatgt catagatgcg tgcacccaca tagataaaga
     4201 tgccaaattt tttgaacagc tgctgtacat catataagct ctccatcttt aagcacttcc
     4261 ttcatattat acaagtaaac catatttttt taaacccagt aaaatacata ttacaaaaag
     4321 aaatacaaaa actagtgtag aaagaatcct ttggtgtatc gaatagcttg tctgttgttt
     4381 cggtagaccc aaaacattcc ctaacactaa tccgccaatt agcccaccga catgtcccca
     4441 aacatctaca gaggtatcca gaagaccaaa aacaaatgtc aacacaataa ataaaagaaa
     4501 atgtctgact agctgctgga tcgctgggtt atgtttaaaa tgaaacccta agataaacaa
     4561 cgcaccgaac aaaccgaata tcgaagtact tgcaccacca gacagaacat tcgcttcgtt
     4621 aaaagcaaag cttgctgtat ttcccattat cccgctgaac atataaatca agaaaaaacg
     4681 ccaatggcca tagattgcct cgatttgctg ccccataaaa tacagtatca gtgagttcat
     4741 tacgaaatgc atcagtccaa aatggatgaa gattggtgtc actaaacgcc accattcatt
     4801 gaagtgaaca agataaggac catacattcc ggcataataa gggatctcaa gtcgcggaaa
     4861 taattccatc atgaggaaga ccgctgtctg tatccctaaa aaagcatagg tccataacgg
     4921 tcgtcttaac catacacgca attttatttg ctgttgataa ttcatgaaaa cctcccattt
     4981 cattttagtc aataaacaat tgttggatct tctgatcaaa gctctccggt tgccattgtt
     5041 cattcaattg ttcacgaaaa acaaggctac atgttttttt atgaaaatta tttaaatacc
     5101 ggtcgtaata accaccgcca aaaccgattc gataaccggc tctattgtag ataatacctg
     5161 gtacaatgag gagatcgatc atctccggca aatagagctt ctctgattgt ggctcaagaa
     5221 caccaaaact cgaacgtaca agaacggact gttcatccat ttcataaaac gccatctgac
     5281 gtttcggcaa agttttaggt acaactattt tcttttgttc tttttctgct cgttggataa
     5341 ccggctgtgt atccagttca aattcattcg ataaagtcgt ccctatcact tttgctgact
     5401 tccagtactc gctggcgaat agctgtttat agattcgctg ttctttttcc tttttcttgc
     5461 cacttttggc cagatttttc agcttttcga ttcctttccg gcgtaatctt actttcaatt
     5521 ggtccaaaaa ctcacccttc cttctctcat aaaatggtat aggaaaaaaa gaaaaatagc
     5581 aatcgaactt tcaaataaat gcataaatag aataaaaaga agacctagaa atgattggcc
     5641 ttcctttttt aatgaatttc aactgttcag cgcgtgtttt tgtctgataa aaagtgcata
     5701 cagaaaatat ggcgcaccaa aaagggcaat caaaattcct gtaggtactt ccccacttag
     5761 tagaatcact ctagcaacca tatctgcact aagcagaaaa atggaaccga acaaaccgct
     5821 cagaacaaag gtccagttat tttctttacg tacgactagc ttcgcagcat gaggagctag
     5881 tagtcctaca aaagataaac tacctgtcac cgctacacta ccgcttgcca aagcaacagc
     5941 taacaacaaa aggatttttt gacttttagc cacgttaagg cctaatcctt gcgcgcgttc
     6001 ttgaccaaat gatagcaata caatcctctg tgcataaagt cctcctagcg gaattaagat
     6061 aaccgcccat ggaagcaatg cccaaacata aggccagctc gcaccccaga ttgatcccgc
     6121 taaccatgca ttcacaaatc cataattttc cttagataat tggatcgtgc atagcaatgt
     6181 taatgcagaa aggccggcat taacggcaat accggacaat aagattcgat tcatacccaa
     6241 aaagccattt cgatcatagg ccgcaagata gacacagcaa gcagctaaaa caccacctag
     6301 aaaggctaca actagcaata gaaacggcag cgaatagtgc gaaaagaaac ctaaataaag
     6361 catcactaaa aatcctgctc cggcattaat gcccaatata ctagaatctg ccaattcatt
     6421 tcttgttact ccttgcaaga tataaccaga aacgccaaga caagctcctg ctaataaact
     6481 gatcagtaat cgaggaactc gaaattctct aacaatcagc gccaaagaag atgattcttt
     6541 ccctatgaac gtggcaaaaa gctgatccag gtaaataaaa ggcgtaccaa tcattaaact
     6601 acctatagac aggatgacaa gtacaaaaag caagaaatag aacgtccaat atattttttt
     6661 caccgttgtt ccctccttac ttgtatcagt aaaaacggaa cgccaatcaa tgcaatgact
     6721 aatccaaaag gtgtttcaaa agggggattg atcatcctcg ctgtaaaatc cgccaacata
     6781 accaatccac cgccaactaa aaaagagaca ggtagaacat aacgatagtc cgttccaacc
     6841 attccgcgaa caactggcgg aaccaacagc ccaataaatg aaacaggtcc gactaacgaa
     6901 acggcaatgg ctgctaacac tgcaactaga cagaaaacaa tcaaccgcgt tcgtttagga
     6961 tgacttccaa gagctaatgc atgtgtttca ctcaaatgaa tcaaagatat tgcttttcct
     7021 aatgaaaaag aaaccaaaac agcaagtata tataaaggaa acaatactgc taattgtttc
     7081 catgtgatat tggcagctcc gcctacaaac caaaaagcaa gatcctgctg cagattaaag
     7141 aattgggtaa gtgcttggct gatagcagta aaaaaagaac taatagctac gccagctaaa
     7201 atcagctgta aaggattcat gcccattttc gaaaactgca aaaaaccaaa tacgagcaaa
     7261 gttacagcaa aagaacctgc caatgacatt agaaacacag aaaaatcaga agctttaggc
     7321 caaaaagcaa aactaagggc cattgccaac gaagcacccg cgttgattcc tagtaagcct
     7381 gaatctgcta aaggatttct tgtcactcct tgcatcaatg caccgctcaa tgcaaaacaa
     7441 ctccctacaa gaaatgctcc cgccacacga ggcaaacgaa tatttcgtat gatttggtga
     7501 ctttgttttg atctatcaaa atcaaacaac gcctgccaaa aatctgcttt tcccacagtc
     7561 gctgcgccat ttgaaagagc aacaaaaaaa ataatcaaaa gaacaaaaca gaccaaaagc
     7621 aggagtctat ttttcttcat agtcctcata attgatcctc ctgctctttt ggcaacaaat
     7681 cataggttag aaaaactggt ttatttgttc ttggaagcgt gacaatcgtt gcatcaatct
     7741 caaatatttt tttcatacac tcttttgtga tcacttcttc aggtgtacct tctgtgatca
     7801 acttaccgtt tttcattgct aataaataat ctgaaaaacg tgaagcatga ttaatgtcat
     7861 gaatcgacat caagatcgtt gtttgtttca tttgattgat ttcagtcaat aactgtagga
     7921 tctctaattg gtgtgccgga tctaaaaatg ttgtcggttc atccaagatc aagatatctg
     7981 tttcctgagc aagcgccata gcgatccaga cacgctgggt ttgtccacca gacagcgttg
     8041 cgatatcccg catacgaaat ccagctagac ctgtagcatc caacgcccac tcgattgctt
     8101 catgatcttg cgtgtccatg ccactgaaca gtttttgata agggtatcgt ccgtaagata
     8161 ctacttcttc tactgtcata cctgtagggt gttcactagt ttgtgctaat aatgcaattt
     8221 tttttgcaag ttcttttgaa ctgtatgtag agatatcaga gttattgaca atgatctttc
     8281 ctgatttcac aggtagtata cgggtaaaac ttttcaataa agtagatttt ccacttccat
     8341 ttggcccaat caaactagtg attttccctt caggaatcgt aacagacatg ttttcaatga
     8401 tttttttgtt ttcgtagccg gctgcaatgt cagctgctct taatctcgtc atttcaccca
     8461 tcctttcgct gaaaatcatt ctcaattatg acgctgtttc aacatttcgt caagcgctac
     8521 tttcatatct atagccaaat gaagcggttg acagcgttcg aaatatcagg aataataacc
     8581 aatgagaaag attctcaatt atgttaggag gaaagaaatg aaattaaaaa caaaagtatt
     8641 cacattatct agcatattgc tgctggctgg agtactgagc gcttgctccc cctcttcaga
     8701 taaagaaaca gctacaactg ccaattcgac gacttcatcc aatagtactc atacagtcac
     8761 agatacactt ggacataaag tagagattcc aaacgcgccc aaacggatca tcggcagtta
     8821 cttagaagac tatctgattg cactagggga aaaacctatc gcccagtgga ctgttggcag
     8881 tggttccatt caagattatc tgcaagatga tttaaaggat gtcccaacga tttcttatga
     8941 ccttccttat gagaaagtgt taagctttga gcctgatttg ctgttgattt cttcttccgc
     9001 tactgtcgaa ggcgggaaat acgaacaata tagcaaaatc gcgccaactt atgttgtaaa
     9061 aaacggaaca gatgtcacat gggaagaaca actaaaagat gtcggcaaag ctttggcaaa
     9121 agaaaaagag gcagaaaaga tcatttccga ttatcaaaaa tccatcaaga aaactcgaga
     9181 agaactagca gataaaatcg agaacaagac agctgcagtg ttgtgggtga ccaacaactc
     9241 agcctttatg gtcagcaaaa atcggtcaag tggaagaatc gtatacgacg atctggaatt
     9301 tggtgttcct gatctagtgg aagaggtctc caaaaaagcc acttctgact ggtctgctgt
     9361 ttcctctgaa aagctagctg aattagatgc tgactacatc atccttgtga acagtgacaa
     9421 agatgctgcc atgttcaatg aaccaagctg gaagaatctt aaagcagtga cagacaatca
     9481 tgtactgcaa ttcggacctg aatctagttg gctatataac ggacctattg ccagtcagga
     9541 aatggtcaat gatatcaaag aaaaactaaa ttgattttaa ctaaaaaaag catcttcacg
     9601 attagtcgtg atatgctttt tttcttatta acaaactaga cgcatagatt ttattcttct
     9661 tttgaagcaa ccctattttt tactctttcc catttttgaa aacgatgggc atatagattt
     9721 tcttgatccg ttatcccctc atactcctct ataagggcga agtctgaaaa atcaatattc
     9781 ccaatatacg tatcgccttc aaacgtgcta tcgatcaatg ttcgccagat ccgaccagtg
     9841 taaggaagaa gttcttgaaa aatccgacta ccacccgaaa catagatatc ttctgagatc
     9901 gtttgactat acgacaaaag ttcatcgatc gaatgcatta cttccgcatt ttctttcgtg
     9961 cgaaaatttg gctgcgttgt caacacgatg atatggcgat aaggaagtga aagatccccc
    10021 attccttcat aggtctttct tcccatcact aaaattcgat ccattgtatg ttcgcgaaag
    10081 aaacgcatat catttggcaa tcgccatggt aacaaaccat ttttgccgat cagaccattt
    10141 ttatcttgtg cccacatgga aataaacata ttatccgctc cttatgattt tagatgtttt
    10201 gagataaaaa aagtctcaat tctttgacca gcaaggaatt gagaacttta aaaacattat
    10261 tttgtttcac ggtgcaaagt aactttgcgt tcgcgtgggc aatatttttg tttttccaaa
    10321 cgatcaggat tgttacgttt gtttttgctt gttagatagt tacgttcttt acaagaagta
    10381 cactctaaag tgatgtttac gcgcatgttt ttccctccca tttttacaag aatcctctaa
    10441 gatgactctc agacttgatt atcttaccat gattttttct agattgctag tgtttttcac
    10501 gattttgtta agtaaaacta cttgacgata aatacaacgg ttttacttct caaacaaaat
    10561 ctttttcttt ttgtataaat acccttacac ttgtttatat agattatgtt ttttaataaa
    10621 gggggatact aatatgccat ggaacatgaa ggactttcct gcatcaatga aaaacttaga
    10681 taaattgaca agaaaaaaag caatcgacat cgcaaatgca ttattagatg aagggtaccc
    10741 tgataaccgt gcgatcccaa ttgccattga tcaagcaaaa aaatggtatg aagatgctag
    10801 tgaatcagaa aaacgaactt tcgaaaaaga aaaaaatcca agcaagacgg atgaacacga
    10861 tacgaatcct cgtgctggaa aactgcttga ttcagatgtg atcgtagaat atgaggaaga
    10921 acaatggatc gtgaaatcta aaggggcaaa aaaggcaagc aaccattttg atacaaaaaa
    10981 agaagcaatc gaaaaaggaa agcaagtagc acaaaacaaa gaatcaactt tgttcattta
    11041 taaaaaagat gggacaaaag aaaaagaaat cgattattga acatatgtaa aggggttgtg
    11101 acgaaaatcg tgtcacaacc cccttatgtt cgaaacaatg gtgttcctct gccaactccg
    11161 aaccataagt cataatatct ataaaacggg aagagctgtt ttttgaaatt gtttcttatc
    11221 actcagtgtt aaacggtagg agaaaacctc ttttttattg atatttgatt ttcatcaaac
    11281 agtcacatag tcgattgctt ctgcttgcgg aatcgtttca tctacaaagt agactttata
    11341 ccatgtcaag aaactgaaga ttgtccagat ttttctttgc tcttctttac gtccttcgtg
    11401 atggtcatct agcaacttca tgattttttc ttgatcaaaa aattcttttg caaaatctgc
    11461 ttcgaacaat gctttgactt gttcgtagcc gtgttcttct ttcaaccaag ctttgattgg
    11521 cacagggaat cctaatttca cacgattcga ccatttttcc ggaagatgtt tgttcgcagc
    11581 ttgtctgaaa atatcttttg tgttgttttg attcaataaa aacttacttg gtatttgttg
    11641 agccaatttc atgacttcga tatccaataa aggtactcgg acttccaaag aacttgccat
    11701 agaaagttta tctgccttta acaagatatc ttttggcatc cattgatgaa gatcgacata
    11761 ttgcatctta ttgacttcat ctttgattcc ttctgttttc tcgtaatgaa cagtcatgat
    11821 ttcatccaca gatggtgctg tttgatattt tggtgttaac acttctgatg cttctgattc
    11881 ttcgaacact cgagcatgac cgataaaata ttcttttgca ggagcaagtg attcatataa
    11941 atggatccgt ccatggaaat ttttcatttt tccgatttta cgtcctaaat tatagcgtgt
    12001 tccttttggt aattttttca atccttgagc aatcacacgg atgaattttg aattcgtatg
    12061 gaatccatac gtttggtatc ccgcaaacag ttcatccgcc ccttctccag attggactac
    12121 ccgcacactt tgtgaagcaa gatttgctaa aaagtacaaa ggtacgcaag aaggatttga
    12181 atcaggttca tctaagtgat actggatcaa agggaatgct ttgaacgaca tgtctccgtc
    12241 gatcactgct gctgtattat ctaagtccaa tagttcggtt agttttctgg cttcaatcgc
    12301 ttcattatag gttttatcat caaacccgat tgagaaagaa tgatctggtt tcaatacaga
    12361 ggttacataa ctggaatcaa ccccgctgga taagaaagaa ccaacttcta catcactgac
    12421 cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt ttttcgatcc attcttgtcg
    12481 ggaatgcgtt tcttttgttt caaaatctgc atcccaatat tgctgaatgt ctatttttcc
    12541 atctttatac gtataataat ggccttcagg caaacgatag acatctttga agaatgtttc
    12601 gccgtttaat ggagaatatt ggaaagtcat ataaggtttc aacgcttctt tgttcaattc
    12661 tttgttaaaa tcaggatgag gcagaaaact tttgatttct gaaccaaaca taaatgttcc
    12721 gttcatttct gcataataat atggtttgat cccaaagtgg tctcttgcac cgaataactc
    12781 attcttttca ttatcccaaa tagcaaaagc aaacattccg cggatctttt gcaataattc
    12841 tgttccccat tcttcgtaac catgcaaaag gacttctgta tctgcatgtg tttgaaaaac
    12901 gtggccagct gcaatcaatt cttctctcaa aggctggtaa ttatagattt cgccattaaa
    12961 aataatgatc tttgtccgat cttcattgta aataggctga gtaccgccct ctaagtcaat
    13021 aatacttaat cttctgaatc ctaaagcaac atgtttatcg atatattctc ctgaactatt
    13081 aggtccacgg tgaacgattc gatccatcat tgtattaata attgtttttt tgttgtcctt
    13141 gtcgttaaca aaacctacaa taccgcacat ttaactttca catcctaata taaaattact
    13201 gtttatttca ttcatatgca tcttatcata gaataaatag cggacgaata ttttctctct
    13261 tacaatttcg tcacgtgaaa acaaaagatg aaagtttctg tttttacaga aaataaagtc
    13321 ccaatatcta atttaccatg ttttcttttt tgaggctacc ttattctctt cgtgttaaaa
    13381 aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa aagacacgac ctaaagatcg
    13441 tgtctaatta tgactgtatc agaaaatatg ataccaaacg gatctattca ttaattaatt
    13501 gacatagttt atctgttgct cttttatgac tggataactg accaattcat gacgatccaa
    13561 gtcttcttta ttcatattta cagcagtaat ttgactgtct tcatacgtac gataataata
    13621 aatcccttta tcaacgttac agcaagaaga ataaatcgta tattcgtatt ttctttctcc
    13681 agtatcgcat actccttttt gttgctcgac tgagcctagt atatgaaaaa attgactgat
    13741 actttcagat tctgaatcac ctgatgcagc attcatttta gtaaatgtgg cttttacaaa
    13801 tcgagaaaca gaagagagat ctccaggtaa gcccaatcct cccatcccac gactataaac
    13861 attcaagctg atctggtttg aaaaatgatt ttctggtgtt ccatttgata gcgagcgata
    13921 attgttcaga ttaaataatt gataatcaaa tggcggatta tttgtcaaca caccgacagg
    13981 attatcatat aaatgaagtc catctttcat actttcaatt actatcgatt tttctttatc
    14041 agctaataac cagtgtaaag gtgacaaggg tagtttttca ctaaaatcga tattgactag
    14101 attgatagta gccaataatt ctttagcttc gtctatggtt gagcattgcc ctagtatcca
    14161 tggaataaac tcaaatggtg caacgttatc ttttccttct tgtaattctt tataatcagc
    14221 attgccagga aaattcagtc ctgccatact caaccctttt tcatttgtag cctcataata
    14281 gagtggatag ttggataccc cagctgctat tcctatcatt gcataatgtt tatctaaatt
    14341 ttttaccttt ctaaaatcga aacgataatt tcttggagta atagtgacca cttcattata
    14401 agacatctca taatcaaaat tccttccaaa ataatggtct ttcgttgcat aagtgattgc
    14461 ggtacacatc gtttcccctc ctaaaacaaa tcgtttttac acttttatct tacatctagt
    14521 atttttttta tcaaaaaaag gccaaagaag attgatttac tagcatttat aaaaaatagt
    14581 actcaggggg aatcaaaaaa tattgtaggt taaaagtaca acaataaggg gctgtgacaa
    14641 aagtcttgtc acagcccctt attccgaaca aacggtgttc aaaagcttgc atctgcggta
    14701 ataagcccaa tcaaacgaaa aaataggaaa agctattttt gtttggccgt tcttattact
    14761 tgatgcagga cagcttcttc acaacctcta atctttgata taaggaaggt acaggatttg
    14821 aacctgcgcg ccggtattag ccggttcgcc ggatttcgag tccggtgcat taccactctg
    14881 ccaaccttcc gtaagtacta tttttaaact tttttcacga aatgtcaagc ctatttctaa
    14941 tagataaata acagaaatcg ctttttttag caaaaaagtc cattgacagt ttgcagccaa
    15001 cggacttacc gatcaaattc atactgatag aacactatct gattatttca ggattatcta
    15061 atgctaaaat cgagtcaatt gtccgtttga tcagtgcaat gatttgttct tctgttggat
    15121 gcatgatgtt tgatgacatc aatgacgcta agccagtcac tgctacccat gtaccagtat
    15181 gcagagcatc gacatattca tccgataatt gattgtatct ttcatcttct ttaactattt
    15241 cacggaaata attgtatgaa aaatcatgca ttttctttcc gtccccatgt tcatcaatgt
    15301 acaatgccat gtaaagacta tgatgctttt ttgcaaagta gatatagttc aaaccgagat
    15361 caatgatttt atcaccagta cgttcaatag ggaaaacatc ttcttttagt tgttcaaaga
    15421 tagaatccag caacgtattt tttaaatctt ccatgttttt gaattccaga taaatcggct
    15481 gtgtcgaaat ccccatcttt ttagcaatat ttctcgcagt aaatccttta aacccttcct
    15541 ttgcaacaac atcataagca gcttttagaa tttgatcctt ggtatagact tttcttctca
    15601 ttaaaactcc cccacttctt tactgcagta tatgttgttt tattgtaccc tatttttagc
    15661 attttttgca aacattttac tcaaaacgtt atattttaaa ttttttttca gtcttcttta
    15721 actttttttt gc
//
LOCUS       DAYYBH010000057        15255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000057 DAYYBH010000000
VERSION     DAYYBH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.059"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACHAUG_002061"
     CDS             2..1021
                     /locus_tag="ACHAUG_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8481143.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACHAUG_002062"
     CDS             1018..1785
                     /locus_tag="ACHAUG_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8481144.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACHAUG_002063"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACHAUG_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8481145.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACHAUG_002064"
     CDS             3750..4070
                     /locus_tag="ACHAUG_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8481146.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACHAUG_002065"
     CDS             4070..4420
                     /locus_tag="ACHAUG_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8481147.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACHAUG_002066"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACHAUG_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8481148.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACHAUG_002067"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACHAUG_002067"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8481149.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACHAUG_002068"
     CDS             6414..7439
                     /locus_tag="ACHAUG_002068"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8481150.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACHAUG_002069"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACHAUG_002069"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8481151.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACHAUG_002070"
     CDS             8517..8957
                     /locus_tag="ACHAUG_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8481152.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACHAUG_002071"
     CDS             8960..9562
                     /locus_tag="ACHAUG_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8481153.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACHAUG_002072"
     CDS             complement(9625..10728)
                     /locus_tag="ACHAUG_002072"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8481154.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACHAUG_002073"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACHAUG_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8481155.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACHAUG_002074"
     CDS             12294..12689
                     /locus_tag="ACHAUG_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481156.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACHAUG_002075"
     CDS             12689..12940
                     /locus_tag="ACHAUG_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481157.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACHAUG_002076"
     CDS             12983..13378
                     /locus_tag="ACHAUG_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481158.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACHAUG_002077"
     CDS             13375..14631
                     /locus_tag="ACHAUG_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFM8481159.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACHAUG_002078"
     CDS             14643..14966
                     /locus_tag="ACHAUG_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481160.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACHAUG_002079"
     CDS             14959..15225
                     /locus_tag="ACHAUG_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481161.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAYYBH010000058        15230 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000058 DAYYBH010000000
VERSION     DAYYBH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.060"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACHAUG_002080"
     CDS             278..787
                     /locus_tag="ACHAUG_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8481162.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACHAUG_002081"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACHAUG_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8481163.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACHAUG_002082"
     CDS             2322..3302
                     /locus_tag="ACHAUG_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8481164.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACHAUG_002083"
     CDS             3424..4842
                     /locus_tag="ACHAUG_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8481165.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACHAUG_002084"
     CDS             4913..6382
                     /locus_tag="ACHAUG_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8481166.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACHAUG_002085"
     CDS             6392..6859
                     /locus_tag="ACHAUG_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8481167.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACHAUG_002086"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACHAUG_002086"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8481168.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="ACHAUG_002087"
     CDS             7849..10383
                     /locus_tag="ACHAUG_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8481169.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="ACHAUG_002088"
     CDS             10539..11312
                     /locus_tag="ACHAUG_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8481170.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="ACHAUG_002089"
     CDS             11305..12096
                     /locus_tag="ACHAUG_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8481171.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="ACHAUG_002090"
     CDS             12106..12597
                     /locus_tag="ACHAUG_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8481172.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="ACHAUG_002091"
     CDS             12615..13025
                     /locus_tag="ACHAUG_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8481173.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="ACHAUG_002092"
     CDS             13029..14429
                     /locus_tag="ACHAUG_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFM8481174.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="ACHAUG_002093"
     CDS             14452..14826
                     /locus_tag="ACHAUG_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8481175.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2137 c         3044 g         4762 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta
//
LOCUS       DAYYBH010000059        15104 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000059 DAYYBH010000000
VERSION     DAYYBH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.061"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..666
                     /locus_tag="ACHAUG_002094"
     CDS             <1..666
                     /locus_tag="ACHAUG_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8481176.1"
                     /translation="ILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISY
                     FVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASV
                     MGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPR
                     NSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDS
                     ALF"
     gene            678..1598
                     /locus_tag="ACHAUG_002095"
     CDS             678..1598
                     /locus_tag="ACHAUG_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8481177.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1639..3096
                     /locus_tag="ACHAUG_002096"
     CDS             1639..3096
                     /locus_tag="ACHAUG_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8481178.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3441..4055
                     /locus_tag="ACHAUG_002097"
     CDS             3441..4055
                     /locus_tag="ACHAUG_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8481179.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4048..5772
                     /locus_tag="ACHAUG_002098"
     CDS             4048..5772
                     /locus_tag="ACHAUG_002098"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8481180.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5769..7220
                     /locus_tag="ACHAUG_002099"
     CDS             5769..7220
                     /locus_tag="ACHAUG_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8481181.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7693..9846)
                     /locus_tag="ACHAUG_002100"
     CDS             complement(7693..9846)
                     /locus_tag="ACHAUG_002100"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8481182.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9848..10894)
                     /locus_tag="ACHAUG_002101"
     CDS             complement(9848..10894)
                     /locus_tag="ACHAUG_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8481183.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11044..12333
                     /locus_tag="ACHAUG_002102"
     CDS             11044..12333
                     /locus_tag="ACHAUG_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8481184.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12346..15048
                     /locus_tag="ACHAUG_002103"
     CDS             12346..15048
                     /locus_tag="ACHAUG_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8481185.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         5089 a         2638 c         3047 g         4330 t
ORIGIN      
        1 atccttttga atctgctgat ctcagtcgta tttgcaatca ttatgagcga gttgagaaat
       61 aaacgactcg ttaaagttta tcaaacgatg tccttactac cttattttct atcttgggtc
      121 attatcagtt actttgttta cgctttttta agtcctgaca aaggaatatt caatcagtgg
      181 atcactggac atggaggaga agctgtcaat tggtataatg aaccgaaata ttggccgttt
      241 atcttggtgt tcattggtac atggaaaggg attggttata acagcattat ttactttgca
      301 tctgttatgg gaatcgatcc gacttattat gaagctgcta tggttgatgg agctagcaaa
      361 tggcagcaga tcaaacatgt gacgatacca caacttattc cactaatgac gattttaacg
      421 attttagccg taggaaacat tttccgagct gattttggcc tgttttataa cattcctagg
      481 aattccggtg cattatatga tgtgactcaa gttttggata cgtatatcta caatggcctg
      541 acatccacag gagattttgg tatgacggct gctgcaggac tttaccaatc agttgtcggc
      601 tttgtcttgt tgatgatcac aaatacgatc gcccgacgtt ttgatagcga ttctgcatta
      661 ttctaaggag gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc cgctctttca
      721 ataagacggc taattttttc tctaatataa tgattgcact ctttgctatt tcctgtattt
      781 tgccatttat atttgttatc gccatctctt ttaccgatga gtccatattg tcgactcacg
      841 ggtacagttt ttggccagaa aagttcagta tatttggtta tacgtacttg ttcgaccaga
      901 tgcaggataa aattttccaa gcgcttttcg tcactgtact tgttactgtt ttaggtacac
      961 tgatcaatag cacggctact tctttatatg cttatgcgat ttcacggtca aactttccat
     1021 tccgtcgttt ttttaccgtt ttttgtttga ttacgatgtt gttctcacca gggatggtgg
     1081 ccaattatct tgttatgaca aatcttcttc agttaaaaga tacgatttgg gcattgatct
     1141 tgccaatggc tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt aaacgttcgg
     1201 tctctgattc gattattgaa tcggcacgga tcgatggagc tagcgaactg cgaatatttg
     1261 ttcagatcgt tttaccgtta gctgttccag gtattgcgac aatcagttta tttgccgcat
     1321 tgggttactg gaatgactgg ttcaatgctt tactatacat ccaagatgat aagctagttc
     1381 cactacagta tcttttgatg aaaatccaaa gtaatattca atatctgaca caaaattcag
     1441 gagcaggaag ccaattgaca ggcggattag cctcagttcc aggagaatct gccagaatgg
     1501 cgatcgtagt catctcgacg ttgcctattg cgatcagcta tccatttttc cagaagcatt
     1561 ttgtcaaagg tctaacaatc ggcggagtaa aagaataaag aaacaaaaat ataaatcata
     1621 aaattaggag gaagaaaaat gaagttatgg aagaaactag cctttacagg tgtttcagcg
     1681 ttgttgttgg gaacattagc agcatgcgga ggttctggtt ctaaagacca ggcagaagct
     1741 agcaattcgg acactttgca gatgtatcaa atcggagata aaccagataa tttcgatcaa
     1801 ttgatggaag tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt caatatcaat
     1861 tacattggct ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc cggggaaaat
     1921 tatgatatcg cctttgcgaa caactatgtt tccaatgccc aaaaaggagc ctttgctgat
     1981 ttgactgaat tagcgcctaa gtacgctgaa aaagcttata atgatttaga tgaagcatat
     2041 attaaaggaa atctggtcaa tggaaaatta tatgctttcc cagtcaatgg aaatgtcttt
     2101 gctcaacaag tacttacttt taacaaagct ttactagata aatacgatct ttcaattgat
     2161 ggaatccaat cttacgctga tgcagaaaaa gtcttgaagg aattccatga aaaagaacca
     2221 aacacggcag cttttgccat tggacaaggc ttcaaagtcc aagggaactt tgattatcct
     2281 ttagggaata cgttaccatt tgctattgat ctggatggag atgattcgaa aatcatcaac
     2341 caatacgata atgaaacgta tatcggactg ttacgtacaa tgcatagctg gtacaaacaa
     2401 ggactcattc caagtgacgc tgcaacgagc aataaggatt atccactaga aggaaatact
     2461 tggctaatgc gtggagaaac acaaggaccg tatgattatg gcgatacaat tttgacaaat
     2521 gcagcaaaac aagaacttgt ctcaaaagca attactgttc cactaaaatc tacagctcaa
     2581 gcacaaatgg caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa atcagtagaa
     2641 cttctagggc tgcttaacag tgatccagaa ttattgaatg ggcttgtttg ggggatcgag
     2701 ggagaagcat gggaaaaagt cgatggttca gatgataaaa tcaaattgct agatggttat
     2761 cagccaaata cacatatgtc tgcttggaat acaggaaaca acaagattct atatacacaa
     2821 gagtctatta cggatgacat gattgccaaa cgtgatcaat cgattgctga tgcagaaact
     2881 tcaccgattt taggattcag cttcaacact gacagtgtca aaacagaatt aagtaatatt
     2941 tctaatgtca tgaatcaata tttagatgga ttgaatacag ggactgttga tccagatgaa
     3001 acattaccga aattgaaaga cgctttggac aaggctggct atgataaggt attgaaagaa
     3061 atgcaaaaac aatatgatga gttccgacaa gaataataat gaataaagac aaaggggttg
     3121 tgacaaaaga cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa gccagtttct
     3181 cggtattaag ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa tttttcttac
     3241 tactcgaaac taagcggcta tttcacaaac tcttggtaaa cggtgttcaa tcagctaacc
     3301 ctcggtattc gctcacaaaa cgacaaaata tgaggaacta tttttccgtt tgtttctcta
     3361 ataatcaggt caaggtgctg attgcccaac ctttttttgt ataatgagaa agtataaact
     3421 taatgtttgg aggagaagat atgttaggga aggttttgga agagttattt atccggatct
     3481 gggtcattat aaaactaacc ttgtatttct ggatatatac atttgccggc gggatcatct
     3541 ttggattagg tgcggcatgg aaaacagtca atgaactatt ttatttgtac ggattcgagt
     3601 ataaagaaat cacaataaaa agaggctgga atatatataa aagaaatttt ttaagaggca
     3661 atctgctatt tagtttattt ttatctggta ctgctttatt gagttacaat ttatatttat
     3721 ctgttcaaat caaagggctc atttttttag ctattgattt tattttactt tttgccttgc
     3781 tctgcctatt tgctacttat caatacagta tgattttaga tagtgaatat acgatctctg
     3841 ttcctaatct gctaaagcta agttttattt cggttttttc cagcttttcg tcttttctaa
     3901 aaataattat tggtagcggt ataattttag gcgtgacttg gcaattcaaa ggattgatcc
     3961 tgtttggtgt cattggtctt ttgacggttt ggaatggtac gatgacaaca cattggcgtg
     4021 aagaattaga taaacagttg gaaagttatg aataagtttc ggattctatt ttcaaaagga
     4081 accttactaa atcgtctgtt gcggaaatac gcggttatca tgatcggtgt aacgatgact
     4141 gctaccgtta tttttagtat ccatacttgg gaacaaaatc aaaaacaggc agaaaacatg
     4201 acaagtgatg ccgttcaaag cacaagcaga atgctgaatg ataaaacaac cttgagtagg
     4261 atcatcaaaa accagcttgt agggaattcc gaaaagatcg aaaatgtgac gacctactta
     4321 acaaaaccca tagatcagta tctgatgtat gtatacgaac agcaaaacag taccgatgaa
     4381 ttagtttctt tcccaaatca aatcaaagat ctctacgcaa attatgaaga actaagtgcg
     4441 atatatatcg tgttgaatca actaccggag tattatgaat cttctagaga aaacaaaggt
     4501 ggggaaataa aaaaggggac accaaaatta ctagacgcct tttatatcaa gttgcctatc
     4561 acacaaggag gagctgagtc aggaagtatt tttattggtt ttcaaaagaa agaactagat
     4621 atgattttag aaaatctaac gtcattcaat ggactctctt tatacatgat ttctggaacg
     4681 acaaatcgac tatacacatt ccatgataat aaaatagcta aagaaacgtt tgaaagacaa
     4741 gaaatgatca tcacagagag tctgaagaaa aattcacaat tgccgattga cgatctggaa
     4801 aaaaacaatt ttgttcaaca ccaagaactg tctgatgatt atcgtatatt agctgtacta
     4861 gataaaaaaa gtgtaaaaat cgaaacggga aaaaatcttg ccccgctgat cattggtatc
     4921 attattttag acaatatact gcttatcttt ctttataaaa cattcaaacg atattcacag
     4981 caagtatcta tcgtcatgca ggccatggat caaacagcag cagggaattt agagacgcgg
     5041 atcgacataa caaacacgga atatgaactg aaggaacttt caatcggtat caacgaaatg
     5101 ttggatagca tcaatcagtt tgtagaagac atctacaaat tagaaatcaa acagcaagac
     5161 gcacatatgc gggcgcttca agcacagatc aatccacact ttttatataa tacattagaa
     5221 tatatccgaa tgtatgcaat cagcgaagga agtgaagaat tagcagatgt tgtgtatgcg
     5281 ttttccgctt tgcttcgaaa caatacgaat caagagaaaa cgatcacttt aaaagaagaa
     5341 ctggactttt gcgaaaaata tgtctatttg tatcaaatgc gttatcctaa tcgagtagcc
     5401 tatcatttta tgatcgatcc tgatttggaa aagatcgaag tacctaaatt tgtgatccag
     5461 ccattagttg aaaattattt taaacacggt attgatttta ctcgattcga taatgcgcta
     5521 agtgtcaaag tcttgcagga aggcaaacgg gtaaggatca tcatcaaaga taatggaaaa
     5581 gggatgactg aaaaacggct aaaacagatt gaagaaaaac tgtctcatcc taaagtggaa
     5641 cttcacggtt ctatcggtct gcaaaacgtg aatgaacgtt tacgtgccag cttcggcagc
     5701 agttattata tgtcgcttga gaataatgag acaggtggac tgaccgtttc tattacattc
     5761 aaggagggat gagcgtgtat caagtgttat tggtagatga tgaatacatg attttgaatg
     5821 ggttaaaaaa gataattgat tggcaaagtt taggctttca aatagtagca acagcagaga
     5881 atgccaaaga aggactagct gtacttgaac agcgacagat tgatttagta gtaacggatg
     5941 tgacaatgcc agaaataaat ggactggaat ttattgaagc ggctcaaaaa gaacgtcata
     6001 attttgaatt tatgatttta tcaggatacc aagaattcga ttatttgaaa ggaggtatgc
     6061 aattaggcgc cgtgaattat ttgatgaaac ccgtgaataa gttcgaattg gttgaaagcc
     6121 tcaagaaaat caaaacacgg ttagatcagc aaaatgaaca aaaaaatcaa caagaaatct
     6181 atcaagaact gttattcagt caatggttga atgaagaatt ggatgaaata agtgaagaag
     6241 aaataatagg acaaataagt gagaagcagc ataggatagt attgattcaa ctggcaagaa
     6301 ctcatgatac tcttatcaat agatggttgc aagaacggca acaaatcttt tactatcaac
     6361 gaaattacgg agaccgtgta ttgatcacgt tgttgttgga ggcagatgaa gttgatggct
     6421 tctgttgttt tgttcaagaa aactttcctg atcaggaatg gctgatcagt atcggggaag
     6481 agacagattc tttggacaaa atccctgaaa cattccaaca agcgaaagac aatctacagt
     6541 tgcatcaatt ttatggagat aaagacgagc atgtttttta cgcagtacaa gcagaaacaa
     6601 aagatcaaac catcgatttt tcaagtttca gacgcgtgct gcaaaataaa caattagaag
     6661 tcgctcaaaa aatgatcact gatttttttg aacagtttca gttagcagcc atgatgccgg
     6721 aagatatacg atattccagt tttttactgt ttatggagat tcaacgggaa ttgattgatc
     6781 tggaagatga ggaatattta caaggaatcg aaaaaatcca tcaggcaaaa acggtgcaag
     6841 aacttcagca gctacttctg tcattcatca aaaagcatca gagacagaaa aagtacagtg
     6901 acaatgtgga aaaagtaatc gtaatcctgc accagcatta tcaagaacca cttacactca
     6961 aagaagtgtc agaaaacctg catttaaatg tcatgtatct aggacagctg ttcaaaaaag
     7021 aaacgaaaaa aagtttttca gcttatttga accatttacg tatggaaaaa gcgaaacaac
     7081 tgcttttgca cagtaatcaa aacatcaatg agatagcaag tgagattggc tacaacaaca
     7141 ccacgtattt ctctaaatta ttcaagaaaa tcgtcggcag atcccctaaa gagtaccgag
     7201 aaaacagtgg agaatcataa aagaaaaagc caatgtaatg aaataactta taaaaaattg
     7261 tctagaaatc taattttggg ctgttcttat tacttgaggc aaggcagctt tttcatgacc
     7321 tctcgataaa cggagttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat
     7381 gtggaaagcc attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacga
     7441 cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaaa aaatggcaaa
     7501 atatgaggaa ctattttgcc atttgtctct ctaatactca ggtcagaacg ctgatttcac
     7561 aacctctcaa taaacggtgt tcaaaagctt gcatctgcgg taataagacc aaccaaacga
     7621 aaaatatgag gatctatttt tgcttggctg ttcttattac ttgatgcagg acagcttttt
     7681 cacaacctct tgttaattat agtttgataa ggaaaaaggc aaatcttttg cttgatattt
     7741 cttagggtga gggatgatcc ctaattggta agagatcgtg ccgcctttca gcaaatcttg
     7801 atgggtaaaa tataatttcg tatattcttt gtccatccgt ttgattttat ggataaattg
     7861 ttgctgcggc cgattaggcg ttgcttcaat cgtcaattcc tttccatttg acaaaagcaa
     7921 aacagccttg tcaattaatg gcataccgat aacatattca ccggttccag gcgtgacagg
     7981 ataaaatccc agactattga agatatacca agcggccata cttccattgt cctcatctcc
     8041 agggtaaccc attggagaat cgtcgaaggt ctgagtcatc aactgtttaa gcagtggctg
     8101 tgccatttca ggttgtccta tataactgaa caagtaagga tagtgaaaac ttggctggtt
     8161 tgaaatagct aattgtccaa attctatcgc ggccatctca ctcatttcgt ggatctcaaa
     8221 accatatcct cctgggtcaa acgttggggg ctgattgcac agctcaatga gtttttcttt
     8281 atatctctca ctgccaccat attttccgat cagtcctgaa aaatcgtgat aaacagcaaa
     8341 gctgctttgc caagcacttc cttctgcata atcttgtccc caacgattag ctgaaaatgg
     8401 ttctctgaac ttgccatctt tatcttttga acgcataaat cctgactcat agtcaaagat
     8461 attttgatag ttcaatgatt gctgctgata tttcttcgtc atttcctctt cttctagtac
     8521 cttggcaact tggctaatac aaaaatcgct ataagcatag tctaatgtgt gattgactga
     8581 ttcgtggtag tgattaggaa catagccata tgtcaagtaa tcttctgttc cttggcgtcc
     8641 ataatttgga tgtttgcttt gaaccgtagc acctttttcc attgccgtta gaaattcagg
     8701 catcaagtct cgtcggatat cttttacagc agcatctgca atgaccgcat cgatcaatgt
     8761 cccaggcata agtccacgtt catcaggaga cagccatttt ggcaaatagc cactttctct
     8821 atagctgttc aaaaaacctt ccagcatttc ttcgtatttt tctacagcaa tcaaagaata
     8881 aagaggataa accgttttat acgtatccca aaacccatta tttgtataga gaacaccagg
     8941 ttttatagat cgactagtcg tatcatagtg gattttttgc ccactaggat caatttcata
     9001 aaatgtttgt ggaaataaaa acacccgata taaattatga taaaacgtcc gcaattgttg
     9061 ttgatcatgg tgtgtgagct gtatacgatc aaaataggac tgccattgtt cttgactgtg
     9121 agcaaggtaa gtctctttgc tttggttttg ttctagttcc aggttataag ctgcttgttc
     9181 cgagctgata aaagatgtcc caaatcggat agtttgtttt tttgtctgtt caaaatacaa
     9241 cgggatcgag tcgctatttt tgttggaaac agctaactca tcaattgtta aaggatattc
     9301 aaatctcaat ataaaataaa aagaaaaatc aggatcttct gaaccagcaa aatgtgtgat
     9361 ttttccttcc actgtgtaag gatctataac aaagagctga tggcgtcccg gaaaggataa
     9421 aagtaaaccg ctgtcatttt tgctgtaatc aatagataag attccgccat acatgctagg
     9481 tatcaatgta gaacagatgc cataacgtaa ttgacagatc gacagatgag tcgggcaaaa
     9541 tatactttct tctgggcggt aagaactttg agcatggaaa agggtgttct cctgtagttt
     9601 tccattgatc ggcgtaaaca gcatatgact gaaatcaccc atccaaggac ttggttgatg
     9661 ggtaagacgg tagccttgaa aaacgcggtc atcaggatga aaccaccagc tgccgttttg
     9721 atccgtagtt tgcggtgcaa aataattcat cccaaaagga acgccagtat aaggctggca
     9781 gttcccatta gagaaatttg gctgattcgc tgttgcatgt ctagtatcta tttgaccgat
     9841 attcatttta tttctccttt gttgattgtc ttttgatgaa tgaaactgga aatttactat
     9901 cggatggtcg atcttttgtc tctaaccaag tgaccaaagc atctcccgca agcttaccta
     9961 actgttcaaa atcttgtgcg atggttgtca gtggcgggtc aacaagagct gctgcttgga
    10021 tatcatcaaa tccgataatt gaaaaatctt cagggatatg ataaccatgc tgtctcaaga
    10081 gattcatggt ctggatagcg acaacatcgt tttcacaaac aaaagcagtt acttcatact
    10141 tagtgatata agaaagcaat tcatctaatg tcgcgctttt gtcatccagt gaagtgtgga
    10201 aagtcaaacc agctttgtcc aatgcttcaa ggtaaccaag gtaacgctgt cgaacggatt
    10261 gtggatgagt ctgcgtacca aacaaataag cgatttttga atgtccctgt tcgatcaaat
    10321 aagaggcagc aagcgcaccg ccttggaaat tatcggaaag aacggtagga aaaggtaact
    10381 catagatttt tttatctaat atgacgacag ggaattcttt caatgaaagc tcaaaaagta
    10441 agtcaagatg ttgttctgta tcaagagcat aataaatcaa tccgtcaaat tcattgatta
    10501 gctcttttgc tgttttattt tgtagaaagt caagcgtcgt catcatgaca tcaatctgtt
    10561 tatcttgcat cacagggttc aatcccttgc taaaatcacc aacagacaaa tcagcgacat
    10621 atggaagaag aaacagaatc cgtcgtgttt ttgtaggaga agcaagaatc tgttccggag
    10681 aagcggattt aacgaaactg cctttcccgc gaacgcgata aataagtcct aattgctcta
    10741 attcagtcaa tgcacgtttg gaagtgattc tgcttacttt ataggtacgt gaaagttctt
    10801 tttcagttgg tagttggctg tctacaggta attcaccagt taaaatagcc gtttgcaaat
    10861 cattaaatat tgtctggtac aatggttgat tcatataaac acctcttaat gatatatcat
    10921 attcaaataa taccataatg gatgaaaaag taaataaaaa gatatttttt gaaagcgtag
    10981 acataattgg ctgtatgaag tataatcttt aaagatatat caaatagaaa gaaggtttta
    11041 ttagtggctt ataaagatat accaaaatcg gtgcagaaat ttatggaaga tatcacacaa
    11101 aaatgtgggg atatccatgc ggactgggca aagaatttta cagcagcatt tgccaatact
    11161 ctgttgacaa cagtcaaaaa gcaaaatgac ggaaccacct ttttattgac gggagacatt
    11221 ccagcgatgt ggttaagaga ctctactgca caagttcgtc cttatttagt gattgcaaaa
    11281 gaagatgagg aattagctga catgatcagc gggttagtca aaaagcaatt tttttatatc
    11341 aatatcgatc catatgcgaa tgccttcaac gaagaagcta acggtgctgg ccaccaaaca
    11401 gatcatacaa aaatgaacga ttggatctgg gaaagaaaat atgaaattga ttctttatgc
    11461 tacccaatac agttggccta tcttctttac aaaaatacag gaaaaactga tcaattcaat
    11521 caatctttta ttgaaggtat caaaaaaatc ttggacgtgt tcaaaacaga gcaagatcat
    11581 gaacactctc cttatacatt tgtacgagac acgacaagaa aagaagatac gctgatcaat
    11641 gacgggcgag gtgcaaaaac agcttttaca ggaatgacat ggtctggttt ccgtcctagc
    11701 gatgatactt gccgttatgg atatctggta ccatccaata tgtttgcggt ggtcgtgtta
    11761 ggttatgtgg aagaaatttt tcaaacgata ctcgatgata aagaaattgt acagcaaaca
    11821 cagaaattga agaaagagat ccaagagggg attcagaatc acggacgaac ggttaatcaa
    11881 aaagggcaaa cgatttatgc atatgaagta gatggaatgg gaaacgccac agtcatggat
    11941 gacagtaatg tgcccaatct gatcgccgca ccttacttgg gatattgttc tgaacaagag
    12001 gaagattatc tgcaaacaag aagaacattg ttgagtaaag aaaatccata tttctatgaa
    12061 ggaaagtacg caaaaggaat tggatcctcc catacgccag aaaaatacgt ttggccgatt
    12121 gcattagcta tggaaggaat gacaacgtca gacaaatctg aaaaagagcg gatattgaat
    12181 ttacttgtag caactgacgc aggaacacac cttatgcacg aaggattcga tgtgaacaat
    12241 cctgaaaatt atacgagaga atggttttct tgggcaaata tgatgttttg cgaattagtc
    12301 atggattact ttgatattag agtagaaaag taggaggtat cgtaaatgaa aaagaaagta
    12361 tatatcattt ctcatagcca ctgggatcgt gaatggtaca tggcttacga acagcatcac
    12421 atgcgactga ttgaattgat agacgatcta ctagagctat ttgaagttga tccgtccttc
    12481 aacagttttc atttggatgg gcaaacgatt attttagatg actatcttca agtaaggcca
    12541 gaaaaaagac aggcaatcca acaagcaatc aatgaaggaa aattgagaat tggaccattt
    12601 tatatcttgc aagatgattt tcttatcagc tcggaatcga acgtgagaaa tatgctgatt
    12661 ggcatggaag aaagccgtaa atggggaacc cctgtcatgt taggctattt tcccgataca
    12721 tttggaaata tgggacagac gcctcaatta atgaaacaag ctggtatttc agcagcagcg
    12781 tttggccgtg gtgtaaaacc aattggcttt gataatcaag ttttggaagc agaaaactat
    12841 tcttcccaat actcagagat gtggtggaaa ggacctgatc aaacagcaat ctttggtctg
    12901 ctatttgcca attggtatag caacgggaat gagattccag tagaaaaaga agcagctctt
    12961 gccttttgga agcaaaaatt agcggatgca gaacaatatg cctctacgaa ccacttattg
    13021 atgatgaatg gtgttgatca tcaaccggta caaaaagata tatcaaaagc aatccatctc
    13081 gcaaatgaac tgtttccaga ttacgagttt attcattcta attttactga ctatctggaa
    13141 gcagttcaaa aagatgtgcc agaagactta ggctcagtag aaggagaatt gacaagtcaa
    13201 gaaacggatg gctggtatac tttagccaat acagcatctg ctcgggttta cttaaaacaa
    13261 tggaatacca aggtccagcg acagctagaa aatattacag aaccacttgc gacgatggct
    13321 tatgaagtta gtggaaacta tccccatgat caattagatt atgcttggaa gacattaatg
    13381 caaaatcatc ctcacgacag tatttgcggt tgttcagtcg attctgtcca tcgggaaatg
    13441 atacctcgtt ttgaaaaagc agatgaagtt ggaaagtatc ttgcacaaga ttccttagaa
    13501 cagctgaccg cagcaatcga tacgaccgga ttcccaaaag acagctttcc atttgtcatt
    13561 gtaaatactg caggaatgga taaaactggt gaagcagaaa taaccatcga attagaaaga
    13621 aaaagattcg ctgaaggaat acctgaacaa ctatatcaag agttagaaaa tttgcctaaa
    13681 cgtaagtatc atgtagaaac aaaaagtggg gccactatac cagctatttt atctgaagaa
    13741 actgtccaat ttggttatga tttgccgaaa gaccgtttcc gtgtgccata tatggcaaga
    13801 atgatcaaag tgactttacc attagaaaac atgccagcat tttcttggga aacattcgcg
    13861 cttgtagaag gggaagcgaa agcagaagaa aaggaaacaa tgattcatca gagtggacga
    13921 atcatcgaaa acggccctct acatttgacg attgaaaaaa atggtaccat tacaatggaa
    13981 gatcgcaaaa acaaacgaaa gttgaataat ctgcatattt ttgaagatat cggcgatata
    14041 ggaaatgagt atatctttaa acaacctttc tgtgatcagc caatcctatc cagtaacaaa
    14101 gaaaactctg aagtaaaagt attagtagat acaccggaaa tagcaaaaat aagtattcta
    14161 caggaaatgg aaatccctgt ttctgcagac gagcgtttag aaaaagaaca gcagatggtt
    14221 gtggaatttc gttatcgaaa agcagaacga tccaaagaaa aacgaatcct tcaaataaaa
    14281 acaatcatga caatcagaaa agatagtaaa aaaatcgact ttgaaacgac tctcgacaat
    14341 cagatgaaag atcatcgctt acgtgtcttg tttcctacaa agttacatgt ggaacatcac
    14401 gaagcagata gtatttttga agtggtgaag cgcccaaacc atgtgtctaa aagttgggaa
    14461 aaccccacga atccgcaaca tcagcaagca tttgtcaata ttcatgatga agagtacggt
    14521 gtaacagtcg gaaactttgg tttgaatgaa tatgaagtaa cagaagacgg acaaatcgca
    14581 gtgacgctac ttcgcagtgt gggagaacta ggcgattggg gatattttcc aacaccagaa
    14641 gcacagtgtc taggtgagca tcggttcaat tacagcattg aactacatgg accagaagaa
    14701 aaattctcta cttatttgca tgcatacgca gcacagattc ctttttccac tcaacaaatc
    14761 aaacatcatg aaggaacatt gatttcaaaa caacaatatt taacgataaa aagtgaaacg
    14821 tttgcaatta cagctttgaa acgcagcaag tttagtgata aagtagtagt aagaggtttc
    14881 aatatgtcta gtcatttaga aaaattggaa ataactaaag acaatggaaa aactgtcata
    14941 ttgaatctgc ttgaagaacc aacgaagcaa gcggttgtcc caataatcca accttatgag
    15001 attcggacaa tcggattcga ggaggaaaac gaatgtatgg cggaatagaa gcaggcggaa
    15061 caaaatttgt gtgtgcggtt tcagatggtg gagaaatttt agaa
//
LOCUS       DAYYBH010000060        14716 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000060 DAYYBH010000000
VERSION     DAYYBH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.062"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(510..1076)
                     /locus_tag="ACHAUG_002104"
     CDS             complement(510..1076)
                     /locus_tag="ACHAUG_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8481186.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="ACHAUG_002105"
     CDS             complement(1076..2032)
                     /locus_tag="ACHAUG_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8481187.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="ACHAUG_002106"
     CDS             complement(2032..2688)
                     /locus_tag="ACHAUG_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8481188.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="ACHAUG_002107"
     CDS             3058..5076
                     /locus_tag="ACHAUG_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8481189.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5146..5352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5503..7917
                     /gene="leuS"
                     /locus_tag="ACHAUG_002108"
     CDS             5503..7917
                     /gene="leuS"
                     /locus_tag="ACHAUG_002108"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8481190.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="ACHAUG_002109"
     CDS             8265..9818
                     /locus_tag="ACHAUG_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8481191.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="ACHAUG_002110"
     CDS             complement(10199..11983)
                     /locus_tag="ACHAUG_002110"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8481192.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="ACHAUG_002111"
     CDS             complement(11998..13719)
                     /locus_tag="ACHAUG_002111"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8481193.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..14580
                     /locus_tag="ACHAUG_002112"
     CDS             13819..14580
                     /locus_tag="ACHAUG_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8481194.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4774 a         2725 c         2912 g         4305 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaattcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    14581 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    14641 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    14701 tttttagagg aaatat
//
LOCUS       DAYYBH010000061        14624 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000061 DAYYBH010000000
VERSION     DAYYBH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.063"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..815
                     /locus_tag="ACHAUG_002113"
     CDS             3..815
                     /locus_tag="ACHAUG_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8481195.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="ACHAUG_002114"
     CDS             812..1612
                     /locus_tag="ACHAUG_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8481196.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="ACHAUG_002115"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="ACHAUG_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8481197.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="ACHAUG_002116"
     CDS             3109..4818
                     /locus_tag="ACHAUG_002116"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8481198.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9388
                     /locus_tag="ACHAUG_002117"
     CDS             5030..9388
                     /locus_tag="ACHAUG_002117"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8481199.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9533..10006
                     /gene="rimP"
                     /locus_tag="ACHAUG_002118"
     CDS             9533..10006
                     /gene="rimP"
                     /locus_tag="ACHAUG_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8481200.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10029..11204
                     /gene="nusA"
                     /locus_tag="ACHAUG_002119"
     CDS             10029..11204
                     /gene="nusA"
                     /locus_tag="ACHAUG_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8481201.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11226..11519
                     /locus_tag="ACHAUG_002120"
     CDS             11226..11519
                     /locus_tag="ACHAUG_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8481202.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11516..11827
                     /locus_tag="ACHAUG_002121"
     CDS             11516..11827
                     /locus_tag="ACHAUG_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8481203.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11840..14146
                     /gene="infB"
                     /locus_tag="ACHAUG_002122"
     CDS             11840..14146
                     /gene="infB"
                     /locus_tag="ACHAUG_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8481204.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14173..14520
                     /gene="rbfA"
                     /locus_tag="ACHAUG_002123"
     CDS             14173..14520
                     /gene="rbfA"
                     /locus_tag="ACHAUG_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8481205.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4982 a         2598 c         3255 g         3789 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5701 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5761 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5821 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     5881 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     5941 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6001 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6061 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6121 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6181 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6241 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6301 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6361 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6421 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6481 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6541 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6601 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6661 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6721 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6781 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6841 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     6901 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     6961 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7021 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7081 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7141 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7201 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7261 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7321 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7381 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7441 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7501 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7561 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7621 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7681 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7741 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7801 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7861 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     7921 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     7981 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8041 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8101 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8161 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8221 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8281 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8341 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8401 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8461 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8521 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8581 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8641 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8701 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8761 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8821 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     8881 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     8941 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9001 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9061 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9121 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9181 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9241 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9301 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9361 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9421 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9481 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9541 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9601 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9661 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9721 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9781 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9841 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
     9901 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
     9961 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10021 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10081 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10141 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10201 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10261 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10321 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10381 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10441 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10501 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10561 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10621 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10681 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10741 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10801 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10861 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    10921 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    10981 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11041 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11101 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11161 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11221 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11281 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11341 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11401 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11461 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11521 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11581 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11641 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11701 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11761 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11821 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    11881 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    11941 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12001 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12061 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12121 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12181 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12241 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12301 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12361 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12421 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12481 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12541 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12601 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12661 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12721 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12781 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12841 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    12901 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    12961 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13021 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13081 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13141 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13201 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13261 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13321 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13381 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13441 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13501 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13561 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13621 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13681 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13741 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13801 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13861 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    13921 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    13981 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14041 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14101 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14161 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14221 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14281 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14341 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14401 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14461 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14521 tcaaaaactc ccgcaaatcc tgtgaaatca ggctctttgt caataaggac tgatgagttg
    14581 tgcaaaatca aatctgagtc agaatgaacc acattctggc tcag
//
LOCUS       DAYYBH010000062        14612 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000062 DAYYBH010000000
VERSION     DAYYBH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.064"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(214..2289)
                     /gene="glyS"
                     /locus_tag="ACHAUG_002124"
     CDS             complement(214..2289)
                     /gene="glyS"
                     /locus_tag="ACHAUG_002124"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8481206.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2291..3208)
                     /gene="glyQ"
                     /locus_tag="ACHAUG_002125"
     CDS             complement(2291..3208)
                     /gene="glyQ"
                     /locus_tag="ACHAUG_002125"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8481207.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3594..4412)
                     /gene="recO"
                     /locus_tag="ACHAUG_002126"
     CDS             complement(3594..4412)
                     /gene="recO"
                     /locus_tag="ACHAUG_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8481208.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4555..5454)
                     /gene="era"
                     /locus_tag="ACHAUG_002127"
     CDS             complement(4555..5454)
                     /gene="era"
                     /locus_tag="ACHAUG_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8481209.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5469..5864)
                     /locus_tag="ACHAUG_002128"
     CDS             complement(5469..5864)
                     /locus_tag="ACHAUG_002128"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8481210.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5848..6324)
                     /gene="ybeY"
                     /locus_tag="ACHAUG_002129"
     CDS             complement(5848..6324)
                     /gene="ybeY"
                     /locus_tag="ACHAUG_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8481211.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6353..8545)
                     /locus_tag="ACHAUG_002130"
     CDS             complement(6353..8545)
                     /locus_tag="ACHAUG_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8481212.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8569..9540)
                     /locus_tag="ACHAUG_002131"
     CDS             complement(8569..9540)
                     /locus_tag="ACHAUG_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8481213.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10245..11624
                     /gene="brnQ"
                     /locus_tag="ACHAUG_002132"
     CDS             10245..11624
                     /gene="brnQ"
                     /locus_tag="ACHAUG_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8481214.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11906..13246
                     /locus_tag="ACHAUG_002133"
     CDS             11906..13246
                     /locus_tag="ACHAUG_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8481215.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            13403..>14612
                     /locus_tag="ACHAUG_002134"
     CDS             13403..>14612
                     /locus_tag="ACHAUG_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8481216.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVL"
BASE COUNT         4101 a         3096 c         2695 g         4720 t
ORIGIN      
        1 gaaattccta atttaaaagt aaataaaaca gtaacaagat tataattaat cgttttcatt
       61 tttatttcct cacttttcat gagcgttttg aaaattcgaa caaagaataa aataacaaaa
      121 attattaaaa tagaattaat aacagctatt tataacaaaa aaaacagaga cacagcacgt
      181 ttcgtgcatg ctctctgttt ttcaaacaaa agactatttt gtgatgattt gatccaagct
      241 tgctaatgac aaagccatgg cagctatttg acttagttgt gttagtcggt tcttacgtac
      301 tgctggttca tcagccatga ccatcgtttg atcaaaataa ttttcgatca gtggacgaag
      361 agcgacaagc tggtcgtagt tttcgtggat cgtttggtca gcaaaacttg ctttcattgc
      421 ttctactgct ttgtgtaatt ctttctctgc gtcgttttcg aacagctcag ggtctacttc
      481 tgattgtttt tcaagctgcg cttttttcgc taagttcacg acacgtgtca aagcttccat
      541 tgaaggtttg aagtcttcat ctgcaagatg ttgtttgaac aattgtgctg ctgcaaatac
      601 ttttgtcaga tctttttgtt ccgcattcaa aacagcatcg atgacatcat gacgaatatt
      661 cttcgttgtc aataattggc gcagacggcc tttgataaag tcgatcactt gttgttgccc
      721 ttctgataaa ccaatgccga aacgatccgt atctttactg ataacatcgc taatcgtttc
      781 ttgcagaaca gacaaaggaa aggcccattt tttcgattcg atgatccgaa cgacaccgta
      841 tgcttggcga cgtaaggcat aaggatcgtt tgacccgctt ggaatcatcc caactgcaaa
      901 gaaagaaaag atgctatcta atttatctgc aatcgctaaa acagaaccta ctgtagactg
      961 aggtagctct ccttcactgg atataggcat gtaatgctca cgaatcgctg tcgctacagc
     1021 aggtttctcg cctttcagaa gagcatattt ttcgcccatg atcccttgta gttcaggaaa
     1081 ttcccctacc atgttggtta ctagatcaaa tttatagatt tcagcagctc gttgcaaatc
     1141 catcaattct tcctcagata gtcctacttg ttcgccaatg atacgggcaa tgacttgtac
     1201 ccgttgcatt ttctcataga tactaccgat tttttcatgg aaagtaacag tttttagttt
     1261 ttcaacgtat tggtcgattg aagctttctt gtcttcatta taaaagaatt ccgcatcttc
     1321 taaacgagcc gtcaatactt tttgattgcc tcgaaccaca tcttccaatt ttacccgatc
     1381 accattacga gcagaaataa aatgaggaag cagcatacct tgatcattcc gtacctcgaa
     1441 ataacgttga tgttctttca tagaagtgac tagtacttct tcaggaacag aaagatattt
     1501 ttcttcgaag tcgccagaaa agacagttgg atattcgact aaattgttga cttcttctag
     1561 taaatcttcg tctagatcaa taacccagtg atttttttct gctaattcgt ttgcttgtgt
     1621 aacgatcatt tcttgtcttt tttgcggaga cgcaatgacg aattgttcat tcaatttttc
     1681 ttcatattct tttgcatgcg caaatgtcac atcttcgcct aagaaacgat gacctcgaga
     1741 ggctctgcct gttttgacat ccaagatttc gaatgggatt acttcgtcat ctaacaatgc
     1801 cacgatccag tgaatagggc ggatatattc aaaatcgtat tctgcccaat gcatcgttac
     1861 agggaaagtc aaacttttga tcacccctgc taagcctgtc aatacttctt ttgcttcctt
     1921 accatgagtg tatttcgtta catagacata ttccacacct tttaattctc taaaagtgat
     1981 agcgtctgtc gtcagacctt gtccgcggac aaatccttcc gctgctttcg tccagtttcc
     2041 atctgcgtcc aaagcaattt ttttggctgg tcctttgact tcttcttcac tgtccgcttg
     2101 tcgttctgga atatttgtta ctttgattgc taaacggcgc ggtgtggaaa atgtttcgat
     2161 cgtatcataa gtcaattggt tttcatctaa aaattttgct gttttttctt ctaattgttt
     2221 catgcttggt gtgacaacat gggcaggcat ttcttccagc ccgatttcaa gtaataagtc
     2281 ttttgccatc ttattcagcc tccttcacgg tttctggatt tgctgtgttt ttattcaaca
     2341 aagggaatcc taatttttcg cgttctgcca caaagatttt ggctacagca cgagccatgt
     2401 tgcggatgcg agaaagataa ccggcacgct ctgttacgga tactgcaccg cgggcgtcta
     2461 ataagttgaa tgtatggcta catttcaaga tataatcata cgccggatga acaagatttt
     2521 catcgataca gcgtttagct tctttttcaa aacgatcaaa gttgcttagt agcaagtctt
     2581 ggtcgcttac ttcaaaagaa tatttggaat gttcatattc tggttgtttg aagatctcgc
     2641 catatttcac gccatcagcc cattctaaat catagacact ttcaacttct tggatataag
     2701 aagcaagacg ttctaatcca taagtgatct cagaagttac tggtttacaa ggcagtccac
     2761 caacttgctg gaagtacgtg aattgtgtga tttccattcc gtcaagccat acttcccaac
     2821 ccacaccggc acagcccatg gatgggttct cccagttgtc ttcaacaaaa cggatgtcgt
     2881 gttctaatgg atcgattcct aaaaggcgca agctttctaa ataaagctct tgaatattct
     2941 caggtgaagg tttcatcacc acttggaatt gatgatgctg atacaaacgg tttgggtttt
     3001 caccataacg tccatcagct ggtcggcgag atggctctac gtaagcagca ttccatggtt
     3061 ctggtccgat tgcccgtaaa aaggtatatg ggctcatcgt acctgctcct ttttctgtgt
     3121 cataagcttg catcagcatg cagccgtttg atgaccaaaa tttttgtaat gttaaaatca
     3181 tatcttggac ggtcaatttt ttactcatct gttttgcccc tttcaatttt aaagcgttgc
     3241 tgtcaaataa gaatacagga aagaaaacga caaaaaatcc ctatgtctga ttacagacat
     3301 agggacgaat aattcgcggt tccaccctac ttccgaataa agattcggca gcttcattta
     3361 tcgtactcgc aagtgcctct tcaaaaatcg tttgggtccg gctcccactc tcccgaactc
     3421 gctaaaccac gtgtttttta ctgatcttgg tcaatgtacg tattcatttt gcagttttct
     3481 tgtttctata atagatccta tcactgaacg atctatttaa tctatggtta ataatagacg
     3541 gaactccctg tgtttgtcaa atggttttga ttttttgttg tatgttttat ttactattct
     3601 tctttttcag gtgtctcttt ttctttttca gacatgtctt tctctgacgt ctttaatacg
     3661 tgtgcccaag acttcatttg attgatgaac tttttgcttt tcaaatggat accgacatat
     3721 tcatcataca gctggtcgat gagttcacgg attgcttgtt tggtttcttc tttcagatgg
     3781 attccactaa ttttatcata agaaatagct gaaaacatcc gaacgaaata aacagctcga
     3841 ggatctgcat gataacggtg atcgtccatc gaccaatgtc tttcgcatat cacaccacca
     3901 tatttggaag aatagtcaaa ttttcctttt gtttccccgc atacgacgca gtgtgtccag
     3961 attggtgcaa ttccgaagcg ttgcagtatt tggacttcaa aaatattcgt gatgatttct
     4021 gcatcgtctc cttggtccat ccgttgcagt gcttgttgcg taaactggaa caaatgcggg
     4081 tcgtactgat gatcttcgat tgccgcatct actagattca ataaatatgt gccatagccg
     4141 ctcaaaaaga tgtcttcttg gatgtgtcga aacgcatgga catctttgac actgcttaaa
     4201 aatgacaaac cttcacttcg aaaattcccg atataaactg cttcagtaaa agggagcaaa
     4261 gccggagcta agggctgatt ttgccgatgg atctctttga tataaaacat cattttgcca
     4321 gaagattctg taaagatttt gactaattta tctttttctt tgtaattacg gctagataaa
     4381 atcaagcctt ttgattctgc aagctggctc atattctccc tcctaaaaaa taaatctgga
     4441 aaagcagatt ctgccgatcc agatcattta tgttgcgatc ttccgcagct tttctcaaca
     4501 cgcggatttt tattcagttt atttctatac ctaatttaca agaagctcag aatatcagta
     4561 ttcgtctttt cggtagccga agtcttgcag atagactttt ttgtctcgcc agtctttttg
     4621 aactttgacc caaagttcta gaaagacttt gtctcctaaa agattttcga tatcttttct
     4681 tgctttcgtt cctacttgct tcagcatttt tccgcctttg ccgataatga tccctttttg
     4741 gctatctctt tcgacaataa tcgtagcctg gatatggatt ttatcattct cgtttcgctt
     4801 catcgaatca acaacgactg cgacagaatg aggcacttcg tctcgtgtta aaaacaagac
     4861 tttctcacgg atcaattcag aaacgatgaa gtattcagga tgatcagtca cttgatcatc
     4921 tggaaaatat tgtgggccct ctggcatttc actgactaat acatccatca aacgttcgac
     4981 gttgtttcct tctgtagcag agattggaat cacttgtgca aaaggcatct gcgttgaata
     5041 atcttcgatg attcccaaca actcgtctgg atggattttg tcgattttat tgataatcag
     5101 ataaacagga gaatgcacgt tttttagacg ttcaataata aaatcgtctc cccgaccgcg
     5161 tttttgatca gcactaatca taaataagac cgcgtccact tctttcaacg cactgtaagc
     5221 tgcttctacc ataaaatctc ctaaacgatg ctttggttta tggattcctg gtgtatcaat
     5281 aaaaatcaac tgagcttctg gtgtagtgta tactccttgg attttatttc tagttgtctg
     5341 tgccttgtca ctcatgatgg caattttttg acccacgatt cgattcaata atgtggattt
     5401 ccctacattt gggcggccga cgattgcgac aaaccctgat ttatgttctg gcatatatgt
     5461 tctccttttt aattaaacaa caaatgatat attttaggga caaataaaat caacccaacg
     5521 atgacggtaa aacaagcggt cactaaaact gctccagctg ccatatcttt gatttttttc
     5581 ccaatcggat gaaaatgaaa atccgtaaac atgtccacga cattttcaaa aacagtattg
     5641 atgatctcta ctatccatac gagaaaaaca gctaaaagca gccataacca ttctatccga
     5701 tcaagttgga aaatcacgcc cataaacaaa gcgaggattc cgaacgcaac atgtttcctc
     5761 atgttgcgtt cttcctcaaa aacagttttg actccttgga cggcaaattc cacggacatc
     5821 aggaaatgtt tgtttttctc tacgttttta tcttttaagt ccataggcat ctaagatctc
     5881 tttctgtaaa ccaaacattt ctttctcatc ctctggtgtc atatggtcat acccgttgat
     5941 atgcaagaat ccatgaaccg ctaaaaagcc aagttcccga tcatacgaat ggccgtaagc
     6001 ttctgcttgc tctgcagcac gttcagtaga gatcatgatg tctcccagta cacgaggcaa
     6061 tggattctct ccatcttcaa aaatcactgg gatttcgtct tcgccctctt cttccaaggc
     6121 aaaactgatg acgtctgtcg gtgcatcttt tccacggtaa tcacgattga ttacttggat
     6181 tgctgcattg tccatgaaag taacagacat ttctgtttct tctggcaact ctaaatatga
     6241 tgctgcaaat tgcagcaagt cgtcgatttc tttcattttc tcttctgaga cttggtttgt
     6301 ttcatccatg aaggtaatat ccattataca tcgcttcttt ctattcttct tgctattctt
     6361 cttgttcttc ttttatcttc tcaacttccg atttcatttt aggatatttg atccgagaat
     6421 ggaaggtact gcttaaaccg ctgatcaatg atttttctac tttacggatt tcttttaacg
     6481 tcaatccact gtcgtccaat tgtccatcgg aaatacgctc ttcaataaga ttatggacaa
     6541 aactttcgat tttttcgctc gttggatggt ccattgcccg tactgccgct tcacaactgt
     6601 cagctatatt gacgattcct gcttcacggc tttgaggttt tggtccagga taacggaatt
     6661 gctcttccgt aacttccggg ttgcgttcct ttgccttaac gtagaaataa cgcatcagcg
     6721 tggttccatg atgctgtcta caaatatcga tcaccatttg cggcatatta taatcttcca
     6781 atattttggc accatctgtc acatgtccaa agataatatg tttactgtct tctggcaata
     6841 agaaattgtg cggattttcc gctcctgaag gcaagttctc aacgaaaaag ctggcgtgtt
     6901 tgatcttgcc gatatcatga taatagcaag ctactcgagt tagtaaagaa cgtccaccga
     6961 tttctgctac agcattagca cttagattgg ctaccatcat gctatggtga tacgtacctg
     7021 gtgcttcttc cagcaactgt ttcaataaag gatgattggg attgctgagt tcatttaata
     7081 cgatcacact atcatcattg accatcaatt caatataggg gtgcagacct atcgtcagca
     7141 agaatgaaaa cagtgcacct gtgaatccgc agactagcgt taaccaagtc gttgcatccc
     7201 caaagctcat tccttggtag ataatcaaaa tgacatccat catcacagga aaaataatga
     7261 cccacatagc agcggataac ccttgttctg agatccgttt gcgtctgacg atcgttgcca
     7321 tgagaccaga aaatacatag gacatcaaaa taactgtcag aaagttcgtg ccaatcgaat
     7381 cataaaaaat gaaaagtgcg gatactgctt gaaaaatacc agccatgatc cctgctcttc
     7441 gattcaagaa aaagcttaaa acaagtggag caaaagccgc ggggtaaaac agcggaatat
     7501 aagcagcttg ttctgtttgg aagagttgga aaaatttcat tagtacgacg cttactgaca
     7561 ttgccgtcac gtagaaaagt acgtattttg ttctttctcc tgcattttgg aattgaagtg
     7621 aattataaat cagtacaccg atttgaagca aaacagctaa aaccatggca atgagtggga
     7681 aaatagaagt cgtttgattc gtcaatccta gtaattccaa ttttttcatc gctgaagcat
     7741 caatttgatt tccttcgcga acaataattt ctccttggaa gatcatcact ggttgtacag
     7801 cttctcgtgc agattgtttt aattcttctg tctttttctc attcaataca tcattaacta
     7861 cgatgccttg atccactaag tagcgaatca tttgttgctg ctctgttgtg acattcaaat
     7921 attgaatctg atcttctgct tcttgtttaa aagcttccaa ttcgctttca cgaacttgtt
     7981 tactcatctg ttcgtcaatg agcttcaaac tttcatccct aactgtgtga atttccgttt
     8041 cagtcatttc aaatatcgtc gaataaaaat tgttcggcag tttttggtaa aacgcaacgt
     8101 tttctgcatt cacgttttca aaatttttct tcagtgtagc aatccgctct tctgtagtag
     8161 gtttcgggat attttcatct tccttcgcct tatctacctt gccattataa gatttgttga
     8221 tggtatcatt cgtcttatcg atcaattcga ataattgctt gatccgattg ttctggtcat
     8281 ctgccagatc tttttgataa gtatattctg gagtcactgc ttctgctgcc agttttcttc
     8341 tttgttcggt ttcttccgtg ttctcaatcg ttttattcgc acggatactc tcttctgcta
     8401 cttggccttc tctgaaatca atggcctttt gtttgacact actaaatgta acgccgcaga
     8461 caatgataaa aaacaagaac agaaggaaag gcaatgctgc ctttccgagt ttttcttgta
     8521 gtttctgcag tgttttattc aacatgtgct tatcctccta agcaaagctt attcttgatg
     8581 aagatccgcc ttttcataag ctttgatgat ttcggcaact acagggtgcc gaacaacgtc
     8641 acttgcttca aaattaacaa aatcgatttt tttgatttgc tttaatgttc gttctgcatg
     8701 aacaagacca cttgtcgtcc cacgaggcaa gtctatctga cttgtatccc cattgacaat
     8761 catttttgat tgataaccga gacgggtcaa gaacattttc atctgagcga ctgtcgtgtt
     8821 ttgtgcttcg tccaagataa cgaatgcatc atccaatgtc cgcccccgca tgtaagctaa
     8881 aggtgcgatt tcgatcacgc ctcgttccat caatcggttc gtatgatcca atccaaaaat
     8941 ttggtataaa gcatcataaa caggccgcag ataaggatcc actttttctt tcagatcccc
     9001 gggtagaaag cctaggtttt cccctgcctc cacagcgggc cgcgtcaaaa tgattttttg
     9061 cacttcgcct ttttttaggg cagcaattgc taatactacg gcaagaaacg ttttccctgt
     9121 tccggctggc cctacaccaa atacgatgtc atgtttggct accgattcaa cgtatttctt
     9181 ttgaccgctg ttttttaccc gtataggttt tccattacgg tctttgacga tctcttcctc
     9241 atacatctca acaaaataat ctaatgtacc tttttgcccc attttcaatg ccgtcacaac
     9301 atctggcgta cttacgtgga ttcctcgttt gatcaattcc tgtaatgtcc gaatgactga
     9361 ggcagccaag gaaatctggg attgctcacc aatgatctga atgatttctc cacgtgtatg
     9421 gatggttgtt tccgttgttt cctcgatcag ttttatatgc ttgtcgtgtg cacctaaaag
     9481 catgctgaca tcatcagcag ctgtcaatct aatctctaaa gaactagact cttccgtcaa
     9541 aaatagacat cccctttttt tcgattcatt ctttcctatt ttaccatact cagaagaaac
     9601 tatatagcaa ataagaaaag gttgtaaaag agtcgcttaa ccatacgtac caagaaacgt
     9661 taaaaaatag ccgtttccat tcgctaaagg tctcgttttg ataaaaagaa gctgagaatg
     9721 taagttatat gaaagtggag aacaaaatat ttcgtttcat tcaacaagta ttctctaggt
     9781 atttttcctg tttcaaaaaa agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa
     9841 aacaggtgta ttattacgtc aacaaataaa catacagcaa agcgtcgagg aaaacagtag
     9901 gctattcaac gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata
     9961 gacgaacatc atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat
    10021 caggctagcg gataacttta tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa
    10081 aaaaggtggt accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt
    10141 tattttaagg tggtggtttt catggaatct gatattagtg gcattactaa ttgctgcaac
    10201 gtttatatcg tcaatcaaaa tgataattat tggaggaaaa atttatggaa gaaacaaaaa
    10261 cttattcatg gaaacaacta ttgatggtca gttcacttat cttcgggatg ttcttcgggt
    10321 caggtaatct aatctttcct gtccacttag gtcaaatggc tggcgcaaac tgggtcagtg
    10381 ctggcgtagg atttgctatc tcaggtgctc ttttcccatt actagctatc ttagctgtag
    10441 tagtgacaaa aagtgatggt ctgtatgact tagctaaacc tgtcggaaaa tggtatgctg
    10501 ccttgttctt aatccttgtt catttgacga tcggaccatt tttcggtaca ccacggacag
    10561 ctgctactgc ttttgaaatg gctgctaaac catttttgcc tgaaaaatat aattcattag
    10621 gtatgttttt attctctgct cttttctttt tacttgctta tttccttact gtccatccaa
    10681 cacggttggt caaatacgtc ggaaagtatc taaactcctt gtttcttgca ttattagcaa
    10741 tcatttttgt cgtttcttta ttccatccaa tgggaaatct aaatcaatct gctgcagata
    10801 cttatcaaac aaacgcttta acaaatggtg tcctagaagg ttataacacg gttgatgcag
    10861 tagctttgct tgccttaagt gtcacttttg tccatgcagt ccgtgacctt ggtttcaaag
    10921 ataaaaaagt cacagaatta acggcaaaag ctggtagcct gagcgttgcg ctagaagtca
    10981 tgatctattt tggacttgtt cttctcggtg cgatgagttt gaataagatg tctttatcag
    11041 aaaatggcgg aactgcactt tctcagatca ctaattttta tttaggtaac ttcggcgcta
    11101 gcttcttagg agtgatggtc acattaggtg tcttcactac cgctatggga ctcgttgttt
    11161 cttttgcgca agatttccat aaactgttcc ctaaagtcag ttatatgaca tggctgcggt
    11221 taacgacttt cgtctctttt gtcgttgcaa atgctggttt agacaatatc atccaatggt
    11281 ctttaccagt attgatgctt ttgtatccat tgtcgcttgc gttgatcttg ctttccttaa
    11341 cagcaaaatt tttccaaaag acaccatttg tctatcaagt aacgatgttg tttgctgctg
    11401 ttcctgccgt attggatatg ctggcaaatt cgcctgcttt agtgagtcaa caaagagttg
    11461 tagcaagcat gctcgaattt tatcatcatc acgttccttt tgctgcatta ggacttggct
    11521 ggatggtccc aacgttactt ggttacgctg gcagcctact gttttattac gcctatcgcc
    11581 tatcagggta taaacaagaa gcaaatgaac taccagaaga ataaccaaat caaacacaat
    11641 ttaacataag aaatattaaa caggaaaaaa ccagctagat gaaagaatcc tattctttcg
    11701 tctagctatt ttttagttct cttttttacg cgcacaaaaa aactgcggac aatgattgtc
    11761 cgcagtttaa atgggctcta taatatctag tgttggtgac gccttctgtt gccaatactt
    11821 tttctttttt ctaataaacg caaacagaca ccttgaaatc ctttagaata aagtcgacga
    11881 aaaccattca aaggagtgtt tcaagatgtc ctattcagag tttaccaaag agttactaga
    11941 tattctagac ctaaatctta tctttcatga agatgccttt cgcaaagagc gaatcaatga
    12001 cgaaacatgt tttgtttttg atggcacatt gacctatcaa ccagaggaat gcttccattg
    12061 tcactatcaa aataagcaaa cgatcattaa atggggctgg aaaaaagtat ccatcttatt
    12121 gaatgatgtc agtaactaca agaccattct gcggattaac aaacagcgct tcaaatgtaa
    12181 acactgtgga aagacttttt tagcagaaga ttcagttagt gatcgtcgct gctccattgc
    12241 tcgcagagtc aaacaagcca ttcttgagct attaagcgaa cctctttcta tgtctttgat
    12301 cgcacgtatg aagcacattt ctccgaccac tgtcattcga atacttcgca gccttcggcc
    12361 taaaaccgtt tctttgaacc agccgttacc agaagtggtg tgtttcgatg aattcaaatc
    12421 ggtgaaaaac gtctcgggag ccatgagctt tgtcatgatg gacggcaaga cccatcaact
    12481 gattgatatt gtggaaaatc ggcaactaaa tcctttacgc gactatttct tgcgctaccc
    12541 tagaaaagtc cgtgaacaag tacgtctagt ggtttcagat ttctatacac cctatcgcac
    12601 attggtgaaa gagtgctttc cgaatgcccg aatcgtcgcc gatcgctttc atattagcca
    12661 acacatcggt cgggccttta ccaaccaccg aattcaagta atgaagacct ttaaaaaaag
    12721 ggatcgtcgg tcgaagcact taaagaaata ctggagacta cttcaaaaga atgcgtggga
    12781 attgaaaggt cagcatcgct actggcgtcc gtcatttcga gatcatctga cagaagcaga
    12841 gatagtcgat cggctattat cttatgatga ctccttaaag cgaggctacg aagtctacca
    12901 ggattttctt tccgctatca gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga
    12961 agactacaag gagttgccag agcactacca accagtgttc acgaccttca agaaatatcg
    13021 aacagaaatc aaacgagcgt tgcgagttcc ctattccaac ggccctatag aatgtttgaa
    13081 caaccatatc aaagtactaa aacgaattgc ctatggcttt agaaactttc aaaactacca
    13141 agaacgaatc tttttatatc gaggaaaata ttttaagaaa actaagaaca ctacccaact
    13201 gactaaagct agaacaacaa cacggctaga caagatagct gtctagccgt gtgagtatct
    13261 cgattattct attgaatttc ggctctatac tttttgatgt tggtgaactt caccaacatc
    13321 ttttttgtat aaaaaaatat agaaaagaca ccctgaaatc ctttagaatt aatttggcta
    13381 ccaattcata ggaggattca agatgtctca tactcagctt atcaaagata ccctaaatat
    13441 tctagaccta aatatctatt tcgaggaaaa ttgtttaact aaagaaaaat acaaagggca
    13501 aatttgcttg atttacaaag gaactcttct gtacaagcca gaagagtgtt cccattgtct
    13561 ttgtgtcgtc ccctctagga tcattcgttg ggggacgaca accgttcggt tgttactgaa
    13621 tgatgtgtct gagtatcgca cttatctgga gctgaagaaa caacgattta aatgtaagtc
    13681 ttgccaaaga acattcgtag cggatacctc tgttgctgaa aaacactgtt tcattagtca
    13741 aaaagttcgt tggtctgtca ttgctcgctt aaaagagaat acttccatga cggaaatcgc
    13801 tcgtcagaaa aatatttcca cttcttcggt ctatcgtgtc atgaagcgat tctaccgccc
    13861 attgaatcct ttcaaacaga ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt
    13921 tcgaagtgtt gccagtgcaa tgagttttat catgatggac ggacaaagcc atgcattatt
    13981 ggatattgtc gaaaatcgac gcttgcctta tttagaacgc tatttttctc ggttttcatt
    14041 agcgactcgt gaagccgttc aattgattgt tattgatatg tatgcgcctt atgtttcgtt
    14101 ggtgaaaaag cttttcccga atgctcagtt gatcatcgat cgtttccaca tcgtccaaca
    14161 tattggcaga actttcttga atcatcgagt gaaagaaacc acggtacgac ttaaaggaaa
    14221 ttcccactct caacgagggt tagggaaaaa attgaagcgc tattggaaac tcctacaaaa
    14281 agaagaagca aagttaaact atgaaaaacg ggtttggcgt gccagtttta aagattattt
    14341 gactgaaagt gagattgccg atcgactgtt agctgcttgt cccaacttac gccaaggcta
    14401 tcagttatac caagacattc tttatgcggt aaagaaaaga gatcagtcac tatttgagga
    14461 ttgtttaact agagacatta gcggtctgcc agaaacctat acaaccactt tacgaacgtt
    14521 taaaaagttt ctgccacaga tccaaaatgc gcttcactac agctattcaa acggaccatt
    14581 ggaatgctta aacaaccaca tcaaagtatt aa
//
LOCUS       DAYYBH010000063        13978 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000063 DAYYBH010000000
VERSION     DAYYBH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.065"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACHAUG_002135"
     CDS             140..460
                     /locus_tag="ACHAUG_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481217.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACHAUG_002136"
     CDS             912..1112
                     /locus_tag="ACHAUG_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8481218.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACHAUG_002137"
     CDS             1259..4048
                     /locus_tag="ACHAUG_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8481219.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACHAUG_002138"
     CDS             4095..4622
                     /locus_tag="ACHAUG_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8481220.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACHAUG_002139"
     CDS             4643..5833
                     /locus_tag="ACHAUG_002139"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8481221.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACHAUG_002140"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACHAUG_002140"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8481222.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACHAUG_002141"
     CDS             7966..8640
                     /locus_tag="ACHAUG_002141"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8481223.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACHAUG_002142"
     CDS             8637..8978
                     /locus_tag="ACHAUG_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481224.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACHAUG_002143"
     CDS             9008..9367
                     /locus_tag="ACHAUG_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8481225.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACHAUG_002144"
     CDS             9627..9749
                     /locus_tag="ACHAUG_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481226.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACHAUG_002145"
     CDS             9891..11975
                     /locus_tag="ACHAUG_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8481227.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="ACHAUG_002146"
     CDS             12286..13752
                     /locus_tag="ACHAUG_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8481228.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3939 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgttttt
//
LOCUS       DAYYBH010000064        13940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000064 DAYYBH010000000
VERSION     DAYYBH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.066"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..2271
                     /gene="acm"
                     /locus_tag="ACHAUG_002147"
     CDS             106..2271
                     /gene="acm"
                     /locus_tag="ACHAUG_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8481229.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2543..3028)
                     /locus_tag="ACHAUG_002148"
     CDS             complement(2543..3028)
                     /locus_tag="ACHAUG_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8481230.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3118..3463
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3508..4149
                     /locus_tag="ACHAUG_002149"
     CDS             3508..4149
                     /locus_tag="ACHAUG_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8481231.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4168..4803
                     /locus_tag="ACHAUG_002150"
     CDS             4168..4803
                     /locus_tag="ACHAUG_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8481232.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4821..5636
                     /locus_tag="ACHAUG_002151"
     CDS             4821..5636
                     /locus_tag="ACHAUG_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8481233.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5955..7391
                     /locus_tag="ACHAUG_002152"
     CDS             5955..7391
                     /locus_tag="ACHAUG_002152"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8481234.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7580..10027
                     /locus_tag="ACHAUG_002153"
     CDS             7580..10027
                     /locus_tag="ACHAUG_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8481235.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10257..11324
                     /gene="rlmN"
                     /locus_tag="ACHAUG_002154"
     CDS             10257..11324
                     /gene="rlmN"
                     /locus_tag="ACHAUG_002154"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8481236.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11314..11520
                     /locus_tag="ACHAUG_002155"
     CDS             11314..11520
                     /locus_tag="ACHAUG_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481237.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11517..12620)
                     /locus_tag="ACHAUG_002156"
     CDS             complement(11517..12620)
                     /locus_tag="ACHAUG_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8481238.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13173..13721
                     /gene="aac(6')"
                     /locus_tag="ACHAUG_002157"
     CDS             13173..13721
                     /gene="aac(6')"
                     /locus_tag="ACHAUG_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8481239.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4773 a         2309 c         2956 g         3902 t
ORIGIN      
        1 tttagttaaa agattggaaa tgagtggggg aggaattgaa gaaaggattt ttgagagatg
       61 atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga
      121 aatattattt tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat
      181 gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca
      241 acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa
      301 aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt
      361 gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg
      421 gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta
      481 gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat
      541 acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca
      601 gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat
      661 actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca
      721 gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt
      781 aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt
      841 ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata
      901 actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata
      961 gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat
     1021 aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt
     1081 ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag
     1141 attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga
     1201 cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa
     1261 gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta
     1321 agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct
     1381 atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc
     1441 actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca
     1501 gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat
     1561 atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa
     1621 aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca
     1681 aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac
     1741 caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc
     1801 caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa
     1861 tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt
     1921 acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct
     1981 ataccaggta gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa
     2041 attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa
     2101 caaagaagca attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa
     2161 agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg
     2221 atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta gttagaatat
     2281 aatatttttt gttaaatatg actgaaatca aaagaataaa attccgattt ataatcgaaa
     2341 aaagaagcat ttttatatat gcttcttttt tcgtagaatc aataaatatg gatttggcta
     2401 actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat
     2461 tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg
     2521 aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa
     2581 caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat
     2641 gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct
     2701 ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag
     2761 gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt
     2821 ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg
     2881 atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg
     2941 acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat
     3001 aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat
     3061 cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa
     3121 atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa
     3181 gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga
     3241 aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt
     3301 tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga
     3361 tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg
     3421 aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat
     3481 ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta
     3541 ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca
     3601 ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt
     3661 caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt
     3721 tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt
     3781 atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct
     3841 caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa
     3901 atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac
     3961 ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg
     4021 aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taggcgtggc aatcatctat
     4081 cttacattga ctggtattct aacaatcata atgaaacaag ttgaaggaaa attaaattat
     4141 taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt
     4201 ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct
     4261 atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa
     4321 agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa
     4381 atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat
     4441 atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac
     4501 accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat
     4561 ccttatcagc tttctggcgg acaaaaacag cgggtagcta ttgccagagc attggctatg
     4621 aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag
     4681 caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc
     4741 catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat
     4801 taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc
     4861 ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca
     4921 acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag
     4981 ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat
     5041 atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca
     5101 atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag
     5161 tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag
     5221 gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg
     5281 gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg
     5341 ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg
     5401 gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag
     5461 acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt
     5521 cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg
     5581 aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa
     5641 taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct
     5701 gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt
     5761 caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca
     5821 caaaattttt cttactactc gaaactaagc gactttttca caacctcctt tacataattt
     5881 tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg
     5941 aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg
     6001 gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat
     6061 cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt
     6121 tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc
     6181 tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag
     6241 tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata
     6301 ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata
     6361 atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg
     6421 aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt
     6481 cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg
     6541 aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag
     6601 aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta
     6661 atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg
     6721 atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat
     6781 gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc
     6841 ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg
     6901 ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa
     6961 tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc
     7021 cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga
     7081 ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg
     7141 actatcgaat caattactta caaagccacg tcaaagccat caaagaagca atttcggatg
     7201 gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg
     7261 gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt
     7321 tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag
     7381 agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt
     7441 cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa
     7501 gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg
     7561 atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac
     7621 gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt
     7681 tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat
     7741 tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc
     7801 cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg
     7861 ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt
     7921 tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt tgttagccag
     7981 tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct
     8041 ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt
     8101 actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat
     8161 ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg
     8221 aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg
     8281 cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt
     8341 gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga
     8401 gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga
     8461 gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt
     8521 gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga
     8581 ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga
     8641 gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt
     8701 cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc
     8761 ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt
     8821 agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga
     8881 agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa
     8941 ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac
     9001 ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc
     9061 agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa
     9121 actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct
     9181 gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca
     9241 agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta
     9301 caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt
     9361 catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga
     9421 agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc
     9481 tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag
     9541 aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc
     9601 agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat
     9661 ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac
     9721 agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac
     9781 aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga
     9841 aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc
     9901 agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa
     9961 gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga
    10021 catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat
    10081 gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg
    10141 tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa
    10201 ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg
    10261 aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg
    10321 gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta
    10381 atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta
    10441 tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt
    10501 tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct
    10561 tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg
    10621 ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg
    10681 tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg
    10741 ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg
    10801 atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac
    10861 acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg
    10921 cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga
    10981 aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat
    11041 atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat
    11101 taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac
    11161 atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga
    11221 aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg
    11281 gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt
    11341 ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag
    11401 agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag
    11461 caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa
    11521 ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga
    11581 atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt
    11641 gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt
    11701 aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac
    11761 tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat
    11821 ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta
    11881 tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact
    11941 tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa
    12001 gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct
    12061 agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata
    12121 cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt
    12181 acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca
    12241 cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta
    12301 gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac
    12361 aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt
    12421 gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt
    12481 attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca
    12541 tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt
    12601 aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt
    12661 taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat
    12721 aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa
    12781 caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc
    12841 gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa
    12901 aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc
    12961 atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg
    13021 aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat gaaagaatag
    13081 agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact
    13141 gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata
    13201 atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg
    13261 gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag
    13321 cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag
    13381 gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc
    13441 gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag
    13501 gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat
    13561 ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat
    13621 taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt
    13681 ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag
    13741 aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg aatttttgtg
    13801 aaattccaac ttatttccga agaagctact tttggaacac cgtgtaccag aggttgtgaa
    13861 aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc tacatttttg
    13921 cataaatttg gcttaatacc
//
LOCUS       DAYYBH010000065        13913 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000065 DAYYBH010000000
VERSION     DAYYBH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.067"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..229)
                     /locus_tag="ACHAUG_002158"
     CDS             complement(<1..229)
                     /locus_tag="ACHAUG_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8481240.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFS"
     gene            complement(708..1427)
                     /locus_tag="ACHAUG_002159"
     CDS             complement(708..1427)
                     /locus_tag="ACHAUG_002159"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8481241.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1476..3122)
                     /locus_tag="ACHAUG_002160"
     CDS             complement(1476..3122)
                     /locus_tag="ACHAUG_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM8481242.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3301..3891
                     /locus_tag="ACHAUG_002161"
     CDS             3301..3891
                     /locus_tag="ACHAUG_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8481243.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3983..5488
                     /locus_tag="ACHAUG_002162"
     CDS             3983..5488
                     /locus_tag="ACHAUG_002162"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8481244.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5572..6924)
                     /gene="celB"
                     /locus_tag="ACHAUG_002163"
     CDS             complement(5572..6924)
                     /gene="celB"
                     /locus_tag="ACHAUG_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8481245.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6924..7442)
                     /locus_tag="ACHAUG_002164"
     CDS             complement(6924..7442)
                     /locus_tag="ACHAUG_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481246.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7458..7784)
                     /locus_tag="ACHAUG_002165"
     CDS             complement(7458..7784)
                     /locus_tag="ACHAUG_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8481247.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7797..9776)
                     /locus_tag="ACHAUG_002166"
     CDS             complement(7797..9776)
                     /locus_tag="ACHAUG_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8481248.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9797..10111)
                     /locus_tag="ACHAUG_002167"
     CDS             complement(9797..10111)
                     /locus_tag="ACHAUG_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8481249.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10279..10572
                     /locus_tag="ACHAUG_002168"
     CDS             10279..10572
                     /locus_tag="ACHAUG_002168"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8481250.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10650..11762)
                     /locus_tag="ACHAUG_002169"
     CDS             complement(10650..11762)
                     /locus_tag="ACHAUG_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8481251.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11915..12763
                     /locus_tag="ACHAUG_002170"
                     /pseudo
     CDS             11915..12763
                     /locus_tag="ACHAUG_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13116..>13913)
                     /locus_tag="ACHAUG_002171"
     CDS             complement(13116..>13913)
                     /locus_tag="ACHAUG_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8481252.1"
                     /translation="LDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVL
                     QGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFE
                     IERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGA
                     DGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSD
                     IRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4351 a         2841 c         2378 g         4343 t
ORIGIN      
        1 aagaaaataa aaatttgaaa ttttcccaac ccacccaagg actttccctc aagctagcca
       61 agaacccatc tgctgaaata tgaaaatttt tgaatgctac gacattcgcc attacaggta
      121 tgtaaaagaa aaaaatcatc caaagcatac ccggtacagc catcaggaca agtgctttat
      181 aacgccatag attcataaaa aaatgattga acccctttcg ttttttcatc taaaaacccc
      241 tttcgattca ttcgcaacgt aattatagcg ctttcaataa tgagaataaa taaacaaagt
      301 atagggaaaa tgaaacaaag tataggagag gttgtgaaat cagcgttttg gcctgagtat
      361 tagagaaaaa aacgggaaaa tagttcctca tattttgccg ttttttgagc taataccgag
      421 ggtcagctga ttgaacaccg tttatcagag gttgtgaatt cagcgttttg gcctgagtat
      481 tagggagatc gtgtccttac tatctaactc tgagtcacaa ggaacaatgg ggactgtaac
      541 aaaagtcttg tcacagtccc ttattccgaa caaacggtgt tccagaagta gcttctacgg
      601 aaataagccg aaatttcaca aaaaagccat ccgaaagtca gacggacaag tctgcttttc
      661 agatggcgct acaactactc tgcttattct tttagctatc cattcaatta tttttctctc
      721 acttgttcta gctcttcttt ggtcatgggt cgatattgcc ctttattcaa atctttaggc
      781 agccaaaatt ttcccatacg gattcttttt aaatataaga cttcttttcc tactgctttg
      841 accattcgct taacttggtg aaactttcct tcatgcaaaa tcagacggat aattgaagtt
      901 tctgtctctt gatctacttg atcaatttgg agttttgccg gcaatgtctg ctcatcgcca
      961 tcaataatca gtccttctgc aaataaacgg atatcttctt ctgtcattat accgctaacc
     1021 tcagctagat attctttctc cacatgtttt ttaggtgata gtaactgatg ggcaaacacc
     1081 ccgtcattcg ttaaaatcaa gaggccctct gtgtctttat ctaatcggcc gactggaaaa
     1141 agatcttccc gataatcctg gtcttccaat aaatcgatta cggtttgatc atgattatcc
     1201 tgagtagcag aaatgactcc tgcaggttta tgcaaaatgt agtaaaaatc tttttgatac
     1261 gtaatgattt ctcctaaata agtaaccgca tcttcgaatt catttacttg cgctttatcg
     1321 cttttcacca cttgtccatt gacttcgatc gttccttttt tgattaatcc tttgacttct
     1381 ttgcggctgc caagtcccat atctgctaaa aacttatcta aacgcatgcg tttcccccat
     1441 ttcaaaagag catcaatcta gctcttctga ttcacttatt ttatttttaa tttatgtcgt
     1501 aaacgcgcta ttcctggtcc tagtagtctt tctgccaagc ggattttcaa agctaaataa
     1561 ccatacacta aagcccctat acctgctact acaagaatca acaatagaga ctggaatttg
     1621 ctatcttcgt ttaaaaaagc accaaaaatc atctttgtga tccacgaagc caaaagcata
     1681 atcaaggtaa gaataaagat cagcaatgtc ctgcgccaga caaaacttgg tttaaagcgg
     1741 gctacttcat ggattctctt taagatcaag gtacaagaca cagcaaatcc gatcattgtc
     1801 gccaataaag ggccgtatac ttcaaataaa cgaatggctg ggtattgaag aaggagtttc
     1861 actagaaacc caataaccaa ataattaatt gccgccttgt tttcaaacat tccttgcagc
     1921 atgttggaaa ctagcatata tagtcctagg aagagtccta caaaacttgc ttggatcagt
     1981 acattacttc ctagagaatc cggtgtataa aataatgtat ttagcggata ggcaagtaac
     2041 ataacaccaa atgtcgccgg aaacataaaa aatgaaaaca actgtaaatt tccgcttgtc
     2101 aattttgcca gacctactcg atctttcaac gtaacagctt ctgtaatcaa cggtaaccca
     2161 gtagacgcga tcgatgttgc caatgcaata actaccatcg tcaacttatc tggattggca
     2221 ctaaaaatcg aaaaaagatc cagtaactgt gcattggaat actcggtgga tgcagacatg
     2281 atcttcataa aagtgaactg atcgactaat ttgaagatcg ttacccctga acccacaata
     2341 ataaacggaa tcgcttcttt aacggtatct aacaacaatt ccttcgttgc aatatttatt
     2401 ttgttttcgc tgtagtgaat aaacgccgat gtatacgctt gatgtttctt caaaaaatac
     2461 aaaaggacaa gaatacttgc tagcataccg ataaatgccg cgaaagttga ttgcgtgacc
     2521 gccgtcacat agtcaccatc catgactttc atgatgatga atgtagccaa tagcatataa
     2581 aaaatacgtg ccacctgttc tacaatctgc gacagcgcat aaggcatcat ttcttgattg
     2641 ccttgcaaat accctcgtat cacactcata caaggaaaaa ccaatacagc gatactcaaa
     2701 gaacgcatcg ttggaatcag ttcttctccg ccaccagaag catgagctag ccaaggagag
     2761 gcgatgtaca tgaacaaagc aaacaacagg cctaacccgc ccatcatctg taatgcacgg
     2821 ataaataatc gtcgtgaagt gccatattca ttcaatgaat tatagcgtgc agtttgtttt
     2881 gcaatcgcag caggtattcc tgctgtagag atcagtagaa acaatgcata tatattgtat
     2941 cccatattga ataatccatt ggcagcctta gcattttcgc ccatccaagc ataccaggga
     3001 atgatataga cagcgcctag caatcttgaa atgatattac tggcggtcat ccaagcagag
     3061 cctcgtgcca ttttttcttg cggtgttaat tgatctaatg ctggttttct ttgattatcc
     3121 atcggtcaga ttccaacttt ctacaaaaca ttactatcat tttaattggt aaaacagaaa
     3181 ctggcaatcg attttatata acttttataa gaacaattgt ttttttgaat tcggtttcgt
     3241 ttttttggta aagtaaagaa atatacagcc ttttatggct aacatttata ggagatatca
     3301 atgacacaag aaataaaaaa actacccgtt atatttttag gtcttttctt tatcacagtt
     3361 ggattgaatt ggttcttgct ccctcacgat atagcttccg ccggagtggg cgccatcgga
     3421 catttagttg aaacggcctt ttctatccaa cgctcagtta ctgtttggag catcaatctt
     3481 atgatgctgc ttctagccgc tgtcctatta ggaaaatcag tattttttaa gactgtagcg
     3541 ggaagtctgc tttttcctgt tgttttagag attgttccaa aagtggaaat gatctcctca
     3601 catttctttt cactgatttc aggaagtctt ttgttcagtc ttggggttta cacattgtac
     3661 acagtcgggg cctcaaatgg cggtataacg ataccgccga ttattttcca caaatttttt
     3721 cggcttccaa tgccgcaagg acttttactt accaatagtc ttatcgtctt tttgaattat
     3781 tatgtttttg gattgttgga gacactattc gttctcctat ctattacagt cagttcattg
     3841 tttatgaaaa tactcatccg cttgtcccct gtgtcaagaa aatttgaata aagctggatt
     3901 ttgtcatagc ggaaacaaac gatatattaa aaagagtgtg ctataataaa ccaagaaatt
     3961 gaataaagcg agggatcaat caatgagctt ctctttagaa gacatccgtc atttactatt
     4021 aaaagaaaat ctattaaaag aatttgtttc aacaagaggc tggcatcttg atgtaccaga
     4081 aaaagctgaa tttcttcagt taagttatga ttcaagaaaa gctgatgctt ctactttgtt
     4141 tttctgtaaa ggaatgaatt tcaaagaaga atatttggat tcagcaatcg aacagggtat
     4201 tcaatattat gtatcagaac aaccatacga aaattcagca gtaggaatca tcgtaacgga
     4261 tatccgtaaa gcaatggcgc ttcttgctat ggctttttac gactatccgc aaaataaatt
     4321 gaaagtgatc ggattcactg gaacaaaagg taaaacaact gctgcgtact ttacaaaagc
     4381 aatcttggat cacacaacaa ataaaaaaac agcattgctt tctactatga atacaacgtt
     4441 agatggcaaa acatatttca aatcccattt gacaacgcct gagtcattag atttatatcg
     4501 tatgatgtca gaagcagttg aaaatggtat gactcatcta gtgatggaag tttcttcaca
     4561 agcttacaaa acacaacgcg tatacggctt gactttagat gtcggaatct tcttgaatat
     4621 ctcgcctgac catatcagtc cgatcgaaca tccgacattt gatgactatt tctattgcaa
     4681 gcgacaattg atcttgaatt caaaaacagt tgttttaaat catgaaagtg actattttga
     4741 tttacttagg gaaacagccg atctattcca gattccaacg atcacttatg gtcgttcgga
     4801 agaaagcaat tatcaagtga tccgttcaga taaaggtact cacggtttta ctttatcttc
     4861 tcatgaagat cgcttagcta tctgcgatac tgcttatgat attttgctgg ctggaggatt
     4921 caaccaagaa aatgcagcca gtgcgatcat tgccgcagca ctaacaggtg cctccagtca
     4981 agatgcacaa gacggattaa aagaagctcg tgtaccaggc cgaatggatc aattgatcca
     5041 atccaatgga gcgcatgtct atgtggacta tgcacataat tatctaagct tgaaaacatt
     5101 gctgaaattt gccaaaaatg aacatccaga tggccgtgta attgttgtat taggcagccc
     5161 aggaaacaaa gcaatctccc gtcgacatga ttttggaaaa gtgctatcag aaacagctga
     5221 catcgccttt ttgacggcag atgacccagc atttgaagat ccgaaaaaaa tcgcggaaga
     5281 aatcaatgaa gcaattacta atccagattt gatcgttcat tatgaaatgg atcgtcctga
     5341 agcgattcga cgcgctttag cagaaagcgc tccgcaagat tcagtcgtta ttgcgggaaa
     5401 aggcgtggat ccctatcaaa aaatcaatgg tgtagatgaa ccttatgaag gggattatgc
     5461 aatcgtgaag cggttgattg aagaataatg gtatttatat actagtggtt tcccatttct
     5521 aagaaaaaaa gggactgtga ttttttgtca cagttccttt tttcttagaa atcattttaa
     5581 cttttcacta gctaattctg ctgccttttc tctttcttca tttaataatt tattatcata
     5641 gaatttgata aatgggaacc agataatgaa tgcgatgatt gcacaaatga ctgctaacac
     5701 agcagctttc caatcaccac cactaccaac aaatgctccg atcccgactg gtgaaggcca
     5761 cggcatctgt gcaagcatcg gtttgacgat ttccagccga atggcaaaat aagcgagtgt
     5821 agcagaagcc atcggagcta agaaaaacgg aaccgctaaa tagggattgt agacgattgg
     5881 catcccgaaa atgattggtt cattgatatt gaataatcct ggtacaagtg aggcttttcc
     5941 taacactttc agctgatcag atttcgcgag gaatgcaata aagatgatca atcctaaagt
     6001 cgcacctgat ccaccaacgg ttacataaga attgttgaat tctcctgcca acggataatt
     6061 tgcgccttgt gcgttagctg ccatattagc taatacgatc ggggtcaaga aagaagtgat
     6121 gatattcgct ccatggatcc ctacgatcca caatgcatgg atcaagaaat aaatcaccat
     6181 gacaccaagc catgtattcg tcaactgcgt aacaaaccca aatgggatag caatcatttt
     6241 gaagatatcc gttcctagtg caactaataa tccattgatg atcatcacca caaacgcaat
     6301 gacaaatgtt gggatcagag cagtgaaaga acgagaaact cctggcggaa caacatctgg
     6361 catttttatg acccaatttc tcttgacaca catacaatac aattcgaccg caataatcga
     6421 cataataata gctgtaaaga ttcctgaagt tcctaaacgt tcgacaaaag agcccatcct
     6481 aataccattt actacagttt ctgtctcatt gatagaagca atcaagctga ttttaccatc
     6541 agtcatgatc aattcaggga tacacataaa gaatgcaaaa acagatagta atgcccccgt
     6601 gagcggatta acatttaatt tttgttcatc tcgttcaatt gacgttaatt cataagcaaa
     6661 cacaatggca aagtaaatag ataaaatccc catcgtcatt gtattgacga tcatgtacaa
     6721 gtcactaact ttgaaaaaag ttgcattaaa aaatccttct aaaaatggga acggtaatgg
     6781 caaaatattt aaaaccaaaa acatcgagcc tacaatcgtg aaaggaatag aagccatccc
     6841 cgctgccatg acagcccgaa cgattttaag ttgcgcaatt ttccccatag gtcccatcag
     6901 gtatttctgc aaaaaatcaa acattagtta ttctcctttc ctaaatataa cgctttttca
     6961 tattgtttaa tacggatata ctgttggtct tggttgagtg aaattttctt taaacgatac
     7021 tgtataaatg cactggcaaa aataccgctt acaacaagaa tgaaaacaaa taactgtcct
     7081 ccattgttat ttgccataaa aggatacaag aatgtaaatc ctgaggtaaa caacactgga
     7141 atcactaata aattaaagac actctggaac aaaaaatacc actttgtcca tggaacgttc
     7201 gtttggtgtt ttcgataaag ctttacttgt tctcctactg caggcaaaat agctaaaatc
     7261 agaatcatcg gtaaactact tcctaaactt ttagctgata gaagcagaat catccattgc
     7321 atgttcatga aaaagaaaaa ggcagtcagg taacgtacga ttagataacg attgaaatac
     7381 atgtttttca aactaagttg ctttttttct aattcgattt tcctttttac tgtatcctgc
     7441 acaatgatcc ttcctttcta tttatttttt tgatacaaat aattcatttc ctgtgcaact
     7501 tccaataatg tcatcgtcgt catcagatga tcttgtgcat ggaccatgat aatttccatt
     7561 tctatttttg tcccgctggc atattctttc agaagttgag tttgtgactg atgcgcatga
     7621 agaatctctt catttgcttg tcgcaacttc tcttctactt cttcaaacat ctctttacgc
     7681 atcgagcgaa atgcttcatg tatcagtgtc cgtgcatttc cgctatggaa aatgatatca
     7741 aaagaagtca cttgtagtgt ttcactgttt attttttcag ccatctgttt tcctccttat
     7801 tcgatcagtt tgataaattt actgctgaaa ttctcaaaaa taggttcagc taagatttcc
     7861 tgctgtactt ttagctggtc aactaaccta acgattgctt tagtcacgac cgtaattcct
     7921 tcattttcga cataagaagg cgaaactaaa aaaacaaaat gaatttcttg atattgatcc
     7981 cactgcatcc cagaaggaat caatgcaacg gctacctttg tttcgattcc aactggaaca
     8041 gcgggatgtg ggactgccac tgtatcactg aatatgattt gtcccatatt ttcacgttgc
     8101 tggatctgct ttcgcatttc tcttatatat ccttcttctt catttatgct cagcttttgc
     8161 agcaaatcat gaagaatttc ttcctttttc ggttcgtacg tataagtctt gaaaaatttt
     8221 tcagacaact gttcggtgta aatctgcttt ttttgctgta gagaaaatgc tggtatcggt
     8281 ctatctgagg aatgataggg tatagattct ccaactacct ttcgtatttt acgaacatca
     8341 tcttcattca aaaaaatact gacatgtaaa actggaattt tgaaaaacat tgtcgaaaga
     8401 tcaatagaag aaataatcag gtcgattcct tttaaagttg tttcattgat ctcataatag
     8461 ccttttacac ttaccactgt aatattttta ccgaattcac tgactacgcg attctttaat
     8521 agttgggcac tgccgtaacc cgttgcacag ataatcaatg catgtagttt atgcgcagct
     8581 ctttcttttt ccaatgccgc catgagatgc aaagccaaat aggcccattc atcttcattg
     8641 atggtatagt ttttcaaagc cggcatgttg ctgaaatgcc tttttactgc gtcaaagact
     8701 ttgggatttt ctttctttat ctcctcagtc aatgggttct caatgcgaat acctcggctc
     8761 aatcgaatca tcatcggtga caaatgatcg agtagtccat tgaaaagctg gagatcctct
     8821 actaatgaaa aatgaaattc ctgagacaat cttttcagaa ctttcgctaa ttcctttgat
     8881 agttcctgat cattttgatt ttctttgtga ttcgacttag ccatgagatg taatgtcaaa
     8941 taagcaattt cttcttcggg aaacgataca cctgcgatta cctcaatacg ttgaacgatc
     9001 cgctgcgcca cttgatactc tactcgctta gaaacttctt gttcgatgcc taattccttg
     9061 atttcaaacc cttctcggag ccgtttgatc cccaaagcca agtgtaaagt cagattctgt
     9121 atgataaaat ccgaaacttt taacttagct tctcttgttt catcgagaat gataatgact
     9181 agctcttcaa aactgatgtc tgggaaaaaa cggctttgcc ctaaatattc tttcaatgaa
     9241 tttccataat ggtttccaaa aaaagtatcc atgataaaat gccgtcgatt tctttcttcc
     9301 ccttcgatcc atgtcccttg accatgctta gaaacgattt ttagtgaata tggaatcaat
     9361 cgctccttga tttcttgcat gtcctttgtc aaactggaac gactgatata aagctcttct
     9421 gccaaatcat ccataaaaag aatggcatct tccaaaagca attggttcaa tatatacttt
     9481 tgtcgatctt cggactcata aaatccttgc ggttctctag catatccagg tactatttct
     9541 tgccgactca aaaacaagtc aaatgtcagt ctttgtaaaa tatgcagttg gtagccataa
     9601 cctggtttag caatgatctt acctccgttt ttttctacta actctttgat ccgctgcaag
     9661 taattacgga ttgtccgatc agataataac aattcagaag ctaaacgttg actggtgaca
     9721 tactccttct tgtgatcaat caaatatctc aacagcaatt tttccttctg tttcattatc
     9781 atccactcct attccatcat atgttctctt cgatcacctc tgctaacttc tggatgcctg
     9841 tagggattgg gacatacgct tggaaaggga tactaacaac aggctttcca acagattctc
     9901 ctaatttttt gaatttatcc aaaaacatcg tcgtttgcgg gctgatcaga aataaatcaa
     9961 aatcactatt cttaatcata ttatcgcctt ctgtagctga aactgcatct acgagtacct
    10021 cttctctttt ttgcttaaag aaatccgttg tctttgaggc catcatcgag gatgacattc
    10081 ctgccgcaca aataatcaat gcttttttca taaatcaatc actcctttta tttttataaa
    10141 ccaagcatat cttgtaagcg ttttctattc aaaatatata tttccgtagc attgcggaaa
    10201 aaatgtgtat catatcttga ataataattt ctggcaccgt gatatactag tgatagaatt
    10261 ttttcaggag gggatttagt gagtatcaca gtgaatattt tgtatacaag tacaaatgga
    10321 caagccagaa aatttgcaga agaaatggtc gaaagaaaca ttgtcaaagc gattcgtgca
    10381 gaagaaggaa acgagaaata tgactattac tttccactag aagatccaga atctctttta
    10441 ctgatcgacc gttggaaaga tgaagaagca atcgaaaagc atcacaaatc cgaaatgatg
    10501 gctcaaataa acgaactccg taaaaagtat aaattaaaaa tgaaagtcga acgtttcact
    10561 gatttaccgt aaaaagatac acatggaatg atagcaattt atcatccatg tgtatcttca
    10621 ctttttcctt attttttatt cgatctcatc taatgattca aaccgtgttt ttctcttatt
    10681 tttcggataa gtatttcagt caccccaata tacatgattc ctaaacatac cccaaggata
    10741 ttctcaaaaa tgcggatcgt tactgctcct ggaaccccga ataaactggc agtgattgcc
    10801 aaagcaccaa aagaattaaa aactgtttta tatctatagg tagaacaaac acctaaagct
    10861 agaccaccta atataccgac ccagttaaaa ggaataaatt ggctgatacc aataaataaa
    10921 acacatccaa tcaatgagcc aatgattcgg tctactgctc gttctttgaa accaatcgac
    10981 atcaatccat agctagatac aatagaagaa aatgcaaacc ctgcccacat caatcgctga
    11041 aaagggacat actctcctgc aacgatcaaa agactaatgc caaatgcata gatgagcatc
    11101 caaatttttt cctgcgaata catgcctttc aaaaaaatct ctttaaacaa gcttttgtct
    11161 ttgttctttt ctcgatgttt tctgtaaagg agaactgaaa accaacaaaa aaacaaaaat
    11221 aataagctac ttgtttgtgt aaagaaattc ttgttcaacg aatctttagg taacgaataa
    11281 acaataaaga gatatgaaaa gccgtacaaa gaagcattcc ccattttggg atcactagcg
    11341 gttgccatta atatgctgct tagtaaaata aaatgaatca acatgcccat aactgaccat
    11401 ttgatcagct ggacatacgg tgctactaat agtgagaaca tgacgattgc taaacctatg
    11461 atcgaatggg aaatcttata accaaagtca acaaaacgaa tactcatcaa catacagaat
    11521 aaaaccacag cgtatggttt atgcttcgat tcaaataatg ttgtaattat aatgataaaa
    11581 caaatggcaa acaatacgat gaataatgat cgtaaaaaca atgaacaaat atacatgcac
    11641 ttttcttttt tagaagctgc ttctcgtatt ttttgtttta aaataaacgg atctagttgt
    11701 agagactgat aaaaatccac ctgttgtcgt cctttctttt tacttttatt ttttacagtc
    11761 atctatgaca ggttgtaaat actatggaac atcatatggc gttgtcattt tcttgtcaac
    11821 tatttttcct attcatgttt ttataaaaaa acatatcgtt ttcttgtatg attaaatcac
    11881 cacaaaataa acaactgaga gaacgatatg ctttatgaat ggttctatca aaaaaattct
    11941 gagatcaata gacaaaaatt tgatgattac agaggtctct tacgaaacct tacagaagaa
    12001 aaaaacattg atcgttgctg cagttctctt ccctgctcca tgtgtctgta gaaattgtgg
    12061 ttctgctgtg gtagatggaa ataggaaatt aatggtggtg aaaaacaaga agaaggaaac
    12121 cattgtccac tttgaataat ataatcatat gccaatggtt atacgcctaa aaaagcagcg
    12181 ctatacctgt aaaaactgtc gaagccattg gaacgctcaa agttatttta tccgtccaag
    12241 gcactcgatt tcaaatcatg tcagacataa aatcacttct ttactgactg aaaaagtgtc
    12301 gttatttttt atttcgaaaa gttgttaggt atctttgaca accgttattc gcacattgaa
    12361 agagttgaga agctatttac caaagtcttc caaaagaatt ctgtcttgag tgttgagggt
    12421 tgtgaatttc gttctcacgt ttccgttgag gataaactga gttttatttc cgcggatggc
    12481 gagacagaaa aattaatcga gaaaatttac ctacacaaaa aattcctcga ttaacaaact
    12541 atttccttag ctgcactaat cctaaagaaa tagaattctt agttacagac atgaacgcca
    12601 cctactttca gctcaccaaa catgttctac caaatgcgaa attggtgatt gatcgattac
    12661 ttggtttttc cacgcctttc aaggaggcgt aacctttatt cacgagttgg ttgaagcttt
    12721 ccgagacaaa agacccgact tgtttttctc cttattgaca aagagccatt aaaactagag
    12781 aaaaaattac aactgatcgc tgattatttg tagaaagaaa atcaaagtat tacccaataa
    12841 acttgagtca ttaggcaaaa aacactgcga aactcaagaa tgattttcgc agtgtttttg
    12901 tatatttttt attaactagt attactacaa aggtttattt ttcacttctc aacgcagcac
    12961 aaaattgtct ttacttattt actggttggg ttcaaccttc tctactccga tactaatatt
    13021 tgtttgtata ccccttctaa aatcggtagt ttccattcaa ttgatgggta ctagttgatt
    13081 ttaaaaacta tgcttattca attttttatt ttagctcata tatttcgttt ctttctaaat
    13141 tttatactga tgaaaacaag tagcacaccc aatattgaca atataaactg gctaatgatg
    13201 gtttctccag tttttggaag atgcttattt ccactgcttg ttggattgtt tcctcttata
    13261 tctgagctat cagcaacttt tctattctct tttgttaaaa agatcgccac ttcttgttct
    13321 ttttcgatct tgaaagttac tggtgtcgca tccaattcat accccgttgg cgcttgagtt
    13381 tctaccagct gatatgctcc tggtgctaag ccatctatcg ccagttttcc atctgctcct
    13441 gtggttagac ctgactgcag aacggttcca ttggcatctt gtaactcaaa aacagctcct
    13501 tgcaggactt ctccgctttg gtcatcgatc tttgtcaaca cgacaccgcc tggtgtaagt
    13561 cgattttctt tcgtcaattc taccaccgct gtctgacttc tttcgatctc aaactcgatt
    13621 ggtgtcgcat ccaattcata ccccgttggc gcttgggttt ctaccagctg atacactcct
    13681 ggtgctaagc cgtcaatcgc cagttttcca tctgctcctg tggttagacc tgactgcaga
    13741 acggttccat tggcatcttg taactcaaaa acagctcctt gcaggacttc cccgctttgg
    13801 tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc ggtttgcttt cgtcaattct
    13861 accaccgctg tctgacttct ttcgatctca aactcgatcg gtgtcgcatc taa
//
LOCUS       DAYYBH010000066        13862 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000066 DAYYBH010000000
VERSION     DAYYBH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.068"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACHAUG_002172"
     CDS             168..362
                     /locus_tag="ACHAUG_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481253.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACHAUG_002173"
     CDS             631..930
                     /locus_tag="ACHAUG_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481254.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACHAUG_002174"
     CDS             complement(1027..1461)
                     /locus_tag="ACHAUG_002174"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8481255.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACHAUG_002175"
     CDS             complement(1693..2403)
                     /locus_tag="ACHAUG_002175"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8481256.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACHAUG_002176"
     CDS             complement(2393..3253)
                     /locus_tag="ACHAUG_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8481257.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACHAUG_002177"
     CDS             complement(3258..3896)
                     /locus_tag="ACHAUG_002177"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8481258.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACHAUG_002178"
     CDS             complement(3911..4210)
                     /locus_tag="ACHAUG_002178"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8481259.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACHAUG_002179"
     CDS             complement(4253..5737)
                     /locus_tag="ACHAUG_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8481260.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACHAUG_002180"
     CDS             complement(5757..6218)
                     /locus_tag="ACHAUG_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8481261.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACHAUG_002181"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACHAUG_002181"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8481262.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACHAUG_002182"
     CDS             7521..8291
                     /locus_tag="ACHAUG_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8481263.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACHAUG_002183"
     CDS             complement(8564..9391)
                     /locus_tag="ACHAUG_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481264.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="ACHAUG_002184"
     CDS             9959..11194
                     /locus_tag="ACHAUG_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8481265.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="ACHAUG_002185"
     CDS             complement(12193..12453)
                     /locus_tag="ACHAUG_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8481266.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="ACHAUG_002186"
     CDS             complement(12480..13781)
                     /locus_tag="ACHAUG_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8481267.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4463 a         2834 c         2179 g         4386 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagacccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 ta
//
LOCUS       DAYYBH010000067        13807 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000067 DAYYBH010000000
VERSION     DAYYBH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.069"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHAUG_002187"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHAUG_002187"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8481268.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACHAUG_002188"
     CDS             complement(2260..2511)
                     /locus_tag="ACHAUG_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8481269.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACHAUG_002189"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACHAUG_002189"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8481270.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACHAUG_002190"
     CDS             3461..3679
                     /locus_tag="ACHAUG_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481271.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="ACHAUG_002191"
     CDS             complement(3968..4480)
                     /locus_tag="ACHAUG_002191"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8481272.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACHAUG_002192"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACHAUG_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8481273.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="ACHAUG_002193"
     CDS             complement(6933..7376)
                     /locus_tag="ACHAUG_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8481274.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="ACHAUG_002194"
     CDS             complement(7377..8165)
                     /locus_tag="ACHAUG_002194"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8481275.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACHAUG_002195"
     CDS             complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACHAUG_002195"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8481276.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="ACHAUG_002196"
     CDS             complement(9280..9867)
                     /locus_tag="ACHAUG_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8481277.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACHAUG_002197"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACHAUG_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8481278.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="ACHAUG_002198"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="ACHAUG_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8481279.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="ACHAUG_002199"
     CDS             complement(12784..13719)
                     /locus_tag="ACHAUG_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8481280.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DAYYBH010000068        13647 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000068 DAYYBH010000000
VERSION     DAYYBH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.070"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..265
                     /gene="yaaA"
                     /locus_tag="ACHAUG_002200"
     CDS             23..265
                     /gene="yaaA"
                     /locus_tag="ACHAUG_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8481281.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            252..1376
                     /gene="recF"
                     /locus_tag="ACHAUG_002201"
     CDS             252..1376
                     /gene="recF"
                     /locus_tag="ACHAUG_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8481282.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1373..3319
                     /gene="gyrB"
                     /locus_tag="ACHAUG_002202"
     CDS             1373..3319
                     /gene="gyrB"
                     /locus_tag="ACHAUG_002202"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8481283.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3339..5810
                     /gene="gyrA"
                     /locus_tag="ACHAUG_002203"
     CDS             3339..5810
                     /gene="gyrA"
                     /locus_tag="ACHAUG_002203"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8481284.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6046..6345
                     /gene="rpsF"
                     /locus_tag="ACHAUG_002204"
     CDS             6046..6345
                     /gene="rpsF"
                     /locus_tag="ACHAUG_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8481285.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6395..6907
                     /gene="ssb"
                     /locus_tag="ACHAUG_002205"
     CDS             6395..6907
                     /gene="ssb"
                     /locus_tag="ACHAUG_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8481286.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6933..7169
                     /gene="rpsR"
                     /locus_tag="ACHAUG_002206"
     CDS             6933..7169
                     /gene="rpsR"
                     /locus_tag="ACHAUG_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8481287.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7312..9285
                     /locus_tag="ACHAUG_002207"
     CDS             7312..9285
                     /locus_tag="ACHAUG_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8481288.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9291..9743
                     /gene="rplI"
                     /locus_tag="ACHAUG_002208"
     CDS             9291..9743
                     /gene="rplI"
                     /locus_tag="ACHAUG_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8481289.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10017..11384
                     /gene="dnaB"
                     /locus_tag="ACHAUG_002209"
     CDS             10017..11384
                     /gene="dnaB"
                     /locus_tag="ACHAUG_002209"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8481290.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12164..13456
                     /locus_tag="ACHAUG_002210"
     CDS             12164..13456
                     /locus_tag="ACHAUG_002210"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8481291.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4540 a         2471 c         3009 g         3627 t
ORIGIN      
        1 taatgaaaat gagggatggg cattgaaaca acaagtcatt ctaaatacgg aattcatgac
       61 gttgggccaa atgttgaaag aagtcacagt tatcggtagt ggcggacaag caaaatggta
      121 tttggcagaa aacactgtgt tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact
      181 ttatcccggc atgatggttg aagttccaga agtaggaact ttttttatgg taaaaaaagg
      241 aaacgacgat aatgaggctg aataagctgt atttaaaaaa ttatcgaaat tacgaagagc
      301 tgaacatcga attttccaag aacctcgtca tctttttagg agaaaatgcg caaggaaaaa
      361 cgaatctttt ggaaagtatc tacgtattag ctatgacgcg aagtcatcgg acaagtagtg
      421 agcaggaatt gattggctgg actgacgatc aggcgatgat tcaaggagag atcacaaaag
      481 gttcatccac tcttccatta gaaatcttat tatctaaaaa aggccgaaaa accaagatca
      541 accatatcga acaaaaaaag ttaagtactt acgtgggaca aatgaatgtc attttatttg
      601 caccagaaga tctttcttta gtaaaaggca gcccgcagat caggcggaag tttttagata
      661 tggaattagg ccagatcaat ccaatctatc tttatgatct tgtccaatac caagcaattt
      721 tgaaacaaag aaaccagtat ttaaagcaat tgaatgaaaa aaaacaaact gatttgcttt
      781 atttggatat tttgtccgaa caactagcag cgtttggcag caaagtattg aaagcaagag
      841 cacagttcgt caagcgattg gagttttggg ctaattcttt gcatcagcaa atcacccatc
      901 aaaaagagca gctggaaatc gagtatctca ccgcagtcga ttctttagaa acacatacgc
      961 aagagcaaat ccaagaacaa tttctcgcac ttttgaacca aaataaaaag aaagaccttt
     1021 ttcggggaac aacgacagtt ggtcctcatc gtgacgatct ttcctttttt attaatcaga
     1081 aaaatgtgca gacttatgga tcgcaaggac agcaaagaac aactgcacta agtgtcaaat
     1141 tagcagaaat cgatttgatt aaagaagaga cgggggaata tccaatcctt ttgttagatg
     1201 atgtgatgag cgagctggat gatagtcgtc aactccatct gctagaaaca atcgaaggaa
     1261 aagttcaaac gttcttaaca acaacgacac ttgaccatgt aaaaaataaa atgactgttg
     1321 aacctgagat tttttatgtt caacaaggga aactaaaagg agaagaaact gaatgacaga
     1381 agaaagaagt ttagtagaac gcgctaaaga gtatgatgcc agtcagattc aagtacttga
     1441 aggacttgaa gccgttcgta aacgacctgg catgtatatc ggttcgacta gttctgaggg
     1501 tctgcatcac cttgtttggg aaattgtcga taattcgatc gatgaagtat tagcaggttt
     1561 tgcaacgaaa atccatgtga ttattgaaaa agacaacagt atcaccgtta ttgacgacgg
     1621 tcgggggata cctgtagata tccaggcaaa aactggacgt cctgcagttg aaacggtctt
     1681 tactgtttta catgctggag gaaaattcgg cggtggtggg tacaaagttt ctggtggact
     1741 tcacggagta ggatcttctg ttgttaacgc gctttctaca cagcttgatg tcaaagtata
     1801 caaagaagga aatgtgtact atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa
     1861 gattatcgaa caaacggatc gccatggaac aacggttcac tttacgccag accctgaaat
     1921 ttttacagag acaactgagt ttgatttttc aaaattggct acccggatcc gtgaacttgc
     1981 tttcttgaac cgcgggatgc gtatatcgat tgaagacaaa cgagaagaag aaccagtcat
     2041 caatgaatac cattatgacg gcgggattaa aagttatgtc gaatacttga atgcaaacaa
     2101 aaccgtgatt tttccagaac ctgtatattt ggaaggtgaa cagcaagata tcgcagttga
     2161 agtctctatg caatatacag acggctacca ttccaatatc atgagttttg ccaacaatat
     2221 ccatacgtat gaaggcggaa cacatgaatc aggattcaag actgctttaa ctcgtgtgat
     2281 aaatgattat gcccgaaaac aaaagctgat gaaagaaaat gacgacaact tgaccggtga
     2341 agacgtgcga gaaggattga cagctgttat ttctatcaag catcctgacc ctcaattcga
     2401 aggacaaaca aaaacaaaat tgggcaattc cgaagtacgg acagtcactg atcggttatt
     2461 tagcgaacat ttcatgaaat tcttgctgga aaacccaagt gtgggccgtc aaattgttga
     2521 aaaaggatta ctagcttcca gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag
     2581 acgtaaaggt gctttagaga ttagtaatct gccaggaaaa ttagcggact gctctagcaa
     2641 cgatcctgaa aaatgcgaat tgttcatcgt cgaaggagat tcggccggcg gatcagctaa
     2701 gcaaggtcgt aatcgagagt ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt
     2761 tgaaaaagca agcatggaca agatcttggc aaatgaagag atccggtctt tatttactgc
     2821 tatgggtaca gggtttggag aagattttga tgtatcgaaa gctcgctacc acaaattagt
     2881 tatcatgact gatgccgatg tcgacggcgc gcatatccgt acattgctgc tgactctttt
     2941 ttatcgttac atgcgtccta ttgttgaagc tggatacgtc tatattgctc agccaccttt
     3001 atacggggta aaacaaggga agaatattac gtatgtacaa ccaggaaaaa atgcagaaga
     3061 agagttgaaa caagtcgttg cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta
     3121 taaaggactt ggagaaatgg atgatcatca actatgggaa acaactatgg acccaagcaa
     3181 tcggatgatg gcccgcgtca gtgtggacga tgctattgca gcagatcaga tatttgaaat
     3241 gttgatgggg gatcgagtag aaccccgtcg tgcattcatc gaggaaaatg cacactatgt
     3301 gaagaactta gatatttaat catgaaggag gaaaaagaat gagtgaagaa atcagagaaa
     3361 acatccatga tgtcaatcta accagcgaaa tgaaagactc cttcatcgat tatgcgatga
     3421 gtgttattgt cgcacgtgca cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc
     3481 gtattttata cggcatgaac gaattaggtg taacaccgga taaagcccat aaaaaatctg
     3541 ccagaatcgt cggagatgtc atgggtaaat accatcctca tggagactat gcgatctatg
     3601 agtcaatggt acggatggca cagccattca gctaccgtta tatgctagtg gatggtcatg
     3661 gaaactttgg ttctgttgac ggtgacggtg ccgccgctat gcgttatacc gaagcacgga
     3721 tgagcaaaat cgctacagaa atgctgcgtg atatcaataa gaacactgtt gatttccaaa
     3781 gcaactatga tgatacagaa aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt
     3841 tagtgaacgg aacaacgggg atcgccgtag gtatggccac gaatatccca cctcataact
     3901 tgtctgaggt agtagcggct atcgatttgc tgatggataa tcctgatgtg acgaccaatg
     3961 aattgatgga agtattacct ggaccagatt ttccaacagg tggtttggtt atgggaaaat
     4021 caggaatccg aagagcgtat gaaacaggta agggttctat taccgtacgt gcaaaagttg
     4081 agattactga gatgccaaat ggtaaagaac ggatcatcgt gacagaattg ccttatatgg
     4141 tcaataaagc aaaattgatc gaacggattt ctgaactgca cagagacaag cgtatcgaag
     4201 ggatcacaga tttgcgggac gaatcttccc gtgaaggtat gcggatcgtc attgacatcc
     4261 gtcgtgacgc cagcgcatct gttatcttga ataacttata taaattgaca tccttgcaaa
     4321 attcatttgg attcaatatg ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga
     4381 aacaaattct tgaaaactat atcgaacatc agcgcgaagt catcacaaga cggacagtct
     4441 ttgaaaaaga aaaagcagaa gcaagggcac acattttaga aggtttgcgt attgctttgg
     4501 atcatatcga tgaaatcatt gcgattatcc gcgggtctaa atcagatgac gaagcaaaag
     4561 cgacgatgat cgaacggttc gatttatccg atcgccagtc tcaagctatt ttagatatgc
     4621 gtttacgccg attgaccggt ttggaacggg aaaaaatcga aaatgaatac caagaactct
     4681 taaaactgat tgaagattta actgacattc tggcaagacc agaacgtgtt acagaaatca
     4741 tcaagaatga attagcagac ttgaatcaac gatttggtga taaacgacgc acagaattac
     4801 tagtcggtga ggtcttaagt ctggaagatg aagatttaat cgaagaagaa gaaatcgtta
     4861 ttacattgac aaacaatggt tacatcaaac gtttggcaaa taatgaattc agagctcaac
     4921 gaaggggtgg acgtggtgtc caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc
     4981 tggtttcttg ttctacccat gatacattgt tattctttac gaataacgga aaagtgtatc
     5041 gggcaaaagg atacgaaatt ccagaatacg ggcgcacagc caaaggaata ccaattatca
     5101 atttactagg tatcgattcg agtgaaagca tccaggcaat cattgcggtg acgggagaag
     5161 cagaagaagg ccactatctc ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg
     5221 tcactgcttt tgctaacatc cgaagtaatg gtctgatcgc aattggtttg aaagaagacg
     5281 atgagctcgt caatgtgcta ctgacagatg gtcaatctaa tatcattatt ggaacacata
     5341 acggctactc tgtcactttc aaagaagaag cagtccgcga tatgggacgt acagcttccg
     5401 gggttagagg aatacgcctg cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg
     5461 aagatcaaga agtattagtg atcacagaaa aaggttacgg aaaacggaca aaagtatcgg
     5521 aatatccagt gaaaggccgc ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa
     5581 atggtccact agctggattg accactgttt ctggagatga agacatcatg ctgattacag
     5641 ataaaggtgt cattatccgt ttcaacgtat cgactgtttc tcaaactgga cgttcaacat
     5701 taggcgtacg tttgatgaag atggaagctg atacgaaagt tgtcacgatg gcagcagtcg
     5761 aaccagaagc tgcagaacaa acagttgaag aacaaggatc agaagaatag ccatcaagac
     5821 taattgtgta agctagcaag aatgaccgat taaaaaatct ggttattcct gctggctttt
     5881 ttgattagtt gaatttctct taaaatattc gaaactgttc attctgtatt gcttttgacc
     5941 aaaatagagt gtataatcag agcttgtgag tagtctaatt aattagataa ctctccttgc
     6001 tcttaggaac ctaagagccg aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa
     6061 tatgaaatca tgtacattat tcgtccaaac attgatgaag aagcaaaaac tgctttaatc
     6121 gaacgtttcg atgcaatctt gaaagataac ggagcagaag ttatcgaatc aaaagattgg
     6181 gaaaaacgcc gtttcgctta tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt
     6241 aaagtatctt ctccatcatc agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc
     6301 aacgacgata tcattcgtca catgattgtt aaagaagaag cttaattgtt gtttcatgtg
     6361 aaacaataga aaacgatgag aggagatgac tattttgatt aataatgttg tattagttgg
     6421 acgtctgacg aaagatccag atttgcgtta cacagcaagt ggtacggcag tcgcaacatt
     6481 tactttggct gtgaatcgta attttacaaa ccaaagcggc aatcgcgagg ctgattttat
     6541 caactgtgtg atttggcgca aatcagcaga aactttagca aactatgctc gtaaaggaac
     6601 acttttaggt gttactggac gtatccaaac tcgttcttat gataatcagc aaggacaacg
     6661 tgtatacgtg acagaagtag tagctgacaa cttccaattg ctggaaagtc gttctgcatc
     6721 tgaaaatcgc caacaaagcg gcggattcca aagttctggt caatcagctg gcggatttgg
     6781 cggaaacaat aactctaatc aaacatctca atcatccaac ggtatgcctg atttcgaccg
     6841 tgatacttct gacccattcg gctcaagttc aacgatcgac atttcagacg atgatttacc
     6901 attctaaacg aatttgatag gagggaaata gaatggcaca acaaagaaga ggcggacgta
     6961 aacgtcgtaa agtagactac attgctgcta accatattga atatatcgat tataaagata
     7021 tcgaattact aaaaagattt atttcagaac gcggtaaaat cttgccacgt cgtgtaactg
     7081 gtacaggcgc taaaaaccaa cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg
     7141 gcttgctacc tttcgttagc gaagactaat aaatcctagg aattgaatga ctgatccttt
     7201 tgatcagcta ttcaattcct ttttttctgt aaaaataacc tctattcgtt tagtcgctcg
     7261 aatgacgaaa gttatggtaa aatcaaatta gtcatatatg ggaggaaaat tatggaaaat
     7321 aaaaacaatc atttatcccc gaaacctctc ttccttatcc tgtttatatt aatcgaagta
     7381 ttggtagtct taattttacc atttcgtttt gttagcgctt cggtcattct attgatgaat
     7441 ttatggggtg ttcgctattg gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa
     7501 aaaatccagc aggcttcgac tgtagctgaa accaatctag cttatgtctc agaagtcatg
     7561 cctgtaggag tgattgctta tcagccatca agccaaaaaa tcgaatggct caatccatta
     7621 gcattgaaaa tcaaagatca aagtggatta acagaggaag aattgctaga tacttttttt
     7681 actgcaaaag aaaaaaagaa aaaccaagtc tatctagcag ataaaacatt tcagtttgat
     7741 atggatacag taaagggtgt tgtttatttt aatgatatta cccaaaacca gttgttaaaa
     7801 ctccatcaga cgaacagtca gccggtgatt ggtattttat ctattgataa ctatgcagat
     7861 gccattgata aattggatga caaagagatt tcttatttga actctttcgt cactacacta
     7921 gtttctgact ggatgaatga ataccatgtc tttttcaaaa gaatcaacgc agaacggttt
     7981 ttcttcgtag cacagatgat cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg
     8041 gatcgctttc gagcagaagc tgaaaaacag agctcgccta tcacccttag tatggggatt
     8101 gcttatggct caagaaccct cgatgaaatc ggtggagaag ctcagaataa tttagatgtt
     8161 gctttagtcc gtggaggcga ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct
     8221 gtttattatg gggggaaatc tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg
     8281 atgagtaccg ctttgcgtcg gatttttgat gaaacaggcg atgtgtttat catggggcac
     8341 aagtttccgg atatggatgc tttaggctct gcttttggtg tcagttgtct ggcacgcttc
     8401 aaccagaaaa aagcttacat cgttatcaac gagaacgaga tcattccaga tgtagaacgg
     8461 tgtcttgaag aaatccataa gcatcctgaa ttagaaatgc aactaatctc tccagaacgt
     8521 gccatagagc tgaaaaaaga agaggacctc ctcgtgatgg tggattacca taaaccttct
     8581 ctatcgatct ctcagggcct atatgagcaa tttgataaga ttgtgatcat tgatcaccat
     8641 cgtcgtgggg aagagtttcc aagtaaacct ctgctgactt atatcgaatc ttcagcttct
     8701 tcagcttcag agctagtttc tgagctgatc caataccaaa gcagcaaaac aaatcgctta
     8761 gatcgttttg aggccacatt attgcttgca gggatggttg tagatacaaa tagtttcaat
     8821 gtccgtacaa ctgcacgaac ttttgacgtt gccagctatt taaggtcttg tggagcagac
     8881 gcatccttag tacaatattt attgagctcg gatttagcga catacttgga gatcagcaat
     8941 ttgattgctg gaagcgacta tgtgacttct gatattgttg tggcggcagg gacagacgaa
     9001 aaagagtata atagtgttat agcagcaaaa acagcagata cattgctttc gatgatcaat
     9061 atcaatgcag ctttcgtcat tacaaagcgg acagacggct tgattgggat cagtgccaga
     9121 agtaccggca cgatcaacgt ccaaatcatc atggaatcct taggtggcgg aggacacttc
     9181 acaaacgcag ctactcaact ggaaaatacg acagttgagg aaacaaaaca gaagctattt
     9241 gctgcaatca aagaaaatat gaatcaaata tatgataagg agtaatccaa atgaaagtaa
     9301 tttttttaca agatgtaaaa ggaaaaggta aaaaagggga catcaaagaa gttccagcag
     9361 gttatgcaca aaattatcta atcaaaaata acttggcaaa agaagcaacg aaaagcagta
     9421 tcagtcagtt agcaggacaa aagaaagcac aagaaaaaca cgaagctgaa atcttggaag
     9481 aagctaaaaa gttaaaagaa ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga
     9541 aagcagggga agacagccgt ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt
     9601 taaacaaaca atacaaaatc aaattagata aacgaaaaat cgaattagaa aatccgatcc
     9661 gttcactagg ctacacaaac gtacctgtta agcttcatca tgaggtgacg gctacgatca
     9721 aagttcatgt cgtacaagaa taacaaatct tgatagcgat gttcagctgg taagtataac
     9781 cagaaggaaa aaagacaaac cgaaatttat ctgagaaaac agctgcagca atgaaagcaa
     9841 aagctgcaaa tcgatttcag attgatttcg gtttcttttt tgggaacagc aaaatagcat
     9901 aaagaaaacg agtataataa aagagaacta tttttttatt acactttcgg tacgaatgat
     9961 gacttttctc tcattttctt gtaaaatgaa gcaattgaag acaaggagcg atacgtatga
    10021 atgaagtgtg gcaggatcga atcccaccgc aaaatattga agctgaacaa gcggttttag
    10081 gctctgtttt tcttgacgca gaaacgatca ttgatgccat ggagtacata gagccaaaag
    10141 acttttatcg tcgtggccat caaattattt ttgagacaat gatcgagtta aatgatcgta
    10201 atgaagcgat tgacgtggtt actgtcaaag accggctgga acaagccaat ttgttagaag
    10261 atgccggtgg tttaagttat ctctcagact tggcattagc agttccgaca gcagcaaata
    10321 tcgtctacta tgcgaaaatc gttgaagaaa aatcattgct gcgtacattg atccagacag
    10381 caacaggaat cgttacgaaa ggattcgagc aaggagaaga agttcaaaca attttagatg
    10441 atgccgaacg cagtatcttg gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta
    10501 ttgctgaagt actgaactcc acgattgaaa atatcgatcg actagctcaa aacaatgaag
    10561 aaatcacagg tctaccaact ggctatcaag gtctggataa aatgacagca ggtttccagc
    10621 ccgaagaact aatcattctt gctgcacgtc cagcggtagg taaaacagcg tttgcattga
    10681 atattgctca aaatgtagga acaaaaacag atcgttctgt tgcgatattt agtttggaaa
    10741 tgggggcaga atctcttgta aatcggatgc tttgtgcaga aggttcgatt gaggccagcc
    10801 atttacgtac aggtcagctt tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga
    10861 gtctttctcg agcaaatatt tttatcgatg atacaccagg gatcaaaatc tctgaaatcc
    10921 gtgcgaaatg tcggaaacta gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt
    10981 atttgcagtt gattgaaggt aacggtaaag agaaccgtca acaagaagta tcagatatct
    11041 ctcgccaact gaaaaaactg gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt
    11101 tatctcgtgg agtagaacag cgacaagaca aacgaccagt gctaagtgat attcgtgaat
    11161 ccggatcaat tgaacaagat gcggacatcg tagccttctt gtatcgagat gattattatg
    11221 aaagagaagg cggagatgac gatgatggaa acgagccaaa aaatgacaac gtcatcgaag
    11281 tcatcatcga gaagaaccga agcggtgcac gaggaacagt tgaactactg tttatcaaag
    11341 aatacaataa gttttcgtcg ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc
    11401 cttgagcaat cccgagagct ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag
    11461 agtacttaat ttaaacagat ctccaaaggg attgtgacaa aagtcttgtc acagtccctt
    11521 tttccgaata aacggtgttc aatcagctga cccttggtat tagctcagca aacgtcaaaa
    11581 tatgaggaac tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatcttacg
    11641 acctcttttc cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcat
    11701 caaacatgag aagcagtttt ttgaaattgc tccttgtgac tcagagttaa acggcaggct
    11761 cacaacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa
    11821 acgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg caggacagct
    11881 ttttcacaac ctctgattca cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa
    11941 tttcacaaaa atttgagaag caattttcgt acattccttc ttatttcttg aagctgacca
    12001 cttctgttac aacctctttt ttaataacca ttctaaaaag ataaagttcg ttttttaagc
    12061 gtttttataa aataaaaata aaaatcattc gattttattt gaaatatgta tctattattg
    12121 atacaatggt tcaggattaa ataatataga acgaggtgtt cacatgtcat ctgtagtagt
    12181 agtaggtacg caatggggcg atgaagggaa aggaaaaatc acagactttc tcagtgaaaa
    12241 tgctgaagtg attgcacggt accaaggtgg cgacaatgca gggcacacaa ttaagtttaa
    12301 cggtgtgact tataaactgc atttgattcc ttctgggatt ttttataaag acaagatcag
    12361 tgtgatcgga aacggcgttg tagtcaatcc taaatcattg gttaaagaat tagcttattt
    12421 acatgaaaaa ggtgtagaaa caagtaattt gcgtatttct gaccgcgcgc acgtaatctt
    12481 gccttatcat atcaaattgg accaattaca agaagatgct aagggcgaaa ataagattgg
    12541 tacaacgatt aaaggaatcg gacctgccta tatggataaa gcagctcgtg taggtatccg
    12601 tattgcagac ttattagata aagaaatttt tgaagaacgt cttcgtttga atctagatga
    12661 caaaaaccgt caatttgtaa aaatgtttga ttcagaacca cttgattttg acgatatttt
    12721 tgaagaatat tatgaatacg gacaacaaat caaacaatac gtcacagata cttccgttat
    12781 cttgaatgat gctttggatg caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat
    12841 gctggatatc gaccagggaa cttatccttt tgttacttct tctaacccag tagccggtgg
    12901 ggtaacaatc ggaagcggtg tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa
    12961 agcctatacg tcacgtgtcg gtgatggacc atttccaact gaattatttg atgaaacagg
    13021 agaacaaatc cgcgaagtag gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt
    13081 aggctggttt gattctgtag ttatgcgcca ttctaaacga gtttcaggta ttaccaacct
    13141 atctttaaac tcgatcgatg tattaagtgg attggaaaca gtaaaaattt gtacggctta
    13201 tgaattagat ggcgaactaa tctaccacta tccagcaagc ttgaaagagt tgaaccgctg
    13261 caaaccaatc tatgaagaat tgccaggctg gtcagaagac attacaggat gcaaaacact
    13321 tgcagaactg ccagaaaacg cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt
    13381 acgtatctca acattttctg tcggacctga tagaacacaa acaaacatct tagaaagcgt
    13441 ctgggcgcaa atctaaggat aaaaacgacg actgaatcag gacgttctta ccagagcgag
    13501 tcgaagtgtt tatctatacg ataataataa cttgaagagg tagtgaaaga gacgtttagc
    13561 cttattgccg ataggctgtc tcttgaacac tgtttattca gaataaggga ctgtgacaaa
    13621 aaccgtgtca cagtcccttt ttccgaa
//
LOCUS       DAYYBH010000069        13171 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000069 DAYYBH010000000
VERSION     DAYYBH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.072"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(190..804)
                     /locus_tag="ACHAUG_002211"
     CDS             complement(190..804)
                     /locus_tag="ACHAUG_002211"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8481292.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(951..1634)
                     /locus_tag="ACHAUG_002212"
     CDS             complement(951..1634)
                     /locus_tag="ACHAUG_002212"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8481293.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1743..2201)
                     /locus_tag="ACHAUG_002213"
     CDS             complement(1743..2201)
                     /locus_tag="ACHAUG_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8481294.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2285..4048)
                     /locus_tag="ACHAUG_002214"
     CDS             complement(2285..4048)
                     /locus_tag="ACHAUG_002214"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8481295.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4269..5729)
                     /locus_tag="ACHAUG_002215"
     CDS             complement(4269..5729)
                     /locus_tag="ACHAUG_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481296.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5937..6863)
                     /gene="glsA"
                     /locus_tag="ACHAUG_002216"
     CDS             complement(5937..6863)
                     /gene="glsA"
                     /locus_tag="ACHAUG_002216"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8481297.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6886..8313)
                     /locus_tag="ACHAUG_002217"
     CDS             complement(6886..8313)
                     /locus_tag="ACHAUG_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8481298.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8344..9558)
                     /locus_tag="ACHAUG_002218"
     CDS             complement(8344..9558)
                     /locus_tag="ACHAUG_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8481299.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            9908..10987
                     /locus_tag="ACHAUG_002219"
     CDS             9908..10987
                     /locus_tag="ACHAUG_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8481300.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(11288..11530)
                     /locus_tag="ACHAUG_002220"
     CDS             complement(11288..11530)
                     /locus_tag="ACHAUG_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8481301.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(11562..12119)
                     /locus_tag="ACHAUG_002221"
     CDS             complement(11562..12119)
                     /locus_tag="ACHAUG_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8481302.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(12132..12320)
                     /locus_tag="ACHAUG_002222"
     CDS             complement(12132..12320)
                     /locus_tag="ACHAUG_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8481303.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(12333..12899)
                     /gene="amaP"
                     /locus_tag="ACHAUG_002223"
     CDS             complement(12333..12899)
                     /gene="amaP"
                     /locus_tag="ACHAUG_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8481304.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3834 a         2805 c         2309 g         4223 t
ORIGIN      
        1 cgtttgatct ggcatttcta tttccttacc atctaaagta acagtagtaa cgacaaaatg
       61 gtcttgcaat ggcggaactt ctaagtactg cccttgcaga aaatcattca ctgtttcata
      121 ttcttttttc atttcttttg aatctgtcga tggtttatag cttattttta acataactag
      181 tgctcctttt tattcgggac gtgcaatcgt gaaaatttct cctattgctg cataatcatt
      241 tcctgccaaa cggcttacag cattcaattt tcttggatcg atccttcctg tcttatcatc
      301 atagactgtt tcatttaaat gatatttctg tacttctagc aaaaataaat cagctgttac
      361 tttattttta ttccgtatct cgacatgttc atataaggat gtttcaaaac gaacttttgc
      421 ttctgtcaat gctggtactg aaatgattac tgaatcagtt aaagtcaagt cagtcaggta
      481 cagttcgctt tcctcattag gcagattggc tgctgtttta tttacttctt ctacgttgtc
      541 ctcatcaagg atatggataa cagcttctct tgtctcaaga atattctttg ctgtgtcttt
      601 tactgcatct tgttttctct ggatcgccaa ggataagatg ggtcgattcg aactgacgat
      661 attgaagtaa ctgaagggtg cgatattcac tatatctttt gctgatttag tcgttactac
      721 tgctactggt ctagggatga ctgaaccaat cagcaacttg taattttcac gttctgtcaa
      781 ttctgaagga ctccgttcaa acatggtttc gccttctttc tataaaattc gttaaaaagc
      841 agatcttgaa gacaaaatga aaagtaatgc ttttttcatc ttcttagaat ctgctttaaa
      901 ctatctatca atttttcttt tttgtcaaga atctgtgccg tgcgtgtttt ttagttattt
      961 gctttacttg cggcttcgac atatctttta gtgatcaatt gcggacgggt gataatagat
     1021 ccaactacga ctgaaaaagc acctaattca tatgctttct ctacttgttc aggagagtca
     1081 gtatgtcctt ccataattac tgggatatcg acttgttcta tgatttgatt caataaatca
     1141 aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc ctgctaaagt cgtagacaaa
     1201 agatcaaaac catcttcttg cgcttgaagc gcttcctcaa aagtcgaaat atctgccata
     1261 gctaaccgac cgtgttcgtg aatcatttgg acaagttcag cagttgtttt ttctgtcgga
     1321 cgtttacgag aagttgcatc cagtgcaatg atttctgctt gcgtctccaa aagttcttcc
     1381 acttcttttt ctgtcgcagt aataaacact tctgacccat cgtaatctct tttgataatc
     1441 cctattaccg gtaaatcaac gattcgcttg atttcattga tatcttctgt cgtatttgct
     1501 cgaataccaa ctgctccgcc ttcttttgca gccaaagcca ttctccccat aataaaagaa
     1561 ctgtgtagcg gctcgttttc caaagcttga caagaaacga cgactccgcc tttgatttgt
     1621 tccagtgtat ccacaatatc tcctccagta atgttatata aaaatacagc ttcatataaa
     1681 aatgtaaccc ttttctattc aggagacaat aatgagctat ttctttcagc agacctttct
     1741 tactaaaaga aaattacttc aaattcttcg tatacgacca gcttctcttc tgtaaattct
     1801 atgttcaacg cttctttata agcatgttta aaaaactcag catggacttt ccgccaataa
     1861 gctaatgtcc gatccccttc tccttctttc caagcatgct ctttcgtgac ttcactaaaa
     1921 ggcaagatgg tcacatttat atttttgatg atacaaactg gttcattttt tccattcagg
     1981 accacgtcta attgaccaac ttttggtaaa ggctcattct cttgtttata cagatcgtaa
     2041 ccacttgatg taccagtctt tatctttttt acaactagtt cgctcaactc gtctgccatt
     2101 ttttctgtat ccccaaatga aaagaaacct aatttttctg attgataact aggatatttt
     2161 tcttggaact ttttgacata ttcttctact tgctgataca taacatccac ccctttgctt
     2221 tttataatga aaaaaccatc cagatactag tataaacaat tatctggatg gtgagcataa
     2281 tgaattattg aatattcaag acacgcagac tatcttcaac gatttgtttt ggtgttaaat
     2341 gataacgttc ataaagctgc cctacaggaa cgctatccgt gaattctttc tctgcaccaa
     2401 aatttaatac gttcattgaa gaatcaccat aataacgact gatattttcg ccaaatcctc
     2461 ctgacaaact accatcttct aatgtgatga ctaattcgtg tgtttctttt aatttatcta
     2521 atgtttctac gtcattttct gtgatcgttc ctggattgat caacgttgct tcgattcctt
     2581 tttttctaaa agcttttact gtttcttttc ctaattcaag aaaaccgcct aaagctaaaa
     2641 ttgctacttt tcctccatta tttactgttt gataggctgg aatactataa tcatttgcga
     2701 tagctgtacc atgttctaca ccatgtgtag gaattcggat tgctacaggc tgcgctgttt
     2761 gttcgaacgc ccaatctagc atagagatca tatcctcttt tgttgctgga gcaagatatt
     2821 tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt tccttggtgt gttcggtcac
     2881 tagctgagat cgttccgcca ccgactaaaa taatcactgg attctcgttg atcgctaaat
     2941 catgtgataa ttgatcatat gcccgttgca agaaagtact tgattggaag acgactggac
     3001 gtactccctg agaagccaaa gcagaagcga aagtgataga gtgctgttca gcgatcccca
     3061 cgtcaaaata atgatctgga tgtttttctt ggaatgtttt cagtccaaag gcgcctggta
     3121 tcgctgcatt gactgcggca ataggagctg tacttgtttg cattttctta tccaaataat
     3181 ccatgatgat catattaaat gtttctccag aagttacttg ctttgcctcc ccggtggaca
     3241 gatcaaatgg tacatgccaa tgatatttca ttttattatc gattgccggc tgataaccgc
     3301 gacctttttc cgtatggacg tgaagtacaa tcggatgatc aatatctttt acttcttcaa
     3361 ataaacggat caccgtttcc agatcgtttc cattatccac gtaacgataa tcaaatccca
     3421 ttgctttaaa taggttattg ctgcttttac cgttggtttg acgaagctca gctaagtttt
     3481 tatataaact gccatgattc tcatcaatag acatctcatt atcgtttact aatacgatta
     3541 gattggattg taaaacacca ccattgttca atccttcaaa agctaagcca ccacttaggg
     3601 agccgtcacc gataaatgct accacatttc ctgattcttt cttcagatct ctcgctttag
     3661 ctaaacctgt tgctaagctg atagatgtag atgtgtgccc cactgtaaaa tagtcatgtt
     3721 tactttcatg ttggttggaa tatccgctca cttcacggta atgtcctttt tcaaaaccat
     3781 tttttcgacc agttaaaagc ttatgaccat aagactgatg ggaaacatcc caaacgattt
     3841 tatcctttgg tgaatcaaat acataatgaa acgcaatcgt taattctact acacctagat
     3901 tcggaccaac gtgcccacct acatcgctgt ccttttgcaa gattttttgg cgcatctctt
     3961 ctgccaactg tttcatttcc tcaacagata attttttgac atctgcgggg ctattgattg
     4021 catccaatac atgtaatact gtactcataa tacattctct cctccgtact atcttctttt
     4081 aatgaaaata gatttgttac actttatcta ttctactact tagagttgac tctaagtcaa
     4141 tagcatttta aaaaaatttc agagaaagct tgttatataa gtagatcttc gcacgaatca
     4201 ggactcaaat aaaaaccttc tacagacaat taaaaagctt tgtagaaggt ctcctaatta
     4261 aggtctcatc aagaaatatg tttttttaat aggtgaagtt cctgagcaat atgatacaaa
     4321 tggatgtttg caccttttaa tgagattgga tagatctttg tcttttcagt ttctaatcca
     4381 ttttgatagc tagcagcatc gcacaaaacc actctttttt tcgcacatag acattcatca
     4441 ggcaattctt ctaatgaacg aaataaaata ggatttattt cagcgatgcc ttcttccgca
     4501 tacaaatgaa attgggttcg ataaaaaaat tgtttgactg aactttctga aacaacaaaa
     4561 atttctattt cctgtctccc cgcttgttca ctgttgatca aagaaagact tttgacgaat
     4621 tttcgaatga aattttcatt ccatcttagt tggatttgat ttttcttttt ccactcttcc
     4681 aatatttcta caatttttcc gtacagaagt tgttcttttt ctgatagaag atagatcctt
     4741 tccggtaaaa atacttgtcc atctgcccac aacgtacgga caaattgtaa aaaggattgc
     4801 ttgaaaataa aattttctgc atcgtcgatt tcagcgatac taataatctg taaaatcaat
     4861 tcattcagtg ataggtcatt gagaacaaaa tttttataaa caacttcgaa atcttttctt
     4921 aacagctcca tgttctcatt ggtataattt ctactgttga acaaagaaaa aatataatag
     4981 acatcttctt cttgtaagtc tagaattcct ttttgctctt ccaatccttg acagactaat
     5041 tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt ctttcagtcc aaggggaaat
     5101 gcctttcttc cgattgcgat ctctattcct cgtatgataa aggacttttc atcttccgaa
     5161 tatcttcttt taaaaaaatg agtctctaca taatcaatca actcgttaca agcttcttct
     5221 acatacggat ttttattttc atagccttcc caattgacat aacgtactaa agctagcaag
     5281 atgttccgcg agtcaaattc tgggataatg atttctttat ttttcaaata atcattttct
     5341 ttaaaaaaat ccaatacgat ttttttgatt gtatatactt tgctcaaaga aataaattca
     5401 agatcagcca gttctgtact cgtgaatata ccattaaaat aataacttaa aaactttaaa
     5461 aaataagatt gaccgtacag aaactgtgtg agctcagaaa gagcaatacc atgtttgaat
     5521 acgagtttat acttatgctt ctctcttact tgttctttta tacaatattc tagacactcg
     5581 gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca cccttagaga aatagctgtt
     5641 tgttcgatgt ctatttcttg gaattcaaat aattgttcac acagatacac ttttctaaat
     5701 atatttcgct cgatgtaatt ttccagcatt cctgatacac gcctccagtt tttcttatga
     5761 ttttatttaa tcatatctgt aaatcgtatt tttgtttcct ttttatttag aaaagccctt
     5821 tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa caagctcaca atttcagcct
     5881 cattgtaggt agctttgagc cgtttggact tttaccccag cctctttcaa gaatggttat
     5941 tcaaagatgt cagcgtctag ttctttgact acatctttca acaattggat accagctgcg
     6001 ctgttaccaa atggatctag tgccgggctg aagacaccta tgccgtaacg atttggcaca
     6061 gctgccatca acccgccgcc tacaccgctt tttgcaggta caccaacatg gacggaaaac
     6121 tctcctgatt catcatacaa accagcagtt gtcataattg atttgacgat tgtagcactt
     6181 tcttcagtaa tgatttgttt gccattccaa ggagcaattc ctttatttgc cagcacagca
     6241 gctaattttg ctatgcctgt agcggtgaca ttgagggagc attgtctgaa ataagcatcg
     6301 agtatctccg gtacgtctcc ttcgatcatt tgattacctt tcatatagta agcaagagag
     6361 cgattgatat cacctgttct tgattcagaa tgatagattt cttcattcaa tgtgatatta
     6421 ggatcatcac agatctcttt cataaatgac aagattcgct caaatttttc atctgcatct
     6481 ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat taacaaaagg attcattgga
     6541 tgtttgcgat gattgatttc catattcatg atagaattga aggcaaaacc ggttggttcc
     6601 gtgttgattg tttgaaagac tttttctatt ccgttgtctt tgattgctaa taacaatacg
     6661 ggaacttttg acatgctttc gatggcaaag cgcacatcgg cgtctcctcc ctcaatcagt
     6721 tgttttgtat ccaaatcata aatcgccata cctaattgtt tgggatccac ttcagctaat
     6781 gctggaatgt aatccgccac ttgtcctgtt tgataatagt ggaaattttt tccaatcaat
     6841 tgttctaatt cttttacttc catttgaata accagctttc attttttatt tttctgcatg
     6901 ctggaattta tgacggcatg cgtagatcgt aaatggaata actaagacga taagataaga
     6961 aacaatcaga atcgtttcat aaatcccatg ctgtccagct gcgatactag caggtggaac
     7021 aaatgagata caaaaagcaa aaagtgaagt caaaagacct aaacaagcaa cgattacttt
     7081 tacaactttt ccgcctggaa cctgataggc tctttcaaga ctgtcatctt taaaaaccag
     7141 cttcaaatag cccgcgaaaa acataaagta caacatcagg tagattacaa cagtcaatga
     7201 catcgctgca agaaatgaaa gattattccc cccgccgctt aatgtaaaga cggctgccca
     7261 gattgttgca acgatcccat ttgcgatcat caaatgagca ggaacaccgt gtgtgttttc
     7321 ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct acgtgaagtg cttttgaagg
     7381 agaggttacc caagaagcaa tttctccgat gacacctaaa caaacaagaa tcgcaagaat
     7441 tttcgcaatc catgcaagat ttggatcaat gtgtcttaat aggaattcta acgattgaat
     7501 cacccctgta ttaagagaca aaccatgttg aggtactgta gtagcgacgg tcaatccgcc
     7561 aaaagtatct aaaatgattg ccagaataac caatacaaac attgctaatg gataatcctt
     7621 tttagggttt tccatttcat ttgcatgact ggcagatgtt tctacgccca tatatgctaa
     7681 aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa aaatcaggaa tcaatgtttt
     7741 catagacagt atcgtttgta gtggagcgcc tgaagcgaca taatggatcc ctaagccaaa
     7801 caagactaat gctggcaaca agataccagc tacaaaaccg atccgagcaa tcaatgaagt
     7861 gtatttggtt ccttttaact gtgagagtaa aatagcccag aaaataatca ataggattgc
     7921 caatttcaga tatttattgt tttgtatagc accaataccc gttgcatagg ataatgcacc
     7981 agttaagaaa tacagcatgg tcaagtagcc aactgttatc tcaaaccatt ggaaaaagat
     8041 tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc caagtataga caccgccttt
     8101 ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat gccacaggga taaaccatag
     8161 caaaccgcct aataacaaga aaaatactaa gctgaagcct gaggtcgcga atgtgggata
     8221 ttcatataat gaaacgacca ttgagatggt catagccaaa aagccaaaga acgttaattt
     8281 ttttgcagga attttcgttt tgctttgttg catgaatctt cctcttttct atttgaattt
     8341 gtttcaattg agtttgttgc caaagaagaa aaagtccaat ccttcttctt gttcttcttt
     8401 tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa atcactgttg tcagcacata
     8461 ggcatacacg gcagtaatga tgacaccaat cacttgaatc cctaagaagt accagctgct
     8521 tggattcgtt agtaaatggc tgttagatgc aaagatccct atcagaatcg tacctgtgaa
     8581 tccgcccatg ccatgaacac cccacacatc aagtgcatca tcccattgtt tctttttacg
     8641 aaaatcaaca gcagcatgac agacgatacc cgcaatgatc ccaatcagca tggcactttt
     8701 tgcttctaca taaccggcac aaggcgtaat ggctgccaat ccagcaacgg atcctgttag
     8761 tacgtcgaca aaatcaaagc catttccttt tacttttgca atgatcagcc aagtaatcat
     8821 cgcaaatgca agagcgatga atgttgaacc aaatgcattg actgcttgtt catctgctcg
     8881 aagtgcacca ccggaattaa aaccgaacca gccaaaccaa agcagtcctg taccgatagc
     8941 agctgccata agactgctat gctgacttct ttctcctaaa ggaatctttc tttgaccaag
     9001 tgtgaggata catgctaaag agccgaatcc agctgtcgtg tggatgactg ctccaccagc
     9061 gaaatctcta aatcccatct gatccaaaaa tccgccaccc catatccagt ggcaaacagg
     9121 gaaataaatc agcagattcc aaaaaataac aagtataata tagcctttta aattcaatct
     9181 ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg ataaacatca gttggtaaag
     9241 aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg ttggggaagc ttgtaacatg
     9301 tctcatcaaa aagaaatctg ccgggtttcc aataacaccg ccgatatccc gtccaaaggc
     9361 caatccgaat ccaccaaata cccacataat cgttgttatc ccaacggaga gaaaagattg
     9421 agacatgatc gttagtatat tattcttccg tgctaatccg ccgtaaaaaa aagctagacc
     9481 aggagtcatc aggcaaacca ttgctgtaca taaaatcatg aatgctgtat ctcctgtatt
     9541 gataggtaat gtattcattt ttttcgcctc ctttctacga ttacgagtat aaaagaaggt
     9601 gtagttgttt ttttcttctg tttttttgaa ttaatacttg catttataaa agacagacaa
     9661 ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc acacaaataa agacaaaaaa
     9721 taaacggtct tctttttata gaaaaccgtt tattttcgtc tttttttatt tttaaagata
     9781 ttttttcata aggaataaag aggcggattt tataattaac ttagccattt ggaaataaaa
     9841 aaacgccacg tgaattccat gttatattga agttaccaca actctcaata gacaaggatg
     9901 aattcacatg acgcaaaacc atgataacac agtttcacgc aagggaaaac acctaaccta
     9961 ctcagaacgt tgccaaatcg ccatcctgaa aaaggaaaac tattccaacc gtcaggttgc
    10021 ggatgtcctg ggctgcgtgc cgcagaccat aaacaacgaa atcaaacgcg ggaccgttac
    10081 tcagcttaac cgccagaatc agaacgggaa ggtctacgac tattatcatg acgtctacga
    10141 cgccgatgcc gggcaggcgg cctacgagaa gaaccgcttg aactgcggca gacggccctt
    10201 atgggccgac acggacgcct tcattgaatg ggcggacgac atgatgctgt tggaaaaatg
    10261 gtccccggac gtggtggtgg gctttgccag aaaacacgaa ttgttcgacc cggccattat
    10321 ccctgggacc accaccctct atcagtggat cgaccgtggc atcatgaaaa cgaagaatat
    10381 ggacctgcta gaaaaactgt cccgcaaacc gaaatcgtcc cgtcatggca accgcccgaa
    10441 taaacgggtg ctagggaaat ccattgaaga gcgcccggag gcggttggta cccggaaaac
    10501 cttcggccat tgggaagtcg acacggtcgt aggaaacaag accaagtcgg atgccgtgct
    10561 gctgacgttg gtggaacggc agacacgctt cgaggtcatt ctgaagctgg ccgggaagga
    10621 tgccgaatcg gtcaaccaag cggtccgaca gctgagagaa cgggctggtg cgtacttcgg
    10681 ccagctgttc aagacggtta cctcggacaa cggctcggag ttcgcgggac tgcacgatgc
    10741 cttgcaggat gtggcaaaca tctacttcag ccacccctat gcttcctggg agcgcgggac
    10801 gagcgaaaac caacacaagc tgattcgccg tttcatcccc aagggcagcc caatcggcaa
    10861 cgtgagcgaa aaccaaatct tgcggattca gcgttggatg aacgattacc cgcgaaagat
    10921 tttgggctat gcaacgccgc atgacacgtt cgtccaggca tttaggcagg aacggttgac
    10981 ggcttgacac gtgggccctt gacaacgagc ctccctcggc atggcttgtg tctggaaagc
    11041 tggccggggc cagccagtcg ggagactacc atgcctcagc tcattgtcaa gggcccactc
    11101 gattagaaaa attagacggt tggtggtaac gagcaaccgg gtcaccatgg gtggctaact
    11161 taaacttgaa atttacgtaa ggaataaaga atacttcata taaaatatat aaaaaagaac
    11221 cgcagcgagt gtcgctacgg ttctcaaatt tgttcttcat ttcaacataa gtttttcttc
    11281 aatttgctta taatttacgt gcaaagaatg aaacgactaa tactaaaatg attgcaccga
    11341 tcaatgatgg aatgatagcc attcctgcaa ttgttggtcc ccaagttcct agtaaagctt
    11401 gtccaattgc tgaacctact aaaccagcaa tgatgtttgc gatgatcccc attgatgatc
    11461 ctttattcgt gattgctcct gcaattgctc caatgatacc gccgacgatt aatgcccaaa
    11521 taaatcccat tttaaattcc tcctaactga cttcttactt cttattgtac acgtggttcg
    11581 ttattttctt tgactttttg tgcacctttg cctacagcag attttgcttt atctgtttgg
    11641 cgtgaagcaa attgtcctgt ggattttgct gcttctgtta ctttgtcttg tactgtttct
    11701 tgatcttttt ggtattcttc ttgtgttttg atgtctgcaa cattcacatt cacttcgata
    11761 acatctaaat gtgtcatatt gtttacttca gtactgatca attctttgat ttggttgtaa
    11821 atatcttcga tgtctttacc gtattctacg atcacatcta gatccacagc tacttgtttc
    11881 ttacctactt ctgtattgat acctgctgtt acgttatctg tgttgactaa tttttcagcc
    11941 atatttgaga aaaatccgcc atctaatgtc aatagaccat ctacatcttt caatgcataa
    12001 ccgatgattt tttggatgac tttgtcttca taagtcaatt caccattcat catgcggttt
    12061 tctccttttt catttgccaa aactgatcca tttgttgtat tcatgttttt gttttccatc
    12121 tttgtttcct cctatgattt aaaaaattga tcaactattc ctgactgttt caaataaaaa
    12181 ccgattcctg cgccaattgc tgtcatgatt atgatcaaca atgtcttgaa aaatccaatc
    12241 gttaaccata aaacagctag cagtagtcca agacccccaa agataatagg gagcttgtat
    12301 gctgaaaata gttctttcat tatttggccc tcctattcca ctcgagagtg cttgtagtct
    12361 cttgtatctt cttgttcttc ataaccgctg tatgtgactg ccactttgac cgtttctttt
    12421 gttccgagta ctttagttac ttcttcttcg atatcttgca tcaatacacc tgttttccct
    12481 actaatgaag aagttcgttt caagtctccg ttgacatgga tatggattcg atttttcgtt
    12541 gaacgtatcc gtactttagg tgaatgaatg aactcttctt cgtgtaaatg ggaacgtacc
    12601 aacccttcga tcgctttttt gtcgattgtt agtttgccat cttcccgttt cataacgaaa
    12661 gttcctcttg actttggata gaaaagaacg actagcaaga tgataatagc aagaacggca
    12721 aatactacag ctgcccagaa aatatattgt tgcatgtagt aattcgttaa agtaaagaag
    12781 cgaaatcgtt ccaattgttc aggcataata tacggcgcat tgatcaacgc aatcgctaga
    12841 aatactgaaa gtagcagtaa actcatgata acaccgatta tttttaaccc cttattcatt
    12901 ttccacctcc tataaactta tttttaccct gtaaataact ctaccgacaa tcacagtata
    12961 gatagcgaac agttagcata gcatcagtaa ataaagcgct accaaacatt gctggatcat
    13021 aacggcgttt tattgccagt ctgatactga aataacaatt gcttacttat tctctattta
    13081 cttttgattg ctggctatac ttaaaggata ttattcaaga ttaaaaattg caaagattat
    13141 gctcagttat tattacaaga ttctatttat t
//
LOCUS       DAYYBH010000070        13096 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000070 DAYYBH010000000
VERSION     DAYYBH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.073"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACHAUG_002224"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACHAUG_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACHAUG_002225"
     CDS             706..1734
                     /locus_tag="ACHAUG_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8481305.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACHAUG_002226"
     CDS             1731..2489
                     /locus_tag="ACHAUG_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8481306.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACHAUG_002227"
     CDS             complement(2575..3315)
                     /locus_tag="ACHAUG_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8481307.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACHAUG_002228"
     CDS             3376..4629
                     /locus_tag="ACHAUG_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8481308.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACHAUG_002229"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACHAUG_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACHAUG_002230"
     CDS             5300..5626
                     /locus_tag="ACHAUG_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8481309.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACHAUG_002231"
     CDS             5694..6407
                     /locus_tag="ACHAUG_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8481310.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACHAUG_002232"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACHAUG_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACHAUG_002233"
     CDS             7690..9555
                     /locus_tag="ACHAUG_002233"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8481311.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACHAUG_002234"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACHAUG_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACHAUG_002235"
     CDS             11120..11821
                     /locus_tag="ACHAUG_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8481312.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACHAUG_002236"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACHAUG_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACHAUG_002237"
     CDS             complement(12857..13066)
                     /locus_tag="ACHAUG_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8481313.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         4396 a         2198 c         2765 g         3737 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagta
//
LOCUS       DAYYBH010000071        13018 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000071 DAYYBH010000000
VERSION     DAYYBH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.074"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..491
                     /locus_tag="ACHAUG_002238"
     CDS             18..491
                     /locus_tag="ACHAUG_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8481314.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            500..727
                     /locus_tag="ACHAUG_002239"
     CDS             500..727
                     /locus_tag="ACHAUG_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481315.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1363..1734
                     /locus_tag="ACHAUG_002240"
     CDS             1363..1734
                     /locus_tag="ACHAUG_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481316.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1990..2232)
                     /locus_tag="ACHAUG_002241"
     CDS             complement(1990..2232)
                     /locus_tag="ACHAUG_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8481317.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2264..3166)
                     /locus_tag="ACHAUG_002242"
     CDS             complement(2264..3166)
                     /locus_tag="ACHAUG_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8481318.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3179..3367)
                     /locus_tag="ACHAUG_002243"
     CDS             complement(3179..3367)
                     /locus_tag="ACHAUG_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8481319.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3381..3944)
                     /gene="amaP"
                     /locus_tag="ACHAUG_002244"
     CDS             complement(3381..3944)
                     /gene="amaP"
                     /locus_tag="ACHAUG_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8481320.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3982..4875)
                     /locus_tag="ACHAUG_002245"
     CDS             complement(3982..4875)
                     /locus_tag="ACHAUG_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8481321.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4953..5876)
                     /locus_tag="ACHAUG_002246"
     CDS             complement(4953..5876)
                     /locus_tag="ACHAUG_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8481322.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5925..6275)
                     /locus_tag="ACHAUG_002247"
     CDS             complement(5925..6275)
                     /locus_tag="ACHAUG_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8481323.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6308..7210)
                     /locus_tag="ACHAUG_002248"
     CDS             complement(6308..7210)
                     /locus_tag="ACHAUG_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8481324.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7203..8060)
                     /locus_tag="ACHAUG_002249"
     CDS             complement(7203..8060)
                     /locus_tag="ACHAUG_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8481325.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8394..9203
                     /locus_tag="ACHAUG_002250"
     CDS             8394..9203
                     /locus_tag="ACHAUG_002250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8481326.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9243..9740
                     /locus_tag="ACHAUG_002251"
     CDS             9243..9740
                     /locus_tag="ACHAUG_002251"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8481327.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10125..10198)
                     /locus_tag="ACHAUG_002252"
     tRNA            complement(10125..10198)
                     /locus_tag="ACHAUG_002252"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10162..10164),aa:Ile,seq:cat)
     gene            10385..10708
                     /locus_tag="ACHAUG_002253"
     CDS             10385..10708
                     /locus_tag="ACHAUG_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481328.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10872..11126
                     /locus_tag="ACHAUG_002254"
     CDS             10872..11126
                     /locus_tag="ACHAUG_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481329.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11196..11441
                     /locus_tag="ACHAUG_002255"
     CDS             11196..11441
                     /locus_tag="ACHAUG_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8481330.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11517..11882
                     /locus_tag="ACHAUG_002256"
     CDS             11517..11882
                     /locus_tag="ACHAUG_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8481331.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11943..12506)
                     /locus_tag="ACHAUG_002257"
     CDS             complement(11943..12506)
                     /locus_tag="ACHAUG_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481332.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         3778 a         2428 c         2192 g         4620 t
ORIGIN      
        1 ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta
       61 taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa
      121 aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc
      181 tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc
      241 aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta
      301 ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg
      361 ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat
      421 taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt
      481 tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa
      541 aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat
      601 tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt
      661 tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga
      721 agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga
      781 gacttttaga agttatttac gtctattatt cagatttttt atttcgattg aataccgttt
      841 tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact
      901 atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat
      961 aatactttat tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt
     1021 attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg
     1081 tgttaactct tactccattc ttttttagta agctcccata tatactgtaa agccttctat
     1141 atattatgtc atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata
     1201 acagtgatgg acaattcttt tttattttta acattatttt gataataatg attatttatt
     1261 taaaaataca ttagataaca tttgttacga cacgaaaaag cttcacaaac aaatatcgtt
     1321 attttaaaat gagggcgaac tcacacaatt aggaggaata gtatgccatt tattgtttac
     1381 ttttttcttt cattactcgt gatctatatt ccattaccaa catttttcct gctcgtcaat
     1441 cgtctagcag agcaacatga tacgattgct gctcttacga aactttgttt acttctattt
     1501 gtttgtctag attactttac gagctattat tggctaaaac agacaagagt aaaaaagaga
     1561 tattatttct tccttgtctt tttcaatctc tttgagtttc tgatccattg ctttttactt
     1621 catcagtatc atacgatcca tttgtggatc atctgggttt tcaaagtggt cgtattattt
     1681 tgtatgttca cctttcctat ctcaagaaaa tttagggagg cattgtttta ttaaagaagc
     1741 ttctctgtat caataaagac acatttaagg aaaaactttt aaaaatctaa ttgggaattt
     1801 gcccgcataa ataagaggct gtgacaaaag tcttgtcaca gcctcttatt ccgtataaac
     1861 agtgttcaaa agcttgcatc ttcgataata tgcccaacca aatgaaaaat atgagaagct
     1921 atttttgttt ggctgttctt attacttgat gaagaacagc tttttcacga cctcttttta
     1981 aaagtctttt tacagttttc gagcaaagaa tgatacgatt agtactaaaa tgatggcacc
     2041 aatcaatgat ggcacgattg ccattcctgc aatacttggt ccccaagaac ctaataatgc
     2101 ttgaccaagt gctgatccaa ctaaacctgc tacgacattt gcaataatcc ccatcgaaga
     2161 acctttattc gtgattgctc ctgcaattgc tccgataatt cctccgacga taagtgacca
     2221 aataaatccc atgtttcgtt cctcctaatg ttttacttcc ttattactga acccgtggtt
     2281 ctgtattttc ttttactttt tgtgcgccgc ttccagctgc tgattttact ttatctgttt
     2341 gattcgaagc aaattcaccg gtttttttcg ctgcgtcagt tactttgtct tgcaccgttt
     2401 cttgatcttt ttcaaattct tcttttgttt tgatgtccac tacgttagcg ttgacttcga
     2461 tcacttctaa atgtgtcata ttttttactt ctttgccgat tacttcttgc atttcttcaa
     2521 agattttttc gatatctttg ccatattcca caatgacatc catatcaact gctacttgtt
     2581 ttttaccgac ttctgtttcg atcccagccg ttacattatc tgtattcaca agttttcctg
     2641 ccacattaga gaagaaacca ccgtttgcag ataataaacc gtctacttgt tcaattgcga
     2701 ttccgatgat tttttgcaca actttatctt cataattcag ctcaccgcgc attgaatctt
     2761 cctgttcaga atcatgcttt ccttggttat ttgtatgatc agaaccaagt tttccttgtc
     2821 cgtttgtgtg gtcagaacta agtttttctt gtgctttcgc atggtcagaa ggatgttttt
     2881 gctcttccac aggtttagaa ggatcgattg catcatgttg ttctggaact ttagattctg
     2941 gagttcctgg atgatgaggt gcatgctttt ctgcatcatg tttcttctct tctactcctt
     3001 gttgaacgtt agggactact ggatctggat gtgcagatac tggtgctgtt ttgttctttt
     3061 catcgttcac accatttgat tggtttggtg tcgttgggtc tggatgtgca gacactggtt
     3121 tttggttgtt cattttattg ttattatttt tgttattgtt attcatggat gtttcctcct
     3181 aagatttaaa atagtgatct aagattttcg tttgtttcag ataaaaaccg atagcgacgc
     3241 cgatcgctgc catgatcaag atcaataagg tcttgaaaaa gcccacggtg atccataaaa
     3301 cggctaatat caatccaagt accccaaaga tgatagggag cttgtatgtc gaaaatagtt
     3361 ctttcatttt tggtcccccc ctattctact cgtttgcggt cattgcttcg tttagctggt
     3421 tcttcaaagc ctgtgtattt caccgatact ttcactggct cttttgttcc caatatgtgt
     3481 tgtacttcat tttcgatttc tttcatcaat gttcctgttt taccaatgag tgctgaagtt
     3541 cgtttcaact ccccttttac attgacggcg atcttgtttt tcgttgcttt gacatttact
     3601 tttggtgaat ggatgaattc atcttcatgc aagtgagaac gaaccattcc ttcaattgct
     3661 tttttatcca acgtcaactc cccatcacca tgtttcagta gaaagtttcc gattgttttt
     3721 ggataaaaca ggaaaacaag taacgtgata attgtcaaaa tagcaaatgc aacggcaagc
     3781 caaaatacat attgttgcac gtatgaatta gtgatggaaa agaatcgaaa cttctctagc
     3841 tgaaaaggca atggataggt tgcacttaat agtatcgcaa acaaaagcac agatagcaaa
     3901 agtaatgttc cgatcactcc gatcgttttt acacctttat tcattttcgc acctcctcat
     3961 aggggtgaaa cgatcaactt cttataagtt gatcgcttct ccaccagtta ctccgtaaat
     4021 ttgggctgta acataacttg ctttattcga tgctaagaaa acataaactg gtgctaattc
     4081 tcctggctga cctgcacgtt ctagtaaact ttgctgacca aattcaggga tcgaatcaga
     4141 aggttgtcct tcgtctagtt gtaatggtgt ccagattgga ccaggtgcta caccatttac
     4201 acggattcct ttaggtgcaa gctgttttgc taaaccgatc gtaaagttag cgattgaagc
     4261 ttttgttgct gcgtaatcta ataaatgttc gcttggattg aatgcttgaa cagaagttgt
     4321 tgttacgatt gctcctcctg ctgggatatg cggaacagct tctttcacta aagcaaacat
     4381 agcgatgatg tttatcataa atgtgtcttt gacttgctca agtggtaact cagcaataga
     4441 ctgacaagaa atttgctgtc ctgcgtttag taccatcgta tctagcccgc caagttcact
     4501 cacggcttta tctatgattt ctttaggtgc ttgttcgtca cgaagatcaa aaggtagtaa
     4561 gacaatctta cgtcctgcat ctttcacgta ttttgctact tcctctgcat ctttttcttc
     4621 tcctgggaag aattgaagag cgatatctgc cccttctcta gcaaatgcaa tcgctgctgc
     4681 acgaccgatc cctgaatctc caccagtgat tagtacacgg cggttttcta gttgattatt
     4741 gccttcatag gattcttctc cacaatcagg aacaggaatc atattctgct gtaaagctgg
     4801 tgtatcctga tcttgtttag gaaatttctc cgtatggtac aagtttcgcg ggtcgattaa
     4861 ttttggttct gtcattgctg tattcctcct agtttttaaa ttgtattgcc aaaacaaatt
     4921 tctttgtttg atacctgttt taaatagatt ctttaatttg tataatcttt ccggtataaa
     4981 tggtaaccta aaactactgc tgctatcaaa gagccaaaga ctacgagtat aaagcctaga
     5041 gcagaacttt tacccggtaa tcctcctgta ttcattcccc aaatccctgc gaacaacgcc
     5101 ggaacggaaa tgattaatgc agctgaatct agatatttca tgatatgatt tagattattg
     5161 tccatcatcc cgttgaatag atcaccaata ctctctaata aatctcgata gatctcgatc
     5221 attttttctg tttgtcgctg tctcaaatgg acatcataga gtagctgttc atcgtctaaa
     5281 cgtttgatga aagtttcaga attccataat tgatccattg tttcccgttg atctcttaag
     5341 gtatgatcga tatatacgat gtctctttca agatctgcct gtttatacag ttcttcattt
     5401 tccgttgttt tacgagctgc tttatccaaa gcgtcaattc gctttttcat ctctcttagt
     5461 tctttcacgt aatgggtgta aatatctaat aatgtatacg tgatgatctt ttcaaacgag
     5521 tttaaatgat ttccatgctt tgtcagcagc cgatccacaa attcggtttt tctatcgata
     5581 caagtaatca agagattatc ggaaagtaca aaagatattg gcgttaattt tttttcgata
     5641 cgattatctg actgatctag attaatcact accaaagaaa atggatgctc tagtttcgtc
     5701 tcataaaggc gctccatccg agaaacttct tccggatatt cggttcctac gaaaatatca
     5761 tcaggaagct cgaattcttc gattgcttgc tcaatctctt cgggctttgc actatcaagt
     5821 acgagccaat tatatttttt ttcatctgtt tctattacat tcttttcttc atccacaaag
     5881 tagtatctca atgtatacct cctttttgga ctttctcgaa actattagct gaagaagcag
     5941 ttctttaatt tgatattata atcgagtact gcgatttcat tttctaatga aaagctgtca
     6001 agctgtttgt tgaagtccat gttcaatcgt ttaccgaaat aattttgctc taatttagat
     6061 gtatagatgt ccaataatgg atgctcgatt ttagtcgcat attctccaaa ttccgaaaca
     6121 aaaggaatga tttgaaaaaa gctgcctttt gcaaaagcac ctttgtccct tagaaatgga
     6181 tcggaatcat tgataagcgc gagtgcgatt tttgtatttt ccccaacctt ttcaattaac
     6241 gtctctactg catttttaga cattgtaatt tccattccta ctccccctct tcagttgttg
     6301 tcttctatta tttaacaact acatctgttt ttggttgaac ttttgcatat tttggatatt
     6361 tagggaattt agctacacaa cggaaagccg tcatccctgc atatttttcg taatatttct
     6421 tcgttggccc ttcaaaatcc ttgctccacg gtttcacatg tcctgaaaaa tggataatgt
     6481 aaggattgtt gcgagtttct tcgtattcac gttcacctgc tgctgttgga tgtttttttg
     6541 cttttgccat gatatagcct tgtgcattcc aacgcggatg aagtggtaac caacggtcat
     6601 gtaagatagc atttaatgca tcttggtcat ggaatcgtaa tttttctgga ttatgttcga
     6661 tgaaatctaa tactttttgc gtaatatttt catctaacca ttttttcaca ttgatcagca
     6721 tcaaacctga gttgaaataa cgcattgatt tagctgaaat tttcattttt tccagtcgtt
     6781 gatggaaccc agcgtcttca acagctgcta cgattgaatc accgaagtca agacgccata
     6841 atttagagat atcttgtaaa gcgatcatgt cgcaatccat atataagata cgttcaactt
     6901 ctgtgccgcg gaacaattcc ggaatcgcaa tacgataata agctgtttct ggaatacgat
     6961 cgctgattac cacatttgta aagaattctt tattgatttt caagaattcc acatctgagt
     7021 tcattcgtgc gtttttcact gagaagcgta aaagctgttt actttcaaaa tcgatatcat
     7081 cgtcaatcac atagaaccga acaaatttag acgggttgca attatctaat actgagacaa
     7141 ataatgcagc taaatgtggt acgaattttg tgttacaact tgcgactact gctatttctt
     7201 ttttattcat tgatctcgcc tactttcgtt agagtactgt gtgctaattt ttttaagtat
     7261 aaattggtat acggatgatc tttcaacgta ttccaaggtt tatcatgtcc tgtaaagtga
     7321 acgatcgaag gtttttcatt tccttcttta tactgacgtt catatttttc atttggtgct
     7381 ggatgacgtt caaaaatcag cgaagtctgc atgttccatt ttggatgaag ttgttcccaa
     7441 tcttcataaa gtacagcatt caaagcatct tggtcgtgat agatgattcg atcgccattt
     7501 tcactgaggt aatggatagt tttttctgta atttcttttt tattccattg atcaatgtca
     7561 atcaccatca ctcctgaatt gaagtacaag tcatcggaat cgattcctaa tctttccaaa
     7621 gctttcactt ggcctggatc gataatagcg ccgattgttt ttccatttaa cggctcatca
     7681 tatagtttta cgatatcgtc tagtactaac acatctgaat ctaagtaaag gactttctta
     7741 taatcctctt tagctaataa gtttggtaaa gaaattctta agtaagcggt agttgtgata
     7801 tggtcactga ctaggaagtc ttcgtagata tctttttcca ctgtcaggaa ttggatactt
     7861 gcattacttg aatatttatt taccgtttct tccaatcctt ctttactata ttctgataga
     7921 ccatcgtcaa tcacataaaa ttttatgcgt cttgttttat tgcaattttc taatgcagtt
     7981 gcgatcatca cgctcaagta aggagcataa ttttcgtctg aagcagttac cacaggaact
     8041 acgccgtatc tcttctccat caaaatcact cctttttcta atctttttct aacctgttct
     8101 acatctagaa tattcctcat ttggttttaa agcaaaatat atgctttgtt ttcattgaat
     8161 attttaaaat ttttttatca caaaacgcat cggtaagcgc taactattcg tttttcatgg
     8221 ctttttattc aaaggtattg accaaaaact actatatata gccacctttt tcttaagaat
     8281 gttttttgaa aaaattcata atgacaaagc aagttagttt cattttttta aattctcatt
     8341 taatcttaag gtaacaggaa aatagatatg taaaaagaaa gtaggaagat aaaatgaaac
     8401 tagcagcgat tgatttagac ggtaccttgc ttgatcgaaa tggaaagata ccagaagaaa
     8461 atatccaagc acttcggaaa tttgatgaaa atggcggaat cgtaaccgtc gctaccggaa
     8521 ggaacagcat ttctgctaaa gatatttttt ccgaattagg aatcggtggc tatttgattt
     8581 cttccaatgg ttcactgatt gctgaaatga aggatggaaa aatggcgaaa gttctcagac
     8641 gttcaaaaat agatagcgat acactgagaa aagccttctt ctatgctcaa gaagcgaaaa
     8701 tatccatcat tgctagccga gaaacccaag atgatcagct gacattcgat gaatcaaatc
     8761 tagtcaaaga tgacccttat tatgaacatt tcaatttaca gaaccattct tttgatcaaa
     8821 ttttaaatat gttggaagat ccttctctta gctatcttaa attagcatta acagataaga
     8881 gtgaagaaaa actacagaag ctacaaagag atctgcaagc agatggtatc caatcagtat
     8941 tctctgatcc gcatttctta gaaatcacgc ctacaggtat cactaaagct cattcacttg
     9001 tttttctggc ggattacctt ggaatcgaat cgagtgacat catggctttt ggcgatcaag
     9061 aaaacgacat tgctatgttg aattacagcg ggttgagtat tgcgatgggg aatgcacagc
     9121 gacaagtaaa agaaattgca aaccaagtaa cactgacgaa tgaagaagct ggtgttgctt
     9181 atattttaaa aaacttctat taaaaagaat aaaatgacga tatgaatgta ggaggaaaga
     9241 aaatgttagt tattattgat atgcaaaatc atattcttga tccaaacagc gaattttata
     9301 ttccagagtc agatcaattg gcagaacgta ttgctcaaag attagctaaa gcacgccaaa
     9361 ataatgaata tattctcttc acacgggata taccagttga actgaaggac aaagaagaag
     9421 aacgagaaga tttgcaattg atccctcttc tttctccaca agaaaatgaa cgagtgatta
     9481 aaaagtatta ctttacgatt ccacctgaaa ctttagctga aatccaggaa actttatttg
     9541 aaagcaaaga agaacaaaaa gagatcgaag tagtcgggat tgaaacaaac ttgtgtgtac
     9601 tctctaatct gataggcctg cagagtgctt ttcctgaagc agactttttt gttgatccaa
     9661 tgttagtctc cagtagaaaa cacggagaat ccgctttaga gctgttgaaa gatttcaacg
     9721 tctctatcgt cgaatcataa aaatatctat cttcatttac gatatttaac acacaataaa
     9781 aaaagcacgc agaactgttt tagtcctacg tgctttttct atcaaagtag attggctttt
     9841 ttatgacaca gagtgaaacg gcaggctcac gacctctttc ttaaatggtg ttcaatcagc
     9901 tgaccctcgg tattagctta acaaacggca aaatatgagg aactattttt ccgtttgtta
     9961 agctaatact caggtcaaaa cgctgatttc acaacctctt ccttaaacgg tattcaaaag
    10021 cttgcatctg cagtaataag aacagccaag taaaaacata agaagctgtt tttacttggc
    10081 tgttcttatt acttgatgca gggcagcttt ttcacaacct cttatgtacc ttgtaggact
    10141 cgaacctacg accggacggt tatgagccgt ctgctctaac caactgagct aaaggtacat
    10201 cataaataaa atttattttt gttacttttc aactatagca cgaatgacaa actctcacaa
    10261 gtttcaaaat ataagtaaaa taatattgtt attcttattt cttttacttg tgtcataact
    10321 attttgttac tttaattttt atttgcttat tttagaaaaa atgttattct tttatttatt
    10381 ctcaatgtta ggagtgatta ttttgccatt tattgcgatt ttattcgatt tagttgcggc
    10441 agcagcttat tttctccaat taaattatca aacctcagga attctgttta ttggaatgat
    10501 ttttcaaggt gtgattaccc ttttgcttct tgtcatgatg ctcagctata aaggaaaaag
    10561 atatgcccgt attcaaacaa aagtatttgt taaatatgtt tccattcgct atgggatcat
    10621 cattctttcc tttttagtga atgcgatcgt cctcttttta tatgtattga attacttaga
    10681 tatcaatcca ttgatttttt ctcgatagat aagacacatt cagcggaaca gttcctgctt
    10741 atcagcagaa aactgttctg ctttttcatt tttcattgat ctattcttct tttattttcc
    10801 tagcatttca tttgtgatgt atgcaataac atgttatctt ttattatatg aatcgttgag
    10861 gagtccgtta catgattacg ttggtgagag ttttgttttg ggtgcctgcg gtcgcactgg
    10921 tcgcaagtat tgtttatctt atgaattgga acaaagaacg gttttattta gctattttga
    10981 ctcttccggc tatctacttt atgtggaaag tttttaatta caactacttt gaacccgata
    11041 gtgtttttat agaagaatta tcaggactgg ttttgtcttt attgatcgtc attctttatt
    11101 tgatacgttt aaataaaaaa cattgagtac tattgattgc atgtgtagct ttctatagtc
    11161 attataacta ttctactata atgaaaggtg attaaatgag tatcgaagaa gctattttac
    11221 tggatacttt acgttgtcgt ctgaatttct taagaactgg ccgatcttta tctcaagtag
    11281 ctgtttctag cgcgttaagt attcctttat tcaaatatcg taagtacgaa gaaggagaag
    11341 aaattccaag catcattgaa ttaatctata tcgctgactt ttacgatatt tccgttgatt
    11401 acctaattgg tcgctcagaa acagcaagca aactgaagtg attttttcca gccttggtgc
    11461 tggttttttc atttaacgtc taaggttaac aatctatata taaaggagac atttctatgc
    11521 gattatggca tgaaaaattg atttcaaagc ttcctcgtca acagctttta ggacaacatc
    11581 gggaatgctg tgcattaaga ggaaatggct ggggaaagaa acatgctact gtggattatg
    11641 tttttcacta ttctccttat agattattcc aatatcatca gcttgtcatg gatgaaatgg
    11701 aaaaacgtgg ttacagccca gcaccagagt ggaaaaatcc tatgtatcgt ggcaaatcat
    11761 gcgaagcatt ttttgattta cctactgtag agcgcaacgt gccgatttat ccggaacacg
    11821 atgaagttta tctagcagaa tgtttagcaa atttggagca aaaaggaatt tatcttacct
    11881 aatataacta gaaaagcctt ctatctcctc ttacttcatt aaaaagcttt aaccatctta
    11941 gatcaagcta aaatgtcgta agttttaggg ttatcttcat cctcacgtgc aaaactaaaa
    12001 gcgtactcta gttttggttc atcgccaact tccctagaca taaatgaaca tctcctgacg
    12061 tggtttaaaa gtaccgcatc ttcgacgtct gtatacgatg cacagcacag attgcttctt
    12121 ttgatctttc ttttgatttt tctgttgctt cttctacacc ttcttcgatt actacatcga
    12181 tgcttacaat tgcttctgcc taatattccc atgcatcgtc gatttaagcc tctgtagtca
    12241 ctgtgatcgt ttccacattt taactcctct gtcgtggctt cagttgattt tgtgatcagt
    12301 tcagttgcta agccagagtc agaacaacct gccattgcta gcgttaatat tgatagtaaa
    12361 aatagttttt ttatcataag gttccccccc tgaaaataga ctaaaataat aacgctttct
    12421 ttttttgttt tcagacgaaa attaataatt tctaaatatt ctttaaaaaa gcttataact
    12481 attatgcctt ttttaatata taacacagtt atttttaaaa ataagaatgc taaatcactc
    12541 tacaataacg gcgaaagcac aaaaaataaa aaaattatta cgttattaaa aaaacagatg
    12601 aaagatcagg ttttattaga tctcattctt tcatcacttg aagggattta atgaactact
    12661 ttttcctctt tgtcgttttt atatattcgt attccttcaa acagtaggat agcctctttc
    12721 aatgcttgat atttgaactg tccgttactt aaaaatttag atgtgccgcc ctcttcctag
    12781 aacatattct tttttacttt ttcattaatt gacagaccaa tcgaacgaaa ctgtaaattt
    12841 cgataggata atgattggtc aaactcattc attttcaaga catctcgatt catattcaag
    12901 ataggaatct ttttatcaac atcactttat tcgtattttc ctctaccata aaagcttcac
    12961 gctccacaaa attgttttat acactactat tcagcccatt caacgaccaa tgaatttt
//
LOCUS       DAYYBH010000072        12875 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000072 DAYYBH010000000
VERSION     DAYYBH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.075"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      5..171
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            319..876
                     /locus_tag="ACHAUG_002258"
                     /pseudo
     CDS             319..876
                     /locus_tag="ACHAUG_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            934..2976
                     /locus_tag="ACHAUG_002259"
     CDS             934..2976
                     /locus_tag="ACHAUG_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8481333.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADDKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAE
                     IKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3085..4236)
                     /locus_tag="ACHAUG_002260"
     CDS             complement(3085..4236)
                     /locus_tag="ACHAUG_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8481334.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4390..4920)
                     /locus_tag="ACHAUG_002261"
     CDS             complement(4390..4920)
                     /locus_tag="ACHAUG_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8481335.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5019..5369)
                     /locus_tag="ACHAUG_002262"
     CDS             complement(5019..5369)
                     /locus_tag="ACHAUG_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8481336.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5526..7055)
                     /gene="ahpF"
                     /locus_tag="ACHAUG_002263"
     CDS             complement(5526..7055)
                     /gene="ahpF"
                     /locus_tag="ACHAUG_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8481337.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7065..7628)
                     /gene="ahpC"
                     /locus_tag="ACHAUG_002264"
     CDS             complement(7065..7628)
                     /gene="ahpC"
                     /locus_tag="ACHAUG_002264"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8481338.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7772..8434)
                     /locus_tag="ACHAUG_002265"
     CDS             complement(7772..8434)
                     /locus_tag="ACHAUG_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8481339.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8618..10264
                     /locus_tag="ACHAUG_002266"
     CDS             8618..10264
                     /locus_tag="ACHAUG_002266"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8481340.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10255..11337
                     /gene="uxuA"
                     /locus_tag="ACHAUG_002267"
     CDS             10255..11337
                     /gene="uxuA"
                     /locus_tag="ACHAUG_002267"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8481341.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11445..12875)
                     /locus_tag="ACHAUG_002268"
                     /pseudo
     CDS             complement(11445..12875)
                     /locus_tag="ACHAUG_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         4299 a         2367 c         2404 g         3805 t
ORIGIN      
        1 atagtaataa ccttgttagg tgaggctcct atacaaacat aggctgttgc ccagaaacgt
       61 cgaaagtcgc caatgggtaa aacagaaact gttggattaa ggctttttcc aatagctaag
      121 aatgttttct ttacgttgta tagtgctaaa actcggacga tgagatgatg ctaggtcaga
      181 attcttgttc gaaaaggttt ctgacctttt ctttatattt ttgtgactga cttatttgat
      241 gagacaaact cgctcctcac ctgacaagtg gattgctgtg tgaggaggag atgcggtgaa
      301 aaaagatcga ttggtggagg tcgctcggat tcacttctgg ttgcacaata caaccagaaa
      361 acacaacaag caaaactgat ctctatcatg agagattcat atgtcgatat tccaggttac
      421 ggaatgaata agataaatgc agcttactct tatgggggag ttgatttatt aaatcaaaca
      481 ttaaaggaaa atttcaaatt tgaagctccc tattatgcaa gtatcacatt tcaagatttt
      541 atcgattgcg ttaatgaact gtttcctggt ggagtcaaga tcgatgcaga aaaatctcta
      601 gatttagatg gcgtatatat aaacaaagga gagcaagtga tggatggtaa tactttactc
      661 cagtatgctc gatttcggga agacaaagaa ggagacttcg ggaggatcag aaggcagcag
      721 caagtgatca aagctatctc tcagcagctg aaagacgtaa cttcaatatt caagctaccc
      781 aaagcggttg ggaaattact tggtagtatc caaacgaacc ttccagaaag tgtgcttctg
      841 gattgtggta tggatttcct caaagacata tgttagaata aacaggtata aatagtgaaa
      901 taaaggaatg acaagcaaga gaaggaggaa aaaatgaaaa gaagtgacaa gcacggcaaa
      961 aatcgaacag gcgcttatat tgccggcgca gtgatcttaa tagcagctgc gggtggcggt
     1021 tatttttact accagcacta tcaagaaacc caagcagtag aagctggaga aaagacagtt
     1081 gagcaatttg tccaagcttt gaacaaggga gattataaca aagctgcaga aatgacatca
     1141 aaaaaggcgg caaataaaag tgcattatct gaaaaagaga tcttagataa ataccagaat
     1201 atatacggtg ctgctgatgt caaaggactt cagatatcga atctaaaagt agataaaaaa
     1261 gacgattcta cttacagttt ttcatataaa gcaaagatga atacctcatt aggtgaattg
     1321 aaagatcttt cttataaagg aacattagac agaaatgatg gtcaaaccac gatcaactgg
     1381 caacccaact tggtttttcc agaaatggaa ggaaatgaca aagtaagcct gaccacacaa
     1441 gaagcagcaa gagggaatat tatagatcga aatggtgaac cattagcaac aacaggcaaa
     1501 ctaaaacaat taggagtcgt tccaagtaaa cttggagatg gaggcgaaaa aacagccaat
     1561 atcaaagcga ttgcttcctc cttcgactta acagaagatg ctatcaatca ggcgatttca
     1621 caaagctggg tacaacccga ttactttgtc ccattgaaaa tcattgatgg agcaacgcca
     1681 gaacttccag ctggagctac cattcaagaa gtagacggca gatattatcc tttgggtgaa
     1741 gcagctgctc agctgattgg ttacgtggga gatatcacag cagaagatat tgataagaat
     1801 ccagaattaa gcagtaatgg aaaaatcggt cgctcaggtt tggagatggc ttttgataag
     1861 gatcttcgtg ggacaacagg tgggaaatta agcattacag atgcagacgg tgtagagaaa
     1921 aaagttctga tcgaacatga agttcaaaat ggcaaagata tcaaattgac aatcgatgcg
     1981 aaggcacaaa aaatagcttt tgacagtcta ggaggaaaag ctgcatcaac tgttgcgaca
     2041 acgccaaaaa ccggtgatct tcttgcgctt gctagctctc caagctatga tccaaacaaa
     2101 atgacaaacg ggatctcaca agaagattac aaagcttatg aagaaaatcc tgaacatcca
     2161 ttcatcagcc gatttgcgac aggttatgct cctggatcta cgtttaaaat gattacagca
     2221 gcaatcggtc tcgacaacgg cactatcgat ccaaatgaag tgttgacgat caacgggctt
     2281 aaatggcaaa aagacagttc ttggggatct tttaaaacaa cgcgtgtcag tagtgatgta
     2341 tcacaagtag acttaaaaac tgctttgatc tattccgata atatatatgc ggcacaagaa
     2401 acgttgaaaa tgggtgagaa aaaatttcgt acaggcttag ataaattcat ttttggtgaa
     2461 gaccttgatt tgccaattag tatgaatcca gcacaaattt ctaatgaaga tagctttaac
     2521 tcagatatct tgctagctga tactggatat ggacagggcg aacttctaat tactcctatc
     2581 cagcaagcag caatgtattc tgtttttacc aacaatggca cacttgtcta tcctaaattg
     2641 attgcagatg ataaagagac aaaagataag aagaatgtca tcagcgaaac agcagtacaa
     2701 acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc acattctctt
     2761 tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa agaaaaacag
     2821 gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa ccaaggttat
     2881 atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa acgagcatcg
     2941 gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta agtacagtaa
     3001 aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc acgatgtctc
     3061 tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa aatatttgag
     3121 aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg aatagtactg
     3181 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
     3241 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
     3301 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
     3361 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
     3421 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
     3481 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
     3541 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
     3601 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
     3661 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
     3721 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
     3781 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
     3841 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
     3901 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
     3961 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
     4021 actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa agtcatggac
     4081 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
     4141 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     4201 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     4261 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     4321 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     4381 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     4441 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     4501 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     4561 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     4621 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     4681 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     4741 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     4801 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     4861 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     4921 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     4981 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     5041 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     5101 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     5161 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     5221 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     5281 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     5341 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt tcaaattatt
     5401 tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa aaacaaaaag
     5461 gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt tacaatcttc
     5521 atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc tcctgaaccc
     5581 attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc gaaaacgccg
     5641 gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt catttcgatc
     5701 gttctatcta gccaatccgt attagggagt aagccgacta gaatgaatac tcctgaaaca
     5761 gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc tacctgattc
     5821 gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc aagagagact
     5881 agtttatcct gtaagatttg atctgctttt aattctggta agaattctag aacatataca
     5941 tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc accaccgata
     6001 acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata agcaattcct
     6061 ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc acctgtcgcg
     6121 atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt aataaaatgt
     6181 tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg gtaactacgc
     6241 acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt accaatcata
     6301 ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac aagtcctgta
     6361 cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc accgccaatc
     6421 actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg gccagtgatt
     6481 ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc atttaaaaac
     6541 acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat cccaccttca
     6601 atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc ctgtacaacg
     6661 tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt ttgtgttatt
     6721 tttttgattt taagactcaa agattcactg attttcggtg cccgtccact aatttgtaac
     6781 atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa aacgattcca
     6841 ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc tcttttttcc
     6901 caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag tttttgacta
     6961 ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag tgctaaatat
     7021 tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat ctttccaaca
     7081 agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg acaaacttca
     7141 cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc actagcgtca
     7201 cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg atcaataata
     7261 aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat acgagaaatc
     7321 tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc tgatgtatca
     7381 tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc aacacctaaa
     7441 gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca aacaaaagta
     7501 aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt ttcactgact
     7561 tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt tttcccaatc
     7621 aatgacatag taaaacctcc aataataatt atttatttat aattattgta atttagttgg
     7681 agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa cgaaggttaa
     7741 ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg ggataacgtt
     7801 gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt tgaaatcgag
     7861 cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt tccatgatcg
     7921 tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct aaatgggtat
     7981 tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa caagcttcat
     8041 gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact gctttttctt
     8101 gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg gaacaacatt
     8161 caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct gcagaaacaa
     8221 gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt tcttgctgac
     8281 gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt tcctccagtg
     8341 ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt ttacgaatcg
     8401 cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa tcatttctat
     8461 gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt aactagtaaa
     8521 tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa aattcacttg
     8581 aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa acaactggag
     8641 gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg accgtgcatc
     8701 attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg gaaatcttta
     8761 tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat tgtccactgg
     8821 tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc aagattttga
     8881 tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac gtatattagc
     8941 tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg ataagatgat
     9001 tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg aaaaaggtta
     9061 tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata taaaaaatgg
     9121 tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata aacgtttttt
     9181 atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa acggtagtca
     9241 attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt atgtacaaaa
     9301 tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga cgatgattga
     9361 tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag agggcttttc
     9421 aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg tcaatacaga
     9481 ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga aattcgaaaa
     9541 agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga tgaaggtgac
     9601 gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac tggggtatag
     9661 gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg aaatcgggta
     9721 caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga tctttctgga
     9781 agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc agcgaatcgc
     9841 cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa aatatatgga
     9901 aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg ctgcttggct
     9961 tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc cagacccact
    10021 attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag atacatcaca
    10081 tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg tagatcttta
    10141 ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag caggaccaaa
    10201 tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt tatcatggaa
    10261 atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga aatcagacag
    10321 attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg agaggtatgg
    10381 cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt aacactaaaa
    10441 gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc ccgtgagcag
    10501 tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat agaagtcatc
    10561 tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta ccgcttagaa
    10621 gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc aagagaaatc
    10681 gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga acctgagcgc
    10741 ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga aaaacttcga
    10801 gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt aggggtcaaa
    10861 atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg aatcgtaaag
    10921 aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa cggaatcact
    10981 ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat tttagcagaa
    11041 ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt tttagctaat
    11101 cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat gtaccgcata
    11161 ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca tggaagaatg
    11221 atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt aggtgctgtt
    11281 tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga taattagtga
    11341 aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga tacataaatc
    11401 cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga agtaacttga
    11461 ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat atattctgag
    11521 atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa aatatagatt
    11581 tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat gttgcagttc
    11641 tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc tatcttaacg
    11701 atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac aattcgcggc
    11761 tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg cacattttcc
    11821 ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat ataaaattgt
    11881 tcaatggcgg tttggacttc tttcaatacg ctatgttctt gataaagcat gaaagtagga
    11941 aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt tcgtgcgaat
    12001 gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac agtacaaagg
    12061 gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt taaaagtttg
    12121 tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg agctaaaata
    12181 ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat atctacagag
    12241 agatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac gtctttcaca
    12301 gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt atctatcata
    12361 ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat aaaagccact
    12421 acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt ccaataaata
    12481 tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct gccattatta
    12541 gaaaaggaga tagacaaacc gtactatgct aaaaatgcat taatcttttt cattttcgta
    12601 tacaaataag aggcactata gtttgtttgc tgactgaaac tgataatcga atttgtctga
    12661 cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta tcttccaaat
    12721 agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa aaaaggtttt
    12781 tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc taattctttg
    12841 atgtatagta aaaaatttga ttcagacata tggaa
//
LOCUS       DAYYBH010000073        12747 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000073 DAYYBH010000000
VERSION     DAYYBH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.076"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHAUG_002269"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHAUG_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8481342.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACHAUG_002270"
     CDS             complement(323..892)
                     /locus_tag="ACHAUG_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8481343.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACHAUG_002271"
     CDS             1078..1980
                     /locus_tag="ACHAUG_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8481344.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHAUG_002272"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHAUG_002272"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8481345.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHAUG_002273"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHAUG_002273"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8481346.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACHAUG_002274"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACHAUG_002274"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8481347.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACHAUG_002275"
     CDS             8554..8739
                     /locus_tag="ACHAUG_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8481348.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACHAUG_002276"
     CDS             complement(8801..9997)
                     /locus_tag="ACHAUG_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8481349.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACHAUG_002277"
     CDS             complement(10167..11603)
                     /locus_tag="ACHAUG_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8481350.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACHAUG_002278"
     CDS             11896..12705
                     /locus_tag="ACHAUG_002278"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8481351.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAYYBH010000074        12493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000074 DAYYBH010000000
VERSION     DAYYBH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.077"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACHAUG_002279"
     CDS             <1..375
                     /locus_tag="ACHAUG_002279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481352.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACHAUG_002280"
     CDS             complement(324..893)
                     /locus_tag="ACHAUG_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8481353.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACHAUG_002281"
     CDS             complement(967..2256)
                     /locus_tag="ACHAUG_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481354.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACHAUG_002282"
     CDS             2514..3458
                     /locus_tag="ACHAUG_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8481355.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACHAUG_002283"
     CDS             3472..4545
                     /locus_tag="ACHAUG_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8481356.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACHAUG_002284"
     CDS             4535..6706
                     /locus_tag="ACHAUG_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFM8481357.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACHAUG_002285"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACHAUG_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8481358.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACHAUG_002286"
     CDS             complement(9009..11195)
                     /locus_tag="ACHAUG_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8481359.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACHAUG_002287"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACHAUG_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8481360.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACHAUG_002288"
     CDS             complement(11417..11857)
                     /locus_tag="ACHAUG_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8481361.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACHAUG_002289"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACHAUG_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2631 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aac
//
LOCUS       DAYYBH010000075        12343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000075 DAYYBH010000000
VERSION     DAYYBH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.078"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACHAUG_002290"
     CDS             <1..154
                     /locus_tag="ACHAUG_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8481362.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACHAUG_002291"
     CDS             complement(208..486)
                     /locus_tag="ACHAUG_002291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481363.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACHAUG_002292"
     CDS             complement(601..1218)
                     /locus_tag="ACHAUG_002292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481364.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACHAUG_002293"
     CDS             complement(1613..2437)
                     /locus_tag="ACHAUG_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8481365.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACHAUG_002294"
     CDS             2780..3196
                     /locus_tag="ACHAUG_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8481366.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACHAUG_002295"
     CDS             3193..3663
                     /locus_tag="ACHAUG_002295"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8481367.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACHAUG_002296"
     CDS             3677..4450
                     /locus_tag="ACHAUG_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8481368.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACHAUG_002297"
     CDS             4464..5276
                     /locus_tag="ACHAUG_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8481369.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACHAUG_002298"
     CDS             5330..6295
                     /locus_tag="ACHAUG_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8481370.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACHAUG_002299"
     CDS             6431..9154
                     /locus_tag="ACHAUG_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8481371.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACHAUG_002300"
     CDS             9167..9799
                     /locus_tag="ACHAUG_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8481372.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACHAUG_002301"
     CDS             10007..10231
                     /locus_tag="ACHAUG_002301"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFM8481373.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACHAUG_002302"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACHAUG_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8481374.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACHAUG_002303"
     CDS             10556..10825
                     /locus_tag="ACHAUG_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8481375.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="ACHAUG_002304"
     CDS             complement(11004..12305)
                     /locus_tag="ACHAUG_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8481376.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4032 a         1999 c         2422 g         3890 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtgattca att
//
LOCUS       DAYYBH010000076        12221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000076 DAYYBH010000000
VERSION     DAYYBH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.079"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(324..1106)
                     /locus_tag="ACHAUG_002305"
     CDS             complement(324..1106)
                     /locus_tag="ACHAUG_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481377.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1171..2007)
                     /locus_tag="ACHAUG_002306"
     CDS             complement(1171..2007)
                     /locus_tag="ACHAUG_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8481378.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2025..3434)
                     /locus_tag="ACHAUG_002307"
     CDS             complement(2025..3434)
                     /locus_tag="ACHAUG_002307"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8481379.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3448..5271)
                     /locus_tag="ACHAUG_002308"
     CDS             complement(3448..5271)
                     /locus_tag="ACHAUG_002308"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8481380.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6114..6440)
                     /locus_tag="ACHAUG_002309"
     CDS             complement(6114..6440)
                     /locus_tag="ACHAUG_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481381.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6486..6848)
                     /locus_tag="ACHAUG_002310"
     CDS             complement(6486..6848)
                     /locus_tag="ACHAUG_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8481382.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6885..7099)
                     /locus_tag="ACHAUG_002311"
                     /pseudo
     CDS             complement(6885..7099)
                     /locus_tag="ACHAUG_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7227..7562
                     /locus_tag="ACHAUG_002312"
     CDS             7227..7562
                     /locus_tag="ACHAUG_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8481383.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7638..9635)
                     /locus_tag="ACHAUG_002313"
     CDS             complement(7638..9635)
                     /locus_tag="ACHAUG_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481384.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9733..10008)
                     /locus_tag="ACHAUG_002314"
     CDS             complement(9733..10008)
                     /locus_tag="ACHAUG_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481385.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10240..10974
                     /locus_tag="ACHAUG_002315"
     CDS             10240..10974
                     /locus_tag="ACHAUG_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8481386.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11013..12155
                     /locus_tag="ACHAUG_002316"
     CDS             11013..12155
                     /locus_tag="ACHAUG_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8481387.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3973 a         2430 c         1857 g         3961 t
ORIGIN      
        1 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
       61 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
      121 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
      181 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
      241 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
      301 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
      361 gttacctgtc cgttcttaaa taccacagaa taatcttttt tcccattttc aagtaaccga
      421 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
      481 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
      541 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
      601 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
      661 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
      721 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
      781 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
      841 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
      901 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
      961 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
     1021 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
     1081 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
     1141 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
     1201 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
     1261 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
     1321 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
     1381 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
     1441 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
     1501 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
     1561 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
     1621 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
     1681 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
     1741 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
     1801 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
     1861 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
     1921 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
     1981 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
     2041 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
     2101 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
     2161 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
     2221 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
     2281 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
     2341 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
     2401 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
     2461 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
     2521 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
     2581 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
     2641 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
     2701 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
     2761 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
     2821 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
     2881 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
     2941 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
     3001 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
     3061 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
     3121 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
     3181 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
     3241 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
     3301 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
     3361 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
     3421 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
     3481 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
     3541 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
     3601 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
     3661 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
     3721 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
     3781 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
     3841 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
     3901 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
     3961 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
     4021 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
     4081 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
     4141 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
     4201 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
     4261 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
     4321 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
     4381 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
     4441 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
     4501 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
     4561 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
     4621 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
     4681 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
     4741 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
     4801 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
     4861 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
     4921 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
     4981 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
     5041 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
     5101 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
     5161 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
     5221 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
     5281 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
     5341 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
     5401 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
     5461 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
     5521 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
     5581 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
     5641 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
     5701 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
     5761 atagccaatt tcaatcattc cgagatataa aggtatcgat agaatgattt gggctagtat
     5821 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt
     5881 atttcaattc caccaattat caataaaaat accaaacata tccataataa ttttttcttt
     5941 ttcatttctt ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta
     6001 atattttttt gtcacctgta ttagtattta atttatagga aactataaaa actccctagt
     6061 gaatttacac ctttgggagt tcctgtttgg acggtatgca taatacattt aaatcaattt
     6121 aacattaaca cctcagcata tggtatctcc acatatatac ggtctttctt tggatagtac
     6181 tcaatattta tgttgatagc acttccattg tctaatatag ctttagcccg gtatgtgtta
     6241 tctccatttt catgagtaat cgtcacatca tcacatgatc gatttaagtt gtactgttct
     6301 tgtaaaatct gtgttaccaa tggtttcgat gacttctcta atccagatgt acctgtcatt
     6361 tttgaaaaaa tgatacctat aatagccaag acaacgataa ctataatgat aaacttcttg
     6421 ttcatattta ctttattcaa ctttttatct cctatctcca aaataatttg ctcttatccc
     6481 tcttatcagt acattgacat ccctaaaagt acaccaaaga ttgctccagc tacccctgct
     6541 atcatcaaaa tcgtacccat ttgttcatct ttatctcgat ataagtagcc cataataaca
     6601 gcaacagcac caaatacaac aggaataaat gctaacgcta ctaccataga aatccaacca
     6661 ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta
     6721 attgtctgct cagtactttt ctttactgcg tctgctccag ttgtctcatc tgtttcttgt
     6781 agtgctccgc aaaactggca atacttactt tcatctgtta tttctttgcc acattttcta
     6841 caatacatgt cttctcctcc taaaaacatt caattgttta ttagttaact gtttacttta
     6901 ttaaatacat cagaaaacag ctcattcatt ggtacactta atatctcaga aagcatcttc
     6961 atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta
     7021 acattcattt ttttgctaac tccgtttgag aaagtccttt ttcttttctg tatcggctga
     7081 gattacttac ttttttcatg attctcccct cctgttttat tatatacaac aatatgatgt
     7141 atttcaactc ataaaatata aaaaaacttc aaataccatt acaaatacaa caacttgttg
     7201 tactatactg agatgggggt gtattaatga aacagaaaca atatgcaaag gtcattagag
     7261 aaaagcgaaa agctctcggg tggacacaaa aagaattagc tgaaaaaatc ttctctacac
     7321 aacaggcagt agcaagatgg gaaaattctg taacagagcc taacctcgat agcttaaccg
     7381 ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt gtcgtagatt
     7441 atgaggaaga gtttttggct ctctatcgtt ctcttagcac tgaagatgca gttcgaacaa
     7501 ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt
     7561 gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag
     7621 agctatggat aacctgtcta gtttaataca attgggtctt ttctcttcct tactctccgt
     7681 ccatcatctc ttgttacact tgtaaattga taaagaggaa ctaattcaac tcctgataat
     7741 atcaattctt ttattacttc atctatccct gctccttttg ctaggtagac taaaaaacta
     7801 ctacttgctt tcacattgta tagtctctta atatccttag aagctcgttc aagagtgaat
     7861 ccttgtttaa caaagtagcc tctgttttct tttaaagcat tttccacgct gttattccca
     7921 acattacgta gacgttcaca aacagtgtta gtaagttctt tctctgtcat tagatatgtt
     7981 ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc
     8041 ttgctaagtt ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaaa
     8101 catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaaattt ttccagaagt
     8161 ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac gtctaatcga
     8221 tagacattgc ttctcttatg attaaagttt cctaacgctt tctcttcctt taacctattt
     8281 aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat caatcctata
     8341 aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt accatagtaa
     8401 aaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact ttccttacta
     8461 tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat ttcctctaga
     8521 taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc aaaaagtact
     8581 tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt ctctaatagc
     8641 caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac tccaataaaa
     8701 ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc agttttactg
     8761 gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa gtttttactt
     8821 gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc attaaaagac
     8881 atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc tttgtcgtca
     8941 ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct ccctgaatca
     9001 ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt aatatctaac
     9061 atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa aaacattcta
     9121 ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta ttcgtaaaca
     9181 gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat cttgtatttt
     9241 tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt atgaactaaa
     9301 ttgatagata aaaccttttc tatgcttaag tatctactat catctttact caaatctact
     9361 accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac tctggtttcc
     9421 acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa tgattgtgat
     9481 gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc taaacaccta
     9541 tctcttacat cagttttttc tttttttgta actggcttct ctaataattt ttctttatct
     9601 acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt ataaaattct
     9661 gttaaagact aaaacataca gataataaaa acaaactctt tattactata tgttttagtc
     9721 ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa
     9781 tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat
     9841 cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat actctagagt
     9901 gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg
     9961 ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc
    10021 tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa
    10081 aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac
    10141 acataatacc tcgataaaaa agaggtgtca atatctttgt aaaatttatt tacaacaccc
    10201 ctttttcgag gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata
    10261 aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa
    10321 aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa
    10381 actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt
    10441 gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt
    10501 ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa
    10561 aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg
    10621 ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt
    10681 aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc
    10741 atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa
    10801 gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat
    10861 cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca
    10921 tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa
    10981 gtactccttg taaactaaga ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga
    11041 aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg
    11101 aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg
    11161 acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg
    11221 aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg
    11281 caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat
    11341 cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa
    11401 gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat
    11461 atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa
    11521 atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg
    11581 gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg
    11641 aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata
    11701 aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt
    11761 ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac
    11821 agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag caacatgttt
    11881 ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata
    11941 ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta
    12001 gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa
    12061 atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag
    12121 gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt
    12181 tctagtatac aggcaaaaca aaaaagctag aacccttata t
//
LOCUS       DAYYBH010000077        12200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000077 DAYYBH010000000
VERSION     DAYYBH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.080"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..649)
                     /gene="frr"
                     /locus_tag="ACHAUG_002317"
     CDS             complement(92..649)
                     /gene="frr"
                     /locus_tag="ACHAUG_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8481388.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(652..1374)
                     /gene="pyrH"
                     /locus_tag="ACHAUG_002318"
     CDS             complement(652..1374)
                     /gene="pyrH"
                     /locus_tag="ACHAUG_002318"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8481389.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1510..2391)
                     /gene="tsf"
                     /locus_tag="ACHAUG_002319"
     CDS             complement(1510..2391)
                     /gene="tsf"
                     /locus_tag="ACHAUG_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8481390.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="ACHAUG_002320"
     CDS             complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="ACHAUG_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8481391.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3631..4110
                     /locus_tag="ACHAUG_002321"
     CDS             3631..4110
                     /locus_tag="ACHAUG_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8481392.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4327..5418
                     /gene="phnW"
                     /locus_tag="ACHAUG_002322"
     CDS             4327..5418
                     /gene="phnW"
                     /locus_tag="ACHAUG_002322"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM8481393.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5411..5539
                     /locus_tag="ACHAUG_002323"
     CDS             5411..5539
                     /locus_tag="ACHAUG_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8481394.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5543..6088
                     /locus_tag="ACHAUG_002324"
     CDS             5543..6088
                     /locus_tag="ACHAUG_002324"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8481395.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6542..8233)
                     /gene="argS"
                     /locus_tag="ACHAUG_002325"
     CDS             complement(6542..8233)
                     /gene="argS"
                     /locus_tag="ACHAUG_002325"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8481396.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="ACHAUG_002326"
     CDS             complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="ACHAUG_002326"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8481397.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9715..10734)
                     /gene="argF"
                     /locus_tag="ACHAUG_002327"
     CDS             complement(9715..10734)
                     /gene="argF"
                     /locus_tag="ACHAUG_002327"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8481398.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="ACHAUG_002328"
     CDS             complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="ACHAUG_002328"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8481399.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3341 a         2529 c         2203 g         4127 t
ORIGIN      
        1 taattttttc tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg
       61 aatgatccgc ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat
      121 cagcagcgat cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt
      181 ctaatccgcg caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat
      241 cgatcgcatc acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca
      301 catctttagc taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga
      361 catttccatc attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat
      421 cttgaagaat gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga
      481 ttgatgccat ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca
      541 aaaggctagc gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg
      601 ctgctttttt cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc
      661 ccttacagtt gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt
      721 taaattgaag acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc
      781 catgacttgt aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc
      841 atttgcatct actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag
      901 gataacatct gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta
      961 cgggttgccg gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc
     1021 tttacggcga atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt
     1081 cggtacaccc acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa
     1141 catacccatg taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg
     1201 ccagatattt ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt
     1261 gatttcttgg atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc
     1321 taaagcttct ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat
     1381 tcctccaata ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact
     1441 ggtttttctt gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat
     1501 tcaacagaat tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt
     1561 ttcgatacct tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc
     1621 aacgtatttt tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat
     1681 ttctgctttg aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt
     1741 gccttcgttc aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg
     1801 aatttgtgat tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct
     1861 tgcaactgtt tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat
     1921 atgcaagtat ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa
     1981 gctgattttt tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt
     2041 atctgttttg attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc
     2101 tgcaatttct tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga
     2161 gtttacttca acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc
     2221 tgcaatgcgg tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac
     2281 tgctttttcc atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac
     2341 gccagtcatt tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc
     2401 ctcctgtgtg tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc
     2461 agcctttgaa acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca
     2521 acatctacga tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact
     2581 acttgatctt caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat
     2641 ttaacggcac gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg
     2701 cagtttgtat caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca
     2761 atacgttctt tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg
     2821 ataccgccta agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt
     2881 ttaggtagaa cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg
     2941 atacgttttt ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg
     3001 aagtattgtc ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta
     3061 cctacgaata atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca
     3121 tctactaatt taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag
     3181 atatatttct tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct
     3241 tctagtaatt gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt
     3301 ttcctcttct tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa
     3361 taagaatgtg gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc
     3421 gtatcgcaag agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa
     3481 aagaattcaa gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa
     3541 taattttagg tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac
     3601 atgtgaaatc tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt
     3661 aaacaagtca ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg
     3721 cattatggcg ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc
     3781 gtgaaggcac ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact
     3841 cctgagacta ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg
     3901 aaagtcgaac gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta
     3961 tttgcatctg ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc
     4021 gatacgatca tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt
     4081 caccatccaa ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg
     4141 tgttttttta ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata
     4201 catcattttt tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata
     4261 ttggatgtta acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga
     4321 aatcatatgt ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat
     4381 gctatgctga tcgatcattg tacttgggat caagaataca aagaaattac acaatggata
     4441 agaaaaaaat tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg
     4501 caaggtagca gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat
     4561 actgtactga tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt
     4621 ctgaaactga actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg
     4681 attacacagc tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag
     4741 actacttccg gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat
     4801 cttttgttta tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa
     4861 ctagggattg attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt
     4921 tcttttgtac ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg
     4981 tcacttgatc tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc
     5041 acttccccta cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa
     5101 ggaggaatcg aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa
     5161 atgacagaga tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct
     5221 tttctttacc ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa
     5281 aatggatttg tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat
     5341 attggcgaga tctatccgga agatattttg gaactgacag caattatcaa agactataag
     5401 gagaacgtta atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg
     5461 ctgtatggct cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa cccaactgac
     5521 tgacaaagaa atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct
     5581 ttccctccct tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa
     5641 agaggtggag gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca
     5701 ttctaccttg aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa
     5761 tattggttcc acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa
     5821 aaaagaaagg tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg
     5881 accgtttcct tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga
     5941 agtaatcaaa gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt
     6001 gacagtaggt gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa
     6061 attagagatg gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc
     6121 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6181 aagaatccag aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa
     6241 gtaataaaaa cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6301 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga
     6361 cttttgtcac agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta
     6421 aacggtgttc attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc
     6481 tattttctct tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt
     6541 cttacatctt atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag
     6601 tcactgcata aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg
     6661 tatgagcata gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg
     6721 acggttcgta tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa
     6781 ccacttccca gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc
     6841 ctgctttttc caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt
     6901 ctccttcaaa tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt
     6961 tcaaatcgtg gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt
     7021 ccaaatcagg gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata
     7081 cttcttctaa taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga
     7141 tcaaaccaaa tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga
     7201 cagctttcaa ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt
     7261 tgaagtcgta agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg
     7321 tcgcaccgtc tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga
     7381 tctctgcacc tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat
     7441 ccatcttgtc attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat
     7501 catagatttt attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag
     7561 cctctttatc gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg
     7621 gttctgtttc agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt
     7681 ctgctttaac agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact
     7741 gtgttcccca gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa
     7801 tgaaaccgat cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg
     7861 caatattagg agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc
     7921 cataatgttc tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat
     7981 tcatgaaaaa gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga
     8041 ttttttctgc taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg
     8101 aaaaggctgg aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct
     8161 cgacttgttc cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat
     8221 ctttattatt catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc
     8281 tctagcgacg gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc
     8341 caattgtcct aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa
     8401 cagaattctc caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat
     8461 tcgctagcca agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact
     8521 ttcttgaatt atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaaaaatcca
     8581 gatggcctaa aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa
     8641 agacttaaca aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc
     8701 gatattttct aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat
     8761 agctgcttct actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg
     8821 tttcatttct gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac
     8881 atacacattg tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa
     8941 tttttctgaa gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt
     9001 ttctacaaca ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag
     9061 cgtttcgatg acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct
     9121 ccatccacgt ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt
     9181 ggcttcttct tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc
     9241 attttcatcc acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc
     9301 cttcagtact tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc
     9361 aacacatgta tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat
     9421 caataggtta ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc
     9481 gataaatttc actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact
     9541 tgcatccgta gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat
     9601 ttcattcgtc ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt
     9661 tgtgattttc cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac
     9721 gcgagggata aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg
     9781 attttctgct tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc
     9841 catttcagtg atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg
     9901 gaaagctggc aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat
     9961 ttccatattg atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc
    10021 acccattgat acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac
    10081 atcgtcggta atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac
    10141 gatttcttct tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat
    10201 tgcacctgtg actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac
    10261 agtgatacct tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg
    10321 tgttggatgc cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag
    10381 ttcctctacc attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag
    10441 tactttagct gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag
    10501 atattcagga tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt
    10561 agatgttttt tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc
    10621 acgttttttc aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt
    10681 tgtaaagtct ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa
    10741 cattctcctt tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca
    10801 gtaagactgc cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat
    10861 gcagcgtgga ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat
    10921 gccatagcta cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc
    10981 tggagcgatt gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc
    11041 atctccatta ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt
    11101 ttcgtgtaag ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa
    11161 gacatccatc ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc
    11221 taccattgta aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc
    11281 caacactttt tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc
    11341 tttcgcatta gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc
    11401 gtctccgcct tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt
    11461 tgcaaatctt ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc
    11521 ttcaaaagtc atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt
    11581 aaagtacaag tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc
    11641 atatagactg ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc
    11701 taccatttct tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt
    11761 cgatgcaata tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc
    11821 gtcaatcgct tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa
    11881 tgtcttcgca aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa
    11941 caacagacga tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag
    12001 taaaactgtc tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg
    12061 ttgcctcctt ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa
    12121 ttttgtctca ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa
    12181 cgtttttcta tttttaagcg
//
LOCUS       DAYYBH010000078        12183 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000078 DAYYBH010000000
VERSION     DAYYBH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.081"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..309)
                     /locus_tag="ACHAUG_002329"
     CDS             complement(145..309)
                     /locus_tag="ACHAUG_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481400.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            604..1932
                     /locus_tag="ACHAUG_002330"
     CDS             604..1932
                     /locus_tag="ACHAUG_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8481401.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTVVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2134..2967
                     /locus_tag="ACHAUG_002331"
     CDS             2134..2967
                     /locus_tag="ACHAUG_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8481402.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2983..3720
                     /locus_tag="ACHAUG_002332"
     CDS             2983..3720
                     /locus_tag="ACHAUG_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8481403.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3875..4843)
                     /locus_tag="ACHAUG_002333"
     CDS             complement(3875..4843)
                     /locus_tag="ACHAUG_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8481404.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5075..5908
                     /locus_tag="ACHAUG_002334"
     CDS             5075..5908
                     /locus_tag="ACHAUG_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8481405.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5942..6781
                     /locus_tag="ACHAUG_002335"
     CDS             5942..6781
                     /locus_tag="ACHAUG_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8481406.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6807..7367
                     /locus_tag="ACHAUG_002336"
     CDS             6807..7367
                     /locus_tag="ACHAUG_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8481407.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7566..9287
                     /locus_tag="ACHAUG_002337"
     CDS             7566..9287
                     /locus_tag="ACHAUG_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8481408.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9452..10594
                     /locus_tag="ACHAUG_002338"
     CDS             9452..10594
                     /locus_tag="ACHAUG_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8481409.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10610..11821
                     /gene="thiI"
                     /locus_tag="ACHAUG_002339"
     CDS             10610..11821
                     /gene="thiI"
                     /locus_tag="ACHAUG_002339"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8481410.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4152 a         2044 c         2530 g         3457 t
ORIGIN      
        1 gccggagtaa ataaagctta tgcaaagaaa aagaaatcca atcacttggg tttctttttc
       61 ttttattatt atgatgaatc aagctttctg tttttgtcac gtatgttgta atctttacga
      121 aagagtagag ttggctactc ataatcaacc atacttccca tcatcgctaa atacgaaata
      181 aatcagcatt cctacaccaa tcaaagcaca agctgcacca agaaaaggga tgacattccc
      241 aaagttatct gtattcataa gtatgccacc acccacaaac aaaaagccgc cagcaatcag
      301 gtttttcatt ttattcatct cctttcttac gttatagtat ttccttcttt caatgagtat
      361 tttgaaagct atcttattgt atgatgttct tctaggcctc ttctttatta tacaggccat
      421 tttcgttatc tcatagaaat attccttttt atctgaaaac tattgtatac cgtatgacat
      481 ttttcttcat gaagttgtaa gtatattctc attcaaatga aaatttcatc atagaagaga
      541 ttgtatttct aaattcttaa gagtaaaata cgaagtataa cttggatttg gaaggatgat
      601 tggatgaaag ctattacgcc tgatatgaca acaaaattta acaaacagtt tcaagcaaat
      661 atcaaacagc aggcattgca gcgggcagtt gtgaagaacg gaattactgc ttctgctgaa
      721 aatgtttcag cacatgtgga gaatacaccg gtattttcta tagatttggc tacggggaaa
      781 gtggctaatc aaatgcaatc ggggcgttgc tggatgtttg ctgcactgaa cacattccgt
      841 cataagatgc tgaatcaatt ccatttaaaa gagtttgaat tatcacaaaa ttacactttt
      901 ttctgggata aatatgaaaa ggcaaattat ttctatgaaa acatcattgc aacaggaaaa
      961 gaggcaatcg atagccggaa agtagctttc ttactcgcta ctcctcaaca agatggcggc
     1021 caatgggata tgatcgtttc attattccaa aaatatggcg ttgtgcctaa atcggtgatg
     1081 ccggaaagtg cgaatagttc aaattctcga gatttgaata actacttgaa caaattactg
     1141 cgtaaagatg cagtagtgtt acggaaaatg gttgctcaag gagaaacatt agacgctatt
     1201 gaagaaaaga aagaagaaat gctagaaagc atttataact tcttagctat ctcattagga
     1261 acacctccaa aagaattcga ttttgagtac cgcgatgaag aaaagaacta tcatcttgac
     1321 cgaggactga caccacaaat tttttatgat aaatacatcg gtgtaaaatt agatgattat
     1381 gttagcgtga tcaatgcacc aacaaaagac aaaccattca atcgttctta tacagtagag
     1441 atgctaggaa atgtcgttgg cgggaaagaa gtcaaatatc tgaatgttga tatggaaact
     1501 ttcaaaaaat tagcgattgc tcagcttgaa gaaggagaat ctgtttggtt tggttgtgat
     1561 gtaggtcaat cttctactag aactagtggc atcatggcct tagatgctta ttcaatggat
     1621 gatctatttg atacggattt tacaatgact aaagctgaac gtttagattt tggagaaagt
     1681 ctgatgaccc atgcgatggt attgaccgtt gttgacttag tagacgggca atcaacaaaa
     1741 tggaaagtcg aaaatagctg gggcgaaaaa gttggaaaga atggcttctt tgttatgagt
     1801 gatgcctgga tggacgaata tacgtaccaa atcgttgtaa gaaaagattt attgacgaaa
     1861 gagcaattaa atgcgttcaa tgaagaacca attgttctat ctccgtggga tccaatgggt
     1921 gctttagcat agacgagtat ttcaatgaag agtatgtagt aaagataaaa agagctggct
     1981 taagccagct ctttttatct ttaaagtcca ttccctgttt tttatggtcc ctgtgctata
     2041 attctaaata aaggataagg attggaaata atccttttat cattaaaaat atgatgacga
     2101 agtagaataa aattgggagt aggggggaaa gtcatggata tccaaactgt tttatctgat
     2161 ctgaaattaa atggtaaagt gattccagtt gttggtggcg atgttaatca gacctatcgt
     2221 ataaaaaccg aacatcgtgc ttatttttta aaaatccatc cgaatgtaaa aaaaggattt
     2281 tttgaagcag aagtagacgg attaaaagaa ctgtcagcat tcgtacgtgt accggatacg
     2341 tatatgctag gtgaaacaag tgaaggcgct tatttgttaa tggaatggat cgagccggga
     2401 aaaggggatc aacgagatct tgctgcagct ttagctaatc tccatcagca aacagcgcca
     2461 caatttggtt ttcgtaaaga taactatttg ggtacacttg ttcagaaaaa ttcttttgaa
     2521 gaagattggt ggactttttt ctttaaagat cggcttgaat cccagatttc tttggcagaa
     2581 gaaacgaatc gatggaatgt acagcgtcaa gaaaagtatc tgcgatttaa agaacgtgta
     2641 ttgaaatcgg ttgaaccaaa aaagatcact ccacgcctct tgcacggaga cctgtggagt
     2701 ggtaatgtgt tctttgacca gcaagggcat ccagtttttg tcgacccagc tgtttcttat
     2761 gggaatcggg aacaagatat tgcaatgagc cagttattcg gtggtttccg accggaattt
     2821 ttagatgctt atcaaactat atttccatta gaaaagggat ggaaagaccg actgccgatt
     2881 taccagttat attatctttt ggcacatttg aatatgtttg gagaaagtta cggttcccaa
     2941 gtagatcaat tactagaaaa tttttaagag gagggcgtgt ttttggcaaa aaaatatacc
     3001 aaaaagcatg aagtttcata ttatgagtgt gacatcaacc aaacgatgac atttccttcg
     3061 atgttaggag tagttatcaa aacgtctgaa gatcaaagcg accagttggg aagaggcagt
     3121 gaattcgtca atagctttgg acttacttgg gtcatcacga attattccat ggagattaat
     3181 cgtctcccta gagtcggtga agtaatctca gtaacgactc aagccatgga gtacaataag
     3241 tttttctgtt atcggaactt ctggatccat gatgaagatg ggaaagaatt agtcaaaata
     3301 gaatctgttt tcgtattgat ggattttgtg aatcgaaaaa tgagcagtgt taatgaagag
     3361 atcattgctc cttttgagag tgagaagatc aagaaaatca agcgtcagga aaaaattgaa
     3421 aagattgatt cgggcttcat gttgccatat cgtgttcgtt tttatgatat cgatagtaat
     3481 cagcatgtta ataatgcgat gtattttaat tggatcattg acgtattagg atatgacttt
     3541 ttgactacgc atgttccaga aaaagtaatt attcgatttg ataaagaagt agagtacggg
     3601 aatgagatcg aaagccattt tgaacaaatc cctatcgaaa atggtgtcaa aacaaaacat
     3661 gaaatccgaa tgcaagaaca gctgtattgt gaagcaaata ttatatggaa aaataaataa
     3721 aaaagaggtt gtgacagaag ggggcaactt caagaaataa gaagaaattt acgaaaattg
     3781 tttctcaaat ttttgtaaat tttcgactta gttttcgaag aagctatttt tgtcacagcc
     3841 tctcttttta agtttagcta ttagataatg atgtttagtt ttggataaac gaaatcaatt
     3901 cttcacccga caaaccatag tttacagcaa tcaatagttt taaacgtgct ttttgactat
     3961 taatactgtt acagaaaata atccccattt tttctagttc ttttcccccg ccttcataat
     4021 cataaacagg ctctgcgatt ccattgaaac aacgagaaac aagtactaca gggatttttt
     4081 ggacaagaag cttttccaaa gcagctaagg cttgtggagg gagattcccc gcacctaaag
     4141 cttcgattac caaaccgtct actttcgtat gggcgatggc ttccaataag tccccttgca
     4201 tcccagcata tgctttaacg atcgggatag tcccatctac agcggaaatg tccagtcgct
     4261 ttgtttctaa aagttcctgc aagaataaaa ttcgattctt agttactaag ccgatgggac
     4321 ccaatgtagg tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc acgtagcgtg
     4381 cagaatggat ctcatcgttc atgaccacca aaaccccctt atctagcgct tcttcacaac
     4441 ttgcgacacg aatcgcactt tcaaaattat aaaggccgtc acttccaagt tcattacttg
     4501 aacgcattgc cccagtcaaa acaattggta atttgttgcc aatcgttgta tcaagaaaaa
     4561 aagcagtttc ttctaatgta tctgtaccgt gtgtgatcac cactccggat atacctgatg
     4621 cataagcgga caagatacgt tcttttaata ccaacatgtg agaaggcatg atgtgtggtg
     4681 agggaagatt gaagatatct tcaactaata attctacatg cttggggatt gtgatttctg
     4741 catctaataa tgggtttgcc acatcaggac taacaccgcc cgtgagcatg ttttctttca
     4801 tggcgatcgt tccgccggtg tgtagtacaa gtattttttt cattttacag acttccttac
     4861 gaataatttt gcatagccgt taaatcttat tgtatactat ataaaagagt tttgctatca
     4921 gtgaagaata acgaatagcc taacttatca taaaaaagct gaccttatta aaatggaaaa
     4981 gactcaacct tgacctccta atacaatgga gtatctggtt gagtttcttt tacaagaaag
     5041 cagccaagga aatcaatcac gaaggtggcc ggaaatgatc agaatcggat taacatcgtt
     5101 taaagaacat gacaaattaa ctggtaaaaa gaataacact ctttatgaat atgcagggtt
     5161 tctaccctta gtggaattag atactgccta ttatggaatt ccccgaaagt cctcagttga
     5221 gaattggata agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct atcaaggaat
     5281 cagcggtcaa ggagactgga agccttatta tcagaccgaa gaagagatga ttgcgcattt
     5341 tttagatgtt atgtcgccaa tggtagatgc tggaaagatg ttttgctttt tagtccaatt
     5401 cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac ttagaaagtg
     5461 gtttgagggt tatcctgttt ctatagaatt gcgggattat tcttggtaca gcgatgaatt
     5521 tcttgaacgt acaagaaact ttatgtctag agccgctttt tctttagcca tcatcgatga
     5581 gccacagttg ctgaatacaa cgattccttt tgatccattt gtcaccaatc cgaactttgc
     5641 tctttttcga ttccatggta ggaatgcgca aggatggcaa gcaaatgata aagagtggcg
     5701 taaaaaaaga acgttatatc gttattcaga aaaagaactg caagaactag cacatgaagt
     5761 caaagtagtt gattctaaga caaaagaggt aggtgtgatt tttaataata attctggtgg
     5821 tgatgcggct gaaaatgcat tagcactgca aaaaatatta aatatcaagc cagatggact
     5881 caatccaacc caaatggatc tgttttaagg gcttatttta ttcaagaaag gggcgaagtc
     5941 tatgatcaaa gctatttttt ttgatattga cggaacattg gtcaccaatc gttcaaaagc
     6001 gttggaatca acaaaacaag caattgaata tgctcggaaa aacggcattt tatgcggtgt
     6061 agcaacgggg cgcagcccag taaagatcaa agaaatcatt gatgaattgg aattagatat
     6121 gtatgtcgtg tataatggtc aactggtttt tactgcagat cgcacgatta tcgaccaccc
     6181 ttttgaacaa aaagtattgg aacatattgt tgaatttgca gatgagaacc atcgacaaat
     6241 cgtttttggt gcgagaaatc ggttagatgg atcaacgacg atgctattag gacaatcgat
     6301 ttttatcaag cgactagtca gctttttacc gcgtaaattc ccggtacgtc tgatgaaaaa
     6361 aatcctccag gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt tagagatatt
     6421 aaaagaacca atttatcaat gtatattgct cagtccggag tcagaacaga aaaagttaga
     6481 acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg tatactgtag atatcatccc
     6541 aaaaggtggt tcaaaattac taggcatcca agctttcgca aatgctgtcg aaattgaaat
     6601 ggaagaaatt atggcattcg gcgatcatta caatgatatc gagatgctga aaggtgtagg
     6661 aattggagtg gccatgggga atgctcagat cgaagtaaaa catgctgcag actttgtgac
     6721 tcaatcaaat gaagaagatg ggatatacga aggactgaaa cacttcggtc tgatttatta
     6781 gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac accttttcaa aaagtagaaa
     6841 cattccacaa aacttttgat ccaagaagac cagagatacc cacggctttt acagaaaatg
     6901 aagctcatac acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt gcagctactt
     6961 cacctaataa agaacaactg catgaagctg ttatgctgct gcacgaagca ttagataaag
     7021 cagaagaaaa aatacttggt aaaacacagt ggggaagtga tccgctggtt gaacaagtag
     7081 atgcactgac agatatcctt tactttactt atggttcttt ttcattacta ggaatagtgc
     7141 ctgatcgaat attcgaaatc gtccatcagg caaatatggg gaaattattt ccagatggaa
     7201 aacctcatta tgaccctgta acaaataaag tgttgaaacc tgaaaactgg cagatggaat
     7261 atgctccaga acctaaaatc gcggcagaaa tccaacgtca gattcaagaa gcgattgcta
     7321 agaagaaatc cgctgatata ggcatgtgca caaatgaaaa agagtgatca gagtttcact
     7381 atttttgtta tctttatacg atggaaaaac aaaagatttt cgtttaagtt agtgaatgca
     7441 tgaaaatagt atgctataat ctagaataga tgtcttttgc acgagagacg tattatttgg
     7501 atttatgcca agcagaacgc catctgttgc ataaaagatg catactgaaa acggaggaac
     7561 cataaatggg aaataatgtt atcatcggaa tcgtcattgc aatcattata gtcgccattg
     7621 tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca agatcgattg gatgccttag
     7681 aaaaacgcaa ggaagaatta tttgacttgc cagtgattga agaagtcgat gatgttaaaa
     7741 aaatgcatct ggttgggcaa agccaaaata cctttcgtga atggagtcaa aaatggaatg
     7801 acatatctac ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg gaaaacctca
     7861 atgaaagttt tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa gctacaatgg
     7921 accaaatgga gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg cgggaaacgg
     7981 aagaacgcaa ctcactagct gttcagcagg cacttgacgt ctatgaagaa ctgaaagaaa
     8041 aagtcagaga agacgagcat gaatacggac cagctctttt tgaattgcaa aaacagatca
     8101 aagtgatcga aaaagaattt acgcagtttg tgactttaaa cacttctgga gaccctgttg
     8161 aagcccgcga ggttttggat aaagcagaaa aacatacgta tgaagtggaa gctctgatga
     8221 aaaaaattcc accgctgtat gaggagctcc ataccacttt ccctgaacaa cttgaagaaa
     8281 tcagtgatac ttacgagaaa ttgatggaag aacaatatgt attcccagaa gaagatcttg
     8341 ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc attagccaat cttgaaaaaa
     8401 cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt gattcgttat
     8461 atgatatctt agaacgggaa atggaagctc agagatatgt aaaaactaac caatcaacga
     8521 ttgcagagta tatcaaacat acaacaaaga ataaccgtca attattgatt gagctggatc
     8581 acacatctca aagctataca ttgaaccaca atgaaattgg ccgtgtacga ggtttccaaa
     8641 ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat tccacagatc cgtcaacacg
     8701 aaattcctta ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc ttagaagata
     8761 tcgaaaagca acaagtagag atcgacgact cattacatga attgagaaaa ggcgaaaaag
     8821 aagcgcaaga aaagatcgat acattcgaat tcaaattgcg cagtttgaaa cgttttgtag
     8881 aaaaacaacg tttgcctggc ttgccgaatg attacctaga attctttttt gttgctactg
     8941 accgtattga agaactaagt gttgtcttga ataaaattcg agtaaacatg gaagaggtca
     9001 atcgtttagt tgctctatgt gaagaagatc tagaattatt agataagaag acacatgatt
     9061 tagtagatgc agcagcatta acggagcaaa tgctccaata tgcaaatcgt tatcgtcata
     9121 cacacgaaga agtgagagaa gcaattgata aaagctacta tttattcaat aaagaatatc
     9181 actatcaaga agcattagac gaaatcggta cagcattaga gcgagtagaa cctggtgctt
     9241 tcaaacgcat cgaagatttt tattttaatc atccagactt agtataaagc aaaaaagtta
     9301 gtaaaccagc aagagcaaga cggattattg tcttgttctt gtcttttttt gtatatttga
     9361 aaattgaaac aagtgaaaat ttcacctgct cttgaaagca ccttggacaa ccgttataat
     9421 agaaaagaat ttcaaaaaag aggggtttct tatgatatat tttgataaca gtgcaacaac
     9481 cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga ttattgggaa
     9541 tccatcaagc ttgcatgatt taggcaatca agctaaccgg ttgttgcagc aagcaagaaa
     9601 gcagatcgct gatttgatcc atgtagaacc agaagaaatc tattttactt caggcggaac
     9661 agaaggagat aactgggtat taaaaggaac gatgatcgaa aaaagagaat atgggaatca
     9721 tatgatcatt tcaggagtcg aacatccggc cgtcagcgag acagcagaac aattaaaaga
     9781 attaggaatc gaagtaagta tcgccccagt ggataaacgc ggatttgtac gtgtcgacga
     9841 attgaaagaa ttaataagaa aagagacagt attagtctct gttatggctg tgaataatga
     9901 agtaggatcg attcagccca tccaggaaat cagtgatctt ttggatgatt ttcctaaaat
     9961 tcatttccat gtagatgctg ttcaggcgat tggaaaagta ccattctcgc aatggttgac
    10021 agaaagagtt gattttgcaa cattttcagc tcataaattc catggcccac ggggaactgg
    10081 tttcatttat tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg gagggcaaga
    10141 aagaaatcag cgaagtggga cagaaaatgt tccaggtatt gtggctatgg ttaaagcttt
    10201 acgtttatca ttagagataa aggaacaaaa acctgagcag acacaacgct taaaagtagc
    10261 tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag gaactgacta
    10321 tgcaccgcat attttatgtt ttgctctaaa aggaattcgc ggcgaagttc tcgtccatgc
    10381 cttagaagaa aaacaaatat ttgtttctac tactagtgcc tgctcctcac gtaaacatat
    10441 ggcaagcagt acattgcacg caatgcatgt accaaatgat ctggctacat ctgctgttcg
    10501 aatcagctta gatgaaaaca attcgcttgc agaagtcgag caatttatga ttattttcaa
    10561 gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg tgaaatacag
    10621 tgaaattatg gtccgttatg gggaactttc gacaaaagga aagaatcgaa aaagctttat
    10681 catgcaacta gcacaaaatg tcagacaagc tttgaaagac tttccagaaa tcaaaatcca
    10741 tgctgatcgg gaccggatgc atcttttatt gaatggcgca gacgggcagc tagttattcc
    10801 aaaactagca aaaatatttg ggatccaaaa tttctctcca agtattcgtg tagaaaaaga
    10861 tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa gaaatttata ctgggaaaga
    10921 aacatttaaa attatcgcta aaagaagcga tcaccaattt gaattgacaa gtaatgaatt
    10981 gaatcaaaca ttaggtaatg ctgtttttga tattttcccg catatccaag ttcaaatgcg
    11041 tcaaccagat attccattac gtgtagagat tcggcgtgat ggcgcttatc tatcgtatga
    11101 aacgatcaag ggagctggag gattgcccgt aggaacaagc ggaaaaggaa tgcttatgct
    11161 ttctgggggg attgattctc ctgtagcagg gtactttgcc atgaagcggg gagtggaaat
    11221 tgaagctgtt cattttgcta gtccgccgta tacaagtgaa caagcactgc aaaaagcaaa
    11281 agatctaaca gccaaactag caccttatgt gggaacgatc cattttatcg aagtgccatt
    11341 taccgagatt caagaagaaa tcaaaaagtc agtgccacaa ggatattgga tgacaattac
    11401 tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt attcgtcatg gtttagtcat
    11461 tttgaatggt gaatcattag gtcaagtggc ttcacaaact ttgcaaagta tggtggctat
    11521 caatgaagta acgaatacgc caatcattcg gcctgtagcg acaatggata aacttgaaat
    11581 cattgaagta gcagaacaga tcgatacatt taatctttct atccagcctt ttgaagattg
    11641 ctgtacgatt tttgctcctc cacagccaaa aacacgccct caactggaaa aggtccatca
    11701 atatgaagag cgtttagcaa tagatgaaat gatccaacga gcactagctg gcttgaagat
    11761 ggaagagata caagcagaat cgtctaatga aaaaactgaa gaattttcag aattgctata
    11821 agaggttatg aaaatagtgt tttgaccaga gtattagaga aacaaaacga aaaaatagct
    11881 ccatgtattt taccgttttt gaactaatgt cgaagattag ctgattgaat accatttatc
    11941 aagaagttat ggaaaagccg gttctttttt actaactgaa aaataaaatt ttatttagag
    12001 atcaagctac agttgataaa tgatataaaa aagagaattt cccatgaaat agcgaggttg
    12061 tgacagaagt gggcagcttc aagaaataag aaggaattta cgaaaattgc ttttcaaatt
    12121 tttgtgaaat ttcggcttat ttttcgaaga agttacttct ggaacaccgt gtatcagagg
    12181 ttg
//
LOCUS       DAYYBH010000079        12159 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000079 DAYYBH010000000
VERSION     DAYYBH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.082"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..406
                     /locus_tag="ACHAUG_002340"
     CDS             212..406
                     /locus_tag="ACHAUG_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481411.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            403..897
                     /locus_tag="ACHAUG_002341"
     CDS             403..897
                     /locus_tag="ACHAUG_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481412.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(1059..2231)
                     /locus_tag="ACHAUG_002342"
     CDS             complement(1059..2231)
                     /locus_tag="ACHAUG_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8481413.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2437..3252)
                     /locus_tag="ACHAUG_002343"
     CDS             complement(2437..3252)
                     /locus_tag="ACHAUG_002343"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8481414.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3402..3848)
                     /locus_tag="ACHAUG_002344"
     CDS             complement(3402..3848)
                     /locus_tag="ACHAUG_002344"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8481415.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3845..4360)
                     /locus_tag="ACHAUG_002345"
     CDS             complement(3845..4360)
                     /locus_tag="ACHAUG_002345"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8481416.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(4433..5347)
                     /locus_tag="ACHAUG_002346"
                     /pseudo
     CDS             complement(4433..5347)
                     /locus_tag="ACHAUG_002346"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(5344..7671)
                     /locus_tag="ACHAUG_002347"
     CDS             complement(5344..7671)
                     /locus_tag="ACHAUG_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM8481417.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            8280..8426
                     /locus_tag="ACHAUG_002348"
                     /pseudo
     CDS             8280..8426
                     /locus_tag="ACHAUG_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(8514..8807)
                     /locus_tag="ACHAUG_002349"
     CDS             complement(8514..8807)
                     /locus_tag="ACHAUG_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8481418.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            8844..9029
                     /locus_tag="ACHAUG_002350"
     CDS             8844..9029
                     /locus_tag="ACHAUG_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481419.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(9210..10388)
                     /locus_tag="ACHAUG_002351"
     CDS             complement(9210..10388)
                     /locus_tag="ACHAUG_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8481420.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(10492..10806)
                     /locus_tag="ACHAUG_002352"
     CDS             complement(10492..10806)
                     /locus_tag="ACHAUG_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481421.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            10913..11128
                     /locus_tag="ACHAUG_002353"
     CDS             10913..11128
                     /locus_tag="ACHAUG_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481422.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(11428..11670)
                     /locus_tag="ACHAUG_002354"
     CDS             complement(11428..11670)
                     /locus_tag="ACHAUG_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481423.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(11657..12085)
                     /locus_tag="ACHAUG_002355"
     CDS             complement(11657..12085)
                     /locus_tag="ACHAUG_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8481424.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         3535 a         2372 c         2111 g         4141 t
ORIGIN      
        1 ataacgtaca tattttatta ttgcatactt aataacagaa ctatagcaaa aaatcccgtg
       61 tatcaacacg ggattagatt aagtttcctc aatcactatt cataacacaa ttattataac
      121 aagtaagtaa acaattgaaa aggacattta ttgaaaacgg ttgtttttta cttaaggagg
      181 aaatttagcg aaaaaattag ttgcattgtt tttggtcgca ggaagtttgt tgagtgggtg
      241 tggtgctact acgtctacta ctcaaagcac aagttcagat caggcttcca gcagcagtac
      301 cacgatctcc gaaagtacgg cagattcatc gaaagaacaa gaacaaattg aaaagaaaaa
      361 agaagagaaa caaaaagcgg aaaaagctgc actagaaaaa aagtgacaga agcggaatct
      421 gctttgtcac aagctgaagc aaatcctaca aaagaaaact atgcaactgc ttcagcagct
      481 atccaagcgc tacctgaaaa caaacaagag ttgacaagcc gtcttgctgc aattgattcc
      541 acaatcaaag caaaagaagc agaagaagaa cggaaaaagc aagaagaaat tgcacgacaa
      601 caagcagaac aagcgcaaca agaagcaatc gcacagcaac aagcagaaca ggcgaggcaa
      661 gaagcaatcg cacagcaaca agcagaacag gcgcgacagc aatcagcagc tgaacaggcc
      721 tctcagcagg cacaagccgc agctcctgag caaaataatg aacaaactgt ctatgtcaca
      781 ccgaccggtt caaaatacca tacacataaa tgtggaaatg gtacgtatac ccctgccact
      841 ttaagcgaag ctcaagctcg tggtttaacc ccttgctcca aatgttatgg aaactaattc
      901 aaaagttata ctataataat aaaattcttc tgaaacataa aaaaatagaa gatggcgttc
      961 cagatagtaa accaaaaata acagtttaca cgtccatttc gtcagcttct ctcttttttc
     1021 atttagtctg ttccctctgc ttttttggac atcttcattc attctttctc aatcaaatag
     1081 tacacctctt tttcttttcg cagtttatat cttccttttt tcaatttcat tgaaaactgt
     1141 ttcgaatgtt tatgttttac atggatttta agcaggagct cctcatgttg caagcgatag
     1201 ttcctgcctt ttaaatcaga aaatctcact agtccgtatt catcagaagc tttcacaaat
     1261 ggaacttttt ctctagtaat atagtgtttg ccgttgagcg tttttacaga aaagaccatt
     1321 ccttcgattg gtcgtccgtt tgtgttcttt acataaaaag ataaaggata gtttcgctta
     1381 gcgatggcca aagaatctaa ataagcgatg agtagccaaa ctaagccgat tgatccaagt
     1441 agtataccga taaaccaaat aaaaaatttc cattttttcc attgatcaat tccttggatc
     1501 gctttttttg cctcttttgc ttgataagga atacgatgcc ccgtcacaag cagtcgatgt
     1561 gtattgacca tataaggagt acaggtaagc aaagtcacga gatctctgcc tttttggata
     1621 cccaaagaat ctacttcatc aggtaagacc acttggattt tttctacttg atacgccaat
     1681 gtctttccat tgatctggat gaaaaactgg tctccttttt tcaacttagg caaatctgta
     1741 aataattttg cttctgggag tccacgatgc ccagaaagaa cggcatgggt gtttttcccg
     1801 ccagtcggat aagaactttc ttccaataat gacgttcctt tttgcaaaaa tatttccgtt
     1861 gtctgatcga atataggtaa gctgacattg atttttggga tttcgatgac tccaagcata
     1921 tgttccataa aaaaggcatt actaggtaaa tcttttaatg cttttgcatc aactgcttgg
     1981 ttgaaactgc ctatccctgg tgatacgttt tgctctgcca actgttggtt tttctttgtc
     2041 attttttcct gctgtttttt tatttcctct gaattcttat tcgatgcttg cttttgataa
     2101 tggatgatca tttgctgggc aagaaaatca ttcagtgaat cttctacaaa aggataagaa
     2161 aagatcccga tcccaacgac taaaatgatt gccatgaaga tgtccagtat tcttattctg
     2221 cttttattca tcatgtccac ctattttcta atatgtatat gaagaggttg tgaaatcagc
     2281 gccttgacct gagtattagc gaagcaaacg gcaaaatagt tcctcctatt ttgccgtttg
     2341 ttgagcgaat accaagagac cgaccacttg tcacaacctt tctccccatt tgaatcacta
     2401 tcgtttattt atcgtatgat ttttgtttcc aatagtttaa gcttctgttt cttgatggcg
     2461 ttttttgaaa tagatcactg ctcctccaac taaagcagta cctaccaaga tgaatacgat
     2521 gatccccata ccaccagttg aaggcaagat tcctttaggt tggttatcga tcgttgtttc
     2581 atcagaagtt gttggatcat catctactgt aaattcagtg tacgcagctt cattcttagg
     2641 taatgcgtag ccttctggtg cttttgtttc ttgaaggcag tatgcccctt gtttcagccc
     2701 ttcgattgaa aatttccctt ctgaatcaga aatcaatttc acttcttccc agtcagaacc
     2761 tgtgacttca tctgaccaag catatgggta ttctcctgag gcaaggtttg ctattttttt
     2821 cattaattta acagttggat cttttgctct tagaatagaa aattctgcat cggctaattt
     2881 ttcttgcgtg ttcacatctt gtttgatgaa gtggaaatca ccgtagtttt ggacattttc
     2941 tacagtaatc gtgttatccg tagttgtatt ttcccgattg actacaccgt ttggattccc
     3001 aagattggct tcatctgcgc catggatgat accggttgtt tgttgcaatt ttccgttttt
     3061 tactataaaa gtagtttctt tttgtggatt ggttaattga taaccatctg gtgcttttgt
     3121 ttcaactaaa gtataattgc cgtctattaa tccattgaca ctgatggatc cttcagtatc
     3181 agttgtgaat gtcaacgctg actcagcagg atcatagaga gcgacaccag tgttttcatc
     3241 aaaaccggtc acgaattgat tctctgaatt tttgatctgg aattgcgccc ctgctaactt
     3301 atctacccct gaaaagttcc cagctttctg gattttgata ttgctttctt tgatgacatt
     3361 tttagcatat aggtgaatcg tgtctaaagg tttgtcaggg tctagggtaa gcttaacacg
     3421 atatttggcg acgtttcgac accgggcttt ggtgtttcta caaagacgta tacagcatct
     3481 ttcccatcag atttacctgg aagagtcagg ttagctaatc cttcaccaga agtcgttaca
     3541 cttgcgaagg gtgtagcgtc tgctggaact gctgtgtctt tacttggata gtacgtagca
     3601 gtgatgtcat atacgttgaa ttcaacgccg ttcaacggtt cgctattcgg aaatgtcaat
     3661 tccgttccgt tgtttatttg attttctggt gctgaagtga atttcaattt atgcaaatta
     3721 accgtaacat tttctggtgt aggtgatacg gctgcagaaa cctctgcagt tcctaggccg
     3781 agaacgaaag gaagcagcat gatagcagtt cctaaaaacg tccatatttt tgttttgatg
     3841 tttttcattt tatttccctc ctattgtttc ctttttgttg ttttttataa aaccaagctg
     3901 tccctgttga aaggactagc aataacccta aaattgagaa acctccaagc atctcttcgc
     3961 ttgtttttgg aaagcttcct ccattttttt catttgatgg accatttgtc tgcatctgaa
     4021 ctggcgtttt tccattattc gtcggatttg cttttggttc gttgatgatt tccaatggta
     4081 taggatgaag cggatcataa ctgccttttt caacttcaaa gagaatggtt gattgactca
     4141 atatatatcc tttaggcggt ttaatctcca ctagttcgta acttccaaaa gacaatccat
     4201 ggacagaaaa tgttccatct ttactggaag taaaaatcgt tgctcttgaa tcatctttgg
     4261 ctacccaatg atttatcccg ttttcttgcg ctaagtattg gtcatttttt ttgactagaa
     4321 attgagcatc tgctagtttt tgtttcgttt gccgatccac tttgacaaat tgtttgcctc
     4381 ctgtttggat ggcaagatgt gtttctactg tttgattgtc agtcgtcaaa cgtacatcgt
     4441 tatcaaatgt cgttttttca ctgttttctt tcaatgtcat ttggtaacgg atcgttaatt
     4501 tttttccagc aaacgaacta agatcttttc cagaatctga taagacaaaa ccatgtttta
     4561 ttttttgtgt gtgaaatgta tgaatcttgt ctccaccaat caaaaaatcg atcgtttctg
     4621 gttctagcca taactgagga tcagctgtat ctttgatcgt ataggttcct atctcgttga
     4681 tgttctcagg aattgttacg gtgatgagat aagggacttt atccccatct gcaaaatctg
     4741 tctgcttatt ctctactgtt tttgttaatg tgagtgcagc aggtattttt tgttcgctct
     4801 ttggatacaa gtggatggtc gtcaatttat gattagatga atctgtcaca ggaagcacaa
     4861 cgacaaatgg tgctgatttt tcttttactt aatctgaagt ctttatttcc gtaaatcggt
     4921 aaacagcgtc acgaccttgc tcatctttgt ccgatgcact aaaagaagca atgccctctt
     4981 cctcgtctac tgtttttgtc acattttctg ccagtatttt ttcactatca cttttttgtg
     5041 ctagttttct ttgtgcgtct tccacactac ttccttctga ccgcagcttg taaaattcat
     5101 ccgagacatc atatatagcg aaagtaacgc cgttcaaccc ttttactcct tgaaagttat
     5161 ccgggatatt tattccgtcg ttttgactga tacttggcat cttatcgctt tcgaacattc
     5221 gattatgcaa agtaaatgta ataggtgatt ctctagctga cacagtcgga gaacctataa
     5281 aaaagaccag taggataaaa aagagaacag caaatatatc cacacgtaca ttttttgttt
     5341 ttttcatcgc tgttcacctg cttttttctt acgatggata aaataaaaga agccagaaaa
     5401 aataaagatc agtcctaaaa agacaaaggg cagtagacct atcccacctg tcgaaggaag
     5461 caagagagtc ggattgatcg tcaacttagc ggtatctgtt gcgataatcg tataattatt
     5521 gtttacactt ccttcgccta aactaatcgt tactatttgt gatccaatat ctgtccggct
     5581 gttaactgtt agggtataat ccaatgattc gcctaagaca acaccggtaa cgtggagatt
     5641 tccaaccgaa atcgagtgac tttgggcatc ataattaaag gcagcgtcat cgacagtaat
     5701 cactgcgtta gctttataga tggtataagt tcctgatgtg ttggctatat cagtaaatga
     5761 caacttggga tttgctgctt tgactttgtc tattccgcta ttcgttagtt taattgtata
     5821 gctgccagca ttaaccatat tttttgctga ttgtgcatct gctgtgtaat ccatactgct
     5881 aagtgccact tgtactggag taagcgaact agtgacaccc tctgctagct tgacatggat
     5941 tgacgggaca cctgcttttc catcatatgt cttactgcca ctgagctcta ccgcaccgct
     6001 gatttgctta atcgtataga catcagatgt ctgcacgata gtaaataaat agtttgttct
     6061 ggcagctagt ttagctagtc cgctttttgt caattgccaa gacgtatagg ttccaatttc
     6121 agtaggcgca gttacttcaa ctcccttttc attcatcgct ttgaaatcag ttggattcca
     6181 tgtgatttga tcaatactga gatcattgtt taaaagggat gttgattctg ttttatcatt
     6241 aaaggcatat ttaacgatcg gtacaacaga aagaccatca tagtacttac tgccatttgc
     6301 attgattgat actgttgcat ttgtcttata atataggatg acttcagaaa tctgttttcc
     6361 atttgcgtcc tttacgatta catttccatt tgtatctaaa ctagctaaaa cactcattgc
     6421 tgattggttc tttgtttcta tcgctgaaac agctgtaaag gtatatccgt tgatagtagg
     6481 cgtattagtc gtcaggtctt gtgcagcact tccgtaagta agattcatta ttttagaagg
     6541 agcaatcatc tgtccaaaat agtcttgata cttaatagtg attggtgttt gtttgcctgt
     6601 ataggtcacc gtatcgacgt tactttctag ggctgtgaaa gaaccgtttg tttgtgcaaa
     6661 agaatagccc gttactgctg ttggagtgat aaccactttg tcacccacat ggattccaga
     6721 taatgtagtc ccgtacgttc cgatttttgc tgctccacca ttgactgtat aagaaaaagt
     6781 tcctgtccga ttcgcattga cctgaaccgt tgctgcttga taggttggaa aatttgaaac
     6841 atttccctta ccatcaacga tctgcccatt tttgatcgag taccccgtga atggaatatc
     6901 atttgctaac tgtataccga ttgaaaaagt actcatacct cctccggcag gaagccctcc
     6961 ccatgttgag ggtgtaaaag aataactttt atccgcattg ggtgtaactg aatcgatcgt
     7021 atttccagcg gtagccaccc ccatatagat actttttggg aatgtgatag tagtaccatt
     7081 tactgtggag ccaataaaca gattatggat atctggacgg ttatcaataa aagcgattgc
     7141 tttggttacg ccttgtaacg cagttttatc cgcactcatc gatccgttaa ccgcaacaat
     7201 gactccatct ttggcatata tcactgtata tccatctggt atggtatgtc cgttgaaatt
     7261 cgcagtggcc gcaacagtac taaatgtctg gcttctacct gtttctgaat aagctttctc
     7321 ccctgctgct tttgctgctg tgatttcttt ttctgtcgga tcaatcagtt gagtcgagga
     7381 tgtggttgag gttgtctcgt tgttgctaga ctgattggtg ctttctatgg actgtatttc
     7441 agaatagggt ggactatttg tctcctctgt cgtttggtta gcaaaatttg acgtttctgt
     7501 ataaacgttt acataaatac ttgataagct ccctaagaaa gtaaaaatca agatgatcga
     7561 aatcataatg ggatgtaagt attttctttt agatacagct ttgagtctag aaacaaaatg
     7621 tttttgacat atacgttgga ttttattttg agttttctta atcattttca tccctcctct
     7681 caataaaaaa tcatcaaata atcctttaca cgttattcta aatttaattt ttattatttt
     7741 attgatgtat agaaagtttg gggctttttt tctcttttta ttctaaggtt attttagtct
     7801 tgtcctttaa tacaaagatt ttatttttaa ataaaaaata ctcttttttt acgattattg
     7861 aaaaaaaaag ataaaacttg attaattgaa gtattgctta aatttggaag ctatgaggga
     7921 agaataaaag agttaggacg ggattatcta ctatttttct ttgttttaaa tatttgttat
     7981 ctttgaacat aaaagagaaa tgtgtcgact gcagtgttgt gtccctcatc acgaataaac
     8041 ggtgttcaac tagctgatct tgtattcact taaaaaatgg taaagtacga gttgctattc
     8101 cccattttca attttctaac gctcaggaca aaatactgtt ttcacaacct cattggaata
     8161 aaagcatata tattgtttca agaacgaaat cttgttatct ttttaaaggg catctgtcca
     8221 aaatctaatt ttaggaggta cgttttggta tttattcgta caaataaaag agaattatct
     8281 ttattcatgt aacgcatcca attgattttt tcatgtacgc tcctggaacg attttactga
     8341 cgattgctta tttaactgcg aatcgctcat tagcttttat catagctatc catattttga
     8401 ataacgttct tggctttgta ttatagatac agatgacaaa aaaagttcaa gtatgcggtg
     8461 tagaaccaat atcgcatact tgaacttttt tcaatgagta taaattaaaa cttctaattt
     8521 tcaattccca tcatctgata tgcccgaccg tctgaacttc ttttgaaaaa gccaaatgtg
     8581 attagataac gtctttgttc gacataatcg ccactagaaa atacgttcag cagtatcgaa
     8641 ttaacctctg tttctgtata agtcactcca tggtcaaagc gtttcaagac ttctttcaat
     8701 aggatgtatt tcttttttgt tgaacttggt acttgtttta cttttccatc attcatgaat
     8761 gtgtctaaaa ctttcatctt ttcttgctcc gtataagtat aaaccatctt ctttctcctt
     8821 ttgattctaa gaatactcca tttatgttag aatattctgg attttttatt aaagcggtta
     8881 ctcttaagaa agatattatc atagtgctct tttttttact actacttata ggtagattta
     8941 ttctaatcca ttattctccc ctaccaataa ttaaagaaaa aactggatca gggtgttcaa
     9001 attacatgct aagaaaaaag aacccttgaa taccaagggt tctttctcag tctgataaca
     9061 atgattaacg acggatttct ttgatacttg tgaaaagtcg ttaaagtatt gatataaaag
     9121 gatttttatt acctctgact acatattgac taatttgtta aaaaatcgtt tttcaagaat
     9181 cctgtaatac cagtgctttc accgtttttt taactctcta aagaactaaa agcaatcaga
     9241 ggttttacct tttcatttga cgttaagtgg cgataaattt tcgaagttgt ttggtcagta
     9301 tggcctaagc gatctttcaa gacttcccag ctacagtgtt ccgctactaa gcttgccatt
     9361 gtatgacgag ctaaatgagt atgaaatggc ttattaattc ctgagcgatt cacgcaccgt
     9421 ttgacgtgat catttacata ctctcgacga taaggaacac cacgtttact agaatcaaga
     9481 tacacaaaca aatatgtttc ttctataata aacctgtaat gttgcctcat ctcacccaaa
     9541 gccttaatca actgttgtag cttttccata actagaggag ttactagaac tcgtctagca
     9601 gaactaactg tcttagtagt ttcaattcga aaatccttac tcttaccttt aactgccgtt
     9661 tgcttataga acagaatttc atttcttttg aaatctatat ctccaggctg tagagcaagc
     9721 gcctcgctga ctcggcagcc agtgtaaaat agaacatgga aaagtacaga catcggtaat
     9781 tcctcatgaa caatgccata attcaaaaaa gttctcaatt catcgacagt aagaaacttg
     9841 tcctcaatat tatttatggc ttcttttaaa tcagttaccg ttaactgata ttttggcatt
     9901 ctcacagact tcattggatt ttcacttatc aaattctttc tttgacaata tttaaaaatc
     9961 atatttagaa cttgaataag tgattgtaaa ctatttttgg catagccaac atttgttcca
    10021 ttaacgccac attcctttaa ggtaaaaagc aattcttgaa ggcactctgg tgtcaaatca
    10081 gacaactttt tatcaccaat ccatcgaaca ataattttta attgttcctc tcgcttatca
    10141 taggttattt ccttttgttc atttaacgaa cggtaatatt ggaaccattg ctcacctaca
    10201 acactaaact tgtcattgct tgcaacaatt tttttcttaa gcctttgttg tttctccatt
    10261 tccagataag cgagttgcgc ctcacgtttt gaattaaagc cacgtcttgt tgttcgatat
    10321 gcttgaaagt accatgtgat cctaccagta gaactgattt gcttcttgaa tcgatctgtt
    10381 ttttccattt attgattcct cctattttga aggaacccaa caagcagaaa ccctttggaa
    10441 atctttctat ctatgtcatc attgtaccgc atcttagaaa gaaaatgtaa cttataaaga
    10501 tcgaagtagt cgcaattttt ctcgatagtc attcgaatca ctctttctca tccattctaa
    10561 aacatcatca cgaaaatagt gtcctcgaat cccgtaccaa cagggaggaa actcaccaag
    10621 ttttactaaa cggttcagtg tactatcact tatctgaaat agtcgcttta aatcagtatt
    10681 tgttaatacc agaggtaaat gactgactgc aagcaatttt tccgtgtaat cttccagttt
    10741 tttttgtaaa attgaataga actctatttc cagagaatta acatttgttt taacttccaa
    10801 ctccatcacc cttctctctc aaaatcaaaa cgaccaccac ggtacccctt aaaattgtaa
    10861 aaaaacactc cagtactcca tcaaaacgga ctagaaaaga ggaattttta atatgaaaca
    10921 aggaattgca gacataaaaa taattaaaga aattcttgaa aaatctacgg ctaatgcaat
    10981 agcattcgga acaggaatca acttgagtac agtaaaaaaa cttaaaagtg gagagagagc
    11041 tgaagaaaaa ttaaatcttg ccgatgctat taaaatcaca gaatttggca tgaaaaatat
    11101 gccaaccaaa atcgaaatat ggaaatagta gtttttctga tctatcagct gttttgccaa
    11161 agcacatagg aatctcacct ccccccagta atggcgggcc gatttggtta cggaagtatc
    11221 attatcgtag tcgcgattct catattaaaa tttgtcgctt aaaagctaac cttgtcatgg
    11281 cgtaactaga gaatcaaaat ctacatgtac tgatagattt gctattcatt tgtcaaagag
    11341 cgctaggcta ttttcagcct atggaaatcc tttattttga cccgataaaa aacttccata
    11401 gaatgaaaag gaaagggaaa aaatacttta acgagttgta tcaaattcaa gtatcttctc
    11461 aatcaatttt gtttccagtt gtctacgcat atattcatct acatacataa ccattccttt
    11521 ttcttcttca taaactggtt tcatggctaa ccgtgaaata taattttgat attttgacag
    11581 aacgatggac atggcaattg aatcaccaga agctgcggct ttgattgtcg ttagtggtat
    11641 gaatggtata tatcgtttat tttgcttcat atttgacacc caaatacttt cctagctctt
    11701 ccaaagccat ttgtcgttgg taccaaacac tgccgagact tttcccaata atatcgccaa
    11761 tttcacgatc attaagaccg atgaagaagg acataaaaat gatttcttgt ctttgactag
    11821 gtaatgctgc aatcgcattt gccaactcct cattggaaat aaagattttc ataccaagga
    11881 caataaaagt accactacta ttcaaatttg gttccaaata atagaactct gtacctaacc
    11941 ctttttgttc caatatttcc attgatattt gccgttggcg aaatctagcg tacttctttt
    12001 gaatatttaa cgcctcattg cagactactt tcttacaaaa actgtcaaac gtatgttgaa
    12061 tgtgggtttc ctctgagtcg cccataatat tctccccctc aaaaaattca ttcaactcgt
    12121 tttacttcta tccctttcca ttattactac aatctagga
//
LOCUS       DAYYBH010000080        11910 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000080 DAYYBH010000000
VERSION     DAYYBH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.083"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACHAUG_002356"
     CDS             132..344
                     /locus_tag="ACHAUG_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481425.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..742
                     /locus_tag="ACHAUG_002357"
     CDS             506..742
                     /locus_tag="ACHAUG_002357"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM8481426.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITGVSIKS"
     gene            800..1926
                     /locus_tag="ACHAUG_002358"
     CDS             join(800..1028,1028..1926)
                     /locus_tag="ACHAUG_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8481427.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            1971..3158
                     /locus_tag="ACHAUG_002359"
                     /pseudo
     CDS             1971..3158
                     /locus_tag="ACHAUG_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            3278..3478
                     /locus_tag="ACHAUG_002360"
     CDS             3278..3478
                     /locus_tag="ACHAUG_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8481428.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            3554..3751
                     /locus_tag="ACHAUG_002361"
     CDS             3554..3751
                     /locus_tag="ACHAUG_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481429.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(4048..5595)
                     /locus_tag="ACHAUG_002362"
     CDS             complement(4048..5595)
                     /locus_tag="ACHAUG_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFM8481430.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(5679..6482)
                     /locus_tag="ACHAUG_002363"
     CDS             complement(5679..6482)
                     /locus_tag="ACHAUG_002363"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8481431.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(6485..7267)
                     /gene="phnE"
                     /locus_tag="ACHAUG_002364"
     CDS             complement(6485..7267)
                     /gene="phnE"
                     /locus_tag="ACHAUG_002364"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8481432.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(7264..8043)
                     /gene="phnE"
                     /locus_tag="ACHAUG_002365"
     CDS             complement(7264..8043)
                     /gene="phnE"
                     /locus_tag="ACHAUG_002365"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8481433.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(8015..8806)
                     /gene="phnC"
                     /locus_tag="ACHAUG_002366"
     CDS             complement(8015..8806)
                     /gene="phnC"
                     /locus_tag="ACHAUG_002366"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFM8481434.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(8867..9796)
                     /locus_tag="ACHAUG_002367"
     CDS             complement(8867..9796)
                     /locus_tag="ACHAUG_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8481435.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            9991..10674
                     /locus_tag="ACHAUG_002368"
     CDS             9991..10674
                     /locus_tag="ACHAUG_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFM8481436.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            10835..11878
                     /locus_tag="ACHAUG_002369"
     CDS             10835..11878
                     /locus_tag="ACHAUG_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8481437.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         3770 a         2342 c         2318 g         3480 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 tggtgttagt atcaaatctt agacagcttt tcgtagagac aagaataaat taaacttatc
      781 tacgagagga tgaattttta tgacccgata tgaagaaaat tttaaacaaa tgattgttga
      841 actgaatcaa actggacgtt ctgttcgagg gttagcgaaa gaatatggct tatctgaagc
      901 aacgatttac aaatggaaga atttatattt acctgatcag tccacaggac tgactggaaa
      961 agaagtagct gaactgagaa aagaaaatgc tcgtttaaat gaggaacttg aaatcttaaa
     1021 aaaagccgca gccatattct ctcggaaaac ctaaattcgc ttgttcaatt tattgaaaaa
     1081 tggtgtaagg attacactgt ttctttgtta tgtcggttat tagaaatccc tagaagtgtc
     1141 tattattttt ataagaataa accgttgaca gctacagaaa tcagaaataa taagttgaaa
     1201 aagaagattt caacaatttt ttttacgaat aaacaacgct atggtgccac aaagatccat
     1261 caagttttat taaaagaagg gatttcagta tctcttaaac atgtccaaaa gctaatgaaa
     1321 caattaaatt taaggtcgat tgtagttaaa aaatatagac ctcaaaggtc taataaaccg
     1381 atcatttcaa aagagaatct cttaaatcag gacttttcta ctgaaacgat ctgtgagaaa
     1441 tgggcagctg acattacgta cattcctact aagaaaaatg gttggtgtta cttatcttca
     1501 atcatggatt tacacacgaa aaaaattatt agttatacat tttcaaaacg aatgactgtg
     1561 gattgtgtca ttcaaacatt gaataaagca aaaatacatt atcacattcc agaaggaatg
     1621 attctacaca ctgacttggg cagccaatac acagcaagag aagtggaaca atggcttaaa
     1681 accaacaaaa taaggcattc ttatagtaga aaaggaacac cttatgataa tgctggaatc
     1741 gaatctttcc atgcctcatt gaaaaaagaa gaagtctaca cgactagcta ctcagatttt
     1801 gaagaagcaa atcgagcgct atttagctac attgaaggat tttataaccg aaatcgaatc
     1861 catagctcga ttcactatct aaccccacag gaatttgaag aattagcaaa agaaaaaatg
     1921 gcttaaccgt ctctactaaa atgtgtccaa gatattgact caaatccatg atttacaaag
     1981 gaactcttct gtacaagcca gaagagtgtt cccattgtct ttgtgtcgtc ccctctagga
     2041 tcattcgttg ggggacgaca accgttcggt tgttactgaa tgatgcgtct gagtatcgca
     2101 cttatctgga gttgaagaaa caacgattta aatgtaagtc ttgccaaaga acattcgtag
     2161 cggatacctc tgttgctgaa aaacactgtt tcattagtca aaaagttcgt tggtctgtca
     2221 ttgctcgctt aaaagagaat acttccatga cggaaatcgc tcgtcagaaa aatatttcca
     2281 cttcttcggt ctatcgtgtc atgaagcgat tctaccgccc attgaatcct ttcaaacagg
     2341 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
     2401 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
     2461 gcttgcctta tttagaatgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
     2521 aatggattgt tattgatatg tatgcgcctt atgtttcatt ggtgaaaaag cttttcccga
     2581 atgctcagtt gatcatcgac cgtttccaca tcgtccaaca tattggcaga accttcttga
     2641 atcatcgagt gaaagaaacc acggcacggc ttaaaggaac ttcccactct caacgagggt
     2701 tagggaaaaa attgaaacgc tattggaaac tcctacaaaa agaagaagca aagttaaact
     2761 acgaaaaaag ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgtcg
     2821 atcgactgtt atctgcttgt cccaacctgc gccaaggcta tcagttatac caagacattc
     2881 tttatgcggt aaagaaaaga gatcagtcat tatttgagga ctgtttaaac agagaagtca
     2941 gcggtcttcc tgaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
     3001 tccaaaatgc gcttcactac agctattcaa atggaccatt ggaatgttta aacaaccaca
     3061 tcaaagtatt aaaacgcaat gcgtacggct ttcgcagctt ttacaatttc aaactacgga
     3121 tcatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa gagagtttct
     3181 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
     3241 gcctgtattt attactgtcg aggacaatgg catcggcatg attgaagagg aacaaaaaaa
     3301 catttgggat cgctattata aagccgatcc gtcaagtaaa aatacaaaga taggcgagtc
     3361 aggtcttgga ttatcaatcg tgaaggaact agtgcgcctg caccacggtt caattgaagt
     3421 gaccagccga aaaaatgaag gaacgacctt cctgatacaa tttccaaaag aaaaataaaa
     3481 ttaccagtaa aaaaccgtgg agagatagct gctattgatt tttgctatgt cttttctttg
     3541 agaggtatcc ggtatggtgg agtgtctgaa aaataaaaaa atggaggcca attttgtgaa
     3601 agctgttatt tctggaagta ttgttttcca agaagaatat cgagatatgg aaaaagattt
     3661 cagtaaaaaa ggcattgaat taattgatta tcctcgctca tgtccgatcc tagacaaaga
     3721 gtatccagaa atcttgacga ctttttttta aatatcgcag cagcggattt attttttgta
     3781 tttaatcaag atcggaaagg ggtattaggc tatattggtg ccgcaacttt ttcagaaatg
     3841 aagtattgtc tgatgcaaaa tcttattcat ggaaaaagga tcaaaatagt cttatttaag
     3901 gaaccatcag aggaaaattt tttgttttga tgaggtagat cgctggctaa agaatggctg
     3961 gatggaaatc agacagccta acaatttggc ataacaaagg tgtgggcaaa aaactgctca
     4021 cacctttttg aattttttat ttatatacta caaaatcact tgaaaattat gattcacttt
     4081 tgcttcgatg cgtgggtgaa tattcaaata gtcggcaatt aattcggtca tgtcgatttg
     4141 gatttctcgg acaattttct ccgcaccaaa catcgcataa tttcctccgc cgattgcccg
     4201 atattgattt gtcaccactt ccagcatttg ttgcggaaga acggggtgac ctttaaaacg
     4261 aagttcggta atccgttgtc caacaggctg tttcatatca atagtgtact gaatcccttc
     4321 atacatgtca taattgtagt actggggctt aggctgaata aaggctggat tgaatacgat
     4381 ttggttattt tctacagcta aataatttgc ggtctgctcc aacgccgctc tcaaatctgc
     4441 accatttatt tgaatcactg ccagagtatt cggataaata tagttggtga tgacgtcccg
     4501 catcgtgatc gtctcaccaa agccttttcc ttcattattg aataacgcag ttccagaaat
     4561 ctctgagccg ctggcaaata attgtacacg attaataaac tctacatagg gatgttcctc
     4621 aagacgagcc tgcatcggat cattaatacg catatttccg ttcactttac ctaagggttg
     4681 atccaaccat ttctctagtt tttcttctac cggtacgatc gctgcaagaa tttctggatc
     4741 aggctctgat ttttcaactg agtgtaaagc aacctggtga tctgtaacaa ccacttgccc
     4801 ctcttttttc tcgacggtaa gacgaatttc accgatgaac tcgccacgat agcctggttg
     4861 aacaacggga actccatgaa ccgtttcagc aattttccgg tgctgatgac cggtgaaaag
     4921 ggcgtcaatg ccttcaacct gctgcaatag ctcgtagcct tcattctctc cagttagcgc
     4981 ttctgtgtcc tcaccggtta acaagtcttt ttcaaatccg ccgtggtaac taacgattac
     5041 caagtctgct tgtttcctta atagaggaac atattctttt gctgtttcga cgatactctt
     5101 aaacgtaatt cctgctaagg tttctggctt ttcccagtga ggaatgtatt gtgtcgttaa
     5161 tcccaagata gcaattttta cacccacctt ttcgataatc ttataggcgt ttccaaagta
     5221 tggttggtca tttttgttca ccatattggc tcctaatata ggcgcttgat aattcttaat
     5281 cgcttcctcg agataatcca caccatagtt aaactcatga tttcccaaca cttgaacatc
     5341 atagctcatt tggttaatca agtctgtaag ctctttggct gaaccatatt ctttagcaag
     5401 ataataactc aaaggagacc cttggatgaa atcaccattt tcgatcgtaa ttaccggtcc
     5461 atctgctttt gcccgtaatt ccttcagttt cgacgatact ttagccgttc ctaaggccag
     5521 atcggttctg ccggaatagt ctgttggtga aacatagcca tgcatatcac ttgtctctaa
     5581 aaacgtaatg ttcattctgc gtgctccttt agctgactca aaatagaaaa accgaagcgt
     5641 attcagctgt tcggtctctt tttttgattt ataatgcctt aatccttcat ttgaagtgtt
     5701 tttaaaatgt tgcctggaat aaagttttct gttgtctggc gaacaatcct tccttgatcg
     5761 agatgataaa ggttataccc atgacgttct tcaaagcgta cgaagccatc gccttcatct
     5821 tcaccaacaa aggacatact cgatacagta aaatagggga tcccattaat ttcatcagta
     5881 aatgtgtgat gggtatgacc gtttaggata cagcgaatat cgtaaggtgc gattagcttc
     5941 aagaaacgtt cggtaccagg ccattttggt atactgcttt ggtgagacag tagatgatga
     6001 tgggtgacca agaaaatcgg ttggtctttt tcttgctcta aggctttttt cagccattga
     6061 aattgctgat cgtccactat accatctgcg tatccatagc ttgaattatc aaaggaaatc
     6121 actgtaaact caggatattt gactatctgg ttgtacgggg cagaagaggc ggcttcatcg
     6181 caccagccta cccaaaaatg ttctttgata tcgtgattgc ctagagtaac aagaatcgtc
     6241 gtttctccta gcgcattttc cagccaagtc ttcaagtatc gataatcgtc tatttcgcca
     6301 tcttcggtta aatctccgct gataatcaca aggtccaagg gtgtttgctg caggagatga
     6361 tgaatgcatt gctccagagg gataagggga ttctgcatcg tagccagcat tcccttatag
     6421 ccttcttcac aagcctcata ctctctgcga aagtgtatat ctgatatatg caatatattc
     6481 attcttatct caatctcttt cttataattc cggttatttg atctaaaata aacacggtta
     6541 cgactacgcc aatcaaaata atactcactt tgctccaaga ccgagtttga atcgcgaata
     6601 tcaatggagc accgattccg ccagcgccaa ctaagcctaa ggtagccgca ctacgaaccg
     6661 ctatttcaaa atgatttaat gctaaagaac aataaatcgg catcaaatag gggatgcgaa
     6721 caaagaaaat cgtttgccaa aaatttgctc ctaccgcatg catttcttca acaagtcgct
     6781 catccatcgc ttccatttct tctgtaaaaa gtttgccaag cattccgatc tgatggacgc
     6841 caatcgccat aacaccagca aaggggccag gtccgacgac tttcacaaaa ataattgcaa
     6901 aaactagttc aggaaaagct cgtaaaacat tacagataag cttgccaatt tttgttaccc
     6961 atggatgtgc ttgccagagg ttaaccgcac tgatcaaggt aatgggaatc gctaataccg
     7021 tagcgatcat tgtccctaaa caggcgattc caatggttaa taatagcaaa ctaatcaaat
     7081 cctcgccgct gccatcatat agaaactgcc aatcaggctg acctaaccct ctcaaaacgt
     7141 ccattcccat ggaaggagag aaggtgctta atccagagta atccagagta gcggccgaca
     7201 aaataacgag aatcactaaa gcaactaatg ataaaagggt tcttctgttc atttcacgaa
     7261 gcatcataac agggacctcc tgacacgttc acttaattta tcaacgatcg cgacagttac
     7321 taggatcgtt agaataatga ttgaaactcg ttcataattc aaaagcgcaa gtgaggaatt
     7381 tagaataacg ccaataccac cagctccgac atatccaagg acagtagaag cacgaacatt
     7441 cacctcaaac gcgtaaaaag catagctcgc aaactgactc aaaacttgtg gagcgatcga
     7501 ccaaatagcg atttgcattt tatttgctcc aacagccgca gcggcttcaa tcgggccaaa
     7561 gtcaatggtt tcaattgctt gaaacattaa ttgtgacacc ataccaaaag taaagacagc
     7621 gatagtcaac acgccagtcg cttcaccaat tccaaacaca gcaacaaaaa gcgcggctaa
     7681 aagcagggta gggatggtcc gtactaaacc taaaagaaaa cgaaagatgg tagaaagaag
     7741 acggttttct gtcactacag tggtcgctaa aaaactaaca gggatcgcta aaattaagcc
     7801 aatgaccgtt ccaagcaaag acatttgaag tgttttcacc ataggctcga ctaatttagg
     7861 taaatagcta aagtctggat gaatgaagcg tgataaaaag acagtcattt gattggagtt
     7921 ttgaaaaacc tgagaaaaat cagctgacgt gatccaggca cttccttgaa aacaaacaat
     7981 taagaacaga agaataagca ggtgtttata ccatttaaag tgaacggttt ctttcaagct
     8041 catcgatttc gcctgccttt tcttcaaaga ttgcattgcc ataaatttgc tctaagcgtt
     8101 catcggttaa gtcgtctgga cgaccatcaa agacaacctg agcagctttt aacgcgagca
     8161 cacgcgtaga aaactcacga gccagaggga ctgaatgcag attgacaatg actgttttat
     8221 ccatcgtttg attgatattt tttaggtcca tcataacctt ttgagtcgtg attggatcta
     8281 atgatgcaac gggttcatcc gctaaaataa tgggggcttg ctgcacgagt gctcgcgcaa
     8341 tggatactcg ctgttgttgc ccaccactga gctcatcact gcgtgtctgc aatttatcag
     8401 acaaaccaac tttttccagt gcttcctttg tctgctggta gtcttccttc gtaaaaagac
     8461 ctaaaatact tttccaagtg gagtaatagc ccaaacgtcc agatagcaca tttttctgaa
     8521 ccgtactacg ctttaccaga ttgaagcttt gcgagattag accaatctgg cggcgaatct
     8581 gtcgcagctc ttttttattc gcttttgtaa tagaggttcc attaatgcga atatctcctt
     8641 tggtggtttc tgccaagcga ttgatactgc gcagcagagt gctctttcca gcaccactca
     8701 accctatgat ggaaacaaat tctccatctt caattgttaa gtcaatatcc ttcaagccaa
     8761 caacgccatt gggatagacc ttcgaaacat tgtcaaaaat aatcattatc cttctccatt
     8821 tttttaagtg taagcaaaaa aaagcttgct gacacttact gatgttttat tctgctgctt
     8881 ttttcgtgta gtctctgatc gtgtcatagg cagaatcagc agcttcttca tagccctgat
     8941 gcccccacaa agccattgct tctttgccat ctttatcatc cttcatattt aagaaggtct
     9001 ctttaatttt cgcttttaaa tcttttgaca tgtcaggctg tacagcgatt gcgtcattcg
     9061 gaatatcgcc ttttgtcagg tacaggactt ttaaatcctt aaataaatca ttatctggga
     9121 aattcgaagc aaaaacattt cgcgcgcctt caaaaacaaa cgccgcatcc atttgcttgt
     9181 tcaacactgc ggtaatctca ctcggaatat cattcacagt ggtcatcttt gtatctgcag
     9241 taggatcaat cccagcatct ttcaattcag cgacaggata gatgtagccg ctagcagagt
     9301 taggacttaa ggttgcgata ttttttcctt ttaaatcagt taacttattc aatccgctat
     9361 cggcacgaac caatacttct cctttaaagg tgttcgtaag tttgtccttt aatggcaagc
     9421 cagtttcttg atcataatcg ccgagctgag aagtcaggat tgcatctgca gctccagcat
     9481 cctttgcttg aacataggct gcaggcggca taattccgac atcgacttga ccagaagaca
     9541 tcgcttcgac gatcgttgaa taatcagtag ctaacgtcac ttctacctca cgatctaact
     9601 ttttcgataa atacttagca aatggttttg cttttgcctc catactacca tcattgtttg
     9661 ttggaacgaa ttgaacaact aatttttctt tgctctcttt ttctgagctg ctacttgtat
     9721 tcccagaacc gcaggccgcc aaagtggtca ataacatgac tccgatgaac ccttttgcta
     9781 actgtttgaa ctgcattttt ctcctcctgt tggatataat ttctaatacg tctaaatcat
     9841 agcagggaat caaaatcaat gaagtgacgc acgtcctatt ttacacaact tttacataca
     9901 atttacaaac aattataaat aattattcgt attgagccga aaaacaatct aagctataat
     9961 aaagaaaaac ttcttagagg tgacgatttc atgaaaaaaa tccatgatac gaagcaatta
    10021 actgcttacg ttgagaagca caacctgcag gattatatgg attcagatat attggcaatg
    10081 gcttctttac ttttttttga aaaggaagag cacctcattc aaacgggaaa aacctcagat
    10141 tatttgtatt ttttagtgga tgggacggta atggtgtact cgtattcttc tgatacacaa
    10201 aatatttgca tcgattacgc aaagcccgct acaccactcg gcgaagcctc ttcattgtgg
    10261 gaattatcgc cgaaaagcag cgttaaagca gtgactaagt gcatctgtat cagcattccg
    10321 ctaaatcaat atcgaacaca gcttcaaaaa gatgttcgct ttttgcagaa tatttgccag
    10381 ttactaagtt atcgtttaaa ttccgggata aatttggcga actcactgac agaacctgtt
    10441 gaaactcgtt tagcaaagtt tattttgacc catcacgaaa acgaacgttt ctcatttcgg
    10501 ttaaccacct gcgcctcaat cttaaatgtc agttatcgac atcttttacg aacaatcacg
    10561 aattttcgtg aggagaatat tatcgaaaag aaaaagaatt attacttgat tcatgatatc
    10621 gaagctttag aggacttagc tgagaatata agtagtccag taaaagcaga ataacagcta
    10681 taaaaaaaca gcagtcagaa gtgattgctg gttttttgat agtggagaaa ttgggaaacg
    10741 gaaaatcttc gttgaagagt agtgttctat atattggtat gctatacttt taaaagttca
    10801 atgtgtcaag acacataaaa aggggagaaa aattttgaag aaaatacttg tttcattgtt
    10861 gtttttggtg tcattagtaa cgttagtcgg ctgtagtcag tctcaggggg agaagcaaag
    10921 tgaaaaagag aaaagtaaaa tcgctttggt tcttggattt ggcggggttg atgaccgctc
    10981 cttcaatcaa gctggctggg aaggattaga ggcttgggga aaagagaaaa agctgacagc
    11041 aaaaaaagat tttacgtata tagaaacgaa caatccagag gataacagta ccaatatcga
    11101 tcaggcaatc agaaacggct ttaccaccat tattagcttg agctacttgc aagcagagga
    11161 actggaaaat gctgctaaag ataatccaaa gttgaaattt gccttggtag atggtgagat
    11221 tactgggaaa aagaatatct catcagcaac ctttaaagat caagaaagtg cgtatttagc
    11281 aggactagct gccgcatata cgacaaaaag tgatcaagta ggctttattg gtggaatgga
    11341 agggcccgtg atccaacgtt tccaaagcgg atttgaagct ggcgttaagg caggggccac
    11401 agcactgaac aaggaaatta ctgttaacgt acaatatgct gcatcctatg attcgctaga
    11461 taaaggaaaa gctattgctg cttctatgta caaagaggga gtcgatattc tttatcaggc
    11521 ggcaggaagt actggagctg gggtgttcca agaagcaaaa gcccgcaatg aaaacgacta
    11581 tagcagaaaa gtatgggtga ttggtgtaga tcgcgatcaa gcggatgaag gggattataa
    11641 aatggagaac ggcgagacag ggaatttcac gttgacttct acaaagaaaa atgtcggtgc
    11701 agaagtcaaa gaatttgcag aaacttctca taacgataaa tttcctggcg gcaagcattt
    11761 gacctttggt ttgaaagaag aaggggtaga tctgacaaaa ggacaattat cagaggaagc
    11821 ctatcaggca gtttcagacg caaaagagaa aatcatcagt ggtgatttca gactgtagac
    11881 aaagtccatt ttatggatga tgtctacagt
//
LOCUS       DAYYBH010000081        11731 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000081 DAYYBH010000000
VERSION     DAYYBH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.084"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..1482)
                     /locus_tag="ACHAUG_002370"
     CDS             complement(364..1482)
                     /locus_tag="ACHAUG_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481438.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1715..1837
                     /locus_tag="ACHAUG_002371"
     CDS             1715..1837
                     /locus_tag="ACHAUG_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481439.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1953..3260
                     /locus_tag="ACHAUG_002372"
     CDS             1953..3260
                     /locus_tag="ACHAUG_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8481440.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="ACHAUG_002373"
     CDS             3379..4578
                     /locus_tag="ACHAUG_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8481441.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="ACHAUG_002374"
     CDS             complement(4605..5873)
                     /locus_tag="ACHAUG_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8481442.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5928..5994)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6036..6682)
                     /locus_tag="ACHAUG_002375"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="ACHAUG_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="ACHAUG_002376"
     CDS             complement(7090..7593)
                     /locus_tag="ACHAUG_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8481443.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="ACHAUG_002377"
     CDS             complement(7716..8480)
                     /locus_tag="ACHAUG_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8481444.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="ACHAUG_002378"
     CDS             8588..8863
                     /locus_tag="ACHAUG_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8481445.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="ACHAUG_002379"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="ACHAUG_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8481446.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10609)
                     /locus_tag="ACHAUG_002380"
                     /pseudo
     CDS             complement(10430..10609)
                     /locus_tag="ACHAUG_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11435..>11731
                     /locus_tag="ACHAUG_002381"
     CDS             11435..>11731
                     /locus_tag="ACHAUG_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481447.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         3560 a         2449 c         2076 g         3646 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaataacg
    11341 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    11401 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    11461 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    11521 ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca agcaaggtaa
    11581 gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg gtagaaaagt
    11641 cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc ttggttggac
    11701 gcctgacgtc attatcgggc gaaaagaaag g
//
LOCUS       DAYYBH010000082        11570 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000082 DAYYBH010000000
VERSION     DAYYBH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.085"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            373..579
                     /locus_tag="ACHAUG_002382"
     CDS             373..579
                     /locus_tag="ACHAUG_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8481448.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(781..1782)
                     /locus_tag="ACHAUG_002383"
     CDS             complement(781..1782)
                     /locus_tag="ACHAUG_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8481449.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1787..3700)
                     /locus_tag="ACHAUG_002384"
     CDS             complement(1787..3700)
                     /locus_tag="ACHAUG_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8481450.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3868..4374)
                     /locus_tag="ACHAUG_002385"
     CDS             complement(3868..4374)
                     /locus_tag="ACHAUG_002385"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8481451.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4534..4974
                     /locus_tag="ACHAUG_002386"
     CDS             4534..4974
                     /locus_tag="ACHAUG_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8481452.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5000..6115)
                     /locus_tag="ACHAUG_002387"
     CDS             complement(5000..6115)
                     /locus_tag="ACHAUG_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481453.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6160..6516)
                     /locus_tag="ACHAUG_002388"
     CDS             complement(6160..6516)
                     /locus_tag="ACHAUG_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481454.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6814..7788)
                     /gene="sgrA"
                     /locus_tag="ACHAUG_002389"
     CDS             complement(6814..7788)
                     /gene="sgrA"
                     /locus_tag="ACHAUG_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8481455.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7984..8823)
                     /locus_tag="ACHAUG_002390"
     CDS             complement(7984..8823)
                     /locus_tag="ACHAUG_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8481456.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9008..9895
                     /locus_tag="ACHAUG_002391"
     CDS             9008..9895
                     /locus_tag="ACHAUG_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8481457.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10489..11184)
                     /locus_tag="ACHAUG_002392"
     CDS             complement(10489..11184)
                     /locus_tag="ACHAUG_002392"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM8481458.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11168..11566)
                     /locus_tag="ACHAUG_002393"
     CDS             complement(11168..11566)
                     /locus_tag="ACHAUG_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8481459.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3445 a         2304 c         1735 g         4086 t
ORIGIN      
        1 tggtgaatta agtatttcct tgataacact ggcttttggc ctcattttag gttatcattt
       61 gtaattttat ttcacagttg atatgattgt tatgtaaaat aaaaaagttt aggatacatc
      121 gaatttttgg ggaaattctt ttgggggtat cctaaatctt ttctattata ttcccatatt
      181 ttctattatt ggtctagacg tatgatggta aactttgtac ctatctgatt gctgaatcat
      241 taattgctta tcattagcca tctttgtttc aagttcgcta gatgatcatt ttattcacat
      301 cctaacaaac ttgtcacatt tatagattct taatctattt atcatacaat tataaaaaag
      361 gaggtgctgc ttatgaaagc tatccatatt tctttgcttt ttttcgctag tgcattgatt
      421 ttatttgcta ttcatctttg ttcccagcct tatatcaata acaacggaat attagtggaa
      481 ccagcattct tttgcttacc actcggcttt ttgtcccttc ttgcctccgc aggcttcgga
      541 tttgttgctt tttttaaacg atcaaaacaa caaatctgac ttataaaatt tacgtaaaag
      601 agatcgtgac aataatcgtg tgatattccc aaatttcgaa taaaaggtgc cccttgccat
      661 ctctaactcc tcagtcacaa gttacaatat tcataaacca tgaaaagctt gtttattgaa
      721 attgctcctt gtgacttgga gctaagtgac agattacaac ctcttttgca tcataagact
      781 ttactccttg aaaaacatta ctaacttttg atatgtttct tgcgcttgtt tattgctgaa
      841 atcaaactga tattcatggt tcaaaacagc atttttctcc gcataatcta cttttgtgac
      901 tttcaccccc gctttctcaa atacagaagt tacttcttcc atttgtttcg taaacgtatt
      961 tgtatttcca tccgttaaga atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc
     1021 taaacttttg accttctctg attgttcaaa gtccttatta tcaaagtaag cccatccaca
     1081 tttttcataa agaaagctag agattttatc acttacttga gtaagtgcag aaggatccaa
     1141 cagaccagac aaagaaataa acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg
     1201 atcagctttt ttaatttctt cattttgaag tgctaacaaa tattctccac taatttgtgc
     1261 acctgccgaa tttccaccaa tcgaaatatt cgaccaatct gcttgatctt ttatctcttt
     1321 tttagctgct tgaatggctt cgttcaattg gataactggt gtaggatgtt ttgcttccgg
     1381 tgatcgttca taatctatat ttacgatgat ttgctgcgtg tctgaacata gatattccaa
     1441 ataatcacga caatcatttt tatcccctcc tacatatgcg cctccatgca tccagaaaag
     1501 aatcggtaat tttttctttt ccactttcgg tgtgtagata tccatattat tatttttgta
     1561 ctttgaagga taggatattt ctttttttat atcgatttgt tcgatttttt gctggaaatc
     1621 atctggtcgg gcatatactt gttctttttc acttgtgtca aattgttttc taacaagcaa
     1681 gctaatcggc tttggtgtga tttgattaat caaaaataca gtgactaaaa taactacgat
     1741 gataactggt actgcaatca ctattcgctt gaaccatttc attcatttac cctctctttc
     1801 ttcgccaaat ccatgtgata atcgtgctaa cagataaaaa tccacctgaa taaatgagaa
     1861 gcgaatgttt cttttctcca gttttcggca acattctttt ttttgcatct gtcttttttt
     1921 gtactttcac tgtcatctga atcggttgcg tatcatcact tgattgatcc tctcgaggtt
     1981 cattctttcc cttattgtct gcattctctg tcggatcttg cttttttggt ggagctactg
     2041 ggattttttt gtaatagaaa ataatagttg gtatcgtctc attaaaaatc ccttcagtcg
     2101 gtccttcaac atttgctaat tcgtatcctt cgaatgtctt ttgctctgtc atataatttt
     2161 ctccataagc tccgtaaagc tggatttcgt cctggtcttt gagtagactt ttattgtttt
     2221 catccacata ctttacgttt atctgatcat ggaacactac tttcaaaaag tccgttacat
     2281 gtttcgtgta ggcagcatgg tcaacttcct gtgcctttcc atgccctgct ccttctacta
     2341 ataaaagatc tttataccct cctgataact catataattt ttgcgcgtta gacacaggta
     2401 ctacatcatc tgctgttcca tggataatca atttaggcaa tgaatctgac attccaatcg
     2461 taggtagagc agcttttgta tccaaatgca tctcctgatc aaaatagtat tgtttcaaaa
     2521 ggcccataaa taaatcttca tgctcttgat ccccaatgat gtctaactga ttttttatgg
     2581 catccggtag tgcgggggct actgtctggc tgtacgtaaa acgtagttgt tcatcaattg
     2641 atgcaaatcc gcaattttca ataattcctt tgacattgac aggaatatct ttcgctaata
     2701 cagacatcat cgtagctgct cccatcgaac caccataaag taaaatcttt tgagatggcc
     2761 gctctcgaac ttcttgattt atccagtaaa ggacgtcatc tgactcatat tgtccatagg
     2821 tcacataatc gcctccgcta ttaccagtcg ctctggaatc aacaaataat aaattatacc
     2881 cggccttata aaaatcattg tattcttctg taacgatccc gttattccaa ttccctcgaa
     2941 atccgccatg gatcatgact gttttatctg aaccttgatc gacataatat gcatatacat
     3001 ccatcatttt gccatcttgt ttgtttagta cagtttgatg aagttgtttt gtatttggat
     3061 agaagcctgt gttttctgta ggcgttgtcc catcatctgt acctccaagc atcgacatca
     3121 acacactgtt ttttgcaaaa gcgatatcgt acaatagata agtagcaatc agatgtttga
     3181 taatctcttt atctacacca taatcattaa cagcaagctc agtgactaat aactctatta
     3241 attttgactt gatgtcttct gttggtccat taagtatttc tgtcatctgt ttaccgaagt
     3301 aattcaaata ttctttacca ttgaaatttt cacctttacc gatatcatca ttttctgtcc
     3361 cattctcttt gtcacttact ttagcagaag aggattcgtc gtttgacatt tttgtagctg
     3421 ttgcttcttc tgtttgttgg ttatctgtgg tagcggtcgt ttcttgatct gtgctagtaa
     3481 cagatgtctc aaccgttgtt tcagtatttg tttctgtttg tgaagtactc gatttcgttt
     3541 catttccagc atcagtagtt tctgtggttt ctacctcccg agattccgat tcggaagaaa
     3601 tgctctctcc atatactgtc cattgcggga cgcttgtact tacagcaaat aagatgacca
     3661 acgctccctt taatacttgt tttgttctca agctattcac tcctttttat gtttctagga
     3721 gaattatatg taaacgcaaa cattttttat acaaaaaata aatggtttat cccataaaaa
     3781 tagaacagga ttattttctc attttacgta ataaagcaga atggataaac cagtatccat
     3841 tctgcttttt ttaaacgaag aaacaactta ttgctccaaa aatgtcagaa atcgtttatc
     3901 ccaaattttc tcgtaaaaag cgattgtttc ctttgctttc ataaaaggat tatcataagt
     3961 tgtcgttgct cctctacaca tcataagctg gtatcctaat ccatgagcaa acttcacagt
     4021 cgtatctata caatactctg cttgtgcccc acataattcg atcgaatgac agtccaattc
     4081 agttaataaa acactcaaat tagtcttata aaatgaattc gcatgggttt ttctcacaaa
     4141 ataactgtgc tcaggaatag ataattccga caatagctcc catggttttg tttgtggcac
     4201 taattgagca tcttcgtgtt gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt
     4261 gattctttta ttcactaatg ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa
     4321 acatacccct ttttgtaaat cgatcactac tattgcgtcg gctgtgtttg acatacttgt
     4381 gccttctttc ttttttcttg atttaatagt atttcttatc tagctagttc cattatacgt
     4441 gacaacattt tcttatttta cacctctaaa tggcactttt cgactatttc ttcatgttat
     4501 tatttcataa atacttagct attggaggaa tgtatgatga ttaaattgta tacaggttct
     4561 ggctgctcgt cttcaagacg cgcaaaacgt tggcttatcg tccatgatat accgtttatc
     4621 gaacagaact taattcgtca accactaacc aaaaatgaat ttatccactt actaaaattg
     4681 acaaacaacg gcgtgaccga aatgctctct actcattcta ttagttataa aaatcttgtt
     4741 cacagaatag atgaactcag tttgagtgaa ctatatattg agattcgtac atccccttct
     4801 ttattgaaaa aaccgattat ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa
     4861 gataaaatca ggatgttcct ttctaaaact aaaagaaagc aagagctcaa cgaactcttg
     4921 ctttctggtc atcttgtcga ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg
     4981 cataaacaat ctgcttttct taattaacga aatccaattt ttccaatatg gatctatttt
     5041 tctcattcct gatttttatg atttcagctt tcagacgcgc ccaatcattt tttgtaggtt
     5101 gtccattcca gtaaaatatc tgtgtattcg tttgatcata tgcctttttg tcgataggat
     5161 aatcgctaat gacaatatca ggtaactgat cagcggatgc actatatttt attgggcgtt
     5221 cagtcataga taatatcctt ttctttgccc ataattcctg tgttttacca tactttgatt
     5281 gaatatagaa tatgacaggt tgttcgtgtt ttataaaaat atccctgact aacatacagt
     5341 attgaaaaat caaccgtctg ttcttttcat aataagtatt aagctctgga attctgcgag
     5401 ctaccccact aaaaaactgt tgcatgattg gatacatcac tggaaatata tgctgaaaat
     5461 ctctgtcttc tgctgatttt ccaaaagcat ccaccttctt ttttgtccca aaaacccttg
     5521 aatacaaatg gatcattgcc aaattgacat atagataatt tttttctgaa gttgtaaaac
     5581 aaatcttgaa gtttttttca aaattttcta caatagacga ttcaatccag ccccaacttt
     5641 cttgtacttt atctggagta tccaacaaat cttcttccaa ataggtgttc attacaccaa
     5701 acataaaata aaggtaagct tgctctgttg gagtgatgtg attcatcgac cagaagtttg
     5761 cttcaaaaaa aggctctagt atgtaatgcg taaactcttc ttttgttatc aatggatgca
     5821 caaatttgtt catctcttct gggacatacg taatcgagtg gccttgtttc attcgttgca
     5881 acgaaattct tagtaggctg atccatttct ttctatctat actacgcgta tatggcgcat
     5941 gtttcgcaaa tttttctaat agttgacgtt tattttttat acttgataat atatcagaat
     6001 gattttcgtc gaatgactgc caatacatca agtgaaaaaa gtatcggata cggtattctt
     6061 ctccttctat cggattacct tttttcaagc tgcaagataa atgatagttt ttcaaaatag
     6121 tattaagttc tcttaatttt ctttgaatcg tacgcaaact tatataattt tttgactaag
     6181 attttctaag gattgataag tacctttgta aagtgaatcc agtaaaatat aaccaatcgt
     6241 tccctctaaa agtatagaat aaactttact cagtaaaaac tgagaagaaa actcgacttt
     6301 tatcatcttg ttttctgaaa gaaaagataa tttatattct ggataccttt cgcaaaacga
     6361 ttctgcttgt tgaatcaccg aattcatctt agccagggtg tagtttagat ctaaagcgat
     6421 atccttcacc acatataatt tctcgtctct ttcataacaa tagttcaaaa attgatatac
     6481 ttgcctttca ttgatatcaa taaattcttc aaacaaagta catactcctt tttatctatt
     6541 atttagactt attccgacag ctacatgaat aacgtacaaa tagattcgtt actgattttt
     6601 cagagatact accatgcgca gtgtgcattg tcaattattc ttgctcttat tttccatttg
     6661 tgttcctgtt atttcctgtt tctcaaatat gttagtaaac aaaaatagat ctggcaaagc
     6721 tacaacttcg ccaggcaata cagcactctc gaatgaaata ttcactcaaa aatctatcgt
     6781 tttattcaat tttagatctg ttgcttttat tctttatttc actcgatgga agagagaacg
     6841 aaaatagctt gctcctacaa tcaaaatgag taacacaccc atccatgtaa ttagcttgtt
     6901 caaggttcta ctaccagttt ctggtaaatc gctttttcta ttatctgttt ctttcgcttt
     6961 ttcttgatga tcaattccac gagaaacaaa atcaggctgt tttagcacac tttttaggtt
     7021 gctccctgca tttctctttt gttccttagt tggtatgata ggtaccaaat caagttcatt
     7081 atttactttt cctgtgctgc tttcgctgct cgtgcttcct gtgctactct cactactcat
     7141 gcttcctgtg ctactttcac tactcgtact ctccgtactg ctttcgctgc tcgtgcttcc
     7201 tgtgctactt tcactactcg tactctccgt actgctttcg ctgctcgtgt tttctgtgct
     7261 actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg tactgctttc
     7321 actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc ttggtttcaa
     7381 attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt tatctttact
     7441 cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa taccagcggt
     7501 ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc cattaccatc
     7561 ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt ttggtttgac
     7621 tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt tcacagtaaa
     7681 tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca ttgctgcaat
     7741 tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt ctatcactct
     7801 ccatataatt accttctatt tgataaagga attatataaa atgaaaatga tgagaacacg
     7861 acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt tgtttacttt
     7921 ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg ctttcttcat
     7981 cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg catagagtaa
     8041 caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt cagccccaaa
     8101 gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa aaccttgttc
     8161 ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc aagtaatatg
     8221 agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga aaaagcaacc
     8281 tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc cgtgcaaaag
     8341 agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa gcatctgatg
     8401 ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa ataaaataaa
     8461 actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg cccccgtcaa
     8521 atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac ctcccgctaa
     8581 atacccccag tcttttcctc gaacagaaac accataatct tctattcttt ttccttcgac
     8641 cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg taccggcaat
     8701 tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat tgacaagaat
     8761 tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa taatcctgat
     8821 catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg tctcgtgaaa
     8881 actatgtaaa tgttctccat catagagaga atagattttt acgaactaag aatatctaca
     8941 gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg tttttaataa
     9001 ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt cagcatgtgc
     9061 aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt ttaaaggtgg
     9121 aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata atcaaacact
     9181 attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag agatttcttc
     9241 aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa actacaagga
     9301 acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta aacagatgct
     9361 ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc tagacactgc
     9421 ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga ctaaagaaga
     9481 agcagataaa gcaaaaaaag aagcaaatga aggagaaaat ttctccaagc ttgttcaagc
     9541 ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg attcaaccaa
     9601 tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg gggaagtttc
     9661 tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc tagtaaaaat
     9721 ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc tagaaaaaat
     9781 tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc gtaaagtgat
     9841 gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca aataaacaaa
     9901 tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat tccttcttat
     9961 ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt tgtaccaaaa
    10021 caaatcactg gatttgttac aatcatgagg atataattta aaaaagtttt ttatttctat
    10081 cacatgaatc aatgcgccat taagtagatt ctttcatgct tccctatttt ttcacttgcc
    10141 gtatcgcttc aaaaacaact atcataaact caaactcgac tgaaaagtta ttttaaaaaa
    10201 gactaaaaat atcccttctg gacttgttct tcttattctg ttatactaga gaaacacgaa
    10261 agatattttt atgcgacact tttcatttgt ttttatgaaa tttattcaaa actggcgtaa
    10321 cttcagagtt acaaggatgt attagaggtt agggaataca tcgtttaaat gagaaataag
    10381 taatagttag taatagtcac ccaaacttta aattaatgaa tattgaatcg tcagatagcc
    10441 tcctcgagtt ttcgagaagg ctatcgttta ttttatggag aaattgattc agaagtaatc
    10501 agcgaccttt tttagttcac caatcgtttc tgcttggaaa gtttgcaccc aatcttcttg
    10561 gaagcccctc actgctttat caatcgaggg catctctaat acttgatcta gtagctcttt
    10621 acctaacgtt atcgcatgag ctccagatcg aatggctgta gtgacttgtt cacaattttt
    10681 gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag tccgcaacga
    10741 atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat aaggtgcaat
    10801 ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa tagctgtacc
    10861 ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg cttgattcac
    10921 cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag catcctctat
    10981 catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc cgattatttt
    11041 tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca gaattgtagg
    11101 atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac tttttgtatt
    11161 tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct tgatcgattt
    11221 taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac attgttcctg
    11281 gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat tcattcaaat
    11341 tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca aaacagagct
    11401 tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa caataatctg
    11461 ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc ctgaatactt
    11521 ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatctct
//
LOCUS       DAYYBH010000083        11445 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000083 DAYYBH010000000
VERSION     DAYYBH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.086"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..53)
                     /locus_tag="ACHAUG_002394"
     CDS             complement(<1..53)
                     /locus_tag="ACHAUG_002394"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8481460.1"
                     /translation="MLVFQMRNVDKTSTVLK"
     gene            complement(102..341)
                     /locus_tag="ACHAUG_002395"
     CDS             complement(102..341)
                     /locus_tag="ACHAUG_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8481461.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            complement(475..1692)
                     /locus_tag="ACHAUG_002396"
                     /pseudo
     CDS             complement(475..1692)
                     /locus_tag="ACHAUG_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2183..3121
                     /locus_tag="ACHAUG_002397"
     CDS             2183..3121
                     /locus_tag="ACHAUG_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8481462.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            3251..3898
                     /locus_tag="ACHAUG_002398"
     CDS             3251..3898
                     /locus_tag="ACHAUG_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFM8481463.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(4591..5529)
                     /locus_tag="ACHAUG_002399"
                     /pseudo
     CDS             complement(4591..5529)
                     /locus_tag="ACHAUG_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(5529..6146)
                     /locus_tag="ACHAUG_002400"
     CDS             complement(5529..6146)
                     /locus_tag="ACHAUG_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8481464.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(6160..6330)
                     /locus_tag="ACHAUG_002401"
     CDS             complement(6160..6330)
                     /locus_tag="ACHAUG_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481465.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(6678..8168)
                     /locus_tag="ACHAUG_002402"
     CDS             complement(6678..8168)
                     /locus_tag="ACHAUG_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8481466.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(8211..8510)
                     /locus_tag="ACHAUG_002403"
     CDS             complement(8211..8510)
                     /locus_tag="ACHAUG_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481467.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8876..9160)
                     /locus_tag="ACHAUG_002404"
     CDS             complement(8876..9160)
                     /locus_tag="ACHAUG_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8481468.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(9679..10164)
                     /gene="vanZ-A"
                     /locus_tag="ACHAUG_002405"
     CDS             complement(9679..10164)
                     /gene="vanZ-A"
                     /locus_tag="ACHAUG_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFM8481469.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(10317..11228)
                     /locus_tag="ACHAUG_002406"
     CDS             complement(10317..11228)
                     /locus_tag="ACHAUG_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFM8481470.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3508 a         2004 c         1742 g         4191 t
ORIGIN      
        1 tgtttcaaaa cagtagatgt tttatctaca ttacgcattt ggaataccaa catgacgaat
       61 ccctccttct taattacaaa tttttagcat ctaatttaac ttcaattcct attatacaaa
      121 attttaagat aatgcactat caacacactc ttaagtttgc ttctaagtct tatttccata
      181 acttctttta cgtttccgcc attctttgct gtttcgattt ttatgatatg gtgcaagtca
      241 gcacgaacac gaaccgtctt atctcccatt atatcttttt ttgcactgat tggtgtatca
      301 tttcgttttt ctttttgtgc gcctaaattt cccacaatca ctcacttctt tctatttctt
      361 cttattctta ttttatcatc aacaatcaca aatcacttgt gatttgtgat aagtgatttg
      421 tgataagtga tttgtgatta atatataaaa gccctcttta aagggctttt atgtttattt
      481 tgagaaagat ataaaatcaa tatatccctt ttccccaatt tttacaacgg cattgtaggg
      541 ctttttctct ttgtttttga ttccttttac cagggtttct tttccctcca gtaattcttt
      601 tacattcgtt ttggttagtt ttttctttct aaaatgttca gctaaagtaa acttacattc
      661 aggataattt gaacaaccat aaaacgattt ttttaataca atattgttgc cacacttagg
      721 acattttcct acaatacttt tttctgcttc tttttctttc tgttcctggt aatcagaaaa
      781 atttagtttt tctatatcgt taggtacagc ttccagtaaa tgaacaatga attttttgat
      841 attcgtaata aagttctctt gattgccttc tcttttaccg atttttttta aatacgtttc
      901 ccatttagcc gtcatttcag cactcgttaa aaggtgctga ctttcaactg cctggcacaa
      961 taattttcct ttttcagtta caacaagctt attcttaatc acttggatat attctttttg
     1021 ttttaacgct tcaataatac ttgctcttgt cgcttctgtt ccaatcccct caacttcttg
     1081 taaaatcttg attgcttctt catcatcaac cgttttattc gccgttttca tagcagttaa
     1141 taatgtaccc tctgtaaaag cttttggcgg ttgcgtttct ttttcaacac tcttaacacc
     1201 aacttcggca cgttcgccaa tgataacgag tggtaacgtt tgaacgtcct ctttttcttc
     1261 tttggtttgt tgtttgaaaa gaattttcca cccttcttgc tttggtgtct ttccgatcga
     1321 ttgaaacagc aaatcagcta ctttcgtttg tatcttggtt tcttcataca agtaatcagg
     1381 taaaaacatg gcaacggtcg ttttaacgac taaagcataa atttttcgtt gtaaatcgtc
     1441 cattttcgct aatgcagatt cggtaggtac ttgttttgtt gggataatgg cgtggtgttc
     1501 ctgtacctta ctaccgtcca cataacgctt tctaggctct gtttgaacca tttcaagatc
     1561 aagtcctaaa aaaccgctat atttgccaaa attcgctttt aaataagcaa attcgttctc
     1621 tgtaataaat ggtgtatctg ttcttggata actcaataat tttgcttcat acagtccttg
     1681 catagctttt aaacgtaaaa ttttgcgttc taagacgttt taaatatatc tgcgtatatt
     1741 tttatgtgtt ttatcttttc gtcggcaaat gcgttaaaat ttgcgaaata aaatagctca
     1801 agggcgaacg cagtgagtgc atttaccctt gatctatttt tttgagtaaa ttagcttttg
     1861 aaaccgacgg aaaggaaaca caaaactttt cagaggcaag gggtttttaa ggggcgcttt
     1921 tagccccttg aatttatttg ctttaagata tatttagttt taaaatagtt ttaacgaaaa
     1981 aaagacataa aaaaagacga gcaacgctac caacgttctc gcccggaata acaaaccaca
     2041 cttgttattc attcgatgat tagtatatca atttttgatt ttgaatacaa aacaaaatgg
     2101 acgaatggat acaggcgaaa tttattttgc gattccactc gtcctttttt cgttccaaaa
     2161 attattttag gagcgataaa aaatgaagaa aattgatgaa aaattagctg cagaaatctc
     2221 actaaaaaaa ccggccgtct ctaatagccg gttaagtaat agccggttaa gtggtcaaac
     2281 tttgggaaaa tctcaacccg cattaagttt tgatgccatg acaatcgttg gaaatttgaa
     2341 caaaactaat gctaaaaagc tatctgactt tatgagtgta gagccacaaa tacgactttg
     2401 ggatatactt caaacaaagt ttaaagctaa ggcacttcaa gaaaaagttt atatcgaata
     2461 tgacaaagta aaagcagata cttgggatag acgtaatatg cgtgttgaat ttaatcccaa
     2521 taaactcaca catgaagaaa tgatttggtt aaaacaaaat attatcgact acatggaaga
     2581 tgacggtttt acaagattag acttagcttt tgattttgaa gatgatttga gcgattacta
     2641 tgcaatgact gataaagcag ttaagaaaac tgttttttat ggtcgtaatg gcaagccaga
     2701 aacaaaatat tttggtgtcc gtgatagtga tagatttatt agaatttata ataaaaaaca
     2761 agaacgtaaa gataacgcag atgttgaagt tgtgtctgaa catttatggc gtgtagaagt
     2821 tgaattaaaa agagatatgg ttgattactg gaatgattgt tttaatgatt tacacatctt
     2881 gaaacctgcg tgggctactt tagaaaaaat taatgagcaa gctatggttt atactttgtt
     2941 gcatgaagaa agtatgtggg gaaagctaag taagaatact aagactaaat ttaaaaaatt
     3001 gattagagaa atatctccaa ttgatttaac ggaattaatg aaatcgactt taaaagcgaa
     3061 cgaaaaacaa ttgcaaaagc agattgattt ttggcaacgt gaatttaggt tttggaagta
     3121 aaataagttt tatttgataa aaattgctaa ttcagtataa ttaatattta cgaggtgaca
     3181 taacgtatga aaaaatcaga ggattattcc tcctaaatat aaaaatttaa aatttaggag
     3241 gaagttatat atgactttta atattattga attagaaaat tgggatagaa aagaatattt
     3301 tgaacactat tttaatcagc aaactactta tagcattact aaagaaattg atattacttt
     3361 gtttaaagat atgataaaaa agaaaggata tgaaatttat ccctctttaa tttatgcaat
     3421 tatggaagtt gtaaataaaa ataaagtgtt tagaacagga attaatagtg agaataaatt
     3481 aggttattgg gataagttaa atcctttgta tacagttttt aataagcaaa ctgaaaaatt
     3541 tactaacatt tggactgaat ctgataaaaa cttcatttct ttttataata attataaaaa
     3601 tgacttgctt gaatataaag ataaagaaga aatgtttcct aaaaaaccga tacctgaaaa
     3661 caccataccg atttcaatga ttccttggat tgattttagt tcatttaatt taaatattgg
     3721 taacaatagc agctttttat tgcctattat tacgataggt aaattttata gtgagaataa
     3781 taaaatttat ataccagttg ctctgcaact tcatcattct gtatgtgatg gttaccatgc
     3841 ttcactattt atgaatgaat ttcaagatat aattcatagg gtagatgatt ggatttagtt
     3901 tttagatttt gaaagtgaat ttaattttat acacgtaagt gatcataaaa tttatgaacg
     3961 tataacaacc acattttttg gttgcttgtg gttttgattt tgaatttggt tttgaactta
     4021 tggactgatt tattcagtcc attttttgtg cttgcacaaa aactagcctc gcagagcaca
     4081 cgcattaatg acttatgaaa cgtagtaaat aagtctagtg tgttatactt tacttggaag
     4141 atgcaccgaa taaaaaatat tgaagaacaa ctagcaaaag attttaaaga gttattttat
     4201 tttaagtctt tataacatga gtgaagcgaa tttttaaatt tcgatagaaa tttttacatc
     4261 aaaaagcccc ctgtcaaaat tgacgaaggg ggttttttgg cgcacgcttt tcgttagaaa
     4321 tatacaagat tgaaaatcgt gtataagtgc gccctttgtt ttgaacttag cacgttacat
     4381 caatttttta aaatgatgta taagtgcgcc cttttaaatt ttgagtgatt atattttttg
     4441 agttagaaaa agggattggg aaaatttccc aaaataattt aaaaaataag caaaaatttt
     4501 cgatagagaa tgtgctattt tttgtcaaag gtgtatacct tgactgtgct tgctgttaca
     4561 ttaagtttat ttttaagtta ttaaaaaaga aatagctttt aaagtttggc tcgctgtcgc
     4621 tttataaagc tgattgactt ttgattgcaa actacttaaa gaaaacaaac tcggactatt
     4681 cgttttcttc tctttggttt gaacatcagc aattatcccc tcttgattgc ctattttagc
     4741 ttgtttagaa gaaacaaaag ctaaaagctc ctcttgggtt ttaaaacgct gtgtggggct
     4801 tagaacgccc ttaaacgacc cttggtttac ttttatacta gcttccacct cgaaaaaagg
     4861 ttctttttta aaattctcta tggcttcctg gcgctgaaaa ataaggtata aggtgggcgt
     4921 ttgaacacgt cctagtgaaa atgtaccttg tacgcccttc tgttgtaaat ttaacgtata
     4981 caaagggctt gcgttcatgc cgatcaacca atcggcaatt tggcgtgttt gcgcttcttg
     5041 ataaaaggga tagtaattca ttccaggttg caaattttga aaaccgcttc ggattacatc
     5101 tttttctaag ctattgatcc atagtctttt aaatgtttta tcttttgaaa aggcatttgc
     5161 tttatggata atcgaccagg cgatattttc accttctctg tcgctatctg ttgcaacaat
     5221 aattgtattt gcctttttga gaagttctgc aacaatttta aactgctttc ccttatcttt
     5281 tgcaacttca aaatcgtatc gatcaggaaa aatcggcaaa gattcaagtt tccaattttg
     5341 ccacttttcg tcataatgac ctggttctgc taattccact aaatgcccaa aaccaaaggt
     5401 gataaacgtt tcatctgtaa atagtgggtc tttgatctca aaataaccgt cttttttggt
     5461 gctttgtttt aaagcacttg cgtaggctaa tgcctggctt ggtttttcag ctaaaataac
     5521 cgtactcatt aactatccct cttttcattg ttttttcttt gatcgactgt cacgttatat
     5581 cttgctcgat accttctaaa cgttcggcga ttgattccag tttgttcttc aacttcttta
     5641 tcggataaac cattcaaaaa caaatcgaaa gcatgcttta aacgtggatc attttcttta
     5701 aatttatgct gacgaccttt gaatttgcct tttttcttag caatttcgat tccttgtgcc
     5761 tgacgttcct taattttttt tcgttctgat tctgcttgat acttgtacaa ttcaatgaca
     5821 aggctattaa tcaaacgcct taaattttca tcttcaatac cattcattga gggtaaattt
     5881 aagacttcca gggttgcccc cttaatttga atttgattca tcaattctgt taattcttta
     5941 ttatttcgtc ctaatcgatc taattcagta acaataacaa tatccccttc acgaatatag
     6001 ttaagcatag cttgtaattg tgggcgttcg accgattgac cgcttaattt gtctgaaaag
     6061 accttagaaa cgccctgtaa cgcttgtaat tgccgatcta agttctgttc tttgctactg
     6121 acacgtgcat aaccaatttt agccattttc aaccaacctc taaaattctc tcggttgcaa
     6181 taaccaatca gcaatatcta ctttttcaat ttcaaattgc ttatcagaaa ttgtcttttc
     6241 gtaagcgata aaatcttgcg catattgttg ctcattaaaa atagccacca cttcgtcatt
     6301 ttctaaaact cgataaataa attttttcat tttactcctc ctattatgcc caacttaaat
     6361 gacctattca ccaagtcaat tatactgcta aaatcatatt aggacaaata ggtatactct
     6421 aatgacctat aaaagataga aaattaaaaa atcaagtgtt cgcttcgctc tcactgcccc
     6481 tcgacgtttt agtagccttt ccctcacttc gttcagtcca agccaactaa aagttttcgg
     6541 gctactctct ccttctcccc ctaataatta attaaaatct tactctgtat atttctgcta
     6601 atcattcgct aaacagcaaa gaaaaaacaa acacgtatca tagatataaa tgtaatggca
     6661 tagtgcgggt tttattttca gcctgtatca tagctaaaca aatcgagttg tgtgtccgtt
     6721 ttagggcgtt ctgctagctt gtttaaagtc tcttgaatga atgtatgctc taagtcaaaa
     6781 gaatttgtca gcgcctttat atagctttct ttttcttctt tttttacttt aatgatcgat
     6841 agcaacaatg atttaacact agcaagttga atgccaccat ttcttcctgg tttaatctta
     6901 aagaaaattt cctgattcgc cttcagtacc ttcagcaatt tatctaatgt ccgttcagga
     6961 atgcctagca cttctctaat ctcttttttg gtcgtcacta aataaggctt gtatacatcg
     7021 cttttttcgc taatataagc cattaaatct tctttccatt ctgacaaatg aacacgttga
     7081 cgttcgcttc tttttttctt gaatttaaac cacccttgac ggacaaataa atctttactg
     7141 gttaaatcac ttgataccca agctttgcaa agaatggtaa tgtattccct attagcccct
     7201 tgatagtttt ctgaataggc acttctaaca attttgatta cttctttttc ttctaagggt
     7261 tgatctaatc gattattaaa ctcaaacata ttatattcgc acgtttcgat tgaatagcct
     7321 gaactaaagt aggctaaaga gagggtaaac atgacgttat tacgccctat taaacccttt
     7381 tctcctgaaa atttcgtttc gtgcaataag agattaaacc agggttcatc tacttgtttt
     7441 ttgccttctg taccgcttaa aaccgttaga cttgaacgag taaagccctt attatctgtt
     7501 tgtttgaaag accaatcttg ccattctttg aaagaataac ggtaattagg atcaaaaaat
     7561 tctacattgt ccgttcttgg tatgcgagca ataccaaaat gattacacgt tagatcaact
     7621 ggcaaagact ttccaaaata ttctcggata ttttgcgaaa ttattttggc tgctttgaca
     7681 gatttaaatt ctgattttga agtcacatag actggcgttt ctaaaacaaa atatgcttga
     7741 taacctttat cagatttgat aatcatagta ggcataaaac ctaaatcaat agcggttgtt
     7801 aaaatatcgc ttgctgaaat agtttctttt gccgtgtgaa tatcaaaatc aataaagaag
     7861 gtattgattt gtcttaaatt gttttcagaa tgtcctttcg tgtatgaacg gttttcgtct
     7921 gcatacgttc cataacgata aacgttgggt gtccaatgtg taaatgtatc ttgattttct
     7981 tgaatcgctt cctcggaagt cagaacaaca ccacgaccgc caatcatgct tgattttgag
     8041 cgatacgcaa aaatagcccc tttgctttta cctggcttgg tagtgattga gcgaatttta
     8101 ctatttttaa atttgtactt taacaagccg tcatgaagca cagtttctac aacaaaaggg
     8161 atattcattc agctgttctc ctttcctata aatcctataa aataggttgt ttaattaact
     8221 tggtttgctt tttcattcaa ctgtttcaat attgcatgtt ttgaaaaaga tttttttcct
     8281 ttataagtca atttttttcc actaatcgaa taaattattt tgttattttc tattaactta
     8341 tatatataat cttccccctc cgaagaaaaa tacttatctg attttgtttc taagtagata
     8401 tttctctttt ctaactcttt cttaaacgtt tctagtgtat agatatttgc taattttctt
     8461 atctccaata aactattttt tatataagtt ttacattcat catgattcat acaaactcca
     8521 ccttctataa atgaatacaa aaaaagcaat caaacgattt ccgattgatt gcttaacaat
     8581 tcttaaattc agtagcttag atacttgaaa actctctgat ttccctatat aatgatagta
     8641 cggttatata ccgtcttcaa acaaagttaa ttaaataact tcttacgagg gaagagttca
     8701 tctgactaac tgataagcgt tggtttggca atcttatcgg gctatgcatt tataaaatgt
     8761 cgtcaaacat tttataaatg tgtcatggct cttttttcgt ttctattcag ttcgttgttt
     8821 cgttatatct agtataccgc ttttaaaaaa aataagcaac gatttcgtgc attattcaca
     8881 cgaagtcatt gcttttttct tcttccattt ctaaatccaa tgttacttgt tctgattctg
     8941 tttctggttc tggttctgtt ggctcatttg ggattaaatc cactactagc gttgagttag
     9001 ttaactttgc aatttgttct agtgttttta tggttggatc tgattttcct gattctattc
     9061 gtgaataatt tgatctactc atttctaatt cttgggagaa tttttctttt gatttggtac
     9121 ctctcatctt ttttaagctt tctctaaatg ctagttccaa gtttctcccc cctctttttg
     9181 tgtaataaaa ttcacgcttt atttaaatta tagcttttaa tatcttaata aacaagtgat
     9241 taatcacttg tgatttgtga ttattccaaa agtaggtaat ttcaccaacc tttttcaaaa
     9301 gtttttatcg acgattattg gactggatcg tgtttcctaa agcgattcta agcgcatgtt
     9361 taattttaaa gtgggtaatt ctctttaaaa cgtcctacaa agattcaaaa aaagcatggc
     9421 tgctagtggg ggtaccgtca ggaaaatgcg gatttacaac gttaaggaaa tttattaaag
     9481 agaccaattc ctcagcacta aaattgataa ttgggctttc tttgttacta tagtgttagg
     9541 taagcttttt actttatcac cggttttccc ctcacttcac acctacgggc gagtttcccc
     9601 acatcccgtt ttccatctaa tccaattcat tcaatatctt tatgtgtatg aaagatcaga
     9661 ttgcttgatt tagacatctt acacgtaatt tattcttaaa tgggtacggt aaacgagcaa
     9721 taatacgagc aaaagtatac ctacaaaaat aataactctg tctaattttt tttgattcat
     9781 atgcttattg cttaaaccat ataatttcag tccaagaaag cctccaacag tatttgtaat
     9841 tacatctgtt atgtctgtcg ctccaatagc gaagataaat tgaattattt caaaagtaag
     9901 acttaaaacc agtacaaaag caaacttagg taaaaatccg atttctttaa aattgacatt
     9961 caaaagcaag ccaaatggaa taaagattat aacattatct atcatctctc tgaaattccc
    10021 agtagcagta aatggagtca agttaagact tctttgatga taattaaata ctgataaaat
    10081 attgtattgt aatttgaata acactaacca gattagtgtc actaaatata aagctagcaa
    10141 tcctctagat aatatttttc ccaatataat ttcctctaat tcttttgttt aagttgtatc
    10201 attagtatag caatgtccta gttaacgtgg gttttaaatt aaaaagctaa cgttacccca
    10261 aaagtgctag tacaataatg ctgttcatta atttcgttgg tttcattccg ccatccttac
    10321 ctccttgaat tagtatgtgt tgatccggga aacactgtca caattacacc gtctatattg
    10381 tttcctgata tctcataacg aagattagtc ggcacatgaa tggtggtatt tttagtaaca
    10441 ggataataaa agatctcata tttttcccca tttacactaa cagaaatggt tttttcttct
    10501 tttaggtaat ccatatattc ctcgagaacg aaattctttt ctttcataat cgcactatgt
    10561 ggtaaaccaa catagcgaat atgccatggt tcatattgaa ttcctgttaa ctctgttttg
    10621 tcctctggat aacgtaaaat gaacccgtat ttccaagcat tttcttctat ccactttcct
    10681 tcaggggctc gttccatttt cgtcaagctt gatcctacat ctagtgataa acctgaatta
    10741 tgctcactat aacctgctgg taaggcatac tcagccccca tttcttggta aagcacactt
    10801 tgctcatcaa agtctcgata gccactatta ataataaaat gactaacgcc accctttaca
    10861 gcatcattga ccatctctga aaatttttgt gctatttctt ttgacatata aatattacta
    10921 tcaagcaacc cgtatccatt tattaattcg tcatgtttag ataaattcac gatatctgac
    10981 ttcacacttt cttggcgaac aggatattta ctattgatta atagcagatt tccttgataa
    11041 acctgttctt ctgtaattgt tttctcttgg gtattttcag aagtcccact attttctaaa
    11101 tgttctttgg gattttgatc ataattttga aattcgactt tttcctgaga aaacagtgct
    11161 tcattaacgt agtcataacc taagtatatt aagaataaca ataacaataa aaaaaacaac
    11221 ttcttcattt tcagtctcct ccttaactat atagttggaa gtatgaaata tttctagaat
    11281 cttcccctta aacggcacaa tctcgtttga gattgtgccg ttttgccccg taaattacag
    11341 tcaattattt cgccctactg gatttactat tatcaccaat gtagatgaat tttcataagt
    11401 gatacccatg ctgaatttaa cgaggctgaa ctgcctgttg acaat
//
LOCUS       DAYYBH010000084        11227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000084 DAYYBH010000000
VERSION     DAYYBH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.087"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..1448
                     /gene="lysA"
                     /locus_tag="ACHAUG_002407"
     CDS             189..1448
                     /gene="lysA"
                     /locus_tag="ACHAUG_002407"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8481471.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1435..2235
                     /gene="dapF"
                     /locus_tag="ACHAUG_002408"
     CDS             1435..2235
                     /gene="dapF"
                     /locus_tag="ACHAUG_002408"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8481472.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2238..3119
                     /gene="dapA"
                     /locus_tag="ACHAUG_002409"
     CDS             2238..3119
                     /gene="dapA"
                     /locus_tag="ACHAUG_002409"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8481473.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3116..3832
                     /gene="dapB"
                     /locus_tag="ACHAUG_002410"
     CDS             3116..3832
                     /gene="dapB"
                     /locus_tag="ACHAUG_002410"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8481474.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3838..4533
                     /gene="dapD"
                     /locus_tag="ACHAUG_002411"
     CDS             3838..4533
                     /gene="dapD"
                     /locus_tag="ACHAUG_002411"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8481475.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4549..5550
                     /locus_tag="ACHAUG_002412"
     CDS             4549..5550
                     /locus_tag="ACHAUG_002412"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8481476.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5547..6908
                     /locus_tag="ACHAUG_002413"
     CDS             5547..6908
                     /locus_tag="ACHAUG_002413"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8481477.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6868..8019
                     /locus_tag="ACHAUG_002414"
     CDS             6868..8019
                     /locus_tag="ACHAUG_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8481478.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7994..9163
                     /locus_tag="ACHAUG_002415"
     CDS             7994..9163
                     /locus_tag="ACHAUG_002415"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8481479.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9338..10717
                     /locus_tag="ACHAUG_002416"
     CDS             9338..10717
                     /locus_tag="ACHAUG_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8481480.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10998..11227
                     /locus_tag="ACHAUG_002417"
                     /pseudo
     CDS             10998..11227
                     /locus_tag="ACHAUG_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3739 a         1955 c         2503 g         3030 t
ORIGIN      
        1 ataaatagct tctaatagtt cgggtatttt gtttttataa ccatttttat tcttattttt
       61 aaaaaaatat ttcgaaatat actagacaat tcagaaaatt ttgtttaata ttattccaat
      121 aaaattcaaa tctttatcaa atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga
      181 atgaaaaaat gacagaattg ataattggcg gagtcacagc aagcacattg gcagaaaagt
      241 ataagacacc tctgtatgtg tatgatgaag aaaagatgag acaaacgatg ctgtcattca
      301 agcgaggctt tgtatcgaaa cgttttgata caaaagtgct ttacgcctct aaagcattcc
      361 aaacgattga gatgctgcat ttagttcgtg aatatggatt cgggctagat gttgtcagtg
      421 gtggggaaat ctataccgct ttgcaagctg attttcctag agatactatc tacttccatg
      481 gtaacaacaa gacgccagag gaactgatct atgcacttga aaatgatttg ttgcatattg
      541 ttgccgataa tttgatggaa gttgaattat tagcagaatt aagccaacgc tatttaaaaa
      601 aaatctgtat catgcttcgg ttgaatgtcg gagtagaagc acatactcat gaatatatcg
      661 tcactgcgca tattgattca aaattcggca tgagctatga aagtaaagag tgccaacaag
      721 cccttcagtt gataaaagaa agcagctatc tagagttaga aggatttcat gctcatatcg
      781 ggtcacaaat ttttgatgtt actgcttggt tagctgaaat cgacaaattg acgaattact
      841 taaccgattt tgaccaacct ctttccttga atcttggcgg agggtttggg attcgttata
      901 cacaaaatga tcagccgatc ccagtcgaag aggcactaaa aaaactgatt gaatatacgg
      961 aagaagcttt ggaaaaacga aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa
     1021 gtattgttgg agaagctggt acaacactat acacgattgg tttcatcaaa aagacaccac
     1081 ataaaaaata ttactttgta gatggcggga tgacagataa tatccgtcca gctctgtatc
     1141 aggcagaata cgattgtgat ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga
     1201 cgattgcagg aaaaatgtgt gaatcaggcg atgtggtaat taaagaagct tatttgccga
     1261 aagcagaatc gggtgatctt ttagctgttt attccacggg agcttatgga tactcgatga
     1321 gcagcaacta caatcgagcg acacgaccag cagttgtttt tgtcaataat ggccaatcaa
     1381 gactgattgt tcgcagacaa gcattttctg atctactaag gggggaagtt tcctatgatc
     1441 cttcataaat ataatggctg cggaaatgat tttatcttat tagattacgc agaagacaca
     1501 gattatagtc aattagccgc ttctttatgc acaaaagaaa aatatgatat agacggattg
     1561 atcgccgtaa aaatcgatcc gttggaaatg atctattaca acaaagatgg cagccgcgcg
     1621 ccgatgtgcg gaaatggcat tcgctgtttt gctcgatacg taaatgatca gaacatgatg
     1681 aatgaacagg aattcaacgt agagacatta gcaggcacaa gaaaaattac ggtgttggat
     1741 ccctcacctt tttattgcag cgtagatatg ggagtgccag atgattctcc ggaaaaaatc
     1801 aaagctaatc aaacttatcc aataaaaaac caggaaatag agatcaatgg tgaaaaagta
     1861 acgatcacta gtttattttt aggaacgatt cataccgttg tttttgttga aaatgcgcgt
     1921 gaggaagtct ggagaaaaag aggtgaactg atctgtcatc atcctttgtt caaagaaaaa
     1981 accaatgtca actttgtaga agtagtcaat gaacaagaat tgatcgtccg tacctatgaa
     2041 agaggagtag gttggacact tgcttgcggt acaggttgct gtgcctctta tgtggttgct
     2101 aaggaaactg ggaaaatttc agcagatcag gtagttgttc acttggaaca aggagacttg
     2161 atgatctctg gtgaggatgc gatacaaatg gctggcccag cagttcatga atggacaaga
     2221 atattggagg aatgaagatg ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg
     2281 aagagggatc agtagattat gctggtttgg aaaaattgat ccaattccat ttagatgaac
     2341 aaacagatgg attattagtt ttaggtacaa caggagaatc ttctacgctc acacaatctg
     2401 aggaggagca gatccttcaa ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg
     2461 caggagctgg aacgaataat acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt
     2521 tgggggcaga cgcacttttg gtcatcaccc cttactacaa caaaacaagt gatgcaggac
     2581 tagctgctca ttttacagca attgcagaag cctcaccgat tccgcttatt ttgtataatg
     2641 ttccttctag aacaggcatg tcgattccaa tccatgtatt agtaaattta gcagaacatc
     2701 ctaatatcat tggtttgaaa gaagcctcag gagatatggc ttatgtgatg gatgccgctc
     2761 ggttgattgg agaagaattc ttcctttaca gtggaaatga tgatttgatc ctaccagtaa
     2821 tgagtgtagg aggaagcggt gtaataagtg tatgggcgaa tatccaacct aaaatagtcc
     2881 atgaattagt aaaagatacg caggacggaa gatggcagca agcaaaagaa aagcaattaa
     2941 atgcacttga actgatccat gccttgttca gtgagaccaa tcctattcct gtcaaagcag
     3001 ccatgtcatt attagactta ccttccggac ctctacgcct gcctttagtt tctctttcaa
     3061 aagagaaaaa gaaacaatta gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa
     3121 aatcggactg attggcagtg gacagatggg aaatagagtc gaagaagttg cacaagaaag
     3181 aggagatacg gtccttcttt tacggacggc acctaaagaa gcaactacgc ttgcttttac
     3241 ggaactgcca gaactgctta ttgatttttc tcatccagat aatttagaga aaatcctttc
     3301 gtatagctta tcatatcaac ttccgttagt cattggcacg acaggttata cacaatcaca
     3361 gttccaagaa ataaaagaac atgccaaaca tgtacctgtg atgtatagtg ctaatttttc
     3421 gttcggcatc atggtgatga atcaactaat caagcaagca gctgcaatgt tgcaaggctg
     3481 gcagatcgaa ctgattgaaa aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc
     3541 aaatatgctg cttcaaacaa tccaagaagt gcaaaagctg cagcccgtat acaactggca
     3601 agggaaaaaa agagaagaaa accaaattgg tgtacacagt attcgtgcag gttctttacc
     3661 tggtgaacat gatgtattgt ttgctgctac tgatgaaata tttacgatca aacatgaatc
     3721 tttttctaat cgaatctttg ctgcaggcgc agtagaagca gcggactggt tgaaagatca
     3781 gtcaccaggt tattacaaat tagaagattt attaatggac aaaggggtgt aaaaaacgtg
     3841 gaagctcaag aaatcatccg ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc
     3901 tatctgaatg taaccgagcc aatcgaattt aaagaaagta aagtttttgg tgaaggaagc
     3961 agcttcgttg tttttggtga ctatgagacg attgctccgg ttttagaagc tgaatcagaa
     4021 aaaatcattg aaatcgaaat cgaaactgct gcaagataca gcgcagttcc tttgctagat
     4081 attaaaaaaa ttaatgctcg tatcgaacca ggtgctatta tcagagatca agtgtcaatc
     4141 ggcaacaatg ccgtgatcat gatgggagcg attatcaata ttggtgcagt aatcggtgaa
     4201 aacacgatga ttgatatggg tgctgtctta ggtggacgag cgacagtggg caaaaactgc
     4261 catattggtg caggagctgt cttagcaggt gtgatcgaac cagcatcggc caaaccggtt
     4321 atcgtagaag acggcgtact ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc
     4381 ggaaaagatg cagtcgtggc agcaggagca gtggttttag aagatgtggc agcagaaact
     4441 gttgtcggtg gaatacctgc acgtgtatta aaaataagtg atgatcagac aaaagaaaat
     4501 acagcattga ttgctgcgtt acgcgaatta tagaggagat ggagaaaaat gcgcgaaata
     4561 gatacctgta ttgtgggggc aaccggatta gtcggccaaa cgatgctcaa agtgttagaa
     4621 gaatatgatt ttccagtagg acgattgaaa ttattagctt caaaacgttc agccggtaaa
     4681 gtttgtgtat tcaagaaaaa gaaacacact gtacaggaat tgacagaaac ttcatttgaa
     4741 ggatatgatt tagcgctatt ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc
     4801 gcacgaagtc agggactatt tgtcatagat aattcctcag catggcggga agattcggat
     4861 attccattaa tcgttccaga ggtcaatctg gaagactata cattgaatcg gctgattgca
     4921 aatccaaatt gttcaaccat tcaagcggta cttccgctta aagctttaca agaagccttt
     4981 ggtatccagc gagtcaatta ttcgacctat caagcagttt ctggttccgg gcaaaaaggc
     5041 attgaagatc ttcatcgtac gcaaaatgac gaggaaccag ttttgtatcc ttataatatt
     5101 agccaaacgg tgattccgga aatcgatcag cctttggaaa atgggtatac aaaagaagaa
     5161 cagaaaatga taaatgagac ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca
     5221 acctgcgtac gtgtgccaat cgaaaatgga catggagtct ctgtggcagt tcaactgcaa
     5281 aaagagttta cgacggaaga aattagagag tgtctcagcc agtttccagg gattcaatta
     5341 gaagatgatc tagcagcaca taaatatccg acgagcattc ttgctagagg aacagatatg
     5401 gtttatgttg ggaggatcag aaaagacact tctttagaga acggattgtt gttctatacg
     5461 actgccgata acatacgcaa aggagccgct gcgaatgccg tacagattgc tgcagcttta
     5521 atgcagaaaa tagcggaggt ggcaagatga gtaatacgct gaaagtagcg aaattcggcg
     5581 gaacatcgat gtctcatgaa gagcaattca aaaaagtccg tacaattgtt ctttcagatc
     5641 ccaatcgcca agtgatcgtt gtttctgctt taggcaaacg tacgtcagat gatgacaaag
     5701 taacagactt actttatttg atttatgctt accttcagca tcatgttgat tggacacccc
     5761 tttggaaaag gatttgtgat cgtttcattc aagtaagaga tgttcttgcc ctgcagttac
     5821 ctattgaaga tgaactagca cgcatacaga aacaactgga agacgaaaag ataactgaag
     5881 attatttagt aagtagaggt gaatatctga ctgctcaact gatggctgag tatcttggtt
     5941 accagtttat tgacgcaaaa gacctgattt tctttgacta tgatgggaaa atcgatcagg
     6001 aaaaaacagc acaggaaatc acaaaagcat ttaaaagata tccgaaaatg gttgttccag
     6061 ggttttacgg ggcgaaccct gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca
     6121 ttaccggcgc gatactggcg aagatgttgc aagcggaaag ttatgagaat tggacggatg
     6181 tttcaggaat catgatggct gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa
     6241 tctcttttag agaactgaga gagatggctt atatgggggc taacgttctt catgaagcag
     6301 ctgttttccc agttcaagag gctgatatcc ccatccaaat aaaaaatacc aatgatcctt
     6361 tcgctcaagg gacaaaaata tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa
     6421 tagctggacg caaggatttt ctttccatca ctttattcaa acgtcatatg tccgatgaaa
     6481 tcgggtttat ctggaaagca atgagcattt ttgctaaaca tgagatatca atcgaacata
     6541 ttccttcagg gatcgacaat atcggagtga tcgtctcagc agaagcgatt gccgataagt
     6601 tatttctcat cacgaaagaa ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg
     6661 aagacttagc tttgatctct gtcgttgggg gtccgcataa agaattgatt ggattatctg
     6721 ggaaagtgtt atcgatattg aacaaattag aaattcgcac atcgatctta tcacaaggag
     6781 cacaggagtt gaatctgatc atcggtgtac cgaataatca atatgaaaca gtagtgaagg
     6841 gtatctatga agggatggtg aatacggatg ctgaaagaat atcgaatggc actccatcaa
     6901 attcctgaaa tcgggtttca tgaattcaag acaaaaaaat atttatatga acaagtaaaa
     6961 aacagcgagg ccgttatcca tgaaatcggc gaaacaggac tactccttta ttttgataat
     7021 cagcaagaag aaacgattgc ttttcgaaca gatatcgatg cactgccagt caaagaagaa
     7081 acgaatctct cttttgcttc taagcatgaa ggttatatgc atgcatgtgg tcatgacgga
     7141 catatggcga tgcttttagg gttgactgac tttataacta aacacattga ggatcttatt
     7201 tataatgtag tactgatcta tcagccatca gaagaaattt ccggtggcgc acaaagcgtt
     7261 attcagtcag gattgttgga taaatatgaa gtacaagcaa tatttggctt tcatctatgg
     7321 cctggactag ctaagggaga agttttctct cgaccaggac ctttgatggc acaaagcagt
     7381 gaaacagaca ttatcattaa aggccagtct gcacatattg cttctagtga aaaaggaatc
     7441 gatagtctgg aggcagctgt acgatttttg aagaatgttt atgattttga ccggcaatta
     7501 ccggcagagt ggaatcatct aatgaaattt ggccaaatcc aaggtggaac gatccgaaat
     7561 gttttagctg gagaagttat tgtatccgga agtatccgtt cttacagttc agatatgcaa
     7621 aattatttga agaggggatt agaccattta gcagaacagt ttcactctac agctactatt
     7681 tcttttcgct ataatgacgg ctatccagct gtaatcaacc atagaggctt atatgcagca
     7741 ctgttagatt caaacaaact acatgaatta gatgctccag ttcttcaagc cgaagatttt
     7801 ggcatatata cgcaacaata tccatgtgta tttttctttt taggtgtcgg tgatacccct
     7861 gctttacatg atccgacgtt cgattttgat atggatgttt tagaaaatgg tgtagaatgg
     7921 tacaaaacga ttctttttac agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag
     7981 gagcgagtaa ataatgatga atttatccaa tcgatttagt cctaggcttt ctagaataga
     8041 agtatcaaaa atacgacaat tcgatcagca aatatcgtcg ataccagatg tgatcaaact
     8101 caccctggga gaaccggatt ttccaactcc tgaacatgtc aagcaagcag gaatagcagc
     8161 gattgaagaa gacttttctc actataccgg aatgagagga ttagaggaac tgcgggaagc
     8221 ggcttgtata tttcagcaac agcgttatgg attaacttat gatccgcaaa cggaagtttt
     8281 gacgacagtt ggtgcaacgg aagcgattgc ctctgcgttg ttatctgttt tggaagaagg
     8341 agacaaggtt cttattccag cgcctgctta ttcaggatat cagccattag tggaactagc
     8401 tggtgctgaa ttgatcccga tagatacttc tgacacaggg tttgtctgtc agccggaaca
     8461 atttgaacga gcatttgaac aatatggatc agctgtcaaa gcagtcattt taaactatcc
     8521 taacaatcct actgggacga cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt
     8581 aaaaaaatat ccggtatttg ttataagtga tgaagtatat gctgaattga cttatagtgg
     8641 aacgcatatg tccatagcta cttatcttcc agaacaaaca atcgtgataa gcgggttatc
     8701 caagtcacat gcgatgactg gttggcgagt gggctttatt tttgctcaaa aagcccttat
     8761 tgacgaacta attaaagtcc atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa
     8821 agctgcggct gaagcgctaa taaatggggt gaaagatagc gagaatatga aagagcaata
     8881 tcaaacacga agagactatc tggtccagca attggcgcct ttaggatttg aagtgaccca
     8941 gccaaacggt gcattttact tgttttgcaa actcccagct gccattcaaa ctgactcttg
     9001 gcagttttgt gtagaattag cggaacaggc aaaagttgcg tgtatcccag gaattgcatt
     9061 tggcccagaa ggagaaggtt atatccggat ttcttatgct agcagcatgg aaaaattaca
     9121 tgaggcctgc aaacggatca aagacttttt aacagaacag tgattcatag attttatcaa
     9181 aaaaacaatc gttaattttg cggtatattc tgcaaggaga cgattgtttt ttaaatttag
     9241 ttcaaatgaa cgcgtttctt ttgattcata actaatgtaa tgttaagttt aaattgttta
     9301 gaatgatttt aaaagaatat tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat
     9361 tgtaggtgca tctcatggag ggcatcaatc gatcttggaa ctattgacac gatacggtga
     9421 aaatgtagat atcacgctat ttgaagcagg agactatatt tccttcatgt catgtggtat
     9481 ggagctgtat ttagaagaca aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga
     9541 aaactttccg caatccaatg ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc
     9601 cgataaaaaa acagtaacgg ttactagcgc atcagataac gaaacaaaag aatatcctta
     9661 tgacaaattg attttgagtt ctggtgtcaa accaaacaat ctgccagtac caggagcaga
     9721 tctagaaaat gtctacttga tgcgtggata caattgggca accaaaatca aagaaaaatt
     9781 aaatgatccg gctattaaaa aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc
     9841 agcagaagca agccgaaaag ctgggaaaga agtcgtattg cttgatgtga tcgatagacc
     9901 tttaggcact tatttagatg ctgagatgac agatatttta gaacaacacc taaatgaaaa
     9961 cggtattgaa gttttgacaa atgcaaaaat cgaagctttt actggtaacg gaaaagttga
    10021 ggcaatcaaa acaagtgaaa aagaaatccc agcagatcta gtcattcaag ctgccggtgt
    10081 gaaggcaaat acagaatggt taaaagggat cgtcgactta gatgaacgtg gctggatcca
    10141 aacaaatgaa tacttgcaaa cgaatcttcc agatgtgtat gcagtaggag atgctacact
    10201 tgcttattct atcccagcac gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag
    10261 agaagcacgt tatgtagtaa aacatctttt tgaagaagta ccaaacaaac cgttcggcgg
    10321 agtcgtaggt tcctctgcat taagtgtctt tgactatcat tttgcagcaa gcggattgaa
    10381 tagctttaca gcagcaaaag caggagtaga tgtacgagtg tcttattatg aagatacgtt
    10441 acgaccaaaa tatgtaccag aagaacaagg aaatccaaaa gtgtccatcc aattattctc
    10501 ggatccaatg acccatcaat tgctaggagg agcagtatta tctacctatg atgttacagg
    10561 tcaaggaaat gtattggcac tagcgatcca aaacaaactg actttagaag aactagcaga
    10621 agctgacttc ttcttccagc caggctttga ccgacaatgg agcatgttga atctagccgc
    10681 tcaacaagcg ttaggagaag aaccatttgt agaataagaa aacgaaatat ttgaaaagag
    10741 gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaaa cagcttctta
    10801 tgtttttaac ttggctggac ttattaccgc agatgcaagc ttttgaacac cgtttatttt
    10861 caaaaaaggg actgtgacac gatttgtgta ccgaccccca aaagttagac caaaaaatct
    10921 aacttttgga ggtcggtatt ttttatggtt aaatatactt ttgaacttaa attaaagatc
    10981 gttcatgatt atttggatgg caaaggcgat tggaccgtaa aaatccagat caagagattg
    11041 aagcggaaat gcttaaaatt cgggagaagc acaaggatta tggctgttta cggatgacca
    11101 atgaactaag aaaccgcggt ttctctatca ataaaaagaa agttcagcgc ttgatcaaga
    11161 agctaggaat aaaagtaacg gcatacacgc gtaaatcacg tcgctacaat tcttatcgag
    11221 gaaaagt
//
LOCUS       DAYYBH010000085        11197 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000085 DAYYBH010000000
VERSION     DAYYBH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.088"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACHAUG_002418"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACHAUG_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8481481.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACHAUG_002419"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACHAUG_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8481482.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACHAUG_002420"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACHAUG_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8481483.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACHAUG_002421"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACHAUG_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8481484.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACHAUG_002422"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACHAUG_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8481485.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACHAUG_002423"
     CDS             complement(6343..7062)
                     /locus_tag="ACHAUG_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8481486.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACHAUG_002424"
     CDS             complement(7059..8372)
                     /locus_tag="ACHAUG_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8481487.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACHAUG_002425"
     CDS             complement(8453..8773)
                     /locus_tag="ACHAUG_002425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8481488.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACHAUG_002426"
     CDS             complement(8881..9168)
                     /locus_tag="ACHAUG_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481489.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACHAUG_002427"
     CDS             9295..9939
                     /locus_tag="ACHAUG_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8481490.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACHAUG_002428"
     CDS             complement(9998..10705)
                     /locus_tag="ACHAUG_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8481491.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACHAUG_002429"
     CDS             complement(10925..11095)
                     /locus_tag="ACHAUG_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481492.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1956 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DAYYBH010000086        11154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000086 DAYYBH010000000
VERSION     DAYYBH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.089"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..587
                     /locus_tag="ACHAUG_002430"
                     /pseudo
     CDS             67..587
                     /locus_tag="ACHAUG_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(585..1304)
                     /locus_tag="ACHAUG_002431"
                     /pseudo
     CDS             complement(585..1304)
                     /locus_tag="ACHAUG_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1329..1514)
                     /locus_tag="ACHAUG_002432"
     CDS             complement(1329..1514)
                     /locus_tag="ACHAUG_002432"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8481493.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1741..3014
                     /locus_tag="ACHAUG_002433"
                     /pseudo
     CDS             1741..3014
                     /locus_tag="ACHAUG_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3121..4152)
                     /locus_tag="ACHAUG_002434"
     CDS             complement(3121..4152)
                     /locus_tag="ACHAUG_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8481494.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4159..4989)
                     /locus_tag="ACHAUG_002435"
     CDS             complement(4159..4989)
                     /locus_tag="ACHAUG_002435"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8481495.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4986..5801)
                     /locus_tag="ACHAUG_002436"
     CDS             complement(4986..5801)
                     /locus_tag="ACHAUG_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8481496.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5798..6622)
                     /locus_tag="ACHAUG_002437"
     CDS             complement(5798..6622)
                     /locus_tag="ACHAUG_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFM8481497.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6612..7712)
                     /locus_tag="ACHAUG_002438"
     CDS             complement(6612..7712)
                     /locus_tag="ACHAUG_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8481498.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7890..8675
                     /locus_tag="ACHAUG_002439"
     CDS             7890..8675
                     /locus_tag="ACHAUG_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8481499.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8708..9091
                     /locus_tag="ACHAUG_002440"
     CDS             8708..9091
                     /locus_tag="ACHAUG_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8481500.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9167..9298
                     /locus_tag="ACHAUG_002441"
     CDS             9167..9298
                     /locus_tag="ACHAUG_002441"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8481501.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9267..9503
                     /locus_tag="ACHAUG_002442"
     CDS             9267..9503
                     /locus_tag="ACHAUG_002442"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8481502.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            9515..9601
                     /locus_tag="ACHAUG_002443"
                     /pseudo
     CDS             9515..9601
                     /locus_tag="ACHAUG_002443"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9581..10552)
                     /locus_tag="ACHAUG_002444"
     CDS             complement(9581..10552)
                     /locus_tag="ACHAUG_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8481503.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3333 a         2577 c         2130 g         3114 t
ORIGIN      
        1 aaaaagagac tcacttttgg taaaatttag tcgaccaaaa catcacccta ccaaaggagt
       61 cgctctatgg ataatcatac tagaaaatta ctcggattaa cagataaaca tttatttttt
      121 gatgaagaat ggctgattga aaaagaatat aaaggcgttc aagcgcagtt tattaaaggg
      181 agattggatg actcaccttc ccattgtgaa cactgtggat cgattcgtat tatccgcaac
      241 ggttcctata cgacacgtac tcagatgttg aaagtcaaag aaaagttgac tattttagaa
      301 ttaaaacgta cccgttttct atgctatgat tgtgggcaga ccttttctgc gaaaacggac
      361 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcgattct catagaactg
      421 tacgaaaatc aatcacgtaa tctgaaaatc agaagaagta ccgtcggctc aagcgatatt
      481 ggaaactctt actcaaagat tccacgacat tggagccgct caaacggcat tatcatcgac
      541 tgtttaaacg accaattagt caaacagaaa tcgtcgatga accgttgtgc acaatggtgt
      601 cctgaacgta cagcgacacc ttccatatcc agagcagtcg ccacatcacg gggatggatg
      661 ccatccaaat taaaagcaac cacgccagta tgatgttcag gatcttgcgg tccgtaaatg
      721 gtgataccgc cgatcgcttg taatttaggt aaaacgtagg caaccagttc ttgctcataa
      781 cgatgaatat tctccatacc aatctcattc aagtaatcga ttgctcctgc aatgttcgga
      841 gtaccagctt caaatttcca tggcagctct ttccatgaac tatcgtaaag attgacaaaa
      901 tctatcattt caccgccgaa ttcgactggc tccatttgtt ctaacaattg acgtttgcca
      961 tataatacac cgattcctgt cggcccgcac attttatgcc cgctaaaggc gaaaaaatct
     1021 gcatccaacg cttgtacatc aactgtcata tgcggtgctg cttgggcacc gactggacta
     1081 tcctcaacta caacagcctg ttcaaccctc agccgtttca agcacaacaa ataaccttct
     1141 ggatctggtt tgccgacctg aagatcatcg atcgtcacaa aggaatcaat tttttcctta
     1201 atcatcggat gtgcttcaaa aaaatagtct agctcccgca gttcagaatt gagactactc
     1261 cgattttcca gtgctgctga tccgctgctc aaaatgttca gcatccactg gtctcccaac
     1321 cgcttgactt atcccttcat aaaaggacag ttctgctgcc gtcccaacac cttgttgata
     1381 gtctgcaaaa ggaattttat agccgaactg ctccagtaaa taggcttcag taaatagtga
     1441 tgaatactct cgtaaaacat ccattcactg tcaatcatcg tcccatcaaa atcaaaaata
     1501 agtgctttgg tcatcttctt tcccccttac ccaaaacaag caaaagctgt gtcctgctct
     1561 ctctacagta tcagcataaa aactgccgtc tgaaaagtca aaggtgaaag aaatgtggcg
     1621 ctataatttc taggactgat agtaaatcat tctatcagtc cttttgagca taaaaaaaca
     1681 aaacatacag aagctccgtt agaattgaat caccacaaca caatcaaagg agcgttctgt
     1741 atgcctgatc ttcatcttat caaactatta ctaggaatca aagatggaaa cattcaaatc
     1801 actcatgtcg agaagaaaaa aatacattca atcaacagta tcgttgtttc tgccacgctc
     1861 attaaaacga ttcagccctg tcctcagtgt aaacaggtga atcaacatgg gctgatcgtt
     1921 aagaacggaa agaaaaaatc gctggtcctt ctcaataagt gtgcgaatca acggacttat
     1981 cttgctttgg caaagcaacg gtacctctgt cgtggctgtc acacctattt tacggcgaat
     2041 acctacattg ttgaaaaaaa ctgttttatc gccaaacaag tacattataa aatattggag
     2101 gaattgactg aaaaacaagc aatgacgacg atttctaaac attgcggcgt ttcttggtca
     2161 accgtttcgc gaacactcgc ttctctgctc cccatgacaa acgtgaaaat aaattggttg
     2221 cctcgctgtt cattggtaga tgaatttcgt tcattgaaaa atcaagtcgg tccctattct
     2281 ttttcttgta tggatggcga tactgggaaa ttactggata ttcttccttc acgtaagaag
     2341 aaagatctca tttcttattt tatgcaattt gaacgacgtg cacgtttaaa cgtaaaaata
     2401 ctcgtaacag atatgaacgc ctcttatgct ttgctgataa aagagtgctt tcctcatgcc
     2461 aaactcgttg tggatcgctt tcacatcgtc aagcacctca ttcgcagttt taaggatatt
     2521 tgactatgag tcatgaaaag cttcgaccga aacgatccaa tacaagccaa acactatcga
     2581 caagtgaagg ctttatctcg acttctgatc aaacgacagg acacgcttgt ttatgataaa
     2641 tggacaaaat ggcgtaattt tggttgggcc tatttaaccg aaagtgaagt cgtagaacgt
     2701 ctactttcta cttctgatga actacgaata gcttatgctt actatcaaga aattcttcaa
     2761 gctttttatg acaaagaagc ggacatcttc tttcagcttg taaaaacaat gcccaagtcg
     2821 gttccaagag aactgcatca tataaaaaaa gcgtttataa actatgaatc cggcattcgt
     2881 ttagccttag agttgcctta ctcaaatggc aaaatagaaa atctgcatac ccacatcaaa
     2941 gcattgaaac gaatagctta cgggttagga tgagaaccag aattttctta atcaatcatc
     3001 tgatcactta ttaaacataa aaaaacatct aaagcagaaa atcaattcta ccttagatgt
     3061 tcttatatca ctctatcagt cctatttgac aaagagccag aaatgtttgc cgactataca
     3121 ctagctttgt tgtgcaaaat gctgagtatc aatttccaca gggattgcta gaatatcttt
     3181 cagctgaatg tataaggcat cgctaatttt ctcttcccga tcgttcaacc gatccactaa
     3241 aatttctttg ccctccagcg tttgaaaaac ataccgttga atgttgccta aaataaatga
     3301 ttccttccat agaaccggaa tcttaacccc ctgaggaacc ggttcaaatt gaatcagctc
     3361 cggtcttaaa taataaatgc agcgattgcc cttagcggaa aactcgtggg tatactgatt
     3421 cagctcatac cattcgaaac gattatagtt gccgataaat tccgcaacaa atcggctctg
     3481 aggattctga tacaattcct tcgaggtgga aacctgagcc acccgcccag cttccatcac
     3541 aaaaatccga tcactgattc gcatcgcctc ttcttggtca tgagtcacaa aaatcatcgt
     3601 catccctagc gattgctgga tagcgcgcaa atcccgctgc agctgttttc taattcgggc
     3661 atccaaagca cttaatggct catccagcag caggactttc ggttcagtga ccaatgagcg
     3721 agccagtgcc acccgttgtc gttgaccgcc agaaagaaac tggacatttt tatctgcctg
     3781 ctccgtcata tccaccaatg atagcatctc aaaaaccttt tcctgaatca cctcaggact
     3841 cagcttcttt accttcaaac caaaggctac attttcaaaa acagtcatag ttggaaacaa
     3901 tgcatactgt tgaaaaacaa agccgatatt gcgatccttc gtaggcttat cggcaatatt
     3961 ctcattgtcc agcacaatct caccgctctt tggttgttcc aatcccgtga tacagcgcag
     4021 caacgtagac tttccgcacc cgctgggacc tagtaaagta accagctcac ccttttcaat
     4081 ctgaatttca tcaatagcta aaatctgttt ttcatcaaaa aacagattga gatcatgtat
     4141 ttctaaaaac attttccgtc acaactcctt taagggcttc tttttcgact gcttctcttc
     4201 cctcgtctgc ttaagggaca gctgttgctt ttgtcgataa gtcagcataa aggtagttaa
     4261 agcaattccc accataaaca gcaaatacaa aaccatcaca gcgctagcca aatggccgtt
     4321 ttcgttcatc cgccgcagca tatagatgcg taccgtctga aatcgaccgc caatcaacag
     4381 gttcgtcagc atgtactccc caaaaaccga agaaaaaatc atcagagaaa ctagggtcac
     4441 accgatacgg atgtttggaa acagcacctg gataataatc gtagacatcg gggcgcccaa
     4501 ggtttctgct gcatccacta attccttcag attaattaag cgcatctggt tgttaagact
     4561 aagatagaca atcggcaacg ccgtaataaa caatgcacct accaacacta caaacattgg
     4621 gataccagtt tcagcatacg ttcgaagcaa agccgttacc aaaatcactc ctggtaaagc
     4681 atacggcagc aggagcagct tttccagcca acgattcaaa cgagggaaat acaaatgaat
     4741 ccaaataatg gtgggaatca tcaacaacag aatcgtcacc aagaccacac ctgctaataa
     4801 aagtgagcga cccaccgcag caataaattc cctgtccgtc agcagctgtt ggaaccattg
     4861 caaggtaaaa tcactaggta aaagcgattt tgaccattta gtggagctgg catacaaaat
     4921 cgttccaata atcggcagca agtaatacag gacaaagcag aacaaaatcg gttttattaa
     4981 ctttttcacg tctgcaccac cttgcctttc ttttgaataa tcaacattgc cgttaccagc
     5041 aggacaccta aagcgactgc caacgcactg cctaaattag gcttggcgaa cacatctccc
     5101 gacactaaag ccgcaattcg aatcgttaaa agatttacat tactgctggt cagggcatac
     5161 gctgtttcat aggtccccat gccattagca aataagatga caaaggtgct ggcaatcgat
     5221 ggtcgcaaaa aaggcaaagc caccttgaac caagcatacg ctgatgaagc gcccagcaat
     5281 tgtgcggact ccagccattc ctttttcact tccttcatgc tgggatacaa aaacaagacc
     5341 cctaacggaa tttggaaata ggtatacgtg atggtcaatc cccaccaaga atacaaattg
     5401 aagtccgcta gaaatggcaa aatcattttc agcacaccta cattccccaa caaaataata
     5461 aaagagaatg ccagcggaat cccagcaaaa ttagctgcca aattggaaac aaaggtgatt
     5521 ttctcctgca atttagtcga tagattcatt aagcataaac ataaaataaa cgtcccgatt
     5581 aacccaatca cactggagct gacagcaatc agtacgctgt taacaaaggc ctgtgtgtaa
     5641 tatttgttgc tgaaaatatc actgtaattg gccaatgtcc actggttagt taaatcactg
     5701 tgcaagctgc acagaaccat aaaaagcagc ggcagcagta aaaatgcaca gaagataaag
     5761 gaggcaggaa ccagaaccaa gcgttttttc atctctttca tcccctcatc aattcttgaa
     5821 tgtcctgcat tttctctgac ggtgcaattc ctaataacca gcaaaacaat ggagccgcca
     5881 gcagctgtgg tacagtttgt tccttcagct tttggcgttt atttgaaatc aaataaaaag
     5941 gaacttcacg atgtgcagcc aagctgccgc catgcagtcc gtgcgcatcc atcccgtgat
     6001 ccgcagtcac cacgacttga taaccttttt gcagccattc aggaaggtaa atagccaata
     6061 atctgtccgc ctgattaacc gctgctgcat attcatgaga atcggcggta aatttgtgac
     6121 cgacatcatc aatattcatc gaatgtacga ccaaaaagtc tggctgatac tgattcacta
     6181 aataatgggc atccgcaaat aaatgcgaat cagggtaagt gtcctcacta taaaagatgc
     6241 catgctgaat cagtcgctcc tcctgatgct ggatgcgatc aaccaaacga ttataggggg
     6301 cttgattgta caactcgctg taccagtggt aagcggcggc tcctgttttt ccgccagctt
     6361 ccttaactaa ttcaaacagc gaccactctt tggatcgctg agcatggtaa ttactataga
     6421 tgccattttc ataggctgga acgccggtca tcagtacttc gtataacgga cgtgaattgc
     6481 tgggcagctc actttttacc ttcaataagc ttgcttgctg gtgctcaact aaatgattca
     6541 tgaacccaag ctgggcgacg gctgtgtcgt agcgacagcc gtctagtata actacaatca
     6601 gtttgtcaag tctactttgc ataggtcagt acctccgcct gccaaagctc tggaagctct
     6661 acagaggtct tgttccaagc ttcattgtct tcaactggca ccgccgcttc ataagcatcc
     6721 tccggcagta atttttcagc tacttcttta ggcagctcta ccttgcgaat aggtcgcgca
     6781 tagccctttg ccaaattgat ttgcccttca tcagataaaa tatattcccg tgctagcttt
     6841 gccgcattgg gatgaggggc atttttattg attaaggttg tatacccgct aatgatggag
     6901 ccgtctgttg gcacagttac ctcaaaacgc tcggagtcaa tttggtcacg ataatttagt
     6961 ccattgaagt cccaaacaac tgccacatcg atttcacctt tttcaagatt tgctacgctg
     7021 gcatcaacgg tggacaaacg gcctttttct gccatttttt tgtagtaatt cagacctggc
     7081 tgaatatcct tttcgtcacc tccgttggca atcgccgcag ccaatacagc aaattgtgct
     7141 tggttggcaa tcgtcacatc ccccacgcta acttgatagt caccctcttg cagctccttc
     7201 cagctagtcg gtgcttgctt cacattctct ttatcggtga taaaggaaat ggttcccgta
     7261 tagcttaaca accaatcccc atcatcatcc tttgcccaat ccggaatttc ctcccaatag
     7321 ttggtcttat aaggtaaggt taactctttg ctttcagcca acggaccgaa gctgataccg
     7381 acatcaccaa tatcggcggt tccatttttt ccttctgatt caaacttagc caattcttct
     7441 gcactggaca tatccgtatc cacgtgactt aagccgtatt ctttttcaag gtcgttccaa
     7501 gtgcctaccc aatttgccca agtatccggc attcccaagc tggcaatctc tccttcttcc
     7561 ttggcttttt ctataatcgt atctaaagag tcgtctgccg cagccgattg cttcgttgct
     7621 tcacctgagc cgcagcctgt cagcaggata cttactgttg ctgcacaaaa taaaccgata
     7681 gtcatgttac gaattttttc attcattttc attcattctt ctcccctttc gataattcca
     7741 ttctaacaaa gctttgtaat gagagaatca gtttcttgaa aaggtttcat gaacaatgta
     7801 aaggttgtat atggtttgta aatttcgacg aattcccgtt aaccttctaa agcttttgcg
     7861 gtatgatagt agaaaaaagg aggctcccta tgcagtatta tgatcagcat ctccacacgt
     7921 atttttcctt tgattcagag gaaaaatttg aaaactattt agcctatcag ccggagtttt
     7981 tcgtttctac ggatcacttt gatttaaaca atccatctac cggaaaagat gacattccgg
     8041 attatccagc ctatataaac aaactggact ctttaaaaga aacctattcc accaaatttc
     8101 tgcgaggtat cgagattggc gttgttccag gacaagaaga gcggatcgtg gattacctcg
     8161 ctcagcatcc ttatgattta aagctggtaa gtattcatca aaatggccgc tttgattata
     8221 tggacgatgt ggttttaacc aaagatccca ttcaaactac caaggaatat tttgatcaga
     8281 tgttgcaggt cttgacggat tttccagaag gagatatttt gactcacttt gattatggat
     8341 tacgccgctt caccttcaca gcggaagaat tcgcggagca ttttgaggct cagctgctaa
     8401 cgattttcaa aaaggttgtt gaacgagaat tggcgatgga gttaaatgcc aagagcttct
     8461 tgaaatacgg caacgaagac ctttaccgtt atgccgttcc tttgtatcaa agtgtcggcg
     8521 gaaagctctt taccttaggc tcggatgctc atgttgccga agattatcag ctgggcttta
     8581 aaaaaatgag tcagctgttg gcagactgtc aggtagaaaa gttgctagtc attcaaggaa
     8641 aagaacgccg cctgacgccg ctgccgattt tgtgattcct atataataga agaaagtggt
     8701 gaaaaaaatg agcaacctgt gggactacaa tcaagaggca cccatccatt atttaattgc
     8761 gcgacattgg gatgcgttga aaattgaggc ggtttgccga tccttgttag cagccgttcc
     8821 aaaacaacaa ctggaaaatt ttttggtggc tgacagcttg cagcgggaaa aagttcaggc
     8881 ttattttgca gcctttaaag atcagccttt ggaatatttg catgcgcagt ttcatctttt
     8941 ttatcaagta gccgccccag atgactacaa cgatttacga ggacagctgc aattgacctt
     9001 tcaagcggat gaaaccgcct ataccgtttt attgggaatg gctcgccttg gtgatcaggc
     9061 gaaggtcgag tggcggattt tcgatatttg atggcaggat caaggcgttt cagttgcaaa
     9121 ccttcctctc tttcaattag aaaaaccgca gcaggaagaa gtttgtgtgc tggtcaccga
     9181 tgctggtgaa attcgtctcc gcttatttcc tgaccaagct cccctggctg tagccaactg
     9241 gcgctcccta gctgagcaag gcgtttatga caacacacct ttcggctcgt gtcattaagg
     9301 aatttgttat tcaaggtggc gctttggatg gttctggtga tgaagctgtt tccagctatg
     9361 agggcttttt cgctgatgaa gtgcatcgcg gactttatca ttttaatggc gccttggcct
     9421 taggggacca cggcccccac accaacggca accagttttt tattgtgcaa aacacaaagg
     9481 cgcaggctga cctgctaatg taaagcaagc atacttggag cttggaggac taccggagct
     9541 ggatggtcgt tatactgttt ttggacaggt ataccaaggg ttagaaatcg tagcagcgat
     9601 cgcagctcag ccgacaaaca gtgaagatgt accgattgag ccagtgttca ttcgacaaat
     9661 taacttcgaa aaaacatcgt aaaaagacac tcactgactt cagcaaaagc tctattcttg
     9721 agctggctgc agctagtgag tgtctttcat ttatccataa gataaagctt ggattcaata
     9781 aataaataaa tgttttggtt aaagctgtga ccagcaaatg aggaaccgtg cgagccagtt
     9841 gatctgccaa tgcaaacatt tctttaggtg aaggcgctgg cgtgtctgcc aacggatact
     9901 ccagtcccaa tgcttggtaa ccgtgcatac catagcagtg gtacggtagt aattctaacg
     9961 gtacgaaccg atcaagatct gccacaaaac aagcaatcgt cagcagctcg gtcaggttca
    10021 tattgatacc tggaaccaca ggaactcgaa ttcgcacttc gcagttagga tactcaatta
    10081 aaaagcgacg aatattcttc aaaatttgtt ggttatctaa gccggtccat tgtttgtgga
    10141 gcttgctggt aaacattttt acatccacaa acaatgtatc cacataggga gctacctttt
    10201 ggatttcatt ataagcacca caaaagctgg tttccagtac ggtattgatt ccaatgtatt
    10261 tcgatttttg caaaatttct ttagcaaaat ccgcctgaac cagtgcctcg cctccgctaa
    10321 tggtcactcc gccatccgag tgaaagtaaa aaacagcatc cttctcaatc tcatccatta
    10381 cctcttcagc ggtcatggtc tggccaaatc ctttttcaat ctgcttgctt tgtgattcgg
    10441 gaatcgaaca ccaggcacag cgtaatgggc agccctttaa ataaacaacg gtccgtaaac
    10501 cagcaccatc atgtagagaa ccctgttcta ttcttaatac tgttccttcc atatagattt
    10561 ttgaattaca cactagttta aactaaaaca cacaaggaaa aacttaccat ttaatcataa
    10621 aattagctca tataaatata ctaggatgta aaatagactg gtcaaaatct ggtcaaaaaa
    10681 gaggactaat acaggtagtc ctcttttttg ttattttaat attctaaatc attttttatt
    10741 ggtttattcc cttattcgat gtagactcaa atcactatat cactagatat caaacggcgt
    10801 catacggaat caaaaacatc aaatttgacc tgttaaatgg tcattgaata acattcaaaa
    10861 tcaataaaac ggagtcagac acggagtcaa aaacgctgag agtaaaaaat atatacagcc
    10921 ttgtaggtaa agtaccacag ggctgtatat tttttatttc agattacctt aaatgattcc
    10981 atttactttt tatctcattt tttcctacta gttagtatca aatactctcc tattttctca
    11041 ataaactatt tttggataca tggtatctga aacagctaaa accttgtcat tacagcaaaa
    11101 aatcacgtta tccagactgt agacaaagtc cattttatgg atgatgtcta cagt
//
LOCUS       DAYYBH010000087        10812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000087 DAYYBH010000000
VERSION     DAYYBH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.090"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACHAUG_002445"
     CDS             <1..135
                     /locus_tag="ACHAUG_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8481504.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            185..808
                     /locus_tag="ACHAUG_002446"
     CDS             185..808
                     /locus_tag="ACHAUG_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481505.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1258..2307
                     /gene="trpX"
                     /locus_tag="ACHAUG_002447"
     CDS             1258..2307
                     /gene="trpX"
                     /locus_tag="ACHAUG_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8481506.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2304..3185
                     /locus_tag="ACHAUG_002448"
     CDS             2304..3185
                     /locus_tag="ACHAUG_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8481507.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3188..3958
                     /locus_tag="ACHAUG_002449"
     CDS             3188..3958
                     /locus_tag="ACHAUG_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8481508.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4237..5091
                     /gene="aroE"
                     /locus_tag="ACHAUG_002450"
     CDS             4237..5091
                     /gene="aroE"
                     /locus_tag="ACHAUG_002450"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8481509.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5269..6432
                     /locus_tag="ACHAUG_002451"
     CDS             5269..6432
                     /locus_tag="ACHAUG_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8481510.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6549..6959
                     /locus_tag="ACHAUG_002452"
     CDS             6549..6959
                     /locus_tag="ACHAUG_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8481511.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            7133..9361
                     /gene="topB"
                     /locus_tag="ACHAUG_002453"
     CDS             7133..9361
                     /gene="topB"
                     /locus_tag="ACHAUG_002453"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8481512.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9532..9816
                     /locus_tag="ACHAUG_002454"
     CDS             9532..9816
                     /locus_tag="ACHAUG_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481513.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9976..10212
                     /locus_tag="ACHAUG_002455"
     CDS             9976..10212
                     /locus_tag="ACHAUG_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8481514.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10209..10607
                     /locus_tag="ACHAUG_002456"
     CDS             10209..10607
                     /locus_tag="ACHAUG_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8481515.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3781 a         1803 c         2272 g         2956 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat ataataaaaa ataggagaaa aagtagaaaa
      181 gaaggtgttt gttatttggg aagtagttaa atcgagagta caagaaatga atgaacattt
      241 ctcagtgtca ttcgcagaaa atccaagtaa gtattttcga cgtcttgttt ttctgctcat
      301 cttatcattg ctgttaacag ttgcaatgtt tttattgccg gaaaaaatcc gagaagagca
      361 agtaaatagt ctcgtttatt cgtccatacc aaccagcaaa ttgaaacctt ttgattacag
      421 caaagctgat cagaaaatca aacaaagccg ggcgatatcg gtgatgttca gcaaaccgaa
      481 cgggaaaacc tatcaggaca tgcttgatgt ttttaatgat cctgagctta tggaagaatt
      541 gaatcgaccg atcttttatt atccgatcgt ttatgatgta catgaactgg aacaaaaata
      601 taacatacga acggatgaag tgacatttat tttctttgat ggaggaaaag aagcaaatcg
      661 gataactgcg ggtaagggag gaataactga ttttgataag gagttgatcc ctgaactgaa
      721 tcgacttccg ttagccaata tcaaacaatt agaagaagaa ttggatagaa cgggagtaac
      781 ttcccaaaat tccgagattg ctaattgaga cattggcgcg atgaaacggt gggtctatat
      841 agcgctattt ttaattatca gaacaagtga tgtttgatct tcaaaataac aaataaaaaa
      901 ttaaaaaata tagttgacga aattcaaaaa ttcgattaga cttaaatcaa ttcttaagga
      961 aagggtgacc tgtaatgaac ttatcaaaac gtatttcagc agaaaacaaa aaaaattggc
     1021 gcagctggcg tagataagtt gtattaatgg tgagactttc ccatagatac aactttttag
     1081 agtgcatttc taaagcttgt atctatgcta gacatgaaca ttgactgccg catagattag
     1141 aatctatgcg gttttttgtg taaagacaat gaccgctttg gccattgtct ttttttgtag
     1201 actggatact tgcctgatta taataaataa aaagattgac tttgcaggag gaaaaaaatg
     1261 aaaaacaaac gattaataac agttgtagta attattttag tttatttagc aggtgcattt
     1321 atttatggaa aagcctcagg cgaaaaagaa agtacttcga cgaaccagac aaaaaaaaca
     1381 acagttgttg gggtattgca atacgtgagc catccggcat tagatgagat ttatcgagga
     1441 atcaaagatg gtctgaaaga agaaggatta aaagaaggag aaaatcttga gatcaaattt
     1501 caaaatggac aagccgatca gagcaagcta gccacaatga gtcaacagct agtacaatct
     1561 gatccagatg ccttgattgg aatcgcaaca cctgccgctc aatcattagc aaacacgact
     1621 tcatcgatcc cagttgtgtt aggtgctgta acggatcctg tgggggctgg attagtcgct
     1681 tctttagaga aacctggtgg taatgtaaca ggtgtttctg atcagccgcc tgttacttca
     1741 caaatcaagc ttgcttctga tttgttgcca aatgcaaaaa aagtcggaat cttgtatgct
     1801 tcttcagagg ataattcaaa ataccaagta gcacaagcag aagaagctgc aaaaaaagaa
     1861 gggttagaat cagtaaaata tgcggtgcct tccactaatg agattgcaca aacggttcaa
     1921 gtgatgagcc gacaagtcga ttttatctat gttccattag ataataccat tgccaatgcg
     1981 atgcaaacag tggtaaaaga agcaaacaaa gcaaatatcc ccgtcattcc atctgtggat
     2041 acaatggtgg aacaaggtgg actagcaaca atcgggatca atcaatatca attagggctt
     2101 caaagcggta agatggcggc agaacttgcg agtggaaaag aaaaaccgga aacgacgcct
     2161 gtttatacat ttgatcaagg ggatacagtg atcaatcaat ctcaagctga tcgcttagga
     2221 atcattgttc cccaatcgat gaaggaaaaa gccaaaatca ttacagatga ctcgcagcaa
     2281 gaaacatcta aggaggatga caaatgatcg tatcagctat ttcacaaggc atgctttggg
     2341 caatattagg attagggatt ttcatgactt atcggatctt agactttcct gatatgacga
     2401 cagaagggtc cttcccatta ggtgctgctg tctgtgtgac agctattact catggggtag
     2461 cgccgattgt agctacatta ttaggcgtag gtgctggcat gatagcagga ctcgttacgg
     2521 ggttacttta tacaaaagga aagataccgg ttattttagc agggatttta gtgatgtcag
     2581 gattgaactc agttattttg tttgtgatgc gaacaccaaa tctttcttta ttgaatcatc
     2641 cgattctaca agacagcttc catcgctttg ggttacctga ttactttgac acagtccttc
     2701 ttggcttcat ctctttgggc atcttgattg ctgtactgct attcttcttc aatacagatt
     2761 tgggtcaagg ctatattgcg acaggagaca acgaaacgat ggctcggtcg ttagggataa
     2821 aaaccgatcg tatgaaaatt ttaggtttaa ctttgtcgaa cggtgtcatt gcattatccg
     2881 gtgcgctgat tgcccagaac gacggatatg cagatgtaaa taaagggatt ggcgtaatcg
     2941 tcatcggtct tgcttccatc atcatcggtg aagtgatctt ccaagaattg acgctagcag
     3001 aaagattgat tgctattgtt gtcggcagta tcatttatca gctattgatt ttagtagtaa
     3061 tcaaactagg gtttgacacg acttatctga agttgttctc cgctatcatc ttagctattt
     3121 gtctgatgat cccgcaattg aagaaagcac tgaacttaaa aacgggtttt gagaaggagg
     3181 catagtaatg actgatatat tgacaatcaa aaatggcaga aaagtcgtaa acaatggaat
     3241 gaatgaagag aaagtactat tggatgaaat taaccttaca atcagagaag gggacttcat
     3301 cactgtcctt ggcgggaatg gtgctggaaa aagcacactg ttcaatacga tcgctggtac
     3361 gcttcaactc agtcaaggat cgatctcttt taaagatcag ctgatcacaa aagattcaga
     3421 agaaaaaaga gcacgattcc tttctcgagt tttccaagac cccaagatgg gaacggctcc
     3481 tcggatgaca gtagcagaaa atttgctact tgcacaaaaa agaggtcaaa aacggaaatt
     3541 ccgtttacgt aacttgaaaa atcaaaaaga agattttttc agactatgtc aagaagtcgg
     3601 taatggccta gagcaacact tggatactcc agcgggtgaa ttatctggtg gtcaacgcca
     3661 agcattaagc ttgctgatgg cgacgatcac agctcctgag ttactattat tagatgaaca
     3721 tactgcagct ttagatccta aaacagctaa agcattaatg cgattaacaa atcagcggat
     3781 caatgaacaa aaattgactt gtctcatgat cacgcatcga atggaagacg ccttgaaata
     3841 cggaaatcgt ctgatcgtac tccaaaaagg gaaaatcgtt caagatattt ctgaaaaaga
     3901 aaagcaacat ttgacaatgg gcgacctttt gcagttcttt gaagaagtag tggattgatt
     3961 tcgacagaaa aattcctttc agtagaagga cttgaaaact cattgacatc ttcctttgat
     4021 tcatcctata cttaaaacaa taaacaatta aacatatttg ggtgttttgt aggatcacgt
     4081 agtcaatgat tttaacaatt aaatagaata ttctttgctc tgacaagtaa acgatgagaa
     4141 aagaatttgc tactttgcca cctagacgtt tcgtactttt tcgtatcaaa cgctaagtgg
     4201 ctttttttat tgaagtaaga acaaaggagg aataagatga tttctggaca aaccaaatta
     4261 gctgggctct ttgctgatcc ggcttctcac agtttatccc cattgatgca caatactgct
     4321 tttgaagcaa acaacatcga tgcggtgtat ttagctttcc aagtaaatcc aatgaatttg
     4381 aagcaagcag tcgaaagtat tcgaactttt gatatgttag gtgtaaatct ttcgatgccg
     4441 aataaaacag cggttattcc ttatctggat gaggtaagcc aggaagcgca attgatcgga
     4501 gcagtgaata cgatcgtgca cgaaaagggt cgtttgattg gctataatac cgatggtgcg
     4561 ggcttcatgc aatccgtgca agaagctggg atttcgatca aaaagaaaaa aatgactcta
     4621 ctgggagctg gaggtgcagc aaaggcagtc actgttcaag ctgcgttaga cggcgctgag
     4681 gaaatcgtag tttataaacg aaacaaccaa acattcgctc aagtaaaaga aacattcgaa
     4741 caactcagct atcgaacttc ttgtaagata aaagttcgtg actttgccga tacgcatcaa
     4801 ttaagaaaag acttgaaaga aagctgtctt ctagtcaatg caacagatat cgggatggga
     4861 aagaaaatca atcagtcacc gctcttagat ataagcatga tgcatgagaa tctagcggtt
     4921 tttgatttga tttatgcacc tagagaaaca cgtttattaa aagaagcaaa aaaagtagga
     4981 gcaaaaggat acaatggact tggcatgttg atctaccaag gagcaatcgc gtttgaatta
     5041 tggacaaagg aaaagatgcc gatccatgtg attgaaaagc tgtttgatta aaaaaggata
     5101 atagataaag atcttttatt ctcgcataaa cggtctcaag aaaagtaatc ctttatgtat
     5161 cgaagcattt atttcgttat tttttattaa attatcaaaa aatgataggc ttttgtcgat
     5221 tggttatcgt atcctttatt tatcagtaag aaacgaggag gaaccacaat gagtcggatg
     5281 gatcgctata aggacatcca taagaaggcg aaacctctga agcgggatac ggggagtatt
     5341 tttgctagaa gagaacgaaa acgaacgcgt atggaaaatg aggaagatgc tacccgtatt
     5401 tatgaaagag agcagcattc atttgaacat ttgaaccaat acgagcaaca gatatacaga
     5461 gatgcaccaa gagcagaaga acaacaaaaa aataaaaaat ttttcaatcg gcaaaagaag
     5521 aacaaaccgt taaaacctcg aagaaagcga tcgtggaaaa aaatcattct atcgattttg
     5581 cttgttttga tcctttattc aggtatctca ttttttattg gtaaatatgt agctgaacat
     5641 gacacaagct tagaggcagc gacttcagaa acctttcatg ggcaaaaagc gacgaatggt
     5701 gcgcataata ttttgatcct tggaagtgat acacgcggag aagatgccgg gcgagccgat
     5761 acgatcatgg tcttacagtt ggatggtcct tcccataagc cgaaactgat ttcttttatg
     5821 cgtgacagct tcgttacgat cccaggggta ggacaaaata agatcaattc agcttatgca
     5881 tacggaggag ccgatctagt gcgtcaaact ttagtagaaa attttggaat cgattgtcaa
     5941 tactatgcga aagtcgattt caaatcgttt gaaaaagtga tcgatgcctt attcatgaac
     6001 ggagtgaaga tcgatgcaga aaaagatctg aatctggatg gtgtagatat taaaaaaggg
     6061 gtccagaaga tggatggaca tgttcttttg cagtacgcac gtttccgaat ggatgaacaa
     6121 ggagactttg gccgtgtcag acgccaacag caagtaatga acgctatttt cagccaattg
     6181 aagaatccat tgaatctgat ccgtgcacct tatgcagctg gtaaagcttt aggatataca
     6241 tccacagatg tcactgcttg gttcttgacg aaaaacctct tttcgattgc acgaggagct
     6301 ggtggcattg atcgaataag tgtgccagta gacggttcgt ggaattatgg gcaaagtgat
     6361 tatgctggca gtattcttga aattgataat caaatgaatc aatctgctat tacagatttt
     6421 ttgaataaat gatcataaaa aagatcaaga agaagctgcg caaggctttt gtcatgaatc
     6481 aatcaaacga ttcactcgta aaaaacgctt tcttatggta gatttaacca agagaggagc
     6541 tgtttacatt ggatatctat ctgaacttca aaaatgagtc gcgtgaagcc atcgcttttt
     6601 atgaaaaagt ttttcagact gaagcatcag gtatcaccac atacggtgaa atgcctggaa
     6661 tagaacaaat gaacttgcca gaagaagcaa aaaatctaat cacgaatgca cggctgaaca
     6721 tttatgatac attagttatg tttgcagata cgccagaagg aattggtcct gaattagttg
     6781 tgggaaataa tgtttcttta gtcatccaga caagaaatga agaagaaacg aagcgcctat
     6841 ttgaaagctt agctgagggt ggtaagattg agatgccact ggatgctgtt ccgtgggctc
     6901 agttgtatgg gatggtaact gatcgatttg gcatcaactg gcagattaac caagagtgac
     6961 agctaaagat gaatattttc tggaaacatc aagcagcaaa cggatcatcg tttgtttgct
     7021 tgatgttttt gtgtctattt cgactatacg gtatcttttt tttattgcca gatgcgaaaa
     7081 tatgttccat aatagagaag tccgcaaaaa attaagtaac ggaggtcaga agatgaagac
     7141 ggtgatacta gctgaaaaac cttcacaagc aaaagcctat gccgaaacct tccaacagca
     7201 aaagcggcat gaagggtatt atgaaatcag cgatccattg tttgctggtg aagtaaccat
     7261 cacttatggt tttggacatt tggtagatat ggttgctcca ggagcttatg aagagcgttg
     7321 ggcaaaatgg tctttagaaa atttgcctat tttcccagaa accttccgtt acgaagttcc
     7381 aatggacaaa cgtgcacaat tttcgatcgt aaaacgagaa ttgcagtcag ccgataccat
     7441 cattattgca acagatggtg atcgagaagg tgaagccatc gcgtggtcga ttattcgaca
     7501 agcaaacgca ttttctaagg aaaagaacta tttacgttta tggatcaatt ctcttgaaaa
     7561 agaagcaatc tatgaaggtt ttaagaatct ccaacctgga aagaactatt ttccaaaata
     7621 taaagaagcg caagcaaggc agaacgctga ttggctgatt ggaatgaatg gaagtccatt
     7681 atatagcttg ctacttcaac gaaaagggat tgatggcagt ttttctcttg gtcgtgtaca
     7741 aacaccgaca ctctatatga tttatcagct gcaagaagaa atcaaacact tcaaaaaaga
     7801 aacgtacttt gaaggagaag gtttaattac gacaaacaaa gggcagtttt ctggaaaaat
     7861 cgtaccaaaa aaagcattta aaacccaaga agagttaacg aaggaaatag aaaaacttgg
     7921 agcgcattta ggtaagcaaa acggacggat tcttgaagtt acgaaaaagg aaaagaggtt
     7981 gcatagtcca cgtttatttt cgctatctag tttgcagaca aagatgaatc agctaatgaa
     8041 agcaagtgcc aaagctacac ttgatgctgc tcaaggattg tacgaaagca agtttctaag
     8101 ctatccaaga acggattcct tttacattac agaaaatgag catcagtatc tggttaaaca
     8161 tctgcaaaat tataaaaaat ttttaggaat agaagaagta cagacaactg agatcaaagc
     8221 aaagaaacga tatgtcgatg cgaaaaaagt tcaagaacat catgcaatta ttccgacaaa
     8281 gacgattccg actcctgcac gctttgcgaa gctttccaaa ttacagcaag caatctattt
     8341 gcaagttttg cgcactactg tggctatgtt tgctgcagat tatatctacg aagagacgac
     8401 cgtaatgacg ggcgtacagc aattaaagct aaaaagcatc ggaaagatcc cattacagca
     8461 aggttggcag gaaatcttac gcatcaagtc aaaaaagaaa gaagctcaga ctcttcctcc
     8521 tatcgtagaa aatgaagaag cagaagtaga actgaaaagt atggagaaag aaacacaacc
     8581 tccaaaaccc tataccgaag gaacgttgat cacagcaatg aaaactgcgg gaaaaacgct
     8641 ggataatgat gaagcgcaag aaattctcaa agaagtagaa ggaataggga cagaagctac
     8701 acgagccaat atcattgaaa atcttaagca gaagcattac atcgaagtca ataaaaacga
     8761 aataactgtt acgccaaaag gaatcactct ttgtaaggca gtagccaatg aaccattact
     8821 gacgagcgca gaaatgactg cgcgctggga agggtacttg agaaaaattg gtaaacaaga
     8881 ggggacacca tctgtttttt tagagaatat caaaaaattc atcctacatt tattggcaga
     8941 agttcctaat caaatcgaca aggtagactt ttcagatgag attagcggga cacgtcagat
     9001 aaaagcgatg gagaagaaaa acgatcaatt aggaagatgt ccaaaatgta aaaagggaat
     9061 agtcatgctt tatcctaaaa tcgctacatg cttgaatccg gaatgcgact tcaaattgtg
     9121 gccaacagta gctaaaaaga agctgactaa aacgaatctc agagatttgc tttcaaaagg
     9181 aaagacaagt aaagttgtca aaggttttac tggtaaaaaa gggaaattcg atgcagtttt
     9241 agtattaaaa gaagatatga caatcggttt ttcttttcca tggatcgaaa acagtggaac
     9301 aaaagcagtt gatggaaaca aagaaacatc aagaaaaaat aatgaaaacg aagtagagta
     9361 aatgaaaaca atgaaatagc ggaatattta ttccgaaaag tgagaatata aataccaata
     9421 aaactaaaaa atatcagtga atcaagcaaa aacgttgggg taggctgatt ttatatagat
     9481 agcaaggagt ttttttattt gaaaaaagtg cattcatttt taaattatct tatgggagca
     9541 atcacgattg tggctgtgta ccaagcattt ctaaagtatc ctgattacgg gacgaagttg
     9601 ttgtcattga cgcacaatca aacagcatat tcaccaacgt tgattttatc gactttgact
     9661 gcaggagcta aaagcgctac ttattttttt cataaaacga caagcatgct aaagcgaaag
     9721 aaacagcgaa aacaattgtt ggtggtggta gcggctatga tcattattta ttttctacca
     9781 gttattacac aagtatttca tcaatatttt ggttaagaat acgagagttt gtgacaaaag
     9841 tcatgtcaca aacttttttc tttgtcattt tttcgtaaga aaaagcaaaa taaaagtata
     9901 tacattgatt aaaagtatat acttggatat aatgtactta ctatagaatg agaatggtat
     9961 agagggtggc gaaatatgaa aaaaatgaaa aagtataaaa ttagaaaatc tggcaatagc
    10021 gatgtaacaa ctatcccacc agaagtaaaa gaattcatgg gtgtgcagac aggtgacgca
    10081 atcagttatg tttttcaatc tgatggttct gtacgaatga tcaaagcaca agaagaacct
    10141 gatattgatt cattagttga ttcgatcatg aatcaatatg aagacgcgtt aaaagatttg
    10201 gttgatttat gaggtatctt tcgactaaag aaattatcaa aatcaacgcg aaagttattt
    10261 tacgtcactc gccaggcgaa atgatcggtg taaaagatgc aaatgcatta gatatggccg
    10321 tcaaacagcc ttcacaagca gtattcaatc aagaactcta tcctgaaatc tatgaaaaag
    10381 cggcgatttt agcaattaat ttagctaaaa aacatccttt tcataatggc aataaacgaa
    10441 ctgctcttgt tgccatgttg ttgtttcttc aattgaataa ttgtccagtt gctttttctc
    10501 gtcaagaagc tgttgatttt atcataatga ttaccactag ttcattggat ttcgattcac
    10561 taaagtccaa gataaccagt tatttacgac aaaccgcaat aaaatgaacg aattaaaaac
    10621 tttatttctc aattagatca gcatgtcatt aaggaatatg ctgatttttt tatgtttaat
    10681 catacaaaat agttttttca taaaaatatg gaaaatatgg attaattttg aaataattga
    10741 tttaaaagga atacaaaaga gtgaatagta ggaaatagtg gtttagaact agtgggctga
    10801 ttttttatat at
//
LOCUS       DAYYBH010000088        10668 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000088 DAYYBH010000000
VERSION     DAYYBH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.091"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(211..1059)
                     /locus_tag="ACHAUG_002457"
     CDS             complement(211..1059)
                     /locus_tag="ACHAUG_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8481516.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1223..2125
                     /gene="gnd"
                     /locus_tag="ACHAUG_002458"
     CDS             1223..2125
                     /gene="gnd"
                     /locus_tag="ACHAUG_002458"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8481517.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2127..3128
                     /locus_tag="ACHAUG_002459"
     CDS             2127..3128
                     /locus_tag="ACHAUG_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8481518.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3125..3409
                     /locus_tag="ACHAUG_002460"
     CDS             3125..3409
                     /locus_tag="ACHAUG_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8481519.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3442..4239
                     /locus_tag="ACHAUG_002461"
     CDS             3442..4239
                     /locus_tag="ACHAUG_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8481520.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4253..5086
                     /locus_tag="ACHAUG_002462"
     CDS             4253..5086
                     /locus_tag="ACHAUG_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8481521.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5104..5961
                     /locus_tag="ACHAUG_002463"
     CDS             5104..5961
                     /locus_tag="ACHAUG_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8481522.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5983..6492
                     /locus_tag="ACHAUG_002464"
     CDS             5983..6492
                     /locus_tag="ACHAUG_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8481523.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6529..6930
                     /locus_tag="ACHAUG_002465"
     CDS             6529..6930
                     /locus_tag="ACHAUG_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481524.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7042..7395
                     /locus_tag="ACHAUG_002466"
     CDS             7042..7395
                     /locus_tag="ACHAUG_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481525.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7429..7872
                     /locus_tag="ACHAUG_002467"
     CDS             7429..7872
                     /locus_tag="ACHAUG_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8481526.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7889..8974
                     /gene="uxuA"
                     /locus_tag="ACHAUG_002468"
     CDS             7889..8974
                     /gene="uxuA"
                     /locus_tag="ACHAUG_002468"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8481527.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8967..9680
                     /locus_tag="ACHAUG_002469"
     CDS             8967..9680
                     /locus_tag="ACHAUG_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8481528.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9887..10036)
                     /locus_tag="ACHAUG_002470"
     CDS             complement(9887..10036)
                     /locus_tag="ACHAUG_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481529.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10432..10632
                     /locus_tag="ACHAUG_002471"
     CDS             10432..10632
                     /locus_tag="ACHAUG_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8481530.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3559 a         1691 c         2312 g         3106 t
ORIGIN      
        1 ttttactact gtatactaac tttgttatcg catctaacaa gtaactcgaa taataccaat
       61 acaaccttct ccaaagatgc gatgacatac gttctaaccc accgactatt gatgttctgt
      121 accacccttg cagaaccaaa aaagccacta ccaaatattt ggtagtggct ttttttgttt
      181 tatggatttt tcgctttttc tgttatggtg ctattctttg atttcagaaa tagtatgtcg
      241 aactttagcg atagaacgac gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag
      301 agagtccaaa atgcttagtt gagaaatacg ggaagctaaa gcttcagatc ggaattctgt
      361 ttcttcagaa atagaaataa aaacgacatc gccaagttta gcaagggaag aattagcttg
      421 gctagtaata acgattgttt ttgctccagt ttttcttacg gtttcagcaa tatggattgt
      481 ttcttttgat tgccctgtgt gagaaatcaa aacagcacag tctttttccg tcaataaaga
      541 agcttccatc aattgaatat ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa
      601 aaatttatga taagcgtcag ttgctacaat ctctgaaccg ccaacaccga aaaaatagac
      661 acgtttagat tcattgataa tagtagccgc tttttttaga tccttttgtt gtaacaactt
      721 ttttgtgtca tttaaagttg aaatactaga ctcgaacacc ttttgcgcca tagtgagctc
      781 agtatctgtt ttctgaatat tttcgtggat agaaatagca gatagatcat tttcttggat
      841 caacatggcc attttaaaat ctttaaatcc attataacca agtttttttg taaattgaaa
      901 aaaagtagag tcagcaatgc ccagttcact tgctaaattg ctaataggac tatgacttac
      961 tttgcttgga ttttctaaaa tataatcggc gatagcctgt tcttttgggc tgaagtcgtt
     1021 gtacattgtt ttgatttgta gtcttatgga gacgctcatg agaattcctc attttcataa
     1081 taagattatc attttttccg ttctcattaa tgttaaagat acaaggagag taggaaaaaa
     1141 tcaaacaaaa aatatttttg taaaatctat ttacaaaaaa atttttttgt ataatgaggg
     1201 tataagaaat ggaggcgcta tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt
     1261 gaatttagct ttaaacttaa aagagcacca tcacaaagta caaggactcg acatttcaag
     1321 tacagctgtt agtcaggcaa gagaaaatga cattcctgct tatcaagaga tctctgaatt
     1381 tctagctact tttgagcaga aaaaaatcat ctggttaatg cttcctgctg gagagataac
     1441 cgaaaacatg ttaagaaaac tttttccaaa actcggacca ggagacatta ttattgaagg
     1501 cggaaactct tattataaag attcaatccg tcgagcagaa gagtttcaga aaaatgaaat
     1561 cggttacttg gattgtggga cttctggagg aatcgaagga gcacgacatc aagcctgctt
     1621 aatgattgga ggagataaag aaacttttct atccgtggaa caattattca aagatgtagc
     1681 aattgaaaga ggatatctgt atgctggtgc atccggaagc ggacacttct tgaaaatgat
     1741 ccataatgga atcgaatatg gaatgatgca agcaatcggt gaaggattcc aacttgtaca
     1801 aaaaagtgag tatgcttttg acttggaaca agtcgcacgt gtttggaatc atggttcggt
     1861 gattcgcggc tggctgatgg aaatcatgga agatcagtta aacgaacatc ccaatttaag
     1921 taattatcgg ggaatcgttt ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct
     1981 agaaatggct gttccagttc cagtaattgc aactagtttg ttcatgagga atctttcaca
     2041 ggaagaagat agtttttcag caaaagtagt ttcagcacta agaaatgggt ttggcggaca
     2101 tgaaatagtt gaaaagggtg agtaaaatgc cagattatca tatagcaatc gttaattcca
     2161 gcagtttcgg aagagtcttt ccacaccact tggaaagatt aaagtcagtt ggagacgttc
     2221 atttctttac tgttgatcaa gagattccag gaaaagaact agcggagtta cttaaaggat
     2281 tcaatatcat tattgcaagt gttacgccat tttttcgacg agattttttt gaaaacaaag
     2341 atgaactgct attgatttcc cgacatggta ttggttacaa caatattgat ttaaaagcag
     2401 caaaagaaca ccagacaatc gtatctatca tacctgcttt agttgaacga aatgctgtag
     2461 cagaaaataa tataactaat ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa
     2521 gagtcaagga cgatcgatgg gaagatcggg cggagttcgt cggtaggacg ctgtttaaca
     2581 aaacagttgg tgtgattggt gcaggaaata ccggaagcgg agtaatcgaa attttgcgta
     2641 acggttttcg ctgtgatgtt ctagcttatg atccttacaa atcagattta tacatcgaaa
     2701 gctttggtgc aaaaaaagta gaacttcaag aattactgga acaatcagat atcatttgtt
     2761 tatgtgcgaa tttgacagaa gagaactatc acatgatttc tgataaagaa gtcgatcaga
     2821 tgaaagaaaa tgtttatcta tctaatagtg caagaggcgc tctagttgat gaagaagcga
     2881 tgattaatgg tctgaaaagc ggaaagatcg caggatatgc cacagatgtt ttagaagaag
     2941 aaccgggacg tcaagatcat ccgtatttag cgtttgaaca agtagtgatt acgccacata
     3001 tatcggccta cacaagggaa tgtttagaag caatgggtga aaaatgtgtc agagatgtcg
     3061 aacagatcag tcagcacctt cttcctgaac gttctgtcca gacaaagagc aactatataa
     3121 aaggatgatg aagaaaatgg tgaacggttt aaaagtgaaa acaggccctc aattttattt
     3181 atatgaagaa ggtggtattt caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag
     3241 agtactggtc acccacggaa cggtatcatg ggaaaaggcg ctacctaaat tagtattttt
     3301 gaatgacgaa acgattcaat ttttttatca tcgttattct ggagaatgta gttatgctga
     3361 agccagacga atagctacaa tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc
     3421 ggaggaaaac tgaccgatct agtgggttat agtgccaatc ttgcaagaat cgatttcgga
     3481 gtcatcccta ctttagctag taattgtgct ccatggacac ctcttgctgt catgtataag
     3541 gagaatggac aagcagaagg gaaaacagaa cacttttaca gacaagctgc tttttttata
     3601 acagatcccc aattagtgat tgattcccct gttccatatt tcattgccgg gttagcagat
     3661 acactagcaa aatggtatga atctgagctc atcttagaac aagatttttt gcaagatgaa
     3721 tcatttttga agctagcaca agatactgcg aagatctgca aagaagagat tttgaatcat
     3781 tctgctaaag caatccaaga tatggcacaa agaaaactga catcggagtt caaacagctt
     3841 tcagagatta tctttgctgt tgctggttta gttggaggat taggagataa atacgcaaga
     3901 aatgtggcag cacatgctat gcatgacgct atcagtaaat atattccgga aagccatcgt
     3961 tttctacatg gagaaaaagt agcgtatggg attttctatc aactggcact agaaggtcgc
     4021 tgggaaaaaa tagacgagtt atatcctttg tatcgggaat tagaactgcc ggtttctcta
     4081 gaacaaatgg gattatttcc gaaagataaa cagataatag ataaaatggc tgaatttatt
     4141 gattcgaaag agaaagtgca tttgattcct atagaggtaa cacagaaatc actgatacag
     4201 tcaatgatgg aactggaagg ttatatcaga aagatctgaa ggaggaggac acatggaaac
     4261 attaagtgta gtacaaagtt tgttgattgc tttatgggta gccgcggtta tgtcccgctg
     4321 gttaggtgga ggagccacgt tgaccttacg tttttctcct ttgatgactg gattagtcgt
     4381 cgggttgatc atgggggaag ttgcacaagc gatgattgtt acagccgctt tgcaaatgat
     4441 ttatatgggg gttttttctc caggaggatc aatgccagct gaaccatcta ttgcagcagc
     4501 tatcgcagta ccagtcgcat tattagggga tctgtcgcca gaagcagcga ttgccgttgc
     4561 ggtgccagtc ggattgctcg gaagttactt gtatcagttt cgttttttca tcaatacttt
     4621 tttaggtaaa tatacagaca aagctgtaga agatttgaat acaaaagcta tggcccgatc
     4681 aattattctt tatccaacgg ttgcctcatt tttattattc gttccgcttg ttttcattgc
     4741 tttatattta ggggcgccat tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt
     4801 tatccacatt cttgaagtag taggtggagg gttagcggca atcggtatcg cgacaactgt
     4861 atacgttatc ggtcgaaaag attatctggt ctttttcttc cttgcttatt ttatgagtgt
     4921 tgttttgaaa tctttagata ttacgatggt tacttatgcc atttttggga ttattatcgc
     4981 tttgatcttt gtccagtcgc aaaaagggaa acaagtacag cctgccacaa gcaataacaa
     5041 ttcagcatct tttgatgacg aagatgacta tgatgacgga ttttaattta ggaggaatgg
     5101 gaaatggctg aaataatgga taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat
     5161 gtaacaaaag cctatctgag atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt
     5221 tacttggcgc catcactact atacggaatg atgccaatct tgaaaaaact ttataaagat
     5281 gaagactctt taaaagcagc atataaacgg cagttactgt tcttcaacac tcaattaagc
     5341 tggggtggcg gtgtgattac tggcctaatg tcttctatgg aacagcaaag agcagaagaa
     5401 gagtatgaag aaaaagagat cttgatgcag gatgacttga tgtataacac gaaagcggga
     5461 ttgatggggg cactagcagg aatcggagat gcaatcgatt caggaaccgt ccaatatatt
     5521 tttatcgcaa tcgcggtacc ttgggcacaa cagggaagtc ctttaggcgc actttttcct
     5581 tttatctgct ttgggttgta tcaggtttta ctaggggtat ttttcgcaag acaagctttc
     5641 actatgggcc gtaatgctac aggcctaaag caaaacactg gtgttcagac agcgattgaa
     5701 atgctatcag ttttagggtt gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta
     5761 gaatcttcat tgaaatttgc gatatccgga cgtgagtttg ttatccagga aattcttgat
     5821 caaatcgtgc cagggatatt acctttagct gtcgtaatgg gtgtgtactt gttctatacg
     5881 aaaaaaggat tgaaagtcac acaagcatta ttatggctga ctggaatatt aattgtgcta
     5941 gcaggaatcg gtattttata aaaagaaggg aacgtgaata agatgggtgt agtcaattta
     6001 gcgcgtgtgg atgagcgttt gatccatggg caagtaatgg taacgttatc tcaaaaaagt
     6061 ggagtgaatt ccatttttgt tgtagacgaa gttgtagcaa aagataaatt tatgagagat
     6121 ctttacaaaa atgcaggaag ccgtacagga caaaaaacaa ttgtcattac accagagaaa
     6181 gctaaattct attgggatga atatcaattt aaagaataca attgtatttt gatcgcaaaa
     6241 acagtccaaa cgatttacga cttagtaaaa cacggaatcc ccatggaaga attgaatatt
     6301 ggagggattg ctcaaaaaaa tcctgataca gatcttttag tgacgaaatc tgtttattta
     6361 aataaatcag atgctgaaaa attgaaagaa atgaatgaac aatatggcgt aaagaatatt
     6421 tattttcaag ccactccatc tgcacctaaa acaggattaa aagatgtatt gaacaagttt
     6481 gatttgtcat aaacaaatgc ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac
     6541 gagctgtttc gtgattggga atttcgttat cgctatattt accgtaagcg ccgtacaaaa
     6601 aagagtaaac agcgttttct ttcagcatta gtatcagata tttacagtat gagaacagat
     6661 gtgacagtca ttgcttacga tacgctagcc taccgatcaa aaaatattta tgttggagat
     6721 atcgaaaaag cagagaaagt tatctgtact tattacgata cacccgttca tgcattagga
     6781 agttatttca tgtttgattg gaaagaccaa aggaaaaaaa caatctactc tatattattg
     6841 tccttcattt tgttgttcag tttaggttgg tggggaatga tgatatataa caaaaatcct
     6901 catcacgtat tcgatctgct atccgtataa acaggtatca ctgtactagc atttgggagc
     6961 tactttttct tattgggaaa agcagctcgc ggatggccta gcagacaaac ttttattcga
     7021 aatacttcat ccatattaac gatgctagaa atgatccgta cgattgatga tccgagtgta
     7081 gcgtatgcgt ttgttgatga aggctgttat ggagaaaagg gcttagacag cgtgagatcg
     7141 ggtatgaaaa aagaggctat tcttttttat ctagactctg ttggggcaga tacaccgcta
     7201 cagttttcgg gaaattattt ttcaaataaa gaacagtggc tgaaacaagt tgataagttg
     7261 aaagaaaaaa acgtcaatta tatttttagt gcaagaaaaa agcaagcgca gttcttctat
     7321 ttgaccaaaa cagatttacg agggaaaaca tttaattggc aaaatgccaa tcagattatt
     7381 gctctttttc gataagtaaa aagtgcaaaa caaggagtga gaagactaat gttggaaatc
     7441 atgatcgtta cacatggtaa gttgagcgaa ggactgaaag atgcggcgga agtaattgtc
     7501 gggacatcga gccatattga aacaatgtgc ttagagtctg gggaggacat ccaaacattc
     7561 ggagagaagg tcaaagaaaa attaactatg atcaacacag cagaacccat gctagtattt
     7621 gttgatctaa ttagcgcaag tccgtacaat caagcgctct tggcagtcaa tgaactgact
     7681 gaagaaaaga aagcagccac ttatgtcata gggggcgtga atctgccaat gttgttagaa
     7741 gcaatcaatc atcagatgct tcacacaccg atagaagaag cggtggaaca aattgtcaaa
     7801 caaggaaaag ataatttaac tgtttggcat gcttcagcgg aagaaaaaaa attagatgag
     7861 gacgattttt agaaggaagg ctgaaaagat gaagtgggga tttagatggt atggtgaaaa
     7921 aggggattcg atcccattaa aatatgttcg ccaaataccg ggaataaatg ggataattgg
     7981 aactttgctg actaaaatgc ctggagatgt ttgggagatt tcagaaattg aggagttgaa
     8041 agcatctgtt gagagccaag gactggaact gcttggaatt gaaagtgtag cgatccacga
     8101 tgcaatcaag gcagggacag atgaacgaga ttactatatt gataactata tacgaacaat
     8161 caaaaacttg tctcgatgtg gggttcgatt gatttgttat agcttcaaac caatttttgg
     8221 atgggcgaaa actgatttgt ttaatgaaaa caacgatgga agctattctc ttttatacga
     8281 tcagaaaatc gtcgatcaga tggagccagg agaaatgtat caattgatac acagccagtc
     8341 aaagggattc aaattgccgg gttgggaaga agaacggttg aaaaaattcg ctcagctgga
     8401 aaaactgtat gaaggaatga ccgaagaaga attatttgaa aatctgactt attttttaga
     8461 aaaagtgatt ccagtgtgcg aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc
     8521 gccatgggaa ctttttggtt ttcctagagt gacgaaaaat cttggcgact tgaagaaaat
     8581 tctaaatgca gtagattctc catataatgg actaacgctg tgcacaggat ctttaggggc
     8641 aaatccggaa aacgatttag ttgaaatcat ccatgaggta ggttcgcgca tccattttgt
     8701 ccacttcaga aacgtggagt ttttaggaga aagaaagttc aaagaaacgg cacatccctc
     8761 tacagaaggc tcccttgata tgtatcatat tatgaaagcg ttggtgcaag aagggttcga
     8821 cggtcctata cgccctgatc atggtagaac cgtgtggggt gaagtggcta tgcctggata
     8881 tggcctgtat gatcgtgcaa tgggactcac ttacatgcaa ggtctatatg aggcgataag
     8941 taaggaaaaa ccactgggag agcattatga ataaaatcaa gctcttcatc tttgatatgg
     9001 atggcctttt attagaaact ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa
     9061 agtatgatta tgaaatgcgt aaggaagtct attatttact aactggccaa acagaaatgg
     9121 cgataagaga acaaatgggc attttgtacg gagaagatgt tccatatatc aaatggcgag
     9181 aagcaatcaa tcaatataaa gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa
     9241 aaggagcaga agaaatttta tcttttgcaa aagaaagagg aatccataca atcgtggctt
     9301 cgtctaatac acgggtaaaa gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt
     9361 ttgatcaaat catttcaggg gatgatgtaa aaaaaggaaa gccggaacca gagatctttt
     9421 tgaaagcttg ttcaaaaatg aatattcctc caagtgaagc acttgtattt gaagactcga
     9481 ttgcagggat cgaagcagca aggcgagcgg gcattttgtc ttttctcatc aaagatcatt
     9541 tgatagggtt gcccgatcat aatgggaagc ataagttgaa agtagatgta agtttatatg
     9601 aatccaataa agcagactat gaattttatg acctgcatca agcaaaacga tttttagaaa
     9661 gtaaagggtt gtatctctaa taaagaggac tattttctag tcctctttat ttattgtcct
     9721 gcacgactga gggaacagat gaaaaagaag taatttttta ttttgaagta tctaccttat
     9781 ttgtagtgaa caatttagaa caaacacaaa acgcatctac tgttatctac cagtagatgc
     9841 gtttaaagag aagaacgaga atcgtccata tgagggtttc cactccttat gcagattttt
     9901 ttaattcttg cactgcttgt tcgatcgtaa cacctgtcgc tacatgattg gtattttctt
     9961 tgtcaaaaac gacaaataga ttcaaatctg tatccaaggt tactcgatat tgtatatctt
    10021 tgttatagaa tgtcatagcc attctccttt ctttaaatac ttgctgaaag gcaacgaacg
    10081 cgttgataac gaaattccat ttataagtat accaccaatt gaaaggtttt catagttaag
    10141 aaattttaaa gtcaaaagtt gcagataaaa gcaaataaac aagaaaacaa aaagtgaatc
    10201 aatcgcggtt attcaacttt ttcttcgatt cgaagacaat agaagacaat gaaatttata
    10261 tttcattgca ttatccttgt gttattagaa aaagcatgat atattaaatg agtaatctat
    10321 tttgaaacgt aatttgagcg atatatctac atagataaaa ctccttttgc ctatcaaata
    10381 tttattgcaa cgaaaaaacg tacattgata cgtatcagga ggaatttatt tatgaataac
    10441 ggtacagtaa aatggttcaa cgcagacaaa ggttttggct ttatcactgg tgaagatgga
    10501 aatgatgtat ttgcacattt ttcagctatc caaggtgacg gcttcaaaac tttagaagaa
    10561 ggccaagcag tgtcttacga tgttgaagat ggtcaacgtg gccctcaagc aacaaacatt
    10621 gttaaagctt aataaaaatt gacttttaac cgtcttttcg aaagaaaa
//
LOCUS       DAYYBH010000089        10529 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000089 DAYYBH010000000
VERSION     DAYYBH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.092"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..1659)
                     /locus_tag="ACHAUG_002472"
     CDS             complement(100..1659)
                     /locus_tag="ACHAUG_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8481531.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1814..4048
                     /gene="pcrA"
                     /locus_tag="ACHAUG_002473"
     CDS             1814..4048
                     /gene="pcrA"
                     /locus_tag="ACHAUG_002473"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8481532.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4065..6101
                     /gene="ligA"
                     /locus_tag="ACHAUG_002474"
     CDS             4065..6101
                     /gene="ligA"
                     /locus_tag="ACHAUG_002474"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8481533.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6207..6512
                     /gene="gatC"
                     /locus_tag="ACHAUG_002475"
     CDS             6207..6512
                     /gene="gatC"
                     /locus_tag="ACHAUG_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8481534.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6512..7984
                     /gene="gatA"
                     /locus_tag="ACHAUG_002476"
     CDS             6512..7984
                     /gene="gatA"
                     /locus_tag="ACHAUG_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8481535.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7981..9411
                     /gene="gatB"
                     /locus_tag="ACHAUG_002477"
     CDS             7981..9411
                     /gene="gatB"
                     /locus_tag="ACHAUG_002477"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8481536.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9421..10476
                     /locus_tag="ACHAUG_002478"
     CDS             9421..10476
                     /locus_tag="ACHAUG_002478"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8481537.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
BASE COUNT         3590 a         1973 c         2309 g         2657 t
ORIGIN      
        1 aagaggctgg gttttcccag cctcttcttt ttttatagaa tcatatagaa tcaagtagtg
       61 tagatgaata gttgttgtaa aatagttttt ccatgttcgt taaaatgatt gcttcaaatt
      121 tgtgatagaa gatttagcct cgatttcctg cttgattctt ggcaagtttg cttcatcagt
      181 caagataacc aaaatatctc ctacattcat gatcgtatca ccgtgagtca agatttcctg
      241 atttcctctg cgtatagaga ttagcagcat atttttaggc caattgaagt ctcgtaccat
      301 taatccgtct aaagaactct ctgccgttac tggaaaatca aaagttgtcc ttgcaccttt
      361 tagtgaggcg gtctgagtat ttccaagtag acgttccaat aatgattcgt aaattggatt
      421 accgccgaga aggtcattga cgatataagc aacaagagca gccaatccta aaggcatcag
      481 atgattaaga gagccaacca tttctgtcac taaaataatt gctgtcagcg gtgcttttcc
      541 aatcgctgtg aaataaccag ccatggcaat aacgacaaaa tccttgatgt agattggatc
      601 catcccgaaa tcaaccataa tgtttccata aaagctaccg attaaagccc ccaatgtcaa
      661 tatcggaagg aaaatccccc ctggaagacc cccaccgtaa gaaatcatgg aaaaaacgaa
      721 tcgaagaaca aacaaaccga taatcataat gattggcaag gaagcatgtc caaaagctaa
      781 aacgatttga ttgcctccgc ctagagtact aggccataaa taaccaattg gcaaaatcag
      841 caagaaagga acgaacccgt gaaaataagc tggtaaaggc aatcggttgt aaaattttgg
      901 caaagccagc aagacattct gataaataaa acctaagatg cctagaagta ctcctaagat
      961 cactaaggaa ccataatatt tcaacggcaa agaaggaagg tcacctagat ataaaacagg
     1021 tctcaagccg aaaaagtaaa gagagacgaa gttagcagtg attgctgaag caaatgatgt
     1081 cagccaaaca agtggagaaa aggagtgatg gatttcttct aaaacaaaga gtaatcctgc
     1141 gataggagca ttgaatgctg ctgcaagtcc gctacttgca ccgctagaaa ttaaaatctt
     1201 ttcttctgaa gatggactcc gaaacaattg tccagctcct tgtccaatgg atgcgccaag
     1261 ctgtatagaa ggtccttctc tacctagaaa aaggcccgca ccaactgaaa gtacaccgcc
     1321 aatgaacttt ttccataaaa cagagaacca gttcattgat aattccccgc gaagctgtcc
     1381 cactacttgt ggaatcccgc ttcccttgat atccggttca tttttgacca gataacccac
     1441 gaaaaatgct gcaaggatgc agcaaatagc taggccaatt aagtaaatcg gctgttcccg
     1501 actcatttca tagatacttc gaactaaatc gcttagcttt tcgatagata atcgaaacag
     1561 gctaacgacg attcctgtca gcagtccaac aaatattcct tttagaataa aatatatctg
     1621 agtcttatct aatttcttga tttttccttt tttttccatt caaacgcctc gctttctttt
     1681 aatttagcgc gaaatcaacg aattgacaag gaaaaatcca taaaaagaaa caaatgattt
     1741 tgaactagct agtttaatga aagccaagaa aatgctataa ttaacaagat tgataaaaga
     1801 aaggatgttg ttaatgtcat ccaaagctga attattaaac gggatgaacc cccgtcagaa
     1861 agaagctgtt cttcacactg acggaccatt actgctaatg gcaggtgcag gaagtggaaa
     1921 gacgagagtg ctgacacacc gcattgccta tttgattgag gaaaaagaag tcaatccttg
     1981 gaacatccta gcgattactt ttaccaataa ggctgcaaaa gaaatgaaag aacgtgtcaa
     2041 tgcgattctc gcatctggag gcgaagatgt ctgggtatcg acttttcact cgatgtgtgt
     2101 gcgtatttta agaagagatg ttgattttat cgggtataac cggaacttta caatcatcga
     2161 ttcttctgaa cagctgacct tgatgaaacg gatactaaaa gaactgaaca ttgatccgaa
     2221 aaaatatgat cctcgaagta tattaggaac aatctctcaa gcaaaaaaca gcctgcagac
     2281 ccctcaggat tttgcaaaaa tgcaaggcag ttattacgaa gagattgcgg caaaatgtta
     2341 tgctgcttac caaaaagagc ttcaatataa tcaatgtatg gactttgacg acctgatcat
     2401 gaatacgatt cgattatttg aagaacatcc tgattcactc acgtattatc aaaacaaatt
     2461 ccattatatt catgtagatg agtaccaaga tacaaatcac gcacagtata cgctagtcaa
     2521 tttattggca ggtagattta ggaacctgtg cgtcgtggga gatgcagatc aaagtattta
     2581 cggctggcgc ggtgcagata tgcagaacat cttggatttt gaaaaagatt atccagatgc
     2641 tgcagtaatt ttgctagaac aaaactatcg ctccacgaaa aacatcctta gcgcagcaaa
     2701 ccaagtcatt gaaaacaata gcaatcgaaa accaaaaaac ttgtggacag aaaacaaaga
     2761 aggaaacaag atcacttact accgtgcaga taatgaacga gacgaaaccc gttttatcgt
     2821 agaccggatg caagaagaga ttcgaagcaa tcatcggaat tacggcgact ttgccatctt
     2881 gtaccggacg aacgcacaat ctcgtgtaat ggaagaaact ctgttgaaag caaatattcc
     2941 ttataaaatg gttggagggc acaagttcta cgatcgtaaa gaaatcaaag atattttagc
     3001 gtatttgaat gtgctggtaa atcctcaaga ttcaatcagt tttgagcgta tcgttaattc
     3061 gcccaaacga ggcatcggac caggatctat cgaaaaacta cgcagttttg cttctcttca
     3121 tgaatggcca ctgcttgaag cagcgcaaaa tgtggatctg gcaaatatcg gtggaaaagc
     3181 aggacagcaa ttaggtgcat ttggagagat gatccaagaa gtcactcaga tgatcccata
     3241 cttgacagtg acagaattga cgaaagaggt tttggatcgt agcggatatc tagaagactt
     3301 aaaaatccaa aatacattag aagcacaagc acgcatcgaa aacttggagg aatttttaac
     3361 ggtcacacaa gaattcgaca aacaattcga acagcaaaat gaagaagacg ctgatgcacc
     3421 agaagaaaaa ctaacggtct tcttaaatga tctggcactt gtttccgaca ttgacaattt
     3481 agaagaagat gcttcccaag tgacattgat gactttgcat gcagccaaag gattagaatt
     3541 tccagttgta ttcctgattg gtctagaaga gggggttttt cctttatctc gagcattgat
     3601 ggaagaaagc gaactggagg aagaacgccg tctcgcatat gtggggatca cgcgagcaga
     3661 agaagcgttg tatttaacaa atgctttctc cagaacatta tatggacgga cgcaatataa
     3721 ccgaccttct cgttttgtag aagaaatcga tcaggagtta ctggaaatcg aaggcatgcg
     3781 tcctacgcct aaaaaaacgc ctgtattcgc taaaaaaaca gcatatagct ataaacagcc
     3841 agagacagcg gttgttccct ctaaaagtgc tactggaggg gaaaataaca gctggaaacc
     3901 tggagataaa gtcaaacaca aaaaatgggg tctaggaaca gtcgtccgag taagcgggac
     3961 gtcgaaggat ctagaactag atgtggcttt cccaagtcaa ggagtaaaac gtcttttggc
     4021 tgcctttgcg ccaattgaaa aagcttagta gtggagggta aatgatggaa catgaaatga
     4081 cgattgacga agctgctaaa cgagcagaag aacttcgtac aagactgaac caatggtctc
     4141 gcgaatacta cgtagaagat aaaccaacag tggaagacta tgtttatgat aaagaatacg
     4201 cagaattagt agcaatcgag gaacaatatc ctgatttgat cacgtctgat tctcccactc
     4261 aacgagtagg cggaaaagta ttagaaggtt ttgaaaaagt cactcatgat attccgcttt
     4321 acagtttgaa cgacgttttc agcaaagaag agctgatagc ttttgatcaa cgtgtacaaa
     4381 aagcagtggg acgtgtagtt gactattgct gtgaactaaa aatagatgga ctttccgttt
     4441 ctttgcgtta tgaagatgga aatttcgtga gaggtgcgac tcgcggggat ggaactgtag
     4501 gagaaaacat cactgaaaac ctaaaaactg tccggtcggt cccaatcaaa ttaaaagaac
     4561 caatgaacat cgaagtaaga ggcgaatgtt tcatgccaaa acggtcattt gtccaattga
     4621 accaagacag agaagcagag ggaaaagata tctttgcgaa tcctcggaac gccgcagctg
     4681 gttctttgcg tcagttagat tctaaaatca cagcgaaaag aaatctggac acttttttat
     4741 acacagtagc tgatttcggt cctatgcaag caaaaaccca gtatgatgcg ctagaagagc
     4801 tagaaaaaat tggattccac acgaatcggg aaaaacgtct atgtcattcg attgatgaag
     4861 tgtggtcata cattgaagaa tatcatgaca aacgtgtgga tttgccctat gaaatcgatg
     4921 gaatcgtcat caaagtgaat gaattctcgc tacaagatca acttggtttt actatcaaag
     4981 caccgagatg ggcagcagct tacaagtttc cgccagaaga agttgaaacg ctaatcgaaa
     5041 acatcgaatg gacagtcggt cgaacagggg tagtaacgcc aacagctatc atgacaccag
     5101 tccgagtagc aggaacgaca gtaagccgtg ccagcctgca taacggtgat tatattaagt
     5161 tgaaggatat ccgcttgaaa gatacagttc tgatttataa agcgggagac atcatcccag
     5221 aagtttcgca agtcgtgtta gataaacgac ctaaagacag cgaagaatac caattgccta
     5281 cacattgtcc agtttgtgga agtgaacttg tccatttgga tgaagaagta gccttacggt
     5341 gcatcaatcc taaatgccct gctcaaatga aagaaggttt gaaccacttt gtttcaagaa
     5401 atgcgatgaa tatcgatggg ttggggccgc gcgtactaga acagatgtat gataaaaaac
     5461 tagtagcaga tgttgctgat ctttacaaat tgacagaaga agagttattg acgttggaca
     5521 aaataaaaga aaaatcagca aataatattt tgacagcaat cgacaacagc aaagataatt
     5581 cggtcgagcg gttgattttc gggttaggaa tccgacatgt cggagcgaaa gcagcaaaaa
     5641 ttttagcaga acatttcggt gatctagaaa cattgagtaa aagtgactat gagtcgatta
     5701 ttgcgcttga tacgatcggt gatatcattg ccgacagtgt agtcacttat ttcagtaacg
     5761 aagaagtcca tgaattgatg aatgaattaa aacaagcagg cgtcaatttc gaatacaaag
     5821 ggcttcgaaa tgcccaattg caagaagtcg agtctccttt taaagaaaaa acagtagtcc
     5881 ttactggtaa actaacacga tttactcgtg aagaagcaaa agagacgatc gaaaatcttg
     5941 gcgggaaagt gactggtagc gtatcgaaaa aaacggatat cgtggtagca ggagaagatg
     6001 ctggcagcaa gctgaccaaa gcacaagaat taggaatcga agtttggaca gaagatcaaa
     6061 tggcagatgc attggctaaa agtcgctcag tagaagaatg aaaaatgagt tttttcagac
     6121 aaatttcaat aaacaatttc agacacggca ctcttgtgtt aaaatagaga gcaaagtttt
     6181 taactcgttt agaaagaagg ggtagtatgg cgattagtga agaacaagtg aaacacgttg
     6241 ccaaattagc aaaacttgcg ttcgcagatg aagaacttgc aagttttacc gaccaattag
     6301 gtaaaatcat cgatatggtt gaaatgctgg aagaagtaga tacggaaggt gttccattta
     6361 cgtcgaatgt ggttgaaaca gtaaacgtga tgcgacaaga tcgggcgcaa gctggatgga
     6421 accgcgatga attattgaaa aatgtgccgg aaacagaaga cggctttatc aaagtacccg
     6481 caatcatcga taatggggag gctggcgcat aatgacagaa ttacacaatc gttctctaga
     6541 agaactgcat catatgcttg tatcgaaaga aatcactgta caagatttga caaaaacaac
     6601 atttgaacga atcaaagaaa cagagcctga ggtcgaggca ttcattacat tgaacgaaga
     6661 aaaagcattg gaacaggcaa aagctttaga tgaaaaaggg attgatgaaa gcaacgtcct
     6721 tgctggtatt ccaattggta tcaaggataa tatcgtaaca aaagatatct tgacaaccgc
     6781 tgcatctaag atcctttaca attttgaacc aatttatgat gcaacggtca tggataaaat
     6841 ctatcaagca gatatgatcc ctgttggtaa gctaaacatg gatgaatttg ctatgggtgg
     6901 aagtacagaa acttcttatt tcaagaaaac aaaaaatgct tgggacccaa caaaagtacc
     6961 tggtggctct tctggtggtt ctgcagcagc agttgcagct ggacaaattc cagtttcttt
     7021 aggtagtgat actgggggaa gtatccgcca accagcagct tttaacggca tcgtagggat
     7081 gaaacctact tatggccgtg tctcacgttt tggtttgatt gcctttgctt catctcttga
     7141 ccaaatcgga ccaatgaccc gtacggtgaa agataatgca ttagcattga cagctatcag
     7201 cggttatgac gaaaaagacg gtacttcttc aggcgtttca gttcctgatt ttgcagaggg
     7261 actgacaggc gacatcaaag gaatgaagat tgctttacct aaagaatact taggcgaagg
     7321 tgtggcccct ggcgtaaaag aagctgtatt gaaagcagca gaaacattcc gttcattagg
     7381 tgctgttgtg gaagaagtca gcttgcctca ttcaaaatac ggtgtggctg tttattatat
     7441 cattgcctca tcagaagcaa gttccaattt gcaacgattc gatggtatcc gctacggtta
     7501 tcgctctgaa aatgtgaaat cattagatga tgtatatgtc aactcacgtt cagaaggatt
     7561 cggtgaagaa gtaaaacgcc gtatcatgtt aggtacattc tccttaagcg cgggttatta
     7621 tgacgcttac ttcaaaaaag caggacaagt ccgtacattg atcaaacgag attttgaaaa
     7681 agtctttgca gattatgact tgatcatcgg gccatcttca ccaacagttg cattcggaat
     7741 cggtgagaac attaacgatc caatcacaat gtacatgagt gacatcttaa cgatccctgt
     7801 caacttggct ggacttccag gtatgtctgt accggcaggt ttatcagaag gattaccagt
     7861 tggattgcag atcatcggta attattttga tgaaaaaacg atgtatcaag cggcatatgc
     7921 gtttgaacaa gcaacagaat tccataaaca acaaccagct attttaggag ggaaagacga
     7981 atgaattttg aaacagtcat tggactagaa gtccacgtcg agttaaaaac caactcaaaa
     8041 attttctctc cagcgccagc tcattttggt gcagaaccaa acagcaatac aaacgtgatc
     8101 gactggggtt atccaggcgt gctgccagta atgaataaag ctgcgctgga attcgggatg
     8161 aaagcagctt tggcattaaa ctgtgaaatc tctaaagata ctcattttga ccggaaaaat
     8221 tacttttacc cagataatcc aaaagcatat caaatttccc aatttgatca accaatcggt
     8281 catgacggat ggatcgaaat cgaagtagaa ggtaaaaaga aaaaaatccg gatcgaacgt
     8341 gtccatttag aagaagatgc tggaaaaaac atgcatggta ttggcggtta ttcttacgtt
     8401 gatttgaacc gacaaggcac gcctttgatc gaaatcgttt ctgaagctga tatgcgttca
     8461 ccagaagaag catacgctta tctagaagca ttgcgatcaa ttatccaatt tacagaagtc
     8521 agcgatgtga aaatggaaga aggctctatg cgctgtgatg ccaatatttc tcttcgccct
     8581 tatggacaag aagagttcgg tacgaaagca gaattgaaaa acttgaactc tttatctttc
     8641 gtgaaaaaag gtttagcttt tgaagaaaaa cgtcaagcaa aagttcttct ttcaggagga
     8701 gaaatccaac aagaaacacg ccgttttgac gaaacaacga ataaaacagt tttgatgcgt
     8761 gtcaaagaag ggtctagtga ttatcgttac ttcccagagc ctgatatccc gaaattcgtg
     8821 atcgatgacg aatggattca aaaagtaaaa gctagtttgc ctgagatgcc agcttctcgt
     8881 cgcgaacgct atatccgtga gtttggactt ccagagtatg acgcaatggt attgacactt
     8941 acaaaagaaa tgtctgattt ttttgaagct gttttggaaa atggtgcaga tgccaagcaa
     9001 gcatctaact ggttgatggg tgaagtttct gcttacttga acagtgagaa actggaatta
     9061 gctgagacaa aattgacacc tgagaactta gcaggaatga ttcgtttgat cgccgatgga
     9121 acgatcagct caaaaatcgc caaaaaagtc ttccgtgaat tgatcacaaa tggcggagaa
     9181 gcccaagaag tagttgaaaa aaatggttgg gtgcaattat ctgatccgaa caaactactg
     9241 ccaatcatta atgaaatttt ggataacaac caacaatctg tcgatgattt caaaaatgga
     9301 aaagatcgtg cggttggctt cctagtcggt caaatcatga aagcaactaa aggacaagca
     9361 aatcctggtg tcgtgaataa gctacttaaa gatgaattag cgaaacgata gaggctgaaa
     9421 atgaaaaaag cacgcgtaat ttataacccc acctcgggaa aagaattatt gaaaaaaaat
     9481 ttggcggata tcctacaggc attagaagaa gcaggatttg aagccagcgc ctatgcaact
     9541 acaccagaac cagattcagc caagaatgaa gcatgtagag tcgcagaact aggttttgat
     9601 ctagtagtgg cagctggtgg cgatggcaca atcaacgaag tggttaatgg aatcgctccg
     9661 cttgaacacc gtcctaaaat ggccatcatc ccagctggta caacgaacga ctatgctcga
     9721 gcattaaaaa ttcctagaga taatattaaa gcagcagcag aagtgataaa aaaacaccaa
     9781 accgtaaaaa tggacatcgg cctttctgat aacagttact tcatcaatat cgctgcaggt
     9841 ggctatttga cagaattgac ttatgaagtt ccatcagaac taaagagtat ttttggttat
     9901 cttgcttatc ttgcgaaagg agcagagatg ctccctcgag taaagccaat caaaatgcgc
     9961 ttgaagtatg atgaagggga atatatcgga aatgcttcga tgtttttcct agggctgacg
    10021 aactctgttg gaggattcga acagatcgcg ccagatgcca aattggatga tgggaagttt
    10081 tctttaatca ttgtgaagac agcaaatatc tttgagatct tgcatattgc agcactcatg
    10141 ctgaatggcg gtcgacatgt cgatgatcca agaataatct ataccaaaac aagcagtttg
    10201 ttcgttgaaa cagaagatga tatcaatcga ccagtaatga tcaacttaga tggtgaatac
    10261 ggcggggaag caccgatgca tttccaaaat cttcatcaac acattgagtt ctttgccaat
    10321 accgatcaga ttcctgatga agcaatcaca ggtacagatg aagaagaatt agaggttgtc
    10381 agcaaagaat tcgtaaaaga agtcgaacgt ttaacggatg aagatatcga cggtgacgga
    10441 aaaatagcag acgaaacacc taatcaaaag aattaatcaa aagccggtgc tgaacgtagt
    10501 aaggtcagtg ccggttttag ttgtgtaat
//
LOCUS       DAYYBH010000090        10517 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000090 DAYYBH010000000
VERSION     DAYYBH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.093"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACHAUG_002479"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACHAUG_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACHAUG_002480"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACHAUG_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACHAUG_002481"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACHAUG_002481"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8481538.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACHAUG_002482"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACHAUG_002482"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8481539.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACHAUG_002483"
     CDS             5704..6489
                     /locus_tag="ACHAUG_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8481540.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACHAUG_002484"
     CDS             6502..7353
                     /locus_tag="ACHAUG_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8481541.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACHAUG_002485"
     CDS             complement(7477..7929)
                     /locus_tag="ACHAUG_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481542.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACHAUG_002486"
     CDS             complement(8394..8762)
                     /locus_tag="ACHAUG_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8481543.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="ACHAUG_002487"
     CDS             8912..9394
                     /locus_tag="ACHAUG_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8481544.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="ACHAUG_002488"
     CDS             9431..10441
                     /locus_tag="ACHAUG_002488"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8481545.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3576 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaa
//
LOCUS       DAYYBH010000091        10390 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000091 DAYYBH010000000
VERSION     DAYYBH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.094"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(103..345)
                     /gene="acpP"
                     /locus_tag="ACHAUG_002489"
     CDS             complement(103..345)
                     /gene="acpP"
                     /locus_tag="ACHAUG_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8481546.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(467..1468)
                     /gene="plsX"
                     /locus_tag="ACHAUG_002490"
     CDS             complement(467..1468)
                     /gene="plsX"
                     /locus_tag="ACHAUG_002490"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8481547.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1517..3553)
                     /gene="recG"
                     /locus_tag="ACHAUG_002491"
     CDS             complement(1517..3553)
                     /gene="recG"
                     /locus_tag="ACHAUG_002491"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8481548.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3670..5352)
                     /locus_tag="ACHAUG_002492"
     CDS             complement(3670..5352)
                     /locus_tag="ACHAUG_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8481549.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5404..5766)
                     /locus_tag="ACHAUG_002493"
     CDS             complement(5404..5766)
                     /locus_tag="ACHAUG_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8481550.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6036..6224
                     /gene="rpmB"
                     /locus_tag="ACHAUG_002494"
     CDS             6036..6224
                     /gene="rpmB"
                     /locus_tag="ACHAUG_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8481551.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6462..9131
                     /locus_tag="ACHAUG_002495"
     CDS             6462..9131
                     /locus_tag="ACHAUG_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8481552.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9586..10332)
                     /gene="truA"
                     /locus_tag="ACHAUG_002496"
     CDS             complement(9586..10332)
                     /gene="truA"
                     /locus_tag="ACHAUG_002496"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8481553.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2838 a         2237 c         1866 g         3449 t
ORIGIN      
        1 ccagaattat cagaaaaatg acttttacgt gtcaaaaaga ttaaaaaaag aaggataaaa
       61 agctgcatgc agctttttat ccttctttgc ttttatttga aattatttaa gatggctgct
      121 gatataatct actgcgccac cgactgtttc aatttgttca gcgtcttcat ctgaaatttc
      181 cgtaccgaat tcatcttcaa gctctaaaac gaattccata atactaattg aatcagcttt
      241 taaatcatct ttgatgttca attcatccgt cactttatcg gtatctatct caaaatgatt
      301 tgaaataatt ttcgctactt tgttaaatac ttcttcgcga gtcaactttt tcacctccaa
      361 gaatcataca cttgtcaatt acctacacca tggctaattt tactggcatt tttcaggaat
      421 gtccaccttt ttttcaatgg aacggatcgc ttcagatatc caaatactat tcttcgcttt
      481 tttcaaattg ttcgactaat tgtccaacta catctgtctc aagcattgtg tggatttggc
      541 ggatcgtata gcgaacagca tcggggcctg ttgctccatg agttttaatg actggtgctt
      601 tcaagccaaa caaaacggca ccgccatgtt tggaataatc catttctgct ttcaaagaac
      661 gtaagccgtc ttttaaaagc atagcgccca tttttccttt gatcccttca tttagaatag
      721 cagattttaa tagattcatc atattcattg ctgttccttc aatagatttc aaaacagcgt
      781 tgcctgtaaa tccatctgtg accactacat cagccacacc attgagtagc tcacgagctt
      841 ctacgtttcc aataaaattc aagcttgttt cattctgaag gagttcatag gcttttttcg
      901 tcacttcact accttttgtt gcttctgttc cgttgtttaa gagtgcgaca cgaggacgtc
      961 cgatacctcg gactttacgt gcataaaaag aacctaaaat accgtattgc aacaaatgtt
     1021 ctggtttgtt ttctgcgtta gcgccaagat ccagcatatc gaaaccggca ccttctttcc
     1081 ctactactgg caaggtagac atcaagccag ggcgttcgat atttttgata cgtcctacga
     1141 tgaacaaccc tgctgctagt agcgcgcctg tattccctgc tgaaaataca gcatctgctt
     1201 caccgttttt cactgcttgt gcagcaagga ccattgatgc agtctttttt cgtcgaattg
     1261 cttttactgg ctcatcatca ctattgattt tttcgtctgt atggataatc gtgatgtttt
     1321 tttcatttgt taagtatttc ttgatttctg cttcttttcc gtaaagtaag aattctactt
     1381 ctggaaaatc ttgctgcgct aaagccacgc cttctacaat tgcttgaggt gcgtgatctc
     1441 cgcccattgc atctacggca attttcattg tttccactcc tttttccgtc atcagtataa
     1501 agtatagcac agtctgctaa tcgaaataac gattatcctg attttctatt tggaggcttt
     1561 gcgctaaagg ctgatactca ggaagcatct gccaatcctt tatttgccaa agggctgcag
     1621 cctcttctct ggctacctcc aaaacatttc catccgtcac caagtcagca atcgcaaatt
     1681 gaggcagacc agattgacgg aagccaaata cttcaccagg accacgtaat tccagatctt
     1741 tttcgcttag tacaaaacca ttggtggtct ctgtcatgat tttcatccgt tccacaccta
     1801 gttcattttt aggattagca actaagatac agtaggaagc atcacttccg cgtccaacac
     1861 gtccacgaag ttggtgcagc tgtgccaatc caaatcgatc cgcatccatg atcagcatga
     1921 cggtcgcgtt cggcacgttt acccctactt caattacagt agtcgaaact aatatctgca
     1981 tctggttgtc tttgaaggct tccatgatta cttctttttc ctgattcttc attttaccat
     2041 gtaacaagcc aacttcatat tttggtgcaa atagttcacg aagtttttca tagatttcca
     2101 ctgcattttt tacatctaat gcttctgatt cttcaatcag cggacagatg acatacattt
     2161 gatgtcctct tgccagttct tttttcgtcc aatccagtac actgtccaat tgaggtgttc
     2221 ttacccagcg tgtttcgatc gggatacgtc cagctggaag ttcatcaatg atagatacat
     2281 ccatctcacc atatgctgta atagctaatg ttctaggaat cggagtcgct gtcataaata
     2341 agacatctgg tcgccagcct ttttcacgaa gtactttccg ctggttcacg ccaaaacggt
     2401 gctgctcatc cgtaataact agacctaaat tctgaaattc gactccatct tggatcaatg
     2461 catgtgtgcc gatcacgacg ttaatttccc cttgctctag tttttcaagg agctcacgtc
     2521 gttcttttgc tttcgttgac cccgtaatca atgcgatggt tacttcattg ggatcaaaca
     2581 gctgttgcaa gctttccata tgttgttgtg ccaagatttc tgtaggcacc atcaacgcac
     2641 cttggaaacc tgctgtaacg gtagcgtata acgcaatcgc ggcaaccact gtttttccgc
     2701 tacctacatc tccctgcaaa agtcgctgca tatgctgcgg actgcgaaga tcccgacaga
     2761 tttcattcgt tacacgtttt tgtgcatttg tcagttcaaa aggtagcttt tgagtaaatt
     2821 gtttcaattt ttggacatca taaaggatct ccaaaccatt cgtttctgct ttttctgctt
     2881 ttttcaatcc ttgaagacgc atttgaaata aaaagaactc ttcaaaaacg acacgtcgtt
     2941 tggcttgatg gttttcttcg tggtcttttg gaaaatgcat acttcttaca gcagaagctc
     3001 ggtcaagcag tcgatatttc tccacaaggt tgtttggcag attttcttca atcaatgtgc
     3061 cgtattcttg aaaccctttt ttgattagat caactaatgt cgtttgacga attgatttgt
     3121 tgacgtgata tatcggcgaa aagtcatcca ttgtttgaga acctaaaatc ttcatcccgt
     3181 tcaaggcttt acgctttgca tcccatttac cgtatacggc gatttcctcc gataaaatga
     3241 ctttttcttt tagataaggt tgattaaaaa aagaaacgtt gaacacatca tgttcttgca
     3301 tcatacgaaa ttgcaatcgg ctttttttat aaccaaatcg attcatcaca ggaggcgaga
     3361 ctacgattcc tttcaaagta accttttctt gatcctggat ctcgtttaga tttctttcct
     3421 gaatatcttc gtagcgaaaa ggataataag tcagtaagtc ttcaatcgtt tggatgccca
     3481 agctggcaag actagctgct cttttttctc cgactcctgc cagcatagtg attggatcat
     3541 ctaaagaacg cataaatttt tcctccctcc tttaacaaaa aaaggacttg gtcagcgatc
     3601 atacctgatc actaaccgaa ggccttttat gaatgaatac tgtgttagaa agatattctg
     3661 acacagaaat tattcagcag aaaatagata aggatagaca ggctgatctc cttggtggat
     3721 ctcgatttcc aaatcaggat gtttttctgt tagtttctca gcaaattcat ttgcttgttc
     3781 aactgttcct tcatcacctg ccaagatcgt tacgatctct gtgtcctcat tgatcatttg
     3841 ttctagtgta cttaacgctg ttgctgtgat atctgggtta gagatcagga tttttccatc
     3901 gatcatgccc aaaaagtctc cttctttgat ctctacaccg tcaattgagg tatctctgat
     3961 cgcgtgagtg acttgaccgc tcacaacgct agacaacatt tctgtcatcg tttctttgtt
     4021 ttcttctaaa gaagcttgat cattgaatga aagcatagct gtcaatcctt gggaaatcgt
     4081 tcttgaagga acaactgcta ctgggatatc tgctacttct gctgcttgat cagctgccat
     4141 gaagatgttc ttattgtttg gcaagacaat cactttatct gcgtttactt cttgaatcgc
     4201 tttaacaatg tcttccgtac ttggattcat tgtttgtcca ccactgataa cataattagc
     4261 gcctaaactt tcaaacagct cttggacgcc tttaccagca gcgatcgcta cgataccata
     4321 cgggatacgt ggtatttctt ttttcgcaaa ctcgttcact tcttcatcgt gttccaaaat
     4381 cgtctcatgc tgcaagcgca tgttgtcgac tttgattttg actaatgaac cgaatttttg
     4441 tccataattc aagacttctc ctgggttttc tgtatgcacg tggactttga tgatctcgtc
     4501 atcattgacc acaagtaaag aatctcctat accatctaaa tagttacgga attcttcgta
     4561 atcgaatgta ctatcgactg taggtccttc tccgattttt accatgattt ctgtacagta
     4621 accaaaatgg atatcttctg tcgccatttg accttggaca ctgcgatgat gttctgcatt
     4681 gaccatatca tccatttctg ctggagaagg ttcatatgtt tcatccgctt ggaattctcc
     4741 attcaatgca cttaaaaagc cctcataaac aaacagcagt ccttgccctc cactgtctac
     4801 gacaccgact tcttttagga ctggaagcaa gtctggagtt ttagctaaag cacgctttgc
     4861 tccttttaca acagctgtca ttacttcaac gacatcatca gtactttttg ctttcttttc
     4921 tccagctttc gctgcttctc tagctactgt caaaatcgtt ccttctactg gtttcattac
     4981 tgctttatac gcagtttcaa ctccatgagt gaaggctgca gcaagatcag aagcatttaa
     5041 tgagacaaca tctggaattt gcttagaaaa tccacgaaac aattgagaca aaataacgcc
     5101 ggaatttccg cgcgcaccca tcagcaatcc ttttgaaagc attgctgcta attctcctac
     5161 tttttctgag gttgaatcta ctactgcctt agctccacta gtcatagata gattcatatt
     5221 tgttcccgta tcgccatctg gcacagggaa tacatttaat gaattgacgt attctgcatt
     5281 tacttgcaga cgattcgcac ctgcctggac catttcctgg aactggcttg cacttatttc
     5341 tgttacactc acctagataa tcctccttca gatcgactgc tgagttacca gccagtctcc
     5401 tgcctagtct ggcagtacac gaacaccttg tacaaagaca ttaactgaat tggcagttac
     5461 gcctaataat gcctctagat tgtatttcac tttttcttgg acgttgcggg aaacttcaga
     5521 gattttcgta ccatagctga taatcgtata aacatctaca gcaataccat tatcttcttg
     5581 gcggacaact actcctcttg agtaattttc tttacgcaaa atctcagtaa tattatcttt
     5641 aatttgattt ttgctggcca ttcctacaac accgaacaca tctgtcgcgg cgccaccaac
     5701 aactgttgca attacatcat tggtaatttc aatggttcca gctgacgttt gaatttttac
     5761 agccatgaat gagcctcctt aacgggtaaa aaactcccat ttttcttcta tcatatttta
     5821 tcatagcgca acttgaaata aaaggcagtt acccaaagta ctcaaaaacc ttgcaaaatt
     5881 tcaggtataa ccttgtttaa tttacaatta ttcttctcaa ttgtcaattg tatctatgaa
     5941 aatgtacttg caaattccca ttctatatga tatgttagtc tggtatgagc tgaattattc
     6001 tattgctcca aatcaaggca aaggaggaaa ttaccatggc aaaagtttgt tactttacag
     6061 gtcgcaagac aaaaagtgga aacaaccgtt cacacgctat gaactcaaca aaacgtactg
     6121 ttaaacctaa cttacaaaaa gttcgcgtta tggtcgacgg aaaacctaaa aaagtttggg
     6181 tgtcaactcg tgctttgaaa tcaggaaaag ttgaacgcgt ttaattgaat gatacttgga
     6241 ccgttcagat agtgaacggt tttttattta ataaaaattt ttaagggtgg gacttttgat
     6301 tcctaccctt tttttgcgtt tatttttgtt atacgaacaa attatgaagt tatttattct
     6361 aaattttatt tttttgtgaa aactagactc tttttaagca gagaaaaaca aggatatgat
     6421 aaactgaata caacttcata gaaatttggt ggaaaataaa tatgaaaaaa aatttaaagt
     6481 cattctttag agaaaacgga cgctacgcag cagctagttt cctgattccg tttttgattt
     6541 tagcacttgt ttatctaagt atcggaatct atcctggaag cagcagaagt attttggcaa
     6601 gtgatgcctt ttcacagttt tctaattttc atgccagctt ccgaaatgtt cttcttggaa
     6661 agcaaagtat tttttataca tggaatgctt ctctaggatt gaattattta tctttaattt
     6721 cttactatct aggcgggatc ttcacgccac tggtgttctt ttttccaaac cagatgatgc
     6781 cagatgcact gtatgttctg actttgctta aggtcggctg tgccggattg gctttttggt
     6841 ttcttgcacg tacgttcaag attcctcgct gggggcatgt tgcacttagc gtcagttacg
     6901 cattgatctc atttatcact gcacattcgg agatcatcat gtggctggat gcattttttt
     6961 atcttccatt agtgattttg gggattcatc gtgttatgga tcttaaacgg ccgaaacttt
     7021 tatttatcag ttatttactc ttgtttttat ccagttttta tatgggtttt atgattggcg
     7081 tattttcttt cttatatttt atcgcccgtc tattgacaaa ctggtcagaa tataaaaaaa
     7141 cgattgttcc ttatggtatc acttctttac tggcaggcgg cgcttcaatg atcattgtat
     7201 tgccagcagt gctggatctt cggtcaaatg gcgaggcact gagtcagatc actacgttga
     7261 aaacagaagc gacttcttat ctggatctaa tcatgaaaaa tatgattggt gtttatgaca
     7321 cgacaaaata cggatcgatt ccgttcgttt acgcaggtct gctaccatta gtcctctgtc
     7381 tgttttattt tgccagtcgg aaagtaccgt tgaaaaataa atttttgtac ggcagtcttt
     7441 tcgccatact tatcgcaagt ttttatcttg ttccattgaa tttgttctgg catggtatgc
     7501 atgcaccgaa tatgttttta ttccgttatg cctatctgtt ctctttctta gtgctcctgc
     7561 ttgcaggaaa aggatgggaa aagatagaac aagacgatcg tgggctgttg ttaggaatca
     7621 ttttggtcgt agctgctctc tttgcggtgg catggggcat caaacctgaa ggaagctaca
     7681 cctatgtgac gatgacttcc tttgtattga cagtggtttt cttagggatt tatgcttttg
     7741 ctgtgggatt ttatcagcat aaaaaacttt cattcaatca tctgagtata ttgttattat
     7801 tgatcatgtc tgctgaagca ctggtcaata cgaacagtat gatccatggc attcttgatg
     7861 attggaatta tgcttcccgc agtttatata ctgatcctta tcctgacttg aaagctcttg
     7921 taaatgaaac aaaaaaagat tccgactcgt tttaccgctt ggaaaatctg gatccagtat
     7981 ctgcaaacga ttcgatcaac tatggttaca gtggtatcag tttgttttct tctataagaa
     8041 atcgtaattc ttcctcttac ctggataaat taggtttccg ttcaagaggg acaaatttga
     8101 atatccgtta taacaataat actttgctga tggatgggtt tacaggaatc aaatataaca
     8161 tttctaaaaa cgaaagcacg ttcagtaaat atggcttccg ccaagctggt caaagcggcg
     8221 actatacact gtatcagaat gtcaatgcat tgccactagc atttactgca ccgttgtccg
     8281 ttaatgatgt tgagcagcca gtgacagata atttgaccag tcagacaaac ttgatcaata
     8341 cattgtctgg tctaaatgaa cagtatttta cattctatac acctactttg aagaatcagt
     8401 tgaatacgac gatcacaaag acaacgactg gcgtcactta ctcagaaaca gcagataacc
     8461 agccaaaaga gctcacttgg gaagtccatg tgcctgcaaa tacacaagct tacttaagtt
     8521 tgttcccaac caattttgcg caattagaaa gttctagtgc gacgatcact gtcaatggta
     8581 catcgcgaaa aacgcaaatc aacatttctg gtcaatacta tgatcttggc tattatcctc
     8641 aagagacaac agtgacattt actgcaagtt tttatgggac gaaagaaata agtttcatgg
     8701 agcctaaagt cgttggtcta gatgttgtag cctatcaagc cgcaatgaat cagatcaaag
     8761 aaaaaggtgt cgaaatggaa gccactggca gaaaagcttc tgggactgta aataccgctg
     8821 aagattccat gttggtgaca actattcctt atgacaaagg atggaaagct aaaattgatg
     8881 ggaaaccagt caaggtcagc aatttcaaag acgcattctt gatgataaaa gttccagcag
     8941 gtaaacatac tgtcactttc agttacttgc ctgaaggatt tacgataggt gcaatccttt
     9001 ttgtcctatg tattgcattg tttattcttt atgtctggta ccttgaccgc aatcgcccct
     9061 tttatggcac gattgatcca gcaatagaag aacaaccacg aaacacacac agaagaagac
     9121 gtagaaaata aagaaacgat ggaacctgtt cagactgtcc gcaccaaggg caatcaaaac
     9181 aacgttccat cgttttttgg ttacagaaaa aataggtaac aaaaagatga ttcacttatc
     9241 aacgcctcgt ccacaagtca cgatattcat caaagatgag aagctgtttt ttgaaattgc
     9301 tctttgtgac tcagagttaa atgacaggct cacgacctct ttcttaaacg gtgttcaatc
     9361 agctgacctt cggtattagc tcaacaaacg gcaaaatatg aggagctatt ttcccgtttg
     9421 tttctctaat actcaggtca aaacgctgat ttcacgacct cttccttaaa cggtgttcca
     9481 gaagtagctt cttcgaaaat aagccgaaat tttgcaaaat tttgagaagt aattttcgta
     9541 aataccttct tatttcttga agctgaccac ttctatcata acctcttatt catatttcac
     9601 ttcatacaaa taaagtcctt cagggtgtgc tgtaggtcct gctgcattac gatctttttt
     9661 cgcaataata tctgggatac tatcttcagg caatcggcca ttgccaattt tcaaaagcgt
     9721 tcctactaag atccgtatca tcttatacaa gaagccattt cccctaaatg taaacaacaa
     9781 ttcgtctttt gtttcattta cctccatctt agcctcgtag atcgtacgta ttttatcctc
     9841 tatcgagctt cctgatgcac aaaaagaggt gaaatcgtga gtccctaata aatcaggaag
     9901 cgcccgctga attttttcca aatcaagtgg gtaaggaaaa taacttgcat aatggcgtcg
     9961 aaaaggactg cgtggtttcc ctatatccac tcgaaattga tacgtctttt ctttgaccaa
    10021 atatcttgcg tggaagtctt ctgaaacaat ttctacttgt cttacagcaa tatcctctgg
    10081 tgactgggta tctagcgcga aacgcattcg ttctaacgga cgctcctctg gataatcaaa
    10141 atggattact tgcccgatcg catgtactcc ggcatctgtc cttcccgaac caaagaccgt
    10201 gatttctttt ccatttgcca ttttttgtaa tgttttttct acttcttctt gaacagtacg
    10261 cccatttggc tgtttttgga atccattaaa atttgttccg tcgtacgcaa tgattgcttt
    10321 gtaacgtacc atgtttttct ctcctttgtt cctaaaaggt tatctggaat aatgattttt
    10381 ctataaaatt
//
LOCUS       DAYYBH010000092         9994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000092 DAYYBH010000000
VERSION     DAYYBH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.095"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            253..732
                     /locus_tag="ACHAUG_002497"
     CDS             253..732
                     /locus_tag="ACHAUG_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8481554.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            765..2993
                     /locus_tag="ACHAUG_002498"
     CDS             765..2993
                     /locus_tag="ACHAUG_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8481555.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3122..4765
                     /locus_tag="ACHAUG_002499"
     CDS             3122..4765
                     /locus_tag="ACHAUG_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8481556.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4838..5383)
                     /locus_tag="ACHAUG_002500"
     CDS             complement(4838..5383)
                     /locus_tag="ACHAUG_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8481557.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5406..5891)
                     /locus_tag="ACHAUG_002501"
     CDS             complement(5406..5891)
                     /locus_tag="ACHAUG_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8481558.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5881..6693)
                     /locus_tag="ACHAUG_002502"
     CDS             complement(5881..6693)
                     /locus_tag="ACHAUG_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8481559.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6832..8364)
                     /locus_tag="ACHAUG_002503"
     CDS             complement(6832..8364)
                     /locus_tag="ACHAUG_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8481560.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8473..9504)
                     /locus_tag="ACHAUG_002504"
     CDS             complement(8473..9504)
                     /locus_tag="ACHAUG_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8481561.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3328 a         1845 c         1923 g         2898 t
ORIGIN      
        1 caaaaaagat gttggtgaag ttcaccaaca tcaaaaagta tagagccaaa atattccaac
       61 atcatcttgg actttctaca tgttactaat gagcgtgtaa aagaacgaga tggtgttttt
      121 atttctatca atgaagctct ttttctttgc aatcctcact tcaatcctct ataatgtcgc
      181 atatagttta gtaaatgagt aaactactaa tttacttatt tactaaaaat aaaaggatga
      241 ggagttaaaa taatggaaat accaacaaat ttaaaacata aaccagtaat cgttgtagaa
      301 gactatgata aagttgatgg aagaaatgca ttaaatatgg atgcaaaagg gctttcactg
      361 ggccttgctc agtggaatga tcgtggaaaa gtcgatatct caggcaaggt atggcgacat
      421 actggagaaa aatggtcacg acagtcggaa gaattaccaa tcacacgtat tttagattta
      481 gcgatcttag ctgcgcaagg aagtttatac ttccaagatg cttatcgtta tgaaaaattt
      541 tatgatccag aaaatccagt cgtagacatc attggattac aagggaaccg aatgacggta
      601 gaagtagata ccaaaaatcc aaagatcgat gaagacatca ttttgtatta cgatacacta
      661 caaaaagacg gagaactgat tggggaacga ttccgtattt taaaacgtct tttagatgag
      721 ctaggatatt aatagacaga gatgatcaaa aggggaagag aagcatggag aatttctcac
      781 taacagaaaa ccgaaccttt tcacggctag aaaagcaaat gatctggaag aaaccgaaaa
      841 gccatcggac aagtgcagaa gaatggcgga tcgcctccga aatcaaaggg aattggcaag
      901 atcctgaaat gaaaatcagt aacgtacttt tggaaggaga tgccgggtcg gggaagacac
      961 aattagccaa agcactatct gctgatctgc aacttcccta tacaaaagtg acctgttttg
     1021 cagatatgga caaatcagat gttttcggag ccttattacc tgtagtagat tctaataagg
     1081 aacaagacca agaattacta gaagctattt accaaacaga tacacttgaa gctgtactga
     1141 cactgatcca gaaccattac ggcattgatc ctttttctgc taaagaaaaa ttagcagagc
     1201 tagttcaaag gatcgaagct gattatccag atgcaatcca ttacagctat tacccttctg
     1261 aaatcgttcg agcgattgaa aaaggctatt tattagagat tcaagagccg actgtcatcc
     1321 gagatgcctc tgttcttgtg gcacttaact cagcgctcga accaaatggg atgttgaatt
     1381 taccgacagg aatcattcgt cgccatccag attgcgtcat tgtgatcact acaaatcgaa
     1441 attaccaagg aaatcgccca ttaaatgaat ctttaagaga tcggatgcaa catgcggaaa
     1501 aaatggatct tcctgcctta gaagtcatgg cagaacgtgc gatggcgaaa actatgatcc
     1561 aaaaacaaga acttctctta aagatggcag agattatccg tttgttggat gaaactgcaa
     1621 aagctaacgc catcaaaggt gtagcaggaa tgcgttctta tttttattgg gcaaatacct
     1681 ataagcaagg acaagattta ctccagtcga tttatccaaa agttttgtat aaattatcca
     1741 ctgattcaga tgaattggca attcttgaac aagctttgtc tgacagtgga ttgctactgg
     1801 aacttcagga catattgaga aaagaaaaat ggggtcatcc tcaatctgag catacaaaag
     1861 aaagaaccat tagcgccgaa gaagcaaatg aacgaaacat cactgaaaat gctgaagaaa
     1921 ttctgcgctt agatgaaaag aaatcgttgt ctgctgaaga accaaatgag ggagaagaga
     1981 aacaagaaga aacaatagaa aaaaacgaga ttcctcctca aatgcaaaaa gaacgcagta
     2041 gcgatatcga aggaaaaaat caaaaagaac aacaaacagg agatgattct caagaaatgt
     2101 ctgcttctga catggaagca catgataagg aaatcaagaa gcaactgaat aaagaagcgc
     2161 gacaaatcat gaaggaaacg attcatgaaa aagaaggtct catcgttcac cgaccagctt
     2221 tccaaggaaa aacgccggaa gcacgtgagc tacacatgca agtaatgcca atagtagaat
     2281 cacttagccg gcaaatttta gagttactgg ataatgaaca aacagagacg taccaaaaag
     2341 gaaaatacga aggtcagcgg ttcaatgcaa gcaaagtagc atatggtgat ttgagaaatt
     2401 ttgacaaaaa gaacccgcca catgagcaac cttctttagc ggtagctgta cggatcgatg
     2461 aatctgggtc aatgatacgg gatgatcgga tcgaagcggc aaaaaaagca gcgattgcca
     2521 ttgccgaatt ttctaagaaa gtcgatatcc cattgttgat ttacggagat acagcagacc
     2581 gttcgtcaag agaaaagact tctttgtttt cttataaaga atttgaagac ggatttcaat
     2641 ggttaaatga aaaattagtc actatgaaac caaggcaaaa caatcgtgat ggtgctgcac
     2701 ttcgtttagc agcagaaaaa ttaaacagac aatccgcaac aaccaaactg ttgcttaata
     2761 ttagtgacgg tcagccaaaa gcgttgccgg attatacagg caaaaaagca aaagaagata
     2821 tccagtctgt tttgaaagaa tatgaacgtc aaggaatcct atttgtctcc gcagctattg
     2881 gccaagacaa agaagaaatc aagtcgatct acggtgaaag tcgttttgta gatattactg
     2941 acttgaatga atttcccaag caaatgattc aactgattgc ccgctatcta tgataaaata
     3001 aagctcaacg aacgcactta gcagattcgt tgagcttttc gtttctagtt accggtaaca
     3061 aggatacttc taaaatagaa tcggttataa tcaagaggga ccgttaaagg agtgaaacga
     3121 tatgatacaa gaacaaaaat ggtggaaaga agcagtaggc tatcaaatct atcctgctag
     3181 tttcaaagat agcaatggcg atggtcaagg agacatcaat gggatccgtg aaaaacttaa
     3241 ttatttgaaa gatcttggta tcggctttat ctggatcaat ccgatctatc aatctccatt
     3301 tgtcgataat ggttatgacg taagtgacta tcaagatata gaagagagat tcggaacgat
     3361 ggaagatttt gatttattgc taaaagaagc acatgaatta gggatcaaag tgattatgga
     3421 tcttgtcatc aaccatagtt cggatcagca tcaatggttt gaagaatcga aaaaatctaa
     3481 agaaagcccg tatcgcgatt attacatttg ggtcgacggg gtagatggca aagaaccgaa
     3541 taattggaca tcgattttcg gcggttctgc ttgggagtat tctcatgaaa ctggacagta
     3601 ttatctgcat gtttttgcaa aagaacaacc agacttaaat tgggaaagcg agaaattgaa
     3661 agaagaacta ttcaatatga tccgatggtg gttggataaa gggatagatg gatttcgttt
     3721 agatgcgatt tctcatgtaa aaaaagatga atattcagta aaagccacag agaacccttt
     3781 ctcacccttt caaaatgtct caggaatcga agaacatttg accgatttga aacatgtttt
     3841 tgaagagtac gatatcatga ctgtcggaga agcaagtggt gtcactgcag aagaaggacc
     3901 acaatgggta gggaaagacg gttatttcga tatgatattc gaatttgatc atatccatat
     3961 ctggcagcaa gaaaaagaag ggcaattaga tgtccttaaa ttgaaacatg cattaagcgc
     4021 ttggcaaact tcattggatg gtatcggatg gaacgcttta tatatggaaa accatgatgt
     4081 tcctcgtgcc gtttcagtgt ttggagatac tcgtcccgat ttttgggcta tgtctgcaaa
     4141 agcaatcgct atgatgtatt tctttttgca aggtacgcca tttatttatc aaggacagga
     4201 aatcggaatg acgaatatgc cctttgaatc gatcgatcaa gttgatgcag tagattcgaa
     4261 aagactttat aaaaggctgc ttgcggaagg taagacaaga gaagaagcat tggatatcat
     4321 tcgtgaaaca acgagagaca acagcaggac acccatgcaa tggacttcgg aacaatatgc
     4381 tggtttttct acacatgaac cttggctgat cactaatcca aataccaaaa cgatcaatgt
     4441 agaacaacaa gaatatgaac cagagtcggt cctgcaattc tataagaaca tgatccgcat
     4501 tagacaaacc aataaaggtc tgatatacgg ttcttataaa gaatatcttc atgaacatcc
     4561 acaactttat gtctatgaaa gatatttaga agatgaacaa tatcttatta tggtaaactt
     4621 aacagaatca cttgctgact atgaacttcc aaaagaagca gatcagtcct ggacattact
     4681 cttgtcaaat tcctctagcg gagaatttga agctaaaggc atactagcac cttatgaagc
     4741 tcggttatat aaaacaaaca agtaaaactt cctcgttaaa gttcattagt tggactttgc
     4801 gaggaagttt ttcagattgc tctgtcaatt actcatttca ataattcgct ctaggaccac
     4861 cgatatattt aggacgagag cgaagggctg tcacgtgagc ttgtccaagg gttttcaaac
     4921 gaggtctgac aggtacttgg acatgcttga tatgcatacc gattgatgtg tcaccgatat
     4981 cgataccggc ttgtccaact acgtgttcca cttcgacagg atcattgaac tggtcaaaag
     5041 cagcaacaga acaagcacct ccagcatgta gtgcaggaac aacagagaca atttcgaaat
     5101 tttttgcttc tgccactgtt cgttctacta ctaatgctcg gttcaaatgt tcacaacctt
     5161 ggacagctag cgcaatttct ttaggatcca acaactcttt taacgtacgg atgatccatt
     5221 gaccaacttc cgcactggaa ttttttccaa tatgtcctcc aacgacttcg cttgtgctgc
     5281 atcctaaaac aaaaagatca ccttttttca agtgtgcctc agccaatatg tcattaacta
     5341 tttcagtcaa ttggtctttt aattcttttt ctgacaactt cattttctaa aaccttcttt
     5401 ttcaattaat attgcttccg tgcttgaata cgttgaagac ttgcagttat aggaaaggca
     5461 agtcccattc caatcagatt ttggaagatg tttcctggta tagaagccac tccagccggc
     5521 caatcatata aaatccaacc ggcgatacca tagccgatga tcataactaa gcttcctaat
     5581 aaaagcccta atccccacat acgagtagaa gatttgctga aagaaccagc aacatatcct
     5641 tgtaacccgt ggatcactaa tgaaaaaact gcccactgcg gatatcctga aatcaaatcg
     5701 atcaatagac cactggaagc accaacaact aatccacccg ttggaccata aagaaaagca
     5761 gcggtataga tcccggcctc gcataatgtc acaaatccat tcgtagccgg tacagggatc
     5821 aaaattgtta atgaaagggc aactgtcaac gcaatcagca tagcatagat cgttaattgc
     5881 ttagttttca tttctcattc ctcctttttg aataccgcca gaccagacac ttccggtatg
     5941 atcgttcatc agcacgccat tagaaataca atgggcaaca aattttttac tttctgctac
     6001 tgctttggta aaatctgccc ctaacacaag attcgctgtt attgcagaag aaaaggcaca
     6061 accggccccg tttactgttg tagtgtcgat ttttttatga gaaaatacct cataatgatc
     6121 aattgaatag agaagatcca cagcttcatc tccagccatc cttgtgccgc ctttgatcac
     6181 aatgtttttt gttcctgaag cagtgagttc tttagcgaga tggatcattt ctgttaacga
     6241 agagggaaga gcttgaccag aaagtaaagc agcttctgcc aaattaggcg taatcatcgt
     6301 aacaagtgga aaaagttctt cgatgagctg cgaaatatac aattgattga tcacagcttc
     6361 agtttctttg aatgccaaaa cagggtctaa aatgattggg atattttgtt ttgtttttat
     6421 gaagtctcga acaaccatga ttgcttcact tgaattcaat aacccaattt tgataccatc
     6481 taatgaagtc attttatcaa tcgaaactaa ctgtcgttta agcaattcca aagatataga
     6541 ttccagcata aatcgctcat taatatctag tgcccctata gaagtcaagg cgcttagtcc
     6601 gaatagacca aaattctcaa aagtttttaa atctgtttgg attcctccac cagcaaatgg
     6661 atcagaaccg ccaattgtca agattctttt cataacattt tcctccatga ataagagtat
     6721 aacgaaattt tcaaaaaaga aaaacagcca attggcatat ttttatctca tccaatttaa
     6781 aaaaacaaaa agaaaagaaa gaccaaaatg gatctttctt tccttaaata gttaggactt
     6841 ttgtgatttt aattctttct cgatttgaag ccgttgcact ttcattgtag ctgttcgcgg
     6901 gattgcatca aaatcacgga tgatagggac gttcaagcga ggaagttcat ggacttgctc
     6961 ccaccatcga ttccaatcca tttctttatc aggagctaaa gcaataatcg gttgtggaga
     7021 atgattttta tcccttacaa tcactacttc tgccaaaaaa tctaacgaat ctaataaaaa
     7081 gtcttcaata gctaatgtac tattgatcgt ttcgatcaaa tccacttgcc gatctttcaa
     7141 aaataggtgg ccatgcttat ccataaaacc gtaatctccg ctatcccacc aatcgccata
     7201 aacattttcc tggaaccgtg catcttcctt gtaataagtc aaagcccgtc ctttagacag
     7261 taattgtata tgaccatctg tgttttcagg taggacattc ccaactgaat ctgtaatccg
     7321 agcgcttgtc atgtcttcca gtccaacgcc catatctctt gcatcacttg ttttcaaact
     7381 ctctagtgta tgagccttaa gaatcattgg tccgcattca ctttggccat atacttgaag
     7441 aaataccgca tcctgtgcca tcgacgttcg caaaaatgtc ttcattgtct ggttgtttat
     7501 agcatcaaaa gtggagtgat agtatcggat acccgaaaaa gcttctggat gctccttagc
     7561 agtaaaacgc cactgaacaa agttattcgg atgggtctct agtgcaattg gccggtgagc
     7621 ttccaacata tgaacaactt ttgaactttg tgcgtttgct aaaggcatca taggaaatcc
     7681 catggacatc aaagaagaga taccaatatt gaatctggaa tgcactggag agatgtgaaa
     7741 agcgattaat ttcttttccg aaatttttgt gaaaactgtt ttttgccatt tcgtccgcca
     7801 tcccatagaa tgagcagaat gacaaataag tttagggata ccagttgtac cagaagtatg
     7861 ggtcatataa gaaatttcat ttttgtctaa aaatgattga gaaacagaca aagcaggttg
     7921 ttccattaaa taaagaatag gaatctgttt tgattttgtt ccatttgaaa tttctctcac
     7981 tcgctttttt gtctcatcat caaataaaat gaaagggtct tctaaacgat cgataaatac
     8041 ttccattgtt gttgttggta gatgataaga agtcatgaca ggcactgcac caaggtaaga
     8101 cacagaaaca gctagaaggt atgtgtcaaa agcggaactc ttataaataa taactttgtc
     8161 tccggatttc actccaagat gagccaattg ataagcccgt ttcaacacac tttcgcagca
     8221 ttttttgtaa gttgtttcaa gtcctaattc tgggaaagct gaaagtggct catcaaagat
     8281 aatcggggtc tcggaaaagt tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg
     8341 ggaatacgtt atggtctcca tcattcacac tccattatct tttgttaagt aatatacaaa
     8401 ttcaagataa tcgtaacata atcctttata taataacata gtttattgtg aaaaaaagat
     8461 catacatttg ttttatatgg aagaaacccg aacgaatgcc gctaattcgt gataagaaaa
     8521 atcaaccatc ttttcccaga tatcttgaaa agaatgggat gagaggtatc gattaccaca
     8581 taaataattt accgcgtcag catcatctaa atatagttct gcataataac gatatgtttt
     8641 tctgcatagt tctggcgata cgtttttata tactgtaatt ttgctcaaaa tttggcgaat
     8701 agtccgagga gaaatttgct gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt
     8761 attttgtgct gttaacttaa aatagttctc cagaaatggc atcgcttctt ttggtattgg
     8821 tactttattc agtctgtttt cttttctatt cagattaaga atctgcttgg tcaaagatac
     8881 atctttacgt ttaagttcta ctacttcatg gatctttaat ccgcaataca gaagaatggc
     8941 agtaattgca aggttcctcg gtctattctt ttcttcaaag ctagcttctc tgcttgtttg
     9001 aaagccttca atcgggataa acaaatgctc aaaatctttg aaaagttctg catcgatata
     9061 tcgcggagct gctttggttg tttccggtaa acttgtaccc aaaagagaaa tctcttcttc
     9121 aatcagtcct ttttgataca aaaatcgtaa aaaatgattt aatgaatatt gcttccttga
     9181 aatcccgtaa tagctatttt caaacaattg ctccgtttct tggttcgctg tagtttggaa
     9241 cgaacgagta tgtcgataga ttttagctaa gaaattctga taatcttgtt tttctaaatg
     9301 gataatcagc tgatcatcca cttcttcaga aaatactttt ctcaaataat cagtaaacag
     9361 atgaaaatct tttgcgtatt catagcgact gcctaatgaa agggaagaac gattttcttc
     9421 taaaaactgt tgaaagacgg aagggagcaa gtgtgtacgt tgctttaact tttcttgcag
     9481 ttgttttcta gttttgctgt ccattacaaa ttccctccaa tagtgttcat tttctatatt
     9541 ttaccgcatt tcatcaaatg aagatagaca gtttgtcttg atcttaggaa gcaaattttt
     9601 aactaagtac tagctaaaag atatgaagta agaagataca tttttagtta ttttattagt
     9661 gcagtcattc ttttgatctt ttaattgatt tatcctcttt ttagctatgt ggttacttca
     9721 tgagcaatga aatggatttg gttcgttcta atggcaggag gctctttctt ttatttccga
     9781 aaaattagaa aacatttttt cagaatcgat tggctctcca tttattcaat aatggtttgt
     9841 ctaacgatat tgttgggata attcttaaag aagtattaga aatagttggt acttcattac
     9901 tttacatgaa gatttattct atggtaggag caattatatt gagaattaat ccttttttat
     9961 ataagttgtt tgcttattga tggattggat aaat
//
LOCUS       DAYYBH010000093         9982 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000093 DAYYBH010000000
VERSION     DAYYBH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.096"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..1238
                     /locus_tag="ACHAUG_002505"
     CDS             117..1238
                     /locus_tag="ACHAUG_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8481562.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(1320..3092)
                     /locus_tag="ACHAUG_002506"
     CDS             complement(1320..3092)
                     /locus_tag="ACHAUG_002506"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8481563.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3095..4807)
                     /locus_tag="ACHAUG_002507"
     CDS             complement(3095..4807)
                     /locus_tag="ACHAUG_002507"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8481564.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4922..5590
                     /locus_tag="ACHAUG_002508"
     CDS             4922..5590
                     /locus_tag="ACHAUG_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8481565.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5657..6445)
                     /locus_tag="ACHAUG_002509"
     CDS             complement(5657..6445)
                     /locus_tag="ACHAUG_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8481566.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6528..8000)
                     /locus_tag="ACHAUG_002510"
     CDS             complement(6528..8000)
                     /locus_tag="ACHAUG_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8481567.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8130..9323)
                     /gene="metK"
                     /locus_tag="ACHAUG_002511"
     CDS             complement(8130..9323)
                     /gene="metK"
                     /locus_tag="ACHAUG_002511"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8481568.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(9321..9400)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(9589..9660)
                     /locus_tag="ACHAUG_002512"
     tRNA            complement(9589..9660)
                     /locus_tag="ACHAUG_002512"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9625..9627),aa:Glu,seq:ttc)
     gene            complement(9672..9745)
                     /locus_tag="ACHAUG_002513"
     tRNA            complement(9672..9745)
                     /locus_tag="ACHAUG_002513"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9709..9711),aa:Ile,seq:gat)
     gene            complement(9758..9828)
                     /locus_tag="ACHAUG_002514"
     tRNA            complement(9758..9828)
                     /locus_tag="ACHAUG_002514"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9794..9796),aa:Gly,seq:tcc)
     gene            complement(9833..9905)
                     /locus_tag="ACHAUG_002515"
     tRNA            complement(9833..9905)
                     /locus_tag="ACHAUG_002515"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9870..9872),aa:Phe,seq:gaa)
     gene            complement(9909..9981)
                     /locus_tag="ACHAUG_002516"
     tRNA            complement(9909..9981)
                     /locus_tag="ACHAUG_002516"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9946..9948),aa:Asp,seq:gtc)
BASE COUNT         3218 a         2114 c         1657 g         2993 t
ORIGIN      
        1 aaagctgaac aatatagacg atctttagaa ttgtcaaaag attaaatata taaaaagcct
       61 tcgggctttt tcttttaaca attaatagga acgtatattc gaaaggaata gaaataatgt
      121 ggttagaaaa aataaatgac aaaaaattta agtacaccga acgttatacg gatcccttaa
      181 ctgaaaaaaa gagaaaagtg tccgttactt tatctagcaa ttcacgccaa gcttggaatc
      241 aagctaactt acttttgaat gaaaaaatcg cagaaaaaat aaaacgaaat gaagaattac
      301 cactaacttt tggagaatta aaaactaaat gggatgaaaa atacaagcca actgtaaaag
      361 aaagttccta tcgaacaact caggtttatc tatcattaat ttcaaaatat ataaaagatg
      421 atgtacttgt aaaaaacgtt aatagtaatc ttatacaaga tatgctagat tactactatt
      481 ttgaaaaaaa tttatcatat aattatgtaa tacatttaaa aacttttaca ggaatgattt
      541 ttaaattcgc caaccgtcga tatggattaa aatataatcc cgtaaatggg gtttctttag
      601 caagaaaacc taagacaaac gaagaaataa aaaaagaaaa agatggttat ttatcaaaag
      661 aagaagttca aatgattgct agtaaacaaa aaagttcaca tcaacagtca cgttattctt
      721 taattactaa atttttgttt ttaactggac tacgttatgg tgaattgata tctttgactg
      781 aatctgatta tgatggtaat aaaattacgg tttctggtac ttatgattac gagttaaaaa
      841 ttaaaacaac aactaaaaat acaggttctt atagaaccat tgaattatca agtaatgcta
      901 aagaaatcat agatcaatta attgaagaaa acaaattaat taagaataat aaagataaat
      961 acattttcat ctctaaaaat ggtaacccga tatctattca agcatataat caatctttaa
     1021 gagcagtatc caaagaatta aacctagata aaaaagtttc ttctcacatg ttaaggcatt
     1081 cccacatttc tctacttaca gaattaggaa tcccattaaa agccattatg gatcgtgtag
     1141 gacatgaaga ttcaaaaaca acattaaaga tttatactca tacaactaaa aatatgcaaa
     1201 atcaactagt agaaaaacta ggaaaaatag aaatttaaat tgcccctttt ctgcccctta
     1261 taaaaacaaa caacataaaa tatataaaaa gaaccccgtt ataacgaggt tcttttaatt
     1321 taactttcaa atacaaattg attatgataa agttccgcat aaaatccatt ttctgctaat
     1381 aactcgtgat gactgccttc ttcaatgact tccccatcac gcaagacaac gattctatct
     1441 gcattcaaga ttgttttcag ccggtgagca atcacaaagc tggttcttcc tttgattgct
     1501 tcatccattg ctttttggat ccttgcttct gttaccgtgt ctacattact tgttgcttca
     1561 tccaaaatca ataaagatgg atttgtgatg atcgttcgag caatactgat caactgcttt
     1621 tgacctgtac tgaataagtt attttcttcg gatatctctg tatcataacc tttttctaac
     1681 gtcatgatga agtcatgaat attcgcttgt tttgctgcat tgatgacttc ttcatctgaa
     1741 gcgtccggtt tgccgaatgt gatattgtct ttgatcgttc cggagaacaa tacagattct
     1801 tgcaaaacga ttcctacatg tctacgtaat gcatcaagat cataatctcg gatatctatt
     1861 ccatcgatga ctactgaacc atcattgaca tcgtaaaaac ggtttagcag attcatgatc
     1921 gtcgttttcc ccgatcctgt tggaccaaca agagcaacca tttctccttt gtccacattg
     1981 atcgagacat ctttcaagac tagtttttct ggagaataac caaaatctac gtgctgcaac
     2041 gcaagacctt tttcaacacc atcaaatgct ttacagtttt ctggtttgac ttcatctttt
     2101 tcatcaaata tttcattcaa tcgtcttgcc cctgtaatcg ctaattggat catgctgtat
     2161 ccagatgaaa tctgcatcag tggttggtaa tattgttgtg aatactggac gaatgtcaca
     2221 actaagccta atgccaccga acgttccaaa tcaccattta atgctaacca gccgccgaaa
     2281 aagataacta tcgctgtatt gaccagtgac attccttgca tcatcggaaa tagtagacca
     2341 gaataagctt gtcctttgaa ggttgcttcg cgaacacttt gattatgctc taagaatcct
     2401 tcaatcgttt cttcttgtaa gccatttgtg atgatgacac gttgtccgct gattttttca
     2461 tccatataac catttagttt ccccacttca tcttgctgaa gatccacata tttccgtgct
     2521 ttgcggataa tcaagacagc tatcaaaacg gctactggag tggaagcaat tgtagcccaa
     2581 gctaactcta cattttggcg gaacatcatg atcaagatcc cgacaagtaa aacagcattc
     2641 gtgatgactt gaagcaaagc ttggttcaaa ctattttgaa tattatctaa gtcactggta
     2701 aaacgactta aaatttcacc gtcttgatga gaatcaaaga aacggatcgt caatttttcc
     2761 aatttgttga atagaccgat acgcattcgg ttcgttgact tccctactac ttgtgtgaac
     2821 aaaatactat agataaagtt ggctgcacta gctagtacat aaaacagcaa gagtttccat
     2881 attacagaaa caaatttgct tttatcgtct acgccttgca tcaagcctcc tacgtaatta
     2941 gctaattctt gaaaagcttc cccgatgtat tgaggtgcct ttacttgtag ataggtagca
     3001 atgatcactg tgataaagat aaagaaaaag gagagtttgt accgcttcaa atagtgatag
     3061 aaaaatttac ttgcttttat tagatcagtc atacttactc ctccgttcct ttttgagttt
     3121 caaagatttc ctgatagaca cggtttccag ctaccaattc ctcatgggtt ccttcgccga
     3181 ctaatctgcc ttcatccaat actaagatgc ggtctgcttt gacaacagaa gcaatttttt
     3241 gtgcgatgac aatcgtcgtt gttcccttca aatctcgatc gagtgcttct cggaccagct
     3301 tttcagagcg agcatccagt gcactcgtac tgtcatctag gatcaagatc tttggattac
     3361 cgataacgcc acgagtaatg gataaacgtt gcttttgacc accagaaaag ttactgctac
     3421 gttcttctat tggtgcttcg taacgatcag ataacttttc gatgaattct tttgcttgag
     3481 caatagaaga agcatgttcc atctcttttt ctgttgcatt ctttttccct tggcgcaagt
     3541 tttgagcaat cgttcctgag aataaaattg ctttttgcaa aacaaaggaa accgtttgtc
     3601 ttaaactctt ttcatttact tgacgaagat ctacaccacc tacttcaact ttgccttctg
     3661 tcggatcaaa tagacgaggg atcaattgtg ctagtgttga cttcccggca ccagtcgccc
     3721 cgacgatacc aaccatttct ccaggtttga tagaaaagct gatatcttta agcgtatctg
     3781 tatcctcacc aggataacga aaagaaacat ggtcaaaagt gacagaaccc gtcaactctt
     3841 gttctggaac gtctaaataa gtaagatctg gttcagcatc taaaatttcc gcaatccgct
     3901 tcagcgatac ggctgctctt gaagtcatca tcatcatcat tccgccaata atgattgcca
     3961 tcataatctg catcaagtag ttcatgaatg aagcaattcc accaattaac gtaggatcat
     4021 ctttaacaag atcgctcaca aaaaagatcg ccccaaccac agccaaattg gcagccagca
     4081 tgaaggacgg gatcatcaca gaaaataatg taccgactat gatgttgtgt tttgttaagt
     4141 cctcacttac tttgctgaat gacttcaatt ggttttcttc ttgtacaaaa gatttcacta
     4201 cacgaatccc tagtaagttt tcttttgcaa tgctattcac acgatcaatc agtttttgaa
     4261 tgatcatgaa gtgcttgccc atccttgtaa atgaaagtgc cgtaatcaga aataccgtaa
     4321 cgataagcgc tacgatgatc caccataatt gcggcatcgt gatcatagct aaaatgaagg
     4381 caccaataaa taagaatgga attcgaaaaa gtgattgcaa agaaatcatc actaaatttt
     4441 ggatttgcgt gatatcattt gttagacgaa caacaagatt tccagcagag aactgttcga
     4501 tatttccaaa tgaaaatgtc tggatctttc gaaatgcttc ttctcggata tctgcactta
     4561 cgccttgagc tacttttgca gagaaaatcg tgttcgttac acctgcggct aatcctagca
     4621 aagccagact gatcaggtaa ataccaattg ttttcatttc atcattatct tctgtaataa
     4681 tagcttccaa tacttgctgt agtaacttag gttgccataa tgctgctgca accatgactg
     4741 ctacggacag caaggaaata aaaacagcta ctttatattt tttcgcatgt cgtaagatta
     4801 atttcattcg ttgattacac ccctttgttt aaattgacag gactaattta acactgtaaa
     4861 attagaccga ctaacaattt accatgttta aaaagataat gcaatacacg aggttgataa
     4921 aatggaaata aagctttctc aagcaatcat cattcaggcc gcctttgata tcctggctga
     4981 aacacaagct ctttcgcagc tttccatgcg aaacatcgca aacagaattc atgttcaagc
     5041 acctgcttta tattggtatt tcaaaaacaa acaacagctc ttgcaaataa tggctgaaac
     5101 aatggaagaa gaattagcgc ttccagatcc aactttgcct tggaatgaac agttgctgca
     5161 gtatatggaa aattactatg acctttatac acgtttccct tgtggggcag aattagaaat
     5221 caatactgtg cccgcttttc cgagtcgatt agaacatttg gagcaaatga tccaattact
     5281 tgttcgtgaa ggattttcag tcacccaaag tcatcaggcc attgcctctc tccacaattt
     5341 attgattgga cagttaatgg atcagcagca tgaacagcag cttcgacaaa aaattatgga
     5401 cggaaatgaa tttttgaaag aaacagttct atttatgcgt aattatgtag ttgaaaatca
     5461 attagaggga tttaaagaaa gtatgcagtc ccatgcagaa ttgaatgaaa aagcactctt
     5521 tctaacgagt gcacaaacct atattgaagg cttatgccaa caaaataaaa gaagagaaaa
     5581 cgagcagtaa aaaagctcct atagagatca ctatgtttgt gatctctata ggagctatct
     5641 ttttaagact ctttttttat ttttcttcca ttagccacgc caatgcacgt tgaatccatt
     5701 ggtcccagaa gatccagtta tgatcgccag gtccttgttc aaaggtgacc gccaagcctt
     5761 gttcctgcat agcagatacc atttcctgat ttgcctgata aagaaaatca gattccccac
     5821 aagccatata aaatcttgga actggttttc cgctttttac tagttctttc aataagtatt
     5881 gcggatcatt ttttgaacct tcgatttgat ccaatggtcc aaagatccct tcccaaaaag
     5941 atgctttacg aaccatcaaa agatcatcca ttcgatttcc tacagaaaca gcgcctgaaa
     6001 gagagattac tgctgcgaaa ttttctgatt ttgcaaggcc tagttttaac gcgccatatc
     6061 cgcccatcga taatccagcg gcatatgttt tttctctttt tcttgttaat tgcgggaata
     6121 attcatgaca aatctcaggt agctcttctg caatgaatgt ccaatacttc atgtcataaa
     6181 ctgtatctgt gtaccagcca agatcagtag atggcatgat gacagctaat ccgtagtttg
     6241 ctacataacg ttcaatcgac gtacgtcttt cccatacact atgattgcca cccattccat
     6301 gaagtaaata taaaacaggt atatccgttg tctcttgctc tgaactggtg ccaatcacct
     6361 tttttgttgt ttgcggtagt atcacattta gattgacttc catttctaac acattcgaat
     6421 aaatatttgc ttgaataaat gccatatttt cgccttcctt agtaagaagt tatttttgaa
     6481 taactttaaa tttgaatgat tcggctacgt acatatctta accgaaacta ctgttttatt
     6541 ttaccacttt tttcatgaac gaaaagcgtt aagaccaaag caatgatcac gagtaacaag
     6601 ccaactagat aaacggaatg taacccatca taaagaattt ttcttaatgg taacaaaata
     6661 tccgcatcaa tatatttagc cgtttgtgga ttcaccagtt tattcatgat atctgcatca
     6721 tttgttaaat ttgctgctgc aagttctcgt ttagttaccc catttatcaa taaaccaaaa
     6781 atagaaacca tgactgtttg accaattgtt cgaactaacg tattaaaaga agttgctaca
     6841 ccaagctggt cacgagatac actattttgt gcactgacag tcgaggtagt catggtcaca
     6901 cctaagccta taccgatgac cgttgatata gcaaaaaacc ataaatagtt acttctgaaa
     6961 ggcataagat acaaccaagc gcccccaata gctgcgatta gtagtccaat tctcaagacc
     7021 caatgagttt tcatttgagt catccacttg cttgcaagga aagaaccgac catccagact
     7081 agtgataatg gtgctaatac cagcccacca attgctgcat tcagccctaa tacaccttgc
     7141 atccacatag ggatataaac atcgattccc atcaaaaaac cactagccaa tgctgcagcc
     7201 aagttgacca caacaaatag tggatttttg aataaatgca tcatgatgac aggatcttta
     7261 gctctttttt cggcaaaaac aaaaatacct aacattacga tactacctag cagcaaagca
     7321 gaaacaagcg gactcattcc ttcgtctcct aataattgga accctaacag caatgagaca
     7381 agcatggcca tcaaactaaa actaccaaaa atatccattg gtttagactc atgtatgatt
     7441 tttggttcat ttaaatatag ccaaatcaaa ataattaata gaattccgat cggcacattg
     7501 ataaaaaaga tccagtgcca gctgatcgtt tcaacgatga atcctcccgc aagcggtcca
     7561 aaaacactgg caatccccca ggctgaacta ttcaaaccta aaacttttgc tcgcttttca
     7621 atagaataaa gatcgccgat gatcgtcaaa gctactggca tcattgcccc agcacccatc
     7681 ccttgaatcg cacgagcagc aatcaaccca atcatgcttt gagataaacc gcaaaataac
     7741 gaaccgataa taaacaataa tgtcccaaaa ataaagattg gtttccgtcc cactttatcc
     7801 gcaagttttc cgtaaatcgg agtaagcata gcgtttgtca gcaaataaat cgaaaatacc
     7861 cagttcatga tttccattcc atgtaatgaa ccgacaatcg ttggcatagc ggttgttacg
     7921 attgtacctt caatagccgt catgaacgtt gcaatgaaaa tactcactgt gaccaaagta
     7981 acatttgttt cttttttcat cttattcctc cctttcatta tgacaggatc taaaaataga
     8041 taaataatta ctatcttttt caagaggtta actgccataa aaaaagctgg aacaaccacc
     8101 acttggcgat cgttccagct gaatataact tattttgcta aacttgcttt caacgcatct
     8161 actttgtccg tatgttccca aggcaaatct acatctgtac gaccaaaatg accgtaagca
     8221 gctgtttgtt tgtaaatcgg acgacgcaaa tcaagcatct cgatgattcc tgctggccga
     8281 agatcgaagt tttctctcac tgcagcaatc agttcttctt ccggaaccgt tccagtaccg
     8341 aaagtattga ttgagatcga aacaggttga gcaacaccaa tcgcataagc tagctgtact
     8401 tctgctttgc gagcaagccc tgctgcaaca atgtttttag caatataacg tgcagcatag
     8461 ctggcagaac ggtcaacttt tgtcgcatct ttaccagaaa aagcaccgcc accatgacga
     8521 gcataaccgc catacgtatc aacaatgatt tttcttcctg ttaatccggc atccccttga
     8581 ggaccaccaa tgacaaatcg gccagtcgga ttgatataat atttcgtttg atcatctaat
     8641 aattcagctg gaataacttc cttcactact ttttcgatga catcatgacg aattgtttca
     8701 ttatctactg catcatcatg ctgtgttgaa ataacgattg tatctacgcg ttccggttgt
     8761 ccttgatcat catattcaac cgttacttga gattttgcat ctggacgtaa atacgttaat
     8821 tcatttgatt tacgcaaatc tgccaagcgt cgtaccagac gatgactcaa agcgattggt
     8881 aaaggcatca attctggtgt ttcatctaca gcaaacccaa acattaaacc ttggtcacca
     8941 gcaccaatct catctaatac gtctttttta tcttcatccc ggatctcaag tgcttcatca
     9001 actccttgag caatatcagg agattgttca tcaatcgcaa ccaatacagc tgctgtatct
     9061 ccatcaaatc cgaattttgc acgtgtataa ccaatttctt ttattgtttc ccgtacgact
     9121 ttttgaatat ctacatatgc cgtcgtagat atttctccaa atactaaaac aagaccagtt
     9181 gttacagacg tttcacacgc tactcgtgct gttggatctt gttttaaaat tgcatccaag
     9241 attgcatcac tgatttggtc agcaatttta tctggatgtc cttcagaaac agattctgat
     9301 gtaaataagt gtctttctac cattataatg attcccccta gttaaattcg gttacaaggc
     9361 atctccgtcc tgcggatcct ttcgggaact tgcaacgaac caagtatagc agataaaaca
     9421 gttttctgct cgtatttttt catttttttg cttaaaaaat ataaaaatat tttacattag
     9481 aactgtccaa aaaagcttag cacaatcatc cataaagata actctgctaa gctttttaaa
     9541 atcattcttt cagataaaaa gaaaaagata ctcaaaaagt atcttccatg acccgtacgg
     9601 gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc
     9661 aataaaataa atgggcctaa atggactcga accatcgacc tcacgcttat caggcgtgcg
     9721 ctctaaccag ctgagctata ggcccataaa aactaaaagc gggtgacgag aatcgaactc
     9781 gcgacaacag cttggaaggc tgtggtttta ccactaaact acacccgcat aatggcgcgg
     9841 gacagaatcg aactgccgac acatggagct tcaatccatt gctctaccaa ctgagctacc
     9901 gagccaaacg gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt
     9961 taacccctac accacggaac ca
//
LOCUS       DAYYBH010000094         9811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000094 DAYYBH010000000
VERSION     DAYYBH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.097"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACHAUG_002517"
     CDS             complement(34..2154)
                     /locus_tag="ACHAUG_002517"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8481569.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACHAUG_002518"
     CDS             2384..3562
                     /locus_tag="ACHAUG_002518"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8481570.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACHAUG_002519"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACHAUG_002519"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8481571.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACHAUG_002520"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACHAUG_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8481572.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACHAUG_002521"
     CDS             complement(4915..6168)
                     /locus_tag="ACHAUG_002521"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8481573.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACHAUG_002522"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACHAUG_002522"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8481574.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACHAUG_002523"
     CDS             7746..9092
                     /locus_tag="ACHAUG_002523"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8481575.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACHAUG_002524"
     CDS             complement(9400..9786)
                     /locus_tag="ACHAUG_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8481576.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DAYYBH010000095         9695 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000095 DAYYBH010000000
VERSION     DAYYBH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.098"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..522
                     /locus_tag="ACHAUG_002525"
     CDS             283..522
                     /locus_tag="ACHAUG_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481577.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ACHAUG_002526"
     CDS             complement(884..1144)
                     /locus_tag="ACHAUG_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481578.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="ACHAUG_002527"
     CDS             complement(1329..1826)
                     /locus_tag="ACHAUG_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8481579.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACHAUG_002528"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACHAUG_002528"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8481580.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACHAUG_002529"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACHAUG_002529"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8481581.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="ACHAUG_002530"
     CDS             complement(4548..5210)
                     /locus_tag="ACHAUG_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8481582.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="ACHAUG_002531"
     CDS             complement(5220..6035)
                     /locus_tag="ACHAUG_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8481583.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6297..6740)
                     /locus_tag="ACHAUG_002532"
     CDS             complement(6297..6740)
                     /locus_tag="ACHAUG_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM8481584.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6874..7212)
                     /locus_tag="ACHAUG_002533"
     CDS             complement(6874..7212)
                     /locus_tag="ACHAUG_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8481585.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7200..7577)
                     /locus_tag="ACHAUG_002534"
     CDS             complement(7200..7577)
                     /locus_tag="ACHAUG_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8481586.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7801..8994
                     /locus_tag="ACHAUG_002535"
     CDS             7801..8994
                     /locus_tag="ACHAUG_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8481587.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9160..9582)
                     /locus_tag="ACHAUG_002536"
     CDS             complement(9160..9582)
                     /locus_tag="ACHAUG_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481588.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2892 a         1935 c         1589 g         3279 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta
     6181 tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag
     6241 caagactgtc atacgtctcg cttttgtatt gaaatttctt caattagaga tacttattac
     6301 tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga
     6361 ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc
     6421 actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc
     6481 tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc
     6541 ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa
     6601 agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt
     6661 tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct
     6721 gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg
     6781 atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac
     6841 ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat
     6901 cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa
     6961 tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt
     7021 gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg
     7081 gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc
     7141 tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt
     7201 tacttgatac attccatttt atttctagat aacacacgat catatttctt gatttttcct
     7261 tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag ttcactttct
     7321 tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata
     7381 tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc
     7441 cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga
     7501 atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg
     7561 acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta
     7621 ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta
     7681 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
     7741 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
     7801 atgacaacgt atcagcgaaa ggtattatta tcgacatcgg cgggaattgc tttagagaat
     7861 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
     7921 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
     7981 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
     8041 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
     8101 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
     8161 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
     8221 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
     8281 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
     8341 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
     8401 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
     8461 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
     8521 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
     8581 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
     8641 ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     8701 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     8761 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     8821 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     8881 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     8941 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     9001 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     9061 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     9121 agctttttca actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat
     9181 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     9241 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     9301 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     9361 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     9421 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     9481 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     9541 tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     9601 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     9661 caatagtgca taaacctgtt attatttctc ttttt
//
LOCUS       DAYYBH010000096         9593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000096 DAYYBH010000000
VERSION     DAYYBH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.099"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACHAUG_002537"
     CDS             complement(<1..641)
                     /locus_tag="ACHAUG_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8481589.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACHAUG_002538"
     CDS             complement(643..1407)
                     /locus_tag="ACHAUG_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8481590.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACHAUG_002539"
     CDS             complement(1415..2092)
                     /locus_tag="ACHAUG_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8481591.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACHAUG_002540"
     CDS             complement(2129..3514)
                     /locus_tag="ACHAUG_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8481592.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACHAUG_002541"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHAUG_002541"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACHAUG_002542"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACHAUG_002542"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8481593.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACHAUG_002543"
     CDS             4453..5310
                     /locus_tag="ACHAUG_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481594.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACHAUG_002544"
     CDS             complement(5391..5726)
                     /locus_tag="ACHAUG_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481595.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACHAUG_002545"
     CDS             complement(5793..7163)
                     /locus_tag="ACHAUG_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8481596.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACHAUG_002546"
     CDS             complement(7241..7777)
                     /locus_tag="ACHAUG_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8481597.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="ACHAUG_002547"
     CDS             8086..9567
                     /locus_tag="ACHAUG_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481598.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt att
//
LOCUS       DAYYBH010000097         8726 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000097 DAYYBH010000000
VERSION     DAYYBH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.100"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(190..669)
                     /gene="pssE"
                     /locus_tag="ACHAUG_002548"
     CDS             complement(190..669)
                     /gene="pssE"
                     /locus_tag="ACHAUG_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFM8481599.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(686..1147)
                     /gene="pssD"
                     /locus_tag="ACHAUG_002549"
     CDS             complement(686..1147)
                     /gene="pssD"
                     /locus_tag="ACHAUG_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFM8481600.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(1161..2411)
                     /locus_tag="ACHAUG_002550"
     CDS             complement(1161..2411)
                     /locus_tag="ACHAUG_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8481601.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(2789..4240)
                     /locus_tag="ACHAUG_002551"
     CDS             complement(2789..4240)
                     /locus_tag="ACHAUG_002551"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8481602.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(4561..5325)
                     /locus_tag="ACHAUG_002552"
     CDS             complement(4561..5325)
                     /locus_tag="ACHAUG_002552"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8481603.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5353..6051)
                     /locus_tag="ACHAUG_002553"
     CDS             complement(5353..6051)
                     /locus_tag="ACHAUG_002553"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8481604.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(6063..6842)
                     /locus_tag="ACHAUG_002554"
     CDS             complement(6063..6842)
                     /locus_tag="ACHAUG_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8481605.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6858..7802)
                     /locus_tag="ACHAUG_002555"
     CDS             complement(6858..7802)
                     /locus_tag="ACHAUG_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8481606.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7848..8627)
                     /locus_tag="ACHAUG_002556"
     CDS             complement(7848..8627)
                     /locus_tag="ACHAUG_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481607.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2482 a         1821 c         1379 g         3044 t
ORIGIN      
        1 gcatacataa aatataaaga tttaaatatt gcatttccac ccttataact cggtataaaa
       61 gtcatctcga tattcttttt ttcccaatta aaattgagca tttgtgtaat tatacttgta
      121 attccacctt ttaccgagct gtcattacca atcataagta ctctttgcat aacgctttac
      181 tccttatatt tatatttttt ctattaatac ctctatcgaa ttaataatcg tttccgtgtt
      241 agatacatac ttgttaaaca tccgaactct tgtttcttta acggcatctt ctaaattttc
      301 taattcataa caaggataga taatattcat ctcatcaaat tgtctaatta actgcagttg
      361 gtgatcatcg acatgctcac catacttagc caatcgagga acagcaatga cccttttacc
      421 tttttttacg gccccaatta tagcacctgt acctccatga gtaatcacca tttctgcttt
      481 ttgcatagtt tcagtaaact catccctatt tagaaatgct ttataggaat aatttagtgg
      541 tttataatca ctataaccac tctgtgcaaa tgtactttcg attattactc ctttttcaat
      601 taaatcatca atagctttta acaatctatc aaattgaaat ttttgtgaac ctaatgttat
      661 aaatatcatt tttttctcct attatttaat atatcccacc caaataaata gcattaggat
      721 aaaattgtag catagtttcc cactgaacat aaaattgatc tgcaaaatga tacatcaatt
      781 ttcctgtctg agtagggctg gttacttttg caaacgattc aatataaatc agtttttttc
      841 ccatcatttt tgttattaag cagataggga tcatagctaa aacacctgta caaatcacga
      901 gatccggttt ttcctttata tatataataa ttgatttgaa taaattaata agtatagtac
      961 acaaaaaaaa cttttctttt ctatttactt gtctcatgta gtacgttttt tcgccttttg
     1021 actcagctga atattctgtc tgttctgtta taacaaaact ctcatacttc tccatcagtg
     1081 gttttaacat cattaactgt tcataatgcc ccccagagga agccgcaaaa catatctttt
     1141 tgttcatatt aacctctcta tcagtcacgc tgaaaaatat ctctagtgta aactttttct
     1201 ttaatatcat ccaagcaggt atcatatcga ttggcaataa tagaatcaca catcgcctta
     1261 aactgctcta aatcgttgat aaccttactt ccgaaaaaag tctctccatc tttcagtgca
     1321 ggctcataaa tcaccacagt cgcaccttta gcttttacgc gcttcataac tccctggatt
     1381 gaactctgac gaaaattatc actgttgctc ttcatggtca gccggtatat accgattaaa
     1441 actggatttt ccctgttgac atcatactga ttatattcag cgtagttgta atagcccgcc
     1501 atctgaagaa cacgatctgc gatgaaatcc ttacgggtac gattagattc aacgatagcc
     1561 tcaatcaaat ttccaggaac atctgcatag tttgccaaca actgcttggt atcttttggc
     1621 aagcaataac ctccatatcc aaaggatgga ttgttgtagt gagttccaat acgaggatcg
     1681 agacaaacac cattgattat ctgctgagta ttcagcccct tcatttcagc ataagtgtct
     1741 aattcattga aatagctgac tcgcagtgct aaataggtat ttgcaaacag ctttactgct
     1801 tcggcttcag tgaatcccat aaaaagagta tccacgttct ccttaattgc acttttctgc
     1861 agaagtgttg caaaaacatg agcagcattt aacaagcgct catcagtcat atcagtacca
     1921 acaataatac gagatggata cagattatca tataaagcct tgctctcgcg cagaaactct
     1981 ggactaaaaa taatgttctt actccccgtt gtctcacgga ctgattcagt gtagccaaca
     2041 ggaattgtag acttgatgac catgatggta tcaggattca cgtccatcac aacctcaata
     2101 actgactcaa cagcagaagt atcgaaatgc tgagtacgac tgtcatagtt agtcggtgta
     2161 gcaataacaa cgaaatctgc ggatttataa gcgctccttg catccaaagt agcagttaag
     2221 tcaagttctt tttctgtaag atacttttca atatattcat cctgaattgg agacttccgt
     2281 ttattaatca gttcaatctt ttcagggata atgtctaccg cggttacctg gtgatgctgc
     2341 gccaatagtg tggcgatact caaaccaaca taaccggtgc cggctactgc gatgttgtac
     2401 tttttcttca caatatctcc tctttcctct cttaagtatg gagaatgtta ctcctacggc
     2461 tattcatcat gtcaaaataa cacagcttag aatgttgata aaataatttt ccagaatgtg
     2521 ataaaatctc tttcatttat aaaccacaca tctaatttta tctattctcc acaagacatg
     2581 tagagtattc tttaataaca agatacgacc ttactaaata cttcaaaaaa ttatcatatt
     2641 taattcataa atataaatct tcattttaaa tgtatgccca aaaatttatc tgtaatgcgt
     2701 catcaaaatc tagattccaa atctttatca atttttatcc actcattcta ccactttagt
     2761 aatattttct atttataaca ctttcgtcct acattgatcc ccccctctga agtacagttt
     2821 ttactgtctt aaataaaatt ttaaaatcta accccatact ccaatttttt atatattccg
     2881 tgtcaagttt tgctacttct tcaaaatcag taatatcact tctaccactt acctgccaca
     2941 ttccagtaat tcctggctta atagcaattc ttgcacgatg atgataatca tattcttcat
     3001 actctgattt aaggcatggt cgaaatccta taaggctcat ctttcctgtt agaacgttga
     3061 aaaattgagg gaattcatct aaagacgtgg atctaataaa ttgaccgatt cctgtctttt
     3121 ttgttccatc tggaagtatt ttatttccga taattcttgg atcaaactcc atcttaaaca
     3181 taagattatt gctcatctta ttttctttca ttaattctac ttttctttct tctgcatcca
     3241 tatacatact tctaaactta tacatcttga acggtttacc attctgacct atacgttctt
     3301 gtgcaaagaa aattgggcct ggtgaattaa tataaataag tggagcaata aagataaaaa
     3361 taattcccgt tgcaatacat ccaacaatac ctgcaagaat atcaattgtt cgttttataa
     3421 atgcctgttg agttgtcata acattgatac ttgttgtaag tactgtatat tctcctatac
     3481 gaccaataga ttgttttccc aaagcactat ctgatacttt tgcaagattc aaatgtacag
     3541 taaggcccat ttcagagcat cgttcaataa attctttcat acacgaagta cttttattca
     3601 aaataataaa gacttcatct acccatccct gacataagta atccgctgca tttttggaat
     3661 cagaaacaac tgtcactccc tcaacatctt caccaatcat gcttttatct atgataatga
     3721 caccatttat tttatacatt ttatagttat gctctttcac attacgaata actacttcag
     3781 caatactact agatgtaact ataataagtg atttgttccc ctcatttttc attttatgtt
     3841 tcaagtaatg tttccataag aggcgagtta tatatgtaat tacaacgtat aaacctccca
     3901 tcaagaaaag aacgactcta gaataatcat ttccaccctg aacactaatg agatacaaac
     3961 ttcctaacag caccaacata atcgcttgct ttacagaaga agcaaattct atatagtacc
     4021 ctctttttaa tacacctcta tatgcttcaa acatgaaaat aactaaaaga tctgccagtt
     4081 caatgaagac tgccatgttt tgatacattg gaatcacata aggattccaa tctacctggc
     4141 gtaagacata cgatagataa aaagcaagct ggatacacaa taaatctaaa ataataaaat
     4201 caatatgttt aacccatccc cttgaacttt tcttgtacat tcctttttac tcccctcttt
     4261 ttaataaaat aaattgtatt acttgttttc tctcctaaat atctttctga aggattattg
     4321 actcgacaaa ttggcttgtg gatattctta tatatagaac atccgtaagc caacttaaaa
     4381 ctccttaaca aaattttttt actcatattg tagatcaaaa caagtatcca agaaatcata
     4441 ttttatttct tttttctgct tactcaacaa cagctaaaag aaaaatcatc ataaacaaat
     4501 tcaactaagc caacgatccc cttgtgaaat caagtttttt cttgatttac gaataaagag
     4561 ctaaaataaa ccaaacttct tcttttttac ctcttcatag actggataac tgatttcatc
     4621 cccgttgatc agatcctttg cgacttgttt catctgtact actttttcct taccaaagtc
     4681 ctttgcaatc tgctcatagc actcttttaa gtaaaaggtg cgcttagaga ccccatgggc
     4741 atcagaagca gccatctgca cgagattatg tttgaccatc gttttggctg ttttctggat
     4801 gtctttgcca aataccccaa cataacttgg cgcagtgagt tgggccaaac atcccatatc
     4861 taggaacgga agcaaatgat ttgggtcttc gcgaaaatgc acgttgcgtt ctggatggac
     4921 gattactggt gtctttccta gttctaacaa ctcaaaaaaa agccgttctg catataacgg
     4981 cacttccagt gtcggaaatt cgatcagaag atatgtatca tctaaatctg taaataatat
     5041 ttcatctcgg tggatgtctt cgatgagcgt tccggtaata cgtacttctt gtccttccaa
     5101 taacgtcaaa ggcaactgtc ttttctctaa ttcggcttgt aacgaagcga ctagagaaat
     5161 gacttgtgat ttttcattgc tgtattttcc attattgtgg tgtggtgtgc ataaaatatg
     5221 cgtgatgcct tgctgaatcg ctttttcagc cattgcaata cttgcttcta agttttctgc
     5281 cccgtcatcg attcctggta ggatatggca atgtagatcg atcatttctc ttgtctcctc
     5341 tcttcttatt ccttaatttc cgtagtaata accagcatct ttgctatgtt ctgctccgtt
     5401 gtacacaaca cctaagactc gtgcttgtac catctccagc agttcttttg ctttagtcaa
     5461 ggattcttta cgtgacatat tttcacgtac gaccaataaa gtgccatccg tttttaaaga
     5521 catgatctgt gcatctgtta cggctactac tggtggcata tcgaatatca ccacgtcaaa
     5581 taactgtctt gcttcggata ggatctgatc cattcttggc gagcttaaca attctgatgg
     5641 gttgggtggc ttaggtccac taggcaaaat cgacaagttc tcgactggcg ttcgttggat
     5701 cacatctgct acacttcctg atgaactaag tgctgtactt aatccactgg cattgttcag
     5761 ctggaatgtc ttatagacga cgggtttacg taaatctgca tcgactagta gaactcgttg
     5821 acctgattta gcaaaaacga ctgcaatgtt tgctgcagtg gtggatttcc cttctccagg
     5881 acctgatgaa gtcacgacaa tcgttttgat ttgctgacca gctgcggagg caaactggat
     5941 attcgtccgg atcgtacgat attgttcggc gattggtgaa gatgggtttg ttaatgtgat
     6001 caaactgact gcatgagttt gtgctgcttt ttgtttctgt gttcgtgcca tttttcttcc
     6061 tcctagactc ttgagcggct tctgcgtgat ggggctgttt tatcgtttgt agcttttggt
     6121 cgtgcaggag ctgttccttt tttacggata ccagcgttca attctttcgc tgacatttct
     6181 ggaactgttc ctaagattgg gaatcctaat gtatctgtca catatttttc atctttcact
     6241 gtccgatcga gtaattccaa taagaatgcc agacctacac ctaccatcat tcctaacatt
     6301 aagccagtca ttaaattcaa tttattattt ggcgaaacag gtgtcgtatt ggcaactgca
     6361 cctgaaatga tcgagatttt atcaacattc atcacatctt ttgcgttctc ttggaaaact
     6421 tgtgaagttg tgttagcgat ctgttgagca gccactgcat tcgtacttat tgcctgaatc
     6481 gagaacattt gtgaattttc tgcctgatta actgagatgg catctttgat ttgttcagca
     6541 gtcattttca cattgtcttc tgtttctaaa cgatctttga cttcattgag cactaaatca
     6601 ctgacgatca tatctttata cgtattgatc atttgcaggt tcgtattgac atcattgaca
     6661 tttgcagtgt ctgattgtgg cagtgtcacg atcaattgtg cttgtgaact gtattttggt
     6721 gtcaagatga aaaatgttac gattcctgag atggccaagc cagcaaacat tgagatgatg
     6781 attattacca agtgtttgcg taatgtttca aaaatttctt tcaagctgat tgtctcttcc
     6841 attttttctc cactttccta tttattctct agattcattt gatcttttaa ttcttttttc
     6901 acacgatcaa gttcttgatc actgactttt tgataggaga tattatcttg catgaagcct
     6961 tctccttgca gctgatcttg cttaatcgta ccaaatgcct cccgataatc taaagcaatt
     7021 tttttcattt catcaaagtt catgtctgtt ttcatattcg actctagagc attcaaaacg
     7081 gattgatagt tgctgacacc gtttagactc aatacttttt tcgctacgcc ttctacgatc
     7141 tttctttggc gcccttgtct accgtaatca ccatttgggt cttcataacg catacgagaa
     7201 taagataatg cctgatcccc gttcaatgta attttaccga ttggaaagtc atagccatct
     7261 tgtgagaaag tgaggtcatt attgacttcg ataccaccaa ctgcatcaac gagttccttt
     7321 agtcctttca tattgataga gacatagtga tcgattggga tgtctacata tttttgtacg
     7381 gtatccatcg ccatggcagc tccgccgaat gcgtaagcgt ggttgatctt gtcttttgtg
     7441 tcacgaccta cgatgtcgac atacgtatct cgaggaatgc tgacgatcgt tgtctgtttg
     7501 tcttcaggac tgactgtggc aaccatcatc gtatctgatc ttccttgatc gacccgacct
     7561 aaatctccag tatcaatccc catcaataga acagagaaag attcttgttt gtccagatca
     7621 actggtttcg ttcgttctcc ttgggttcgt tccactgatt cgtaagtttt ctcaatagaa
     7681 tgtgagagat ccagatataa ttttgctcct actcctacaa ttgctaatac taatacgata
     7741 agaactgcta aaataatcaa aaatattttt ttccctcttg attttcttct tcgtcttgcc
     7801 atagtttcct ccgtttgcta accttgcttg aatcaacgtt ttaactgtca atagcttata
     7861 ttgctatagc ccatttgctg agcaacagtt tgtgcttttg ttgcgtatgc ggctcttatt
     7921 ttttcattcc ggatctgctc tatttgactg agcgccgtca aatattgatc gtaagtagcg
     7981 gtagctgtta cttgaccgtc tttcagcatc gtatcgaata gctgatctag ttgcgtgact
     8041 tgtgcaattt gggtatcagc gaatcctaga tattgcataa cgactgtttt ccaagcacta
     8101 tccttaaaaa agctcatatt ttcacgtaca tgggcgacat ctgcacttgc caacttttct
     8161 ttgatgatga catcatccac agcttgctgc cagtttgata cgtttttagt aaataattta
     8221 ttgacagccg tctggatctt cagttttgat tctgcatcct ctaactgcgt taagactgct
     8281 ttcttttctt cttggatcgc agcagcttct ttaggtaatt ctgattcctt gatgtcataa
     8341 tcatctgccg aaagtttgat agaagatagt ttatgtgaca aatttgaaac tgtttcttct
     8401 gttatttcag gatttaggaa aattttttct tttgtactgt acagcttatc tacttctttt
     8461 gataaagagg tcacggtgtc tttttgattg gtgatggcag attctgcgta attgatccgt
     8521 tgatgatatt gccgctgatt attgagatgg tatccaaaca agcaaatcac aatagcgcct
     8581 gtgcctaccg ccacagtagc caataacttg acgaacggct tttccataga ttcactccta
     8641 aatattgatg atagataagt aaagatgtga ttcattcaca tcagaatgag cgctttttat
     8701 tttaaaagca taattaaact aatata
//
LOCUS       DAYYBH010000098         8678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000098 DAYYBH010000000
VERSION     DAYYBH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.101"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            203..403
                     /locus_tag="ACHAUG_002557"
                     /pseudo
     CDS             203..403
                     /locus_tag="ACHAUG_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1115..1720)
                     /locus_tag="ACHAUG_002558"
     CDS             complement(1115..1720)
                     /locus_tag="ACHAUG_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8481608.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            1902..2237
                     /locus_tag="ACHAUG_002559"
                     /pseudo
     CDS             1902..2237
                     /locus_tag="ACHAUG_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2402..2617
                     /locus_tag="ACHAUG_002560"
                     /pseudo
     CDS             2402..2617
                     /locus_tag="ACHAUG_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2755..3726
                     /locus_tag="ACHAUG_002561"
     CDS             2755..3726
                     /locus_tag="ACHAUG_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8481609.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            3866..5179
                     /locus_tag="ACHAUG_002562"
     CDS             3866..5179
                     /locus_tag="ACHAUG_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFM8481610.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5222..6088
                     /locus_tag="ACHAUG_002563"
     CDS             5222..6088
                     /locus_tag="ACHAUG_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8481611.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6085..6933
                     /locus_tag="ACHAUG_002564"
     CDS             6085..6933
                     /locus_tag="ACHAUG_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8481612.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            6947..8419
                     /locus_tag="ACHAUG_002565"
     CDS             6947..8419
                     /locus_tag="ACHAUG_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8481613.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8436..>8678
                     /locus_tag="ACHAUG_002566"
     CDS             8436..>8678
                     /locus_tag="ACHAUG_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8481614.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2736 a         1489 c         1784 g         2669 t
ORIGIN      
        1 agaacaccac gcatagtttt tattttgaga tattctagaa tgaaacacat taagaacgaa
       61 agcaaagcaa aagacgccag aacttctact accttctaag ttttttcata tttgtttgtt
      121 atgctataca tagatctgca taattagact tgcgctgccc gttattgaag tttcaggcag
      181 cgctcttttt gggggatcga taatggaaga agaaaaaata tttgagaaac gctggcagct
      241 agcatcaagt gaacaaagag caagatacaa taatttgatc acctcctacc ccactgttga
      301 ctagacatat aaggaaaaga aatacttact ttggctgtgc cagttagata tagatacctt
      361 tgaaacattt gaagtgatcc tagataaaat taaacagaat tgatcagaaa caagcgaagc
      421 cttctctcgg ttcgcttgtt ttgctattta ttagttggct cagggcaact ctaagctcgt
      481 ttgaaagaaa accgatggat aatccccttg tttgtaccac tttagaatac atggatgtac
      541 gtattgccat tgactcctat tagttactga aaaaaaattt aaacaagttg agtattgcag
      601 ataaatagct ccagtgaggt gaataactgc tccactcaag gcaatagata gctccatcca
      661 gggcaacaaa ctgctccact ttcatattta atagatgaat aaccttctgt ataatagaaa
      721 ggcatacctt tcggtatgac aacttataca gaatgttatt ttattggttc actatcgaaa
      781 aattttggag ctttattttt ccggtatttc tcaacgaacg attagtttaa ctgtgggaag
      841 ctccagacat acgattcgag aagttacttc tcgagcagac aggaaggggg tgactgaact
      901 aaccgctgat atgagcgata cttggctgta ggaatatctt tttccggaaa aacaaacaac
      961 agcacgaggt taccttcctg tagactggga atatgttcat aaagaactaa tgaaaaagaa
     1021 tatgacattg aaactccttc atagagaata tggggtacat ggaacataga aacgaaaatg
     1081 cacggtagaa aagcgaatga atatagtata aattctacaa atcattcgct ttctttattt
     1141 tatatatggt tggtcgagag agaccatttt ctttggcaat ttctgcaact ggaactcctt
     1201 gatttaacat gtccaccaca gttttaaaga ctaatcgttt ttgcggatct gttgagttct
     1261 cagaatattc aattggtcgc cccttatatc gaccagcttt tttggccaat aatactcctt
     1321 gccgctgacg ttcacggatt ttctcacgtt cgttttgtgc aatataactt agcaatgata
     1381 aaaacatatc gaacatagca gtatctagga gttcatttcc agtattaaaa tctaaaaact
     1441 gtgcatctag tatttttaat gctacttttt tctgtttcat aaaggtaact gtatttttta
     1501 tgtcttcata atctctgcct aggcgatcca gtgatgcaac aatgacacaa tcaccttctc
     1561 ggatataatt tagtaatttt tgaaactcag gtcgctggat atctttacca ctggcttttt
     1621 cttggaataa tttatcaatg ttaaggtctt ccatcattgc caattgtcga tctaaatttt
     1681 gatcttgtga agacactcta atatatccaa tttttgccat aaaaactcct tttaaaaaat
     1741 gatataattt acatgtaaaa tttacttgta gttttactgt tatcatagca tttttaaaaa
     1801 taaattttaa gtctacttat aatttacttt ggaggttttg aatgaatgaa attaagcgca
     1861 ataaaaattc ttttggtgtt tatttaggag agcctagctc ggaacagtta aataaaggtg
     1921 aagctcgtca tgctctatgt cgaaatatat tttatggtag aaaaggcaaa ctttatcaaa
     1981 cttattacga cggtatggag gaacagctca acgcattagg attagtaact aatgctatca
     2041 tatattggaa ttcactatat ttagaaaaag tctttgaaca aattaaaacg gaagggttcg
     2101 actgttccga agaagtgtta cgcaagcaat ttccattaat tagtgatcac attaattttg
     2161 ttggcaaata tagtttccgc tatgaaaaag aattatcgaa tggaatacta cgtccgttaa
     2221 atatagagaa ttattgagaa tgttcgtctt ttggttccta ccgtgcattt tcgcacggat
     2281 gttccagata ctatttttga atgtataaaa ttgaaaacac aagaatacta ataaatcaac
     2341 gattcttgtg tttttaattt ttcactaccg tgcattttcg tttctatgtt ccatgtaccc
     2401 caatcttgaa aaattaatta aagacttaga taaagccatt gacgacttag tcgtggtgct
     2461 accggagtat caatgtttaa ccagtatccc aggtattggt cctgtttagg ctgctggaat
     2521 actcgctgaa attggtccaa ttgaacgttt ttctgatgaa ataaaattga caaagtatgc
     2581 cggcctatat tggcgacaga gtcaatccgg taaatctgaa tacgaaaatt tttttacgaa
     2641 aagcttcacg tctaatatct attttaaaat aaaaatattc ctgctaattt ggaatcgttt
     2701 ccttcttttg gtataataat acctgaaatt cttttgagag gagttgttcg tgaagtgaaa
     2761 actactatga aggatgttgc tgcgttagca ggagttggtg tgggtacggt atctagagta
     2821 ataaatggag taaaggtgaa agaggttacc aaagaaaaag taatggctgc aattagtgag
     2881 cttaattatg agcctgatga atatgccaga ggtatgaaaa cgaatagtag caatacaata
     2941 gctcttattt tacctacgat ttggcatccc ttcttttccg agtttgccta ctatgtagag
     3001 gaagctctca gccaaaaaaa ttataagctt tttttgtgta atgcgaacgg cagtgcggaa
     3061 aaagaaaaag aatatatcca aatggtcaag cagagtaaag tagacggaat tataggtatt
     3121 acctattcag acattgataa atatgtatca tcaaatttac catttgtcag tatagaccgc
     3181 catttttcgg aacaggtttc ttatgtaacg gcagataatt atttaggtgg gcaaattgcc
     3241 gctcaagaac tacttactcg taactgtaaa aacttgtgct atgttggggg tgtttcacct
     3301 taccctaacg aaacaaacaa tcgtaaagct ggtttttaca atatttgtga ggaaaataaa
     3361 atagctgtga aggtattgga tatgcctgaa ccaattagca atttagagga acaattgctc
     3421 gctttttttt cggataatcc tgaaattgat agcattttta ccatcaatga tttcatggct
     3481 ttggatgtga taaaagccca aaaaaaacta gggaaggaag cacctaagga ttttcaaatt
     3541 attggttttg atggagtcaa gctatcgttg caaaaagact atttactatc aacgattgta
     3601 cagccgatag aggaaatggc caaggtttca gtagaaacac tcatggcgat gattttaaat
     3661 gaagaaccgc ctaagcgtgt ggtagtaccg gttttctttc acgctggaga gacgactaaa
     3721 gagtaaaaaa ccggtttata acgatttttt tgagataggt tgacagaaag tgatttcaat
     3781 gatatcttaa aaccaattaa ttggaaacgt taccaattaa ttgaaaaata aaacggaaac
     3841 gtttcaaaaa ataggaggaa catcgatgaa aaagagaatg ttagcaattg cagcaatttt
     3901 agcattaaca gctggaggac ttgcaggatg tggcagtaag gagaaaagtt caggtagcag
     3961 tgatccagga gaaattaaga tttgggttca gttttctgat gaaacgcctg aaggaaaagc
     4021 ttgggcagag attgtgaaca attttaatga tgaatacgag ggtgaatata aagctgttac
     4081 tgagtatatt cctcgtagcg gaagcggcgg tggatatgag gataaagtaa atgctgcaat
     4141 tacaaccaat acccttcctg atgttattac gttagatgga ccgaatactg cagcttatgc
     4201 tcattcagga gtcattgctc cattagatga ttatttgaaa gatgcggaca tggatgatgt
     4261 attagacagc attattcagc aaggaaccta cgatggcaaa atgtatgcgt ttggtttctc
     4321 agagtccagt gtgggtgtct attacaataa gtcgctgttc aaagatgctg gaattacaga
     4381 agatcaattg ccgacgttag aagatccttg gacttggacg gaatttcaag caatttgcca
     4441 aaagctgact gataaataca aaacgcctgc aattgatatg caaatggcgt cgggagacga
     4501 aatgctgacc tacgcatata caccatttat atggtcgaat ggcggcgatg tcgttaatgc
     4561 tgatggaacc aaagccgaag actattttaa cggtaaaaaa tccgttgaag cgttggaatt
     4621 tattcaagaa ttggttgaaa aaggttacac cacaatcagt cctattgaaa aaggatttga
     4681 aactggcaaa tatgccatga tgctgagcgg ttcttggaca atggcagatt tagaggaaaa
     4741 ctatcaagag attgattatg gtattttgcc ttaccccgtt tcagatgcaa ctggggagtt
     4801 agtttcacca accggaagct ggcagttagc aatgactgag aaaacagaga aaaaggatgc
     4861 ttctgcggcc tttattaagt ttgcaaccag tacagaatct agtcgtatta tgagtttagg
     4921 gaacagtgtt ctacctatca gaaattcaac agttgattta attaaagatg aagtgtcgga
     4981 aggaatgcag gtattaatta agcaaaacca actttctgga catgcccgcc cagtggttgt
     5041 agcttatccg caagtatcta gagcattcca acaagcagtt caagatatta gctacttcga
     5101 ggaaaaccca gatgtacaaa aagtagcaga tagtcgcgct gctgaaatgc aacaggcaat
     5161 tgatacttca ctaaaataga agtttctaaa gggggccaat caagtgttaa aaaaaccgac
     5221 aatgaaagac aatttgatag gttacggctt tctagcgcca gctttgctgc tgctgacgat
     5281 ttttctgatc atacccgtaa tcatggtaat ttattatgcc tttactgatt attatttgtt
     5341 aacgccagat gctagacaat ttatcggctt agaaaatttt aagcgactgt tcgctgatcc
     5401 aatttttgta aaaagtattt ggaacacggc taaatttgtt gtttggatta ttccggtcca
     5461 aataggggca gctttaggaa tggcacttat tatcaacaaa aatcgtaaag gaaatatctt
     5521 ttttaaagta gccttttttg cgccagtcgt gatgtcctta gtcgttattt cgattttatg
     5581 gttgtacttg ttaaatccta atgaaggtct ggttaatacg ctatttgcaa agattggaat
     5641 tgcttcgcag ccttttttga ctagtcccaa acaagcaatg tatacgcttg ttttcgtatc
     5701 ggcatggcaa ggtgcaggat atcagatgct gctgttttta gggggaatgc aaaatattcc
     5761 gcaagacgtt tatgaggcag ccgaaattga tggtttttct aagtttcagc aatttcgcta
     5821 tatcaccatg ccattattaa agcccacggc gatttttatt ttacttacca cattgatttc
     5881 ggcctttaaa ctaatcattc agccaatggt aatgactcaa ggtggaccaa tgaattctac
     5941 tatgacaatg gtttattaca tttaccaaac tggatttaca gaccgtatgg taggctactc
     6001 cagctccatt gcgttaatct ttaccactat gattggttta attacaattg ctcaaagaaa
     6061 attggtaaag gaggatgatc cagcatgacc aagaaatttg gaccgctaat gatcgtagaa
     6121 tattgtttgc tgttcctttt agcggcatta ttcattttcc cgatgctgtg gatggtagtt
     6181 tcctctatga agcctgaagc agatgtctat caaaatttag cttccttcaa agctttttta
     6241 cctagtttga atccagctaa ttggtttaaa acttatcaag aggttatttc ccggttcagt
     6301 gtgggaactt atttattaaa cagtattttt tatggtttaa cctttgcctg tgggtcaatc
     6361 attgtaaact ctttggcggg ttttgccttt gcgaaggtta atttttcagg caagaaaatt
     6421 atctttggag ctttgttagc tctgctgatt gtcccgatgg agacggtttt aattccgcaa
     6481 tttaccatcg ttaattcact aggcttagtg aatactcgct tggcagtgat tttaccagcg
     6541 atggccagcg cgtttaatat ctatctgttt agaaatttct ttatcgcgat tccagaagaa
     6601 atcattgaat cagcgaaatt agatggtgca aatattgtta aaattttctt tcacatcatg
     6661 ctgccaatgt ctaaaccagc catagccaca gtaggtgtct tatcctttat ctccagctgg
     6721 aatgattata tttggccgtt aatggtctta actgacagca gcaaattttc aatgcaagtg
     6781 gcaattacta cgatcaatac tacacagcca gtttatatca atcaagtgat ggctgtttta
     6841 accattgcaa cgattccact gattattgtt tatatcgttg cacaaaaata tattctgcag
     6901 ggacttggcg gttctggaac gggtataaaa taggagtgaa tagtgaatgt tgcaaaaact
     6961 tttaaaggcc aatgagttta ttgagaaaaa tgctcaaaca gtcaatcaag aataccggaa
     7021 taaatttcat ttgatggctc cgattggctg gatgaatgat ccaaatggtt ttgtttatta
     7081 tcaagaggag tatcacttgt tttatcaata ttatccttac gacagcatct ggggaccaat
     7141 gcattggggc catgctaaaa gcaaggatct aattcactgg gagcatttgc cagtagcact
     7201 ggcaccaggt gaaagctatg acaaggaagg ctgtttttcc ggcagtgcca tagaaaagga
     7261 tggcaagctg tatttgattt atacaggaca caatgttgtg gaagggcaag ttcgacaagt
     7321 tcagtgtttg gcagtttcag aagatggcat tcactttgaa aaatatgccg gaaatccaat
     7381 catcgcagaa gatcacttag cttctgttgc aacgaccgaa gatttccgcg atcccaaagt
     7441 gtttcagcga aaagaagact attttactgt agtcgctact aaaactgcag atgctcgtgg
     7501 acaaattgtg atgttccatt ccaaagattt actaacttgg gagttttatt ccgtattgct
     7561 ggagggaact ccagaacaag gcattgtatg ggaatgccca gacttgtttg aactagatgg
     7621 caaagaagtc ttgattcttt ctcctattca aatgactcca caaggcaatg cttaccaaaa
     7681 tacgaactca actgttgctt tcatcggtac tgtcgattgg caaacaggca aatttctagt
     7741 agagaatttt catgaaatcg atggcggttt agatttttat gcaccccaga cctgtgaaaa
     7801 cgaagcaggc caaagaataa tgattgcttg gatgcaaatg tggcagcgta aaatacctac
     7861 gaatgatctg ggccatggtt ggtcaggttc catgaccttg cctagagagc tgagggtggc
     7921 tgataatcaa ttgttgcaac ggccagtttc atcaatttat caagtaattg atccgcagga
     7981 aaggattgaa tgggcgaaga ctgaagagaa tcgcttctta ctgaaggact tagtatatga
     8041 ccagcactat ttacgcttgg aaatcaattt aaaacaggca acgaaactag aacttcacta
     8101 tgcgaaggga cccgaccata cattactgct taactatgat gttgaacggg agtgctttac
     8161 tgcttccagg gaacacatgc cgtatgattt atcaggagct gaaaaggaag tgttgaaaaa
     8221 gcgcagtgtg acgattcctt tggaaaataa ttgtctaacc atcgaaattt tccgtgatac
     8281 ttcagctatt gagctttttc taaatgatcg tctgactatg agtacaactt tttatgagac
     8341 agaaaaagga caggattttc ttttgctggc agacagtgaa gtaacctttt cattggaaag
     8401 cggcttaatt aatctataga agggagagct cagtgatgat ttatggttca atcgaagcag
     8461 gcggcacaaa atttgtgtgt gccattagcg atgaagaaat gacgatcaag gaaagagtga
     8521 gctttccgac aacgacacct gaggaaacaa tacctttagt cattgacttc ttcaaacaat
     8581 accaagcaga tttagcaggg attgggattg gctcatttgg gcctatcgat atccatcgcg
     8641 attccgccac ttatgggtac attacctcga ccccgaaa
//
LOCUS       DAYYBH010000099         8642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000099 DAYYBH010000000
VERSION     DAYYBH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.102"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..2621)
                     /locus_tag="ACHAUG_002567"
     CDS             complement(240..2621)
                     /locus_tag="ACHAUG_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8481615.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2611..3258)
                     /gene="recU"
                     /locus_tag="ACHAUG_002568"
     CDS             complement(2611..3258)
                     /gene="recU"
                     /locus_tag="ACHAUG_002568"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8481616.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3322..3861
                     /locus_tag="ACHAUG_002569"
     CDS             3322..3861
                     /locus_tag="ACHAUG_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8481617.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3951..4370
                     /gene="gpsB"
                     /locus_tag="ACHAUG_002570"
     CDS             3951..4370
                     /gene="gpsB"
                     /locus_tag="ACHAUG_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8481618.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4392..4759
                     /gene="rnpB"
                     /locus_tag="ACHAUG_002571"
     ncRNA           4392..4759
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHAUG_002571"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4968..6134
                     /locus_tag="ACHAUG_002572"
     CDS             4968..6134
                     /locus_tag="ACHAUG_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8481619.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6180..7676
                     /locus_tag="ACHAUG_002573"
     CDS             6180..7676
                     /locus_tag="ACHAUG_002573"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8481620.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7757..8440
                     /locus_tag="ACHAUG_002574"
     CDS             7757..8440
                     /locus_tag="ACHAUG_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8481621.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8449..>8642
                     /locus_tag="ACHAUG_002575"
     CDS             8449..>8642
                     /locus_tag="ACHAUG_002575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481622.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2715 a         1569 c         1836 g         2522 t
ORIGIN      
        1 aaagactagt gtgtatttag ccgatttaat tataaacaag taaatggata aagtatatgt
       61 aatgataaaa gaaaaatgga ttttctaatt tttaagctaa atgttagatc acacatgtat
      121 atgctggata gtcttataaa aaatcatgta atacagcttg gtacatgatt tttatttgta
      181 gctgtttttt acatagacac aaaaaagaaa ccacgcttgt agacgtggtt tcttttttac
      241 tatctgtttc cagaagctga ggtgctgctg gatggtgtat ggtcatttgc agcatccgct
      301 ccgctagttg ctggctcact ggaagaagca ggaggagcag atgaagacgg tgtcgagctt
      361 tcttcggatg aagaagaagg aaccgttgtt gaagacgctg gctcgcttgt ttctgctgtc
      421 gtgctttctt ttgaggactc agcagttgat tcgctttgac tagtagagct agagctttga
      481 gtcgttgtct ctgttgttga gcttccagga gtctggacat agctggaaga tggaatagtt
      541 gttgatggtg taattgcatt cgatctagct gtatattgat ctttgtagta cagttcaccg
      601 cccacacgaa ttaatccgct tggcatctcc cagtctgtat ttgtcacgtt agctgaaaca
      661 tactgcataa gctcgcgata aacatcagag gctacgtgtg acgattctga agtgacaggc
      721 gtcatttttt tattgtaacc tgtccacacg gaaatcgaat aatttggcgt atagcccgca
      781 aataggatat ctggatacac cccagaagaa ataccaagtt ttgcatactc gtcatctgtg
      841 taattggatg ttccggtctt accggcttga tataacccag cgatttgcgc attcgtacca
      901 gttccttcag tgatcgtatc ttttaaaatg tctgtgatca tataagctgt ttctggagac
      961 atggctgttt ttccatctgg ctcataagtt tcttctgttc cgtcttggaa gacgatttta
     1021 ttaacatatt gaggtttgta ataagtccct ccattggcga aagccgcata ggctgctgcc
     1081 atttttaagg aagaagctcc gtattttgtc ccatcttgtt cttccgtatt actagagatc
     1141 gcattcgatt gatggatcgt actgtattcg atcccaagat tttttaagaa agaagccact
     1201 ttatctgaac cgacttcatt gaataatttg actgctggaa cattacggga taagtaaagt
     1261 gcttggcgta aagtaatggt gcccatatat tgattatccc agtttcctac aggagtcgaa
     1321 gtcccttcat agttatacgg tgcgtcagta atagtttttc cagtagaata tttcaaatat
     1381 tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag aaccaaaatc acgggaagta
     1441 ttcactgcta agttgttgcc taaagttacg tcttctgcga tgtgacgtcc gccaatctgg
     1501 gcttttactt tcccggtatt ggtgtcgata agggtggagg caacttgcat ctcgtcgtct
     1561 gggtaagaga catactgatc cgtgttgacg atatcatata gtttcttttg ggcgtccaga
     1621 tccaaattcg tataaatctc taaaccgtct gtgtagacat tcttatctgt tttttcttgt
     1681 acttcattga tgacttcttt gacataatta tcgacgatct tagtattgtc atcggattga
     1741 gttagttctt gcagaccatc cgttaccggt acattaacag cttgatcata ttctgtttga
     1801 gagatttttt cgttttgaag catcgtatac aaaacagtat ctcgtctttt cttcgcctga
     1861 tctggataaa cataaggatc ataggcggaa ggggcttgag gcatcccagc aagcaatgct
     1921 gtttgcggta aagaaagttc tgataatttc ttaccaaagt acatctcaga agctgtctcc
     1981 atcccgtaca agccgtttga catatacact ttatttacgt agtaggtcaa gatctcttgt
     2041 ttcgattttt tttgttccaa tcttacagcc atccacgctt cttgggcctt gcgtttcaga
     2101 gtctgatcct cggcactggt ggagaagaag gaaagtttga tcaattgctg tgtcaaagta
     2161 cttccgcctt gcagtccgcc agaggtaaag ttagagaggg cagaaccgat gatccgaata
     2221 gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta ccgacacgat cgcatcttct
     2281 aatgttttcg gtaattcatt cgcagaaatt ttttcacgct tttctgcgcc taaatcttcg
     2341 aatagttcgc catcttgtgt atatagtttg gaagatacgg tagcgtctaa ttttttatcc
     2401 gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc ctacacctgc taaaaagagg
     2461 atacagaaaa atgagagtat tcctaaaagg atcttgataa ataatccttt tttatgtgtt
     2521 ccagaggact ttccagaacc tttgccagaa ttcttcttca cactcttttt aggagtaggc
     2581 tgtttctgtc gtctagagct tctagtttgt tcatttgcca ttcttgcttt ctccttcaat
     2641 agataatgtt gataaataga agtcaaccgc gtccaaataa ggaatccgcg gagaaatacc
     2701 agttttgatc tctattccta gttcttcgat caaagataaa ggtagtgatt tgcgtccttt
     2761 ttccttttgg gcgtcccagt aagcaatcaa atgaacacta ttcaaataaa aacagcgctt
     2821 caaagacgaa aaccataaca agacaaagca gaccccattt tgttcgaggc atgctttcat
     2881 atgttcgatt tgatgggcat ggaagttttt aaagggaaaa gatgttttgt tcttggtttc
     2941 ttttgcttca aagtccaagt aaaagcctcg atagacgcca ttatagtctg tagttgaggc
     3001 ttgggtaaag taggcttcct tgatcacagc agcacttctc ttaggatagt ctactttcac
     3061 gatttggata ggggtaggct ttttatggat aaccgccaac ccgcgggaaa gataatattt
     3121 attactttca ttgattgctt cttcaaagcg catcccccga ttgccgaatt cagtgatctg
     3181 atttcttttt tcttgaattt tttcgacggg agaggcattc ttataataag gttggccatt
     3241 tggatagcgc aataccatct ttaatcacac tcctaggcca cattatagca aaaaagcgga
     3301 tagaaagaaa aggaatctga gatggataag tcaaaagtaa tctatgtgtc tgggtatcga
     3361 agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa tccaagttgt taaaaatgtt
     3421 ttaaaaaaag aactccatca gttagcagaa gaagggctgg aatgggtact tgtcagtggg
     3481 aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg aattgaaaga agaatatccg
     3541 gaactatctc ttggtatttt gtatcctttt tctgattttg gtaataactg gaatgaaggg
     3601 aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt atgtcgaagc tgttagccat
     3661 caaccctacc aatcacctag tcagctaaag aatcacactc gttttatact tgagcataca
     3721 gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa aaacgaagta tttttttgaa
     3781 gatgcacaga attaccaagc agatcatccc tacgaaattc ggctgatcag tatggacgat
     3841 cttcaaaatt ttagtgaata atcgatattg tgtttgattt atcaactttt ttcctataca
     3901 atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag aggtgtaaat atggcgaatt
     3961 tggtttatag tcctaaagac attttacaac aagaattcaa aacaaaaatg cgcggctatg
     4021 atcccgttga agttgatgaa ttcttagaca atatcatcaa agattatgaa acttacagca
     4081 aagaactcct tgctttacaa gaagaaaacg atcgattgag tgcaaaagta gctcaattat
     4141 ctaaaactca aggagcagct caaacacgcg tgcaacaaac agaggcacca aagagcgcag
     4201 cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct tgaacgtgaa gtatttggta
     4261 aaaagcttga tcaacaagca agtgcagtca aaccagcaca acctaatcca aataactaca
     4321 caaatgcaga cacaagctta gatgataatg aaaaaacacg tcaattttaa tttttgatta
     4381 atgaatttgc ggttttcggg taatcgcggt ctgacttgat caggctgagg aaagtccatg
     4441 ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg aaaccataag ctaaggtact
     4501 ctttggagta acggcaggaa aacaagctaa ggcttcggct atgcttaagt atccttgaaa
     4561 gtgccacagt gacgaagcgg ctggggaaac ccatccggtg gaacgcggta aacccctcga
     4621 gcgagcaacc caaacaatgg taggggcgct ttttgtctgg aagaagaaca ggacaaaaag
     4681 gcagtataac tgcagataga tggttaccgc tttgtttttt ctccctggaa aagcaaagta
     4741 cagaacatgg cttatagaaa accgcaaaca tcagggaatt aggtcgtgac aaaagtaatg
     4801 cgttataaga aacaagaaaa acatttgaaa gctatttttc aatacttcag atgaatcgtt
     4861 cttattacaa aacaaacttt tgtatcaccg gttattcgga atttgagtaa aagtgtgggg
     4921 taggactgat gtcccacctc ctttttttat aaataaggag attgtgtatg gaaaaaaaca
     4981 ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga agcactggtt ggtaaagaat
     5041 taagagatct tggaatcgaa tgccaagttg agaacggacg tgcacgtttt gaaggaacaa
     5101 tggaaacgat cgctacagct aatttatggt tacgtacagc agatcgagta aaaatcgtgg
     5161 ttggggaatt cgatgcgtat acgtttgatg aattgtttga aaacgtaaaa gcattgcctt
     5221 gggaagattt tcttccacta gatgcagctt ttcctgtagc tggaaaatcg atcaagtcga
     5281 aactatatag cacacctgat tgccaagcga tcacaaaaaa agcaatcgtt gaacgtctcc
     5341 gtacctatta tcaccgtcca gcttctgtac cactgacgga aactggcgca cattttcagt
     5401 tggaagtagc tttgctgaaa gaccatgtga tggtaactct ggatacgaca ggaccaagtt
     5461 tattcaaacg tggttatcgc ttagaaaaag gtggagcacc attgaaagaa aacatggcag
     5521 cagcacttat tatgttgaca aattggcgaa aagaccgtcc attttatgat cctgtatgtg
     5581 gttcaggtac gctttgtatt gaagcagcct taataggcca taatatcgca ccaggattca
     5641 atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca agaaatcatg gaaaatgttc
     5701 gatcagcagc cgaagaaaaa gcagattatg atattgaatt agatattact ggttcagata
     5761 tcaatggacg gatgattgaa attgccaaag ccaatgcaga agaaatcgga ttaggctctt
     5821 cgattacctt taagcaacaa gcagtcaaag acttcaaaac ggataaagaa tacggggtca
     5881 tcgtggctaa tccgccttac ggagaacgtc taggagaaga agaaactgtt cggaaattat
     5941 ataaagaaat gggcgatgta ttccgtccat taaaaacatg gagtaaatat atcttgacta
     6001 gcgacttagc gtttgaggaa ttttacggac aaaaagcaac caaaaaaaga aaattgtaca
     6061 atggtgcatt gcgtacggat ctattccagt attggggaac tcgtccgcct agaaaaaaag
     6121 agaaagacga ataatcttac taattgattc attcaattgg atgtttggag gaatagggaa
     6181 tgaaagaaaa agagttttta caagaagtaa aagaaatcaa tcttctacaa caagctttag
     6241 gaatcctcga ttgggataca caaacaggta tgcctgaaaa agctagtgca taccgaagtg
     6301 aagtagacag ctatctttac agcatttatt tttcaaaaaa aatcggtcca aaaatcaaag
     6361 aagccattcg ctatttttct gaacatccgg aagaattgtc ggaagttgga actgccgttt
     6421 ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt accggaagat cttatgagaa
     6481 aatatacagc tgccgtttct tcagcacacg gacaatggca aaaagctaga gaaacacaac
     6541 aattttctga ttttcaacac gcattgaatc aaaatattga attaacaaaa cagttgatac
     6601 cttactggag aaaagatgaa aagacgaatt atgatgtttt gctgaatcaa tacgaacccg
     6661 ggatgactgt cgaaatcctt gataaggtat ttgatcagct taaagaagga attctttcga
     6721 tccgccgtac gctccaagaa aaagggactg aacctgaaac agacttttta aatcggagaa
     6781 tgacgaaaaa acaacagaag agatttgtag tgaaagtaat cgaacaactt ggttatgatt
     6841 ttagtcgtgg tcgtttagat gacactgtcc acccatttat gactggaatt aatccgaatg
     6901 atgcacgaat cacgacacga tggaacgaaa cagatttcaa aatggctgtt tttggagtga
     6961 tccacgaggc tggtcatggt atgtatgaac aagacatcga tgataaatat gcgtatacac
     7021 cggtttatga aggtacatca atgggtatcc atgaatcaca atcgttgttt aacgagatca
     7081 ttattggaag tagtcgcagc ttctggaaaa agcaatatcc tttctttcaa gaatgtgcag
     7141 aaggaacatt cgatgatatt ccttttgaca aattttatcg ttcattgaac catacaaaat
     7201 caagtttggt tcgtatcgag gcagacagtt tgacttaccc tctccatatc atcattcgtt
     7261 atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt tgctgaacta cctgaaatat
     7321 ggaatcaaaa atatgaagag tatctgggaa tacgaccaac aaatgatgtg gaaggcatcc
     7381 tacaagacgt tcattggtca ggtgcaagct ttggttattt tccctcttat gcattagggt
     7441 acatgtatgc ggcacaatta cttcattctt tgcagcaaga aatagatgtg gaaaatgttc
     7501 tagcgtcaga cgattattct ccaatcaaaa actggatgtc agagcatatc catcaatatg
     7561 gtgcatctag aaaaccaaat caattaatca tggatgcaac acatgaaccg cttaatccgc
     7621 aatacctgat cgactatatg aaagacctgt attttaatgt atatcaggta aaataaaata
     7681 caatttacaa agatagctga aattcatcta atctatagat gttttcagct atttacttaa
     7741 gaaagaagtg gaacgaatgg ataaattaca agtgatcgct gattatagta tggaccaatt
     7801 agcgcatgat caaacaggac atgggagcga tcataccaaa agagttgtca agttagctga
     7861 aaaaattttg gagacggaac cgcaggcaga ccgctttgtg acgcttgcag cagcttacct
     7921 acatgatacg attgatgata aagtcgttaa aaatgagaac gaggcaaagc aacagcttcg
     7981 tgtattcctt cgctctttgc caatagtaga ggaacagatc tcgatgattt tttccattat
     8041 cgaaaatatg tccttttcta aaaatctctc tgaagcggcc gaactatctc tggaaggtaa
     8101 gatcgttcaa gacgctgatc gtatcgaagc attaggagca atcggcattt tgcgtactgc
     8161 ttatttcggc ggtggacatg ggcacccgat ttttgatcct gaactgtatc cgcaaacatt
     8221 caaggacaaa gaaaattacc gaaaaggcac gacagtgatc aatcattttt atgaaaaact
     8281 gttcttgctg tctgataaaa tgaatactga ttacggatat gaagaagcca aacgaagaga
     8341 aaccttcatg cgtgaattct tagaagaatt ttttctggaa tggtctattg acgatgaaaa
     8401 ttttactcca gacaattgga tcaaaaataa caaaaaatga tggaggttgt gacagaagtg
     8461 gtcagctcca agatatttcg gatcattttc cgtaaaagtt acttctggaa cactgtttac
     8521 caagaggtcg tgagcctgcc gttcagctcc gagtcacaag gaacaatttc aaaaaatagc
     8581 ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat tggcaggaga acaccgttta
     8641 tt
//
LOCUS       DAYYBH010000100         8586 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000100 DAYYBH010000000
VERSION     DAYYBH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.103"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1511)
                     /locus_tag="ACHAUG_002576"
     CDS             complement(45..1511)
                     /locus_tag="ACHAUG_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8481623.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="ACHAUG_002577"
     CDS             1898..2494
                     /locus_tag="ACHAUG_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8481624.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="ACHAUG_002578"
     CDS             complement(2552..3034)
                     /locus_tag="ACHAUG_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481625.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="ACHAUG_002579"
     CDS             complement(3140..3337)
                     /locus_tag="ACHAUG_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481626.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="ACHAUG_002580"
     CDS             3632..4390
                     /locus_tag="ACHAUG_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8481627.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="ACHAUG_002581"
     CDS             4450..4878
                     /locus_tag="ACHAUG_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481628.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="ACHAUG_002582"
     tRNA            4924..4996
                     /locus_tag="ACHAUG_002582"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="ACHAUG_002583"
     CDS             complement(5146..5517)
                     /locus_tag="ACHAUG_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8481629.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="ACHAUG_002584"
     CDS             5732..5917
                     /locus_tag="ACHAUG_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481630.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="ACHAUG_002585"
     CDS             complement(6000..6188)
                     /locus_tag="ACHAUG_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481631.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="ACHAUG_002586"
     CDS             complement(6212..6475)
                     /locus_tag="ACHAUG_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481632.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="ACHAUG_002587"
     CDS             6633..6890
                     /locus_tag="ACHAUG_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8481633.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="ACHAUG_002588"
     CDS             complement(6961..7152)
                     /locus_tag="ACHAUG_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481634.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="ACHAUG_002589"
     CDS             complement(7293..7613)
                     /locus_tag="ACHAUG_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481635.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="ACHAUG_002590"
     CDS             complement(7636..8070)
                     /locus_tag="ACHAUG_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8481636.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..>8586
                     /locus_tag="ACHAUG_002591"
     CDS             8405..>8586
                     /locus_tag="ACHAUG_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8481637.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2719 a         1694 c         1361 g         2812 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctgaggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattc
//
LOCUS       DAYYBH010000101         8494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000101 DAYYBH010000000
VERSION     DAYYBH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.104"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHAUG_002592"
     CDS             complement(<1..149)
                     /locus_tag="ACHAUG_002592"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8481638.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHAUG_002593"
     CDS             complement(593..1714)
                     /locus_tag="ACHAUG_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM8481639.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHAUG_002594"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHAUG_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM8481640.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHAUG_002595"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHAUG_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM8481641.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHAUG_002596"
     CDS             complement(4304..5215)
                     /locus_tag="ACHAUG_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8481642.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHAUG_002597"
     CDS             complement(5232..6239)
                     /locus_tag="ACHAUG_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8481643.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHAUG_002598"
     CDS             complement(6236..8365)
                     /locus_tag="ACHAUG_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8481644.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACHAUG_002599"
     CDS             complement(8370..8480)
                     /locus_tag="ACHAUG_002599"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481645.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAYYBH010000102         8310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000102 DAYYBH010000000
VERSION     DAYYBH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.105"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHAUG_002600"
     CDS             476..1339
                     /locus_tag="ACHAUG_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8481646.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHAUG_002601"
     CDS             complement(1468..3090)
                     /locus_tag="ACHAUG_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8481647.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHAUG_002602"
     CDS             3339..3860
                     /locus_tag="ACHAUG_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8481648.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHAUG_002603"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHAUG_002603"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8481649.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHAUG_002604"
     CDS             5941..6759
                     /locus_tag="ACHAUG_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8481650.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHAUG_002605"
     CDS             complement(6862..7059)
                     /locus_tag="ACHAUG_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481651.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHAUG_002606"
     CDS             7228..7806
                     /locus_tag="ACHAUG_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8481652.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHAUG_002607"
     CDS             7901..8038
                     /locus_tag="ACHAUG_002607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481653.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2504 a         1489 c         1638 g         2679 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacat cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg
//
LOCUS       DAYYBH010000103         8248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000103 DAYYBH010000000
VERSION     DAYYBH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.106"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..1559
                     /locus_tag="ACHAUG_002608"
     CDS             234..1559
                     /locus_tag="ACHAUG_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8481654.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="ACHAUG_002609"
     CDS             1552..1902
                     /locus_tag="ACHAUG_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481655.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="ACHAUG_002610"
     CDS             1899..2069
                     /locus_tag="ACHAUG_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481656.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /gene="prgO"
                     /locus_tag="ACHAUG_002611"
     CDS             complement(2973..3248)
                     /gene="prgO"
                     /locus_tag="ACHAUG_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8481657.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="ACHAUG_002612"
     CDS             complement(3220..4173)
                     /locus_tag="ACHAUG_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8481658.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="ACHAUG_002613"
     CDS             4785..6278
                     /locus_tag="ACHAUG_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8481659.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="ACHAUG_002614"
     CDS             6413..6709
                     /locus_tag="ACHAUG_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8481660.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="ACHAUG_002615"
     CDS             6810..7661
                     /locus_tag="ACHAUG_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481661.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7894
                     /locus_tag="ACHAUG_002616"
     CDS             7682..7894
                     /locus_tag="ACHAUG_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481662.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2863 a         1283 c         1552 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgac
//
LOCUS       DAYYBH010000104         8091 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000104 DAYYBH010000000
VERSION     DAYYBH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.107"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            267..968
                     /locus_tag="ACHAUG_002617"
     CDS             267..968
                     /locus_tag="ACHAUG_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8481663.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1577..2593)
                     /gene="tsaD"
                     /locus_tag="ACHAUG_002618"
     CDS             complement(1577..2593)
                     /gene="tsaD"
                     /locus_tag="ACHAUG_002618"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8481664.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2590..3054)
                     /gene="rimI"
                     /locus_tag="ACHAUG_002619"
     CDS             complement(2590..3054)
                     /gene="rimI"
                     /locus_tag="ACHAUG_002619"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8481665.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3061..3603)
                     /gene="rimI"
                     /locus_tag="ACHAUG_002620"
     CDS             complement(3061..3603)
                     /gene="rimI"
                     /locus_tag="ACHAUG_002620"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8481666.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3587..4309)
                     /gene="tsaB"
                     /locus_tag="ACHAUG_002621"
     CDS             complement(3587..4309)
                     /gene="tsaB"
                     /locus_tag="ACHAUG_002621"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8481667.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4500..5480)
                     /locus_tag="ACHAUG_002622"
     CDS             complement(4500..5480)
                     /locus_tag="ACHAUG_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8481668.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5504..6988)
                     /gene="galT"
                     /locus_tag="ACHAUG_002623"
     CDS             complement(5504..6988)
                     /gene="galT"
                     /locus_tag="ACHAUG_002623"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8481669.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(7000..7989)
                     /gene="galE"
                     /locus_tag="ACHAUG_002624"
     CDS             complement(7000..7989)
                     /gene="galE"
                     /locus_tag="ACHAUG_002624"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8481670.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2292 a         1717 c         1415 g         2667 t
ORIGIN      
        1 tcattgaaaa aatttttttg aataaattaa aaaaataaat tatattatcg ttgttagcgt
       61 ttactatatg tattattgtt gttttttata cacaaatcat tcgcttcaaa acaaattatt
      121 caataccatt gtatattttt tacataataa caaattatat ttgtaaagta ttaaaacttg
      181 tttctaatgt tggtatgtta aaactttaca aattctttaa aaaaagtgac aataaatata
      241 gtgaacaata aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa
      301 aactcagata tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg
      361 aacatactgt tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca
      421 gacaacggtt gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg
      481 caacttatta ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt
      541 ttggagaaga gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc
      601 ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga
      661 tacaaaaaat ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct
      721 ctagcgcctt tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac
      781 caacaaaaga tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga
      841 atagcggcac gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccgggc
      901 tgttagatta tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga
      961 gagaataacg cgaacaaaaa taagcagtga ccctgataaa tatcagggtc actgcttatt
     1021 ttcctcccta aaacttctga aagatccggc agttttagat cactggacaa gactcaaaga
     1081 ttttctatgt gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgttagatta
     1141 actagatgaa gcgtcttgtc ctcatccgat ctagattatt cgatattcct cgacaataaa
     1201 cgattactca gtgagggcaa tcagaggaac ggataatgat aaataagctg taaaaaatag
     1261 caaaataaca ataatgatta aaagtatgta aaaaataggg ctttgacaaa aacgtgtcac
     1321 agccctattt cgattaaacg gtgtgtacct gtcaactctt cggtcacaag tcataatgtt
     1381 cataaacatg aggagctgtt ttttgaaatt gttccttgtg aatcagagtt aaacgacagg
     1441 cttacgaact cttgattaaa cggcgttcaa aagcttgcat acgcggtaat aagaacagcc
     1501 aggtcaaaaa catgagaagc cgtttttcct gggctgttct tattacttga tgcagggcag
     1561 ctttttcaca acctctttat tcagataaaa ccaagcttgg atcagcattc aactgatagc
     1621 tagcaaaatt tcttttttgc atttccacat gtgctgctgc tccaatcatt gcagcattat
     1681 ctccacaaag tcgaagaggg ggaatcacta gttctacctc agggagttta gttgccaaag
     1741 catcctgcaa tccttcacgc aacccttgat tggccgctac acctccagct ataatcagtt
     1801 gcttgactgg ataattttga caagctcgaa gtgttttagt aatcaataca tcgatcacac
     1861 ttgcttgaaa gctagctgct aaatcatttt tgtctagatc ttctccacgc tgttgtgcat
     1921 tatgcaccaa attgataaat gcacttttta atccgctaaa actgaaatca taattatctt
     1981 catggatcat cgctcgcgga aaatggtaat tatcttttcc ctgatgagct agctgatcga
     2041 tttcttttcc gcttgggtaa gaaagaccta atactcttcc aactttgtca taagcttctc
     2101 ctgctgcgtc atctcttgtc tccccaatga tttcatatga tccatcttct tgcatataga
     2161 ctaattctgt atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa
     2221 ccagacgggc tgcatagata tgccctgcca tatggtttac aggaataagc ggcaattggt
     2281 gtgcccaagc aaaagcctta gctgcagaaa taccaatcag caacgagccg acaagtcctg
     2341 gtccataagt gacagcgact gccgataaat cttctgccga cacaccagct tctaccagtg
     2401 catcttccaa gcataaggtg atttgttcta cgtgatggcg gctagctact tcaggtacta
     2461 cgccaccaaa acgtttatga ctgtttattt gagaagcaac gatgttagat aagatctctg
     2521 ttccatttct aacgaccgct acactcgttt catcacaact tgattcaatc gccagaatca
     2581 attcttcatt catttcatcg tctccgtttt taactttctg ctcataatga tcgcatcttc
     2641 cgtcggattg cgataatatg atttccgttt acctaatata cgaaactttt ctgattcata
     2701 aagttttctt gctgcttcat tagatagacg tacttctaaa aaaacatgca gcgaatcttt
     2761 tatttgttcc gtaatcagta attgtaacaa atgccgtgca tgcccttttc cttgttctga
     2821 aactgcaaca gcaatatttg tgatctcgac ttcatctatt actttcgagt atccaataaa
     2881 gccacagatc tggttatctt cagctaaaag cagataatca gagtgaggct gttggatgtc
     2941 tgccaaaaac tgcattttcg tccaaggtga accatgtgga taagcatttt cgctaatcat
     3001 ccatacgcat tctgccaagt acgcttcatc aaaatctttt tttctgcaaa tcaactaatc
     3061 tcactcattt tctaaaagtt ttatcatatc caacgcatct tcttttgttt cagtgtaata
     3121 tctttttttg atcgttgttg cttgaaaccc caatttccga taaagtcttt gtgcatcttg
     3181 attactcata cgcacttcta atgacatcgt ctcacagcga ttcattatag caaagttctc
     3241 gatttcgtca ataagcagcg aaccgattcg tttacgctga tatgccggtc gaacggcaat
     3301 attcgtgata tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc
     3361 attatcaaag atacctagat atagatgagg aagcggtgac ttcaattctg atataaaggc
     3421 actttttgtc catggcaatt cgccattata gacttctcgt tcgacatcca gcagctcttt
     3481 gatatcttct gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt cgactgtttt
     3541 ttctgtataa atcattttat cttgaaaaaa gcttctggca tacgctctaa attttttcaa
     3601 cataactttc gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct tctactcgtt
     3661 ttaaatagcg aggaagaaaa gcttgtttct ctgcaactgg ttcaactaaa gcacctaatt
     3721 gagccacagt tacaccactt ggaatatccc atgctggaac tgtattaatc atcgcactag
     3781 gcaatatttc ctttatttca gagaagaatt tgtgacaatc actcccgacg aaataaatag
     3841 cttcattcag cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa
     3901 gtacagtttc taatctattt tctttccagc ggtatgcccc agcgtataca ttttttcttc
     3961 gagcatcaaa catgggcaca ataatattat ccacaccgat gcaattagcc gcgatggttt
     4021 tcagactaga tactcctact agctctttat ctaatgtata agcaatcgtt tttgctgtcg
     4081 ttacacctat acgaagccct gtataagaac cgggaccatc agaaaccgcg attcggtcaa
     4141 tgtcttttgg acttatttga agatctgcaa acaattggtc gattgcaggc atcagtgtta
     4201 cactgtggtt gcgtttgata tttgtttgta tttgtccaag aatttgttcg tcttctacca
     4261 caccgatcgc caaagtttgg tttgcagtat ctattcctaa tgtaatcata tatttgatcc
     4321 tttctgctat gacattctcg ccttccattg tagcatactt ttttattctg aggatgaaaa
     4381 taccaaaggc aaaactagta caataataca tgattatctg ctggcgggca aattttgccc
     4441 cttcctgaaa cacaaaaaag ctgcggtatc actccatttt cccacagctt ttccatcttt
     4501 tatttcgttg attctcgaat aatcaactct gtttccacaa taatctttct tgaaaccggt
     4561 gctttggaat tacttaattg aagaatcgtc tccactgcta gctctcccat ccattcggta
     4621 taaactttta cagtagaaag cgagggcgtt acatatttcg ctacactgac atcattgaag
     4681 cccatcacag aaatatcttc tggtaccact aaaccttttt cttgaatcgc tttcagtgcg
     4741 ccaatcgcta atgcatcatt agctgcaaaa agcgcatcag gaacatgcgg attcgttttt
     4801 aaatactgct tcactgcttg atatcctgac tcgacagaaa aatctgcttc gataattgga
     4861 ccttcgtcgt agcctttttc tgccaaaact tgttgaaatg ccataattct gggatctttt
     4921 agccgctgat ggtttttctt cgtatattct gttccagcaa gcatagcgat atgttgcgct
     4981 ttatgggaga caaagtgttc aatgactttt tgaacacttg acttaaagtc gacaactaga
     5041 gagtcgattc cttgagaagt gccgtcagaa tctaccaaaa gcaaggcatt ttgggatcct
     5101 ttcatttcta ataactgagc ttcgtcgaat tttcctagtg cgatcgttcc atctgccgga
     5161 agatcactaa tggaatccca cgtttctttc aataaagtaa cgtttgcttc ttctgccttt
     5221 ttttcgattc ctaggcgaat agataaataa taaagatctt caagctcttc cgtatcgtcg
     5281 taccactgga tcaggcgaag cgtttgctta gtatttgctg catttttacg atgtttcgta
     5341 taatttaagc gttctgctgc ttcaaaaatt ttttgctttg tggctaagcc aacggaaaga
     5401 tcaggatcat agttgagaac tcttgaaaca gtagcaggag aaactcctgc caactgcgca
     5461 atgtctttaa ttgttgccat ttgtctctcc tcctttttta cgtttataaa atcgcagtaa
     5521 atcgttcaaa cgctgcctgt cctttttcat ctcgcttgaa tactccagcg tcttccagta
     5581 cacgtgcaaa aacctgacct acagattttt gtacgacatc ggtgaccgta tctttcgtca
     5641 aatcaggatg ttcttttttt aattgttctg cccacggacg atgatattca gccatctggt
     5701 tttcttctcc taataagaaa tgctctactt ctactagttc tggtttcaaa cgaggtggta
     5761 aaacagcaag ccccattact tcgatcaacc caatgttctc ctttttgata tgttggacat
     5821 ctttatgtgg atgaaaaatc ccatcaggat gttcttctga aacattgttg tctcgaagga
     5881 caaggtccaa ctcaaagtaa ttgtcccttc ttcgtgcaat tggcgtaatc gtatggtgtg
     5941 gtgtaccatc tgctgaaaaa gctctgatag atactgtttc atccgaatat gctttccatt
     6001 tttccaatac tgagttagct gcttcaataa gcaagttttt ctctgttgat tgcaaacgga
     6061 tcacagacat cggccatttc acgactcctg cgatgacttg cggataatct tctagtttga
     6121 taagtttttc tatcggtgct tttgccatag gaaattcatg acgtcctgcc tgataatgat
     6181 catgagaaag aatcgatcct ccaacaattg gtaagtcagc attcgaacca acaaagtaat
     6241 gaggcaagac ttcagtaatc cgaagcagtc tagcaaatgt ttcctttgtg attttcatcg
     6301 ggcggtgttg ttctgataaa atgatcgaat gttcattgta ataagcataa ggggaatatt
     6361 ggaatcccca gctttcaccg tctagattca ttcgaataat ccggtgattg gtgcgggctg
     6421 gatagttcaa gcgacctcta tatccttcgt tttccatgca aagcatgcag gtaggatagt
     6481 ctacttttac cgcctcgcgt tctgctgcga tttgttttgg atctttttct ggcttagata
     6541 aattgattgt gatttccagt tcaccatact ccgactctgc cggaaatttg atgttttttg
     6601 cgatggctct tgttttgatg tagtcatttt tcttacttaa attaaagaaa tactcagttg
     6661 cttcctctgg gcttttcgaa tagtgctgtg caaagaatgc attgacgact gaaggtggcg
     6721 gtgtgagaaa atccattaat tgcgcttcaa aaatctcttg ttctgctggc aattctgaaa
     6781 gaacaccatt ttctcttgcc ttagcgacaa gctgatccaa taagtctaca gattcttcct
     6841 taactggtct tacttcaatg ctttctagag catcttctcc taccatccct aaaagacgat
     6901 tctgtaaata taagcggtcc atttccatcc atccgccagc ttcgatcgca agtgtggcaa
     6961 aatcacaaat cgtttgactg attgacatat ctcattcccc tattcttcat aaccatttgg
     7021 atggctgaca tgccaatccc atgctgtttt gataatcgct ttgatatctg tgtattcagg
     7081 tttccagccc agaatctctc tcgctttttc acttgaagcg actaatcggc taggatctcc
     7141 agctcttctt ggtgctattt ctgctggaat ttcttttcct gtcacttctc tagctgcttc
     7201 tagcatttcc ttgactgagt atcctttgct gctgcctaaa ttgaagaagt tgctttcatt
     7261 tccttctttc aagtattcca aagctaaaat atgtgcagca atcaaatctt ctacttgaac
     7321 ataatctctg atacatgttc catctggtgt atcataatcg tctccataaa cagccaaggc
     7381 ttttctttga ccaagagcta cttgtaaaat gattgggaca agatgcgttt ctggtgtatg
     7441 atcttctccg atagatgcat ctgcttttgc tccggctaca ttgaagtagc gcaaagcgac
     7501 atagcgcatg ccatatgctt ggtcacacca cttcatcatt ttttccatca tcaatttact
     7561 ttcaccatac ggatttttag gattagctgg tgtatcttct gtgattggag actctttcgg
     7621 ctctccataa gttgctgcag tagaagagaa tacgatttta ttcacgttga actcgtgcat
     7681 gacttcaagt aagatttgca taccgtatac gttgttgttg aaatacatca atggtttttc
     7741 gacagattct cctactaaag agcttgcagc aaaatggatt actccttcaa tcggttcctt
     7801 ttcaaatacg gaacgtagaa attctttatc ccggatgtct ccttcataaa aatgagcatc
     7861 tggatgtact gcttgtttgt gacctgtgag caaattatct accacagcta cttgataccc
     7921 cttttgaact agctggtcta cagcgtgcga accgatataa ccggctccgc ctagtactaa
     7981 aattgtcata aaaaacactc ctttgattag taaaattgtg tttgttgatt ctatataatt
     8041 taaaaaacga agtttacttt tatggtgaaa acaaacaaag taaaatttcg t
//
LOCUS       DAYYBH010000105         8023 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000105 DAYYBH010000000
VERSION     DAYYBH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.109"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..1797
                     /locus_tag="ACHAUG_002625"
     CDS             259..1797
                     /locus_tag="ACHAUG_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8481671.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1794..2759
                     /locus_tag="ACHAUG_002626"
     CDS             1794..2759
                     /locus_tag="ACHAUG_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8481672.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2746..4068
                     /locus_tag="ACHAUG_002627"
     CDS             2746..4068
                     /locus_tag="ACHAUG_002627"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8481673.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4131..4235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4309..5052
                     /locus_tag="ACHAUG_002628"
     CDS             4309..5052
                     /locus_tag="ACHAUG_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8481674.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5067..6059
                     /locus_tag="ACHAUG_002629"
     CDS             5067..6059
                     /locus_tag="ACHAUG_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8481675.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6071..6808
                     /locus_tag="ACHAUG_002630"
     CDS             6071..6808
                     /locus_tag="ACHAUG_002630"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8481676.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6798..7520
                     /locus_tag="ACHAUG_002631"
     CDS             6798..7520
                     /locus_tag="ACHAUG_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8481677.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7604..>8023)
                     /locus_tag="ACHAUG_002632"
     CDS             complement(7604..>8023)
                     /locus_tag="ACHAUG_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8481678.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2570 a         1389 c         1751 g         2313 t
ORIGIN      
        1 aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag
       61 atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct
      121 tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt
      181 aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat
      241 aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag
      301 ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca
      361 ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta
      421 ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc
      481 ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt
      541 ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca
      601 gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca
      661 ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact
      721 ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga
      781 aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc
      841 agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca
      901 gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa
      961 accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa
     1021 gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg
     1081 agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt
     1141 actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa
     1201 gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta
     1261 aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa
     1321 catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc
     1381 acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg
     1441 aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga
     1501 gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg
     1561 actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa
     1621 gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata
     1681 acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca
     1741 gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta
     1801 aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc
     1861 taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa
     1921 aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact
     1981 tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg
     2041 tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc
     2101 ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa
     2161 aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag
     2221 aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg
     2281 acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt
     2341 ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa
     2401 tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac
     2461 cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga
     2521 aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa
     2581 taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc
     2641 ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga
     2701 caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat
     2761 gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca
     2821 ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga
     2881 tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg
     2941 agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa
     3001 cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc
     3061 gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa
     3121 gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg
     3181 gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa
     3241 gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct
     3301 gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg
     3361 tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt
     3421 gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg
     3481 gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta
     3541 gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg
     3601 caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa
     3661 ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca
     3721 agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct
     3781 gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt
     3841 accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt
     3901 gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc
     3961 gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc
     4021 atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa
     4081 ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc
     4141 tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag
     4201 ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc
     4261 atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat
     4321 ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt
     4381 tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca
     4441 gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg
     4501 gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta
     4561 gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg
     4621 ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg
     4681 ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag
     4741 caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa
     4801 ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc
     4861 agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata
     4921 cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta
     4981 ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt
     5041 tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt
     5101 tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt
     5161 tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc
     5221 tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag
     5281 gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt
     5341 atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg
     5401 ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt aacatccatt
     5461 caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg
     5521 tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa
     5581 gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag
     5641 gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag
     5701 aattagatga acggttagat tactatactc cggtaattat tacgaatgat caactgatca
     5761 aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag
     5821 ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc
     5881 ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga
     5941 attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg
     6001 gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg
     6061 agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat
     6121 attagaagac atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag
     6181 cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg
     6241 aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc
     6301 gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa
     6361 aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt
     6421 aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc
     6481 cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc
     6541 gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc
     6601 aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag
     6661 gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa
     6721 acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt
     6781 attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt
     6841 tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag
     6901 aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc
     6961 atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa
     7021 ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa
     7081 aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc
     7141 acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt
     7201 tgatccattg gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt
     7261 tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc
     7321 caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg
     7381 ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg
     7441 agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt
     7501 tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg
     7561 ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg
     7621 cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg
     7681 ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt
     7741 aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga
     7801 tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag
     7861 caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa
     7921 caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt
     7981 gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAYYBH010000106         7601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000106 DAYYBH010000000
VERSION     DAYYBH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.110"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHAUG_002633"
     CDS             complement(138..428)
                     /locus_tag="ACHAUG_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481679.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHAUG_002634"
     CDS             complement(546..806)
                     /locus_tag="ACHAUG_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481680.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHAUG_002635"
     CDS             complement(943..1356)
                     /locus_tag="ACHAUG_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8481681.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACHAUG_002636"
     CDS             complement(1812..2297)
                     /locus_tag="ACHAUG_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481682.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACHAUG_002637"
     CDS             complement(2436..2651)
                     /locus_tag="ACHAUG_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8481683.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACHAUG_002638"
     CDS             2875..3675
                     /locus_tag="ACHAUG_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8481684.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACHAUG_002639"
     CDS             3694..5562
                     /locus_tag="ACHAUG_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8481685.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACHAUG_002640"
     CDS             5555..6046
                     /locus_tag="ACHAUG_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8481686.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACHAUG_002641"
     CDS             6034..6588
                     /locus_tag="ACHAUG_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8481687.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="ACHAUG_002642"
     CDS             6606..7601
                     /locus_tag="ACHAUG_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8481688.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DAYYBH010000107         7537 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000107 DAYYBH010000000
VERSION     DAYYBH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.111"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACHAUG_002643"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACHAUG_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8481689.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACHAUG_002644"
     CDS             1483..1935
                     /locus_tag="ACHAUG_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481690.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACHAUG_002645"
     CDS             1949..2638
                     /locus_tag="ACHAUG_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481691.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACHAUG_002646"
     CDS             2763..5114
                     /locus_tag="ACHAUG_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8481692.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACHAUG_002647"
     CDS             5194..5589
                     /locus_tag="ACHAUG_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481693.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACHAUG_002648"
     CDS             5914..6531
                     /locus_tag="ACHAUG_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8481694.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACHAUG_002649"
     CDS             6572..6871
                     /locus_tag="ACHAUG_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFM8481695.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACHAUG_002650"
     CDS             6905..7072
                     /locus_tag="ACHAUG_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8481696.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACHAUG_002651"
     CDS             complement(7117..>7537)
                     /locus_tag="ACHAUG_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8481697.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAYYBH010000108         7217 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000108 DAYYBH010000000
VERSION     DAYYBH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.112"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACHAUG_002652"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACHAUG_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8481698.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACHAUG_002653"
     CDS             complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACHAUG_002653"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8481699.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACHAUG_002654"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACHAUG_002654"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8481700.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACHAUG_002655"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACHAUG_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8481701.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACHAUG_002656"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACHAUG_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8481702.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="ACHAUG_002657"
     CDS             complement(4433..5290)
                     /locus_tag="ACHAUG_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8481703.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACHAUG_002658"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACHAUG_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8481704.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DAYYBH010000109         6751 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000109 DAYYBH010000000
VERSION     DAYYBH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.114"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..905
                     /locus_tag="ACHAUG_002659"
     CDS             15..905
                     /locus_tag="ACHAUG_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8481705.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(978..1802)
                     /gene="nadE"
                     /locus_tag="ACHAUG_002660"
     CDS             complement(978..1802)
                     /gene="nadE"
                     /locus_tag="ACHAUG_002660"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8481706.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1814..3286)
                     /locus_tag="ACHAUG_002661"
     CDS             complement(1814..3286)
                     /locus_tag="ACHAUG_002661"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8481707.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3923..4285)
                     /locus_tag="ACHAUG_002662"
     CDS             complement(3923..4285)
                     /locus_tag="ACHAUG_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8481708.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4508..5260
                     /locus_tag="ACHAUG_002663"
     CDS             4508..5260
                     /locus_tag="ACHAUG_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8481709.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5400..6323)
                     /locus_tag="ACHAUG_002664"
     CDS             complement(5400..6323)
                     /locus_tag="ACHAUG_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8481710.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6338..6610)
                     /locus_tag="ACHAUG_002665"
     CDS             complement(6338..6610)
                     /locus_tag="ACHAUG_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481711.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2061 a         1348 c         1156 g         2186 t
ORIGIN      
        1 aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta tatgaagcat
       61 tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag atgaatgtca
      121 aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc
      181 tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc
      241 gtgtcgataa aatcgatatt agttttccag aaaaaatatt caatgctttt gatacgaaag
      301 aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca tgcggtttag
      361 ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg gatcctcgaa
      421 ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag gcacctaatt
      481 ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt tcttctgaat
      541 ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat ggtgtggagc
      601 taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat acgcctgatt
      661 ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt accaaacact
      721 tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc cctaaagagc
      781 aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg gggggctgta
      841 ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa gtttactaca
      901 gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat gaagggacgg
      961 ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt
     1021 ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc gggaacttct
     1081 tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac ttcatctgca
     1141 atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa tttttccggt
     1201 gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa
     1261 atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc tgcagcatga
     1321 tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt aatcatacgt
     1381 tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat
     1441 acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac atcgggctgg
     1501 ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata aggaagacga
     1561 acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc catcgttagt
     1621 tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag tacaaaagtt
     1681 ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt acgtatttct
     1741 tcttttggat caattgttgg tttgacacct aattcattga taatttctgt ctgcaggctc
     1801 atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc attcgttcca
     1861 tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat ttttgcggat
     1921 tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta tattctttga
     1981 tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata tcttgtaaaa
     2041 ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact ggatggaaca
     2101 tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag tctccttcag
     2161 atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga gtagttactt
     2221 tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca tcttcgatcg
     2281 atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc aattttgtcc
     2341 caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc gttgcttcat
     2401 ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct aattgttccc
     2461 gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct tggaagttga
     2521 ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat
     2581 cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc ataaatgctt
     2641 cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta gctggtatcc
     2701 caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata tatgccgctc
     2761 tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat tccaatagtg
     2821 gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat
     2881 tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc ccttccactt
     2941 ggatcaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga actgttgctt
     3001 taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct aatccacgca
     3061 agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact aaccgttcca
     3121 atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa
     3181 cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg atttgatata
     3241 gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg tattactcct
     3301 cttggcaagt tttcatttcc tacccattat aacaaaaaaa attaatagga aaagaaatct
     3361 tccatttttt ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa tgaactccat
     3421 ctgctaaaac tatcctttct tattggtatt taacctaaac gagaaagaat acttatttta
     3481 ttcaatgtat ccgagacacg aagaaattag cgttttctat ttttcttact tgataaaata
     3541 agtagatttt cctactacaa tcgtagccgt taagataccg atgattgaac aaatataacc
     3601 aaatgtctct acaacataat caatggcaaa agtttttgaa gcaataatac gtctttcctt
     3661 ttttgcttct tatccccaaa gaaacggata taacttatta tgtattgaat ttttttggaa
     3721 aggcgaatta tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg
     3781 tttttaaaaa gatacaaagt aagaaagcag ctgtcaatag aatggaaaca aaaatggcga
     3841 gacatcaatc agtctcgcct tgttttttat tgtatacatt agatatggag caggttacca
     3901 taaagatgat tattagtcgt tattaaatag aatccttcgt atttttttgt tctatcacta
     3961 caatatcttt atttggacgt ttgaattgta ttccggcacg ttctagctcc tgtttcactc
     4021 ctacatggac tttgacatct tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga
     4081 tttcttgcct tgtgattttt ttcttcgtat tgatcatcac gacttgcttt ttatcatatg
     4141 tgtctaccaa tagcagttgt gttcgatcta acgaaaataa tttgtaataa ttaatagccg
     4201 tattcccaaa aaagaattcc actactttag gataaaatgt cttaatattt aaattactat
     4261 tcacaggtgt ttcgattagc ttcatctcat tcctcttttc tatatagtcg atttagatcc
     4321 aatcttaagt tatgttacca tctttcctag cattaaggaa tcggttacaa ttgaaccttt
     4381 aaagacaaaa ggatttacct tttatcactt aatagtataa cataatttca tgagaaaaaa
     4441 atacaatttt ttcttgacca tctcgttacg taaaggatta tgcttacatt tgtcaggagg
     4501 aaaaataatg gagtacacaa taaagaaaat gtcagagata tcaggagtca gtccgagaac
     4561 cttgcgcttt tatgatgaga ttggactgct aaaaccagct aggatcaatt cttcaggtta
     4621 tcggatttat ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt atcgaacaat
     4681 ggcattcaag ttggatgata tccaagaggt cttagacaat ccatcttttg accaccagaa
     4741 agctttaatc aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt
     4801 gacaactgtc caacagacac ttgatatgta cgaaggagga agaaaaatga gtgacaaaga
     4861 aaaatttgaa gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct atggacaaga
     4921 gattcgacaa aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat
     4981 ggataaagaa acgtatgaag aaatgcaaac tgttgaaaag cagatgttat ttgatctcac
     5041 tctttactta aaagaaccag aagatgaaag cttagcagac cgtatcttcc aagctcataa
     5101 gaagtggtta tcttacagct ggcctgaata tcagccaaaa gcgcataaag gactaggaat
     5161 gatgtacgaa gccgatgaac ggttcactgc atattatgac gagcgaagtg gtcatggtgc
     5221 agcaaaagct ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa
     5281 aaaagtacac gtaaaatgac ctaataacaa aacagcagac acccaagtta gatttttatg
     5341 ctaacttttg gtgtctgctc cattctttca caagtttttt catcctactt taaatatatt
     5401 catcgttgaa taaacgtata aggtattttc actttatcct ttttttctgt aagaaacaat
     5461 tgaaatgcca tttctccgca agcttccaga tggtggtcga tggttgggaa accaagaagc
     5521 tcgctgatca gcaaattatc gcgtccaatc accactggtg tcttatcttg cagatagctt
     5581 tgcaagatgc ctgctgcaac catatccgaa tttgtaaaga caccatcaac tccaagctct
     5641 ttaaaaaaga agcctgcttg ttttcctttc tctgcatcta tacaatccca aaaaacaagt
     5701 gattcatcaa aagatggaaa aagttgatga catagctgca aagtgatttt tgagttataa
     5761 cttagcctgc cacttcgccc caacgtgatg cccaattgct ttataccttg ctgcttcaaa
     5821 taagtaagac cttctgtaat tgattgttct cgatcaataa agacacagct tgcctgtttt
     5881 cccttgattt cttcacaaaa aactagaggt ccatattttt gatatgccaa taaatcatct
     5941 agtgtattgg cacgagacgt aatgatcaat ccatcgaaag ctttagctgc aaattcttca
     6001 agataccttc gctctttttc tttctgatag cccgtcggta aaagtgtcac tttgtaatca
     6061 taatcaaacg ctgcttcaat gatcccactg atcaactgat caaaatacgg atgattggta
     6121 taaggcagaa tcactccaat gcttttcgtt tttccataac ttaactgtct ggcatttgca
     6181 ttagggatat aatcgacttc tttcatgacc gcaagtattt ctttcctttt ttcctcatct
     6241 acatatggat gtccattgat tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt
     6301 gcgatatctc gaatgtttgc cactgctgct tcgccaccta tacaaaaaga ttttcttgta
     6361 aatagtctac cacagctgga ccgcattcaa aaaactgata catttttcct tcatgcgaaa
     6421 aactgatccg ctgcccgccc caatatatat cttttgtcaa attttcgatt tcattcattg
     6481 gtatttcttt tactatattc ctattcaacc cttgaaaact gtacaacgtt aatacggatc
     6541 ccttgaactc gacaaacgca taaaaataat tgttcgttgt gccatctgtt atctctacat
     6601 aagtattcat tgcaagccct ccttttgata ctttgagtat agtgtatgaa acgcgtctca
     6661 tagcaaggag tttgttttgc caaagaaaag ttttattcct ctttcattat taccaaagtc
     6721 tcttatttta aattgaatac attataatga t
//
LOCUS       DAYYBH010000110         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000110 DAYYBH010000000
VERSION     DAYYBH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.115"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHAUG_002666"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHAUG_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHAUG_002667"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHAUG_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8481712.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHAUG_002668"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHAUG_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHAUG_002669"
     CDS             complement(2143..2373)
                     /locus_tag="ACHAUG_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8481713.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHAUG_002670"
     CDS             complement(2603..3421)
                     /locus_tag="ACHAUG_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8481714.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHAUG_002671"
     CDS             3582..4271
                     /locus_tag="ACHAUG_002671"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8481715.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHAUG_002672"
     CDS             4285..5787
                     /locus_tag="ACHAUG_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8481716.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHAUG_002673"
     CDS             5800..6276
                     /locus_tag="ACHAUG_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8481717.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHAUG_002674"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHAUG_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYBH010000111         6466 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000111 DAYYBH010000000
VERSION     DAYYBH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.116"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHAUG_002675"
     CDS             complement(99..1229)
                     /locus_tag="ACHAUG_002675"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8481718.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHAUG_002676"
     CDS             complement(1878..3080)
                     /locus_tag="ACHAUG_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8481719.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHAUG_002677"
     CDS             complement(3140..3343)
                     /locus_tag="ACHAUG_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8481720.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHAUG_002678"
     CDS             complement(3485..4552)
                     /locus_tag="ACHAUG_002678"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8481721.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHAUG_002679"
     CDS             complement(4610..4717)
                     /locus_tag="ACHAUG_002679"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481722.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHAUG_002680"
     CDS             4880..5692
                     /locus_tag="ACHAUG_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481723.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHAUG_002681"
     CDS             complement(6066..6257)
                     /locus_tag="ACHAUG_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481724.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1950 a         1316 c         1011 g         2189 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaa
//
LOCUS       DAYYBH010000112         6378 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000112 DAYYBH010000000
VERSION     DAYYBH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.117"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            246..836
                     /locus_tag="ACHAUG_002682"
     CDS             246..836
                     /locus_tag="ACHAUG_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFM8481725.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            863..2173
                     /locus_tag="ACHAUG_002683"
     CDS             863..2173
                     /locus_tag="ACHAUG_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8481726.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2170..2889
                     /locus_tag="ACHAUG_002684"
     CDS             2170..2889
                     /locus_tag="ACHAUG_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFM8481727.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            2904..3989
                     /locus_tag="ACHAUG_002685"
     CDS             2904..3989
                     /locus_tag="ACHAUG_002685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8481728.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            4125..5657
                     /locus_tag="ACHAUG_002686"
     CDS             4125..5657
                     /locus_tag="ACHAUG_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8481729.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            5835..6056
                     /locus_tag="ACHAUG_002687"
     CDS             5835..6056
                     /locus_tag="ACHAUG_002687"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8481730.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         2229 a          686 c         1238 g         2225 t
ORIGIN      
        1 gtactattgt aaaaattcgt taggtttaaa taactggaaa tatcggaaaa tgggcattat
       61 acatcacgga catcccttct tttggaaaaa atgttacgat ttttataagt aatattgtat
      121 ccggtggaat caacgatgaa gataacatat ccatcgttgt agattcagta gcattgaatg
      181 atgctcttgt tgcaagaaat ccgaataaaa ttgttaagta taaattgaat taataggaga
      241 tatgaatgga aattagcagt aaagaatatt taaaagcggt tcttcgtatg gaaaagaatc
      301 tatatataga atctaaagga caacacacaa aattactaaa aataatgaaa tcacatccgg
      361 actacaacgc ttggcggtat actcgtcgaa tgagaattac atcttattat tattcaaaga
      421 gaaagaaaaa tatcatttat gcgattatgt atattgtgtc ttgcagaaga atgaatagtt
      481 tgggaagaaa atgtggtata gagtgtggtg aaaatgtatt tgatgaaggt gtaagatttt
      541 accatacaca aggaattgtt attaatggta acgccataat aggaaaaaat tgttacttat
      601 atgggaataa ctgtattgga aatgatggaa tcgatcctaa gtgtcctgta attggaaata
      661 atgtacgtgt atgcgtgggt gcaaagatta ttggtaatgt aaaaatagct aacaatgtag
      721 ttgttgcggc aggagctatt gttatcaaag attgtctaga gaatggtgca attcttgcag
      781 gtgtaccggc aaaaataata gggtatgtta atttaacaaa agtattttta gaataatttt
      841 tagcgaaaga aggtgaataa cgatgcgtat ttcaataaaa agatggtcag gagcagattg
      901 gacattgttc acgttggcat tttttgcaat ctccatagtt cttgtatata cttttaacgc
      961 cccaagagca attttatatt tgggagatat aattaatatc tttgtgttat taaatgcact
     1021 tagctttaga cgcagaaaag gaatatatgc tagcaaaaat gagatgctga tagttttgtt
     1081 tttactagtt tctatttgta gctcagttat taatttagaa aatataggat tgattatctg
     1141 ggggatacga aataacactc gtatgttcat agtatatata aattgtgcaa catatttaac
     1201 aattaaagat tactataaag tattcgctat aattgaaaaa ttattctgga tttcactgcc
     1261 actttgttta attgaaagat tcatggtcac ctatccaact ggaacaattg taggagatat
     1321 gataggtgga gttttttgga attattcagg ttgtaatatg ccattgaatt taattttgtg
     1381 tgtgacagtt actgttgtta caatcggata tttcaaggga gaaaaaaatt tcatccattt
     1441 tattttatgt gtttgtgcag cactttttat gtctgcatta gctgaattga aagtttttat
     1501 tcctgaatta tttataatat ttgttgcaat agtattcact ttaaaagttg attttaaagt
     1561 agtgatcaag atattatcat gcgtaatact tgtgtcgata gtattatcct tttttataac
     1621 aatatttatt acattgaacg ataatggaca aagctatgct gataatttca caattcaagg
     1681 tcttttagaa tatgcgtcaa gagattctgg atatgatggt gttggtgatt taaatcgaat
     1741 aactggtatc tctagagtag tagatgaaat atttaataat aattttagat tagtcctaat
     1801 gggagtaggc attggcaatg cagagtttac caattttttt gtcagtgagt tttataaatc
     1861 gtttggtaat cttcattatc agtattttca tcatatttgg ttatttatag aagttggaat
     1921 attaggagtt tcgatattcc tattttttat aataaagaca tttttcaatg caaaaagatc
     1981 ttttatgaag gataaagatg caagtcttgt taaagttatt gcaattatgt cggtattctt
     2041 ttttgtgtat aacacaacac ttaggggtga aacaacgggg ccactgattt atatgatttt
     2101 agccatgcct gaagttgtga aaaggaaaga gcattattat agtgaaaaaa atacaaaagg
     2161 agagatatta tgattcctaa gaagatacat tactgttggt tcgggggaaa tccacttccg
     2221 aaaaaagata gaaagtgcat agaaagctgg aaaaagtatt gccctgatta tgagattatt
     2281 gaatggaatg aatcaaatta tgatttaaat aagaaccgtt atatggccga tgcatataaa
     2341 gaacgtaaat ggggttttgt tccagattat gcaagatttg atattattta taatcatggt
     2401 ggattttatt tagatacaga tgttgagcta attaagtcgt tagatatact tcgtgaaaat
     2461 catgcctata tgggatttga aggggaaatc tgggttaatg gcggtattgg ttttggtgca
     2521 gaagaaggac attctgttat taaaggttta agggatattt acgattcatt aagtttttat
     2581 caaaatgatg gtacattgaa cttgaaaccg agtccgtatt atattacaga ttatcttgaa
     2641 tctattggat tggttcgcga taacagcgaa caaataataa atggcctgca tatctatcct
     2701 acggattatt ttgctgccaa ggattatgat actggaaaaa tttataaaac tagtaataca
     2761 ataagcattc atcaatataa tgcatcttgg aaaacaccta aacaaaaatt tgtttataga
     2821 ttaaaatcaa caattggaca aaaaaaattt aacaggttac ttgcactgaa aaaattttta
     2881 cacttataaa ggagaaaagt actttgaaga aaatttgttt cattagtggt gtaatttctc
     2941 gttctggggg aactgaaaga gtagggagta ttattgccaa tagcttgtct gatttaggtt
     3001 atgatgtcac tattttaagc ttttggaata gtggagagcc ttattttggg ttagataaga
     3061 cgattaaggt tgattatatg ttaaatccta aaacggaggg aaaattatat agaacttatt
     3121 tatatccggt ttggaaattg cacaagtata tatgcaaaaa tgaatttgat atagtaattg
     3181 atattgatac tttgttgtct ttgtatacta caagggcaat taaaggaaca aagtgcaaat
     3241 tgatttcttg ggaacatttt aattactgga caatggaaat gctgaaagaa aagaagagat
     3301 tcaaggctaa aaaattgata aaaaaatatg caaagaggct tgtggttctc acagaagaag
     3361 atcgtcaaaa acatattgat gaatatagtt tatcagaaga ttttgttata actatgccaa
     3421 atccgtgtat atctaatatc gagtacgaat atcattatga taaaaagaga tttcttgcag
     3481 tagggcgatt agtggaagag aaaaattttg ctgacatgat aagtgcttgg aaaattgtag
     3541 aaaaaaaagt agatgattgg gatcttatca ttgtaggaaa aggtgaatta gaaaaagatc
     3601 ttcgccagca gataaaagat ttaaatctgc aaagagtaac tatagcaggg cattcagacc
     3661 atgtggagga atattataaa tctgcatctt gttatgtgct tagttctgtt tatgaaggat
     3721 ttcctatggt tatattagaa gcacagtctt atggattacc agttatttct tatgattgta
     3781 aaactggtcc gagagatttg gtacatcata attttaatgg gatgttggtt gaagataaga
     3841 atatagacca attagctaag agcatgataa tgtttactaa gaatactgat ttagcaatga
     3901 gaatgtcact gaacgcatat aataatgtac aaaaatttaa tttgaaagaa attacaaaac
     3961 aatgggttgc tttgattgaa aatgtatagt tttttgtggt tgtccaaaca tgaaaattag
     4021 cgtaattaat caaagagcta gaagaaacct attacatttc aagattttag aaattatata
     4081 gtattaggta acaataaaaa atagaaagtt tggagccata tgatatgata attaatcgta
     4141 aacaaaatgc taaaaggaat ataattactg gaacaatttt taagttatat cagattatca
     4201 ttcccttttt tatgcgtaca cttatgattt atcagatggg aatggagtat gctggattga
     4261 acaacctgtt cacatctata tttcaaatgt taaatttagc ggaattggga ataggtgctg
     4321 cactaactgt aagtatgtat aaacctatag cagaagacaa tagaaaagag atttgtgctt
     4381 tactgaacgc atataaaaag tacttttcaa ttattggatg gatagtgttt acattaggat
     4441 tgttatgcat tccttttttg agatttttag ttaaaggttc gattcctgga aatttaaata
     4501 tgacagtttt gtatttaatg tatttgataa atactgtttt gtcgtattgg ttattttcat
     4561 atagaaagag tctaatatat gcacatcaaa gaaatgatgt aattaacgtg tctatgctaa
     4621 ttacttttac attccaattt gtaatgcagg catatgttct gattttctta aaaaattact
     4681 atttgtattt attggcttct attttgggac aaatagttct taacttaatg tgtgctgttg
     4741 tttctcaaaa gatgtatcct gattatactc ctgttggtga gttagagaaa gaaactattg
     4801 acgatattat aaaaaaagta aaaggattag ttacaaacaa ggtaggcggt acaatattac
     4861 gatcttcaga ttcaattatt atttcatctt tcttaggtct ttcagtactt gcgatttatc
     4921 agaattatta ctttattatt tctgccataa tgagtatatt tgcaataatt tatgaatcat
     4981 gtatagcagg tataggtaac agtcttattg tagaaagtca agaaaaaaat tatatagatt
     5041 tttgtgatat tacaacaatt ataggttggt ttctggcaat tagttgtgca tgctttatta
     5101 cattatttca gccgttcatg attatctggg tgggaagtga taatcttatg agtatttcat
     5161 ttgttatatt tatgatagtt cattttttct tatatgaaat tgatcagtta attgggttat
     5221 acaaagatgc tgctggaata tggtacgcag acaggtttag accattaatt acagcaatga
     5281 taaacattgt attaaatatc atcctcgtta attggttcgg attatatgga gttttggcgg
     5341 caaccattgt ttcagtattg ttggttgatt taccctggtt attatataat gttttccaaa
     5401 atatatttaa atttgaatcg gcaggtaaat atgtttggaa gctcataagt ttgtgtgtaa
     5461 tagcttttgt tgcttcttat ttaagttatt ttgcttgttc gtatgttaat atcggaaatt
     5521 tcaaagtgat aagtttagtg ataaatttag ttatatcggt tgtcatatcg attttagtta
     5581 ttcttctgtt taatatccgt aataagaagt ttaagtatat tttgaaagtt cttacgtcgg
     5641 tattggtaaa aagatgagaa gagacatcag aatgatatat aaagtaacta caatttttag
     5701 gctgccgaat aggcgaacga tgtatagttt ttgttccaac gaagactctt gttgaaattg
     5761 gagatgatat gaaggagtta caattttgac tcatgtttat gattgatcgg ttttaaaagt
     5821 agtatggaga agctttggga tcatcggaga ggatcaaaat aggtaataat gtttttatag
     5881 gaatgaagtc tagaatacta aagggtgtac atataggaaa taatgtagtt ataggtgcaa
     5941 atagtttagt aaacaaggac gtatcagacg attgtgttgt tgcaggaaat ctagcaaaag
     6001 taattatgtc cctagatgaa tattataaaa agataaacaa tatgaagaat ccgtaaaact
     6061 tgtgaaacta taccgagaat aatatgggaa aaatattgat gaaaaagcat tatatgagtt
     6121 tttagggctg tttacagatg gagatgacaa tttgtcgaag agttgggagc aatgcaaaat
     6181 ttagtagggg attataaaca gactgacaag gtatttaaag aacatgcaca agtatttcat
     6241 aatattgaag aatttttgaa tactgtgaga taggagttgc tacattgaga aaagtatgta
     6301 tttttccaat gtaaaagatt acgagtttga aaacgacacc ataatattat tttttaagaa
     6361 gatattatag agcttagg
//
LOCUS       DAYYBH010000113         6312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000113 DAYYBH010000000
VERSION     DAYYBH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.118"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACHAUG_002688"
     CDS             290..1135
                     /locus_tag="ACHAUG_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8481731.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACHAUG_002689"
     CDS             1416..2636
                     /locus_tag="ACHAUG_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8481732.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACHAUG_002690"
     CDS             2633..5353
                     /locus_tag="ACHAUG_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8481733.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACHAUG_002691"
     CDS             5337..6281
                     /locus_tag="ACHAUG_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8481734.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2355 a          977 c         1218 g         1762 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aa
//
LOCUS       DAYYBH010000114         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000114 DAYYBH010000000
VERSION     DAYYBH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.119"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHAUG_002692"
     CDS             725..811
                     /locus_tag="ACHAUG_002692"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8481735.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHAUG_002693"
     CDS             complement(932..1621)
                     /locus_tag="ACHAUG_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481736.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACHAUG_002694"
     CDS             complement(1628..1906)
                     /locus_tag="ACHAUG_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481737.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACHAUG_002695"
     CDS             complement(1866..2780)
                     /locus_tag="ACHAUG_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8481738.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHAUG_002696"
     CDS             complement(2762..3142)
                     /locus_tag="ACHAUG_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8481739.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHAUG_002697"
     CDS             complement(3592..3879)
                     /locus_tag="ACHAUG_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8481740.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHAUG_002698"
     CDS             complement(3899..4123)
                     /locus_tag="ACHAUG_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8481741.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHAUG_002699"
     CDS             complement(4578..4748)
                     /locus_tag="ACHAUG_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481742.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHAUG_002700"
     CDS             complement(4763..5296)
                     /locus_tag="ACHAUG_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8481743.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHAUG_002701"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHAUG_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8481744.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYBH010000115         5954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000115 DAYYBH010000000
VERSION     DAYYBH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.120"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHAUG_002702"
     CDS             <1..813
                     /locus_tag="ACHAUG_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8481745.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHAUG_002703"
     CDS             786..1229
                     /locus_tag="ACHAUG_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481746.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="ACHAUG_002704"
     CDS             2399..3367
                     /locus_tag="ACHAUG_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8481747.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="ACHAUG_002705"
     CDS             3384..3908
                     /locus_tag="ACHAUG_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8481748.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="ACHAUG_002706"
     CDS             4284..4565
                     /locus_tag="ACHAUG_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481749.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="ACHAUG_002707"
     CDS             4567..4983
                     /locus_tag="ACHAUG_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481750.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="ACHAUG_002708"
     CDS             5613..>5954
                     /locus_tag="ACHAUG_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM8481751.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2259 a         1017 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggcatagtgt acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DAYYBH010000116         5951 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000116 DAYYBH010000000
VERSION     DAYYBH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.121"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..684)
                     /locus_tag="ACHAUG_002709"
     CDS             complement(37..684)
                     /locus_tag="ACHAUG_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8481752.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(692..1378)
                     /locus_tag="ACHAUG_002710"
     CDS             complement(692..1378)
                     /locus_tag="ACHAUG_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8481753.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1384..2457)
                     /locus_tag="ACHAUG_002711"
     CDS             complement(1384..2457)
                     /locus_tag="ACHAUG_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8481754.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2679..3353
                     /locus_tag="ACHAUG_002712"
     CDS             2679..3353
                     /locus_tag="ACHAUG_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8481755.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3358..4698
                     /locus_tag="ACHAUG_002713"
     CDS             3358..4698
                     /locus_tag="ACHAUG_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8481756.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4847..5833)
                     /locus_tag="ACHAUG_002714"
     CDS             complement(4847..5833)
                     /locus_tag="ACHAUG_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8481757.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         1802 a         1129 c         1048 g         1972 t
ORIGIN      
        1 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
       61 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
      121 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
      181 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
      241 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
      301 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
      361 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
      421 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
      481 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
      541 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
      601 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
      661 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
      721 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
      781 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
      841 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
      901 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
      961 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
     1021 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
     1081 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
     1141 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
     1201 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
     1261 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
     1321 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
     1381 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
     1441 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
     1501 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
     1561 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
     1621 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
     1681 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
     1741 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
     1801 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
     1861 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
     1921 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
     1981 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
     2041 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
     2101 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
     2161 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
     2221 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
     2281 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
     2341 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
     2401 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
     2461 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
     2521 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
     2581 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
     2641 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
     2701 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
     2761 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
     2821 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
     2881 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
     2941 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
     3001 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
     3061 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
     3121 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
     3181 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
     3241 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
     3301 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
     3361 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
     3421 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
     3481 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
     3541 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
     3601 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
     3661 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
     3721 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
     3781 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
     3841 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
     3901 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
     3961 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
     4021 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
     4081 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
     4141 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
     4201 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
     4261 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
     4321 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
     4381 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
     4441 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
     4501 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
     4561 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
     4621 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
     4681 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
     4741 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
     4801 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
     4861 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
     4921 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
     4981 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
     5041 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
     5101 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
     5161 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
     5221 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
     5281 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
     5341 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
     5401 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
     5461 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
     5521 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
     5581 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
     5641 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
     5701 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
     5761 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
     5821 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttactt
     5881 ataaatttat tctataggag acgattgttt tctacaaata tttagattgg aatttgatta
     5941 aaacaattta a
//
LOCUS       DAYYBH010000117         5906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000117 DAYYBH010000000
VERSION     DAYYBH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.122"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..1129
                     /locus_tag="ACHAUG_002715"
     CDS             122..1129
                     /locus_tag="ACHAUG_002715"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8481758.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1141..2145
                     /gene="neuB"
                     /locus_tag="ACHAUG_002716"
     CDS             1141..2145
                     /gene="neuB"
                     /locus_tag="ACHAUG_002716"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8481759.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2142..3290
                     /gene="neuC"
                     /locus_tag="ACHAUG_002717"
     CDS             2142..3290
                     /gene="neuC"
                     /locus_tag="ACHAUG_002717"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8481760.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3263..3940
                     /locus_tag="ACHAUG_002718"
     CDS             3263..3940
                     /locus_tag="ACHAUG_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8481761.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3891..5072
                     /locus_tag="ACHAUG_002719"
     CDS             3891..5072
                     /locus_tag="ACHAUG_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8481762.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5085..>5906
                     /locus_tag="ACHAUG_002720"
     CDS             5085..>5906
                     /locus_tag="ACHAUG_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8481763.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVM"
BASE COUNT         2188 a          752 c         1143 g         1823 t
ORIGIN      
        1 aatcaatcaa atgttgattc gttacgtttt gacatcatta ttgataatta gtttagttat
       61 atatttttta aagattaaga atcgaattat agatgactat aattcatagg aggagatata
      121 agtgtttaaa ggaaaaacaa tattagtaac tggcggaact ggttccattg gaagtgaaat
      181 tgttcgtcag cttttaaaat atgaaccaaa agctatcaga atttatagta gaggtgaaga
      241 aaaacaattt tatatgcaac aagagctctc aaatcacact aatttgagat ttttaattgg
      301 tgatatcaga gactctcaaa ggattgatta tgcatgtcgt gatgtggata ttatttttca
      361 tgctgccgca atgaaacacg ttccagcaag tgaatataat cctatggaag ctgtaaaaac
      421 aaatgtgatg ggaactcaaa atgtaataga tgcagcgatc aaaaataatg taaaacattt
      481 tgtaggtatc agtacagata aagtagtgag tcctactaac accatgggtg cgacaaagtt
      541 attgagtgaa aaattaataa tagctgccaa tcagtataaa ggaaaaacag aaacaatatt
      601 ttcttgtgta aggtttggaa atgtcatggg gtcaagtgga tctgttattc ctctttttag
      661 taaacaaata aaaaacaata aagatattac tttgacagat gttgatatga cacgatttat
      721 gatgtcaata ccggatgctg caagattagt tctgtctgct gctatgaatt caaaatttgg
      781 agaaacatat gtcttaaaaa tgccggcaat caatatttta actttagctc aatcattaat
      841 aaaaatatat aatgatacac atgatgaaaa atatagaggt gaaataaaag ttgtaggtat
      901 caggccagga gaaaaattat atgaagagct aatgactaca gacgaaattg aacgctcata
      961 tgaaaatgat aaaatgtata tcattccacc acactatcat gctactgacg aactgtacaa
     1021 agattatgaa aaaattaaaa tcactgcata ctcttcagat gcaacagaat taatgagtgg
     1081 acaggaattt attagaatcg caaaagagaa taatgtcgtg acttggtaag gagttttatg
     1141 atgaaatcag aagtatatgt aattgcagaa attggtgtaa accataatgg aagtctagat
     1201 ttagctttag aaagtattga taaagctaaa gaatgtggag ctaacgcggt taaatttcaa
     1261 acatttaaaa cgaataagct agtttccaaa aaggctccaa tggcaaaata tcagaaggat
     1321 aatcttaaag aggacagttc tcaatttgaa atgctaaaaa aattagaatt atctcaatct
     1381 gattttatta gaataaaaga ttattgtata caaaataaaa ttgatttttt gtctactcct
     1441 tttgatgaag aaagtgcaga ctttctaaaa caaattggtg tacaaggatt taaaattggc
     1501 tcaggagatt taacaaattt accttttctt agaaaaatag atatgtatgg gttgccagtt
     1561 ttattatcta caggtatggc tgatattcat gaggtcagtg aggctgtaga ttgtttcata
     1621 gatagtccag ttactatttt acattgtact tcaaattatc cggctagttc tgaagatatt
     1681 aatttattgg cgattaacac attaaaagaa atgttcggga tacccgttgg gtactccgat
     1741 cattcattgg gatatgatgt tggtatttgt gctgttgcga tgggtgctaa ggtaattgag
     1801 aaacatttta ccttggataa ggaactacct ggtccagatc ataaagcttc tttagatcct
     1861 caggaatttg cagattttgt aaagcatatt agaaacactg aaatattttt aggtgatggt
     1921 gttaagcgag caatgccttc tgaacttagt acaaaagaag tatcaagaaa aagcctagtg
     1981 gtcactgaga atttaaatgt gggagatatt cttacagaga aatctcttac aataaaacgt
     2041 cctggaaatg gaatagctcc taagttttta tatcaatata ttggcaaaaa agttaaaaga
     2101 ccaataagtg cagatagtgt tctgcttgag gaagatgttg aatgaaaaaa atacttatag
     2161 taagtggaac acgagctgat tatggaatat ataaacctgt tgctgagaat ttactacaag
     2221 ataattggga tgtatcattt gttgtttctg gaatgcattt atcccataaa tatggattta
     2281 ctaaagaatt gatcttagct gacggattta gaattagtgg agaagttcaa agtttgtttt
     2341 tagagaacaa taagggaaac atggctcggt ctgtggcatt agagataaat ggtttcacaa
     2401 atatatttga acaaactagc cctgattttg tattattact aggggataga ggagaaatgt
     2461 tggcagctgc gatagcttgt ttgtatcttg gaatcaaaac agcacatatt cacggaggcg
     2521 aagtgtctgg tactgtagat gaatcaataa gacatgccat aacgaaaata gcatctgttc
     2581 attttgctgc tacggaaaaa agcaaagagc gtttaataaa aatgggggaa gattcttgga
     2641 gagtttttcc cgtcggagct cctagaatag atactatttt aaataataga ttacctagtt
     2701 ttagagatat aaaagaaaaa tatcatctaa aatttgaaaa gaaaaaatac gttttgcaag
     2761 tatttcatcc agttgtaacc gaactagaaa atatagaaaa acaaatttta gctttgatca
     2821 attcaatgga acaacaatct caaccaatta tttgcatttt acctaatagt gatgctggta
     2881 gcgaaattat cagaaaaatg taccagaaat atttaaaaac ggatattatt tatattacga
     2941 atttagagcc ggaggaatac ctgactatat taaaagagtg caaatttatg atcggaaatt
     3001 cttcttctgg gataattgaa gctgcatcat tcaaaatacc agtaataaat ttaggaacca
     3061 gacaaaatgg acgagaacaa tctaaaaata ttataaatgc aaatctaaat gaaaatgaaa
     3121 ttgaaactgc tttgaataaa atacaagatc ctcagtttat aaaaagcttg aaaaatgtta
     3181 agaacatcta cggtgacggc aacagttcta gaagaataac aaaaacattg aatcaaatat
     3241 tgaatgatgg ggaagatatt aagtggattc aaaaacaaat agcttattaa aatatactag
     3301 tgatccatac attattattg gggctggcgg acacgcaaaa gtcattattg aaaccttaag
     3361 attagaaaat aagttaatat atggattttt tgatgataag caaaataaaa tgcaagatct
     3421 gaaatatctt gggaaaataa acgaattgaa tagctgggcg gatagcaaaa aaaaaatcta
     3481 taaatttatt gtagctattg gtgactgtgt agtaagaaaa aagattataa aaaaattgaa
     3541 tatgccaagt gagcaatatg gaatagctgt tcatccaagt gctatagtgg caaaagaaat
     3601 ttcaattggt tatggaactg ttatttttgc gaatgctgta attaacccag atgctgtaat
     3661 tggagagcat gctataataa acacaggtag cattgttgag catgattgta gaattaataa
     3721 ctatgttcat ttatctccag gtgtatgctt agcgggtgga gtacatgtag gtgtaggaac
     3781 tcaaatagga attggttctc aatgtatcca gatgaagaaa attggatctt ggtgtatgat
     3841 aggcgcaggg agcactattg ttaaggatat tccctcacat tccttagctt atggcaatcc
     3901 agctaaaatt aagaaagaag gaattaattt tgaattctag aattttatta tcaagtcctc
     3961 atatgggagg aacagaagaa aaatatgttg ctaaggcttt taaagataat tggatagcgc
     4021 cattaggtga aaatgtggat aaatttgaag aaagtattac aaaatatact ggttctaagg
     4081 gcggagcttc tgcgttaagt tcaggtacag ctgccataca tcttgcctta agactgctta
     4141 atgttggaaa aaacgatatt gtctttgctc cttcatttac ttttatagct acagtaaatc
     4201 ctattttata tcaaggggct gtaccagtgt tcattgactc agaacctaat acatggaata
     4261 tgagccctgt tgcactagag agcgcattag aagaagctga aagaaacaac caattaccta
     4321 aggcaatcat cgtagtgaat ttatatggtc aaatggctga ttatgaacga attttagcta
     4381 ttgcaaacca atttaatgtt ccagtgatag aagatgcagc ggaatcgtta ggttctactt
     4441 ataagggaat ttcgtcaggc ttatttggga caattgggat ctattcattt aacggtaata
     4501 agatcatcac aacctcaggt ggaggagctc tagtgtctag tgataaaaaa tatgttgagc
     4561 attcaagatt tttagcaaca caagcaagag atcaagcacc gtattatcaa catagcgaaa
     4621 taggttataa ttaccgtcta agcaatattt ctgccggtat cggacgtgga caaatggaag
     4681 tattgaatga tagagtagct aaacgaagag aaattttcaa tcgatataag gaagcttttt
     4741 ctggaattga tagaattgaa atgatgccag aagtggatgg catggtgcca aatcgttggt
     4801 tgtctacttt aacgatcaat gattataatt ctattgatca agtagttaac gtaataaata
     4861 aactaaatga aaaaaacatt gaaacaagag ctttgtggaa accactacat ttgcaaccag
     4921 tatttcaagg ggtaaaaatg tattaccatg ataataatct tagtgtgtca gaaaagttat
     4981 ttaatagcgg cttatgctta ccttctggaa gtaatatgac agatagtgaa caagaatttg
     5041 ttattgatga gtttaaaaaa atattcaagt aggtgtgata ttttatgaac ataggaatta
     5101 ttggtttagg ttccatgggg aaaagaagac ttagactcat tcaagaacac tttaatgata
     5161 ttttactatg tggtgttgat actaatgagg cgagacgaaa ggaagttgaa aaggaatttg
     5221 gaataccaac ttataatagt ctaacagagg caaaaaattt gtttgaaata aattcagttt
     5281 ttgtttgtac atcccctata acccatgaat ttattataaa agaggcttta aatttagatt
     5341 gtaatatctt tacggaaatc aatttattaa atgaatatta tgatgaaata atcgaagtag
     5401 ctaaaaagaa agatagatta ctttatctat cttctacatt tttgaagaga aaagaaattc
     5461 aatatattaa gtctaaagtg acgcataata aaaaaatttc ttatcaatac catgtaggac
     5521 agtatttacc cgactggcat ccttgggaag attatcgcaa tttttttgtt tcaaacaaaa
     5581 aaactaatgg gtgtagagaa atattcgcta ttgaattacc ttggattata gatacatttg
     5641 ggaaagttaa atcgtttact gtaatgaccc agaaattatc caatcttgat attgattatt
     5701 cggacagttt cactcttatt attgagcatg aaactggtat agttggaact ttaagtgtag
     5761 atgttgtttc aagagtagct aaaagaaatt taactataat tggtgaaaag attttaatag
     5821 aatgggaagg taacccggat tctctatttt catggaatga agaaacttct agtatgacag
     5881 atattcaatt gtacgatgag gttatg
//
LOCUS       DAYYBH010000118         5880 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000118 DAYYBH010000000
VERSION     DAYYBH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.123"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..564)
                     /locus_tag="ACHAUG_002721"
     CDS             complement(106..564)
                     /locus_tag="ACHAUG_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8481764.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(577..1908)
                     /locus_tag="ACHAUG_002722"
     CDS             complement(577..1908)
                     /locus_tag="ACHAUG_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8481765.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1919..3361)
                     /locus_tag="ACHAUG_002723"
     CDS             complement(1919..3361)
                     /locus_tag="ACHAUG_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8481766.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3348..4112)
                     /locus_tag="ACHAUG_002724"
     CDS             complement(3348..4112)
                     /locus_tag="ACHAUG_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8481767.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4216..5091)
                     /locus_tag="ACHAUG_002725"
     CDS             complement(4216..5091)
                     /locus_tag="ACHAUG_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8481768.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5346..5430)
                     /locus_tag="ACHAUG_002726"
     tRNA            complement(5346..5430)
                     /locus_tag="ACHAUG_002726"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5394..5396),aa:Leu,seq:caa)
     gene            complement(5448..5519)
                     /locus_tag="ACHAUG_002727"
     tRNA            complement(5448..5519)
                     /locus_tag="ACHAUG_002727"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5485..5487),aa:Gln,seq:ttg)
     gene            complement(5529..5601)
                     /locus_tag="ACHAUG_002728"
     tRNA            complement(5529..5601)
                     /locus_tag="ACHAUG_002728"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5566..5568),aa:His,seq:gtg)
     gene            complement(5663..5747)
                     /locus_tag="ACHAUG_002729"
     tRNA            complement(5663..5747)
                     /locus_tag="ACHAUG_002729"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5711..5713),aa:Leu,seq:caa)
     gene            complement(5768..5838)
                     /locus_tag="ACHAUG_002730"
     tRNA            complement(5768..5838)
                     /locus_tag="ACHAUG_002730"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5804..5806),aa:Cys,seq:gca)
BASE COUNT         1797 a         1253 c          894 g         1936 t
ORIGIN      
        1 acaaaaatca aaatgattaa atctagtgat tgaaacatat catttcaaaa aaattttgta
       61 aattcaccca taaatatcac cctatttaac ttttttcatc gtacactaaa attcaaaaca
      121 actatcttta atctttactt cttggttttg ttttaataaa tttccttcaa aaatgcctgg
      181 agaaactaca atgatcaccg tatctttttg tgcttgctcc aacttcttca aatctataat
      241 agctaacagt gtcccttctt ttacctcctg gttactttca acccgtatct gaaacggact
      301 tccttcgagt tcaactgtat ctaaacccat atggatcagt atttttctgc cagattcact
      361 ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg attattcctt taactggcgc
      421 atggatatat ccatcatgat tcgttatagc gtatccaact cctatcattt ttgaagaaaa
      481 gacctcgtct tttactgctg ttaatggaat aatttgacca ctagctacag cttttagaat
      541 gtttcttgtt ttaaagaaat gcacttttta tcacctctac tttaatgctg gccaatattt
      601 tttgtttact ttgatcattt catctagtat tttttttgcg actctggcat ttggtacggt
      661 ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt aagcatctac
      721 tagtaacttc tctactgcaa cttgtgcctc catcaatcct ttatgaaaat cactgattgg
      781 ttttcttagt gtaattggct ccacaccagt tgcaccaaca tatgccggta tttcaacaac
      841 tgcatctgac cgtagattcg gaatcgcccc ttgattagga acgattagca taaagcgacg
      901 acgatcatca ttcaacaagg aaatagctat atcgacgata tattgtccat gctccccaaa
      961 attaaagttc aatacttctt ccacaatacc gcggcgtatt ttatcagcca tttctttggt
     1021 atttttctct cgtccatcca tgaccatatt agcacgtgta tactctttgt cagtatgctc
     1081 aacgaccata tcaggatata agtaatactc taagtaatta ttcgggatat tacttggaaa
     1141 atttttcgtg atgactgaca tcatttgaaa ggttttttgc caagttttat ctcctatatt
     1201 aaaatcggcg acttgcattt cttttgttat tagtttttca ataatctctg gcataatttc
     1261 tcgttgcaat tctttatcat aaatcgatgt ataccagcca aagtggttta aaccataata
     1321 cgtaacgatc caattcttac gatcataacc gtaattcact gcaatcgttt cttcaattga
     1381 aatagtcata tcacaggcat taatcatttt tacattcgga tatttacgac gaacagcttc
     1441 agaaacgatc gactctggat ttgtataatt caaaatccat gctttggggg catagtcttg
     1501 tatatgatcg acaatttcca ataaaccttt gattgagcgc atcccataag caaatcctcc
     1561 gagtccacac gtttcttgtc ctactaatcc atgtttaagt ggaatctttt cgtccttttc
     1621 tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc
     1681 aagctttgga tcatccgttg cacgaatgcg aatatttgaa aaaccttttc ttttcagcat
     1741 aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat
     1801 ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata
     1861 cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct
     1921 attcttctaa taatttattg aattctgcca taacataagt cacttctaag ccgatgataa
     1981 tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct
     2041 gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta caactataga
     2101 catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat
     2161 aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg
     2221 ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa
     2281 cgtaatacaa agcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga
     2341 ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat
     2401 tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca
     2461 taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag
     2521 ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt
     2581 tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa aaaactgata
     2641 aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt
     2701 ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt
     2761 gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgctc
     2821 ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa
     2881 agcccaaaaa aacagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt
     2941 cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc
     3001 atgccgctac taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca
     3061 taatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc
     3121 ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt
     3181 ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata tcgtcagctg
     3241 ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc
     3301 caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca
     3361 tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt
     3421 aaaaattcga cttggtgggt acgaggatac acaaaacttg aaccatttaa caattcctga
     3481 tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt
     3541 aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct
     3601 cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca
     3661 aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctgattt
     3721 tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga
     3781 taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat
     3841 ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt
     3901 ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt
     3961 tcctttctta gtttcacagc atgctcgaaa cccggcatat ttgccatcaa tgtagttgat
     4021 gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca
     4081 aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa
     4141 taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat
     4201 agttacgccc ttattttaat agattcttgc tagatgaatt gccaaataga tgatttcatt
     4261 ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca
     4321 atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc
     4381 ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt
     4441 cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt
     4501 cgtcatttct actattttta agctctttaa actattaggc tctcctagct cagcattaat
     4561 gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt
     4621 gattcgctcg accacttcta ctgctatatc atattcattt ggataaagga atctcatttc
     4681 tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat
     4741 atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat
     4801 cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc
     4861 actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac
     4921 atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc
     4981 tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt
     5041 taattcacct cctctatatg ataaaacaaa acaacttgca gactccttca tacatatttt
     5101 tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg
     5161 tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa
     5221 gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag
     5281 gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat
     5341 aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc
     5401 cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg
     5461 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     5521 gaataaaagg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt
     5581 taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca ataaaaaact
     5641 aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg
     5701 gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt
     5761 agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctctgcctta
     5821 ccacttggct ataccgccat tatactatta tcaatctata aaataaagaa taaattatag
//
LOCUS       DAYYBH010000119         5689 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000119 DAYYBH010000000
VERSION     DAYYBH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.124"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACHAUG_002731"
     CDS             complement(488..1297)
                     /locus_tag="ACHAUG_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8481769.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACHAUG_002732"
     CDS             complement(1297..2043)
                     /locus_tag="ACHAUG_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8481770.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACHAUG_002733"
     CDS             complement(2061..2528)
                     /locus_tag="ACHAUG_002733"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8481771.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACHAUG_002734"
     CDS             complement(2528..2938)
                     /locus_tag="ACHAUG_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8481772.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACHAUG_002735"
     CDS             complement(2949..3962)
                     /locus_tag="ACHAUG_002735"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8481773.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACHAUG_002736"
     CDS             4179..4913
                     /locus_tag="ACHAUG_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8481774.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACHAUG_002737"
     CDS             4897..5607
                     /locus_tag="ACHAUG_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8481775.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAYYBH010000120         5551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000120 DAYYBH010000000
VERSION     DAYYBH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.125"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHAUG_002738"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHAUG_002738"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFM8481776.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHAUG_002739"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHAUG_002739"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFM8481777.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHAUG_002740"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHAUG_002740"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFM8481778.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACHAUG_002741"
     CDS             complement(4028..5044)
                     /locus_tag="ACHAUG_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8481779.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5298)
                     /locus_tag="ACHAUG_002742"
     CDS             complement(5146..5298)
                     /locus_tag="ACHAUG_002742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481780.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
     gene            complement(5315..>5551)
                     /locus_tag="ACHAUG_002743"
     CDS             complement(5315..>5551)
                     /locus_tag="ACHAUG_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFM8481781.1"
                     /translation="CSCCGEYYEFVGMSVRKNGRLVIKYAKDNHAKRFMREENRKIDQ
                     KEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1461 a         1377 c          973 g         1740 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac a
//
LOCUS       DAYYBH010000121         5481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000121 DAYYBH010000000
VERSION     DAYYBH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.126"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACHAUG_002744"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACHAUG_002744"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACHAUG_002745"
     CDS             complement(580..1353)
                     /locus_tag="ACHAUG_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8481782.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACHAUG_002746"
     CDS             1497..1847
                     /locus_tag="ACHAUG_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8481783.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACHAUG_002747"
     CDS             1828..2544
                     /locus_tag="ACHAUG_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8481784.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACHAUG_002748"
     CDS             2544..3992
                     /locus_tag="ACHAUG_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8481785.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACHAUG_002749"
     CDS             complement(4062..4571)
                     /locus_tag="ACHAUG_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481786.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACHAUG_002750"
     CDS             complement(4561..5286)
                     /locus_tag="ACHAUG_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481787.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAYYBH010000122         5312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000122 DAYYBH010000000
VERSION     DAYYBH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.127"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACHAUG_002751"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACHAUG_002751"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8481788.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="ACHAUG_002752"
     CDS             complement(1298..2134)
                     /locus_tag="ACHAUG_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481789.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="ACHAUG_002753"
     CDS             complement(2318..3535)
                     /locus_tag="ACHAUG_002753"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8481790.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3684..3845)
                     /locus_tag="ACHAUG_002754"
     CDS             complement(3684..3845)
                     /locus_tag="ACHAUG_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481791.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3918..4001
                     /locus_tag="ACHAUG_002755"
     tRNA            3918..4001
                     /locus_tag="ACHAUG_002755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..5312)
                     /locus_tag="ACHAUG_002756"
                     /pseudo
     CDS             complement(4241..5312)
                     /locus_tag="ACHAUG_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1739 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac at
//
LOCUS       DAYYBH010000123         5189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000123 DAYYBH010000000
VERSION     DAYYBH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.128"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACHAUG_002757"
     CDS             137..748
                     /locus_tag="ACHAUG_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8481792.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACHAUG_002758"
     CDS             821..1750
                     /locus_tag="ACHAUG_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFM8481793.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACHAUG_002759"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACHAUG_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACHAUG_002760"
     CDS             complement(2693..3895)
                     /locus_tag="ACHAUG_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8481794.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACHAUG_002761"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACHAUG_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5189)
                     /locus_tag="ACHAUG_002762"
     CDS             complement(4706..>5189)
                     /locus_tag="ACHAUG_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8481795.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAYYBH010000124         5176 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000124 DAYYBH010000000
VERSION     DAYYBH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.129"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACHAUG_002763"
     CDS             complement(136..1098)
                     /locus_tag="ACHAUG_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8481796.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACHAUG_002764"
     CDS             complement(1100..2539)
                     /locus_tag="ACHAUG_002764"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8481797.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACHAUG_002765"
     CDS             2724..4670
                     /locus_tag="ACHAUG_002765"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8481798.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="ACHAUG_002766"
     CDS             4934..>5176
                     /locus_tag="ACHAUG_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8481799.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DAYYBH010000125         5153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000125 DAYYBH010000000
VERSION     DAYYBH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.130"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..561)
                     /locus_tag="ACHAUG_002767"
     CDS             complement(52..561)
                     /locus_tag="ACHAUG_002767"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8481800.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(673..1671)
                     /locus_tag="ACHAUG_002768"
     CDS             complement(673..1671)
                     /locus_tag="ACHAUG_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8481801.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1872..2456
                     /locus_tag="ACHAUG_002769"
     CDS             1872..2456
                     /locus_tag="ACHAUG_002769"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8481802.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(2533..3708)
                     /locus_tag="ACHAUG_002770"
     CDS             complement(2533..3708)
                     /locus_tag="ACHAUG_002770"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8481803.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            3790..4170
                     /locus_tag="ACHAUG_002771"
     CDS             3790..4170
                     /locus_tag="ACHAUG_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8481804.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            4244..4606
                     /locus_tag="ACHAUG_002772"
     CDS             4244..4606
                     /locus_tag="ACHAUG_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481805.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1684 a          892 c          958 g         1619 t
ORIGIN      
        1 aaaaaagaaa gaactcgtct aaatagacgt tttctttctt tttttgttga attattgagt
       61 aaggcgactt cttttttctg gttctttgtg tgctaatcga ctggcagcag cagcggcgat
      121 cgctccgacg atatcatcta agaaggtatg gacatgaacg ccatcgtggc tgtttagttc
      181 ttttaaaata ccaggtttca ccttgtcgat ataaccataa ttcgtaaagc cgatcgtacc
      241 gtaaacattc acgattgaca aggctaggat ctcatcgata ccgtaaaggc cttcgtcatc
      301 ttcgataata tgttgcaacg gactcatcaa ttttttcttt tctgctaata tatctaattg
      361 gattccagtg ataatggcgt tatgtacttc acgctttgtt agcacgctgt tgacactttc
      421 agtacaatcc gccaaagtca aatcagaaat atagtctttt tgtaaaaaca tgacaagttg
      481 tgcgatatct tctacagtta cgccacgttc cttcaatagt tctcttgcct tttcttctaa
      541 agtctcagtt ttgacgacca tattattgct ccccctttat tttttctttc tctatttcat
      601 gttaacggaa catgaaaaaa atgcaagaaa ctgctttcgg gtttaacttg aaatgcaaag
      661 agatataaaa aatcaactag gacgaagatt tttatcgtat aagcttgtgc tgtgaccagg
      721 ctgagttcgt tcctttggat taatatagtg taaagcgttg ttcactgcag taggtgcttc
      781 accgagccca gtagcaatca gtttcacttt tccttcatag gtacagatgt caccagctgc
      841 ataaacgcct ggaatagaag tagacatatc tgattttaca gtaatggcat ttctggtact
      901 atcaagtccc caagaagaaa gatgttctaa attagaagag aatccataat tgacgatcag
      961 agaatcaatc atcaaatcaa tcgtctcgtc acttttcact ttttttaacc ggatatctgt
     1021 cagttcccca ttattgcctg ataatccatc gatgagatag ggagtaagca agttgacaga
     1081 tgaagatttc aagcgtgata cactatgttc gtgcgcacga aattccgggc gacgatgaat
     1141 caagtaaact tcgcttgcaa taggctctaa catcaacgtc caatcgatag cagaatcacc
     1201 acctccagca atagcgactt tttttccagc atacttcatc aaatcattca cgaaataatc
     1261 caatccgtgg ttttcaaatg attcagcatt atctaaattc aattttcttg gctggaatga
     1321 accatttcct aaagctaaaa tgactgcttt ggaataatgg atgcctttat ttgtagtaat
     1381 ctcgataact tcgtcttcac gagtaagcga gagaacttct tcttttaaat ggaatgtatg
     1441 gttaaaagta gttaattgtt tttctaattg atcgattaat tcacttgctt ttattgctgg
     1501 atacccagga atatcgtaaa tatatttttc gggatacaaa gttgctaatt gaccaccaag
     1561 ttgaggtaga ctgtcaatga tttttgtttt agcttggcgc attcctgcgt aaaaagcggc
     1621 aaacatccct acaggacctg caccgatgat tgtaatgtca tagatttcca taaaaattcc
     1681 tcccttttca cactatagca gaatttaatt gagaatgctt ctcaaaaatg tttgaattcg
     1741 aaatcgaaac ggtaagttat agaatgtatc tagtgatgaa aaataaaaga aaacaaatct
     1801 agtattttta cgagaggtta ggtattatgt tattattatt atgaagtatg caaatattag
     1861 gaggaaacaa tatggcattc ccacaactag atttaaaaag tgaaaaagga ccaaaagctg
     1921 ttatcaaaac aaaccgtggt gacattactg ttcaattatt ccctgaacta gcaccgaaga
     1981 cggttcaaaa tttcattgaa ctttccaaaa aaggttatta tgatggcgtg atttttcacc
     2041 gcgttattcc tgatttcatg atccaaggcg gcgatccaac tggaacagga atgggtggcg
     2101 aaagtattta tggcgaaagt tttgaagatg aattcagtcg tgaactgttc aaccttcgcg
     2161 gagctttatc tatggcaaat tcaggtccaa acacaaacgg aagccaattc tttattgtaa
     2221 ataacacaaa tgtgccagct aatatgcttg gacaactaga aggtgctgga ttcccatcag
     2281 aaatcattga agcgtataaa ggtggcggaa ctccttggct agatttccga catacagtct
     2341 ttggacatgt actagaaggg atggacacag tagatgaaat cgcaaatgta caacgtggtc
     2401 cacaagaccg acctgtacat gatgtagtga ttgaaacaat tgaaatttca gaatagtttg
     2461 gtatggtacg gagtaattat gaaaaaggac gaaacctagt aaggtctcgt ccttttattt
     2521 tgaataaatg atttaatcga ttgtctctaa agcatacttg attcgattaa gtccttccag
     2581 taaagtttct tctggacaag caatattcaa gcgcatatgc ccatgaccgg aaggaccaaa
     2641 cgtgatgcca ggatttaata cgacttttcc tttccgaaca agagtgttct ccaattcttg
     2701 gtcgtctgaa atataggcgg taaaatctaa ccacattaag taagttcctt cagggttcat
     2761 tattctcact ttcggaagat actgttcaaa aaactgatta actgtttgaa catttttttc
     2821 tagataagga atcagttggc ttaaccattc tccacccgtc tcataagccg cttgggttcc
     2881 aatcagtcca aaagtgttga tttcgtgctg ttggttcatc actaaatgtt tttcaaaatc
     2941 agctttcatc tctggatttt tgataaatac cattgaattt ttgattgcag ctagattgaa
     3001 agtcttggtt gcggaagtaa aagcaactag tagatcatct aaattaggat caatcgttag
     3061 catggaagta aatgtatgat cgaataaagt taaatcttga tggatctcgt cactaaacag
     3121 aatcacttga tgttttaaac acaattcgct taactgtttc aattcttctt tgctccaaac
     3181 acgccctcca ggattgtgag gattgcataa aatcattgct tttacattgt tttcttcgat
     3241 ttttttctcc atgtcagaca agtccatgat gaaatgatta tttttttcta gcaaagagct
     3301 tcgaacgatg tgacgttgat ttgtttctat aatagaggaa aatggaggat aaactgggtc
     3361 atgaatcaaa atagaatcgc ctggttttgt aaaagtttgt actgctgcag ctaaactggt
     3421 taacacacca cttgtaaaaa cgatattttc tttcgtcaac tccacagcat ggtgttggcg
     3481 ttcccaatga atgatagcct gatatagttt atcagacaga gtggcataac caaagattcc
     3541 atgtttgatg tattcactaa acgctttagc taccccaccg ggagacaaga agtccatatc
     3601 tgctacccac aaaggaagta agtcatccat ttgatatgtc tcagcaattg cgtcccattt
     3661 tacactttct gtatcttttc ttgaaatctg tttatcaaat tcgatcatct taagacctcc
     3721 agtcattttc gtcgcttctt tttttgagct atttacagta taatgataaa tgcgaagaga
     3781 gggaagtaga tgacttataa aattggacaa ataattgaag gaaaagtgac agggatacag
     3841 ccttatggcg cattcgtttc tcttgataac gagacgcaag gacttattca tgtttcagaa
     3901 gtacaatccg gttatacgaa aaatatccat tcattgttaa aagtcggaca gccagtaaca
     3961 gttcagatca tcgacattga tgaatattcc aaaaaaatca gtttatcttt aagaacgtta
     4021 gaaaaaacaa atccagctgt tccatatcgc agaaaaaggt atttcacgaa taaaaatcgt
     4081 aagatcggat ttaccactat tgcggagcag ttacctattt ggatagaaga agcattatct
     4141 gaactagaac ggaaagaaaa taagaaataa aaagtgtatt ttttctgaaa atgtgctaga
     4201 atttttatgt aagccataga acaagttgat cgaggtggaa attttggcaa gcaaaagagt
     4261 tgctggtaca attatgttgc atttggagga tggtactaaa aaatttttgg tccattcaat
     4321 tgatgacaag ttggaatttg cgttagcgga actttctgaa ggaaagactg gtttagcgaa
     4381 tatacttaat ttcttaaaag aaatagttca tatcgatgtt agcaagatta gtttgatgga
     4441 attgacaaat acacatatta aagaggaaaa tgttccgttg tttgtctttg aaactgaaca
     4501 aaagaatcaa agagaagaat taggcgaagg atatatttgg gaagaaccag gtcaaatgcg
     4561 ctctgttctt ggaacttacg aagtagaggg agtgcctttc ttctaaaata cagattaagc
     4621 gaacaaagaa gtttgaattt ttttcaaact tcttttttta ttgtttggtc caaaaaatac
     4681 aatgaaatgt tctgctaaag atagtttttt agtgcatttt attttcaaaa tatgttgaaa
     4741 acggttgtat aacgaataat aataattgat cgaaaaatca tttcacaaag taatgcttaa
     4801 tttatagttg accaaggaag aaatacatga taaatttaac agcgttgatt ctgaagtgga
     4861 tgttatatca catttctaaa aaatttaaaa aatagttatt gacatgttac taacgacatg
     4921 ataagataac tgagtcgtta aaaaagaacc acttgcgaag cttgattcta tcgaaaaaaa
     4981 cttcaaaaaa gttcttgaca aataacaaac gataagttat gataaatgag ttgctgaaag
     5041 atttcgaaaa acgaaatcga aaaaaacttt taaaaaagag ttgacaatcg cttgtctctc
     5101 tgatatgata taaaagttgc tacggcagcc aaaaacgaat cgggaaacac tcc
//
LOCUS       DAYYBH010000126         5146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000126 DAYYBH010000000
VERSION     DAYYBH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.131"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..497)
                     /locus_tag="ACHAUG_002773"
     CDS             complement(<1..497)
                     /locus_tag="ACHAUG_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8481806.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            complement(605..907)
                     /locus_tag="ACHAUG_002774"
                     /pseudo
     CDS             complement(605..907)
                     /locus_tag="ACHAUG_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            1000..1349
                     /locus_tag="ACHAUG_002775"
                     /pseudo
     CDS             1000..1349
                     /locus_tag="ACHAUG_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1621..1911)
                     /locus_tag="ACHAUG_002776"
     CDS             complement(1621..1911)
                     /locus_tag="ACHAUG_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481807.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            2313..2549
                     /locus_tag="ACHAUG_002777"
     CDS             2313..2549
                     /locus_tag="ACHAUG_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481808.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            2556..4508
                     /locus_tag="ACHAUG_002778"
     CDS             2556..4508
                     /locus_tag="ACHAUG_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8481809.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4580..5077)
                     /gene="dfrG"
                     /locus_tag="ACHAUG_002779"
     CDS             complement(4580..5077)
                     /gene="dfrG"
                     /locus_tag="ACHAUG_002779"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8481810.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1726 a          897 c          856 g         1667 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctcaacata cttgcaaagt aatactacaa ttcctaacat aatcgtagaa tttaataatt
      661 tccatagcat aaagtgttta gaaaagaaca ttaagaatag ttctataata attcttgaac
      721 tccatgtctt ataatgttct atcgaagcac ccaaatagcc aagttcatct aatctacttg
      781 cataaactac gtcatccgct ctcatagtcg gcatatacat attaaagagt aaaaagatac
      841 cccaaatccc tattagtaac aagatcagtt ctttgttatt cgttattttt ctattatgtt
      901 tgttcatttt tatactcctt cttatatttc ctaatagagt ataacatagc tttaagcatt
      961 acttaaagat aacttaaaag ttatctttta aatacttaat tgttaaatac cttatttgat
     1021 gcaaaagaaa tctacgatag tcaacaaaaa aattttcatt atatccagaa aaatttttct
     1081 tagttaagga cctatggatt gcagtaatgc agaattcgtc gaataaactg ccaaagacat
     1141 acaatcatat tgcttttaaa attgacgaac aagatataga ttcgtttgtc tcaaaaatcc
     1201 aaatgttagg tttaactgtc gaaccaggac gttctcgtgt gaaaggcgag gcttcttcaa
     1261 tttactttta cgactatgat aatcacctct tcgagttaca taccggtact ctccaggaac
     1321 gtttaaaatc ctacaattcc actacctgag actagtaatg actagtctct ttttatggcc
     1381 tctgatcccc ccattgagcg aatactaata gggcttcgaa ttgatctgtt caatctttaa
     1441 gcaatatccc tcaaatgagt ttggaaagtt tagtgattca tcacaaatac ttaaatacat
     1501 gtagttatat atagattgag gttcttgtga gcgtatgctc gtgtgggttc aaatcccatc
     1561 gtctgcatcc atctttccaa aacaagcggc taggacattt ttgtctcagc cgcttgtttt
     1621 ctagtttatt ctttcatctt gtcggacctt ttttcttggg tatagacttt tctcatattc
     1681 taccacaatt ttgggtttcc ctaacacatg ataggatctc acaacaaatt gctttctatt
     1741 attataataa cctgtaacga ctaacttcat cccttcgtct acttcagcta ggaagttcaa
     1801 actatgggaa gcaatcaaac aattgacccc atttaaactg aaacggatca acggtctggc
     1861 actaacttgc aaagtacgga tcttatctac tgttccatat tttttctcca taaccttcac
     1921 ctcaatacca ttatacgaac atacattccc tttgtaaaga agaaactctg ttttatcttt
     1981 ttgacttttt gtctattacc tttcttaaaa tcatattggc acttcctgcg aagatgaagg
     2041 acatgtacta aaaaaaagag gcagcagtat agatttaagt agttcagttt tggagtacaa
     2101 aaagtgaaaa tgtaaaccta aatgagtatc aaattgcaac tgaatttgat actcgttttt
     2161 tattgttaaa tcaacgtttg ttgttaattt acaccacaaa atgtaagtta aaatgtatat
     2221 aaaaatgcga acgtgaaata attattgtag gaatgcaaat tggtgtgtta aaatggttac
     2281 aatttactaa gcaaaaaggg gtgcattttc ctatgcaatt tcttgagcca aaaaataaga
     2341 acgctaagtc tgtggattgg gaaatatcag agcaagtaag ggtgatagtt aagcaatacg
     2401 ctgaatatgc agagcgtact gaaagcgaag cagtggatga attcttattg aacattttag
     2461 acgataagaa gtttattgaa tggattgcca acaagcgaag taataagcgg attgttgaaa
     2521 aaatgggtat caaagataga gtgggttgat agacaatgac caagcttaaa agattagcca
     2581 cgaaggaaga tgaaattgta gatgttaaaa ttccgatttc aaacgaagaa ttaaaagata
     2641 gggctaaaca atatgattta ttaactccaa aacgatttgc aacaaggtat aacaaaatgt
     2701 tgttcttgcc aacgtctttt aaatggaacg gttctgaata tcccattcaa tataattact
     2761 gcatcaatcc gttttgttgt aattttggga aagaacagca caaattcaaa gatgtaaaag
     2821 ggaaaccaag tcgatataaa atgactggta gttccaaaga taaaggtcat aaaggtatgt
     2881 actgcaatga caatcctatt ggcagaggtg tatcgcaaaa ctgtacagta acccctttat
     2941 ctaattggtc tgttgttgaa gaaattaaac gccttataga aatcaatagc atccaagatg
     3001 ttgagccaga ttatcaattt cataaagaag gctgctcgga agaagaatca accccattca
     3061 atgagccgaa gcaattttac aaaaggggga aaagtagggg caaatctcaa cgctatcaat
     3121 gtaaggcgtg taagaagttc actaatgttc taccaaaaag agaagaaacc actacttatc
     3181 accaacaaaa gaacaccatt cttccaatgt tcgctaagat ggttgttgga agggtttcag
     3241 tcagtcgtac ttgtgatatt ttaggaattg gagttggaac ctactatcat aagctggagt
     3301 ggctgtatag gcgttgtttg gagtttttag aacgatatga gacacaaccc cttcaaacaa
     3361 agaaatttaa cgagatgtgg cttaatacgg acaagatgca ctactaccta aataacgtcc
     3421 gtaagaaagg acaaggttca aagaaatata cggggtttga agaccttaat atgcaaacgt
     3481 atatagttgt atctgctgag gtcttatcaa gatatgtgtt ccgttctgat gttgcctatg
     3541 attggaatat ttcaatggat gaattaaatg aagatacaag aaaattcaaa gaagaccatc
     3601 tcaatacttt tagtcgtaaa aatgataggt tagattggtc ttattatcca caagaaccat
     3661 ccgctaacga tagcgaaaat aggaatgctt atcttcacga attagggaaa attactaata
     3721 ggagtagatt tgtcgatggt ttaaatgttg atgctcctta tactacaact gctcactatt
     3781 ggttaattaa acaaatggtt aatgccgacg aatggcgaat gattagtgat gatgacttct
     3841 ctattagaaa cgccttttat agagtattca cgaaagaatt aaggttgagc gatgcacatc
     3901 attttatttg ccaagttaac aagacgaaaa gcagaaaaca atgcttaaaa gaattcggac
     3961 aagcaaaagc agaactgctc gattggggag atatcagagg attcaaaaca aagtttctac
     4021 ggactttggc atctcattat ctaactgaat tactaacaag tcatcaattc cacgaagagg
     4081 ctattagtaa agatggagaa agatatcgca aatatgccga taaccctatc aaacatccat
     4141 tagccactaa ggataaagga ttttattcag ttgattgtag aacggattta tctgctctgg
     4201 aaccaaacga aatcgcaaag atgctattaa atgttaatga ccattcgacc aatagcttta
     4261 ttcaacagat aagacgttat atatcatctc ttgaaagacc attaacaaca gctcgtggtg
     4321 ataagaagag ctatatttac gctaacttca atccgaagta tgctcaattt gcgattacaa
     4381 ttctaagaac ttactacaac ttttgtcgcc cttttaaatc agcggataaa aaggttttga
     4441 cacctgctca aagattaggg attacagata aacaatttga ttggaaagat ataatttatt
     4501 tcaaatgaca aaaagagctt ggagtaatcc caagctcttg tgctttatct ttactgttct
     4561 tcttcaatta acgcacccgt taactcaata agttttttct ttcatataca tgaaaatagt
     4621 agttatacgg atttttatca ttctttatcc ctttttgggc aaatacctca ttccattcct
     4681 cataattcac ttctggaaaa aaagtatctc cttcgaattc atgatgtatt tttgtgatgt
     4741 acattttctc aacataaggg aaaaacaaat tataaatctg ttctcctccg aaaataaaaa
     4801 tttcttcttc gtttttacat aactcaaaaa catcttctat tgaatgaaca atttcacaac
     4861 cattaaaggt aaacccctta tctctcgtca gaataatatt tcttctgtca ggtaaggctc
     4921 ttccgattga ttcaaggttc ttcctaccta atattatcgg atgtcccttt gtagtatttt
     4981 taacatattc ccagtccttg ggaatcctcc aaggaatgtc attctctttg ccaatcactc
     5041 tattcttatc catcgcagca atcaaagaaa ctttcattta taacacagcc tttttaacta
     5101 atttaccatc taatttctta tttctatcaa aaaacctttt ctcatt
//
LOCUS       DAYYBH010000127         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000127 DAYYBH010000000
VERSION     DAYYBH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.132"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHAUG_002780"
     CDS             85..972
                     /locus_tag="ACHAUG_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8481811.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHAUG_002781"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHAUG_002781"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8481812.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHAUG_002782"
     CDS             2274..2720
                     /locus_tag="ACHAUG_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8481813.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHAUG_002783"
     CDS             2993..4345
                     /locus_tag="ACHAUG_002783"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8481814.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHAUG_002784"
     CDS             4516..4803
                     /locus_tag="ACHAUG_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8481815.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAYYBH010000128         4757 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000128 DAYYBH010000000
VERSION     DAYYBH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.134"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACHAUG_002785"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACHAUG_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM8481816.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACHAUG_002786"
     CDS             724..1878
                     /locus_tag="ACHAUG_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM8481817.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACHAUG_002787"
     CDS             2093..3061
                     /locus_tag="ACHAUG_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM8481818.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACHAUG_002788"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACHAUG_002788"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM8481819.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACHAUG_002789"
     CDS             4091..4699
                     /locus_tag="ACHAUG_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFM8481820.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DAYYBH010000129         4572 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000129 DAYYBH010000000
VERSION     DAYYBH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.136"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..873
                     /locus_tag="ACHAUG_002790"
     CDS             73..873
                     /locus_tag="ACHAUG_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFM8481821.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            889..1767
                     /locus_tag="ACHAUG_002791"
     CDS             889..1767
                     /locus_tag="ACHAUG_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFM8481822.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1780..2760
                     /locus_tag="ACHAUG_002792"
     CDS             1780..2760
                     /locus_tag="ACHAUG_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8481823.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            3031..3123
                     /locus_tag="ACHAUG_002793"
     CDS             3031..3123
                     /locus_tag="ACHAUG_002793"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8481824.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
     gene            3501..>4572
                     /locus_tag="ACHAUG_002794"
     CDS             3501..>4572
                     /locus_tag="ACHAUG_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8481825.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGE"
BASE COUNT         1589 a          741 c          973 g         1269 t
ORIGIN      
        1 cttgacagat aatcaaaaag aatttatagt tgttaccgta gtagctacta aaaataaagt
       61 gaggattaat caatggaaac gattattcaa atcatgaaaa aatatgattt ttcagagatg
      121 gagacaagag tgtataccac actattagaa aaaggtaatc ttacaggata tgaagtaagt
      181 aaaatctcag gtgtgccacg ctcgaaagta tataatattt tggaaaaact attaaaaaag
      241 aatttgattg tcgttaataa atcagaacct aaactttatc atgccatctc ggcaaatgag
      301 tttttagaga aattagaaaa aagtgttaaa aatgatcttt cttttcttac caagaatcta
      361 gggatgatca aagaaaaaga tgaagaagaa atgctgtgga aagttgatgg tatcgagtat
      421 gttctggata aagtagagca tttggtgaaa aacgcaaaag aaagtttgct cattcaagtt
      481 tggcatgaga atttcacgga ttcactacta aaggcattgc agcaagctga aaaaagagtt
      541 gataagtttg tactgatttt attcagttct acccatgaat atgatcttcc cttagaaaag
      601 tattatattc atggttttga aacagacaaa cttgctgact ttggagcaag atggatcaac
      661 atcgtcgccg atgagcaaga agtcgtgttt ggtaccatta acgaagaact gcaatcaacc
      721 gatgtcactt ggacaaaaaa ccacgccatg gtcaatctag caaaagaata tgtcaaacat
      781 gatgcatata cactaaaagt gattgcagaa tctcctgaag aacttaaagc aaaatatggt
      841 gagggctttg agggaatcag aaagatttac tagtaggagg aattttttat gattcggaat
      901 attttgctga cactaattgt cgcaatcaat ctatatttta ttatttactt tctaagagac
      961 cttttcaaaa acaaacaaaa tttcaaagaa gaaccagggg atatgcgtct gttgcccttc
     1021 acgtcattta tcatcttctt tctttcaaca tttggcgttt ccgactttgc tatcggaacc
     1081 gtcctttatc caaagttgaa atgggtcagc atgaaaaaac tacccggaac gttgaatacc
     1141 caatgtgtag taccggtagc cgtcatggca ttgtcttaca tcactgccat caacgtaggg
     1201 atcaagacac tagctgtttg tattatctgc caaattatcg gtgcatattt aggaccaaga
     1261 tttgtcgtga agttaccaga gaaaacgatc aaactgtttg tcggcatagg attggtcatt
     1321 gctgcattgt tgatttttgc aggacaaatg aactggattc catcaaacgg aacagccagt
     1381 gaattgtatg gcggaaaatt aatcttggca ggatttttac tctttgtcta tggtgcattg
     1441 aacaatatcg gcattggttc ttatgcgtta acgatggtaa ctgtctactt gctaggcttg
     1501 aatccagttg cagcctttcc tattatgatg ggggcttgta ccttctcagt accgatcggc
     1561 agtgttcaat tcattaaatt taatgaatac agcagaaaaa tcacactttt tacttcaacc
     1621 ttcggtgtat tgggggtatt ggtcgctgta ttcctagtca aatctttaga tacctatatc
     1681 ctgaaatgga tcgtgatttt ggtcttacta tacagtgcat atacgatgtt gggcagtcaa
     1741 ttaaaaaaag ttacagcagc taattaagga gcgattcaaa tgttatttct taaaaaagaa
     1801 gatattatca aaagtttttc aatgagagaa gccatcgatg cggacaaaaa agcactgtca
     1861 ttgtattcag caggaaaggc aagtgtgcct cttcgcacca atatcgatgt gccaaaaagc
     1921 aacggacaaa gcttatatat gcctgcttat gtagaaggtg gcgaaggcgc gctaggtgtc
     1981 aaaatcgttt ccgtgtatcc tgaaaacatc aagaaaaact taccaagtgt accagcaacg
     2041 atgatcgtat tggatccaga aacgggtatg gtatccgctt gtttggatgg tacttatttg
     2101 acacaattaa gaacaggcgc tgttcaagga gcagcaacag aacttcttgc aagagaagat
     2161 gctaaaattg gcgcgctgat tggaaccggc ggacaagcac agtcacaatt agaagcaatg
     2221 ttgaccgttc gtaaattaga agaagttcgg atttttgata ttgattttga gcgtgccagc
     2281 caatttgctg aaaaaatgat gcaacaattt tctgtaacga tgcgcccaac aaaaaccagt
     2341 caagaatgcg ttgaaggagc agacatcatt acaagtgtga ccacttcaaa acgtgcgaca
     2401 ttctctgctg agtgggtcaa aaaaggtgcg cacatcaatg gagtcggtgc ttacacaccg
     2461 gaaatgtgtg aaattccacg agaaataatc aaagccgcag atgttgttat ttttgatacc
     2521 atggacggtg ttctaaaaga agctggggac ttcatttcac cgctgcaaga tgggtatatt
     2581 caaagagaca gctatcatgg ggaactaggc caactgatca atgaagaatt gatcggtcgg
     2641 acaagtggag aacaaatcac gatcttcaaa acagttggtt cggctgtatt agatgtggta
     2701 gttgcgacag aaatcgttaa gaaagctaaa gaaaataatt tgggaaaatt gattttctaa
     2761 ttaaaaaggc tgaaattaaa acctttatta ttgcgtttaa aaaaagaaaa caaagaagtt
     2821 tgtaatcaaa tggaaactga ccccataatt taaataaaat aaaaaacatc tcttgttgaa
     2881 ttctagtaat gaagcgaccc taaaacttag accaaaaaat ctaacttttg ggtggagttg
     2941 tcaagaaaaa ccgggcactt agttaaaaaa taagttggac gggttatact aaaattgaca
     3001 ccaaattaag agaaagcgaa gatctggatt atgccaagac gttcatttga tgaaatattt
     3061 aaaattaccg ctgtaaaact ggtaacggaa gaagggtttt ctgttaaaga agttagtcta
     3121 tagctagaag ctcgctctaa tagcgtgtat tgatgagttc aagaagttga aaaatatgga
     3181 taacgtactt ttctcggtag aggaagtcat gaaatccatt cgcagaaatg aaattacacg
     3241 cttagaaaaa gagaataaac ggttaaaaga ataggtagaa ctcctaaaaa ttaccgaagt
     3301 cttcctgaaa gaaaaggctc ttcgtcaaat tgtgttggcg aagacaaatt tgaataaaac
     3361 ttagtccgat atgcggcgcg ttctgtgcgc cgcatatttt ttagacagta aagaaagtat
     3421 aaaaagttaa gggctaaaaa ggctgcacta ccagtctttt agcccttttc attttgtttt
     3481 tgaacaagta aaatattact atgaaagcga atattctcga agcaatcttt tttgacgaac
     3541 accataattg ggataacttt gttgagaaat acgggcgaaa gattcggcta gtcgtcaaac
     3601 gggaagtaaa caagtttaga tattgtggag atattcaaaa aggataccga ctgtttgtct
     3661 gcgagggatg tcatcaaaca aagtttgttc ccttgaaatg tcgtggaaag ttctgtccaa
     3721 cgtgtgcaac aggtgaaagc caaaggtggt cagcggttgt ggcgaatgat atgtttaaag
     3781 tgacacatcg gcacgtaatc tttacgatcg atgaggggtt acgggaaatc tttatgatgg
     3841 aaaactatcg agattcctta ctgaaaggac taatggatga agccgcaaaa attattcttg
     3901 cctattttga gaaaagaaag gtgaaggcag gtatagtagc tacaatccac acctttggat
     3961 caaaactaga atttaatccg catgttcata tggttgttac tatgggtggc ttaacaagag
     4021 atggaagttg ggaagaatac gattatttac ctttttctat gttaagaaaa tactggaaaa
     4081 atgctgtatt gaaactaatt agacgaacgt taggtgagtg ggacaagcaa agagttcaat
     4141 caagattaca agctgcgtac aagaacaatg gagaagggtt ttacgtaaat gctcctaaaa
     4201 ggagcaggac aaatctaaag tcactactcc aatacatcag tcgttatatg aagcgtggcc
     4261 cgatcgcatt aagccgaatc ttaatgtatg atggcgacac tgtcatgttc aactacatgg
     4321 acaaacgaac gaatactaaa gagacgatga ccatgagtat agacgaattc atagctgcac
     4381 tgattcgcca tattcaaaat aagaatttca aaacgattcg tcgctatgga atctatagtc
     4441 gtagaatcaa aacattaatg aaaaaggtca tgaaggagta tcagaaacga gtaaaaaagc
     4501 tgtttatcaa tctgaatgag gccttaagac caaaatcctg ggaagaaaga ataaccgaag
     4561 aatttggtga aa
//
LOCUS       DAYYBH010000130         4461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000130 DAYYBH010000000
VERSION     DAYYBH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.137"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACHAUG_002795"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACHAUG_002795"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM8481826.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACHAUG_002796"
     CDS             2298..3221
                     /locus_tag="ACHAUG_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8481827.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACHAUG_002797"
     CDS             3237..3776
                     /locus_tag="ACHAUG_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8481828.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAYYBH010000131         4434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000131 DAYYBH010000000
VERSION     DAYYBH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.138"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHAUG_002798"
     CDS             complement(543..2048)
                     /locus_tag="ACHAUG_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8481829.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHAUG_002799"
     CDS             complement(2045..2731)
                     /locus_tag="ACHAUG_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8481830.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHAUG_002800"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHAUG_002800"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8481831.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAYYBH010000132         4425 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000132 DAYYBH010000000
VERSION     DAYYBH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.139"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACHAUG_002801"
     CDS             690..1658
                     /locus_tag="ACHAUG_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8481832.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACHAUG_002802"
     CDS             1726..2484
                     /locus_tag="ACHAUG_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFM8481833.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACHAUG_002803"
     CDS             2552..3262
                     /locus_tag="ACHAUG_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8481834.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACHAUG_002804"
     CDS             3298..>4425
                     /locus_tag="ACHAUG_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8481835.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAYYBH010000133         4377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000133 DAYYBH010000000
VERSION     DAYYBH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.140"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACHAUG_002805"
     CDS             73..234
                     /locus_tag="ACHAUG_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8481836.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACHAUG_002806"
     CDS             253..1530
                     /locus_tag="ACHAUG_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8481837.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACHAUG_002807"
     CDS             1540..2196
                     /locus_tag="ACHAUG_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8481838.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACHAUG_002808"
     CDS             2196..3572
                     /locus_tag="ACHAUG_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8481839.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACHAUG_002809"
     CDS             3702..4115
                     /locus_tag="ACHAUG_002809"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8481840.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACHAUG_002810"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACHAUG_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAYYBH010000134         4295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000134 DAYYBH010000000
VERSION     DAYYBH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.141"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACHAUG_002811"
     CDS             <1..792
                     /locus_tag="ACHAUG_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8481841.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACHAUG_002812"
     CDS             889..3138
                     /locus_tag="ACHAUG_002812"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8481842.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4295
                     /locus_tag="ACHAUG_002813"
     CDS             3141..>4295
                     /locus_tag="ACHAUG_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8481843.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1394 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctaga
//
LOCUS       DAYYBH010000135         4282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000135 DAYYBH010000000
VERSION     DAYYBH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.142"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACHAUG_002814"
     CDS             complement(37..1257)
                     /locus_tag="ACHAUG_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8481844.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACHAUG_002815"
     CDS             complement(1277..1888)
                     /locus_tag="ACHAUG_002815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8481845.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACHAUG_002816"
     CDS             complement(1931..2863)
                     /locus_tag="ACHAUG_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8481846.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACHAUG_002817"
     CDS             complement(3284..4144)
                     /locus_tag="ACHAUG_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8481847.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAYYBH010000136         4240 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000136 DAYYBH010000000
VERSION     DAYYBH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.143"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACHAUG_002818"
     CDS             <1..566
                     /locus_tag="ACHAUG_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8481848.1"
                     /translation="YKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIR
                     HHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSF
                     KNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFV
                     RKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            559..1986
                     /locus_tag="ACHAUG_002819"
     CDS             559..1986
                     /locus_tag="ACHAUG_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8481849.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1988..3058
                     /locus_tag="ACHAUG_002820"
     CDS             1988..3058
                     /locus_tag="ACHAUG_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFM8481850.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            3045..>4240
                     /locus_tag="ACHAUG_002821"
     CDS             3045..>4240
                     /locus_tag="ACHAUG_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8481851.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLL"
BASE COUNT         1453 a          518 c          673 g         1596 t
ORIGIN      
        1 attataaaca tatagatgta tatcaaaaac atgattatcc tagaattaat aggaataaca
       61 caactgcatt taaaattttt agagctataa ttaatcgttg tctgaggatg attaaacttc
      121 ccccaaaaat tagacaccat aaatttgatt tgtatcatgg ttctcaatgg tgggctttgt
      181 cgaaagaatg cctaacagaa ttaatacaaa tgtatgaaca aaatcaacaa gactacctga
      241 attttaaaat aggtatgttt gccccagatg aaaaattttt tcatacactt ttttttaatt
      301 cctcgttcaa aaataaaaat gttattggag gaccagattt accacttgaa ttgaaaaata
      361 ttgaagaaac tactttacaa acttcgaaat tagcaaatat tcatattatt gatccttcca
      421 tgaacaaatg gtttgatgaa agggatatag atcaaataaa agaaagtgaa aaattatttg
      481 taagaaaagt taaatcggat tactctgata aactattaaa tagaattgat aaagaaattt
      541 tgaaagttgg aaatatagat gaatagaata gtacctgttt tagagaagag tttattaagt
      601 cgagatgcgt ttcgagtaat aaatttaatc atttgtctgc tttattttat gcctagtact
      661 tttaatgtaa ctaatatccc gatgaaaata gcttttgctt ggggtggaat tatagttttg
      721 tatgaggttt ttataaagaa aaattttttt aaaatgcgtt tttcttggct actgattgga
      781 gcaatgatta gttttatctt aactataatc attaacagag aaaatttttt aattccatct
      841 atttataatt ttggatattt gctgattact ttgatagtaa tttttcctgc agacttttca
      901 caaagtgaag aggaaaaaaa gaaaaaaatg gtgtatttta ataatgtttt cattatcata
      961 atttttttag ctgcgctaat atctatctat caatttattt ttttgatttc ttatcatgtc
     1021 ccaacaggaa cgccaggatt acttgcaaga caaggtttta ttgaacatcg gttatttggt
     1081 gtctatacaa gtccaaatgt tggagctatg tttggatata cttctattat tttaagttta
     1141 attagtgtat taatttcaaa aataacaaaa acgattcgtg ttttttatat gatgaatttt
     1201 atcgtacaaa tattttatat tacactttct tcgtctagag gtgcccaggt agtaatttgt
     1261 tcttttttag tattatttct tttgttgttt gccaattcaa aatttagaaa gagcattaga
     1321 gaagtactac ctgtaaaaat caaatggatg gtaagcatat tattagttct attggtatat
     1381 ttttcttttg gtactaatta tgctaaggat tttttagcaa caattcctgt atcgattgat
     1441 agaaaagtag aagttataga gtcaaatgaa tcagataaaa ctccacttcc agaaaaagaa
     1501 gtaatattac agcattctag tgataactcc gaaatttcag ctggtcgttt tacgatttgg
     1561 aaagctgcgc ttaaagttat aagaccaact ttgttatttg gttatggaga gaccgatttc
     1621 tatgggaata gcgatgcaaa atttttgaaa aaagtagatc ttgatactac agatgtgaat
     1681 gaactaaaaa gagcacatgg aaacatgcat aatggatttc ttcaagtact tgtatcaagt
     1741 ggtataattg cttttatatg tatctatgct ttctatgtgt taaatatctt tagtttactc
     1801 aaaacatttt ttagaagtaa agtgaattat gcgtttcttg gtattgtttt aatttttata
     1861 ctatctattt ttatagatga aatggtggag gctcatgtac ttttcaataa gcgcgatgtc
     1921 atttcaattg ttttctggta ttatttggga agcatttctt acttgtattt aaaaaaggag
     1981 aattaatatg aagacagctt tcattgcttg gactccttta catttgatta atatattaaa
     2041 tgtttatcaa aatttttacc gagaatcaaa aggagattta tttatttatt ctgaatttga
     2101 aaacgctaga agatatagtg agcgtataaa agaattaggg ctatttaatg atgttgtttt
     2161 tgtgaatcac cggaaaatcg gttcttcctt taaacgtaaa attaacttgt taactaacca
     2221 taatcaattt ttctcggatg atttatccgt ttatgatgaa atttttatac agggggaaaa
     2281 ttatttcaca aaaatacttt tctccaatgt ttacaaaaaa aatccagatg taaaactaaa
     2341 ttatatagaa gatggtttgg gtacttattt gaagaagagt atattttcaa caagtaaaaa
     2401 cgttcaaaga gtttttaaaa tattaaataa aaatagcatc ttcaaaaaaa aaattgacga
     2461 ttattttgtc tattgtccgg aattgttact ttatgatgta aaaaatataa aaaaaattcc
     2521 caaaatagat gaaaacagta aattgatgaa aaacatacaa tcaatctttg ggatagaaga
     2581 agtttttgtc ggagataaat gtatcttttt tgatcaacct ttattatctg atggttatgg
     2641 catagatgaa acagatattt tcgctaaagt aaatagtata ttttctggaa aaaaagaaac
     2701 tttaattaag cttcatccta gagcaaggac aaatcgttat ggtgaaaaaa acatattgaa
     2761 ttccagttta ccgtttgaag tcttgctact tgaaaatgac ttctcttcaa cacttttgat
     2821 tagtcctata tctacaattt ctttttcacc atctatgatg tttgaaaaga cgatcaactc
     2881 tattatgttg gctaaattaa ttttaaatga atatacagca ttatctgaag ataaaaaaga
     2941 aacattgaca aatattgttc ttttctgtga acattataac cgtctatcag aagaaaaaat
     3001 tttactccca gaaaactggg cagaattaag aaagttggta gagtatggga ttatttaaca
     3061 cactttccaa gtcaaaatta gttaagtcca gtttctggta tactataggg aattttttta
     3121 ttaagggaat ttcattcata actattccta tttttgtaaa tattactact caagcagaat
     3181 atggtttgat taataatttc acttcatttg tatcaatttt ttcgattctt gttgggctaa
     3241 gcctaaattc ggcaattaat aatgcaagtt ttgactataa agatgacata aatgaattta
     3301 tgtcatcggt actttctcta gctacacttt cgttaattgt gttcttatta atagggaaca
     3361 tatatttttt gttttcaaat gctttttttg atatgaacca acttgtattt aatttgatgt
     3421 tattacaaag ttttgctagt tttgtaatta gttatatcca gtcttatttt acgattaact
     3481 ttaaccattt taaatatttg attttatctt ttatatctac ttttttgaat attgcaatct
     3541 ctgtagcttt gatgcagaca ttatattttg atgataaata tgttggcaga gtagtcggcg
     3601 gaacactgtc atttgtgttg ataggtttag taatttattt aagaataatg ataaagggtg
     3661 ctcatttttt tgataaaaaa cagtggagat acgctttaaa aatctctttg ccgctaatcc
     3721 cacattcctt agcaaatata gttttagcac aattcgatag aattatgatt aattcttatt
     3781 taggtcctgc tgcgggggga atatattcct acattagtaa cataggtatt attctaagcg
     3841 taatatggat gtctactaat aacgcctggg ttccatggtt ctatggtgag atgagtaaaa
     3901 ataatgaaaa aaaagtgaag aaagttagta actattattt agtagtcttt actttgatta
     3961 cgatgattgt aatggtagtt tcgattgatt tagcaaaatt tatggctccg aaagactatt
     4021 attctgggtt atctttagtg gttccgataa ccttaggcta tttctttcag tttttatata
     4081 gtttaccagt taatgctgaa ttttatgaga aaaaaacatc gtttattgcg attggtacag
     4141 tagcaagtgc tatagttaat atactattaa acatgatttt catacctagg ttaggaataa
     4201 ttgctgctgg atacacaacc gtatttacgt attttttact
//
LOCUS       DAYYBH010000137         4039 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000137 DAYYBH010000000
VERSION     DAYYBH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.144"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..774
                     /locus_tag="ACHAUG_002822"
     CDS             178..774
                     /locus_tag="ACHAUG_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8481852.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="ACHAUG_002823"
     CDS             898..2697
                     /locus_tag="ACHAUG_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8481853.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="ACHAUG_002824"
     CDS             complement(2975..3187)
                     /locus_tag="ACHAUG_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481854.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="ACHAUG_002825"
     CDS             3649..3798
                     /locus_tag="ACHAUG_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481855.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1188 a          689 c          711 g         1451 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg agatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatat
//
LOCUS       DAYYBH010000138         3923 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000138 DAYYBH010000000
VERSION     DAYYBH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.145"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACHAUG_002826"
     CDS             complement(573..1130)
                     /locus_tag="ACHAUG_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481856.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACHAUG_002827"
     CDS             complement(1309..1920)
                     /locus_tag="ACHAUG_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481857.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACHAUG_002828"
     CDS             complement(2033..2290)
                     /locus_tag="ACHAUG_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481858.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACHAUG_002829"
     CDS             complement(2887..3165)
                     /locus_tag="ACHAUG_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481859.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACHAUG_002830"
     CDS             complement(3199..3450)
                     /locus_tag="ACHAUG_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481860.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DAYYBH010000139         3863 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000139 DAYYBH010000000
VERSION     DAYYBH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.146"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACHAUG_002831"
     CDS             complement(245..466)
                     /locus_tag="ACHAUG_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481861.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACHAUG_002832"
     CDS             complement(564..809)
                     /locus_tag="ACHAUG_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481862.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACHAUG_002833"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACHAUG_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACHAUG_002834"
     CDS             complement(2033..3034)
                     /locus_tag="ACHAUG_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8481863.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACHAUG_002835"
     CDS             complement(3193..3459)
                     /locus_tag="ACHAUG_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8481864.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACHAUG_002836"
     CDS             complement(3449..3805)
                     /locus_tag="ACHAUG_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8481865.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAYYBH010000140         3851 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000140 DAYYBH010000000
VERSION     DAYYBH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.147"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..1157)
                     /locus_tag="ACHAUG_002837"
     CDS             complement(3..1157)
                     /locus_tag="ACHAUG_002837"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8481866.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1342..3783
                     /locus_tag="ACHAUG_002838"
     CDS             1342..3783
                     /locus_tag="ACHAUG_002838"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8481867.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1232 a          721 c          840 g         1058 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagctgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     1201 gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt
     1261 aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
     1321 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
     1381 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
     1441 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt
     1501 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
     1561 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
     1621 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
     1681 ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa
     1741 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
     1801 catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag
     1861 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
     1921 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
     1981 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
     2041 ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt
     2101 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
     2161 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
     2221 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     2281 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     2341 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     2401 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     2461 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     2521 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     2581 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     2641 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc
     2701 gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca
     2761 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
     2821 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
     2881 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
     2941 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
     3001 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
     3061 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
     3121 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
     3181 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
     3241 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
     3301 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
     3361 agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
     3421 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
     3481 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
     3541 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
     3601 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
     3661 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
     3721 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
     3781 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcag
     3841 atcttgcttt t
//
LOCUS       DAYYBH010000141         3665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000141 DAYYBH010000000
VERSION     DAYYBH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.148"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..278
                     /locus_tag="ACHAUG_002839"
     CDS             <1..278
                     /locus_tag="ACHAUG_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFM8481868.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            421..642
                     /locus_tag="ACHAUG_002840"
     CDS             421..642
                     /locus_tag="ACHAUG_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481869.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            658..1500
                     /locus_tag="ACHAUG_002841"
     CDS             658..1500
                     /locus_tag="ACHAUG_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481870.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1563..3365
                     /locus_tag="ACHAUG_002842"
     CDS             1563..3365
                     /locus_tag="ACHAUG_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFM8481871.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3424..>3665)
                     /locus_tag="ACHAUG_002843"
     CDS             complement(3424..>3665)
                     /locus_tag="ACHAUG_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8481872.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1308 a          519 c          746 g         1092 t
ORIGIN      
        1 aagaactcat caagctggct aataagggtc aagattatga acatctagca gatgagattg
       61 atgagctgag agacaagcga cagctccttt tagtagaaga tgcctccctc agtggcgaga
      121 acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgt accttagagt
      181 acgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa gaacacttcg
      241 tcatatcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta aatagcccat
      301 gattctacag tagagtcgtg ggttttcttt tgcttgctga ttataataat attttaataa
      361 ctattgttat tataaaccaa atgatttata atattcttag cgtaaacttg gaggtgctgt
      421 atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag tgtttccgaa
      481 cttgctagac gtattggaca gactccacag aatttcaata agaaattaca acgagaaacg
      541 gtaaccttag atgagttgaa agcgattgct gatgtgctag gtgtcaagtt tgtgcaggct
      601 tttatattgc ctgatggcaa tgaaataaag ataggtaatt aggaggtgta atttgtattg
      661 aaaatcataa caataaatga tgttgagtat gctgtatttg ctgctaatga aggtacttcc
      721 aaaccacaac ctcatatcat agaaacgaaa tctggtacta ttccagaagg aaaacaactg
      781 agtttattaa aagaatatct taagcagaat gatatttccc caataaaagg agcaacaaca
      841 tattggtgca ttgacaaggt tcttaagctt gattcaagta aagaaaaaac tataagtgaa
      901 acaatccata aacaaaaata cttatcttta actgaagaaa atattgagaa acagcataag
      961 tttgtaggtg caagctctaa ttatgggaaa gaaggtctaa taatacatga tgttttgaat
     1021 gcatttcctc ttcataatga tttaaatact attgcgatga agattgcggt aattgatgta
     1081 acaaattcaa cacatctttc tcaatataag agcaggttgt ctctatatga tttggctaaa
     1141 gtaattctgg agattccaaa ttttgatgat cggttggcaa aaggagatcc tcagttgata
     1201 aatattatcg caaggaacat tggtgcagtt aatatgttct catttgcatc caagtactgt
     1261 acatatcata atgtggaagt gtgtgggaga gatgattact ccatctttga tggtatcgtt
     1321 aaaaatactc ttccacacta tatccaagga ctaactacga ataagattga tacgtggcga
     1381 agaagttttg attacgaggc atttaacgaa tgtgttggaa aactacttga tgaaaataat
     1441 atacatattc cttttagaag aagaaagtta gatcacttct tgtggtatgc gaataggtag
     1501 aatataagaa aaaggcatcg taaaagtgat tctggataaa attaatgggg gaggaaataa
     1561 tagtgggatt atttgatttt ctaaagaaaa aacaaggaaa ggttagtaaa cacgttgatt
     1621 atcaactggc ttctagtcta tttcgtactg gtaaagtttt tgctgaaaaa ttggaagtgg
     1681 acatatcaaa tgtagatgag tttcgtaaaa cttttatcgc ttttgatgtg gaaacaactg
     1741 gcctaaattc ttcttcagat agaattgtag agattggtgc ggtgctattt aaagaaggag
     1801 ttccaattca atcattcgga actcttgtca atccaaaagt tagaattgca gcctcagctt
     1861 cggcaataaa taatataact aatgaaatgc taaactctgc tccaaatgag caggaagttt
     1921 acccccagtt ggtagaattt ttaggagatg cactagatgg aaaaatcact atgtgtgcac
     1981 acaatgcaag tttcgatttt ggatttttaa gtaacacatt ttctagactt gggtatgatg
     2041 ctaaaattaa gtatgttgat actctgagtc tctctagaaa acatataaaa ggcattgtaa
     2101 atcataagca agagactgta gcggaatatt tcggattaat aaatgacact gcccatagag
     2161 cggaatcaga tgcagatata tgcggaaaga ttttttgggg tgttttagat attattgagg
     2221 agtcgttgga agaacaaaaa aagcaaatag aaaagatgac tccagacata aaagaacttg
     2281 aagcttgtgc ttttattcaa gatattattg agaaaaaagg atgcgacacc agttggatca
     2341 ggtatcgaaa aaacagtaat aattatgtcg atgtaacttg tctatataca ttcttaaagt
     2401 ttaagtttgc taagaaggga aattacatca ttattagaaa agatgctaca aatggaattg
     2461 atttacctat agaaccttgt actgtaagtg aaggcggtac aacaaatgtg cgagtctatt
     2521 ttcgtagccc ctttgactta acgcctcttt caaattatat tttcagtgct tatagcgatt
     2581 gttataattc tatgaatgaa tatatgagtg ttagtaatta cactaaaaaa gaagcagagc
     2641 aaagccttag tatgctgaaa tctatttcaa aatcagcgat gaaagagtta ttattcgatg
     2701 caaaaaatcg tggatatgat gagactgaag ttaacctaaa tattgaaaaa gtgatatcta
     2761 gagaagatgt aattattaac gggttgcata atcgagtttc attaaacaag atacgaaata
     2821 tagggaattg ggagaaagga tttgatgcag gttatccata ttgggaacgt ggagaaactg
     2881 ctaggaaaaa tggcaaagta gatgaagcta ttgcactttt tgataaagct cgatataacg
     2941 gctatgatgc tcctgcgcta tatgattctt atgctaaagc ttatcgaaaa attaaggatt
     3001 acgataatga aattctcatt cttgatgaag gaattatgcg taaaacacgg cacgatgttg
     3061 gtactttaga ggctcgtagg gataaggcaa ttaagctatt atttgctaaa caagaggcag
     3121 agcggatagc taaggaaaaa tctgattttc taaaagcgaa caaaaaagag gatgcgatta
     3181 gtgagcctag caagaagcgt ggaagacaga ttattcaaat gactgatgat ggaacaataa
     3241 taaaagaatt cgatactata gcttctgctg taaaagaagt tggcgttaac tcaaaaagta
     3301 ttagggatgc tgcaaatgga gttcaaaaac atgctggagg atactactgg aaatacaaag
     3361 attagcagat aatctaagat aacctaggat cttcttaata gatacccgtg gtgctagtta
     3421 attttaatat cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa
     3481 tagaacactg taagccagta atatgctttc aaatctactc tctagccctt taacagaacg
     3541 tgagttgaaa ttttgacaac ctaatttttc taaggaagaa aaaacagtcc caactgtttt
     3601 tctttgtttt ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg
     3661 cggaa
//
LOCUS       DAYYBH010000142         3602 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000142 DAYYBH010000000
VERSION     DAYYBH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.149"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACHAUG_002844"
     CDS             <1..186
                     /locus_tag="ACHAUG_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8481873.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACHAUG_002845"
     CDS             329..553
                     /locus_tag="ACHAUG_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481874.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACHAUG_002846"
     CDS             568..1437
                     /locus_tag="ACHAUG_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8481875.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACHAUG_002847"
     CDS             1418..2152
                     /locus_tag="ACHAUG_002847"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8481876.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACHAUG_002848"
     CDS             2185..3093
                     /locus_tag="ACHAUG_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8481877.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACHAUG_002849"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACHAUG_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAYYBH010000143         3600 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000143 DAYYBH010000000
VERSION     DAYYBH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.150"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(217..894)
                     /gene="nth"
                     /locus_tag="ACHAUG_002850"
     CDS             complement(217..894)
                     /gene="nth"
                     /locus_tag="ACHAUG_002850"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8481878.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(901..1599)
                     /locus_tag="ACHAUG_002851"
     CDS             complement(901..1599)
                     /locus_tag="ACHAUG_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8481879.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1867..3051
                     /locus_tag="ACHAUG_002852"
     CDS             1867..3051
                     /locus_tag="ACHAUG_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8481880.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3057..3500)
                     /locus_tag="ACHAUG_002853"
     CDS             complement(3057..3500)
                     /locus_tag="ACHAUG_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8481881.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          930 a          720 c          604 g         1346 t
ORIGIN      
        1 ttttataggt atattggaag gcattatttt ctacatcggg tattttccca ataaaaataa
       61 caacttttat ttttatcgca ataaattgta ataagttacc atcctagcta ctgagtatgt
      121 acatttactt tttcatgcaa aaaagataaa cgaaacagct tttctccata ataacaagat
      181 aattcataca tttatttgca attaaagaaa ccacgtctac aggcgtggtt tcttttttag
      241 cgccttttct tttagacgca tccgattttt cccatcttga catattgata gcaaaggaca
      301 tacctcgcac ttgggatttc ttgccgtgca atgatatcta ccaaaaaaaa tcagtgtatg
      361 atgtgtcttt acccataatt cttgtggtac tttccgcatc agtgtttctt ctacttccat
      421 gaccgtagca tctagtttgc agatcctcag gcgttttgag acacgttcca cgtgggtatc
      481 tactgcgatt gctggtatcc cgaatgcatc ccccaatacg acatttgctg tctttctgcc
      541 tacaccagga agagacatca attcttctct tgacgttggc acttgcccat cgaaacgttc
      601 aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta tttcgataca aaccaatcgt
      661 ttttatttta agaatgattt catcgattga agcttctgct aacgcatcgg gtgtcggaaa
      721 agaggcaaac aaatctggcg ttgctttatt cactgataca tctgtcgcct gggcacttaa
      781 gatcactgca atcaataact caaatggatt attgtgcttg agttctccat gtgcttcggg
      841 aaacattcca tacatcgttt ctagagcttc cattgttttt tgtttgctta acatattccg
      901 ctactcctcc aaccaatttt tcagtgagac tttcggcaat tcttcttgtt gagctgactg
      961 ttctacttct ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt ccacttgttg
     1021 ttttgtacgt agattttttc tttcccacga tagcaaaatg cggtcaacgt aattaaaact
     1081 ataagcctga tttaatacgg cttcccgcag tgctagcttc aaaatttccg gctgataatg
     1141 gtcttcttct agccattgtc cgatccgctg gaactcgatc gaagaaagcg ggcgtccaaa
     1201 ttcttgttca aataattgat acactgactg gatttctttt tcttgtgttt tttcttcgta
     1261 ttttttttct tgtgttttta aatactcacc taatagatca tagatggggt aaagattata
     1321 tcgatctgct tttttaccgt tgacaatcgt cgtttctatc ttgataaatc catttgttac
     1381 gagacgatcc agtatttgaa aaatctgatc ttgtttgatt cccatatcca tagcaatcat
     1441 ctgcaaatca gggaaagtat ctccttcaag ctgtgccata tgcaactgta gaacaaataa
     1501 aaattcttca gattgtagac cgatcctatg ataattttta agtaaaagat tagatatgac
     1561 tgtttgtcca gcgcttaagt aattttctaa attcaacatg atttctcctc cgctcaatca
     1621 cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt tacttcactg
     1681 atcggtcaga tttacaatgt cgtctacttg atttttgttt gttcatgttt ttgatgcgta
     1741 tcttcacttt tcggaataaa taaattaagt agatttcgct gttccttaga atgttgacaa
     1801 ggaaacaaaa ttactataca ctatttttgt ttccaagtta acattattgc ataaaggagt
     1861 ttttacatgt acagtttcac aaataaacat tctaaagttt ttggatacgt ctctgtctta
     1921 ttcactggat taggatttac tatcgtcagt cctgtacttc cgtttttaac gctgccttat
     1981 tctcactcca tacatcagca agctttctat atcactttgc tcatgtctgt ttatgctcta
     2041 gctgccttct tatctgcccc cattctcggt agcttaagtg atcattttgg gcgtcatccg
     2101 atccttatta tcagtttatt aggatcaagt atcggatatt tgctttttgg tttaggaaat
     2161 tcgatatgga tgttgttcct tggtcgtatc attgaaggat tgacggcagg agaaatctcc
     2221 accttatatg cttactttgc cgacatcacg gaacctaatg aaagaaccaa agtttttgga
     2281 tggatgggtg ctttagtcgg tataggaaca acgctgggtc ctattatagg gggtctatta
     2341 gctgagttgg ggaattctgt tcctatattt attggagcac tctttacttt cttaaatgcc
     2401 gtttatggct acacatttat gctagaaagc cttccaataa aaaaacgttc agtagattta
     2461 agtttctccc atgtaagacc ttttcaccaa ttacaacaat tattcaagat ttcatccgtt
     2521 attcctttat tgacagcagg ttttgctgtt tggttggcag ctggctctct tcaatctatc
     2581 ttttctcaat tttcgattga cgcctttcag tggaaggctg ggttggttgg tttaagcttc
     2641 tctttgattg gaatcttgga tactgtctcg caattgttca ttatgcctcg attgctcaaa
     2701 aaattttcag aacagcagat cacaagaata gggatgttca gtgaaatcct cgcctatcta
     2761 ttcatcacat tatctggaat tcttctactg ccgattttgt ttttatttgg gattatctgt
     2821 tatggtttcg gtgattctat ttttactcct gtattcaatg gccaactatc caattctgtc
     2881 tcagataacc agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc tctatcgcgc
     2941 gtcatcggcc cgctaatcgc aggacagctt tatgctgcag caccatttct tccgacggta
     3001 ttcggatttt tgcttatgat ttttgcctat tatcactaca ataaaaaata gtagcttcat
     3061 ttgctacttc gtataaattc tctgtatcga ccgataaaac gaaatactgt gtcaaattct
     3121 tcttttgaca tattttcaaa tacagtagca tctcgctgta cccatttttg atggagttgc
     3181 tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat ttctttttta
     3241 ttctcttcgc tttgataagt gtttatgatc cctctagcta tcagtttttt tgttagctta
     3301 ctgatggctc ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt aggatcaggg
     3361 attttcccga tatgttcaat cacttctagt tcatttagag taaatcctgc aaattctttt
     3421 tccatttctg gtttattacg atgaataatc tcattagcta aatccagata ttcttctatt
     3481 agcagttgtc ttttatccat ttctacacct tctttttctt tcattatatt gtttttttgt
     3541 ttcctattca acaaagataa aaatattttg taggaaaacc caatctgaaa ccaagaaaaa
//
LOCUS       DAYYBH010000144         3419 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000144 DAYYBH010000000
VERSION     DAYYBH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.151"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACHAUG_002854"
     CDS             complement(<1..2177)
                     /locus_tag="ACHAUG_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8481882.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACHAUG_002855"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACHAUG_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          963 a          754 c          539 g         1163 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctat
//
LOCUS       DAYYBH010000145         3419 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000145 DAYYBH010000000
VERSION     DAYYBH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.152"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1190
                     /locus_tag="ACHAUG_002856"
     CDS             150..1190
                     /locus_tag="ACHAUG_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8481883.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2014..2346
                     /gene="traE"
                     /locus_tag="ACHAUG_002857"
     CDS             2014..2346
                     /gene="traE"
                     /locus_tag="ACHAUG_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8481884.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2965..3306
                     /locus_tag="ACHAUG_002858"
     CDS             2965..3306
                     /locus_tag="ACHAUG_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8481885.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1269 a          461 c          648 g         1041 t
ORIGIN      
        1 agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa cagttttaat
       61 gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat tttacataaa
      121 taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt aaggcggata
      181 gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct gacgaataca
      241 aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga ttagagatcg
      301 caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat acgacaaatg
      361 aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa aaagagttgg
      421 aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta gggaaaaata
      481 atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc tacttactcg
      541 aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg aaaatcatac
      601 ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt atgaaaatca
      661 taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa cttaataata
      721 atatattaga cactaataga cacaatatag acactgaaaa agaccgccta caagatcaat
      781 tgttgttaga caattttgag acaattatga caaacgacag cattgctacg tttgtccctg
      841 aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct caaaaaaccg
      901 tccagactat tcataatgca aagaaaaaag ctgaaataga aagtgatatt tcgatagttt
      961 ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg acactgttaa
     1021 aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg atttttgtct
     1081 atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt gaaaaacgtt
     1141 tgaattatgc aagctcccca agcactatta cgaaagactg gttagagtga aactttactg
     1201 acaaagtgat tgttgcttca tggagtaaca ttaaaattaa aaatttttta ttattctaat
     1261 tatattacga atattacaaa ataatagcta ttttaataga atttataagt taaaatatta
     1321 tgataaaaat cacaaacgta gccttttacg tcataagtgt taaaagaaat aattaatttc
     1381 aaatatagaa aagggaacgt atgtttgcat actccttttt tttgagttat aataatacgc
     1441 gaagagagat aatttcatta tttaaacaag ctaaaaatct tgatattact tcttttcagg
     1501 caatacgaat attattcgct tacatttttt gtataaattt aattgtctgc tatgcttgtg
     1561 atgaattgat tttcgttttg cacttttgga tgataaattt ggacaattga aaatacaaaa
     1621 aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc gtcagcgttc atacacggca
     1681 ttaagatttg gcccttaatg cgttaacctt tcagtgttac ggtattaccc gcaatggatt
     1741 tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag gttaaaaata
     1801 cgtgatatta cgatgaatct atgcctaagc ataatgttat aaaaatgtcc gtgtggctct
     1861 ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttggatgcgt tccctctttt
     1921 ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga aaaacttgtt
     1981 aaaaatattt aaagaaattg ggtggtttat tttatgtcat atacaggaat tttgagtctc
     2041 aaagatattt gtcactacgg taaacggtgt accgctacag agaaaatcac gaagaaacta
     2101 tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat atataatcca gaaagacaaa
     2161 gtctcagagg aaatgattta ttacattggg aaacaaaagc aaataatttt aaaagatcct
     2221 atccccttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa cggtgtactg
     2281 attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg gcgtttgatc
     2341 ggctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct tgaaaatatt
     2401 gtcgagactt ctaaaggaac ttagagtcac tagaaaaata aggagtagac ttgatctatc
     2461 aacaattccc ttcggggagt gacggggtag gttttaacga ttggtaacgt aaaaaattgg
     2521 tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg aatttgtgta aataataagc
     2581 gcagagtaaa caagagggaa tttagaagtt agacaagtta gaatagcgtc tagattaatc
     2641 atgagcagct actaagggat ctagactttg acgcaaactc atggaataag gagtaggtct
     2701 tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca tgtagatact
     2761 tgcgaggacg ttgattattg gatcaatcga gcatttaccg ctgtctacta gggtttctcg
     2821 tttcggacgc cataaacgag tcgaaatttt ttatgtattt ttaagtaaac gaaaaacgat
     2881 aaaatatgaa caagtcctga ataaagtgaa aaaattaata taaagcatag agaaaatgaa
     2941 atttacgtaa tggaggaatg acaaatgaaa aattatgatc cgaatatacg attgggcaca
     3001 catactatta aggtttcttt ccaacgttgg gattacaaag gatttgtgac gtttagaagg
     3061 ggcggaaatt gtaaaggatt agatgtttta gcattagacg aggatgattt atacgatcaa
     3121 acattgactg ataatccaat tggattcggc ttgctacctg aagatgatga aggtaatgaa
     3181 tggtttaaaa tgacgcttat gaatgataat ggagacgaat tatctgttga agatacttgg
     3241 tcatatttga gtgattatat tgtcagcttt gaaatcattg aatttgtagc ggataaagaa
     3301 gaataaaaac tggaggaata aattttacag ataaaggctt agaaggagtc acgacgatta
     3361 ataaaaaggt agtattacct taggcattga gtaaggaatt tatttagtct aatggtgat
//
LOCUS       DAYYBH010000146         3232 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000146 DAYYBH010000000
VERSION     DAYYBH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.153"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..668
                     /locus_tag="ACHAUG_002859"
     CDS             <1..668
                     /locus_tag="ACHAUG_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8481886.1"
                     /translation="AYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNG
                     VSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIK
                     GALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHL
                     PITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTL
                     YAT"
     gene            658..1398
                     /locus_tag="ACHAUG_002860"
     CDS             658..1398
                     /locus_tag="ACHAUG_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8481887.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1395..>3232
                     /locus_tag="ACHAUG_002861"
     CDS             1395..>3232
                     /locus_tag="ACHAUG_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8481888.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1210 a          395 c          628 g          999 t
ORIGIN      
        1 cggcatacaa tacgtggatt tctataaaaa ataccatgat taatacatgg aataacatta
       61 aggactcttt ctggaatatt gttactggaa ttgttaattc tgctgaaaat gcatggacaa
      121 atctaaaaaa tggtgtttca aatgctatta atcgggtaaa agaaatcttt aattcgttaa
      181 gggaagtcaa tttatttgaa attggcaaga acatcattga tggacttata aatggtgtaa
      241 aagaaaagtg gaatgctttg aaagatacta tcaaaggtat tgcaggtaat atcaaaaatt
      301 ggattaaagg agctttagat attcattctc catcaagatg gatgcgtgat atggttggta
      361 aaaatattgt tcaaggtatt atcgttggta ttgataaaga gcaaagtaag ttagatcaaa
      421 ccatgacaaa tttagtaaaa acaccgtctg ttcaacctgt agtaaatgga ataagtcacc
      481 taccaattac acaaacaaag caaaatatat ctgctagtga aacaaaagaa attcatttac
      541 atttaaatgt ttatggagat ttacctgatt caatggttcg gaagattacc acaaaaatta
      601 aaacagaatt gacaagacaa atgaaacgag atgctggtgc agtaggagga acattatatg
      661 caacttaaga gaggacaatt ttttattaat caacattatt cttctgaatt taatgtgtat
      721 attcaaaaca ggcctgcttc tgtttcagct agtcgagtaa ttgaattgag agagcgagaa
      781 ggaaatgatt ctatcattat tgataaaggc tattacaaaa atgtgactag aaagattgag
      841 tgttactata aagcaccttc aattgatatg gtgcaggagt gggaagatcg aatcactgaa
      901 tggttagata tgtcgtctta tagtgatttt attctttact atgatgaaca atatatttat
      961 caagctgtag taatagaagg acctgaattt aaaggaacaa gaaaaacagg aaatattgtc
     1021 ccttttgaat ttacagtgag tattcgacca tttaaggaaa attacaatgg tagatttact
     1081 attcgacaaa cagaaacttt tgaaatttat aacccagaga agtattcttc aaaaccgctt
     1141 attaaattga gtggttctgg agatgcttct ttttatatta ataaggataa atatgatttg
     1201 aaatcgttgg acagagaatt atttatagat tctaaattag aagaggctta tcgaaaatta
     1261 gatggtaatt tagaacatca ggatcaggtc accttatttt tagattttcc atttttatat
     1321 cctggaaaaa atgaaattaa atggacgaaa aatattcatt catttgaaat aatgcctagg
     1381 tggtggagaa aagtatgaaa ccaagaatat atagtcctac tgaaacagat ttctcaacga
     1441 atggcttagg aattttaaag gattgtacaa gatgtggaat atatgaagta gctaatggaa
     1501 aatatgaatt agagttggat tatcctttag gaactagatt tgatgaatat tttgaaaatg
     1561 actatcaaat aaaagcaaag ccaaacgatc aagaagagta tcatatcttt tttattgatg
     1621 ataaagatat agatactttt ttaaatacag taactattta tgctcagagt cgtacaaatc
     1681 gacttggaag acgggtagtc actcttgtgg aagtggactc taaaactggt caagaagcga
     1741 tatcaattat tgaaactaaa atggataaaa aatctgacat acgactttat tctgatatta
     1801 cggctgtttc tagcacaatc tttgaagcga gaaatgtttt aaattgtatt gctggtgaac
     1861 aaggatcatt gcttcaatat tggggcggag aaattaaacg tgaaccattt aagctttctt
     1921 tgttaaagcg aagaggtcgc aataatgttg gaacgattcg gtatggaaaa gatatgtctg
     1981 gtttgaaggt caaattagat tggacaggta taaaaacaag aattattcca tatgctgatc
     2041 ctcaaagtga agtaggtacg actaatcgaa tttatggttc gccagtagat agtgcctata
     2101 ttaataacta tcctgatgtc tatacagaac atgttcagtt tacagaagaa caaggagtaa
     2161 aagacattaa gagcttaaat aaaatagcta aaaattactt taaaacgatc aatcctggct
     2221 gtgataagcc taaagtttct attacggttg aatttgataa gttgactgat agtgaagaag
     2281 cgaaagaatt tgcgaagatt agaaattatg gtctgtttga tacatttaaa atatatcata
     2341 aaaaatataa tatttatctt gaatcaaagg ttagtggact acaatacgat tctttatctg
     2401 aaaaaacttt gaaattagaa gcaggagaca ttcaagttgc tttttatcag caacaagctg
     2461 taactattca ggataaatta aaagattatg caacgaataa ttatatgagt gattttaatg
     2521 attatgtttc ttcaatgatt acaggtcaag gcagtgcagg aggatatgta gttttatggc
     2581 caaaagaaaa accttccaac atctttatta tggataatcc agatttagaa aaggcaagag
     2641 aagtactaag aatgaataaa aatgggattg ctttttctaa gaaaggatgg cgggggcctt
     2701 ttaattcagc ttggacatta gatagtatat ttaatgccaa ttttatacaa actggattaa
     2761 tcaaagcaga tatctttcaa aattctttta acaagactgg agatgtattg aaattagtta
     2821 atggactact ccaaatttgg aacaacaaaa agaaaataat ggagttaact aaaaaaggaa
     2881 tggaattttg gaattctaag ggttcaattg gaacgattgg aacaactgat tctgttggta
     2941 atccttttcc tggggcttct actccaaccc ctattgaaga taattcttta gttattcgta
     3001 caaatggaga cggcaaatat attttgattt ctcctaaagt aggaaaagga ttagttttat
     3061 taggaaatgg taaagcaatt tattttggag acttagatgt acaaggaaaa cttacagtta
     3121 aaggaaaaga aataattcct ggacaaggtg gtggtccatc gggaggtgga gaatctactg
     3181 gtgggtatcc tagtgaatta aaaacagatg cagaaaagag agcttggaga at
//
LOCUS       DAYYBH010000147         3200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000147 DAYYBH010000000
VERSION     DAYYBH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.154"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHAUG_002862"
     CDS             301..585
                     /locus_tag="ACHAUG_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481889.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHAUG_002863"
     CDS             588..1004
                     /locus_tag="ACHAUG_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481890.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHAUG_002864"
     CDS             complement(1141..1860)
                     /locus_tag="ACHAUG_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8481891.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACHAUG_002865"
     CDS             2522..2608
                     /locus_tag="ACHAUG_002865"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8481892.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..>3200)
                     /locus_tag="ACHAUG_002866"
     CDS             complement(2911..>3200)
                     /locus_tag="ACHAUG_002866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481893.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAYYBH010000148         2929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000148 DAYYBH010000000
VERSION     DAYYBH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.155"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACHAUG_002867"
     CDS             85..981
                     /locus_tag="ACHAUG_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8481894.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACHAUG_002868"
     CDS             1073..1288
                     /locus_tag="ACHAUG_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8481895.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACHAUG_002869"
     CDS             1306..1578
                     /locus_tag="ACHAUG_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8481896.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACHAUG_002870"
     CDS             1580..2443
                     /locus_tag="ACHAUG_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8481897.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          510 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAYYBH010000149         2736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000149 DAYYBH010000000
VERSION     DAYYBH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.156"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..516
                     /locus_tag="ACHAUG_002871"
     CDS             172..516
                     /locus_tag="ACHAUG_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8481898.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ACHAUG_002872"
                     /pseudo
     CDS             517..936
                     /locus_tag="ACHAUG_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="ACHAUG_002873"
     CDS             complement(1028..1378)
                     /locus_tag="ACHAUG_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481899.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ACHAUG_002874"
     CDS             complement(1344..2675)
                     /locus_tag="ACHAUG_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8481900.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1015 a          493 c          527 g          701 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactat
//
LOCUS       DAYYBH010000150         2501 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000150 DAYYBH010000000
VERSION     DAYYBH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.157"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..845
                     /locus_tag="ACHAUG_002875"
     CDS             <1..845
                     /locus_tag="ACHAUG_002875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8481901.1"
                     /translation="HMSYRGSFSRKYVLNKWFRLLNIPVIIHLHGSEFKKWYDSVSVK
                     KQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENSIVVPEYTIKWNSKMCRF
                     LFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDEEISLKELIEEYNLQSYF
                     EFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYGMPVIATDVGDISSAVRD
                     GENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAITKFNDGNFFDRILNLYQK
                     GSKE"
     gene            1413..2474
                     /locus_tag="ACHAUG_002876"
     CDS             1413..2474
                     /locus_tag="ACHAUG_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8481902.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
BASE COUNT          939 a          265 c          484 g          813 t
ORIGIN      
        1 tgcacatgtc gtacagaggg agttttagta ggaaatatgt gctgaataag tggtttagat
       61 tattaaatat tcctgtaatc atccatcttc atggatcgga atttaaaaaa tggtatgata
      121 gtgtttctgt aaaaaaacaa aatcagattc gcttgctgtt aagagagtct gattccacga
      181 ttgtacttgg agaaaaatgg aagaagataa ttaaagaaat tgagccaaat tgtaaaactg
      241 ttgttgtaga aaattctatt gtagttccag aatataccat aaagtggaat tcaaaaatgt
      301 gccgatttct gtttatggga gttttgataa agcgaaaagg tgtacacgat ttggtagaag
      361 cagttaaaaa cattgcagaa caatatccag aaaaaaagtt tatttttgag attgctggag
      421 caggtgatga agaaatatcg ctcaaagaat tgatagaaga atataatttg caatcgtatt
      481 ttgaatttac aggttgggtt gtgggagaaa aaaaggagga gtgctataga agatcaaatg
      541 tgatgatttt accgtcctat aacgagggat tgcctatatc tattctagag gcgatttctt
      601 atggaatgcc tgtaattgct acagatgtag gagatatttc ttcagcagta cgtgatggag
      661 aaaatggata tttgtttcaa cctggtgacg tatcagcgtt gacatctgca atactaaaaa
      721 tcaatgataa agataaatac gtaaaatttt cacgttgttc gaaggatttg gcaataacga
      781 aatttaacga tggaaatttc tttgatagaa ttttaaatct gtatcaaaaa gggagcaagg
      841 agtgatgaca aggaaaataa aaaatgtttc gattatgtaa tgaacttatt aagtgaataa
      901 aatagagaga aaaaatattg aaatagacga tgtaaatttt tatccgtgta agaattcaaa
      961 tgaatagtag tgttttagtg cttgtaatag agcaagagta caagcacggt acattatata
     1021 aaaacattaa tgatatttct ataaataaat agattttgta ttctggatga tgaagaggaa
     1081 gatattatct gataacttac cctaaacttt gcagggggtg gttataagtt gtgtatatgt
     1141 ggcagtaatg aatgagtgaa ataatcatat taagaaataa aattgcaata ttgtgtaata
     1201 caaaagtata ttttcattat tccaagggaa agcatactta cgaggatcat attattgatt
     1261 taataaataa tttaaaggaa ataatttttc tagaagaaga tagtatgtca ttcacatcat
     1321 caagatcaga gtctattttt ttcagagaat ttaagaaaat cgttgatgcc gtcattgact
     1381 aattgagaaa acgtgagtga aaggatagaa ggatgaattc gagtgatcta attagtgtaa
     1441 tcatacctgt atataatgtt gaaaagtatg taggaagttg tttggaatca gtgtgtaatc
     1501 aaacatataa gaacctagat attataattg tggatgatgg atctaaggat tcatctaata
     1561 aaatatgtga agactatgca ttaaaggatt caaggattcg tattattcat aaaaataatg
     1621 ctggcttggg attggcaaga aatacaggac ttgaacatgt aaaaggaaaa tatgttgctt
     1681 ttattgattc tgatgatttt gttgcagcta attatattga agcaatgtta aatggtataa
     1741 aaaggagtaa tgcagacaca tgttatgctg gatattatga atattatgat gataataata
     1801 ttatttcaaa gccagcaaga tatgatagta cgatatttac aaataacgct ataattgaaa
     1861 aagtgttgct tggaatgatt ggcactgagc cgagttataa agtcgatttt atattgccaa
     1921 tgtcagtttg gcatgcgctg tattcatctg agataattca acagcacaat atcaagtttc
     1981 cgtcagaaag ggattatata tctgaagata tcatattcca tatttcatat ttgcaacatt
     2041 caaaaataac aagttttatt aaagattgta tctattatta tagatgtaat aataataatt
     2101 cattgacaca taattttaga gataatgagt tcgattgtaa tattgaatta gttaaaaaaa
     2161 ttgaaggcga tttaagctat atattagaaa atgagatatt tgtaaacaaa acaaggagac
     2221 ttttgttggg acgctgtcgt acttgtattc aaaaggcagt tagatatgct agttttaata
     2281 aagaatttga tttaaactcc tatttgtcta agatacttat gaacgatgag ttgattgcct
     2341 gtattcactc atacccatat aaacaaaatc caattaaaca acgaatattt aactattttt
     2401 tactaaacca atccattatt ggggttaaaa cattagcata tttaagcgga atgggtagaa
     2461 ataggagatt ttaatcataa aaaactgtac tatagtgaat t
//
LOCUS       DAYYBH010000151         2459 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000151 DAYYBH010000000
VERSION     DAYYBH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.158"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..1150
                     /locus_tag="ACHAUG_002877"
                     /pseudo
     CDS             6..1150
                     /locus_tag="ACHAUG_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="ACHAUG_002878"
     CDS             1321..1779
                     /locus_tag="ACHAUG_002878"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8481903.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="ACHAUG_002879"
     CDS             1938..2408
                     /locus_tag="ACHAUG_002879"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8481904.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          460 g          716 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DAYYBH010000152         2405 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000152 DAYYBH010000000
VERSION     DAYYBH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.159"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..103)
                     /locus_tag="ACHAUG_002880"
     CDS             complement(<1..103)
                     /locus_tag="ACHAUG_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8481905.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(135..458)
                     /locus_tag="ACHAUG_002881"
     CDS             complement(135..458)
                     /locus_tag="ACHAUG_002881"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM8481906.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            920..2302
                     /locus_tag="ACHAUG_002882"
                     /pseudo
     CDS             920..2302
                     /locus_tag="ACHAUG_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          718 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc ctttttttgt
     2341 aaaaaactat tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc
     2401 aagaa
//
LOCUS       DAYYBH010000153         2398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000153 DAYYBH010000000
VERSION     DAYYBH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.160"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHAUG_002883"
     CDS             207..2069
                     /locus_tag="ACHAUG_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8481907.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACHAUG_002884"
     CDS             2069..>2398
                     /locus_tag="ACHAUG_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8481908.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAYYBH010000154         2395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000154 DAYYBH010000000
VERSION     DAYYBH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.161"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHAUG_002885"
     CDS             316..570
                     /locus_tag="ACHAUG_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8481909.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHAUG_002886"
     CDS             571..888
                     /locus_tag="ACHAUG_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8481910.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHAUG_002887"
     CDS             1447..2382
                     /locus_tag="ACHAUG_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481911.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAYYBH010000155         2392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000155 DAYYBH010000000
VERSION     DAYYBH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.162"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..2244
                     /locus_tag="ACHAUG_002888"
     CDS             91..2244
                     /locus_tag="ACHAUG_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8481912.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          816 a          424 c          531 g          621 t
ORIGIN      
        1 agaatttctt gctccgccca ttttttcttt tttattaaga agtttttggg tacaatgaag
       61 catgaatgta aataggcgga aggataggaa atgatgaaag attttatgaa aaaaatatta
      121 aatacttctt ggttagaaaa aataagaaaa ggtgcacaag aacctaagaa caaaacgttt
      181 cgccggtccc atgttccttt tcgcttaaat tttctttttt tcattatatt tacgttattt
      241 gttgcgttga ttgctagatt gggttatttg cagatcgtga acggcgaaga gatggaggcg
      301 cgagtgaagt ctacttctac gattacggtc caagaaagta ctcctcgcgg aatgatctac
      361 gactccacag gaaaagcttt agttacgaat aaagcgaatc aggccatcac atatacgaga
      421 ggaacacaag ttacggctaa agatttgctt gaaatcgcaa cgaagctgaa ctcgctgatc
      481 gatgttccgg tagatgaaaa tctgacagat agagataaaa aagatttttg gctggctgat
      541 cccaaacatt tgaaagaagc cacaaaaaga ctttcggcaa aagaaaagca attagggaac
      601 agcgaacaat atagtgcgat ggtcaataaa gtcacagctg atgagattaa tttaaacgag
      661 gagcaattaa aaattgctac catctttaaa cggatgaata gtgcatatga attaaacact
      721 gtctatatca aaaactctgg tgttactgat caagagttag ctgttgtggc agaacgcgct
      781 tctgaacttc caggagtttc gactggcaca gactggacac gagaatataa tgcagcaagt
      841 tcactgaaaa gtattcttgg cagtgtgacg actgaaaagc aaggacttcc agcagatgaa
      901 gcagagaaat acctagcaaa aggttattct cggaatgacc gtgtaggaca aagttatttg
      961 gaaaaacaat atgaagatgt cctgcaagga accaaaaccc aatatgaagt ttcccttgat
     1021 aatgaaggaa atgtttcgaa tcaaaaagaa atcttctctg gggaaaaagg ttctaattta
     1081 atgttatcta tgaatgcaga attccaatca aaagtagaag aaattttgaa aaggaattat
     1141 caaacattga tcaacaacgg caaagcacag tattcgcctg gtgcttatgc agtggctatg
     1201 aatcctcaga caggagaagt gctagcaatg actggtttca gtcatgaaca aggttccaaa
     1261 gaaatcaccg agaatgcttt aggcacgatt actagcgcct ttgatccagg gtctgtagtc
     1321 aaggctggta ctttgacagc aggctgggca agtaacgcga tcagcggaaa tcaagttttg
     1381 attgatgagc cgattcgtct gcaaggtgct tcagaaaaaa gctctgtctt taatcgaagc
     1441 ggacaagttg ctcttgatgc agtcaaagca tcggaattgt cttctaatac gtatatgatc
     1501 aaagtggcgt taaagatgtt aggactagac tatacacctg gaatgggact acctagttta
     1561 gatgaagaag ctaaagctta tcagcaattg agagactctt tcaaagagtt tggtcttggt
     1621 acaacgactg ggatcgattt gccgaatgaa tctcctggga tctcacgttc tgttgattat
     1681 atgaagaagt tcaatgcgga taatggcaaa gaatggtatg ctccaggtaa ctttaccgat
     1741 ttggcatttg gacagtttga cacatataca ccaatccaat tagctcaata tgcttcaaca
     1801 gtcgctaatg gcgggaaacg agtacagccg cacctagtca aagcgatcta tggcaatgac
     1861 gaaaatggaa acttgggtga agtgaagaaa gaaattggca caacagtaga aaatacggtc
     1921 aatatttctg cagaaaatat ggcgatcctc cgtgaaggat tccaccaagt tgtccatggt
     1981 accgatcctt acacaacagc caaaccatta gccagtgcga aaatggacct ttccgcaaaa
     2041 acagggactg cagaaacggt tgcggaaggt catccagata ttgcgacagt caatagtaat
     2101 atcgtggcat acggaccaac agacaatccg gaaattgcca tcagtgttgt cttgccgaac
     2161 ttattagacg aacaagacca cataaacttg acgattgcta aagaaataat ggacgcgtat
     2221 tatgatatgt tcatggcaaa ataaaaaaga ggtcgtgaaa aagccgctta gtttcgagta
     2281 tgaagaaaaa ttttgtgaaa atagcttctc acatttttgc ttaaatttga cttcataccg
     2341 agaaactggc ttttgaacac cgtttactcg gcaaaaaagg ttgtgacaaa aa
//
LOCUS       DAYYBH010000156         2377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000156 DAYYBH010000000
VERSION     DAYYBH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.163"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..735)
                     /locus_tag="ACHAUG_002889"
                     /pseudo
     CDS             complement(334..735)
                     /locus_tag="ACHAUG_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
     gene            complement(924..2243)
                     /locus_tag="ACHAUG_002890"
     CDS             complement(924..2243)
                     /locus_tag="ACHAUG_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8481913.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          739 a          407 c          452 g          779 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgatc
      721 cctgaataat tcatagcttt aattctttgg cacattttat tgaaatatgt atcactaata
      781 tccctatcaa ctgattttga ctaaaaagtt acttcaaaca ttcactgttc aaaaatattg
      841 aggaataaat tttgggtgta aggcattttg agcatacttc tccctggata acttcccagt
      901 aaaaaaatcg ttagattgtt ggattagatc cattgactgg ctcgcctcag gcgagcaaag
      961 accctgtaga attcattctg atacacatga taactagata atttgagata gactcgtcta
     1021 cccgtttgga ctaatttccc agcaactttc agaaacttca atcgaataga atgaatggtc
     1081 attccctttt ggacttcttc aaagccaatc gttcttaaga agttgactaa gttgtaagct
     1141 atcaagctga gcatcattcg gacatgattc tccaaaaagc gaggactgtc tgttttgtca
     1201 aagtaaaagc cagctttcgc ttctttaatg aagttctcca ttgtgccacg tttggcatag
     1261 atagaaaaga tggtatcagg agaaacattt tctgatagat tcgtcacgat aaattcatgt
     1321 cgaaagagta gttcgcccgc ttcacgtgtt gagcgtatac acacgcgacg actttgtgac
     1381 caggattgtg cttgataagt ggtcgagaag tactgaactt ctcgttcttc ccacttttga
     1441 ttatcgccat aaagtactga tttctcagct atttgaccta gtctacgatt attcttcagt
     1501 cgaataacat aatgactctt ttttgattca cacgaatcat acacaccagg tgtagcgaac
     1561 ccgctgtctc cacgaaccaa gatgtcagtg tttggtagag agtgattata gtgctctaat
     1621 aaaggtgtga gaaactcctt tacgcctttt gatgtatatt gatttcctga acgtagttca
     1681 gcttttaaga agtcaccagt caatccgtca aaagctacca atggatggta accataggtt
     1741 tggtagtgtg tattataatt cgtttgttct tggtgaccaa atgtatctga atgggtcgaa
     1801 tctaaatcaa tgattaattc cgtatcatta cggatgaggc gtgctttatc gataagttct
     1861 tggttcaaag cttgaagttc atggatattc tcctcagata gtcgatctaa aaatcgggaa
     1921 agtgaagatt gagaagcgag ttctttccta tctaaaacag ctttaaacac aggatcttgt
     1981 ctcaggagat tagctgcaga atcagctgag taaccagcaa ttaattgcat aatgaactgc
     2041 tcaagtatgg ataagttgtc atgagtaaaa tacgctcgtt cgtcttcaat gtgaatgtgt
     2101 tgcttagcca gttcagaaaa accaaagatg ttcatcagct ctttcactag gacgagaccc
     2161 gaatcactcg ataattgacc acctgtatga gaaatagtga tatttgaatt gaattttact
     2221 tggtttttgt gtaagctagt catgaaaaga actcctttct tttggttggt ttagtcactt
     2281 taaccatagc agaaaggggt tcttttttca tcacttaacg ggtgaagatg aaaaagtaat
     2341 tgtaagctgc cgtgaggcag tttcacatgg ttttaat
//
LOCUS       DAYYBH010000157         2217 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000157 DAYYBH010000000
VERSION     DAYYBH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.164"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..384)
                     /locus_tag="ACHAUG_002891"
     CDS             complement(85..384)
                     /locus_tag="ACHAUG_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8481914.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            735..1367
                     /locus_tag="ACHAUG_002892"
     CDS             735..1367
                     /locus_tag="ACHAUG_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8481915.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1490..2128
                     /locus_tag="ACHAUG_002893"
     CDS             1490..2128
                     /locus_tag="ACHAUG_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8481916.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          733 a          358 c          326 g          800 t
ORIGIN      
        1 acagactctg ttcaaataaa tgaacctagc ctgcattatg agattgacta tactttttaa
       61 ttcattgaga tgaataactc gtttttataa atgcccaaaa gtaatcggtg ccgaagccat
      121 tcctcgacct aattcttttt caaattttcc tattgttgat atctccatat aaaacggtga
      181 tactggttta gacaattctc ccctgattgc taacggacgt aaactacgtt ccaaattatt
      241 tatgacttta ggataatact gacctttttc taagcctgtc tttgcgtcta tcaaaagtat
      301 acgttccttt tctttgatct ctggatttaa aacgagatca tataatttta gtaccatgtt
      361 ttctatttct tcacttttag acatcacgat ttcctcccaa aaatatattt cttcccacta
      421 cattctataa acaaagtata cgctacatct agattttttt aaatcattac tggattgtta
      481 aaacctttca atacactgtt atggcaacga tttttatttt aaaaacattc taaattaaat
      541 cagtaaaaat ttttattcta agatttttat tagaattacg cctaatttgt gtctctcagg
      601 gcaattctaa gaacgtttca agagatagcg acactatcgt tatcgaaaaa ggcgaaaacc
      661 tatctcattt tctgctaatg taagttggtt tagcttttgt taatatgact ttatgaaaat
      721 ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc aagcttgaat aaaggatcct
      781 tttttcatat ctatgaaaac gaagattttg aactgtataa tatcctttca caaaaagagg
      841 tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa atctattttc aagtatgcat
      901 tttttgcttc caatgatttt caaagaatgg agattaccga ctgtatattc gaaaactgtg
      961 atttttcagg ctgtatattg acaaaaagca tgttgcatcg cgtgaaattt gtcaattgta
     1021 aattaacagg tactaaattg atggaaagtt atattgggaa cacgttattc gagaattgta
     1081 aaatggacta tgtaaattta tctggttcaa acataaaaga aagtaccttt gagcactctg
     1141 tcttatcaag cgcagatttt gtagactgtt ccctcactaa aataatgttt tctactaatg
     1201 atttagaaaa tgctagcttt tttgatactg atttaaaaaa tatcgattta agtagcaact
     1261 cctttgaaat gattgaagta cgctcggaac atctaaaaaa ttgtacgctt aatcagttgc
     1321 aagcacttgg ttttgctaga aaatttttac aaataaaagt agtataatca aaatgaatat
     1381 ataagataaa tttttagcaa tacaaatatc cataaaaaaa cattgcctat tttaactctt
     1441 catgtatact gactctatct tcaaaaactg aatagtagga gttattatca tgaataacat
     1501 tttaggaaaa aaaatagaac ttaaaaaagg gatattgggc actattgtcg ttgtactcgg
     1561 tttgttaggg ttgctatttg cgccatccat tattagcatt ttttctttaa ctatcactgg
     1621 atttatagca tttacacttg tttttggtaa aaaagatacg aaaaaaatgt tctcgaaacc
     1681 tgtcgctcct gtcaaaaata tagccaaata tttttttatc aatgtagtaa tcagtgccgc
     1741 agtctcggta tttttgcaac aaattttgaa atggggttta acaggcaatc caataaatga
     1801 ggctttcagc ccgttattat ttattatttt gcctatcatg atacttggag aagaattgtt
     1861 ctcagtgtat tttctcgcta ttttttctag taagttcagc attcctgtcg ctagtttctt
     1921 gtcagcaatc atctttggat ttatccatta cagtacttat gataatggaa atattttaca
     1981 cactgtagca cacatccttt tgatacaagg ggtggcaaga ttgttattca accaagctgc
     2041 cattaaaagc aactcgatca ttacaagttg ggcagttcat gtgatatttg atttcacagc
     2101 tatattgcta gtcgttttat tctcctaaat ttttatagaa ataatacatg ctctattgct
     2161 aagaaagcat actttatttc ctttaaacaa aaagacagat gtatcaatgg attaaaa
//
LOCUS       DAYYBH010000158         2205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000158 DAYYBH010000000
VERSION     DAYYBH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.165"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHAUG_002894"
     CDS             complement(<1..914)
                     /locus_tag="ACHAUG_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8481917.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACHAUG_002895"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACHAUG_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8481918.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACHAUG_002896"
     CDS             2006..2176
                     /locus_tag="ACHAUG_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481919.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          747 a          384 c          421 g          653 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtata
//
LOCUS       DAYYBH010000159         2139 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000159 DAYYBH010000000
VERSION     DAYYBH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.166"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACHAUG_002897"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACHAUG_002897"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8481920.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1708..2025
                     /locus_tag="ACHAUG_002898"
     CDS             1708..2025
                     /locus_tag="ACHAUG_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8481921.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
BASE COUNT          759 a          260 c          345 g          775 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc ttcttactat gaataagata ataaaatttg tatttgtcct
     1501 ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatcttttta
     1621 cgaaaaacat cacaaggaaa aacttaacta tggtattatc taaacataat tttttcggta
     1681 tttaatattt aattaggagg gaatattttg cttttttata gtaattttat cttaatagta
     1741 gcgatattat tacttctgaa tatatggatt tttgatagga gtagaaatgc aagtataggt
     1801 tttagaacta aacgaagtct gtcttctaaa aaaaattggg tatactcaca aactattttt
     1861 tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt gaatattata
     1921 gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc aattattaca
     1981 caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa ctagtgatag
     2041 ttttggtgat agtgatatta aaaatcagaa aagatagaaa aagcaggaga tagataaaat
     2101 gctgatattt cagcattttt taagagtata aaagagaaa
//
LOCUS       DAYYBH010000160         2099 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000160 DAYYBH010000000
VERSION     DAYYBH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.167"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACHAUG_002899"
     CDS             85..624
                     /locus_tag="ACHAUG_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481922.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            897..2069
                     /locus_tag="ACHAUG_002900"
     CDS             897..2069
                     /locus_tag="ACHAUG_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFM8481923.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          742 a          344 c          416 g          597 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttgata aagtccatat aattgtgtaa aagataaaag gccatgtaac
      841 agccctttta cggtacaatg tttttaacca caaaaacata cccaggagga cgttacatga
      901 cccaagtaca ttttacactg aacaacgaag aggttcaaag tattattgaa cattcggtaa
      961 aagatgatgt ttctaaaaat attttaacca ctgttttcaa ccaattgatg gaaaatcaac
     1021 gaacagaata tattcaagcc gatgactatg aacgttcaga aagtcgtcag agtcaaagaa
     1081 atggctatta tgagcgagac tttacgactc gtgtgggtac actcgaatta aaagtgccta
     1141 gaacacgtga tggtgaattt tcaccgacgg tgtttgagcg ttatcagcga aatgaaaagg
     1201 cactgctcgc ttcaatgctt gagatgtatg tttcgggcgt ttcgacacgt aaagtttcaa
     1261 agattgttga agagctatgt ggaaaatctg tatcgaaatc ttttgtttct agcctgactg
     1321 agcagttaga cccgatggtc aacgaatggc agaaccgttc actctcaggt acgaattatc
     1381 cttatctgat gactgatgtt ctctacataa aagtccgtga ggaccatcga gtgctttcta
     1441 aaagctgcca tattgcgatc gggataacag aaggtggcga ccgtgaaatc attggcttca
     1501 tgattcaaaa tgaagaaagt gatgacacat ggtccatctt ctttgaatac ttaaaagaac
     1561 gcggcctaaa ggggacagaa ctcatcattt ctgatgccca taaaggccta gtgtctgcga
     1621 ttcgtaagtc atttaccaac gcaagttggc agagatgcca ggtccatttt ttaagaaaca
     1681 tcttcagttc cattccaaaa aagaattcaa aaccgtttag agaagcagta aaagcgatct
     1741 ttaagtttac ggatattgaa ctcgctcgaa cagctaagaa tgccttagtc ggtgaatata
     1801 tcgaccagcc taaatataca aaagcctgcg aaatattgga taatggcttc gaagatgcct
     1861 ttcaatacac ggttatcgga aatagtcata atcggctaaa aagcaccaac cttcttgaac
     1921 gactgaacca ggaagtccgc agaagagaaa agattattcg gatttttccc aaccgaacgt
     1981 ctgccaatcg attaattgga gctgtcctta tggaccttca tgacgaatgg ctcagttcta
     2041 caagaaaata tattaagttt gatcaatgag agaccggtaa aacattgtat agtatttta
//
LOCUS       DAYYBH010000161         2096 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000161 DAYYBH010000000
VERSION     DAYYBH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.168"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..304)
                     /locus_tag="ACHAUG_002901"
                     /pseudo
     CDS             complement(18..304)
                     /locus_tag="ACHAUG_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(294..623)
                     /locus_tag="ACHAUG_002902"
     CDS             complement(294..623)
                     /locus_tag="ACHAUG_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8481924.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(700..972)
                     /locus_tag="ACHAUG_002903"
     CDS             complement(700..972)
                     /locus_tag="ACHAUG_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8481925.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(972..1235)
                     /locus_tag="ACHAUG_002904"
     CDS             complement(972..1235)
                     /locus_tag="ACHAUG_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8481926.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1469..2096
                     /locus_tag="ACHAUG_002905"
                     /pseudo
     CDS             1469..2096
                     /locus_tag="ACHAUG_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
BASE COUNT          675 a          402 c          288 g          731 t
ORIGIN      
        1 agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt ttcatgcgtt
       61 ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac tctatatgtt
      121 gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt tttttgttca
      181 cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt ttctttcaat
      241 ggcttctctt ttagcttttt aaatacttaa tgacatgttt tgaaggaacg atttcataag
      301 ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag tgtatcttca
      361 gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc tacaagaatc
      421 tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct aaaatcataa
      481 aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa ctttgcttca
      541 acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat tgaattgcct
      601 tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt aattacttag
      661 taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact cgtaatgcgt
      721 ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttataaa caacccggtg
      781 ttgtctattc aatcttctag aatacctacc ttcatgtgta ggttttaact tctcaaatga
      841 ttgggtaggg agatagggat cttcctttaa agtctgaata accttctcaa attgaggttt
      901 caaattagac tgtttgattt tctttaaatc agctttagcc gagtttttaa tcatcacaga
      961 atagttattc attataagtt atcccaatca atttcatcaa cattagtaaa tccactatca
     1021 tctttttctc gcttacgaac aacacccaaa gtgcctattt gctcaagtaa tagcgtttct
     1081 ttgattgctt tccaatcatc caaaccaata agcaccgcat tattttcact gcgctcacta
     1141 atgatttcaa tttccgtatg attctcattt acatctttca acaacttata aaaatttttc
     1201 cgtgctgagc taggatttaa gactgttttt gccatatcta ctccccctct aatttatatc
     1261 tccattctaa cgtacttaat taagtacgta aattcgattg ttttgtttaa attggagtac
     1321 caaaacagca ttgtaaagtg ttatccgttt cctttatacc cgtggtgcta gttaattttt
     1381 tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat agagtagtct
     1441 caaaactccc aagaaatgag gactacatat gcgttcatca aaatatactg aacattatac
     1501 agatactttt ataacattca aggaaattat gagaactgtt tctaacatat accacaattg
     1561 tgtaccagat aagattaaaa accgaagaaa tactgatcaa ctgaagtaac gcgatacagt
     1621 gattattgct tgtgttatat ggggcataat taatggctat actagccaaa gagccacgta
     1681 tagagcggtt tgttccgtct tatttcctaa tggtgacttt cctagtagga gtcgattcac
     1741 tcgcttaagt tcaaacttag cttacactat caagattatt cgatactttt tcatcaagaa
     1801 gctcaccaaa ggtgaactag tcggaattat agatagtttt cctagtccat tatgtaaacc
     1861 agttagaaat agacaagcaa aactattgaa tcaaatagcc aaagttggct ataactcaac
     1921 aaaaaaatct tatttttatg gtcttaaaat tcatatgatc gttactaaga ctggctttcc
     1981 gattacttac tcaatcacga atccaggtgt ccatgacgtg aaagtgttag agactttatc
     2041 ggaagaagcg aatcttccca atatcctagg ggacaaagga tatattagcc ataaaa
//
LOCUS       DAYYBH010000162         2019 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000162 DAYYBH010000000
VERSION     DAYYBH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.169"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..570
                     /locus_tag="ACHAUG_002906"
     CDS             100..570
                     /locus_tag="ACHAUG_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8481927.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="ACHAUG_002907"
     CDS             792..1895
                     /locus_tag="ACHAUG_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8481928.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DAYYBH010000163         2003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000163 DAYYBH010000000
VERSION     DAYYBH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.170"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACHAUG_002908"
     CDS             <1..378
                     /locus_tag="ACHAUG_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8481929.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACHAUG_002909"
     CDS             426..1826
                     /locus_tag="ACHAUG_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8481930.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAYYBH010000164         1979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000164 DAYYBH010000000
VERSION     DAYYBH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.171"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACHAUG_002910"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACHAUG_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACHAUG_002911"
     CDS             complement(728..1744)
                     /locus_tag="ACHAUG_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8481931.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAYYBH010000165         1948 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000165 DAYYBH010000000
VERSION     DAYYBH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.172"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..482
                     /locus_tag="ACHAUG_002912"
     CDS             180..482
                     /locus_tag="ACHAUG_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8481932.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(998..1071)
                     /locus_tag="ACHAUG_002913"
     tRNA            complement(998..1071)
                     /locus_tag="ACHAUG_002913"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1035..1037),aa:Ile,seq:cat)
     gene            complement(1626..1940)
                     /locus_tag="ACHAUG_002914"
     CDS             complement(1626..1940)
                     /locus_tag="ACHAUG_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481933.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          708 a          290 c          301 g          649 t
ORIGIN      
        1 tttataaggg tttgagcatt ttttattttc gcagattttc gttaaatgaa tgaaaatggc
       61 attcaaaata gcattcaaaa agaacacttt ttaatatata tgttccatcc atattatcaa
      121 tgatattcct actttacaaa aagaacatta gttcgtatac tctttttgag gtgaacatta
      181 tgttgatgga aggaaaaaca caattatggt ttaaatttga tccttcgaat agatttgtca
      241 aagattttta taaggtgtgg gattcagaag ttttcttttt agcaatcgaa gatagcttat
      301 taatcaatct ctattactct aataagaact actttaaaat tccggcaaca aaaaccagaa
      361 tgaagaaaga tgtgtatttt ttgtttgata tcgtgactga tgtgccagac gcacggagcg
      421 atcatcggcg ttatgactat ttgaagtata ctttcgttga tccagaaagg tataaagatt
      481 aaagtaggct acctaaaaaa ggtagcccgg aacggatttt atcaccatac ttataaaagg
      541 gatatttttt aagttagtat taagattgtg taatatgatg atatctatat tttatagtat
      601 cagtgctata aaatcaaaaa taagtcacta attaactacc actccaatta taagtctttt
      661 tcccattatt ttttacagtt atatggtatg ctttttaatg gcttcaaata taaaagagtt
      721 taaagcgtaa cacacttatg gggaagtggt ttttggggaa cgctttaaac tcttctttat
      781 tattatctca caatataacc caaatgtctt tccatttaaa aatcaaagta aaacttttca
      841 aatatacaga agtataacta tgtgaaacat cctttcatta atccataaaa ggatacataa
      901 aaaagccact catttgagtg gcaatgaaga aaagctttag cttgtataat actcttcaaa
      961 aaattctaac acagaacgat tcaattggct acattaatgt accctgtagg actcgaacct
     1021 acgaccggac ggttatgagc cgtctgctct gaccaactga gctaagggta gtggttgttg
     1081 ccacataaag ccataaacaa tcaaccagta gaatgcgtgg caacaaacct gttatcgcat
     1141 atcttggagt gtgactattt atgagtgata gtgaagatat gcgtctccat atatgtatgg
     1201 ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt attttggtca
     1261 aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc aaaaaaacat
     1321 tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg tttacaaaaa
     1381 gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa ggaaaaacac
     1441 aattatggtt taagcatgtt ccttcaaata agttttttaa ttataaactc tagagaaatt
     1501 aattcatcct aacataaaat aattgttcac tcaaaagttt ttctagaaca attaaaacag
     1561 caggcttcac attcgctata catttgtaag aagtaataat tcctgtacta aaaggcataa
     1621 ttgctttaat tattttttga cttctcttca aacacaagaa gtagttgtgt aataattgca
     1681 gaaataagga ttccaataat agatataaaa tttgacatgg aaacgtctat aacattaaaa
     1741 gaataaagtg ttgaagaaag taaactgatt gaaataaccc ctccgtaaaa aatagtctgt
     1801 gaatataccc actttttttc agaagacgta cttcgtttgg ttctaaaacc tatccctgca
     1861 tttctactct tatcaaaaaa ccaaatattt agaagtaata atattgctat tattaagata
     1921 aaattactgt aaaaatccaa aagctatc
//
LOCUS       DAYYBH010000166         1927 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000166 DAYYBH010000000
VERSION     DAYYBH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.173"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..730)
                     /locus_tag="ACHAUG_002915"
     CDS             complement(29..730)
                     /locus_tag="ACHAUG_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8481934.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(746..1186)
                     /locus_tag="ACHAUG_002916"
     CDS             complement(746..1186)
                     /locus_tag="ACHAUG_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8481935.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1186..1761)
                     /locus_tag="ACHAUG_002917"
     CDS             complement(1186..1761)
                     /locus_tag="ACHAUG_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8481936.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          553 a          409 c          296 g          669 t
ORIGIN      
        1 ttctttcccg tcgtttggtt ttttattttt agcctaattc ttgacctttt gcatgctggt
       61 tatccgtaaa atattttcca gcaggctttc cttccaattc agctaatttt tcttggatct
      121 gacggatcac cttttttgca tcttctggat tttctacaaa atcgaattta tccccatcca
      181 cttgtatttt aggacataca tcaaaatctt cataccattg attatagcgg gtgttcaacg
      241 tttcataata ggtatataac gtctcatcgc tttctagctg ttcatagtca cgcccacgtt
      301 ttttgatacg ttctagcatc gtgtccaaag aaactttgat atggaccatc aaatcaggcc
      361 gtttttttgg agcaacatca tctaactcgt ttagcatcgt atctaataag ctatcgtact
      421 gctgtacttc gatatccgtt acccgaccta aatcagcgtt tagatggaac aacaggctat
      481 cttcatagat ggatcggtct agcacattgt catcgttcac taaagcattt ttcattgcta
      541 aaaatcgctt gttcaaaaag aagatttgca ataaaaaagc gtactgctca ggatttgaat
      601 aaaagagcgg caatacttcg ttatcttcga cattttcata aaatggttta gaatcaatct
      661 cttttgcaag catatccgtc aaactgctct tacctgcacc aatcgttcct gaaatcaaaa
      721 tcactgacat acaacccttc ctcttctata agtactcgcc tacgtaagtt tcaacaggca
      781 atgtttcaaa atcatattca cgtacagctt ggtctgattt taaatacgta tggagacttt
      841 gtgtaatcat caagttcgta ggtaccgtct cttcttgtac aatgatcatc ggttttttca
      901 acatagttgc cacacctaat tcgtaagccg ttcctggatc gattgtttga tgctcaaagt
      961 caaggatagc aacaacaaat tctgcatttg tcagttcttc catatctttt tcgtatactt
     1021 cctgcgccca tccagcgctg aatagttcat aattgctttc ttggtgatga cgtggagagt
     1081 agaaacttgt gactgttttg ttttcctcca acgctttttc gatacgactt actcgatcga
     1141 tttgctcctc tgaaaaaaat ggtcctgcta aatatatttg actcattatt ttgcctcctt
     1201 caataataac tgatttttaa tagatactgc tcgtaattca ttgaccagtt ccaatggatt
     1261 gttggaaaag attacttgat ccaaccattt tttatccata aaatcctctt ttgcaaaagt
     1321 ttctaataat tgttgcaagc cgctgtaata accgccaata ttcaatattc cgattggctt
     1381 gtcgggcgtc agaccgattg ctatccaaga aagaagttgt gagaattcct ccaatgtacc
     1441 tagacctcca gggaaaacga ggacagcatc tcccttttca aataataata cctttctttc
     1501 atcgatcgtt tctgtaggaa taaatgtagt aacatctttt ccaggcaatt cagtttcaaa
     1561 aagcccattc ggatagatac ctgtgacttt tccgttattt tccagtactg ttttactaat
     1621 cactcccata atcccggagt atgaaccacc gtaaaccagt tcgatttctt cttgtgccat
     1681 gtattttcct aacgtttgcg caataaattt gtaactttct ttattaccaa aacgggaacc
     1741 gcaaaaaaca gtaagtttca taaaaaaaca ctccctaact caaaagtcta tcttcattct
     1801 acacgtgttt ttttaaaata aaagactaga ttttgtagtt ttttagtggt atataggata
     1861 gacgccacta tatatagtat ttagaaaaaa atcatttttc agccatttta tagataaaac
     1921 aaaaata
//
LOCUS       DAYYBH010000167         1924 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000167 DAYYBH010000000
VERSION     DAYYBH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.174"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1227)
                     /locus_tag="ACHAUG_002918"
     CDS             complement(<1..1227)
                     /locus_tag="ACHAUG_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFM8481937.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLY"
     gene            complement(1224..1877)
                     /locus_tag="ACHAUG_002919"
     CDS             complement(1224..1877)
                     /locus_tag="ACHAUG_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8481938.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          634 a          296 c          152 g          842 t
ORIGIN      
        1 atataaacct aatccattac tattttcctt atttaaatca aatttaacat caaatatttt
       61 atccatattc gaaattttat tgttaccgta tgaattctct atatataacc aatcattaac
      121 tatcccaata ttaattaccc catttacatc agtatatttt accgcattac taatcaaatt
      181 agatagaata atctttaaag ctgtttttcc tatataaaca ttctcgtcta atatataatt
      241 attgacagtt attttctttt gatttgctaa tatttcatac ttttttaata tgtcttctaa
      301 tgtatcattt attttcaaat attcttcatc attattcaaa ttttctatag aatgaactga
      361 taatatttga gaaatatttt ttgttaaact atcaacgatt tcaatacatt cattaatata
      421 aatatctctt tctttatatt ttccaatatt gtatttcatg ttttccagta ttattttaag
      481 actagcaaga ggggttttaa gttcatggga tgcaccttta aaaaagtcat attttaactt
      541 ttctaatttt aaaatttctt tatttttaaa ttccaaatcg tcaattgttc ttaataaagt
      601 agaatacaaa tcattaattt gttgttttaa ttctccaact tcatcattag agctaacttt
      661 taaactcatt tttttatcaa gtttcatcat tttatcagta acaattttta tttcttgtat
      721 attatttttt attgattttg cataaattaa agaaataata gcagaaaata aaattgatat
      781 taataaagaa tatggtaaaa atttaaggct taaatctttc gcatcttttt gcatatcagc
      841 tgtagaaaca aattgtagat ttgttttttt accatcatta agctttattt ctctttcttc
      901 aattatcaaa gaattactat cactttctaa attaacattt atattatctt tgatttgtat
      961 ttcattctta ttattttttt cttttatgaa tacttttatt tcactactct tggaataaaa
     1021 ctccaaagtt tgttctatat attttatttc ttttccattc atattactag aaatttcatt
     1081 tgccatatta tgaatctttt cttttctcgt ctccaaatat gttttaggaa agataaaaaa
     1141 aattaatgaa tgaactaata taattattat tccaagaaca gaaaatattt gtatgaacat
     1201 ttttggaaat atctttaatt ttttcatttt ctctccaatt tatatcctac atttcttata
     1261 gtagttatac agtctaattg taattttttt ctaagttctt ttatatatac atctattact
     1321 cgatcataag gagtttcttc gctatctttc catacataat caataatttg cattcttgtt
     1381 aacacttgtc cattattatc caataaacat tttaatactt ctagttcttt tgcatttaca
     1441 tctatttctt caccatttat tttagctgta tagctattaa aattaaccga tagatcctta
     1501 tattcaaact tctctaaatg tccataattc ttcttaatta aagaatctat tctagctttt
     1561 aagaccggca atgaaaatgg cttttctaca tatccgtcta ctaaattagt aaatgcatca
     1621 attttatatt cttcatcact aaatgcagtc aagattagaa ttggtaaatt gcttttcttt
     1681 ctaatttctt tcaacacttc taaaccattt ataaaaggta tctgaatgtc taagataacc
     1741 aaatttatat cgctattaaa ttttgacaag gcttctcttc catctttagc ttgaataaca
     1801 gtatatccaa attctgaaag atattcactt attccctctc ttatcatatt atcatcttca
     1861 acagttaata ttttcatata tacctccact taagcctata cactagatat tatacagcat
     1921 atta
//
LOCUS       DAYYBH010000168         1852 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000168 DAYYBH010000000
VERSION     DAYYBH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.175"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..1245)
                     /locus_tag="ACHAUG_002920"
     CDS             complement(226..1245)
                     /locus_tag="ACHAUG_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8481939.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1242..1466)
                     /locus_tag="ACHAUG_002921"
     CDS             complement(1242..1466)
                     /locus_tag="ACHAUG_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8481940.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1463..1756)
                     /locus_tag="ACHAUG_002922"
     CDS             complement(1463..1756)
                     /locus_tag="ACHAUG_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481941.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          522 a          381 c          320 g          629 t
ORIGIN      
        1 atgagccgtc tgctctgacc aactgagcta agggtactgg ttgttgccac ataaagccat
       61 aaacaatcaa ccagtagaat gtgtggcaac aaacctgtta tcgcatagct tggagtgtga
      121 ctattatggg tgatagtgaa gatatgcgat aacatcacta ttttatcgaa tgatttttat
      181 agttgtcaat atagttatgt aatgctcctc aacgaggagc tatttttatc gtttaggaat
      241 atttaaatac caacgtttgt catggaaatc ttgcgcaccg cctttagtgt ttccctctgg
      301 atcattcgtt gcccgcatca tgacatagac tttcttatta gggaagttac gcatgttaaa
      361 agatacatga tagccaacat ttccagaagt attataagct tgatttacat ctgatctata
      421 aattccatca gctcttactc gagctaattc tttcccagta ttgtaatcca taatgaagat
      481 atactcgtat ttatagttag caatgtgcca tccagctaca tgcaaatttg cattttcgat
      541 ttctccaaac tgatcaatgt gggcgtaatt cgttccatct gtcagtgtag gattagctgc
      601 accagctcgt gttggatcaa tgacaggctt gttttcagaa gttgttggat tttcatcggt
      661 aaatccatga gctaaatcat atgctaattt ttctttactt acgcccattt cagaaagata
      721 accgtaagga tctgtatgat cgccccagat gttttgtgtt acccataaat gcgatttgat
      781 tcccggttgg ttataaggag tgtccaacgt taatggaata ccatatttca ttgctgaatc
      841 tctagccaat tcaacgtatg ccttgtagtt tttctcaaac gttgctttat catgtgtgtg
      901 ttgtaactca atctgcacag gactgttggc attagcatac gaaccagcac cgtactgtac
      961 ataaccaggt tgaccgactt gataaacaat tccgccgtct cccacaatat aagcagtata
     1021 agcgctagtc catgaacgtt gcatatactg cgcttcattg cgtcctgttg ctgtttcatt
     1081 agccgtttca tgcagtaaaa tatactgatt atttgctact tgtgagctac cttcatttgc
     1141 gcccaaatta aattcattgt tgatagtata ggcaaatccg ttaattggca ataaaaaaag
     1201 agccgttaac aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct
     1261 tcgctttcag ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa
     1321 aacgcgccaa aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct
     1381 ttaccaacta accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt
     1441 agtacttcga acgttttatt attcattttc ttctctccct aaacaaagtt ttgatttgtt
     1501 gcgtgtgttc caccaatttt tctgtatgtg tatctaatct ttcatcgtgt ttctttagtt
     1561 cttcatgaat catcaatcga tctgatttgc tcgattctaa atctttagtc agcaaatcta
     1621 aattgtggct tacttttgaa agagtctcag taattttcga gaaagatgca gcaatcggtc
     1681 taattactaa taaaatcaaa gaaacgatag cggttattga tcctgctatc attccccatt
     1741 cccctaaatt aatcatgtga caactccttt accttaaata aaaacgcatc aattaagatg
     1801 cgctcttatc tttattaatg attttatctg cttcttcgtc tgtaatgcat aa
//
LOCUS       DAYYBH010000169         1817 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000169 DAYYBH010000000
VERSION     DAYYBH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.176"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..233
                     /locus_tag="ACHAUG_002923"
     CDS             <1..233
                     /locus_tag="ACHAUG_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481942.1"
                     /translation="GDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIFMIVIWFLNE
                     RSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(485..1057)
                     /locus_tag="ACHAUG_002924"
     CDS             complement(485..1057)
                     /locus_tag="ACHAUG_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8481943.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1073..1678)
                     /gene="fic"
                     /locus_tag="ACHAUG_002925"
     CDS             complement(1073..1678)
                     /gene="fic"
                     /locus_tag="ACHAUG_002925"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8481944.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          535 a          367 c          270 g          645 t
ORIGIN      
        1 aaggagactt gaatgccatt attactttaa tcgtagttat ggtaaaatca ataggtaata
       61 tcacaatagg aatgctgttt attatattag cagcagtgct gatttttatg atagttattt
      121 ggttcttaaa tgaaagatct aaagacaatc aacaccgtag ggatatggag ataaaatcaa
      181 tagagtcaat agattattta tcgataataa cactaaaaaa gccacgttcc taaacgtgac
      241 ttacgctgaa cgatatgtgt tgtttggtag acgaacaaat cgttcattat aaatatatga
      301 tggtatccta atatagtaag cgtggctagc atacctttaa ttactttacc ttttacagtt
      361 accatacaca ttttttgagc agaaatcaag taaataaaaa aatattatat ttcacaacat
      421 gctaatagac tcatatctag aacgcattaa gtatttacct gtctggttcg ctgagacttc
      481 ttttttaact ttcttcaatt ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg
      541 ttatagcttc aacttcctta tacgatttac ctgaagtaag cagttcatat gcgtgcctct
      601 tcttcggtgt gattgttgct ttcggacgtc cttctctgaa attaggatta ttctttcttg
      661 caaacatttt tccttcttgg gtacgttcca caatcatatc acgttccatt tctgctacac
      721 tcaacaacgt ccgtgtcacc attcgcccca tggacgtatt ttcaatcgtt cctagattca
      781 aaaccttgat cgttacgtta tcatcgagta aaggttcaat gatctctaat gcttctctcg
      841 tgttacgagc aaatcgatca agcttcgtaa caactatttc atctccaggc ttcagaccag
      901 ccaacagctg attgaatttt ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa
      961 tattcttctt ctctacgccg ttctcaagca actgttgaat ttgttctttt gtactttgag
     1021 aagcggttga gacacgtgca tatccatatt tcgtcatttg gtccacctac ttttacaaat
     1081 catctatcgt atactcagaa tacccttcat agaaatagct gacatcgatt cctttcatat
     1141 ataactcgcg atcgtcaatt ttatcagtca gagcattgga aattagatag cgtatttcca
     1201 aatcgtttat gggacttcgc tccattgcag aaagatagtc tgctttatcg attaagttcc
     1261 aatctacaac tttttgcaat tcttccttta aaatgagatc cagccaaatt ctagtacttc
     1321 ggccatttcc ttctcgaaat gggtgagcga tattcatctc cacatatttc ttgataatct
     1381 cttcaaaaga agtttgtggc atctgatcaa tatgctgtaa cgaatgttct aaatacataa
     1441 ctggtgcaaa tcgaaaattt ccttttgcaa tattgaccga tcggattttt ccggcaaaat
     1501 cgtaaatatc cgaaaataga aattcatgaa tttcagataa acctttaaat gtaccaactt
     1561 cgatttcatt tatttttcca caatcgtaca attctttcgc tttttgttta ctgagttttt
     1621 cttcaacctt agccaactca acttgattgg ttaatcccaa tttattctct aaaatcatgc
     1681 gaaatctcct taactttatg acataagttt atgaaacact ctcaacccca ttatatcgcg
     1741 attttgttta tgtgtcaaaa actattaagt ttctgacccc cgatagattt ttcaagtgtc
     1801 tctattttgc acagtct
//
LOCUS       DAYYBH010000170         1739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000170 DAYYBH010000000
VERSION     DAYYBH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.177"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACHAUG_002926"
     CDS             <1..189
                     /locus_tag="ACHAUG_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8481945.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="ACHAUG_002927"
     CDS             complement(1055..1585)
                     /locus_tag="ACHAUG_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFM8481946.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..>1739)
                     /locus_tag="ACHAUG_002928"
     CDS             complement(1582..>1739)
                     /locus_tag="ACHAUG_002928"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8481947.1"
                     /translation="AFYFSAIIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILI
                     TKRDGER"
BASE COUNT          556 a          342 c          206 g          635 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaa
//
LOCUS       DAYYBH010000171         1731 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000171 DAYYBH010000000
VERSION     DAYYBH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.178"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHAUG_002929"
     CDS             <1..156
                     /locus_tag="ACHAUG_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFM8481948.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACHAUG_002930"
     CDS             169..1077
                     /locus_tag="ACHAUG_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8481949.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1697
                     /locus_tag="ACHAUG_002931"
     CDS             1077..1697
                     /locus_tag="ACHAUG_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481950.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          613 a          251 c          395 g          472 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaga atacagaggt tatggaagcg actatagttg aaaatggtct tccctacgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgtaggtg ggaaatatcc agtccaatta
      361 gaaacaaaaa ttgtggatgc taaaaaaggg aaaattaact atatttttga taaatattcc
      421 atgcagtgtt tacatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ttattttgtc attaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aatcgaagat ttaattgcgg atatgacgga ctttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgaacgttgg
      661 agacaagaac aggaacacag tttccaagat tggagagaag gacaagaatc agactatttg
      721 gcatggtttg aatcaatcaa ggatatttta aagacgattg atccaggtgg agtcatgtta
      781 gccgaactaa cggatgcgcg agtagatatt caaggagttc gtcatgcgtc tatttcagag
      841 cgtttgctgg cagatatgga ctatttatat cagaaattgc gagcaacgct tttcacgatt
      901 gaatacgggg agattgaagt gactgatatt ttgcaggatg atctcttttc agataatcat
      961 gaagtcgaaa aagttggaac tgtagcattc ccgattgaag aaggagcctt gatcatcgca
     1021 accgttgatg atccaaagca aaatgtgttc actcttgaga aagttggggt gatctaatgg
     1081 ctaaaactaa acgaatgatg gaaacggatg aaaaaacggg tgtacaacgc caattctttc
     1141 caatcacaca tgcttccgca gttcttgggt tagaagaaat aatagcagga gaagcaacag
     1201 ttttatctgt taatggaaaa attggagccg tcgtcattac taaagaagat ttgggattag
     1261 agaatgttct cacagaatta ccctatgcaa gtgaagaaga tgacggcatt atcacggcag
     1321 aaatgtatca aaaaatttta aacagtggag aaggtgacta cgtgttgcca gttgccacta
     1381 tcgaccgttt gggtggcata aaagttggtg aattattgac gattgatgag acagggaaag
     1441 tctctgcagt cagacaatct gatgtcaatt tttcgctgga gttaaaagaa aaactcgatt
     1501 cactgaaaaa ttatactgct ggagaaaaca tcactattga tgaagatgga acaattagtt
     1561 ctacaggtgg tagtgggact ggtggtgtca atcagtcata tgtagatcaa aaattccaag
     1621 aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt acttttgaaa
     1681 aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc a
//
LOCUS       DAYYBH010000172         1728 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000172 DAYYBH010000000
VERSION     DAYYBH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.179"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHAUG_002932"
     CDS             <1..156
                     /locus_tag="ACHAUG_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFM8481951.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACHAUG_002933"
     CDS             169..1077
                     /locus_tag="ACHAUG_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8481952.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1694
                     /locus_tag="ACHAUG_002934"
     CDS             1077..1694
                     /locus_tag="ACHAUG_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481953.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          629 a          231 c          368 g          500 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaaa acactgaagt tatggaagct acaattgtcg aaaatggtct tccatatgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgttggcg gaaaatatcc agtccaatta
      361 gaaacaagaa ttgtggatgc taaaaaaggg aaaatcaaat atatttttga taaatattcc
      421 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      661 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      721 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      781 gccgaattaa tggatgcacg tgtagatctt caaggtgtac gccatgaatc gatttctgaa
      841 cgtttcctat ccgatctaaa ttatttgtat caaaaaatga aagcagcact tttcacgatt
      901 gaatacggtg aaattgaagt gactgatatt cttcaagacg atctcttttc agataatcac
      961 gaagtcgaaa aaattgaaac agtagaattt ccaattgaag aaggagcttt gattattgca
     1021 actgttgatg atccaaaaca gaatgttttc actcttgaga aagttggggt gatttgatgg
     1081 ttaaagtaaa gagaatgatg gagactgatg aatctggtgt taaacgtcaa tttatgccaa
     1141 ttacacatgt ttctgcagtt ttaggcttag aaaaaatgat atcaggacaa tcaaaagttc
     1201 tttcggttaa tggtaaatat ggtgcagtca ttttaacgaa agctgattta ggattagaga
     1261 atgcaattac tgaactacct tatgcaagcg agacaagcga tggtattctt actgctgaaa
     1321 tgtttcaaaa gatcgtaaat ggagaaggtg gaacatatat tttgccgatt gctactcctg
     1381 aacaattagg aggtataaaa gttggggaac tcttagaagt tactgaagaa ggtgttttat
     1441 ctgcaactaa gcaaacagac ttcaatttct ctgaagaatt aaagggaaaa ttagaatctc
     1501 taaaaatatt aaaagcaggt gcaaatatat caattgcaga agatggaaca attagttcta
     1561 caggtggtag tgggactggt ggtgttaatc agtcatatgt agatcaaaaa ttccaagaag
     1621 ctgtaaatca agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa
     1681 tcggggaggt atagaaaatg acagatatcg taaaaataaa acaaagcg
//
LOCUS       DAYYBH010000173         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000173 DAYYBH010000000
VERSION     DAYYBH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.180"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHAUG_002935"
     CDS             131..505
                     /locus_tag="ACHAUG_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8481954.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHAUG_002936"
     CDS             complement(619..1248)
                     /locus_tag="ACHAUG_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8481955.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHAUG_002937"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHAUG_002937"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAYYBH010000174         1584 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000174 DAYYBH010000000
VERSION     DAYYBH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.181"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACHAUG_002938"
     CDS             288..695
                     /locus_tag="ACHAUG_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481956.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACHAUG_002939"
     CDS             709..1110
                     /locus_tag="ACHAUG_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481957.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACHAUG_002940"
     CDS             1112..1483
                     /locus_tag="ACHAUG_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481958.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          595 a          197 c          274 g          518 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgct
//
LOCUS       DAYYBH010000175         1536 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000175 DAYYBH010000000
VERSION     DAYYBH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.182"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACHAUG_002941"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACHAUG_002941"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAYYBH010000176         1428 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000176 DAYYBH010000000
VERSION     DAYYBH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.184"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(236..535)
                     /locus_tag="ACHAUG_002942"
     CDS             complement(236..535)
                     /locus_tag="ACHAUG_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8481959.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(541..771)
                     /locus_tag="ACHAUG_002943"
     CDS             complement(541..771)
                     /locus_tag="ACHAUG_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481960.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1030..1143)
                     /locus_tag="ACHAUG_002944"
                     /pseudo
     CDS             complement(1030..1143)
                     /locus_tag="ACHAUG_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          506 a          229 c          206 g          487 t
ORIGIN      
        1 ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa gtgagaaaaa
       61 aggcttataa ttggagtggt agttaattag tgacttattt ttgattttat agcactgata
      121 ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa aaatatctcc
      181 tttcataagt atggtgataa aatccgttcc gggctacctt tttaggtagc ctactttaat
      241 ctttgtatct ttctggatca acgaaagtat actttatata gtcataacgc cgatgatcgc
      301 ttcgagcgtc tagcacatca gtcacgatat caaacaaaaa atatacgtct ttcttcattc
      361 tagttttcgc agcagggatt ttaaagtagt tcttattaga atagtagaga ttaattaata
      421 agctatcttc gattgctaaa aagaaaactt ctgaatccca caccttataa aaatctttga
      481 caaatctatt cgaaggatca aatttaaacc ataattgcgt ctcattaaaa agcataacca
      541 ttactccgat ctatttttaa acttagtctc tacctctaat atatatcgag tttttatttt
      601 gccttcagag aatactgttt ctttttttgc agttacaggt tgtttatttt cggaaaaagc
      661 taatatagct aaaattgaaa catccatctg gaatttgtct ttttcgttgc tttgctcata
      721 aaaatctgca tattcatcac taatattttt tctaataaat tcttccatca taaagatcac
      781 ctcgaaaaga gtatacgaac aaacgttctt tttgtaaagg tggaatttat tatactaaat
      841 aaataggtga acaagtactt gtgccaaatt atgtgccaaa aaaaatcgaa tttaataaaa
      901 cacaaacaaa aagaaatcct attatgataa catttcttat aataacaaac acaacagaag
      961 acgtgtaata gttagtcagg aacgtacaaa taacccctgt atcctttgcg gtacaggggg
     1021 ttattttgta taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
     1081 gctaagtata tttactcctt gataaagtaa aatagatgca tgcgtatgtc ttagtccatc
     1141 aataaacagt cgaagagaaa atagggaaaa attgattact aaagaaaaac ttactaggtc
     1201 gacggtgtag ttgattttca tacttcaata gattcgaatt aaacgatttc gattggctta
     1261 taccagtcct atccaacgaa agatatatcg ttcttaactc tagcggtata acttgaagct
     1321 ttacgtaaag ccaatgggaa tgaactggag atgacttttt attattttta gtaatagctg
     1381 atgggttaat acttacaact ttccttttca aagtaccata taaaaaaa
//
LOCUS       DAYYBH010000177         1417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000177 DAYYBH010000000
VERSION     DAYYBH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.185"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACHAUG_002945"
     CDS             complement(150..1337)
                     /locus_tag="ACHAUG_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8481961.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAYYBH010000178         1378 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000178 DAYYBH010000000
VERSION     DAYYBH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.186"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..361)
                     /locus_tag="ACHAUG_002946"
     CDS             complement(<1..361)
                     /locus_tag="ACHAUG_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8481962.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREV"
     gene            complement(362..1270)
                     /locus_tag="ACHAUG_002947"
     CDS             complement(362..1270)
                     /locus_tag="ACHAUG_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFM8481963.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(1270..>1378)
                     /locus_tag="ACHAUG_002948"
     CDS             complement(1270..>1378)
                     /locus_tag="ACHAUG_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8481964.1"
                     /translation="EIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          457 a          242 c          146 g          533 t
ORIGIN      
        1 aaacttcacg taaaggtgaa gtcaaatcta aatctataat taaatcaaat atattatctt
       61 ctctttctag ttgtaagaat gaatcacgta tcacttcaat tttccctaca gtatctgtag
      121 ctaattcagg tttacgttct acaattttca cagacggatt taacttagtc gtcaattcta
      181 ctaactcatt actgtccgta ttcaaacaag tcgtgcaatt atgttcagga tgtttttgtt
      241 gatagatatc tatcattgct aaagcgaaat ggactagggg ctttcctgcc aaatatttca
      301 aatttttatt tttaaacccc ttagacccag ctctaccaca aatagtaaat aaaatttcca
      361 tttatttctc ctcagctatt ttcaatactt cgtttgcatg atataaatca ttccagtttt
      421 ctttgcctgt tactacgttt tcaaagaaat atttcaactc cttctcatac atatcattag
      481 cagattcagg catttcttta acaatgtttt cttcaaaact tattttatta tttaataagt
      541 caacattgat acattcatct aaagtaaaca attgaaattt tcttgtagga actcttccaa
      601 aataatctaa atgaatttct ataattttat catcgtatct caatatatat gttgctaaat
      661 cattactgtt aatttcaaga ttcgaatatt taccaaactg tttttttact gatttaggga
      721 aaccaaatag gtttataata taatccaatt catgtataca gtctaattct actcctcccc
      781 ccatagattt aaaggcagaa taattatttc tataatcagt accttttctc caatcaggaa
      841 gataagtaga acaaatagct cttacagaat atactttttt actttgaata ctattacgaa
      901 caacttccat gatattttta tacctcagtg gagcagcaac ataaatattt ttgtaatcat
      961 aattatattg agtaagatca atactctcag aaaaagcagg tttttctaaa aatatgtaat
     1021 caactttttc aacaattaca tttagtgttt ccagatgctt aaaagtcgga tttgtaataa
     1081 aagctatatc ataatgtgta tccaaatcgt ctctattttg aatatacgtt atttcttcaa
     1141 actctttttt tccactgcta ctataagctg ttatatcaaa attaatagaa ttcttttttc
     1201 catagcttat tagatttttt agatgtctct ttccaattga ccctaaccca aaaaaaatta
     1261 ctttcatcat taatttacct caatgtcgtt aattaagtta ataatatact tatctttttc
     1321 aaaagtatat tggggttcca ttttcttttc caaaagcgtg aaaaattctt taatctcc
//
LOCUS       DAYYBH010000179         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000179 DAYYBH010000000
VERSION     DAYYBH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.188"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHAUG_002949"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHAUG_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM8481965.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAYYBH010000180         1362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000180 DAYYBH010000000
VERSION     DAYYBH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.189"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACHAUG_002950"
     CDS             82..>1362
                     /locus_tag="ACHAUG_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8481966.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAYYBH010000181         1359 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000181 DAYYBH010000000
VERSION     DAYYBH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.190"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..208)
                     /locus_tag="ACHAUG_002951"
     CDS             complement(<1..208)
                     /locus_tag="ACHAUG_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481967.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
     gene            complement(305..757)
                     /locus_tag="ACHAUG_002952"
     CDS             complement(305..757)
                     /locus_tag="ACHAUG_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481968.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(764..1312)
                     /locus_tag="ACHAUG_002953"
     CDS             complement(764..1312)
                     /locus_tag="ACHAUG_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8481969.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          487 a          264 c          132 g          476 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
      301 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
      361 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
      421 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
      481 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
      541 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
      601 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
      661 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
      721 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
      781 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
      841 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
      901 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
      961 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
     1021 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
     1081 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
     1141 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
     1201 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
     1261 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
     1321 ctccataaaa aataagttac tttttaaata tcttgttta
//
LOCUS       DAYYBH010000182         1350 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000182 DAYYBH010000000
VERSION     DAYYBH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.191"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..314
                     /locus_tag="ACHAUG_002954"
     CDS             <1..314
                     /locus_tag="ACHAUG_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8481970.1"
                     /translation="NPYFNMVKRIENEVTIVNKSRYTSRQQTPEVFEMNASMYSFKPE
                     FLKKSNYIFDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYR
                     R"
     gene            319..1266
                     /locus_tag="ACHAUG_002955"
     CDS             319..1266
                     /locus_tag="ACHAUG_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8481971.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          451 a          192 c          275 g          432 t
ORIGIN      
        1 gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttactata gttaataaat
       61 caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc tcaatgtatt
      121 cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa tgtgggataa
      181 taaaaatgag agattatcta attttagata ttgatagtga agaagatttt aaatggttag
      241 aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt
      301 tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt
      361 tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca
      421 atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc
      481 gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat
      541 ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg
      601 aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa
      661 cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa
      721 gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa
      781 tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat
      841 gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta
      901 gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga
      961 agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg
     1021 aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt
     1081 ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa
     1141 gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt
     1201 ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc
     1261 aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc aataaggact
     1321 gatgagtttt gtaaaatcaa atctgggata
//
LOCUS       DAYYBH010000183         1324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000183 DAYYBH010000000
VERSION     DAYYBH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.192"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHAUG_002956"
     CDS             278..820
                     /locus_tag="ACHAUG_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481972.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACHAUG_002957"
     CDS             976..>1324
                     /locus_tag="ACHAUG_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8481973.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAYYBH010000184         1294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000184 DAYYBH010000000
VERSION     DAYYBH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.193"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACHAUG_002958"
     CDS             <1..1244
                     /locus_tag="ACHAUG_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8481974.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAYYBH010000185         1289 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000185 DAYYBH010000000
VERSION     DAYYBH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.194"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..710)
                     /locus_tag="ACHAUG_002959"
     CDS             complement(42..710)
                     /locus_tag="ACHAUG_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8481975.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            844..1131
                     /locus_tag="ACHAUG_002960"
     CDS             844..1131
                     /locus_tag="ACHAUG_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481976.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          481 a          215 c          216 g          377 t
ORIGIN      
        1 tgatatttct gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca
       61 agaggatcaa gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca
      121 gatccaattt ccagatactt cactttattc aatgtataag atagaataga acagctaagt
      181 aatgtataca gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa
      241 gtaatcaaga aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa
      301 atgatatctg ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca
      361 gaaagaatac ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga
      421 aggctctccc ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga
      481 aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt
      541 gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc
      601 ccaccatctg ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct
      661 gcgcctaaaa tgattccgca caattctgta aataaattta attttttcat ttctagcctc
      721 ttttcttttg aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag
      781 aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga
      841 aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat
      901 caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat
      961 aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac
     1021 atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa
     1081 ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata
     1141 agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg
     1201 tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt
     1261 tctactttat tggctgtttc tttttatta
//
LOCUS       DAYYBH010000186         1275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000186 DAYYBH010000000
VERSION     DAYYBH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.195"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHAUG_002961"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHAUG_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHAUG_002962"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHAUG_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          451 a          214 c          174 g          436 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaat
//
LOCUS       DAYYBH010000187         1259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000187 DAYYBH010000000
VERSION     DAYYBH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.196"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..1087
                     /locus_tag="ACHAUG_002963"
     CDS             230..1087
                     /locus_tag="ACHAUG_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8481977.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          465 a          183 c          253 g          358 t
ORIGIN      
        1 gtctacatga ctaaagtcgc gagtattctc tgcttctcga taaattaaat aacgtcatta
       61 ctttgaggaa agctataaat caatatattt cagcttttcc ttatttatgt actacctttt
      121 acttattaat tataaggtag tacatatata tctttttttg gtagtaacta aaaataaata
      181 tgttataata gtatcaaatg aatattgcta aaagaaaaga ggtaggaacg tggtagcgaa
      241 gaaatatgta atcggtaatt ttaaaggtgg cgtaggtaaa agtacgtgcg cgcaaatgtt
      301 agggtttgaa tcagcagtta atgaaggaaa aagaaccttg ataattgact tggatatgca
      361 aggaaatact tctgatgtga tgaacttaac gcatatgaac ttcactaacg aagaaggagg
      421 cggagaaggt gaaccgattg tttacgaaaa tactgtgact gacgtgttga taaacaatct
      481 tccacctaaa gaggctgtct ataaagtaat caataacctc tatatacttc cagcagacat
      541 gtcctttgaa atgtatgatg attggatcaa agaccaatat aatgattcat tatcccaatt
      601 caaatatatg aaagaaaagc tagaaccatt atttgatgag tttgacgtca tctatttaga
      661 tgtcccacct tctatttctg tatatagtaa atccgcgatg tacattgcgg attgggcaat
      721 cgtcgtctta cagacccaag taaaatcgat gagaaatgcg atgcaatatc ttgagtatat
      781 ggactttttt gtgaaagaat ttgatacaaa tttatatgtt gctggtgtta ttcctttcat
      841 gttggaaagc ggtgacgcag tagatcagga aatgtataaa caagcccaag agatttatgg
      901 ggaacatttg attaaaaatg ttgtactcaa aaatgcgcgc ttaaaacgat acgacggatc
      961 aggtattacg atggagaaga caaagaaagg tctcttaaaa caatgggatc gcaaaagcca
     1021 cgaattattt atcagtattt tgaaagaatt ggaagaacac gagaattggt atgcggaggt
     1081 ggagtaattg gctgacatca aaggacttga tatttttggg aacaacaaag gactcgccaa
     1141 aataaaaaaa gaagcagaag caaagaaaaa gcagcaagca attacaccat ctgaaaataa
     1201 gcaattagaa gtgcaccaag ttcaagaaat aaaaacttcc tccaatgttg aaagacaga
//
LOCUS       DAYYBH010000188         1251 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000188 DAYYBH010000000
VERSION     DAYYBH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.197"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            285..1100
                     /locus_tag="ACHAUG_002964"
     CDS             285..1100
                     /locus_tag="ACHAUG_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8481978.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
BASE COUNT          481 a          172 c          202 g          396 t
ORIGIN      
        1 ataagaaatt caaaaaagaa aaaactttcc ttaaaaacaa aaaataggag tattcagttt
       61 cttaatagtt ttgtaatagt tgtatgtaga agttttctta atagtttttg taataattta
      121 ttaatcgtag tttatataac ttatttgtaa gtgactagag aattaaataa aaaataatta
      181 ctatctagta cgaaattaat tacataggat aatatattaa ggtgctggac tacaagtaaa
      241 aaatactgta tgatcatgac agattcaaga aaggagcaat aagattggtt aagatagcag
      301 gactttctaa aggtggaaaa accgtttcac aactttataa acaaaactac agagactgga
      361 gacagttcag aaaaaacatt aaggattctt actttattct accaacagaa cttgaaaaat
      421 atttaccgaa taacaaagct attaatttat atctgtatta ctgttttaag gcaaagaatg
      481 aaggcgggga ttcctggcat tcggtcaata ccatagcaga gtctctagga gtgacaacta
      541 aaactataaa cacctggaat aaaaccttag ttgatttagg tatgattgca cgtatagacg
      601 ataaccatct cagtaaatcg acaattttac ttcctctctc tagttactat aaattagccc
      661 ttgatagttc tttaaaggat gttaccgaga gcactgtcca tgaaattgat ggcaacttgg
      721 ttgcagttta tcatttgttt gaatggagaa aaaatgaggc tgatgaaaat tataacgttc
      781 cttataacac actctgttgt gtttttaaaa gaaaaacaca aaaagatact atctataaat
      841 ttatcttggt ccaagatgag aaaataagta atttcgaatt aagcactcct tctagtaaga
      901 tttatgacga tgcatataga tttgaatgtc aagatctaga agccctcctt tccgcaaata
      961 atctaaacaa tatagaacat gaaaactttg ttgtaaacac tagatttaat ttagtttctg
     1021 aaaaggactc agatttgtta gatttgctga tagagcttac taaaaatcta gataagaaag
     1081 aaaacattac tgtactataa ctagcatcgg tccatcatta agtttggact attagaacac
     1141 ttgctattac agaaaaaggt atgctagcca cgcttactat ttattggtat caaacaagta
     1201 tcttaatttg aaaggaagat gatttatggc taatttatat atagagaaca a
//
LOCUS       DAYYBH010000189         1235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000189 DAYYBH010000000
VERSION     DAYYBH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.198"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            281..973
                     /locus_tag="ACHAUG_002965"
     CDS             281..973
                     /locus_tag="ACHAUG_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8481979.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          527 a          167 c          200 g          341 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactaagt agttcagttt tggagtacaa aatgacaaaa tgtacttcaa aatggactac
     1081 ttttttcatt ttaacttaca taccaaatta catacaaaat gatttataaa atgcaattga
     1141 aaatgataat tgaatgaaaa tttgtgattt tgtggtatta tgataatata acttatagaa
     1201 aaaattgctt attcaactaa cgggcaggat aatga
//
LOCUS       DAYYBH010000190         1208 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000190 DAYYBH010000000
VERSION     DAYYBH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.199"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACHAUG_002966"
     CDS             <1..459
                     /locus_tag="ACHAUG_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481980.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACHAUG_002967"
     CDS             473..904
                     /locus_tag="ACHAUG_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481981.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="ACHAUG_002968"
     CDS             906..1043
                     /locus_tag="ACHAUG_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8481982.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
BASE COUNT          442 a          175 c          254 g          337 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga agaagcagat aaaatcatta
     1021 gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag gagttgtcac
     1081 atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat cgtttctttg
     1141 attttattag taataaaacc aattactgca tctttctcga agattactga gactctttca
     1201 aaagtaag
//
LOCUS       DAYYBH010000191         1184 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000191 DAYYBH010000000
VERSION     DAYYBH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.200"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..>1184
                     /locus_tag="ACHAUG_002969"
     CDS             120..>1184
                     /locus_tag="ACHAUG_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481983.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVS"
BASE COUNT          397 a          153 c          153 g          481 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt taaaaaaaat aaatgatact atatgtaata taaaaaaaca
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      481 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      541 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      601 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      661 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      721 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      781 ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta
      841 tgattgagca gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa
      901 gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat
      961 ttggtattgg cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat
     1021 taggaatctt atctaaaatc acttatataa tgggtataat atctattcta ctttgtacaa
     1081 aaatatttcc atggtttgcc ttacaaaata ctcctttaaa tattattcaa gctccttggc
     1141 gttttatgat attttcaaca ttgttcatat ctttttcagt atct
//
LOCUS       DAYYBH010000192         1073 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000192 DAYYBH010000000
VERSION     DAYYBH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.201"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACHAUG_002970"
     rRNA            <1..>1073
                     /locus_tag="ACHAUG_002970"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAYYBH010000193         1068 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000193 DAYYBH010000000
VERSION     DAYYBH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.202"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHAUG_002971"
     CDS             <1..969
                     /locus_tag="ACHAUG_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8481984.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          186 c          213 g          301 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cattacataa ctatattgac aactataaaa
     1021 atcattcgat aaaatagtga tgttatcgca tatcttcact atcaccca
//
LOCUS       DAYYBH010000194         1067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000194 DAYYBH010000000
VERSION     DAYYBH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.203"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACHAUG_002972"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACHAUG_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8481985.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DAYYBH010000195         1062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000195 DAYYBH010000000
VERSION     DAYYBH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.204"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1062)
                     /locus_tag="ACHAUG_002973"
     CDS             complement(37..>1062)
                     /locus_tag="ACHAUG_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8481986.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAYYBH010000196         1040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000196 DAYYBH010000000
VERSION     DAYYBH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.206"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1040
                     /locus_tag="ACHAUG_002974"
     CDS             <1..>1040
                     /locus_tag="ACHAUG_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8481987.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQ"
BASE COUNT          363 a          204 c          187 g          286 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa
//
LOCUS       DAYYBH010000197         1002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000197 DAYYBH010000000
VERSION     DAYYBH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.207"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1002
                     /locus_tag="ACHAUG_002975"
                     /pseudo
     CDS             370..1002
                     /locus_tag="ACHAUG_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cc
//
LOCUS       DAYYBH010000198          991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000198 DAYYBH010000000
VERSION     DAYYBH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.208"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACHAUG_002976"
     tRNA            complement(21..94)
                     /locus_tag="ACHAUG_002976"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ACHAUG_002977"
     tRNA            complement(108..181)
                     /locus_tag="ACHAUG_002977"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ACHAUG_002978"
     tRNA            complement(209..281)
                     /locus_tag="ACHAUG_002978"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ACHAUG_002979"
     tRNA            complement(301..374)
                     /locus_tag="ACHAUG_002979"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="ACHAUG_002980"
     tRNA            complement(384..457)
                     /locus_tag="ACHAUG_002980"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="ACHAUG_002981"
     tRNA            complement(468..553)
                     /locus_tag="ACHAUG_002981"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="ACHAUG_002982"
     tRNA            complement(570..641)
                     /locus_tag="ACHAUG_002982"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="ACHAUG_002983"
     tRNA            complement(653..725)
                     /locus_tag="ACHAUG_002983"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="ACHAUG_002984"
     tRNA            complement(743..824)
                     /locus_tag="ACHAUG_002984"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="ACHAUG_002985"
     tRNA            complement(832..904)
                     /locus_tag="ACHAUG_002985"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="ACHAUG_002986"
     tRNA            complement(908..980)
                     /locus_tag="ACHAUG_002986"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DAYYBH010000199          988 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000199 DAYYBH010000000
VERSION     DAYYBH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.209"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..988
                     /locus_tag="ACHAUG_002987"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACHAUG_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          351 a          175 c          197 g          265 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaa
//
LOCUS       DAYYBH010000200          910 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000200 DAYYBH010000000
VERSION     DAYYBH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.210"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACHAUG_002988"
     CDS             <1..355
                     /locus_tag="ACHAUG_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481988.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACHAUG_002989"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACHAUG_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DAYYBH010000201          898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000201 DAYYBH010000000
VERSION     DAYYBH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.211"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..879
                     /locus_tag="ACHAUG_002990"
     CDS             256..879
                     /locus_tag="ACHAUG_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8481989.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          332 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaaga
//
LOCUS       DAYYBH010000202          884 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000202 DAYYBH010000000
VERSION     DAYYBH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.212"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHAUG_002991"
     CDS             136..309
                     /locus_tag="ACHAUG_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481990.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          295 a          144 c          107 g          338 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaa
//
LOCUS       DAYYBH010000203          839 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000203 DAYYBH010000000
VERSION     DAYYBH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.213"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..528)
                     /locus_tag="ACHAUG_002992"
     CDS             complement(16..528)
                     /locus_tag="ACHAUG_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481991.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            complement(535..>839)
                     /locus_tag="ACHAUG_002993"
     CDS             complement(535..>839)
                     /locus_tag="ACHAUG_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM8481992.1"
                     /translation="FSKNKLKDINSIDNYYIQFKGFDFNSSTEFKLALYAQFFIEKLN
                     RFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
BASE COUNT          291 a          117 c           77 g          354 t
ORIGIN      
        1 ttaaaactaa catccttaat atcttaattt atccatattt tgagaaataa tacttttcca
       61 ttcagaaact aatttagtaa ggaatcttgc aatgttttgt ctcattaatt cattatttct
      121 attatctata agatctgtta gatatttttc aaattctaaa aagctatccg aattttcttc
      181 gacgattaaa tacttaagag aatttatatc ggaaaagata gattcaagtt tactaatctc
      241 attttctaaa tctatatttt cacgtgtgat tatctcatca ctttttctta aaaagctaaa
      301 aattgcatca gttgctttac acttttccat taaagaagta tattcttcaa tttttcttct
      361 ttctttatgg tcatttttta aatcagtttc aatgtctttt ttcatttgtt ctatactgag
      421 tttgttcttt ttgttctttt gagtaaatct aaaattcaaa cacgaaaaaa gtatgttagc
      481 aataacacct ataatagcaa ataaatcatt tatactattt aatttcattt atcatcacaa
      541 acttagaact ttacttatca tctcataaga caagtttcca aattgataat tattggtaga
      601 ttttaaatca ttataatttt cgagaaattt tatataagta cctctcatcg gtttatggct
      661 attaaaagtg ttaaaatcac tatcttcaac tatttcttga aacctattta atttttcaat
      721 aaaaaattgt gcataaagtg ctaacttaaa ttcggtagag ctattaaagt caaaaccttt
      781 aaattgtata taataattat caattgaatt tatatctttt aatttatttt tactaaaag
//
LOCUS       DAYYBH010000204          822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000204 DAYYBH010000000
VERSION     DAYYBH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.214"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACHAUG_002994"
     CDS             complement(<1..153)
                     /locus_tag="ACHAUG_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8481993.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
     gene            231..809
                     /locus_tag="ACHAUG_002995"
                     /pseudo
     CDS             231..809
                     /locus_tag="ACHAUG_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT          259 a          151 c          158 g          254 t
ORIGIN      
        1 actcgtttta gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg
       61 aatgatttct acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc
      121 ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc
      181 tagtctacac accttaattt ttctgtctag ttaattgtag cctatccaca ggaatcaaat
      241 cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc ggttatcttt
      301 ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt tttacggatg
      361 ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca gtaattgatc
      421 caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac gcccacgttg
      481 atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac attgctaatt
      541 ataaatacga gtatattttc attatggact acaatactgg aaaagaacta gctagagtaa
      601 gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta ggaaatgttg
      661 gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac gtcatgatgc
      721 gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt catgacaagc
      781 gttggtattt aaatattcca caacgataaa aaattccccc ct
//
LOCUS       DAYYBH010000205          818 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000205 DAYYBH010000000
VERSION     DAYYBH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.215"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACHAUG_002996"
     tRNA            8..81
                     /locus_tag="ACHAUG_002996"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..210
                     /locus_tag="ACHAUG_002997"
     tRNA            121..210
                     /locus_tag="ACHAUG_002997"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            225..296
                     /locus_tag="ACHAUG_002998"
     tRNA            225..296
                     /locus_tag="ACHAUG_002998"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Glu,seq:ttc)
     gene            312..384
                     /locus_tag="ACHAUG_002999"
     tRNA            312..384
                     /locus_tag="ACHAUG_002999"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Val,seq:tac)
     gene            394..466
                     /locus_tag="ACHAUG_003000"
     tRNA            394..466
                     /locus_tag="ACHAUG_003000"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:427..429,aa:Asp,seq:gtc)
     gene            502..577
                     /locus_tag="ACHAUG_003001"
     tRNA            502..577
                     /locus_tag="ACHAUG_003001"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Phe,seq:gaa)
     gene            579..659
                     /locus_tag="ACHAUG_003002"
     tRNA            579..659
                     /locus_tag="ACHAUG_003002"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Tyr,seq:gta)
     gene            666..739
                     /locus_tag="ACHAUG_003003"
     tRNA            666..739
                     /locus_tag="ACHAUG_003003"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Trp,seq:cca)
     gene            742..814
                     /locus_tag="ACHAUG_003004"
     tRNA            742..814
                     /locus_tag="ACHAUG_003004"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:775..777,aa:His,seq:gtg)
BASE COUNT          178 a          188 c          224 g          228 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgccaaact
      181 gtctcaaggg ttcgaatccc ttactctccg tagcaaattt acctggcccg ttggtcaagc
      241 ggttaagaca ccgccctttc acggcggtaa cacgggttcg aatcccgtac gggtcatttt
      301 aattgaatgt tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
      361 gcggttcgat cccgttaacc tccataatga actggttccg tggtgtaggg gttaacatgc
      421 ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg gaaccgtcgt tcttgttatt
      481 caattaataa ttttaaaata tggcgcggta gctcagttgg tagagcaacg gattgaagct
      541 ccgtgtgtcg gcagttcgat tctgtcccgc gccaccatgg aggagtagcg aagtggctaa
      601 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
      661 tttatagggg catagtttaa aggtagaaca gcggtctcca aaaccgttag tgtgggttca
      721 attcctgctg cccctgccat ggcgaatgtg gcgaagtggt taacgcaccg gattgtggct
      781 ccggcattcg tgggttcgat tcccatcatt cgcccttt
//
LOCUS       DAYYBH010000206          773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000206 DAYYBH010000000
VERSION     DAYYBH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.216"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACHAUG_003005"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACHAUG_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8481994.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="ACHAUG_003006"
     CDS             complement(481..675)
                     /locus_tag="ACHAUG_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481995.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          229 a          162 c          134 g          248 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaag acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttacgtt aaaaatgttt tgc
//
LOCUS       DAYYBH010000207          752 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000207 DAYYBH010000000
VERSION     DAYYBH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.217"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..>752)
                     /locus_tag="ACHAUG_003007"
     CDS             complement(91..>752)
                     /locus_tag="ACHAUG_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFM8481996.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          204 a          176 c          144 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gc
//
LOCUS       DAYYBH010000208          725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000208 DAYYBH010000000
VERSION     DAYYBH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.218"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACHAUG_003008"
     CDS             <1..>725
                     /locus_tag="ACHAUG_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8481997.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAYYBH010000209          707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000209 DAYYBH010000000
VERSION     DAYYBH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.219"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..598
                     /locus_tag="ACHAUG_003009"
     CDS             <1..598
                     /locus_tag="ACHAUG_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8481998.1"
                     /translation="MKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVG
                     GGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKK
                     GQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLV
                     NGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          199 a          139 c          180 g          189 t
ORIGIN      
        1 catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg cggctgccta
       61 tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact atacgatcgg
      121 tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt ttagtcatcc
      181 agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg gcagctgtcc
      241 atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa aacgcttagg
      301 gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag cctcctatat
      361 tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca tttttggcgg
      421 tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg caaaattggt
      481 caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag cattagaaga
      541 caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac attcataaac
      601 gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa aatttagtca
      661 taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DAYYBH010000210          695 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000210 DAYYBH010000000
VERSION     DAYYBH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.220"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            225..482
                     /locus_tag="ACHAUG_003010"
     CDS             225..482
                     /locus_tag="ACHAUG_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8481999.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          284 a           93 c           90 g          228 t
ORIGIN      
        1 tagtttcgtg cgcttcaaaa catattactc aataaagaaa atcttaaatt tcatcttgac
       61 ttatcaccat tagactaaat atttttctgt ccacgttcat attactccaa agttttcctc
      121 attccaaaag aaaagcacag aatacacaaa gaagcgtttt tatttgtata aactatatca
      181 aagttctata cttaaattat caaataaaaa ggacgtgaag ataaatgtca aaattagaaa
      241 acaaagaaga aaaagttgta aacaaaattg tttctgttgt caataaactg gataaagagt
      301 tagacgaact tgatacgtta tctgaaaatc cagaaaaaaa acataaccta aaaaaatggt
      361 tagttgaacg aaaggctatt catgaaatta aaaaagttct tcatgaagct gataaatatg
      421 aaaaatatga tgaaaaagaa cttgacaaag aatttaaaga aatcaatgac ttattactat
      481 aattgattta tacgactcat ttttgagtcg tcagactgca aacaacactc taaatttagg
      541 agtttgtcta cagtctgaat aaattttaaa tttattgtcc ataatgttat tatgaattta
      601 tagcattaag aaaagagata agactttaca tttttttagg aatgtaaagt cttatctctt
      661 ttcttaattg tacaattagt attatctgca ctact
//
LOCUS       DAYYBH010000211          693 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000211 DAYYBH010000000
VERSION     DAYYBH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.221"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..431)
                     /locus_tag="ACHAUG_003011"
     CDS             complement(<1..431)
                     /locus_tag="ACHAUG_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8482000.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPK"
BASE COUNT          247 a          120 c           65 g          261 t
ORIGIN      
        1 attttgggtt tctttatatt ttcatattta atagacatta ccatataaac atatggaata
       61 aaaagaataa tattttctaa acacgctgta gataactctt ttgttccagt tgttgtatta
      121 tacttataaa tccaccagtc tcccataaca tcagataaag gatttaacca aattgttcga
      181 ttgattaatg ttgtaaataa aatcatcgca gtatagaata tcaaagcaca aacttttcta
      241 tattttctat ccttctttaa atgaagataa agttccatta gtatacgttt atagccctca
      301 cctgcagcaa tgccatctgt tgcatacata aaatagacca gtcctaaaat tgtcaataaa
      361 acagcaaacc caaagggttc atataattta tataatatat ctgtaataat ttctgcaatc
      421 actattatca taattatttc tccaatatca agtatacact tatcgcttcc aattacacat
      481 gatcatatta gagtgtatta tcacattctc taatattcaa tttatttttc ctaaaatatt
      541 cgaaataatc gtcttgataa cgagtaagta tttttatacg attgataaca tagtaacaat
      601 atttttaatt atagtaaaaa atcatctgcc tttttcatcc acctaatctt atttttcatt
      661 ttattcaaca tccttaaata aacttccgtt cct
//
LOCUS       DAYYBH010000212          617 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000212 DAYYBH010000000
VERSION     DAYYBH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.223"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          215 a           76 c          141 g          185 t
ORIGIN      
        1 attatagaaa aaatttataa aaggaggttt cttgatatga taagaaacaa aaagggtttg
       61 ttgaaagttg ctttactggc agcttttttc taagtaactt tttgacaagc tcaatggcaa
      121 cacaagcgga agaagtaata caagaactaa ctgttatgaa taacagctag ataaaatggg
      181 ctatgtatgt tatccaacaa tttagtaatc actagaagcc tagcgaaagt tcgcttgata
      241 agttatagat gagtattgat ttgtagtgtt tccaaatcaa aagggtacag agccacatga
      301 aatatgttgt cctctgcagc gtcaggatgg gaatccatac catggagatt cggagatctg
      361 tgagggaata cttacgtggt taatatgtca gttgtgttgg acgtagtaat ggtcatagtg
      421 ggtagattga ctgaattctt atatgagaaa atgaaaccaa caagaagata gggttattta
      481 tacagaattg gggatctttt ctggaaaagt aatcggataa tgcgagattc gtacggtctt
      541 ttttaaattt ctctaaaaaa aacggcatcg aatgggttgg gtgattgata agcaatgaat
      601 catagactga tatagaa
//
LOCUS       DAYYBH010000213          514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000213 DAYYBH010000000
VERSION     DAYYBH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.224"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHAUG_003012"
     CDS             complement(<1..132)
                     /locus_tag="ACHAUG_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8482001.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAYYBH010000214          508 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000214 DAYYBH010000000
VERSION     DAYYBH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.225"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DAYYBH010000215          506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000215 DAYYBH010000000
VERSION     DAYYBH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.226"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          234 a           67 c           40 g          165 t
ORIGIN      
        1 tataattaaa tactaccaga aaaacgtatg aataaaattt agttttaaaa ttaaagataa
       61 ttaagtacta ccagtgtcaa aacaaaaaat aaattaagaa ctaccttatg catataaaac
      121 atactaccat tactagtaat atatactacc ttatttcagt agtatatatt atattattct
      181 tataagacaa ggctttaggc tagaaaacaa tagattatat actactaaaa aatacaataa
      241 gtactaccaa tgtcagatat atataataat tatgtactac cattaaaatt aacttattta
      301 ataagatact actgtgttaa tctgaaaaaa attactttaa agtagttaca tactacctgt
      361 taatttagat actaccatta atacttaaat tagatactac ctatatttat attaagtact
      421 accgttctaa gaataagata ataaaaaaat actaccaaat tgcaattaaa tactacttaa
      481 aatgtaaaaa ataagaacta cccaaa
//
LOCUS       DAYYBH010000216          495 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000216 DAYYBH010000000
VERSION     DAYYBH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.227"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..165
                     /locus_tag="ACHAUG_003013"
     CDS             43..165
                     /locus_tag="ACHAUG_003013"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8482002.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
BASE COUNT          185 a           73 c           87 g          150 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catcgccgat tgtaaaatta
      361 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      421 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      481 gtcttatttc aaaat
//
LOCUS       DAYYBH010000217          494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000217 DAYYBH010000000
VERSION     DAYYBH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.228"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..326
                     /locus_tag="ACHAUG_003014"
     CDS             <1..326
                     /locus_tag="ACHAUG_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8482003.1"
                     /translation="KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQR
                     EGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
BASE COUNT          198 a           66 c           90 g          140 t
ORIGIN      
        1 aaaaggcaag tttaaaatca ctaaaagata catggcttga tttatcagaa gataatccat
       61 acagccaatt cttaattact gtaatggctg gtgttaacca attagagcga gatcttattc
      121 ggatgagaca acgtgaaggg attgaattgg ctaagaaaga aggaaagttt aaaggtcgat
      181 taaagaagta tcataaaaat cacgcaggaa tgaattatgc ggtaaagcta tataaagaag
      241 gaaatatgac tgtaaatcaa atttgtgaaa ttactaatgt atctagggct tcattataca
      301 ggaaattatc agaagtgaat aattagccat tctgtattcc gctaatgggc aatattttta
      361 aagaagaaaa ggaaactata aaatattaac agcctcctag cgatgccgaa aagccctttg
      421 ataaaaaaag aatcatcatc ttaagaaatt cttagtcatt tattatgtaa atgcttataa
      481 attcggccct ataa
//
LOCUS       DAYYBH010000218          489 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000218 DAYYBH010000000
VERSION     DAYYBH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.229"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          181 a           63 c           77 g          168 t
ORIGIN      
        1 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacattc
       61 taatatattc atatgagcta attttatgat taaatggtaa gtttttcctt gtttgtttta
      121 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga tctgtggggt aaatcattat
      181 acaaaagtaa aattttactt attaacaggc tgtagaaata taaaaaagaa ctaaaattta
      241 actaaattat gtgctattct taaggaaagc caagtgtatg agaaagtttc tgagaatata
      301 attttgagag tataaaatat tttgtgtaaa tgaaaaaatc catacaaaaa aggaagtccc
      361 ttctgtagaa taaagttaac gacaaccaat tcacagaaaa gaggacttcc ctatgaatga
      421 ttttactaca gaaattgtgc aaactctagt cactaaaggc gatttaaatg aattattccg
      481 ttcgcactt
//
LOCUS       DAYYBH010000219          476 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000219 DAYYBH010000000
VERSION     DAYYBH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.230"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>476
                     /locus_tag="ACHAUG_003015"
     CDS             22..>476
                     /locus_tag="ACHAUG_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8482004.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DAYYBH010000220          462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000220 DAYYBH010000000
VERSION     DAYYBH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.231"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACHAUG_003016"
     CDS             complement(<1..430)
                     /locus_tag="ACHAUG_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8482005.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAYYBH010000221          460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000221 DAYYBH010000000
VERSION     DAYYBH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.232"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHAUG_003017"
     tRNA            complement(20..91)
                     /locus_tag="ACHAUG_003017"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHAUG_003018"
     tRNA            complement(113..200)
                     /locus_tag="ACHAUG_003018"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHAUG_003019"
     tRNA            complement(225..298)
                     /locus_tag="ACHAUG_003019"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHAUG_003020"
     tRNA            complement(312..382)
                     /locus_tag="ACHAUG_003020"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHAUG_003021"
     tRNA            complement(384..459)
                     /locus_tag="ACHAUG_003021"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAYYBH010000222          449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000222 DAYYBH010000000
VERSION     DAYYBH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.233"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..270)
                     /locus_tag="ACHAUG_003022"
                     /pseudo
     CDS             complement(34..270)
                     /locus_tag="ACHAUG_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           76 c           77 g          150 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaataacgc cgtttgtata attaagcgag acaaataaaa
      301 aagccattta tgttacactc caattgtaat acccgctaaa gcatacaagg agagtgaaca
      361 taaatgactt atacccatct tacaccaaac gagcttgtaa tgatagaagc atattttcat
      421 caagaaactc cggttgctat cgttgcgaa
//
LOCUS       DAYYBH010000223          447 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000223 DAYYBH010000000
VERSION     DAYYBH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.234"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>447
                     /locus_tag="ACHAUG_003023"
     CDS             <1..>447
                     /locus_tag="ACHAUG_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFM8482006.1"
                     /translation="QSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWLRKKRDTQ
                     AAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLNNLIEQD
                     HRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          148 a           91 c           90 g          118 t
ORIGIN      
        1 caatccttct attcatggaa aatggacgaa acctatatca aaattaaggg acgttggcat
       61 tatctttatc gtgcaattga tgcggacggc ttaaccttag atatctggtt acgaaagaaa
      121 cgggatacgc aagcagccta tgctttctta aaacgactcc ataaacagtt tggtgagccg
      181 aaagcaattg tgaccgataa agcaccttct cttggctccg cctttagaaa gttacagagt
      241 gtgggtttat atactaagac agagcaccga actgtgaagt atcttaacaa tttaatagaa
      301 caagaccatc gacctattaa acgacgaaat aaattttatc aaagtctccg tacagcctct
      361 tccacgatta agggcatgga gaccattcga ggaatatata aaaagaaccg aagaaatgga
      421 acgctcttcg gcttttcggt gtctact
//
LOCUS       DAYYBH010000224          443 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000224 DAYYBH010000000
VERSION     DAYYBH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.235"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>443
                     /locus_tag="ACHAUG_003024"
     CDS             82..>443
                     /locus_tag="ACHAUG_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8482007.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIA"
BASE COUNT          155 a           76 c           88 g          124 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgc
//
LOCUS       DAYYBH010000225          421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000225 DAYYBH010000000
VERSION     DAYYBH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.236"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACHAUG_003025"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACHAUG_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAYYBH010000226          411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000226 DAYYBH010000000
VERSION     DAYYBH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.237"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACHAUG_003026"
     rRNA            <1..>411
                     /locus_tag="ACHAUG_003026"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAYYBH010000227          404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000227 DAYYBH010000000
VERSION     DAYYBH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.238"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>404
                     /locus_tag="ACHAUG_003027"
     CDS             <1..>404
                     /locus_tag="ACHAUG_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8482008.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAYYBH010000228          386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000228 DAYYBH010000000
VERSION     DAYYBH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.239"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACHAUG_003028"
     CDS             <1..>386
                     /locus_tag="ACHAUG_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8482009.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAYYBH010000229          386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000229 DAYYBH010000000
VERSION     DAYYBH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.240"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACHAUG_003029"
     CDS             <1..>386
                     /locus_tag="ACHAUG_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8482010.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAYYBH010000230          362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000230 DAYYBH010000000
VERSION     DAYYBH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.241"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACHAUG_003030"
     rRNA            complement(<1..>362)
                     /locus_tag="ACHAUG_003030"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAYYBH010000231          351 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000231 DAYYBH010000000
VERSION     DAYYBH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.242"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHAUG_003031"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHAUG_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8482011.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          113 a           52 c           56 g          130 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa a
//
LOCUS       DAYYBH010000232          344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000232 DAYYBH010000000
VERSION     DAYYBH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.243"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACHAUG_003032"
     CDS             <1..>344
                     /locus_tag="ACHAUG_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8482012.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAYYBH010000233          342 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000233 DAYYBH010000000
VERSION     DAYYBH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.244"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..>342
                     /locus_tag="ACHAUG_003033"
     CDS             26..>342
                     /locus_tag="ACHAUG_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8482013.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLR"
BASE COUNT          122 a           47 c           71 g          102 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aa
//
LOCUS       DAYYBH010000234          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000234 DAYYBH010000000
VERSION     DAYYBH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.245"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACHAUG_003034"
     CDS             <1..>321
                     /locus_tag="ACHAUG_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8482014.1"
                     /translation="VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQV
                     TEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPF
                     VTVK"
BASE COUNT          130 a           39 c           51 g          101 t
ORIGIN      
        1 tggtgaaaga aatgaatttt caaggaactt attctaatga acaaatggaa aataaacttg
       61 aagaatttga gaattttgat tatgtaccta taggctataa aaaagatcaa ttgcttttga
      121 aaaaccatca agttacagaa gatggtcaag ttatttatag tagcttttct ccaaataaag
      181 atgaaattac atttacgttt aaatcttcaa aaggcaaaac aatagtttta cctgtattcc
      241 ggtataaagg attaacggtt actgttaatt caaaaaatgc tacagtacta aaacaaggag
      301 gtccttttgt tacagtaaaa a
//
LOCUS       DAYYBH010000235          315 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000235 DAYYBH010000000
VERSION     DAYYBH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.246"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>315
                     /locus_tag="ACHAUG_003035"
     CDS             <1..>315
                     /locus_tag="ACHAUG_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8482015.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTT"
BASE COUNT          109 a           62 c           72 g           72 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgac
//
LOCUS       DAYYBH010000236          313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000236 DAYYBH010000000
VERSION     DAYYBH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.247"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>313
                     /locus_tag="ACHAUG_003036"
     CDS             28..>313
                     /locus_tag="ACHAUG_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8482016.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT           89 a           61 c           63 g          100 t
ORIGIN      
        1 tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa tggtagaaat
       61 caatttgcga tgcttacgat tgatgatcta gttcctcaag atcatctcgt tcgaaagatt
      121 gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta ctccgattta
      181 ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca gtatttattc
      241 ggtatccgtt ccatgcgaca aaccattaag gaagtcgata ccaatgtcgc ttaccgctgg
      301 tttttaggct act
//
LOCUS       DAYYBH010000237          299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000237 DAYYBH010000000
VERSION     DAYYBH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.248"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACHAUG_003037"
     CDS             <1..>299
                     /locus_tag="ACHAUG_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8482017.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAYYBH010000238          299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000238 DAYYBH010000000
VERSION     DAYYBH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.denovo.249"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..299
                     /locus_tag="ACHAUG_003038"
                     /pseudo
     CDS             1..299
                     /locus_tag="ACHAUG_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           59 c           56 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcag
//
LOCUS       DAYYBH010000239        22597 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.guided.044, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000239 DAYYBH010000000
VERSION     DAYYBH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.guided.044"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACHAUG_003039"
     CDS             complement(134..577)
                     /locus_tag="ACHAUG_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8482018.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACHAUG_003040"
     CDS             complement(795..2135)
                     /locus_tag="ACHAUG_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8482019.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACHAUG_003041"
     CDS             2404..3300
                     /locus_tag="ACHAUG_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8482020.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACHAUG_003042"
     CDS             complement(3450..4391)
                     /locus_tag="ACHAUG_003042"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8482021.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACHAUG_003043"
     CDS             4517..5638
                     /locus_tag="ACHAUG_003043"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8482022.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHAUG_003044"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHAUG_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8482023.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACHAUG_003045"
     CDS             7465..7992
                     /locus_tag="ACHAUG_003045"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8482024.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACHAUG_003046"
     CDS             8203..9264
                     /locus_tag="ACHAUG_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8482025.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACHAUG_003047"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACHAUG_003047"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8482026.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACHAUG_003048"
     CDS             10177..12159
                     /locus_tag="ACHAUG_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8482027.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACHAUG_003049"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACHAUG_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8482028.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="ACHAUG_003050"
     CDS             12881..14305
                     /locus_tag="ACHAUG_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8482029.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="ACHAUG_003051"
     CDS             complement(14486..15259)
                     /locus_tag="ACHAUG_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8482030.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="ACHAUG_003052"
     CDS             complement(15256..16161)
                     /locus_tag="ACHAUG_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8482031.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="ACHAUG_003053"
     CDS             complement(16315..16635)
                     /locus_tag="ACHAUG_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8482032.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="ACHAUG_003054"
     CDS             17109..17795
                     /locus_tag="ACHAUG_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8482033.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="ACHAUG_003055"
     CDS             17797..18741
                     /locus_tag="ACHAUG_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8482034.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="ACHAUG_003056"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="ACHAUG_003056"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8482035.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="ACHAUG_003057"
     CDS             19663..20940
                     /locus_tag="ACHAUG_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8482036.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            21198..22415
                     /locus_tag="ACHAUG_003058"
     CDS             21198..22415
                     /locus_tag="ACHAUG_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8482037.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
BASE COUNT         7297 a         4131 c         4666 g         6503 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaacatact aagattagag gggaacatca ttgacgaatg atgttctcct ctattttcta
    21001 tttttagatt atagtgaaga gtgaaagaaa gttcgaacaa agccttgagg actacgagtc
    21061 cctatcaaaa accgaacttc tttactgtca ttttagaatc aggtttacgt atgttggggc
    21121 catgagcccg tgtataggaa attggaatcg aaatgaagca gtaaagttct ttcaaaactt
    21181 aaaaaggagg aaaacaaatg ccttacattc ttgcattaga tgtttcaatg ggacacagct
    21241 acagtgtcct atacaaagac gacacctgct tatttgaaga cgagattgaa cacactcaaa
    21301 gaggatttca tgcattactc gaagagatcc agcacttgcc tgagcgtcca ttgattgttt
    21361 ttgaagcaac ggggatttat tccagaccag tcgaaaagtt ctttcaagat catcactttt
    21421 cttactgttt gttaaacccg ttagaggcga aaaaacagat ggaagaaagc accttacgca
    21481 gttggaaaac agataaatcg gatgcccacc ggctcgcaca gactcatctt aaaaatcaac
    21541 gtcagccaaa agaagtacaa agcaattttt acttagagat gcgcgattta gcccgttttt
    21601 atcaagaaat tgagaagaaa atcactcgtc tgcgcatgga tttacacaac tgccttcaat
    21661 tgacttttcc agagcttgaa cagttctttt caaacaggct gaccccttac gcattgacat
    21721 tgatccgtct ttttccccat ccagattttg tattggcatc tactcggaca aaaataaaaa
    21781 ataaactgat caacgaaaca cggaaaaaaa tctccgcgaa tcgtgccgag caaaaggcag
    21841 atcaaatcat ccactatgca cagtgtgcct atccagctgt tgaaaaagac agtatccact
    21901 gtcaaaaaac gatctattac gccgaacttc tccaagattt actcgaacaa aaaaaagcac
    21961 tggcgacaca aatgatcaaa aaagccgaag gctctccttg ctttttgttg taccaaacgt
    22021 tccctgggat tggcgcactc acagccgctt tattgctcgg tgaattagga gatatcacac
    22081 gatttaaaac ccataaacaa ttgaatgcct ttgtcggaat cgacatccga cggtatcatt
    22141 caggtaagta tactggccaa gaccgcatca ataagcgtgg taatcccaaa gcacggaaaa
    22201 ttattttctt caccattcga aatatgattc gccagcaacg cgcagctcct aatcatattg
    22261 tcgattatta ctacaaatta aaaaagcaac ctatccctaa gaaagataag gttgccacag
    22321 ttgcttgtat gaacaagctt ctgaaatgta tgcacgctat ggttagggca catacagagt
    22381 acgactatgc gtacgcggtc tcagtggacc attaactaaa ccacttacag tataccactt
    22441 ctttgcccag atcgaaaatt ttttacgaac ggggctttat ttggtgtgct ttgattttgt
    22501 cgctttcgtt tacgagctat ttttcttgca ccttttactt gactaatcgt aggaaataga
    22561 ggctgggaca gaagcgttta actccaagaa ataagaa
//
LOCUS       DAYYBH010000240        16329 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.guided.055, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000240 DAYYBH010000000
VERSION     DAYYBH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.guided.055"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..827
                     /locus_tag="ACHAUG_003059"
     CDS             <1..827
                     /locus_tag="ACHAUG_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8482038.1"
                     /translation="KESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYV
                     ADGSETSSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFS
                     VAPIRALRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASA
                     YIAEIVRGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKD
                     TTIISAIGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            829..1566
                     /locus_tag="ACHAUG_003060"
     CDS             829..1566
                     /locus_tag="ACHAUG_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8482039.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(1737..2930)
                     /locus_tag="ACHAUG_003061"
     CDS             complement(1737..2930)
                     /locus_tag="ACHAUG_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8482040.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(3009..3215)
                     /locus_tag="ACHAUG_003062"
     CDS             complement(3009..3215)
                     /locus_tag="ACHAUG_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8482041.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(3208..4350)
                     /locus_tag="ACHAUG_003063"
     CDS             complement(3208..4350)
                     /locus_tag="ACHAUG_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8482042.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            4862..5497
                     /locus_tag="ACHAUG_003064"
     CDS             4862..5497
                     /locus_tag="ACHAUG_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8482043.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            5651..5728
                     /locus_tag="ACHAUG_003065"
                     /pseudo
     CDS             5651..5728
                     /locus_tag="ACHAUG_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            6042..6089
                     /locus_tag="ACHAUG_003066"
                     /pseudo
     CDS             6042..6089
                     /locus_tag="ACHAUG_003066"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6105..8024
                     /gene="tet(M)"
                     /locus_tag="ACHAUG_003067"
     CDS             6105..8024
                     /gene="tet(M)"
                     /locus_tag="ACHAUG_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFM8482044.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            8143..8310
                     /locus_tag="ACHAUG_003068"
     CDS             8143..8310
                     /locus_tag="ACHAUG_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8482045.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(8370..8723)
                     /locus_tag="ACHAUG_003069"
     CDS             complement(8370..8723)
                     /locus_tag="ACHAUG_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8482046.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            9227..9646
                     /locus_tag="ACHAUG_003070"
     CDS             9227..9646
                     /locus_tag="ACHAUG_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8482047.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            9672..9878
                     /locus_tag="ACHAUG_003071"
     CDS             9672..9878
                     /locus_tag="ACHAUG_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8482048.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(10106..10279)
                     /locus_tag="ACHAUG_003072"
     CDS             complement(10106..10279)
                     /locus_tag="ACHAUG_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8482049.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            10291..10920
                     /locus_tag="ACHAUG_003073"
     CDS             10291..10920
                     /locus_tag="ACHAUG_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8482050.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(11002..12168)
                     /gene="dnaJ"
                     /locus_tag="ACHAUG_003074"
     CDS             complement(11002..12168)
                     /gene="dnaJ"
                     /locus_tag="ACHAUG_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8482051.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(12319..14148)
                     /gene="dnaK"
                     /locus_tag="ACHAUG_003075"
     CDS             complement(12319..14148)
                     /gene="dnaK"
                     /locus_tag="ACHAUG_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8482052.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(14199..14762)
                     /gene="grpE"
                     /locus_tag="ACHAUG_003076"
     CDS             complement(14199..14762)
                     /gene="grpE"
                     /locus_tag="ACHAUG_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8482053.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(14856..15899)
                     /gene="hrcA"
                     /locus_tag="ACHAUG_003077"
     CDS             complement(14856..15899)
                     /gene="hrcA"
                     /locus_tag="ACHAUG_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8482054.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(16133..>16329)
                     /locus_tag="ACHAUG_003078"
     CDS             complement(16133..>16329)
                     /locus_tag="ACHAUG_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HFM8482055.1"
                     /translation="KKKTGITKKHFYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVY
                     LTPRGTFLGNNVFERFLFEK"
BASE COUNT         4760 a         3180 c         3057 g         5332 t
ORIGIN      
        1 aaaaagaatc tggcggaaaa tatgcatttg ccgttaaaaa aggacaaaat cctgagcttc
       61 ttaatatgtt caatgaagga ttgaaagaat taaaaagaac aggcgaatac gataagatta
      121 ttaaagaata tgtagcggat ggttcagaaa cttccagtga gtcttccact gctgatgaat
      181 caacatttct tggacttatc caaaacaact atcaagcatt gttgaaagga ctttggagaa
      241 caattctcct aactttgatc tcctttgctt tggctatagt aatcggtgtg atttttggat
      301 tattcagtgt agcaccaata cgcgctcttc gcacaattgc tagtatttat gtcgatatca
      361 tccgaggaat tccaatgatg gttcttgcgt tctttatttt ctttgggttg cctggtatcg
      421 ttggatttac gattcctgac ttccttgctg gtgttatcac cttgactctt aacgcaagtg
      481 cttatatcgc agagattgtt cgaggcggga tcaacgccgt tcctgtcgga caaatggaag
      541 cttccagaag tttaggtctt tcttataatc ggacaatgca aaaaatcatt ttgccgcaag
      601 cgatacgcat catgatccca tcattcgtca atcagttcgt tatctcatta aaagacacaa
      661 cgatcatttc cgcaatcggt gtagtcgaat tgctgcaaac tggtaagatc attgttgcac
      721 gtacaacaca aagtacgtat gtttacttga tcattgcgat catgtacttg attttgatca
      781 cagcattgac aaaattagca aaagttttag aaaagaaggt gaagtaagat ggcagaaaaa
      841 atcttagttg agcatttagt caaaaaatac ggcgataaca cagtattaaa cgacatcaat
      901 gtatccatca acgaaggaga tgtcgtctgt atcattggcc cttcaggttc aggaaaaagt
      961 acattcttgc gttgcttgaa tcgtttagaa gaaccttctg acggtgaaat tattattgac
     1021 ggcgcccact tgatggataa aagcacagat atcaacttgg tgagacaaca tatcggaatg
     1081 gtattccaac actttaacct attccctcat ctttctattt tggaaaacat cattttagca
     1141 ccgatggacc tgaagaaaga atctagagaa gaagcagaga aaaaagcaat caaattactg
     1201 cagacagttg gtctagaaga taaaaaagat atgtatcctg aaatgctttc tggtggacaa
     1261 aaacagcgtg tggctattgc tcgtgctcta gcgatgaacc ctgacatcat gctttttgat
     1321 gagcctactt ctgcactcga tcctgaaatg gtcggtgatg tcttgaacgt catgaaaaag
     1381 ttagcagaac aaggtatgac gatggttatc gttacgcacg aaatggggtt cgctaaagaa
     1441 gtggccaatc gcgtcatgtt cattgacgac ggaaactttt tagaagatgg aacgcctcaa
     1501 caaatctttg aaaatccgca aaatgaacgg acaaaagatt tcttgaataa agtattaaat
     1561 atttaaaata aattatatat gtctttttga ctatcttttt atatcttctt aaatgtccgc
     1621 aaagccttat tttataggct ctacgggcat tttgcttttg tggtaaacct cacatatcta
     1681 ggtctatctt cttatatttt cactatcaag cgtggttaaa atcgtggtaa atacttctat
     1741 gcaattagac gctgaacctc tgattttgcg gtatctatgg acgcatgagc ataccagttc
     1801 attgtgatac tgatatttga atgccccatg atatactgta aatcttttgg gttcatgttc
     1861 ttgcttgcca gtcttgtgca gaatgtatgg cgtagcgtat gcggtgtgat atgtggcaat
     1921 ggattgtcct tgtggtgctt gttgtatttc tttaccatac ggataaataa ggcgttataa
     1981 tcaatcgcaa ctttgggctt gcccttatga ttgacaaata ggaagttgcc ccgtccgtct
     2041 atcacaaatg gttctgcctt tggacgtttt ttcataacct gttgaaatgc ctgtatcgtt
     2101 tctttgctta atggtacttg ccttattccg ctttttgttt taggcgtttc aatataatag
     2161 ccctgttcct tgctctttag taactggtgg tcgataatca caacttcatt cttgaaatca
     2221 atgtcggcta ctgtcagtcc gcacagttcc gaaatacgaa gtcctgtctt taacagtatc
     2281 agcacatcat cataatgctt gtgatacaca ttgtccgtct tgatgaatga gagtaaggct
     2341 tgttcctgtt cctctgtcaa tgcgactttc tttttggtat cattttctag gacttcactc
     2401 aacttgaaat caaagggatt tttcctcaca taatcatctt gaatggctat gtagaatgac
     2461 gcttttaacg agcgtttatg gttgttaatg gtattgtagg aaaagccttt gtctttcatg
     2521 cgtaacgccc attccttagc gtcagagggt ttgattgtat caatgctcct agcacctaac
     2581 ttgtcctctt tcaataaccg catgagttgt tcccgttgtt tctgtgtgct tttcttcaca
     2641 tttgccctct gtgcgttctg tttggcataa agttggcaaa gcgtcatttt cttgccagtg
     2701 ctgtcgatac cgtcctcaat atcccgtctt atctgctgtt ccagttctcg aagtgagggt
     2761 ttttcccgtt ttccctttgg tgtcgggtct gtgggtgtca atctccaagc atacacatat
     2821 tttgtgtttc caaatgcgtc tacatattta tataagtatt ttctgtctgt tcgttggctc
     2881 tctccagtat gcaggatacg acctttgtta tcccgtcttt tttctttcat ggtgtctgct
     2941 cctttccttg ttggaaagag ccttgatatg acttgtgttc atcataacac atacaaggct
     3001 catttgcatt agatagcgtc caatttatca atgacctgtt caaactgtcg gcgtttaatc
     3061 tgtatgcggt tgccattcat aatgagccag ccagcgtcct tgttttcctc tgccaatcgt
     3121 cttaacttgt tttctccgat acggaaatac tttgacgctt cttcaatggt aagggtgtat
     3181 ttttcccata cgggaatatc ctgtgcatta ctcataaaac accccctcac tttcgattac
     3241 gtctgctatg tccgtacaca tctgttccac aagcatttcc tgcttttcag agaggtgggt
     3301 acattcatca atcattttca tgtagtttga gcctgtcagc ctgtcgattt cctctatcat
     3361 cacgattgac ggcatgacct gtctttccag ccattgcaga gtgcgttcca tattgacagg
     3421 tacggggttc atttcaaaag gtaaactgtc atgggaaatg aatatctccc acagcggata
     3481 gtcgggaaat tgcacaaaat cagtaataag gtaaaacacc agcccatgag ggttatatgt
     3541 caataacaat tcctcaactg cctgtactgc ttttgtacca cgcagacgaa tttcaaaacg
     3601 attgataata tccgtatgtt tctttttggt tgcctgttcc ttttgttttt catacaggca
     3661 gaagtatttt gtgcttgcct ttgaaccgat ataaagggtg cttgctaaat tacggttttt
     3721 tccgcttagt tttccaccct gcacattttc ataattttta cagcgacaat ccgcaccgcc
     3781 atttctgtat ttttcagaca aaacgggaat atccaacaga ccacacatat cattgattgc
     3841 caagtcaaaa cgtcggataa caccgtcaca gtcaagacag cggtttaaaa atgaatacca
     3901 atctctgttc tgtgcctgca agacatattc catgttgcgt gagcctgccc cttttaattc
     3961 caagaatacg cccatgtgtt cgtcatcaga agccatgacc ttgatttctc cgtaggcgta
     4021 atgctcttta tagccataat acccatagtc ataatggata aaataatctg ctttcatgcc
     4081 aagcaccttt cggataattt ccaatgcgtc agtcgttgga taacggacag acagataatc
     4141 aacgagcaga taaaaaggct tatcgcagat ataataattc agacaacgca ggataatatt
     4201 ttgtgtttcc ttgctggcgt tggcttttcc gttctcaaag cggttgatac tatatcttga
     4261 tacatgggta agacttgcaa gttctccttg tgaaagaccg cttgcaagac gtttgttctt
     4321 catttcaagt gcaaattcta aattgttcat tgtattcctc caatcccaca atagccttga
     4381 aatacctgta aacctctatt caaaaagtgt gatatgcact atcttcttaa aacttctcat
     4441 tactactcac aactttggga ttgtatggtt tttgacttgt cttttgtgaa atagtgcccc
     4501 cctgttagat aacgggggca cagacaaggc tcgcaggctc gccagccgac aaagcatttc
     4561 ttccccttga cacaaggtgt catggcggta agcctgagag gcttccgcca ccgtgtcaga
     4621 aatactttgt cagcaagttc tgttttgcct tttctaacaa gttccatttc aaccgatatt
     4681 catatctacg gatttaatca gataggtatt tctttttgaa tgtttttata tttcattcta
     4741 ttgtgcttat tcgattacaa gaacatagta attcttatat gcttatttgt caatatcttt
     4801 ttttgccttt acaaatcata ttaagcatga tagacttata atagcgaaga tataacgaga
     4861 tatggagggt tatgcaatga aaataagcca tttaggaaat aatatacaga ccataagaaa
     4921 atttagggga atgaaacaac aggaacttgc ggacaaaatc ggtatcaata tgcagagcct
     4981 ttccaagatt gaaagagggt tgaactatcc tgcctatgaa acgctggaaa agataatgga
     5041 agtgctggac gttacgccca atgaattgtt gtcgggagaa tggaagtatg tcaatcaatc
     5101 tgaaaaggaa gtctgtcagt ttttaagagc cgaagaacgt ctaaatgcag aactgaaaca
     5161 tggacactat gataacttct ttgacagcga ggaagaatgg ctggaatatg agttagaaaa
     5221 gttacgggaa tatattacag actatatcaa cgggaaaagc attgaagcgt ccgaccttta
     5281 cccaatcaaa gaatttatcc agcatttgaa gtttcaaaaa ttattagacc gctatgatga
     5341 tttatacagt atggatatgt ttggggaaag catagaggga cataagtata agacacctta
     5401 tcaagtcgtg aaaatgataa acccgaactc aaaagaagat atggaactgt tacgagaagt
     5461 attaaggaat aacaattttg atgatgagga cgagtaactt ttttcctctt gcgagaagat
     5521 acagcaaaca aaaagcccga tcaagagtgc aaagcaccac caatggtggc aaagctcttg
     5581 acagggcttt ttcttttctg ttgtgggcaa tcaagaggaa gaaagggata aaatctgatt
     5641 tgtggtaaat caggcaaagg caacgcaggt atctcaattt gatttggtac ttgaaaagaa
     5701 cgggagtaat tggaagatta tagaataaca aatattggta cattattaca gctattttgt
     5761 aatcacgtac tctctttgat aaaaaattgg agattccttt acaaatatgc tcttacgtgc
     5821 tattatttaa gtatctattt aaaaggagtt aataaatatg cggcaaggta ttcttaaata
     5881 aactgtcaat ttgatagtgg gaacaaataa ttggatgtcc ttttttagga gggcttagtt
     5941 ttttgtaccc agtttaagaa tacctttatc atgtgattct aaagtatccg gagaatatct
     6001 gtatgctttg tatgcctatg gttatgcata aaaatcccag tgataaaagt atttatcact
     6061 gggattttta tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata
     6121 ttggagtttt agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata
     6181 acagtggagc gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata
     6241 cgcttttaga acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg
     6301 aaaatacgaa ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat
     6361 atcgttcatt atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac
     6421 aagcacaaac tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt
     6481 ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag
     6541 agaaactttc tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatatgtgtg
     6601 tgacgaactt taccgaatct gaacaatggg atacggtaat agagggaaac gatgaccttt
     6661 tagagaaata tatgtccggt aaatcattag aagcattgga actcgaacaa gaggaaagca
     6721 taagatttca gaattgttct ctgttccctc tttatcatgg aagtgcaaaa agtaatatag
     6781 ggattgataa ccttatagaa gttattacta ataaatttta ttcatcaaca catcgaggtc
     6841 cgtctgaact ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg
     6901 catatatacg cctttatagt ggagtactac atttacgaga ttcggttaga gtatcagaaa
     6961 aagaaaaaat aaaagttaca gaaatgtata cttcaataaa tggtgaatta tgtaagattg
     7021 atagagctta ttctggagaa attgttattt tgcaaaatga gtttttgaag ttaaatagtg
     7081 ttcttggaga tacaaaacta ttgccacaga gaaaaaagat tgaaaatccg caccctctac
     7141 tacaaacaac tgttgaaccg agtaaacctg aacagagaga aatgttgctt gatgcccttt
     7201 tggaaatctc agatagtgat ccgcttctac gatattacgt ggattctacg acacatgaaa
     7261 ttatactttc tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa
     7321 agtatcatgt ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa
     7381 aaaatgcaga atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg
     7441 gtttatctgt atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc
     7501 ttggatactt aaatcaatcg tttcaaaatg cagttatgga ggggatacgc tatggctgtg
     7561 aacaaggatt gtatggttgg aatgtgacgg actgtaaaat ctgttttaag tatggcttat
     7621 actatagccc tgttagtacc ccagcagatt ttcggatgct tgctcctatt gtattggaac
     7681 aagtcttaaa aaaagctgga acagaattgt tagagccata tcttagtttt aaaatttatg
     7741 cgccacagga atatctttca cgagcataca acgatgctcc taaatattgt gcgaacatcg
     7801 tagacactca attgaaaaat aatgaggtca ttcttagtgg agaaatccct gctcggtgta
     7861 ttcaagaata tcgtagtgat ttaactttct ttacaaatgg acgtagtgtt tgtttaacag
     7921 agttaaaagg gtaccatgtt actaccggtg aacctgtttg ccagccccgt cgtccaaata
     7981 gtcggataga taaagtacga tatatgttca ataaaataac ttagtgtatt ttatgttgtt
     8041 atataaatat ggtttcttgt taaataagat gaaatatttt ttaataaaga tttgaattaa
     8101 agtgtaaagg aggagatagt tattataaac tacaagtgga tattgtgtcc tgtatgtgga
     8161 aataaaacac gattaaagat aagggaagat actgaattaa aaaaattccc cctctattgt
     8221 ccgaaatgca gacaagaaaa tttaattgaa ataaagcagt tcaaagtaac tgtgattaca
     8281 gagccagacg caaagacgca gagccgataa aatgagatta atacaatctc attttatcgg
     8341 ctctttccgt tatgtatgga ttcttttaat tagtcttcga tgtttcttgc ttcgttgata
     8401 ccgctggcta aagattccat taaggatagt tctttgtctg taaagctatc catgtatttc
     8461 tctatctgta atcgtcgggt gctttttacc aagttattag caggtaagaa aaattcatca
     8521 acggaaacat gaagtaacga tacaaggtca taaagaactt gtatgctggg gtgttgccct
     8581 ttattttcaa tattagttaa gtaccgtggg tcaatttcaa tcaatgctcc cacttgttca
     8641 cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta aaccaaaggc tctaaaatca
     8701 tatttatctt cttttttacg catagtagac cacctctata cattttattg ttcctactga
     8761 attaaaaaca ggtatagaaa aacgtgttat atggtttata ggtttatatt taataaaaag
     8821 cactactaaa cgccaataaa aaaaaccgtt atatggtagt gctatttacg ctgttaaaat
     8881 attgtatatt acttccaaat ggcggtttgt tggaggtcaa cgtcgccatg aagtacatca
     8941 tatacaataa atttccttac attgggttct tgtcaaaaaa agtcgtctat ctgcaataga
     9001 taagtacgtc caccaatgtg gttttataaa tcatatagat agaataacag aagcatgtaa
     9061 acagagaaat aaatctgttt atatgctttt ttggctattc agaacttttt tacaaagttt
     9121 atttatcagt aatgcaacaa atcccccttt cacattggga ctaagagtga aaggagataa
     9181 acgagcaagg ctcacttcct ttcctagaca gaaagggggt gagattatga ggacatcttc
     9241 tttcgagcat atcgtcagat tacagttcaa ttctttaatg ctgattgtca ttaagaacaa
     9301 agtgaaaagt cgaaacagac agcttgcaag acattctaaa cgtgaagtat tgttttgtga
     9361 aatagccgaa acgaaacaag ttgaacgtgg aacgattgat acctgttttt atgattgtgt
     9421 atcatttaaa gtcttgcact ttacaattta tgtatctgat gaaacacttg gtacagtttt
     9481 acatagacta tcagaaaaac agcgtagtgc gattttatta cgttattttc aaggaatgaa
     9541 cgacagagaa atttcagaac tgtaccatgt atcacgttct gctatttgca gtagaagaac
     9601 tagaggacta aggaagttaa aaatgctttt gagcgaaagg aagtaaagat tatggctatc
     9661 cctcttttga attgatttgc aaagcgtcaa gcggagatga aatggcagtt aaagagattt
     9721 tgaagtttta tgacggttat atttctaaat tatgcttgcg tcctttttac cactctgaaa
     9781 gtggtaaaat cattatgcag gttgatgaag aactaaaagg agaaattcat acagacatga
     9841 tgaaagcaat attgaaattt gaaatcggag tgaagtaatc aactgtataa aaaaatcggg
     9901 agatacacag tttcgtgtta tctcccttag tagttttacg gtcatatcaa ggctcattca
     9961 atcgtggtaa attcgtggta agatgagtgt ttttctatac ttcaaaatgc ttgaatgtat
    10021 agtgtttatg cggataattg aaaattagat ataaaattta ataaaaacca aaaatgcacg
    10081 gtcgccatga agggaccgtg tatttttatc ttcttatttt ctcacctaat cgtatcagcc
    10141 gcatcgcttg ttttcctgca caaacaagca atcgctctac ctgttccatc gcataagaaa
    10201 gtaccatcgg ttcattgatg atatcaagta catagtcgat accaccctca cttaattgag
    10261 cttgattttt cttctccata ttatgtagtt gtgggacaac tttctgacaa tcagcaattg
    10321 tgtcaagaac ggatcaatcc tttattggag ttgctcgaaa gggtcttctc ttattacgga
    10381 caggctttgc tcactgtaca tagacaacaa atcattatcc tagtcaatcg gatttccaga
    10441 gcctcattgc tatcattgct tgataaaatc cagacaaagt ttaataaaat gtatcagtta
    10501 cagctaaact ttggaattgg gtcgctttgt tatacagagc aagagacacc gcaatctttc
    10561 cttcatgcca aacaagtttg cgaatggata gctttccatc aatctgtgaa cgagatccgc
    10621 ttttttgaag atttagacct cgggatcgtt ttaccagcaa tcccgtcaga tcaacgaaca
    10681 ttatatgtaa aacgtatttt gaaaagcctg acagaagaag aagtacattt gttcaagaag
    10741 actttggcat gcttcagtaa aaataacgga agcatcaaaa attgctcaga agaattattt
    10801 attcataaaa acaccttgca atatcgattg aacaagttcc attctctcac tggttatacc
    10861 cctagaaact atgacgatta tcatattcta aagctggctt ttttgttggt acaaacatga
    10921 caactacaaa aaaaccaggc gaacatatga ttgttcgtct ggtttttaat tattaatgac
    10981 ttagcgtttt tactagaaaa actacttccg ctttttcccg ccaaaagcat ctttaaactt
    11041 atcgaagaaa ccgtcttctt gctgttcttc aactttgatg ccgccagctt cagcgaatgc
    11101 acgtaatgct tctctttgtt gttcatttag atttttcggt gtgatcagtt ttactttgac
    11161 atgttggtcg cctgttgcac caccacgtaa cttcggagcg ccttttccac gtaaacggaa
    11221 gttcgtacct gtctgtgttc cagcagggat cttcaatttc acatctccat ggacagttgg
    11281 tacttggact tcatccccca aagctgcttg tacaaaactt aaaggcaatt catagtaaat
    11341 ttcagaacct tcacgatcaa agatatcact ttcttctaca cggaagacca catacaaatc
    11401 accaaatggt ccgccgtttt ctccagcttc tccttgacca gctaaacgca tttgttgtcc
    11461 ttcttctaca ccagctggta cattcacttt aactgaatgt gctttcttct catgtcctga
    11521 gccatgacag gttggacaag gttctttgat ttctttccct gtcccatgac aaacatcaca
    11581 agtttgacgg ctcatcactc gtccaagtgg tgtttggcgt tctacattaa tcgtacctgc
    11641 accatgacat ttatgacaag tagttggttg tgtgcctggt tttgcaccgt tcccaccaca
    11701 agtatgacag atttcttccc gattatactt gatttctttt tctacaccaa aaatcgcttc
    11761 ttcaaaagac aagtcgaccg tatattgcaa atcagcacct tgacgaggcg catttggatc
    11821 gactgaccga ccgcctcctc caaagaaaga gtcgaagata tcttcaaaac caccgaagcc
    11881 gccaccagag aagccgccga atccaccaaa tccgccacca gctccaccag caccgtaatt
    11941 tggatctgtg cctgcatgcc cgtattggtc atatgcagcg cgtttttgcg ggtcacttaa
    12001 aatctcatat gcttctgata cttctttgaa tttttcttcc gcatcaggtt ctttattgat
    12061 gtcaggatgg tattttttcg aaagcttacg ataagctttc tttatttcat cttctgatgc
    12121 tccttttgag agccctaaga cttcataata atcacgtttt gttgccatag gctttcctcc
    12181 tattggttct catcattttc acgtcgcttg ttatcatatc atattttgtt tgaaacaaaa
    12241 accgatactt caaactattt ttcaaaagaa aaagccaaag tagcaacttt ggccttctct
    12301 cgaaattcag tgaactgatt atttatcgtc gccatcgact tcttcaaaat cagcatcaac
    12361 gacatcatct gctccgcctt gagctgcttc tgggttttct tgagcttgtt gttgtgccgc
    12421 ttgttcgtat agtttaacag tcaagttttg aacgatttcg ttcaatgcat cgcgtttagt
    12481 tttcatttct tcgatgttgt ttgcttcaac agcggctttc aattcatcgc gagctgtttc
    12541 tgcttttttc acttcttctt cgtccacttt gccttctaat tctttcaatg ttttgtccac
    12601 agagaatagc aatgcatcta catcattacg taggtcaact tcctctttac gagctttatc
    12661 tgcttctgca tttgcttctg catcttttac catacgttcg atttcttcgt ctgttagacc
    12721 tgaagaagat ttgatcgtga ttttttgttc tttttgtgta cctagatctt tcgcagatac
    12781 atttacaatc ccgtttttat caatatcgaa tgttacttcg atttgtggaa taccacgagg
    12841 agctgctgga atatctgtca attggaatct tcctagagtt ttgttgtcag cagccattgg
    12901 gcgttcacct tgtaatacat ggatgtctac tgctggttga ttatctgccg cagttgagaa
    12961 tacttgtgat ttacttgttg gaatcgttgt attgcggtca atcaatttag tgaacactcc
    13021 acccattgtt tcgataccta atgacaatgg tgtaacgtca agtaaaacaa catctttcac
    13081 atcaccagtg atcacgccac cttggatagc agcacccata gcaactactt catcagggtt
    13141 aactgatttg tttggttctt ttcctgtttc tttacgtact gcttctacaa ctgcagggat
    13201 acgagtagat ccaccgacta aaataacttc gtcgatttct gattgtgata aacctgcatc
    13261 ttttagcgct tgacgtactg gtacttttgt acgttctact aagtcagctg ttaattcatc
    13321 aaattttgca cgtgtcaatg tcatttccaa atgcaatggt ccagattctc ctgctgtgat
    13381 aaatggcaag ctgatttgtg tgcttgatac gccagataaa tcttttttcg ctttttcagc
    13441 agcgtctttc aagcgttgta aagccatttt gtcttgagac aagtcaatgc tgttttcttt
    13501 tttgaattct gctaccatgt agtcgatgat tttgttatca aagtcgtccc cacctagatg
    13561 gttgtcgcca gctgttgaca atacatcaaa gacaccgtct cctagttcaa ggatagatac
    13621 gtcaaaagta ccgccaccaa ggtcgaatac taagattttt tcgtcgcgat ctgttttatc
    13681 taaaccatag gcaagagcag ctgcagttgg ttcattgacg atacgttcta cttctagacc
    13741 agcgatctta ccagcatctt ttgttgcttg acgttgtgca tcattaaagt aagctggcac
    13801 agtgataact gctttttcga ctttttcacc taaatagtct tctgcgaatc ctttgatata
    13861 ttgaaggatc attgctgaga tttcttgtgg tgtatatgat ttaccttcta catctacttt
    13921 gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc gtatttgggt ttgtcacagc
    13981 ttgacgtttt gcaacttccc ctacttggat ttctccattt ttgaatgata caacagaagg
    14041 tgttgtacgg tttccttctg ggttagcgat gatttttgct tcgtttcctt ccaatactgc
    14101 tactgcagag ttagtcgtac ctaagtcaat accgataatt ttactcataa ttaacgtctc
    14161 cttctttaat tgaaagtgta agttttatat ccgcacaatt attgtgcaac aatgaccatg
    14221 gttggtctta acacgcgatc atgtaatttg tagccttctt gtagtacttc tacaatcgta
    14281 tcggctggtg tatcttcagt cgctggaaca gtttgtactg cttgatgaag attaggatca
    14341 aaggcttctc ctttagcagg gattttttca attccttctt cttccagtgc gttacgcaaa
    14401 ctttccaaga ccatttccac gccttttttc aaacctgccc cttgatcatc tgacacttct
    14461 gtggctaatg cacgttccag gttgtcaatt gatggaagta attttttggc taaatcttgt
    14521 gaacggtatt tttgtaattg ctcacgctca ttttttcctc gatttgccat atttgcgatt
    14581 tcagcacgag cccgcaagta tttatcttcc atttcttcta actcagcttt taatttcgct
    14641 gtttcgaatt cttccgcttc agcttctgaa atacccgaat cttctgcagc ttcgacatcg
    14701 atttccggtt ctggctgagc atctgtcatt tctttatcta gttcttcttg attttctttc
    14761 acgatacagt cttcctttca ttattcagtc agctgtttga ttgctcttac taagaaagag
    14821 agaacgatca gctgcaaata gcttcgttat tcacattacg agtttgacaa gtcaagcgaa
    14881 cggtagtaat cagcaagttt agctgctagt tctcttcgat aaacatcgac tagaccgaac
    14941 atccgagaat aaggcatact agtaggccct aacaaggcta ttgtacctct tccatgacca
    15001 tctatctcgt aacttgcttg aatcatactc atattctgca ataaatcatt gcctaattca
    15061 gcaccaatct tgatatgaat attgctatcc atcggagtga tcatttgtgt aagtccgtct
    15121 gagtctttca taaaagaaaa catcgatttg aactgatcaa gatcttgacg gggctcataa
    15181 tccaagagat tcatttgtcc actgacataa accttatctt caaaaacatg accaagaacg
    15241 gtgtcaaata actgagacat cccttctgtc gtttggaaat atttatgaag aatcatcgga
    15301 atctccgtac gaagtttatg gtacaccgtc atcagaggat ctccgactag acggtcgttt
    15361 acaatctgta ccattttttc tagttcctga cttcccatat attcgggaag agtgaacact
    15421 tggctttcta cattccccat atctgttacg acgatagcaa taatctgtcg attattcaag
    15481 ggtacgatac gaaagccagt cagtcggcgg tcttttattt caggtcccaa tgaaaaagtc
    15541 gtatagcttg tcagctcaga taatatctca gcagattgct taataatctc attgatttca
    15601 tgaaattctc tgcctagaga ctgacgaatc agcagcaaat catcttctgc tacgcgagca
    15661 ggttttaaca aatgatccac gtaataacga tagcctaaag cagatggaat ccgcccagaa
    15721 gaagagtgag tcttcaacag caatccttca tcttctaatg ctttcatatc attgcgaatc
    15781 gttgctgagc tggccttgac tccctcagac atcaatgttt tggaaccaac gggcacgcca
    15841 ctactcgtat agtgttggat gatcagacgt aaaatgtcgt tttgtcgctt tgttaacatc
    15901 ttcctcacct ctcattagca ctcatatatt ctaagtgcta atccacttat aaatatagca
    15961 agaagccctt ctcctgtcaa gagaaagact ccatttttta gcactctaaa cacaagagtg
    16021 ctaattctaa ataattgatg taaatccagt tatatcaacg tttcaatagt cctttattgt
    16081 atttttgaac tttttttatt taaattcaga taaaaaacaa atattcttta aactattttt
    16141 caaacaaaaa gcgctcaaac acattgttcc ccaaaaaggt ccctcttggt gtcaaataaa
    16201 cacggtcttc ttcattaaca agtaaccctt cttgctctaa ttcccttagc acatcaccgt
    16261 acaccgattc aattgttttt tgatagcgat catagaaatg ttttttagta atccctgttt
    16321 ttttcttag
//
LOCUS       DAYYBH010000241        13213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.guided.071, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000241 DAYYBH010000000
VERSION     DAYYBH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.guided.071"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHAUG_003079"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHAUG_003079"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8482056.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACHAUG_003080"
     CDS             complement(2159..3031)
                     /locus_tag="ACHAUG_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8482057.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACHAUG_003081"
     CDS             complement(3373..4245)
                     /locus_tag="ACHAUG_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8482058.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACHAUG_003082"
     CDS             4373..4942
                     /locus_tag="ACHAUG_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8482059.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACHAUG_003083"
     CDS             5009..5746
                     /locus_tag="ACHAUG_003083"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8482060.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACHAUG_003084"
     CDS             5977..7245
                     /locus_tag="ACHAUG_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8482061.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACHAUG_003085"
     CDS             7732..8196
                     /locus_tag="ACHAUG_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8482062.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACHAUG_003086"
     CDS             8180..8809
                     /locus_tag="ACHAUG_003086"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8482063.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHAUG_003087"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHAUG_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8482064.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACHAUG_003088"
     CDS             10688..11134
                     /locus_tag="ACHAUG_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8482065.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACHAUG_003089"
     CDS             complement(11333..11848)
                     /locus_tag="ACHAUG_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8482066.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACHAUG_003090"
     CDS             11965..13167
                     /locus_tag="ACHAUG_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8482067.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAYYBH010000242         8062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.guided.108, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000242 DAYYBH010000000
VERSION     DAYYBH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.guided.108"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACHAUG_003091"
     CDS             128..1564
                     /locus_tag="ACHAUG_003091"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8482068.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACHAUG_003092"
     CDS             1753..4200
                     /locus_tag="ACHAUG_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8482069.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHAUG_003093"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHAUG_003093"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8482070.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACHAUG_003094"
     CDS             5487..5693
                     /locus_tag="ACHAUG_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8482071.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACHAUG_003095"
     CDS             complement(5690..6793)
                     /locus_tag="ACHAUG_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8482072.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACHAUG_003096"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACHAUG_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8482073.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DAYYBH010000243         7044 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.guided.113, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000243 DAYYBH010000000
VERSION     DAYYBH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.guided.113"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..1170
                     /locus_tag="ACHAUG_003097"
     CDS             259..1170
                     /locus_tag="ACHAUG_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFM8482074.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1323..1808
                     /gene="vanZ-A"
                     /locus_tag="ACHAUG_003098"
     CDS             1323..1808
                     /gene="vanZ-A"
                     /locus_tag="ACHAUG_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFM8482075.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2327..2611
                     /locus_tag="ACHAUG_003099"
     CDS             2327..2611
                     /locus_tag="ACHAUG_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8482076.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2977..3276
                     /locus_tag="ACHAUG_003100"
     CDS             2977..3276
                     /locus_tag="ACHAUG_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8482077.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            3319..4809
                     /locus_tag="ACHAUG_003101"
     CDS             3319..4809
                     /locus_tag="ACHAUG_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8482078.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            5157..5327
                     /locus_tag="ACHAUG_003102"
     CDS             5157..5327
                     /locus_tag="ACHAUG_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8482079.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            5341..5958
                     /locus_tag="ACHAUG_003103"
     CDS             5341..5958
                     /locus_tag="ACHAUG_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8482080.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            5958..6896
                     /locus_tag="ACHAUG_003104"
                     /pseudo
     CDS             5958..6896
                     /locus_tag="ACHAUG_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         2589 a         1042 c         1334 g         2079 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattc aaggaggtaa ggatggcgga atgaaaccaa cgaaattaat
     1201 gaacagcatt attgtactag cacttttggg gtaacgttag ctttttaatt taaaacccac
     1261 gttaactagg acattgctat actaatgata caacttaaac aaaagaatta gaggaaatta
     1321 tattgggaaa aatattatct agaggattgc tagctttata tttagtgaca ctaatctggt
     1381 tagtgttatt caaattacaa tacaatattt tatcagtatt taattatcat caaagaagtc
     1441 ttaacttgac tccatttact gctactggga atttcagaga gatgatagat aatgttataa
     1501 tctttattcc atttggcttg cttttgaatg tcaattttaa agaaatcgga tttttaccta
     1561 agtttgcttt tgtactggtt ttaagtctta cttttgaaat aattcaattt atcttcgcta
     1621 ttggagcgac agacataaca gatgtaatta caaatactgt tggaggcttt cttggactga
     1681 aattatatgg tttaagcaat aagcatatga atcaaaaaaa attagacaga gttattattt
     1741 ttgtaggtat acttttgctc gtattattgc tcgtttaccg tacccattta agaataaatt
     1801 acgtgtaaga tgtctaaatc aagcaatctg atctttcata cacataaaga tattgaatga
     1861 attggattag atggaaaacg ggatgtgggg aaactcgccc gtaggtgtga agtgagggga
     1921 aaaccggtga taaagtaaaa agcttaccta acactatagt aacaaagaaa gcccaattat
     1981 caattttagt gctgaggaat tggtctcttt aataaatttc cttaacgttg taaatccgca
     2041 ttttcctgac ggtaccccca ctagcagcca tgcttttttt gaatctttgt aggacgtttt
     2101 aaagagaatt acccacttta aaattaaaca tgcgcttaga atcgctttag gaaacacgat
     2161 ccagtccaat aatcgtcgat aaaaactttt gaaaaaggtt ggtgaaatta cctacttttg
     2221 gaataatcac aaatcacaag tgattaatca cttgtttatt aagatattaa aagctataat
     2281 ttaaataaag cgtgaatttt attacacaaa aagagggggg agaaacttgg aactagcatt
     2341 tagagaaagc ttaaaaaaga tgagaggtac caaatcaaaa gaaaaattct cccaagaatt
     2401 agaaatgagt agatcaaatt attcacgaat agaatcagga aaatcagatc caaccataaa
     2461 aacactagaa caaattgcaa agttaactaa ctcaacgcta gtagtggatt taatcccaaa
     2521 tgagccaaca gaaccagaac cagaaacaga atcagaacaa gtaacattgg atttagaaat
     2581 ggaagaagaa aaaagcaatg acttcgtgtg aataatgcac gaaatcgttg cttatttttt
     2641 ttaaaagcgg tatactagat ataacgaaac aacgaactga atagaaacga aaaaagagcc
     2701 atgacacatt tataaaatgt ttgacgacat tttataaatg catagcccga taagattgcc
     2761 aaaccaacgc ttatcagtta gtcagatgaa ctcttccctc gtaagaagtt atttaattaa
     2821 ctttgtttga agacggtata taaccgtact atcattatat agggaaatca gagagttttc
     2881 aagtatctaa gctactgaat ttaagaattg ttaagcaatc aatcggaaat cgtttgattg
     2941 ctttttttgt attcatttat agaaggtgga gtttgtatga atcatgatga atgtaaaact
     3001 tatataaaaa atagtttatt ggagataaga aaattagcaa atatctatac actagaaacg
     3061 tttaagaaag agttagaaaa gagaaatatc tacttagaaa caaaatcaga taagtatttt
     3121 tcttcggagg gggaagatta tatatataag ttaatagaaa ataacaaaat aatttattcg
     3181 attagtggaa aaaaattgac ttataaagga aaaaaatctt tttcaaaaca tgcaatattg
     3241 aaacagttga atgaaaaagc aaaccaagtt aattaaacaa cctattttat aggatttata
     3301 ggaaaggaga acagctgaat gaatatccct tttgttgtag aaactgtgct tcatgacggc
     3361 ttgttaaagt acaaatttaa aaatagtaaa attcgctcaa tcactaccaa gccaggtaaa
     3421 agcaaagggg ctatttttgc gtatcgctca aaatcaagca tgattggcgg tcgtggtgtt
     3481 gttctgactt ccgaggaagc gattcaagaa aatcaagata catttacaca ttggacaccc
     3541 aacgtttatc gttatggaac gtatgcagac gaaaaccgtt catacacgaa aggacattct
     3601 gaaaacaatt taagacaaat caataccttc tttattgatt ttgatattca cacggcaaaa
     3661 gaaactattt cagcaagcga tattttaaca accgctattg atttaggttt tatgcctact
     3721 atgattatca aatctgataa aggttatcaa gcatattttg ttttagaaac gccagtctat
     3781 gtgacttcaa aatcagaatt taaatctgtc aaagcagcca aaataatttc gcaaaatatc
     3841 cgagaatatt ttggaaagtc tttgccagtt gatctaacgt gtaatcattt tggtattgct
     3901 cgcataccaa gaacggacaa tgtagaattt tttgatccta attaccgtta ttctttcaaa
     3961 gaatggcaag attggtcttt caaacaaaca gataataagg gctttactcg ttcaagtcta
     4021 acggttttaa gcggtacaga aggcaaaaaa caagtagatg aaccctggtt taatctctta
     4081 ttgcacgaaa cgaaattttc aggagaaaag ggtttaatag ggcgtaataa cgtcatgttt
     4141 accctctctt tagcctactt tagttcaggc tattcaatcg aaacgtgcga atataatatg
     4201 tttgagttta ataatcgatt agatcaaccc ttagaagaaa aagaagtaat caaaattgtt
     4261 agaagtgcct attcagaaaa ctatcaaggg gctaataggg aatacattac cattctttgc
     4321 aaagcttggg tatcaagtga tttaaccagt aaagatttat ttgtccgtca agggtggttt
     4381 aaattcaaga aaaaaagaag cgaacgtcaa cgtgttcatt tgtcagaatg gaaagaagat
     4441 ttaatggctt atattagcga aaaaagcgat gtatacaagc cttatttagt gacgaccaaa
     4501 aaagagatta gagaagtgct aggcattcct gaacggacat tagataaatt gctgaaggta
     4561 ctgaaggcga atcaggaaat tttctttaag attaaaccag gaagaaatgg tggcattcaa
     4621 cttgctagtg ttaaatcatt gttgctatcg atcattaaag taaaaaaaga agaaaaagaa
     4681 agctatataa aggcgctgac aaattctttt gacttagagc atacattcat tcaagagact
     4741 ttaaacaagc tagcagaacg ccctaaaacg gacacacaac tcgatttgtt tagctatgat
     4801 acaggctgaa aataaaaccc gcactatgcc attacattta tatctatgat acgtgtttgt
     4861 tttttctttg ctgtttagcg aatgattagc agaaatatac agagtaagat tttaattaat
     4921 tattaggggg agaaggagag agtagcccga aaacttttag ttggcttgga ctgaacgaag
     4981 tgagggaaag gctactaaaa cgtcgagggg cagtgagagc gaagcgaaca cttgattttt
     5041 taattttcta tcttttatag gtcattagag tatacctatt tgtcctaata tgattttagc
     5101 agtataattg acttggtgaa taggtcattt aagttgggca taataggagg agtaaaatga
     5161 aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt ggctattttt aatgagcaac
     5221 aatatgcgca agattttatc gcttacgaaa agacaatttc tgataagcaa tttgaaattg
     5281 aaaaagtaga tattgctgat tggttattgc aaccgagaga attttagagg ttggttgaaa
     5341 atggctaaaa ttggttatgc acgtgtcagt agcaaagaac agaacttaga tcggcaatta
     5401 caagcgttac agggcgtttc taaggtcttt tcagacaaat taagcggtca atcggtcgaa
     5461 cgcccacaat tacaagctat gcttaactat attcgtgaag gggatattgt tattgttact
     5521 gaattagatc gattaggacg aaataataaa gaattaacag aattgatgaa tcaaattcaa
     5581 attaaggggg caaccctgga agtcttaaat ttaccctcaa tgaatggtat tgaagatgaa
     5641 aatttaaggc gtttgattaa tagccttgtc attgaattgt acaagtatca agcagaatca
     5701 gaacgaaaaa aaattaagga acgtcaggca caaggaatcg aaattgctaa gaaaaaaggc
     5761 aaattcaaag gtcgtcagca taaatttaaa gaaaatgatc cacgtttaaa gcatgctttc
     5821 gatttgtttt tgaatggttt atccgataaa gaagttgaag aacaaactgg aatcaatcgc
     5881 cgaacgttta gaaggtatcg agcaagatat aacgtgacag tcgatcaaag aaaaaacaat
     5941 gaaaagaggg atagttaatg agtacggtta ttttagctga aaaaccaagc caggcattag
     6001 cctacgcaag tgctttaaaa caaagcacca aaaaagacgg ttattttgag atcaaagacc
     6061 cactatttac agatgaaacg tttatcacct ttggttttgg gcatttagtg gaattagcag
     6121 aaccaggtca ttatgacgaa aagtggcaaa attggaaact tgaatctttg ccgatttttc
     6181 ctgatcgata cgattttgaa gttgcaaaag ataagggaaa gcagtttaaa attgttgcag
     6241 aacttctcaa aaaggcaaat acaattattg ttgcaacaga tagcgacaga gaaggtgaaa
     6301 atatcgcctg gtcgattatc cataaagcaa atgccttttc aaaagataaa acatttaaaa
     6361 gactatggat caatagctta gaaaaagatg taatccgaag cggttttcaa aatttgcaac
     6421 ctggaatgaa ttactatccc ttttatcaag aagcgcaaac acgccaaatt gccgattggt
     6481 tgatcggcat gaacgcaagc cctttgtata cgttaaattt acaacagaag ggcgtacaag
     6541 gtacattttc actaggacgt gttcaaacgc ccaccttata ccttattttt cagcgccagg
     6601 aagccataga gaattttaaa aaagaacctt ttttcgaggt ggaagctagt ataaaagtaa
     6661 accaagggtc gtttaagggc gttctaagcc ccacacagcg ttttaaaacc caagaggagc
     6721 ttttagcttt tgtttcttct aaacaagcta aaataggcaa tcaagagggg ataattgctg
     6781 atgttcaaac caaagagaag aaaacgaata gtccgagttt gttttcttta agtagtttgc
     6841 aatcaaaagt caatcagctt tataaagcga cagcgagcca aactttaaaa gctatttctt
     6901 ttttaataac ttaaaaataa acttaatgta acagcaagca cagtcaaggt atacaccttt
     6961 gacaaaaaat agcacattct ctatcgaaaa tttttgctta ttttttaaat tattttggga
     7021 aattttccca atcccttttt ctaa
//
LOCUS       DAYYBH010000244         4802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.guided.133, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000244 DAYYBH010000000
VERSION     DAYYBH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.guided.133"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACHAUG_003105"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACHAUG_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM8482081.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACHAUG_003106"
     CDS             763..1917
                     /locus_tag="ACHAUG_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM8482082.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="ACHAUG_003107"
     CDS             2132..3100
                     /locus_tag="ACHAUG_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM8482083.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="ACHAUG_003108"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="ACHAUG_003108"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM8482084.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="ACHAUG_003109"
     CDS             4130..4738
                     /locus_tag="ACHAUG_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFM8482085.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DAYYBH010000245         4620 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.guided.135, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000245 DAYYBH010000000
VERSION     DAYYBH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.guided.135"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..607
                     /gene="dfrG"
                     /locus_tag="ACHAUG_003110"
     CDS             110..607
                     /gene="dfrG"
                     /locus_tag="ACHAUG_003110"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8482086.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(679..2631)
                     /locus_tag="ACHAUG_003111"
     CDS             complement(679..2631)
                     /locus_tag="ACHAUG_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8482087.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2638..2874)
                     /locus_tag="ACHAUG_003112"
     CDS             complement(2638..2874)
                     /locus_tag="ACHAUG_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8482088.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3276..3566
                     /locus_tag="ACHAUG_003113"
     CDS             3276..3566
                     /locus_tag="ACHAUG_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8482089.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(3838..4187)
                     /locus_tag="ACHAUG_003114"
                     /pseudo
     CDS             complement(3838..4187)
                     /locus_tag="ACHAUG_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4280..4582
                     /locus_tag="ACHAUG_003115"
                     /pseudo
     CDS             4280..4582
                     /locus_tag="ACHAUG_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1466 a          759 c          808 g         1587 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcat aatgagaaaa ggttttttga
       61 tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa tgaaagtttc
      121 tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca ttccttggag
      181 gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga taatattagg
      241 taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata ttattctgac
      301 gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag aagatgtttt
      361 tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga tttataattt
      421 gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat tcgaaggaga
      481 tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc aaaaagggat
      541 aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa aaaacttatt
      601 gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag agcttgggat
      661 tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa attgtttatc
      721 tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt taaaagggcg
      781 acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact tcggattgaa
      841 gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc tttcaagaga
      901 tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat taacatttaa
      961 tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac aatcaactga
     1021 ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg catatttgcg
     1081 atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg ttagtaattc
     1141 agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc tgatatctcc
     1201 ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt ttctgctttt
     1261 cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt ctttcgtgaa
     1321 tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc gccattcgtc
     1381 ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag gagcatcaac
     1441 atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt gaagataagc
     1501 attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc aatctaacct
     1561 atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg tatcttcatt
     1621 taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca catatcttga
     1681 taagacctca gcagatacaa ctatatacgt ttgcatatta aggtcttcaa accccgtata
     1741 tttctttgaa ccttgtcctt tcttacggac gttatttagg tagtagtgca tcttgtccgt
     1801 attaagccac atctcgttaa atttctttgt ttgaaggggt tgtgtctcat atcgttctaa
     1861 aaactccaaa caacgcctat acagccactc cagcttatga tagtaggttc caactccaat
     1921 tcctaaaata tcacaagtac gactgactga aacccttcca acaaccatct tagcgaacat
     1981 tggaagaatg gtgttctttt gttggtgata agtagtggtt tcttctcttt ttggtagaac
     2041 attagtgaac ttcttacacg ccttacattg atagcgttga gatttgcccc tacttttccc
     2101 ccttttgtaa aattgcttcg gctcattgaa tggggttgat tcttcttccg agcagccttc
     2161 tttatgaaat tgataatctg gctcaacatc ttggatgcta ttgatttcta taaggcgttt
     2221 aatttcttca acaacagacc aattagataa aggggttact gtacagtttt gcgatacacc
     2281 tctgccaata ggattgtcat tgcagtacat acctttatga cctttatctt tggaactacc
     2341 agtcatttta tatcgacttg gtttcccttt tacatctttg aatttgtgct gttctttccc
     2401 aaaattacaa caaaacggat tgatgcagta attatattga atgggatatt cagaaccgtt
     2461 ccatttaaaa gacgttggca agaacaacat tttgttatac cttgttgcaa atcgttttgg
     2521 agttaataaa tcatattgtt tagccctatc ttttaattct tcgtttgaaa tcggaatttt
     2581 aacatctaca atttcatctt ccttcgtggc taatctttta agcttggtca ttgtctatca
     2641 acccactcta tctttgatac ccattttttc aacaatccgc ttattacttc gcttgttggc
     2701 aatccattca ataaacttct tatcgtctaa aatgttcaat aagaattcat ccactgcttc
     2761 gctttcagta cgctctgcat attcagcgta ttgcttaact atcaccctta cttgctctga
     2821 tatttcccaa tccacagact tagcgttctt attttttggc tcaagaaatt gcataggaaa
     2881 atgcacccct ttttgcttag taaattgtaa ccattttaac acaccaattt gcattcctac
     2941 aataattatt tcacgttcgc atttttatat acattttaac ttacattttg tggtgtaaat
     3001 taacaacaaa cgttgattta acaataaaaa acgagtatca aattcagttg caatttgata
     3061 ctcatttagg tttacatttt cactttttgt actccaaaac tgaactactt aaatctatac
     3121 tgctgcctct tttttttagt acatgtcctt catcttcgca ggaagtgcca atatgatttt
     3181 aagaaaggta atagacaaaa agtcaaaaag ataaaacaga gtttcttctt tacaaaggga
     3241 atgtatgttc gtataatggt attgaggtga aggttatgga gaaaaaatat ggaacagtag
     3301 ataagatccg tactttgcaa gttagtgcca gaccgttgat ccgtttcagt ttaaatgggg
     3361 tcaattgttt gattgcttcc catagtttga acttcctagc tgaagtagac gaagggatga
     3421 agttagtcgt tacaggttat tataataata gaaagcaatt tgttgtgaga tcctatcatg
     3481 tgttagggaa acccaaaatt gtggtagaat atgagaaaag tctataccca agaaaaaagg
     3541 tccgacaaga tgaaagaata aactagaaaa caagcggctg agacaaaaat gtcctagccg
     3601 cttgttttgg aaagatggat gcagacgatg ggatttgaac ccacacgagc atacgctcac
     3661 aagaacctca atctatatat aactacatgt atttaagtat ttgtgatgaa tcactaaact
     3721 ttccaaactc atttgaggga tattgcttaa agattgaaca gatcaattcg aagccctatt
     3781 agtattcgct caatgggggg atcagaggcc ataaaaagag actagtcatt actagtctca
     3841 ggtagtggaa ttgtaggatt ttaaacgttc ctggagagta ccggtatgta actcgaagag
     3901 gtgattatca tagtcgtaaa agtaaattga agaagcctcg cctttcacac gagaacgtcc
     3961 tggttcgaca gttaaaccta acatttggat ttttgagaca aacgaatcta tatcttgttc
     4021 gtcaatttta aaagcaatat gattgtatgt ctttggcagt ttattcgacg aattctgcat
     4081 tactgcaatc cataggtcct taactaagaa aaatttttct ggatataatg aaaatttttt
     4141 tgttgactat cgtagatttc ttttgcatca aataaggtat ttaacaatta agtatttaaa
     4201 agataacttt taagttatct ttaagtaatg cttaaagcta tgttatactc tattaggaaa
     4261 tataagaagg agtataaaaa tgaacaaaca taatagaaaa ataacgaata acaaagaact
     4321 gatcttgtta ctaataggga tttggggtat ctttttactc tttaatatgt atatgccgac
     4381 tatgagagcg gatgacgtag tttatgcaag tagattagat gaacttggct atttgggtgc
     4441 ttcgatagaa cattataaga catggagttc aagaattatt atagaactat tcttaatgtt
     4501 cttttctaaa cactttatgc tatggaaatt attaaattct acgattatgt taggaattgt
     4561 agtattactt tgcaagtatg ttgagagtat aaaatatttt gtgtaaatga aaaaatccat
//
LOCUS       DAYYBH010000246         1470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.guided.183, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000246 DAYYBH010000000
VERSION     DAYYBH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.guided.183"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..920
                     /locus_tag="ACHAUG_003116"
     CDS             12..920
                     /locus_tag="ACHAUG_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8482090.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            917..1397
                     /locus_tag="ACHAUG_003117"
                     /pseudo
     CDS             917..1397
                     /locus_tag="ACHAUG_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          511 a          209 c          326 g          424 t
ORIGIN      
        1 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
       61 aggatgaacg tattcgaatt gtgacccttg aggggtcacg cgcaaatatt aatataccta
      121 aagatgaatt tcaggattat gatattacat attttgtaag tgatatagaa ccgtttatat
      181 ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag ccggaggata
      241 tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt gatgattaca
      301 ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta aagggcgata
      361 aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata gttccgactg
      421 atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc tgcaatgaat
      481 tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt ttatttgcta
      541 ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca tggaaggtcg
      601 gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt gaaaggtata
      661 tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc tatgaaaaca
      721 tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt gaggtggcgg
      781 aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat accagggaca
      841 tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc gctgatatag
      901 aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag atatcgataa
      961 acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg
     1021 gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga
     1081 tgaagaggat gaggaggcag attgccttga atatattgac aatactgata agataatata
     1141 tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc ataggcagcg
     1201 cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg
     1261 aaacccagga caataacctt atagcttgta aattctatca taattgtggt ttcaaaatcg
     1321 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
     1381 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg tggttctgtt
     1441 gcaaagtttt aaataaagaa taaaatccct
//
LOCUS       DAYYBH010000247         1374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.guided.187, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000247 DAYYBH010000000
VERSION     DAYYBH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.guided.187"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            184..831
                     /locus_tag="ACHAUG_003118"
     CDS             184..831
                     /locus_tag="ACHAUG_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFM8482091.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT          501 a          152 c          218 g          503 t
ORIGIN      
        1 caattgcaaa agcagattga tttttggcaa cgtgaattta ggttttggaa gtaaaataag
       61 ttttatttga taaaaattgc taattcagta taattaatat ttacgaggtg acataacgta
      121 tgaaaaaatc agaggattat tcctcctaaa tataaaaatt taaaatttag gaggaagtta
      181 tatatgactt ttaatattat tgaattagaa aattgggata gaaaagaata ttttgaacac
      241 tattttaatc agcaaactac ttatagcatt actaaagaaa ttgatattac tttgtttaaa
      301 gatatgataa aaaagaaagg atatgaaatt tatccctctt taatttatgc aattatggaa
      361 gttgtaaata aaaataaagt gtttagaaca ggaattaata gtgagaataa attaggttat
      421 tgggataagt taaatccttt gtatacagtt tttaataagc aaactgaaaa atttactaac
      481 atttggactg aatctgataa aaacttcatt tctttttata ataattataa aaatgacttg
      541 cttgaatata aagataaaga agaaatgttt cctaaaaaac cgatacctga aaacaccata
      601 ccgatttcaa tgattccttg gattgatttt agttcattta atttaaatat tggtaacaat
      661 agcagctttt tattgcctat tattacgata ggtaaatttt atagtgagaa taataaaatt
      721 tatataccag ttgctctgca acttcatcat tctgtatgtg atggttacca tgcttcacta
      781 tttatgaatg aatttcaaga tataattcat agggtagatg attggattta gtttttagat
      841 tttgaaagtg aatttaattt tatacacgta agtgatcata aaatttatga acgtataaca
      901 accacatttt ttggttgctt gtggttttga ttttgaattt ggttttgaac ttatggactg
      961 atttattcag tccatttttt gtgcttgcac aaaaactagc ctcgcagagc acacgcatta
     1021 atgacttatg aaacgtagta aataagtcta gtgtgttata ctttacttgg aagatgcacc
     1081 gaataaaaaa tattgaagaa caactagcaa aagattttaa agagttattt tattttaagt
     1141 ctttataaca tgagtgaagc gaatttttaa atttcgatag aaatttttac atcaaaaagc
     1201 cccctgtcaa aattgacgaa gggggttttt tggcgcacgc ttttcgttag aaatatacaa
     1261 gattgaaaat cgtgtataag tgcgcccttt gttttgaact tagcacgtta catcaatttt
     1321 ttaaaatgat gtataagtgc gcccttttaa attttgagtg attatatttt ttga
//
LOCUS       DAYYBH010000248         1054 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3382
            SAMEA115706676-rid22593313.guided.205, whole genome shotgun
            sequence.
ACCESSION   DAYYBH010000248 DAYYBH010000000
VERSION     DAYYBH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706676
            Sequence Read Archive: ERR13196411
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289964.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:22:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,119
            CDSs (total)                      :: 3,061
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 43 of 100
            Pseudo Genes (incomplete)         :: 52 of 100
            Pseudo Genes (internal stop)      :: 23 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706676-rid22593313.guided.205"
                     /isolate="VRE3382"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..844
                     /gene="erm(B)"
                     /locus_tag="ACHAUG_003119"
     CDS             107..844
                     /gene="erm(B)"
                     /locus_tag="ACHAUG_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8482092.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          385 a          184 c          173 g          312 t
ORIGIN      
        1 taccagtgac taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt
       61 aacttctgta tttgtttata gatgtaatca cttcaggaga gattacatga acaaaaatat
      121 aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt
      181 gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac
      241 gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa
      301 cttatcgtca gaaaaattaa aactgaatac tcgtgtcact ttaattcacc aagatattct
      361 acagtttcaa ttccctaaca aacagaggta taaaattgtt gggaatattc cttaccattt
      421 aagcacacaa attattaaaa aagtggtttt tgaaagccgt gcgtctgaca tctatctgat
      481 tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt
      541 gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa
      601 accaaaagta aacagtgtct taataaaact tacccgccat accacagatg ttccagataa
      661 atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact
      721 gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac
      781 cattacttat gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa
      841 ataattctat gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag
      901 ataaattatt agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta
      961 cggggaattt gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag
     1021 gaaaatgtcc taagtgtggc aacaatattg tatt
//
